LOCUS       DAOPCD010000001        24459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_2201_length_24459_cov_3.845271, whole genome shotgun sequence.
ACCESSION   DAOPCD010000001 DAOPCD010000000
VERSION     DAOPCD010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 24459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24459
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2201_length_24459_cov_3.845271"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..366
                     /gene="secG"
                     /locus_tag="PLQ55_00005"
     CDS             139..366
                     /gene="secG"
                     /locus_tag="PLQ55_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPK28360.1"
                     /translation="MGTLDIIFIIVSILLIIISLMQGGKSEGASGAITGGGLNIFVKT
                     KERGSEKVISYITYGLAILFFLLAILVKALG"
     gene            570..1793
                     /gene="dcm"
                     /locus_tag="PLQ55_00010"
     CDS             570..1793
                     /gene="dcm"
                     /locus_tag="PLQ55_00010"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HPK28361.1"
                     /translation="MIQIDYPKSLHKLRRTLNVSQQQLANILNVSFSSVNRWENGHYE
                     PTIIAKVKLDELFDQVKIKPIAKQEKYKSIELFAGAGGLALGLEQAGFDHIGLVEFEK
                     NAVDTLKINRPEWNVLLEDIREVCERDLEQEFNIKKGELDLISGGAPCQSFSYAGNRL
                     GLEDVRGTMFYYYATFLRKLQPKLFLFENVRGLLSHDKGRTYKTILDIFEEQGYITLY
                     EVLNAWDYSVPQKRERLITIGIRKDLFEEGIFEFPKKHKYKPTIKDVKLDVNPDKEHC
                     KRYSKYKEEIFKLVPPGGYWRDIEPETAKEYMKSCWDMGGGRTGILRRLSLDEPSLTV
                     LTSPFMKQTDRCHPLEVRPFSYRENARFQTFPDDWVFSGTLSSKYKQIGNAVPVNLAK
                     EIGLELIKTLERINE"
     gene            1793..2607
                     /locus_tag="PLQ55_00015"
                     /pseudo
     CDS             1793..2607
                     /locus_tag="PLQ55_00015"
                     /inference="COORDINATES: protein motif:HMM:NF021095.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Eco47II family restriction endonuclease"
     gene            2764..3561
                     /locus_tag="PLQ55_00020"
     CDS             2764..3561
                     /locus_tag="PLQ55_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HPK28362.1"
                     /translation="MDIKTYVLKRKQNLKERILKDGGTPSLVIIQVNDDFASNKYVAG
                     KINDAREVGISAKLIKMPETTRLDELLKKIKKLNKDPHVNGIIVQLPLPKHIDENIVK
                     EAINPAKDVDGFHPLSRFKACTPLGIINYLKDEGVEIRGKNVVIIGRSNIVGKPAAML
                     FLEEDGNVTVLHSKTSREDLKNYVANADIMVVAVGRKHFINDEHTLKKSAVLVDVGIN
                     RDEEGKLTGDIKPGRDVYLQTPVPGGVGLLTRLTLLENVMEAYLHGI"
     gene            3551..4147
                     /locus_tag="PLQ55_00025"
     CDS             3551..4147
                     /locus_tag="PLQ55_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28363.1"
                     /translation="MEFKIENSVVYGLERAVKASGNPMRTEIEGGALHEKDIKRAVLL
                     GTTKGGEGHDNFLKGILVHVDITAPFYWWKEAQRYHWFDFISSQSTMHMLLKFKIDER
                     CVPETDKRVFPILEELVNDYNAISEEDKDAKVKAWRKIVATLPSGFCLAATMTTNYQQ
                     LKTMYFQRRNHKLKEWHTFCDWCLTLPHFKEFVIKEGK"
     gene            4152..5378
                     /locus_tag="PLQ55_00030"
     CDS             4152..5378
                     /locus_tag="PLQ55_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPK28364.1"
                     /translation="MKKSDLKKYARLIVRVGANVQKGQDVIITAELEQREFVLMLIEE
                     AYRAKANRVTLDWIDDDISKLHNKYRTVKSMSHFEKWELERYQYYVDKNPVRIYITSE
                     DPDGEKGINQEKRAQVMQNIYPVIKPYRDEMENKYQWTIAGAAGTKWAKKLFPDLTAK
                     QARNKLWDAILTTSRAYGDPIANWNEHNRSMSEKSRKLNELGLISLHYTSKNGTNFKV
                     GLIPNALFLAGGETTLGSNIFYNPNIPTEECFTTPKRGEAEGKVVATMPLSYQGELID
                     GFWFEFKDGKVVDFGAKQNEELLKQMLDMDEGARYLGEVALVPKESPINQLGLLFYST
                     LYDENASCHLALGLGFSNCIRDFEKYSLEELREMGINNSMIHVDFMIGVDDLRIVGTT
                     KYGQEVVIFNEGTWAI"
     gene            5378..6715
                     /locus_tag="PLQ55_00035"
     CDS             5378..6715
                     /locus_tag="PLQ55_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28365.1"
                     /translation="MAKNKRKCRPLCFILKAVFVLLGVVVLVLGGYIGYVSLSYYRIG
                     DTSLQVLNNQTAKLSVADIGTKEFKITTYNVGFGAYDRDYSFFMDTSVFKDEYIHSQG
                     NKSNTGDRARGTSKAQVLRNINGAYDTIAALGDLDFMLYQEVDTSSTKSYKVNQYEIG
                     NKKHVDYATIYGINYHSAYLMYPLHEPIGKSNSGISTYSKYHIAKAERREFEITDAFF
                     GKFFDLDRAFTISEIPIEGSVKKLYVFNVHMSAYDKGGVIRKVQLEQLKAAIRVARDY
                     DGDNNYVVVGGDFNHDLVLDNPLVNEEYKANIFNKQETEILEANWYNYFRLDSGKHGT
                     RVPNIITGLEEDYTHDFYDMALSSHGPVNIATCRDASIKFIDKNNNGIVDNAMVTIDG
                     FLVSDNIAVAEIKTIGSGPGLQKENLPESDPRHGLGFVYSDHNPVLMSFKLQA"
     gene            complement(6716..7021)
                     /locus_tag="PLQ55_00040"
     CDS             complement(6716..7021)
                     /locus_tag="PLQ55_00040"
                     /inference="COORDINATES: protein motif:HMM:NF020438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HPK28366.1"
                     /translation="MSRGMKKFLPFASLKEQALFLAEMQQQKKRVEKPTLSEDQCAEI
                     NRVLSEYTGETVLIYYYNCGFIKTHQAKIKKIDTLYKRLVFTDLTVSFTNLLHIEII"
     gene            complement(7018..8268)
                     /locus_tag="PLQ55_00045"
     CDS             complement(7018..8268)
                     /locus_tag="PLQ55_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage repair protein"
                     /protein_id="HPK28367.1"
                     /translation="MEKVYAVIDLKSFYACVECAERGLDPFTTPLVVCDERRGDGTII
                     MAVTPYLKAKGLSNVMRKFELPRGIPYIFAKPRMELYVQKSTEFNALLLNYFAKEDWY
                     PYSIDETFIHLTPYLELYKKTPEELIEFIIKEIYDHLKLYVTVGIAPNMFLAKCALDL
                     EAKKDPRYYARWTMEDVKTKLWQIKPLHKIWGIGKRYERRLHKLGLFTMGDIAKCPKA
                     KLKEEFGIMGEELWEHANGIDNTDLSEKYVPLDKSLTSGQVLFRDYEVNEAPVLIREM
                     TDELCMRMRKNNLKAKKVGLSIMTSKPESSTYSKSMSLLLPTCSNTELFRAFMEVFNK
                     FPLEGAKIRSLHLSVSGLSSDPYEQLSLIEDYVTQNTKEQRMWETLDELHARYGKDKI
                     MRANALTRASTYLRRTKQIGGHNK"
     gene            8407..8482
                     /locus_tag="PLQ55_00050"
     tRNA            8407..8482
                     /locus_tag="PLQ55_00050"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8440..8442,aa:Ala,seq:cgc)
     gene            8809..10149
                     /locus_tag="PLQ55_00055"
     CDS             8809..10149
                     /locus_tag="PLQ55_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008402275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK28368.1"
                     /translation="MYLKRKIDEYLVNWKKDNNKMPLIVKGARQVGKTESIKKFAENN
                     YENVIYINFVEEPIYKGITADGYKTDAIIKIMSRIDPLKVFTPYKTLIFFDELQDFPD
                     ITTALKFFKLDGRFDVICSGSLLGINYRIIESNSVGYKVDYEMNSLDFEEFLWAKGYS
                     DDVVRDMLKHMCELKPFNDVDMKIFNDLFLDYAILGGMPAVVRLYIENGTFEKTLELQ
                     KQLILDYKEDVRKYVVGLDQTRILNVFNHIAPQLAKDNKKFQITKVGKNARFADYRGC
                     IEWLKDSGVINVCYGLNYPELPLKGNYDLNKYKIYFFDTGLLVAQLDEEAQLDLRANK
                     NLGVYKGALYENIAAEALVKQSYELYYYKRNDATLEQDFFVRDIDSLIPLEVKANGGT
                     SKSLDTLISSDKYEDIKYGIKFAKSNIGYKDNIYTFPHFCMFLLKRYLNFKMEK"
     gene            10439..10630
                     /locus_tag="PLQ55_00060"
     CDS             10439..10630
                     /locus_tag="PLQ55_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28369.1"
                     /translation="MMKKGAIGAANVVKEKLRIENIINATLKPSADYRLDDFLIDDKA
                     LKTEGEGRNLVYVLNNRTY"
     gene            complement(10694..11737)
                     /locus_tag="PLQ55_00065"
     CDS             complement(10694..11737)
                     /locus_tag="PLQ55_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28370.1"
                     /translation="MKKTFTLMTLALGATLVLGGCTKPNTEATAVIKSQTMAFEATTA
                     LTLVGGFETVPEVKALQKSLAAEGVAEPDIPVATLDILFNNGVDFQINREDSDLAGYT
                     YVDTISFNIGTDSRIEYKLYYNLETAEEEIDDDSDDDELLNNEVASKARYGENKHNGK
                     DDDDDDTVCNGGGTCTNDGEEGDHNKEQDRNRNRNKDKHYRVTGLALVGEEEYKFMSK
                     TTSEVDADETETKLRFMLWKDDNNFISIKQEIEVEGTPGTPDYEYEEEFKYLVVENGE
                     VVKSFKLELENEDNELKLEVRIDGVKYEVEYEVIDDRTFIHVKVQGTEYTYEKVITTN
                     EETGEVTVSYVLQ"
     gene            complement(11828..12892)
                     /locus_tag="PLQ55_00070"
     CDS             complement(11828..12892)
                     /locus_tag="PLQ55_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28371.1"
                     /translation="MNDDKIKKIIQENVTKPEIKLSAAQILAMHEERKRAKEKPKRIP
                     FFKRPTFKYSLGFLSAALVIGITVYALRDTIVPPIISTSEGGETSTPSSGEVSTPSVP
                     VSEHNSDKIPHGKEGEFVFMTSSALNYAPEGSIPGGMGLIKPWYSISLSDPEKLELEA
                     ALDETLPLVSDFFSLDKGFNYQKNKGNYAGTYGTYTHEFIINEYTRIIGNVEIEEDDT
                     ETETELEGEIIIHDTHFHFEGETEIDTADNETDISLKIEYNDDSYLEIESENEADEQT
                     FKYKLYLDGDEKLNVEIKSYNTHGNGNGGRCAEVKTKYNNEDYEFYITKKSGNYVIEY
                     LEMIIIATETEDKKFIYSYE"
     gene            complement(12885..13436)
                     /locus_tag="PLQ55_00075"
     CDS             complement(12885..13436)
                     /locus_tag="PLQ55_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPK28372.1"
                     /translation="MSDPKVSRIIALLNELKRGNNDNFAEFYELTKYQIFYSLLSLTK
                     NHELAEDILAETYLKFLDNLKRIKANQNPLGFLLVTARNLAYDHFKKENRVSYIEDYL
                     LESDIGASTTDKYDDSDRLLARMQEILNETEYQVVVLYVLSELTHKEIAEYLDKPLGT
                     ITWTYSNAIKKLQKGLSDYARDE"
     gene            13601..14485
                     /locus_tag="PLQ55_00080"
     CDS             13601..14485
                     /locus_tag="PLQ55_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPK28373.1"
                     /translation="MFKSKFAIDPSLLHKDSVYTLNNGVKMPVIGFGTWQAKDGEEAY
                     ESVKAALRVGYRHIDTAEAYHNEESVGRAIRDSGIPREEIFVTTKLTNTHLTYEDAKQ
                     AIDASLKRLGLDYVDQYLIHWPNPINARTFYKKRNAEVYRAMEEAYFAGKIRSIGVIN
                     FHPHHLKALLKTIKVKPATNQIYVSPSDTQPKVVAFNEKHNILTVAYSPLGTGRLLGL
                     NELNQVGTKYNKRPAQVAIRWSLEHGYLPLPKSTNASRISENFDVFDFKLSKEDIKLI
                     NSLKGKAGKAPDPDHVIW"
     gene            complement(14492..16984)
                     /locus_tag="PLQ55_00085"
     CDS             complement(14492..16984)
                     /locus_tag="PLQ55_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013371600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPK28374.1"
                     /translation="MKKRLLSLFVISVLFSNVPSTSTKIVETNAQTGITVHYRGDYTN
                     PHIYYWNPQPAIITTPSWPGEKMIKDEGSDWYTKSWPELTAINLIFNNGGQPQTSDLS
                     RTTGEYWWVGQGTGGKWYTSDPDTITEPEYTDTGKLVIHVKSPHSVPKIKYYDSVPSG
                     ISTPNAINMTAEKEGWYRFGFNKISSIKAKFTIDGSESAEYFFTNGEWYLENGVLTDF
                     KPKVTSGKSDFREETIYFLMTTRFYDGDTSNNVHCWDDAKAGNLASDPAWRGDFKGLI
                     QKLDYIKALGFSAIWITPIVENASGYDYHGYHALDFTQVDPRLESPNATYQTLINETH
                     ARGMKIIQDVVFNHSGNFGEKNIFPLFKKDYTKADTVSNLIKTDPHNLLGSDYDALDG
                     GRQYSRRINAMKEDANDFRHIYHHEKSLGWEDYNVQTGQIAGDCVDLNTENPYVSDYL
                     INAYNRYIDMGVDAFRVDTVKHISRLTFNNVFNPAFQNRGGDDFFMFGEVATRYRQVW
                     NNGNPAISTPFYTWKESKSYPWATREEREASTYQHWQDNANPATQPSSNNHLLDGNNY
                     RQVDYSKKSYMNVIDFPMHWNFKNANDAFNVAVGNDYTYADATWNVMYVDSHDYAPDG
                     APEGQRFAEPQSVWAENLSLMFTFRGIPTIYYGTEIEFQKGKPIDVGPNAPLSETGRA
                     YFGNHIEGSLTVTDFGKYTNANGAVATTLNHPLAKHIRNLNLIRRNIPALQKGQYSTA
                     GINGGLAFKRRFTDSTTDSFALVTISSSATFNNIPNGTYVDAVTGEAKVVTNGSLSVN
                     VSGKGNLRVFVLTTSKTPAPGKIAESGLTYIK"
     gene            17135..18052
                     /locus_tag="PLQ55_00090"
     CDS             17135..18052
                     /locus_tag="PLQ55_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPK28375.1"
                     /translation="MKQRHKITILTLLALPLILGFTKWEKREKDPFITARAMPFVYPS
                     LNGEPKFSSQFHFNHINRGTVLNNYRGAGVTVAIIDSGLNTTHEDFLDGATTNISNKS
                     AYVEETGTYYSNIQIQSVEEHGRSIIEDTHPEAAGHGTNVASTVGAMVNGVGTAGISA
                     NITLLVLKTNYMFTEINAAIRYAADNGADIINMSLGAYEESFIDGHGKQQTGIEGAST
                     YFQTAINYAYNKGVTVIAAAGNEKTDHYSYPASNNNVIGVGALGRNTSTTIASYSNFG
                     LHNVDIVSPGSVYVADIGFVSAYTETQGT"
     gene            18068..20494
                     /locus_tag="PLQ55_00095"
     CDS             18068..20494
                     /locus_tag="PLQ55_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPK28376.1"
                     /translation="MASAAAIYKGKYPTATPAKIEQRLKETAYDLGAAGPDATFGYGR
                     LDLSALLVDEVPVTGVEINPTSLTLRVGDTSQVSATILPENASNKNHIFISENDAIAT
                     VNEDTGVVTAISVGTTRIGVLTDDLFEAYTDVTVVAAGTPLEITGLDIVDTTINLGVG
                     ATKTLDINLTPNNANYNDLNFVSNNESVATISSAGVVTALKSGIATITASAKVGTKSD
                     TVTINVLNITNATETYYFKDQYWGATPANWTSVKDAFGYDTNRVQVTEGASGASATSP
                     KSFTNIQGITFGVSSNASKGAGTVKVYLKETATSTTKTEVGSFTISSTGGTTRRESPT
                     IVPSINNSGFIQFEVICSTNSVYIHDIKIDYQVVVPDPVAVTGVTINPKTLSLTVGEN
                     SQATFTIAPSNATNKEVTFSSLNTSVATVTSDGLITAVSEGSTTIRVTTSDGNKTDDL
                     SLTVSPAPVYEPYLNVDFSAFKKNYIFGEALALSSIMAEHRASNGTITNLTGTDLTLV
                     SGSTKELGVVPLNFSYDGLTASVNITVTNVGSYTGAVAKDPLTIKGDSFAPAISTTTG
                     TNYSSLSASCYTFAGVGLRNLDSYIMVYTSGHFRNVSALETIKSLKLDYKTGGSSASV
                     QAFRFGTTEITSASGAAHTTLTSSAGGTSATVNAPAGTTHFRIDVSSKNLQANITITF
                     ESGSTYTFTAAEQAAAYEDYFMAVTHEECLVTDVKLTTWNHLKTEFDAMLPEAQNLIR
                     TTASVEFNARYNIIVAGYGYTNFLVSGGTGLGRDLKNFENKLPILIMGLGLPLSVCFY
                     IALKKRKKTL"
     gene            20508..21530
                     /locus_tag="PLQ55_00100"
     CDS             20508..21530
                     /locus_tag="PLQ55_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HPK28377.1"
                     /translation="MRKRNSIKLLPILASLLISGCRTELSSSEKSISEGTSISSEIVS
                     SETTSVTTSSEDSSSIASSEESSVSVSSEPGVPYNVTFVTNCETMLDTIYTDVIYDMP
                     LIFREGYTLAGWYLEATFINIVSFPYYVTSDVTLYAKWTEGETAHFTFELHPNNNGYI
                     VTSYGGNAINVVVPTTYNDKPIVGLGEKLFYQNGSIKSVVLPATLTYIGMQAFKDAVS
                     LESIVIPSNVTELATDAFAGTISLAQVTLSSKLKIIGNNAFENSAFAEVTIPSLVTEI
                     SSRAFAGNTNLLSVNLISTTPPLRFANSFEGTVSTMKYYVPSSAYNSYINSPYWQAYS
                     AQIVVK"
     gene            21565..22602
                     /locus_tag="PLQ55_00105"
     CDS             21565..22602
                     /locus_tag="PLQ55_00105"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPK28378.1"
                     /translation="MKKKAITLVVLLAMLVGCSRNESSSAPSVSSEGEVSTSEVNSET
                     TSEESSVASSEELPSSEELSSEEPSSEEPPSSSENPTNYPDTPAEIEVPQSVFEYYAS
                     VDFNKTGNNLKLELYNKIKGHVSTSYNELNATMAITDRNWDLSPDPNDSDPYMVLIYA
                     SYNDTNPKKYSTRNNDVWDKEHIWAKSHGNFDNTPPAGSDMHHLRASDKRNNGSRGNL
                     DFGKANSVKSTINDYDGKPSGKTGTQTGKSGTVYEPLPQYRGDVARAMFYMATRYHND
                     TQTQLTLVDNTTSSSTGVGKFGILSTLLEWHLQDPVDEFEFRRNGLVQGYQNNRNPYI
                     DFPSLAHDVFG"
     gene            22760..24187
                     /locus_tag="PLQ55_00110"
     CDS             22760..24187
                     /locus_tag="PLQ55_00110"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPK28379.1"
                     /translation="MATILIVGLTFFFLEQSIITDVTPGYYFDPLTLSNQMMVTGIIV
                     TLVSIFSFFVIRKIRLSVNGIILCIVLTIVFTMALVNLLIMPKEQLLSIRFLDGTYHD
                     INFVLPTRMRLTAIMTLVVNFSFIFFVVLVLPNHVSFLKLVLFFTIAIVLIAFFAIGY
                     SLATEWEKYVAIVKEGPGSTYQVPASFFNNRNPYASFLLNAQVMLFFLYYKNLDKKRR
                     FFYILLQIPLVLAINFTYSKTNLLLANILLIIAFLFHLVRLLGRKKFIRFGIEVFLAF
                     LLVIGFIVFRYSPKLETTNISLFLHRTLPDEMFERVPGSFDSRKKLWYYAVSLMVANP
                     IIFVFGHGPHLSRYFYHLRMQIEIEGVMTPGFGDYHNGLVEVMHTFGASGLIIYALVF
                     LLVTILLIRRARTNRSLTFFVLLSMGVFILRSQMESLAVLLFKSEGIMASLTFVLPAL
                     YLLRKSHECGFSKELKVKKTRPEEA"
     gene            complement(24216..>24459)
                     /locus_tag="PLQ55_00115"
     CDS             complement(24216..>24459)
                     /locus_tag="PLQ55_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPK28380.1"
                     /translation="VGTILDARKLILVASGAKKAEIVQKALEGPITAQITSTAIQLYA
                     GEATIILDADAASMLSNIEHIKHTEAMSKKYGFRAE"
BASE COUNT         7881 a         4043 c         4573 g         7962 t
ORIGIN      
        1 atttaaaagt ttcctaatct ttatttgtaa agataagtat gtcatctaac aaaaaacata
       61 aatatatgta tatttaaata atgttttctt gaattaattt ataaatagtg tatattaata
      121 aagaactgga ggaagactat gggaacatta gatattatct tcattatcgt atcaatctta
      181 cttattatta tctcgcttat gcaaggtggt aaaagtgaag gggcatctgg cgctatcact
      241 ggtggcggcc ttaatatttt cgtcaaaaca aaagaaagag gaagtgaaaa agtaatttct
      301 tatattactt acggccttgc tattttgttc tttctccttg caatccttgt taaagcttta
      361 ggttaagatt attaaaacta ttaaattaac acccggcagg gtgttttttt attatgtata
      421 atattgagaa gtttttatta aatttattgt atttattagt agttgtaata aaccacagca
      481 aaaacttatt tataaataaa taaggaaagt attaaaatta taaaagtgaa ataaagtgct
      541 atattatttt tggaagcgag ataagttata tgatacaaat tgattaccca aaaagccttc
      601 ataaattaag aagaacactt aatgtttcac aacaacaatt agcaaatatt ttaaatgttt
      661 ctttttcatc ggttaaccgg tgggaaaatg gtcattatga gccaacaatt atcgcaaaag
      721 taaaacttga tgaattattt gatcaagtta aaattaaacc aattgcaaaa caagaaaaat
      781 ataagtcaat tgaattattc gcaggagcgg gtggattagc gcttgggctt gaacaagcag
      841 gttttgatca tattggtcta gtggaatttg agaaaaatgc tgttgatact ctaaaaatta
      901 atagaccaga atggaatgtt cttcttgaag atattcgcga ggtgtgtgaa cgtgatttag
      961 agcaagaatt taatataaaa aaaggtgaac ttgatttaat ttccggaggg gcaccatgtc
     1021 aatcatttag ctacgctggt aatcgacttg ggttagaaga tgtacgcgga acgatgtttt
     1081 attattacgc gacatttctg cgaaaattac aaccaaaatt atttttattt gaaaatgtac
     1141 gtggtttatt atcccatgat aaaggaagaa catataaaac tattctcgat atttttgaag
     1201 aacaaggata tattacactt tatgaggtgt taaatgcttg ggattatagt gttccacaaa
     1261 aaagagaaag attaattacg attgggatta gaaaagattt atttgaagaa ggaattttcg
     1321 agttcccgaa aaagcataaa tataagccga ccattaaaga tgttaaactt gatgtaaatc
     1381 ctgataaaga acattgtaaa cgctattcaa aatacaagga agagatattt aaacttgttc
     1441 ctcctggtgg ctattggcga gatattgaac cagaaactgc caaagagtat atgaagtcgt
     1501 gttgggatat gggtggagga agaacaggca tattacgtag actaagttta gatgagccat
     1561 cactcactgt actcacaagt ccatttatga aacaaaccga cagatgtcat cctttagaag
     1621 ttagaccttt ttcctatcgt gaaaatgcta gatttcaaac ttttcctgac gattgggttt
     1681 tttctggcac gcttagttca aaatataaac aaattggaaa tgctgtacca gtcaacttag
     1741 cgaaagaaat aggtttagaa ttaattaaaa cgcttgaaag gataaatgaa taatgactat
     1801 cgacgaaaag gtgaatgatg actatactct aacttttatt actcaagaaa attttgaaaa
     1861 acatgtaaat gaattaattg ataaatacta tgaaatactt agtgattatg atttaaaacg
     1921 tttcaattca aaccttatag atccaataaa attatcaatt gacaagtatt tattagacag
     1981 aacttggaaa gaaattatcg acactgaaat taatagacaa cgtgataaaa caattacgaa
     2041 tgctcttggt gattttcatc aaaatatttt taaatatatt gaccgttgtg aagttcctaa
     2101 aactgggttt gatattattt acactaacga agcgggacaa aaaatatatg tagaactaaa
     2161 aataaacata atacaatgaa ttcaaaatca gcggaacaaa cttaccgaat gatgcataac
     2221 cacataaaag aaaacccaaa tgataaatgt tttttagttg aagttatagc acaacaaagt
     2281 agaaatattg tatggagtgt taaattagat aatgaaccct gctatgatga gcgtatacgt
     2341 cgagtttcaa ttgacgaatt ttacaaaatt gttacgggtg aagaaaatgc cttttataaa
     2401 ttttgtgtgc aactaccaaa aacaataaaa aaattacttg caaataggaa agataatgta
     2461 aaaggaacga acacggtgat aacagaatta caaaatagag gcggaaattt acaaaaagca
     2521 atatttttct tagcatttgc aaactattgc ggatttacaa cagttgaaga tcagattgat
     2581 gatttattaa atggtaatca tcattgaaaa atatgtaaca aacaatttaa aactaattta
     2641 ctaagcgact attaaatttt agagtcttta gaattagctt aatcgtgcaa actcatcttt
     2701 tttattgtct tagatatcta ggcttttgct ctcgtgttat aatttatttt atgaggtgca
     2761 tttatggata ttaaaactta tgttttaaaa cgtaaacaaa atttaaaaga acgaatacta
     2821 aaagatgggg gcaccccaag tttagttatc attcaagtaa atgatgattt tgcgagcaat
     2881 aaatatgttg caggtaagat taatgacgcc cgtgaagtag gcatcagcgc aaagcttatt
     2941 aaaatgcccg aaacaacaag acttgatgaa ttgcttaaga aaataaaaaa acttaataaa
     3001 gatccacatg ttaacggcat tattgtgcaa ttaccgctgc caaaacatat tgatgaaaat
     3061 atcgttaaag aagctattaa tcccgctaaa gatgtggacg gctttcatcc gctttctcgc
     3121 tttaaagcgt gtacgccact aggtatcatt aattatttaa aagatgaagg tgttgaaatc
     3181 cgcgggaaga atgtggtgat tattgggcgc agcaatattg ttggtaaacc tgctgctatg
     3241 ctctttttag aagaagacgg taatgtaaca gtattacatt caaagacaag tagagaagac
     3301 cttaaaaatt atgtcgctaa tgcggatatt atggttgtcg ccgtagggcg taaacacttt
     3361 attaatgacg aacatacact taaaaagagt gcagttcttg tagatgttgg gattaatcgt
     3421 gatgaagagg gaaaattaac tggtgacatt aaaccagggc gtgatgttta tttacaaact
     3481 ccagtgccag gtggcgttgg gttattaact agattaactt tattagaaaa tgtaatggag
     3541 gcttatttac atggaattta aaattgaaaa cagtgttgtc tacggtttag agcgcgccgt
     3601 taaagcgagc ggaaatccga tgcggaccga aattgaaggt ggcgcgctac atgaaaaaga
     3661 tatcaagcga gcagtgctac tcggaacgac taaaggtggc gaaggtcatg ataatttttt
     3721 aaaagggatc ttagtgcatg ttgatattac cgctcctttt tactggtgga aagaagcaca
     3781 acgctaccac tggtttgact ttattagtag tcaatcaaca atgcatatgc tgttaaaatt
     3841 taagattgat gaacgctgtg tccccgaaac tgataagcgt gttttcccta ttttagaaga
     3901 actagtaaat gattataacg ccataagtga agaagataaa gatgcaaaag ttaaggcgtg
     3961 gcgtaagatt gttgcgactt taccaagcgg tttttgtctt gcggcgacaa tgacgacgaa
     4021 ctatcaacaa cttaaaacga tgtacttcca acgtcgtaat cacaaactta aagagtggca
     4081 tactttttgt gattggtgtt taactttgcc acactttaaa gagtttgtaa ttaaagaagg
     4141 aaaataaatg tatgaaaaaa agcgatctta aaaaatatgc gcggcttatt gttagagtcg
     4201 gagcgaatgt acaaaaagga caagatgtta ttataactgc ggaacttgaa caacgcgaat
     4261 ttgtgttaat gcttattgaa gaggcttatc gcgctaaagc aaaccgcgtg acgcttgact
     4321 ggattgatga cgacattagt aaactccata ataaataccg cactgttaaa tcaatgtcac
     4381 attttgaaaa gtgggagtta gaacgctatc aatattatgt tgataagaat cccgttagaa
     4441 tttatattac aagcgaagac cctgatggtg aaaaagggat taatcaagaa aagcgtgcgc
     4501 aagtaatgca aaatatttac cccgttatta aaccttatcg tgatgagatg gaaaataagt
     4561 atcaatggac aattgccggt gctgctggta caaagtgggc gaagaaatta tttccagact
     4621 taaccgcaaa acaagcccgc aataaattat gggacgcaat attaacgacg agccgtgctt
     4681 atggcgaccc gattgctaat tggaatgaac ataatcgaag tatgagcgaa aagtctcgca
     4741 agcttaacga attaggactc atttcactac attacactag caaaaacggc actaatttta
     4801 aagttggcct tattcctaat gcactctttt tagcgggtgg tgaaacaact ttaggtagta
     4861 atattttcta taatcctaat attccgactg aagaatgttt tacaacacca aagcgcggag
     4921 aagctgaagg caaggtcgta gcgacgatgc cgcttagtta tcaaggtgaa ttaattgatg
     4981 gcttttggtt tgagtttaaa gacggtaaag ttgtcgactt tggcgctaaa caaaatgaag
     5041 aattattaaa acaaatgtta gatatggatg aaggcgctcg ttatcttggt gaagtagcgc
     5101 tcgtcccgaa agaatcacca attaatcaat tagggctctt attttattca acgctttatg
     5161 atgaaaatgc ttcgtgccac ttagcgctag gtcttgggtt tagtaattgt attcgtgatt
     5221 ttgaaaagta ctctcttgaa gaacttcgtg aaatgggaat taataactca atgattcatg
     5281 ttgattttat gattggcgtt gatgatttac ggattgtcgg tacaacaaaa tacggtcaag
     5341 aagtagtaat ctttaacgaa ggaacctggg caatttaatg gcaaaaaata aaagaaaatg
     5401 tagaccgtta tgctttatct taaaggcagt gtttgtgctt ttaggtgtcg tggtgcttgt
     5461 cttaggtggt tatataggtt atgttagcct ctcttactac cgcattggtg acactagtct
     5521 tcaagtatta aacaatcaaa cagcgaaatt aagtgtcgct gatattggta caaaagaatt
     5581 taagattaca acatataatg ttggttttgg ggcatatgac agagattaca gtttctttat
     5641 ggacacgagt gtttttaaag atgaatatat tcattcgcaa ggtaataaaa gtaacactgg
     5701 tgatcgtgcg cgtggtacaa gtaaagcaca agtgctaagg aatattaacg gtgcttatga
     5761 cacaattgca gctttaggtg atttagactt tatgctttat caagaagttg acacaagttc
     5821 aacaaagtct tataaagtta atcaatatga aattggtaat aaaaaacacg ttgattacgc
     5881 aacaatttac ggtattaact atcactctgc ttatttaatg tacccactgc atgaaccaat
     5941 tggtaaatca aattcaggta tttcgacata ctctaaatat catatcgcta aagctgaacg
     6001 ccgtgaattt gaaattacgg atgccttctt tggtaaattc tttgatttag atcgtgcttt
     6061 cactattagt gaaataccaa ttgaaggttc agttaaaaag ctttatgtat ttaatgtgca
     6121 tatgagcgct tatgataaag gtggtgtcat tcgtaaagta caacttgaac aacttaaagc
     6181 ggcaattaga gtagcgcgtg attatgatgg agataataac tatgttgtcg ttggtggcga
     6241 ctttaatcat gacttagtgc ttgataaccc gcttgttaac gaagaatata aagcaaatat
     6301 ctttaataaa caagaaacag aaattcttga agcgaattgg tataactatt ttcgccttga
     6361 ctcaggaaag cacggaacaa gagtcccaaa cattattacc ggtttagaag aagattatac
     6421 gcatgacttt tatgatatgg cgctatctag tcacggcccg gtaaatattg ccacatgtcg
     6481 cgacgcctca attaaattta ttgataaaaa taataacggg attgttgata atgcaatggt
     6541 cacaattgat ggtttccttg ttagtgataa tatcgcggta gctgaaatta aaacgattgg
     6601 tagtggccct ggtctccaaa aagaaaactt acccgaaagc gacccgcgtc acgggttagg
     6661 gtttgtttat agtgatcata accctgtgct tatgagtttt aaattacaag cgtaattaaa
     6721 taatttcaat atgtaaaaga ttggtaaatg aaacggttag gtcagtaaac actaagcgtt
     6781 tatataaagt atcaatcttt tttattttgg cttgatgcgt cttaataaaa ccgcagttat
     6841 aataataaat taatacggtt tcgccggtgt attcgcttaa aacacgatta atttctgcgc
     6901 attggtcttc actgagtgtg ggcttttcaa cacgcttttt ttgctgctgc atttccgcta
     6961 ggaaaagtgc ctgttctttt agacttgcaa acggtaaaaa ctttttcatc ccgcgactca
     7021 tttattgtga cctccaattt gtttagtgcg tcgtaaataa gtgctcgcac gcgtgagtgc
     7081 gttcgcgcgc attattttat ctttcccgta acgcgcgtga agttcatcaa gtgtttccca
     7141 cattctttgc tctttagtgt tttgtgttac ataatcctca atgagactaa gttgctcata
     7201 agggtcgctt gaaagtccgc tgacgctaag atgaagagag cgtattttag caccttctaa
     7261 aggaaactta ttaaatactt ccatgaaagc tctaaatagt tcagtattgg agcatgttgg
     7321 aagaagaagt gacattgatt tactgtatgt actactttct ggcttactag tcataatact
     7381 taaaccaact tttttagctt ttaaattatt tttacgcatg cgcatacata attcatcagt
     7441 catttcacga attaaaacgg gtgcttcgtt aacttcgtaa tcacggaata acacttgtcc
     7501 acttgttagt gacttatcaa gtgggacata cttttcactt aagtcggtgt tatcaatccc
     7561 gttagcgtgt tcccaaagct cttcacccat aatcccaaat tcttctttta acttcgcttt
     7621 cggacactta gcaatgtccc ccattgtaaa aagccctaat ttatggagtc ttctttcata
     7681 gcgtttacca ataccccata ttttatgcag tggtttaatt tgccacaatt tagttttaac
     7741 atcttccatt gtccatcttg cgtaatagcg cgggtctttt ttggcttcta aatcaagtgc
     7801 gcatttagct aaaaacatat ttggcgctat accgacggtg acatagagtt ttaagtgatc
     7861 atatatttct ttaataataa attcaataag ttcttctggt gttttcttat aaagttctaa
     7921 gtacggtgtt aaatgaataa aagtttcatc aatcgaataa ggataccagt cttcctttgc
     7981 aaaataatta agtaggaggg cattaaattc agttgatttt tgtacatata gttccatacg
     8041 cggtttagca aagatataag ggatacctct cggaagttca aacttccgca ttacattact
     8101 taaacctttt gcttttaagt acggggtaac agccataata atagtgccgt ctccgcgccg
     8161 ctcatcgcac accactaaag gagtggtaaa gggatcaaga ccacgctctg cacattctac
     8221 gcatgcgtag aaacttttta aatcgataac ggcatatact ttttccatta ccataattat
     8281 acaagaggac actttaattt ttagacagaa ggatgaacaa agttagtaaa agattttcac
     8341 cattaaaatt gataagcgat agcccttgca attatttact agtttgataa acttttagtt
     8401 agacttgggg ccgtagctca tctgggagag cgcatcgttc gcaacgatga ggtagcgagt
     8461 tcgatcctcg tcggctccac caaagtaaaa gtattagctt ccgctcaggg agctattttt
     8521 ttatttatta ataacaaaaa ccatttattt tgaaaaccaa aaaagaagca aaatttataa
     8581 aattgtgtca ttaattagat aagcgacgaa aaatattgat tatatgaatg tttacatgtt
     8641 tttaaatata aaactccctc atttttttgt aaattctgcc aaaaagtcgc tccttttttt
     8701 gtaaattttg gaaaaatctc gctccttttt ttgtaaaaag gcttgttata tatagattat
     8761 agtgttaata ttgaattaca aatttaatga tgatgaggga tgattatgat ttatcttaaa
     8821 cgtaaaattg atgagtattt agttaattgg aaaaaggata ataacaaaat gcctttaatt
     8881 gttaaaggtg cacgacaagt tggtaagact gagtcaatta aaaaatttgc cgaaaataat
     8941 tatgaaaatg tcatttatat taatttcgtg gaagaaccaa tatataaagg tattactgcg
     9001 gatgggtata aaacagatgc gattattaaa attatgtcac ggattgatcc gcttaaagta
     9061 tttaccccat ataaaacact aatctttttt gatgaattac aagattttcc tgatatcacc
     9121 actgcgttga agttttttaa attagatggt cgctttgatg tcatctgtag cggttcactg
     9181 cttggtatta attataggat aattgagagt aatagcgttg gctataaagt agattatgaa
     9241 atgaattcac tcgattttga agaatttttg tgggccaaag gctatagtga tgatgttgtg
     9301 cgtgacatgc taaaacatat gtgtgagttg aagccattta atgacgttga tatgaaaatc
     9361 tttaatgatt tatttttaga ttacgctatt cttgggggga tgcctgcggt tgtcagactt
     9421 tatattgaaa atggtacatt tgaaaaaaca cttgaattac aaaaacaact tattttggat
     9481 tataaagaag atgtccgcaa atatgttgtt ggtttagatc aaacacgcat tttgaatgtt
     9541 tttaatcata ttgcaccaca attagcgaaa gacaataaaa aatttcaaat tacaaaagtt
     9601 ggcaaaaatg cccgttttgc tgattaccgc ggctgtattg aatggttaaa agattcgggg
     9661 gttattaatg tatgctatgg gttaaattat ccagaacttc cgcttaaagg caattatgat
     9721 cttaataaat ataaaatata tttctttgat actggactac ttgttgcaca attagatgaa
     9781 gaagcacaac ttgacttacg cgctaataaa aatttaggtg tctataaggg tgcattatat
     9841 gaaaatatcg ccgccgaagc attagttaaa caaagttatg aattatatta ttataagcgt
     9901 aatgacgcta ctttagaaca agacttcttt gtacgcgata ttgatagttt aatcccactt
     9961 gaagttaagg ctaatggagg gacatcaaaa tcccttgata ctcttattag cagtgacaaa
    10021 tatgaagata taaaatacgg cattaaattt gctaaaagta atattggtta taaagataat
    10081 atttatacat tcccgcattt ttgtatgttt ttattaaaga gatacctcaa ttttaaaatg
    10141 gagaaatagc tattaaataa taaattctaa aaaaaacaaa agaatagcgg acaaagctcc
    10201 tatttttatc atttaacacg accacaggtc gtttcattta caaatatttg caacgagaga
    10261 aaattattcg ttgcaaacat agttgtttta gattctaatt agcctagaag gtgaagtgct
    10321 atgaaacaac aacataaata aactaaaaag ttagaattaa aaagccaata ttcagacaca
    10381 ctcttaaaaa ctattagtgc gttcgctaat tttgatggtg gcgaaattat tattggttat
    10441 gatgaaaaaa ggtgctattg gtgctgctaa tgtagtaaaa gagaaattac gaattgaaaa
    10501 tataattaat gctaccctta aaccttcagc ggattatcgt ttagatgact ttttaattga
    10561 tgataaagca ttaaaaactg aaggcgaagg ccggaaccta gtatatgttc taaataatag
    10621 aacatattaa taaattaaaa aggaccatgt tgccatggtc cgattattac tatggctcta
    10681 ttaatgactt tgcttattgg agaacgtaac taacagttac ttcaccagtt tcttcgttcg
    10741 tggtaataac tttttcgtag gtgtattcag tgccttgaac tttgacgtgg atgaatgtcc
    10801 gatcatcaat tacttcatat tcaacttcgt atttaacgcc atcgattcta acttcgagtt
    10861 ttaattcatt atcttcgttt tcaagttcaa gtttgaagct tttcacgact tcgccgtttt
    10921 ctacgactaa gtatttgaat tcttcttcgt attcataatc tggggttcct ggagtgcctt
    10981 caacttcgat ttcttgttta atgctgatga agttgttgtc atctttccag agcataaagc
    11041 gtaattttgt ttcggtttcg tcagcgtcaa cttcacttgt tgttttagac atgaatttat
    11101 attcttcttc gccaacgagc gctaagccag taacgcggta gtgtttgtct ttgttgcgat
    11161 tacgattgcg atcttgttct ttattatggt cgccttcttc gccgtcgtta gtgcatgtgc
    11221 caccgccatt gcagactgtg tcatcgtcgt cgtcatcttt gccattatgt ttgttttcgc
    11281 cgtagcgtgc tttacttgcg acttcattat ttaataattc gtcatcgtca ctatcatcat
    11341 caatttcttc ttccgctgtt tctaaattgt aatagagttt gtattcaatg cggctatctg
    11401 ttccgatgtt gaaggagatc gtatcaacat aggtataacc cgctaaatca ctatcttcac
    11461 gattaatttg gaagtcaaca ccattattga ataagatgtc aagtgttgca actgggatat
    11521 caggttcggc aacgccttcc gcggcaagtg atttttgtaa ggctttcacc tctggtacag
    11581 tttcgaatcc gcccactagt gtgagagcgg tcgtggcttc aaaggccatt gtttgacttt
    11641 taattacggc tgtggcttca gtatttggtt ttgtacagcc accaagcacg agtgttgctc
    11701 caagagcgag agtcatgagt gtaaacgttt ttttcataag tagttcctcc ttttttccgt
    11761 tcacaataaa tacgaattaa aattcttttt tattggaatt atttaaaaat tatttttatt
    11821 ttatttttta ttcataagaa taaataaatt ttttatcttc agtttctgtt gcaataataa
    11881 tcatttcaag atattcaatg acataattac cgctcttttt tgtgatgtaa aattcataat
    11941 cttcattgtt atatttcgtt tttacttctg cgcaacgacc accattacca ttaccatgcg
    12001 tgttataact tttaatttca acattaagtt tttcgtcgcc atcaagatat agtttatatt
    12061 taaatgtttg ttcatcagct tcgttttcgc tctcgatttc taaatatgaa tcatcattat
    12121 attcaatctt taaactaata tctgtttcgt tatcagcagt atcaatttct gtttcgcctt
    12181 caaaatggaa atgtgtgtcg tgaataatga tttcaccttc aagttccgtt tccgtttcgg
    12241 tatcatcttc ttcaatttca acattaccaa taatgcgtgt gtattcatta ataataaatt
    12301 catgcgtgta tgtaccatat gttcccgcat aattcccttt attcttctga taattaaaac
    12361 ctttatcaag actaaagaag tcactcacta agggtagcgt ttcatctagc gctgcttcta
    12421 gttctaattt ttcaggatca ctaagtgaga tactatacca tggtttaatt aatcccattc
    12481 caccaggaat tgagccttca ggggcgtaat taagggcaga agaagtcata aatacaaact
    12541 caccttcttt accatgtggg attttatccg aattatgttc acttactggt acactaggag
    12601 tggagacttc accactgctt ggggtcgaag tttcgccacc ttcgcttgtg ctaataattg
    12661 gggggacaat agtgtctctt aaagcataga ctgtaatccc gattacaagc gctgcactta
    12721 agaaacctag gctatattta aatgttggac gtttaaagaa aggaatgcgt tttggctttt
    12781 ctttagcgcg tttacgttct tcatgcatgg ctaaaatctg agcagcggaa agcttaatct
    12841 caggttttgt cacattttcc tgaatgattt ttttaatttt gtcgtcattc atcccgtgca
    12901 taatcactta atcctttctg taatttttta atagcgttgc tatatgtcca tgtaatggtg
    12961 cctaatggtt tatctaagta ttcggcaatt tctttgtgtg ttaattcact taaaacataa
    13021 agcacgacaa cctggtattc tgtttcgttt agaatttctt gcatcctggc gagtaagcga
    13081 tcactatcgt catacttgtc agttgtgctc gcaccaatat cgctttcaag gagataatct
    13141 tcaatataag aaacacgatt ttcttttttg aagtggtcgt aggctaagtt tctagcggtc
    13201 acaagcaaga agccaagagg attttgattg gccttaatgc gtttgagatt gtctaaaaac
    13261 tttagataag tttcggcgag aatatcttcc gcgagttcgt gatttttcgt cagcgataat
    13321 agcgaataga agatttgata ctttgtgagt tcgtaaaact ccgcgaagtt atcattattc
    13381 ccgcgtttta attcattgag taatgcaata atacgactga cttttgggtc actcatttgc
    13441 acttcctcac tattaatacg attaagttca tcattttatt ggtaatattt aaattttttt
    13501 ccacgactat tataacaaac ttaagacttt gtcttataaa atcacacatt ctctgaaaaa
    13561 agtataattt atttgttaaa atatttatag gaggggtcag atgtttaaat caaaatttgc
    13621 aattgatcct agccttttac acaaggatag cgtttataca ctaaataatg gagtgaagat
    13681 gcccgtcatc ggttttggca cctggcaagc aaaagacggc gaagaagctt atgaaagcgt
    13741 aaaagcagcg ctgcgtgttg gttatcgcca tattgacact gccgaagctt atcataacga
    13801 agaaagtgtt gggcgcgcta ttcgtgatag cggtatcccg cgcgaagaaa tatttgttac
    13861 cactaaatta acaaatactc atttaactta tgaagacgca aagcaagcga ttgatgcaag
    13921 tttaaagcgc ttagggcttg attatgttga ccaatattta attcactggc caaatccaat
    13981 taatgctcgt actttttata aaaagagaaa tgcagaagta tatagagcga tggaagaagc
    14041 ttattttgct gggaaaattc gctcaattgg tgttattaac tttcatcccc atcacttaaa
    14101 agcattactt aaaacaatta aagtaaaacc cgctacaaat caaatatatg taagtccaag
    14161 tgatacacaa cctaaagttg tcgcctttaa tgagaagcat aatattttga cagtagccta
    14221 ttctccactt ggcactggtc gactattagg gttaaatgaa cttaatcaag ttggaacaaa
    14281 atacaataag cgtcccgctc aagtagcgat cagatggagt ttagaacatg gttatttgcc
    14341 gttacctaaa tccacaaacg catcacgcat tagtgaaaac tttgatgttt ttgatttcaa
    14401 gttaagtaaa gaagatatta aattaattaa ttcattaaaa ggaaaagcgg gcaaagcccc
    14461 tgatccagat catgtaattt ggtaaatata attatttaat ataagttaag ccgctttctg
    14521 ctattttgcc tggtgcaggt gttttagaag tagttaaaac gaacactctt aaattgcctt
    14581 taccagatac attcacggat aaagaaccat tagtgacaac ttttgcttca ccagtaacag
    14641 catcaacata agtaccattt ggaatattat taaatgtagc actacttgaa attgtgacaa
    14701 gtgcgaagct gtcagtggtg ctatcagtaa atcggcgttt aaacgctaaa ccgccattaa
    14761 taccagcagt actatattgt cccttttgta aagcggggat gtttcttcta attaagttta
    14821 agttacgaat atgtttcgct aatgggtgat ttaatgttgt tgcaactgct ccatttgcgt
    14881 tcgtatactt accaaaatca gtcacagtga gtgagccttc aatatgatta ccaaaatatg
    14941 cgcgacctgt ttcactaagt ggggcatttg gtccaacatc aattggtttt cctttttgga
    15001 attcaatttc tgttccgtaa taaatcgttg ggatacctct aaaagtaaac atcagactta
    15061 aattttccgc ccatacactt tgtggttcgg caaagcgttg tccttcagga gcgccatctg
    15121 gtgcatagtc atgcgaatca acatacatta cattccatgt tgcatcagca taagtataat
    15181 cattcccgac tgcgacatta aaggcgtcat tagcgttctt aaagttccag tgcatcggga
    15241 aatcaataac attcatgtaa cttttctttg agtaatcaac ttgtcgataa ttattaccat
    15301 ctagcagatg gttgttactg ctaggttgtg ttgctggatt agcgttatct tgccagtgct
    15361 gatatgtact tgcttcccgc tcttctcgtg ttgcccacgg ataactttta ctttctttcc
    15421 aggtgtagaa cggtgtgcta attgcggggt taccattatt ccatacttgg cgataacgag
    15481 tggcgacttc cccaaacata aagaagtcat caccccctct attttgaaaa gcaggattaa
    15541 aaacattatt aaaggttaag cgtgaaatat gtttaacagt atcaactcta aaagcatcaa
    15601 ctcccatatc aatatagcga ttataagcat tgattaagta gtcactaaca taaggatttt
    15661 cagtgtttaa atcaacgcag tctccagcaa tttgtccggt ttgcacatta taatcttccc
    15721 agccaagact tttttcatgg tgataaatat ggcggaagtc attagcgtct tccttcattg
    15781 cattaatacg cctactatat tgtctaccac catcaagtgc gtcgtagtca ctaccaagta
    15841 aattatgcgg gtcagtttta attaagttac taacggtgtc agcttttgtg taatcttttt
    15901 taaatagtgg gaagatgttt ttctctccaa agttcccgct gtggttaaag acaacatctt
    15961 ggatgatttt catcccgcgt gcatgtgttt cattaattaa agtttgataa gttgcgtttg
    16021 gactttctaa gcgcggatca acttgagtaa agtcaagagc gtgataaccg tgataatcat
    16081 agcccgaagc attttcgaca attggtgtaa tccaaattgc actaaaacca agggctttaa
    16141 tgtaatcaag tttttgaatt aagcctttaa agtctccgcg ccacgctgga tcactagcta
    16201 aattccctgc tttagcatca tcccaacagt gaacattatt agatgtgtca ccatcataaa
    16261 atcttgttgt catcaagaag taaattgttt cttctctaaa atcactttta ccacttgtta
    16321 cttttggttt aaagtcagtg agtactccgt tttctaaata ccattcgcca ttagtgaaaa
    16381 agtactcagc actttcacta ccatcaattg tgaatttagc tttaatactt gaaatcttat
    16441 taaagccaaa gcggtaccat ccctcttttt cagcagtcat atttatggca tttggtgtac
    16501 ttattccgct tgggacactg tcataatact taattttagg tacactgtgt ggtgacttaa
    16561 catggattac taatttacca gtatcagtgt attctggttc ggtaatggta tcggggtcac
    16621 ttgtgtacca tttaccaccc gtgccttgcc cgacccacca gtattcgcca gtagtgcgtg
    16681 ataaatcact agtttgtggt tgcccaccat tattaaatat taagttaata gcagttaatt
    16741 cgggccacga tttagtgtac caatcacttc cttcatcttt tatcatcttt tcaccaggcc
    16801 aacttggtgt agtaataata gcgggctgcg gattccaata ataaatgtgc ggattagtgt
    16861 agtcaccgcg ataatgaaca gtaatccctg tttgtgcatt tgtttccact attttagtgg
    16921 aagtacttgg cacattacta aaaaggacac taataacaaa caaagataac aatcgttttt
    16981 tcataagtgt ttctccaacg ctaacttcat tataacaaag gatgacatta ttattttggt
    17041 aagttgaaaa taatgttttg ttacacaaaa aacttattat ttatatattt tgaatagttt
    17101 catgttatta tcttaattac tggagatttt tactatgaaa caacgtcaca aaataacaat
    17161 cctaactttg ttagccttac cacttattct tggttttact aaatgggaaa aaagagaaaa
    17221 agatccgttt attacagcac gggctatgcc ttttgtgtat ccgtctttaa atggtgaacc
    17281 aaaattttct agtcaattcc attttaatca tattaatcgc ggcactgtac ttaataacta
    17341 tcgtggtgca ggagtcacag tagcaattat tgactcagga ctaaatacaa cacatgaaga
    17401 ttttttagat ggcgctacta cgaatatttc taataaatca gcgtatgttg aagaaacagg
    17461 aacttattat tcaaatatac aaattcaatc tgtagaagag catggacgaa gtatcattga
    17521 agatactcat cccgaagctg cggggcacgg cacgaatgtc gctagtacag ttggtgcgat
    17581 ggttaatggt gttggaacag cgggtatttc agcaaatata acactcttgg tgttaaagac
    17641 aaattatatg tttactgaaa ttaatgcggc aattcgttac gctgctgata atggtgccga
    17701 tattattaat atgtcactag gcgcatatga agaatcattc attgatggcc acgggaaaca
    17761 acaaactggg atcgaaggtg cttctaccta cttccaaaca gcgattaatt atgcttacaa
    17821 taaaggtgta acagtaattg cggcagcggg aaatgaaaag actgatcatt attcttatcc
    17881 tgcgagtaat aataatgtta ttggtgttgg tgcacttggt cgtaatacat caacaaccat
    17941 cgcttcttat tcaaactttg gcttacataa tgttgatata gttagtccag gaagtgttta
    18001 cgtcgctgat attggtttcg ttagtgctta cactgaaaca caaggcacat gatttgcagc
    18061 cccaattgtg gcaagtgcag cggctattta caagggaaaa tatccgactg cgactcctgc
    18121 taaaattgaa caaagattaa aagaaacggc ctatgaccta ggtgcagcgg gaccagatgc
    18181 tacttttggt tacggtcgtc ttgatttaag tgccttactt gttgatgaag taccagttac
    18241 tggtgttgaa attaatccaa caagtttaac acttagagtt ggtgacacta gtcaggtaag
    18301 tgcaacaatt cttcctgaaa acgctagtaa taaaaaccat atctttatta gtgaaaatga
    18361 tgctattgca accgttaacg aagatactgg agtagtaacc gctattagtg taggaacaac
    18421 aagaattggt gtacttactg atgacttatt cgaagcttac actgatgtta ctgttgttgc
    18481 tgctgggaca ccgctagaaa taacaggtct agatattgtc gatacaacaa ttaatttagg
    18541 agttggtgcg actaaaacac tagatattaa tttaactcca aacaatgcaa attataatga
    18601 cttaaatttt gttagtaata atgaaagcgt tgcaactata agtagtgctg gtgttgtcac
    18661 cgcccttaaa agcggaatcg ctacgattac cgctagtgct aaagttggaa caaagagtga
    18721 tactgttact attaatgttt taaatataac caatgcgaca gaaacttatt attttaaaga
    18781 tcaatattgg ggcgctactc ccgctaattg gacaagtgtt aaagatgctt ttggttatga
    18841 tacaaatcgt gtacaagtaa ctgaaggcgc tagtggagca agcgcgacat caccaaaaag
    18901 ctttacaaat attcagggta ttacttttgg agtgtcgtct aacgctagca aaggcgcagg
    18961 tactgttaaa gtgtatttaa aagaaacagc tacttccact acaaaaacag aagtaggtag
    19021 ttttacgata agtagtactg gcggaacaac taggcgtgaa agtccaacta ttgtcccaag
    19081 tataaataat agcggtttta ttcaattcga agtgatttgt tcgacaaata gcgtgtatat
    19141 tcatgatatt aagattgatt atcaagtagt agtacctgat ccagtcgctg tcactggtgt
    19201 gacgattaat ccaaaaacat tgtcattaac tgttggcgaa aatagtcaag caacatttac
    19261 gattgcacca agtaatgcta cgaataaaga agtaacattt tcaagcttaa acactagtgt
    19321 tgctactgtt acgagtgatg gtctaataac tgctgtaagc gaagggagta cgacaattcg
    19381 cgttactact tcagatggta ataagactga tgacttaagt ttaacagttt caccagcacc
    19441 agtttatgaa ccatatttaa atgttgattt tagtgccttc aaaaagaatt atattttcgg
    19501 tgaagcttta gcgcttagtt caattatggc agaacatcgc gcaagtaatg gcacgattac
    19561 aaacttaaca ggtacagatt taacacttgt tagtggttcc actaaagaat taggtgttgt
    19621 gccacttaac tttagttatg atggccttac tgcaagtgtt aatataactg tgactaatgt
    19681 cggaagttat actggagccg ttgcaaaaga tccgctaaca attaaaggtg attcatttgc
    19741 tccagcaatt tcaacaacaa cgggcacaaa ttatagtagt ttatcagcat cctgttatac
    19801 ttttgctggt gttggattac gtaatttaga ttcgtatatt atggtctaca ctagtggtca
    19861 cttccgcaat gtttcagcac ttgaaacgat taaatcactt aaattagact ataaaactgg
    19921 tggaagtagt gcttcagtac aagcttttag atttggtaca actgaaatca caagtgcaag
    19981 tggtgcagca cacacgactt taacttcatc agctggaggc acaagcgcaa cggtaaacgc
    20041 tccagcgggt acaacacact ttagaattga tgtaagtagc aaaaacttac aagcaaatat
    20101 aacgattacc tttgaatcag gtagtacata cacatttaca gctgcagaac aagcagcggc
    20161 atacgaagat tatttcatgg ctgtaactca cgaagagtgt cttgtcactg atgtaaaact
    20221 tacaacatgg aatcatttaa aaactgaatt tgatgcgatg ctacctgaag ctcaaaattt
    20281 aattagaacg accgctagcg tggagtttaa tgctcgttat aacattattg ttgcaggtta
    20341 tgggtacact aatttcttag ttagtggtgg tacgggacta ggtcgcgatc ttaaaaattt
    20401 cgaaaacaag ctaccaattc taataatggg attaggatta ccattatcag tttgttttta
    20461 tattgcactt aaaaaaagaa aaaaaacatt ataatttaag taaggttatg agaaaaagaa
    20521 attctataaa attattacct attttagcgt cactacttat tagtggttgt cgaactgagt
    20581 taagtagtag cgaaaaaagt ataagcgaag gaacaagtat tagttctgaa attgtttcaa
    20641 gtgaaactac aagtgtgacg acttctagcg aagactcttc aagcatcgca agtagtgaag
    20701 aatcttctgt ttctgtaagc agcgaaccag gagtgcccta taatgttaca tttgttacaa
    20761 attgtgaaac aatgcttgat acaatctaca ccgatgtaat ttatgatatg cctcttattt
    20821 ttagagaagg ctacactctt gcaggatggt acttagaagc aacatttatt aatatcgtca
    20881 gttttcctta ttatgtgact agtgatgtga cattgtacgc gaaatggacc gaaggtgaaa
    20941 cagcacactt tacctttgaa ttacatccta ataataatgg ttatattgtt acatcttacg
    21001 gtggtaatgc gattaatgtt gttgtgccca caacttataa tgataaacct atcgtgggac
    21061 taggtgaaaa gcttttctat caaaatggta gtattaagtc tgtcgtttta cctgccacat
    21121 taacttatat tgggatgcaa gcttttaaag atgcagttag tttagaaagt attgttattc
    21181 caagcaatgt tactgagtta gcaacggatg cttttgccgg aacaataagc ctagcgcaag
    21241 taacactttc gagcaaatta aaaattattg ggaataacgc ttttgaaaat agcgcttttg
    21301 cagaagtgac tattcctagt ttagttactg aaattagttc acgtgctttt gctggtaaca
    21361 ctaatttact aagtgttaat ttaatatcaa ccacaccacc attacgtttc gctaattcat
    21421 ttgaaggcac tgtttcgacg atgaaatatt atgtgccttc ctcagcatat aactcatata
    21481 taaatagccc ttattggcaa gcatattccg cacaaattgt ggttaaataa atagaaaggt
    21541 ctaaaaataa ataggtaaaa aagtatgaaa aagaaagcaa ttacacttgt agtactatta
    21601 gcaatgttag tgggctgttc gcgtaatgaa tcaagtagcg caccttctgt aagcagtgaa
    21661 ggagaagtgt ccactagtga agttaatagt gaaacgacaa gtgaagaatc aagtgttgcg
    21721 agtagcgaag agctgccaag tagtgaggaa ctttcaagcg aagaaccttc gagtgaagaa
    21781 ccgcctagta gcagtgaaaa cccgactaat tatcctgata caccagcgga aattgaagtc
    21841 ccccaaagtg tgtttgaata ttacgcaagt gtagacttta ataaaacagg caacaattta
    21901 aaactagaac tatataacaa aattaaaggg catgtaagta caagttataa cgaattaaac
    21961 gcaacaatgg ctattactga tcgtaactgg gacttatcac cggaccctaa tgatagtgat
    22021 ccatatatgg tgctaattta cgctagttat aatgatacta atcctaaaaa atattctacc
    22081 cgtaataatg atgtatggga taaagaacat atttgggcga agtctcacgg taactttgat
    22141 aataccccac cagcgggaag tgacatgcat cacttacgcg ctagtgataa gcgcaataat
    22201 ggtagccgtg gtaatttaga ctttggtaag gcaaatagtg ttaaatctac gattaatgat
    22261 tatgatggta agccttctgg taaaacggga acgcaaaccg ggaaaagtgg aaccgtttat
    22321 gaaccactac cacaatatcg cggtgatgtt gcgcgtgcga tgttttatat ggcaacacgt
    22381 taccataatg atacgcaaac gcaattaaca cttgtcgata atacaacaag tagttcaact
    22441 ggagtaggta aatttggcat tttatctact cttttagaat ggcacttaca agacccagtt
    22501 gatgaatttg aatttaggag aaacggatta gtgcaaggtt atcaaaacaa ccgtaatccg
    22561 tatattgact tcccatcttt agcgcatgat gtattcggtt aataaagaaa attatagcaa
    22621 aaaaacacaa ataaaataca cacataaaat gtgtgtcatt ttaaccgcac aattatctgc
    22681 attaacattt aggaacaaat agttgtataa ttaaaaaatg agtaaagtca gggaaagagc
    22741 aaatctgttt atatactttt tggccacaat tctaattgtg ggcttaactt tcttcttttt
    22801 agaacagtca ataattacgg atgttactcc tggatattac tttgatccgc taacactttc
    22861 taatcaaatg atggtaacag gaatcattgt aacactcgtt agtatttttt cttttttcgt
    22921 tatccgtaag attcgtctta gtgtaaacgg tattatcctg tgtattgtac ttactatagt
    22981 atttactatg gcacttgtta acttgctaat aatgccaaaa gaacaattgc tttcaattcg
    23041 ctttttagat ggaacatatc acgatataaa ctttgtgtta ccaacaagga tgcgtttaac
    23101 agcgattatg acgcttgttg tgaattttag ttttattttc tttgtcgtgc ttgtgttacc
    23161 aaatcatgtg tcatttttaa aattagtgct tttctttact atagcaattg tcttaatcgc
    23221 tttttttgct attgggtaca gtcttgctac tgaatgggaa aaatatgttg caattgtaaa
    23281 agagggtcca gggagcactt accaagtgcc cgctagtttt tttaataaca gaaatcctta
    23341 tgcgtcgttt ttgctgaatg cgcaggtaat gctctttttt ctctattata aaaatcttga
    23401 taaaaaacga cgtttctttt atattttact gcaaattccg cttgttttag ctattaactt
    23461 cacttattct aaaacaaatt tattattagc taatatactg cttattattg cttttttatt
    23521 tcatctggtt aggttattag gtcgcaagaa gtttattcgc tttggtattg aagtgttttt
    23581 agcattttta ctcgtaattg gatttattgt ctttcgttat tcgccaaaat tagaaacgac
    23641 gaatatttct ttatttttac atcggacact ccctgatgaa atgtttgaaa gggtgcctgg
    23701 ctcttttgat tcacggaaga aactttggta ttacgcagtt agtcttatgg tcgctaatcc
    23761 gataatattt gtctttggtc acggtccaca tctttcgcgc tatttctatc acttacgaat
    23821 gcaaattgaa atagaaggag taatgacacc gggctttggt gattatcata atggcttagt
    23881 cgaagtaatg catacttttg gcgcaagcgg attaataata tatgcccttg tttttctcct
    23941 tgtaacgata ttactaatta gaagagcaag gacaaatcgc agtttaactt tctttgtctt
    24001 gcttagtatg ggcgtcttta ttttacggag tcaaatggaa tctttagcag ttctcctttt
    24061 taaaagcgaa ggtattatgg cttcactaac attcgtatta cctgcgcttt atttattaag
    24121 aaagtcgcat gaatgtggct ttagtaaaga attaaaagtt aaaaaaacta ggccagaaga
    24181 ggcctagtca tttcttaaaa tatgattaat aagtattatt cggcacggaa gccgtatttt
    24241 ttactcattg cttcggtatg tttaatgtgt tcaatatttg ataacatact tgcggcatca
    24301 gcgtctaaaa taattgttgc ttcgccagca tataactgaa tagcagtaga agtaatttgg
    24361 gcggtgattg gtccttcaag tgctttttgt acaatttctg cttttttagc tccgctagcg
    24421 acaaggatta attttcttgc atctaaaatt gtgccaacg
//
LOCUS       DAOPCD010000002        22544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_2439_length_22544_cov_5.483436, whole genome shotgun sequence.
ACCESSION   DAOPCD010000002 DAOPCD010000000
VERSION     DAOPCD010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 22544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22544
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2439_length_22544_cov_5.483436"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="PLQ55_00120"
     CDS             complement(<1..1387)
                     /locus_tag="PLQ55_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK28381.1"
                     /translation="MLVLKDIYKDYYVAKKPLHVLKGLNLTFPKQQFVTILGPSGCGK
                     TTLLNLIGGLDKYTSGDLIIEGKSTKEFKDRDWDAYRNHRVGFVFQSYNLIPHLSVIE
                     NVEISLTLSGMGSSERREKAKQALLSVDLESELYKRPNQLSGGQLQRVAIARALVNDP
                     SIILADEPTGALDSETSEQVMKILKEISKTRLVVMVTHNEDIAKRYSDRVIRMLDGRI
                     TSDTDEAQSKKKRKVKEPTETEKNKGTSMSFLTALKNSYKNILTKKGRTVFTALAGSL
                     GIIGIGLVAAVSNGFQNYINRLERETMANYPIAVFAQTMDMQAGIKKPDKNTEYPDGE
                     EIVVYNENDTETTRITITNNRITSEFLDLVDGLKEDGLAASTMVNYPTQMQILAKPPT
                     DNARTVQWPYYVSVTPLPFGVFTQLPGDEAYITEFYDVIGKDSKFATERNEIMFVLDK
                     YNRISVQALERM"
     gene            complement(1528..2349)
                     /locus_tag="PLQ55_00125"
     CDS             complement(1528..2349)
                     /locus_tag="PLQ55_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28382.1"
                     /translation="MRKRILVIISLLFSVVLNGCANKEKEKSELEKFLIKYKEVYPDG
                     YNFQTGFYSITSFKKSKVENTDYFHIETYNIKAQLQSKPYGTLWNFEANMMQLNHSLV
                     GGVGTEIPNENFENILWDGEYLHKTIKYNNGYVPKETWTEKTYGTNQIETLQINTEID
                     KIDYIISTLETKLTDLEKHVVNKSNVNFKNNSLIVSTFFSKLETYKIIRIDFDENFVI
                     SRYQISYTDLGRVPDELRLRQITQILKPIDAIEVNAQLEYEEELIVEKDIFTIQI"
     gene            complement(2849..3685)
                     /locus_tag="PLQ55_00130"
     CDS             complement(2849..3685)
                     /locus_tag="PLQ55_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28383.1"
                     /translation="MKYSLIMQKRILIVISLLISVVLNGCTKPQKEQSELEKFLIKYK
                     EVYPDGYNFQTGFYSITSFTKSKVDSSDYFEIETYNIKAQLQSIPYGVLWNFEANMMQ
                     LNRSLVLGAGREGPIENFENILWDGEYLHKTIKCNNYIHKETWTEKTYGTDQVETLLI
                     NTKLYTIEYVIRILKTKLSYYEKYVFDKSNVDFINNSLIVSTFFSEFEIYKIIQIDFD
                     ENFVISRYQFSYIDLGRVPDEFRPRQITQTLKPIDAIEVNAQLEYEEELIVEKDIFTI
                     QI"
     gene            complement(3766..4221)
                     /gene="tadA"
                     /locus_tag="PLQ55_00135"
     CDS             complement(3766..4221)
                     /gene="tadA"
                     /locus_tag="PLQ55_00135"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HPK28384.1"
                     /translation="MNKDEKYMKLALKEAHKAAAIDEVPVGAVIVLNDKVIARGFNKR
                     EINNDPLGHAEMIAIRKASKKLNNWRLVDCELYVTVEPCAMCAGAIMWSRLKRVVYGT
                     GDPKGGAMGEAFNLFDERIVNHIPEIKGGVLAKECKEVIQSFFRNKRKI"
     gene            complement(4226..4322)
                     /gene="ffs"
                     /locus_tag="PLQ55_00140"
     ncRNA           complement(4226..4322)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLQ55_00140"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(4332..5609)
                     /gene="serS"
                     /locus_tag="PLQ55_00145"
     CDS             complement(4332..5609)
                     /gene="serS"
                     /locus_tag="PLQ55_00145"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPK28385.1"
                     /translation="MLDINLIRNEAEKVKSLLFKKGWDFDPEPILSRDEKRRKLRVEV
                     EEAKAKLNKLSASVPVVKKAGGDIQAIFAEVKDLKAENEAKEKELKKLDEEVFNLLAE
                     LPNLPDEDLLAGGKENNKVVYTFGKMPKFTFPIKDHVELCESLGLIDYKRAAKISGSG
                     TWIYTKAGALLEWGLLNFFVSEHLKAGYDMILPPHLLNYESGFTAGQFPKFKEDVFWL
                     EGTEPRKFLLPTSETALVNFYRDEILKEDELPKKYFAYTPCYRSEAGSYRTEERGMIR
                     GYQFNKVEIFQYTKEQDSSKAFEEMVAHAAGLVEKLGLHFQISKLAAGDCSHSMARTY
                     DIEVYIPSMGIYKEVSSVSNARDYQARRGMARYRDKETNAVKYLHTLNGSGLATSRLI
                     PAIVEQFQQADGTVLVPEVLQAFVGTKVLHPDW"
     gene            complement(5865..6866)
                     /locus_tag="PLQ55_00150"
     CDS             complement(5865..6866)
                     /locus_tag="PLQ55_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28386.1"
                     /translation="MKILISFPHKKIDVFFEGAMLEYQIKKACQRDDVTLVTKFSPDI
                     DIANFINLNSQSSKVIRQSIAASIPTLLWMFFANNDTYARVIETRKDGELYIPLSRLE
                     LINMMDGIVVPAQEARVILRKLGVKIPIFVIYGAVDVRRIDELKTSKTDVFRRYFRIN
                     DDQKYAITVMNIKASEHIDQLNDLAAAVPNYNFYVFISGGTSFIDRLRLKAMDKLTSP
                     NLIVTKLVPEDVYRMGVIGASYFIDLGYDKMNVMTLFEPMYLKVPLILRKNVIFKEIV
                     DKTKAYIVNDYNGAAYLIRTEAETDKITEKAYEYTQNYNAALFTEAIYNLFYKIYTR"
     gene            complement(6869..9397)
                     /gene="gyrA"
                     /locus_tag="PLQ55_00155"
     CDS             complement(6869..9397)
                     /gene="gyrA"
                     /locus_tag="PLQ55_00155"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019243409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPK28387.1"
                     /translation="MVEDNIKDMLEEEEKHELEGIDTGIHPVDVVDEVRKSFLDYAMS
                     VIVSRAIPDVRDGFKPVHRRIVYGMYESGMTPDKPFKKSARIVGDVMGKYHPHGDQAI
                     YSTLVRMAQPFSLRYPLVDGHGNFGSIDGDEAAAMRYTEARMEKLATEMVRDINLNTV
                     DFGGNYDGSEKEPEVLPSRFPNLIVNGSDGIAVGMATYMVPHNLSETIDAINLVAKNP
                     DVTPREIFENGMLGPDFPTGASILGRKGILDYFETGEGSIIVRAKTHFETKGDRTWIV
                     IDEMPYKLNKATTIEAIALLVRNKEIDGITDIRDESNKEGIRVVIELRRDVVPEVVLN
                     NLLKQTRLQTSYKITNLCLVNKAPRILNMKELIVEYLNFQVEIIRRRTEHQLSQAEAR
                     DHIVEGLLKATDNIDEIVSIIKNSESGEEASQKLMERFLFSEIQTREVLGMTLRRLTG
                     LEIDKLNKEREQLLANIERYRLILSARDHMVDVVLEELEEIKAKYGDERRTEISDDLA
                     DIDNEDLIPVEDIVVMLTNQGYIKRVDPEEFRTIKRGGVGVKGMRTKEEEIIDIILHT
                     NTHTDVLFFTSKGKVYRKRGYQIPQYRREGKGLPVINLLNIEKEEAVRAIVSTDTYGE
                     DHYLLYVTRNGVTKRTTLAEFSRINVSGKIAIDLREGDELMDVKITDGTAIIGIGSSN
                     GKMASFYETDVRAMGRTAAGVRGIKLDEGAYVVGVTTSLEGALIFALTTKGYGKLTPA
                     NDYRLTKRGSKGVITIKESERTGTLAGIRAIKGDEDLFVTTASGMVVRTNFKEISTTS
                     RNTMGVRVIRLRPDDVVCSFAVVEKGIAFDEDDHEGEGTPSHEE"
     gene            complement(9408..11348)
                     /gene="gyrB"
                     /locus_tag="PLQ55_00160"
     CDS             complement(9408..11348)
                     /gene="gyrB"
                     /locus_tag="PLQ55_00160"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPK28388.1"
                     /translation="MRARESYTAEDIQVLEGLQAVRQRPGMYIGTTGLPGLHHLVWEI
                     VDNAVDEALAGFCTTIDVSINKDGSVTVADNGRGMPVGIVSQTGKTAVETIFTVLHAG
                     GKFGKGAYQVAGGLHGVGAAVVNALSERVEVNVYKDGDEYYLAFNNDGHTEGELKKVG
                     PTNKRGTRVTFKPDPLIFVESVEFDYKTISTRLRQMAYLNRGIRINVEDRRGEELKRE
                     EFYYEGGIAEYVQHINKNRTPITQDVIFVEGKGDVEINGQKSHILVEVAIQYTTEYKE
                     TIFSFANNIQTGDGGTHEQGFRLAVGRVFNNYAKEAKIHKNEDDRFTYEDVVEGMTAI
                     ISVKHPNPQFEGQTKGKLGNTDTRAVVSQVLGEKLEEYLLENPNEAKLILEKISLSSK
                     ARRAATLAREGVRRKDGIEFTTLPGKLADCSSRNPEESELFIVEGNSAGGSAKNGRNR
                     HTQAILPLRGKILNVQKAQETRIYENAEIGNMIQAIGAGIAEEFDITKARYHKIIIMT
                     DADVDGSHIRVLLLTFFNRFMRPLIQNGYVYIAQPPLYKVDYRGKQYYCYNDEQLEKL
                     KVDLDLRPGYPFQRYKGLGEMDAHQLWETTMDPANRKLLRVHIEDAIEADQVFEDLMG
                     EKVEPRKEFINENALFVKNLDV"
     gene            complement(11408..12475)
                     /gene="recF"
                     /locus_tag="PLQ55_00165"
     CDS             complement(11408..12475)
                     /gene="recF"
                     /locus_tag="PLQ55_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HPK28389.1"
                     /translation="MLISDLELLNYRNYPKLNVIFTSGLNVIVGDNGVGKTNVVEAIH
                     LFAFAKSFRTNDTKALIKHDTAKAIVSGTIHIPSRTDISIELTNRSKRILINGKVLPK
                     VSQLSKYVRTTTFEPEDVLFFDASPAKRRKFLDANLSSADEVYLATLVRYEKLLKERN
                     VLLKTDDNDEALDLIDKLFIELAKTIVEKRTRFIRELNKVVNEVLLKLSDEHLSVRLT
                     YESSLGSVQNIERNGLKRLAEVRAKENILESTTLGPHRDDLIATINGHGVKEHASQGQ
                     KRLIVIALALAPYFLEKDLHKKPIIILDDVLSELDKRHQERLIELLLTCTQAFITATH
                     YDNHAHAIYEVTSDRELRRIT"
     gene            complement(12465..12668)
                     /locus_tag="PLQ55_00170"
     CDS             complement(12465..12668)
                     /locus_tag="PLQ55_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HPK28390.1"
                     /translation="METKPYITLQQALKKYNLVSTGGEAKFVIQNGEVKVDGILETRR
                     GRKLYGGEIITYAAQKVVVKDVD"
     gene            complement(12837..13970)
                     /gene="dnaN"
                     /locus_tag="PLQ55_00175"
     CDS             complement(12837..13970)
                     /gene="dnaN"
                     /locus_tag="PLQ55_00175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPK28391.1"
                     /translation="MKFKINREQFLKGLSIASRPTQAKSPIPILGNILLSLDETGLTL
                     LGSSNELAIRTHIPMRVNDLEVIRMFLPGETLISSKTLNESIRKMEGNEIDVEIIDRT
                     IAQISDGRTEYRLGAIDSKDYPDIDLSQDGTVFEIGAKDFIKLVDQTSFAASVREQRP
                     ILTALNLNAVNGTLTAVATDSARLANKVVTLKDNVSFVANVPARIMNEVAKLLETMVG
                     IETIEVSASANKINFMFDDTLVVSRLVAGEYPPTRSIIPKTHASFLEANANELLKAMD
                     RVSILYAAEKDNIVKLSLSPESVVVSARSTFSAESAREEVALCKYVGEPLEISFNYTL
                     VSSAIKATGAEDVTIGFNGGHKPFSIRVATDDSLVLIVTPVRT"
     gene            complement(14099..15454)
                     /gene="dnaA"
                     /locus_tag="PLQ55_00180"
     CDS             complement(14099..15454)
                     /gene="dnaA"
                     /locus_tag="PLQ55_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPK28392.1"
                     /translation="MNMSISEITQIWDRTLIKLQEKIQDRRVYGAFFPDTYIHSINNG
                     IITVIANSSLAKSMLNTNYRDVIEETVGEITQSNYQISFIEKSEIPRVSEIISEKKET
                     FFKNAKVNSNLTFDNFVVGSSSNLEAYQAAVVVANSPGKLYNPLFLYSASGLGKTHLL
                     HAIGNHVKQNSPNLKVLYITADEFTDEFIRFVRDNPGGSLKDFFRTVDVLLMDDVQFL
                     ADKKSTSETFFHLFNLLVGSGKQIVLTSDRHPSELKGIEERLVTRFNSGLQISISAPD
                     LETRKDILRMKISLNNLDIEDFDEEVITFFAENFTNNVRELEGALNRLLFYVVSIAPT
                     KHVTMEVALNSVQSLINVKDAVTKLSETKIINVVADYYNLTPALIIGRNRTSQVALAR
                     HIGMYLTREILDLPFARIGKAFGGKDHSTVINAITKVEKQLKEDPALIQAINEIKERL
                     Q"
     gene            15961..16095
                     /gene="rpmH"
                     /locus_tag="PLQ55_00185"
     CDS             15961..16095
                     /gene="rpmH"
                     /locus_tag="PLQ55_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPK28393.1"
                     /translation="MKRTYQPSKLRRARLHGFRARMATPGGRRVLRNRRAKGRESLTV
                     "
     gene            16119..16442
                     /gene="rnpA"
                     /locus_tag="PLQ55_00190"
     CDS             16119..16442
                     /gene="rnpA"
                     /locus_tag="PLQ55_00190"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPK28394.1"
                     /translation="MKREHRILKNEQFQNIIKNGKLTKTPLCYGYFASNELETLRVGV
                     AVSKKIGNAVTRNKIKRQIRAVVRPYVKTHTGDFIIVAKKEINDSSFGDLVDAIAKIF
                     DSNGD"
     gene            16444..17823
                     /locus_tag="PLQ55_00195"
     CDS             16444..17823
                     /locus_tag="PLQ55_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HPK28395.1"
                     /translation="MKKNKKRIIKLGAIVAGLFVLSSCTASFCSAKDVGYMLYAQDSG
                     LVTEVVEGTETKVHNKVLHKIILDAQSQGYATPSLEYYEKLDQKVLDYAILNFDNSEN
                     LEGAALNAAALKQNGYLKFLGTKGATVVTADGNELWVNWTIWNKEIGAEIGYENVPDR
                     DFANFYKTQIYNKIKANRACIALYDGEYGPEDSKIPVEAKTWKGAWKKGFIEGLIVYP
                     VAALLEFLTKSFGAGGWGQIWAILVTTIIVRGLLVLMTLKQTVGAQKMQALQPELAKI
                     QQKYPNSDTNQYERQALAQAQMALYKKHGINPLGSLLVMFIQFPIFIGVWGAMTGSAS
                     LASDSVLGLNLSAQLGQSMINGWFKGGWWTAWVLFILMTAAQFVSTKLSTWLNKSKTK
                     EIGKTTANPAADKQQRQSKMMMNIMFIMIVFMSFTLPAAMGVYWFVGAIISIVQTVVV
                     HNVMKGRKQ"
     gene            17823..18464
                     /locus_tag="PLQ55_00200"
     CDS             17823..18464
                     /locus_tag="PLQ55_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HPK28396.1"
                     /translation="MRIFEAKNLDEVLALASEDIGIQTKDLVYEVLEEKKGLFKSSIK
                     ISVYETTDYIEYAENYLRLVVESLGFTPEFTTSLDGEVIKIEMSTNHNSILIGKGGKT
                     LLALNEIVQVAMTVKFKKRIRVLLDINNYKNDRYRRVTGLARKVAGDVARSKQDATLD
                     PMPSDERRAIHNALSEYRNVTTESIGAGKERQVIIRYKKPEKKEKAPKEPQDE"
     gene            18533..19855
                     /gene="mnmE"
                     /locus_tag="PLQ55_00205"
     CDS             18533..19855
                     /gene="mnmE"
                     /locus_tag="PLQ55_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPK28397.1"
                     /translation="MLDTIVALATPPMMGALAIIRLSGPEAFNIVTKSFTKDLTKLEK
                     STMVYGKLFDDEELVDEVIAFCFKGPNSFTGEDVVEISCHGSMLIANEIIELMIKNGA
                     RLAGRGEFTNRAYLNGKLDLVQAEAVNDMITATTKEAKKLSLLSLVGETSKIVYPIRE
                     RIADVMSLIEVNIDYPEYRDIEEASYALVISEVDYINAEIKKLIDQGRQGQIIKEGIK
                     VAIVGAPNVGKSSLLNALLKEDKAIVTDIAGTTRDVVEGEINLHGLRLLLLDTAGIRE
                     STDAIESIGIEKTKKSIADADLVIYVKDAENVSKKDEFTGLIKGKKVITVYNKGDLVS
                     KKDPNRLYISALTNDIGALEKAIAKVVGVDEDSYLRPSLNNARQLALLRKAYGELEIA
                     KQEALDEVAIDLVSVSLQQAFFTIKEILGENVDNDLSKEIFSRFCVGK"
     gene            19858..20625
                     /locus_tag="PLQ55_00210"
     CDS             19858..20625
                     /locus_tag="PLQ55_00210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPK28398.1"
                     /translation="MFFDTHCHLNDKRYENDIEGYVERAKAVGVNYLLIVAWDVKSSK
                     KAIKIAERFNNVYAAAGIHPVDAVKTPKSDLKELEKLLTHPKVIAVGEIGLDYHWIKD
                     EKERAIEHEFFIAQIELANKYKKPVLIHMRDATSDTYEVLKSNIPRYQGIMHSYSGSK
                     EMALRFIDLGMYISLAGPVTFLNAREPKEVAKVVSEERLLLETDAPYLTPHPYRGKLN
                     ESANIPLIAKTIATIRNVSVEKVAEFTTKNAKTLFKL"
     gene            20622..21161
                     /locus_tag="PLQ55_00215"
     CDS             20622..21161
                     /locus_tag="PLQ55_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4093 domain-containing protein"
                     /protein_id="HPK28399.1"
                     /translation="MSSKTLLNCIFVAEGKRDEERLKKLDIPYIVRTDGLNVPRETTK
                     HLQELAKIHEVIILTDPDGPGQKIADKLLQEIPNAKVIKVIKKKAVKKGKVGIEHIPL
                     PLLAELIKPYKQETFTTSSDVTLIKLLDLGLSGPNSQFKKIKLATKYSLLVASLKNML
                     TQIQLLNISYKELKEALSE"
     gene            21154..21939
                     /gene="rsmA"
                     /locus_tag="PLQ55_00220"
     CDS             21154..21939
                     /gene="rsmA"
                     /locus_tag="PLQ55_00220"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPK28400.1"
                     /translation="MNKEEIITLLAKENLSPQKRKGQNFLVDENVARKIVSFLEPNYI
                     DVIEVGPGLGALTKELLLKTVNFTAIEIDKGFVRVLRAKYPHLNIIEKDFLEYSVPRE
                     TKAVISNIPYYITTKIIEKVLLEAENLKKFVFMTQIDVKERLLAKSGTKAYSPLRVLL
                     SLLGDVEQKLVVTADKFYPVPHVDSAVFCFTRYKTDIPVREFYSFLNNAFLNRRKTLY
                     NNLKNIYDKEDILTALKKHRINENVRAEALSPNDLLTLFETLR"
     gene            22242..>22544
                     /locus_tag="PLQ55_00225"
     CDS             22242..>22544
                     /locus_tag="PLQ55_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28401.1"
                     /translation="MKNKKKLFWLILPFTLIMFALLFALYPTPWGAKEPYKVFHSPEK
                     HLKEVEKRVNQKIEEREMDYTNLRALPLYDEYNKFSFVLVEFEPQDFMILKVYRTSI"
BASE COUNT         6905 a         4223 c         3916 g         7500 t
ORIGIN      
        1 ccatccgttc taaagcttgg actgagatac gattatattt atcaagtaca aacataattt
       61 cattgcgctc tgtcgcaaac tttgagtctt taccaattac atcataaaat tcagtaatat
      121 aggcttcgtc ccctggtaat tgggtaaata ccccaaaggg tagtggcgtc acactgacat
      181 aataaggcca ctgtacagta cgcgcgttat cagtgggcgg ctttgctaaa atttgcattt
      241 gtgttggata attaaccatt gtactagcgg caagcccgtc ttcctttaat ccgtccacta
      301 aatctaagaa ttcagatgtt atcctattat ttgtaatggt aatccgtgtt gtttctgtat
      361 cattttcatt ataaacaacg atttcttcac cgtctggata ctcagtgttt ttatcaggtt
      421 tttttatgcc tgcttgcata tccattgttt gcgcaaaaac ggcaattggg taatttgcca
      481 ttgtttcccg ctccaaacga ttaatataat tttgaaatcc gttactaacc gctgctacta
      541 acccaatacc aataatccct aatgatcctg caagcgcagt aaacactgtt cttccttttt
      601 ttgttaagat gtttttataa ctgtttttaa gcgcggttaa aaaggacatt gatgttccct
      661 tgtttttttc tgtttccgtt ggttctttta ctttgcgctt ctttttactt tgtgcttcat
      721 cagtgtcact agtaattctg ccatctaaca tcctaattac tcgatcgcta tatcgtttag
      781 cgatgtcttc attgtgcgta accattacaa caagtcgtgt tttactaatt tcttttaaaa
      841 ttttcatgac ttgttcgctt gtttcactat ctaacgctcc ggtgggttca tccgctaaaa
      901 tgatggaagg atcattaaca agtgcacgtg caatcgctac tctttgaagt tgaccaccag
      961 aaagttgatt tggtctttta tataattcac tttcaagatc aacactaagg agggcttgtt
     1021 tagccttttc gcgacgttca ctactcccca tcccagaaag cgttaaagaa atctcaacat
     1081 tttcaatcac acttaagtgc gggataagat tataactttg aaacacaaat ccaacacgat
     1141 gattacgata agcgtcccaa tctctatctt taaattcttt tgttgattta ccttcaatga
     1201 ttaaatcgcc tgatgtatat ttatcaagac cgccaatcaa gtttaaaagt gttgttttac
     1261 cacaacccga tggacctaaa attgttacga attgttgttt aggaaaagta aggtttagcc
     1321 cctttaaaac atgaagtggc tttttagcca cataataatc tttataaata tctttgagaa
     1381 caagcataaa tatccccttt cggatttcac tttatacatt gtctaacttt ttatttatct
     1441 tttcaagata aaggcattat tatttacatt aattttataa ttgcggtttt acaagagacg
     1501 ggaatttagt taatctttgt tactatacta aatttgaatc gtaaatatat ctttttccac
     1561 gataagttct tcttcatatt ctaattgagc gttaacttca attgcatcta ttggtttcag
     1621 tatttgtgtt atttgtctta gtcttaattc atcaggaact ctacctaagt cagtatatga
     1681 aatttgatat cgtgaaatta caaaattttc atcgaaatca attcgtataa ttttatatgt
     1741 ttctaattta ctgaaaaatg tagaaacaat caacgaattg tttttaaaat taacatttga
     1801 tttattaaca acatgttttt ctaaatcagt cagcttagtc tcaagagtac ttataatata
     1861 atcaatttta tctatttcag tatttatttg taatgtctct atttgatttg tgccatatgt
     1921 tttttcagtc catgtttctt taggtacata accgttgtta tattttattg ttttatgcaa
     1981 atattctccg tcccataata tgttttcaaa attttcgtta ggaatttctg ttcctactcc
     2041 gcccactaaa gaatgattta actgcatcat atttgcttca aaattccata atgtaccgta
     2101 tggttttgat tgcagttgag ccttaatatt atatgtttct atatgaaaat aatctgtgtt
     2161 ttcaaccttt gattttttaa atgatgttat actataaaat cctgtttgaa agttatatcc
     2221 gtctggatac acttctttat atttaatcag gaatttttct aattcgcttt tttctttttc
     2281 tttatttgca caaccgttaa gaacaacact aaaaagtaat gaaattataa caagaattct
     2341 ttttcgcata agaattttct tacctcctca tccttgggaa tcctgacggc attggcccac
     2401 cttttttata tccctgcatt tgtttcattt gttcaagcgt atctatcatt taaatcaaaa
     2461 tctccttccg taatcttgcg cgttgttttt tgtcttttgt tcaaattgtt gttgtgcttt
     2521 ttcaaccaat gttaatacaa cctcttttcc tgcgttgtgt caaataatat aaaacatgac
     2581 aataaattgc acattaatct tagtttttaa atattctaaa ttagttttac taaatcttat
     2641 caacataaat tctggtggtt ccaacgctaa ttcttccttt atttctactg ccgactgctt
     2701 cagaagtcat ataatatcta aaacatttag taccgttagt ggggaagttg ataatatatg
     2761 tcttcagttt taatgtttcc aaatgataaa catgtcgtta atactagtcc caaagttgta
     2821 agcatatgat taatctcctt tgctaaaact aaatttgaat cgtaaatata tctttttcca
     2881 cgataagttc ttcttcatat tctaattgag cgttaacttc aattgcatct atcggtttca
     2941 gcgtttgtgt tatttgtctt ggtctaaatt catcaggaac tctacctaag tcaatatatg
     3001 aaaattgata tcgtgaaatt acaaaatttt catcgaaatc aatttgtata attttatata
     3061 tttcaaattc actgaaaaac gtagaaacaa tcaacgaatt gtttataaaa tcaacatttg
     3121 atttatcaaa aacatatttt tcataataag acagcttagt cttaagaata cgtataacat
     3181 attctattgt atataattta gtatttatta gtaatgtctc tacttgatct gtgccatatg
     3241 ttttttcagt ccatgtttct ttatgtatat aattgttgca ttttattgtt ttatgcaaat
     3301 attctccgtc ccataatatg ttttcaaaat tttcaatagg gccttctctt cctgcaccca
     3361 acactaaaga acgattcaac tgcatcatat ttgcttcaaa attccataat acaccgtacg
     3421 gtattgattg cagttgagcc ttaatgttat atgtttcaat ttcaaaataa tcagagctat
     3481 caaccttcga ttttgtaaat gatgtaatac tataaaatcc tgtttgaaaa ttatatccgt
     3541 ctggatacac ttctttatat ttaattagga atttttctag ttcactttgt tctttttgtg
     3601 gttttgtaca gccgttaaga acaacactaa ttagtaagga aattacaata aggattcttt
     3661 tttgcataat taaactatat ttcaaaacta ctcctgtgtc aaataataga aagcgcacat
     3721 tatgattatt caattatcgt atgtaaacat ttatttatat acatattata tttttcgttt
     3781 gttacggaaa aatgattgaa tcacttcctt acactcttta gcgagcaccc cgccttttat
     3841 ttccggtata tggttaacta ttcgctcatc aaaaagatta aacgcttcac ccatggcgcc
     3901 gccttttgga tcaccagtgc cgtacactac ccgttttaag cgactccaca ttatcgcgcc
     3961 cgcacacatt gcgcacggtt caactgtgac atagagttca cagtcgacaa gtcgccaatt
     4021 gttgagtttt ttactcgctt tacgaatcgc aatcatttcc gcgtgaccaa gcgggtcgtt
     4081 attaatttcg cgtttattaa aacctcgggc aataacttta tcgttaagca cgataactgc
     4141 accaacaggt acttcatcaa ttgccgctgc tttgtgcgct tctttaagcg ctagcttcat
     4201 atatttttcg tctttattca tagtttaaat taaaaaccac cgcacatgat taagtaatct
     4261 taaggctgct acgttcccgt cctgacctgg ttcgaagaat aaccatcgcg atggcacctt
     4321 cattatagca actaccagtc aggatgtaaa acttttgtcc ccacaaacgc ttgtaaaact
     4381 tcaggaacta aaacagtgcc gtctgcttgt tggaattgtt caacaattgc tggtattaat
     4441 cttgatgttg ctagtccaga accgttaagt gtatgtaaat atttcaccgc atttgtttct
     4501 ttatcgcgat aacgcgccat accgcgccta gcttgataat ctctagcgtt actaacactg
     4561 ctaacttctt tataaatacc cattgacgga atatatactt caatatcata agttcttgcc
     4621 attgaatgcg aacagtcacc tgccgcaagt ttactaattt ggaagtgtaa gcctaacttc
     4681 tcgacaagac ctgctgcgtg agcgaccatt tcttcgaaag cttttgatga atcttgctct
     4741 ttagtgtatt ggaatatttc aactttatta aattgataac cacgaatcat cccgcgttct
     4801 tcagtgcgat aactacctgc ttcgctacga taacacggag tgtaggcaaa gtatttcttt
     4861 ggtaattcat cttcttttaa tatttcgtca cgataaaaat taacgagtgc tgtttccgaa
     4921 gttggtagta agaatttacg cggttctgtt ccttctaacc aaaacacatc ttctttaaat
     4981 ttaggaaatt gtccagcggt aaagccactt tcataattta ataagtgcgg aggtaaaatc
     5041 atgtcgtacc ctgcttttaa gtgttcacta acaaaaaagt ttaaaagtcc ccattcaagt
     5101 agtgctcctg ctttagtgta aatccacgtg ccgcttcctg aaatttttgc agctctttta
     5161 taatcgataa gtcctaaact ttcacaaagt tcaacatgat ctttaatcgg aaaagtaaac
     5221 tttggcattt taccaaaagt atacacaact ttattatttt cttttccacc cgcaagtaag
     5281 tcttcgtctg gtaaatttgg taattctgct agtaaattaa aaacttcttc atcaagtttc
     5341 tttaattctt tttccttagc ttcgttttcc gctttcaggt cttttacctc agcaaaaatg
     5401 gcctgaatgt cgcctccagc ctttttaaca accggtacac ttgcgcttaa tttgttgagt
     5461 tttgctttag cttcttcaac ttcaacgcga agtttccgcc gtttttcatc acgacttaaa
     5521 attggttcag gatcaaaatc ccaccctttt ttaaaaagta aacttttaac tttttctgct
     5581 tcatttctaa ttaaattaat atctaacata atacgcactc ctttcttaaa caaaataaaa
     5641 cgccctaatt aggacgtctt taacgtggtg ccacctaagt ttgatacaaa gttgtattct
     5701 cattattgtc ttaacgcgac aaacgaagcg attaagcttt gttctccgag gtggaattca
     5761 taacctttag ttctcgcctt cccactatca gcgagtcact gttaaaccgc gaattattac
     5821 tagtctcttc actgaacaca tttattttat caagttttac tttattagcg agtatatatc
     5881 ttataaaaca agttataaat cgcttctgta aataaagcgg cgttataatt ttgtgtatat
     5941 tcgtaagctt tttccgtaat tttatctgtt tctgcttctg ttctaattaa gtaagccgct
     6001 ccgttatagt catttacaat gtaagctttt gtcttatcaa caatttcttt aaaaattaca
     6061 tttttccgta aaataagtgg tacttttaag tacatcggct caaaaagcgt cattacattc
     6121 atcttgtcgt aacctaaatc aatgaagtaa cttgcaccaa taacacccat ccgataaaca
     6181 tcttctggta caagcttagt cacaattaag ttcggtgaag ttaatttatc catcgcttta
     6241 agccgcaatc tatcaataaa tgatgtgcca ccgctgatga aaacatagaa attatagttt
     6301 ggtacagcag cggctaagtc attaagttgg tcaatatgtt cacttgcttt aatgttcatc
     6361 actgttatgg catatttttg atcatcgttt atacggaaat agcgacgaaa tacatctgtt
     6421 ttgcttgttt ttaactcatc aatccgtcga acatcaaccg ctccataaat tacaaagatt
     6481 ggaattttta caccgagttt tcgtaaaata acacgcgctt cttgtgctgg aacaacaata
     6541 ccgtccatca tattaattaa ttcaagacgc gataaaggaa tgtataactc tccatctttt
     6601 cttgtttcaa tcacacgagc gtaagtgtcg ttattagcga agaacatcca taaaagcgtg
     6661 ggtatacttg cggcaattga ttgcctaatc actttcgaag attgactatt caaatttata
     6721 aaattagcga tatcaatatc aggactaaat ttagtaacaa gtgttacatc atctctttgg
     6781 catgcttttt ttatttgata ttcaagcatt gctccttcaa agaagacatc aatcttttta
     6841 tgtggaaaag agattaatat tttcataact attcttcgtg actaggagtc ccttcacctt
     6901 cgtgatcatc ttcgtcaaaa gcaatccctt tttcgacaac tgcgaaagaa caaacaacat
     6961 catctgggcg taaacgaatg acacgcacac ccattgtatt tcggcttgtt gtgctaattt
     7021 ctttaaagtt ggtgcgcaca accattccgc tagcggtcgt aacaaataag tcttcatctc
     7081 ccttaattgc tctaattccc gcaagtgttc cagtacgttc actttcttta atggtaatta
     7141 caccttttga acctcgtttt gttaaacggt aatcgttagc aggtgttaat ttaccataac
     7201 cttttgttgt aagcgcaaag attaaagcac cctctaaact tgttgtaacg ccgacaacat
     7261 aagcaccttc gtcaagttta ataccacgga ctccggccgc agttctgccc attgcccgca
     7321 catccgtttc atagaaactt gccattttac cattacttga accgatacca attattgccg
     7381 taccatcagt aattttgaca tccattaatt catcgccttc gcgtaaatca atagcgattt
     7441 taccactgac attaatgcgt gagaattccg ctaaagttgt acgttttgtc acaccgttgc
     7501 gggttacata taagaggtaa tggtcttcac catatgtatc agtcgacaca atagcacgta
     7561 ccgcttcttc tttttcaata tttaaaagat taatgactgg taagcctttc ccttcacgac
     7621 gatattgcgg gatttgataa ccgcgtttac gatatacttt ccctttgctt gtaaagaata
     7681 agacatcagt gtgtgtattt gtgtgtaaga taatgtcaat tatttcttct tcttttgtgc
     7741 gcattccttt tacaccaacg ccaccgcgtt taatcgttct aaattcttca ggatcaacgc
     7801 gtttaatata gccttggttt gtgagcatca caacgatatc ttcaactgga attaagtctt
     7861 cgttatcaat atcagcaagg tcatcactaa tttcagtacg gcgttcatcg ccatacttag
     7921 cttttatttc ttctaactct tcaaggacaa catcaaccat atggtcacgt gcacttaaaa
     7981 ttaagcgata gcgttcaatg ttcgcaagta gttgttcgcg ttctttattt aatttgtcaa
     8041 tttctaaacc agttaagcga cggagtgtca ttcctaaaac ttcgcgtgtt tggatttcgc
     8101 taaagaggaa gcgttccata agcttttgac tcgcttcttc accagattca ctatttttaa
     8161 taattgatac aatttcatca atgttatcag tggcttttag taatccttcc acaatgtggt
     8221 cgcgcgcttc tgcttgcgaa agttgatgtt ctgtacgacg acgaataatt tccacttgga
     8281 agtttaaata ttcaacaatt aattccttca tgtttaaaat acgcggagct ttattaacta
     8341 aacataaatt cgttatttta tatgaagttt gtaaacgtgt ttgttttaat aaattgttta
     8401 acacgacttc tggtacgaca tcacgacgta attcgataac cacacgaatt ccttctttgt
     8461 ttgattcgtc acgaatatca gtaatgccgt caatttcttt attacgcact aaaagggcaa
     8521 ttgcttcaat tgttgttgct ttatttaatt tataaggcat ttcatcaatg acgatccatg
     8581 tccgatcacc ttttgtttca aagtgcgtct tagcgcgaac aatgatactg ccttcaccag
     8641 tttcaaaata atctaaaatc cctttgcgac ctaaaatgct cgcaccagtt gggaagtctg
     8701 gacctagcat tccgttttca aaaatttcgc gtggtgtaac atcaggattt ttcgctacta
     8761 ggttaatcgc atcaattgtt tcacttaaat tgtgcggcac catatatgtt gccatcccaa
     8821 cagcaatccc gtcactacca ttaacaatta agtttgggaa gcgtgatggt aatacttctg
     8881 gttctttttc actaccatca tagttaccac caaaatcaac agtgtttaaa ttaatatcac
     8941 gcaccatttc cgttgcaagt ttttccatcc gtgcttcggt ataacgcatc gctgctgctt
     9001 cgtcaccatc aatactaccg aagttaccgt gtccgtccac aagcggataa cgtaaactaa
     9061 atggttgcgc catcctgact aatgttgaat aaattgcttg gtcaccatga gggtgatatt
     9121 tacccatgac atccccaaca atccgtgcgc ttttcttaaa cggtttgtct ggcgtcattc
     9181 ctgattcata catcccgtaa actatccgac gatgtactgg tttgaacccg tctctaacat
     9241 ctggaatagc gcgactaaca attaccgaca ttgcataatc taaaaatgat ttacgaactt
     9301 catctacgac atcaaccggg tgtataccgg tgtcaatgcc ttctaattca tgcttctctt
     9361 cttcttcgag catatctttg atattgtctt ctaccatgat gtcctcctta tacgtcaagg
     9421 tttttcacaa agagggcgtt ttcattaata aactctttgc gtggttcaac cttttctccc
     9481 attaaatctt caaacacttg atcagcttcg attgcgtctt caatgtgtac tcttaaaagt
     9541 tttctgtttg cgggatccat tgttgtttcc cataattgat gtgcgtccat ttcccctaac
     9601 cctttatagc gttggaaagg ataacctgga cggagatcta aatcaacttt aagcttttct
     9661 aattgttcgt cgttataaca ataatattgt ttaccgcgat aatctacttt ataaagcggt
     9721 ggttgggcaa tgtatacata accattttga ataagtggac gcataaaacg attaaagaat
     9781 gttaatagta atacgcgaat atgactacca tcaacgtccg cgtctgtcat aataataatt
     9841 ttatgatacc gtgctttagt tatgtcgaat tcttcggcaa ttcctgcacc aattgcttga
     9901 atcatgttcc caatttcagc gttttcatag attcttgttt cttgcgcttt ttgcacattt
     9961 aagatttttc cgcgtaacgg caagatcgct tgtgtatgac ggttgcgacc gtttttagca
    10021 ctaccgcccg cactattacc ttcaacgata aatagttcgc tttcttcagg attacgacta
    10081 ctacaatctg ctagttttcc tggcaaggtt gtaaattcaa tcccgtcttt acgacgcaca
    10141 ccttcacgcg ctaatgttgc agcgcggcgc gctttagatg aaagtgaaat cttctctaaa
    10201 ataagtttcg cttcatttgg attttcaagc aagtactctt cgagtttttc ccctaacact
    10261 tgactgacaa cagcccgagt atcagtgtta ccgagctttc cttttgtttg tccttcaaat
    10321 tgtgggtttg ggtgtttaac ggaaataatc gccgtcattc cttcaacgac gtcttcataa
    10381 gtaaaacggt cgtcttcatt tttatgaatt tttgcttctt tcgcataatt gttaaataca
    10441 cggccgaccg caagacggaa accttgttca tgtgttccgc cgtccccagt ttgaatgtta
    10501 ttagcgaacg agaaaatcgt ttctttatat tcagtcgtgt actgaatcgc tacttccact
    10561 aagatgtggc ttttttgtcc gttaatttcg acatcacctt tgccctcaac aaaaataaca
    10621 tcttgagtaa tcggtgttct atttttatta atgtgttgga cgtattctgc aataccgcct
    10681 tcgtaataaa attcttcgcg tttaagctct tcgccacggc gatcttcaac atttattcta
    10741 atcccgcgat ttaaataagc catttgtctt agtcgcgtgc tgattgtttt gtaatcaaat
    10801 tccactgatt cgacaaagat aagtggatca ggtttaaatg ttactcttgt accgcgtttg
    10861 tttgtgggac caaccttttt taattcgcct tcagtgtgac catcgttatt aaacgctaag
    10921 tagtattcat ctccgtcttt atagacatta acttcaactc tttcactaag ggcattaacg
    10981 actgccgcac caacaccgtg gagaccacct gctacttggt aagcgccttt cccgaattta
    11041 ccgccagcgt gtaatacagt aaagatagtt tctacagctg tttttccagt ttgtgagaca
    11101 ataccaaccg gcattccgcg gccgttatcc gctactgtta ccgaaccgtc tttgttaatt
    11161 gagacatcaa ttgtggtaca aaagcctgct agtgcttcgt caacagcgtt atcaacgatt
    11221 tcccacacta aatggtggag tccaggaagt cctgttgtcc caatgtacat cccgggtctt
    11281 tgtcgtacag cttgcaaccc ttctaatact tgaatgtctt cagcagtgta actttcacga
    11341 gcgcgcattt cttgtttagc ttgctctagt tcttcatcta ctattttatg ttttttctct
    11401 tcactcatta ggtaatcctc cttagttctc ggtcacttgt aacttcataa attgcatgag
    11461 cgtgattgtc ataatgtgtt gcggtaataa atgcttgagt gcatgtgagt aataactcta
    11521 ttaatcgttc ttgatgacgc ttatctaatt cacttaagac atcatcaaga ataataatcg
    11581 gttttttgtg taagtccttc tctaagaagt aaggcgctag ggctaaggcg ataacaatta
    11641 atcttttttg gccttgtgat gcgtgttctt ttacaccgtg accattaatt gttgctatta
    11701 agtcgtcacg atgcggtcct agtgttgtac tttcgagaat gttttctttt gctctaactt
    11761 ctgctagtcg ttttaatccg tttctctcaa tgttttggac actaccaagc gaagattcat
    11821 atgttagcct tacgcttagg tgttcatcac ttaacttcaa gagtacttca ttaactactt
    11881 tgtttaactc acggatgaac cttgtgcgtt tttctacaat cgtttttgct agttcgatga
    11941 atagtttatc gattaagtcg agtgcttcat cgttatcatc cgtttttagg agcacgtttc
    12001 tttctttaag caacttttca taacgcacta atgtcgcaag ataaacttca tcagcgctac
    12061 ttaagtttgc gtctaagaac tttcgtcttt tagcgggact tgcgtcgaag aagagtacat
    12121 cttctggttc gaaggttgtc gtccgaacat acttacttaa ttgcgacacc ttcgggagca
    12181 ctttcccatt tattagtatc cttttgctgc ggtttgttaa ctcaatactt atatctgtcc
    12241 gacttggtat atgaattgtt ccgcttacaa tcgcttttgc ggtatcgtgc tttattagtg
    12301 cttttgtatc gtttgttcga aagcttttag cgaacgcaaa gagatgaatc gcttctacaa
    12361 catttgtctt accgacaccg ttatcaccaa cgatgacgtt tagtccactg gtgaagatga
    12421 cattaagctt tgggtagttg cgatagttta atagttcaag atcgctaatc aacatctttc
    12481 actactacct tttgtgctgc gtaagttatt atctctccgc cataaagctt acgaccccgt
    12541 ctagtttcaa ggattccgtc aacttttact tcgccgtttt gaattacaaa cttcgcttct
    12601 ccgcctgttg aaacgagatt atatttcttt agtgcttgct gtaaagttat atatggtttt
    12661 gtttccactt atacgctctc cttgtcgcta tgaacgtatt ataccacaaa aaagaaaagg
    12721 gaatataaat attcccttaa atttgctcct ttttgtgtcc ttagaacgac ttttgcttga
    12781 atcgtgagga tttgcccgtt ttttattaat tttcgctcta aattgctcta ttttaattat
    12841 gtccgaactg gtgtaacaat taacacaagc gagtcatctg tcgctactct gatgctaaat
    12901 ggtttgtgac cgccgttaaa cccgatggtg acatcttcag caccggtcgc tttaattgca
    12961 gagcttacta atgtatagtt aaatgaaatt tcaagtggtt caccgacata cttacaaagc
    13021 gctacttctt cgcgtgcact ttccgcgctg aatgttgaac gcgcagagac aacaacactt
    13081 tctggactta atgacagttt aacgatatta tctttttccg ctgcgtaaag aattgaaaca
    13141 cggtccatcg cttttaagag ttcattagcg ttagcttcta ggaaactagc gtgtgttttt
    13201 gggataatgc tacgagttgg tggatattca ccagcgacaa gccgcgagac aacgagtgta
    13261 tcatcaaaca tgaaattaat tttattggcg gacgcactaa cttcgattgt ttcaatgcca
    13321 accattgttt caagaagctt agcgacttcg ttcataattc ttgctggaac attagcgaca
    13381 aacgaaacat tgtcttttaa tgtcacaact ttgtttgcta gtcttgcact gtctgttgca
    13441 actgccgtta aagtaccatt aactgcattt aaatttaatg cggttaagat tggcctttgt
    13501 tctctcacac tagcggcaaa tgaagtttga tcaacgagtt taataaaatc tttagcgcca
    13561 atttcaaata cggtgccatc ttgacttaaa tcaatatctg gatagtcttt agaatcaatc
    13621 gcgccgagtc gatattctgt gcgaccgtca ctaatttgtg cgatggtacg gtcaataatc
    13681 tcgacatcaa tttcgttccc ttccattttt cttattgatt cattaagtgt tttactggaa
    13741 ataagtgttt cacctggtaa gaacatccga atgacttcga gatcattaac tcgcattgga
    13801 atatgcgtgc ggatcgctag ttcgttgctt gaaccaagta atgttaatcc agtttcatct
    13861 aatgacaata aaatgtttcc taaaatggga ataggagact ttgcttgagt tggtctagag
    13921 gctatcgata aaccttttag aaattgttct cgattgatct taaacttcat aataatattt
    13981 aattcctttc atatttaatg ataataataa gggctgtgga gactgtggat aactccacaa
    14041 aaaagcttat tgactctttt taattttagc acagaagcaa atactttatt atataaatct
    14101 attgtaaacg ctcttttatt tcgtttatag cttgaattaa agcaggatct tctttcaact
    14161 gtttttccac ttttgtaata gcgttaatca cagtactatg atcttttccc ccaaacgctt
    14221 tgccgatgcg cgcgaacgga aggtctaaaa tctcgcgagt taagtacatt ccgatatggc
    14281 gagcaagcgc aacttggctt gtgcgattac gaccaataat aagcgctgga gtaaggttat
    14341 agtagtcagc gacaacatta ataatttttg tttcacttaa tttcgtgaca gcgtctttaa
    14401 cattgattaa agactgcaca ctattgagtg ctacttccat cgtaacgtgt tttgttgggg
    14461 cgatgcttac tacataaaat agtaaacgat tgagcgcacc ttcgagttca cgcacattat
    14521 ttgtaaagtt ttcggcaaag aaagtaataa cttcttcatc aaaatcttca atatctaaat
    14581 tatttaagga tattttcatt cgtaaaatat cttttcttgt ttctaagtca ggagcggaga
    14641 tactaatttg taaaccagag ttaaagcgtg tcactaaacg ctcttcaata cctttaagtt
    14701 cgcttgggtg acggtcgcta gttaagacaa tttgtttacc agagccgacc aataagttaa
    14761 aaagatggaa aaatgtttca cttgtcgact ttttatccgc taaaaattgg acatcatcca
    14821 ttaataaaac atcaacagta cggaaaaagt ctttgagcga accgcccgga ttatcacgaa
    14881 caaaacgaat aaattcatca gtaaactcat cagcggtaat gtataaaacc ttaagatttg
    14941 gggaattttg cttaacatga ttaccaatcg catgtaaaag gtgcgttttt cctaaacccg
    15001 aggcactata aaggaaaagc gggttatata atttacctgg tgaattagcg acaacaaccg
    15061 ctgcttgata agcttcgagg ttgcttgagc ttccgacaac aaaattatca aaagtaagat
    15121 tagaattaac tttcgcgttt ttaaaaaatg tctctttctt ttcgctaata atttcactaa
    15181 cacgcgggat ttcgcttttt tcaataaagc ttatttgata attagattgc gtaatttcac
    15241 caacagtttc ttctataaca tcgcgataat tcgtgtttaa cattgactta gctaagctag
    15301 aattagcgat aactgtaatg attccgttgt taattgaatg aatataagtg tcagggaaga
    15361 aggctccata aactcttcga tcttgaattt tttcttggag cttgataagc gtacgatccc
    15421 aaatttgggt gatttctgaa attgacatgt tcataaacta tttgcctccg caatctttag
    15481 caataattta acataattgg gataaaaata aagagataag ggaataagat ttttttgtcc
    15541 acaatttgtt acttataaaa gcgcgattat atgtagttat ccaccaaatc cacaaaataa
    15601 tggtgtggat aacattgtgg aaaacctttt ccatgttgat aatgtggact tgcgcaagag
    15661 ttatacacag tgttttaaaa ttgtccacaa tgtttttgtt tcatataatc ggtttttttg
    15721 attaattaaa cataatcgta tgtgttttcc acataaaagt ggatattttt caattaatta
    15781 ataaaaaatg atgaaaaaaa gccaaaaaga ccgacagatt taacgatatc ctgttattat
    15841 tcgttgttga aagaagcatc tactaattcg tgtttaatag tcattggttg acttttacta
    15901 tgtaaaccac taaaattaat cgcgaacaac tttatgttgg attgaatgag gagaaatatt
    15961 atgaaacgta cgtatcaacc aagtaaatta agacgggctc gtcttcacgg gttccgcgct
    16021 agaatggcga ctccaggtgg tcgcagagtt cttcgtaacc gacgtgctaa aggtcgtgaa
    16081 tctttaacgg tctaagctga ctacttttga ggagagttgt gaaacgggag catcggattt
    16141 taaaaaacga gcaattccaa aacattataa aaaatggaaa gctcacaaaa accccgcttt
    16201 gctatggtta ctttgcttct aacgaactcg aaactttacg tgtaggtgtc gctgttagca
    16261 agaaaattgg taacgccgtc acccgcaata aaataaaaag acaaataaga gcagttgtta
    16321 gaccgtatgt taaaacgcat actggtgact ttatcattgt ggcgaaaaag gaaattaatg
    16381 attcatcctt cggtgattta gtagatgcaa ttgcgaaaat attcgacagt aacggagatt
    16441 aacatgaaaa agaataaaaa acggattata aaactaggcg caattgtcgc cggacttttc
    16501 gtgctttctt catgtaccgc gagcttctgt tccgcgaaag acgtgggcta tatgctttat
    16561 gctcaagatt cagggttagt tacagaagtc gttgaaggta ctgaaacaaa agtacacaac
    16621 aaagttttac ataagattat tttagatgct caaagtcaag gctacgctac tcctagccta
    16681 gagtattatg aaaagttaga tcaaaaagtt ttagattatg caattctaaa ttttgataat
    16741 agtgaaaact tagaaggcgc cgcacttaac gctgcggcct taaaacaaaa tggttactta
    16801 aagtttttag gtaccaaagg cgcaacagta gtgactgctg atggtaatga actgtgggtt
    16861 aactggacaa tttggaacaa agaaattggt gcagaaattg gttatgaaaa tgttcccgac
    16921 cgcgactttg ctaattttta taaaacacaa atttataaca aaattaaggc aaatcgtgct
    16981 tgtattgcgc tttatgacgg cgaatatgga ccagaagata gtaaaattcc agtcgaagcc
    17041 aagacatgga aaggtgcatg gaagaaaggg ttcattgaag gcttaattgt ttatcctgtt
    17101 gcagcactac ttgaattttt aacaaaatca tttggcgctg gtggatgggg acaaatttgg
    17161 gccatcttag ttacaacaat tattgtgcgg ggactacttg tccttatgac acttaaacaa
    17221 acggtgggtg cacaaaagat gcaagcctta caaccagagc ttgcaaagat tcaacaaaag
    17281 tatccaaact ctgacactaa tcaatatgaa agacaagctt tagcgcaagc acaaatggcg
    17341 ttatataaga aacacggaat caacccactt ggatcactac ttgtaatgtt cattcagttc
    17401 ccaatcttta ttggggtatg gggcgcaatg actggttctg ctagtttagc aagtgatagt
    17461 gttttagggc ttaacttaag tgctcaactt ggtcaatcaa tgattaatgg ttggtttaag
    17521 ggtggctggt ggacagcgtg ggtgttattt atattaatga ccgctgcaca atttgttagc
    17581 acaaaattat caacatggct taacaagagc aaaacaaaag aaattggtaa aacaaccgct
    17641 aatccggctg cggacaaaca acaacggcaa tcaaaaatga tgatgaatat tatgtttatt
    17701 atgattgtct ttatgtcgtt tactcttcct gcagcgatgg gtgtgtattg gtttgtaggg
    17761 gcaattatct caattgttca aactgtagtt gttcataatg tgatgaaagg aaggaaacaa
    17821 taatgcggat atttgaagca aaaaaccttg atgaagtctt agcgctcgca agtgaagata
    17881 ttggtattca aacaaaagac ttagtgtatg aagtacttga agaaaagaaa ggacttttca
    17941 aaagttcaat caaaatcagt gtatacgaaa caactgatta cattgaatac gctgaaaatt
    18001 atttacggtt agtcgtggag tcattaggtt ttactcctga attcaccacg agtttagatg
    18061 gcgaagtcat taaaattgaa atgagcacaa accacaattc aattcttatt ggtaagggtg
    18121 gtaaaacatt actcgcgtta aacgaaattg tccaagtcgc gatgaccgtt aaatttaaaa
    18181 aacgcattcg agtattatta gatattaata attataaaaa tgaccgttac cgtagagtta
    18241 ctggcttagc acgtaaagtt gcgggtgacg tcgcacgttc taagcaagac gcaacacttg
    18301 atccaatgcc aagcgacgaa aggcgcgcga ttcataatgc attaagtgaa tatcgcaatg
    18361 ttaccactga atcaattggt gcaggcaaag aaagacaagt catcattcgt tataaaaagc
    18421 cagagaaaaa agaaaaggca ccaaaagaac ctcaagacga ataaaacaag ataaacacca
    18481 ccgctaaggt ggttttttct tattagataa taggatatga taaaatttaa ctatgttaga
    18541 tacgattgtt gctcttgcta ctccaccaat gatgggcgcc cttgccatca tccgtctctc
    18601 aggacctgag gcttttaata ttgttaccaa aagttttacg aaagatttaa ctaaactaga
    18661 aaaaagcacg atggtgtacg gcaaactttt tgatgacgaa gaacttgttg acgaagtaat
    18721 tgctttttgt tttaaagggc cgaattcttt tactggtgaa gacgttgtcg agattagttg
    18781 tcacggcagc atgcttatcg ctaacgagat aattgaatta atgattaaaa acggcgcacg
    18841 ccttgcgggg cgcggagagt ttactaatcg cgcatattta aacggcaagc tcgatttagt
    18901 gcaggccgaa gctgttaacg atatgattac cgcgacgaca aaagaagcaa agaaactttc
    18961 actattatcg ctagtcggtg aaacgagtaa aatcgtttat ccgataagag aaagaatcgc
    19021 ggacgtgatg agtttaatcg aagttaacat tgattatcct gagtatcgcg acattgaaga
    19081 agcaagctac gcacttgtta ttagtgaagt tgattatata aatgcggaga ttaagaaatt
    19141 gattgatcaa ggacgacaag gtcaaataat caaagaagga attaaagtcg ctattgttgg
    19201 tgctcctaat gttgggaagt cgtcattatt aaatgcatta cttaaagagg ataaagcaat
    19261 tgttactgat attgctggta caacgcgtga tgttgtggaa ggtgaaatta atttacacgg
    19321 acttcgcctt cttcttctcg ataccgcggg aattagagaa agcacagatg caattgaaag
    19381 tatcggtatt gaaaaaacaa agaaatcaat tgcagacgct gatttagtaa tatatgtaaa
    19441 agacgcagaa aatgtaagta aaaaagacga atttactggt ttaattaaag gtaagaaagt
    19501 aattaccgtt tataacaagg gagacttagt aagtaagaaa gatcctaatc gactttacat
    19561 ttcagcgctc actaatgata ttggtgcctt agaaaaagca attgctaaag ttgtaggagt
    19621 tgatgaagat agttatttac gaccaagttt aaataatgcg cgtcaactag cgttgttgcg
    19681 caaagcttat ggcgaacttg aaatcgctaa acaagaggcg cttgatgaag tcgctattga
    19741 ccttgttagc gtgtctttac aacaagcttt ctttacaatc aaagaaatat taggagaaaa
    19801 cgttgataat gatttaagta aagaaatctt ttcgcgtttc tgtgtaggta aataaagatg
    19861 ttttttgata cacactgtca tttaaacgat aaaagatacg aaaacgatat tgaagggtat
    19921 gttgagcgtg ctaaagcagt gggcgttaat tatcttttaa ttgtcgcatg ggatgtaaaa
    19981 tctagtaaaa aagcaattaa aatcgctgaa aggtttaata atgtatacgc tgccgcaggt
    20041 attcacccag ttgacgctgt taaaacaccg aaaagtgatt taaaagaact tgaaaaactt
    20101 cttacccatc ccaaagttat agcagtaggg gaaataggat tagattacca ctggataaaa
    20161 gacgaaaaag aaagagcaat tgaacacgaa ttctttatag cgcaaattga gcttgcaaat
    20221 aaatataaaa aaccagtgct tattcatatg cgcgacgcta caagtgatac atatgaagtg
    20281 ttaaagagta atatcccaag gtatcaagga ataatgcatt cttacagtgg ttcaaaggaa
    20341 atggcgttgc gttttataga tttggggatg tatataagtc tagcgggacc agtaactttt
    20401 ttaaatgcgc gtgagccaaa ggaagtggca aaggttgtaa gcgaagaaag acttctactt
    20461 gaaacagatg cgccttactt aaccccgcat ccatatcgcg gcaaattaaa tgaaagtgca
    20521 aatatcccac ttattgctaa gaccatagca acgattcgta atgtttctgt cgagaaagtt
    20581 gcagagttta caacaaagaa cgctaaaaca ttatttaaat tatgagtagc aaaacattgt
    20641 taaattgtat ctttgttgcg gaaggaaagc gcgacgaaga aagactaaaa aagttagaca
    20701 tcccttacat agtaaggaca gacgggctaa atgttccacg tgaaacaaca aagcatttac
    20761 aagaattagc gaaaattcat gaggttataa tcttaacaga tccagacgga ccagggcaaa
    20821 agattgcaga caaactttta caagaaatac ctaacgctaa agtaataaaa gtaataaaga
    20881 aaaaagcagt aaagaaagga aaagttggaa tagaacatat tccgctaccg cttctagcgg
    20941 aattaattaa accttataag caagaaacat ttactacttc ttccgatgtt acacttataa
    21001 aattactaga tttagggtta tctgggccaa attcacaatt taaaaaaata aagttagcga
    21061 ctaaatatag tttattagta gcgtcactta aaaatatgtt aacgcaaata caactattaa
    21121 atataagtta taaagaatta aaggaagcac taagtgaata aagaagaaat cataacatta
    21181 ttagcaaagg aaaacttgtc gccacaaaaa agaaagggac aaaacttttt agtggatgaa
    21241 aatgtagcgc gtaagattgt cagctttctt gaacctaatt acattgatgt aattgaagtt
    21301 ggacctggac taggtgcact aacaaaagag ctgcttttaa agaccgtaaa ttttactgca
    21361 attgaaattg ataaaggttt tgtgcgagta ttaagagcaa agtatccaca ccttaacatc
    21421 attgagaaag atttcttaga atatagtgtt ccacgtgaaa caaaggctgt tattagtaat
    21481 attccttatt acatcaccac aaaaataata gaaaaggtgt tattagaagc agaaaacctt
    21541 aaaaagtttg tatttatgac acaaattgat gttaaagaaa ggttgttagc aaaaagcgga
    21601 acaaaagcat atagtccgct gcgggtttta cttagtttgc ttggtgatgt tgaacaaaaa
    21661 ctagtggtaa ccgctgataa gttttatcca gtgccacatg ttgattcagc ggtattttgt
    21721 tttacacgat ataaaaccga tattcctgtg cgtgaatttt attcgttttt aaataacgct
    21781 tttctaaatc ggcgcaaaac actatacaac aatttgaaaa acatatacga caaagaagat
    21841 atattaaccg ctttaaagaa acatagaatt aacgaaaacg ttcgagcaga agcactatct
    21901 cctaacgatt tattaacatt atttgaaaca ttacgataat taaaagggtt aaaatgacaa
    21961 tcaacaaaaa actcaaacat gagggaaaat tggaaatttc agcaatttat gatatattga
    22021 atatgtaaat atcaaatcta aggaatataa catgaaaaaa tactgcaaat tttcaactac
    22081 attgttgaat attttagtaa cggaagttat ttattatcga acggtgtcta tgttataagt
    22141 gacgctgatt tagatgcatt taatagtggt acacttgata ttccaaaaaa taatgaagaa
    22201 atatacaatt actgttgtta ataaaaaggg aggaaacgac aatgaaaaat aaaaagaaat
    22261 tgttttggtt aatattacca tttactttaa ttatgttcgc tcttttgttt gcgctttatc
    22321 ctacaccatg gggagccaaa gaaccataca aggtttttca ttcccctgaa aaacatttaa
    22381 aggaagttga aaaaagagtg aatcaaaaaa tagaagagcg agaaatggat tatacaaatt
    22441 tacgagcact tcctctttat gatgaatata ataaatttag ctttgtactt gtagaatttg
    22501 aaccccaaga ttttatgatt ttaaaggttt accgcactag tata
//
LOCUS       DAOPCD010000003        22034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_2508_length_22034_cov_5.044588, whole genome shotgun sequence.
ACCESSION   DAOPCD010000003 DAOPCD010000000
VERSION     DAOPCD010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 22034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22034
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2508_length_22034_cov_5.044588"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /locus_tag="PLQ55_00230"
     CDS             complement(<1..1548)
                     /locus_tag="PLQ55_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK28402.1"
                     /translation="MARHGRGRKMEKARNSKLAWRGLLREFKPYRWRIIIIAVLLTIA
                     NVANALIPLLLRNILGAFSPENVRYGRYFLDVTNLALGLDWTKVFIDFGLMIGVIVAA
                     ALLGTIAQLIAIKISGAYAYNLRNKIRAKIDRLPLSYFDKHPHGELLSIGTNDVDAIA
                     QTLNNTLITVVSSITLFVAILIGMLLVNWRLALVALGMLPFVVIITLVITKNSQNQFI
                     TFQKKTGELEGVVEENFSGLQVIQLFNQQDKQLETFTNLNDSMAKSNFWSQWLSSFIF
                     PSIRFINNLGFVGVLLVAGVINDVISLAPFIIFLNNFMQPFLNLGQISANIQTTLAGA
                     ERVFNLLNAAEQEKDPEDALTTNDHVVGEFCFKDVDFSYNPDVELIKNMNLHVKPGDT
                     IAIVGPTGAGKTTLVNLIMRFYEINGGSITLDGTDIRNYSRSALRRSVGMVLQDTWLF
                     KGSIKNNIRYGNRDATDEEVIEAAKAARAHHFITTFPGEYEFILNEDGTNISQGQRQL
                     ITIARAII"
     gene            complement(1548..3371)
                     /locus_tag="PLQ55_00235"
     CDS             complement(1548..3371)
                     /locus_tag="PLQ55_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK28403.1"
                     /translation="MFRLYKLVKRYWWQLAIIVVLVATQTLLQLQLPDYITTTQNIVN
                     NIGETGITRAEINEMLKQSGIMLLISLGIFALAAGQGSLNSKFVATLGKEIRAEVFKK
                     VENFSLSDVNEFGTASLLTRLINDSRAVQEFAFMVNRVFIMSPIFLIIGSVKLLTLDP
                     MYFYVVIVIIPLIIIVLIITFAVASPLFTQIQERVDYVTLLLREGLTGVRVIRAFNQE
                     ERENRFFDEGNRKMTDIIIKVSNVMQIISPIINILFNLVFFGIYLIGFVLFDKMLLGV
                     IPFNMNAFSQTITAMMYAQHIMMSFMMLSFTFIMFPRVKVSAKRINAVLNVKPNIVDP
                     EAPLDKELIDTGIVKFENVTFTFSDLEHPTLKNINFTAEPGTTTAIVGSTGSGKSSII
                     NLLPRFFDASVGKVTFDGYDVRDFKLNDLRDKIGFVPQTAVLFKGTIRDNLRFGNENA
                     TDEEMWEALRVAQAAGFVSKLELGLDAPVAQGGKNFSGGQRQRLAIARALVRKPKIYV
                     FDDSFSALDFKTDIRLRSALKDYAKDATIFVVAQRVNSILDAHQILVLDNGEVAGIGK
                     HADLLNSCKVYQEIVTSQLDPEEIKKSRDMFKDFTTEGGKA"
     gene            complement(3372..3980)
                     /locus_tag="PLQ55_00240"
     CDS             complement(3372..3980)
                     /locus_tag="PLQ55_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPK28404.1"
                     /translation="MARRTTSRTKSTTKTRRDDILEAGIRLFARVPFSELTIAAVARE
                     ANCGHSLVYHYFTNINRLYDECVNHVVARYKPFIEHFKSLTIPAELALAGSIALIVEM
                     LKREKLAPYCLSLLLYTYKQAPRNKAILTVREDWSKLFLNFISEGQKNERIISTLSAN
                     EILNVLKSILRGLISDQIVSGPSPANILTASEIYLPIMQGIN"
     gene            complement(4017..4709)
                     /locus_tag="PLQ55_00245"
     CDS             complement(4017..4709)
                     /locus_tag="PLQ55_00245"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPK28405.1"
                     /translation="MRLDRFLAKTLGETRTTVKKYIRTKKVTVNGEVIISDGFQVDTI
                     NDVVAFDGEVLQYEEFQYYLINKPEGYVCANEDNVNPTIISFAPEFMMYKLHTVGRLD
                     KDTTGALLLTNNGKLTHQLISPKKNVEKVYLATTDLPIKGELVAHFARGIKIDEDFTT
                     LPATLEIVESNVGRVTVVEGKFHQIKRMFQAFGLNVVKLHREQFAGLRVDDLEIGEYR
                     KLEHDEINSISI"
     gene            complement(4706..5380)
                     /locus_tag="PLQ55_00250"
     CDS             complement(4706..5380)
                     /locus_tag="PLQ55_00250"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPK28406.1"
                     /translation="MEWEITFLRWVVENTQTPFITAIFKFLTLSGELAVLYFIIAFIM
                     IGFKKTRKAGLIIVFGLILVSGFNLWIFKPIVNRPRPFDVPEFSADALWLNNYMYNVF
                     APQSKLNDFLVPGSKAFMSGHTLSSFIFGFTILFHHRKWSVPALILSVIMSYTRIYFG
                     FHYPTDVFAGIITALATVLLFTWLANKHETWFINLWRKFISLFKKKSKVDDTTAIETE
                     HQEEAQ"
     gene            complement(5405..6334)
                     /locus_tag="PLQ55_00255"
     CDS             complement(5405..6334)
                     /locus_tag="PLQ55_00255"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HPK28407.1"
                     /translation="MNRFLKRFLQGLLLGFALITAIGGGTMAVLIGVYDELINAVSDF
                     RKDPKKSLSLLGPMALGGIVSMGALFYPIKLALEHFPFLSISFFAGLTLGGLRIFTGT
                     IKGNVTWKNLLLTLAGFLFVFGLGTFTWFTTLHADLVNINFLQVILLFFVAFLSMGGH
                     VSAGISGTFILIAFGYYEELIDFLIRLAKFDFVNIGFDLAGLLSFGIGSILGLIVIAK
                     TYKYFFEKDRVKTYFTIFGFIIGSIVIAFFNGKVRPEYAKVTGFDPLMFTLCVVAVIV
                     GAALSYFLLGLANKKEKQQTVEEVAINSNNKED"
     gene            6381..6962
                     /gene="pth"
                     /locus_tag="PLQ55_00260"
     CDS             6381..6962
                     /gene="pth"
                     /locus_tag="PLQ55_00260"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006506439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPK28408.1"
                     /translation="MKLFVGLGNPGKKYENTRHNAGFLALDKFAEIAKVDIDKSDFKG
                     LFTSFTYNAEKIYLLKPQTYMNLSGQSVVSLMNFFKIEVDELYVIYDDMDISPGQIRL
                     KEDGSSGGHKGMQNIIELLGTTNIKRIRIGIGKPEHDGVDHVLQKLTDEDKKIFMEGV
                     TKASVAVRDLLNEDFNTVMNKNNRKEPKPPPSD"
     gene            6955..10428
                     /gene="mfd"
                     /locus_tag="PLQ55_00265"
     CDS             6955..10428
                     /gene="mfd"
                     /locus_tag="PLQ55_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPK28409.1"
                     /translation="MIKLFETLFPKNTPPRFEKKRVFVTSEIIGTTLLVAEAFKKKRE
                     NYVVLTNNLYNAQKIISTLQTFVSQDDILFFPNDDLLFTEVLTATKELEAQRIYTLFS
                     SLEKDKKYIYVANQSALLKSTLSPTSFAKQFFELKVGNTVKMSALTTKLVELGYKNVT
                     NVEQTMQFAKRGDIIDIYTVNEDFPTRIEFFDDEIESIRDFNVSTQVSIKTRDKVTML
                     PASENLLFPNEQKEFLKCLQEFFNVQKKDMTSAEEEMFATNLNASISSYLEGDNNALN
                     KYLLIYSLFQNSLLSYIDNPKVVFANEPQLLATYEIISNHAEHYVRDQARRFNLLPKT
                     QLYQPYNTLDHYSKYVTREFSEDGNEGELRISPVADLRSRDDFNLVVQKYYDDNYLIY
                     FSVNNNTQLEQVKNLLKELQLDFDLDDKSSDKRILITYDYFPHGFVINSCKTAVFTSK
                     ELFNITRNEMRFNARFKAGTVIRSHEQLTPGDYVVHEYHGIGQFMEIESLKVFGVVSD
                     YIKIKYFGNEELSIPMEQIHLIRKYSGREGVAPRLHKIGSPVWQRTKVEISKKIEDIA
                     EELIAIQAARYQRPGFAFPPDDEFQKEFELGFPYELTYDQEIALREIKNDMESPLIMD
                     RLLSGDVGFGKTEIALRAAFKAISAGKQVMFLCPTTLLARQHYEVSLERFANFGVKIA
                     MISRLVTQKEKDEIVKDFQAGNIHLLIGTHALFNIDVESVDLGLLIVDEEQRFGVEQK
                     EIIKKMKTRVDVLTLSATPIPRTLQMSLVGIRGLSQINTAPQERMPIQTYVIKRDDYV
                     IKELVERELGRGGQVFYLYNRIATIYHVANKLSRLVPQAKIGVVHGKMDRADIEDVMT
                     SFYNNEINVLVATSIIENGIDVPNANVIIVENADTFGLSQLYQIKGRVGRGNRIAYAY
                     LLYQGNKIMNDDAKKRLQAIQEFTDLGSGYKIAQRDLMIRGAGDILGAEQSGFIDSIG
                     IDMYLKMLEDAINEKKYKLTSDPIEKTINISGLEAYIPDDFASKSEKFDIYLEIKKSK
                     KMKELKDFEEKVIDMYGKLPRSLEILFLKRQVELVVNSRANFIEDIKDEDVFIDIIPG
                     RAFLDIPGSVVMLNNEIGTTYQNIRFITQDDKIKLRIIKRGDWFHAYASIVKAIVMIV
                     RETMKNKDETR"
     gene            10466..10675
                     /locus_tag="PLQ55_00270"
     CDS             10466..10675
                     /locus_tag="PLQ55_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28410.1"
                     /translation="MAKDISTAGKVIVDKRTVKPSYTVKVDDVLELYLGAHIVVVKVL
                     TVDEKEIKSNPEKGYELLSRTKNAN"
     gene            10665..11891
                     /gene="tilS"
                     /locus_tag="PLQ55_00275"
     CDS             10665..11891
                     /gene="tilS"
                     /locus_tag="PLQ55_00275"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPK28411.1"
                     /translation="MQINLDHNKKYLLACSYGPDSMALFSLLLKGNYNFSVAHINYGL
                     RGEESEKETNDLALYCKEHNIAFFVKWVNGKKIKGNFQNEARKVRYEFFEEIIKEHDF
                     DALLTAHHADDVIETYLMQQNSKRETFYYGIREEIVLNNFLVIRPLLNYYKNDLVFYC
                     VEHKVPFAVDSSNLELTYTRNKIRHNLVKKMSVEQKDKLLLDIKGRNKDLKAMTNKLE
                     LLLENSTISITEFIKLNNREQQHLLYMLFHNKGIADKFSKGRNNQIKDVVLSDKNSAM
                     IKVIDSYYLVKYEDKFSLININNYQPYKYIVETPETIKTPQFVAFFGKNNVFPNIPAS
                     EYPLTIATQGKNQKYKIKGYEKTINRLFIDMKMPRHYRLIWPVVTNKNGEVIYVPRYR
                     ANYIPKATDLFKIYIK"
     gene            11994..13979
                     /gene="ftsH"
                     /locus_tag="PLQ55_00280"
     CDS             11994..13979
                     /gene="ftsH"
                     /locus_tag="PLQ55_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPK28412.1"
                     /translation="MSNPNPRPNPRSVPPRRSLRTLLPYLLLFAVIGVVIWLAFFTGN
                     AKTQALLQTELAAYLQTNAGVIDTITIVTTETTTKITGILKDGSKFTALVDRTFYENI
                     LQPTLFAADGTALVDFKMVIVNAYQRTFWNTIGPSLLYLVLFVVAMIFLYSRMAGAVS
                     SSNTKAMEFNRSRARLETKVKKTFDDVAGADAEKQEMMEIVDYLRNPKKYTKFGAKLP
                     KGVLLVGPPGTGKTLLAKAVAGEANVPFYSISGSDFVEMFVGVGAGRVRDMFQQAKKT
                     APCLIFIDELDAVGRQRGAGLGGGNDEREQTLNQLLIEMDGFSDNSGIIVLAATNRDD
                     VLDPALLRAGRFDRKITVSLPDRAGRTAIFGVHARGKIIDESIDFDAISRRTVGFSGA
                     DIESVLNDAAILAIRDNADKIMLQHIDEAIDRRIAGPARTSRQLNEHERKVIAYHEAG
                     HAVVGLNHPFSDKVQKITIIPRGQTGGHVLMTPEEDRFLMTKNQLIARIVGYLGGRTS
                     EEVFFDDVSTGAQNDIEVATRIARLMVTEFGMSDLGPIQYERDTGSVFLGRDYTSSQK
                     NFSQQVALEIDKEVRKIIEQAHDKAREIIEKHKDDVILIAETLLDRETITAEQIDRLL
                     KDRKLDEPKQAVIGEVVLTEKPKVEPTPTPVPEDDKK"
     gene            14018..14998
                     /gene="dusB"
                     /locus_tag="PLQ55_00285"
     CDS             14018..14998
                     /gene="dusB"
                     /locus_tag="PLQ55_00285"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPK28413.1"
                     /translation="MWKIGNIEIKGKTILAPMAGITTFAYREFMRSFGVALCYTEMIS
                     DSGLIFNNEKTYEYLPRPEEKRPTSVQLFGGETTKLLVAIEKIEKHTDNYDFLDLNLG
                     CPVPKVTRNNGGSAWLKHPNELIAMVEKVVKRSTKPVTVKIRIGWDEKHINFKELLPK
                     LEEAGVSLIAIHLRTTKQLYGGKARYDLAQNIQNLVSIPLVVSGDIYTLDDAIKALEI
                     TGAQAVMLARGQLGNPFLATQIETYFKTGERLKDPSLYQQLDYLWTLANMMVEEKGEH
                     MGILVMRGIAHHFIKGFPFTKTYKVKLTKMSTLNDLELIIKEIKNQALLI"
     gene            15039..16523
                     /gene="lysS"
                     /locus_tag="PLQ55_00290"
     CDS             15039..16523
                     /gene="lysS"
                     /locus_tag="PLQ55_00290"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPK28414.1"
                     /translation="MPREFSDQELVRRAKAEELSAQGIDPFGKKYERTDTSLSIKEKA
                     GEFTKEELEEKAIKVKTAGRIMLMRIMGKASFFTIQDQEGRIQAFISKQGVGEEVYDL
                     FKSADIGDIVGVEGTIMRTKTGEVTVKVEVYTHIVKALRPLPEKFHGLTDVEERYRRR
                     YVDLIMNESSKHVALTRPKIIRAIQKFLDDRGFVEVETSMLNSVLGGASAKPFITHHN
                     TLNRDFYLRIATELPLKRLLVGGIEKVYEIGRLFRNEGMSTRHNPEFTTVEAYQAYAD
                     LDDMYELCESLVRELAIKVTGDTKLPWGDEIIDVGAPFRKVSMVDLVKEKTGVDFRKI
                     KTFEEAKAIADKHEIHVEKHWTGSGHILNAFFEEFCEADLIQPTFVYGHPLEISPLAK
                     KAEDPRFTERFELFIKANELANAFTELNDPRDQYERFKAQLKARELGDEEASEMDEDF
                     VEALEYGMPPAGGIGIGIDRLVMFLLNQTSIREVILFPTMRDNK"
     gene            16672..16959
                     /gene="rpsF"
                     /locus_tag="PLQ55_00295"
     CDS             16672..16959
                     /gene="rpsF"
                     /locus_tag="PLQ55_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPK28415.1"
                     /translation="MKKYEIMYILLADLSEEERKAEMESLHQMITNLGGKIVDVDTEN
                     WGLRDFAYPINDLLKGYYVVINVESDAAAIAEFERLSKLNKNVIRHLVIAK"
     gene            16978..17391
                     /gene="ssb"
                     /locus_tag="PLQ55_00300"
     CDS             16978..17391
                     /gene="ssb"
                     /locus_tag="PLQ55_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014645360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPK28416.1"
                     /translation="MINRVVLVGRLTHDPELRKTSSGASVTNFSIAVNNRFARDQENS
                     TSFFRVSCWNQTADFVAQYARKGNLVGVDGRIEQRSYVNNAGQKVNTVDIIADSVQLL
                     ESKSESSEGTGYQSDAEPATDLGEGIDISDDDLPF"
     gene            17416..17643
                     /gene="rpsR"
                     /locus_tag="PLQ55_00305"
     CDS             17416..17643
                     /gene="rpsR"
                     /locus_tag="PLQ55_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPK28417.1"
                     /translation="MSYRKSRPRRKVCYFKQNKITEIDYKNVELLKRFVTPNGKITPR
                     RVTGTSAKYQRQLAVAIKNARFMGLLPYYVS"
     gene            17698..18651
                     /locus_tag="PLQ55_00310"
     CDS             17698..18651
                     /locus_tag="PLQ55_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28418.1"
                     /translation="MKKTKFYYFELGLVILITLGLYVFSTNFLLLHYIGFLGPVPLAV
                     FIIRHKIKDGIFPAIFMILMGTLIGHFLPVGQGSFMRGFLFMIYAMTVGFLHGTISKT
                     KINPLIEILIIIGVEIFYGFLLVIVFYVQKDPHFAYDIQFGQYLELFIKLFKIDETSN
                     YAQNVGVIIKNGVIAYTIVFSFISVLLTHILIHLTRKFIYREKMEHGPLHGLEFTISR
                     VAAITYLVGCVVVLVLSFLLTKELNLLGISSINAIFTVFFSVTIIFVFQGILAVNLRL
                     EAKGCNNLSTLVFISGILLSPIFAIIGAINPLLPRKEKAVV"
     gene            18733..19329
                     /locus_tag="PLQ55_00315"
     CDS             18733..19329
                     /locus_tag="PLQ55_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28419.1"
                     /translation="MARKKKNDVALYDDSSYEEDDLKYAADEEVYAESLEDDEGKAKK
                     KRKKKKKEPKDPKERKDGSETRGGKTLTDRYHYRGRYYLLPERELRKQLRFYQKVFNY
                     RFLGIIAGVIALIFSILSLTFNAYFAVFTLLIVITGLIYGLLGMLKLRGLNWLFVLIG
                     IGLNIMAIVLAMKPLTFVFKHLGEIMALINEYMSSAGS"
     gene            19451..21538
                     /locus_tag="PLQ55_00320"
     CDS             19451..21538
                     /locus_tag="PLQ55_00320"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPK28420.1"
                     /translation="MIKAITKLRKRTIILVIVEGLLLGLISLFYYLNLFNFQEIFVPP
                     VIFSVAIGVVFFNLFVFIFITARINKLRKKNTFELVNLLGGDVKEAYNFGMLGLVVVD
                     NDFNVTWASELFKERRLLIIDKNILAWKPELIRLVELSEVLADNVEDTIKVEIESRNY
                     EVKYLSKAGIFLFKDITEFEYLFDSNQATAPVLGVVVIDNFVDIVVKGEHSNPIFGTV
                     NSLILEYFSNYGVLLKQYREDAYLLILNKENFDKLYADNFSILQKIKDKTSNQDYQIS
                     LSMAFAYNFPDVLKLNELVLNAIDTAMARGGDQVVIARYGEESEFIGGATSQAEKRNK
                     TAIRLYADTIFRHIREAKNVLIMTHTDTDFDALGASLGLKAMADYVKKTQKGTKVQFQ
                     PARIVYEHNLTELKTRMAVNRMFTKQEMNDMFISPEEIDDQKGTASLVNSGTLLIIAD
                     VSCPQLTLHPKLLERVDKVIVIDHHRRTDEFIDQRLYDYIETSASSTCEIVTELLHYG
                     NTPNFVLPPKYATFMLAGIYLDTNYFRTKTTGSRTFLASMVLEDWGADVMTADDFLKD
                     NVEEYALITKIMNSMTTLSVGIVFLRADETDIVTPETLSKVANQSMRIKGIQASFVIG
                     YTAPKVVKVSGRSNGEINVQFLLEKVGGGGHQASAAAVFTDKTLDDVQEILEDVINQN
                     LSQAKVVGGKKGG"
     gene            21540..22001
                     /gene="rplI"
                     /locus_tag="PLQ55_00325"
     CDS             21540..22001
                     /gene="rplI"
                     /locus_tag="PLQ55_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPK28421.1"
                     /translation="MKVILLKDVKKVGKKDEIVDVSDGYATNFLIPRKLAVPYNERTI
                     SQLKANQARALAEEKAHKEEALVNKKKLEEKEISFILAIGEKGKAFGAITAKKVEEKI
                     KEEFKINVDRRKFVNFQPLNQLGPTIVEIELFKDVIAKVKVLVKDKEINYS"
BASE COUNT         7737 a         3656 c         4011 g         6630 t
ORIGIN      
        1 aataattgca cgagcaatcg taattaactg gcgttgccct tgcgaaatat tagtgccatc
       61 ttcattaagt ataaactcgt attcacccgg aaaagtagtt ataaagtggt gagcgcgcgc
      121 tgctttagca gcttcgataa cttcttcgtc agtagcgtca cgattaccat aacgaatatt
      181 atttttaatc gaacctttga agagccaagt atcttgtaaa accatcccaa cactacggcg
      241 taatgcactt cgtgaataat tacgaatatc agtaccatct aatgtaattg aaccaccatt
      301 tatttcatag aatcgcataa ttaaattaac tagtgttgtt ttgcccgcgc cagttggacc
      361 aacaatagcg attgtatccc ctggtttaac atgtaaattc atatttttaa taagttcaac
      421 atcagggtta taactaaaat caacatcttt gaagcagaat tcccccacaa catgatcgtt
      481 tgtagttaat gcatcctctg gatctttttc ttgttcagcg gcattaagta aattaaacac
      541 tctttctgca ccagcaagtg ttgtttgaat atttgcagat atttgcccta agttaaggaa
      601 tggttgcata aagttattta agaaaataat aaatggcgcg agactaataa catcatttat
      661 aacacccgcg acaagtaaaa caccaacgaa gccaagatta ttaataaagc gaattgatgg
      721 gaaaataaat gaactcagcc attgtgacca gaaattagat ttagccatag aatcattaag
      781 atttgtaaaa gtttctagtt gtttatcttg ttgattaaat agttgaatga cttggagtcc
      841 ggaaaagttt tcttccacta ccccttctaa ttcacctgtt ttcttttgga aagtaataaa
      901 ctgattttgt gaatttttcg taataacaag cgtaataatt actacaaacg gaagcatgcc
      961 aagtgctact aaggcaagcc gccaatttac aagtaacatg ccaattaaaa ttgcaacaaa
     1021 aagagtgatt gatgaaacaa cagtgataag cgtgttattt aatgtttggg caatagcatc
     1081 aacatcattt gtgccaattg aaagtaattc accatgcgga tgtttatcaa aataagaaag
     1141 tggtaaacga tcaattttcg cgcgtatttt attacgaaga ttataagcgt acgctccaga
     1201 aattttgata gcaatcagtt gtgcgatagt accaagtaat gcagcggcaa caataacacc
     1261 aatcattaat ccaaaatcaa tgaagacctt tgtccaatct aaccctaacg ctagattagt
     1321 aacatctaaa aaataacgac cataacgaac attttcaggc gagaaagcac ccagaatatt
     1381 acgcaataat agcgggataa gcgcattagc tacatttgcg atagtaagaa gcacagcaat
     1441 aattattatc cgccaacgat aaggtttaaa ttcgcgcaaa agaccacgcc aagctaattt
     1501 tgaattgcgc gctttttcca ttttgcgtcc gcgtccgtgg cgtgccatta tgctttaccc
     1561 ccttcagttg taaagtcttt aaacatatca cgcgactttt taatttcttc aggatctagt
     1621 tgtgatgtaa cgatttcttg atatacctta cacgaattaa gtaagtcagc atgtttgcca
     1681 ataccagcaa cctcgccatt atcaaggact aagatttgat gggcatcaag aatagaatta
     1741 acacgctgtg ctacaacaaa aatagttgcg tcttttgcgt aatcttttaa tgcgcttctt
     1801 agtctaatat cagtcttaaa gtctagtgca ctaaaagaat catcaaacac ataaatttta
     1861 ggttttctaa caagtgcacg cgctatcgct aggcgttgtc tttgaccacc actgaaattt
     1921 ttgccacctt gtgcaactgg tgcatcaagt ccaagttcga gtttagaaac aaaacccgca
     1981 gcttgcgcca cgcgtaaagc ttcccacatt tcttcatcag tagcgttttc attaccgaag
     2041 cgtaaattat cacgaattgt acctttaaat aacactgcag tttgtggaac gaagcctatt
     2101 ttgtcgcgta aatcatttaa tttaaagtca cgcacatcat agccgtcaaa agttacttta
     2161 ccaacactag catcaaaaaa tcgtggtaat aaattaatga ttgaactctt gccagaacca
     2221 gtagaaccaa caattgctgt tgtagttcct ggctctgctg taaaattaat attctttaat
     2281 gttgggtgtt caagatcgct gaaagtaaaa gttacatttt caaacttaac aataccagta
     2341 tcaattaatt ctttatcaag cggtgcctcc ggatcaacaa tatttggttt aacatttagc
     2401 acagcgttaa ttcgctttgc gctaacttta acgcgcggga acataataaa ggtaaagcta
     2461 agcatcatga aactcatcat aatatgttga gcgtacatca tcgctgttat tgtttgacta
     2521 aaagcattca tattaaaagg aataacacca aggagcattt tatcgaacaa gacaaaacca
     2581 ataagatata ttccaaaaaa cactaagtta aataagatat taataatcgg acttataatt
     2641 tgcataacat tactaacttt aataataatg tcagtcattt tacgattgcc ttcgtcaaag
     2701 aagcggtttt cacgttcttc ttgattaaag gcacgaatga cacgaacacc agttagtcct
     2761 tcgcgtaata atagcgtcac ataatccact cgttcttgaa tttgggtgaa aagcgggctt
     2821 gctacggcaa aagtaataat caatacaatt ataatcaaag gaataataac gataacgaca
     2881 tagaaataca ttggatctaa tgttagtaat ttaacagaac caataattaa aaagattggt
     2941 gacataataa aaacacgatt taccataaaa gcaaattctt gcacagcgcg tgaatcgtta
     3001 attagcctag ttaaaagact agcggttcca aattcattaa catcacttaa tgaaaagttt
     3061 tcaacttttt tgaacacttc cgcgcgaatt tctttaccaa gggtagcaac aaatttagag
     3121 tttaatgagc cttggcctgc tgctaaagca aaaataccaa gggaaattaa aagcataata
     3181 ccgctttgtt ttaacatttc atttatttca gcgcgagtta tcccagtttc accaatatta
     3241 ttaacaatgt tttgagttgt tgtaatataa tcaggtaatt gaagttgaag caatgtctga
     3301 gtagcgacga ggacaacaat aatcgctaat tgccaccaat aacgtttaac taatttataa
     3361 agtctaaaca tttagtttat cccctgcata attggtaagt aaatttcaga cgctgttaag
     3421 atattagcgg gcgacggacc agaaacaatt tggtcactaa ttaaaccgcg taatattgat
     3481 tttaaaacat ttaatatctc attagcgctt aatgtactga ttatacgctc gtttttttgg
     3541 ccttcactaa tgaaatttaa aaaaagtttt gaccaatctt cccgcactgt taatattgct
     3601 ttattccgtg gtgcttgttt gtaagtatat agcaacagac ttaaacaata gggagctagt
     3661 ttctcccttt ttaacatttc tacaatcaaa gcaattgaac ccgcgagcgc tagttcagcg
     3721 ggaattgtta atgatttaaa gtgttcaata aaaggcttat aacgagcaac aacatgattg
     3781 acacattcat catataagcg attaatattt gtgaaataat gataaacaag actatgacca
     3841 caattagctt cgcgcgcaac agcggcaatt gttaattcag aaaaagggac acgagcaaaa
     3901 agtcgtattc ctgcttcgag aatatcgtcg cgtcgagttt ttgtggtact tttagttcta
     3961 cttgttgttc gtcttgccat agttatacca ttgcaaaaat tataacaaag atgaacctaa
     4021 atagatattg aattaatttc gtcatgttct aattttcgat attcaccaat ttctaaatca
     4081 tcaacacgca gtcctgcaaa ttgttcgcga tgaagtttta caacatttaa cccaaacgct
     4141 tgaaacatcc gttttatttg atgaaattta ccttccacaa cagtaactct acctacatta
     4201 gattccacta tttctaaagt tgcaggaagt gtcgtaaaat cttcatcaat tttaattcca
     4261 cgcgcaaaat gcgcgactaa ttcaccttta ataggtaaat cggtggtagc aagataaacc
     4321 ttttcaacat tcttttttgg acttataagt tggtgtgtaa gtttaccgtt gtttgttaat
     4381 agtaaggcgc ctgtcgtatc cttatctaaa cgaccaacag tatgtaattt atacatcata
     4441 aattctggcg caaaagaaat aattgtaggg ttaacattat cttcgtttgc acacacatat
     4501 ccttctggct tattaattaa ataatattga aattcttcat attgtaaaac ttcaccatca
     4561 aaagcgacga catcattaat agtgtccact tgaaaaccgt ctgaaataat aacttcacca
     4621 ttgacagtca cttttttagt gcgaatgtat tttttaacag tggttctagt ttcaccgaga
     4681 gtttttgcta gaaaacgatc taatctcatt gtgcttcttc ttgatgctca gtttcaattg
     4741 cggtagtatc gtctacttta gactttttct tgaagagact aataaattta cgccaaagat
     4801 tgataaacca agtttcgtgt ttatttgcta accaagtgaa aagaaggaca gtggcaagag
     4861 ctgttatgat acccgcaaag acatcagtag gataatggaa tccaaaataa atccgggtgt
     4921 atgacattat tacacttaaa attaaagcgg gcactgacca tttccgatga tggaataaaa
     4981 tagtaaatcc aaaaataaaa gatgaaagcg tatgtccaga cataaatgct tttgaaccag
     5041 gcactaaaaa gtcattaagc ttactttgtg gcgcaaagac gttatacata tagttgttta
     5101 accataaagc atccgcagaa aattcaggaa catcaaaagg tcgcggccga ttaacaatcg
     5161 gtttaaaaat ccaaagatta aaaccagata caagaattaa accaaaaaca atgattaatc
     5221 ctgcttttct cgtcttttta aatccaatca taataaaagc aattataaaa tagagaactg
     5281 caagttcgcc agagagtgtt aaaaacttaa aaatagcggt aataaacggt gtttgtgtat
     5341 tttctacaac ccagcgtaaa aaagtaattt cccattccat agttaagtac tcctttttta
     5401 tattctaatc ttctttatta ttactattta tagcaacttc ttcaactgtt tgttgttttt
     5461 cttttttatt tgctaaacct aaaaggaagt aagaaagcgc cgcgcctaca ataacagcaa
     5521 caacacaaag agtaaacatt aaggggtcaa aaccagtaac tttagcgtac tctggccgta
     5581 ccttaccgtt aaagaaagca ataacaattg agccaataat aaaaccaaag atcgtgaaat
     5641 aagttttaac gcggtctttt tcaaagaaat atttatatgt cttagcaata acaattaacc
     5701 ctaaaattga accgatacca aacgataaga gtcccgctaa gtcaaaaccg atattcacaa
     5761 aatcaaattt agcaagtcta attaagaaat caattaattc ttcataatat ccaaaagcaa
     5821 tgagaataaa tgtacctgat atacctgcag aaacatgacc gcccattgaa agaaaggcca
     5881 caaagaaaag caagatgact tgtaagaaat taatatttac taaatcagcg tgtaaagtcg
     5941 taaaccaagt aaaggttcct aagccaaata caaaaaggaa acccgcaagt gttaaaagta
     6001 agtttttcca agtgacattg cctttaatag taccagtaaa tattcgtaaa ccgccgagtg
     6061 ttaaaccagc aaaaaatgaa attgaaagaa aaggaaagtg ttcaagcgct agtttgattg
     6121 gataaaaaag cgcgcccata cttacaatac cgccaagcgc cattggtccg agtaaactaa
     6181 gcgacttttt tggatctttc ctaaaatcag aaacagcatt aattaactca tcataaaccc
     6241 caattaaaac agccatagtc ccgccgccaa tcgcggtaat taaggcaaat cctaagagca
     6301 aaccttgtaa aaaacgtttt aagaatctat tcatatcata aataatagca caataaatgt
     6361 tataatttat cagtaaaaat atgaaactat ttgtcggttt aggtaatcca ggaaagaaat
     6421 atgaaaacac gcgccacaac gctgggtttt tagcgcttga taaatttgcc gaaatcgcta
     6481 aggttgatat tgataaaagt gatttcaaag ggcttttcac gagttttact tacaacgctg
     6541 aaaaaatata tttattaaaa ccacaaacat atatgaatct ttctggacaa tcagtagtgt
     6601 cactaatgaa cttctttaaa attgaagttg atgaacttta tgtaatttat gatgatatgg
     6661 atatttctcc aggacaaatc cgtttaaagg aagacgggtc atctggcgga cacaaaggaa
     6721 tgcaaaatat tattgaactc ttaggaacca caaatattaa acggattcgt attggcattg
     6781 gtaaaccaga acacgacgga gtggatcatg ttttacaaaa acttactgat gaagataaaa
     6841 agatttttat ggaaggcgtc actaaagcaa gcgttgccgt tcgtgatttg ttaaatgaag
     6901 atttcaatac tgtaatgaat aaaaacaacc gaaaggagcc aaaaccccca ccgagtgatt
     6961 aaactttttg aaacattgtt tcctaaaaat acaccccctc gctttgaaaa aaagcgagtt
     7021 tttgtcacaa gcgaaatcat agggacaacg ctattagtcg ccgaggcttt taagaaaaag
     7081 cgagaaaact atgttgtttt aacgaacaat ttatataatg cacaaaagat aatttcaacc
     7141 ttacaaacat ttgttagtca agacgatatt ttatttttcc caaatgacga tttattattt
     7201 acggaagtat tgaccgcaac taaagaacta gaagcacaaa gaatttacac attattttca
     7261 tcacttgaaa aagataaaaa atatatttat gttgctaacc aatcagcgct gcttaaatca
     7321 acgctttctc caacatcttt tgccaaacaa tttttcgaac taaaagtagg aaatactgtt
     7381 aaaatgtcag cgttaaccac caaattggtg gaattaggat ataaaaatgt cactaatgtc
     7441 gaacagacga tgcagtttgc aaaacgcggt gatattatcg atatatatac agtaaacgaa
     7501 gactttccga cgcgtattga attcttcgat gatgagattg aatcaatccg tgactttaat
     7561 gtaagcacac aagtatcaat taaaactcgt gataaagtaa ctatgcttcc cgcaagtgaa
     7621 aacttattat tccccaatga acaaaaagaa tttcttaagt gcttgcaaga atttttcaat
     7681 gtacagaaaa aagatatgac tagcgcggaa gaagaaatgt ttgctactaa cttgaatgca
     7741 agtattagta gttatttaga aggtgataat aacgctttaa ataaatatct attaatttat
     7801 tcattgtttc aaaatagttt gctctcttat attgataatc caaaagttgt attcgctaat
     7861 gaaccacaat tacttgctac atatgagatt atttctaatc acgcagagca ttatgttaga
     7921 gaccaagcaa gacgatttaa cttgttacca aagacacaac tttatcaacc ttataacact
     7981 ttagaccact attcaaaata tgtaacacga gaatttagcg aggacggcaa tgaaggtgaa
     8041 ctccgtataa gtccggtagc ggatcttcga agtcgtgatg attttaattt agtagtgcaa
     8101 aaatattatg atgataatta tcttatttat ttctcagtta acaataacac acaactagag
     8161 caagttaaaa atttattaaa agaattacaa ttagattttg atttagatga taaaagtagc
     8221 gataaaagaa tattaattac ctatgactat tttccgcatg gatttgtcat taattcctgc
     8281 aaaacagctg tatttactag taaagagcta tttaatatta cgcgtaatga gatgcggttt
     8341 aatgccaggt ttaaagccgg aactgtgatt cgtagtcatg aacaacttac cccgggtgat
     8401 tatgtagttc atgaatatca cggtattggt caatttatgg aaattgagtc cttaaaagta
     8461 tttggtgttg ttagtgacta tattaaaatt aaatattttg gcaatgaaga attatcaatt
     8521 ccaatggaac aaattcattt aattcgcaaa tatagtggcc gagaaggcgt tgcaccgcga
     8581 ctgcataaaa ttggttcacc agtatggcag agaacaaagg ttgaaataag caaaaaaatt
     8641 gaagacattg ctgaagaact tattgctatt caagcagcgc gttatcaaag accaggtttt
     8701 gcttttccgc ctgatgacga atttcaaaaa gaatttgaac ttggtttccc atatgaattg
     8761 acctacgacc aagaaatagc gcttcgggaa attaaaaacg atatggaatc accacttatt
     8821 atggaccgac ttctttcggg tgatgtaggc tttggtaaaa cagaaatagc acttcgcgcc
     8881 gcctttaaag caataagcgc tggtaaacaa gtaatgtttt tatgccccac aacgttactt
     8941 gcaagacagc actatgaggt gtcactagag agatttgcta atttcggtgt aaaaatagca
     9001 atgatttctc gtcttgttac tcaaaaagaa aaggatgaaa tcgtaaaaga ctttcaagct
     9061 ggcaatattc atttgcttat tggtacacat gctttgttta atatagatgt tgaaagcgtt
     9121 gatcttggat tattaattgt cgatgaagaa caaaggtttg gcgttgaaca aaaagagatt
     9181 attaaaaaaa tgaaaacaag agtcgatgtt ttaacattga gcgccactcc tattccgcgt
     9241 acattacaaa tgtcattagt tggcatccgc ggcttaagtc aaattaatac tgctccccaa
     9301 gaaagaatgc caattcaaac ttatgtcatt aaaagagatg attatgtaat caaagaatta
     9361 gtggagcgcg agttagggcg tggcggacaa gtattttatt tatataatcg tattgcaacg
     9421 atataccatg tcgcaaataa gttaagcagg ctagtgccac aagctaaaat tggcgttgta
     9481 catgggaaaa tggatcgcgc tgatattgaa gatgtaatga caagtttcta taataacgaa
     9541 attaatgttt tagtcgctac ttctattatt gaaaacggga ttgatgtgcc aaatgcaaat
     9601 gtaatcatcg ttgaaaatgc tgatactttt ggactatcgc aactttatca aataaaagga
     9661 agggttggaa gaggaaaccg cattgcatac gcatatttac tttatcaagg caacaaaata
     9721 atgaatgatg atgcgaaaaa aagacttcaa gcaattcaag agtttacaga tttaggcagt
     9781 ggctacaaaa tagcacaacg cgatttaatg attcgtggtg ccggtgatat tttaggtgcg
     9841 gaacaatctg ggtttattga tagtatcggt attgatatgt atttaaagat gcttgaagat
     9901 gcaataaatg agaaaaaata taaactaaca agtgatccaa tagaaaaaac aatcaatatt
     9961 agcggattag aagcttatat tcctgatgat tttgctagta agtcagaaaa atttgatatt
    10021 tatctcgaaa ttaaaaaaag taaaaagatg aaagaactaa aagactttga agaaaaagta
    10081 atcgatatgt atggtaaatt accacgttca ttagaaatct tatttttaaa acgccaagtt
    10141 gaactagtgg taaatagtcg cgctaacttt attgaagaca ttaaagatga agatgtattt
    10201 attgatatta ttccaggaag agcattttta gatatccctg gctcagtagt aatgttaaat
    10261 aatgaaatag ggacaacata ccagaacatt cgttttataa cccaagatga taaaattaag
    10321 ttaaggataa ttaaaagagg agattggttt cacgcatacg cgtcaattgt taaagcaatt
    10381 gtcatgattg ttcgtgaaac aatgaaaaat aaagatgaga ctagataaat ttctaaaatt
    10441 gacgcgatta attaaacgaa gaacggtggc aaaagatata agtacggccg gaaaagttat
    10501 cgttgataaa agaactgtta aaccatcata cactgttaaa gttgatgatg ttttagaatt
    10561 atatttagga gcacatatcg ttgtcgtaaa agtacttacg gttgatgaaa aagaaataaa
    10621 aagtaatcca gaaaaaggat atgaattatt aagtagaaca aaaaatgcaa attaatcttg
    10681 atcataacaa aaaatactta ttagcgtgca gctatggtcc tgattctatg gcgctttttt
    10741 cactattgct aaaaggaaac tataactttt cagttgcgca tattaattac ggattaagag
    10801 gcgaagagag tgaaaaagag actaacgatc tcgcacttta ttgcaaagaa cataatattg
    10861 ctttttttgt aaagtgggtt aacggtaaaa aaatcaaagg taacttccaa aacgaagcaa
    10921 gaaaagttcg ttatgagttt tttgaagaga ttattaaaga acacgatttt gatgcactat
    10981 taacagcgca tcacgctgat gacgtgattg aaacatattt aatgcagcaa aatagtaaac
    11041 gtgaaacatt ttactatgga attagagaag aaattgttct aaacaacttt cttgttattc
    11101 gtccgttact aaactattac aaaaatgatc tagtgtttta ttgtgtcgaa cacaaagttc
    11161 cttttgcagt agacagttca aatttagagt taacatacac cagaaataaa attcgccaca
    11221 acttagttaa aaaaatgtct gttgaacaaa aagacaaact actcctagac attaaagggc
    11281 gcaataaaga tttaaaagca atgacaaata aacttgaatt attgttagaa aatagtacta
    11341 tttccataac ggaatttatt aaactaaata atagggagca acagcatctt ttatatatgc
    11401 tttttcataa taaaggaatt gcagataagt ttagtaaggg cagaaataac cagattaaag
    11461 atgttgtgtt aagtgataaa aattcagcaa tgattaaagt aattgattca tattatttag
    11521 tgaagtatga agataaattt tcattaatta atatcaataa ttatcagcct tacaaatata
    11581 ttgttgaaac accagaaaca attaaaaccc cgcaattcgt ggcttttttt ggcaaaaaca
    11641 atgtatttcc taatattcct gctagcgaat atcctttgac aattgctaca caaggaaaaa
    11701 atcaaaagta taaaatcaaa ggttatgaaa agacaataaa tcgtttattt attgatatga
    11761 agatgccgcg tcactatcga cttatttggc cggttgttac aaataaaaat ggtgaagtaa
    11821 tttatgtccc gcgctatcgc gctaactaca ttccaaaagc aacggattta tttaaaattt
    11881 atattaaata aaacgcaaat gttgaaatct aagattatac tctgtataat aactttcagt
    11941 gtttatttat gttacacata ttcgaaatta atctataacc caaggaggtc ttaatgagca
    12001 atccaaatcc acgtccaaac cctagaagcg ttccaccgcg tcgctcgctg cgaacccttt
    12061 taccttattt acttttattt gctgtaatcg gtgttgttat ctggttagca ttttttacgg
    12121 gaaacgctaa aactcaagca ctacttcaaa ccgaactagc agcatactta caaacaaatg
    12181 ctggtgttat tgacacaatt actattgtaa ctaccgaaac caccacgaaa attactggta
    12241 ttttaaaaga cggtagtaag tttaccgctt tagtagaccg aacattttat gaaaacatct
    12301 tacaaccaac attattcgcc gctgatggaa cggcgcttgt tgactttaag atggttattg
    12361 ttaatgctta tcaacgcaca ttttggaaca ctattggacc aagcttactt tatcttgtcc
    12421 tatttgtagt ggcaatgata ttcctttact caagaatggc tggtgcagtt agcagttcaa
    12481 atactaaagc catggaattt aatcgttcac gcgctcgctt agaaacaaaa gttaaaaaga
    12541 cgtttgacga tgttgcaggc gctgatgctg aaaaacaaga gatgatggaa attgttgatt
    12601 atttacgtaa tcccaaaaaa tatacaaaat tcggtgcaaa actaccaaaa ggtgtacttc
    12661 ttgtggggcc gccaggcacc ggtaaaacac ttttagctaa agcagtagca ggtgaggcta
    12721 atgtgccttt ctactcaatt tcaggttctg actttgttga aatgtttgtt ggtgttggtg
    12781 caggtcgcgt tagagacatg ttccaacaag caaagaaaac agcaccttgc ttaattttca
    12841 ttgatgaatt agacgctgtt ggtcgtcaac gtggtgccgg acttggtggt ggaaatgacg
    12901 aacgcgaaca aacgcttaac caacttttaa ttgagatgga tggttttagt gataactcag
    12961 gaattattgt tcttgcggcg acaaaccgcg atgacgtttt agacccagcc ttattacgtg
    13021 caggacggtt tgaccgtaaa attaccgtta gtctaccgga tcgcgctggt agaacagcaa
    13081 tctttggtgt tcatgctcgc ggtaaaatta ttgatgaatc aattgacttt gatgccattt
    13141 cacgccggac tgttggattt tcaggcgcag atattgaaag cgtgttaaat gatgcagcga
    13201 tcttagcaat tcgcgataat gctgacaaaa ttatgttaca gcatattgat gaagctattg
    13261 atcgtcgtat tgctggtcct gcccgcacaa gccgacaact taatgaacac gaacgcaaag
    13321 ttattgctta tcacgaagcg ggtcacgctg ttgtggggct aaatcaccca tttagtgata
    13381 aagtgcaaaa aattacaatt attccacgtg gtcaaactgg cggtcatgta cttatgactc
    13441 ctgaagaaga ccgcttctta atgactaaaa accaattaat tgctcgtatc gtcggttatt
    13501 taggcggtcg tacaagtgaa gaagtattct ttgatgatgt atcaacaggc gcacaaaatg
    13561 atattgaagt cgctacaaga attgctcgac tgatggttac tgaatttggt atgagtgatt
    13621 taggtccgat tcaatatgaa agagatacag gcagtgtctt tttaggacgc gattacactt
    13681 caagtcaaaa gaactttagc caacaagttg cgcttgaaat tgacaaagaa gtacgcaaga
    13741 ttattgaaca agcccatgat aaagctcggg aaattattga aaaacataaa gatgatgtta
    13801 ttcttattgc cgaaacatta cttgaccgcg aaacaattac ggcagaacaa attgatcgtt
    13861 tgttaaaaga tcgtaaactc gatgaaccaa aacaagcagt gattggtgaa gttgttttaa
    13921 ctgaaaaacc aaaagttgaa ccaacaccaa cacctgttcc ggaagacgat aaaaaataag
    13981 aaatcgaaag ccgaagcaat tcggcttttt aataacaatg tggaaaatag gaaacataga
    14041 aattaaaggt aaaaccattc ttgctccaat ggcgggcata acgacttttg cttatcgcga
    14101 atttatgcgc tccttcggtg tagcgctttg ttataccgaa atgataagtg atagcggcct
    14161 tatttttaat aatgaaaaaa catatgaata tttaccacga ccagaagaaa aaagaccaac
    14221 atcggtgcaa ctttttggcg gtgaaacaac taagctttta gtggcaattg aaaagattga
    14281 aaagcacacg gataattatg attttcttga tttaaattta ggatgtcccg tacctaaggt
    14341 aacacgaaat aatggcggtt ctgcatggtt aaagcatcca aacgagctta ttgctatggt
    14401 tgaaaaagtt gttaaaagaa gcacaaaacc agtgactgtt aaaattagaa ttgggtggga
    14461 tgaaaagcat attaatttca aagaactatt accaaaatta gaagaagcag gcgtgagttt
    14521 aattgccatt cacttgcgaa caacaaaaca actttatggc ggaaaagctc gctacgattt
    14581 agcgcaaaat attcaaaatt tagtaagtat accgctcgtt gtaagcgggg atatttatac
    14641 acttgatgat gcgataaagg cgcttgaaat aactggcgca caagcagtga tgctagcgcg
    14701 cggtcaactt ggaaatccgt ttttagcaac acagattgag acatacttta aaacgggaga
    14761 gagattaaaa gatccctcac tataccagca attagattat ttgtggacac tggcaaatat
    14821 gatggtcgaa gaaaaaggag aacacatggg aatcttagtc atgcgcggaa tcgctcatca
    14881 ctttattaaa gggtttccgt ttaccaaaac atacaaagtt aaactaacta aaatgtcgac
    14941 tttaaacgac ttagaattaa taattaagga gattaagaac caggctttgc ttatataagc
    15001 aaaaagtggt aaaatgtcca catctaaagg agttatttat gccaagagaa tttagtgatc
    15061 aagaattagt gagacgcgct aaagcggaag aacttagtgc gcaaggtatc gatccatttg
    15121 gaaaaaagta tgaacgaaca gatactagtc tttcaattaa agaaaaagct ggtgaattta
    15181 ctaaagaaga attagaagaa aaagctatta aagttaaaac agcgggacgc ataatgctaa
    15241 tgcgtattat gggtaaagct agttttttta cgatccaaga ccaagaagga agaattcaag
    15301 cctttattag taaacaaggt gttggtgaag aagtttacga cttatttaaa agtgctgata
    15361 ttggtgacat cgttggtgtt gaaggaacaa ttatgcgtac caaaacgggc gaagtcacag
    15421 tgaaggttga agtttatacc catattgtaa aagcattacg accacttccg gaaaagtttc
    15481 acggattaac tgatgttgaa gagcgttatc gtcgccgtta tgttgattta ataatgaatg
    15541 aatcatcaaa acatgttgcc ttaactcgtc cgaaaattat tcgtgcgatt caaaaattct
    15601 tagatgatcg tggatttgtt gaagttgaaa caagtatgtt aaatagtgta cttggtggcg
    15661 cgagcgctaa accatttatt actcatcata atacgcttaa tcgtgacttt tacctgcgaa
    15721 ttgccacgga attaccatta aaacgcttat tagtcggtgg cattgaaaaa gtatatgaaa
    15781 ttggtcgtct cttccgtaac gaagggatga gcacgcgtca taatccagaa tttacaacgg
    15841 ttgaagctta tcaagcttac gctgatttag atgatatgta tgaactttgc gaaagcctag
    15901 taagagaact tgcgattaaa gtcacaggtg atactaaatt accatggggc gatgaaatta
    15961 ttgatgttgg tgcgccgttc cgcaaagtat caatggttga tttggtcaaa gaaaagacag
    16021 gtgttgactt tagaaaaatt aaaacatttg aagaagcaaa agcaattgcg gataaacatg
    16081 aaattcatgt tgaaaagcac tggactggct cgggtcatat tcttaatgcc ttctttgaag
    16141 aattttgtga agcagactta attcaaccaa catttgtata tgggcatcca cttgaaattt
    16201 ccccactagc gaaaaaagcc gaagatccgc gttttacaga acgctttgaa ttatttatta
    16261 aagcaaacga actagcgaac gcctttactg aacttaatga tccgcgcgat caatatgaac
    16321 gctttaaagc acaattaaaa gcgcgagaac taggtgatga agaagctagt gaaatggacg
    16381 aagacttcgt tgaagcatta gaatacggga tgccaccggc aggcgggatt ggaatcggta
    16441 ttgatcgtct tgtaatgttt ttattaaatc aaacatcaat tcgggaagtt attcttttcc
    16501 caacaatgcg tgataataaa taaaataaaa ttgtaattat tactagcgaa caaggtgtgc
    16561 ctttacaaac gctagtaata gtaatataat tacaacgcta tattactgct tgtaatatac
    16621 tccatgctgt tggtaagcaa cagccgaaat agaccaaagg gaggtgtgct tatgaaaaag
    16681 tacgaaatca tgtatattct tcttgcagac ttaagcgaag aagaacgtaa agccgaaatg
    16741 gaaagtctcc atcaaatgat taccaattta ggcggtaaaa ttgttgatgt agataccgaa
    16801 aattggggct tacgtgattt tgcgtatccg attaatgatt tacttaaagg atattatgta
    16861 gtcattaatg tcgaaagtga tgctgcggca atcgctgaat tcgaacggtt atcaaaatta
    16921 aataaaaatg taattcgtca tcttgttatc gctaaataaa aacgaggagg aataacaatg
    16981 attaatcgag tagtcttagt gggaagactt acccacgacc cagaattacg taaaacatcg
    17041 tcaggtgcat ccgttaccaa cttcagcatc gcagtaaaca atcgtttcgc tcgcgaccaa
    17101 gaaaatagta caagtttctt tagagtctca tgctggaacc aaaccgcaga ctttgtcgct
    17161 caatacgcac gcaaaggaaa cttagtggga gttgatggac gcattgaaca acgttcttat
    17221 gtaaataatg ctggacaaaa agttaatacc gtagacatta ttgccgattc ggttcaatta
    17281 cttgagtcca aatctgaaag tagtgaagga accggttatc aaagtgatgc tgaacccgct
    17341 actgacttag gagaaggaat cgacatctct gatgatgatc ttccattcta aattattaaa
    17401 ggagaatagt ttattatgtc atatcgcaaa agtagaccac gacgtaaagt ttgctatttc
    17461 aaacaaaaca aaattactga aatagattat aaaaatgttg agcttttaaa acgttttgtt
    17521 actccaaacg gaaaaattac tccgcgtaga gttactggaa catctgcaaa gtatcaacgt
    17581 caacttgcag tcgcaattaa aaacgcacgc tttatgggat tacttccata ttacgtgagc
    17641 taaaaaaaga atgaagagag agcctcacga cagtgaggtt ctttatttta gttaattatg
    17701 aagaaaacaa aattttatta ttttgaatta ggcttagtaa tattaattac attagggctt
    17761 tatgtttttt ccactaattt tttattacta cattatattg gatttctagg tccagtaccg
    17821 cttgcggttt ttattattcg tcataaaatt aaagacggaa tatttcccgc catttttatg
    17881 atattaatgg gaacattaat tggacacttt ttacctgtgg gccaaggtag ttttatgcgt
    17941 ggtttcttat ttatgattta cgcaatgaca gtaggatttt tacacggcac gattagcaaa
    18001 acgaaaatta atcccttgat cgaaatatta attatcattg gcgtagaaat attttatggc
    18061 tttttattag tcatcgtttt ctatgttcaa aaagatccgc actttgctta tgatatccaa
    18121 tttggtcagt atctagaatt atttattaaa ctatttaaga tagatgaaac atcaaattac
    18181 gcgcaaaatg tcggtgttat tattaaaaat ggtgttattg cttacacaat tgtatttagt
    18241 tttataagtg ttttattaac gcatattctc attcacttaa cacgaaaatt tatctacaga
    18301 gaaaaaatgg aacacggccc attacacggt ttagagttta ctatttcgcg tgtcgcggca
    18361 ataacttatt tagttggttg tgtagtagtt ttagtactat ctttcctttt aacaaaggaa
    18421 ttaaatctgc taggaatttc tagtatcaac gcgatcttta ctgttttctt ttctgtcaca
    18481 attatttttg tgttccaagg tattttagcg gttaatcttc gccttgaggc taagggttgc
    18541 aataatctta gtacgctagt attcatcagt ggtatattac tttctcccat ctttgcaata
    18601 attggagcga ttaatccgct cttaccccgc aaagaaaaag cggttgtata atcattaaaa
    18661 taaaatcgct aataaacaaa ttgtttattc ataatcaaat ttatgctaaa ttgtatatat
    18721 aggaattaaa ttatggccag aaagaaaaag aatgatgttg ctttatatga tgactcttca
    18781 tacgaagaag atgatttaaa gtacgctgct gatgaagaag tttatgctga atcattagaa
    18841 gatgatgaag gcaaagcaaa aaagaaacgc aagaaaaaga aaaaagaacc aaaagacccc
    18901 aaagagcgga aggacggaag cgaaacgcgt ggcggtaaaa ctttaacaga ccgctatcat
    18961 tacagaggtc gatattatct tttacctgaa cgcgaattac gtaaacaact gcgcttctat
    19021 caaaaagtat ttaattatcg gtttttagga atcattgcag gtgtgattgc cttaatcttt
    19081 tctattcttt cgctgacatt taatgcttat tttgcagtct ttacactact tattgttatc
    19141 acgggactaa tctatggtct tttaggaatg cttaagttac gtggcctaaa ttggcttttt
    19201 gtcttaattg gaattggttt aaatatcatg gcgattgtat tagcgatgaa accattaaca
    19261 tttgtgttta aacatcttgg tgaaattatg gcgctcatta atgaatacat gagttctgcc
    19321 gggtcttaat tacactagtt atcaacatta aagtaataat tttcaacaaa atatatatta
    19381 tgggacatga aaggtattaa agataccttt tttattgtat aattactcta aagaaaaacg
    19441 aggtattgct atgatcaaag ctattactaa attacgcaaa cgaacgatta tccttgttat
    19501 agttgaaggg cttttactag gtttaattag tctcttctat tatttaaact tatttaattt
    19561 tcaagaaatc tttgtaccgc cagttatttt ttctgtcgcg ataggtgtcg ttttctttaa
    19621 tttgttcgtt tttattttca ttaccgcacg aataaataaa ttacggaaaa agaatacatt
    19681 tgaacttgta aacttattag gtggcgatgt taaagaggct tacaactttg gaatgctagg
    19741 tcttgttgtc gtcgataatg attttaatgt cacatgggcc agtgaattat ttaaagaacg
    19801 tcgtctttta attattgata aaaacattct tgcttggaag cctgaattaa ttcggcttgt
    19861 tgaactaagt gaagtgcttg cagataatgt tgaagacaca attaaagttg aaatcgaatc
    19921 acggaactac gaagttaaat atttaagcaa ggctggtatt ttcttgttta aagatattac
    19981 tgaatttgaa tatctctttg atagtaatca agcaacagca cctgttttag gcgtagtggt
    20041 tattgataac tttgtggata ttgttgttaa aggtgaacac tctaatccaa tttttgggac
    20101 agttaacagt ttaattctcg aatacttttc aaattatggg gtgctgctta aacaatatcg
    20161 tgaagatgct tatctactta ttttaaacaa agagaacttt gataagttat acgctgataa
    20221 tttttcaatt ttacaaaaaa tcaaagataa aacatcgaat caagattatc aaatttcgtt
    20281 atcaatggcg tttgcatata atttccctga tgttttaaaa ctaaatgaac tcgtccttaa
    20341 tgcgattgat acagcaatgg cgcgtggtgg tgaccaagtt gttattgccc gttacggtga
    20401 agaaagtgaa tttatcggtg gcgcaacttc acaagctgaa aaacgtaata aaacagcgat
    20461 taggttatat gcggatacaa ttttccggca tataagagaa gcaaaaaatg ttttaatcat
    20521 gacacacacc gatactgact ttgacgcttt gggtgcgtcg cttggtttaa aagctatggc
    20581 cgactatgta aagaaaacac aaaaaggcac aaaggttcaa tttcaacctg cacgcatagt
    20641 ttatgaacac aatttaactg aattaaaaac aagaatggct gtaaacagaa tgtttacgaa
    20701 acaagaaatg aatgatatgt ttatttcacc agaagaaatt gacgatcaaa aaggaaccgc
    20761 gagtctagtt aattccggca cacttttaat tattgctgat gtttcatgtc cacaattaac
    20821 gcttcatcca aaactccttg aacgcgtgga taaggtaatt gtgattgacc atcatcgtcg
    20881 cacagacgaa tttattgatc aaagacttta tgactatatt gaaacaagcg caagttcaac
    20941 ttgtgaaatt gtgactgaac tactacatta cggtaatact cctaacttcg tattaccacc
    21001 aaaatatgcg acctttatgc ttgctggaat ttatttagac acaaactact ttagaacaaa
    21061 aacaacagga tcacgtactt tcttagcaag tatggtgtta gaagattggg gcgctgatgt
    21121 tatgacagcg gacgatttct taaaggataa tgttgaagaa tatgcactta ttaccaaaat
    21181 tatgaatagc atgacaacac ttagtgttgg tattgtcttc ttaagagcag atgaaacaga
    21241 cattgttacc ccagaaactt tatcgaaagt tgcaaaccaa agcatgcgca ttaaaggtat
    21301 acaagcttcg tttgttattg gttacacggc gcctaaggtc gttaaagtca gcggtagaag
    21361 taacggtgaa attaatgttc aattcttact tgagaaagtt ggaggcggtg gtcaccaagc
    21421 aagcgctgca gctgttttta cggataagac attagatgat gtccaagaaa ttttagaaga
    21481 tgtaattaat caaaatctaa gtcaagccaa agtcgttggc ggcaagaagg gaggataaaa
    21541 tgaaagtaat tttattaaaa gacgttaaaa aggtcggaaa aaaggatgaa attgttgatg
    21601 ttagtgatgg ctacgccaca aactttttaa tcccacgcaa acttgctgtt ccgtataatg
    21661 aacggactat ttcccaatta aaagcaaatc aagcgcgtgc tttagccgaa gaaaaagcac
    21721 ataaagaaga agcgcttgtc aacaagaaaa aactagaaga aaaagaaatt agttttattc
    21781 tcgctattgg tgaaaaaggt aaagcttttg gggcgattac cgctaaaaaa gttgaagaaa
    21841 agattaaaga agaatttaaa attaatgttg atcgccgtaa gtttgtgaac tttcagccac
    21901 taaatcaatt aggacccaca atagtggaaa ttgaactttt taaagatgtc attgctaaag
    21961 taaaagttct agtcaaagat aaggagatta actattctta atcaagttaa atagtaaata
    22021 tatatcaaaa agat
//
LOCUS       DAOPCD010000004        15822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_3778_length_15822_cov_4.696962, whole genome shotgun sequence.
ACCESSION   DAOPCD010000004 DAOPCD010000000
VERSION     DAOPCD010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 15822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15822
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3778_length_15822_cov_4.696962"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLQ55_00330"
     CDS             complement(<1..447)
                     /locus_tag="PLQ55_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28422.1"
                     /translation="MKKPIFERITEEAKKEAALLVKETKSEASRILKAGKDAISAQNE
                     SELTEAKAKSVERVRQYKERNEKGLDNYKEQVRQEIVVSIFDEVVNKLANLEGEELLK
                     FVTNLIAKEKVKGDEVIHVSARNFKKFVTALSSKKDATNLDLLNKKD"
     gene            complement(457..930)
                     /locus_tag="PLQ55_00335"
     CDS             complement(457..930)
                     /locus_tag="PLQ55_00335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HPK28423.1"
                     /translation="MTTGLVYALIGAALAAGLAGVGSAVGVSLGGKAAAGVISEKPEL
                     FGRVLILQALPATQGIYGFLVAVLIMVKIGMIGGNPIALTNEQGLALFAAALPIAIGG
                     LASGIYQGKMAAASISMTGKNPAMSARGMTMTAMVETYAILALLVSVLMYIAVPV"
     gene            complement(931..2838)
                     /locus_tag="PLQ55_00340"
     CDS             complement(931..2838)
                     /locus_tag="PLQ55_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HPK28424.1"
                     /translation="MIVPVKKIRLLTAISEQGKLIEALQHESKFMLFEHENDPSLPSD
                     INNELRAIERGIKALSKYKKKKFFAYHEVKQSEFNTIEAEGKALLSKINSALKTIENA
                     EKEIAELQAEIKTLTPYLNLSVATKPLSELVRTRIVIGQVPLNLFEDFAKEADEKGHI
                     FEESSRDHEFVYGAFGYEVVYESQALILLNSYRFTEVTLPLYDTKLKALLPELNDNLA
                     NNDKAILEAKDSLKALSNDEEKLRIYYDYYYNLALRKLVVTKTTERVAYVEGWVREDE
                     FETLTKNLENKVDCDIEELAVQNEEMVPTATKNNKLVKPFESITNMFSVPNPNELDPN
                     PAMSVWYWIIFGIMMGDMGYGLALLIIFGLFLKLAKPKGGLKDLATIFLYSSIPSIIF
                     GILTGSFFGVSVDLLNIVGKWFGNPNLTSVVLQPVEDPLPMLIFSLALGIAHIITGLV
                     LKIINDARKRDIKSMLADAVSWILILVGLVIAVVVKNQIVLYVAYGMIGIGAIFILLF
                     SGRDKKGIIGKALAGLGGLYNVTSYLSDVLSYSRLLALSLSTAVIAFTMNLLAGMVGG
                     IPVVGILFAAIVYLIGHVFNFAMGLLSAYVHDGRLQYLEFFGKFYEGGGYEFTPFSYK
                     LKYVNAIKENE"
     gene            complement(2835..3143)
                     /locus_tag="PLQ55_00345"
     CDS             complement(2835..3143)
                     /locus_tag="PLQ55_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28425.1"
                     /translation="MSVLEKIVQAELDAKELLAKTREDNTKLLSDTHDELATLRAENA
                     LAIAREINALNKKADHEIEKLATTFAAKKETITNEIEKSAKANQSKLVDKVFKDIVEK
                     "
     gene            complement(3342..5783)
                     /locus_tag="PLQ55_00350"
     CDS             complement(3342..5783)
                     /locus_tag="PLQ55_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPK28426.1"
                     /translation="MEKNGQILVVSNNFNTKNAVKTALENDFILFFSTPSEMFNEVEK
                     HPELCGVIFELKDENIQSVEQFSRLYLKYPRLKLLVLASKEFYESIDEEARFGFWCME
                     TSTAADLLNKIYELKEYPLSTIEKISLVYAAFERAPFGIMIIPNDNLELALFNRKNDE
                     VLGYTGEEYKKLGLNKETHPDDLQRVAEIEKQAKLLGENSFNVTKRVIKKDGNIVWVE
                     QFISIIKSEKYEGFSRIVISWDVTKQKAVEDTLAESDRARATLLENLPGMAYRSLNDK
                     NWTMEFVSDGALALTGYRPEELIDNKTVSYYDLIVRVHRPRIRQAFDEATKSKKQFRQ
                     EYEIVTRNGTKKWVSETAKPVFDKKGNLLFYEGIITDINHMKKIEKQLKHFQQYDQKL
                     NLPNREYLIDKIQAKLDSKNYSGTIILVNLKESQRLYRSHGYQYMELLTASLVTKLKT
                     IETSRITLHYAEEDLFVFYAKYQMTREEIKKFYAELKSAIGHTISREQIRSGIGVTFL
                     HKSISIAEVYLQRARIASEFIDDENALISIHIYNDIMDQKVKREEHLRQELIAISYEP
                     GNEHKLRLMFQPIINLQTGKVRGFEALARFTSDKYGPIAPSEFIPIVENNRLIIAFGK
                     KIINLAVSFAKELLATGIHDCGIAINISVLQLLDPNFAQDLFARIESAGVPPEMIMIE
                     MTESIFSTNYEQLSEALAEIKDYGIDTAIDDFGTGFSSLARIERLNFNTVKMDRVFVT
                     PINAENAEQGIAQDIINICKKFNIQSLAEGIETKEQYDALKGLGCEFGQGYYMSRPLE
                     VDAAIEYIKNAKRKK"
     gene            complement(6039..6515)
                     /locus_tag="PLQ55_00355"
     CDS             complement(6039..6515)
                     /locus_tag="PLQ55_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009986389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HPK28427.1"
                     /translation="MNFNDFSHKDLRYYLAYGSNLNLAQMQERCGTSKKVGKAIIKDY
                     RLMFKGSENSNYLTIEKAKGYQVPVGVFIIQEGDEVALDRYEGFPTLYYKKDFVVELI
                     SENGKKTTINAFVYIMHEDRILGAPSEEYIRRVREGYKNFNFDEKILDEAIVFSAK"
     gene            complement(6540..7076)
                     /locus_tag="PLQ55_00360"
     CDS             complement(6540..7076)
                     /locus_tag="PLQ55_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPK28428.1"
                     /translation="MEKEVMVNGKTYTLIKLLGKGKGGYSYLATFNNKEVVVKMIHHE
                     PCEYYTFGNKIEAEYYDYQRLFKAGIRMPRLIDICKGQEIIIKEYVKGDTIFEYVKRD
                     VNIDLFIDQVREMAALAKKAGLNIDYYPTNFVVQDNLIYYVDYECNEFSEEWNFENWG
                     IKYWTRTKEFKAVLKKEQ"
     gene            7161..8639
                     /locus_tag="PLQ55_00365"
     CDS             7161..8639
                     /locus_tag="PLQ55_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HPK28429.1"
                     /translation="MKIISKSNYTLFKNCPKSLWLSFYKSDVAAPLTMDVLKRIEDGK
                     EVGDLAKKYFANTIDVKSLLPDGSLDISKMLELTKKYLKQKGVTIAEASFKVNNLFCS
                     IDLLRVTSEGYEIYEVKSTTEAKVEHKVDAAFQKYVLQQYGLNVINTYILHLNKDYIR
                     KGDLELDQLFTMNHLDEDTSFIEAMHNMKSDLKAIDKLLLTKAEPAETLLTRCKNCDF
                     KDYCQKDIPVPSVLDVYRLSGYKYLNKGIVTFEDLLESNVALRKRQSIQVHAYLDNKE
                     VIIDKKGLASFLKKIKYPVYHLDFESYQMPIPPCNLMKPYDQIPTQYSLHIEYEDGSL
                     EHKAFLGNSLDPRREIAESLCANIPRNACVTAYYKGFECGRLNELATLFPDLEEHLLN
                     ISKNVIDLLEPFESGYYYHKNMGKSNSIKAVLPALYPDDSELDYSALPVVHNGSEAMV
                     MYPIMLGAKKEEKERIRNGLLEYCRLDTLAMVKLLQKLRKTQ"
     gene            complement(8643..9554)
                     /locus_tag="PLQ55_00370"
     CDS             complement(8643..9554)
                     /locus_tag="PLQ55_00370"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HPK28430.1"
                     /translation="MKKVFVLGSINYDVVVYTDKMPILGESIIGQGMIANLGGKGANH
                     AIAAKKIGVDKVFLISAVGDDDSGHIMLKSIKEHGVDTSGIEVIKGEPSGTCFIIFNR
                     SAEDNAVIVGNGANLMNAPQKAIDLLKKYARKGDIFIAQLETNLEALYKGIDFAHELG
                     MYIIINPSPVCEFNKSILSKVDLLILNAAEAKLLTGVNYKNNNDLQEIHDKLKAKSTI
                     VTFGEDGAYLIRDNEIIYQLAIPTNVVDTTCAGDTFLGALAYRKANGYEIEDSLRFAA
                     TASSIAVSRKGATQSVPYLNEILELYK"
     gene            complement(9585..10631)
                     /locus_tag="PLQ55_00375"
     CDS             complement(9585..10631)
                     /locus_tag="PLQ55_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPK28431.1"
                     /translation="MRKFDYLFLKDGSVPASLIGLISSIYSLRTLDETRKHKYASIYT
                     KLIKIAKVQSVKGSNAIEGIVTSDERIKAIVNQNSAPLNHDEEEIAGYRDALNIIHNT
                     YENLQFEEKTILDLHRLLLAVSSPENAGRYKTSDNVIIEIKPDGTRNVRFRPTPTSET
                     APAIEQLILAYTIAVAESGINKLLLIPCVILDFLCIHPFSDGNGRVSRLLTLLLLYKA
                     GFDAAKYISFEEQINKRKGFYYEALRKSSVGWHENNNNYFFFIENFLVTLFYCYQELD
                     KRFLTVNSEKVNKTARIEATVLNSIVPISKREICEILPDISVTTVEYVLGKMVKSGKV
                     RKIGKSSNTKYFRM"
     gene            complement(11590..12306)
                     /locus_tag="PLQ55_00380"
                     /pseudo
     CDS             complement(11590..12306)
                     /locus_tag="PLQ55_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(12432..13361)
                     /locus_tag="PLQ55_00385"
     CDS             complement(12432..13361)
                     /locus_tag="PLQ55_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HPK28432.1"
                     /translation="MVKNKVAVIIDTDLGADCDDVGTLVLALQLEKHGFCDIKAVGYC
                     TTNIYGVQCVDIVRSFYGGKFPIGMYYGKKVECNEDQHIFDIDLVDHYKDLIKETNYI
                     EAYKLYRKVLAEAEDDSIIFITIGELTNAANLVSSLPDEISPLSGQELFAKKVKYSCV
                     MGGLINDNHPITAFNNNEAFPEYNIVCAIEDAQKFVKISAKPIYFLDFYVGVDVFTGK
                     KVIEHDEHSPMKFAYLKYANGPSRSWDLLAVYYAITKRLYRSVGPGVMHIDDNGFTTF
                     TKDQDGHHYYLRPIKSNEEITKEIDEALVGNIL"
     gene            complement(13348..14052)
                     /locus_tag="PLQ55_00390"
     CDS             complement(13348..14052)
                     /locus_tag="PLQ55_00390"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPK28433.1"
                     /translation="MFPNNTHKAITFSFDDGVTQDRRLVALFNKYNLKCTFNLNSGGF
                     GKDGVCHWQKWSAPHNKVNSAEVKALYSGHEVAVHTVNHPDLTTIADEKIIIDEIEQD
                     RKVLEKLTGDKIVGMAYPFGTLNDRIVNIIKRNTPIKYARSIWATYNFDLQDNLLAFR
                     PTIHFLDFKKMYELAGTFLNTEYYEDKIFYIWGHSYELDFEDAWEEFEKFLAYIANKK
                     DVFYGTNKEVLLNGQK"
     gene            complement(14054..14941)
                     /locus_tag="PLQ55_00395"
     CDS             complement(14054..14941)
                     /locus_tag="PLQ55_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28434.1"
                     /translation="MSNKGKIKLKANTAYKISFDTKGYRVGGNGAYYFLKLRSGQENI
                     MTIGEWYDRPDAPANNKIFTFTTPENSKDLTIVFGIKNEGGYYVDNISLIELPNGHIV
                     DGEDVGFDNNVRPFTPNKGLGYEEGFEDGVLADSLFSFGFNRWGHFTNKPNEVINEKL
                     SFTSELDEVTYQFQDKNNYYEFMYSNDKYLKLSGNSTYRLTLKYKLMTEIRLHSDPSL
                     KGYAYIFARSKATHVDGNPDNPLLVGTEIHFASASTLETVYTETFELMTPSCDDTMII
                     IGLYGIGTMIVDDILVEKV"
     gene            complement(15172..15567)
                     /locus_tag="PLQ55_00400"
     CDS             complement(15172..15567)
                     /locus_tag="PLQ55_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28435.1"
                     /translation="MFFNHLPLTYTAGKNYRNVIKFLEAPTTTEMYINMVGNWAGHTT
                     FTINPVTGEVTHDSGLETCDITWSDNTLVIDFYCSTTLPEQQIRFVSKSIAGTVSDFK
                     IAEFTVYDLDLLTAKAFAKDLLNKTANTT"
     gene            complement(15624..15822)
                     /locus_tag="PLQ55_00405"
                     /pseudo
     CDS             complement(15624..15822)
                     /locus_tag="PLQ55_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
BASE COUNT         4801 a         3113 c         2369 g         5539 t
ORIGIN      
        1 atctttctta tttaataagt caaggtttgt cgcatctttc ttacttgaaa gcgcggtcac
       61 aaatttctta aagttgcgcg cagaaacatg tattacttca tcacctttaa ctttttcttt
      121 agcaattaaa ttagtgacaa attttaataa ttcttcacct tctaaattcg ctaatttatt
      181 aacaacctcg tcaaagatgc ttacgacaat ttcttgacga acttgttctt tatagttgtc
      241 cagtcctttt tcgttacgct ctttatattg gcgaacgcgt tcgacacttt ttgctttagc
      301 ttccgttaat tcggactcat tttgcgcgga aatagcgtcc tttcccgctt ttaaaatacg
      361 gctggcctcg cttttagttt ccttaactaa aagtgcagcc tcctttttag cttcttcagt
      421 aattcgttca aagattggct ttttcataag cgctccttaa accggtaccg caatgtacat
      481 taagacacta acaagtaacg ctaaaattgc gtaagtttca accatcgctg tcattgtcat
      541 accacgcgca gacattgctg ggtttttacc agtcatgcta attgaagcag cagccatctt
      601 accttgataa atgcctgaag ctagcccacc aatcgcaatc ggtaaagcag cggcaaataa
      661 tgctaaacct tgttcattag taagagcaat cgggtttcca ccaatcatcc caattttaac
      721 cataattaag acggcgacga ggaacccgta aatcccttgt gtagctggta aggcttgtaa
      781 aattaacacc cgaccaaata attctggttt ttctgagatt acaccagcgg cggctttacc
      841 acctaaagaa acacctactg ctgaaccaac gcccgcaaga cctgcggcaa gagctgctcc
      901 aatgagcgca tacactaatc ctgttgtcat ttattcattc tcctttattg cattaacata
      961 ttttaatttg tacgaaaacg gcgtaaattc ataaccgcca ccttcgtaga acttcccaaa
     1021 aaattctaag tattgtaacc gtccatcatg gacataggca cttagaaggc ccattgcaaa
     1081 gttaaagaca tggccaatta aatacacaat cgccgcaaat aaaatgccga cgactggaat
     1141 accgccaacc atgcccgcta aaagattcat cgtaaaagca ataactgctg tacttaaact
     1201 aagcgctaat aaccgtgaat aacttaaaac atcacttaag taacttgtaa cattgtataa
     1261 tccgcctaga ccagcaagcg ctttaccaat gatccctttc ttatcgcgtc cgctaaataa
     1321 taagataaaa atagcgccaa tcccaatcat gccgtaagct acatagagca caatttgatt
     1381 ttttacaaca accgctatca ctaatcccac taagattaaa atccaactaa cagcgtcagc
     1441 aagcattgat ttaatatcac gctttctcgc atcattaata atctttagta ctaaacccgt
     1501 aataatatga gcaattccta acgctagtga aaagattaac atcggcagcg gatcttcaac
     1561 gggttgcagt actactgaag ttaagtttgg atttccaaac catttaccaa caatatttaa
     1621 taaatcgaca gaaacaccaa agaaactgcc agtaagtatc ccgaaaataa tcgagggaat
     1681 acttgaataa agaaagatag ttgctaagtc tttaagtccg cctttaggtt ttgctagttt
     1741 taaaaataac ccaaaaataa ttagcagcgc tagaccataa cccatatcac ccatcataat
     1801 cccgaaaata atccaatacc acactgacat tgcggggtta ggatcaagtt cattagggtt
     1861 aggaacacta aacatgtttg tgatcgattc gaatggtttc actagcttat tattttttgt
     1921 cgcagttggc accatttctt cgttttgcac tgctaactct tcaatatcac aatcaacttt
     1981 attttctaag ttttttgtta aggtttcaaa ctcatcttca cgcacccaac cttcaacata
     2041 agcgacgcgt tcagtcgttt tagttaccac gagtttacgc agtgctaaat tgtaataata
     2101 gtcgtaataa atccgtaact tttcttcatc gttacttaac gcttttaaac tatcttttgc
     2161 ttctaaaata gctttgtcgt tattcgctaa attgtcattt aattccggca aaagtgcttt
     2221 aagttttgta tcatatagtg gtagtgttac ttcagtaaaa cgatatgaat taagtaaaat
     2281 gagcgcttgg ctttcataca ctacttcata accaaaagcg ccatagacaa attcatggtc
     2341 gcgtgatgac tcttcaaaaa tatgaccttt ttcgtccgct tctttagcaa aatcttcaaa
     2401 taaattaagc gggacttgtc caataacaat ccgtgttcgc actagttcac taagtggttt
     2461 agtggcaaca cttaaattta agtacggcgt taatgtttta atttctgctt gtaattccgc
     2521 aatttctttt tcagcgtttt caattgtttt taaagcgcta ttaattttag aaagcaaagc
     2581 tttaccttcc gcttcaatcg tgttaaattc actttgttta acttcatgat aggcaaaaaa
     2641 ctttttcttt ttgtatttgc taagcgcttt aatcccgcgc tcaatcgctc gaagttcatt
     2701 attaatatca ctaggaaggc ttggatcgtt ttcatgttca aaaagcataa acttgctttc
     2761 gtgttgtaat gcttcaataa gcttaccttg ttcactgatt gcggttaata gtctaatttt
     2821 ctttactgga acaatcattt ttctacaatg tccttaaaga ctttatccac tagtttactt
     2881 tggtttgctt ttgcactttt ttcaatctca ttagtgattg tttctttttt cgcagcaaaa
     2941 gttgtcgcta gtttttcaat ctcgtgatca gcttttttat taagcgcgtt aatttcacgc
     3001 gcaattgcta aagcattttc agcgcgaagt gttgctaatt catcgtgagt atcacttaat
     3061 aatttcgtgt tatcttctct tgtcttagcg agtaattctt tcgcgtcaag ttcagcttgt
     3121 acaattttct ctaatacact cataggactc ctttctaggc attttgtccg ctaccaattt
     3181 atttatataa ataggtagag ttttgtatcc acgtgtagta ttttaacatg tttacattgc
     3241 taatgtaatg tgatacgact atgtgcttcc gctaaaatgt tgaatgtata ctaaaaaacc
     3301 gcactatgcg gttattttag ataagaacat aatagttaaa actatttttt gcgtttagcg
     3361 ttttttatat attcaattgc cgcgtcaact tcaagtgggc ggctcatata atacccttgc
     3421 ccgaattcac agcccaagcc ttttagggca tcatattgtt ctttagtttc aatgccttct
     3481 gcgagtgact gaatattaaa ctttttacaa atgttaataa tatcttgggc aataccttgc
     3541 tcagcgtttt cggcatttat tggtgtcaca aaaacgcggt ccattttaac cgtgttgaaa
     3601 ttcaaccttt cgattctcgc taatgaagaa aagccagtac caaagtcatc aattgccgta
     3661 tcaatcccgt aatctttaat ttcagcaagt gcttcactta attgttcata gtttgtagag
     3721 aaaattgatt cagtcatttc aatcataatc atttcaggcg ggacacccgc tgattcaatg
     3781 cgtgcaaaaa ggtcttgggc aaaatttgga tctaagagtt gtaatacgga aatattaatt
     3841 gcgataccac aatcatgaat ccctgttgcg agcaattctt ttgcaaagga aaccgctaag
     3901 ttaataattt ttttaccaaa agcaataata agacgattat tttcgacaat tggaataaat
     3961 tctgacgggg caataggccc atatttatca ctagtgaatc gcgcaagtgc ttcaaagcca
     4021 cgtactttac cagtttgcaa attaataatc ggttggaaca tgagtcgtaa tttatgttca
     4081 ttgcctggct cgtaagatat agcaattaac tcttgacgga ggtgttcttc tcgcttcact
     4141 ttttgatcca ttatatcgtt gtagatatgg atagaaataa gcgcattttc atcatcaata
     4201 aattcacttg caatccgtgc tctttgtaag tacacttccg caatgctaat gctcttatgt
     4261 aagaatgtta caccgatacc agagcgaatt tgttcgcgcg aaatagtgtg accaatcgca
     4321 cttttaagtt cagcgtaaaa ctttttaatt tcttcgcgtg tcatctgata tttagcgtaa
     4381 aatacaaaca aatcttcttc agcataatgc agtgttattc tgcttgtttc aattgttttt
     4441 aatttagtaa caagcgaagc tgttaataat tccatatatt gatagccatg tgaacgatat
     4501 aatctttgac tttcttttag gttcactaaa ataatcgttc cgctataatt tttactatct
     4561 aattttgctt gtattttatc aattaaatat tcgcggtttg gtaaattaag tttttgatca
     4621 tactgttgaa aatgtttgag ttgtttttca attttcttca tatgattaat gtcggtaatt
     4681 atcccttcat agaataataa attacctttt ttatcaaata ctggttttgc agtttcactt
     4741 acccattttt tagtgccatt cctcgtcact atttcatatt cttggcggaa ttgcttttta
     4801 ctctttgttg cttcatcaaa tgcttgacga attcttggtc tatgaacacg cacaattaaa
     4861 tcgtaatagg aaacagtctt attatcgatt aattcttcag gtcgataccc cgttaaggca
     4921 agcgctccat cagacacaaa ttccattgtc caatttttat cattaagtga gcgatacgcc
     4981 atacctggta aattttctaa taaagtagcg cgagcgcgat cactttccgc tagtgtgtct
     5041 tcaaccgctt tttgtttcgt gacatcccaa gatattacta tccgtgaaaa tccttcatat
     5101 ttttctgatt ttattatcga aataaattgt tcaacccata cgatgttacc gtcttttttt
     5161 ataacgcgtt tcgtgacatt gaaactattt tctccaagta gtttagcttg tttttcaatt
     5221 tccgctactc tttgcaaatc atcaggatga gtttccttat ttaatcccaa ctttttatat
     5281 tcttcgcctg tataaccaag aacctcatca ttttttcgat tgaaaagcgc tagttctaaa
     5341 ttatcgttgg ggataatcat gatcccaaac ggtgcacgtt caaaagcagc gtacaccagt
     5401 gatatttttt caattgtgga aagcggatat tcttttaatt catatatctt atttaataag
     5461 tccgcagcgg tgcttgtttc catacaccaa aagccaaaac gtgcctcttc atcaattgac
     5521 tcataaaatt ctttactcgc taacactaat agtttaaggc gaggatactt taaatataga
     5581 cgactaaact gctcaacgct ttgaatgttt tcgtctttca attcaaaaat aacgccacag
     5641 agttcagggt gtttttcaac ctcattaaac atctctgatg gcgtactaaa aaataatatg
     5701 aaatcattct caagcgcagt tttgactgcg ttcttggtat taaaattatt actgacaact
     5761 aaaatttgtc cgtttttttc catttgatcc cgtttctatt atacattata acatctgtta
     5821 tacatatttt tttgtttttt gaaaaaaata aatattattg agttttttcg gtgatttttc
     5881 gctccttaaa atttttaaaa atttaaaaaa attgcaatat aaaaattaat aaaatatata
     5941 aatgttttgc attttcttta gcaacatata ctcaaaaatc ataataatat taaaagtttt
     6001 atcttgcaaa acaatgttat ttcccttgca tttcgtggtt attttgcact aaatacaatt
     6061 gcttcatcta atattttttc gtcaaagtta aagtttttat atccttcgcg tactcttctt
     6121 atatattcct cactaggagc gccaagaatc ctgtcttcat gcatgatata gacaaaggca
     6181 ttaattgttg ttttctttcc gttttcactt attaattcaa caacaaaatc ttttttatag
     6241 taaagtgtcg gaaatccttc ataacggtca agcgctactt cgtcaccttc ttgaataata
     6301 aaaacaccaa ctggcacttg ataacccttt gctttttcaa tcgttaggta attgctattt
     6361 tcacttccct taaacatcaa gcgatagtct ttaattattg ctttaccaac ttttttcgat
     6421 gttccacacc gctcctgcat ttgtgcaaga ttaagattgc tgccgtaggc taaatagtac
     6481 cgcaaatctt tgtgactaaa atcattaaaa ttcataatat tttccccatt accttgtatt
     6541 tattgttctt tttttagcac tgctttaaat tccttcgtgc gtgtccaata tttaatgccc
     6601 caattttcaa aattccattc ttcgctgaac tcattacatt cgtaatcaac gtaatatatt
     6661 aagttgtctt gcacaacaaa attcgtgggg tagtaatcaa tatttaggcc cgcttttttg
     6721 gctaaagctg ccatttctcg aacttgatca ataaataaat cgatatttac atctcttttt
     6781 acatattcaa aaattgtgtc gcctttaaca tattctttaa tgataatctc ttggccttta
     6841 caaatatcaa ttaagcgcgg cattctaatg cccgccttga ataatcgttg ataatcgtaa
     6901 tattctgcct caatcttgtt gccaaaagta taatattcac atggttcatg atgaatcatt
     6961 ttaaccacga cttctttatt attaaaagtc gctaaatagg aataaccacc tttcccttta
     7021 ccgagcaatt ttattagtgt gtaagtttta ccgtttacca ttacttcttt ttccatagtt
     7081 caccatcagt atagcgtttt taacccttaa actaaagacc actatcgctt ttgaaggtga
     7141 aagctggtaa aataaataat atgaagatta tatcgaaaag taattacaca ctatttaaaa
     7201 attgtccgaa gtcactgtgg ttatcttttt ataaaagtga tgtcgcagcg cctttaacca
     7261 tggacgtttt aaaacgaatt gaagacggaa aagaggtcgg agaccttgct aaaaagtatt
     7321 ttgcaaacac aattgatgtt aagtctttat tgccagatgg ttcgctagac atttctaaaa
     7381 tgcttgaact aactaaaaaa tatttaaaac aaaaaggtgt cacaatcgct gaagcttctt
     7441 ttaaagtaaa taacctcttc tgttctatag acttattacg cgtcactagt gaaggctatg
     7501 aaatttatga agttaaatca acgaccgaag caaaagtaga acataaggtt gacgctgcat
     7561 ttcaaaagta tgtattacaa caatatggac taaatgttat taacacatat attctgcatt
     7621 taaataaaga ctatattcgt aaaggtgatt tagaattaga tcaactattt accatgaatc
     7681 atctagatga agacacatct ttcatagaag caatgcacaa tatgaaaagt gatttaaaag
     7741 ctattgataa attactgtta acaaaagctg aacccgctga aacattatta acaagatgta
     7801 aaaattgtga ctttaaagat tattgtcaaa aggatattcc tgttccaagc gtactagatg
     7861 tttaccgcct atcaggttac aaatatttaa ataaaggcat cgtcactttt gaagatttat
     7921 tagaaagcaa tgttgctttg cgaaaacgac aaagtattca agttcatgcc tatttagata
     7981 ataaagaagt cattatcgat aaaaaagggc ttgcctcttt tctaaagaaa attaagtatc
     8041 ctgtctatca tttagatttt gaatcctacc aaatgccaat cccaccatgt aaccttatga
     8101 agccatacga ccaaattccc acacaatatt cattacatat tgaatatgag gacggaagtt
     8161 tagaacataa agcctttctt ggtaattcac ttgatccgcg tcgcgaaatt gctgagtcat
     8221 tatgcgctaa tattccgcgt aatgcatgcg ttactgccta ttataaaggc tttgagtgtg
     8281 gtcgcttaaa tgaactcgcc acattatttc ctgatttaga agagcattta cttaacataa
     8341 gtaagaatgt catagatcta ttagaaccat tcgaaagcgg atattattac cataaaaaca
     8401 tgggtaaaag taactccatc aaggcggttt taccagcgct atatcctgat gattctgaac
     8461 ttgattatag tgcgctgccc gttgtccata acgggagcga ggctatggtt atgtatccaa
     8521 taatgctagg agcaaagaag gaagaaaaag aaagaatccg taacggactc ttagaatatt
     8581 gtcgcttaga tactttagca atggttaaac tcttgcaaaa attacgaaaa acacaataag
     8641 tgttatttat atagttcaag tatttcatta aggtagggca ctgattgggt tgcccctttt
     8701 cttgatactg caatacttga agcggtcgcg gcaaagcgta atgaatcttc tatttcgtaa
     8761 ccatttgctt tccgatacgc taatgcacct aaaaaagtat cacccgcaca tgtcgtgtcc
     8821 acaacatttg tgggtattgc cagctgataa attatttcgt tatctctaat taaatacgca
     8881 ccatcttcgc caaatgtaac aatcgttgac tttgctttta atttatcgtg tatttcctgc
     8941 aaatcgttgt tatttttata attaacccct gttaataact tggcttctgc cgcatttaaa
     9001 attaataaat ctactttcga caatatgctt ttattaaatt cacagactgg tgaaggatta
     9061 ataataatat acattcctaa ttcatgtgca aaatctattc ctttatatag cgcttctaaa
     9121 tttgtttcta attgtgcgat gaatatatcg cctttacgcg catatttctt taataagtca
     9181 attgcttttt ggggggcatt catcaaatta gcgccattcc cgacgatgac agcattatct
     9241 tccgcgctac gattaaaaat aataaaacat gtcccgctag gctcgccttt aattacttca
     9301 atcccgctag tgtctacacc atgttcttta atgcttttaa gcatgatatg accactatcg
     9361 tcatcaccaa ccgcactaat taaaaatact ttatcaacac caatcttttt cgccgcaatc
     9421 gcgtgattag cgcccttgcc ccctaaatta gcaatcattc cttgaccaat tattgattca
     9481 ccaagaatcg gcattttatc ggtatacact actacatcat agttaattga tcctaacacg
     9541 aatactttct tcattactta attgcctccg ttatttttta cacactacat tcggaaatat
     9601 ttagtgttag atgatttgcc aattttcctg actttaccac tcttaaccat ttttcctaaa
     9661 acatattcga cggtggtcac acttatgtct ggtaatattt cacatatctc acgttttgaa
     9721 atcggtacaa ttgaatttaa aaccgttgct tcaatccgcg cggtcttatt gactttttca
     9781 ctgtttacgg ttagaaacct tttatctaat tcttgataac aataaaagag cgttactaaa
     9841 aaattctcaa taaaaaagaa atagttatta ttattttcat gccagccaac cgaggatttc
     9901 ctaagtgctt cataataaaa tccttttctc ttattaattt gctcttcaaa agaaatatac
     9961 ttggcagcgt caaaaccagc tttataaagc aataataatg ttaagagtct agaaacccgt
    10021 ccattaccat cactaaatgg atgaatacac aagaaatcta atatcacgca cgggatcaac
    10081 aataatttat taatcccact ttcagcaacg gcaatagtgt aagcaagtat tagttgttca
    10141 attgcgggtg cggtttcact tgtcggagtc ggtctaaacc taacatttct tgttccatca
    10201 ggtttaattt ctattattac attatcacta gttttataac gacctgcatt ttcaggactt
    10261 gaaaccgcta aaagcaatcg atgtaaatct aatattgtct tttcttcaaa ttgtaaattt
    10321 tcatatgtat tatgaataat atttaatgca tcgcgatatc cagcaatttc ttcttcgtca
    10381 tgatttaagg gcgcgctatt ttgattaaca attgctttaa ttctttcgtc agaagtaaca
    10441 atcccttcaa tcgcattact acctttaacc gactgaactt tagcaatttt tataagcttt
    10501 gtataaattg atgcatattt atgctttctt gtttcatcaa gtgttcgtaa agaataaata
    10561 ctcgaaatta atccgattaa gctcgctgga acggacccgt cttttaaaaa caaataatca
    10621 aattttctca tactgcataa caacctttat tctctgcata tattttacta attattatgc
    10681 agagatgcaa ttattttatg tggtcgtctg cataattttg ataaattttt atgcagaaat
    10741 tttcttttac ataatttaag tattaatttt aaataatgaa agaccagatt taaaatcaaa
    10801 gaggctcatc ttattttcat caaatgttag tgtatgttct tctcccatta catatttatg
    10861 cttgccaaca ttctcttttg atttaaattt cataccatta aatttaaagt ataaataata
    10921 ttcataaccc aacaattcaa caaaatcgat tgttgctttt atttctggtg cttcattttc
    10981 gaaggtttca ccagcaatct ttggtttaat atcttctgga cggataccga attttatcgg
    11041 tagtcttttt ttcgttttac attccttata ataatcgata tttttcatat aatcagttat
    11101 ttcttgatta taaacttcaa tttcatcgtc tttttctatt ctttgccttg catgtttatc
    11161 ttctgttcct gcttcttttg caagcaaagt ttgataatta ctatttattg tttttaattc
    11221 agtgtctttg cgggtaagaa gcacatctaa ttttcttttc aagtaatcaa ttccttcttg
    11281 aataccatcg gtgtcaccat ccgctaaagc tttttctagg ttagaaatgt ttgtgcggtg
    11341 caaaccttct tttcgtgcat actttctttc aagtttaaac aaggagttaa ctttatagcg
    11401 ttcggctttt gaagacagat aaagttttcg cttgtctaat tttttggatg tttctagcaa
    11461 caactcttca tattctttta tctgtttatt gataaatgac atgactaaat tatttttatc
    11521 tttatctaat gagaatttgt aaccatcttc aaatttaata accccattat cataaatagc
    11581 gttaacagca ttcatcgcag gcgaaccaat aaatgttgct acaaataagt tgttcggatg
    11641 tgaataaact tcttctggtg tgccaatttg ttgaacacgc cctttatcca ttacgacaat
    11701 acgcgttgcc attgtcattg cttcagtttg gtcatgagtt acataaactg tcgttgctcc
    11761 aatccgatta tgaatcgaaa caatctcact gcgcatttga atccgtaatt tagcgtctaa
    11821 gtttgatagt ggttcatcca ttaaaaatac aggggaagct ttaacaatcg gtcttccaag
    11881 cgctactctt tgtcgttgtc caccacttag tgctgacggc tttcgatcta gatattcgtc
    11941 aatttctaat attcttgcag ctttttccac tctttcagca atcacatctt tttttagatt
    12001 ttgaatttct aagccaaatg ataggttttg gcggacagtc atatttgggt acaatgcata
    12061 actttgaaaa accattgata tgtttctatt tttaggttca atgtcattag cgtatttgcc
    12121 atcaatatat aaatcacccg cagttatttc ctctaagccc gcgatcattc ttagtgttgt
    12181 ggacttacca cagccagaag gaccgacaaa gacaataaat tcttgcggct ttatatctaa
    12241 cgaaaaatca aaaacagctt gaacaaaatt aggatatatt ttatttacat tttgtaagga
    12301 aatgatggca tctttcggtg caatatttag tttctttgat tcttcaaaac cgcgcgccgc
    12361 aacgttataa taatttagct ctcgtttttt atgttcaatt attttgtttc ttttttcatc
    12421 ttttgacttt ttcataatat gttccccact agtgcttcat ctatttcttt agtaatttct
    12481 tcattacttt taattggtct taaataataa tggtggccat cttgatcttt agtaaatgtc
    12541 gtgaatccgt tatcatcaat atgcattacc ccaggcccaa ccgatctata taagcgttta
    12601 gtaattgcat aataaaccgc taataaatcc caacttcgcg atggcccgtt agcgtatttt
    12661 aaatatgcaa atttcattgg tgaatgttca tcatgttcaa ttactttctt tcctgtgaaa
    12721 acatcgacgc caacataaaa atctaaaaaa tagatgggtt ttgccgagat tttaacaaac
    12781 ttttgtgcat cttcaatcgc acaaacaata ttatattcag ggaacgcttc attattatta
    12841 aaagcagtaa taggatgatt atcatttatt aatccaccca ttacacaact atactttact
    12901 tttttagcaa ataattcttg gccagataat ggtgatattt catcaggtaa agaacttacc
    12961 aaattcgctg cattagtgag ttcaccaatc gtgataaaaa taattgaatc atcttctgct
    13021 tcagcaagaa ctttacgata aagtttatat gcttcaatat aattagtttc ttttattaaa
    13081 tctttataat gatcgactaa atcaatatca aaaatatgtt ggtcttcgtt gcattcaact
    13141 tttttgccat aatacattcc aattggaaat tttccgccat agaaagaacg cacaatatca
    13201 acacattgca ccccataaat attcgtcgta caataaccga ctgccttaat atcgcaaaac
    13261 ccatgctttt ctaattgcaa cgctaacaca agggtcccaa catcatcaca atcagcgcct
    13321 aaatcagtat caataatgac agcaacttta tttttgacca tttagtaaca cctctttgtt
    13381 tgtcccgtaa aaaacatctt ttttattagc aatatacgct aggaattttt caaattcttc
    13441 ccacgcatct tcaaaatcca attcgtaaga atgcccccaa atataaaata tcttatcctc
    13501 ataatattca gtgttaagaa aagtgcctgc tagttcatac atctttttaa aatctaaaaa
    13561 atgaattgtt ggcctaaacg ctaataagtt atcttgtaaa tcaaaattgt atgtcgccca
    13621 aatacttctt gcgtatttaa tcggcgtatt tcttttaata atattaacta tcctatcatt
    13681 taatgtgcca aaaggatacg ccatcccaac tattttgtcg cctgttaatt tttccaacac
    13741 ctttcggtct tgttcaatct catcaatgat gattttttcg tcagcgattg ttgttaaatc
    13801 aggatggtta acagtgtgaa ccgctacttc gtggccgcta tataaagcct taacctctgc
    13861 gctatttacc ttgttatgtg gtgcactcca tttttgccag tgacagacac catctttccc
    13921 aaaaccacca ctatttagat tgaaagtgca ttttaaatta tacttattaa ataatgcaac
    13981 gaggcggcga tcttgtgtta ccccatcatc aaaggaaaaa gttatcgctt tatgtgtatt
    14041 atttggaaac atctcaaacc ttctccacta aaatatcatc aacaatcatt gtcccaatgc
    14101 cgtataagcc aataataatc attgtgtcat cgcaacttgg cgtcattagc tcaaaggtct
    14161 cggtataaac tgtttctaaa gtactggcgc tggcaaaatg aatttctgtt cccacaagta
    14221 acggattatc aggattaccg tcgacatgag tagccttgct tcgtgcaaag atataggcat
    14281 agccctttag tgaaggatca ctatgtagcc taatctccgt catcaattta tattttaaag
    14341 ttaaacgata agttgaattt ccgctaagtt ttaaatactt gtcatttgag tacatgaatt
    14401 catagtaatt attcttatct tggaattgat atgtaacctc gtctaattca cttgtaaatg
    14461 acaatttttc attaattacc tcattaggct tatttgtaaa gtgaccccat ctattaaaac
    14521 caaatgagaa taagctatct gctaaaacac catcttcaaa cccttcttca tagccaagac
    14581 ctttatttgg tgtaaatggt cgaacattat tatcaaagcc cacatcttcg ccatcaacaa
    14641 tatgaccatt tggtaattcg ataaggctaa tgttatcaac gtaatatccg ccctcatttt
    14701 taataccaaa gacaattgtt aaatccttac tgttttctgg cgtggtaaat gtaaatattt
    14761 tgttattcgc gggcgcatcg ggcctatcat accattcccc aattgtcatg atattttctt
    14821 gtccagagcg taatttaagg aaataataag caccgttgcc accaacgcga tatcctttag
    14881 tgtcaaagct tattttataa gcggtgttcg cttttaattt gatttttcct ttattactca
    14941 taagcccttg ttgctcacca tgcaaaacat tactttcgat taagacaaca tttgaaccat
    15001 taatgtcttt ttcttcatcg cgataaatat cagtataaat actgtctgta taccacgagt
    15061 cagtttcaat attatatttc ccttcaaaat taatgagact gctgctgacc ttgtttatct
    15121 taatattttt ataacttatt ttattttgat aaatacctga tcccgctatt tctaagtggt
    15181 atttgccgtc ttatttaata aatctttcgc aaacgccttg gcggttaata aatctaaatc
    15241 gtaaaccgta aattcagcaa tcttgaaatc agatactgta cccgcgatgc tttttgaaac
    15301 aaatcgaatt tgttgttcag gtaatgtcgt agaacagtag aaatcaatca ctaatgtgtt
    15361 atcactccac gtaatatcac aagtttctag tccgctatca tgggtaactt cacccgttac
    15421 tggattaatc gtaaatgttg tatgtccggc ccagttccca accatattaa tatacatttc
    15481 ggttgtggtt ggagcttcta aaaatttaat aacattccga tagtttttac ctgccgtata
    15541 tgttagtggt aagtgattaa agaacattgc ttcccatgta tcaacagtgt tgtttgtacc
    15601 atagaaatgt aaatattttt gtccacttgc tgcagtagca ggtcgccttt ggcgtgaccg
    15661 agcgaatcgt tgaggacctt gaagttgtcc agatcgatga acagcaccgc cacctgcttg
    15721 ccgccacgta cgcttgaagc gatcgcctgt tgcaggcggt caaccagcaa ccgccggttg
    15781 ggcaattgcg tcagcgtgtc gtaaaacgcc aggcgacgaa cc
//
LOCUS       DAOPCD010000005        12968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_4791_length_12968_cov_4.480833, whole genome shotgun sequence.
ACCESSION   DAOPCD010000005 DAOPCD010000000
VERSION     DAOPCD010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 12968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12968
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4791_length_12968_cov_4.480833"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..899
                     /locus_tag="PLQ55_00410"
     CDS             195..899
                     /locus_tag="PLQ55_00410"
                     /inference="COORDINATES: protein motif:HMM:NF025668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HPK28436.1"
                     /translation="MEVTNIGHIRGAEVIGIYTGKSRSLLYGPRKELRAFKKVYLDPG
                     EKQKINIFLPTESLKAYCPVKQEWVLENVNYNVYVGINVQTTDEIFQILVDDANDLSY
                     LKNYQKENEMYYRRADFNPSIKDFTTLFGGESLPLFFANRKRPFTLANNISDTTDYWW
                     GRTIYKIMAKTSRKLAGDDVVLQEMAVETIKELPFRSLSALSSGEVSTNQRDAILLLV
                     NGKPIRGIFKFLRKKK"
     gene            971..2260
                     /locus_tag="PLQ55_00415"
     CDS             971..2260
                     /locus_tag="PLQ55_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPK28437.1"
                     /translation="MSEEKVSTTQDLKVQKQTKRDRRYLINIIVILLITFGYTTYSLW
                     NELPAVINVFRGTEADYRMIIIIIVLIALKFFIEGIILFIFARLYTPTYKIHRGVANA
                     FIGQFYNDITPSATGGQFAQVKTFAQQGVPVSIGASIMVMHFILYQIVLVVFGGLLIL
                     LNLGEFARLGSVSIFKLNIPVWVFASFGFLANLVTIVGLFLLAYSRRVHHFVINVVVD
                     LLAKIKIVKRPEYRKNKLYTSTENFRIELRRLSSNVPASILIMVLFFLKFIVNYSVTY
                     FVALMLNPALYGGLSFWETISKTGFLGMITGFIPIPGAAGFAEYFYEIIFSPVFGGDA
                     AFTKAVQLIWRVATFYVNLIVGGFVTAFYRSSMEEIVDETGHVKTFEEVKSYTFAERR
                     ETSVTMYATSQLSIKEIQRRLSPKKKRRWRNHKEDQE"
     gene            2260..3429
                     /locus_tag="PLQ55_00420"
     CDS             2260..3429
                     /locus_tag="PLQ55_00420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK28438.1"
                     /translation="MRIAMFTDTFVPDTNGVAVSSYNLFKALNDRGHYCVVVTSNPFS
                     KEVTFIDNVLRIPGIEMKKLYGYNFAGPNRKAHKILKKLKIDIVHVQNEAGMGIFGKS
                     FARQQKIPYVYTYHTMYEDYTYYATKGRMETFAKAIIRRFSRWQAEIADEFIVPSDKT
                     KVSLREYGVQRYINVIPSGVDFSKFMYNPEDKPFLDEYKKEHGLDGTFNLVYVGRVAK
                     EKSIDVVIRGYALYVKKNLNHNTRLIIVGGGPAIPELEELAEKLQIAPFVHFVGKVLP
                     ADIGKYYHLGEVFVSASISETQGLTFMEAMAAERLLLCRFDEGLVGVIRDGESGFFFR
                     SEEDFGEMVKKIRSLSTKEIRNILDEANNIVDYYSNDRFVERCEHVYLRALRNSW"
     gene            3429..4199
                     /locus_tag="PLQ55_00425"
     CDS             3429..4199
                     /locus_tag="PLQ55_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HPK28439.1"
                     /translation="MVKIINITLKKEQYNITFDNGEELLVSAETIAEFFLYNGKELSL
                     KDLAKIKEYEAQTKHFSYASNLLARRPYTSHEIREKLRKRCVNEEVIDLIVDKLKRLN
                     LINDLRYALDKIQYLINVKKCSTRAIEQDLLRRGIHPYVIKDALAQSPRDERRELEIL
                     IPKLLKSYRNESLRRAYQKLKTKLYSLGFESDNIDEVLAKFNVEDYIDETENIKKALV
                     KLEKKAKGDTKVLYNKLVIAGYPASLIRKLLEDYSYEN"
     gene            4189..5139
                     /locus_tag="PLQ55_00430"
     CDS             4189..5139
                     /locus_tag="PLQ55_00430"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPK28440.1"
                     /translation="MKIKDLNDGIRVIENYLVTNVTKGVSNNGQMYLTVSLQDNTGQI
                     EARVWDANENDVEVFKQGNFVRVVADVIEYRSNLQLKVMTGEVLNPKEVRLDEFTMAS
                     PVKLETLTAELNRLLSSFADTDIQRLVERLISDRYEAFITYPAASRLHHEYTHGLLEH
                     TITIANLADRIIDYYLELYEGDWHINRDLVIAGILLHDLGKTIELSGPVLTSYTVAGR
                     LVGHISLMNAELLKVAEELEIKSEMPILLSHIILSHHGKLEYGSPVTPMTKEAQLVSM
                     IDDLDAKMKLIEKALNTTNGGEFTDRLWALDSTSFYKPKK"
     gene            complement(5140..5556)
                     /locus_tag="PLQ55_00435"
     CDS             complement(5140..5556)
                     /locus_tag="PLQ55_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPK28441.1"
                     /translation="MSKDCVFCKIIRREIPGAIVYEDNDVLAFLDISPTAPGHTLVIP
                     KEHHETFLGTPRKLMYNVMDVAQRIGQVQIKTLKAQGVNILINNYPPAGQSVPHFHVH
                     VIPRYGKKDGLKIAMVEHLDGDLNFPAVVEKIKSGL"
     gene            complement(5658..7220)
                     /locus_tag="PLQ55_00440"
     CDS             complement(5658..7220)
                     /locus_tag="PLQ55_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28442.1"
                     /translation="MKRKYLLGLAVAALLLTGCDKITAEPVDLDKTVLVDELSEVVNN
                     TLENVYKKYHDSANFKQFVLDEVLLTIAEHEFGPYDKLAADDPFKLAVDTRAKHYFYD
                     ELLSGSYTYRSIFDEKKYVVERVYGTENSYLVDANGNEVAIGAIDTLPFYKEGLFLPI
                     VNKDNFDDPAFKLVHIDYYKHYMQDRYVDKIYREKLTEKFVKEEQTSTLGRNYARKVE
                     YVAIKNNSNHPTAASALVNAFIDNNILGAEPADFNILANAWRGYEPDFIGNEAALLAA
                     TGLKTAEVNKTLIGDIESDFAKIKANPDLTDKAIEDRFTGSGKYPKEVGKQIEINEVR
                     KQSFVTKDWGIKDGGFSSLPEAIRSRLFNIGVANSVDFLKDDAGVLADAGKWEGDAEN
                     KKVASTFVRNIHDDYYLVPKTYEKGNNRNFLFFENDTFYIVKIVEAVNTAKLAAKDDD
                     LNSYEHFKTEAEIAEIVAHVTEALAKLDATKTNALNFYMKDLKIVFHDEDVYDFFKEK
                     FPDIFDDEKKKK"
     gene            complement(7225..8976)
                     /locus_tag="PLQ55_00445"
     CDS             complement(7225..8976)
                     /locus_tag="PLQ55_00445"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPK28443.1"
                     /translation="MRKPKRIIAGLALSALLLTGCSKDLVADKKGNIVTVTIDGNPVQ
                     INAGDMFEDYLKSSDGLKDYYDAVYEVVVRALFNKEEQTTERTEIYDIAALSVNDVKE
                     QAKNNAKGGGKYDDELKKILKTKNVETLKELEEKLAYDEMQVRLKKQFFDGGEGAWIT
                     NAMEELIVGERNQAGDLILDENNNPVYEGYLNNKLPYHVRHLLVKVGAANNDFVTGKI
                     SKDDANKLSSVIVALANHQDTFGLLANRYSEDEDSARQFGDLGIMNFTTNFVNEFKLG
                     VYAYDALYNADGVMTPEAKAAKLKIPGTAKTKLDALGLDEIPYGAALKLAAVREIEKS
                     DAGKEVNDGDAAFFPRNIIFNKYFNKHNIGVITPNEVNVLDEVGVEKAEFAALPGFKA
                     LPELGGKKVLTDEKGNVILVVRAGSAGSYEGIHFIVVERSALYDTVNGVTLKDYYTIE
                     TPGTAAFPKDDAGNDIDAFVNFLELDARGTKERIDKIRNEVKAFDPTINDRIFQKLVT
                     YLDVKFHDAELKAALDKYLANQYARSQFQLDQDLDKLWSEWIEYLDQQEVARTDRLVP
                     ETYIDDFKAGNDYNYGG"
     gene            complement(9241..10590)
                     /locus_tag="PLQ55_00450"
     CDS             complement(9241..10590)
                     /locus_tag="PLQ55_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPK28444.1"
                     /translation="MTGESIPKYLSVGDTAVGSTTNLSNTILIKVSAIGSDTVLAKII
                     KTVEETSLIKPKFQKAVDVIAKYFVFIILMIALVSFLVYQFILGKELSISLEVTTAVL
                     VVSCPCALGLATPTSIAVASGLAFKNKILYKGGEFFELANKIDAIAFDKTGTLTKGDL
                     IVSDYLGDIRYLSYTKGLEIHSTHPIGKSINNYRKDIIPFAIEEYEIIDGKGIKGTYL
                     QDQIIVGSATFIAEANISNPFLNEFEKFTGEGKVVIFTAINKKVVNMIILEDEIKAES
                     QTLITALKKMNINVYLITGDNENTAKYLANKVGIENYYSGVLPNEKAHIIKKLQEKHS
                     VVAFVGDGINDAPALKQADVSFSVSNASDIATDTADVTLLDNDLKLVLIAIDLSKKTT
                     ANIKQNIIWAFLYNLVMIPLAIFNIATPLVGGFLHVTSSLLVVFNALRLNLYKHKIK"
     gene            complement(10635..11366)
                     /locus_tag="PLQ55_00455"
     CDS             complement(10635..11366)
                     /locus_tag="PLQ55_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPK28445.1"
                     /translation="MKTTLIIPNIGCPNCAMAITEHFKTLNIEAKVNVNQKKVTFTYS
                     DKDSFTIILKELKAIGFPAAPIDYDEKRRRKYEKVRLIVATILVLPLAYTMFDNFGLN
                     IVPDIMLNGYFQMGFAAILQFIFGFRFYKNAFYQIKNKNLGMDVLIVLGTSVAFFYSL
                     YLTIFTDKTQLFFETSGMLIWMVTIGDYLEHLSQAKTTDTLKSLMALAVDEVRLIKNG
                     EEQIVSLNDVNVGDVIKVLAGEKSL"
     gene            11694..12506
                     /gene="sufC"
                     /locus_tag="PLQ55_00460"
     CDS             11694..12506
                     /gene="sufC"
                     /locus_tag="PLQ55_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPK28446.1"
                     /translation="MVLIEIKDLHASVNDIEILRGVNLKINRGEVVALLGPNGHGKST
                     LLQVIMGHPNYKVTKGEILYKGQDVLAMSVDERARAGLFLAMQNPSEVPGVQISDFLR
                     AAINTKREKPIKPIELFRVIDKASKEMQMPHDLASRNLNEGFSGGEKKRHEILQMKLL
                     KPEFAMLDEIDSGLDVDGIRIVSQQINEEKKDKTFLVISHYARLYELINPTRAIVMIN
                     GRVAIEGGMDVIKRIDQEGYEWIQVEHNINIEREDKRPIVLEICAVNERSKQ"
     gene            12503..>12968
                     /locus_tag="PLQ55_00465"
     CDS             12503..>12968
                     /locus_tag="PLQ55_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28447.1"
                     /translation="MSVFKALLINKMNINDDTLILSENDTVYHIFDENDLPTSVTKVI
                     LKSSKDIDFVVVLRQNKKFITYELAPYTRGKVIFMCSEENLSIDREIILLEGAEVKLI
                     MPDFHNGNRKVNIETKLSERKARAEWHLATYSQNIDKKVFNISFSHFGNESFA"
BASE COUNT         4109 a         2173 c         2442 g         4244 t
ORIGIN      
        1 aaaggattac ctggtggaga gtttctcact agtgatcaaa atgttttata tcgtaatcaa
       61 tttatgttgg ctatcgttat tacgatactt ttagagaaca ggtacttttc ccgtttgggc
      121 acgggttaag ttatacgcaa tttaacacta gccatcatga aattgaagaa gtaaaagacg
      181 gttataacat cacgatggaa gtgacgaata tcggtcatat ccgcggcgca gaagttattg
      241 gtatttacac aggaaagagc cgtagtttgc tctacggtcc tcgtaaagaa ttacgcgctt
      301 ttaagaaggt gtatcttgat ccaggtgaaa aacaaaagat caatatattc ttacctactg
      361 agtcattaaa agcttattgt ccagtaaagc aagagtgggt gcttgaaaat gtaaattaca
      421 atgtttatgt cggaataaat gtacaaacca ctgatgaaat cttccaaatc ttagtggatg
      481 acgctaatga tttaagctat ttaaaaaatt atcaaaaaga aaatgaaatg tattatcgtc
      541 gcgccgattt taatccatca attaaagatt ttacaacatt atttggtggt gaatctttac
      601 cattattttt cgctaacagg aagcgtccct tcacacttgc taataacatt agtgatacga
      661 ctgattactg gtggggacga acaatttata agattatggc caagacttca cgcaaactag
      721 cgggtgatga tgtagtactc caagagatgg ctgttgaaac gattaaagaa ttaccgttta
      781 gaagtctttc agccttaagt tctggagaag taagcactaa tcaacgagac gctattttac
      841 tattagtaaa tggcaaaccg attcgtggta ttttcaagtt tttacgaaaa aagaaataga
      901 aacatgtatg aatatacctt tcaagttgtc ttaactaagg acttaagtcc tttcatgcta
      961 taatttacct atgagcgaag agaaagttag cacaacccag gatttaaaag tacaaaagca
     1021 aacgaagcgt gatcgtcgct atcttattaa tatcatcgta attttattaa ttacttttgg
     1081 gtacactact tactcattat ggaatgaact cccagcggta ataaatgtgt tccgtggcac
     1141 ggaagctgat taccgcatga ttataataat tattgtttta attgctctaa agttttttat
     1201 tgaaggcatt atcttattta tttttgctag gctttatacc ccgacttata aaatacatcg
     1261 cggtgtcgcg aatgctttta ttggtcaatt ttataatgat attactccga gtgctacagg
     1321 tggtcaattt gcgcaggtaa aaacttttgc gcaacaagga gtgccagtat caattggagc
     1381 ttccattatg gtgatgcact ttattttgta tcaaattgtg cttgttgttt ttggtggatt
     1441 acttattctt ttaaaccttg gagaatttgc gcgacttggt tcggttagta tttttaaact
     1501 taatattcca gtgtgggttt ttgcttcatt tgggttttta gctaacttag ttacgatagt
     1561 tgggctattt ttactagcgt actcgagacg tgttcatcac tttgttatta atgttgtagt
     1621 cgatctttta gcgaaaatta aaattgttaa aagacctgaa tatcgtaaaa ataaacttta
     1681 tacttcaact gagaacttca gaattgagtt acggcgctta agttcaaacg tgccagcttc
     1741 aattcttatt atggttctct ttttcttaaa atttattgtt aactattcag ttacatattt
     1801 tgtggcttta atgttaaatc cagcattgta tggcggtctt tccttttggg aaacaatttc
     1861 taagactggt ttcttaggaa tgattacagg ctttattcca attcctggcg ctgctgggtt
     1921 tgccgaatat ttttacgaaa tcatcttctc ccctgttttt ggtggagatg ccgcctttac
     1981 gaaagcagtt cagcttatct ggcgggtggc aacattttat gttaacttaa tcgttggcgg
     2041 atttgtgacg gccttctatc gttcgagtat ggaagaaatt gttgatgaaa cgggtcacgt
     2101 taaaacattt gaagaagtta agagttacac ctttgctgaa cgtcgtgaaa ctagtgtgac
     2161 gatgtatgcc acaagtcaat tatcaattaa agaaattcag cgtcgtttat cacctaagaa
     2221 aaaacgaaga tggcgcaatc ataaagagga tcaagaataa tgcgaattgc aatgtttact
     2281 gatacatttg tccctgacac taatggggtc gccgtttcta gttataatct ttttaaagca
     2341 cttaacgatc gcggtcatta ctgtgttgtt gttacttcta atcctttttc caaagaagta
     2401 acatttatcg ataatgtttt gcggattccc ggaattgaaa tgaaaaaact ctatggttac
     2461 aactttgcgg gaccaaaccg taaagcacat aagattttaa aaaagcttaa aatcgatatc
     2521 gttcatgttc aaaatgaagc ggggatgggt atatttggaa aaagttttgc tcgtcaacaa
     2581 aagattcctt atgtgtatac atatcacaca atgtatgaag attatactta ttacgcgaca
     2641 aaaggacgga tggaaacatt tgctaaagct attatccgtc gcttttcacg atggcaagca
     2701 gaaattgctg atgaatttat tgtaccaagt gataaaacaa aggtgtcact tcgcgaatat
     2761 ggggttcagc gttatataaa tgttattccg tcgggtgtcg atttttcaaa atttatgtat
     2821 aatcctgaag ataaaccttt ccttgatgaa tataaaaagg aacacgggtt agacggcact
     2881 tttaacttag tgtatgttgg acgtgttgca aaagaaaaaa gtattgatgt cgtaattagg
     2941 ggttatgcac tttatgttaa aaagaatcta aatcataata ctagactcat catcgttggc
     3001 ggaggaccgg caattcctga attagaagaa cttgcagaaa aattacaaat tgctccgttt
     3061 gtccattttg tcggcaaagt cttacctgcg gatattggca agtattatca tttaggggaa
     3121 gtgtttgtta gtgcctcaat tagtgaaacc caaggcttaa cctttatgga agcgatggct
     3181 gcagaacgcc ttttactttg tcgttttgat gaagggcttg tcggggtgat tagagacggt
     3241 gaatcaggtt tctttttccg tagtgaagaa gactttggtg agatggtgaa gaaaattcgc
     3301 agtttatcca caaaagaaat tcgcaatata ttagatgaag caaacaatat agttgattat
     3361 tacagtaatg accgtttcgt agaaagatgc gagcacgtct atttaagggc ccttcgtaat
     3421 agttggtaat ggttaaaatt ataaatatca cattgaagaa agagcaatac aatatcactt
     3481 ttgataatgg tgaagaacta ttagtaagtg cagaaacaat tgcggagttt tttctttata
     3541 atggcaaaga gttatcacta aaagacttag cgaaaattaa agaatatgaa gcacagacta
     3601 agcattttag ttacgctagt aacttattag cgagaagacc ttacacaagt catgaaatac
     3661 gtgaaaaact ccgcaaaagg tgtgtaaatg aggaagttat tgatttaata gtcgataaac
     3721 ttaagcgtct taatttaatt aatgacttac ggtatgcact tgataaaata cagtacttaa
     3781 ttaatgttaa aaagtgctct actcgtgcta ttgagcaaga tttattaagg cgtggtattc
     3841 acccctatgt tattaaggat gctcttgcgc aatcgccacg tgacgagcga cgtgaacttg
     3901 aaatacttat acctaaatta ctaaaatcgt atcgtaatga atcactgcgc cgtgcttatc
     3961 aaaaacttaa aactaaatta tatagtctcg gctttgaaag tgataatatt gatgaagttt
     4021 tagcgaaatt taatgtagaa gattatatcg atgaaacaga aaatattaag aaagcactag
     4081 tgaaactaga aaagaaggca aaaggtgaca ctaaagtgtt gtataataaa ttagttatcg
     4141 cgggttatcc cgcttcctta attcgtaaat tgttggagga ttatagttat gaaaattaaa
     4201 gatttaaatg atggcattcg tgttattgaa aattatttag tgacgaatgt aactaaaggt
     4261 gtatcaaata acggtcaaat gtatctaacg gtcagtttgc aagataatac aggccaaatt
     4321 gaagcgcggg tatgggacgc aaatgaaaat gatgtagaag tatttaaaca aggtaacttt
     4381 gtgcgggttg tcgctgatgt tattgaatac cgttcgaatt tacaactgaa agtcatgacg
     4441 ggtgaagtcc taaatcctaa agaggttaga cttgatgaat ttacaatggc aagcccggtt
     4501 aaacttgaaa ctttaactgc ggaacttaat cgtttactct cttcatttgc ggacacggat
     4561 attcagcggc tcgtggaacg actaatcagt gatcgttatg aagcttttat aacttatcct
     4621 gccgcgagca gattacatca tgaatataca cacggtttgc ttgaacacac aattaccatc
     4681 gctaatcttg cagatcgtat cattgattat tatttagagt tgtatgaagg tgattggcat
     4741 attaatcgcg atttagttat tgcgggcatt ttactgcatg acttaggtaa aacaattgaa
     4801 ttaagtggac cagtgttaac tagttataca gttgctggta gacttgttgg ccatatttca
     4861 ctaatgaacg ctgaactttt aaaagttgca gaagaacttg aaattaaaag tgaaatgcca
     4921 attcttttat cacatatcat cttatcgcat cacggtaagc ttgaatacgg ttcacctgta
     4981 actccaatga caaaagaagc acaactcgtg agtatgattg atgacttaga tgccaaaatg
     5041 aaattaattg aaaaagcgct caacacaaca aacggcggtg aattcactga tcggttgtgg
     5101 gcgcttgatt caacgagttt ttataaaccg aaaaaataat tataaaccag acttaatttt
     5161 ttccacgact gctgggaagt ttaaatcgcc atcaaggtgt tcaaccatgg cgatttttaa
     5221 tccgtctttt ttaccataac gcgggattac atggacatga aagtgcggga cgctttgacc
     5281 agcgggcgga taattattaa ttaaaatatt aacgccttgc gctttaagtg ttttaatttg
     5341 cacttggccg atacgttgcg cgacatccat gacgttgtac attaatttac gcggagtgcc
     5401 taaaaatgtt tcgtggtgtt ctttaggaat aactaaagtg tgacccggag cggttgggga
     5461 aatatcaaga aaagcaagga catcattatc ttcatagacg atggcacctg ggatttctcg
     5521 gcgaattatc ttacaaaaaa cacaatcttt actcattagt gttcctcctt tatctatata
     5581 ttaattataa caaatatctg taaataaaaa ccgcaggggt taacctgcgg tcgttattta
     5641 ataaatagaa ggtatgctta tttcttcttt ttttcatcat cgaagatatc agggaatttt
     5701 tctttgaaga aatcatagac atcttcgtca tggaagacaa tctttaagtc tttcatgtag
     5761 aagttaagcg cattagtttt agtcgcatcg agtttcgcta aagcttcagt aacatgggca
     5821 acaatttccg caatttctgc ttcagttttg aaatgctcat atgaatttaa gtcatcatct
     5881 ttagcggcaa gtttagcggt gttaacggct tcaacgattt taacgatgta gaatgtatcg
     5941 ttttcgaaga agaggaagtt acgattgtta cctttttcat aagttttggg gactaggtaa
     6001 taatcgtcat gaatattacg aacgaaggtt gacgcaactt tcttgttttc ggcgtcgcct
     6061 tcccatttac cagcatcagc aaggacacca gcgtcgtctt ttaagaagtc aacactatta
     6121 gcgacaccaa tgttgaataa gcgggagcgg atagcttctg gtagactact aaagccacca
     6181 tctttaatac cccagtcttt cgtgacgaat gattgtttac gcacttcatt aatttcaatt
     6241 tgtttcccaa cttcttttgg atatttacca ctgccagtga aacgatcttc aatcgctttg
     6301 tcagttaagt cagggttagc tttaattttg gcgaaatcac tttcaatgtc accaattaag
     6361 gttttgttaa cttcagcggt tttaagtcca gttgcggcta gaagtgcagc ttcattacca
     6421 ataaagtctg gttcataacc tctccaagca ttagcaagga tattgaagtc agcaggctcg
     6481 gcgcctaaga tattattatc aataaaggca ttaacgagtg cactagcggc ggttgggtgg
     6541 ttactattat ttttaatagc gacatattca accttccggg catagttacg gccgagggtg
     6601 gaagtttgtt cttccttaac gaatttttct gttaattttt cgcgataaat cttatcaaca
     6661 tagcgatctt gcatgtaatg cttatagtaa tcgatatgga caagtttaaa ggctggatcg
     6721 tcaaagttat ctttgttgac gattggaagg aataagcctt ctttgtagaa tggtaaggta
     6781 tcaatcgcac caatggcgac ttcgttgccg ttagcgtcca ctaggtagct attttccgtt
     6841 ccgtatactc tttcaacgac gtatttcttt tcgtcaaaga ttgagcgata tgtgtatgaa
     6901 ccgcttaata gttcatcata gaaataatgt ttcgcgcgtg tatcgactgc gagtttgaat
     6961 gggtcgtctg cggctaattt gtcgtatgga ccaaattcat gttcggcaat cgtaagtaac
     7021 acttcgtcta atacgaattg tttaaagttc gcggagtcat gatatttctt atagacattt
     7081 tctaatgtgt tgttaacaac ttcgcttaat tcgtcgacaa gcacagtttt atctaaatca
     7141 actggttcgg cggtaatttt gtcacaacca gttaagagaa gtgcagcgac ggctaagcct
     7201 aataaatatt tacgtttcac tctattaacc tccgtaattg tagtcattac cagccttgaa
     7261 atcatcaata tatgtttcag gaacaaggcg gtcggtacgt gcgacttcct gttgatctaa
     7321 atattcaatc cattcgctcc ataatttatc aagatcttgg tcgagttgga attgggaacg
     7381 agcgtattgg ttcgctagat atttatcaag cgctgctttg agttcagcat catggaactt
     7441 cacatcaaga tatgtcacta acttttggaa aatgcgatcg ttgattgttg ggtcaaatgc
     7501 tttaacttca ttacgaatct tgtcgattct ttcttttgtg ccacgggcgt caagttctaa
     7561 gaagttgaca aaggcatcaa tatcgttacc agcatcgtct tttgggaaag cagcggttcc
     7621 tggggtttcg attgtgtagt agtcttttaa agtaacgccg tttacggtgt cataaagagc
     7681 actgcgttcg acaacgatga agtggattcc ttcataactt ccggctgaac cagcgcgcac
     7741 gactaagata acattacctt tttcgtcggt taaaactttt ttaccaccga gttctggaag
     7801 agctttaaat ccaggaaggg cagcaaattc agctttttct acaccgactt cgtcaaggac
     7861 attaacttca tttggagtaa tgactccaat gttatgttta ttaaagtatt tattaaagat
     7921 aatgttacgt gggaagaagg cggcatcacc gtcatttact tctttgccag catcgctctt
     7981 ttcgatttca cgaacagcgg cgagtttaag cgcagcgccg taaggaattt cgtcaagtcc
     8041 gagtgcgtct aatttagttt tggcggtgcc aggaatttta agtttagccg cttttgcttc
     8101 tggagtcata actccgtcag cgttgtaaag agcgtcataa gcataaactc ctaatttgaa
     8161 ctcattcaca aagtttgtgg tgaagttcat aatgcctaag tcaccaaatt gacgggcact
     8221 atcttcgtct tcactatagc ggtttgctaa aagtccgaag gtgtcttgat gatttgctaa
     8281 ggctacgata acagaactta atttgttcgc atcgtctttg gaaatcttac cggtaacaaa
     8341 gtcgttgttt gcggcaccga ctttaacgag taagtgacga acgtgatatg gaagtttgtt
     8401 gtttaagtat ccttcgtaaa caggattgtt gttttcatca agaattaaat ctcctgcttg
     8461 atttcgttca ccaacgatta attcttccat tgcgtttgtg atccaggcgc cttcgcctcc
     8521 atcaaagaat tgttttttga ggcggacttg catttcatcg tatgcgagtt tctcttctaa
     8581 ttcttttaat gtttcgacgt ttttagtctt aagaattttc ttaagttcat cgtcgtactt
     8641 gccaccacct ttggcgttat tctttgcttg ttctttcaca tcgttaacgc taagggcagc
     8701 aatatcatag atttcggttc gctctgtagt ttgctcttct ttattaaaga gagcgcgaac
     8761 cacaacttca tagacggcgt cgtaataatc ctttagtcca tcagaggatt ttaaatagtc
     8821 ttcaaacata tcccctgcat taatttgtac tgggttacca tcgattgtca ccgtcacaat
     8881 attgcctttt ttgtctgcga ctaaatcttt actacatccg gtaagaagta gtgccgatag
     8941 cgctaaacca gcaataatgc gttttggttt tctcatataa tgctatacct cctaataata
     9001 tgcttttata ttctatactt aacttccaac cttttcaaca aaaataagcg ttaatagtcg
     9061 ataaattaac caataactta tttttatata aaaatagaat acgaaaatat tgaatttttc
     9121 atgataattt aaggtttata caaatattat gaaaaagcaa aagagatatt tgtgtatttt
     9181 ctaaagtttg aattttcaaa aatgataatt aataaatcaa aattaatagg aacgaagata
     9241 ttattttatt ttgtgcttat ataaattgag acgcagtgca ttaaaaacga cgagaagact
     9301 agaagtaaca tggaggaaac cacctactag tggtgtagcg atattaaata ttgcaagcgg
     9361 aatcattact aagttatata aaaatgccca tattatattt tgtttaatat tggctgttgt
     9421 tttctttgat aaatcaatag caattaacac tagttttaaa tcgttatcta agagagttac
     9481 atctgctgta tcagtggcga tatcagaagc attactgaca gagaaactaa catccgcttg
     9541 ttttagggca ggtgcatcat taattccgtc accaacaaaa gcaactacac tatgcttttc
     9601 ttgtaatttt ttgatgatat gagctttttc attcggtaac acaccactat aataattctc
     9661 aattccaact ttatttgcta agtatttagc ggtattttca ttatcgccgg taattaggta
     9721 gacattgatg ttcatctttt taagtgctgt aattaaagtc tgactttcag cttttatttc
     9781 atcttccaaa ataatcatat ttacgacctt tttattaatc gcagtaaaga tgacgacctt
     9841 accttcacct gtaaattttt caaattcatt taagaaagga ttactaatat tagcttcagc
     9901 aataaaagta gcagaaccca ctataatttg atcttgtaaa tatgtgcctt taataccttt
     9961 gccatcaatt atttcatatt cctcaattgc aaaaggaata atatcttttc gatagttatt
    10021 gatagatttt ccgattggat gagtggaatg gatttctaag ccttttgtat aacttaaata
    10081 tctaatatcg ccaagataat cgctgactat taaatcacct ttagtaaggg ttcctgtttt
    10141 atcaaaagca atcgcatcaa ttttattggc gagttcaaaa aactcaccgc ctttatatag
    10201 aattttattt ttaaatgcta atccgcttgc aactgcaatt gaagttggtg ttgctaaccc
    10261 aagagcacaa ggacatgaaa ctaccaagac cgcagtagtc acctcgagag aaattgagag
    10321 ttctttccct aaaataaatt gataaacaag aaatgataca agggcaatca ttaaaataat
    10381 aaaaacgaaa tatttagcaa taacatccac agctttttgg aatttgggct taataagcga
    10441 agtttcttca accgttttaa taattttagc tagtacagta tcactgccga tagcacttac
    10501 tttaatcaaa attgtattag ataaattagt ggtagaccct acagcggtat cacctacact
    10561 aagatattta gggattgatt cgcctgttat aactgaatca tcaatataac tagcgccatc
    10621 aacaatttca ccatctaaag cgatttttcg ccggcaagca cttttataac atcgccaaca
    10681 ttcacatcat taagtgaaac tatttgttcc tctccgtttt ttataagcct aacttcatca
    10741 accgcaagtg ccattaatga ttttaatgta tccgttgttt ttgcttgtga gagatgttct
    10801 aaataatcac caattgttac catccaaata agcataccgc tcgtttcaaa aaacaattga
    10861 gttttatctg taaaaatagt taaatacaaa ctataaaaga acgctactga agtacctaaa
    10921 acgattaaaa catccattcc taaattttta ttttttattt gataaaaagc atttttataa
    10981 aatctaaatc caaaaataaa ttgcaatatt gcagcaaaac ccatttggaa atagccgttt
    11041 agcataatat cggggacaat gtttaatcca aaattatcaa acattgtgta tgcaagtggt
    11101 aacactaaaa ttgtcgcaac tattaaacgg actttttcat atttccgacg tcttttttca
    11161 tcgtaatcaa ttggcgcagc agggaaccca atcgctttta actcttttaa tatgatggtg
    11221 aaagaatctt tatcactata tgtaaaggtc acttttttct gattgacatt tacttttgct
    11281 tcgatgttta atgttttaaa gtgctcagta attgccatgg cacaattcgg acaaccaata
    11341 tttgggatta ttaatgttgt tttcatattt ccttaccagt tagccgttgc taacactata
    11401 attataatag tataataaaa ctaattcaag taatactaat tgttgttagg atagtttact
    11461 tgaatgaaat aaataatgat gaacggaggt gaaacataaa cattgcaata aatgataaaa
    11521 ctaaggatgt tagggaatat aaaatccttt tttactgaaa taaaaattaa taaataagca
    11581 tataaaacaa tgatatgtta tttttaaata aaaataggat agtaaaacag tttatttttt
    11641 aggtgtttta aggtatcttt tatatgcata caacggaaag agagtttgta aagatggtat
    11701 taatcgaaat taaagatcta catgcatccg taaatgatat tgaaatatta cgtggtgtta
    11761 acttaaaaat taaccgtggc gaagtagtgg ccttacttgg tcctaatggt catggcaaat
    11821 caaccttact acaagtaatt atgggtcacc ctaattataa agttacgaaa ggtgaaattt
    11881 tatataaagg tcaagatgtt ttagcaatga gtgttgacga aagagctaga gcagggcttt
    11941 ttcttgcaat gcaaaaccca agtgaggttc ctggtgtaca gattagtgat ttcttacgcg
    12001 ccgctatcaa tacaaaacgt gaaaaaccaa ttaaaccaat tgaacttttc cgcgtgattg
    12061 ataaagcaag caaagaaatg caaatgcccc acgaccttgc ctcgcgtaat ttaaatgaag
    12121 gtttttctgg cggagagaag aagcgtcatg aaattttgca aatgaaatta ttaaaacctg
    12181 aatttgcaat gttagacgaa attgattcgg gtcttgatgt tgacgggatt cgaattgtct
    12241 cgcaacaaat aaatgaagag aaaaaagata aaacatttct cgttattagt cactacgcaa
    12301 gactatatga acttattaat ccaacgcgcg ccattgtaat gattaatggt cgagtagcga
    12361 ttgaaggcgg tatggatgtc attaagcgca ttgaccaaga aggttatgaa tggattcaag
    12421 tagaacacaa cattaatatt gaacgcgaag acaaacgccc aattgtatta gaaatatgtg
    12481 cggtcaatga aagaagtaaa caatgagtgt ttttaaagcg ttattaataa ataaaatgaa
    12541 tattaatgat gacacgctca tcttaagtga aaacgatact gtttatcata tttttgacga
    12601 aaacgactta cctacttctg tgactaaagt tatattaaaa tcatcaaaag acattgattt
    12661 tgtcgttgta ttacggcaaa acaaaaagtt cattacctat gaacttgcgc catatacgcg
    12721 cggaaaagta atttttatgt gtagtgagga aaatcttagc attgatcgcg aaattatttt
    12781 acttgaaggt gctgaagtta aattaattat gcctgatttc cataatggta atcgcaaggt
    12841 aaatattgaa acgaaactat cagaaagaaa agctagagca gaatggcatc ttgctactta
    12901 ttcacaaaat attgataaga aagtatttaa tatctcgttt tcacattttg gaaatgagtc
    12961 atttgctg
//
LOCUS       DAOPCD010000006        12489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_5010_length_12489_cov_6.678704, whole genome shotgun sequence.
ACCESSION   DAOPCD010000006 DAOPCD010000000
VERSION     DAOPCD010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 12489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12489
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5010_length_12489_cov_6.678704"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..424
                     /locus_tag="PLQ55_00470"
     CDS             95..424
                     /locus_tag="PLQ55_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28448.1"
                     /translation="MLRFWLYVFAYWYTAAIVLVLFLAFALIGLTIFERYPFYHDLLA
                     ERSKGEEKRSLTIAFLVFIMAINIFWRIYGEAYKYIILAGVLGWGIGDALAALDGKQK
                     ISIVIET"
     gene            425..667
                     /locus_tag="PLQ55_00475"
     CDS             425..667
                     /locus_tag="PLQ55_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28449.1"
                     /translation="MLSTKKTIQGIFAMFLGSTIVIFPVLFLVGSKDWIYSLITALVA
                     GIIGTFVELYTKDGWDTLTLPSALMVILYVASIIVQ"
     gene            801..3533
                     /locus_tag="PLQ55_00480"
     CDS             801..3533
                     /locus_tag="PLQ55_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28450.1"
                     /translation="MNNIISEHITVEKPTLVKFWQKYKAKIFSASVTTLIVVLLFLFS
                     GNAYVEFAISKRLEALLLLSLLGISGILFLLNISFVKVKNYVLSLKSNLKNKKIILTL
                     VFSAVFIGLAAATLIINIVSNRGNFTGILRMISVYATIVMLVVTYKKERIIDTFLLSV
                     RILIIASLAVFILSLFLPSHYVTEIYVTKANTLVYNYFHLSFILRSWGYISAGIPRFS
                     SIFWEPSIAAGILSIALFVRIFYKKHSMFDTFLYVIAVVFTLSTGGIFLFGLLFIYYI
                     MTVLDKREKIVFGSIILVGSIFAFIFRESIFSALATLSPQLFGKLSTQSTPFLTRLNS
                     LPTFIKIFGEKPIFGHGPFGAITRYYEVIAGSSLDAATSTFGFLIAAFGSMGVFLVAL
                     IITAPLFLQEKWTNRIFASLFFIVLLNLQNVPFLALFMLFPVIFILEGVFNKKTSLTE
                     NNPVNERVYAYFFMSKTEGSAVVKNINGALIIKGLSMIVGIIAIPVYRTFFISEEYYG
                     LWMAIISILTWILFFDFGFGIGLRAKLGPLLDAGEEKDAQEKISSTYVGSIFIALGLL
                     VVFAVLIWSLDLNGILNTPTSLVSEMQLKISMSILAAGFCIEFVLKNVLFVLYSMRKS
                     AIASFVTFLSMLSLLLFFIIFKDFNITNRLLVSSIVYVVCINVPLIVASFVLYARKKA
                     LLPTFKGFSLASMKSVMSIGLVFFLIQIGFLFITQTDGLLITSMFSTNANAQYTYYSR
                     IFVFFVGLMGAVVQQPIWSAISAAREQGDTKMINKYIKVTFGIALFLFVICLFIGVGL
                     PFIFELWLGDHAPTVNSFFVIVFLLYSFIFLFSNALIIVANGLTILKSQAVITLIVAA
                     LKIPVLIGLMYLLPQLEWSVVVLVTTLCYVPYLIFLPIYIHKMIKKVRHEEKIA"
     gene            3514..4623
                     /locus_tag="PLQ55_00485"
     CDS             3514..4623
                     /locus_tag="PLQ55_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28451.1"
                     /translation="MKKKSLKYILFMLSALIAPALLSVGVALADVNFNYFVMSNAAKY
                     IKEGEEKMLVRLDNKDEINEDWRSFATTYNRRHQTYSSIFRHYSDVVVSTAGSERNVH
                     LADINLRSSNVVDTIEGIKIIDMGHSFQDLLTADHSLVISTLYATTLMNEKGVSEMKD
                     LLNVEVNVTRDNLTIVHKVRAIYDVNVKDEYANNKYYETFGDLFFVIRENTPLFDNPE
                     YYLTLGRDQDHNITIYDDLQYLKTKWAANVAKTSIDSEAQTLINKYDELIKDASFNLL
                     RASFIIVGALVTIFNIVVITLKVKQGKKDWALKKFDGWTIALLIVMGAPLLLMLVTSN
                     IPLFGLILNRLTPLGIALYVLSALSVYLAYKALCT"
     gene            4663..5871
                     /locus_tag="PLQ55_00490"
     CDS             4663..5871
                     /locus_tag="PLQ55_00490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK28452.1"
                     /translation="MKKTCVIVSTMYCPTGSASGLRISSVVDVLITLGYDCHVIGLSD
                     YDVFKTHEVKPGYTVHSIYNIKRKNNAFEKLKVFLYPKKRFKTIIEKIKQERKIDLLI
                     IYQQLPLQSSRYILNVAKKEKIKLLYDFVEWQVLGQQNLVSYFTFYRPNQKYIKKYSK
                     YGLNIAISTFLNEYFLSQNIDSLYVPFFFDVKSKVYVEDNKPFKPNQDKIYLGYFGKP
                     FGGRDTIINAIKGILLLPKEKQAKFEFIMAGPNDNDLIKLGLTPAELEAAQPFLKVLG
                     RIPHEQLVDIYKYIDYTILLKPAEKRFSKAGFPTKVSESLSYAVPSICNLTGDLELFL
                     EHEKNAVVLASDSPEDFAKGMEEVLAIDEKQYLVMRKEARVSAETKLDISVYSEKIKE
                     LLTKKSPTEE"
     gene            5882..7051
                     /locus_tag="PLQ55_00495"
     CDS             5882..7051
                     /locus_tag="PLQ55_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013858318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPK28453.1"
                     /translation="MKKIVVIGLGYVGLANAVLLSLNDEVVAVEINEQRIDLVNKRIA
                     PIQDAEIEHYFANHKLNLVATSDLAWALKDAEFVVIATPTDYDTNKNFFDTSSVDSSI
                     EKIHKINKNVIVIIKSTIPVGYSENIQKKYSDMEIIFSPEFLREGKALYDNLYPSRIV
                     IGSHSPQAHAFAELLKKNTLKEDVQVLFTGPTEAEAIKLFSNTYLALRVAFFNELDTY
                     AEIKGIDTKQVILGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYKGVPQNIIGA
                     IVDANRTRKDHVADMIVAKNPKVVGVYRLVMKNNSDNFRSSSIQGVMKRISEKGIKVV
                     VYEPALAAKDFFGSEVIRDFDEFVKMSDLIVANRHYKELAEVSDKVYTRDIFNRD"
     gene            7069..8103
                     /locus_tag="PLQ55_00500"
     CDS             7069..8103
                     /locus_tag="PLQ55_00500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK28454.1"
                     /translation="MAKNNIGVIGFYKKGEQFNSGQIVKTVSVVDALKELPDTKVYVM
                     NTYKWKYRGIFLFVKIFFSFFKCKNIILITAQKGIRVFTPLLSFFNKIFRWRIHYIVI
                     GGWIGKKVAKQKALKRIGKFYQVYVETTKIQEELASLGITNTTILNNFKDLPFASPED
                     LKKEHAVPYNICYFSRVEPEKGVEDIINVLANINKDKLLYKLTIYGVIRPNYKEDFLA
                     FVEKHKDYVEYAGVIKYDQSVNTIKDYFLQIFPTQYPTEGIPGSIIDSYYAGVPVVAA
                     RWNSFHEIINEGETGLGYSLGNLEELEKTLLKVIAEPNIITNMKGNCQQKAHEYSKEH
                     FLATFPQHFL"
     gene            8116..9030
                     /locus_tag="PLQ55_00505"
     CDS             8116..9030
                     /locus_tag="PLQ55_00505"
                     /inference="COORDINATES: protein motif:HMM:NF018378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HPK28455.1"
                     /translation="MKLRNFTFRNIRKWFRMVTGRSILHVNQGPGKHYSLTEVKGYYN
                     DLTEKITKDKRAAENYIPKQKNAKNDEFAFSIEIFQYGLAAYDLFLETNEEIYKEKFL
                     LMVEWAMNNQKDNGGWNTFVERHPEHPYSAMAQGEGISLLVRAYVLTQDDLYLAAAEK
                     AFNFMKLPVEKGGTTLYTDDLVLLQEYTHLPTVLNGWIFALFGVFDYYLMTKDEDVNK
                     FFDYNVRSIASALPQFDNGYWSKYDIDQKIASPFYHQVHIAQLRVLSELTNNKTFTEF
                     ADKWEGYASKRKNRRKAFWRKARQKLSE"
     gene            9079..9840
                     /locus_tag="PLQ55_00510"
     CDS             9079..9840
                     /locus_tag="PLQ55_00510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK28456.1"
                     /translation="MNTTTPPLVSIITPLYNCEDIIRETIGSVLAQTYPHWELIIVDD
                     VSTDNSREVVKEYVAKDKRIKLIELSENGGAAIARNKGIEVAEGRFIAFLDSDDLWKE
                     TKLEKQVNFMLENDYAFTCTDYEQLVDDTKKIKLIIKARVKADYRIVIRYNPIGNSTV
                     MYDTLKMGKVHIPEVRKRNDYALWLKILRMEKYVYGINEVLATYRIRKKSLSRNKVKL
                     IKHQWYVYRKLEKINFFKSLYILWFVIITKIFRIK"
     gene            complement(9911..11086)
                     /locus_tag="PLQ55_00515"
     CDS             complement(9911..11086)
                     /locus_tag="PLQ55_00515"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HPK28457.1"
                     /translation="MYNKEEIVNIGKQAYDKVDSVANLAKKYGVAVASIYNWKRLYAQ
                     KQGLVKLHEETNYETMNEAQLKLELMKKDIEIARLKKRLRGERGWGKEGIRYYTRLEY
                     QIVAELSYKYEVKKLCRAMNVSRSGFYIWQERVNRPSEKVLQRARDIQLFRKYHSRHQ
                     SHGYRWLNHKIRLDLGIFMSDQYAHKCCKIAGIKSKARHNRYKRPGNEGKTYPNLILS
                     NMNINRPLEVIVSDMTAMYIKGKYHELTLYMDLYNNELIGHSLSDKRGDPNTYHDGLK
                     QVLNKKEEQRNLDMILHSDQGSVYSSKSYNELLPNYNIKRTMSRAGTPTDNGAMEAIN
                     GWLKEELFLDFKINEADNIEKAINDYIHYFNYERPACALNYLTPIQYKVKYLLNSNQ"
     gene            complement(11261..12094)
                     /locus_tag="PLQ55_00520"
     CDS             complement(11261..12094)
                     /locus_tag="PLQ55_00520"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HPK28458.1"
                     /translation="MIVFRKKDKIKQLDMLNFKLERELQNFIEENLKEIFQFEFLATE
                     YPINNFRFDTLAYDRENNTFTIIEYKRGKNESLVDQGLAYLSTLLDRRSDIVLLFNKV
                     FEANKQIEDFEWDLVRVYFVSTQFTEFQRAATSFKDMPFLLFEVKRFEGDLYVISEVL
                     TKSKNTTPKVGNGPVVPADIKVYTEDDHKQNIPARVVELYDEVRDRLLELDDVEIKPL
                     KIYIAFKTLNKNFCDVKIQKDSLLVYLNLPHGRLDDAYNKTRDVSKIGHHGNGDYTFK
                     L"
BASE COUNT         3788 a         2248 c         2299 g         4154 t
ORIGIN      
        1 agcgatgaat gatatctttt attgttatac caccaaacat aactgaatac ttcttattct
       61 aattccttta tatttttaaa tacttttcct cctaatgctt cgattttggc tttacgtctt
      121 tgcctattgg tatacggccg ctatcgtcct cgtactattt cttgcttttg cccttattgg
      181 gcttacgatt tttgaaagat atcctttcta ccatgactta ctagcagaac gcagtaaagg
      241 cgaagaaaaa agaagcctta ccattgcctt tctcgtcttt attatggcaa taaatatatt
      301 ttggaggata tacggtgaag cctataaata tattatcctt gccggcgttt tagggtgggg
      361 catcggtgat gcgcttgcgg ctttagatgg aaaacaaaaa attagtattg ttattgaaac
      421 ataaatgctt tctaccaaaa agacaattca gggcattttt gcaatgtttt taggttcaac
      481 catcgtcatt ttccctgtcc ttttccttgt tggcagcaaa gattggatat atagtttgat
      541 tactgctctt gtcgccggaa ttatcggcac ttttgtcgag ttatacacaa aagacgggtg
      601 ggatacactt actttgccat cagcgttaat ggttattctt tatgtcgcaa gtattatagt
      661 tcaatagtca accataacat ttaaacacag aggaaatatg tacaaaaaca gggcaaaatt
      721 aacctctttt tcatttaaag gttattattt cttaatataa taatagacaa agatgcggaa
      781 catgatataa tatgttatct atgaataaca taattagcga gcatatcaca gtagaaaaac
      841 caacacttgt aaagttttgg cagaagtata aagcaaagat tttttcagca tcagtaacga
      901 cgcttattgt cgttttactt tttttattta gcggtaatgc ttatgtcgaa tttgctatat
      961 cgaagcggct agaagcctta ttacttcttt cactattagg catttccggc attttattct
     1021 tgttaaacat tagttttgtc aaggttaaaa actatgtttt aagccttaag agcaatctaa
     1081 aaaacaaaaa gataattctg acgcttgttt ttagtgcggt ctttattggg ttagcagcgg
     1141 ctactttaat tattaacatt gtgtcgaatc gtggcaattt taccggtatt ttgcgaatga
     1201 tttcggtata cgcaacgatt gtgatgctgg tcgttactta taagaaagag cgcataatcg
     1261 atacatttct attatcggtg cgcattttaa ttattgcttc cttagccgta tttattcttt
     1321 cgcttttttt accatcacac tatgttaccg aaatttatgt tacgaaggct aatacgctcg
     1381 tctataacta tttccaccta agttttattt tgcgcagttg gggttacatt agcgcaggta
     1441 ttccgcggtt tagttccata ttctgggaac ctagtatcgc cgctggtata ttgtcaattg
     1501 ccttatttgt gcgtatcttt tacaagaagc atagtatgtt tgatactttt ttatatgtga
     1561 tagccgttgt atttacatta tcgaccggcg gtatttttct ttttggcctt ctctttattt
     1621 attacattat gacggtatta gataagcggg agaagattgt ttttggctca attattttag
     1681 taggatcaat ttttgccttt atctttaggg aatcaatctt ttcggcttta gcgacattat
     1741 ctccgcagtt atttgggaag ttatcaacgc agagtacacc gtttttaacg cgcttaaatt
     1801 cattaccaac ctttattaaa atttttggtg aaaagcctat ctttggtcac ggaccttttg
     1861 gcgcgattac tcgttactat gaagttattg ccggttcgtc ccttgatgct gcaacttcaa
     1921 cctttggctt cttaattgcg gcctttggtt ctatgggtgt ctttctcgtc gcgcttatta
     1981 ttaccgcgcc attattttta caggagaaat ggactaaccg tattttcgct tcgctctttt
     2041 tcatcgtttt acttaactta caaaacgtcc ccttcctcgc tctctttatg cttttcccgg
     2101 tcatctttat tcttgaaggt gtctttaata aaaagacttc gttaaccgag aataatcccg
     2161 ttaatgaaag agtgtacgct tatttcttta tgtcgaagac ggaaggttca gcggtcgtta
     2221 aaaatattaa tggtgcttta attatcaaag gcttatcaat gatcgtaggt attattgcta
     2281 tcccagtata tcgaacgttt tttattagtg aagaatatta tggcctttgg atggcgatta
     2341 tctctattct tacgtggatc ttattctttg actttggttt tgggattggc ctacgggcta
     2401 agttaggacc actcttagat gcgggcgaag aaaaagatgc gcaagaaaaa atatcatcaa
     2461 cctatgttgg ctcaatcttt attgccttag gattattagt cgtctttgcg gttttaatat
     2521 ggtcacttga tttaaatggc atcttaaata caccgacaag tcttgtttct gaaatgcaat
     2581 taaaaatatc gatgtctatt ttagcggccg gcttttgtat tgagtttgtc cttaaaaatg
     2641 ttttgtttgt gctctactca atgcgtaaaa gtgcgattgc atcgtttgtc acgttcctaa
     2701 gcatgctaag tcttttactt ttctttatca ttttcaaaga ctttaatatc actaatcgct
     2761 tactcgtttc gtcaattgtc tatgtcgttt gtattaatgt cccccttatt gtggcttcat
     2821 ttgtcttata cgcccggaaa aaagccttat taccaacgtt taaaggattt agcctagcgt
     2881 caatgaaaag tgtcatgtcg attggtctcg tctttttcct tattcaaatt ggtttcttgt
     2941 ttattaccca aactgatggt cttttaatta caagcatgtt ctctactaac gcgaacgcgc
     3001 aatatactta ttattcgcga atattcgttt tctttgttgg ccttatgggc gcggtcgttc
     3061 aacaaccgat ttggtccgcg atttcggcgg ctcgggaaca aggcgacacg aaaatgataa
     3121 ataaatatat taaagtgaca tttggcattg ccttattcct ttttgttatt tgtctattca
     3181 ttggcgtggg cctgcccttt atctttgagt tatggcttgg tgatcatgcc ccgacggtta
     3241 atagtttctt tgttattgta tttttgcttt actcctttat tttcttattc tcaaatgcgc
     3301 ttattattgt tgctaacggg ctaacaatat taaagagcca agcagtcatc acgttaattg
     3361 tcgcggctct taaaatacct gttttaattg gtttaatgta tttattgccc cagcttgaat
     3421 ggagcgtggt cgtccttgtt acgacgcttt gctatgttcc ttatcttatt ttcctcccca
     3481 tatatattca taaaatgatt aagaaggtgc gtcatgaaga aaaaatcgct taaatacatt
     3541 ttgtttatgc ttagtgccct aatcgcgcct gcgcttttat cggtcggtgt tgcccttgct
     3601 gacgttaatt ttaattattt tgtgatgagc aatgcggcaa agtatattaa agaaggcgaa
     3661 gaaaagatgc ttgttcgcct tgataataaa gacgaaatta atgaagattg gcgcagtttt
     3721 gccacaacat acaaccgccg ccatcaaacg tattcatcga tctttagaca ttatagcgat
     3781 gttgttgttt caactgctgg tagtgagcga aatgttcatc ttgctgatat taatttgcgg
     3841 agctcaaatg tggttgatac tattgaaggc atcaaaatta ttgatatggg tcatagtttc
     3901 caagacttac ttaccgctga tcattcactc gttatttcaa cattgtacgc cacgacctta
     3961 atgaatgaaa aaggcgtaag tgaaatgaaa gatctcctta atgttgaagt taacgtaacc
     4021 cgtgataatt taacgattgt tcataaagtg cgggcgatat acgatgtgaa tgttaaagat
     4081 gaatacgcta ataataagta ttatgaaaca tttggtgatt tattctttgt tattcgggag
     4141 aatactccac tttttgataa tcctgagtat tacttaaccc ttggtcgcga tcaagaccat
     4201 aacattacca tttatgatga cttacaatat ttaaaaacta agtgggcggc taatgtcgct
     4261 aaaacatcaa ttgatagcga ggcgcaaacc ctcattaata agtatgatga gcttattaaa
     4321 gatgcttcat ttaatttact tagggcttcc tttattattg tgggcgcact agtgaccatc
     4381 tttaatattg tcgtaattac tttaaaagta aaacaaggga aaaaagactg ggcgttaaag
     4441 aaatttgatg gttggaccat tgccctatta atcgttatgg gggccccgct tttactaatg
     4501 ctcgtgacaa gtaacatacc cttatttgga ttaatactta atcgtcttac cccgttaggt
     4561 atcgcgcttt acgtactttc agccttaagt gtttacttag cctacaaagc actatgtacg
     4621 tagcaagaag cggaagtgag caaggaaaag gagaactaag atatgaaaaa aacatgtgta
     4681 attgtatcga caatgtattg tccaaccgga tcggcaagcg ggttacgtat ttcttcagtg
     4741 gtggatgttt taatcactct aggatatgat tgccatgtta ttggtttatc tgactatgac
     4801 gtctttaaga cccacgaagt aaaacccggt tacaccgtgc attcaattta caatattaaa
     4861 cggaaaaata atgcttttga aaaattaaag gtgtttttgt atcccaaaaa acgctttaaa
     4921 accattattg aaaagattaa acaggaacgg aagattgacc ttttaattat atatcaacaa
     4981 ttaccgctgc aatcatcacg ttatatttta aatgtggcga agaaagaaaa aataaaacta
     5041 ttatacgatt ttgtcgagtg gcaagtttta ggccaacaaa atttagtttc ctattttact
     5101 ttctatcgtc ctaatcaaaa atatattaaa aagtattcaa agtacggctt aaatattgcc
     5161 atctccacct tcttaaatga gtactttcta tcacaaaaca ttgattcact gtatgttccg
     5221 tttttctttg atgttaaaag taaagtctat gtcgaagata ataaaccatt taaacctaat
     5281 caagacaaga tatatcttgg ctactttggt aaaccttttg gggggcgcga cacaattatt
     5341 aatgcgatta aaggcatctt gttattgcca aaggaaaaac aagcaaaatt tgaattcatt
     5401 atggcgggtc caaatgataa cgatttaatt aaactaggat taacgccagc tgaacttgaa
     5461 gccgcgcaac catttcttaa agtcttaggc cgcattcccc atgagcaatt agttgatata
     5521 tataaatata tagactatac tattttgctc aagcctgccg aaaaaagatt ttccaaggct
     5581 gggtttccga ccaaggtttc ggaaagctta tcgtatgcgg tgccgtctat ttgtaactta
     5641 accggtgact tagaattatt cttagagcac gaaaaaaacg cggttgtcct tgcttctgat
     5701 tcccccgaag actttgctaa agggatggaa gaagtattag cgatagatga aaagcagtat
     5761 ttagtgatgc gtaaagaggc tcgcgtcagt gcggaaacaa aactagacat cagtgtctat
     5821 agtgaaaaaa taaaagaatt actcacaaag aaatccccaa cggaagagta aggaggagaa
     5881 aatgaaaaaa atagtagtga ttggtcttgg ctatgttggc cttgcaaatg cggtcttact
     5941 ttcattaaac gatgaagttg ttgccgtgga aattaatgaa caaagaattg acttagtaaa
     6001 taagcgtatt gctccgattc aagatgcgga aatcgaacat tattttgcaa accataagtt
     6061 aaatttagtg gcgacaagtg atcttgcctg ggcgctaaaa gatgccgagt ttgtcgttat
     6121 tgccacccca actgactatg acacgaacaa aaacttcttt gatacatctt cagtggatag
     6181 ttcgattgaa aaaatacata agattaataa gaacgtgatc gttattatta aatctacaat
     6241 tcctgttggt tactctgaaa acattcagaa gaaatatagt gatatggaaa tcattttctc
     6301 gcctgaattt ttacgggaag gtaaagcctt atacgataac ttatatcctt cacgaattgt
     6361 aattggcagt cactcaccac aggcgcatgc ttttgctgaa cttttaaaaa agaacacgct
     6421 taaagaagat gtgcaagtat tatttaccgg accaaccgaa gccgaagcga ttaaactatt
     6481 ctctaatact tacttagcgc tccgtgtcgc attctttaat gaactagata cgtatgcaga
     6541 aataaaaggt atcgatacga agcaagtaat tttaggtgtc ggccttgatc cccgcattgg
     6601 cagtcattac aataaccctt catttggcta tggcggctac tgcttaccaa aagacacgaa
     6661 acaattatta gcgaattata aaggcgttcc ccaaaacatt atcggcgcga ttgtcgacgc
     6721 aaaccggaca cgcaaagatc atgtcgcgga tatgattgtc gctaaaaatc ctaaagtagt
     6781 tggtgtctat cgcttagtaa tgaaaaataa ttctgataac ttccgttcat cctccattca
     6841 aggagttatg aaacggatta gtgaaaaagg tattaaggtt gttgtctatg aaccagcgct
     6901 tgcggccaaa gatttctttg gctccgaagt tattcgtgac tttgatgaat ttgtgaagat
     6961 gagtgactta attgtcgcta accgtcatta taaagaactt gccgaagtaa gcgataaagt
     7021 atatacccgc gacatcttta atcgcgatta ataaggaaag ataaagacat ggcaaaaaac
     7081 aatattggtg ttattggctt ctataaaaaa ggtgaacaat ttaatagtgg ccaaattgtc
     7141 aaaaccgtca gtgttgttga tgccttaaaa gaattaccgg acacgaaagt ttatgtgatg
     7201 aatacatata aatggaagta tcgcggtatt ttccttttcg ttaaaatatt tttctccttt
     7261 tttaaatgta aaaatattat tttaattact gcccaaaaag gcatccgtgt tttcacccca
     7321 ttattatcat ttttcaataa aatatttaga tggcgtatcc attacatcgt catcggggga
     7381 tggattggca aaaaagtcgc caaacaaaaa gcactaaagc ggattggtaa attttaccaa
     7441 gtatatgttg aaacgacgaa gattcaagaa gaacttgcaa gtcttggtat tacgaacaca
     7501 acaattctta ataactttaa agacttaccg tttgcgagtc cggaagactt aaagaaagaa
     7561 cacgctgtgc cttataatat ttgttatttc tcgcgggtcg aaccggaaaa aggtgttgaa
     7621 gatattatta atgttcttgc aaatattaat aaagacaaac ttttatataa attaacaata
     7681 tatggcgtta ttcgccctaa ttacaaggaa gactttttag cgtttgtcga aaagcataag
     7741 gactatgttg aatacgcagg tgttattaaa tatgatcaaa gcgttaacac tattaaagat
     7801 tacttcctac aaatcttccc cacacaatat ccaaccgaag gcattccggg ctcaattatt
     7861 gatagctatt acgctggggt tccggtggta gccgcgcggt ggaatagttt tcatgaaatt
     7921 attaatgaag gggaaaccgg acttggctac tccttaggta atctcgagga attagaaaag
     7981 acacttttaa aagtaatagc agaaccaaat attattacta acatgaaggg gaattgtcag
     8041 caaaaagccc atgaatattc aaaagaacac tttttagcaa cgtttcctca acatttttta
     8101 tgaggcaata aggatatgaa actacgaaat tttactttta gaaatattcg caaatggttt
     8161 aggatggtaa ccggccgtag tattttacac gtcaatcaag gacccggtaa acattattca
     8221 ctaaccgagg taaaaggtta ctataatgac ttaaccgaga aaatcacgaa agataaaaga
     8281 gccgcagaaa attacattcc taaacaaaaa aacgctaaaa atgatgaatt cgcctttagc
     8341 attgaaatct ttcaatatgg ccttgctgcc tacgatttat tcttagaaac aaacgaagaa
     8401 atatataaag agaagtttct tttaatggtg gagtgggcga tgaataatca aaaagataat
     8461 gggggatgga atacctttgt cgagcgtcac cccgaacacc cttattcagc catggctcaa
     8521 ggcgaaggca tatcgctttt agtaagagcc tacgttctca cccaagatga cttgtatcta
     8581 gcggcagccg aaaaagcctt taactttatg aaattaccag ttgaaaaagg gggtacaacg
     8641 ttatacaccg atgacttagt gttacttcaa gaatatacac acttaccaac tgtgcttaat
     8701 ggttggatat tcgccttatt tggcgtattt gattactatt taatgacaaa ggatgaagat
     8761 gtaaataaat tctttgatta taatgtgcgt tcaattgcaa gcgcccttcc ccaatttgat
     8821 aatggttatt ggtcaaaata tgatattgat caaaagattg caagtccttt ttatcatcaa
     8881 gttcatattg cgcaattacg agtattaagc gaactaacta ataataaaac ctttactgag
     8941 tttgccgata aatgggaagg ttatgcttca aaacggaaaa atcgtcgtaa agctttttgg
     9001 cgcaaagccc ggcagaagtt aagtgagtaa taccattatg aaaatgaaga tatataaaac
     9061 tttaagaggt aaaagactat gaataccacc acgccccctt tagtgtcaat tattactccg
     9121 ctttacaatt gtgaagatat tataagagaa acaattggtt ccgttttagc gcaaacttat
     9181 ccgcactggg aacttattat tgttgatgat gtttcgaccg ataactctag agaagtcgtt
     9241 aaagaatacg tagcgaaaga taaacgtatt aaacttattg aactaagtga aaatggtgga
     9301 gctgcgattg cccgtaataa aggtattgaa gttgccgaag ggcgctttat tgctttttta
     9361 gatagcgatg acttgtggaa agaaacgaag ttagaaaaac aagttaactt catgttagaa
     9421 aatgattatg cttttacatg cactgattac gaacaactag tcgatgacac aaagaaaata
     9481 aaactgatta ttaaagcaag agtgaaagct gattaccgca ttgttattag atataaccca
     9541 atcggaaact caacggtcat gtatgatact ttaaaaatgg gaaaagtaca tattcctgaa
     9601 gtgcgtaagc gtaatgatta tgctttatgg ctaaagatat taagaatgga aaaatatgta
     9661 tatggtatta acgaagtctt agcaacctac cgcattcgaa aaaaatcatt atcccgcaat
     9721 aaagtgaaac taataaaaca ccaatggtat gtatatcgta aacttgaaaa aataaatttc
     9781 tttaaatctt tatatatctt gtggtttgtt attattacta aaatatttag aattaaatag
     9841 ccaattgcgc ttaaataata gaatctttgc accagtcaag caaaagtaga cacgattttt
     9901 aaataattag tcactgattt gaatttagta ggtatttaac tttatattgg atgggtgtta
     9961 aataatttaa ggcacacgct ggtctctcat aattgaagta atggatgtaa tcattaattg
    10021 ctttttcaat attatctgct tcatttattt tgaaatccag gaataattct tctttcaacc
    10081 atccattgat tgcttccatc gccccgttgt cagttggagt tcccgctcga gacattgttc
    10141 gttttatatt ataattaggg agaagctcgt tgtagctttt tgaagagtat acggaccctt
    10201 gatcagagtg tagaatcatg tcgaggtttc gttgctcttc ttttttattg aggacttgtt
    10261 ttaggccatc gtgataggta tttgggtctc cgcgtttatc actaagacta tggcctatca
    10321 gttcattatt gtataaatcc atgtaaaggg ttagttcatg atatttcccc tttatataca
    10381 tcgctgtcat gtcacttaca atgacttcca gaggcctatt tatattcata ttactaagaa
    10441 tgaggtttgg gtatgttttc ccttcatttc ctggtcgttt atatcgatta tgcctggctt
    10501 ttgacttaat tcccgctatt ttacagcatt tatgcgcata ttgatcactc atgaagattc
    10561 ccaaatctaa tcggattttg tgattgagcc accgatatcc atggctttga tgcctactat
    10621 ggtacttacg gaataattga atatcccgcg ctcgctgaag tactttttcg ctcggacgat
    10681 ttactcgttc ttgccatata tagaatccac tgcgagaaac attcattgcc cgacataact
    10741 tcttaacttc atatttgtag gatagttcgg cgacaatttg atattctagt cgggtatagt
    10801 atcgtattcc ttctttcccc cacccccttt caccacgtaa cctttttttt aatcgggcaa
    10861 tctcaatatc ttttttcatt agttcaagtt ttaattgggc ctcattcatc gtttcatagt
    10921 ttgtttcttc atgcagcttt actaaccctt gtttttgagc atataaccgt ttccagttat
    10981 aaattgatgc cactgccacg ccatactttt tggccaggtt cgcgacacta tcaactttgt
    11041 cataggcttg tttgccaatg ttcactattt cttctttgtt gtacatgttt cctccggtac
    11101 atccatctta tcaaaactgg gtgcacccag ttttttatac cgtgtctaca tttagcttac
    11161 cgttcagttt ggtggtataa caataaaaga tatcattcat cgctgaatta caggacgcct
    11221 attgaatata aaagtgtctg tctcaataag tgttgacatc tcacagttta aaagtataat
    11281 ctccgttacc atggtgacca attttactta catcgcgagt tttattatac gcatcatcta
    11341 atctaccatg cggaaggtta agataaacaa gcaaactatc tttttgaatt ttcacatcac
    11401 aaaagttttt atttaaggtt ttgaacgcta tgtagatttt aagtggttta atttcaacat
    11461 catcaagttc aagtaaacga tctcgcacct catcatataa ctcgacgact cgggccggta
    11521 tgttttgttt atgatcgtcc tcggtataga ctttaatatc cgcggggaca acaggaccat
    11581 tcccgacttt tggagttgtg ttcttacttt ttgtaagtac ttcactaatc acgtagaggt
    11641 cgccttcaaa gcgtttgact tcaaataaga ggaacggcat atctttaaag ctcgtcgcgg
    11701 cacgttggaa ctccgtaaac tgagtggaaa caaaataaac ccggacgagg tcccattcaa
    11761 agtcctcaat ttgtttgttc gcttcaaaca ctttattaaa aaggaggaca atatccgagc
    11821 ggcggtctaa gagcgtgctg aggtaggcta aaccttgatc gacgagactt tcattttttc
    11881 cgcgtttata ttcaatgatc gtaaaggtgt tattttcccg gtcataggcg agcgtgtcaa
    11941 agcggaaatt attaataggg tattcggttg ctaaaaactc aaactgaaag atctctttta
    12001 ggttctcttc aataaagttt tgaagctctc gttcgagctt aaagttcagc atatctaatt
    12061 gttttatttt gtctttcttt ctaaaaacga tcatcgtgcg ccctcatttc tctaaatgta
    12121 tatattctta cttcgcaagt gaactaacac tttaataata gcaaatatgg gggagaaaaa
    12181 cccgattttt ggtatttcca cctatcaact gccaataatg caaccccccc ttcaaatcgt
    12241 gtattcttgc aaccccccct gtaaatttaa aaatggctaa attgctgatt tctaaagttt
    12301 gattgtaata tttcaatttt taacgccaaa ggggccaaaa aaagccagaa tttacgctta
    12361 tttaaagaaa aaatgccttg ataattgtat caaagcacta taacccttct ggtacaaccg
    12421 atggggctta aactttaaat gcaacccctc tccttgtaat cgttcaatcg tgcaacccca
    12481 ccccctaat
//
LOCUS       DAOPCD010000007        12001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_5276_length_12001_cov_4.312573, whole genome shotgun sequence.
ACCESSION   DAOPCD010000007 DAOPCD010000000
VERSION     DAOPCD010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 12001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12001
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5276_length_12001_cov_4.312573"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1761)
                     /locus_tag="PLQ55_00525"
     CDS             complement(67..1761)
                     /locus_tag="PLQ55_00525"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPK28459.1"
                     /translation="MQNIDALYEKWLKHPEMPKKLVSELKKMSAKAKTDAFYQNAEFG
                     TAGMRGILGAGTNRINIFTIRKAAYGFGLYVIKHVRNAKERGIVIAHDNRHMSEEFTE
                     ESMKTLRSLGIKTFVFDELKPTPLLSYAVRHLNAAGGIMITASHNPKNYNGFKVYDDT
                     GAQLTPEKVDLLIAELKTLPDYLDIKIDDKVNGYILLSDDVELTYYSKVMKIQLRRDL
                     PKKDFKIVFSPQHGTGRKLGMRALNVIGYNVIPVTEQMEPDPNFSGTKSPNPEEKAAY
                     SEAIKYARAYGAHLIITTDPDADRLGIVAKNKKGDFDYLTGNQSAALLLDYLCKTNIE
                     LGKDIKGKYLYTTIVSSSLANEVAESYGLKVKEFLTGFKFIGAQIAEDDSKNEDYFFF
                     GFEESYGLLAAPFVRDKDAIQAIVLYSEMALYYYLQGKTLGEVLDELYKKYGYHFDVT
                     KSVAFPGAAGQSEMQEILKRLRDNPPVTIGGLNILRYDDYLTSKSVTSGETTTLTLPK
                     SNVIRYHLEGGTTISIRPSGTEPKCKIYYGIITENEKEAQDLLTLLEADIKVRFTL"
     gene            complement(1765..2688)
                     /locus_tag="PLQ55_00530"
     CDS             complement(1765..2688)
                     /locus_tag="PLQ55_00530"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPK28460.1"
                     /translation="MEKLTFIISDIEANQRLDKYLKKIMPNAPQSFIYKLIRKKDIRV
                     NNKKSDAAYILNEDDTVVIFLTEAQVEEFIVPYTFTKIRRPLNIIYEDENILVVNKDT
                     GLLVHGTEFENELTLTNIVLTYLYDRGEFDPLRRGYIPSPVSRIDRDTSGVVIFSKKQ
                     SVHQVLANAFTQEDEVIRLYRLVVYGTLNKDEGAIELPLKRENGQTIPASDGQKAITH
                     YVVKKKGRTKTYVEAQLLTGRQHQIRAHFKAIGHPLVGDTKYGSSTEQGPLSLNAYKV
                     EFTALPAPLDYLNGHEFIADNTAYLLEMLGD"
     gene            2751..3545
                     /locus_tag="PLQ55_00535"
     CDS             2751..3545
                     /locus_tag="PLQ55_00535"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HPK28461.1"
                     /translation="MFKKEGFAEPAGLFSLSHLGLVLFFGTLAVLFYALTKKWTKAQV
                     MRFFKIYSLVLIALEIFKIIWNIVNYGTSAESLNRFIPLYYCSIFLYAFVIVAFTKGK
                     IQKAAYTWIMYGGLASGLAFLIYPSSSLLFYPFYHFLCLHSILFHMNFILIGFLIFRH
                     ELYTPNVKDFWYFVSFSLVFMSLAVILNKVLGTNLMFFEKPLAILPFETLNEWSPLFF
                     QSLIFILQLFGPFIGTHFVFYLFSRFTPFKIYKRNEENLTTNNDNI"
     gene            3574..3664
                     /locus_tag="PLQ55_00540"
     tRNA            3574..3664
                     /locus_tag="PLQ55_00540"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3608..3610,aa:Ser,seq:gga)
     gene            3839..5254
                     /locus_tag="PLQ55_00545"
     CDS             3839..5254
                     /locus_tag="PLQ55_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003336163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPK28462.1"
                     /translation="MDYDVIIVGAGPAGYFCAYELTEKRPNLKVLLIEKGRAINTRSC
                     PILKELIDKCPINKEGFQECYPSCSITCGFGGAGAYSDGKFNITSEFGGWMQDYLERE
                     ELLDLINYVDSINLKFGASNEITDPYTNKVKEIELRAMSVGVRLLRSKVRHLGTEENF
                     KILGRIFKHLQKKVTMLFETDVVEIITENNEIRGVVTKDNEQFTAPKVLLAVGREGSK
                     WLDTILEKRGIKMDQTQVDIGVRVECNDLIMEEINKHLYEGKFVYRTSTGNTVRTFCS
                     NPSGHVVMENYNGVINVNGHSYKDEAFRSENTNFALLVSHHFAEPFNKPNEYAVKVSS
                     LANQLACGSVLVQKFGDLLLGRRTTVKRLNEGFMRPTLKEAVPGDLALCLPHKTLQSI
                     IEMIRVLDKITPGIATEHTLLYGVEAKYYSARPEMTTEFETKAIKGLYLGGDGAGLTR
                     GLAQAGACGVYIARNMLKELK"
     gene            5256..6449
                     /locus_tag="PLQ55_00550"
     CDS             5256..6449
                     /locus_tag="PLQ55_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HPK28463.1"
                     /translation="MRKVGTSARGIRCPIIRSGDDLAQVVVNSVLEAAKAEGFTLRDR
                     DIIGITESLLARSQGNYASIDAIASEIKAKFPGGELGVIFPILSRNRFATLLKGIARG
                     AKKIYLMLSYPRDEVGNALLSEALIEKAGVNPYTDILTVPEYRKLFGYPKHEFTGIDY
                     VELYTDIVEQESCKIELIFANHAQMILNYTKHVLTCDVHTRQDSKHKLKVAGAETIYG
                     LDDLLTTPQLGEKGFNPQYGLLGSNKATDDSIKLFPRDGQPVVEKIQQMLLTKTGKHI
                     EVLIYGDGAFKDPQGHIWELADPVVSPFFTPGLSGTPSEVKLKYLADNDFKNLTGAEL
                     TKAIAASIKAKPKDLVGTKATLGTTPRQITDLVGSLCDLVSGSGDKGTPVVLIQGYFD
                     NYTDN"
     gene            complement(6450..6989)
                     /gene="hpt"
                     /locus_tag="PLQ55_00555"
     CDS             complement(6450..6989)
                     /gene="hpt"
                     /locus_tag="PLQ55_00555"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPK28464.1"
                     /translation="MMMKDLEKVLVTEAEITEIINRVSARIEKDYKGKDLVMIGILKG
                     GAPFMMDLIKKINLPLIIDFIRVESYFGGTTQGELVFRKDIEVDITNKHVLIVDDIID
                     SSRTLKMVIDLFKKRNASSIEVCTLLDKPSGRVIDYTPKYIGTTIGNEFVVGYGLDYD
                     EKYRNLPVIGVLKEEVYKK"
     gene            complement(6989..8470)
                     /locus_tag="PLQ55_00560"
     CDS             complement(6989..8470)
                     /locus_tag="PLQ55_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HPK28465.1"
                     /translation="MIAFDYEHYLTIQKREILKRIKLFDNKLYLEFGGKLIEDNHAAR
                     VLPGFHPSGKLEIFSSLKEQIEVIIVVNTNDIVANKIGSDNNISYQGEVERLIDEFRT
                     KDIAVCGVVFSFFSESIVTNAFIKKLARNNIKTYKHYVIEDYPHNLEKIFSEDGLGRN
                     EYVETTRPLVVVTAPGSGSGKLATCLSQLYHDNKNGIRAGYAKYETFPIWNLPLNHPL
                     NLAYEAATVDLGDFNVIDPYYLDKFNVKATNYNRDVDAFPVLKAIFEKMYGETPYESP
                     TMMGVNMVGFALGDEEAITAACKEEVIRRFYSAQKNLFLDKYTASSLDKIRMIMNKLG
                     IVETDRKCVLPALKKADETHVPCMALVLNDGKIITAKTSKLLSPAAALFLNSVKYLAN
                     INDEIPLISPNLIEPIEALKKEISAINDSVLNLNDTLISLAIQAPMNPLSDLALKQIE
                     KLKGAQAHSSVLLDKRDLQVLKQLGINVTEESVPASNKLYLRS"
     gene            complement(8486..9454)
                     /locus_tag="PLQ55_00565"
     CDS             complement(8486..9454)
                     /locus_tag="PLQ55_00565"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009360773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPK28466.1"
                     /translation="MSFSKDACIFSLTANKELTREVAKILGVNVSEAIVTHFADGETL
                     CEPTSSFRGKNCYVIQSTCKPVNERLMEILIFADALKRASAKSINVVMPYFGYARQDR
                     KNKPRQPITGRLVADLIHAAGIDRVVVIDLHAPQIQGFFTCLVDEMTAIPFLAKYFKD
                     KVDDDWVIVSPDHGGMARARRVAEFLNKPLAIIDKRRPKPNEATISHVIGDVKGKHCL
                     VIDDIIDTGGSSRAGAIALLEHGAKSVRIAATHGVFSPGAEVKLYEDNIFKEIVVTYS
                     IPTPEHMKGKPLTVLSLALMIAKAIEHINSGKSLTIVYEMFSNTEE"
     gene            complement(9460..10086)
                     /gene="upp"
                     /locus_tag="PLQ55_00570"
     CDS             complement(9460..10086)
                     /gene="upp"
                     /locus_tag="PLQ55_00570"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HPK28467.1"
                     /translation="MVFEIKHPLIDVKLSIMRDEKTKSKEFRESLDEIASLMSYEIFK
                     DIGTHASEDTFMTPTGVELQKIKLDNKIILVPILRAGVGMVDGIRNMIPTARIGHIGI
                     YRDEKTLEPHEYYFKLPKVDNPLVVLVDPMLATGGTAIASIDAIKARGYNNIRMATLV
                     ASPLGIERVSIAHPDVHIYVAAIDEKLNEHGYIIPGLGDAGDRIFGTR"
     gene            complement(10210..11076)
                     /locus_tag="PLQ55_00575"
     CDS             complement(10210..11076)
                     /locus_tag="PLQ55_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPK28468.1"
                     /translation="MRNKQLRILLAVTLTLNIILAVIKLGAGYLYNSPSLVGDGFNSF
                     FDIFISVMLLIVMKTASRAADENHHYGHQKYEGLIFFGLGLFILFTTVVLGINTVKDF
                     IAYFQGAEYVLPGTPALIVAIVSVFIKVFMFIINYRAYLKLKRPALKGDAINHLSDIF
                     ATAVSIISIVLAKFNFYIFEYIGTLIICVIIINSSINIIKEAAYYLTDASPSKKTVES
                     IWECILNVEGVICVDDLKVRRHMNNYYVDVEVGINKNLTLKEAHDISEKVHSQVEANF
                     KVLHCHVHINPH"
     gene            11190..>12001
                     /locus_tag="PLQ55_00580"
     CDS             11190..>12001
                     /locus_tag="PLQ55_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK28469.1"
                     /translation="MLELVNIKRYYTQKKGPTVKALDDVSLVFPETGMVFVLGKSGSG
                     KSTLLNVIGGLDQATSGDLIIKGKSTKKFKQNEFDSYRNTMVGFIFQEYNVLNDFTVG
                     QNIGIALELQGEKASNEKINAMLELLDMQGYGHRKPNTLSGGQKQRVAIARALVKNPQ
                     IIMADEPTGALDSVTGKQLFETLKKLSQDKLVIVVTHDHEFAENYGDRIIEFSDGKVI
                     RDVSKVKNEKQETNTVFSEEGITVSAGYELTAEDLVKINEYLRQNRDKTFIKV"
BASE COUNT         3818 a         2286 c         2057 g         3840 t
ORIGIN      
        1 cttacgcaaa tatgaaaaca actgaagata ataataatga tggtatcgta agcaccttac
       61 aaaaaattat aaagtaaagc gaactttaat atcagcttca agaagcgtaa gtaaatcttg
      121 cgcttctttt tcattttcag taataatacc gtaataaatt ttgcactttg gttcagtacc
      181 acttgggcga atcgaaatcg ttgtgccccc ttcaaggtga taacgaatga cattactctt
      241 tggtaatgtt aaagttgttg tttcgccact tgtcacactt ttactagtta aataatcatc
      301 gtagcgtaaa atatttaacc caccaatcgt gacgggcggg ttatcacgta atcgttttaa
      361 gatttcttgc atttcacttt gaccggcagc acctgggaaa gctactgact tagtaacatc
      421 aaagtgataa ccatattttt tgtaaagttc atctaaaact tcaccaagtg tcttgccttg
      481 taagtaataa tataaagcca tttcactata aaggacaatt gcttgtatcg catctttatc
      541 acggacaaat ggagcggcga gtaacccgta actttcttca aaaccaaaga agaaataatc
      601 ttcgttttta ctgtcatcct cggcaatttg tgccccaatg aacttaaagc cagttaaaaa
      661 ttctttaact tttaaaccat aactttccgc tacttcgtta gcaagactac tacttacaat
      721 tgtcgtgtat aagtattttc ctttaatatc tttgcctagt tcaatattcg ttttacataa
      781 gtaatctaaa agtagcgctg cactttgatt acctgttaaa taatcaaaat cgcctttctt
      841 atttttagct acaatcccta aacgatcagc gtcaggatca gtcgtaataa ttaaatgcgc
      901 accatatgct ctagcgtatt taattgcttc gctgtaggct gctttttctt cgggattagg
      961 actttttgtg ccggaaaaat tcgggtctgg ttccatttgt tcggtaacag gaataacatt
     1021 atagccaata acattaagcg cccgcattcc aagttttctc cccgtgccat gctgcggtga
     1081 aaagacaatt ttaaagtcct tcttcggtaa gtcgcggcgt aattgtattt tcattacttt
     1141 tgagtaataa gttaattcaa catcatcact taaaagtata tatccattaa ctttgtcatc
     1201 gatttttata tctaaatagt caggtagcgt ttttaattcg gcaattaata aatcaacttt
     1261 ttccggtgta agttgtgcac ctgtatcgtc ataaacttta aagccgttat agttttttgg
     1321 gttatgcgaa gcggtaatca ttattccgcc tgctgcattt aaatgccgca ctgcataact
     1381 tagtaatggt gtaggtttta attcatcaaa cacgaaggtt ttaattccta gactgcgcaa
     1441 tgttttcatt gattcttccg taaattcttc actcatatga cggttatcat gggcaataac
     1501 aatcccgcgt tctttagcgt ttctcacatg tttaatcaca tataaaccaa agccatatgc
     1561 cgctttacga atcgtaaaaa tattaattct atttgtaccc gcgcctaaaa ttccgcgcat
     1621 tcccgctgtg ccaaactcgg cattttgata aaaggcgtct gttttagctt tcgcgctcat
     1681 cttttttaac tcgctaacaa gttttttagg catttcaggg tgttttaacc atttttcata
     1741 cagtgcatca atgttttgca taatttaatc tcctaacatt tctaataaat aagccgtgtt
     1801 atcggcaata aattcatgac catttaaata atcaagtggg gctggtagtg cagtgaactc
     1861 taccttataa gcgtttaaag aaagtggtcc ttgttcggta ctactaccgt atttagtgtc
     1921 accaacaagc ggatgaccaa tcgccttaaa atgcgctctt atttggtgtt ggcgccccgt
     1981 taaaagctgc gcttccacat atgttttagt gcgtcccttt ttcttaacta cataatgagt
     2041 tatcgctttt tgaccatcac tagcaggaat cgtttgacca ttctcccttt ttaaagggag
     2101 ttcaatcgct ccttcatcct tgtttagtgt accataaacg actaaacgat ataaacgaat
     2161 aacttcatct tcttgcgtaa atgcatttgc cagcacttga tgaacgcttt gcttcttact
     2221 aaaaataaca acaccacttg tatcacgatc aatacggctg actggcgaag ggatataacc
     2281 gcgccgcagc gggtcaaatt caccacgatc ataaaggtaa gttaacacaa tattagttaa
     2341 ggttaattca ttttcaaatt cagtaccgtg cactaaaagt cctgtatctt tattaaccac
     2401 taaaatattt tcatcttcat aaattatgtt aagtggtcgt cttattttcg taaaagtata
     2461 aggcacaata aattcttcaa cttgcgcttc tgttagaaaa atgacgacgg tgtcgtcttc
     2521 atttaatatg taagcagcat cactcttctt attatttact ctaatatctt ttttacgaat
     2581 taatttataa ataaaactct gcggggcatt aggcataatc ttttttaaat acttatcaag
     2641 acgttgatta gcctcaatgt cactaatgat gaatgttagt ttctccatac tttaatttta
     2701 ccaaatatca gtgcgcatat ggtaaaatta gacaatgaaa acgagggcaa atgtttaaaa
     2761 aagaaggctt tgctgaacct gcgggactct ttagtcttag tcacctcgga ttggtgctct
     2821 tttttgggac gcttgcagtg cttttttatg cattaacgaa aaaatggaca aaagcgcaag
     2881 tgatgcgttt ctttaagata tatagtcttg tattaatagc gttagaaata tttaaaataa
     2941 tttggaatat cgtaaattat ggcactagtg ctgagagttt aaatcggttt atcccgcttt
     3001 attattgctc catttttctc tatgcttttg taattgttgc atttaccaaa ggtaaaattc
     3061 aaaaggcggc ttatacttgg ataatgtatg gcggacttgc ttcaggttta gcctttttaa
     3121 tatatccttc tagttcgctt ttgttttatc ctttctacca cttcttatgt ttgcattcta
     3181 tcttattcca tatgaatttt atcttaattg gttttttaat cttccgtcat gaactctaca
     3241 ctccaaatgt taaagacttt tggtactttg tgagcttttc actcgtattt atgtcactag
     3301 cggtaatctt aaataaagtg ttaggcacta atttaatgtt ctttgaaaaa cctttagcga
     3361 tccttccgtt tgaaacatta aacgaatggt caccactctt tttccaatcc ttaatcttta
     3421 ttctgcaatt atttggacct tttattggca cacactttgt cttttatctc tttagtcgtt
     3481 tcactccatt taaaatatat aaaaggaatg aagaaaactt aactacaaat aatgacaaca
     3541 tataattaaa tttgctataa taaccgcact gctggagaaa tacccaagag gctgaagggg
     3601 ctggcttgga aagctagtag gtctgtcaca ggacgcgagg gttcaaatcc ctctttctcc
     3661 gccagataag taaattagtc cgcatcatag tcggactttt ttataaatgt aataaagcgt
     3721 taccaatatt attaaattca actaacaata taagtttatt tattataata aaaagggaaa
     3781 ataaatttct agatacccct caaggcaaaa taatataaat taaggagacg cttttactat
     3841 ggattatgat gtgattattg tcggtgctgg accagcaggt tatttctgtg cttatgaatt
     3901 aactgaaaaa cgtccaaacc tcaaagtttt attaattgag aagggacgag ccattaatac
     3961 tcgctcatgt ccaattttaa aagaattaat tgataaatgt ccgattaata aagaaggctt
     4021 tcaagaatgt tatccgtctt gctcaatcac ttgtggcttt ggtggtgctg gagcctattc
     4081 tgatggcaaa tttaatatca ctagtgaatt tggtggttgg atgcaagatt atttagaaag
     4141 agaagaattg ctcgacctta tcaactatgt tgatagtatt aatttaaaat ttggcgctag
     4201 taatgaaatc actgatccct atacgaacaa agttaaagaa attgaactcc gtgcgatgag
     4261 tgtgggtgtt cgcttactcc gtagtaaagt aagacactta ggcactgaag aaaactttaa
     4321 aatattaggc cgtattttta aacatttaca aaagaaagtt acaatgttat ttgaaactga
     4381 tgttgtggaa ataatcactg aaaataatga gattcgtggt gtagttacaa aagacaacga
     4441 gcaatttact gcccctaaag tattactcgc ggttggacga gaaggtagta aatggctcga
     4501 cactatttta gaaaagcgcg gcatcaaaat ggatcaaacc caagttgata tcggtgtccg
     4561 cgtcgaatgt aatgacttaa ttatggaaga aatcaataaa catctttatg aaggtaaatt
     4621 tgtctatcgt acttctactg gtaatacagt ccgcaccttt tgttcaaatc caagtgggca
     4681 tgtcgttatg gaaaattata acggcgtcat aaatgttaat gggcactctt ataaagatga
     4741 ggcatttcga tcagaaaata caaactttgc actacttgtg tcacaccact ttgctgaacc
     4801 atttaataaa ccaaatgaat atgccgttaa agtatcttca ctggcaaatc aactcgcttg
     4861 tggttcagtc ttagtgcaaa agtttggtga cttattacta ggaagacgca ccacagtcaa
     4921 acgcttaaac gaaggcttta tgcggccaac attaaaagag gcagtgcctg gcgacttagc
     4981 gctttgttta ccacataaaa cactgcaatc aattattgaa atgattcgtg tgctcgataa
     5041 aattactcca ggtattgcga cagagcatac actactttac ggtgttgaag ccaagtatta
     5101 cagtgcccgt ccagaaatga ctactgaatt tgaaacaaaa gctattaaag gcttatattt
     5161 aggcggcgac ggtgctggac ttactcgcgg cttagcgcaa gctggagcat gtggcgttta
     5221 tattgctcgt aatatgttaa aggaattaaa gtaagatgcg gaaagtaggt acaagtgcac
     5281 gcggtattcg ttgtcccatt attcgcagtg gagatgactt ggcgcaagtt gtcgttaata
     5341 gtgtattaga agccgcaaaa gcggaaggat ttactcttcg tgatcgtgat attatcggta
     5401 tcactgagtc actccttgcg cgctcacaag gaaattatgc ttcaattgac gctattgcat
     5461 cggaaattaa agcaaagttc ccaggcggag aattaggagt tatcttcccg attctttctc
     5521 gtaatcgttt tgcaacatta cttaaaggga ttgcgcgtgg cgcaaagaaa atttatttaa
     5581 tgctttctta cccgcgcgat gaagtcggta atgcgcttct aagtgaagca ctcattgaaa
     5641 aagctggggt taatccttac actgatattt taacagtgcc agaatatcgt aaactcttcg
     5701 gctatccaaa acatgaattt accggcattg attatgttga actctataca gacattgtgg
     5761 agcaagaaag ctgcaaaatt gaattaattt ttgcaaatca cgcccaaatg attctaaatt
     5821 acacaaaaca tgtcttaacg tgtgatgttc atacccgtca agattcaaaa cataaattaa
     5881 aggtcgcggg agctgaaaca atttacggat tggatgattt attgactacg ccacaactag
     5941 gagaaaaagg atttaatccg caatatgggc tccttggttc aaataaagcc acagacgact
     6001 cgattaaact ttttccgcgt gacggacaac ctgttgtcga aaaaattcaa caaatgttac
     6061 taacaaaaac aggcaaacat attgaagttt taatttatgg cgatggtgct tttaaagatc
     6121 cacaaggaca tatttgggaa ctagcagatc ctgtagtgtc accatttttc actcctggat
     6181 taagcggcac acctagcgaa gtaaaactaa aatatctcgc tgataacgat tttaaaaatt
     6241 taacgggcgc tgaacttact aaagcaattg ccgcttcaat taaagctaaa ccaaaagacc
     6301 ttgtaggcac taaggcgacg ctaggtacta ccccacgtca aataactgac cttgttggat
     6361 cactttgtga tttagtaagt ggttctggtg ataaaggcac accagttgtg ttaattcaag
     6421 gttattttga taattacaca gataattaat tattttttat aaacttcttc tttcaataca
     6481 ccgataactg gcaagttacg atatttttca tcataatcta aaccgtaacc aacaacaaat
     6541 tcattaccga tagttgtccc aatatacttt ggcgtataat caattacgcg accacttggt
     6601 ttatcaagta gtgtacacac ttcaatcgaa ctagcgttac gctttttaaa gaggtcaatt
     6661 accattttta atgtgcggct gctatcaata atatcgtcaa caattaagac atgcttgtta
     6721 gtgatatcaa cttcaatatc cttacggaag actagttcgc cttgtgtcgt gccaccaaaa
     6781 taagattcaa cacgaataaa gtcgatgata agtggcaaat taattttctt aattaaatcc
     6841 atcataaacg gggcgccgcc ctttaaaatg ccaatcatca ctaaatcttt acccttataa
     6901 tccttttcga tccgtgctga aacacgatta ataatttccg taatttctgc ttcagtcact
     6961 aatacttttt ctaaatcctt catcataatc atgacctcaa atataattta tttgatgctg
     7021 ggacactctc ttcagtaaca ttaattccta gttgttttaa aacttgaaga tcgcgtttat
     7081 caaggagcac agaagagtgc gcttgtgctc cttttaattt ttcaatttgc ttaagcgcaa
     7141 gatccgataa cggattcatc ggcgcttgga ttgctagaga aatcaatgta tcgtttaaat
     7201 taagcactga atcgttaatc gcacttattt cttttttcag cgcttcaatt ggttcgataa
     7261 gatttgggga aataagcgga atttcatcat taatattcgc taaatattta actgaattta
     7321 agaataatgc agcggcaggg cttaaaagtt ttgatgtttt tgctgtaata attttgccgt
     7381 cattaagcac taaagccata cacgggacat gtgtttcatc ggccttttta agggcaggca
     7441 atacacattt acgatcagtt tcaacaatgc ctaatttatt cataatcatc cggattttat
     7501 caagactact agcggtatat ttatcaagga agaggttttt ttgcgctgaa taaaaacggc
     7561 gaataacttc ctctttacac gcggcggtaa tcgcttcttc atccccaagc gcaaagccaa
     7621 ccatattgac gcccatcatt gtcggtgatt catagggcgt ttcaccatac atcttctcaa
     7681 aaatagcttt taacacaggg aaagcgtcaa catcacgatt gtaatttgtc gctttaacat
     7741 taaatttatc taagtaataa ggatcaataa cattaaagtc acctaaatca acagtcgctg
     7801 cttcataggc taagttaagc gggtgattaa gcggtaaatt ccagattggg aatgtttcat
     7861 attttgcata tcccgctctt ataccgtttt tattatcgtg atagagttgc gataaacatg
     7921 tcgcgagttt cccacttcct gaaccaggtg cagtaacaac cacaagcgga cgcgttgttt
     7981 cgacatattc attacgacct aacccgtctt cactaaatat cttttctaag ttatgcggat
     8041 agtcttcaat cacgtaatgt ttatatgtct taatattatt tctcgctagt tttttgataa
     8101 aagcattcgt gacaattgat tcactaaaaa aggaaaagac aacaccacaa acggcgatat
     8161 ctttagtgcg aaattcatcg attaaccgtt caacttcacc ttgataagag atattattat
     8221 cactgccaat tttattagcg acaatgtcat tagtgttgac aacaataatt acttcaattt
     8281 gctcttttaa acttgagaaa atttcaagtt taccgcttgg atgaaaccca ggtaaaacac
     8341 gagcagcgtg attatcttca ataagtttgc caccaaattc gagataaagt ttattatcaa
     8401 acaatttaat ccgctttaaa atttcgcgtt tttgaatagt taaataatgt tcataatcaa
     8461 aagcaatcat aaactttaac tccttttatt cttcggtatt actaaacatt tcgtagacaa
     8521 tcgtgagtga tttgcctgaa ttaatatgtt ctattgcttt cgcaatcatc agcgcaagag
     8581 aaaggacggt tagcggtttt cccttcatat gttccggtgt tggaattgag taagtaacga
     8641 caatttcttt aaagatgtta tcttcatata atttaacttc cgctcctggt gaaaaaacac
     8701 catgcgttgc cgcaatgcga acagacttcg caccatgttc taaaagggcg attgcacctg
     8761 cgcgtgaact accacctgta tcgataatat catcaatcac tagacaatgt ttacctttaa
     8821 catcaccaat aacatgactt atagtcgctt catttggttt tggccgcctt ttatcaataa
     8881 tcgctagtgg tttatttaaa aactctgcca ctctgcgcgc ccgcgccatt ccgccatgat
     8941 caggagagac aattacccaa tcatcatcaa ctttatcttt aaagtacttt gctaaaaaag
     9001 gaatagcggt catttcatcg actaaacatg taaagaatcc ttgaatttgc ggggcgtgta
     9061 aatcaattac gacaacacga tcaattccag cagcgtgaat taaatccgcg acaagccgac
     9121 cagtaattgg ttgacgtggt ttatttttac ggtcttgtct agcgtagcca aaataaggca
     9181 tcacaacatt tattgatttg gctgaagctc tttttaaagc atcagcgaaa atgagaattt
     9241 ccatcaatcg ttcatttact ggtttacaag tagattgaat cacataacaa tttttcccgc
     9301 ggaatgaact tgtaggttca caaagtgttt ccccatctgc aaaatgtgtg acaatagctt
     9361 cagaaacatt tactcctaaa atctttgcta cttcacgcgt taattcttta ttggctgtta
     9421 atgaaaatat acaagcatct ttactaaagc tcataatctc tacctcgttc caaaaatccg
     9481 gtcaccagca tcaccaagtc cggggatgat gtaaccatgt tcatttaatt tttcatcaat
     9541 tgcggcgaca taaatatgaa catcggggtg cgctatgctc acgcgttcaa tcccaagcgg
     9601 gctcgccaca agtgttgcca tccgaatatt gttataacca cgagctttaa tcgcatcaat
     9661 cgaggctatt gcggtcccac cagttgctaa cattgggtca actaacacta ccagcggatt
     9721 atcgactttt ggtaatttaa aataatattc atgtggttct aatgtttttt catcacggta
     9781 tataccaata tgaccaattc tagcagtagg tatcatattt ctaatgccgt caaccattcc
     9841 aacccccgct cttaagattg gaactagaat aatcttatta tctaacttaa ttttttgtaa
     9901 ctctacgcct gtcggagtca taaatgtgtc ctctgaagcg tgggtaccaa tgtctttaaa
     9961 tatttcataa gacatcagcg aagcaatttc atcgagtgat tcacgaaatt ccttagattt
    10021 tgttttctca tcacgcatga ttgataactt tacatcaatc agcgggtgct taatttcaaa
    10081 aaccataagt ccctcctatt gaaatcatac atgtatatta tacttaatat aaatctaaaa
    10141 aagggtcttt aactattaaa aattttgtaa taagttaaaa accgaataat ctttacaaat
    10201 aatcgttatt tagtgggggt taatatggac atggcaatgt aaaactttaa aatttgcttc
    10261 cacttgacta tgaacctttt cactaatatc atgcgcttct tttaatgtca aatttttatt
    10321 aataccaact tccacatcaa cataatagtt attcatatgg cgtctaactt ttaaatcatc
    10381 aacacatatg acaccttcaa catttaaaat acactcccaa atgctctcta ccgtcttctt
    10441 acttggtgaa gcatcagtta agtaataggc tgcttcttta ataatattaa tacttgaatt
    10501 aataatgatc acacaaataa taagtgtccc aatatattca aagatataaa aattaaattt
    10561 agcaaggaca atcgaaataa tactgaccgc tgtcgcaaaa atatcagaaa gatgattaat
    10621 cgcgtccccc tttaaagccg gccgctttag ttttaaataa gcgcgataat taataataaa
    10681 catgaatact tttataaaga cactgacaat cgctacaatt aaggctggtg tgccaggtaa
    10741 aacatattcc gcaccttgaa agtaagcaat aaagtcttta acggtattaa ttcctaacac
    10801 tactgtcgta aataaaataa atagacctaa accaaagaag ataagtcctt catatttttg
    10861 atgaccataa tggtgatttt catcagcagc gcgactcgca gtcttcatca caataagtag
    10921 catcacacta ataaaaatat caaaaaagga attaaagcca tcaccaacaa gcgaaggtga
    10981 attatataaa tatcccgcac cgagcttaat caccgcaagg ataatattta aggtcaatgt
    11041 gaccgctaaa aggatgcgta gttgtttgtt tctcatattt tgtattatag tacttttcaa
    11101 atgctaatta aattgattaa tcataaaact actatatttc tatataatgc tataatttat
    11161 tatagaaatt agtgataagg aattttatta tgctagaact tgttaatata aaaagatatt
    11221 atacgcagaa gaaaggccca actgttaagg cgcttgatga tgtttcactc gtatttccag
    11281 aaacagggat ggtgtttgta ttaggtaaat caggttcagg aaaatcaact ttacttaatg
    11341 tcatcggcgg acttgaccaa gcgacaagtg gggacttaat cattaaaggt aaatctacca
    11401 aaaaatttaa acaaaatgaa tttgatagct accgcaatac aatggtcggt ttcatcttcc
    11461 aagaatacaa cgttttaaat gacttcactg tcggtcaaaa tataggcatt gcgcttgaac
    11521 tacaaggtga aaaagcatca aacgaaaaaa ttaatgcaat gcttgaatta cttgatatgc
    11581 agggttacgg gcaccgcaaa ccaaatacac tttccggcgg acaaaagcaa cgagtagcga
    11641 tcgcccgcgc tcttgttaaa aacccgcaaa ttattatggc cgatgagcca accggtgcac
    11701 ttgatagcgt cactggtaaa caactttttg aaacactaaa aaaattatca caagataaac
    11761 ttgtcatcgt tgtgactcat gaccacgaat ttgccgaaaa ctatggtgac cgcattattg
    11821 aatttagtga cgggaaagta attcgtgatg tttctaaagt aaaaaacgaa aagcaagaaa
    11881 caaatacagt ctttagcgaa gaaggaatta ctgttagtgc gggttatgaa ttaacagcgg
    11941 aagatttagt caaaatcaat gaatatttac ggcaaaaccg cgataaaaca ttcattaagg
    12001 t
//
LOCUS       DAOPCD010000008        10471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_6178_length_10471_cov_4.471486, whole genome shotgun sequence.
ACCESSION   DAOPCD010000008 DAOPCD010000000
VERSION     DAOPCD010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 10471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10471
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6178_length_10471_cov_4.471486"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLQ55_00585"
     CDS             <1..794
                     /locus_tag="PLQ55_00585"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPK28470.1"
                     /translation="YIINAVEKFPKALYTFYVDENLETGEIERGYADILVEGHEYNTA
                     EDYYVVILGKGREDSLFTFDAINSEAPWEVPFKEGKQEAVKQFYTDEIEKAHQHLDYH
                     EVVVPLIIKAERLIFYAMAGSYLTSALILIVIIPNFRKDKVTFGKVITGTIVLNRLGY
                     KMSVLQANMRNFAIFIFSFVFFFVPFHLISFAMTLFSKKQLSMYDQLVATLVADKKAT
                     LVFKDVNEETKYRKELAQRLIAVDKRKAESREERLRDKEKLNKID"
     gene            888..1667
                     /locus_tag="PLQ55_00590"
                     /pseudo
     CDS             888..1667
                     /locus_tag="PLQ55_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2341..3444
                     /locus_tag="PLQ55_00595"
     CDS             2341..3444
                     /locus_tag="PLQ55_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPK28471.1"
                     /translation="MSTKAGVLHLEKGPMQTKKTIAMPIWLKAIIALLPALFFLLVFT
                     IFPIFNTAFIAFVKDFYFQSGTGSFALASYFRQLDMHNNPDWWREYFAIIEPDVPFLQ
                     PVKPIIGFANFEYVLKDPLFSNALINTAILTIISVPLTIMISLLLAVSLNSIKPLRAT
                     YQTIFFLPYVTNGIALGMVFNTIFSHEVGGIMNQFIQFFGGKPVSWIRPLWSPEGTIP
                     LSKFSLGFAITVQSIWSGLAFKILVFMAGLASIDKQYYDAARIDGSSRSTIFRRITVP
                     LLSPQILYITITSFIGAFKAYQSVISIVGGGPIHFGGENRTMWITVVGYIYMNRNVEK
                     NIPLAAAGSVVLLIVILFITLIQMQVSKKRVHY"
     gene            3460..4518
                     /locus_tag="PLQ55_00600"
     CDS             3460..4518
                     /locus_tag="PLQ55_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPK28472.1"
                     /translation="MTDKDKKVTEAVELTAEEEVKLSHIDDERFQPSYEIAKEYKTRA
                     IVVKIITYTFLTLFAFFMLLPFWFMISVSVKTPLEYQREVSTGLRLFTANPSFVNFRI
                     VLGMDLKDPLKEMNEALLHIVETSPERSFIPYFLNTIIVAVVSTVFTIVTTVLASFAF
                     ARLEFKGKNFLFAILLATMMIPGEMMLITNIQTAKWLGWENTYRALIFVHGVSVFYIF
                     YLRQTFQQIPNELFLAAKVDGYGEFSYLWRVMIPIGMPTIVTIIILNVMGSWNAFIWP
                     TLIAQGYNPTIQKLFKYNHTMRLVSNGLMSTFTGQFASYDTVKIAASMIVTAPLLLVF
                     VFFRKYIMSGVSRSGIKG"
     gene            4563..6158
                     /locus_tag="PLQ55_00605"
     CDS             4563..6158
                     /locus_tag="PLQ55_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPK28473.1"
                     /translation="MKFRKLIFTAVSVLALTTLAGCLRPPMPPTSETPTTSSGGGGPT
                     EKIEVKFWTGFGASVTGALNPLFARFEQENPDIKVLYESKGGYPNLQQAINGSISTNT
                     YPHIANGYPDHLVGYINANILVNLGNPTNYINHPDYGVDINEYNSDYMAEINGLSDAA
                     TFGLPFNKSTEVMIANQSFFDAAKKRDPSIFIPKTWQDLAEVGPKLKAVAKDNGWFGK
                     LVLKEDGTAVAKPAEFDAALKEQVAVDMTLVNENQFIPFSWDSASNFFITIIRQWGAM
                     YTEKGNTIQNGYIRFQEEPHRTKTLAALSYFKELYEDGLIGLPDNFGEPLYSSTPFKE
                     GKLVLTISSSAGVGENLPGGTTEYPFELSINPIPYNGDLAAQKSVIAQGTNLALFTVG
                     QGRDPKAQAERLAAWRLLRFLTYEANYEFGKATSYFPVLDASKIDRDSQRYQDYLMYS
                     EFLNQTDGTPREKVIRSTAKLQANVYMDPEAGWNKFVDPGFLGSSEIRNTIESVMAEL
                     FKGKTPEQVLNDTVTKLARYVQK"
     gene            6173..7474
                     /locus_tag="PLQ55_00610"
     CDS             6173..7474
                     /locus_tag="PLQ55_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28474.1"
                     /translation="MKKTFVKIGLLLAVFGLLVGCDNSSSEVVPPSSASEEVVSSSEE
                     EVKLTEQEYQIAVNYIARSVPLVLERGVKVPTNVERAEAENPLPTPELKYGDNLLLIT
                     AGELKYEDEINGVYLYPEYTVTYSFLEQEPYAGFTFEYDDIGRYFAVPEYPRYEAEYD
                     NGGNPIHTVPPAKAARLYVTIHIEERFTTFNMDMILEPQAIIHDYNLADVRSVPTGSV
                     VRVYGYFNGYFSDWNNGVLCDGDVALGLFKLQEFNHMELGKMYAVVGQFTVYNGLPQL
                     QWIKTVSEVEPADHPTVKEPKAVVVSADDFADQLDIGNELSGPLMHKVGTTVVLNFPL
                     RYKAMLDRDGNAVEPGALELGKHADVVLTGKDSDNFEFEVKLSLNYHMGTDNQAAFRD
                     FFVTNADADIYFTGPLGAYNAINLAPFAFEGTLSLTPPVVS"
     gene            7563..7638
                     /locus_tag="PLQ55_00615"
     tRNA            7563..7638
                     /locus_tag="PLQ55_00615"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7596..7598,aa:Lys,seq:ctt)
     gene            7746..9155
                     /gene="trpB"
                     /locus_tag="PLQ55_00620"
     CDS             7746..9155
                     /gene="trpB"
                     /locus_tag="PLQ55_00620"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007156746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPK28475.1"
                     /translation="MKYLTVKELSEKWKMSERRIRALIKEKRIEGVKYENKYLIPENA
                     KKPLDRRIKGQRALEDASLNRQDFDFKNYRKHYPTSDGYFGRFGGSFLPKHLQKAIDE
                     VYDGYLTIAKSAKFIAELREIRKNYQGRPTPVYHCLNLSNHLGKVQIYLKREDLNHGG
                     SHKLNNAMGQALLAKYLGKKKLIAETGAGSHGAAVAMAAAYFDLECDIYMGAVDTQKQ
                     RPNVDRIKVLGANVIAVKEGTQTLKEAVDAAFNAYAKEYKNAFYVLGSAVGPHPYPLM
                     VRDFQEIIGREAKEQFVAMTGELPDVVAACVGGGSNAIGMFQAFLEEKVNIVGVEALG
                     QGPTIGQNSAAVSLGKEMILHGFNSLVLQNEDGSVGDAYTIASGLDYPGVGPEHAFLH
                     EIGRVKYEAITDEEAAEAFFLLSRLEGIIPAIESSHAVAYAIKYARSVSAGSILVCLS
                     GRGDKDMEFMTRKYPNMYK"
     gene            complement(9231..9647)
                     /locus_tag="PLQ55_00625"
     CDS             complement(9231..9647)
                     /locus_tag="PLQ55_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28476.1"
                     /translation="MVGITEKLKTPEHSKVTLTGKHNGVDFEQKYTLTDGVWVGDEEY
                     EDPHGYTIKKSVYDHVMTIKSFTVEQQQKYKYFTNSRKFKIEFRDYTSDKDYSYGTFI
                     YNRKGTLMEIHFDSRFVNEEYSINVSDNLLIYYDFS"
     gene            9732..10046
                     /locus_tag="PLQ55_00630"
     CDS             9732..10046
                     /locus_tag="PLQ55_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPK28477.1"
                     /translation="MIVYMNEHFRDPNLTIAKIAESSGYSLSHFSKAFKLYMGHSPIS
                     HLNRLRLSEARYLLHTTHLPFKEIALQCGFKNISTFTESFKQSIGILPKDYRKKYQTP
                     NS"
     gene            10207..>10471
                     /locus_tag="PLQ55_00635"
     CDS             10207..>10471
                     /locus_tag="PLQ55_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018920212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28478.1"
                     /translation="MAIKKKFVSLDGNAAAAEVSYLYTDIAAIYPITPSSPMAELVDL
                     FASQGKLNYFGSKVKVTELQSEAGAAGAVHGALQAGTLATTYTA"
BASE COUNT         3266 a         1940 c         2141 g         3124 t
ORIGIN      
        1 cttacatcat taatgctgtt gaaaagttcc ctaaagcact ctacacattt tacgttgatg
       61 aaaatcttga aacaggcgaa attgaacgcg gttacgctga tatattagtg gaaggtcatg
      121 aatataacac tgcagaagat tattatgtcg ttattttagg gaaaggtcgc gaagactctt
      181 tatttacttt tgacgctatt aatagtgaag cgccgtggga agttcctttt aaagaaggga
      241 agcaagaagc ggtgaagcaa ttttacactg atgaaataga aaaagcgcat caacatcttg
      301 attatcatga agtagtagtt ccgctcatta ttaaagctga acggcttatt ttttatgcaa
      361 tggcgggaag ttacttaaca agtgctttaa ttcttattgt cattattcca aactttagga
      421 aagataaagt aacatttggt aaggtaatta cggggaccat agtcttaaat cgtttaggat
      481 ataaaatgag cgttttgcag gcaaatatgc gcaatttcgc gatatttatc tttagttttg
      541 tctttttctt tgtacccttt catctcattt cctttgcgat gacactcttt tctaaaaaac
      601 aacttagtat gtatgatcag cttgtggcaa cactcgtggc ggataaaaaa gcaacgcttg
      661 tctttaaaga tgtaaatgaa gaaacaaagt atcgtaaaga gctcgcacaa cgcctaattg
      721 ccgttgataa acgcaaagcc gaaagtcgtg aagaaagatt acgcgataaa gagaagctta
      781 ataaaattga ttaaaaacta cataatgccg ataataaaac aatagccaaa aaccttgata
      841 ttgtgctatt ataaaataaa catagcagac aatctgcagg aggaaatatg gaagttagat
      901 tagataatct gtcgaaacac ttccctggag tcaaaggcgc tccgacagtc aaagctgttg
      961 atcagatgaa ttttactatt cctgatggaa agcttgtcgg ccttttagga ccttcgggct
     1021 gtggaaaatc aacgatactc tacgtgatcg ctggattaca caaaccgacc gctggtaaga
     1081 tttggttcgg tgatcgtgat gtcactgagg ttccgcctga aaatagaggg atcggtttag
     1141 tgtttcaaaa ctatgcgctc tatccgcatt ttacagttcg gcaaaacatt acattcccac
     1201 tcgagaatgt cagaccgaaa ctagaaaaaa ccgaaatgga gcaaattgct caagaagtcg
     1261 caaacttagt cgacattggt aacttgctcg accgcctacc aaaacaactt tcgggtggtc
     1321 aacaacaacg ggtagcgatt gcccgtgcgc tctcgaagcg gccccaagtt ttattacttg
     1381 acgaaccgct ctccaaccta gacgcacgct tacggcttca aacccgtgaa gaaatccggc
     1441 ggattcaacg tgatacaaaa gttacaacaa tctttgttac tcacgaccaa gaagaagcaa
     1501 tgagtattag tgactttatc gttgtgatgc gtcgcggtgt tgtccatcaa atcgatgcac
     1561 cgcaaacggt gtatgaagaa ccagtcaact tatttgttgc gaaattctta ggctcaccag
     1621 cgattaatat ttttgatggt gaattaaaga atggtaaagt attacttcac ggcaatgttt
     1681 atcatgaagg gcttaaatca aaaggtaaat ttgattactt tgataaaaga atttatgata
     1741 tcgctgttga aggtggttat aacggaacga aaactgatta cttcgctagc ttaggcgttg
     1801 attatgttga agcagagcca gaagttaaag aagaaccaga aaagaaagca tttaaagaaa
     1861 aagttagtga tttctttgcc aaattaaatc caaagaaaaa acaaaaattt gttccgcgta
     1921 ttattgaagg tgaagttaac cttgaaagca tttatgaagc tatcgtcggt ggcggctatc
     1981 gttatgctta cgctgatttt gaagaagagg ttggtaaact catcgttgaa gctggtgcaa
     2041 atcgtgatgt gaagattggt gtgcgtcctg aagcattcac ccttgacaaa aatgataatc
     2101 cagcaattga tattgaagta ctttatattg aacatattgg ccgcgatatt acgatcgtcg
     2161 ctaatgttgt gggacaaaaa caccggattc gcgttattat ccctgctgaa gaacgtcata
     2221 aagtcaaaga caaagtaatt aaggtatatc caaaacgctt ctatctcttt gatattgatg
     2281 gttcacggat tttataggaa aacgcgatga aagattctta tttacgaaag gaggtaggct
     2341 atgagcacta aagcaggagt actgcatctt gaaaaagggc caatgcaaac gaaaaagacg
     2401 attgcaatgc caatttggtt aaaagcaata attgctttat tacctgccct tttcttcttg
     2461 cttgtcttta ccattttccc gatttttaat acagcattta ttgcttttgt taaagacttt
     2521 tatttccaaa gcgggaccgg tagttttgca cttgcaagtt acttccgaca acttgacatg
     2581 cataataacc ctgattggtg gcgcgaatat tttgcgatta ttgaacctga tgttcccttt
     2641 ttacaaccgg ttaaaccaat tattggtttt gcgaactttg aatatgtttt aaaggatccg
     2701 ctttttagta acgcgcttat taataccgct attttaacaa tcatttccgt cccactgacg
     2761 attatgattt cacttttact tgccgtatcg cttaattcca ttaagccgtt acgcgcaact
     2821 tatcaaacaa ttttcttcct cccctatgtc actaacggca ttgccttagg gatggtcttt
     2881 aatacgatct tctcgcatga ggttgggggg attatgaacc aatttatcca gttctttggc
     2941 ggaaaaccag tatcgtggat tcgtccgtta tggagtccag aaggaacgat cccgttatca
     3001 aaattctcct taggatttgc gattactgtc caatcaattt ggtcaggctt agcgtttaaa
     3061 atccttgtct ttatggcagg tttagcaagt attgacaaac aatactatga cgctgcgcgg
     3121 attgatggct caagtcgcag cacaatcttt agacggatta cggttccact tttaagtccg
     3181 caaatcttat atattacgat tacttcattt atcggggcat ttaaagctta tcaaagcgtt
     3241 atctcgattg tggggggcgg accaattcac tttgggggcg aaaaccgcac gatgtggatt
     3301 acggttgtcg ggtacattta catgaaccgc aacgttgaaa agaacattcc acttgccgca
     3361 gcaggttctg tcgtcttact tattgtaatt ttattcatca cgcttattca gatgcaagtc
     3421 tcgaagaagc gggtgcacta ttaataggag ggtagcgtaa tgacagataa agataaaaaa
     3481 gtcacagaag cagtcgaatt aactgccgaa gaagaagtga aattatcaca cattgatgac
     3541 gaacgctttc agccatccta tgaaatcgca aaagaatata aaacgcgggc gattgttgtt
     3601 aagattatta cttacacatt cttaacgctt tttgctttct ttatgctttt accgttctgg
     3661 tttatgatta gtgtttcagt taaaactcca cttgaatatc aacgtgaagt gtcgactgga
     3721 ttacgcttat ttacggcaaa tccaagtttt gttaacttta ggattgtctt agggatggat
     3781 ttaaaagatc cactaaaaga gatgaatgaa gcactcttac atatcgttga aacttcacca
     3841 gaacgcagtt ttatcccgta ttttcttaat acgattatcg tcgcggttgt ctcaacggtc
     3901 tttacaattg taacaacggt gcttgcctcg tttgcgtttg ctcgtcttga atttaaaggg
     3961 aaaaacttct tatttgcgat tttactcgca acgatgatga ttcctggcga aatgatgctt
     4021 attacaaata ttcaaacagc gaaatggtta ggatgggaaa acacttatcg cgcacttatt
     4081 tttgtccacg gcgttagtgt cttctacatt ttctacttac gacaaacatt ccagcaaata
     4141 ccaaacgaat tattccttgc cgcgaaagtt gacggctatg gtgaatttag ctacttatgg
     4201 cgcgtgatga ttccaattgg catgccgaca atcgttacga ttattattct caacgttatg
     4261 ggtagctgga acgcctttat ttggccaaca cttattgcgc aaggctataa ccccactatt
     4321 cagaaattat ttaaatataa ccatacgatg cgacttgtta gtaacgggtt aatgtcgaca
     4381 tttactggtc aattcgctag ttacgacacc gttaaaattg ccgcatcgat gattgttact
     4441 gctccgttac tgctcgtctt tgtgttcttc cgtaaataca ttatgagcgg tgtctcccgc
     4501 agtggtatta aaggttagta attactaatc gttattatat aaacaagaaa ggaaagagag
     4561 atatgaaatt tagaaaactc atatttacag cggtatcggt cttagcttta acaacgcttg
     4621 ctggttgttt acgcccaccg atgccaccaa catcagaaac gccaacgaca tcttcaggcg
     4681 gtggtggtcc gactgaaaaa atcgaagtga agttctggac aggatttggg gcatcagtta
     4741 ctggtgcatt aaacccgctt ttcgcgcgtt ttgaacaaga aaatcctgat attaaagtac
     4801 tttatgaatc aaagggtggt tatccaaacc tccaacaagc gattaacggt tcaattagta
     4861 caaatactta tccgcatatc gctaatggtt atcctgacca tttagtcggt tacattaacg
     4921 ctaatatttt agtaaatctt ggtaacccca ctaactacat caatcaccca gactatggtg
     4981 ttgatattaa tgaatacaat agcgactaca tggccgaaat taatggctta tcagacgctg
     5041 caacattcgg cttaccgttt aataaatcaa cggaagtaat gattgctaac caaagtttct
     5101 ttgatgcggc taaaaaaaga gacccaagta tctttattcc aaaaacatgg caagatttag
     5161 ccgaagttgg cccaaaactt aaagccgtcg cgaaagataa cggctggttt ggtaaattag
     5221 tactcaaaga agatggaaca gcggtagcaa aacctgcgga atttgatgca gcgcttaaag
     5281 aacaggtcgc tgttgatatg acgctagtta atgaaaatca atttatcccc tttagctggg
     5341 atagcgctag taacttcttt attacaatta ttcggcaatg gggtgcaatg tatacggaaa
     5401 aaggtaacac aattcaaaat ggttacatcc gtttccaaga agaaccgcac cgcactaaaa
     5461 cactagcggc cctatcttat tttaaagagc tatatgaaga tggattaatt ggtctacctg
     5521 acaactttgg tgaaccgctt tattcttcaa caccatttaa agaaggaaaa ctcgtactta
     5581 ctattagtag tagtgccggt gttggtgaaa atttaccagg cgggacaact gaatatccat
     5641 ttgaattatc gattaatcca attccttata acggtgactt agcagcgcaa aaatcagtta
     5701 ttgcccaagg cacaaactta gcgctcttta ccgttggtca aggccgtgat ccaaaagcac
     5761 aagctgaacg gctcgctgcc tggcgcttac ttcgcttctt aacttatgaa gcaaactacg
     5821 aatttggtaa agcaacttcc tacttcccag tgttagacgc ttccaaaatt gatcgcgata
     5881 gtcagcgtta tcaagattac ttaatgtata gtgaattctt aaatcaaaca gacgggacac
     5941 cacgcgaaaa agtaattcgt agtaccgcta aacttcaggc taatgtgtat atggatcctg
     6001 aagcaggctg gaataagttc gttgacccag ggttccttgg ttcaagtgaa attcgtaata
     6061 ccatcgaatc agtgatggct gaattattta aaggtaaaac accggagcaa gtgttaaatg
     6121 acactgttac taaacttgcg cggtatgtac aaaaataata aaggagaaat atatgaaaaa
     6181 aacatttgtt aaaattggct tattacttgc ggtttttggc ttacttgttg gttgtgataa
     6241 tagcagcagc gaagtagtgc caccctcaag cgccagtgaa gaagtcgttt catcaagcga
     6301 agaagaagta aaactaactg agcaagaata tcaaattgcc gttaattaca ttgctcgcag
     6361 tgttccgctt gtccttgaaa gaggcgttaa agttccaaca aacgttgaaa gggctgaagc
     6421 ggagaaccca ctaccgacac cagaattaaa gtatggtgac aacttattac ttattaccgc
     6481 tggtgaatta aagtatgaag acgaaattaa tggcgtctat ctttatccag aatatactgt
     6541 aacctattca ttcttagagc aagaacctta tgcaggcttt acttttgaat atgatgatat
     6601 tggccgttat tttgctgttc ctgaatatcc gcgctatgaa gcggaatatg acaatggcgg
     6661 taacccaatt cacactgtac caccggcaaa agcagcgcgg ttatatgtca cgattcatat
     6721 tgaagaacgt ttcactacat ttaatatgga catgatttta gagccacaag ccattattca
     6781 tgattacaac cttgctgatg tccgtagtgt gccaactggc tcagttgtta gagtctacgg
     6841 ttactttaac ggctatttca gtgactggaa taatggtgta ctgtgtgatg gcgatgtagc
     6901 gttaggtctc tttaaacttc aagaatttaa ccatatggaa cttggtaaga tgtacgctgt
     6961 tgttggtcaa ttcacggtgt ataacggctt accacaatta caatggatta agactgtttc
     7021 tgaagttgaa cctgctgatc acccaacggt taaagaacca aaagctgttg ttgtcagcgc
     7081 agacgacttt gctgatcaac ttgatattgg taacgaatta agcggaccac ttatgcataa
     7141 agttggtacg acagtcgtac ttaacttccc acttcgttat aaggcaatgc ttgaccgtga
     7201 tggtaacgcc gttgaaccag gcgcattaga acttggtaaa cacgctgatg ttgttttaac
     7261 cggtaaagat agtgataact ttgaatttga agttaaactt tcacttaact accatatggg
     7321 tactgataat caagcagcgt ttagagactt ctttgtcact aatgcggatg ctgatattta
     7381 cttcactggc ccacttggtg catataacgc tatcaactta gcaccatttg cctttgaagg
     7441 tacattatcc ttaacccctc ctgtcgtttc ttaaaataaa cgcatgaagc ttggccccag
     7501 actcctgggg cctttttcat ttggcaattt ttataaatag gcgtataatt aaaaagcact
     7561 acggaccgtt agctcagttg gtagagcaat tgactcttaa tcaatgggtc acaggttcaa
     7621 atcctgtacg gttcaccacg ctaaaagaaa gtgcccgatt atgtcgggct tttcttgtac
     7681 ttttttaaag gataggcgta taatttgatt aggaacaata tttataccga agggaactaa
     7741 acagaatgaa atatttaacc gttaaagaac ttagcgaaaa atggaagatg agtgaaagac
     7801 ggatacgagc actcattaaa gaaaaacgga ttgaaggggt aaaatacgaa aataagtatt
     7861 taataccaga aaatgctaaa aagccacttg atcgccggat taaagggcaa agagcgttag
     7921 aagatgcctc actaaatagg caagactttg attttaaaaa ctaccgcaag cactatccga
     7981 cgagtgacgg ctattttggt aggtttggtg gtagcttctt accaaagcat ttacaaaaag
     8041 caatagatga agtgtatgat ggttatttaa cgattgctaa aagcgcaaag tttattgccg
     8101 aactacgcga aattagaaaa aattatcaag gacgaccaac acctgtgtat cattgcctta
     8161 atttatcaaa tcatcttggt aaagtgcaaa tttatttaaa gcgcgaagat ttaaatcatg
     8221 gtggttcgca taaacttaat aatgcaatgg gtcaagcctt acttgcaaaa tatttaggca
     8281 agaaaaagtt aatcgcggaa actggagcgg ggagtcacgg cgcagcggtg gcaatggctg
     8341 cggcttattt tgatttagag tgcgacattt atatgggtgc agttgacact caaaaacaac
     8401 gaccaaatgt tgaccgtatt aaagtgttgg gcgctaatgt gattgcggtc aaagaaggaa
     8461 cacaaacact taaagaagca gtagacgctg cttttaatgc ctacgcaaaa gaatataaaa
     8521 acgcctttta tgtattaggt agtgccgttg ggccgcaccc ctatccttta atggtgcgtg
     8581 actttcaaga aattattggg cgcgaagcta aagaacaatt tgtagcaatg actggtgaat
     8641 taccagatgt cgtcgcagca tgtgttggcg gcgggagtaa cgcaattggg atgtttcaag
     8701 cttttttaga agaaaaagtt aatattgttg gtgttgaagc gcttggtcaa ggcccaacga
     8761 ttgggcaaaa tagcgctgcg gtatcacttg gtaaagaaat gattttgcac ggttttaact
     8821 cattagtgct gcaaaatgaa gacggaagtg tgggagatgc ttacactatc gcgagtggtt
     8881 tagattatcc aggcgttggt ccagaacacg cttttctcca tgaaatagga cgcgtgaagt
     8941 acgaagcaat aactgatgaa gaagcagcag aagctttctt tctcttatcg cgtcttgaag
     9001 gtattattcc cgcgattgaa tcaagtcacg cagtagctta tgctattaaa tatgcgcgca
     9061 gcgtgagtgc tggaagtatt ttagtgtgtt taagtggtcg cggtgataaa gatatggaat
     9121 ttatgacgcg taagtatcca aatatgtata aataataatt tgtgacttaa ttggtgcata
     9181 agaaaaagcc aggtcgccct agctttttag aatcttttaa aattatttag ttaactaaaa
     9241 tcgtaataaa ttaataaatt atcacttaca ttaatgctgt attcttcatt aacaaaccga
     9301 ctatcgaagt gaatttccat tagtgtaccc ttacgattat aaataaatgt tccgtatgaa
     9361 taatctttat cgcttgtata atctctgaat tcaattttaa acttacggga gttcgtaaaa
     9421 tatttgtatt tttgttgttg ctcaactgta aacgatttaa tagtcattac atggtcatag
     9481 acggattttt taatggtgta accgtgcgga tcttcatatt cttcgtcccc aacccacacg
     9541 ccgtcagtca gtgtatattt ttgttcaaag tctacgccat tatgttttcc ggttaaggta
     9601 acttttgaat gttctggcgt tttaagtttt tctgtaatcc cgaccatccg atcaaaatca
     9661 acagggatgt acaggatcaa attatctaat gatcctctaa accaaaagaa tgcgcaacgc
     9721 ggattaaaaa aatgattgtt tatatgaatg aacatttccg tgatcctaat ttaaccattg
     9781 ccaaaatagc ggagtcttct ggttattctt taagtcactt tagtaaagca tttaaactat
     9841 acatggggca ttcgcccatt tcgcatctta atcgtttacg gctttcagaa gcacgctatt
     9901 tattacatac aacacattta ccatttaaag aaattgcgct ccaatgtggc tttaaaaata
     9961 tttctacctt tacggaaagt tttaaacaaa gtataggaat attgcctaaa gattatcgca
    10021 aaaagtatca aacaccaaat agttaataga aaataaaaat aattaagtta gcatttaatt
    10081 gatttatagc gataaactcg tgataaatag gtggttttta tatgtgcgtt ttgtttttat
    10141 aataacgctt acatttttgg ggtttattat ttataataat agagtcatta aaacggaggt
    10201 caaaatatgg ctattaagaa aaaatttgtc tccctagatg gaaacgccgc agccgcagag
    10261 gtaagttatc tctacacgga tatcgcggcg atttatccga ttaccccatc ttcaccgatg
    10321 gcggaactcg ttgatttatt cgcatcacaa gggaaattaa actactttgg cagcaaagta
    10381 aaagtaacag aacttcaaag tgaagctggc gctgctggtg cggtgcacgg tgctttacaa
    10441 gccggaacct tagcgacaac atataccgcg t
//
LOCUS       DAOPCD010000009         9885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_6571_length_9885_cov_4.625839, whole genome shotgun sequence.
ACCESSION   DAOPCD010000009 DAOPCD010000000
VERSION     DAOPCD010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 9885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9885
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6571_length_9885_cov_4.625839"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="PLQ55_00640"
     CDS             <1..1162
                     /locus_tag="PLQ55_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPK28479.1"
                     /translation="VDNSANVQKLNFDDIIGREFKMVHNDDIYKKALGNIDDYVVLDF
                     MGQPRTIEKYRFQNPQYLYDDPTVGEMLKITGILRPKPGIQFEMLAPGMVYTQALNKY
                     MHETNKHNKVAKAQVNNVVIREANITAENINTAIEFYSATTDWSGVKPVNPTTIRIDK
                     FLSNLKIYGVDLADISKDNAIYETLLKELLNTAYSPVESVAIFAKDGDSKKEIKARIN
                     EYNNTREDEKDKIVIFDAVEFVTDSIATMIDVVSIVLIVFTSISLVVSGFLIGIITYV
                     SVIERTKEIGILRAIGARKKDISRLFNAETFIIGLLAGVIGVGTTYILSIPINVILNN
                     LFPEQNIGQIAFLVPLAALALIVFNILLTVTAGLIPSKMAANKDPVVALRTE"
     gene            1182..3668
                     /locus_tag="PLQ55_00645"
     CDS             1182..3668
                     /locus_tag="PLQ55_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPK28480.1"
                     /translation="MEEFVETKKKRPGTATTIIKKSEIKVDEVIRYTPKHDEGLSTQQ
                     VNARKVAKMTNEKPNKGVKSIFSILMRNFFSFFNVLLYAIGAALIYIKEWQSLLFLVI
                     LFSNILIGLIQDFRARRTLKKLLILNEQKVTVVRDGIELLIPSEDVVLDDIVKLKLGD
                     QIAADGVVVHGSVGVNESLLTGEPNIIHKAKDDRVLSGSFVTTGTCYYQVDTIGKLGY
                     AQKLQLRAKVFKRPTSELLNSINSLFKVIGFIVIAMALLLVFNAFYQGSTFEDSVRSI
                     AGSLVAMIPSGMYLLTSMTLAVGVIRLGTKNTLVNEMYSIEMLARVNVLCLDKTGTIT
                     DGTMKVNEVVPLKGAKDIDLNIIVASILRATKDENATAIALKNHFKTRKYMEYTKVLP
                     FDSELKCSGAIMEGKTYIIGASSMVLGPKAQKQVEEVERIYLERGLRVLVLASSRKPF
                     IAKKLPIDMTPLALIVIQDNIRAEASEVIKLFQDAGVEVKIISGDDPVSVSEIARQVG
                     INNAENLLDFSKIDDPSHHSLEDFTIYGRVRPEQKEGIVTKLQSDGKVVAMVGDGVND
                     VLALKAAQCSIAMGAGSPAARGVSHLVLLDNNFDVLPSIVDEGRRAINNLQKTWSLFL
                     VKTIFAIIFSFMFVTAGLLGPKGSGIRYPFEPKHLYLWELLSLGIPAFFLSMQPNKER
                     VRGTFMDNVISRSVPAGLLMVAGAFLLYISKNFGEMFGAEISDTSMEMITMSILTITF
                     LSFVILYRNSLPFNRYRLTLFLVLSLLAIGVIILDNTVGLTFTEKYFLEINISSLEQI
                     HYAILTPINLILTIMYILVDNKIVKKLRRE"
     gene            3672..4589
                     /locus_tag="PLQ55_00650"
     CDS             3672..4589
                     /locus_tag="PLQ55_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HPK28481.1"
                     /translation="MKIRISQEVAYALANNLPVVALESTIISHGMPYPQNVEIALKVE
                     EIVRANGALPATIAIIGGDVVVGLDEDEIEELGQRKDVIKVSRRDLAYVMATKKWGAT
                     TVAATMVIAAKVGIKVFVTGGVGGVHRGATETLDISSDLDELARTNIAVVCAGPKAIL
                     DIEKTLEYLETKGVPVLGYKTDKMPVFYTTSPSLNVLYRVDSSSEIASIVNASHALGL
                     ESGTLVVNPIPKEEALPYKDIEKVIDEALEEMKKAGVKGKEETPYLLSKLNELTKGKS
                     QAANEALVINNAKLGASIAKHICRELYED"
     gene            4579..5124
                     /locus_tag="PLQ55_00655"
     CDS             4579..5124
                     /locus_tag="PLQ55_00655"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HPK28482.1"
                     /translation="MKTKARLGNSLKKFMATMPLDTISVINITKDCKVNRQTFYYHFR
                     NIYDLLTWVFLNEEIEKPSNNELEEAIFIVLRYVKHNEAMIENTLASAAKDLVREFLF
                     NYLYNVCLNYIDKKDPFEKLTLNERKLLARVYGAGIASSIISWIEGEYLFPEHEFVMN
                     LVTFFNDFPLDAINYKGHGYA"
     gene            5117..6289
                     /locus_tag="PLQ55_00660"
     CDS             5117..6289
                     /locus_tag="PLQ55_00660"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPK28483.1"
                     /translation="MHSFNLKIQTELIFGNGRHTEVGSIIKRYGKKNVIILYGQNHAV
                     RSGLLEAVTNALDAKNIDYVLYGGITPNPEVFYVREAKAIAKEYAVDFILAIGGGSVI
                     DVAKSLSVNFYYEGDALDFSKRIVTPKKALPLGVILTHASAGSEMSASCVISDSTNNF
                     KQGFRNDLVRPLFAITNPELTYSVSMYQTAVGIVDTFMHSLERYFNKSDKIELADRFA
                     EAIFVTLRECAETLIADPLDETARANLMLASTFSHNDVTSMGKKANLVVHTLEHALSA
                     LYPSIAHGHGLAVLYPAWLFVYFEELKEKLDRFARVVFNLHHEDIDTNAKEGILALKE
                     LFMRLGLTTTLSSLGVKREDIPTLADIVTENGTKELYHDQKNLTRDDIIKLYESCF"
     gene            6308..7429
                     /locus_tag="PLQ55_00665"
     CDS             6308..7429
                     /locus_tag="PLQ55_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28484.1"
                     /translation="MKKEDLLSILVYLVMLIIALFIGLRIIQPALDALDLVTDLQRYG
                     FAILTILVGIIINVILFELGHVFGALLGGYSVISVNILGLAYYKTKSGWKFSFRRYEG
                     LTGETKIIAKSDKTKPRLYLFGPTIMVLLEFVVAIVIFLLTKYNQPIHHQVLIVAGIG
                     AMLLVYNIMPFKLDNFTDGYYIVLLGKKVNVEAYNELIRIESLVYNNEKVTDIKEFDE
                     VTTMTARLSLYRLYQLIDEKAWDKALSLIDDLEKNASKVEHEFIARIRAQKLYIYLLT
                     KASEAASAYWFDELSAADRKFISNDLTIETMRVYLLYSGLVTKSQSECAFVLSRAPKA
                     LRARINDFRKEEEIALFNEAYEKIVALPGNENLKLPVLK"
     gene            complement(7487..7996)
                     /gene="mscL"
                     /locus_tag="PLQ55_00670"
     CDS             complement(7487..7996)
                     /gene="mscL"
                     /locus_tag="PLQ55_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPK28485.1"
                     /translation="MRKFFKEFKAFIARGNVLNLAVGIIIGGAFNAIITSLVNHVIMP
                     PISLLLGNEVSLLKWVIRPEVRDAADNVVTAEIAISWGLFVQAIINFLLIALVLFVIV
                     RVVTRLTEKAEALRAKLLHEEAAAAPEPEPEPEPEPSEEVLLLREIRDSLVAKKQEAK
                     ATKKKEPKK"
     gene            8172..9026
                     /locus_tag="PLQ55_00675"
     CDS             8172..9026
                     /locus_tag="PLQ55_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPK28486.1"
                     /translation="MPKFKIVVDSTIDLSPEQYKKLGAIVIPLNVHIGEETFQDGVDI
                     NADKLYEKVAETKTAPKTSAVAPGVFKEVFEKLKAEGYTGALFVGIGESLSATAQSVR
                     LAAEEVKDFKVRVVDSHSLSTGSGLLALRAYDLLNEGKTLDETADYLEKIAPKSRAQF
                     IVDNLEMLAAGGRVTGIKLFFGRILRAHPFLQVNNDKLEVVATPKGKSERALDLMLQV
                     AREEISAGLESPRVFITHSHGGERIDYLYDRLKEFVDPKNILISEAGAVISSHCGAGT
                     IGILYIRK"
     gene            complement(9071..9448)
                     /locus_tag="PLQ55_00680"
     CDS             complement(9071..9448)
                     /locus_tag="PLQ55_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPK28487.1"
                     /translation="MRLTPARKLVLNLFKTKSSLLCAEDIERLLKNESIFLSTIYRAL
                     EYFEKENVLGKSTINKTAYYYLIDGKHHHYMICTTCLARFPIDCKLDSMINKNMKKHN
                     FVPTSHDLTIYGYCENCAKLQNN"
     gene            complement(9426..>9885)
                     /locus_tag="PLQ55_00685"
     CDS             complement(9426..>9885)
                     /locus_tag="PLQ55_00685"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPK28488.1"
                     /translation="TVPEIIGASVLALLVICFILIFYRKLIITTFDVTHARFAGVNTM
                     FLKYALSALTSIFVVVGVRAIGALLISAFILFPTLISRQLSKSFNMTLWGGVIISLIA
                     TFIGITVSYHFTLPTGPAIIMVFTTFLIVAYIIRIIIAKRRLKDAPNARS"
BASE COUNT         3371 a         1580 c         1951 g         2983 t
ORIGIN      
        1 tgttgataat tccgcaaatg tacaaaagct taattttgat gacattattg ggcgagaatt
       61 taaaatggtg cacaacgatg atatttataa aaaggcattg ggtaatattg atgactatgt
      121 agtgcttgac tttatggggc aaccacgcac gattgaaaag tatcgttttc aaaacccaca
      181 atatttatat gatgacccaa cggttggtga aatgctaaaa attactggca ttttgcggcc
      241 aaaaccggga attcagtttg aaatgctcgc gccaggaatg gtgtatacac aagcattaaa
      301 taaatatatg catgaaacaa ataaacataa caaagttgca aaggcacaag ttaataatgt
      361 agtaatcaga gaagcaaata tcactgcaga aaatattaat actgcaattg aattttatag
      421 cgctactacc gattggtcgg gtgtaaaacc agttaatcca acaacaataa ggattgataa
      481 atttttaagc aacctcaaaa tttatggagt ggacttagcg gatataagta aggacaacgc
      541 gatttacgaa acactattaa aagaattatt aaatacagct tattcaccag tagaatcagt
      601 agcaattttt gctaaagacg gtgattcaaa aaaagaaatt aaagcacgga ttaatgaata
      661 taataatacg cgcgaagacg agaaagataa aattgtaatc tttgatgcag tagagtttgt
      721 tactgattca attgcgacaa tgattgatgt tgtttcaatt gtattaattg tatttacaag
      781 tatctcactt gttgtgtcag gattcttaat tggcattatt acttatgtta gtgtgattga
      841 aaggacaaaa gaaattggga tactgcgtgc gattggggcc aggaagaaag atatatcgag
      901 gctatttaac gcggaaacgt ttattattgg actacttgcg ggggtaattg gcgttgggac
      961 aacatatata ttatcaatac cgataaatgt tattcttaac aatctatttc ctgaacaaaa
     1021 cattggacaa atagcgtttt tagtaccact tgcagcttta gcgcttattg tatttaatat
     1081 attattaacc gtcaccgctg gattaattcc aagtaagatg gcggcaaaca aagatccagt
     1141 cgtagcgctg cgcactgagt aatgaataga aaggactcat tatggaggaa tttgtagaaa
     1201 caaagaaaaa aagaccgggt acggcaacaa caatcattaa aaaaagcgaa attaaagttg
     1261 atgaagtaat tcgttataca ccaaaacacg atgaaggttt aagtacccaa caagttaacg
     1321 cccgcaaagt agcgaaaatg actaacgaaa aaccaaataa aggcgttaag tcaatctttt
     1381 caatattaat gcgtaatttc ttttcgttct ttaatgtttt gctctacgca ataggagcag
     1441 cgcttattta tattaaagag tggcaaagtt tgttatttct cgtcattctt ttttccaaca
     1501 ttttaatcgg acttatccaa gactttaggg cgcgacgcac actaaagaaa ctcttaatat
     1561 taaatgaaca aaaagtaacg gtcgtgcgcg atgggattga gttattaatt ccaagtgagg
     1621 atgttgtact tgatgatatc gttaaattaa aattaggtga tcaaatcgca gcagatggtg
     1681 tggtagtgca cggtagtgtt ggtgttaatg aatcactatt aactggtgaa ccaaacatta
     1741 ttcataaagc aaaagatgac cgcgttttgt cggggtcttt tgtcacaaca ggcacatgtt
     1801 actatcaagt tgatacaatt ggaaaattag gttatgcaca aaaattacaa ttacgggcga
     1861 aagtatttaa gcgtccaaca tctgaacttt taaattcaat taattcacta tttaaagtaa
     1921 ttgggtttat tgtgattgct atggcattac tattagtgtt taatgccttt tatcaaggct
     1981 caacatttga agatagtgtg cgctctattg ccggttcact tgtggcaatg attccttctg
     2041 ggatgtattt attaacaagt atgacgcttg ccgtaggagt tattagactc gggacaaaaa
     2101 acacacttgt gaacgaaatg tattcgattg aaatgttagc aagagttaat gtactctgtt
     2161 tagataaaac aggcacgatt actgacggca caatgaaagt aaatgaagtt gtgccactta
     2221 aaggcgcaaa agacattgat ttaaacatta ttgttgcctc aattttaaga gccactaaag
     2281 atgaaaacgc cactgctata gcactcaaaa accattttaa aacgagaaaa tatatggaat
     2341 acaccaaagt gttaccgttt gatagcgaac taaaatgtag cggtgcaatt atggaaggta
     2401 aaacttatat cattggtgct tcaagtatgg tgcttggtcc aaaagcacaa aaacaagttg
     2461 aagaagttga acgcatttac ttagagcgcg gcttacgtgt attagtactt gcttcgtctc
     2521 gcaagccgtt tatcgctaaa aaactaccaa ttgatatgac ccctttagca cttattgtta
     2581 ttcaagataa tattcgcgct gaagctagtg aagtcattaa actattccaa gatgctggtg
     2641 ttgaagtaaa aattatttcc ggagacgatc ctgttagcgt gagtgaaata gcgcgtcaag
     2701 tcgggattaa taatgccgaa aatttattag acttttctaa aattgatgat ccaagtcacc
     2761 actcacttga agactttaca atttacggca gagtgcgtcc tgaacaaaaa gaaggtattg
     2821 taactaaatt acaaagtgac ggtaaagttg tagcgatggt tggggacggc gtaaatgatg
     2881 tcttagcatt aaaagctgcg cagtgttcaa ttgcaatggg cgctggtagt ccagcagcac
     2941 gcggagtcag tcacttagtg ctccttgata ataactttga tgttttgcct tcaattgttg
     3001 atgaaggacg aagagcaatt aataacttac aaaagacatg gtcattattc cttgttaaaa
     3061 caatcttcgc gattatcttc tcgtttatgt ttgttactgc gggcctttta ggaccaaaag
     3121 gatcaggcat tagatatccc tttgaaccaa aacacttgta tttgtgggaa ttattatcat
     3181 taggaattcc cgccttcttc ttatcgatgc aaccgaataa agaaagagtg cgcgggacat
     3241 ttatggataa tgtcattagt cgcagcgtgc cagcaggatt gttgatggtc gctggagcat
     3301 ttttactcta tataagtaaa aactttggcg aaatgtttgg tgcagaaata agtgatacat
     3361 caatggaaat gattacaatg tcgattctta caattacatt cttgtcattt gttattttat
     3421 atcgaaactc attaccattt aatcgttatc gactgacact tttcttagta ctaagccttt
     3481 tagcaattgg tgttattatt ctcgataaca ctgtcgggtt aacatttacg gaaaaatatt
     3541 tcttagaaat aaacatctca agtttagagc aaattcacta tgcaatatta actccaataa
     3601 atttaatact tacaataatg tacatattag tggataataa aattgtaaag aaattaagga
     3661 gagaataatt tatgaaaata cggattagcc aagaagtagc ctacgcttta gcgaacaact
     3721 taccagttgt agcgctagaa tcaacgatta ttagtcacgg gatgccttac ccgcaaaatg
     3781 tggaaatagc gcttaaggtt gaagaaattg tgcgcgctaa tggggcgcta ccagcaacaa
     3841 tagcgattat tggtggtgac gttgtcgttg gtttggatga agatgaaatt gaagagttag
     3901 gccaaagaaa agatgtcatt aaagtttcac ggcgtgacct agcctatgtt atggccacaa
     3961 agaagtgggg tgcgacgacc gttgctgcga ccatggttat tgctgctaaa gttggcatta
     4021 aagtatttgt aactggtggc gttggcggag tgcatcgcgg tgcgactgaa acactagata
     4081 tttctagcga ccttgatgaa cttgccagaa caaacattgc ggtcgtatgc gctggtccaa
     4141 aagcaattct tgatattgaa aaaacattgg aatatttaga aactaaaggt gttccagttt
     4201 taggttataa aaccgacaaa atgccagtat tttacacaac aagtccatca ttaaatgtct
     4261 tatatcgcgt tgattcttct agcgaaattg cttcgattgt taacgcaagt catgccttag
     4321 ggcttgaaag cggaacactt gttgttaacc caattccaaa agaagaagcc ttgccttata
     4381 aagacattga aaaagtaatt gatgaagcgc ttgaagaaat gaaaaaagct ggggttaaag
     4441 gtaaagaaga aacgccttac cttctttcca aactaaacga attaacaaaa ggtaagagtc
     4501 aagccgcaaa cgaagcactt gtcattaata acgctaagtt aggagcgtca attgctaaac
     4561 atatttgccg ggagttatat gaagactaaa gctcgtttag gaaattcttt aaaaaagttc
     4621 atggcaacaa tgccacttga tacaataagc gttattaaca tcacgaaaga ttgtaaagtt
     4681 aatcgccaaa ctttctatta tcattttcgt aatatttatg atttattaac atgggtattt
     4741 ttaaatgagg aaattgaaaa accatctaac aatgaattag aagaagcaat ctttattgtg
     4801 ctgcgttatg ttaaacataa cgaagcaatg attgaaaaca ctttagcgag cgctgctaaa
     4861 gatttggtaa gagagtttct ttttaattat ttatataatg tgtgtttaaa ttacattgat
     4921 aaaaaagatc catttgaaaa attaacatta aacgaacgaa aacttttagc aagagtatat
     4981 ggcgcaggta tcgctagtag cattatttcg tggattgaag gcgaatactt attccctgaa
     5041 cacgaatttg tgatgaattt agtaacattt tttaatgact tcccgcttga cgctataaat
     5101 tataagggac acggctatgc atagttttaa cttaaagatt caaactgaac tcatttttgg
     5161 taacggccgg catactgaag taggcagtat aattaagcgt tacggcaaaa agaatgtaat
     5221 tatattatat ggtcaaaatc acgcagtaag aagtgggctt ttagaagcag tgactaacgc
     5281 acttgatgct aaaaatattg attatgtact ttatggcggg attacgccta atccggaagt
     5341 cttttatgtg cgtgaagcca aagcgattgc aaaagaatat gcagtagact ttattttagc
     5401 aattggtggt ggcagtgtaa ttgatgtcgc aaagtcacta tcagtgaatt tttattatga
     5461 aggcgatgca ctagatttta gtaaacggat agttactcct aaaaaagcat taccgcttgg
     5521 tgtaatctta acccatgctt cagcgggtag tgaaatgtcg gcgagctgtg taattagtga
     5581 tagcactaat aattttaaac aagggtttag aaacgattta gtaagaccgc tttttgctat
     5641 tactaatcca gaattaactt atagtgtctc aatgtatcaa acagcagtgg ggattgttga
     5701 tacatttatg cattcgcttg aacgctattt taataaaagc gacaaaattg agttagccga
     5761 ccgctttgca gaagcgatat ttgttacttt acgggaatgc gcagagacac ttattgctga
     5821 cccacttgat gaaacggcgc gcgctaattt aatgttagcg agcactttta gtcacaatga
     5881 tgtaacaagc atgggtaaaa aagctaattt agttgtccat actttagaac atgcacttag
     5941 tgcgctttat ccaagtattg ctcatgggca cggtttagcg gtgctctatc ccgcatggct
     6001 ctttgtttat tttgaagaat taaaagagaa actcgatcgt tttgcgcgtg ttgtttttaa
     6061 tcttcatcat gaagacattg atacaaacgc aaaagaaggc attttagcac ttaaagaatt
     6121 atttatgcgt ttaggattaa caactacttt atctagttta ggtgttaagc gtgaagatat
     6181 ccccacttta gcggatatcg taacggaaaa cggcacaaaa gagttgtatc acgaccaaaa
     6241 aaatctcaca agagatgata taatcaaact ttatgaaagt tgtttttaaa actgttggag
     6301 gacgaatatg aaaaaagaag atttgcttag catattagtg tatcttgtca tgctaattat
     6361 tgcgttattt attgggttaa gaattattca acctgcgctt gacgcacttg atttagtgac
     6421 tgatttacaa cgctacggat ttgcgatatt aacaatcctt gttggtatta ttattaatgt
     6481 tattttattt gagttaggcc atgtctttgg tgcgcttctt ggtggctata gtgtgataag
     6541 tgtaaacatc cttggattag cttattacaa aacaaaaagc gggtggaagt ttagctttag
     6601 acgttatgaa ggattaacag gcgaaacaaa aattattgct aaaagcgata aaacaaaacc
     6661 acggctttat ttgtttggtc caacaataat ggtgcttttg gaatttgtgg ttgcaattgt
     6721 tattttctta ttaacaaagt ataatcaacc tattcatcac caagtgttaa tcgttgcggg
     6781 gattggtgca atgcttctag tctataacat catgcctttt aaacttgata acttcacaga
     6841 cggatattac attgtgcttc ttggcaaaaa agtaaatgtt gaagcgtata acgaattaat
     6901 tagaattgag tcgctcgtgt ataacaatga aaaagttacg gacattaaag agtttgacga
     6961 agtcacaaca atgacagcac gtttaagttt atatcgctta tatcaattaa ttgatgaaaa
     7021 agcgtgggac aaggcattat cattaattga tgacttagaa aagaatgcga gtaaagttga
     7081 acatgaattt attgcgagaa ttagagcgca aaaactttat atttacttat taacaaaagc
     7141 tagtgaagca gcaagtgctt attggtttga tgaactaagc gccgcagatc gtaagtttat
     7201 tagtaacgac ttaacaattg aaacaatgcg tgtgtatctt ctctatagtg ggttagttac
     7261 taagagccaa agtgaatgcg cctttgtttt aagtcgtgct cctaaagcac tgcgcgctag
     7321 aataaacgat ttccgtaaag aagaagaaat agcgcttttt aatgaagcgt atgaaaaaat
     7381 tgtggctttg cctggtaatg aaaatctaaa acttccagta cttaaataaa ataaaaaaca
     7441 ccagtaaatg tttacttaga aactagaaac tggtgttttt cttttactac tttttaggtt
     7501 ctttcttttt agttgctttt gcttcttgtt ttttggccac taatgagtca cgaatttcac
     7561 gaagtaataa tacttcttcg cttggttctg gttctggctc tggctctggc tctggagcag
     7621 ctgcagcttc ttcgtgaaga agtttagcgc gtaaagcttc tgctttttca gtaagtctag
     7681 ttacgacacg aacaatcaca aataaaacaa gagcaattaa taagaagtta ataattgctt
     7741 ggacaaataa accccatgaa atagcaattt ctgcagtcac aacattgtca gcagcgtcac
     7801 gaacttctgg cctaataacc cattttaata gtgaaacctc attaccaagt aataacgaga
     7861 taggcggcat aattacatga tttactaaac tagtaataat tgcgttaaaa gcgccaccga
     7921 tgataatacc aacagcaaga tttaagacat taccgcgggc aatgaacgct ttaaattctt
     7981 taaaaaactt tctcataatt tatttccttt ctgctttcaa ttttagcata aagctaaaag
     8041 taagtagtaa aacaaataag accgtagtac tttaaatatg caaaataaaa taaaataaga
     8101 tattattgat agtgaagtca cttaaaagtg atgattaaag agttgtgagc aaatcacaaa
     8161 aaggagaaaa tatgcctaaa tttaaaatag tcgttgattc aacaattgat cttagtcctg
     8221 aacaatataa aaagcttggg gcgattgtta taccattaaa cgttcatatt ggtgaagaaa
     8281 ctttccaaga cggcgtcgat attaacgctg ataaacttta tgaaaaagta gcagaaacga
     8341 aaacagcacc aaaaacgagc gctgttgccc ctggagtatt taaagaagtg tttgaaaaat
     8401 taaaagccga aggttataca ggcgctttat ttgtggggat tggtgaatcc ctaagtgcaa
     8461 cagcccaaag tgtgcgttta gcagcagagg aagttaaaga ctttaaagtc cgagtcgtcg
     8521 attcacactc actttcaaca ggaagcgggt tattagcgct acgcgcttat gaccttctta
     8581 acgaaggcaa aacacttgat gaaaccgctg attacttaga aaaaatcgcg cctaaatcaa
     8641 gagcgcaatt cattgttgat aatttagaaa tgctcgctgc tggtggaaga gtaacaggca
     8701 ttaagttatt ctttggtcgt atactacgtg cccacccatt cttacaagtt aataatgaca
     8761 agttagaagt agtagcgaca ccaaaaggta aaagtgaaag agcattagac ctaatgctac
     8821 aagtagcacg cgaagaaatt agtgctggct tagaatcacc acgcgtcttt attacgcact
     8881 cacacggcgg tgaacgcatt gattatcttt acgaccgact aaaagaattt gtcgatccca
     8941 aaaatatttt aattagtgaa gcaggcgccg ttatttcaag tcattgtggc gcaggcacaa
     9001 ttggtatttt atatattcgt aaataacaat aattaattac taatgaaatg ccgcttaatc
     9061 gcggcatttt ttagttgttt tgcaactttg cacaattttc acaatacccg taaattgtta
     9121 gatcgtgact tgtgggaaca aaattatgtt tcttcatatt tttgttaatc atactatcta
     9181 atttacaatc aatagggaat cgcgcaagac aagtagtgca aatcatgtaa tgatgatgtt
     9241 taccgtctat taagtaatag taagctgttt tattaatagt gctcttgcct agaacatttt
     9301 ctttttcaaa atattctaaa gcacgataga ttgttgaaag aaaaatagat tcatttttta
     9361 ataatcgttc aatgtcttcg gcacaaagta atgaagattt tgttttaaat aaattaagca
     9421 caagtttacg agcgggcgtt aggcgcatct ttaagtctcc ttttagcaat gataatacga
     9481 ataatatacg ctactatgag aaatgtagta aataccataa tgattgcggg tcctgtcggc
     9541 aaagtaaagt gataagaaac agtaatccca ataaaagtag cgattagtga aataataacg
     9601 ccgccccaca aagtcatatt aaaactttta gataattgtc tagagattaa tgttggaaac
     9661 aaaataaatg cagagattag tagtgcccca atcgcgcgga caccgacaac aacaaaaata
     9721 gaagtaagtg cggaaagggc atatttaaga aacatagtgt taaccccagc aaaacgcgca
     9781 tgagttacat caaaagtcgt aattataagc ttacgataga atattaagat aaaacaaata
     9841 acgagcaaag ctaaaactga agcaccaata atttcaggaa cagta
//
LOCUS       DAOPCD010000010         9025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_7260_length_9025_cov_4.258082, whole genome shotgun sequence.
ACCESSION   DAOPCD010000010 DAOPCD010000000
VERSION     DAOPCD010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7260_length_9025_cov_4.258082"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="PLQ55_00690"
     CDS             complement(<1..1181)
                     /locus_tag="PLQ55_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28489.1"
                     /translation="MKIITLFKVIANLYKRNIKKNRLMVIFMIFTNITLMLFLVFFSS
                     SETLAQTLAEGYLEYLSVDVALYESQPTNNPLINVKSYRRPSIDYLNSIMKVTESFVI
                     RPDYSPLLYEKEIMVLGKEIPNVLITIIGENTNEIGINRAFYNELNKLVSIKDDEMLL
                     HFYIKTTIFIDDEEVLIDYYNQFPITYIYDEPTYFSVPKLYISQNTIDATLGNIFLKP
                     GLTLNNYLLNLNPEHELSNYRFKCHFKSAAQKDKFMHIVNEVSNNESGIEVSGDHINK
                     IESFNALYDYLSILMKLFLAFVIIGSVVIYVVIAHTALLTILRQMALFAVLGASKKQI
                     YEIYLFLIIINFLLSSVSFLLLPSLLPVVSFILHKLMGVYVVLELNYTMLVLMLSADL
                     LT"
     gene            complement(1159..1737)
                     /locus_tag="PLQ55_00695"
     CDS             complement(1159..1737)
                     /locus_tag="PLQ55_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK28490.1"
                     /translation="MFSNLSYTFKSGKIYVIVGYSGSGKSTFLHIVANYISKNSGKIK
                     YKNKLITKNDVTLVLQHPYFLGDLRIIDNLKISSLFRHLNNRKIRQKYTKALQISHIL
                     QRRANVCSGGEKARANILRGIIERKHILLIDEPTAHLDFKNSQIVAKLLSELSANKIV
                     IVTTHQPSLFKSGNTIFLRIKNGVFHEDNNII"
     gene            complement(1811..2029)
                     /locus_tag="PLQ55_00700"
     CDS             complement(1811..2029)
                     /locus_tag="PLQ55_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HPK28491.1"
                     /translation="MALIKVDLYDEIEMKKPHPCIHKSHRFQVVMLGADLKLQCQGCG
                     NIVLISRIKFNQKFKKHIASHETIIFTK"
     gene            complement(2029..3633)
                     /locus_tag="PLQ55_00705"
     CDS             complement(2029..3633)
                     /locus_tag="PLQ55_00705"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPK28492.1"
                     /translation="MKNTLHILEVAKKVGLSPADVILYGEDKAKIKSPFTDINPQGKL
                     ILVTAITPTPFGEGKTTMSIALQDAMWKTGTKSLLCLREPSLGPVFGIKGGATGAGRA
                     KLYPSDDINLHFTGDLHALTSSINLIAAVIDNHIFQGNALNLDPKRITWKRSLDMNDR
                     GLRKVRINQGTGNGTPRCEEFQITVASELMAIMCLASDPDDFIRRVKDIIIGYTYEGE
                     IVRLAALEIEDAIMHLMKQALLPNLVQTLEGNPVLVHGGPFANIAHGCNSIIATKYAL
                     SKAPVVITEAGFASELGAEKFFNIKCRTANLKPNLAVVVATIKALKMHGGVPMSAIYE
                     ENTDALAKGFENLVQHYENLSLFGVKVLVALNLFDNDHKSEVKLFEKEMQKLGYDYAL
                     VSSVSKGSEGGIEFANKVKALLAEETKFEFLYDTEKEVEEKIEIICKKLYRASGVKYS
                     KRAQRELAVIKEQGFAKLPVCMAKTPLSFSDNPNLKNAPRNFNITIREFRLSAGAGFI
                     VALTGKVLTMPGLPKVPAAVNMKEVR"
     gene            complement(3630..4511)
                     /gene="miaA"
                     /locus_tag="PLQ55_00710"
     CDS             complement(3630..4511)
                     /gene="miaA"
                     /locus_tag="PLQ55_00710"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPK28493.1"
                     /translation="MIFVIGGVTASGKSDLAFRFAKAINGIIINADAFAFYKELNIGT
                     AKPTKEELAMLPTFLFNNISLDERFSIYNYQKEVRIILDKYLAKKIPIVLVGGSGLYI
                     RAALYNYNLQPEKDLKIVDDPNISNTSLHKELEVLDKEAAAKIHPNNRKRVLRALALI
                     KTHAQTKTALEEVTSSEPLYPYIMICLDKENEMLHRDIETRVKLMFDKGLREEAMTLS
                     KKYPRNAQGMQAIGYKEIVQNPNATDEELINLIALRTRQYAKRQRTFFRNQFAAKWFT
                     DKEAAFNYLRGKYEDSL"
     gene            complement(4508..6262)
                     /gene="mutL"
                     /locus_tag="PLQ55_00715"
     CDS             complement(4508..6262)
                     /gene="mutL"
                     /locus_tag="PLQ55_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPK28494.1"
                     /translation="MAKIILLDNELANQIAAGEVIERPSSVVKELVENSIDAGATKID
                     VFINEAGRKAIIVQDNGSGIPSDELALAFSRHATSKIHSTFNLFRIRTLGFRGEALPS
                     IASIAKVQVETAEKGKPGALAKIENGDIKIEPSAVSSGTKITVSELFYNTPARLKYLK
                     RDYTENAVTLDVVTKIALAHPDVSFSLTFDDKLRFRTSGRNNLEETILNIYGLTYVKN
                     LLPISVKTNDFTITGFLGNTNLVRSNRYGIITNLNGRNVYMPKVQSTIIEAYHGFIAP
                     TRYPFVILNLQIEPELVDVNVHPTKKEVRFSKEEDLRNVLLKEIPLALRKEAFIPKDP
                     HQKVQPLSSTTNEEPRAEQLIFDLPFGTETSELKISDSHHQEAIRKPITKTISELENS
                     FVNPVAALHNTYIICEVNDGGFWLIDQHAAHERINYEKFQKVLNMDLKTRPLLVPELL
                     SFSHSEIALFTPEVFTILKDVGIEAELFGPNTLRVTAVPSWAGEYDERIYIQDIIDAV
                     ITDKRLNQELLRTNAIATMACKASIKANHRLSIMEMQHLIKELLACDNPFSCPHGRPT
                     IIKFTKYELEKMFKRTGT"
     gene            complement(6262..8793)
                     /gene="mutS"
                     /locus_tag="PLQ55_00720"
     CDS             complement(6262..8793)
                     /gene="mutS"
                     /locus_tag="PLQ55_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPK28495.1"
                     /translation="MGKEKKKIVYTPMIEQYLEIKRENPGILIMYRLGDFYEFFFEDA
                     EIVSKELQLVLTKRAAGNNRKIPMCGIPHHAYGPYLAKLIDAGHKVGIVEQMEDPSSA
                     KGIVKREIIQIVTPGSIVDFEDSDNNFITAVDAYADAFTVSYADVSTGDIFVTTLPRE
                     DTSLVSYLNGLNTREIVVSPQFSDKLLGVIKTNINLVISFEENTKIDEEYEDLFSDVK
                     DEREYLIAARLVSYLTRTKKRQLSYFKAITVTKPQSFLQIDAFSRLNLELTRTIRNET
                     KYGSLFWLLDETTTAMGRRKLKDFIIRPSHDYDEIIRRQNIVNTFIVNFVTRAEVKEH
                     FVSIYDLERIVARISFGSANPRDLLRLKTSLQALEKARNLLGKIREPEISELYDKLAS
                     NIPLITLLEKAISEDAPLQITGGEIFKYGYYEALDELIDLAHGGKKWISAFEENERVR
                     TGIKNLKIGYNRVFGYYIEVSNSNLDQVKDEFGYIRKQTTKNGERFINEALKEQETLI
                     LSAEERRQQLERELFGALLNELKKSTRLLQQSADVVSEIDVFISLAEVAVANNYVRPI
                     FNDARVVDIKEARHPVLSKLLKDHAFVANDIELDEATDIVIITGPNMGGKSTLMRQLA
                     LIVIMAQIGSFVPAKSANLMIFDQIFTRIGASDDLISGQSTFMVEMIEANYALRKATP
                     NSLLLFDEIGRGTSTYDGMALAYAMLEYIANKLKVKTLFSTHYHEITAIDEHYPNIKN
                     IHVEVKEVENNVTFLYKIKPGPMNKSYGINVARLAGLPLEILSNAQEHLQTFVANNSG
                     GNPTKTEYVPLKPLWYQQLENLNIDEMTPLDALILLKKIKDEGFK"
     gene            complement(8824..>9025)
                     /locus_tag="PLQ55_00725"
     CDS             complement(8824..>9025)
                     /locus_tag="PLQ55_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28496.1"
                     /translation="KETETKLKELQQKMTQNMGDKVVHKKYQEEYLVLKKAYENHPYI
                     LNYNSLHAEVEELLYTLKKVIE"
BASE COUNT         2825 a         1698 c         1360 g         3142 t
ORIGIN      
        1 gttagaagat cagcgctaag catcaacact aacatcgtgt aattaagttc aagcacaaca
       61 taaacaccca tgagtttatg taaaatgaaa cttacaacgg gtagtaaaga aggtaaaagc
      121 aagaaactta ccgagcttaa taaaaaatta ataataatca gaaataaata tatttcgtat
      181 atttgctttt tactagcgcc taaaacggca aaaagcgcca tttgtcgtaa tatcgttaaa
      241 agtgcagtat gcgcaatcac cacataaatg acaacactcc caataataac aaaggctaaa
      301 aagagtttca tcagtatact taaataatca tacaatgcat taaaactctc aattttgtta
      361 atgtgatcac cacttacttc aatcccgctt tcattattgc taacttcatt aacaatatgc
      421 ataaatttat ccttttgcgc tgcactttta aagtgacatt taaagcggta gttagatagt
      481 tcatgttctg gatttaaatt aagcaaataa ttattaagtg ttaaaccagg ctttaaaaat
      541 atattaccta aggttgcatc aatcgtattt tgggaaatgt ataatttagg gacagaaaaa
      601 tatgtcggtt catcgtatat ataagtaata ggaaactgat tataataatc aattaacact
      661 tcttcatcat cgataaatat tgtcgtcttt atataaaaat gtagcaacat ttcatcatcc
      721 tttattgata caagtttatt aagttcgttg taaaaggctc ggttaatgcc aatctcatta
      781 gtgttctccc caataattgt aattaagaca ttaggaattt ctttacctaa aaccataatt
      841 tctttttcat aaagtaaggg tgaataatct ggtctaataa caaaagattc tgttactttc
      901 attatcgaat taaggtaatc aatacttggt cgtcgataag atttaacatt aatcagcgga
      961 ttgttagtag gttgcgattc ataaagggca acatccacac ttaaatattc taaataacct
     1021 tccgcaagag tttgggctag tgtttcacta cttgaaaaga aaactaaaaa taacatcagc
     1081 gtgatattag taaaaatcat aaaaataacc attaatcgat ttttctttat atttcgttta
     1141 tataaattag caataacttt aaataatgtt attatcttca tgaaaaactc cgttcttaat
     1201 ccttaaaaaa atagtattac cacttttaaa taaacttggt tggtgtgttg ttactattac
     1261 gattttatta gcgcttaatt cgctaagaag cttcgctact atttgactat ttttaaaatc
     1321 aagatgggct gttggctcat caattaataa aatgtgtttt cgttcaataa taccacgcaa
     1381 tatatttgca cgcgcctttt ctccgccact acatacattt gctcttcttt gcaaaatatg
     1441 gctgatttgc agtgctttag tgtatttttg tcgtattttt cgattgttta aatggcgaaa
     1501 aagtgaacta attttaagat tatcaattat ccttaaatca cctaaaaaat atggatgctg
     1561 caaaactaaa gtaacatcat ttttagtaat cagtttattt ttatatttaa ttttcccact
     1621 gtttttagaa atataattag cgacaatatg taaaaaggtt gattttccgc ttcctgaata
     1681 accaacgatg acatatatct tcccactttt aaaagtataa gaaagattac taaatatttc
     1741 ttttttgcca aaacttttat atagattaac gacttgtaaa ataggaacct cctactttac
     1801 aaataaaaca ttatttcgta aaaattatcg tttcgtgact agcaatgtgt tttttaaact
     1861 tttgattaaa cttaatcctg ctaattaaaa cgatgtttcc acaaccctga cattgcaatt
     1921 ttaaatcagc accaagcatc accacttgaa agcgatgact cttatgaatg caaggatgcg
     1981 gcttcttcat ttcaatttca tcataaagat caactttaat taaagccact agcgaacctc
     2041 cttcatatta accgcagcag gtactttagg gagtccaggc attgttaata ctttaccagt
     2101 tagggcaacg atgaaacctg ccccagcact tagtctaaat tcgcggattg taatattgaa
     2161 gtttcgtggc gcatttttta aatttggatt atcactaaat gataacggag ttttagccat
     2221 acacaccggt aatttagcaa acccttgttc tttaattacc gcaagttcgc gttgtgctct
     2281 tttactatat ttaaccccgc ttgcgcgata taacttttta catataattt caatcttttc
     2341 ttcaacttct ttctcagtat cataaagaaa ttcgaactta gtttcttccg ctagtaatgc
     2401 ttttacttta tttgcaaatt caataccacc ttcactgcct ttactcaccg agctaacaag
     2461 tgcataatca tatcctagtt tttgcatttc tttttcaaat aacttcactt cacttttatg
     2521 atcattatca aaaagattaa gagcaacaag aactttaaca ccaaataaac ttaagttttc
     2581 ataatgctgc actaagtttt caaatccttt ggcaagtgca tcagtatttt cttcataaat
     2641 tgcactcatt ggaacgccac catgcatctt taatgcctta atagtggcaa ctacgaccgc
     2701 tagattaggc tttaaattag cggtacgaca cttaatattg aaaaactttt ccgcacctaa
     2761 ttcactagca aaacctgctt cagtgataac gactggtgct ttacttaacg catattttgt
     2821 cgcaatgatt gaattacagc catgggcaat atttgcaaaa ggtccgccat ggacaagaac
     2881 gggattgcct tctaatgttt gcaccaaatt tggtaataat gcttgtttca ttaagtgcat
     2941 aatcgcatct tctatttcta aagcggcaag acgaacaatt tcaccttcat atgtatagcc
     3001 aataataata tcttttacac gacgaataaa gtcatcagga tcactagcaa gacacataat
     3061 ggccattagt tcactagcga cagtaatttg aaattcttcg cagcgaggag taccattgcc
     3121 cgtaccttga ttaattctaa ctttgcgtaa gccacgatca ttcatatcta aagagcgttt
     3181 ccatgtgata cgtttcgggt ctaaatttaa agcattgcct tggaaaatat gattatcaat
     3241 tactgcggca attagattaa tgcttgaagt aagagcgtgg agatcacctg tgaaatgaag
     3301 attaatatcg tcacttggat aaagcttagc gcgtcctgca ccagtggcac cacctttaat
     3361 cccaaacact ggaccaagtg aaggctcccg taaacaaagt aaactctttg ttcccgtttt
     3421 ccacattgcg tcttgtagag caattgacat tgtcgttttc ccttcaccaa aaggtgttgg
     3481 tgtaatcgca gttactaaaa ttaatttacc ttgtggatta atatcagtaa acggactttt
     3541 aatctttgct ttatcttcac cataaagtat tacatctgca ggtgaaagac caactttctt
     3601 tgcaacttct aaaatgtgta atgtgttttt cataatgagt cctcatactt tcctcttaga
     3661 taattaaacg ctgcttcttt atcagtaaac catttagcgg caaactgatt acgaaaaaaa
     3721 gttcgctgtc ttttcgcata ttgtcttgta cgtaaggcaa ttaaatttat taattcttca
     3781 tcagtggcat taggattttg cacaatctct ttataaccaa ttgcctgcat tccctgtgca
     3841 tttcgcgggt atttcttgct taatgtcatc gcttcttctc ttaatccctt gtcaaacatt
     3901 aacttaactc ttgtttcaat atcgcgatgg agcatttcat tttctttatc taaacaaatc
     3961 ataatatacg gataaagtgg ctcacttgat gttacttctt caagcgcagt ttttgtttga
     4021 gcgtgagttt taattaaagc gagtgcccgc agtacgcgct tacgattatt aggatgaatc
     4081 ttagcggcag cttctttatc taacacttct aattctttgt gcagtgaagt attacttatg
     4141 ttagggtcat caacaatctt taagtccttt tccggttgta agttatagtt atataaggct
     4201 gctctaatat aaagaccgct gccaccaacg agcactatcg gaattttttt agctaaatac
     4261 ttatctaata taatccgtac ttctttttga tagttataaa tagaaaagcg ttcatctaat
     4321 gaaatattgt taaataaaaa agtcggaagc atcgctaatt cttctttggt tggtttagca
     4381 gttcctatat ttaactcttt ataaaaagca aacgcatcag cgttaataat aattccgttt
     4441 attgctttag caaagcggaa ggctaaatca cttttcccgc ttgcggtcac accaccaatg
     4501 acaaaaatca tgtgcctgtc ctcttaaaca ttttttctaa ttcgtacttc gtaaacttaa
     4561 taattgtcgg ccgaccatgc ggacaactaa acggattatc acacgctagt aattctttta
     4621 ttaaatgttg catttccata attgaaaggc ggtgattagc tttaattgat gctttacaag
     4681 ccattgttgc aatagcgtta gtgcgtaaaa gctcttgatt taaacgctta tcagtaataa
     4741 cagcatcaat gatatcttga atataaatgc gttcatcata ttcacctgcc caactaggca
     4801 cagcagtaac tcttagtgta tttgggccaa ataactctgc ttcaattccg acatctttaa
     4861 gaatggtaaa tacttcagga gtaaaaagcg ctatttcact atgactaaaa gataaaagtt
     4921 caggcactaa aagtggtcta gtttttaaat ccatatttaa tactttttga aatttctcat
     4981 aattaatccg ttcatgagca gcgtgttggt caataagcca aaagccccca tcatttactt
     5041 cgcaaataat gtaggtgtta tggagtgctg caactggatt aacaaaacta ttttctaatt
     5101 cactaattgt tttagttatt ggttttctaa ttgcttcttg atgatgacta tcactaattt
     5161 ttaattcact agtttcagtg ccaaacggca aatcaaaaat aagttgctcg gctctaggtt
     5221 cttcgttagt agtgcttgat agtggttgca ctttttggtg cggatcttta gggataaatg
     5281 cttctttacg gagcgctagc ggaatttctt ttaataaaac attgcgtaag tcttcttctt
     5341 ttgaaaaacg cacttctttt ttagtcggat gcacattaac atcgactaat tctggttcta
     5401 tttgtaaatt taaaatgaca aacggataac gcgtgggagc aataaagccg tgatacgctt
     5461 caattatcgt actttgcact ttcggcatat atacattacg accatttaag tttgtaatga
     5521 tcccgtaacg attagaacga actaaattcg tgttccctaa gaaacctgta atcgtaaaat
     5581 cattcgtttt aacactaatt ggtaataagt ttttaacata tgttaaacca taaatattta
     5641 aaattgtttc ttctaaatta tttcgcccac ttgtgcgaaa tcttaactta tcatcaaatg
     5701 ttaaactaaa actaacatca ggatgggcta aagctatttt agtcacaaca tcaagtgtta
     5761 cagcgttttc ggtataatcg cgttttaaat atttaaggcg cgctggtgta ttgtaaaaaa
     5821 gttcagagac agtaattttt gtaccactac taactgcact tggctcaatt ttaatatcac
     5881 cattttcaat cttagcaagt gctccaggtt tccccttttc agcagtttca acttgcactt
     5941 ttgcaattga agcaatactt ggtagtgctt cacctctaaa ccctaaagtt cttattctaa
     6001 ataaattgaa agttgaatga atcttacttg tagcatgtcg cgaaaaagct agcgctaatt
     6061 cgtcgctagg aatcccgcta ccattatctt gtacaattat tgctttacgt ccggcttcat
     6121 taataaaaac atcaatttta gtagcaccag cgtcaatact attttccact aattctttaa
     6181 caactgaact tggtctttca attacctccc ccgctgcaat ttgattagca agttcgttgt
     6241 ctagtaaaat gatcttagcc attatttaaa cccttcatct ttaattttct ttaaaagaat
     6301 aagggcatct aggggagtca tttcatcaat atttaaattt tcgagctgtt gataccataa
     6361 tggttttagt ggcacatatt ctgttttagt cggattgccg ccactattgt tagcgacaaa
     6421 tgtttgtaaa tgttcttggg cattacttaa aatttctagc ggtaatcccg ctaaacgggc
     6481 aacattaatc ccgtaactct tattcatagg accaggttta attttataaa ggaaggttac
     6541 attattttcg acttctttta cttcaacatg aatgttttta atattaggat aatgctcatc
     6601 aattgcagta atttcgtgat agtgcgtgct aaataatgtc tttactttta acttgttagc
     6661 aatatattca agcattgcgt aggctaaagc cattccgtca taagttgaag tgccgcgacc
     6721 aatttcatca aatagtaaaa gtgaatttgg tgttgctttg cgtaaagcat aatttgcttc
     6781 aatcatttca accataaatg ttgattgtcc gcttattaaa tcatcgcttg ccccaatccg
     6841 tgtaaagatt tgatcaaaaa tcattaagtt tgcgctcttt gccggaacaa aactgccaat
     6901 ctgcgccata atcacaatta gcgctaattg acgcataagt gtcgatttac cacccatatt
     6961 tggaccagta ataatcacga tatcggtcgc ttcatctagc tcaatatcat ttgcaacaaa
     7021 ggcgtggtct ttaagtaatt tacttaaaac agggtgccgt gcttctttaa tatcaaccac
     7081 gcgtgcatcg ttaaatatag gacgaacata attattagcg accgccactt ccgctagaga
     7141 aataaaaaca tcaatttcac ttacaacatc ggcactttgt tgtaataatc gtgttgactt
     7201 ctttaactca tttaaaagcg caccgaaaag ttctctttct aattgttgcc gtcgttcttc
     7261 agcacttaga attagtgttt cttgttcttt aagcgcttca ttaataaagc gttcaccgtt
     7321 ctttgttgtt tgtttgcgga tataaccaaa ttcatcttta acttgatcta aattgctatt
     7381 actaacttca atgtaataac caaatacacg attataacca attttcaaat ttttaattcc
     7441 tgtccgcact cgttcatttt cttcaaaagc actaatccat tttttaccac catgcgctaa
     7501 atcaattagt tcatcaagcg cttcataata tccatactta aatatttcgc caccagttat
     7561 ttgtagtggg gcgtcttcac taattgcttt ttccagtaag gtaattaaag gaatattact
     7621 tgcaagctta tcatagagtt cactaatttc aggttctcta attttaccta aaagattacg
     7681 tgctttttct agtgcttgga gtgaagtctt aaggcgcaac aagtcacgcg gattagcgct
     7741 accaaaagag atgcgcgcca ctatacgttc taagtcataa atactaacaa aatgttcttt
     7801 aacttccgca cgtgttacaa agttaacaat aaatgtgttt acaatatttt gccgccgaat
     7861 aatttcatca taatcgtgtg aaggacggat aataaaatct tttagtttac gacgacccat
     7921 cgctgttgtt gtttcatcta atagccaaaa tagagacccg tattttgttt cattacgaat
     7981 tgttcttgtt aactctaaat taaggcgtga aaaagcatct atttgtaaga aagactgcgg
     8041 ttttgtcaca gtaattgctt taaaatatga taattgtcgt tttttagttc gtgttaaata
     8101 actcactaaa cgcgcggcaa tcaaatactc ccgctcatcc ttaacatcac taaataaatc
     8161 ttcatattct tcatcaattt ttgtattctc ttcaaacgat ataacaagat ttatatttgt
     8221 tttaataaca cctaaaagct tatcactaaa ctgtggactc acaacaattt cacgagtgtt
     8281 taatccgttt aaataactta ctaaactcgt atcttcacgc ggtaaagttg tgacgaaaat
     8341 gtcaccagta gaaacatctg cgtaacttac agtaaaagca tcagcgtaag catcaaccgc
     8401 tgtgataaag ttattatcac tatcttcaaa atcgacaatc gaaccaggag ttacaatttg
     8461 gattatttct ctttttacaa tcccctttgc tgaactagga tcttccattt gttcaacaat
     8521 tccaacctta tgcccagcgt caattaactt ggcaagatac ggaccataag cgtgatgggg
     8581 tatcccgcac attgggattt tacgattatt tcctgctgct cttttagtga gcactaattg
     8641 taattctttg gatacaattt cagcatcttc aaaaaagaat tcataaaaat caccaagtcg
     8701 atacataatt aaaatacccg ggttttcccg tttaatttct aaatactgct caatcatcgg
     8761 agtatagacg atttttttct tctcttttcc catagtgtca ttttaccata catggtaact
     8821 tgattattca attacttttt ttaaagtata aagaagttct tcaacttccg catgtaatga
     8881 gttgtaattt aaaatataag gatgattttc ataagctttt tttaacacta aatattcttc
     8941 ttgatatttc ttatgtacta ctttatcgcc catattttgt gtcatttttt gttgtaattc
     9001 ttttaatttt gtttctgttt ctttt
//
LOCUS       DAOPCD010000011         8972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_7310_length_8972_cov_4.001121, whole genome shotgun sequence.
ACCESSION   DAOPCD010000011 DAOPCD010000000
VERSION     DAOPCD010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8972
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7310_length_8972_cov_4.001121"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..637
                     /gene="nth"
                     /locus_tag="PLQ55_00730"
     CDS             2..637
                     /gene="nth"
                     /locus_tag="PLQ55_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPK28497.1"
                     /translation="MTRTNKERILAFLDKEYSDAKGELEYTKDYELLIAVVLSAQSTD
                     VAVNNVTRKLFAKYPTFESLLNVKEAQFAAYFKTLGLYRNKAKHVYNLIHLLNEHHNG
                     VVPSAKEELLKLPSVGVKTANVVRAELFNIPEIAVDTHVSRIARRLGFAKFSDDVTII
                     EAKLRKVLPKERYIKTHHQMIHFGRYFCKAQSPLCKKCPLVDICKEPNKKL"
     gene            complement(632..1222)
                     /gene="recU"
                     /locus_tag="PLQ55_00735"
     CDS             complement(632..1222)
                     /gene="recU"
                     /locus_tag="PLQ55_00735"
                     /inference="COORDINATES: protein motif:HMM:NF002581.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HPK28498.1"
                     /translation="MINYPNGSKPKKQTTKVPSKRKNITKSAANRGMALEKLISAAND
                     YYLMHDIALFTKRPTPINVVKIDYAKGAKITNAYFEKQSTTDFNGVYKNRYFDFEAKS
                     TRLKTSFPLNNIYEHQITHLENVIRHGGIAFFIVEFTAFDEYYVLSAADVIDFYRNGD
                     RKSIPYASFKEKGVLVRLGLNPVLDYLPAVIKLFNL"
     gene            complement(1222..1947)
                     /locus_tag="PLQ55_00740"
     CDS             complement(1222..1947)
                     /locus_tag="PLQ55_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPK28499.1"
                     /translation="MKKLAKATRGRKQLIFLDLEGTQFSHELIAIGAIKATIKTDGTI
                     KKLYPGFKHYVLATQNVGNYVEKLTGITDDLLAREGIPFDKILLMLKKYVGTNLSKTK
                     FVTFGAHDIRILRQSLAHTPEADHTFIKTIAKNHIDLSAYINEYIKDEKNNTLSLVNL
                     CKLFGVEPVEPAHDPLNDALMLAYLYSELFKKTDILIERYGEVLLNMNNIPRPIKRML
                     NKIKAGESVNIDNLQEYLRDEIV"
     gene            2039..2389
                     /locus_tag="PLQ55_00745"
     CDS             2039..2389
                     /locus_tag="PLQ55_00745"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HPK28500.1"
                     /translation="MAEKRLLDADKILKKKFKAKSGGYDALEVDEFFDLVRNDYESML
                     EIEKELELLRLKNETQQAKIVNLEAQYIQYKKKVEELERLISKGGTAMENLRKIDKYE
                     RQLWKMGIDPSKLK"
     gene            2394..2727
                     /gene="rnpB"
                     /locus_tag="PLQ55_00750"
     ncRNA           2394..2727
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLQ55_00750"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            2752..3390
                     /gene="pgsA"
                     /locus_tag="PLQ55_00755"
     CDS             2752..3390
                     /gene="pgsA"
                     /locus_tag="PLQ55_00755"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPK28501.1"
                     /translation="MNLPNKLTVARMILVILLIAFLLIPPTWLGNEPIILFSNVYLRY
                     FIAAIVFIVAAITDYFDGYLARKFNLITNFGKFMDPIADKMLVNSMLIILTVPHQAFV
                     GQISVPVIFVVLMIARDTVVDGFRLVAANQNKVLAANIFGKAKTVLQMIAIVMFLFND
                     WPFSHQMQSDSFPIVSMIVIGAATFVSLLSGFIYIFQNISVLKETHDDTTAH"
     gene            3368..3841
                     /locus_tag="PLQ55_00760"
     CDS             3368..3841
                     /locus_tag="PLQ55_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HPK28502.1"
                     /translation="MTTQLLINELIDKNLTIGSVESFTGGLFAATLVNYPGVSKTYKG
                     TIVAYSNDVKERLLNIKKEDLKKFSAVSSVVALDLARHGQKALGVDFCFGFTGDAGPT
                     ASGQSGEVGDVYIALAFANDEIVEHFKFKGDRLKVRTDAVNAALKMLARVLKNFE"
     gene            3875..4876
                     /gene="recA"
                     /locus_tag="PLQ55_00765"
     CDS             3875..4876
                     /gene="recA"
                     /locus_tag="PLQ55_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPK28503.1"
                     /translation="MSKKKDANILQETLKEIEKSFGKGAIMKLGERPVINVDVIPSGS
                     LLIDEALGVGGYPKGRIIEIFGPESSGKTTLALHAIAECQKKGGQAAFVDAEHAIDPI
                     YAKNLGINIDELLLAQPDSGEQALEIVVRLAESGVIDLIVVDSVAALVPEAELSGEMG
                     DNQVGLQARLMSKAMRKLAGVLNKAECTVIFINQLREKVGIIYGNPEVTSGGRALKFY
                     SSIRIDIRRGEAIKQGSKLIGNQAKIRIVKNKVAPPFAACGIDIIYGKGISKIGEILE
                     LAVNRGFIKKSGSWYEHDGLKIGQGREAAQNYILERPELLADLEEKIKTGVVATETK"
     gene            5170..6720
                     /gene="rny"
                     /locus_tag="PLQ55_00770"
     CDS             5170..6720
                     /gene="rny"
                     /locus_tag="PLQ55_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004801659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPK28504.1"
                     /translation="MEVLYYALFFLVGLGLGIGLFFLIPAFKAKQAFAKAQKLTKEAE
                     IKAEHIVKNAELDAKQIIYEGKQELEQAGKERRAQIRELERLLNDREKGIERRDQLLH
                     SKEQSLDARHENLSVKIKEADELKKDLQIKIDSILGELETVAQMTVSEARDELYHRVD
                     AKLEKEIALYIKQKEEEAELQVNEQARDILANTIARYSQEVTTERTVSTVELPNDEMK
                     GRIIGREGRNIRTLEQLLGVDILIDDTPEVITVSCFDPIRRETARRTIELLVKDGRIQ
                     PSRIEEVVEKATQELDEIIIKTGKDALAELGIPNVHKDMIPFIGRLKYRTSYGQSALK
                     HSIEVAHLCGIMAAELGLDITRAKRAGLFHDIGKSADFELEGSHVEVGARLAKKFNEP
                     DYVIDAIESHHGDKESRFIFSQLVIAADTLSAARPGARTETLENYIKRLEKLEEICKS
                     YDGVQTSFAMQSGREVRVMVHPDKVDDIMVNKLVRDIRERIENEMTYPGQIKVSVIRE
                     YRASDVAK"
     gene            6723..7520
                     /locus_tag="PLQ55_00775"
     CDS             6723..7520
                     /locus_tag="PLQ55_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPK28505.1"
                     /translation="MVKILFIGDIVGKIGRETLAHFLPILKREHDFDLIIANGENTTH
                     GRGLTKKHYEELVNLGIDVITLGNHFDDRPELKYYITEVHNLVRPLNLIENYPGEGSV
                     VVTTKKGDKVRVTNVLGQAFMKEIITSPYANLKMLNADTEKMIHIVDYHAEATAEKMT
                     IGWEFDGAVSAVLGTHTHVQTRDARILPKGTAYISDVGMTGAYNGIIGADRERVIERT
                     WFDKQVPFTYEYKDKEAQFSAVILEINKHTFKTEKITPLYYLWRRDE"
     gene            7513..8949
                     /gene="miaB"
                     /locus_tag="PLQ55_00780"
     CDS             7513..8949
                     /gene="miaB"
                     /locus_tag="PLQ55_00780"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPK28506.1"
                     /translation="MSKTLLNNLPSFTKARRRYPSIVQNLKIEVPSIVSAKLSGKFFL
                     IKTFGCQANVRDEESMAGLLSMANMRRTEEEKDASVIIINTCAVREGAEQKIFGQVGN
                     LKGLKTNNKDLIVIVAGCVVQQEESLAKLRKTFPYVDIFMGTNAIHKLLDLIALRITE
                     NKKVVDVPSDDADVYENIPVQRIAPHKAFVNITYGCDKFCTYCIVPYTRGKERSRLPA
                     DILRECQILVDSGYQEITLVGQNVNAYGIDLKDGTTFSYILEEVAKLGIPRLRFITSH
                     PWNFDDEMIDIIAKYPNIMRKIHLPLQSGSDEILKLMGRRYTVKDYLELVTKLRARMP
                     DISLTTDIIVGFPNETEQQFLETLKVVEKVNYENAYTFIYSPRKGTPAALMEDNVSRE
                     EKGERFNKLKALVDSQTEANAALYVGNEYLILVDGYSKKDTSVLSGYTESDRVVHFVG
                     TPDLIGKIVKVKITESRVYSFVGELVNE"
BASE COUNT         3024 a         1422 c         1795 g         2731 t
ORIGIN      
        1 gatgacgaga acgaataaag aaagaatact cgcctttctt gataaagaat atagtgacgc
       61 taaaggtgag ctagaataca caaaagatta tgaactgctt attgctgttg ttttaagcgc
      121 acaatcaact gatgtagcgg ttaataatgt tacgcgaaaa ttatttgcaa agtatccaac
      181 ttttgaaagc ttattaaatg taaaagaagc acaatttgcc gcgtatttta aaacacttgg
      241 cttatatcgc aacaaagcaa aacatgtgta taatttaatt catttactta atgaacatca
      301 taacggagtt gttcctagtg ctaaagaaga gcttttaaaa ttacctagtg ttggtgttaa
      361 aaccgcgaat gttgtacgcg ctgaactctt taacattccc gaaattgccg tggacactca
      421 tgtttcacga atagcgcgtc gccttggttt tgctaagttt agtgatgatg taactatcat
      481 cgaagcgaag ttacgtaaag ttttacctaa agaaagatat ataaaaacgc atcaccaaat
      541 gattcatttt ggtcggtatt tttgtaaagc acaaagtccg ctatgtaaaa aatgtccgct
      601 tgtggacatt tgtaaagaac caaataaaaa gttataaatt aaataatttt atgactgcag
      661 gcaaatagtc aagtacaggg ttaagaccaa ggcgcactaa tacgcctttt tctttaaatg
      721 atgcataagg gattgattta cggtcgccat tgcgataaaa atcaataaca tcagcagcgc
      781 taagtacata atattcatca aaagctgtaa attcaacaat aaagaaagca atcccaccat
      841 ggcgaataac attttctaag tgggtaattt gatgttcata aatattattt aaaggaaaac
      901 ttgtttttaa tcttgtactt ttagcttcaa agtcgaaata tcggttttta taaaccccgt
      961 taaagtctgt tgttgattgc ttttcaaagt aggcgtttgt aatttttgcc cctttagcgt
     1021 aatcaatttt tacgacatta attggtgttg ggcgtttagt aaaaagggca atgtcgtgca
     1081 tcaaataata gtcattagca gcactgataa gtttttcaag cgccatgcca cgattagcag
     1141 cgctcttcgt aatatttttt cgttttgaag gtactttcgt tgtttgtttt ttaggtttag
     1201 aaccgtttgg ataattaatc attacacaat ttcatcgcgt aaatattctt gtaaattatc
     1261 aatatttaca ctttctcctg cctttatttt attgagcatt ctttttattg gacgcgggat
     1321 attattcata ttgagtagta cctcaccata ccgttcaata agaatatcag tctttttaaa
     1381 gagctcacta tataaatagg cgagcatcag ggcatcattt aacgggtcat gagcaggttc
     1441 aactggctca acgccgaaaa gtttacataa attcaccaaa gataatgtat tatttttttc
     1501 atcttttata tattcattaa tgtatgcact taaatcaata tgatttttcg caatcgtttt
     1561 aataaaagta tgatcagctt caggagtatg ggcaagtgat tggcgtaaga tacgaatatc
     1621 gtgtgcccca aatgtcacaa atttagtttt tgaaagatta gtgccaacat attttttaag
     1681 catcagtaga atcttatcaa aaggaatgcc ttctcttgct aataaatcat ccgtaatgcc
     1741 agttaatttt tcaacataat taccaacatt ttgtgttgct agcacataat gtttaaagcc
     1801 aggataaagt tttttaatcg ttccgtccgt tttaattgtg gctttaattg ccccaattgc
     1861 tattagttca tgactaaatt gtgtaccttc taaatcaagg aagataagtt gcttgcggcc
     1921 acgtgttgct ttcgctaatt ttttcatact ataaaattat aacatagtaa taataaagcg
     1981 gtctcacttt gttgcgtcaa aacaaaatta catatataat atgtatgcga ggttaaggat
     2041 ggcagaaaag agattactag acgcagacaa aattttaaaa aagaagttca aagcgaaaag
     2101 cggtggctat gacgctttag aagttgacga attttttgat ttagtgcgta atgactatga
     2161 atctatgctc gaaattgaaa aagaattaga attactgcgg ctgaaaaatg aaactcaaca
     2221 agctaagatt gttaatcttg aagcgcaata cattcaatat aaaaagaaag ttgaagagct
     2281 agaacgctta attagtaaag gcggaaccgc gatggaaaac ttacgtaaaa ttgataagta
     2341 tgagcgtcaa ttatggaaaa tgggcattga cccaagcaaa ctcaaataaa attcgatctg
     2401 gataattgct ggtacataag tattagagga aagtccatgc tcgcacttgc tgcgatgcaa
     2461 gtagtgattg cgctagtgga aaaaataaca ctaggtatag aggagtctat aacggcgaag
     2521 aaagacctaa gtcgaaagat atggttaatc ttctgaaggt gccacagtga cgaagttaag
     2581 gggaaactct taagtggaac gttggtaaac cccgcaagcg agaaacctaa atttggtaag
     2641 ggaaacggca atagagaaat gaatttagcg ccgtatgtgt ttacacatag ataaataatt
     2701 atccgcgaca gaacatggct tacaggatcg aaactcacct aaaggagcat tatgaattta
     2761 ccgaataaat taacagtagc gagaatgata cttgtcatcc ttttaattgc ttttttactt
     2821 attccgccca cttggttagg taatgaacca attattcttt tttctaatgt ttatctgcgt
     2881 tattttattg ccgcaattgt ttttatagta gcggcaatca ctgattactt cgatggatac
     2941 ttagcgagaa aatttaattt aattacaaat tttgggaagt ttatggaccc aattgccgac
     3001 aaaatgctgg tgaattctat gttaatcatc cttacagttc cgcatcaagc gttcgtcggg
     3061 caaataagtg tacctgttat ttttgttgta ctcatgattg cgcgtgatac agtagtggac
     3121 gggtttagac ttgttgctgc aaatcaaaat aaagtactag cggcgaatat ttttggtaag
     3181 gctaaaacag ttttacaaat gattgcaatt gtgatgtttt tatttaatga ttggcccttt
     3241 tcacaccaaa tgcaaagtga ttcgtttccg attgttagca tgatagtaat tggcgctgct
     3301 acatttgtgt cattattatc aggctttatt tatatattcc aaaatatcag tgtacttaag
     3361 gagacgcatg acgacacaac tgctcattaa tgaattaatt gataaaaatt taacaattgg
     3421 tagtgttgag tcttttactg gtggtttatt tgcggcaaca ctcgtgaatt atccaggtgt
     3481 tagtaaaaca tataaaggga cgattgtggc ttatagtaac gatgtcaaag agcgtttact
     3541 taacattaaa aaagaagatc taaaaaaatt tagtgccgtt tcaagtgttg tagcgcttga
     3601 tttagcgcgt catggccaaa aagcattagg agttgatttt tgttttggct ttacgggtga
     3661 cgcaggtcca acagcatcag gccaaagcgg agaagttggt gatgtttata ttgccctagc
     3721 gtttgctaac gacgaaattg ttgaacactt caaatttaaa ggtgatcggc ttaaagtccg
     3781 cactgatgca gtgaatgctg ccttaaaaat gttagcgaga gtgttaaaaa acttcgaata
     3841 atattgtata tatctatata aggagaaaat tattatgtca aaaaagaaag atgcaaatat
     3901 tttacaagaa acattaaaag aaattgaaaa gtcgtttggt aaaggcgcca ttatgaaact
     3961 aggcgaacgc ccagttatta atgttgatgt cattccgagc ggctcattat taattgatga
     4021 agcacttggc gttggtggct atccaaaagg gcgtattatt gaaatctttg gaccagaaag
     4081 tagtgggaaa actactttag cgctgcacgc aattgctgaa tgtcaaaaga aaggtggtca
     4141 agctgctttt gtcgatgctg aacatgcaat tgacccgatt tatgcgaaaa acttaggtat
     4201 taatattgat gaattacttt tagcacaacc tgattcaggt gaacaagcac tggaaattgt
     4261 tgtccgtctt gccgaaagtg gtgtgattga tttaatcgtt gttgatagtg tggctgcact
     4321 tgtacctgaa gcagaactat ctggcgaaat gggtgataac caagtaggac tacaagcacg
     4381 cctgatgtca aaagcaatgc gtaaactagc gggtgtatta aataaagctg agtgtactgt
     4441 tatctttatt aatcaactac gcgaaaaggt tggaataatt tatggcaatc ctgaagttac
     4501 ttctggtggt cgtgcattaa aattttattc ttccattcgt attgacatta gacgcggtga
     4561 agcgattaag caaggttcta aattaattgg taatcaagca aaaattcgta ttgtaaaaaa
     4621 caaagttgca ccaccatttg ccgcttgtgg aatcgatata atttatggca aaggtatttc
     4681 taaaattggg gaaattcttg aattagcggt taaccgcgga tttattaaaa agtcgggttc
     4741 gtggtatgaa cacgatggac ttaagattgg acaaggccgc gaagcagcgc aaaattatat
     4801 tttggagcgc ccagaattac tcgctgattt agaagaaaaa attaaaacag gcgtagtcgc
     4861 cactgaaact aaataattac aattatttta taaattttaa aattagaaaa cacaattttt
     4921 aaaattgtgt actttactaa tcatctttgc tttaattaat gctaaaaagt tgttataatc
     4981 aaagtgcgca ctaagtgcgt tcacctaaac ttcatgttag gttattaatt cactttattg
     5041 aaatttattt acataaatca tcgctaaaca ttaatgatgt aacattggaa tttaggtaac
     5101 agactatttt tcgtttgtat atattttcaa acacaaaatt ttaaaaaaca aagaaagaag
     5161 gaacacaaaa tggaagtttt gtactatgca ctattctttc ttgtcggact tggacttgga
     5221 atcggactat ttttcctcat tccagcattt aaagcgaagc aagcatttgc gaaagcacaa
     5281 aaattaacaa aagaagcgga aattaaagcc gaacacattg taaagaatgc tgaacttgac
     5341 gctaaacaaa tcatttatga aggaaaacaa gaactcgaac aagccggcaa agaacggcgt
     5401 gcccaaattc gtgagctaga aaggctcctt aatgatcgtg aaaaaggaat tgaacgtcgc
     5461 gatcaattat tacatagcaa agagcagtca cttgacgctc gtcatgaaaa cttatcagtg
     5521 aaaatcaaag aagctgatga actcaaaaaa gacctacaaa ttaagattga ctcaatttta
     5581 ggcgagcttg aaacagtcgc acaaatgaca gttagtgagg cgcgagatga attatatcat
     5641 cgcgttgacg ctaaactgga aaaagaaatc gcgctgtata ttaagcaaaa agaagaagaa
     5701 gccgaactac aagttaatga acaagctcgt gatattttag caaacacgat tgcgcgttat
     5761 tcacaagaag tgacgactga acgcactgtt agtacggtcg aattaccaaa tgatgaaatg
     5821 aaaggacgca ttatcggtcg tgaaggacgc aacatccgca cacttgaaca actattaggc
     5881 gttgatattt taattgatga tacaccagaa gttattactg tgtcatgctt tgatccaatt
     5941 cgccgtgaga ccgctagacg gacgattgaa ctattagtca aagacggccg aattcaacca
     6001 agtcgtattg aagaagttgt ggaaaaagca acgcaagaac ttgatgaaat tattattaag
     6061 actggtaaag acgctcttgc cgaacttggg attcctaatg tccacaaaga tatgattcca
     6121 tttattggcc gcttaaaata ccgcacgagt tacggacaaa gtgcacttaa acatagtatt
     6181 gaagtcgccc atttatgtgg cattatggct gctgaattag ggcttgatat tacgcgcgct
     6241 aaacgtgcag gattattcca tgacattggt aaatcagcgg attttgaact tgaaggaagt
     6301 cacgttgaag ttggagcaag actagcgaaa aaattcaatg aaccagatta tgttattgat
     6361 gcaattgaaa gccaccacgg tgataaagaa tcgcgtttta tctttagtca attagtcatc
     6421 gctgctgata cattaagtgc agcgcgacca ggtgctcgga ctgaaacgct tgaaaattac
     6481 attaaacgtt tagaaaaact tgaagaaatt tgtaaatcgt atgacggtgt gcaaactagc
     6541 tttgcaatgc aatcaggccg tgaagtacgc gtgatggtcc atcctgataa ggtggatgat
     6601 attatggtca ataaactcgt tcgtgatatt cgtgaacgga ttgaaaatga aatgacttat
     6661 cctggtcaaa ttaaagtgtc ggttattcgt gaataccgtg caagtgatgt agcaaaatag
     6721 taatggtgaa aattttattt attggtgata ttgttggcaa aatcgggcga gaaactctcg
     6781 cccatttcct gcctatactt aagcgtgaac atgactttga tttaattatc gctaacggcg
     6841 aaaatacaac gcatgggcgc ggattaacga aaaaacatta tgaagaatta gtaaatcttg
     6901 ggattgatgt catcacactt ggtaatcatt ttgatgatcg tccagaactt aaatattaca
     6961 ttaccgaagt gcataattta gtgagaccat taaacttaat tgaaaattat cctggtgaag
     7021 gatctgtcgt tgtcacaacg aaaaaaggtg acaaagttcg cgtcactaat gtattaggac
     7081 aagcatttat gaaagaaatc attactagtc cgtacgctaa tcttaaaatg cttaacgctg
     7141 atacagaaaa gatgattcat atcgttgatt atcatgcaga agctactgca gaaaaaatga
     7201 cgattggctg ggaatttgat ggtgcagtaa gtgctgtttt aggtacacac actcatgtac
     7261 aaacgcgtga cgcccgtatt ttgccaaaag gtacagctta tattagtgat gttggtatga
     7321 cgggggcata taatggcatc attggtgcag atagagaacg cgtaatcgag cggacttggt
     7381 ttgataaaca agtaccgttt acttatgagt acaaagataa agaagcgcaa tttagcgctg
     7441 ttattttaga aattaataaa catacattta aaacagaaaa aataacacca ctttattact
     7501 tatggagaag agatgagtaa aacattactt aataatttac cctccttcac taaagcacga
     7561 agacgttatc cgtccatagt tcaaaatctt aagattgaag taccgtcaat tgttagtgcg
     7621 aagttaagtg gtaaattttt cttaattaaa acatttggct gtcaggcaaa tgtacgcgac
     7681 gaagaaagta tggccggtct tttaagtatg gctaatatgc gacgcactga agaagaaaaa
     7741 gacgctagtg taattattat taatacatgt gctgttcgtg aaggggctga acaaaaaatc
     7801 tttggtcaag tcggtaactt aaaaggacta aaaacaaata ataaagattt aatagtaatt
     7861 gttgcgggtt gtgttgtgca acaagaagaa tcactcgcga aattacgaaa aacattccca
     7921 tatgttgata tttttatggg gacaaacgct attcataaat tacttgattt aattgcctta
     7981 cgaataacgg aaaataaaaa agtagtggat gtgcctagtg atgatgccga tgtctatgaa
     8041 aacatccctg tgcaacgcat tgccccgcat aaagcttttg tcaatataac atatggttgt
     8101 gataagtttt gtacatattg tatcgtccct tacactaggg ggaaagaacg ctcacgatta
     8161 cctgctgata ttttacgaga atgtcaaatt ttagtggatt ctggttatca agaaattaca
     8221 ctcgttggtc aaaatgttaa tgcttatggt attgatttaa aagatggcac aacttttagt
     8281 tatattttag aagaagtagc aaaacttggc atcccacgcc ttcgttttat tacttcgcac
     8341 ccatggaact ttgatgatga aatgattgat attatcgcta aatatcctaa tatcatgcgt
     8401 aaaattcatt taccgttaca aagtggtagt gatgaaattt taaagttaat gggacgccgc
     8461 tatacggtta aagattattt agagttagtt actaaattac gggctagaat gcctgatatt
     8521 tcactgacta ctgatattat tgttggcttt ccaaatgaaa cagagcaaca atttttagaa
     8581 acattaaaag ttgttgaaaa ggtaaattac gaaaatgctt atacttttat ttattcaccg
     8641 cgcaagggta ctcccgctgc ccttatggaa gataatgttt cgcgtgaaga aaaaggtgag
     8701 cgctttaaca agttaaaagc actcgttgat agccaaaccg aagctaatgc agcgctatat
     8761 gtcgggaatg agtatcttat tttagttgat ggttacagta aaaaagatac gagtgtatta
     8821 agtggataca ccgaaagtga tcgtgttgtt cattttgtag gtactcctga tttaatcggt
     8881 aaaattgtta aagttaaaat tactgagtca cgcgtttatt catttgttgg agaactagtg
     8941 aatgaataaa gttgaaatag ccttaaatgc at
//
LOCUS       DAOPCD010000012         8886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_7392_length_8886_cov_3.813498, whole genome shotgun sequence.
ACCESSION   DAOPCD010000012 DAOPCD010000000
VERSION     DAOPCD010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8886
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7392_length_8886_cov_3.813498"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1064)
                     /gene="rpsB"
                     /locus_tag="PLQ55_00785"
     CDS             complement(15..1064)
                     /gene="rpsB"
                     /locus_tag="PLQ55_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004799479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPK28507.1"
                     /translation="MSEEEKVVLTEEEVTPAMENVLPDENLPVITMKKLLEAGAHFGH
                     QTKFWNPKMKPYIYGSRNNVHIIDLEKTIVKIEEAYAALREIVTRGGKVLFVGTRKQH
                     AEHVKSEAERSGSFYMNNRWLGGTLTNFRTISTRVKYLRDLEMQEAEGAFDRLPKKEA
                     ILIRKEMKRLNDNFEGIKEMRKVPEAVFVVSTIDEANAIREARNLGIPVFALVDTNCN
                     PDVVDYIIPSNDDATKTVQLMLTLFADAIVEAKGGIPVLAYTRDLEPETETLEEALKH
                     NEPRPRRGDGEERRPVRKTRTQHSDKPTRARKAEVKEDEEVVEEIAEEKPAPKAKVKK
                     EEIVKPEEPKEEKAP"
     gene            complement(1236..2150)
                     /locus_tag="PLQ55_00790"
     CDS             complement(1236..2150)
                     /locus_tag="PLQ55_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPK28508.1"
                     /translation="MNRPLQEFQDYLRYTRRFSENTVIAYISDIETFYLFLANQNMRD
                     DEVDKPLIRLYLKDQIDKYVSRRSIQRRLASLRHYYSFLQDKRYVKDNPFLTVQAPKA
                     TKALPRVLYEEEIRLILLANEKRTDYLRDRDQLIIELMFATGLRASEVINLTLQSFNL
                     NERFLMVLGKGKKERMVPFTDRVQVLLRNYLNGLRKELLAKRKSSQPTNILFLNAQGN
                     KLTIQGLRYILLSIEKKTGEYVNLHPHLLRHSFATYLLEKGANLRVIQELLGHESINT
                     TQIYTHVSEEKITKEYYTAHPRAKKKID"
     gene            complement(2143..3435)
                     /gene="trmFO"
                     /locus_tag="PLQ55_00795"
     CDS             complement(2143..3435)
                     /gene="trmFO"
                     /locus_tag="PLQ55_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HPK28509.1"
                     /translation="MEQVVIIGGGLAGVEAAFFLAEKGIKVKLYEMRPGVMTPAHHSA
                     KLGELVCSNSLKSKELTNACGLLKKEMEIYNSLMTSVAARVSVPGGNALCVDRELYSE
                     EVTRIITTHPLIKVVNEEVVELPSHVPVIIASGPLTSKGLENTISKLIDTAFLHFFDA
                     SAPIIEKDSINMDIAYYKSRYDQGDDAYINCPFTKEEYYRFYDELINAKLAPIHEMDT
                     KYFDACMPLEVIAKRGPKTLRFGPLKPRGLGKDEHHKPYAVLQLRQDNVIGSLYNLVG
                     FQTNLTYAEQRRVFRLIPGLENAEFVRYGLMHRNTFVNAPRVLNLDKSLKVNNDIYIA
                     GQLSGVEGYVESAASGLYVALNVYAKIKGVKLEYPCETMLGSLMHYLNNANPENFAPM
                     NANFGIVRGATKQNRMEIAEKSLKITQKYKEYIESLNE"
     gene            complement(3481..5436)
                     /gene="topA"
                     /locus_tag="PLQ55_00800"
     CDS             complement(3481..5436)
                     /gene="topA"
                     /locus_tag="PLQ55_00800"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPK28510.1"
                     /translation="MKLVIVESPSKIKTIERYLSDEYKVLASYGHIVDLSTRGRKNLG
                     INLETFEGDYVLDKSRQKVADTLKKAKNESSEVYLATDPDREGEAIAVHIAKLLNLDL
                     NTTKRLEFHEITRDGITNALAAPRLLNEDLFNSQEARRMLDRIVGFELSSLLNQKINV
                     KSAGRVQSAVLKLIVDKENEITAFVPEEYWTLKIDTKYDDALFSVNFNKSYAGDTKIV
                     NEAMKNDIVSKLAPKIVVTDVKSEIKHTNPKPPFTTSTLQQEASSKFKYSASRTSRII
                     QKLYEGIQINGIETGLVTYIRTDSTRLAPSFVANASQRIVKEYGAEYLGRTQARGKTL
                     QNVQDAHEAIRPTNLNFTPDSIKDSLTREEYNLYSLIYTRALASLMVGSKEQVTRYTF
                     NSNGTIFNATSTILIFPGFRKIYGKDEETVDDSALPQMHVGSELPIIDVITEQKFTEP
                     PARYSEGRLIKTMEELGIGRPSTYAPTIQILQKRKYVSSLKGTLKPTDIGFRQIDALS
                     EFFPSIISSDYTASMENELDKIANGEGDKVEFLSDFYSDFSQKIDDARKGMKKLPPEK
                     VGRDCPRCGSNLVYREGPYGRFIGCETFPKCKYTEFIVEYTGENCPKCNEPLTYRYDS
                     RNRKYIRCSNYKSCDYSATIKSTRRKK"
     gene            complement(5545..6327)
                     /gene="dprA"
                     /locus_tag="PLQ55_00805"
     CDS             complement(5545..6327)
                     /gene="dprA"
                     /locus_tag="PLQ55_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPK28511.1"
                     /translation="MNKKARDVLLYFASKYEGNYHAIINALRSKEPVDDALFNKYKED
                     WEKVDTITIIDEDYPALFKTVNNPPIVLFLKGNKELLFNLEKSIAVIGAREYSEYGKN
                     MTYEIVEGLVKEKFTIVSGLARGIDGFSHDACLRAGGNTIAVLGSGINYPYPKSNKKL
                     YDEVSKHGLLITEYPNDTKPEPDYFKFRNRLVAALTKAVLIIEAKYRSGTIITVGYAL
                     EKGSDVFCVPERAGRNSGCNRLIKDGAYLVESASDIIKLWPN"
     gene            complement(6377..7003)
                     /locus_tag="PLQ55_00810"
     CDS             complement(6377..7003)
                     /locus_tag="PLQ55_00810"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPK28512.1"
                     /translation="MTYFEFDNDVRKHGHLYLVGVDEAGRGPLAGPVFAAAVILPPFF
                     NDERIKDSKQLTATELLELSDLIKEKALDYQIVHVDAPVIDQVNIYQATILAMQKAVT
                     GLSHEFHFVLTDAMPLSNLEVGYEAIIQGDATSLSVAAASILAKVARDAYMNKLAKLY
                     PQYDFENNKGYPTRKHLQALDTYGPIKDVHRYSYKPVQDALNKQIKLF"
     gene            7188..8024
                     /locus_tag="PLQ55_00815"
     CDS             7188..8024
                     /locus_tag="PLQ55_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28513.1"
                     /translation="MKKRKFLLIPLLLGLLLTGCFGGDGPEDIIDGDDKSSQKTTNDV
                     AEEDILENMDYEKREGWPTQKLATFLSSERVNETVPALTNIATTYYHENNDANNKYFA
                     IVVPGANREADYKAILEGAGYTYSKLSWTSIAVNTEDTINIAYQYMQDHPVLDYAMYI
                     VISALKADSFRFDKLESDIGWPANQLQTFLSKYGITERVPSLTGGNYFAELTERNEFI
                     VCAPGVNFVDSYFGSLENEGYTVNGSNAVSTSGKVRINVTANDYPFFFFDATKIVIKP
                     VV"
     gene            complement(8095..>8886)
                     /gene="ylqF"
                     /locus_tag="PLQ55_00820"
     CDS             complement(8095..>8886)
                     /gene="ylqF"
                     /locus_tag="PLQ55_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HPK28514.1"
                     /translation="QKATRKLIETLKMVDIIVELGDARIPHSSRATYLTAIIERKPNL
                     YVLTKADLADPKRVSKHTEKLNVLAGNLNERKFVRQILAKIKEIGQPIIERDVRRGLK
                     PRPLAVMIVGIPNVGKSTLINKLAGRKAASVQNKPGHTRANQYIRVSEEIALIDTPGI
                     LSPNYDEEDTSMKLALIGTIRQEILPTHTMAEYLVNYLKTYYFEEMVAFYDIDIKEDS
                     NYQTVISAIATRRGIIGEDFDVSDRTEILILNEFKNGLIGRISLE"
BASE COUNT         2581 a         1713 c         1542 g         3050 t
ORIGIN      
        1 ttttcttctt ttacctatgg tgctttttct tcttttggct cttcgggttt aactatttct
       61 tcttttttaa ccttggcttt gggcgctggt ttttcttcgg ctatttcttc aacgacttct
      121 tcgtcttctt taacttccgc tttgcgagct cgggttggtt tatcactatg ttgggtacgc
      181 gttttacgta ctggacgacg ttcttcacca tcaccacgac gtggccgtgg ctcattatgt
      241 tttaaggctt cttctaaggt ttctgtttca ggttctaagt cacgagtata agctaaaacc
      301 gggataccac cttttgcttc aacaatagca tcagcgaata aagtaagcat taattgcacg
      361 gttttagtag cgtcatcatt acttggaata atgtaatcaa cgacatctgg attacagttt
      421 gtatcgacaa gtgcgaacac tggaatacct aagttacgtg cttctctaat tgcgtttgct
      481 tcgtcaatcg tgctaacgac aaacactgct tctggaactt tgcgcatttc tttaatgcct
      541 tcaaagttat catttaagcg tttcatttct ttacgaatta agattgcttc tttcttcggt
      601 agacgatcaa aagcaccttc agcttcttgc atttctaaat cacgtaaata ttttacgcga
      661 gttgaaattg ttctaaagtt tgttaatgta ccacctaacc aacgattatt catatagaat
      721 gaaccacttc gttccgcttc acttttaacg tgttctgcat gttgtttacg ggtgccgacg
      781 aataagactt taccaccgcg agtaacaatt tcgcgtaatg cagcgtaagc ttcttcgatt
      841 ttgacaattg ttttttctaa gtcaatgata tgcacattgt tgcgtgaacc ataaatatat
      901 ggtttcattt ttgggttcca gaatttggtt tggtgaccaa aatgtgcccc agcttcaagt
      961 aattttttca tggtaatgac tggtagattt tcatctggta agacattttc cattgcggga
     1021 gttacttcct cctcagttaa tacgactttt tcttcttcac tcatgtttga gcctcctttt
     1081 gttatttcct ccacattttc gaaccttgtc cctacacctg tagaaccgga cgaggaattc
     1141 aatgtgtgcg tattagccct tttttgggcc attagtatat taccataggt aaaaggttta
     1201 ggcaactagt taacttataa agttaaatga agtgattagt ctattttctt tttagcgcgc
     1261 ggatgggcag tatagtactc ttttgtaatt ttttcttcgc taacatgcgt ataaatttgt
     1321 gttgtgttta ttgactcatg ccctaataat tcttggatga cccgtaaatt agcgcctttt
     1381 tctaataaat aagtcgcaaa agagtggcgg agtaagtgcg gatgtaagtt aacatactcc
     1441 cccgtcttct tttcaatcga aagcaatata tagcgtaacc cttggattgt taatttatta
     1501 ccttgggcat taagaaataa aatgtttgtc ggttgactac tttttctttt cgctaaaagt
     1561 tctttgcgta aaccatttaa ataattgcgt aataagactt gtactcggtc tgtaaaagga
     1621 accattcgtt ctttcttacc tttgccaagt accattaaaa agcgttcatt taagttaaaa
     1681 gattgaagtg ttaaattaat aacttcacta gcacgcaacc ctgttgcaaa cattaattca
     1741 ataattaatt gatcacgatc ccttaaataa tcagtgcgtt tttcattagc gagtaagatt
     1801 aagcgaatct cttcttcata aagcacgcga ggcaatgctt ttgttgcttt tggtgcttgt
     1861 actgttaaaa agggattatc tttcacatag cgcttatctt gtaaaaatga atagtaatgg
     1921 cgtaagcttg ctagtcggcg ctggatgctg cgccgcgata catatttatc aatttgatct
     1981 tttaaatata accggattaa tggtttatca acctcgtcgt cacgcatatt ctgattagcg
     2041 agaaataaat aaaaagtttc aatgtcactt atataagcaa tgactgtgtt ttctgaaaag
     2101 cgtctagtgt aacgtaagta atcttgaaac tcttgtaaag gtctattcat ttaatgactc
     2161 aatgtactcc ttatattttt gcgttatttt taatgatttc tcggcaattt ccatgcggtt
     2221 ttgtttagta gcacctcgaa ctattccaaa attagcgttc attggagcaa agttttctgg
     2281 attggcgtta tttaagtaat gcattaaact accaagcatt gtctcacacg gatactctaa
     2341 ttttacacct tttattttag cgtaaacatt aagggcaaca taaagcccgc tagcggcact
     2401 ttcaacataa ccttcaacgc cactaagttg accagcaata taaatatcat tgttaacttt
     2461 aagtgactta tctaaattta ggacacgcgg tgcattaaca aatgtattgc ggtgcattaa
     2521 tccgtaacga acaaactcag cgttttctaa accgggtata agtctaaata cacggcgctg
     2581 ttcagcataa gttaagtttg tttgaaagcc gactaagtta taaagcgagc caattacatt
     2641 atcttgccga agttgaagga cagcataagg tttatgatgc tcatctttac caagtccgcg
     2701 tggtttaagt ggtccaaagc gtaaggtttt aggtcctctc ttcgctatta cttctaacgg
     2761 catacaggca tcaaagtatt tcgtatccat ttcatgaatt ggcgctagtt tagcgttaat
     2821 taattcatca taaaatcgat aatattcttc ttttgtaaat ggacaattaa tataagcatc
     2881 gtccccttga tcatagcgcg atttataata agcgatatcc atattaattg aatctttttc
     2941 aatgattggt gcactagcgt caaaaaaatg tagaaacgct gtatcgataa gtttactaat
     3001 cgtgttctct aagcctttac tagttaaagg tccgctggca ataataacag gaacatgaga
     3061 aggaagttcg actacttctt cgttcactac tttaataagc ggatgagtag taataatcct
     3121 tgttacttct tcgctataaa gttcacgatc aacgcaaaga gcattaccgc cagggacact
     3181 aacacgcgca gcaacacttg tcattagtga attatatatt tccatttctt tcttaagtaa
     3241 accgcatgcg tttgtaagtt ctttactctt taatgaatta gaacacacaa gttcaccaag
     3301 cttcgcgcta tgatgagcag gcgtcatgac accaggacgc atttcatata acttaacttt
     3361 aatccctttt tccgcaagaa aaaaagccgc ttctactcct gctaatccac caccaataat
     3421 tactacttgt tccacaatga gattatagca agaaaacggc aatttcccta caaaaataag
     3481 ttatttttta cgtctagtac ttttaatagt tgcggaataa tcacaacttt tataatttga
     3541 gcaacgaata tatttacgat tacgtgaatc ataacgataa gttaaaggct cattacattt
     3601 tggacaattt tcaccagtgt attcaacaat gaattcggtg tatttacatt tcgggaaagt
     3661 ttcgcagcca ataaagcgac catatggtcc ttcgcggtac actaagtttg agccacacct
     3721 tggacaatcg cgaccgacct tttctggcgg tagtttcttc attcctttgc gcgcatcatc
     3781 aattttttga ctaaagtcac tgtagaaatc actaagaaat tcaactttat cgccttcgcc
     3841 atttgctatt ttatctaatt cattttccat tgaagcagtg taatcactac taataatact
     3901 tgggaaaaat tcgctaagag catcaatttg tctaaagcca atatccgttg gcttaagcgt
     3961 acctttaagg ctagatacat atttacgttt ttgtaatatt tgaatcgttg gtgcataagt
     4021 tgatggtcga ccaataccga gttcttccat cgttttaatt aagcggcctt cactgtaacg
     4081 cgcaggtggt tcagtaaatt tttgttcagt tataacatca ataattggta attcactacc
     4141 aacatgcatt tgcggaagcg ctgagtcatc tactgtttct tcatctttcc cataaatttt
     4201 tctaaagccc gggaatatta agattgttga tgtcgcattg aaaatagtac catttgaatt
     4261 aaatgtataa cgcgtaactt gttctttact accgaccatt aaacttgcaa gtgctcgtgt
     4321 gtaaataagt gaatacaaat tatattcttc tctcgttaat gaatctttaa ttgaatcagg
     4381 agtaaagttt aagttcgtgg gacgaatcgc ttcgtgagca tcttggacat tttgtagtgt
     4441 tttaccacgc gcttgagtac gaccaagata ttcggcccca tattctttaa cgatgcgttg
     4501 tgatgcatta gcgacaaagc ttggcgcgag tcttgtcgag tcagtacgaa tataagttac
     4561 taaccccgtt tcaatcccgt taatttgtat accttcatag agtttttgga taatccgtga
     4621 agtgcgggaa gcactatatt taaatttact acttgcttct tgttgcaagg ttgatgtggt
     4681 aaaaggcggc ttaggattag tgtgtttaat ttcactttta acatcagtca caacaatttt
     4741 aggcgctagt ttactaacga tatcattttt catcgcttcg ttaacaatct tagtatcacc
     4801 agcgtaactc ttattaaaat tcacactaaa aagagcatcg tcatatttag tgtcaatttt
     4861 tagtgtccaa tattcttcag ggacaaaagc agtgatttcg ttttccttat caacgattag
     4921 ttttaaaacc gcactttgaa ctcgcccagc gcttttaaca tttatttttt gatttaagag
     4981 actagacagt tcaaagccaa caatacggtc aagcattctt ctagcttctt ggctattaaa
     5041 aaggtcttca ttaagcaagc gtggcgcagc gagggcatta gtaatcccgt cccgcgtaat
     5101 ctcatgaaac tcaagacgct tagtcgtatt taaatcaagg tttaaaagtt tagcgatgtg
     5161 caccgcaatt gcttcacctt cacgatcagg gtcagtggca aggtagactt cactactttc
     5221 atttttagct ttttttagcg tatctgcaac tttttgccga ctcttatcta atacatagtc
     5281 accttcaaaa gtttcaagat taatacctaa attcttgcga ccacgtgttg aaaggtcgac
     5341 gatatgacca tagctagcga gtactttgta ctcgtcactt aagtagcgtt cgatcgtttt
     5401 aattttgctt ggggattcaa caattactag tttcacaaag cctcctatcg gttataaaat
     5461 atagagggtt tttttgcgct tgtcaaatgt aaaactttgt gaattttctc tttatttata
     5521 ataaataaaa cattttttat tttcttagtt tggccagagt tttataatgt cacttgcgct
     5581 ctcgacaaga taagcaccat cttttatcag tctattacag ccactattac ggccagcgcg
     5641 ctcaggaaca cagaaaacat cactcccttt ttctaaggcg taaccaaccg ttattattgt
     5701 gcctgagcga tatttagctt cgatgattaa aacggctttt gtgagcgccg ctacaagtct
     5761 attgcgaaat ttaaagtaat ctggttctgg ctttgtatca ttaggatact cagttattaa
     5821 aagaccgtgc ttactaactt cgtcatatag ttttttattt gatttaggat aaggataatt
     5881 aattccgctg cctaggaccg ctattgtgtt gccacctgcc cgtaaacacg catcatgact
     5941 aaacccgtct ataccgcgcg ctaagccact aacaatcgta aatttttctt taacgagtcc
     6001 ttcgacaatt tcataagtca tgtttttacc gtattcacta tactctcgag caccaatcac
     6061 agcgatagat ttttccaaat taaaaagtaa ttctttgtta ccttttaaaa ataaaacaat
     6121 tggcggatta ttaactgttt taaaaagcgc tgggtagtct tcatcaataa tcgttatggt
     6181 atcaactttt tcccagtctt ctttatattt attaaataaa gcgtcatcaa ctggttcttt
     6241 ggagcggagt gcattaataa ttgcgtggta attgccttca tatttactgg caaagtaaag
     6301 taaaacatcg cgtgcttttt tattcatttg tagttcctca ttatataaat aaggaagaac
     6361 ttaatttttt atcacattaa aataatttaa tttgtttatt gagcgcatct tgcactggtt
     6421 tataagagta gcgatgcaca tctttaattg ggccataagt atcaagtgct tgtaaatgtt
     6481 tccgtgttgg ataaccttta ttattttcaa aatcatattg cggatagagt ttcgcgagtt
     6541 tattcatata agcatcacgc gctactttag caagaatact tgcggcggcc acactaagcg
     6601 aagtcgcatc tccttgaata attgcttcgt accccacttc taaattagat agcggcatcg
     6661 catcagttaa cacaaaatga aattcatgac ttaagccagt aactgctttt tgcattgcta
     6721 aaattgttgc ttgataaata ttaacttgat caataacggg agcgtcaaca tgcacaattt
     6781 ggtaatcaag agccttctct ttaattaagt cacttaattc aagtaactcc gttgctgtta
     6841 attgtttcga gtctttaatt cgctcatcgt taaaaaaggg cggcaaaata accgcggcag
     6901 caaacacagg tcctgctaaa gggccgcgtc ccgcttcatc aactcccact aaataaaggt
     6961 gtccgtgttt tcttacatca ttatcaaatt caaaatatgt catattatca ctccaaactt
     7021 acaagctagt aattcaatta ctaacattat tatattatag aaactaacta tttctatttt
     7081 taatatttca aatatttgtg attttgttcc taaaatgcct aagctagtat tgagatttca
     7141 tttttaaaaa ttaaaatgaa aatatagatt agtgaaagga gaagattatg aaaaaacgaa
     7201 aatttttact aatcccacta cttttaggat tgctcttaac tggatgcttt ggtggagacg
     7261 gtcctgaaga tattattgat ggcgacgata aaagcagtca aaaaactact aacgatgtag
     7321 cggaagaaga tattcttgaa aatatggatt acgaaaaaag agaaggctgg ccaacgcaaa
     7381 agcttgctac tttcttatca agcgaaagag ttaatgaaac cgttccagca ttaacaaata
     7441 tcgccactac ttattatcat gaaaataatg acgctaataa taagtacttt gcgattgtag
     7501 tacctggtgc taatcgcgaa gcagattata aagccatact tgaaggagct ggctacacat
     7561 acagtaaact tagttggact agtattgctg ttaacaccga agacacaatt aatattgcct
     7621 accaatacat gcaagatcat ccagttcttg attacgctat gtatattgtt attagtgcgc
     7681 ttaaggctga ttcatttcgc tttgataagc ttgaaagtga tatcggatgg ccggccaatc
     7741 aacttcaaac tttcttaagt aaatacggta ttaccgaaag agttcctagt cttactggtg
     7801 gtaactattt cgctgaatta acagagcgta atgaatttat cgtttgtgcg cctggtgtta
     7861 attttgttga tagctatttc ggcagtcttg aaaatgaagg ttatacagta aatgggtcaa
     7921 acgctgtaag tacttcaggc aaagtgcgga ttaatgtgac cgctaacgat tatccgttct
     7981 tcttctttga tgcgacaaaa attgtcataa aacctgtggt ctaaaactaa ggtctcgcct
     8041 attaaaaagc gaccgttatc cactagcggt cgttttttat atttcactaa tttactactc
     8101 tagactaatt cggccaatta gcccattttt aaattcattt aagattaaga tttcggtgcg
     8161 atcactaaca tcaaaatctt caccaataat cccgcgacgc gtagcaatag cactaatcac
     8221 ggtttggtag ttactatctt ctttaatatc aatatcgtaa aaagctacca tttcttcaaa
     8281 gtaatatgtt tttaagtaat ttactaaata ctctgccatt gtatgcgttg gtaaaatctc
     8341 ttgtctaatt gtacctataa gcgctaattt catgcttgta tcttcttcat cataattagg
     8401 tgataaaatt cctggtgtat caattaaggc aatttcttca ctcactctaa tatactgatt
     8461 agcgcgagta tgccctggtt tattttgcac acttgcggct ttgcgtcccg ctagtttatt
     8521 aataagtgtt gatttaccaa cattaggaat cccgacaatc attaccgcta aaggtcgtgg
     8581 ttttaatccg cgacgcacat cacgttcaat tattggttgc ccgatttctt taatctttgc
     8641 taatatttgt cgcacaaatt tccgttcatt taaattaccc gctaaaacat ttaatttttc
     8701 tgtatgttta cttactcttt taggatcagc taagtcggcc ttagttaaaa cataaagatt
     8761 tggctttcgt tcaataatcg ccgttaagta agtagcgcga ctactatggg ggatccgtgc
     8821 atcgcctaat tcaacgataa tatcgaccat ctttagcgtt tcaatgagct tgcgtgtcgc
     8881 tttttg
//
LOCUS       DAOPCD010000013         8802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_7472_length_8802_cov_4.818452, whole genome shotgun sequence.
ACCESSION   DAOPCD010000013 DAOPCD010000000
VERSION     DAOPCD010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7472_length_8802_cov_4.818452"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /gene="gatB"
                     /locus_tag="PLQ55_00825"
     CDS             complement(<1..1067)
                     /gene="gatB"
                     /locus_tag="PLQ55_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPK28515.1"
                     /translation="MKYEVIIGLEIHIETKTNTKMFSSASSLFDAPANSNVSVVDMAF
                     PGTLPTVNKKAVQNAIMLGHALNMEIDDELHFDRKNYFYSDLPKGYQITQQRRPIGSN
                     GYLDIYVNDTKKRIRIERIHMEEDTAKQIHLGEVTLIDYNRAGNPLVELVTEPDLRSA
                     DEAMAFVEAVREIVTFLEISDGKMEEGSLRCDVNISLRPVGVSKFGNKVEIKNMNSIS
                     NIGKAIETEIKRQSELLDKGLEVESETRRYDDALKDTITMRKKDESVDYRYIPEGNIL
                     PISLSKEFIDATIREMKELPSVKRERYFEEFKLNAHEVNILLQDKDLTSYFDEAARDV
                     KNYKTLINWVIGEFSSYLNKEG"
     gene            complement(1064..2506)
                     /locus_tag="PLQ55_00830"
     CDS             complement(1064..2506)
                     /locus_tag="PLQ55_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HPK28516.1"
                     /translation="MRYLGLTMAELHELYVSGKATPLDVVNEVIEALTKDKNNILEKT
                     MYDEARSMAQSLTKVEKDNLLWGIPFLVKDNIAMKGIETTGSSNILNGFIPLCNAEVI
                     DKLLAAKAIPVAKTTLDEFGMGGRGETGHKGITYNPYGKERTRIVGGSSSGSASGVAS
                     GYVPFAVGSDTGDSIRKPASFAGLVGFKPTWGEISRFGLFAFMPTLDHVGFFTRSVSE
                     ARVLFEKTRGYDKKDATSVMNVPFKTEKDATLKGKKVAVLEPIFALMKNKLLIKEFHN
                     LVDKLRQAGAVVDFVPFDKDLLAAIFPTYFILSCAEANVSNACLTGIGFGNKEEGASY
                     EEIVANTRGKGFSSRIKYRLMLGQYALKKENRNEYYDRAQKARRLIVEAVDKVLENYD
                     VIMLPAAPNHAPTVDKQSEPHSEIENNFMAIANFGGNPSLTLPYFLDEGLPLGVNLTG
                     KPFDDFNVLNIALKIEEITGLYNLHAGLKS"
     gene            complement(2508..2795)
                     /locus_tag="PLQ55_00835"
     CDS             complement(2508..2795)
                     /locus_tag="PLQ55_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28517.1"
                     /translation="MNKEEIRKLAAEILITLTPEEEATFAAEFAYFKELAKLLEALPN
                     IDEKEPMVFPFKVTQTYLKEDTPEESPGAKEILKNSKNAKDTFVVIPKVVK"
     gene            complement(2818..4809)
                     /gene="ligA"
                     /locus_tag="PLQ55_00840"
     CDS             complement(2818..4809)
                     /gene="ligA"
                     /locus_tag="PLQ55_00840"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPK28518.1"
                     /translation="MDYKARILYLREVLNKYNYEYYVLNNSSVSDAEYDSLMQELIAL
                     EKAHPDTFDKTSPSQRVGGFVASEFNKITHSSMMLSLANAFSADDLRDFDRRVRGVIK
                     KDVIDYVVELKIDGLAMSLMYIDGKLSYAATRGDGEVGEDVTSNIVTIKSIPLTLKEE
                     VSFEVRGEVFMPKKSWEELNAIREETGEALFANPRNAAAGSIRQLDSKIAASRKLDAF
                     WYFLVDPLSLNIKTHYDALDYLRTLGFKVNDKIKLCKGIEEVIRHIEKVTSQRHDFPY
                     EIDGIVVKVNDVTKYDELGYTAKTPRWATAYKFPPEEVETILEDIFFTVGRTGKITPN
                     AALKPVFVAGSKIQNATLHNAAFITDRDLKIGDHVFIRKAGDIIPEVLRAVPEKRTGA
                     EKDFVMIENCPVCLEPLTFKDPLHFCLNASCPARYEEGLIHFASRGAMDIEGLGERIV
                     EILFSQRLITNIADIYTLHTKREELMQIEGFGEKSVDNLLNAIEKSKEKSLEKLVFGL
                     GIKELGAKTSKILAEKYMTLDALSCATEAELLTIRDIGPVAASSIVSYFSNEQNLALI
                     TKLKEFGLNTDYLGSVKKDNTFFSGKTIVITGKFENYSRDELTALLENLGAKVAGSVS
                     KKTSLVVAGEDAGSKLTKAQELNIDILNEEQLLKLISEA"
     gene            complement(4812..7013)
                     /locus_tag="PLQ55_00845"
     CDS             complement(4812..7013)
                     /locus_tag="PLQ55_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPK28519.1"
                     /translation="MNLYENLNSKQLEAVTTSGQYVRVIAGAGSGKTRVLTTRIAYLV
                     QEIGTPEDRIVGFTFTNKAAGEMTNRLKKYLERDNVRVRLSTFHSFGARLLREDIDVL
                     GYSRSFTIYDEEDTSSLIRNISVERGHERRGELTRDANNFIGAVKEKGLDAFTYKIRP
                     HGFVNEKEFVEIWKEYERRLKASNCLDFSDLISKSITILEEYRAIREKWQHRFDYILV
                     DEFQDTNDLQYKLIKLLMHQNTSLYVVGDPDQTIYTWRGANERIIMNFDSDFKNAETI
                     ILEENYRSTKYILDAANKLIAHNKNRVEKNLFTAKEKGEPVIVQYTESDSREVNWIYE
                     QIISLRLNVKDFSFRDVAILMRANYLTLPFERHFLHKNVPYRIYGGVRFYQRREIKDV
                     LAYLRILVNEKDDISFSRIVNVPRRGMGNVAMENLKIAALKNEYSLFETVHYVEDIGL
                     SSASQEQLKALMNAVDETRTKLQNDKLELSTVINDYIQKVGYYEHIKTEKDEDKQADM
                     RANVQVLIADIKQFMNDYPESTFNDYLENASLHSAQDEVEEGDYITLMTVHMAKGLEY
                     DYIFVSGLVEGVFPNNRALLDAGRAGEEEERRLCYVAFTRARKRLYLTMSSQYNFAIA
                     SGGRPSRFISEAGLGPPKIKEYHPKYGTPLKEGGVKQVKKEAPRYRANYAEYDYASDF
                     PYAVNDIVEHKTFGSGIVTAIFPTQTTIEVKFDTGEVKRLIASPKYFKEKK"
     gene            7089..7994
                     /locus_tag="PLQ55_00850"
     CDS             7089..7994
                     /locus_tag="PLQ55_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28520.1"
                     /translation="MKMNSLSRTHQLVLGALMGAINVIFALISSYLFAFSLIIMLFLP
                     LASIIVAINIDLKFYPVYLLGTLTLALVLNLGNIDNTLFFLLPILTSGLAFGLLIRHK
                     VPDILILLIVSGVNFLTLLITIPIINLIYDVNFLQVFASFIGFNNIEFGELVLPSILT
                     LLAVMQTLITLVIVTQDAAYFRLEINTEEWPYISLVNLGFSAIVTVLMFFNHGISLAL
                     LFVVILLSLYQIVHLFQKHTIFAWSTLLATIVFIIIGLALFENYTSLPYYFGIIIAVI
                     PVVISDILWLYISRKKSEAKNEGTI"
     gene            7978..8403
                     /locus_tag="PLQ55_00855"
     CDS             7978..8403
                     /locus_tag="PLQ55_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28521.1"
                     /translation="MKGLFKRLGQGILFILALPFGIAIFILFSIYAIITFFYTFFSAI
                     PAFFKGENVFGPTELDIAASTRLAEQKLAAQQPPPPPVVQPSTTINLFTVQQPGTEQV
                     PPPQVTYTQSDGVTYRRVTEQEVAALPEETIIDNSEEQK"
     gene            8400..>8802
                     /locus_tag="PLQ55_00860"
     CDS             8400..>8802
                     /locus_tag="PLQ55_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28522.1"
                     /translation="MILNPYILIKLSESEKRFLIVLFFLIVVVLFVCGLFYEGITRFM
                     ARQGEDIGRLMATQVESGYISSLKQFKRNSYRKSRLEFYRQFIKTFIMMIIWFAIYGT
                     VSIFYRHWLDLFDYTSEGFTTLLWKFDYANTP"
BASE COUNT         2595 a         1759 c         1377 g         3071 t
ORIGIN      
        1 ccttctttat ttaagtacga actaaattca ccaataaccc aattaattag tgtcttatag
       61 tttttaacat ccctcgctgc ttcatcaaaa taacttgtta aatctttatc ttgtagcaag
      121 atatttactt catgagcgtt aagtttaaat tcttcaaaat agcgttcacg ctttacgctc
      181 ggtaactctt tcatttccct aattgttgca tcaataaatt ccttgcttaa agaaattggt
      241 aaaatatttc cttcaggaat atatcgataa tcaacgcttt catctttttt acgcattgta
      301 attgtatctt taagggcatc gtcataccgc cgtgtctcac tttcaacttc aagcccttta
      361 tcaagtaatt cactttgccg tttaatttct gtttcaattg ctttgccaat attagaaatt
      421 gagttcatat ttttaatttc tactttatta ccaaactttg aaactcccac agggcgaagt
      481 gaaatattaa catcacagcg tagtgatcct tcttccattt ttccgtcact tatttcgagg
      541 aatgtgacga tttcacgcac tgcttccaca aacgccattg cttcgtcagc acttcttaag
      601 tctggttctg tgacaagttc cacaagcgga ttaccagcgc ggttatagtc aataagtgta
      661 acttcaccaa ggtgaatctg tttagcggta tcttcttcca tgtggatgcg ttcaatccga
      721 atgcgttttt tagtgtcatt aacataaata tctaaataac cattactacc aattggccgt
      781 ctttgttggg tgatttgata accttttggt aaatcactat aaaaataatt tttgcgatca
      841 aaatgtaatt catcatcaat ttccatattt aaagcgtgac ctaacataat tgcattttgc
      901 actgcctttt tatttaccgt tggtaatgtg ccaggaaaag ccatatcaac aacactaaca
      961 ttgctgttcg caggtgcatc aaaaagacta gaagcgctag aaaacatctt tgtgtttgtt
     1021 tttgtttcaa tatgaatttc taaaccaata attacttcgt atttcatgat tttaaccccg
     1081 cgtgtaagtt gtaaagcccg gttatttctt cgattttaag tgcaatattt aacacattaa
     1141 agtcatcaaa cggtttacca gttaaattaa caccaagcgg caaaccttca tctaaaaagt
     1201 aaggaagggt aagtgaagga ttaccaccaa aattagcaat agccataaag ttattttcga
     1261 tttcactatg tggttcactt tgtttatcta ctgttggagc gtgattcggt gcagcgggga
     1321 gcatgatgac atcataattt tctaatactt tatccactgc ttcaacaatt aagcgacgtg
     1381 ctttttgggc acggtcataa tattcattcc tattttcttt cttcagcgca tattgtccta
     1441 acattaaacg gtatttaatc cgtgaagaaa agcctttgcc ccttgtatta gcaacgatct
     1501 cttcataact tgcgccttct tctttattac caaagccaat ccctgttaaa catgcgttac
     1561 ttacattagc ttcggcacag cttaagataa agtaagttgg aaatatcgcc gctagcaggt
     1621 ctttatcaaa cggaacaaag tccacaactg ctcccgcttg gcgaagttta tcgactagat
     1681 tatgaaattc tttaattaat aatttatttt tcattaacgc aaaaataggt tctaatactg
     1741 caactttttt gccctttaaa gttgcatctt tctctgtttt aaagggaaca ttcattactg
     1801 aagttgcatc ttttttatcg taacccctcg tcttttcaaa aagtacacgc gcttcactaa
     1861 cactgcgggt aaaaaatcca acatggtcta aagttggcat aaatgcgaat agaccaaaac
     1921 gcgaaatttc gccccatgtt ggtttaaagc caacgagtcc agcaaagctt gcgggcttac
     1981 ggattgagtc tcccgtatca ctaccaaccg caaaaggaac atacccacta gcgaccccac
     2041 tagcgctacc gctactagaa ccaccgacaa ttcgtgttct ttctttacca tacggattat
     2101 atgttatccc tttgtgacca gtttcaccgc ggccacccat cccaaattca tcaagtgttg
     2161 tttttgcaac aggaattgct ttagcagcta aaagtttatc aataacttca gcattgcaaa
     2221 gcggtataaa gccgttaagt atattactac tgcctgttgt ttcaatgcct ttcattgcga
     2281 tattatcttt gactaaaaaa ggaattcccc atagtaaatt atctttttca actttagtaa
     2341 gactttgtgc catactgcgt gcttcgtcat acattgtttt ttctaaaatg ttgtttttat
     2401 cttttgttaa ggcttcaatt acttcattaa caacatctaa tggtgttgct ttacccgaca
     2461 catataattc atgaagttcc gccattgtta aacctaggta gcgcatatta tttcaccacc
     2521 tttgggataa cgacaaaagt gtcttttgcg tttttactat tttttaatat ctctttagcg
     2581 ccaggacttt cttccggcgt gtcttctttt aaatatgttt gtgttacttt aaaagggaac
     2641 accattggtt ctttttcatc aatattaggt aaagcttcta aaagcttcgc taattcttta
     2701 aagtaagcaa attctgcggc aaaagtggct tcttcttcag gagtaagcgt tattaatatc
     2761 tctgctgcta actttcttat ttcttcttta ttcataatta tcctcgctag taatatatca
     2821 tgcttcgcta attaatttta gcaattgttc ttcgtttaat atatcaatat taagttcttg
     2881 cgcttttgtt aatttgctac cagcgtcttc gccagcaaca acaagtgaag tttttttact
     2941 aacactgccc gctactttag cgcctaaatt ttctaagaga gcggttaatt cgtcacgtga
     3001 ataattttca aacttacccg taatgacaat tgtcttacca gagaagaaag tattatcttt
     3061 tttaacggac cctagataat ccgtgtttaa gccgaactct tttagtttag taattaacgc
     3121 taaattttgt tcattactaa agtaagacac aattgaagaa gcagcaactg gaccgatatc
     3181 tctaattgtt aaaagttctg cttcagtagc gcaactaagt gcgtctaaag tcatatactt
     3241 ctcggctaga atcttacttg ttttcgctcc taattcttta atccctaaac caaacactaa
     3301 tttttctaaa gacttttctt tacttttttc aatagcgttt aataagttat caactgattt
     3361 ttcaccaaag ccttcaatct gcattaattc ttctctttta gtgtgcagtg tataaatatc
     3421 agcaatatta gtaattaatc tttgtgagaa aagaatttct acaattcttt cgccgagccc
     3481 ctcaatatcc attgcgccgc gactcgcaaa atgaattaag ccttcttcat aacgcgcagg
     3541 acacgacgca tttaaacaaa aatgcagcgg gtctttaaag gtaagcggtt ctaaacaaac
     3601 cggacaattt tcaatcatta caaaatcttt ttcggcaccc gttctttttt ctggtacagc
     3661 gcgcaatact tctggaataa tatcaccagc tttacgaata aaaacatggt cacctatttt
     3721 taagtcacga tcagtgataa acgctgcatt atgtaaagta gcgttttgaa tctttgaacc
     3781 cgcgacaaat actggcttaa gtgcagcgtt tggtgttatt ttacctgttc ttccaaccgt
     3841 aaagaaaata tcttcaagta ttgtttcaac ttcttcgggc gggaatttat aagcagtagc
     3901 ccaccgtggc gttttagcgg tataaccaag ttcgtcatac tttgtaacgt cattaacttt
     3961 cacgacaata ccgtcgattt cataagggaa atcatgtcgt tgcgatgtta ctttttcaat
     4021 atgccgaatt acttcttcaa tccctttaca taatttaatt ttgtcattta ccttaaatcc
     4081 taaagtgcgt aaataatcaa gagcatcata atgcgttttt atatttagtg aaagcggatc
     4141 aactaagaaa taccaaaaag catcaagctt ccgactagca gcaattttag aatcgagttg
     4201 acgaattgag cccgcggcgg cgttacgcgg attagcaaaa agtgcttcac ccgtttcttc
     4261 acgaatagca tttaattctt cccaagattt tttaggcata aatacttcgc cacgcacttc
     4321 aaaactaact tcttctttaa gtgttaaagg aatactttta attgtgacaa tatttgaagt
     4381 aacatcttcc ccaacttcac cgtctcctct agttgccgca taacttaact ttccgtcgat
     4441 atacattaag ctcatcgcta agccgtcaat ctttaactct acgacataat caatgacatc
     4501 ttttttaata acaccgcgga cacgacgatc aaaatcacgt aagtcatcag cactaaaggc
     4561 attcgcaagt gaaagcatca ttgaactatg tgtaatctta ttaaattcac ttgcaacaaa
     4621 gccaccaact ctttggctcg gacttgtttt atcaaaagta tcgggatgcg ctttttcaag
     4681 ggcaattaat tcttgcatta aactatcata ttcagcatca ctaacactcg aattattaag
     4741 gacataatat tcatagttat acttatttaa aacttcgcgt aaataaagaa ttcttgcttt
     4801 ataatccatc tttatttttt ctccttaaag tattttgggc tagcgattag tcgctttact
     4861 tcgccagtat caaacttaac ttcaattgtt gtttgcgttg gaaaaatggc agtaacaatt
     4921 cctgatccaa atgttttatg ttccacaata tcgttaactg cataaggaaa atcactagcg
     4981 taatcgtatt ctgcataatt agcgcgataa cgcggcgctt cttttttaac ttgtttaaca
     5041 ccaccttctt tgagcggtgt cccgtatttt ggatgatatt ctttaatctt cggtggtcct
     5101 aaccccgctt cactaataaa gcgtgatggt ctcccgccag aagcaatcgc aaagttatat
     5161 tgcgaactca ttgttaaata aagccgttta cgcgctctag taaaagcaac gtaacatagt
     5221 cttctttctt cctcttcacc agcgcgacca gcatcaagca acgctcggtt atttggaaag
     5281 acaccttcaa caagtcctga aacaaaaata taatcatact caagaccttt tgccatatgc
     5341 acagtcataa gcgtaatata atcaccttct tcaacttcat cttgggcgct atgtaaacta
     5401 gcgttctcta aataatcatt aaaggttgat tcaggataat cattcataaa ttgtttgatg
     5461 tcggcaatta agacttggac gttagcacgc atatctgctt gtttatcttc atctttttcg
     5521 gttttaatat gttcgtagta accaactttt tgaatatagt cattaataac agtacttagt
     5581 tcaagtttgt cattttgtaa ttttgttcgt gtctcatcca cagcattcat taacgctttt
     5641 aattgttctt gtgaagcact tgataagcca atatcttcaa cataatgcac tgtttcaaat
     5701 agtgaatatt cattctttaa cgcagcaatt ttaaggtttt ccatcgctac attacccatg
     5761 ccacggcgcg ggacattaac gattctgcta aaagaaatat catctttttc attaactaaa
     5821 atccgcagat acgctaaaac atctttaatc tcgcgtcttt gatagaaacg cacgccacca
     5881 taaatacggt acggaacatt cttatgtaaa aagtgccgtt caaatggtag tgttaagtaa
     5941 ttagcgcgca taagaattgc gacatcacga aacgaaaaat ctttgacgtt taagcgcaat
     6001 gaaataattt gttcataaat ccaattaact tcgcgtgaat cgctttctgt atattgaaca
     6061 atcactggtt cacctttttc tttagcggta aaaagatttt tctccactct atttttgtta
     6121 tgagcaataa gtttatttgc tgcatccaaa atatatttag tggaacgata gttttcttct
     6181 agaataatcg tttcggcatt tttaaaatca ctatcaaaat tcataatgat tcgctcgtta
     6241 gcgccacgcc atgtataaat tgtttgatcg ggatcaccca cgacatagag tgatgtattt
     6301 tggtgcatta aaagtttaat gagtttatat tgcaaatcat tagtgtcttg aaattcatca
     6361 acgagaatat aatcaaagcg atgttgccat ttttctctaa ttgctctata ttcctctaaa
     6421 atcgtaattg atttactaat tagatcacta aaatcaagac agtttgacgc tttaagtctt
     6481 cgttcatatt ctttccatat ttcaacgaat tctttttcat taacaaagcc gtgcggtcta
     6541 attttataag taaaagcatc taagcctttt tctttaaccg ccccaataaa attatttgcg
     6601 tcacgcgtta attcgccgcg gcgttcatgt cctctttcta cacttatatt gcgaattaag
     6661 gaacttgtat cttcttcgtc atatattgta aagctgcgtg aatagcctaa aacatcaatg
     6721 tcttcacgca gtagtcttgc accaaaagag tgaaatgttg aaagacgcac acgaacatta
     6781 tcgcgttcta aatacttttt taagcgattt gtcatttcgc cagcggcttt atttgtaaaa
     6841 gtaaaaccga caatgcggtc ttccggagta cctatttctt gcactaaata agcaatacga
     6901 gtagtgagta ctcgcgtttt accacttccc gcaccagcaa taacacgcac atattgcccg
     6961 cttgtagtaa cagcttctaa ctgttttgaa ttaagatttt catacaaatt catatctaaa
     7021 atattataac ataatataag gtgaaattac tcccgtagtg aagccctttt ttatgttaaa
     7081 atatgactat gaaaatgaac tcattatcac gcacacatca actcgtttta ggcgccctca
     7141 tgggtgcgat aaatgtaata tttgcactta tcagcagtta tttatttgct tttagtttaa
     7201 ttattatgct ttttttaccg ctggcgagta ttattgtcgc aattaatatt gatttaaaat
     7261 tttatcctgt ttatttacta ggtacgctta ccttagcgct tgtattaaat cttggtaata
     7321 ttgataacac actttttttc ttattaccga tattaacaag cggattagcc tttgggcttt
     7381 taattagaca caaagtaccc gacatcttaa ttttattaat cgttagcggt gtgaactttc
     7441 ttacactttt aattacaata ccgattatta atttaattta tgatgtgaat ttcttacaag
     7501 tgttcgctag ttttatcggt tttaataata ttgaatttgg tgaattagtg ttgccttcaa
     7561 tcttaacctt attagcggtt atgcaaacac tcattacgct tgtgattgta acgcaagacg
     7621 ctgcttattt tagattagaa attaatacgg aagagtggcc ttatattagt ttagttaatt
     7681 taggatttag cgctattgtt acggtattaa tgttttttaa tcacgggatt tcgcttgcgc
     7741 ttttgttcgt tgttattttg ctttcactct atcaaatcgt gcatcttttc caaaaacaca
     7801 ccatttttgc atggtcaaca ttacttgcga caattgtgtt tattattatt ggtttagcat
     7861 tatttgaaaa ttatacttcg cttccttatt attttggtat tattattgcg gtaattccag
     7921 ttgtgattag cgacattttg tggttatata ttagtagaaa gaaaagcgag gcaaaaaatg
     7981 aagggactat ttaaaagatt aggtcaagga attcttttta tcctagcgct accatttggg
     8041 atagcgatct ttatcctttt ttcaatttac gccattatta cttttttcta tactttcttt
     8101 tctgctattc cagcattctt taaaggtgaa aatgtcttcg gtccaaccga attagatatt
     8161 gccgctagta cacgcttagc cgaacaaaaa ctagcagcgc aacaaccacc accgccacca
     8221 gtcgtgcaac cttcaacaac tattaactta tttacagtac aacaacccgg caccgaacaa
     8281 gtgccaccac cacaagtcac ttatacccaa agtgatggtg ttacttatcg ccgtgttact
     8341 gaacaagaag ttgcagcact tcctgaagaa actattattg ataatagcga ggagcaaaaa
     8401 tgattttaaa tccgtatata ttaattaagc ttagtgaaag tgaaaaaagg tttttaatcg
     8461 ttctcttttt cctaattgtc gttgtgcttt ttgtatgcgg actattttat gaaggtatta
     8521 cgcgctttat ggcacgccaa ggtgaagata ttggcagact catggcaaca caggtggaat
     8581 cgggttatat atcttcactt aaacaattta aaagaaactc ttaccgtaaa tcacgcttag
     8641 aattttatcg tcaatttata aaaacattca ttatgatgat tatctggttt gcgatttatg
     8701 gtactgttag tatcttttac cgtcactggt tagatttatt tgattacact agtgaaggtt
     8761 ttactaccct cttatggaaa tttgattacg ctaatacgcc ac
//
LOCUS       DAOPCD010000014         8772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_7513_length_8772_cov_4.982448, whole genome shotgun sequence.
ACCESSION   DAOPCD010000014 DAOPCD010000000
VERSION     DAOPCD010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8772
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7513_length_8772_cov_4.982448"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..634
                     /gene="rplD"
                     /locus_tag="PLQ55_00865"
     CDS             59..634
                     /gene="rplD"
                     /locus_tag="PLQ55_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPK28523.1"
                     /translation="MQLNGEIFGIEPHQDVVHQAVVTYLANRRQATAKTKTRAEVRGG
                     GKKPWRQKGTGRARAGSRRSPIWVGGGVTFGPTGEQNYKLSMNKKAHALALRSALSIK
                     AAEKAFIIIDDTIEIEGKTKAAAELLKNVGATGKVLLINDDNKSLLLATRNLPNVQSV
                     GQDNVAVYDLLNADQIVVEKATLVAYVGGLE"
     gene            634..978
                     /gene="rplW"
                     /locus_tag="PLQ55_00870"
     CDS             634..978
                     /gene="rplW"
                     /locus_tag="PLQ55_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPK28524.1"
                     /translation="MSKRPRKKAEQVVQVKKPTVEQFNLLLRPVITEKSMALMQDQNK
                     VTMEVHKTANKESVKDAFEAVFGVTVTDVKIINTNSKKTRRGGRYYGTIPGVKKAVIT
                     VAEGQAIDLFKE"
     gene            999..1832
                     /gene="rplB"
                     /locus_tag="PLQ55_00875"
     CDS             999..1832
                     /gene="rplB"
                     /locus_tag="PLQ55_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPK28525.1"
                     /translation="MAIRKYKPTTPSRRNMTNLTFEQITKTKPEKSLTVELKRTGGRN
                     NMGKITTRHIGGGHKRRYRIIDFRREKDGVEGVVAAIEYDPNRNANIALIHYLDGEKR
                     YILHPQGLAVGDRIVSGEAVDIKVGNAMPLAAIPEGSVVHNVELTPGKGGQIARAAGT
                     SVQILGREGKYAILRLQSGEVRKVLQACRATVGQVGNEDYNLINLGKAGKSRWKGIRP
                     TVRGSAMNPVDHPHGGGEGKSPIGKDAPRTPWGKRALGVKTRKQNKKSTQLIIRRRKQ
                     K"
     gene            1846..2145
                     /gene="rpsS"
                     /locus_tag="PLQ55_00880"
     CDS             1846..2145
                     /gene="rpsS"
                     /locus_tag="PLQ55_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPK28526.1"
                     /translation="MARSLKKGPFADKSLLKKVDELNKANKKQIIKTWSRRSTIFPSF
                     VGHTFAVYNGREHITVYVTEDMVGHKLGEFSPTRSYRGHTGHTAEDKAAAAQRKR"
     gene            2153..2560
                     /gene="rplV"
                     /locus_tag="PLQ55_00885"
     CDS             2153..2560
                     /gene="rplV"
                     /locus_tag="PLQ55_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPK28527.1"
                     /translation="MARIKKPKTTEKVETKEVKEKPVVTEARAISRSVGVTPRKMRLV
                     ADLVRGKKVSDAIALLLNINKAASVPLRKTIESAAANAINNHGFKEEGLYISELQIND
                     SIKLKRYRPRAKGGAAGVTKRTSTIIVKVKESV"
     gene            2574..3239
                     /gene="rpsC"
                     /locus_tag="PLQ55_00890"
     CDS             2574..3239
                     /gene="rpsC"
                     /locus_tag="PLQ55_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPK28528.1"
                     /translation="MGQKVNPNGLRYGIYRNWNSRWFAKNKDFATFLKEDMDIREFLT
                     KELKEALLSHIEIDRVNTFESGYSVIVSVHVARVGVVIGQQGERVKELTAKLAKIVKS
                     GEVKINVVDIKNPDLNASLVAQDIARQLEARGSFRIVQKRTIQRVRRAGAKGIKTLVS
                     GRLNGAEIARSEGYKDGIIPLHTLRADIDYALAEAHTTYGRLGVKVWICRGEYDLSKG
                     RGE"
     gene            3242..3664
                     /gene="rplP"
                     /locus_tag="PLQ55_00895"
     CDS             3242..3664
                     /gene="rplP"
                     /locus_tag="PLQ55_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPK28529.1"
                     /translation="MLQPKRTKYRRPQRLSYEGLTKGGANIEFGEFGLKALEGAYITN
                     RQIESARIVLASYTRRVGKIWIRIFPHLAKTKKPLEVRMGSGKGAPDSWVAVVKKNRI
                     LFEIGGVKEEIAREALRLAAYKLPIKTRVVSKADNKED"
     gene            3667..3864
                     /gene="rpmC"
                     /locus_tag="PLQ55_00900"
     CDS             3667..3864
                     /gene="rpmC"
                     /locus_tag="PLQ55_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002878201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPK28530.1"
                     /translation="MKIKEIRALSTTELKEKQYLLIEELFHLRRKQAVGQLENGKEIT
                     RVRKDIARVYTILRERELGIN"
     gene            3879..4142
                     /gene="rpsQ"
                     /locus_tag="PLQ55_00905"
                     /pseudo
     CDS             3879..4142
                     /gene="rpsQ"
                     /locus_tag="PLQ55_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010580226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            4204..4578
                     /gene="rplN"
                     /locus_tag="PLQ55_00910"
     CDS             4204..4578
                     /gene="rplN"
                     /locus_tag="PLQ55_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002878195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPK28531.1"
                     /translation="MIHVETKVNIADNSGARIGKVIRLYGGSVRRHSNIGDIVLITVR
                     QAVPNSKVKKGDMVKAIVVRTKKGVLRPDGSKIKFDDNAVVIINNDGNMIGTRVFGPV
                     ARELREKGDGAMKIVSLAPEVL"
     gene            4589..4897
                     /gene="rplX"
                     /locus_tag="PLQ55_00915"
     CDS             4589..4897
                     /gene="rplX"
                     /locus_tag="PLQ55_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPK28532.1"
                     /translation="MLIKKGDKVIVITGEDKGKTGIVQRAYPKLQKVVVEGVNVRKKH
                     KKPTQQNPEGSILDVYAPIHVSNVALIDPKTKKPTRVGMKEVKGKKVRYAKKSGEVIK
                     "
     gene            5211..5762
                     /gene="rplE"
                     /locus_tag="PLQ55_00920"
     CDS             5211..5762
                     /gene="rplE"
                     /locus_tag="PLQ55_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010580223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPK28533.1"
                     /translation="MKKSTNRVVRQYEDVAIKALMEKFNYTSVMQVPKLSKIVINMGV
                     GDATQNAKLLEDAVRELTVIAGQKPVVTIAKKSEASFKLRAGTAIGCKVDLRGERMYD
                     FFEKLISIALPRVRDFRGVSKDSFDGHGNYTLGVREQLIFAEIDFDLVNKVRGMDIVI
                     VTTAKTDAEAYELLKLLGMPFRR"
     gene            5777..5962
                     /locus_tag="PLQ55_00925"
     CDS             5777..5962
                     /locus_tag="PLQ55_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004268593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPK28534.1"
                     /translation="MAKTSKRVAQLRKPKFKVRAYSRCSRCGRPHSVYRKFGVCRICL
                     RELAYNGEIPGMKKASW"
     gene            5978..6373
                     /gene="rpsH"
                     /locus_tag="PLQ55_00930"
     CDS             5978..6373
                     /gene="rpsH"
                     /locus_tag="PLQ55_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPK28535.1"
                     /translation="MSMTDPIADMLTRIRNANALSHEAVAMPSSKNKVNIARILKEEG
                     YITDYVVSGDVKKVLTLVLKYGPNGEKVISGLKRISKPGLRVTTTSDKLPRVLRGLGI
                     AIISTSQGIMTDKKARALGVGGEVVAYVW"
     gene            6384..6932
                     /gene="rplF"
                     /locus_tag="PLQ55_00935"
     CDS             6384..6932
                     /gene="rplF"
                     /locus_tag="PLQ55_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPK28536.1"
                     /translation="MSRIGLKVIKVPAGVEVKLEGDLITVKGPKGTNHVTLPLHITLD
                     QSHEGEIHVKRDNELKQTKQSHGTTRALLHNAIVGAHEGFTIRLQIVGIGYRAIQEGE
                     SVRLEVGYSHPTVIAPCVGAKITVENPNNVVVTGYDKQAVGQTAALIRGVRRPEPYKG
                     KGIKYSDEVIIRKEGKRAGAKK"
     gene            6943..7299
                     /gene="rplR"
                     /locus_tag="PLQ55_00940"
     CDS             6943..7299
                     /gene="rplR"
                     /locus_tag="PLQ55_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPK28537.1"
                     /translation="MVKQIKRNEIRKRRHARIRRKISGTHEVPRLVVFRSNKHIAVQL
                     IDDTKGVTLGSASSLAMKLVHGDNVESAAKVGTEIANVAKNLGITKVVFDRGGYLYHG
                     RIKALADAARKAGLEF"
     gene            7481..7957
                     /gene="rpsE"
                     /locus_tag="PLQ55_00945"
                     /pseudo
     CDS             7481..7957
                     /gene="rpsE"
                     /locus_tag="PLQ55_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003194373.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            7968..8408
                     /gene="rplO"
                     /locus_tag="PLQ55_00950"
     CDS             7968..8408
                     /gene="rplO"
                     /locus_tag="PLQ55_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPK28538.1"
                     /translation="MKLHELTYTEGAKRARKRLGRGIGSGTGKTSGKGEKGQKSRSSG
                     GTRLGFEGGQMPLYRRIPKRGFTNMNTRKFAVVNLEELNVFEDGTVVTPTLLIEKGII
                     KQELGGVKVLARGELTKKLTVKAHRFSKQASDAIAKLGGTAEVI"
     gene            8410..>8772
                     /locus_tag="PLQ55_00955"
     CDS             8410..>8772
                     /locus_tag="PLQ55_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPK28539.1"
                     /translation="MKKIAAIFKNKEIVDRIFFTIMIMFVFRIGAAITVPGVTLSDFS
                     FQNNDAFGLMNLLGGGTLQQFSVFALGVSPYITASIIVQLLSMDVLPALTDLANQGDY
                     GRKKMEMTNRYLTLMLGAV"
BASE COUNT         2971 a         1757 c         2052 g         1992 t
ORIGIN      
        1 agtaaagaaa actattactt atccagttgt taacactaaa ggggaaaaag ttggtacaat
       61 gcaacttaat ggcgaaatct tcggtattga gccgcatcaa gatgtcgtcc atcaagcagt
      121 cgtgacttac cttgctaacc ggcgtcaagc taccgcgaaa acaaaaacac gcgcagaagt
      181 acgcggtggt ggtaagaaac catggcggca aaaaggaaca ggccgtgccc gcgctggtag
      241 tcggcgttca cccatctggg ttggcggtgg agtcaccttt ggtccaaccg gagaacaaaa
      301 ctacaaatta tcaatgaata agaaagcgca cgcgctcgca ttaagaagtg cgctctcaat
      361 caaagctgcg gaaaaagcat tcatcattat cgatgacacc atcgaaattg aaggcaaaac
      421 aaaagccgct gcggaattac ttaaaaatgt tggggccact ggtaaagtct tattaattaa
      481 tgacgacaat aagagcttac tacttgccac ccgcaactta ccaaacgtcc aaagcgttgg
      541 tcaagacaat gtcgccgttt atgacttatt aaacgctgac caaatcgtcg tcgaaaaagc
      601 aaccttagtt gcgtatgtag gaggacttga ataatgagta aacgtccacg caaaaaagca
      661 gaacaagtcg tacaagttaa aaaaccaacg gttgaacaat tcaacctctt gcttagaccc
      721 gtcattactg aaaagtcaat ggcgctcatg caagaccaaa acaaagtaac gatggaagtg
      781 cataaaaccg caaataaaga aagtgttaaa gatgcatttg aagcggtctt cggtgtaaca
      841 gttaccgatg taaagatcat taacacaaat tcaaaaaaaa cacggagagg tggtcggtac
      901 tacggcacaa tcccaggcgt caaaaaagct gtgattaccg ttgctgaagg acaagcaatt
      961 gacctcttca aagaataaag acgggaagga gaaacattat ggcaattaga aaatataaac
     1021 cgacaactcc ctcacgtcgg aacatgacga accttacgtt cgaacaaatt acgaaaacaa
     1081 aaccagaaaa aagcttaacc gtcgaactta aacgcactgg tggtcgtaat aacatgggaa
     1141 aaattactac ccgtcacatc ggtggtggtc ataaacgcag ataccgcatt attgacttcc
     1201 gtcgggagaa agatggtgta gaaggcgttg tcgcggcaat cgaatacgat ccaaaccgca
     1261 acgcaaatat cgccttaatc cactacttag acggtgaaaa gcgttacatc ttacatccac
     1321 aaggactcgc ggtcggcgat cgcatcgtct cgggtgaagc agtcgacatc aaagtcggta
     1381 acgcaatgcc gctcgcagca atcccagaag gtagtgtcgt acataacgtt gaattaaccc
     1441 ctgggaaagg cggacaaatc gcccgcgctg cgggaacaag tgtccaaatc ttaggacgcg
     1501 aaggaaaata tgcaatcctt cgactccaaa gtggtgaagt acggaaagta ttacaagctt
     1561 gtcgcgctac agtcggacaa gttggtaatg aagactacaa cttaatcaac ttaggaaaag
     1621 ctggtaagag tcgctggaaa ggcattcgcc caacagttcg tggctctgcg atgaacccag
     1681 ttgaccaccc acacggtggt ggtgaaggga agtcaccaat tggtaaagac gcaccaagaa
     1741 caccatgggg taaacgcgcc ttaggtgtga aaacgcgcaa acaaaataag aaatcgacgc
     1801 aattaattat cagacgtcgc aaacaaaaat aaggagtgag gaaacatggc tagaagttta
     1861 aaaaaaggtc cattcgctga taagtctctc ttaaaaaagg ttgacgaatt aaataaagcg
     1921 aataaaaaac aaattattaa aacatggtcg cgcagatcaa ccatcttccc atccttcgtc
     1981 ggtcatacat tcgcagtgta taacggacgt gaacacatta cagtgtatgt gacggaagat
     2041 atggtcggcc ataaattagg cgaattctcc ccaacccgct cataccgcgg acatacgggg
     2101 cacacagctg aagacaaagc cgcggccgca caacggaaac ggtaggtgaa ttatggcaag
     2161 aattaaaaaa ccaaaaacaa cagaaaaagt tgaaacaaaa gaagttaaag aaaaacctgt
     2221 ggttaccgaa gcacgcgcaa tcagtcgcag cgtcggagtc accccacgga aaatgcgctt
     2281 agtcgctgac ttagtccgcg ggaaaaaggt gagtgatgca atcgcgctct tactaaatat
     2341 taataaagcc gcgagtgtac cgcttcgcaa aacaatcgaa agtgccgcag cgaacgccat
     2401 caacaaccat ggcttcaaag aagaagggct ctacattagt gaattacaaa ttaatgattc
     2461 gattaaatta aaaagatatc gcccgcgcgc taaaggcgga gcggctggtg tcactaaaag
     2521 aacaagcaca attattgtga aagtaaaaga aagcgtgtaa aggaggaaaa gtaatgggac
     2581 aaaaagttaa tccaaacggt ctaagatacg gtatctatcg taactggaat agtcgttggt
     2641 ttgccaaaaa caaagacttc gctactttcc tcaaagaaga catggacatt cgtgaattct
     2701 taacgaaaga attaaaagaa gcattactct cacatattga aattgatcgc gtgaacacat
     2761 tcgaaagtgg ctatagtgtc atcgtcagtg tccacgtcgc ccgtgtcggt gtcgtgattg
     2821 gtcaacaagg cgaacgcgta aaagaattaa ccgctaaatt agccaaaatt gtgaagtctg
     2881 gtgaagttaa aattaatgtc gttgatatta aaaacccaga cttaaatgca agtttagtgg
     2941 cgcaagacat cgcgcgtcaa ctcgaagcac gtggttcatt tagaatcgtg caaaagcgga
     3001 cgatccaacg cgtccgtcgc gctggcgcta aaggaattaa aactttagtc agcggtcgct
     3061 taaatggtgc ggaaatcgcc cgtagtgaag gttataaaga cggaatcatc ccgcttcata
     3121 ccttacgcgc tgatattgat tacgcacttg cagaagcaca cacaacttac ggccgtcttg
     3181 gtgtaaaagt gtggatttgt cgtggtgaat acgacttaag taaagggaga ggagaataat
     3241 tatgttacaa ccaaaacgta ctaaatatcg tcgcccccaa cgcttgagtt atgaaggctt
     3301 aaccaaaggt ggcgcaaata tcgaatttgg cgaatttgga cttaaagcac ttgaaggtgc
     3361 ctacatcact aaccgtcaaa tcgaaagtgc acggattgtg cttgctagtt atacacgccg
     3421 tgttggtaag atctggattc gtatcttccc acacttagcg aaaactaaaa aaccacttga
     3481 agtgcggatg ggttccggta aaggggctcc agactcatgg gtcgccgttg tcaaaaagaa
     3541 tcgtattctc tttgaaatcg gtggcgttaa agaagaaatc gctcgtgaag cactccgcct
     3601 cgcagcgtac aaattaccaa ttaagacacg cgtagtgtct aaagcagaca ataaggagga
     3661 ttaatgatga agattaaaga aattcgtgcg ttaagtacaa ccgaattaaa agaaaaacaa
     3721 tatcttttaa tcgaagaatt atttcacctc cgtcggaagc aagccgttgg tcaattagaa
     3781 aacggtaaag aaattactcg cgtccgaaaa gatatcgcgc gtgtatatac tatattacgc
     3841 gaacgcgaac tcgggattaa ttaaggagca gtgataatat ggaacaaaca gtaagaaaag
     3901 cacgcgctac gtttgacggg attgtcatta gtgataaaaa tgataaaaca atcaccgtct
     3961 tagtagaaac atacaaacgg cataagattt atggaaagcg cgttaaatat cgtaaaaaat
     4021 attacgctca cgatgaagaa aatgtggcaa aagaaggcga cttcgtacgc atcatggcca
     4081 cacgcccaat gagtgccctt aaaagatggc ggctcgtcaa agtcttagaa cttgcggaag
     4141 tgcaacttga agacattatc gacgaagaag gcgaactcat cgaagagcta aaggaggcaa
     4201 gtgatgattc acgttgaaac taaagtaaac atcgctgata actccggtgc ccgaatcgga
     4261 aaagttatta gactctatgg tggcagtgtc cgtcggcaca gtaacattgg cgacatcgtc
     4321 ctaattacag tcagacaagc cgtccctaac tccaaagtta aaaaaggtga catggtgaaa
     4381 gcgattgtcg tccgcactaa aaaaggtgtg ttacggccag acggatcaaa aattaaattt
     4441 gatgataacg ctgttgttat tattaataat gacggcaaca tgatcgggac acgggtgttt
     4501 ggccccgtag ctcgtgaatt acgtgaaaag ggagatggcg caatgaaaat tgttagcctt
     4561 gctccagaag tattatagga gggctcgtat gttaattaaa aaaggtgata aagtcatcgt
     4621 catcactggc gaagacaaag gcaaaaccgg aattgtccaa cgggcgtatc cgaaattaca
     4681 aaaggttgtt gtggaaggcg ttaatgttcg caagaaacat aaaaaaccaa cccaacaaaa
     4741 ccctgaagga tcaatcttag atgtgtatgc gccgattcat gtgagcaatg tcgcacttat
     4801 tgatccaaaa acaaaaaaac caacacgcgt tgggatgaaa gaagtgaaag gcaaaaaagt
     4861 tcgctacgct aaaaagtcgg gcgaagtaat taaataggag gtgcattatg gcagaagata
     4921 aagcaaagaa aactgttgca aaagaagatg ctccaaaaaa agcagctcct aaagccgaaa
     4981 ctaaaaaagt agaaaagaaa gccgctgctc ctaaaaaagc agaagcgaaa actactgaaa
     5041 agaaagaaac cgcgaaagct gaagtaaaaa aagcagcgcc aaaaaaagaa gcagcaaaag
     5101 ctgaaacgaa aaaagctgcg ccgaaagccg aagctaaagc ggaagttgct aaagaaaaac
     5161 cagccaaaaa ggttgaaaaa gaacataaag ttgaacgtta tgtccatgaa gtgaaaaaga
     5221 gcacaaatcg tgttgtccgt caatatgaag atgtagcgat taaagcttta atggaaaagt
     5281 ttaactacac aagcgtgatg caagtcccaa aattaagcaa gattgtcatt aatatggggg
     5341 ttggggatgc aacacaaaac gctaaattat tagaagacgc agtgcgggaa ttaaccgtca
     5401 ttgccggtca aaaaccagtg gtgacaattg cgaagaaaag tgaagcaagc ttcaaacttc
     5461 gtgctggaac cgcaattggt tgtaaagttg atttacgcgg cgaaagaatg tatgacttct
     5521 tcgagaagtt aattagtatc gcgctcccac gtgtgcgtga cttccgcggt gtcagtaaag
     5581 atagcttcga cggtcacggg aactatacac tcggagtccg cgaacaacta atcttcgctg
     5641 aaattgactt tgacttagta aataaagtcc gcgggatgga catcgttatt gtcacaaccg
     5701 ctaaaacgga tgccgaagca tatgaattgc ttaaactact cggtatgccg tttagacgct
     5761 agaggaggga gagattatgg caaaaacaag taaaagagta gcgcaattac gcaaacctaa
     5821 atttaaagta cgcgcctata gtcgttgttc acgttgtggc agacctcata gcgtttatcg
     5881 caaattcggt gtgtgccgta tttgtttacg tgaactcgct tataatggcg aaattcctgg
     5941 catgaagaag gccagctggt aaggaggagc aactagtatg agtatgacag atccaattgc
     6001 agatatgcta acccgcattc ggaacgctaa tgctttaagt cacgaagcag ttgcgatgcc
     6061 gagcagcaaa aacaaagtta atattgcccg tatcttaaaa gaagaaggtt atattactga
     6121 ttacgtcgtt agtggcgacg ttaaaaaagt tttaacctta gtgcttaaat acggaccaaa
     6181 tggtgaaaaa gtaatttctg gtttaaaacg tatttctaag ccaggcttaa gagtcaccac
     6241 taccagcgat aaattaccac gcgttttacg cggtttaggt atcgctatta tttccacatc
     6301 acaaggaatc atgactgaca agaaagcgcg cgccttaggc gtcggtggcg aagtagtcgc
     6361 ctatgtgtgg taggaggtaa gttatgtcaa gaattggttt aaaagtaatt aaagtgccag
     6421 caggcgtaga agtgaaactt gaaggtgact taatcaccgt taaaggacca aaagggacaa
     6481 atcacgttac cttaccactc catatcaccc ttgatcaaag tcatgaaggt gaaattcatg
     6541 ttaaacgcga caatgaatta aaacaaacaa aacaaagtca tggtacaacc cgtgctttac
     6601 tccacaatgc aattgttgga gcgcatgaag gttttactat tcgcttacaa atcgtcggta
     6661 tcggttaccg cgctattcaa gaaggcgaaa gtgtccgctt agaagtcggt tacagccatc
     6721 caactgtaat tgctccatgt gttggtgcta aaattacagt ggaaaaccca aacaacgttg
     6781 ttgtgacggg ttatgacaaa caagctgttg gtcaaaccgc agcgctcatt cgcggtgtcc
     6841 gtcgtccaga accttataaa ggtaaaggaa ttaaatatag cgacgaagtt attattcgta
     6901 aggaaggaaa gcgcgcaggc gccaagaaat aggtgaaaag ttatggttaa acaaattaaa
     6961 cgtaatgaaa ttagaaaacg gcgtcatgcc cgtattcgcc gcaaaattag cggtacacac
     7021 gaagttccgc gccttgtcgt cttccgttcc aacaaacaca ttgctgtgca attaattgat
     7081 gacactaaag gtgttacctt aggtagcgct agttcattag cgatgaaatt agtgcatggc
     7141 gacaatgttg aatcagcggc aaaagtcggt actgaaattg ctaatgttgc gaaaaactta
     7201 ggcattacta aggttgtgtt cgatcgcgga gggtatttat accatggtcg aatcaaagcc
     7261 ttagccgatg ctgcccgtaa ggccgggtta gaattctaga aggagtaaag ttatggataa
     7321 agaaaagaaa aacttagtag aagatactga agaagttgaa actgaagaag tagaaactga
     7381 agaaaaagat accgaacaaa ctccgcaagg tgatgccccg cgccgtagta atagaggtgg
     7441 ccgtcgtgaa ggtggtccgc gtcgtggtgg ccgtcgtgcc ccagcgcgtc gcccaaaaga
     7501 atttgaagaa cgcgtcgtcg cgattaaccg cgttagtaaa acaacaaaag gtggccgtcg
     7561 cttacgcttt actgccttag tcgttattgg ctatggtaaa gggaagtatg gctacggaaa
     7621 cgctaaagca gcagaagtac ctgatgcgat tcgtaaagca attgaagttg caaagaaaaa
     7681 caccttcaca gttgaacttg ttaagggtga cacaattgct cacgaaatcg ttgttcgctt
     7741 cggtgcaacc cgcgtattct taaaaccagc accagaaggt actggagtta tcgccggtgg
     7801 ttcagtgcgg gccatcttag aacttgcagg tattcgcaac gtctattcaa aagtttatgg
     7861 ttcgcgtacc gcccttaatg tgcttaaagc aacagataac ggattacgcg gtttaaaacg
     7921 agcttcggat attaaacgtc tccggggaac attgtaggag gtagagtatg aaattacacg
     7981 aacttactta tacagaagga gcaaaaagag ctcgtaaacg tctaggccgg gggattggct
     8041 ctggcacagg taaaacaagt ggaaaaggtg aaaaaggtca aaagtcacgc tcaagcggtg
     8101 gcacgcgtct cggttttgaa ggtggacaaa tgcctttata tcgccgtatt cccaaacgtg
     8161 gctttacaaa catgaataca cgaaagtttg ccgtcgttaa tttagaagaa ttaaatgtct
     8221 ttgaagacgg aactgttgtg accccaacac tcttaattga aaaagggatc attaaacaag
     8281 aactcggtgg cgttaaagtc ttagcgcgcg gagaattaac aaagaaatta acagttaaag
     8341 cccacagatt ttccaaacaa gctagtgacg ccattgctaa actaggtgga accgcagagg
     8401 taatttaata tgaaaaagat cgccgcgatt ttcaagaata aagaaattgt cgatcgtatc
     8461 ttctttacga tcatgattat gtttgtcttc cgaattggcg ccgcgattac ggttccaggc
     8521 gtcacgctta gcgacttttc attccaaaat aacgatgcct ttgggcttat gaatctcctt
     8581 ggtgggggca cactgcaaca atttagtgtg ttcgcacttg gggttagtcc ttatattacc
     8641 gcatcaatta tcgtccaact cttatcaatg gacgtccttc ctgcgcttac tgaccttgct
     8701 aatcaagggg attatgggcg gaagaaaatg gaaatgacaa atcgttactt aacgctgatg
     8761 ctcggcgcag tg
//
LOCUS       DAOPCD010000015         8758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_7524_length_8758_cov_4.548776, whole genome shotgun sequence.
ACCESSION   DAOPCD010000015 DAOPCD010000000
VERSION     DAOPCD010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8758
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7524_length_8758_cov_4.548776"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLQ55_00960"
     CDS             complement(<1..273)
                     /locus_tag="PLQ55_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28540.1"
                     /translation="MSLKFSTSNESAIRAYIAGDKEAINLILANFQYFIRSRAGLLAS
                     LHDKSGIDYFDLELIGQSALITAVRTYRADATPFAPFATVVINNAMS"
     gene            complement(322..1053)
                     /gene="rlmB"
                     /locus_tag="PLQ55_00965"
     CDS             complement(322..1053)
                     /gene="rlmB"
                     /locus_tag="PLQ55_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPK28541.1"
                     /translation="MNNNNQRTQLAYGRNNVLACLDAGIVIKIIFQEGFSDEKIIKLA
                     TKQNLEITYAKKKDLNNMVAGNHQGIVAVVKSFKFNILEELIIAANNVDNPIILVLDE
                     IKDPHNLGAIIRSAEAFGVTGIVMKKDRQVSITPAVMKVATGAQNNILIAQVTNLSQA
                     LEKLKKTGFWVVSTDLKAEQNVSDLKYDFKTALIMGNEHKGVSPLLTKNSDFVVKIPM
                     LGKTNSLNVSVATGVMLALIRQIQL"
     gene            complement(1053..2387)
                     /gene="cysS"
                     /locus_tag="PLQ55_00970"
     CDS             complement(1053..2387)
                     /gene="cysS"
                     /locus_tag="PLQ55_00970"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPK28542.1"
                     /translation="MKILLYNSLTKQKEEFIPLRKGEVSMYVCGPTVYDYVHVGNMRP
                     VVVFDTLRRFFNHAGYQVIYVSNYTDVDDRIIEKAQKTNKKETEISEFYIEAFADSAT
                     KINALKPTFTPKATQHIESMIAFIKVLIANGAAYENDGEVFFRVSKVETYGELSNVTQ
                     DELQVGARIDANPKKEHPNDFLLWKKTDDAGIKWDSPWGAGRPGWHTECVVMINDIFG
                     KPLIDIHGGGFDLKFPHHENEIAQARAYANTGLANYWMHNGFINLNNQKMAKSTGNFV
                     TAKDFIEEYGATSLRLLLLSTHYRSPVNLSAEIIENTNNEVLRLQRTKQALVRHFALH
                     PLKVTDDSLLLKFEEALADDLNTPNALSELYAVVKDLNILLRNPLKEEEKLAKLANAF
                     EVMLDVLGLDLGVKNLNEEDTKLLKDYENARKNKDFAKSDALREELQKRGIF"
     gene            complement(2434..3096)
                     /locus_tag="PLQ55_00975"
     CDS             complement(2434..3096)
                     /locus_tag="PLQ55_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPK28543.1"
                     /translation="MNYKHIIFDLDGTLLDTIPDLLYNLNTTFSELGLPGKFSTAEMA
                     TFLGSGKDEQIRRALSARRLSHEKYFAKVNALLSVYYAKNTNTYTEIFPGIKETLAEL
                     KARCVKLYVATNKPDHVAKDVVRHYFGDNVFIKVRGDKGDGIIKPNPKFLNAIIKNIN
                     DPLETIMFVGDSYVDYMSAKNAGVKCAIVPHGYDQKVLNIKDKGVIFLKKVTDIISLD
                     IN"
     gene            complement(3099..3641)
                     /locus_tag="PLQ55_00980"
     CDS             complement(3099..3641)
                     /locus_tag="PLQ55_00980"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HPK28544.1"
                     /translation="MRISINTKVSFGVTVFTSVALIIIGAMIVKYNTNLAASLFYLIG
                     IIIALVGFINFLNYISGRKQKVTVSEYLLSILLMIAGILVAIFSESIQAYGLLVIGIL
                     FVMLGIYDIVLYFRSKATITLIVGILRIVIGTAFIISGFSEVLLANDEFSRKLWLIIG
                     YASIVLGVTFLVLDTFQVTE"
     gene            complement(3782..4183)
                     /gene="rplQ"
                     /locus_tag="PLQ55_00985"
     CDS             complement(3782..4183)
                     /gene="rplQ"
                     /locus_tag="PLQ55_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002818480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPK28545.1"
                     /translation="MPAQGRKNVRGKAGVRFKAAYTKSKDKAMIRNVTSELIVYGKVT
                     LIAKVGKQVQREVERLITLAKRGDLHARRLAAARLREGIKRADGVKALDVLFNELGPR
                     FKDREGGYTRILKLGPRRGDNAPMVILEFVE"
     gene            complement(4193..5158)
                     /locus_tag="PLQ55_00990"
     CDS             complement(4193..5158)
                     /locus_tag="PLQ55_00990"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003641267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPK28546.1"
                     /translation="MIPFNPTVFKEKNYYATDNYGKFVLEPLERGFGQTLGNSFRRLL
                     LSALPGASVFAITIDGARHEFATLPGVVEDVTMITLNLKQLILKIEDKDTTIKRLEVD
                     VVGPAVVTGADITLPYGVEVISKDLVIANVAEGGHLKMTIFARNGRGYVTSESNKAER
                     QKVGGAIGVIATDSNYSPVTKVNYTVSNTRVGHDENYDRLTLEVWTNGSITPQAAVAL
                     SAKILIEHYKPFLDLEEATVDVEVFAEQEAQAPAQMEDKKIEELDISVRSYNCLKRAG
                     IQTVLELTSKTEEEILKVRALGKKSFKEIKDKLKELGLNFRGSVE"
     gene            complement(5180..5578)
                     /gene="rpsK"
                     /locus_tag="PLQ55_00995"
     CDS             complement(5180..5578)
                     /gene="rpsK"
                     /locus_tag="PLQ55_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPK28547.1"
                     /translation="MSKKARRSTTRRRRIRRSYTACIAHIHSTFNNTIVTITDEDGNA
                     ISWSSAGALGFKGSKKSTAWAAQLAGEAAAKVAVEQGLKTVDVRVKGPGQGREAAIRA
                     LALAGLTITGIEDTTPIPHNGCRPPKRPRG"
     gene            complement(5599..5976)
                     /gene="rpsM"
                     /locus_tag="PLQ55_01000"
     CDS             complement(5599..5976)
                     /gene="rpsM"
                     /locus_tag="PLQ55_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPK28548.1"
                     /translation="MARIVGVDIPNDKRVVVSLQYIYGIGLTTAKKILAAVKIDENVR
                     VKDLTEEELGAIRQEITKYRVEGDARRQVALNIKRLTEIGSYRGIRHRRGLPVRGQRT
                     KTNARTRKGPKKTIANKKQASQG"
     gene            complement(5988..6101)
                     /gene="rpmJ"
                     /locus_tag="PLQ55_01005"
     CDS             complement(5988..6101)
                     /gene="rpmJ"
                     /locus_tag="PLQ55_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPK28549.1"
                     /translation="MKVRASVKPICDKCRVIRRKGNVMVICENPKHKQRQG"
     gene            complement(6124..6342)
                     /gene="infA"
                     /locus_tag="PLQ55_01010"
     CDS             complement(6124..6342)
                     /gene="infA"
                     /locus_tag="PLQ55_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPK28550.1"
                     /translation="MSKSNVITVEAKVIECLPGVNFRVQLDNGHIILAHVSGKIRRYN
                     IRILPGDRVAVEISPYDLTRGRITYRYR"
     gene            complement(6342..7100)
                     /gene="map"
                     /locus_tag="PLQ55_01015"
     CDS             complement(6342..7100)
                     /gene="map"
                     /locus_tag="PLQ55_01015"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPK28551.1"
                     /translation="MISQKSPREVRLMKESGRIINLVFKEVEAKLRPGMSTADVDKLA
                     LEVITREGGTPTFKGYGGFSGNICVSVNDTLIHGIPSHREIIKDGDIVSVDVGVTKDG
                     YVADAARTFLVGNVSENAKRLVEVTEESFWYAVDNFAKPGNYIGDISHAIYEYASSRG
                     YTLTRDYTGHGVGRKLHEAPAVPNVGTPKTGPRLKVGMTLAIEPMLNEGLVDLIVLKD
                     GWTVKTKDGKLASHYENTIVITEDGYEVLTMEEK"
     gene            complement(7093..7752)
                     /locus_tag="PLQ55_01020"
     CDS             complement(7093..7752)
                     /locus_tag="PLQ55_01020"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPK28552.1"
                     /translation="MCNIILLGPPGAGKGTLAKQLISVYNIPHISTGDMFREAIKEGT
                     PLGRLAASYINEGHLVPDEVTISLVRERLSQGDCANGYLLDGFPRTLPQAEALDTIGK
                     DLGRPINVVINITADETELVRRISGRRVCRKCGAPYHIDTMKPKKDGVCDACGGELYQ
                     RKDDNKDNLLVRLNHYFADTKPLEDFYAEHGVLYAVDSTAGAKKSLKAAQKIIEGAKC
                     D"
     gene            complement(7757..>8758)
                     /gene="secY"
                     /locus_tag="PLQ55_01025"
     CDS             complement(7757..>8758)
                     /gene="secY"
                     /locus_tag="PLQ55_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPK28553.1"
                     /translation="LMLGAVQAYGVIVTMENQSYITLKESNFWTYAYIITVMLAGTML
                     VMWLGDQISSKGIGNGISMIIFAGIVSSLPQQIIRAYQNWLSDKVGQGSQQLFIGLLQ
                     FGVYVLSFLAIIGFITFIETSKRKIPVQHAGKSGQTRKMAQASFLPIKINSAGVIPVI
                     FASSIMMAPSIIASFISGSNLDAEWLKIFNSSATVTMPWFNDKTWQMPWGLLIYLFLI
                     VVFAFFYAKLQINPEKLAENFQKNGSYIPGVRPGAETERYVSKVLNRITVIGAFSLAV
                     IAALPIILVLLKVVPDQSLALGGTGLIIVVGVAIEINNQIDGLLAGKSYEEGSVLGG"
BASE COUNT         2488 a         1892 c         1548 g         2830 t
ORIGIN      
        1 agacatcgca ttattaatga caacggtggc aaaaggagca aagggagttg catcagctct
       61 ataagttcta accgcagtaa taagtgcact ttgaccaatt agttctaaat caaaataatc
      121 aataccactt ttatcatgaa gtgacgctag taaaccagcg cgacttctta tgaaatattg
      181 aaaattagca agaattaaat taattgcttc tttatcacca gcgatataag cacgaatagc
      241 gctttcgttt gatgttgaaa attttaaaga catggcagga acctttctat taagtaattt
      301 attttaagtt actattaata actacaattg aatttgacga attagtgcaa gcattacgcc
      361 tgttgcgaca cttacattta aagaattagt ttttccaagc atcggaatct taacaacaaa
      421 atcagaattt ttcgttaata aaggtgaaac gcctttatgc tcattaccca ttatcagtgc
      481 agttttgaaa tcgtatttta aatcgctaac attttgttca gcttttaagt ctgtacttac
      541 aacccagaac ccagtctttt ttaatttctc taatgcttgc gaaagattag tcacttgcgc
      601 aattaaaata ttattttgcg ctccagtagc aactttcatt accgctggag taattgagac
      661 ttgccgatct tttttcatca ctattcctgt gacaccaaaa gcttcagcac ttctaattat
      721 cgccccaaga ttatgtggat ctttaatttc atctaatact aaaataattg gattatctac
      781 attattcgca gcaataataa gttcttccaa aatattaaat ttaaaagatt taacaacggc
      841 gacaatacct tgatgattgc ccgcaaccat attatttaaa tcttttttct ttgcataagt
      901 aatttcaaga ttttgcttag tggcaagttt aattatcttt tcatcactaa aaccttcttg
      961 aaaaataatt ttaattacaa tccccgcatc taaacacgct aaaacgttat ttcgtccgta
     1021 ggctaattgt gttctttggt tattattatt cattaaaaaa taccccgttt ttgcaactct
     1081 tcgcgtaaag cgtcactttt cgcaaaatcc ttgtttttcc gtgcattttc gtagtctttt
     1141 agcaatttag tgtcttcttc atttagattt ttaacaccta aatcaagtcc taaaacatca
     1201 agcattactt caaatgcgtt tgctaattta gcgagttttt cttcttcttt taacgggttt
     1261 cgaagtaaga tatttaagtc tttaacaact gcatataatt cacttaaagc gtttggtgta
     1321 tttaagtcgt cggctaaagc ttcttcaaat tttaagagta gtgaatcatc tgtaactttg
     1381 agtgggtgta gcgcaaaatg acgcacaagt gcttgcttag ttctttgtag gcgcagcact
     1441 tcattattag tgttttcaat tatctctgca ctaaggttta ctggactccg ataatgcgta
     1501 gataaaagta ataagcgtaa tgatgtagcg ccatactctt caataaaatc tttagcagtt
     1561 acaaagttac cagtggactt tgccatcttt tgattgttta aattaatgaa gccgttatgc
     1621 atccagtaat tagcaagccc cgtattagcg taagccctcg cttgcgcgat ttcgttttcg
     1681 tgatgtggaa acttaagatc aaatccgcca ccatgaatat caattaaagg cttcccaaat
     1741 atatcattaa tcatcacaac gcattctgtg tgccaccctg gcctacctgc gccccacggt
     1801 gaatcccatt taattcctgc atcatccgtt ttcttccata ataagaaatc attcggatgt
     1861 tcttttttcg ggttagcgtc aatccgcgca ccaacttgaa gttcgtcttg tgtgacattt
     1921 gacaattcac cataagtttc aacttttgac acacggaaaa atacttcacc atcattttca
     1981 taagcagcgc cattagcgat taacactttt ataaaagcaa tcatactttc aatgtgctgc
     2041 gttgcttttg gggtaaatgt aggttttaag gcattaattt tagttgccga gtctgcaaaa
     2101 gcttcaatat aaaattcact aatttcagtt tcttttttat tagttttttg cgctttttca
     2161 atgattctat catcaacatc agtgtagtta gaaacataaa tcacttgata acctgcgtga
     2221 ttaaaaaagc gccgtaatgt atcaaagacg acaacaggac gcatattacc aacatgcaca
     2281 tagtcgtaga ctgttggacc gcatacatac attgatactt cgcctttacg cagcggaatg
     2341 aattcttctt tttgttttgt aagtgagttg tataaaagta ttttcatagt aatattttat
     2401 cacgaacaca ggtaattaac gcactctatg ttcctaatta atgtctaaag aaataatgtc
     2461 tgttactttc tttaaaaaga ttacaccttt atctttaatg tttaacactt tttgatcgta
     2521 accgtgcggt acaatagcgc attttacacc ggcatttttc gctgacatat aatcaacata
     2581 actatcgccc acgaacataa ttgtttcaag cgggtcatta atgtttttaa taatcgcatt
     2641 taaaaatttt ggattaggtt taataatccc gtcaccttta tcgccacgta ctttaataaa
     2701 aacattatca ccaaaataat gtctcactac atctttagcg acgtgatctg gcttattagt
     2761 ggcaacatat aacttaacac agcgcgcttt taattccgct agtgtttctt ttatgccggg
     2821 gaatatttcg gtataagtat tcgtgttttt ggcgtaatac accgacaaaa gggcattaac
     2881 tttcgcaaaa tacttttcgt gactaagccg tcttgcactg agcgctcttc ttatttgttc
     2941 gtctttaccg ctccctaaaa aagtcgccat ttccgcggtg ctaaatttac ccggtaaacc
     3001 taattcacta aaagtagtgt ttaagttata gagtaaatca ggaatcgtat ctaaaagcgt
     3061 tccatctaaa tcaaaaataa tatgtttata attcatagct attcagtaac ttggaatgta
     3121 tcaagcacaa ggaaagtcac gcctaataca attgacgcat aaccaataat aagccacagt
     3181 tttcgtgaaa attcatcatt agcaagcaat acttcactaa acccagaaat aataaaggcc
     3241 gtaccaataa caatccgtaa aatgccaaca atcagtgtta ttgtcgcttt agagcggaaa
     3301 tataaaacaa tatcatatat tcctaacatc acaaataaaa tcccaatgac aagtaagccg
     3361 taagcttgaa tactttcact aaaaatagcg actaaaattc cggcaatcat tagtaagata
     3421 ctaagtaaat attccgagac cgtaactttc tgtttacgcc cggaaatata attcaagaaa
     3481 ttaataaagc caacaagggc aataataatc ccaattaagt aaaaaagact tgccgctaaa
     3541 ttagtgttat atttaacgat catcgctcca ataataatta gtgcgacact agtgaagact
     3601 gttactccaa aactaacctt tgtatttatt gatatgcgca tacttttctc ctttattcct
     3661 ctttaatatt atattaaaca cacgcttttt gcaaagtatt atcactttaa gtgatatgcc
     3721 tttttaaaca aaaaagccta accttaatgg ctaggcttaa tgaattttag taagacgcgt
     3781 cttattcgac gaattctaag atgaccatcg gggcattgtc accacgacgt gggcctaact
     3841 taagaatgcg ggtataaccg ccttcacgat ctttaaagcg tggtccaagt tcattaaaga
     3901 gtacatcaag tgctttgaca ccgtctgctc ttttaattcc ttcacgtaac cgcgcggcgg
     3961 caagtctgcg tgcatgtaag tcaccgcgtt tagcaagtgt gattaagcgt tcaacttcgc
     4021 gttgaacttg tttcccgacc ttagcgatta aagttacttt accgtaaact ataagttcac
     4081 tcgtaacatt acgaatcatc gctttgtctt tactttttgt atacgccgct ttaaagcgga
     4141 ctccggcttt accccggaca ttttttctac cttgtgctgg catattgtac ctctattcta
     4201 cactgcctcg gaagttaagg ccgagttctt taagtttgtc tttaatttcc ttaaatgatt
     4261 ttttacctaa agcacgaact ttaagaattt cttcttcggt tttacttgta agttcaagta
     4321 ctgtttgaat tccagcgcgc ttcaagcagt tgtaagaacg gacactgatg tcgagttctt
     4381 caatcttctt atcttccatt tgtgctggcg cttgtgcttc ttgttcagcg aagacttcaa
     4441 cgtcaacagt tgcttcttct aaatcaagga aaggtttata gtgctcaatt aagattttgg
     4501 cactgagggc gaccgcagct tgtggggtaa tactaccatt agtccacact tcaagcgtta
     4561 agcggtcata attttcatcg tgtccgacac gagtgtttga cacggtgtaa ttaacttttg
     4621 tcactggtga ataatttgaa tcagtggcga tgacaccaat tgcaccacca actttttgcc
     4681 gttcggcttt attactttcg ctagtgacat aacctctgcc gttgcgagca aagattgtca
     4741 tttttaagtg accaccttca gcgacattag cgatcactaa atctttacta ataacttcga
     4801 caccgtaagg aagagtaata tccgctccag tgaccactgc tggccctaca acatcaactt
     4861 ccaaacgttt aattgtcgta tctttatctt caatcttgag aatgagttgt ttaaggttaa
     4921 gtgtaatcat cgtcacgtct tcgacaacac caggaagtgt tgcaaattca tgacgtgcgc
     4981 cgtcaatcgt aatagcgaag acacttgcac caggaagggc acttaagagt aaacgacgga
     5041 atgaattacc gagcgtttgt ccaaagcctc tttcgagtgg ttcaagcaca aacttgccat
     5101 agttatctgt tgcgtaataa tttttttcct taaaaacggt gggattgaat gggatgataa
     5161 tattactcat gttgcctcct tatccgcgtg gccgtttcgg gggccgacac ccgttatgtg
     5221 gaattggggt tgtatcttca attccggtaa tcgtaagtcc cgctaaagcg agtgcgcgaa
     5281 tagcggcttc tcggccttgt cctggacctt taactctaac atcgaccgtt ttaagtcctt
     5341 gttccactgc gactttcgct gcagcttcac cggctaattg cgcggcccat gcagtgcttt
     5401 ttttactacc tttgaaacct aatgcaccag cgctactcca gctgatggcg ttgccgtctt
     5461 catcagtaat cgtaacgata gtgttattaa aggttgagtg aatatgcgcg atacaagcgg
     5521 tataagaacg acgtattctg cgtcttcttg tcgtacttct gcgtgctttt tttgacataa
     5581 ctaactttca ctccttcctt aaccttgtga cgcttgcttt ttattagcaa tcgttttctt
     5641 cggtccttta cgtgttcttg catttgtttt tgtgcgttga ccacgaactg gtaatccgcg
     5701 acgatgacga atcccgcggt atgaaccgat ttctgttaat cgtttaatat ttaatgctac
     5761 ttgccgtctg gcgtcacctt cgacgcggta cttcgtaatt tcttgacgaa ttgcaccaag
     5821 ttcttcttcg gttaaatctt taacacgaac attttcatca attttaacag cggctaatat
     5881 ttttttagcg gttgttaagc caataccata aatgtattgt aagctcacta cgacccgctt
     5941 atcgtttgga atatctacac caacaatccg tgccatattc tttcctccta tccttgtctt
     6001 tgcttatgct tagggttttc gcaaatgacc atcacattgc ctttgcggcg aataacccgg
     6061 catttatcgc aaattggttt gactgaggct cttactttca taatgaattt cctccaattt
     6121 cttttatcga taccgatatg taatgcggcc ccgtgttaaa tcatagggcg aaatttcaac
     6181 tgcgacacga tcacctggta gaatgcgaat gttatacctt ctgattttac cagaaacgtg
     6241 ggccaaaata atgtgtccgt tgtctaattg aacgcggaaa ttaactcccg gtaaacattc
     6301 aatgactttc gcctcgacgg taattacgtt cgatttactc attacttctc ctccatagtt
     6361 aatacttcgt agccatcttc ggtaataacg atggtgtttt cataatggct cgctaattta
     6421 ccatcttttg ttttgacggt ccagccgtct tttaagacga taaggtcgac caacccttcg
     6481 tttaacattg gttcaatcgc taaggtcata cccactttta agcggggacc agtctttggc
     6541 gttccaacgt ttggtacagc gggtgcttca tgtaatttac gccccacacc gtgacccgtg
     6601 taatctcgcg taagtgtata cccacgactt gacgcatatt cataaattgc gtgactgata
     6661 tctccgatgt agttccctgg ttttgcaaag ttgtcgactg cataccagaa gctctcttcg
     6721 gtaacttcga cgagtctttt tgcgttttcg ctaacatttc cgactaagaa tgtccgtgct
     6781 gcatccgcaa catacccgtc ttttgtcacc ccgacgtcaa cactcacaat gtcgccgtct
     6841 ttaataattt cgcggtggct tgggattccg tggattaatg tatcattaac cgaaacacag
     6901 atattgccac taaatccgcc ataacccttg aaggttgggg tgccaccttc ccgagtaatg
     6961 acttcaagcg ctagtttatc aacatcagca gttgacatcc caggtcttaa cttagcctct
     7021 acttctttaa agactaagtt aataatccga cctgattctt tcattaagcg cacttcgcgg
     7081 ggtgattttt gactaatcac atttagctcc ttcaataatt ttttgtgcag cttttagtga
     7141 ttttttcgcc ccagcagtac tatcaacagc gtagaggaca ccatgctctg catagaagtc
     7201 ttcaagtggc tttgtatcag caaaatagtg atttaagcgc actaagagat tgtctttatt
     7261 atcatctttg cgttgatata attcaccacc acaagcatca cacacaccgt cttttttcgg
     7321 cttcatcgta tcaatgtggt atggagcgcc gcatttacga catactctgc gcccactgat
     7381 gcgccgcact aattcggttt catcagcagt aatattaatt actacattaa ttggccgacc
     7441 taagtctttg ccaattgtgt cgagtgcttc ggcttggggg agggttcgtg ggaacccgtc
     7501 taacaagtaa ccatttgcgc aatctccttg cgataagcgt tctcttacga gactaattgt
     7561 tacttcatct ggcactaagt gcccttcatt tatataactt gccgcaagtc tgccaagtgg
     7621 agtgccctct ttaattgctt cacggaacat gtcacctgta gagatatgcg gaatgttata
     7681 gacactaatt agttgtttgg caagtgttcc tttaccagcc cctgggggtc ctaataagat
     7741 aatattacac atacgcctaa cctcctaata cgcttccttc ctcgtaagac ttaccagcga
     7801 gaagcccatc aatttggtta ttaatttcaa ttgccacccc aacgacaatg attagtcctg
     7861 ttccccctaa ggctaagctt tggtcaggaa cgactttaag taagactaag ataattggta
     7921 gggccgcaat aaccgctaaa ctgaaggcac caatgacggt aatacggtta agtactttag
     7981 acacatagcg ttccgtttca gcacccggac gaacaccagg aatataacta ccattctttt
     8041 ggaagttttc cgcgagcttt tccggattaa tttgtagttt agcgtagaag aaggcaaaca
     8101 caacaataag gaataagtaa atgagtaatc cccacggcat ttgccaagtc ttatcattaa
     8161 accacggcat tgttactgtc gcgcttgagt taaagatttt taaccattcg gcgtctaaat
     8221 ttgagccgct gataaaagaa gcaataattg acggcgccat cataatactt gaagcaaaga
     8281 tgacagggat aaccccggca ctattaatct taattggtaa gaaacttgct tgcgccatct
     8341 tgcgggtttg cccgctttta cccgcgtgtt gcacgggaat tttacgttta ctcgtttcaa
     8401 taaaggtaat aaaaccaata atggcgagga aactaagtac ataaactcca aattggagga
     8461 gaccgataaa taattgttga ctgccttgtc caactttatc actaagccaa ttttggtagg
     8521 cacgaataat ttgttgggga aggctgctaa cgatacctgc gaagataatc atcgagatac
     8581 cattaccaat ccctttacta ctaatttggt caccaagcca cattacaagc atcgtgcccg
     8641 caagcatgac ggtaataatg taagcatatg tccaaaagtt tgattctttc agcgtaatat
     8701 aactttgatt ttccatcgta acaataacgc cataagcttg cactgcgccg agcatcag
//
LOCUS       DAOPCD010000016         8651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_7620_length_8651_cov_3.877966, whole genome shotgun sequence.
ACCESSION   DAOPCD010000016 DAOPCD010000000
VERSION     DAOPCD010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8651
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7620_length_8651_cov_3.877966"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="PLQ55_01030"
     CDS             <1..1426
                     /locus_tag="PLQ55_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HPK28554.1"
                     /translation="ITSQIFRGFANGLEGKETMNAVQFGEAMLVGKDVAYKAVMRPVE
                     GTMLTVIREAAEVLNEKAKPDWSIVKALEVMVAEAKASLERTPDLLPVLKEVGVVDSG
                     GAGVVAILEGMLAAITTGIIQKESATATEVVPQYEMAGARITDEEEYGYCTEFILRIG
                     AGEEVKKTFNEERFASVLNSQGNSIVMVRDEDILKVHIHTLRPGTTLTYAQQFGEFVT
                     LKIENMTEQHTHLSDHGKDYFNTNPQVTSASMEMKDYALISVASGEGLSDLFKEYRVD
                     YVVKGGQTMNPSTEDFLAAIKKVHAKTVFILPNNGNIILAATQAAEVTNEEGVVNVHV
                     IPSKTIPQGLVAAMNFNPEVDTEHNIEIMTEALDAVETGQITFAIKDTSIDGFEVKKD
                     YYMGLQGKKILCSLPDKFDALRELLAGMIDELTAIVTIIYGEDVEESAAEELLAELEE
                     KYSAVDFELVNGGQPVYSFIVGLE"
     gene            1435..3399
                     /locus_tag="PLQ55_01035"
     CDS             1435..3399
                     /locus_tag="PLQ55_01035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPK28555.1"
                     /translation="MAKISNSSRIINALAKYDIHTFRDVIFHLPRRYLDMSYTDEFKM
                     NTNEKVTLKLKVTSAPLLIKPQRVAIIRFTAVSESGATYKIVAYNQRYILNAVKIGDL
                     ITVYGTINLRLLELTVIKLFPRVLSEEEQLSPLYSLPSEVGNHEFIRIVQKALKIESV
                     TSRVPRPLADKNGLISLNDALKRVHNPRDLNDVFEGLKALKYEEGLEYFIKLLLIKRA
                     NSELTNSKKTIVNLKDANEFVKRLPFKLTKDQLTAVREIGYDMNKETLMYRLLQGDVG
                     SGKTVVSFVALYINYLRGAQGVLLAPTESLAKQHYETLLSFFKDFDLRIELLIGALTP
                     SVKTKLKEDIQHGLVDIVVGTHALFSEDVFYPKLELVVIDEQHKFGVNQRNTLLNKGE
                     KADLLLLSATPIPRTLANVIYGDMDISTIESFPHTGRQVNTIITTSASKNVIPLIDKT
                     LSENRQVYIVAPKISFGDKTNVLALYERFSARYPHLVGMLHGELASEEKDAALLNFKS
                     GVTPILVSTTVVEVGIDVKSAGLMIIYEPHSFGLSSLHQLRGRIGRDGKRATLCLVSE
                     EEDNYKLEEFAREDNGFKIAELDLALRGPGELTGEKQSGMPSFMYLNVIKDLKLITII
                     KEDALEVINNPTNPHYFPLLKVINDALEKD"
     gene            3650..4636
                     /gene="plsX"
                     /locus_tag="PLQ55_01040"
     CDS             3650..4636
                     /gene="plsX"
                     /locus_tag="PLQ55_01040"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPK28556.1"
                     /translation="MKLVIEMMGGDYGFESTIPALIQFKKDYPEVELFAVGRKEDLAP
                     VADYATIVDARDVVKIDATPLEVLRAKESSLLKAINTVIEEKADALISAGGTGPYLSA
                     ATLKLKLIEGVERAAILAPFPTKIKGKQVAILDIGASNENTASHLYQFALMGQQYARA
                     VFGVEAPKTYLLSNGTEDTKGSPLVLEAFKLYKEKELPYFMGQMEARDALNGEADVVI
                     ADGFTGNVLLKSIEGTAKVLMDSVKGAFKRNLFSKLGYLFARKGFKNMAETFDYKNTG
                     GAILIGVNNVVVKAHGNSDPRGFYSAILVGYKMAKADIVNKVREGLKNVETK"
     gene            4620..5300
                     /gene="rnc"
                     /locus_tag="PLQ55_01045"
     CDS             4620..5300
                     /gene="rnc"
                     /locus_tag="PLQ55_01045"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPK28557.1"
                     /translation="MSKLNSLFTKLGITPSNEGLFEEALTHSSYKTKDKDAHDYERLE
                     FLGDAILNMLIASAIYKSHPDKKQGELTNMRKNFVRTQTLATLGRKLELERYIRTGKS
                     ITPQDLYSNDKYFEDVFEALIGAMYLDQGLKVTEDFILRMLGSAIEQYDFNQIFDYIS
                     TLQEFMQADKRGLPQYITEPSGYDNKEEAFISTVTYDGIILGCGYGANSKEAEQNAAK
                     NALEKVAR"
     gene            5302..6282
                     /gene="ftsY"
                     /locus_tag="PLQ55_01050"
     CDS             5302..6282
                     /gene="ftsY"
                     /locus_tag="PLQ55_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPK28558.1"
                     /translation="MGLISFLKKKFGGKKNETTDKYVVGLEKSKANFSSKLKRLAKRY
                     EKVTEEYYEHLLEILIGADVGVTLTYEIIEATKEEALLNNLTDPEAINDLLVDKMFIG
                     YLKQGEDIVNEIQFADNGPTVLLVVGVNGSGKTTSIAKLAHRYKKEGKKVLLVAGDTF
                     RAGAVAQLEVWAKRLDVPIVTGKPNADPASVAYDGVAEGKKINADLIIIDTAGRLQTK
                     VNLMAELSKIRRVIGDQIENAPHEVFLIIDATTGQNGVLQAKAFAEATGLTGVVITKM
                     DGTSKGGIILAIRDEIGVPVRFIGLGEKMEDLDEFDLEKYLYGLCLGEDE"
     gene            6282..6560
                     /locus_tag="PLQ55_01055"
     CDS             6282..6560
                     /locus_tag="PLQ55_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28559.1"
                     /translation="MVVISLLSLVVSFLVFMGVRLAVYSLNPSNYLFADILIIIIGSF
                     IVSLIFAIRQFRYMGRDKWRSQFFHGFVLRTFLINFAIIFIISLLWSW"
     gene            6566..6892
                     /locus_tag="PLQ55_01060"
     CDS             6566..6892
                     /locus_tag="PLQ55_01060"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28560.1"
                     /translation="MDELKEYMYINELLLIYGKTLTKRQLKIMESYYKYNLGVQEIAE
                     ELNITKAAVSDAIKVSTKHLENLESIVGHYAYRQQVEALHEEIKNTSTLQEVKELLSK
                     EEDDGI"
     gene            6879..8225
                     /gene="ffh"
                     /locus_tag="PLQ55_01065"
     CDS             6879..8225
                     /gene="ffh"
                     /locus_tag="PLQ55_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPK28561.1"
                     /translation="MMAFESLTERFSKILKKVRGQSRLTEKNMDEMLQEVRIALFEAD
                     VNFKVVKEFVNNIKEKALGTKVYDTLNPGQMVVKIVNDELVELLGSTDTELIFEKNKP
                     TIIMLVGLQGTGKTTTAGKLALHFKKKMNKRVLLAAADVYRPAAIDQLDTLAKQVGVD
                     IVNLGDKVSPVEIAKAAKKKAYDDRYDVLIIDTAGRLTIDETLMNELKEMKAAVTPTE
                     ILLLVDAMSGQDAVNTALSFNEKLGLTGAIMSKLDSDARGGAALSIKHLTGVPIKFAG
                     VGETLEDLDIFYPDRMADRILGMGDVLTLVEKAQQQLDEKTVKKTTRKIMEGEFDLND
                     MLEQMKQVQKLGKLGGILKLIPGMPKITPEQQELAEREMKLFEVIINSMTPEERSNPA
                     ILRNSRKVRIAKGSGTSNQEINKVIKKYEQMKQMMKQMKGYKKGGPMPPGFPGMGR"
BASE COUNT         2972 a         1359 c         1842 g         2478 t
ORIGIN      
        1 gattactagc caaattttcc gcgggttcgc taatgggctt gaaggtaaag aaacaatgaa
       61 cgctgtccaa tttggtgaag caatgttagt cggtaaagat gtcgcttaca aagcggttat
      121 gcgtcctgtt gaaggcacaa tgctcaccgt tattcgtgaa gcggctgaag tgttaaacga
      181 aaaagctaag cctgattggt caattgttaa agcgcttgaa gtaatggttg cggaagctaa
      241 agcaagttta gagcgtaccc ctgatttatt accagtttta aaagaagttg gcgtcgtcga
      301 tagtggtggc gctggggttg tggccatttt agaaggaatg cttgcggcca ttactaccgg
      361 tattattcaa aaagaaagtg caaccgcaac ggaagttgta ccgcaatatg aaatggcggg
      421 agcgcggatc actgatgaag aggaatacgg ttactgtact gaatttatct tacgaattgg
      481 cgctggtgaa gaagtaaaga aaacatttaa tgaagagcgt tttgcaagcg tcttaaattc
      541 acaaggtaac tcaattgtga tggtacgcga cgaagatatc ttaaaagtgc acattcatac
      601 tttaagacca gggacaactt taacttatgc gcaacaattt ggggaatttg tcacgcttaa
      661 aattgaaaat atgacggaac aacatacgca cttaagtgat cacggcaaag attattttaa
      721 cactaatccg caagtgacta gtgcaagcat ggaaatgaaa gactacgctt taatttctgt
      781 cgctagtggt gaaggattaa gtgatttatt taaagaatat cgtgtagatt atgtagttaa
      841 gggcggacaa acaatgaatc cgtcaaccga agacttttta gcagcaatca aaaaggtgca
      901 tgctaagact gttttcatct tacctaataa cggtaacatc attttagcgg caacccaagc
      961 tgctgaagtc actaatgaag aaggtgttgt taatgttcac gttattccaa gtaaaacaat
     1021 cccgcaaggc ttagtcgcag cgatgaactt taaccctgaa gttgatactg aacataacat
     1081 tgaaataatg actgaagcac ttgacgctgt tgaaactgga caaattacct ttgcaattaa
     1141 agatacatca attgatggtt ttgaagttaa aaaagattat tatatgggtt tacaaggtaa
     1201 aaagattctc tgttccttac ctgataaatt tgatgccctt cgcgaactcc ttgctggcat
     1261 gattgatgaa ttaacagcaa ttgtgacgat aatttacggt gaagatgttg aagagagcgc
     1321 cgcagaagaa ttacttgcgg aacttgaaga aaaatattcg gcagttgact ttgaattagt
     1381 aaatggcggc caacctgtat attcgtttat cgtcggtctc gagtagaggt agcgatggcg
     1441 aaaataagta attcatcacg aattattaat gcattagcaa aatatgacat ccacacattt
     1501 agggatgtca tttttcattt accgcgtcgt tatttagata tgtcgtacac tgatgaattt
     1561 aagatgaaca cgaatgaaaa agtgacactt aaattaaaag taacgagtgc accactttta
     1621 attaaaccgc aaagagtggc gattattcgt ttcaccgctg ttagtgaaag tggcgcaact
     1681 tataaaattg ttgcctacaa ccaacgctat atattaaacg ccgtcaaaat aggtgactta
     1741 atcacggtat acggcacaat taatttgcga cttttagaac taacagttat taaactcttt
     1801 ccgcgggtgc taagcgaaga agaacagctg agtccactct atagtttacc aagtgaagta
     1861 ggcaatcatg agtttataag aatcgtgcaa aaagcactta aaatagaaag tgtaacttcg
     1921 cgtgttccgc gccctttagc cgacaaaaac ggtttaattt cgctaaatga tgccttaaaa
     1981 cgtgtgcata atccacgtga cttaaatgat gtctttgaag gacttaaagc acttaaatac
     2041 gaagaaggat tagaatattt tattaagctt ttacttatta aaagggcaaa tagcgaatta
     2101 actaattcaa agaaaacaat tgttaattta aaagacgcaa atgaatttgt taaaagacta
     2161 ccatttaagt taacgaaaga tcaactaact gccgtgcgag aaattggtta cgatatgaat
     2221 aaagaaacac tgatgtatcg tttgcttcag ggtgatgttg ggagcggtaa aacagttgtg
     2281 agttttgtcg cactctatat taattacttg cgtggagccc aaggtgtctt acttgcgcca
     2341 acggaaagtt tagcgaaaca gcattatgaa acgctccttt cattctttaa ggattttgat
     2401 ttacgaatcg aattactaat tggggcttta acgccaagtg ttaaaaccaa attaaaagaa
     2461 gatatccaac atgggttagt cgatattgtc gttggtaccc acgctttatt tagtgaagat
     2521 gtcttttatc ctaaacttga actcgtggtc attgatgaac aacataaatt tggcgttaac
     2581 cagcggaata cactattaaa caaaggcgaa aaggctgatt tactcttact tagcgcgaca
     2641 ccgataccgc gcacacttgc aaatgtaatt tatggcgata tggatatttc aacaattgaa
     2701 agttttccgc atactgggcg acaagtgaat accattatta cgacaagtgc gtctaaaaat
     2761 gtgataccgc ttattgacaa aacactctcg gaaaatagac aagtttatat agtcgcaccg
     2821 aagattagtt tcggtgataa aacaaatgtt ttagcgcttt acgagcgttt ttcagcgcgc
     2881 tacccacatt tagtgggtat gcttcacggc gagcttgcaa gcgaagaaaa agacgcggcg
     2941 ctcttaaact ttaaaagtgg tgtaacacca atcttagtgt caacgacagt cgttgaagtc
     3001 gggattgatg tgaagagtgc gggacttatg ataatttacg aaccacatag ttttggactt
     3061 agttcattgc atcaacttcg aggcagaatt ggccgcgacg ggaaaagggc aacactttgt
     3121 ttagtgagtg aggaagaaga taattataaa cttgaagagt ttgcaagaga agataacggc
     3181 ttcaaaatag cagaacttga tttagcactc cgcgggcctg gtgaattaac gggagagaag
     3241 caaagcggga tgcctagctt tatgtatctt aatgttatta aggatttaaa actaattact
     3301 atcattaaag aagatgcgtt ggaagttatt aataacccga caaatccgca ttattttccg
     3361 cttttaaaag taattaatga tgctttagaa aaagactaat ataaatatct gtctaatatt
     3421 tttgcaggaa catacacttt taatcaatag gaattatagt tactttttca ttgttttaag
     3481 tcacaaaata cgaaattgtc gtctcattat aaaggttatt tttaatttga ctttcaagat
     3541 ttaaaaaatt gaaagagttg aaagtgcaga gctttaaacg gcttagttta tataaaaagg
     3601 tgattatggt aaaataactt tggttatttt taataaggag ataacgacta tgaaacttgt
     3661 aattgaaatg atgggtggcg attacggatt tgaaagcaca atcccagcgc ttatccagtt
     3721 taaaaaagat tatccagaag ttgaactttt tgctgtgggt cgcaaagaag atttagcgcc
     3781 agttgcggat tacgcaacga ttgttgatgc gcgtgatgtt gttaaaattg atgcgacacc
     3841 gcttgaagtg ttacgcgcaa aagaaagttc gcttttaaaa gcaattaata cggtaataga
     3901 agaaaaggct gatgcgctta ttagtgcagg cggaacaggc ccatatttaa gtgcggcaac
     3961 gcttaaactt aaattaattg aaggtgttga acgggccgcc attttagcgc ccttcccaac
     4021 gaaaataaaa ggcaaacaag tcgcaattct tgatattggg gcaagtaacg aaaatactgc
     4081 gagtcactta tatcaatttg ccctaatggg acaacaatac gctagagcgg tatttggagt
     4141 agaagcacca aaaacttatt tattaagtaa cggaactgaa gacaccaaag gttcaccttt
     4201 agtgcttgaa gcttttaaac tttataaaga aaaagaatta ccttacttta tgggtcaaat
     4261 ggaagcacga gacgcgttaa atggcgaggc tgatgttgtg atagcagacg gctttactgg
     4321 aaatgtgtta cttaaatcaa ttgaaggaac cgcaaaagtc ttaatggaca gtgtaaaagg
     4381 cgcttttaaa cgaaacttat tttctaaatt aggatatctc tttgcaagaa aaggtttcaa
     4441 aaacatggca gaaacctttg attataaaaa tacaggtgga gcgattttaa ttggtgttaa
     4501 taatgttgtc gttaaagcac atggtaatag tgatccacgc ggcttttata gcgctattct
     4561 tgttggttac aaaatggcca aagctgatat tgtgaataaa gtacgtgaag ggttaaaaaa
     4621 tgtcgaaact aaatagtctt tttactaaat tagggattac gccaagtaat gagggactct
     4681 ttgaagaagc gttaacgcat tctagttata aaacaaaaga caaagacgcg catgactatg
     4741 aaaggcttga gtttttaggt gacgctattt taaatatgct gattgctagt gcgatttata
     4801 aaagtcatcc tgataaaaaa caaggtgaat taacgaatat gcggaaaaac tttgtgcgca
     4861 cacaaacgct agctacttta ggacgcaaat tagaattaga acgctatatt agaactggta
     4921 aatcaattac accgcaagac ttatatagta acgataaata ttttgaagac gtttttgagg
     4981 cattaatcgg tgcgatgtat cttgatcaag ggctaaaagt aacggaagac tttattttaa
     5041 gaatgttagg cagtgcgatt gagcaatatg actttaatca aatttttgat tacattagta
     5101 cgctacaaga attcatgcag gctgataaaa ggggattacc acaatacatc actgaacctt
     5161 cgggttacga taataaagaa gaagccttta tttcaacagt gacatatgac gggattattc
     5221 ttgggtgtgg ctacggcgca aatagtaaag aagccgaaca aaatgcagca aagaatgctt
     5281 tagaaaaggt ggcgcggtaa gatgggatta atttcatttt taaaaaagaa gtttggtggg
     5341 aagaaaaacg aaaccaccga taaatatgtt gtcggtctag aaaaaagtaa ggcgaacttt
     5401 tctagtaaac taaaacgctt agcaaaacgc tatgaaaaag taacggaaga atattatgaa
     5461 cacttacttg aaatcttaat tggcgcagat gttggtgtta ctttaactta tgaaattatt
     5521 gaagcaacaa aagaagaagc actgcttaat aatttaactg accctgaagc tattaatgac
     5581 cttttagtcg ataagatgtt tattggttac ttaaagcagg gggaagacat agttaacgaa
     5641 attcagtttg cagacaatgg tccaaccgtg ttacttgtgg ttggtgttaa cggcagcggg
     5701 aaaacaacat caattgctaa actagcgcat cgctataaaa aagaaggaaa aaaagtttta
     5761 ttagttgcag gcgatacctt ccgcgctggt gcggttgcac aacttgaagt atgggcaaag
     5821 cgccttgatg tcccgattgt gacaggaaaa ccaaacgctg atcccgcaag tgttgcttat
     5881 gatggagtcg cagaagggaa gaaaattaac gctgatttaa ttattattga tactgcgggt
     5941 cgcttacaaa ctaaggtgaa cttaatggca gaattaagca agattcgccg ggttattggt
     6001 gaccaaatag aaaatgctcc gcatgaagta tttttgatta ttgatgcgac aactggacaa
     6061 aacggagtgc tgcaagctaa agcatttgcc gaagcaactg ggcttactgg tgttgttatt
     6121 acaaagatgg atggcactag taagggcgga ataattttag cgattagaga tgaaattggt
     6181 gtgccagtac gctttattgg cttaggcgaa aaaatggaag acttagatga atttgattta
     6241 gaaaaatatt tatatggctt atgcctaggg gaggacgaat aatggttgtt atttccttac
     6301 ttagtttagt agtgtctttt cttgtgttta tgggagtgcg ccttgcggta tattcgctta
     6361 atccttcaaa ctatttgttc gcggatattt taattattat cattggtagt tttattgtta
     6421 gtcttatctt tgccattcgc caatttagat atatgggaag agataaatgg cgctcacaat
     6481 tctttcacgg ttttgtgctt cgtacattct taattaattt tgcgattatc tttataattt
     6541 cgttactctg gtcttggtga taaaaatgga tgaattaaaa gagtatatgt atattaatga
     6601 gttattacta atttatggta aaacactcac aaaacgacaa ctaaagatta tggaaagcta
     6661 ttacaaatat aatcttggtg tccaagaaat tgcagaagaa ttaaacatca ctaaagcagc
     6721 ggtgagcgat gcgattaaag tatccactaa acacttagaa aatttagaaa gtattgtggg
     6781 gcattacgct tatcgccaac aagtggaagc actgcacgaa gaaataaaaa acacttcaac
     6841 attacaagaa gttaaagaac tcttaagtaa ggaggaagat gatggcattt gaatcattaa
     6901 ccgaacgatt tagtaaaata ttaaaaaaag tccgcggtca atcgcgtctt accgaaaaaa
     6961 atatggacga gatgctccaa gaagtgcgga ttgccctttt tgaagcggat gttaatttta
     7021 aagttgtaaa agaattcgtt aataatatta aagaaaaagc actaggcacg aaagtttatg
     7081 acacacttaa ccctggtcaa atggtcgtaa aaattgttaa cgatgaatta gtggaacttt
     7141 tagggagcac tgatactgaa ctaatttttg aaaaaaataa accaacaatt attatgcttg
     7201 ttggtcttca aggaactggt aaaacaacga ccgctggtaa attagcgctg cattttaaaa
     7261 agaaaatgaa taaaagagtg ttacttgctg cagcagatgt ctatcgacca gcggcaattg
     7321 atcaacttga tacactagcg aaacaagtcg gtgttgatat tgttaattta ggtgataaag
     7381 ttagtcctgt tgaaattgcg aaagccgcga agaaaaaagc atatgacgat cgatacgatg
     7441 tcttaattat tgacactgcg ggacgcttaa caattgatga aacattaatg aatgaattaa
     7501 aagagatgaa agcagcagtg acaccaacgg aaattttatt acttgttgac gccatgagcg
     7561 gacaagacgc ggtaaatact gctttatcct ttaatgagaa gttagggctt acgggcgcta
     7621 ttatgtctaa acttgatagc gacgctcgtg gtggggcggc tttatcaatt aaacatttaa
     7681 ctggtgtacc aattaaattc gctggtgttg gtgaaacgct agaagactta gatattttct
     7741 atcctgaccg gatggcggac cgtatccttg ggatggggga cgttttaacg ctcgttgaaa
     7801 aagcacagca acaacttgat gaaaagacag tgaaaaaaac aacgcgcaag attatggaag
     7861 gcgagtttga tttaaatgat atgctcgaac aaatgaaaca agtacaaaaa ttaggaaaac
     7921 tcggggggat tttaaaatta atcccaggca tgcctaaaat tactcctgaa caacaagaac
     7981 ttgccgaacg cgaaatgaag ctctttgaag ttattattaa ctcaatgacg ccagaagaaa
     8041 gaagtaaccc cgctattttg cggaatagtc gtaaagtaag aatcgctaaa ggcagtggca
     8101 cgagtaatca agagattaat aaagtgatta agaaatatga acaaatgaaa caaatgatga
     8161 aacaaatgaa gggatataaa aaaggcggac caatgccacc gggattccct ggaatgggaa
     8221 ggtaaaaaaa gaacacaaat actgacacca atagaggtga ttgaagttaa cgttcaatta
     8281 gaatatgcag aagaacttat cgtggaaaaa gatacattta cgattaaaat ttagttttaa
     8341 caatggaaat ttgtgaaaaa atgaataatt gtatttaatg atgtaatggc ctttgctgtc
     8401 attaacgcat cctttacaat atgtgcaata tttttttgct gaaacaaaaa gtcttgattt
     8461 aatttattaa tagttaaatc ctttgattag ttcataatac tagcgattgt tattgtggga
     8521 ggacatctac aataaaagtc tttactgcca aatatacttc ttcaaatgtc ggcttactca
     8581 ctccacctgc aaaacgaaac ttttcgtaag aatgttttaa atcttttgtt cggtttagaa
     8641 atccatcaaa t
//
LOCUS       DAOPCD010000017         8486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_7774_length_8486_cov_4.894556, whole genome shotgun sequence.
ACCESSION   DAOPCD010000017 DAOPCD010000000
VERSION     DAOPCD010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7774_length_8486_cov_4.894556"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /gene="rlmD"
                     /locus_tag="PLQ55_01070"
     CDS             complement(<1..1053)
                     /gene="rlmD"
                     /locus_tag="PLQ55_01070"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPK28562.1"
                     /translation="MIKENDIYVVTGGDYTSEGIGLAKVDNFPIFINNLIIGEEAEIK
                     ITKVMRNFATGKIIRFTKMSSDRITPVVPETIHLAGCQFTQLDYAKEVEYKLSKVERA
                     LRVIGGIDFKIEKIHTAKNPYFYRNKTVLPFGKRKSGKVYTGLFRHNTHEIIAMEKTH
                     LDDELASIVIAEVRKLIEKYNYEVYDEKSHTGQVRKVMVRVSHYLREVMVVLITKDEQ
                     IPNLDAFCRDLVAICPNITTIIQNINSEDTNVILGKKEKVLYGAGFINEELHGLKFTL
                     SSKSFFQVNTLQAEVLYERALTLAGITKNDVVLDAYSGTGTIAILAAKKAKQVIGVEI
                     VKDAVKDAEVNAKQNNI"
     gene            complement(1053..2633)
                     /gene="metG"
                     /locus_tag="PLQ55_01075"
     CDS             complement(1053..2633)
                     /gene="metG"
                     /locus_tag="PLQ55_01075"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPK28563.1"
                     /translation="MKKRFYITTPIYYPSAKLHIGHAYCTTLSDILARYNRMIGKEVY
                     FLTGSDEHGQKIAENAAKANKTPQKFVDDIVKTFKDLWEKLAITNDDFIRTTDPKHIA
                     TVQKIFTKLLEQGDIYLGSYEGWYCTPCESFWTTTQVGEEKLCPDCNRSVHLASEESY
                     FFKTDKYVEGLLKKYEETPNFITPESRKNEMVNTFIKPGLSELAVSRTSIEWGVPVRE
                     NERHTMYVWVDALINYISALGYLSEDDELFHKFWQSEDVEIVQILGADITRFHTIYWP
                     MILKALGLRQADRYFVHGLLMMKDGKMSKSKGNVIDPLPLIDKYGVDAVRYYLAREVV
                     FGNDGQFTPEQFVERFNVDLVNDFGNLLNRTVSMIEKYCDGVIPLYRGQVTEFDTEIE
                     DLAINVVKEYHEHFSDLKVTEAITTAFKIISASNKYIDNTKPWELNTQGKKEELDSVM
                     NHLANNLFIGAILLQPVLVNAPKELFKQLGVPQDKQDINLLNKYGVLGGEKVNKGEPL
                     FPRLKVNEEVEYIKNLMK"
     gene            complement(2694..2897)
                     /gene="rpmE"
                     /locus_tag="PLQ55_01080"
     CDS             complement(2694..2897)
                     /gene="rpmE"
                     /locus_tag="PLQ55_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPK28564.1"
                     /translation="MKQGIHPKTQKITVTCVSCGTEFEALSTKKEIRVDTCSNCHPFY
                     TGKQRFTQADGRVDKFNKKYGLK"
     gene            3713..5107
                     /locus_tag="PLQ55_01085"
     CDS             3713..5107
                     /locus_tag="PLQ55_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPK28565.1"
                     /translation="MITKKLALEVLNVALSTGGDFAEIFLEETYSGNYTVENGKTQAP
                     ATSLTYGAGIRILKGLQSVYGHTSDLSKKGLLNLATALSAGFDGQRVLSVEKLKNVRV
                     RKNHELEKSLELVDINEIVEKLKITNKVMKDYDEKIVRAISVLLTDVRNITIFNSEGN
                     AFTDKQERVRLVFQAIAMAPGAFEYSYNGPGAHKGFEYVEGLDLEKIARDVADVAVRK
                     LTAAECPSGKMPVVIGNGFGGVIFHEACGHPLEASSVSKGLSPFAGKVGKQIAHPAVT
                     AVDDGTIVNGWGSNNIDDEGVKTKKNVLIKNGILQSYLVDRFNGRRMGIESNGTGRRQ
                     NYTFEPTSRMSNTYIAAGKYTPEEIISSVKLGLYAKSLGGGQVNPATGDFNFACDEAY
                     IIRDGKVAELVKGASLVGNGADILMKIDMVANDLERAQGMCGADSGSVPVDVGQPTIR
                     VSEITVGGRGGALQ"
     gene            5104..6423
                     /locus_tag="PLQ55_01090"
     CDS             5104..6423
                     /locus_tag="PLQ55_01090"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HPK28566.1"
                     /translation="MNVKKFFELAKEKGLEAAELVIQKNKGIEMSVYDESVEKYTLAS
                     TINVLARGIYKGKMGFAMSEKDDKTTAQYLVAQIKMSAGVSETEDLSIIFEGSPKYRK
                     KSFFNKELATISESTKLESLFTIEKALKKAHPLITSVSTVQYSEIESDYKLFNSYGLK
                     LTNKQNYFYYGTWVVAKEGDDVKSHVEVFLDNDYSKFDADTFVKKAADGVIEKLGGKP
                     CATKDYPVVFNPKMTASLLAAYLTSASADRVEKNTSLFADKLNERVASSKVTVLDAPL
                     TANLFYRYFDDEGVAKTNKPIIKNGILQTFFHNLSTATKMGVEPTGHGRNTGGRISVG
                     ISNITLKPGRNDFDTLIKDIKEGVFITELMGLHSGLNTTSGDFSLQAAGFMIRDGKVA
                     EPVNLITVAGNLVKLFNDVKAVANENELQLSSYTTSSILVKSLKIAG"
     gene            6546..7460
                     /locus_tag="PLQ55_01095"
     CDS             6546..7460
                     /locus_tag="PLQ55_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HPK28567.1"
                     /translation="MKKKLLLLLPFLLLAGCKAREKPDIVVTSFVSYDAVTNIVGDKL
                     KVKNIVPWGSELHDFEPTPRDIEDINNAKLFVYTHPALETWVKDLVTNKNSFDMSIHS
                     DVEYACKVKKQEHHDHEHDHDHTLHYWTDPHIYIRVMNDLLTVLIDISPANETYFTEN
                     HNNYTARLLATSNDFRDFLFPPLEKPPLFFAGHNALGYFAAAYKLTIKTISESYKPDV
                     DFLSQEIIDFMYEIKTFDSHYLFIEELVEPRVANLIKNELAKENYAITLLELHGYHNI
                     TAAQAKEGITYADIFAQNVANIKQAFGG"
     gene            7405..8112
                     /locus_tag="PLQ55_01100"
     CDS             7405..8112
                     /locus_tag="PLQ55_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPK28568.1"
                     /translation="MPTSSRKMSQTSNKPLAVKLTDVSVNFGDVIALKNINFSINKGM
                     LLYVIGPNGSGKTTFIRLLAKLLEPTHGTIEHSDDRLGYLPQKLNINPNFPITVEEVI
                     YSGLAKQSLLISKGDRELIKSWLKKMQIPHLLKKPMAHLSGGQQQRVYIIRALISNPD
                     FIILDEPTSALDPSFRSFFNDIITALHHEGKTIIFVTHELHGALCDCASVLYIDQEIR
                     FFGGLQEYFEKEKNIHV"
     gene            8105..>8486
                     /locus_tag="PLQ55_01105"
     CDS             8105..>8486
                     /locus_tag="PLQ55_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPK28569.1"
                     /translation="MFSFLQHTFMVYALLLALLIALAAALLSPFLVLSKQSLIADGMA
                     HIAFTGVVIGLLFSNEPLYIALPFALLASIAITFLTERANLDNDAAIGVVSVFALALG
                     LIIISKSDGFNRSVESLLVGSLLTV"
BASE COUNT         2635 a         1497 c         1488 g         2866 t
ORIGIN      
        1 aatattattt tgtttagcgt ttacttctgc gtctttaaca gcgtctttaa caatttcaac
       61 accgataact tgtttagctt tttttgctgc taaaatagca attgtgcctg tgccagaata
      121 agcatctaac accacatcat tttttgttat gcccgctaga gttagtgctc tttcgtaaag
      181 cacctcagcc tggagtgtat taacttggaa aaacgattta cttgaaagcg taaatttaag
      241 tccgtgtagc tcttcattaa taaatccagc tccatatagc actttttcct ttttacctaa
      301 aataacattg gtatcttcgc tattgatatt ttgaataatc gttgtaatat ttgggcaaat
      361 tgccactaaa tcacggcaaa atgcgtctag atttggaatt tgctcatctt ttgtaataag
      421 tacaaccatt acttcacgaa gataatgact tacacgaacc attacttttc taacttgccc
      481 agtatgactt ttttcgtcat aaacttcgta attatatttc tcaattaatt tacgaacttc
      541 agcaataact atgcttgcta attcgtcatc aagatgtgtt ttctccatcg caattatttc
      601 gtgcgtgtta tgtctaaata aaccggtata aactttgccg ctctttcttt tgccaaacgg
      661 taatactgtt ttattacgat agaaatatgg gttcttagca gtgtggatct tttcaatttt
      721 aaaatcaatg ccaccgataa cgcgtaaggc tcgttcaact tttgaaagtt tatattccac
      781 ttcttttgcg taatcaagtt gggtaaattg acaacctgca agatggattg tttccgggac
      841 aactggtgta atacgatcac tagacatttt agtaaaacga attattttac cagtcgcaaa
      901 attacgcatt accttcgtta ttttaatttc cgcttcttca ccaataatta agttattaat
      961 gaaaatagga aaattatcga ctttagctag gccaatccct tcactagtgt agtcgccccc
     1021 agttacgaca taaatgtcat tttctttaat cattatttca ttaagttttt aatgtattcg
     1081 acttcttcat taacttttag gcgtgggaat aatggttcgc ccttattaac tttttctcca
     1141 cctaaaacac catatttatt taatagatta atatcttgtt tatcttgcgg caccccaagt
     1201 tgcttaaata attcttttgg tgcgtttact agtacaggtt gcagtaatat tgcgccaata
     1261 aaaaggttat ttgctaagtg gttcattact gaatctaatt cttctttttt accttgagtg
     1321 tttaattccc acggtttagt gttatcaatg tatttgtttg atgcactaat aattttaaaa
     1381 gcagtggtaa tcgcttctgt cacttttaaa tcactaaagt gttcgtgata ttctttaacg
     1441 acattaatcg ctaagtcttc gatttcggtg tcaaattcag ttacttgacc tctataaagc
     1501 ggaataacac catcacaata cttttcaatc atcgatactg ttctatttaa taaattgccg
     1561 aagtcattta ctaaatcaac attaaagcgt tcgacgaatt gttcaggtgt aaattgtccg
     1621 tcattaccaa acacaacttc tcttgctaag taataccgca cagcgtccac gccatattta
     1681 tcaataagcg gcaacggatc aataacattt ccttttgatt tagacatctt tccgtctttc
     1741 atcatcagta gcccatggac aaaataacgg tctgcttgac gtaaccctag tgctttaaga
     1801 atcattggcc aataaatcgt gtggaagcgc gtgatatcgg cacctaagat ttgcactatt
     1861 tcaacatctt cactttgcca aaatttatgg aagagttcat catcttcact taaataccct
     1921 aatgccgaaa tataattaat taaagcgtca acccacacat acattgtgtg gcgctcattt
     1981 tctctaacag gcactcccca ctcaatactc gtgcgtgaga cggcaagttc gcttagtcct
     2041 ggtttaataa atgtattaac catttcattt ttacgcgatt caggcgtaat gaagtttggc
     2101 gtttcttcat atttctttaa taatccttca acatatttat cggttttaaa gaaatatgac
     2161 tcttcacttg ctaaatgcac gctccgatta caatcgggac aaagtttttc ttcaccaacc
     2221 tgagttgtag tccaaaatga ttcacacgga gtacagtacc acccttcgta actcccaaga
     2281 tatatatcac cttgttctaa aagtttggtg aaaatcttct gcacagttgc aatgtgtttt
     2341 ggatcagtcg tgcgaataaa atcatcattt gttattgcta atttttccca gaggtcttta
     2401 aatgttttta caatatcatc cacgaacttt tgtggcgttt tatttgcttt tgccgcgttt
     2461 tccgctattt tttgcccgtg ttcatcactt ccagttaaga aataaacttc tttaccaatc
     2521 atccgattat agcgcgctaa aatgtcactt aaagttgtac agtaagcgtg accaatatgt
     2581 aacttagcgc ttggataata aatgggggtt gttatgtaaa aacgtttttt catagtgtac
     2641 ctcttatatt tgaaaaaact gccatttgat ttaacagttt ttcagtaatc aatttactta
     2701 agtccgtatt ttttattaaa tttatcaaca cgaccatcag cttgggtaaa gcgttgtttc
     2761 cctgtataaa acggatggca atttgagcaa gtgtcaacac ggatttcttt ttttgttgat
     2821 aatgcttcaa attcagtacc acagctaaca caagtcactg tgattttttg cgtctttggg
     2881 tgaattcctt gtttcataat atctccttcc gcctaggtta actcgattta acctaagtaa
     2941 gcaaaagtaa taatatcatt attacagtta tttgacaacc gaaaatatta ttcctgagtt
     3001 ctaataatct cttttatgat attttatgaa atcaataaaa agttcccaaa acaagtaccc
     3061 tagtaaaatt attacttgca acagatagtt gcattttaat atcatagtaa acaatacaat
     3121 ctttttttga agtgaagtgt accccaaatc tttgataaca aaaaaggggt acacatcaac
     3181 atcaacatca atacaactct tttttcttct tttaaatgaa tgttatgaaa tagtaatttt
     3241 ttgatgtttc ttctatatgg tatgcactgt atcgctgcgc ttcaatcaac tcgttaatta
     3301 taataatgaa attactcaaa gttagatttt ttgaatttga acgattattg caatcaaaaa
     3361 catagaaagc aatgttgctt ttacatgctt tttttgaaaa ttgactttta tcataaactt
     3421 tttttgcttc ataacttaat tcatttatgt ttttttattc ttcattaata tttctcctcc
     3481 tttcgttaag ttacaacaaa accaataatt gttaattttg ttgctcatat aatttagcgt
     3541 gattttttta gtgcttttta aagaaatatt aatctttttt attagtagct gcccttattc
     3601 gttacaattt gaatattaaa ttgttgtatt atctaaaatt ccctaaaaaa gcgcttttcc
     3661 ccatatttcc ttcatatttt tgttatcatt tttaattagg aggaagtaca ttatgattac
     3721 aaaaaaactt gccctcgaag tattaaatgt agcgcttagt accggtggag atttcgctga
     3781 aatattttta gaagaaactt acagcggtaa ctacactgta gaaaacggta aaacgcaagc
     3841 gcctgctact tcgttaactt atggggcagg cattcgtatt ttaaaaggat tacaaagtgt
     3901 ttatggtcac acaagtgact taagcaaaaa aggtttatta aatcttgcca ctgcgttaag
     3961 cgctggcttt gatggtcaaa gagttttaag tgttgaaaaa cttaaaaatg taagggttcg
     4021 taaaaatcac gagttagaaa aaagtcttga attagtggat attaatgaaa ttgttgagaa
     4081 gttaaaaatc actaataaag taatgaaaga ttatgacgaa aagattgtac gcgctatcag
     4141 tgtattatta actgatgttc gtaatatcac tatttttaat agtgaaggta atgcatttac
     4201 tgataagcaa gagcgcgtaa gattggtgtt tcaagcaata gcaatggcac caggcgcctt
     4261 tgaatatagc tacaatggac caggagcaca caaaggcttt gaatatgttg aaggtttaga
     4321 tttagaaaag attgcgcgtg atgttgctga tgttgctgtc cgtaaattaa ccgcagcgga
     4381 atgtccaagc gggaaaatgc cggttgtgat tggtaacggc tttggtggag ttatttttca
     4441 cgaagcatgt ggtcacccgc ttgaagcaag ttctgttagt aagggattat caccttttgc
     4501 cggtaaagtc ggaaagcaaa ttgctcaccc agcggttacc gcagtcgatg acggcacgat
     4561 tgtaaacggc tggggatcaa acaatattga tgacgaaggc gtaaaaacaa agaaaaatgt
     4621 tttaattaaa aacggcattt tacaaagtta tcttgttgat cgttttaatg gacggcggat
     4681 gggaattgaa agcaatggta caggccggcg tcaaaattat acctttgaac caacaagccg
     4741 gatgtcaaat acatacattg ccgctggtaa atatacacct gaagaaatca ttagcagtgt
     4801 aaaacttgga ttatatgcaa aatcacttgg tggtggacaa gttaatccag caactggtga
     4861 ctttaatttt gcgtgtgatg aagcttatat tattcgtgat ggtaaagttg ccgaattagt
     4921 taaaggtgct tcacttgttg gtaatggcgc tgatatatta atgaaaattg atatggtcgc
     4981 taatgacctt gaacgcgctc aaggcatgtg cggtgccgat agcgggagtg ttccagttga
     5041 tgttggccaa ccaacaatta gagttagtga aattaccgtt ggtggtagag gaggggcatt
     5101 acaatgaacg taaagaaatt ctttgaatta gcaaaagaaa aaggccttga agccgctgaa
     5161 ctcgttattc aaaaaaataa aggtattgag atgagtgtgt atgatgaaag tgttgaaaaa
     5221 tacaccttag cgtccacaat taacgttcta gcgcgcggta tatataaagg taaaatgggt
     5281 tttgcaatga gtgaaaaaga cgataaaaca actgcccaat acttagttgc ccaaattaag
     5341 atgagtgccg gagtttcaga aacagaagac ttaagtatta tttttgaagg tagtcctaaa
     5401 tatcgtaaaa aatcattctt taataaagaa cttgccacta ttagtgaaag cacaaaactt
     5461 gaatcgctct ttaccattga aaaagcgtta aaaaaagcgc acccgcttat tacttctgtt
     5521 tcaactgttc aatattccga gattgaaagt gattataaat tatttaattc atatggttta
     5581 aaactcacaa ataaacaaaa ctacttctat tatggaacat gggttgttgc taaagaaggc
     5641 gatgatgtta aatcgcatgt cgaagttttc cttgataatg attatagtaa atttgacgct
     5701 gacacatttg tgaagaaggc ggctgatgga gttatagaaa aacttggtgg taaaccatgt
     5761 gctactaagg attatcccgt tgtatttaac ccaaaaatga ccgctagttt acttgcagct
     5821 tacttaacaa gcgcaagtgc tgatcgtgtt gaaaagaaca cttctttatt tgccgacaaa
     5881 ctaaatgaaa gagtagcaag ttcaaaagta actgttcttg atgcaccgtt aacagctaat
     5941 ctcttctatc gttattttga tgacgagggc gttgctaaaa caaataaacc aattattaaa
     6001 aatggtatct tacaaacatt cttccataac ttaagcaccg ctactaaaat gggtgttgaa
     6061 cctaccggtc acggccgtaa cacgggcgga agaattagtg tcggtattag taatatcacc
     6121 ttaaaacctg ggcgtaacga ctttgatact ttaattaaag atattaaaga aggtgtattt
     6181 atcactgaac ttatgggtct ccactcggga ctcaacacaa caagtggtga ctttagttta
     6241 caagcagcag ggtttatgat tcgtgacggt aaagttgctg aacctgttaa cttaattacc
     6301 gttgctggta acttagttaa actctttaac gatgttaagg cggtcgctaa tgaaaatgaa
     6361 ttacaactta gtagctacac tacttcaagt attcttgtta aatcattaaa aattgctggc
     6421 tagtaataaa gtatctttat ctaaatttaa gaggcgtcct ttgtggcgcc tttgcttttt
     6481 attagcgata acgctaagct gtgtgttata attctttttg cgaatcattc gcaaattgaa
     6541 ggattatgaa aaagaaatta ttattactat taccattctt acttttagcg ggttgtaagg
     6601 cgcgcgaaaa accagatatt gttgttactt cgtttgtaag ttacgatgct gtaactaaca
     6661 ttgttggtga caaactaaaa gttaaaaaca ttgtcccttg gggcagtgaa cttcatgact
     6721 ttgaacctac accacgtgat attgaagata ttaataacgc taagctattt gtctatactc
     6781 atcctgcgct tgaaacatgg gttaaagacc ttgtcactaa taaaaatagt tttgatatga
     6841 gtattcattc tgatgttgaa tatgcttgca aagtaaaaaa acaagaacat cacgatcacg
     6901 aacacgacca tgatcacaca cttcattact ggacagatcc acatatatac atccgtgtta
     6961 tgaatgatct tttaactgtg cttattgaca tttcgccggc aaatgagacc tattttacag
     7021 aaaatcataa taattacaca gcgcgattat tagccacaag taatgatttt cgtgactttt
     7081 tgtttccacc gttagaaaaa ccaccgcttt tctttgcggg gcataatgcg cttggttatt
     7141 ttgctgctgc ttataaacta actattaaga caattagcga aagttataaa ccagatgttg
     7201 attttttatc acaagaaatt attgatttta tgtatgaaat taaaacattt gattcgcatt
     7261 acttattcat cgaagaatta gtggaaccgc gtgtcgctaa tttaattaaa aatgaattag
     7321 caaaggaaaa ttatgcgatt actcttttag aacttcatgg gtatcacaat attactgcag
     7381 cacaagcaaa ggagggtata acatatgccg acatcttcgc gcaaaatgtc gcaaacatca
     7441 aacaagcctt tggcggttaa actcactgat gtaagtgtaa attttggtga tgtcatagcg
     7501 cttaaaaaca tcaacttttc aattaataaa gggatgctcc tttatgtgat tggtcctaac
     7561 ggatcaggta aaacaacttt tataaggttg cttgctaaat tgttagagcc gacgcatggc
     7621 actattgaac atagtgatga tcgtttaggt tatttaccgc aaaaattaaa tattaaccct
     7681 aatttcccaa tcacggttga agaagtaatt tatagcggcc ttgctaaaca gtcactactg
     7741 attagtaaag gtgatcgaga actaataaaa tcatggctta aaaagatgca aatcccgcac
     7801 ttgcttaaaa aaccaatggc ccatctttct ggcggtcaac aacaacgcgt ctatattatt
     7861 cgcgcgctta ttagtaatcc tgattttatt attttagatg aacccacttc tgcactcgac
     7921 ccgtcctttc gcagtttctt taacgacata attacagcat tacatcacga aggaaaaaca
     7981 attatttttg tcacacatga acttcatggt gcgctttgtg actgcgctag tgtgttatat
     8041 attgatcaag aaatacgttt ctttggcggc ttacaagagt attttgaaaa ggagaaaaat
     8101 atccatgttt agtttcttac aacacacttt tatggtttat gctcttttgt tagcgctgct
     8161 tattgcccta gcggccgctc ttttaagccc gtttcttgtt ttatctaaac aatctttaat
     8221 tgccgacggc atggcgcata ttgcctttac cggcgttgtt attggattac tatttagtaa
     8281 tgaacccctt tatattgcct taccttttgc gcttttagcc tcaattgcca ttaccttttt
     8341 aacagagcgc gctaatctag ataatgatgc agcaattggt gttgttagcg tctttgcttt
     8401 agcgcttggt ttaattatta ttagtaaaag cgatggtttt aatcgctctg tggaatcgct
     8461 attagttggt agtttattaa cagtta
//
LOCUS       DAOPCD010000018         8202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_8062_length_8202_cov_4.304529, whole genome shotgun sequence.
ACCESSION   DAOPCD010000018 DAOPCD010000000
VERSION     DAOPCD010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8062_length_8202_cov_4.304529"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1863
                     /gene="mnmG"
                     /locus_tag="PLQ55_01110"
     CDS             <1..1863
                     /gene="mnmG"
                     /locus_tag="PLQ55_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPK28570.1"
                     /translation="IVVGGGHAGVEAALASAHMGKNTLLLTMDIKKIANMPCNPNIGG
                     SAKGIVVREVDALGGMMGIFADHDPLQIKILNTGKGPGVRCLRAQEDKVGYPRYVQSI
                     VLNTPNLTVKEAMVVSVIHDTEKVYGVRLENDEEILAKATILTTGTYMESTILVGHTV
                     KEIGPEGDRSSKGLSPYLEKMGLKIFRLKTGTPARLKKDTIDYSVMVEEPGMEGYLNF
                     SYMSERYHTLEEQVLCHLLYTTPETHAIINEHLGDSAMYSGLVTGTGARYCPSIEDKI
                     VRFSDKERHQLFIEPESRYTDSMYLQGLSTSMPVEVQEKMIRSLKGLENVEILKYGYA
                     IEYDAIDPLELKPTLELKKWDNLYCAGQIIGTSGYEEAAGLGLLAGINAVLKIDNKPP
                     LILKRDEAYIGVMIDDLVTKGTEEPYRLLSSRSEYRLLLRHDNADSRLTEIGRQVGLV
                     KDDRYAKFLKSQKAIADARAILETTQVSADIPVVEFLESVGYPNYSGGLTIAELIKRP
                     RVTLDDLLELLGHLTLEGLNHNDLFQLETDIKYEGYINKQRQEAKRLKKLEEVEIPAS
                     FTYDDLDGLRIEARQRLAKVKPLTIGQAKRISGVNPNDIIILMTYLRRYGKNEK"
     gene            1853..2554
                     /gene="rsmG"
                     /locus_tag="PLQ55_01115"
     CDS             1853..2554
                     /gene="rsmG"
                     /locus_tag="PLQ55_01115"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPK28571.1"
                     /translation="MKNNPFLQYLDEHFSENAEVLKVQFAKYAALIEEHNKVQNLTAI
                     APSEYEEKHFLDCLLLRETYNFRNETLLDIGSGAGFPGVVLAIAYPELQITLLEPIKK
                     RCNFLSTVVKELELTNVEVVNERAEDYAASKRGEYDLVTSRAVSSLSIMLELSVPFLR
                     INGSALMMKGRNYNAELSDAENAIYTLKVEVGKTDLHFLPSDGSMRVNARFIKKGSTP
                     KKYPRPFAQIKKNPL"
     gene            2588..3367
                     /locus_tag="PLQ55_01120"
     CDS             2588..3367
                     /locus_tag="PLQ55_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003323489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPK28572.1"
                     /translation="MAKIIAIANQKGGVGKTTTAINLSASFAHFGRKVLLIDMDPQGN
                     SSRGLGLDPATIKNTIYHTFFKGININKAIYRTPLKSLDIIGANLFLARTDVEISTIP
                     QHPFTVLQSKIKEIDRRYDYIFIDCPPSLGLLSTNALVAANSVIIPVQCEYFALEAVA
                     QVLATIKQVQASHNSELKIEGFLLTMYDERTRLGIEISQQIRGLFKESTFMTPIPRNI
                     ALAEASAKMLPTILYKPTSKGSLAYLNVAKEIMENEERAEE"
     gene            3345..4151
                     /locus_tag="PLQ55_01125"
     CDS             3345..4151
                     /locus_tag="PLQ55_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPK28573.1"
                     /translation="MKNEPKNNLLKEHLSDLISKYSQSNVLEELAKAHTNRPIQMLPI
                     DLIVDNNFIKKITLSRKKIARFVENYQRNILLEPLVVRAKGDKYEVIIGRRRLAAARL
                     TEIQEIPVIVMNYNDEETLLILLAKARDQLGTNVLELAYIFSVLSTKHGYSHQALADL
                     TFRSRSQVTNILRLLKLEPEVIRALNNEQITFGHARALISFDREKSLEYLELIRVNNL
                     SVRELEALIKAPNTATYNGKDYFVIQMKNKLTIVFNTEEEALAFKNKHFK"
     regulatory      4191..4303
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4342..5001
                     /locus_tag="PLQ55_01130"
     CDS             4342..5001
                     /locus_tag="PLQ55_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPK28574.1"
                     /translation="MEKEKNYTNCQNDNLETNAQQKCKRRRTVTVAGTAIFAAISVLL
                     YITKVPPFVFPIFPLVNFLEINFSEIPALIAGFAYGPLSGFLVLLVRFLVKLPMSSTA
                     MVGEVADIIYSSSFVLTATIIYKYRRTFKGALLGLGIAFIAQILVSSLANYFVIYHLY
                     NEMFFNGEMAMTLSNKYGAFTPEQFILWVIPFNIIKNTIIGGLTLVVYKRISRFINQF
                     Y"
     gene            complement(5056..5250)
                     /locus_tag="PLQ55_01135"
     CDS             complement(5056..5250)
                     /locus_tag="PLQ55_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPK28575.1"
                     /translation="MAKKVKLDKDLCISCGVCNAVCPSLFDWDDDGKLKALVEEVPAD
                     LEDAAAEAEASCPTGAITVE"
     gene            5375..5695
                     /locus_tag="PLQ55_01140"
     CDS             5375..5695
                     /locus_tag="PLQ55_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28576.1"
                     /translation="MAKRRESKTRVEYWAKYRESITNDFEVEQKMRESRKKQAENTPI
                     KKTETPPLTKAEALLSEYERKNENKESAVKKGPIGILYGLAIIVLLLIIAGIIFMILI
                     YGNK"
     gene            5682..6356
                     /gene="cmk"
                     /locus_tag="PLQ55_01145"
     CDS             5682..6356
                     /gene="cmk"
                     /locus_tag="PLQ55_01145"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPK28577.1"
                     /translation="MGINNIAIAIDGPAAAGKSTIAKKVANHYNFVYIDTGAMYRAFT
                     WYVLKRGVDPKDETASNSLIPEVTIRLTADYKVFVNGEDVSKAIREPSVSGNVSYIAS
                     YPKIRLFLTEQQREMAKVNSVVMDGRDIGTYVLPDAFVKIFQTASVAVRAKRRYLENL
                     EKGIATSLEDIEKELAMRDHIDSTRAMAPLKKADDAIELDTDNLSIDEVVAEIIKIVD
                     KKLGVK"
     gene            6356..7663
                     /gene="der"
                     /locus_tag="PLQ55_01150"
     CDS             6356..7663
                     /gene="der"
                     /locus_tag="PLQ55_01150"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPK28578.1"
                     /translation="MALPVVAIVGTPNVGKSTVFNRIIGERRAIIHDERGVTRDRLYG
                     SAEWLTRRFNLIDTGGIEIAKTAFQEQIRAQVEIAIEEADVIVYVGDGPLGVTTDDRE
                     IANLLYRANKPVIVAVNKIDGIEHHHYIHDFYSLGLGEPIAISGEHGIGIGDLLDEII
                     KVLPEQKDDYEEDVISFAVIGQPNVGKSSLVNAILNRERVIVTNVEGTTRDAIDTPFT
                     RNNKNYVVIDTAGLKKRGKIYESVDRYAALRAFSAIAKADIILFVIDAVVGIREQDKH
                     VVGYAVDEKKAIIIAVNKWDAITKDDKTMNEWTNKIRNEFKFVEYAPIVFVSALTKQR
                     VDQIFDQIDYVNEAYRRRVETSLLNEVIHRAQLYNDPPVFNGGRLKVYYANQVSTKPP
                     TFVFFVNNPAFLHFSYERYLENQIRDSFPFDGTPLKFIFRERK"
     gene            7820..8092
                     /locus_tag="PLQ55_01155"
     CDS             7820..8092
                     /locus_tag="PLQ55_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPK28579.1"
                     /translation="MNKAEIIAAIADELMITKRSVEEIINLFCDTVIETLAKGEDVKL
                     TGFGTFQTRERKERRGVSPQTGKEIVIPSGRTIVFKPSKLLKEKVQ"
BASE COUNT         2739 a         1324 c         1658 g         2481 t
ORIGIN      
        1 atcgtcgttg gcggtggtca cgctggggtt gaagctgcgc tagctagtgc ccatatgggt
       61 aaaaacaccc tgcttttaac aatggacatt aaaaaaattg ccaatatgcc atgtaatcct
      121 aatattggtg gtagcgctaa agggattgtg gtgcgtgaag ttgacgcgct tggcgggatg
      181 atgggtattt ttgctgatca cgatccacta caaattaaaa ttttaaatac tggtaagggt
      241 cctggtgtcc gttgtttaag agcacaagaa gataaagttg gttatccacg ttatgtccaa
      301 agcattgtgc taaatactcc aaatttaact gtaaaagaag caatggtagt ttcggtcatt
      361 catgacacag aaaaagtgta tggggttcgc ttggaaaacg atgaagaaat attggcaaag
      421 gcaacaatat taacaactgg aacatacatg gaatcaacaa ttttagttgg tcacactgtt
      481 aaagaaattg gtccagaagg agaccgttct agtaaaggtt tatcgccgta tttggagaaa
      541 atggggctta aaatctttag acttaaaact ggaacaccag cgcgacttaa aaaagatacg
      601 attgattata gtgtgatggt tgaagaacca gggatggaag gctacttaaa tttttcctat
      661 atgagtgagc gctatcacac ccttgaagaa caggtgctct gccacttact ctacactaca
      721 cctgaaacgc acgcaattat taatgaacat ttaggtgatt ccgcgatgta tagcgggctt
      781 gtaacaggaa cgggtgctag atattgtcca agcattgaag ataaaattgt gcgctttagt
      841 gataaagaac gtcatcaatt attcatcgaa ccagaatcac gttacactga ttcaatgtat
      901 ttacaaggat taagtacttc aatgccagtt gaagttcaag aaaaaatgat tcgcagtcta
      961 aaaggtttag aaaatgttga aatattaaaa tatggttatg cgattgaata tgatgcaatt
     1021 gatccacttg aacttaaacc aacactagaa cttaaaaagt gggataactt atattgcgct
     1081 ggtcaaatta ttggcacaag tggatacgaa gaagcagcgg gacttggtct tttagcagga
     1141 attaatgctg tgttgaaaat agataataag ccacccttaa ttttgaaaag agatgaggcc
     1201 tacattggtg ttatgattga tgacttagtg acaaaaggca ctgaagagcc atatcgttta
     1261 ttatcaagta gatctgaata ccgcttactc ttaagacatg ataacgctga ttcgcgcctt
     1321 accgaaattg ggcgacaagt tggcttagta aaagatgatc gttatgcgaa gtttttaaaa
     1381 agtcaaaaag ccatagcgga tgctcgagca attttagaaa caacacaagt aagcgcggat
     1441 attcctgtcg ttgaatttct cgaaagtgtt ggttatccta attattcggg cggattaacc
     1501 attgctgaac ttattaaaag accgcgagtt acactagatg atttattaga gcttttagga
     1561 cacttaacac ttgagggtct taaccacaat gatttatttc aactagaaac tgatataaaa
     1621 tatgaaggat atattaataa gcaacgccaa gaagcaaagc gtttaaagaa gcttgaagaa
     1681 gtagaaattc ctgcttcttt cacttatgat gatttagatg gtttgcggat cgaagcgcgg
     1741 cagcgcttag ctaaagttaa accacttaca attggtcaag ctaaacgtat tagtggtgtt
     1801 aatcctaacg atattattat attaatgaca tatttgagga ggtatggtaa aaatgaaaaa
     1861 taacccattt ttgcagtact tagacgagca ttttagcgaa aatgctgaag tgttaaaagt
     1921 gcaatttgca aagtatgctg cacttatcga ggagcataat aaagtacaaa atttaaccgc
     1981 aattgcccca agcgaatatg aagaaaagca ttttttagat tgcttattac ttcgcgaaac
     2041 atataatttc cgcaatgaaa cattattaga tatcggtagt ggtgcagggt ttccaggagt
     2101 tgtgttagcg attgcttatc ctgaattaca aataacttta cttgaaccaa ttaagaagcg
     2161 ttgtaatttc ttaagtactg tcgttaaaga attagaactt acaaatgttg aagttgttaa
     2221 tgaacgtgcg gaagattatg ccgcaagtaa acgcggtgaa tatgatttag tcacaagtcg
     2281 cgctgtgtct tcgctaagta taatgcttga actaagcgta ccgtttttac gcatcaatgg
     2341 cagtgctttg atgatgaagg ggcgaaatta taatgctgaa ttaagtgacg ctgaaaatgc
     2401 aatttacacc cttaaagttg aagttggaaa aacagattta catttcttac caagtgacgg
     2461 tagtatgcgg gtcaacgctc gctttattaa aaagggaagt actcctaaaa aatatccgcg
     2521 tccattcgca caaattaaga aaaatccact ttaaagtata tttgatatac ttattacaag
     2581 gaatagtatg gctaaaatca tcgcaattgc aaatcagaag ggcggtgttg gcaaaacaac
     2641 gaccgcaatt aatttaagtg cctcttttgc acattttggg cgcaaggtac ttttaattga
     2701 tatggacccg caaggcaata gtagccgtgg gcttgggctt gatcctgcga cgattaaaaa
     2761 tacgatttat catacttttt tcaagggcat taatattaat aaagctattt atcgcacacc
     2821 actaaaaagt ttagatatta ttggcgctaa tctttttctg gcgcggactg atgtggaaat
     2881 tagcacgatt ccgcaacacc cgtttaccgt tttacaatca aaaattaaag aaattgatcg
     2941 ccgttatgat tatatattta ttgattgccc gccttcactt ggactattaa gtactaatgc
     3001 tttagtggcg gctaatagtg taattattcc tgtgcaatgt gaatattttg cgcttgaggc
     3061 tgttgcccaa gttttagcga cgattaaaca agttcaagcc tcacataatt cggaactgaa
     3121 aatagaaggt tttctgttaa cgatgtatga cgaaagaact agactaggaa ttgaaattag
     3181 ccaacaaatt agaggtctat ttaaagaatc aacatttatg acaccaatcc cgcggaatat
     3241 cgctctagcg gaagcaagtg ctaaaatgct gccaaccatt ttatataaac cgacttcgaa
     3301 aggttcatta gcctacttaa atgtagctaa ggagattatg gagaatgaag aacgagccga
     3361 agaataattt gttaaaagaa cacctttcag atttaatttc taaatattcg caatcaaatg
     3421 ttttagaaga acttgcgaaa gcacatacga atcggccgat tcaaatgttg ccgattgatt
     3481 taatagtaga caataatttt attaaaaaga ttactttgtc ccgtaaaaaa attgcgcgct
     3541 ttgttgaaaa ttatcagcgt aatattttac tagaaccact cgtggttaga gcaaaaggtg
     3601 ataagtacga agtaattatt ggacgccggc gtctagcggc agcaagactt acagaaatcc
     3661 aagaaattcc cgtcatcgtt atgaattata atgatgaaga aaccttatta attttattgg
     3721 cgaaagcgcg tgatcaactt ggtactaatg ttttagaatt agcgtatatc tttagtgttt
     3781 tatcgacaaa acacggctat tctcaccaag cacttgcgga tttaacattc agaagcaggt
     3841 cgcaggttac aaatatttta cgacttttaa agcttgagcc agaagttatt cgtgcgctta
     3901 ataacgagca aataactttt ggacatgcac gggctttaat ctcatttgat cgcgaaaaaa
     3961 gtcttgaata tttagaatta attcgtgtta ataatctctc tgtccgtgaa ttagaagcct
     4021 taataaaagc accaaatact gcgacatata atggtaagga ttattttgtt attcaaatga
     4081 aaaataaatt aacaattgtg tttaataccg aagaagaagc actagcgttt aaaaacaaac
     4141 attttaaata atcatttatc ttgaatttat taccgataac tactattata aaagtacttc
     4201 agggcggggt gaaagtcccc accggcggta taccccgcga gcgcaagctg aactagtgaa
     4261 attctagtgg cgacagttaa agtctggatg gaagaagttg tttcttttat gtgtagccct
     4321 gaatagagag ggctattttt tatggaaaaa gagaaaaatt acaccaattg tcaaaatgat
     4381 aatttagaaa ctaatgcaca acaaaaatgt aaacgcagaa gaacagttac tgtcgctggt
     4441 acggcaatat ttgccgctat ttcagtctta ctttatatta ctaaagttcc cccgtttgtg
     4501 ttcccaattt tcccgcttgt taatttttta gaaattaatt ttagtgaaat tcctgcactt
     4561 attgccggct ttgcttacgg gcctttaagc ggattccttg tgttattagt gaggtttcta
     4621 gtaaaactac cgatgtcatc aactgcaatg gtcggtgaag tcgctgatat tatatattca
     4681 tcgagctttg tccttactgc cacaatcata tataaatatc ggcggacttt caaaggggcg
     4741 cttcttggtt taggcatcgc ctttattgcg caaattttag ttagcagtct tgccaattac
     4801 ttcgttattt atcatcttta caatgaaatg ttttttaacg gtgaaatggc aatgacttta
     4861 agtaataaat atggggcttt tacaccagag caatttattt tatgggtcat cccttttaat
     4921 attattaaaa atacaattat cggcggacta acactcgttg tatataaacg aattagtcgc
     4981 tttataaatc agttttatta gcataaaaaa accagcaccc gaagtggctg ctggtatttt
     5041 tttaattctc ttaacttatt cgacggtgat tgcaccagtt ggacacgaag cttcggcttc
     5101 agctgcagca tcttctaaat ctgctggaac ttcttcaacg agagctttga gttttccgtc
     5161 atcatcccaa tcgaaaaggg aaggacaaac tgcgttacaa acgccacaac taatacataa
     5221 atctttatcg agtttaactt tttttgccat aatttcctcc tgatgaataa tattataact
     5281 atttgagagt atcaattcaa atcatattgg aaacgataat tataaaattg tcgtgtaagc
     5341 attttatgtt tatttatatt attataaata cagtatggca aagcgtagag aaagtaaaac
     5401 gagagtagag tactgggcaa aatatcgtga gagtattacg aatgattttg aagttgaaca
     5461 aaaaatgcgt gaatcacgga aaaaacaagc tgaaaatact cctattaaaa agactgaaac
     5521 accaccgctg acaaaggctg aagctttact tagtgaatat gaaaggaaaa atgaaaataa
     5581 agaaagcgca gtaaaaaaag gaccaatagg catattatat gggctagcta ttatcgtatt
     5641 gctccttatt attgctggta ttattttcat gattttaata tatgggaata aataatattg
     5701 ctatcgctat tgatggacct gccgcagcag gaaaaagtac aattgctaaa aaagtagcga
     5761 atcattacaa ttttgtctat atcgatactg gtgctatgta tcgcgctttt acgtggtacg
     5821 tattaaaacg cggtgttgat ccaaaagatg aaacggcatc aaatagttta attcctgaag
     5881 taacgataag gttaactgct gattataaag tttttgttaa tggtgaagat gtaagtaaag
     5941 caattcgtga acctagtgtt tcaggcaatg taagctacat tgcttcatat cccaaaattc
     6001 gtttattttt aactgaacaa caacgggaaa tggctaaagt taatagtgtc gtaatggatg
     6061 gccgtgatat tggtacatat gttttaccag acgcttttgt aaaaatattt caaaccgctt
     6121 ctgttgcagt gcgggcgaaa agacgatact tagaaaattt agaaaaaggg attgccacta
     6181 gtttagaaga tattgaaaaa gaactagcaa tgcgtgatca tattgattca acaagagcaa
     6241 tggcgccact taaaaaggcg gatgacgcta ttgagttaga tactgacaac ttatcaattg
     6301 atgaagttgt cgctgaaatt atcaagatcg ttgataagaa attaggagtt aaataatggc
     6361 attaccagtt gtggcaattg ttggaacacc aaacgttggt aaatcaacgg tttttaatcg
     6421 tatcatcggt gaaagacgag cgataattca tgatgaacgc ggggtgaccc gtgaccggct
     6481 ctatggaagt gctgagtggt taacgcgtcg ctttaatctt attgatactg gcggaattga
     6541 aattgctaag acagcattcc aggaacaaat tagagcgcaa gttgaaattg cgattgaaga
     6601 agcggatgtt attgtttatg ttggggacgg tccgcttggt gttacaaccg acgaccgtga
     6661 aattgctaat ttactatatc gcgctaataa gccagttatc gttgcggtta ataaaattga
     6721 cggaattgaa catcatcatt acatccatga cttttatagt ttaggcttag gtgagccaat
     6781 cgctatcagt ggtgaacacg ggattgggat tggggactta cttgatgaaa ttattaaagt
     6841 attaccggaa caaaaagatg attatgaaga ggatgtaatt tcttttgccg ttattggcca
     6901 acctaatgtc ggaaaatcaa gtttagtaaa cgcgatacta aatagggaac gcgtcattgt
     6961 tacaaatgtt gaagggacaa cgcgtgacgc gattgatacg ccttttacta gaaacaataa
     7021 aaattatgta gtaattgaca ctgctgggct aaaaaagcgt ggcaaaattt atgaaagtgt
     7081 cgatagatat gcagcgcttc gggctttcag cgcgattgct aaagctgata ttattttatt
     7141 tgtcattgac gctgttgtgg gtattcgcga acaagataaa cacgttgttg gttatgcagt
     7201 tgatgaaaaa aaggccatta ttattgctgt taataaatgg gacgctatta ctaaagacga
     7261 caaaacaatg aatgaatgga caaataaaat tagaaatgaa tttaaatttg ttgaatatgc
     7321 tccaattgtt tttgttagtg ctttaactaa acaacgagta gatcaaatct ttgaccaaat
     7381 tgactatgtt aatgaagcgt atcgtagacg tgtagagaca agtttactaa atgaagttat
     7441 ccatcgcgct caattgtata atgacccgcc agtgtttaat ggcggtcgct taaaagtgta
     7501 ctacgctaat caagtaagca ctaaaccacc gacatttgtg ttctttgtga ataaccctgc
     7561 ttttctacat ttctcttatg agcgttactt agaaaatcaa attcgtgact cgttcccgtt
     7621 cgatggtaca ccactgaaat ttatattccg tgagcgtaaa tgagattaaa catttgttca
     7681 tttttagggt tgttttaagt gtaaaaaaat caataaagtc gtaaggcatc gcaataattt
     7741 tcaataaaat attaaatatt tcttgtataa attatataat tatgctactt tgtaaaaaac
     7801 aaaggagtga gaaaataata tgaacaaagc agaaataatt gctgccattg ccgatgagct
     7861 tatgattact aagcgttctg tggaagaaat tattaattta ttttgtgaca cagtcatcga
     7921 aaccttagcg aaaggtgaag atgtaaaact tacaggtttt ggcactttcc aaacaagaga
     7981 acgtaaagaa cgtcgtggcg ttagcccaca aaccggtaag gaaattgtta ttccaagcgg
     8041 ccgtactatc gttttcaaac cttcaaaact tctcaaagaa aaagttcaat aaataactct
     8101 ttgctagaga agtatgttga aatcatattt agagtttttt gacgagattg ctaatatttt
     8161 caacaagcac ggctttaaac tctacatggt tggagggaca ag
//
LOCUS       DAOPCD010000019         8132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_8129_length_8132_cov_4.452148, whole genome shotgun sequence.
ACCESSION   DAOPCD010000019 DAOPCD010000000
VERSION     DAOPCD010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8129_length_8132_cov_4.452148"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLQ55_01160"
     CDS             <1..218
                     /locus_tag="PLQ55_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28580.1"
                     /translation="TEAKEEGEAAPEVAEKPAAAAKPKKEKEVKEPKAEKAEEPKVKA
                     EPKAKAPKAPKVEEVKTEEVKTEKEGE"
     gene            222..1082
                     /gene="tsf"
                     /locus_tag="PLQ55_01165"
     CDS             222..1082
                     /gene="tsf"
                     /locus_tag="PLQ55_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013489060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPK28581.1"
                     /translation="MASLIDKIKELRELTGAGMMDCKKALVENGEDVDKAKDWLREQG
                     ITTAAKKSGRIAAEGLALAKIEDGYAAIVEVNCETDFVSRGDDFKALVNTVLDLLLKH
                     KPANKEAADAIINPLLVEATVKIGEKLSFRRFELVKVEDRGATYIHMGGKIATVVLLD
                     KKNDELAHGLTMHIAANSPSYITTDDITEEDYNHERGIQLEITKNDPRLADKPEKMLK
                     NIVEGKIRKYFSDLVLAEQTYLMDDTKKVGQALKDLDNKVLVMFRYQVGEGIEKRQED
                     FAAEVFGQIK"
     gene            1133..1852
                     /gene="pyrH"
                     /locus_tag="PLQ55_01170"
     CDS             1133..1852
                     /gene="pyrH"
                     /locus_tag="PLQ55_01170"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPK28582.1"
                     /translation="MVYKRVLLKLSGEALAGDKKSGIYDPKVLSAILNFVKSFRHHQK
                     WEIGIVVGAGNIWRGKNAELIGFDPAAADYMGMTATFLNSLALKDYFTQNDLPAVVLN
                     AFAVEGLAEKTSPELAKKHLAKGEVVIFGGGTGKPFYTTDTGAALRAIDMNADLIIFV
                     KDGVDAVYTKNPHQYKDAQKITQVTYQELIDLKVEAMDLSALEVLNKTNITSFVIGMD
                     EVLNFEPLEEHMEIGTIITKR"
     gene            1858..2403
                     /gene="frr"
                     /locus_tag="PLQ55_01175"
     CDS             1858..2403
                     /gene="frr"
                     /locus_tag="PLQ55_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPK28583.1"
                     /translation="MDEYALLAEERMEKSILNLQSEFSTLRTGRASAALLDRIECDYY
                     GEKMPINQISSISVPEPRQLLIKPYDKGDMRSILAAINASTLGINPINDGDTIRLILP
                     PLTEERRRDLVKQAKKFTEEAKIAIRNIRRDANTSIKNDKDLSEDMSKRAEEAVQKVT
                     DEYVKKADDLLKEKEEEILTV"
     gene            2766..3485
                     /gene="uppS"
                     /locus_tag="PLQ55_01180"
     CDS             2766..3485
                     /gene="uppS"
                     /locus_tag="PLQ55_01180"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HPK28584.1"
                     /translation="MSKTKITNYENYGIKHIAFILDGNGRWAKARKRPRTYGHEKGFS
                     SLFDVGIAVSKRNIPFMSVYAFSTENWSRPKKEVDFLFRTLEKRLHSVLKKVQKHNIK
                     IVTSGDLKRLPESTQQAINYAKEESKNNTGTTLNICINYGGKDEIVRAIKRLAADESV
                     NIQALTPDTFENYLDTSGMPPVDLLVRTSGEMRLSNYMLWQLAYAEFIFTPTYWPDYD
                     EECLVRDLEEYAKRTRRFGGL"
     gene            3488..4540
                     /locus_tag="PLQ55_01185"
     CDS             3488..4540
                     /locus_tag="PLQ55_01185"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPK28585.1"
                     /translation="METNEIKPKTKRTSTIKISLNEISEATKKSMKTRTITSIILAVI
                     AVPALLLGGWYFLALVFFVSIIAVLEIMRGTKISKRYWCVYILAIVSTVALIFWIFIK
                     NNIMANIKAGYSWWDISKWVVENGFESIQVSSFLLATILIGLFILIVFDPHLGFDIAA
                     YLFLLVLFAEFGFQAFLFLRLYPQYIADRPDITFNTNIITSAFLIIYVILGTIMNDIG
                     AYFIGVLFGKNKMVPRISPNKTWEGFAGGVFFSIVVSFLFALIVSWIGYPVLPFLSHK
                     EWYYLLLISILMPLAANIGDLFFSATKRHLAIKDYGSILVGHGGILDRVDSLLMVSIV
                     TAMLIILINNGWSILV"
     gene            4553..5809
                     /locus_tag="PLQ55_01190"
     CDS             4553..5809
                     /locus_tag="PLQ55_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPK28586.1"
                     /translation="MEFLSTILNIILFLVMLGVLITIHELGHFIAAKIFRVYVFEFAI
                     GFGPKIFRKKKGETYYSLRALPLGGYVAMLGEEDAVPDEFVNEEIPKERSLIGINRGK
                     RALIMSAGIIMNLVLGYAIFLFANGVLPQTGLSIRLEVKEDSPVYAMGIRSDDRIDFK
                     ETKIDGYTIQYFGEGKLDAAALEPEYYVLFQPKSLDDLTFGGESLVLLKIGATDLASE
                     NSFYTDLKDTDVLTFTATFLRGETYETAERIEKVLTLSTEPKNPDKLTDGYKFVSAGF
                     NLTKFKYRNNFSETFTAANKDFIRSVTAVARGLKSLFSDGLKNVSGPVGIFNISSQTL
                     KNHGLAPYLFLWGLISINLALFNLLPFPPLDGWHLLVVTVEAITRQEISPKFKQIASM
                     IGAILLIGLTLVILAKDIFGFVGVIL"
     gene            5810..>8132
                     /locus_tag="PLQ55_01195"
     CDS             5810..>8132
                     /locus_tag="PLQ55_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPK28587.1"
                     /translation="MGNVSARFFASINYENDKKIGLTFLEWKKVDEVWHYTLLKEKPW
                     NFEDYLDFSDALMSIPYAYKVSYAYTKLPRIEDALALFKPWHLHTYLSESNYVIMVVG
                     NDTLRFTFTDEVSALDAKAVLYEFSLLLKELSYNFALETVVISSAQEEDEEDEFSLGS
                     ERPFIEDDDDDEEYFDEEDDTGVADCDPLDPNENCERSLEYHDGLEDKLKENYEKMVK
                     ERQKRRHAKGAFNLTEIKDLEHKEVDEGVDFDGEIYSYDRRPTKHGVTYTLGIGKDAH
                     GISVRFYINKRGAKQDEVLKALKTGRFVRVQGYIAIDNFRGQTKIVRGRDIDEIAAPP
                     LPTDDYEGRKRIELHLHTKMSALDAVSTIDEYCELAKNMGHEAIALTDHGVVQAFPEA
                     QKAGKKYDIKIIYGSELYVIDETLKVVNNPAPIILNNARYVIFDLETTGLSARYNEII
                     EFGAVRVEHGLVTARLDILINPGIVVPEHITRITKITNAMLRGKPKLDDVLDEILEFL
                     GDDILVSHNAEFDLGFLNANLRKRGRNPLNNPTIDTLALSRYLFPESRGHRLGVLARN
                     LEVFYDEDGAHRADYDAEVLNSIWQAMLTKLTEDDQYLTHAALAELKLTQAALQYLWP
                     AHATVYVKNKDGLRDLYKLVSVSHTEFSTDIPKTPRSMLLSHRDNLLIGSGCFNGEVF
                     ETAARFDEDRLLKVMEFYDFIEIQPYSNYSYLINMGELDSEEHLKKVIGDIKSAAQKL
                     GKPLVATGDVHYCLPSQKVFRDVYIVAKGLKNVN"
BASE COUNT         2693 a         1279 c         1688 g         2472 t
ORIGIN      
        1 aaactgaagc taaagaagaa ggtgaagctg ctccagaagt agcggaaaaa cctgctgctg
       61 cagctaaacc aaaaaaagaa aaagaagtta aagaaccgaa agctgaaaaa gctgaagagc
      121 caaaagtgaa agctgaacca aaagctaaag ctccaaaagc tccaaaagtt gaagaagtga
      181 aaactgaaga agtaaaaact gagaaagaag gagaataatt aatggcaagt ttaatcgata
      241 aaattaaaga attgcgtgaa cttaccggcg caggaatgat ggactgtaaa aaagccctcg
      301 ttgaaaacgg tgaagatgtt gataaagcta aagattggct tcgtgagcaa ggtatcacga
      361 ccgctgcgaa gaaaagcggc cggattgctg ctgaaggctt agcgcttgca aaaatagaag
      421 atggctacgc tgctattgtt gaagttaact gtgaaactga ctttgttagt cgcggtgatg
      481 actttaaagc gttagtgaac actgttcttg atttattgct caaacataaa cctgctaata
      541 aagaagcagc ggatgcaatt attaatccac ttttagttga agcaacagta aagattggtg
      601 aaaaattatc attccgtcgt tttgaactcg ttaaggttga agaccgtggt gcaacttaca
      661 ttcatatggg tggtaaaatt gctactgtcg tactcttaga taagaaaaat gacgaattag
      721 cgcatggctt aacaatgcac attgctgcga atagtccaag ctatattacc actgatgata
      781 ttaccgaaga agattacaat catgaacgcg gtattcaact tgaaattact aaaaacgatc
      841 cacgtttagc agacaaacca gaaaaaatgc ttaaaaacat cgttgaaggt aaaattagaa
      901 aatactttag tgatttagtc ttagccgaac aaacatattt aatggacgat acaaagaaag
      961 ttggtcaagc cctaaaagat cttgacaata aagtcttagt gatgttccgt taccaagtcg
     1021 gtgaaggaat tgaaaaacgt caagaagatt tcgccgctga agtctttggt caaattaaat
     1081 aataaaccac aaaaagaaaa caccttcggg tgtttttttg gaggaaaaaa gtatggtata
     1141 taaaagagtg ctcttaaaac taagtggaga agcccttgcc ggtgataaaa aaagcggaat
     1201 ttatgatcca aaagtattat ccgcgatttt aaattttgtc aaatcattcc gccatcatca
     1261 aaaatgggaa attggtatcg ttgttggcgc tggtaatatt tggcggggga aaaacgctga
     1321 acttattggt tttgaccctg ctgctgctga ttatatgggg atgactgcga catttttgaa
     1381 ctcactagcg ctgaaagatt attttacgca aaatgattta cctgctgttg tcttaaacgc
     1441 ttttgcggtt gaaggacttg cggaaaaaac tagtccagaa ttagcaaaaa aacatttagc
     1501 gaagggagaa gttgtcattt ttggtggtgg tacaggtaaa ccgttctaca cgactgatac
     1561 aggtgcagca ctccgcgcta ttgatatgaa tgctgaccta attattttcg taaaagacgg
     1621 tgttgacgct gtttacacta aaaatccgca tcaatataaa gatgcacaaa aaataaccca
     1681 agtcacttac caagaactaa ttgatcttaa agttgaagcg atggatttaa gcgccctaga
     1741 agtactaaat aaaacgaaca tcacaagttt tgtcattggt atggatgaag ttttaaactt
     1801 cgaaccactt gaagaacata tggaaattgg tacaattata acgaagaggt aaaaaatatg
     1861 gatgaatatg cattactcgc tgaagagcga atggaaaaat caattttgaa tttacaaagt
     1921 gaattttcaa cactccgcac cggacgggca agtgcggcgt tacttgaccg gattgaatgt
     1981 gattattacg gtgaaaaaat gccaattaat caaattagtt caattagtgt ccctgaaccg
     2041 cgtcaacttt taattaagcc ttatgataaa ggtgatatgc gttcaatttt agcggcaatt
     2101 aacgcctcaa cactggggat taacccgatt aatgatggcg atacaattcg cttaatctta
     2161 cccccactta ccgaagagcg gcgccgtgat ttagtgaaac aagctaaaaa gtttacggaa
     2221 gaagcaaaaa ttgccattcg taatattcgg cgtgacgcta atacgagtat taaaaatgat
     2281 aaagatttaa gtgaagacat gagcaaaaga gcagaagaag ctgtgcaaaa agttactgac
     2341 gaatatgtca aaaaagctga tgatttactt aaagaaaaag aagaagaaat cttaaccgtt
     2401 taatgtaaat taacaactat gaattaagcc ttttacaagg cttttttctt ttgtaacaca
     2461 ctagtgtgtt atcctaaaat taatataaga gccaaaagta ataagtaaat cgcggaaaac
     2521 attattattc tagcgaagaa gacctgcggc ttatgtgggt taagaagcga aaagtctata
     2581 ctgatacaat cataaaattt aatgaaaaag tagcgatctc tttcccatct tttgataaag
     2641 attatccgtt gtatccttat taaagctatt agtgaaataa attattttta caaacgcttt
     2701 tcttttttaa cattttaaaa atgttattta atgaaaatag ttattcttat tgtataattt
     2761 aacatatgag taagacgaag attacaaact atgaaaatta tggcattaaa catattgctt
     2821 ttattttaga tggtaatggt agatgggcaa aggcgcgtaa aagaccaaga acttatggtc
     2881 atgaaaaagg tttttccagt ctttttgatg ttggtattgc tgttagtaaa cgtaatatcc
     2941 cctttatgag tgtatacgct ttttctacag aaaactggtc acgaccaaaa aaagaagtcg
     3001 attttttatt tagaacactc gaaaaaagat tacatagtgt gttaaagaaa gtacaaaaac
     3061 ataatattaa aattgtgact tctggtgact taaaaagatt acctgaatca acacaacaag
     3121 cgattaatta cgcaaaagaa gaatcaaaaa ataatacggg caccacatta aatatttgta
     3181 taaattatgg tggtaaagat gaaatcgttc gtgcgattaa aagactagca gcagatgaaa
     3241 gtgttaatat tcaagcatta acacctgata cttttgaaaa ttatttagat acaagcggaa
     3301 tgccgccggt tgatctttta gtgcggacga gtggcgagat gcgccttagt aattatatgc
     3361 tgtggcaact agcctacgct gaatttatat ttaccccaac ttattggccg gattatgacg
     3421 aagagtgtct cgtccgtgat ttagaagaat acgcaaaaag aacgcgcagg tttggaggat
     3481 tatagtgatg gaaactaatg aaattaaacc aaaaaccaaa cggacttcaa cgattaaaat
     3541 atcactaaat gaaattagtg aagcaacaaa gaagtcaatg aagacccgca caattacttc
     3601 gattatttta gcggtaatcg ctgttcctgc cttgctttta ggtgggtggt actttttagc
     3661 gcttgtgttc tttgtgtcaa ttattgctgt gcttgaaatt atgcgtggca cgaaaatatc
     3721 aaaacgctac tggtgtgtgt atatcttagc gattgtttca actgttgccc ttatcttttg
     3781 gatttttatt aaaaataata taatggctaa tattaaggcc ggatactcgt ggtgggatat
     3841 ttcaaagtgg gtggtggaaa acggttttga atcaattcaa gtatcaagtt tcttacttgc
     3901 gaccatttta attggtctat ttatcttaat tgtgtttgat ccgcatcttg gttttgatat
     3961 cgcggcgtat ttattcttgt tagtgctctt tgccgagttt gggttccaag ctttcttatt
     4021 cttaaggctt tatccgcaat atattgctga tcgtcctgac attactttta acacgaatat
     4081 tattacgagc gcattcctta tcatttatgt aattttagga acaattatga atgatattgg
     4141 tgcttatttt atcggtgtgt tatttggtaa aaataagatg gtaccacgga tttccccaaa
     4201 taaaacatgg gaaggctttg ctggtggtgt tttcttttcg attgttgtta gttttttatt
     4261 cgccttaatt gtgtcgtgga ttggttatcc ggttttacca tttttgagtc ataaagagtg
     4321 gtattattta ttactcatct caattttaat gccgcttgcg gcaaatattg gtgacttatt
     4381 ctttagcgct accaaaagac atttagcgat taaggattat ggttcgattt tagttggtca
     4441 tggcggtatt ttagaccgtg ttgattcact tttaatggtt agtattgtga cggcaatgct
     4501 aatcatctta ataaataacg gatggagtat actcgtctag aaggaagtta gtatggaatt
     4561 tttaagtaca atattaaaca ttattttatt tttagtaatg cttggtgtat taatcaccat
     4621 tcacgaatta ggccacttta ttgctgcgaa gatatttaga gtgtatgtct ttgaatttgc
     4681 gattgggttt ggtcctaaaa ttttccgcaa gaaaaaagga gaaacttatt attcattacg
     4741 cgcattgccg cttgggggat atgttgcgat gctcggcgaa gaagatgctg tgcctgatga
     4801 atttgtaaat gaagaaattc ccaaagaacg gagtttaatc ggcattaatc gtggcaaaag
     4861 ggcattaatt atgtcagcgg gtattattat gaacttagtg ttaggctacg ctatattctt
     4921 attcgctaat ggggttttac cgcaaaccgg gttatcaatt cgtttagaag ttaaagaaga
     4981 tagtcctgta tatgcaatgg ggatacgcag tgatgaccgc attgatttta aagaaacgaa
     5041 gattgatggt tatacgattc agtacttcgg tgaagggaag cttgacgctg cagcacttga
     5101 accagaatat tatgttttat ttcaaccaaa gagtttagat gacttaactt ttggtggtga
     5161 gtcgcttgtc cttttaaaaa ttggcgcgac tgatttagcg agtgaaaatt cgttttacac
     5221 tgatttaaaa gatacagatg tgttaacctt taccgcaact ttcttacgcg gtgaaactta
     5281 tgaaactgcg gagcgtattg aaaaagtatt aacacttagt actgaaccaa aaaatcccga
     5341 taaattaaca gacgggtata aatttgtgtc cgctggtttt aatttaacga aatttaaata
     5401 tcgcaataac tttagtgaaa cattcaccgc cgcaaataaa gattttatcc gtagtgttac
     5461 ggcagtagcg cgcggcctca agtcattatt tagcgatggt cttaaaaatg ttagtggacc
     5521 agtggggatt tttaatattt ctagtcaaac acttaagaat catggtttag caccttattt
     5581 attcttatgg ggtttaatta gtattaactt agcgctcttt aacttattac cgtttccacc
     5641 acttgatgga tggcacttat tagtggtaac agttgaagcg attactcgtc aagaaattag
     5701 tcctaaattt aaacaaattg cctcaatgat tggtgcaatt cttttgattg gcttaacact
     5761 tgttatttta gcgaaagata tttttggctt cgtaggagtt attttataga tgggaaacgt
     5821 tagtgctcgt ttttttgcgt caattaatta tgaaaacgac aagaaaattg ggttaacgtt
     5881 tttagaatgg aaaaaagttg atgaagtgtg gcactacaca cttttaaaag aaaaaccgtg
     5941 gaattttgaa gactatctag attttagtga tgcgttaatg agtattcctt atgcatataa
     6001 agtgagttat gcttacacta aattaccgcg tattgaagat gctttagcgc tttttaaacc
     6061 gtggcattta catacttatc ttagtgaaag caactatgtt attatggtcg ttggcaatga
     6121 tacattacgc tttactttta ccgatgaagt tagtgcgctt gacgctaaag cggtgcttta
     6181 tgaattttct ttactcttaa aagaactatc ttataacttc gccttagaaa cagttgtcat
     6241 tagtagtgca caggaagaag acgaggaaga cgaattttca cttgggagtg aaagaccctt
     6301 tatcgaagat gatgatgacg atgaagaata ttttgatgaa gaagatgaca cgggtgtagc
     6361 agattgcgat cctctagatc caaatgaaaa ctgtgaaaga agtcttgaat atcacgacgg
     6421 gttagaagat aaactaaaag aaaattacga aaaaatggtt aaagagcggc aaaaaagaag
     6481 gcacgctaaa ggagctttta atcttaccga aattaaagat ttagaacata aagaagttga
     6541 tgaaggtgtg gactttgacg gtgaaatata tagttatgat aggcgtccga caaagcacgg
     6601 tgtcacttat acactcggta ttggcaaaga cgctcacggt atttcagtac gattttatat
     6661 taataaacgt ggcgcaaagc aagatgaagt gttaaaagca ctaaagacgg gacgctttgt
     6721 gcgtgtacaa ggttatatcg ctattgataa tttccgcggt caaactaaaa ttgttagagg
     6781 gcgcgacatt gatgaaattg ctgcaccacc attaccgaca gatgattacg aaggtcggaa
     6841 acgcatcgaa ttacatctac acactaaaat gtcagcgctt gatgccgtaa gtacgattga
     6901 tgaatattgt gaacttgcta aaaatatggg ccatgaagcg attgcactta ctgatcacgg
     6961 tgttgtgcaa gcctttcctg aagcccaaaa agccgggaag aaatatgata ttaaaataat
     7021 ttatggtagt gaactatatg ttatcgatga aacattaaaa gtcgttaata accctgcacc
     7081 aattatttta aataatgccc gatatgtaat ttttgactta gaaacaacag ggctaagtgc
     7141 tcgctataac gaaattattg aatttggcgc tgtgcgtgtg gagcacggcc tagtgacagc
     7201 gcggcttgat attttaatta atccaggtat tgtcgttcct gaacatatta cacgcattac
     7261 aaaaattaca aatgcgatgc tgcgcggtaa acctaaacta gatgatgtcc ttgatgagat
     7321 tttagagttt ttaggtgatg acattttagt gtcacataat gccgagtttg acttaggatt
     7381 tttaaacgct aatttgcgta aaagaggacg gaacccgctt aacaatccaa caattgacac
     7441 tttagcgtta tcgcgctatt tattcccaga atcacgtggt caccgattag gtgttttagc
     7501 ccgcaattta gaggtatttt atgatgaaga cggggcgcac cgtgcggatt acgatgcgga
     7561 agttttaaat agtatttggc aagcaatgct tacaaaatta acggaagacg atcaatattt
     7621 aacacatgcc gcccttgcgg agttaaaact aacacaagct gcgctccaat acttatggcc
     7681 agcgcacgct acggtgtatg tgaaaaataa agacggactt cgtgatttat ataaattagt
     7741 gagtgtgtct cataccgaat tctccaccga tattccaaaa acgccgcgct ccatgctttt
     7801 aagccatcgt gataatttac taatcgggtc aggatgtttt aacggtgaag tatttgaaac
     7861 tgcagcgcgc tttgatgaag accgcttgct taaagtaatg gaattttatg attttattga
     7921 aattcaacca tacagcaatt actcttactt aattaatatg ggtgaattag atagtgaaga
     7981 gcatcttaaa aaagttattg gcgatattaa aagcgctgcg caaaaattag ggaaaccgct
     8041 tgtggcaacg ggtgatgttc actattgctt accgagtcaa aaagttttcc gtgatgttta
     8101 cattgtcgca aaaggattaa agaatgttaa tc
//
LOCUS       DAOPCD010000020         8018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_8258_length_8018_cov_3.706266, whole genome shotgun sequence.
ACCESSION   DAOPCD010000020 DAOPCD010000000
VERSION     DAOPCD010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8258_length_8018_cov_3.706266"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PLQ55_01200"
     CDS             complement(<1..715)
                     /locus_tag="PLQ55_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPK28588.1"
                     /translation="MSFIPLNVFTNYALLQSALTLDDYLKTLKNRNIKAAGVSDPDTL
                     FSYPHFAKKSREFHIKPLYGVRLNFEGSSLVIYPQNEKGYRELLALLEVKNKRELTLQ
                     DLKENSTNLIVIIPSSAIHNLELKEQIMQKYFYNFAINTTSLYIGLERENAELTSFLR
                     EFSDKYNYALVAFPLIKYAKSEDHKTLLMIEAIREGKSFSIYDISSGPDYILSDAELN
                     NYYSENELKNTHLISDLINF"
     gene            complement(763..1752)
                     /locus_tag="PLQ55_01205"
     CDS             complement(763..1752)
                     /locus_tag="PLQ55_01205"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPK28589.1"
                     /translation="MHIYSKEIELKIKEYKKTTKAIFSLVEKYDKVAIFTHLSPDFDA
                     FGSQFGLGVYLRDHYPEKDIKIIGQSLPLYNGELYPEMDEVKDEWFNDDFLAIIVDTA
                     TLDRVSDNRYEKANHLIKIDHHPNVDPYGDICFVDVTSVAVAEMIAVMLLADGGTISK
                     DVASYLFSGIVGDSGRFKYASTTSLTFGIAEILTSLGIDLNYIYNRLYIQNLDDLRCI
                     GYILNNFKITKNGFAYYVMDEATLQELNITAERAKENVNVFADIRGIKVWAAITERTD
                     TNEWRVSIRSAGIPINEFAQKWRGGGHVQASGASLLNREEIDVFVKEMDEYIG"
     gene            complement(1762..2970)
                     /locus_tag="PLQ55_01210"
     CDS             complement(1762..2970)
                     /locus_tag="PLQ55_01210"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPK28590.1"
                     /translation="MSKLVLAINSGSSSLKFKLFKVPSEKVITSGLFERITQKMGAFR
                     IKTETGEVRRELPLPNHEVAVNVLTKALIEFKIVKSLDEINAVGHRVVQGGKYYNDSV
                     EFTDEVAAMVEKLSPFAPLHNPANLTGYEAFKKILPDALHVAVFDTSYHQTMPPEDYL
                     FPIPYEYYEKYDIRRYGAHGTSHKFIADEVTKINKRKPCNLIVCHLGNGASITAVKDG
                     KCVATSMGFTPLGGIMMGTRTGDLDPSVFYFVSKLEDKSPEEVYDMFNKKSGMLGVSG
                     ISSDARDVENALLQGNERAKHTFDLYSRRVADYIGQYHIRLGGTDTIVFTAGIGENVP
                     GIRKQIIDKVKDAMGIEFDDEQNSLTRGGIYGLLSTKNSKVKVYVIPTDEELMIVRDC
                     VRIAENKKKK"
     gene            3338..5395
                     /gene="glgB"
                     /locus_tag="PLQ55_01215"
     CDS             3338..5395
                     /gene="glgB"
                     /locus_tag="PLQ55_01215"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HPK28591.1"
                     /translation="MSFIDEFLMGFTTSAHKYFGAHFNPAKGKKKKSVTFRLYAPAAS
                     DVSVIGDFNGWDPNVHKMEKVDYRGIWEITIPELENYQNYKFRFRNAHGHYVDKADPY
                     AFFSEKAPGTASRLFDYEGFIWHDEDWMKSRCRGHDKPISIYEIHLGSWKGKENGRIL
                     SYEEIADYLIPYMRDNGFTHVEIMPITQYPFDGSWGYQATGFFSVDSRYGNPMQLMSF
                     VDRMHQAGFGVILDFVAVHFAIDHYGLIEFDGTRMYEYQDSEHTFSQWGSPQFDLGRD
                     PVRSFLISAIFYFLEYFHFDGIRIDAVSNIVYWHGNSDRGVNHGGVEFLKRLNGKVHY
                     HFPGVMMIAEDSTAFDGTTRDVEWGGLGFDYKWDMGWMNDTLRYYSKDPIYKKYDHNL
                     FTFSMHYFFSENFMLPLSHDEVVHGKGTIINKMFGNYDQKFALTRNLYTYQFAHPGKK
                     LTFMGNEIASFDEWNESKSLPWNLKSYPKHDSVSRLVRDLNLIYRSEKAMHVGEFDPR
                     NFRWLMVDNRDQSVFAFQRKYGEETLIFVFNMTGNYYEYYDIGVEEEGTYTEIFNSDK
                     DVYGGYNQYNGLPVTTHAGPGPEMRPYRLTLKLGSFAAMILKKDLPAKKEEPKVEEVK
                     AKKPAAKKAPAKKAATTKKAEPKATKTATKSTATKKATKSATAKKTTTKKATTAKTKK
                     DTK"
     gene            5432..7846
                     /locus_tag="PLQ55_01220"
     CDS             5432..7846
                     /locus_tag="PLQ55_01220"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPK28592.1"
                     /translation="MFTTTKSFIHEFKKRLTEKYGVGLDDSHITERYDILGSMVRDYA
                     NINWRKSRDAVNKKGKKQLIYFSMEFLIGRLLTNNLINLGIYNVVNKGLKQIGMNLHD
                     LEEQESDAGLGNGGLGRLAACFLDSIASLGYAGNGHCIRYEYGFFRQKIVDGKQVELP
                     DQWLVNGNVWEVRKPKHSVNVKFYGHAETYQKRNGEFGIRTVNPTIVKAVPYDMSVVG
                     YRNDVANTLRLWSAEPSDEQLPRDESFDDYLRTLREINRGLYPDDSTEKGKMLRLRQQ
                     YFLVSAGLQSAIRGHFRRHKTLRNFHEHYVFQLNDTHPILAIPELMRILLDDYEYGWD
                     ESWDIATKTFAYTNHTIMVEALERWPVRYVHHLVPRNYMIIEEIHRRSQREFVNKGIS
                     EGDRQNMAIIRDGMIHMTNLAIYASFSVNGVAALHTEILKAQTFKEFYSLYPEKFNNK
                     TNGITHRRWFVASNPELNKYLTSLIGDSYITNPDDFEKLLPFVKDKKVQRKFLQIKRL
                     KKQQLAAYIKRANGIKIDVDSIFDVQIKRLHAYKRQLMNVFRIMMLYFRMKSDADFRI
                     HPVTFIFGAKAAPSYYFAKKVIELINGVADVVNNDPEISPFMKVVFIENYGVKTAEII
                     IPATDISEQISTAGKEASGTGNMKFMMNGALTLGTLDGANVEIHERVGDEHSFIFGLT
                     EPEVKALRESGTYNPWDVYHNDHEIRRILDSLIDGTWSQGNPHTFKAIFDEVMNKGDE
                     YLILKDLPAYFKRTDEIVTYYRKKSSWAESALYNIAKSGFFSSDRTIHQYIDEIWHID
                     KVKNGN"
     gene            7836..>8018
                     /locus_tag="PLQ55_01225"
     CDS             7836..>8018
                     /locus_tag="PLQ55_01225"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019391239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPK28593.1"
                     /translation="MEINLAPLLLKQRELDADINSRHNVTHESTKTERFLALLVEIGE
                     LANATRAFKYWSLKKSE"
BASE COUNT         2590 a         1437 c         1515 g         2476 t
ORIGIN      
        1 caaagttaat taaatcacta attagatgcg tatttttaag ttcattttca ctataataat
       61 tattaagttc agcgtcactt aaaatataat cgggaccact agaaatatca taaatggaaa
      121 acgatttacc ttctctaatt gcctcaatca ttaaaagtgt tttatggtct tcacttttag
      181 catatttaat aagcgggaac gctactagtg catagttata tttatcacta aattctcgca
      241 aaaaggatgt taattctgca ttttcccgtt ctaaaccgat gtataatgat gttgtattaa
      301 tggcaaaatt ataaaaatat ttttgcataa tttgctcttt taactctaaa ttatgaatag
      361 cggaagaggg aataataaca attaaatttg tgctattttc ttttaaatct tgcagcgtca
      421 attcgcgttt atttttgact tcaaggagcg ctaaaagttc acgatatccc ttttcgtttt
      481 gaggataaat aactagtgaa gaaccttcaa aattaagcct aacaccatag agtggtttta
      541 tatgaaattc cctacttttt ttggcaaagt gcggataact aaaaagggta tcaggatcac
      601 taacacccgc tgctttgatg tttcgatttt taagtgtctt taaataatcg tccaaagtaa
      661 gtgcactttg aaggagcgca tagtttgtaa atacatttaa agggataaaa gacatataat
      721 tattttaaca tattgaagag acaagaaatg aaaataggct tattagccta tatattcatc
      781 catttctttc acaaatacat caatttcttc acggtttaaa agtgaagcac cactagcttg
      841 cacatggcca ccgcctcgcc atttttgggc gaattcatta atcggtatac cagcactgcg
      901 tattgatacc cgccactcgt ttgtgtcagt gcgttcggta atggcggccc agactttaat
      961 cccgcgaata tcagcgaaaa cattaacatt ttcttttgcg cgttcagcag taatattgag
     1021 ttcttgcagt gtagcctcat ccatcacgta ataggcaaaa ccatttttcg taattttaaa
     1081 attatttaag atataaccaa tacagcgtaa gtcatctaag ttttggatgt ataaacgatt
     1141 gtatatataa tttaaatcaa tgcctaaact tgttaagatt tcggcaatac cgaaggtaag
     1201 tgaagtagtg gacgcatatt taaagcgtcc agaatcacca acgataccac taaatagata
     1261 actagcgaca tcttttgata tagtgccgcc atctgctaaa agcataacgg caatcatttc
     1321 ggcgacggca acactagtaa catcaacaaa acatatatca ccgtagggat caacatttgg
     1381 atgatggtca attttaatga gatggttagc tttttcataa cgattatcac taacacggtc
     1441 aagcgtcgcg gtatcaacaa taattgctaa aaaatcatca ttaaaccatt catcctttac
     1501 ttcatccatt tcgggataaa gttcaccgtt ataaagtggt aaactttgcc caataatttt
     1561 gatgtctttc tcgggataat ggtcgcgtaa atatactcct aaaccgaatt gagaaccaaa
     1621 ggcgtcaaag tctggtgaaa ggtgcgtgaa aatagctacc ttatcatact tttcaacaag
     1681 actaaaaatt gctttggttg tttttttata ctctttgatt ttaagttcaa tttcttttga
     1741 atatatgtgc attaattaca cctatttttt ctttttattt tcagcgattc gcacacaatc
     1801 tcgaacaatc attaattctt catcagtcgg aatcacatat acttttactt tactattttt
     1861 cgtggaaagt aaaccataaa ttccacctct agttaagcta ttttgttcgt catcaaattc
     1921 aatgcccatc gcatctttaa ctttgtcgat aatttgttta cgaatgcctg ggacattttc
     1981 accgatacca gccgtaaaga caatggtgtc agtaccacct aaacgaatat gatattgacc
     2041 aatataatca gcgacacgcc gactatataa atcaaaggtg tgtttagcgc gttcattgcc
     2101 ttgaagaaga gcattttcaa catcgcgtgc atcactagaa ataccgctga caccaagcat
     2161 tcctgacttt ttattaaaca tgtcataaac ttcttcgggt gatttatctt ctaatttact
     2221 aacaaaataa aagacgcttg gatcaaggtc accagttcgt gtacccatca tgatgcctcc
     2281 taaaggagta aagcccattg atgtagcaac acattttccg tctttaacag cagtaatact
     2341 tgcgccgttc cctaagtgac agacgataag attacacggt ttccttttat taatttttgt
     2401 aacttcatca gcaataaatt tatgtgaagt accgtgtgct ccgtagcgac ggatgtcata
     2461 cttttcataa tattcataag gaatggggaa taaataatct tctggtggca ttgtttgatg
     2521 gtaagaagta tcaaagacag caacatgcag tgcatctggc aagattttct taaaagcttc
     2581 gtagcccgtt aagtttgcgg ggttatgaag tggggcaaaa ggactaagtt tctccaccat
     2641 tgccgcgact tcatcggtaa attcaacgct atcattgtaa tatttaccgc cttgaacaac
     2701 acggtgaccc actgcgttaa tttcatctaa tgatttaacg attttaaatt caattaaagc
     2761 cttcgttaaa acattaaccg ctacttcgtg atttggtagt ggtagttcgc gtcttacttc
     2821 acccgtttcg gttttaatgc gaaacgctcc cattttttgt gtaattcttt caaaaagtcc
     2881 acttgtaatt actttttcac ttggaacttt gaaaagtttg aattttaagg aactgcttcc
     2941 cgagttaata gctaacacta attttgacat atatttcacc tctcatttta ttattataca
     3001 caaagacaag ttagcgtgct gtgattttgt tacaaaaaag ttgattaatt atttccccta
     3061 tattattaat aataagagca aattatcttt ttatcttgtt tgcggattaa tattttgcga
     3121 taaataattt ggcaataagc tagtaacaac tctaaaattg taataactca aatatttaaa
     3181 aaataccgat aatgcataaa atttcaataa attgaatatt attcgtctaa atttgtcggt
     3241 aaattttcat tttctgtcaa aattgcgata attccggtgt ttaaactacc attcttcgtc
     3301 cttttttatt ataataataa aacgagggag gttgattatg agttttatag atgaattcct
     3361 gatggggttt acaacgagtg cacataaata ttttggcgct cattttaatc ccgcgaaagg
     3421 taaaaagaaa aaaagtgtaa catttcgtct ctatgcccca gcggcaagtg atgtaagtgt
     3481 gattggggat tttaacggtt gggatccaaa tgtccacaag atggagaagg tcgattaccg
     3541 tggtatttgg gaaattacta ttcctgaatt agaaaattac caaaactata aattccgatt
     3601 ccggaatgcc catggtcatt atgtagataa agctgaccca tatgctttct ttagtgaaaa
     3661 agcaccaggt actgcttcac ggctatttga ttatgaaggt tttatttggc acgacgaaga
     3721 ttggatgaaa agtagatgta gaggtcatga taaaccaatt agtatttacg agatccattt
     3781 aggcagctgg aaaggaaaag aaaatgggcg gattttatct tacgaagaaa tcgctgatta
     3841 cttaattcct tatatgcgtg ataatggctt tactcatgtc gaaatcatgc cgattacaca
     3901 atatccattt gacgggtcat ggggttatca ggctactggt ttctttagtg ttgatagtcg
     3961 ttatggtaac ccaatgcaac ttatgtcatt tgtcgaccgt atgcaccaag ccggttttgg
     4021 cgttatttta gactttgttg ctgttcactt tgccattgac cattatggtt taattgaatt
     4081 tgatgggaca agaatgtatg aatatcaaga cagtgagcat acatttagtc aatggggttc
     4141 cccgcaattt gatttagggc gcgatccagt tcgtagcttc ttaattagtg cgatttttta
     4201 tttcttagaa tacttccatt tcgacggaat tagaattgac gctgtcagta acattgtcta
     4261 ctggcacggt aatagtgacc gcggagtaaa tcacggcggt gttgaattct taaaacgact
     4321 caacggtaaa gttcattacc atttccctgg tgttatgatg attgctgaag actcaacagc
     4381 gtttgacggt acaacgcggg acgttgagtg gggtggttta ggctttgact ataaatggga
     4441 tatggggtgg atgaatgaca ccttacgcta ttatagtaaa gacccaattt ataagaaata
     4501 tgaccataac ctctttacat tctcgatgca ttatttcttt agtgaaaact ttatgttacc
     4561 actaagtcac gacgaagtag tgcacggtaa agggacaatt attaataaga tgtttggtaa
     4621 ctatgaccaa aagtttgcct taacgcgtaa cttatatact tatcaattcg cgcatcctgg
     4681 taaaaaactt acctttatgg gtaacgaaat tgctagcttc gatgagtgga atgaaagtaa
     4741 gagtttaccg tggaacttaa agtcatatcc aaaacacgat tcagttagtc gcttagtgcg
     4801 cgacttaaac ttaatttatc gtagtgaaaa agcgatgcat gttggtgaat ttgaccctcg
     4861 taactttaga tggttaatgg tcgataatcg tgatcaaagt gtgtttgcgt tccaacgtaa
     4921 atacggtgaa gaaacattaa tctttgtctt taatatgact ggaaattatt acgaatatta
     4981 cgatatcggt gttgaagaag aaggtactta cactgaaatc tttaatagtg ataaagatgt
     5041 ctatggtggt tataaccaat ataacggttt accagtgaca acacacgctg gtccaggacc
     5101 agaaatgcgt ccataccgct taacacttaa acttggttca tttgccgcga tgattttaaa
     5161 gaaagattta cccgcaaaga aagaagaacc aaaggtcgaa gaagttaaag caaagaaacc
     5221 agcagcgaaa aaagcgcctg ctaaaaaagc agcgacaaca aaaaaggctg aacctaaggc
     5281 aacaaaaact gccacaaaga gcaccgctac taaaaaagca acaaaaagtg ctacagccaa
     5341 aaagactaca acgaaaaaag caactactgc taaaacaaaa aaagacacta aataatttag
     5401 tgagttaaat aaataaagga aggaataatt tatgtttaca acaacaaaaa gttttattca
     5461 tgaatttaaa aagcgattaa cagagaaata cggcgttggc cttgatgata gtcacatcac
     5521 tgaacgttat gacattttag gttcaatggt aagagactac gcgaacatca actggcgcaa
     5581 aagccgtgac gctgttaata agaaaggtaa gaaacaatta atttatttct caatggaatt
     5641 tttaattggt cgcttactga caaacaacct aatcaactta ggaatttata atgtcgttaa
     5701 caaaggccta aaacaaattg ggatgaatct tcatgattta gaagaacaag aaagtgatgc
     5761 tggccttggt aatggtggtt taggtcgcct tgccgcatgt ttccttgatt caattgcgag
     5821 tcttggttac gctggaaacg gtcactgtat tcgttatgaa tacgggttct tccggcaaaa
     5881 gattgtcgac gggaaacaag tcgaattacc cgatcaatgg ttagtcaatg gcaacgtctg
     5941 ggaagtacgg aaaccaaagc actcggtgaa tgttaagttt tacggtcacg ctgaaactta
     6001 tcaaaaacgc aatggtgaat ttgggattag aactgttaat cccactatcg ttaaagcagt
     6061 gccctatgac atgagtgttg tcggctatcg taatgacgtc gcaaatactc tccgcttatg
     6121 gagcgctgaa ccaagtgatg aacaattacc acgtgacgaa tcatttgatg attatttacg
     6181 gacactccgc gaaattaatc gcgggttata tcctgatgat tctaccgaaa aaggtaaaat
     6241 gctccgctta agacagcaat atttcttagt cagtgctggc ttgcaaagtg cgattcgcgg
     6301 tcatttccgt cgtcataaaa cgttacgtaa tttccatgaa cactatgtgt tccaacttaa
     6361 tgatacgcac cccattttag caattccgga attaatgcgt attttacttg atgattatga
     6421 atatggctgg gatgagtcgt gggatatcgc tactaagact tttgcctata caaaccatac
     6481 aatcatggtc gaagcacttg aacgctggcc agtgcgttat gtccatcatt tagtgccacg
     6541 caactacatg attattgaag aaattcatcg tcgtagtcaa cgcgaattcg ttaataaagg
     6601 catcagtgaa ggtgatcgcc aaaatatggc aattatccgc gacgggatga ttcatatgac
     6661 aaacttagcg atttacgcta gttttagtgt taacggtgtc gcagcgctac acactgaaat
     6721 cttaaaagcg caaacattta aagaattcta cagtctttat cctgagaaat ttaataataa
     6781 aacaaacggg attacgcatc gtcgctggtt tgtggcaagt aacccggaac ttaataaata
     6841 tttaacaagt cttattgggg actcttatat tactaatccc gatgactttg aaaaactctt
     6901 accttttgta aaagataaga aagtgcaacg gaaattctta caaattaaac gcctcaaaaa
     6961 gcaacaactt gccgcctata ttaagcgcgc taacggcatt aaaattgatg ttgattcgat
     7021 ttttgatgtg caaattaaac gtttacacgc ttataaacgg caattaatga atgtatttcg
     7081 cattatgatg ctttatttcc ggatgaagag tgatgctgat tttagaattc atcctgtcac
     7141 ctttatcttt ggcgcgaaag ccgcaccaag ttactacttt gcgaagaaag taattgaatt
     7201 aattaacggt gtcgctgatg tcgttaacaa tgatccagaa atttcgccat ttatgaaagt
     7261 tgtctttatt gaaaactatg gcgtcaagac tgccgaaatt attattcctg cgaccgatat
     7321 tagtgaacaa attagtaccg ctgggaaaga agcaagcggc actggtaata tgaagtttat
     7381 gatgaatggt gccttaactt tagggacact agacggcgcg aatgtggaaa ttcatgagcg
     7441 cgtaggggac gaacattcct ttatctttgg cttaactgag ccagaagtga aagcacttag
     7501 agaatcaggt acttataatc cgtgggatgt ttaccataat gaccatgaaa ttcgtcggat
     7561 attagattca ttaatcgacg gtacatggag tcaaggtaac ccccatactt tcaaagcaat
     7621 atttgacgaa gtaatgaata agggtgacga gtacttaatc ttaaaagatt taccagccta
     7681 ctttaaacgc acagacgaaa ttgtcacgta ttatcggaag aaatcaagct gggcggaaag
     7741 tgcgctctat aatattgcta agagcgggtt ctttagcagt gaccgcacaa ttcatcaata
     7801 tatcgatgaa atttggcata ttgacaaggt aaaaaatgga aattaatctt gcaccattac
     7861 tccttaaaca acgtgaactt gacgctgata taaatagtcg tcataatgtc actcacgaaa
     7921 gtactaaaac ggagcgcttt ttagcgctcc ttgtggaaat aggtgaactc gctaatgcaa
     7981 cgcgtgcatt taaatattgg tcgcttaaaa aaagtgaa
//
LOCUS       DAOPCD010000021         7762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_8539_length_7762_cov_5.110938, whole genome shotgun sequence.
ACCESSION   DAOPCD010000021 DAOPCD010000000
VERSION     DAOPCD010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8539_length_7762_cov_5.110938"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLQ55_01230"
     CDS             complement(<1..255)
                     /locus_tag="PLQ55_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28594.1"
                     /translation="MKQKKARRHSGKKYLWYSILCVMLLVLAFSTYVLMSFGLIPMTG
                     NKTLDIIIGILLVIISIASIVCGIIAFVYYKRNSNQIRFNY"
     gene            complement(236..1045)
                     /locus_tag="PLQ55_01235"
     CDS             complement(236..1045)
                     /locus_tag="PLQ55_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28595.1"
                     /translation="MPKHKQNSEAAPKKKGRIGKITSVVLGAIVVLLIVLQVVGQSTA
                     KNNHGVMRYGNYQSLRVLTDSMEPTYKVDTMVFVKRVDPKTLKGPSSEGALDGDVITF
                     VRNYHGSYDLPGDKRIVTHRIHRIEVQADGRVYFRTLGDNIHAKECPESGCTLEARGD
                     WVRDTDILGKVVGQSMVLGKFSVLLANPIFMLVFAMVPLLIVFGSSLMDLLKQLKSTE
                     GSEATPSGISEQQAEFERIKEQEKLRLLIELEKEKLRKELEGGIKDETKES"
     gene            complement(1023..2921)
                     /locus_tag="PLQ55_01240"
     CDS             complement(1023..2921)
                     /locus_tag="PLQ55_01240"
                     /inference="COORDINATES: protein motif:HMM:NF021352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2357 domain-containing protein"
                     /protein_id="HPK28596.1"
                     /translation="MADENKVTIKQEQNVVAEEVAKEETAPKTKKDKPLKVAGAQEKV
                     KKAPIKDKAKKAADTKPKQTKKVASKKSDTKVEKEKVDAKPKAEKAKKAKADPKKADA
                     KPETEKVAEKALDTEAPAAKEKEPKEAPKKEAPKKAVPRRRRLSAMELRKREALKGYK
                     RFTKVRSRYYSFLNRTVKQSKPTTNLYNTIANKAPTLSGITRNEVKRFDPEFIEKIER
                     VIPSLEEIVVNPHKFINEFAEVVQVEKARKLTPRAVKYMAQNADHVSEVLDDGRVIPK
                     KILNVYVDDDLKIYENRFIMTLINRLQIFIELRHKYIREHGDTRNSDQITIKREVQVE
                     DSLFTVDATITMSVPSDDEGARESNMDLLERLENIRRRTQFLVNSRFMREMAKAVPVS
                     EPIAQTNIIRLNWAYQDAFKLWNFIHRYEELGISYTTTQAKIEFDKKYLDKINQFSLN
                     SFFTIETEHARLALSNVEQKNIRPIIEAGDLNYELSDERFAKPGLPMRVDARVETAEQ
                     EAARLKREATREKARLKREEAKRKQKEREALKKARAKERALKRKLEAAERAKRRKEEA
                     KQRQIEKNMERARLRAEKERLLAERKAYQKLMMTEAERLRRARKAVAAQATEERKKAI
                     EDAKAQAK"
     gene            complement(2929..4449)
                     /locus_tag="PLQ55_01245"
     CDS             complement(2929..4449)
                     /locus_tag="PLQ55_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28597.1"
                     /translation="MNAFFSYIRDTFESFWANFVEWLAAVFVEPWKLVSDDFGTYRTI
                     IEMYMDSFGFWGWFFFVILMILNVAVIGGLIFLLVLLIRRLVKYSSKNYDKAKLIAEV
                     QRLNKELFFAIQEKNEVLKLKAESLGLEDPSLVEGDKTSVVIDTFPKLGAVDLKYKDK
                     DTDFITPVLDREITLEQIAVRYRYFAASQLGLFYDIDLVRVLFAAMGTVKIIILEGIS
                     GTGKTSLPYSLGKFFLNDVAICSVQPSWRDRTELIGYYNEFTKKFNESEFLRAIYEAS
                     YRKDVNIVILDEMNLARIEYYFAEFLSIMEMPNPQEWYVEVTKTADEMNPVQLVNGKL
                     LIGQNIWFFGTANNDDSTFTITDKVYDRAISITLNERGQMFDAEFTEPLKIPYEHLRR
                     LFDEAQTNFPLSKANANKFSKLDEYVVNKFRIGFGNRILRQIHTFVPIYVASGGTEPE
                     ALDFMFSSKILKKFRSLNLAFMRDELSALRAFIERTFGKNTFKHSLNQIDDFMTRA"
     gene            complement(4470..5693)
                     /locus_tag="PLQ55_01250"
     CDS             complement(4470..5693)
                     /locus_tag="PLQ55_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28598.1"
                     /translation="MAEEINLSEHPLLEVANDIIREEKRRKRTKKVVAVSAFIIIVFI
                     IISYLGYAIGYYIANATSLSLLSKDPEARFAQVGFRLSEPLTVTEQHKYNLQSDEGWV
                     GGEGYVYWTTQNVSPQTMSFVLEHEGYSNTNRLSPVTSGSFTKDEDPYLPLTLYEAPK
                     QNRAERHNLNLNVADPKDYFGFTVLFRLLIDEDRAGTIPLDNIAIYFNRDTKVHGQNE
                     LPKALRFGFQSGAKKDIISPGRTLLEENDYTGSIAVGGRLDLDDPRAPASLTGFYDYT
                     FGLDGAKYGESGEQIYEIAYGEFEQPLTNENWGEPLAEAEEKLEHGDVFHAVTYKGVR
                     PLINVVPQRQYYTSFQKYMLDGDKGEPLCYTNNLGVAEMNIKFWLEGWDLATTNKVVN
                     KSFGMQLHFVAKRAE"
     regulatory      complement(5881..5960)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(6059..6451)
                     /locus_tag="PLQ55_01255"
     CDS             complement(6059..6451)
                     /locus_tag="PLQ55_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28599.1"
                     /translation="MTGQEKKKKFKLPSWATYLLLGLELALMVALVTISIVALKNVQA
                     GSGGPFIKWLIGNRVWFFVLIVLPLIVLFLFNIYLLIKVMNEASSKEFKQMTKDELLE
                     EARKQALEELRKELAKQNEPAPETKPEE"
     gene            complement(6643..7509)
                     /locus_tag="PLQ55_01260"
     CDS             complement(6643..7509)
                     /locus_tag="PLQ55_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28600.1"
                     /translation="MNKRNKRLGTVLTLLFISAIAATSGTLAWFTTVRTAGITYGDAE
                     VYMRDSDLVVTYKSSLNSLSATSELDGITKITLTGTNKVTDISGNGINFYKPRWNSDN
                     VTAAVINTVPSTNPGDADGYFIDFTLTLSRSPGEDPLNLYVFLGQNTAILPADPANPK
                     DVGALKALRMAVISYDNNDSATGTPSVDILYAPEAEINPEYLQLGSGGAYGLTTHELV
                     PATVKSDSFETHYTIADSQAVYPEIAVLPAGTGTADVTFRVWIEGTDIHAAESIIGGK
                     FKIKLDIYSTGV"
BASE COUNT         2212 a         1610 c         1298 g         2642 t
ORIGIN      
        1 ataattaaag cgaatttggt ttgagtttcg cttataatat acaaaagcaa taatcccaca
       61 tacaatacta gcgatactta taattactaa taaaatgccg attataatat caagtgtttt
      121 gttccctgtc ataggaataa gaccgaagga cattaaaaca taagtactaa aagcaagcac
      181 taaaagcatt acacataaga tgctatacca taaatacttt tttccgctat gtcttctagc
      241 tttcttttgt ttcatctttt atgcccccct ctaattcttt tcgtaatttt tctttttcta
      301 attcaattaa taatcgtaat ttctcttgtt ctttaattct ttcaaattcg gcctgttgtt
      361 cacttatgcc actcggcgtg gcttcactcc cctcggtact ttttaattgt tttaggaggt
      421 ccataagtga agaaccaaac acaattaaga gtggtaccat cgcaaacaca agcataaata
      481 ttggattagc gagcaacaca ctaaatttac caagtaccat actttgacca acaactttgc
      541 ctaaaatatc cgtgtcacgc acccagtcac cacgcgcttc taatgtacaa ccactttctg
      601 gacactcttt agcgtgaata ttatcaccaa gcgtccggaa gtaaacgcgg ccatctgctt
      661 gcacctcaat acgatgaatc cggtgagtga caatgcgttt atcaccaggt aaatcataac
      721 ttccgtgata attacgcaca aaagtaatga cgtccccatc taatgctcct tcgcttgatg
      781 gtccttttag tgtttttgga tcaacacgtt ttacaaatac cattgtatca actttgtatg
      841 ttggttccat tgaatcagtt aaaacgcgta atgactgata attaccgtaa cgcatcactc
      901 catgattatt tttagcggtt gattgaccaa cgacttgtaa aacaataagt aaaacaacga
      961 tcgcccctaa aacaacgctt gtaattttac caatacgccc ttttttcttt ggcgctgctt
     1021 cactattttg cttgtgcttt ggcatcttca atcgccttct ttctttcttc agttgcttgt
     1081 gcggcaacag ccttacgtgc tcggcgtaag cgttctgctt ccgtcatcat taatttttga
     1141 tatgctttcc gttcggcaag taagcgttct ttctccgcgc gtaagcgtgc ccgttccata
     1201 ttcttttcaa tttgccgttg ttttgcttct tctttgcgcc gtttagcgcg ttcagcagct
     1261 tctaacttgc gtttaagcgc tctttctttt gcccgcgctt tctttaaggc ttcacgttct
     1321 ttttgtttac gttttgcttc ttcgcgttta agtctagctt tttcgcgagt agcttcacgt
     1381 tttagtcgcg ctgcttcttg ttcagcagtt tcaacacgag cgtcaacacg cattggtaat
     1441 cctggtttag caaaacgttc atcacttaat tcataattta agtcgcccgc ttcaataatc
     1501 ggacgaatat tcttttgctc aacattactt aaagcaaggc gcgcatgctc tgtttcaatt
     1561 gtaaagaatg aatttaatga aaattgatta atcttatcta aatatttttt atcaaattca
     1621 attttggctt gggttgttgt atatgaaata cctagttctt catagcgatg aataaagttc
     1681 cataatttaa aagcatcttg atacgcccag tttaaacgaa taatatttgt ttgtgcaatt
     1741 ggctcagaca caggtacggc tttagccatt tcacgcataa agcggctatt aactaagaat
     1801 tgtgtccgtc ttctaatatt ttctaaacgt tcaagtaaat ccatattact ttcgcgtgcc
     1861 ccttcgtcat cactaggcac tgacattgta attgttgcat caactgtgaa gagcgaatct
     1921 tcaacttgta cttcacgctt aatcgtaatt tggtcactat tacgcgtatc accatgttct
     1981 ctaatatatt tatgccgcaa ttcaataaag atttgtaacc gattaattag tgtcataatg
     2041 aaacggttct cataaatctt taagtcgtca tcaacataaa cgttaagaat ctttttgggg
     2101 ataacacgac catcatctaa tacctcagat acatgatcag cattttgcgc catatacttg
     2161 actgctcgcg gggtaagttt ccgcgctttt tctacttgca cgacttcggc aaattcatta
     2221 ataaatttat gcggattaac gacaatttct tcaagagaag gaatcacgcg ttcaattttt
     2281 tcaataaact ctgggtcaaa acgttttact tcattacgcg taataccaga caaagttggt
     2341 gctttattag caatcgtatt gtataaattc gtcgttggct tactttgctt aacagtccga
     2401 tttaaaaatg aataataacg gctacgaacc ttagtaaaac gcttgtagcc ttttaatgct
     2461 tcacgctttc ttaattccat cgccgaaagt cgtcgacggc gtggcacagc tttttttggt
     2521 gcttcttttt tgggcgcttc tttcggttct ttttctttag cggcaggtgc ttcagtatca
     2581 agtgcttttt ccgctacctt ctcagtttca ggcttagcgt cagctttttt agggtcagcc
     2641 tttgcttttt tcgccttttc agctttaggt ttagcgtcca ctttttcctt ttcaacttta
     2701 gtgtcacttt ttttactagc aactttttta gtttgttttg gtttggtgtc cgctgctttt
     2761 tttgctttat ctttaattgg cgcttttttt actttttctt gggctccagc cacttttaaa
     2821 ggtttgtcct tttttgtttt aggcgcagtt tcttccttag cgacttcttc cgctacaaca
     2881 ttttgttctt gtttaattgt aactttattc tcgtctgcca ttatttacct acgctctcgt
     2941 cataaaatca tcgatttgat taagtgaatg tttaaatgtg tttttaccaa atgtccgttc
     3001 aataaaggcg cgtaacgcac ttaattcgtc acgcataaac gctaagttaa gtgatctaaa
     3061 tttctttaaa attttggatg aaaacataaa gtcaagtgct tctggttcag tcccgccgct
     3121 agcgacataa attggtacaa atgtatgaat ttgccgtaaa atccggttcc caaatccaat
     3181 gcggaattta ttcacaacat attcgtcaag cttactaaac ttattagcgt ttgctttgga
     3241 aagtgggaaa ttagtttgtg cttcatcaaa taaccgccgt aagtgttcat acggaatctt
     3301 taatggttca gtaaattcag catcaaacat ttgtccacgt tcgtttaatg taatactaat
     3361 cgcacggtca tagactttat cggtaatcgt aaatgttgag tcgtcattat ttgcggtacc
     3421 aaagaaccaa atattttgcc caattaataa cttaccattc actaattgca ctgggttcat
     3481 ttcatctgct gtctttgtta cttcaacata ccactcttgt gggttaggca tttccataat
     3541 cgataagaat tccgcaaagt agtattcaat tctcgctaag ttcatttcgt caagaataac
     3601 aatgtttaca tctttacgat aacttgcttc gtaaatcgcc cgcaagaatt cactttcgtt
     3661 aaacttcttc gtaaattcgt tatagtaacc aatgagttcc gtacggtcac gccaggacgg
     3721 ttgcacacta caaatcgcga cgtcatttaa gaagaattta cctaaggagt aaggtaaaga
     3781 agttttacca gtaccagaga taccttctaa aataataatt ttaaccgtac ccattgcggc
     3841 aaagagcaca cgcactaagt cgatatcata aaataaccca agttgcgatg cggcaaaata
     3901 acgatagcgc actgcaattt gttctaaagt aatttcgcgg tctaataccg gtgtaataaa
     3961 atcagtgtct ttatctttat attttaagtc cactgcccca agttttggga atgtatcaat
     4021 aacgaccgaa gttttgtcac cttccacaag tgaaggatct tcaagaccta atgattcagc
     4081 tttcagttta agtacttcat ttttctcttg aatggcaaag aagagttctt tgttaagccg
     4141 ttgtacttcg gcaataagtt tcgctttatc ataatttttg cttgaatact taacaagtcg
     4201 acgaataagg agcactaata aaaagattaa gccaccaata acggccacat taagaatcat
     4261 taagattaca aagaaaaacc aaccccaaaa tccaaatgag tccatgtaca tttcaatgat
     4321 tgtacgataa gtaccaaagt catcggacac tagtttccat ggttcaacaa atacagcggc
     4381 taaccattcg acaaagttag cccagaatga ttcaaatgtg tctcggatat aactaaaaaa
     4441 tgcgttcata atatcaccat ttcctcttct tattcagccc ttttagcgac aaagtgtaat
     4501 tgcattccaa aagacttgtt tacaacttta tttgtggtcg ctaagtccca gccttctaac
     4561 caaaatttaa tattcatttc tgccacacca aggttatttg tgtagcaaag cggttcacct
     4621 ttatcaccat caagcatata tttttggaaa cttgtgtaat attggcgctg tggtacaacg
     4681 ttaataagcg gacggactcc cttgtaagtc accgcatgga aaacatcacc atgttctaac
     4741 ttttcttctg cttccgcaag tggttcaccc cagttttcat ttgtgagtgg ctgttcaaat
     4801 tcgccataag ctatttcata aatttgttca ccactttcgc catacttcgc cccatcaaga
     4861 ccaaatgtat aatcataaaa tcctgtaagt gacgctggag cacgcgggtc atctaagtca
     4921 agtctgccac caacagcaat tgaacctgta taatcatttt cttctaataa agtgcgccct
     4981 ggactaatga tgtctttttt agctccactt tggaaaccaa aacgcaatgc ttttggtaat
     5041 tcattttgac catggacctt cgtgtctcta ttgaagtaaa tggctatatt atcaagcggg
     5101 attgttccgg ctcgatcttc gtcaattaaa agtctaaata atactgtaaa cccaaaataa
     5161 tcttttggat ctgctacatt taagttcaaa ttgtggcgtt ctgcacggtt ttgcttcggt
     5221 gcttcataaa gcgtaagtgg taaatatggg tcttcatctt tcgtaaaaga accagaagta
     5281 actgggctaa gtctatttgt atttgaatag ccctcatgct caagcacaaa actcatcgtc
     5341 tgtggcgaca cgttttgcgt tgtccaatac acataacctt caccaccaac ccaaccttca
     5401 tcagactgta aattgtattt atgttgttct gtaactgtta gcggttcact aagtctaaac
     5461 ccaacttgcg caaagcgagc ttctgggtct tttgacaata aggaaagcga ggttgcgttt
     5521 gcaatatagt aaccaattgc ataccctaag tatgaaataa tgataaaaac aataatgatg
     5581 aacgctgaaa cagcgacaac ttttttagtg cgtttccgcc gcttttcttc acgaataatg
     5641 tcgttagcaa cttcaagaag tggatgttca cttaaattaa tttcttctgc cacaatattc
     5701 tccgttttct tataccgcta aaacaatcaa tgatgtttta gtgatatatg aacattcttt
     5761 attattttaa cacgaatgtc cgtgcacact atcctaattt tataatttga tagtggcgat
     5821 taaataaaaa tgatgctaac cactctttgc cagaaataat aacattcatc tttatttctt
     5881 cgaggcaact ggctaccatc acacttttag gccctatagt tttgcgtcct tagatttctc
     5941 taagtttgcc ttttctgtag ttattatatg ttgtacatat aaaagtgtca agaactatct
     6001 aatttaagta ttaaaaaagc cgtattttct acggcaattt taagtatttt gtgattaatt
     6061 attcttcagg tttcgtttca ggagcgggtt cgttttgttt tgctaactct ttacggagtt
     6121 cttcaagcgc ttgtttgcgc gcttcttcaa gtaattcatc ttttgtcatt tgtttgaatt
     6181 ctttacttga agcttcattc atcactttaa ttaagagata aatattgaag agaaataaca
     6241 caataagcgg aaggacaatt aggacgaaaa accatacgcg gttaccaatt aaccatttaa
     6301 taaatgggcc tcctgatcct gcttgaacat tctttaaagc gacaatagaa atggttacta
     6361 aagcgaccat cagcgctaat tctaaaccaa gtagcaaata tgttgcccaa cttggtaatt
     6421 taaatttctt tttcttttct tggccagtca tgaggtactc cttcacatgt attatttcac
     6481 acgtaataaa attttacatt aaatgtaatt aagtgtcaaa catatctatg cgattatatg
     6541 gctctttaac gataaatctt tataaaaata cgtgtttttt aataaaaaac atcaacccaa
     6601 ctggatggtg tgttttaata attcatgatt aatattaaga gcttatactc ctgtcgagta
     6661 aatatcaagt ttaattttaa acttaccgcc aataatgctt tcagcagcat gaatatcagt
     6721 accttcaatc cacacacgga atgtcacatc agcggtacca gtacctgctg gtaacactgc
     6781 aatttctgga taaactgctt gtgaatctgc aatagtgtag tgtgtctcaa acgaatcact
     6841 ttttactgtt gcgggcacta attcatgggt agtaagacca tacgctcccc cacttcctaa
     6901 ttgaagatat tcgggattta tttctgcttc tggtgcatat aagatatcaa cacttggcgt
     6961 gcctgtagct gagtcattgt tatcataact tattaccgcc atgcgtaatg cttttaatgc
     7021 accaacatct tttggattag ccggatccgc cggcagaatc gctgtgtttt gcccaagaaa
     7081 gacatataaa tttaatgggt cttcgccagg cgagcgagat aatgtaagag tgaaatcaat
     7141 aaaatatcca tccgcatcgc caggatttgt acttggtacg gtattaatga ctgctgcggt
     7201 tacattgtct gaattccagc gcggtttata gaagttgata ccattgcccg agatatctgt
     7261 aactttattc gtgccggtta atgttatttt agtgattccg tctagttcac tagtagcgga
     7321 taatgaattc aaagagctct tataggtaac cactaaatca gaatcccgca tgtaaacttc
     7381 agcatcacca taagtgatac ctgcagtacg cacagttgta aaccacgcta aggttccact
     7441 agttgcggca atagcgctaa taaataatag cgttaatact gttcctaatc ttttatttct
     7501 tttattcatt agttttattt ccttaatttt ttattgaggt ttgtaaaaag tttcacccca
     7561 gagagaatgt ccctggggcg aaacggggtc gcaactttaa ttagttaacg aatgtgtata
     7621 aagcaatgtt aacattgaac acaccaccga gaatgtcgtt atcacagtcg ctatcttcac
     7681 cttctaacca aatacggaag gtaacgtctc tttcattagc ttcattttta cttaagtcac
     7741 aaactaagaa accagcagct tc
//
LOCUS       DAOPCD010000022         7655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_8680_length_7655_cov_4.041974, whole genome shotgun sequence.
ACCESSION   DAOPCD010000022 DAOPCD010000000
VERSION     DAOPCD010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8680_length_7655_cov_4.041974"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLQ55_01265"
     CDS             <1..685
                     /locus_tag="PLQ55_01265"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPK28601.1"
                     /translation="ETVVARQPFARDAHIIGVSPSVKEIRNVVETDYVIVDLISIGFV
                     FIILLVSFRSISLPFILVLVIQISIWINMAIPYLTSQPMIFIGYMIVSSVQLGATIDY
                     AILFTGFYTEGRKTMIRRDAIKYALDSGGHSILTSCLILTSAGYSLKFLSSIEGVSSL
                     GELIGRGAALSGILVLVLLPQLLYIFDKVVEKTTWKMRFYKGGTKVGRVIIEDEGYSN
                     SDQDPTLLS"
     gene            776..1792
                     /locus_tag="PLQ55_01270"
     CDS             776..1792
                     /locus_tag="PLQ55_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HPK28602.1"
                     /translation="MIDDPQTWVLLILYFVAGMYFSSSETAFMTANRFKIRAKAEEGS
                     VSAKLAAWVINRLENSIIAVVVFNNLAHLLFTALLTVVLVNQYGNDTGELLATLISVP
                     LMFLFAETIPKVLGRRYSEQYALISAYLLVPFIIISYPVALFFRGIVWVVKKLFKATE
                     DNIFTADDFEQVIETIEEHGIINEDASDIIISSLEFSETVVKDVITPKNAIVAYDISK
                     FNKDSFKDFVLSTHFSRIPLYDGSLDKIVGVILVREYIKHYQKKPNINPRTIMARPYY
                     VNYKITLTKMVEGFKKNNTHIAFVVDDKKKLLGMVTMEDVLEELVGQIAETSGTVKGA
                     SVNG"
     gene            1785..3056
                     /locus_tag="PLQ55_01275"
     CDS             1785..3056
                     /locus_tag="PLQ55_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPK28603.1"
                     /translation="MGNVIRFAIVILLFALSFFFSSSESAFSTINRLRVERDVEDGKK
                     RSKTLLRIITNYENTVQTILLGNNFVNIAVTALVTGMALAYDNAHGATGQYVAIFSVV
                     LFIVLLTIGEIVPKTLAVKLNYQMSYAYANIFMFFYYLFTPLLFPFRLISARRKKKNG
                     NEEVNGDEIYTEDELQIIVDEIEEQGLIDEETSDLVRSALEFTETEAYEIMTPRVDVF
                     SYDIEDDIKELMKEEDIFKYSRIPVYEDTIDNIIGILRTKDLLKLSLSDKKINVKSLI
                     KPPLFVHHTKNVSQILTEFKQTKNHIAIVIDEYGGTEGIITMEDILEEIIGEIWDEAD
                     EVDEPIVKLEDNHYIIDGGLNIEDFFELFELSEDEDNDYDTVAGWCIEMLDRFAKAGD
                     EFTYEKITVKIIEVEGFVVEKVEVIVESEED"
     gene            3056..3961
                     /locus_tag="PLQ55_01280"
     CDS             3056..3961
                     /locus_tag="PLQ55_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK28604.1"
                     /translation="MYVEKFLERIPSLLNKTYVVTGANSGLGFALSTLLVSKGARVIM
                     ANRNPKRSEDAINKIRELYPAASVMVLPFDQSKKDSIEAFVNSLISHNIKPEGFVLNA
                     GIYSPAKDAKTEDGVALTFGINYLGNFIFTQTLANHGLISAETRLIYISSPAYSRKFS
                     SQILARAISGQKITRPKQYAVAKTAQKIFVLGLMTKSPLIPFENKGKIYLYHPGIAVS
                     NIARFKWKFFNKIVHRFMSVFFHPPEKAVLGALVALTTDKDLNGKILVPRGFLEINGI
                     PRIKKIDEKLLSHLPYLITETLKLI"
     gene            complement(3954..4496)
                     /locus_tag="PLQ55_01285"
     CDS             complement(3954..4496)
                     /locus_tag="PLQ55_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPK28605.1"
                     /translation="MIVLMGASASGKTEVAKMLGKLFSIKKVVTHTTREMRVNEVNGR
                     DYHFVTKEEFLRLKAEDYFVETVEYNDNFYGTSKAEIAMNKVLIIEPIGLKSIRALNS
                     EHIVVFFMQASRAIRRKRMINRGDNPKIATERILIDDAKFNPKNIEGIDYTIDSENQS
                     IKEIAIEVYEKYALKLVNFK"
     gene            4621..5565
                     /locus_tag="PLQ55_01290"
     CDS             4621..5565
                     /locus_tag="PLQ55_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28606.1"
                     /translation="MSVFIPLVPESNFQTSALGSYRNNPGDITQSYEVAIPFIASYTT
                     TLTYRYTVYNSANKIVYTQPSTSTRALRNVRVLITYRATAGKFTVGQNRIILYYKTSS
                     EAERRHYSYFYGYQKGVNVNLNNGSAARQLLEAEVCYLYDSTNRSDIFRKMSFSSANL
                     TPIKHFNHDLYFDFSGLFLSIGVLYDNENFYSSAALYCSNPALFPYLANVGGRAMQYL
                     NLTRAYNRLVTSVKTPLFVRADTLQIARSKYSAEYLNTDTYFFPKYHFDELQNTKFTL
                     EIKDFSYSKFNLKYEFTIQIDHPYFGTSGEHEVIINRY"
     gene            5567..5932
                     /locus_tag="PLQ55_01295"
     CDS             5567..5932
                     /locus_tag="PLQ55_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28607.1"
                     /translation="MAWSNFAFLFLFTYSTLLAINIRYHHVNEVLSSVTHDHIYHVTT
                     MDLVTYELYFEKPLVRVVVANHFNLNMKRENYEYTITFYTNGVVSEESERSRYFTINL
                     VCPLGFGVTFDKTFAYYVS"
     gene            5935..6861
                     /locus_tag="PLQ55_01300"
     CDS             5935..6861
                     /locus_tag="PLQ55_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPK28608.1"
                     /translation="MEKVIKVIEESFLAPLLSDKNITDISYNGHKLFYFSNESGRKLY
                     GEINHKTVASFLRNVANFANERFSYADTFLDISIGKYRLSAMHYACSRFEFEESLSFA
                     LRINHNFLASNPHYLNNDVVDVLKYVMKENRSIIISGTTGVGKTTLQKYLLMLLPANT
                     RVLVIDNVLELSEVTTLNKDLDLTLWQSLTNKEEEIKSFIQKALRFHPDYLIIAESRG
                     GEMKEILQGAISGHPNIVTLHADSTALAYERVIYLAKHDDRHSLLQAFPFVIHLAKKY
                     KADGSIYRYVEKITEYNVDTDEIEILYHEVIP"
     gene            6858..7547
                     /locus_tag="PLQ55_01305"
     CDS             6858..7547
                     /locus_tag="PLQ55_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28609.1"
                     /translation="MIIIYGGAFFLVIVIAIFSALVLGGIKITIINALIALVVAAFLT
                     YRVTNYRKDIEKRRFMFNFMEYFILNFDIQKTVEATLTTIYPLLDVKKSRVYLTMNED
                     GMLLLEKLRLTFAHQYYESFLEMVKLINEHGGEMLKVAEVLLFSISNSETQLIKLTRI
                     DNAYLIKFIFNWFFIMLVAIVFRLALDGFLKFETLPLIYVAGMEVFIAIFLTSIVLVF
                     ENRIRRTRRVS"
BASE COUNT         2410 a         1190 c         1455 g         2600 t
ORIGIN      
        1 tgaaactgtt gtcgcccgtc aaccttttgc ccgtgatgca catattattg gtgtctcgcc
       61 atcagttaaa gaaattcgta atgttgtcga gactgactat gtgattgtag acttaatttc
      121 aattggcttt gtatttatca tattacttgt tagttttcgc agtatctcat tgccatttat
      181 tttagtgctt gttattcaaa tttcgatttg gattaatatg gcaattccgt atttaacgag
      241 tcaaccgatg atttttattg gttatatgat tgtaagttcg gtacaactcg gggcgacgat
      301 tgattacgct atacttttta ctggatttta cactgaggga cgaaaaacga tgattagacg
      361 tgatgcaatt aagtatgccc ttgatagcgg gggtcatagt attttaacat cgtgcttaat
      421 tttgacctct gctggttata gtctaaaatt cctctcaagt attgaaggcg tgtcctcgct
      481 tggtgaacta ataggccgtg gtgcagcact tagtggtatc cttgtgcttg tgttattacc
      541 acagttgcta tatatcttcg ataaagttgt ggaaaaaaca acatggaaga tgcgctttta
      601 taagggcggg acgaaagttg gacgggttat tattgaagat gaaggttata gtaatagtga
      661 tcaagaccca accttgctta gttaacttta gcgttgcaca aatttcggcg ctgtgataac
      721 atgctatcgt tgcatatgaa tatgcacatt tataaagaag gagtgtggtt tctaaatgat
      781 tgatgaccct cagacgtggg ttctcttaat attatatttt gtcgctggga tgtactttag
      841 ttcatctgaa acggcgttta tgactgcgaa ccgctttaaa ataagagcca aagcagaaga
      901 aggtagcgtg agcgctaaac ttgctgcttg ggttataaat agactagaaa attcaattat
      961 tgctgtcgtt gtgtttaata atttagcgca cttacttttt actgcgcttt taactgttgt
     1021 ccttgttaat cagtacggca atgacactgg tgaattactt gcaactttaa ttagtgtgcc
     1081 gctgatgttt ttatttgcgg aaacgattcc aaaagtatta ggacgtcgct atagtgaaca
     1141 atacgcatta attagcgctt atcttttagt gccatttatt attattagct atcctgtcgc
     1201 gctttttttc cgtggtattg tctgggttgt taaaaaactt ttcaaagcca cagaagataa
     1261 tattttcacc gctgatgatt ttgagcaagt cattgaaaca attgaagagc acgggattat
     1321 taatgaagac gctagtgata ttattatctc ctcccttgaa tttagtgaaa cagtggtaaa
     1381 agatgttatc acaccgaaaa acgcgattgt tgcctatgac attagtaaat ttaataaaga
     1441 ttcatttaaa gatttcgtgc tatcaacaca tttttcaaga attccgctgt atgacggttc
     1501 acttgataaa attgttggcg ttattttagt gcgggaatat attaaacatt atcaaaagaa
     1561 accaaatatt aacccacgca caattatggc acgcccatat tatgttaatt ataagatcac
     1621 gcttactaaa atggttgaag gctttaagaa gaacaacacg catattgctt ttgtcgtcga
     1681 tgataaaaag aaactacttg gtatggttac aatggaagac gtccttgaag aattagtcgg
     1741 ccaaattgcg gaaacaagtg gtactgttaa aggagcaagc gttaatgggt aatgtaatta
     1801 gatttgcaat tgttattctt ttatttgcgc ttagtttctt tttctcgagt tcagagagtg
     1861 ctttttccac gattaatcgg cttcgtgtgg aacgtgatgt ggaagacggt aaaaagcgta
     1921 gcaaaacact gctgagaata attacgaatt atgagaatac tgtccaaaca atattacttg
     1981 gtaacaattt tgtaaatatt gcagttaccg cccttgtgac agggatggcg ctagcctatg
     2041 ataatgcgca tggcgctact ggacaatatg tagctatttt ctctgttgtt ttatttattg
     2101 tccttttaac gattggtgaa attgtgccaa aaacactcgc tgttaaatta aattatcaaa
     2161 tgtcgtatgc ttacgcgaat atctttatgt tcttttatta tttatttacg ccgcttttat
     2221 ttccgtttag actaattagt gcacgccgga agaaaaagaa tggtaatgaa gaagtaaatg
     2281 gcgatgaaat ttatacggaa gacgaattac aaattattgt cgatgaaatt gaagagcaag
     2341 gcttaattga cgaagagaca agtgacttag tgcgcagtgc gcttgaattt acggaaacag
     2401 aggcttacga aattatgacg ccgcgtgttg atgtctttag ttatgatatt gaagatgaca
     2461 ttaaagagtt aatgaaagaa gaagatattt ttaaatatag tcgtatcccc gtttacgaag
     2521 atacaatcga taatattatt ggtattttac ggacaaaaga cttacttaaa ctttcacttt
     2581 ccgataagaa aattaatgtg aaaagcttaa ttaaaccgcc acttttcgtc catcatacga
     2641 aaaatgttag tcaaatttta accgaattca agcaaacgaa aaaccatatt gccattgtca
     2701 ttgatgagta tggtggcacc gagggtatta ttacaatgga agacatcctt gaagaaatta
     2761 ttggtgaaat ttgggatgag gctgatgaag ttgatgagcc gattgttaag cttgaagata
     2821 atcattacat tattgatggt ggcttaaata ttgaagactt ctttgaatta tttgaattaa
     2881 gcgaagatga agataatgat tatgatacgg tcgctggttg gtgtattgaa atgctcgatc
     2941 gttttgctaa ggcgggcgac gaattcactt atgaaaaaat aactgtcaaa atcattgaag
     3001 ttgaaggctt tgttgttgaa aaagttgaag ttatcgttga aagtgaagaa gattaatgta
     3061 cgttgaaaag tttttagaac gaatcccgtc tttattaaat aaaacttatg tcgttactgg
     3121 cgctaatagt ggtttaggtt ttgcccttag cacactcctt gtaagtaaag gggcacgcgt
     3181 tattatggct aatcgtaatc ctaaacggag tgaagacgca ataaataaga tacgcgaatt
     3241 atatcctgct gcgagtgtaa tggtactccc ttttgatcaa agtaaaaagg attctattga
     3301 agcctttgtt aatagtttaa ttagtcacaa tattaagcct gagggctttg tgcttaatgc
     3361 tggcatttat tcgcctgcta aagatgctaa gactgaagat ggtgttgcgc ttacattcgg
     3421 tattaattac ttaggaaact ttatttttac acaaacactt gcaaatcacg ggttaattag
     3481 tgcagaaaca aggttaattt atatttctag tccagcatat tcgcgtaagt ttagctcgca
     3541 aatacttgca cgggcaataa gcggacaaaa gatcacgcgg ccgaaacaat atgctgttgc
     3601 aaaaactgcc caaaaaatat ttgtgcttgg cttaatgact aagtctccgc taattccgtt
     3661 tgaaaataaa ggtaaaattt atctttatca cccaggaata gctgttagta atatcgcacg
     3721 cttcaaatgg aaattcttta acaagattgt ccacagattt atgagtgtgt tttttcatcc
     3781 tcccgaaaaa gccgtccttg gtgcccttgt ggcgctaacg acagataaag atttaaatgg
     3841 gaaaatttta gtgccgcgtg ggttccttga aattaacggg attccgcgta ttaaaaaaat
     3901 agacgagaaa cttttatctc atctacctta cttaataaca gaaacattaa agcttattta
     3961 aaattaacga gttttaaggc atatttttca tatacttcaa tcgcaatttc ttttatgctt
     4021 tgattttcac tatcaatcgt ataatcgata ccttcgatgt tttttggatt aaatttagcg
     4081 tcatcgatta aaatacgttc agtggcaatt tttggattat caccgcgatt aatcatccgt
     4141 ttacggcgaa tagctctact agcttgcatg aaaaagacga cgatatgttc gctatttaag
     4201 gctcttattg attttaaacc aataggttca ataattaata ctttattcat ggcgatttca
     4261 gcttttgaag ttccgtagaa gttatcatta tactctacag tctctacaaa gtagtcttca
     4321 gctttaagtc gtaaaaattc ttcttttgtg acaaagtgat aatcgcgacc attaacttcg
     4381 ttaacacgca tttcacgagt tgtgtgcgtc acaacttttt taatactaaa aagtttgcca
     4441 agcattttgg caacttccgt ctttccgctt gcgcttgctc ccattagtac aatcatacat
     4501 aaataataaa ataaaatttt gttaattgca cgaagaatca gtcgtaaatg tgaaaaagtt
     4561 caaaatactc ttttatttat taccatgaga tactttaaaa cattgtggtt aacgattttt
     4621 atgagcgtgt ttattccgct tgtacctgaa agtaattttc aaacttcagc gcttggtagt
     4681 tatcgtaata atcctggaga tattacacag agttatgaag tggcgattcc ttttattgct
     4741 tcttacacga caacattaac ataccgctat accgtataca atagcgctaa taagattgtt
     4801 tatactcaac catcaacatc gacaagggca ctgcgtaatg ttcgggtgct aatcacatat
     4861 cgtgctacag caggaaagtt tacggttggt caaaatcgta ttattttata ttacaaaaca
     4921 tcaagtgaag cagagcgtcg tcattattca tatttttatg gctatcaaaa aggtgtcaat
     4981 gttaatttaa ataatggtag tgcggcacgg caattattag aagctgaagt atgttattta
     5041 tatgatagta ctaatcgcag tgatatattt cggaagatga gttttagctc cgctaattta
     5101 acgcctatta aacattttaa tcatgactta tattttgatt ttagtgggct ttttttatca
     5161 attggggtgc tttatgataa cgaaaatttt tatagcagtg cggctttata ttgtagcaac
     5221 ccagctctct ttccttatct tgctaatgtt ggcggaagag caatgcagta tttaaactta
     5281 acacgtgctt ataatcgttt agtaacgagt gtgaaaacac cattgtttgt gagagcagat
     5341 acactacaaa ttgcgcgtag taaatatagt gccgaatatc taaataccga tacatatttc
     5401 tttcctaagt atcattttga tgagttacaa aacacgaagt ttacattaga gatcaaagat
     5461 tttagttata gtaaatttaa tctaaaatac gaatttacca ttcaaataga tcacccttac
     5521 tttggcacaa gcggtgaaca tgaagtaata attaataggt actagtatgg catggagtaa
     5581 ttttgctttt ttatttttat ttacttatag cacactgcta gcgataaata ttcgctatca
     5641 ccatgttaat gaagtactta gttctgtaac tcatgatcat atttatcatg tgacaacaat
     5701 ggatttagtt acttatgaac tctactttga aaaaccgctt gtaagagttg tagtcgctaa
     5761 tcattttaac cttaatatga aaagagaaaa ttacgaatat acgattacat tttacacaaa
     5821 tggagtcgtt agtgaagaaa gtgaaagaag tcgatatttt acaattaacc tcgtgtgtcc
     5881 actaggcttt ggtgtgactt ttgataaaac atttgcctat tatgtgagtt aacaatggaa
     5941 aaagtaatta aagtaattga agagagtttt ttagccccgc tccttagtga taaaaacata
     6001 acagatatta gttataacgg tcacaaatta ttttactttt caaatgagag tggtcgcaag
     6061 ttatatggcg aaattaatca taagactgtc gcaagctttt tacgtaatgt cgctaatttt
     6121 gctaatgaga gattttctta cgctgataca tttcttgata tatcaattgg aaagtatcgc
     6181 cttagtgcga tgcattatgc atgtagtaga tttgaatttg aagaaagttt aagttttgcc
     6241 ttgcgcatta atcataattt tctcgcaagt aatccgcatt atttaaataa tgatgttgtt
     6301 gatgttctaa agtatgtgat gaaagaaaat cgctccatca tcattagcgg cacaactgga
     6361 gtcggtaaaa cgactttaca aaagtattta ttaatgcttt tacccgctaa tacgcgggtg
     6421 ttagtgattg ataatgtcct tgaattaagt gaagtcacaa cattaaataa agatttagat
     6481 ttaacacttt ggcaatcatt aacaaataaa gaagaagaaa ttaaaagttt tatccaaaag
     6541 gctttacggt ttcatcccga ttatttaatt attgccgaga gtcggggtgg tgagatgaaa
     6601 gaaatactgc aaggagcaat aagcggtcat ccaaatattg taacactcca cgctgatagt
     6661 acagctttag catacgaacg cgtcatctat ttagcaaagc atgatgatcg tcatagttta
     6721 ttacaagctt ttccgtttgt tattcattta gcaaaaaaat ataaggcaga tggctcgatt
     6781 tatcgttatg tcgaaaaaat cactgaatac aatgttgaca ctgatgaaat tgaaatactt
     6841 tatcacgagg tgattccgtg attattattt atggtggtgc tttctttttg gttatagtaa
     6901 tagcaatatt tagtgcccta gtgctaggcg gtataaaaat aacgattatc aatgcactaa
     6961 ttgcactagt tgtcgctgct tttcttacat atcgcgttac taattatcgc aaagacattg
     7021 aaaaaagacg ctttatgttt aattttatgg aatattttat tcttaacttt gacattcaaa
     7081 aaacagtaga agcaacacta actacaatat atccactgct tgacgtaaaa aaatcaagag
     7141 tatatttaac aatgaatgaa gacgggatgc ttttacttga gaaactgcga cttacttttg
     7201 ctcatcaata ttatgaatca tttcttgaga tggtgaaact tattaatgaa cacggtggtg
     7261 aaatgcttaa agtagcggaa gtactgcttt ttagtatttc taatagtgaa acgcaactta
     7321 ttaaattaac tagaattgat aatgcttatc ttattaaatt tatttttaat tggtttttta
     7381 ttatgttagt agcaatcgtc ttccgcctag cgcttgatgg ctttttaaaa tttgaaacat
     7441 taccactaat ttatgttgcg ggtatggaag tctttatcgc tatattttta acaagcattg
     7501 ttttagtatt tgaaaataga atcaggagaa cacgccgtgt tagttaaatg gttagaacgt
     7561 agtgccgggt tattattaat catggttggc gctattttat attctggaag aaaaacttaa
     7621 cacttgcact gatatttgct ggtgttggtt ttaca
//
LOCUS       DAOPCD010000023         7645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_8695_length_7645_cov_4.314229, whole genome shotgun sequence.
ACCESSION   DAOPCD010000023 DAOPCD010000000
VERSION     DAOPCD010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8695_length_7645_cov_4.314229"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PLQ55_01310"
     CDS             complement(<1..1149)
                     /locus_tag="PLQ55_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28610.1"
                     /translation="MKKRRLLIPLIGLALLLTGCKTTSSEEKPVSSEQPPVTSVTTEQ
                     PSVSSEEPPASSEETPVANIKFGADPFNGTSATGVAGVGVALYYDATEDDVAVAFDKV
                     AFTVNKPSGVLFDPVIGLVEKLSVYYLRPMEPGTYTVTLTVEDSAGLKATATKEIVVS
                     TGDLSGDVDAIKAAIAAIYPDNIYTNLDYRTSEGVFNEWVVVGKNFTVFDREDYTGNY
                     ATAFIPFARFEGGEALQDFTISFKYTTLNEVWKLLFSFWTGEVGADNFAGDYLRLLTN
                     RNAIGIQGDAQQASEFQDEGEATGIPLKEGPVWIKFTRQVTKVGEGDYTVVFKLFTST
                     DGETFVNNTTATLVNQVNTAGGTAGLLTGFLPFSIDNDFIVEDLAVSGT"
     gene            complement(1189..2646)
                     /locus_tag="PLQ55_01315"
     CDS             complement(1189..2646)
                     /locus_tag="PLQ55_01315"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HPK28611.1"
                     /translation="MFCYHKTSASNNEKFFRKGIFLSITLLIATTVLTSCKGGTSVNS
                     EIRSSEFNNNEVVYPSNPAFGNNVTAHDPSIFKDDDGTYYTFGSHFAVTKSINLMQWT
                     QVANDGGANVLYARNWRTVLADAFAHVGERPGSTWAPCVHKFGEKYYMYYSLSTFGSS
                     VSYIGRVEGDSVLGPYDNSVEIVKSDGRGGPNAIDPELFFSKDGRLWLVYGSFFAGIY
                     IKELYASGENIGLPKEDGYGKLLWQGGDQGPEGPFIFYNPNTDYFYLMASHGSLSTNY
                     NMRVARSKNPDGPYTDPRGMSAENYRNSGLKLAGNYQFQGYPIGYAALGHNSILREDG
                     KYYVVYHSRYRSGASGVTGYHNQFVNQMFFNKNGWPVLAPNRYAGEELQVITMAQASK
                     DYDVLIHTGGDSEGFATSVTYKFSQNGDLLDAEEDAIGTWSINEDYYVTIKIGLNTYE
                     GVIIPQWNNDLQRAGLSITAYSRNGLSLWANEHFR"
     gene            2931..3938
                     /locus_tag="PLQ55_01320"
     CDS             2931..3938
                     /locus_tag="PLQ55_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK28612.1"
                     /translation="MPTIRDIARKANVSPATVSRVLNGDPTINVQAKTKRRIFEVAED
                     LAYTKHLQPKKNQQKNKFRIAIYHWYTIEQEINDPYFISIRIGVQRACRELGIAFDVI
                     YREDRITDPFEDQKYDGVIMIGSFTRETQTFISDRFSAVVYANSEDEDGKCDCVRVDF
                     RALTHDVLHLLINKGHKKIAYIGGRERVSSEPNKTPDPRELEYIEVMHQNKLYYKPYV
                     KVGEYSAHSGYELANELIDNNEGNTPTAIFCGNDSIALGVSKAIQERGFKIPDDIAIF
                     GVNDIPTLQFTSPSLSSVKIHTEFMGELALKLLNERMKKERSLKLSVNVPYELILRES
                     A"
     gene            complement(3940..5358)
                     /locus_tag="PLQ55_01325"
     CDS             complement(3940..5358)
                     /locus_tag="PLQ55_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HPK28613.1"
                     /translation="MKETNKKTRLKGFMKTTVLSFQHMFAMLGATVLVPMLAGLSISI
                     TLITAGIGTIIFYFVTKRKVPVFLGSSFAYLPALMSIMAESGEVGSLAWQQNMGKLVI
                     AIFFTGVMYLIFAGVIRLIGVEKVRKMFPPRVVGPVIIVLGMLLVPSIFWNNIIAPYA
                     LYGAEAWKVWTTALITLLSIIAISAFAKPKSFFKAAPILLGFLIGYVYGAIVGLVDFS
                     TLKGSDIVIFQSIGSLLGFYKYLKFDWNAILMMVPIAFVTLMEHIGDISANSTVCGKD
                     FFVDPGIDCTLAGDGFAIMAASALGGPPQTTYGENTAILVITENYEPRNVFYAAILAV
                     IFGVISIFGNVIATIPSAVIGGASIILFGMISASGLRMVIEHKADIGQTKDLIVVSVT
                     LAVGLGLGALSVASDILSAVGVTTFDPRLLKVMIGNVELSPLAIATLVAVLLNLVIPN
                     PKVVKSEEKKASTEDTLTNEIN"
     gene            complement(5400..5900)
                     /locus_tag="PLQ55_01330"
     CDS             complement(5400..5900)
                     /locus_tag="PLQ55_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPK28614.1"
                     /translation="MEKWDLYTKDEKKTGKIVDKGALIPEGYYHIIVETLIKHTDGTY
                     LVMQRSYDKKEYPGKYEGSCGGSIKAGETKEEGAIREVFEESGLVINEVTPTYYFIQK
                     DVPAINQGYLAIISAKDKTIKYQKEETINHKWLTLKELKAFIKSDEYNESHASRLVTY
                     LDSLNE"
     gene            complement(6067..7140)
                     /locus_tag="PLQ55_01335"
     CDS             complement(6067..7140)
                     /locus_tag="PLQ55_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28615.1"
                     /translation="MKRKRMLWLPLLALLLGGCSVDFDFGPTSKDSEGGGGGTTSQTG
                     GAVSRDEANSRVTAAFTNSDIADATTYQQTTLQEVTDDTKEYVNDALKYHVVENTNSN
                     VVVKATGLQTDDPVAKVTGEPLEDMTEDGVETYKLIDNREFYYASEWIYYHNATRKRY
                     GNPEEPFIDTEVKLKQHMGPTNGQQLVEGLEGFDLGDAPVPTMFIGSELDVLQQTANI
                     TATKNGRNITVIYSINKDAFIAMMESGLRGILFSEEMTSEERPEAEEFLQWYVALLEE
                     VLDLREYKITIGADGNDHLTQVRADFELITTMVYEPSETYPKGYTQVGKLSGFLDMKP
                     KFNEEFTITLPDFSDYEEGIIFE"
     assembly_gap    7369..7378
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7457..>7645)
                     /locus_tag="PLQ55_01340"
     CDS             complement(7457..>7645)
                     /locus_tag="PLQ55_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28616.1"
                     /translation="VEKIRTDIDMVTNKYNEEVEPKVLVASEELKTFTESTMEINKKL
                     TIKYPNIDDYPEAQPIIR"
BASE COUNT         2356 a         1560 c         1235 g         2484 t
ORIGIN      
        1 tgttccactc acagctagat cttcaacgat aaaatcattg tcgattgaga atggaaggaa
       61 tccagttaat agaccagcag tgccaccagc ggtattaact tggttaacta atgttgcggt
      121 agtattatta acaaatgttt caccatcggt tgaagtaaag agtttgaaaa caacagtgta
      181 atcaccttca ccgactttag taacttgacg ggtgaattta atccaaactg gtccttcttt
      241 taatggaata ccagttgctt caccttcatc ttggaattct gaagcttgtt gagcatcacc
      301 ttgaatacca attgcattac gatttgttaa tagacggaga tagtcacctg caaaattatc
      361 agcacctact tcaccggtcc agaaactaaa taataatttc caaacctcat taagtgtggt
      421 gtatttgaaa gaaatggtga aatcttgtaa tgcttcacca ccttcaaagc gggcaaatgg
      481 aataaatgct gttgcatagt taccagtata atcttcgcga tcaaatacgg taaagttttt
      541 gccaacaacg acccattcat tgaagacacc ttcactagtg cgatagtcaa ggtttgtata
      601 aatgttatct ggataaatcg cagcaatagc cgctttaatt gcatcaacat cacctgataa
      661 atcaccggtt gaaacaacaa tttctttagt agcagttgct tttaatcctg cgctatcttc
      721 aactgtcagt gttaccgtat atgttcccgg ttccattggt cgtaaataat aaacactaag
      781 tttttccact aaaccaatta ctggatcaaa gagaacacct gatggtttat taacagtaaa
      841 tgccactttg tcaaaagcga cagcgacatc gtcttcagtt gcgtcgtaat aaagcgcaac
      901 accaacgccc gcgacacctg ttgcggaagt tccgttgaac gggtcagcac caaatttaat
      961 gttagcaacc ggcgtttctt ccgaacttgc tggtggttct tcacttgaaa ccgatggttg
     1021 ttctgtagta acgcttgtta ctggtggctg ttccgaactt actggttttt cttcacttga
     1081 tgttgtctta catcctgtaa gcagaagtgc taaaccaata agtggaataa gtagtcttct
     1141 ttttttcata ttattctcct tttttattag ccttttttgg ctttataatt atctaaaatg
     1201 ttcgttggcc cataaactta ggccattgcg gctataagcc gtaattgaaa gtccggctcg
     1261 ttgaagatcg ttattccatt gcggaataat gacaccttca tatgtattta agccaatttt
     1321 aatcgtgaca taataatctt cgttaatgct ccatgtaccg atagcatctt cctcagcatc
     1381 tagcaagtca ccgttttggc taaacttata agttactgat gtagcaaacc cttcactatc
     1441 accccctgta tgtattaata catcataatc ttttgacgct tgtgccattg taataacttg
     1501 aagttcttcg cccgcatagc gatttggtgc aagtaccggc caaccatttt tgttaaaaaa
     1561 catttgattg acaaattgat tatgataacc cgtcacacct gatgcgccac tacgataacg
     1621 cgaatgataa acaacataat attttccgtc ttctcttaaa atcgaattat gaccgagtgc
     1681 agcataacct attggataac cttgaaattg ataattacca gcgagtttta gacctgaatt
     1741 acgataattt tcagcactca taccgcgcgg gtcagtataa ggaccgtctg ggttcttact
     1801 gcgcgcaaca cgcatattat aattagtaga taaggaacca tgcgatgcca ttaaatagaa
     1861 ataatcagtg ttaggattat agaaaatgaa aggaccttct gggccttgat caccaccttg
     1921 ccataaaagc ttgccgtagc catcttcttt tggtaaccca atattttcgc cgcttgcata
     1981 taattcttta atataaatac ccgcaaaaaa tgaaccataa actaaccata atcttccgtc
     2041 tttactaaag aataattcag gatcaattgc atttggtcca ccgcgaccat cagattttac
     2101 aatttccaca gaattgtcgt atggaccaag cacactatct ccttcaacac gaccaatata
     2161 agaaacacta ctaccaaaag ttgataaaga atagtacata taatattttt caccaaactt
     2221 atgaacacaa ggtgcccaag tactaccggg tctttcacca acatgagcaa aagcgtccgc
     2281 taatacagtg cgccagttac gagcatataa aacattagcg ccaccatcat tagcaacttg
     2341 ggtccattgc attaagttta ttgatttagt aacagcaaaa tgagaaccaa aagtataata
     2401 ggtcccatca tcatccttaa agattgaagg atcatgtgcc gtaacattat taccaaaggc
     2461 aggattagac ggataaacta cttcattatt attaaattca ctactccgta tttcgctatt
     2521 cacgcttgta cctcccttac acgatgttaa aactgttgtc gctattaaaa gagttatcga
     2581 taggaaaata cctttacgaa aaaacttctc attattacta gcgctagttt tatggtaaca
     2641 aaacatagat tcctccttag gttcatctaa tcgttatgaa atatgtggta attatcttac
     2701 ctacagatta tcacatgttt actaaatgtc aagcgtaatt tatttctggc taaaacgaaa
     2761 atttgcgttg taatttcttt gttttttaac atagttcata taaaatcaat gttatttgtt
     2821 gcgcagttta aaattgttta ctgtttgttt agtaaatatt ttaatttaat tgttgatatt
     2881 taccagacct tataataaaa tgaaactaat taatatgaaa tgaggtgctt atgccaacca
     2941 ttcgtgatat tgctagaaaa gctaatgttt cacccgcaac tgtttctcgt gtcttaaacg
     3001 gcgacccaac tatcaatgtt caagctaaaa caaaaagaag aatttttgaa gttgccgaag
     3061 acttagccta cactaaacat ttgcaaccaa agaaaaatca acaaaaaaat aaattccgta
     3121 ttgctattta tcactggtat acaatcgaac aagaaattaa tgatccttat tttatttcaa
     3181 ttagaattgg cgtccaacga gcatgtcgtg aacttggcat tgcttttgat gtaatttatc
     3241 gtgaagatcg catcacagat ccttttgaag atcagaaata cgatggtgtg attatgattg
     3301 gtagttttac gagagagaca caaacattta tttctgatcg cttttcggct gttgtgtacg
     3361 ctaattcgga agacgaggat ggtaaatgcg attgtgttcg cgttgatttc cgcgcactaa
     3421 cacacgatgt tcttcattta ctaatcaata aaggtcataa gaaaattgct tatattggtg
     3481 gccgcgagcg cgtttcttct gaaccaaaca aaacacctga tccgcgtgag ttagaatata
     3541 ttgaagtaat gcatcaaaac aagttatatt ataaacctta tgttaaggtt ggtgaatata
     3601 gcgctcatag tggttatgaa ctcgcaaacg aactaattga caataatgaa ggcaacacac
     3661 caacagcgat attctgcggc aatgactcga ttgctttagg tgtttcaaaa gctatccagg
     3721 aacgcggttt taaaatacca gacgatattg ctatatttgg tgttaatgat attcctaccc
     3781 tgcaatttac ttctccttca ttatcatcag ttaaaatcca cactgaattt atgggcgaat
     3841 tagcgcttaa actactaaac gaacgcatga aaaaagaacg ttcactaaaa ttaagtgtaa
     3901 acgttcctta tgaactcatt ttacgcgaaa gtgcttaaat taatttattt catttgttaa
     3961 ggtatcttct gttgatgcct ttttttcttc acttttaacg accttcggat taggaataac
     4021 taggttaagt aataccgcta caagcgtggc aattgctaag ggagataatt caacatttcc
     4081 aatcattact ttaagtaaac gcgggtcaaa agttgttaca ccaaccgcgc taaggatatc
     4141 acttgcgaca ctaagcgcac ctagtcctaa cccaaccgct aaagtaacac ttacaacgat
     4201 taagtctttt gtttggccaa tatcagcttt atgttcaatg accatccgta aacctgaagc
     4261 actaatcatt ccaaagagaa taattgatgc cccaccaata accgcacttg gaatagtagc
     4321 gattacatta ccaaaaatgc taatcacacc aaagataacc gctaaaattg cagcataaaa
     4381 cacattacgc ggttcatagt tttccgtaat tactaaaata gcagtatttt caccatatgt
     4441 tgtttgcggt ggtccgccta aagcactagc ggccataatc gcaaaaccgt cgcctgctaa
     4501 agtgcaatca ataccaggat caacaaaaaa gtctttgccg catactgtac tattagcgct
     4561 tatgtctcca atatgttcca tcagtgttac aaaagcaatt ggaaccatca taagaatagc
     4621 gttccaatca aactttaaat atttataaaa gcctagaagc gaaccaatac tttgaaatat
     4681 gacaatatca ctacctttga gtgtgctaaa gtcgactaac ccaactattg ctccgtatac
     4741 ataaccaatc aaaaagccaa gtaaaattgg cgctgcttta aaaaatgatt tgggcttagc
     4801 aaaagcgcta atggcaataa tacttaagag tgtaataagc gcagtcgtcc acaccttcca
     4861 cgcctcagcg ccataaagcg catatggggc aatgatatta ttccaaaaga ttgaaggtac
     4921 aagtaacatc cctaacacaa taatcacagg accgacgaca cgcggcggaa acatttttcg
     4981 caccttttca acaccaatta ggcgaattac tccggcaaaa atgaggtaca ttacaccagt
     5041 aaagaaaata gcaattacta gtttgcccat attttgttgc cacgcaagac tccccacttc
     5101 gccactttcc gccataatac tcatcagtgc tggcaagtaa gcgaaacttg aaccaagaaa
     5161 tactggtact ttcctttttg tcacaaaata gaaaatgatt gtcccaatcc ctgcggtaat
     5221 aagggtaata gaaattgata gacccgctag cattggcaca agcacagtcg caccgagcat
     5281 cgcaaacata tgctgaaaac ttaggactgt tgttttcata aaccctttga gacgggtttt
     5341 cttattagtt tccttcataa taatttcttc cttttagtta ttaatcatta ttctaccatt
     5401 tattcattta gtgaatctaa gtatgttact aacctacttg cgtgactttc attatattca
     5461 tcacttttaa taaatgcttt taattctttt agtgttaacc atttatgatt aattgtttct
     5521 tctttttgat atttaattgt tttatctttc gcgcttataa tcgctaaata cccttgatta
     5581 attgctggta catctttttg gataaagtaa taagttggtg ttacttcgtt aatgactaat
     5641 cctgattctt caaatacttc acggattgct ccttcttctt ttgtttcacc agccttaatt
     5701 gaaccgccac agcttccttc atatttcccg ggatattctt ttttatcata ggagcgttgc
     5761 atcactaaat atgtaccatc tgtgtgctta attaatgttt caacaataat atgataatag
     5821 ccttcaggta ttaatgcgcc tttatcaaca attttccctg ttttcttttc atcctttgta
     5881 tataaatccc atttttccat gacataacac ctttaattat tcgtaattaa aactattgta
     5941 tcaaaatata tttattttct taaagactaa tctaagtgaa tcgtgtttca ctaaatattt
     6001 ttataattat aaaatcaaaa gaaagagccg catttgcggc tccctccatt tagcttattt
     6061 tagaatttat tcaaatatta tgccttcttc ataatcacta aagtctggta atgtgatagt
     6121 gaactcttca ttgaattttg gtttcatatc aaggaaacca ctcaatttgc caacttgtgt
     6181 atatcctttc ggatatgttt cacttggttc ataaaccatt gttgtaatta attcaaagtc
     6241 cgcgcgcact tgcgttaagt gatcattacc atctgcgcca attgtaatct tgtactctct
     6301 gagatcgagc acttcttcta ggagcgcaac ataccactgc agaaattctt cggcttctgg
     6361 ccgttcttca cttgtcattt cttcactaaa gagaattccg cgtaagccgc tttccatcat
     6421 cgcaataaac gcatctttgt ttatgctata gatgacagtg atatttctcc catttttcgt
     6481 cgcagtaata tttgctgttt gttgtaaaac atctaattct gagccaataa acattgtcgg
     6541 cactggtgcg tcacctaaat caaacccttc aagtccttcc actagttgtt ggccatttgt
     6601 tggacccata tgttgtttta gttttacttc agtgtcaata aacggttctt ctgggttacc
     6661 ataacgctta cgcgtagcat tatgataata aatccattca ctagcgtaat aaaactcacg
     6721 attatcaata agcttatatg tttcaacacc gtcttcagtc atgtcttcaa gtggttcacc
     6781 cgttaccttc gctactggat catcagtttg tagtcctgtt gctttaacta ccacattaga
     6841 gttcgtattt tcaactacat gatacttaag agcgtcgtta acatattctt tagtatcatc
     6901 agttacttct tgtaatgttg tttgctgata tgttgtagca tcagcgatat ctgaattagt
     6961 aaatgccgcg gtaacacggc tattagcttc gtctctactg acggctccac ctgtttgtga
     7021 ggttgtgcct ccgcctcctc cttctgaatc ttttgatgtt ggtccaaaat caaaatcgac
     7081 cgaacatccg ccaagaagta gggctaaaag tggtagccat aacattcttt ttcttttcat
     7141 agcaaagtac ctacttacct attgctacac cttcatttta acattcaaat acgcgtaagt
     7201 atatacactt ttttgttgtt taatggtaaa acagcggcaa aacacggtta tttcactaat
     7261 tataaattac acaatataat gtgtaaaatc gtaataactg gcattaggag attcactttt
     7321 tttatcttta caacaaacaa catccatgtc ggagaaactt cagcaggann nnnnnnnnca
     7381 cactattttt tcatcagtgt ggcaatttaa aaaagagtcg ccgcagcgac tcttgtcatt
     7441 aatagcaata attgttttat cttattattg gttgcgcttc tggataatca tcaatatttg
     7501 gatacttaat tgtaagcttt ttattaattt ccatagttga ttcagtaaat gtctttaatt
     7561 cttcgctagc aactaatact tttggttcga cttcttcgtt atatttgtta gtaaccatat
     7621 caatatcagt ccgtattttt tcaac
//
LOCUS       DAOPCD010000024         7229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_9290_length_7229_cov_4.316839, whole genome shotgun sequence.
ACCESSION   DAOPCD010000024 DAOPCD010000000
VERSION     DAOPCD010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7229
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9290_length_7229_cov_4.316839"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1585
                     /locus_tag="PLQ55_01345"
     CDS             245..1585
                     /locus_tag="PLQ55_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPK28617.1"
                     /translation="MNKKEKKLKPGIKKKMSPYLIIVLSFLSVIIIGALLLSLPFAKQ
                     EGVTLSFLDTFFISTSAVTITGLSPIHNLSTTFSVFGKVVIALLTQIGGLGIITLSVF
                     VMILIGMKIGISNRVLLQTNLNSPSMAGVVNLIKKIVIFTLIVEFIGFLINLAVFIPL
                     FPLPKAIGISAFHAIASFNNSGFDILGDSSLINYQDNILLLINTALMIMIGGLGFIAI
                     QDIFTKRSIKKLSIHTKIVIKVNLFLWIVGTLVIKLAQIGGESTSWLTAFFMSVSART
                     AGFAAEKLVNFHSLSLLMLICLMFIGGSPSSTAGGIKTTTFYTLTKSMISYGLGKPTT
                     TYNRVIDQESKNKAFLLLFTALTGVVIGTALILFFDNLTIGRALFETVSALSNVGLSV
                     GTTATLSSASKVVLIVLMFAGRVGPLTIMSLMNRDWYKIGAHPVDYLEEKIIIG"
     gene            1595..2257
                     /locus_tag="PLQ55_01350"
     CDS             1595..2257
                     /locus_tag="PLQ55_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPK28618.1"
                     /translation="MKSIAIIGLSRFGLSLVEAFSKLDVELVAIDMDEEQVRKAGDFI
                     QNVFIADSTSIESLRAAGIANVDHAIVAIGQNERINLTTSIISVIKLKELGIKEITAR
                     ADEEDYAEVLTLVGATNIVMPLDIAAERIANKIAAGNVLDYFNVRRDYDVYEVKISDD
                     FEPLPIINLDSRTRYKINILLIERNKVLYVPTKDTILEPGDEIFVFGKKQDIPKVLSV
                     FN"
     gene            2355..3578
                     /gene="ugpC"
                     /locus_tag="PLQ55_01355"
     CDS             2355..3578
                     /gene="ugpC"
                     /locus_tag="PLQ55_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HPK28619.1"
                     /translation="MAKVKLSGIYKRYEGNKNFSVSDFNLEIGEKEFIVFVGPSGCGK
                     STTLRMIAGLEDITDGELYIDGVLVNDLPPKDRGIAMVFQSYALYPHMTVFDNMAFGL
                     KVQRLPKDEIKRRVHEAARILEIEHYLQRKPRALSGGERQRVALGRAIVRQTEVVLMD
                     EPLSNLDAKLRVQMRSEIIRLHETLGSTTIYVTHDQTEAMTMASRIVVMKDGFIQQVG
                     SPIEIYHNPANLFVASFIGSPPMNFFNVVVQKDHLEFVHYEEREEETEDGEDKVELVS
                     VKERVVDGKLPIPASLAKKLKGYVGKEVVFGVRPENVNYATSEGGREYANFKQNFTVE
                     IAELLGNEYIVHSKLFGQKIISKLLVDPANAIHIGAKLDLVIDFDKVAFFKVDTEAEA
                     AELPSAELNKRIELK"
     gene            3783..5090
                     /locus_tag="PLQ55_01360"
     CDS             3783..5090
                     /locus_tag="PLQ55_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPK28620.1"
                     /translation="MFKQKRIALLVAGLLLLAGCNGGSSADVSGDVSQGTTSQTTTTS
                     GGGSKERVRLSVWGSSAEEPLYHKHAAEFQEKHPEINFSISYADVGEADAATNVLQDV
                     STAADVFFFADDQLPTMQAREVIAKLPKGYANKVKERDVASAAENASSAVDDELYAFP
                     VTNDNGYMLIYNKALLTETDVETLEGIMAKTTAEHQFVVDMGNGYYATSFIQYLCDIT
                     YNPITRLHTTNFNSAEAVDALQGVSNLLKPRVDNGFKSVDFNGAALDDLSDANGNKVI
                     AAVTGVWNTELLKEKLGENLGATKLPTFKSANGTTVQWGSFAGSKLVGVKSSSNNLGW
                     ALAFADYITDEAAQRYRFETNGWGPSNKNLLADEELLADHVTLKALGAQAPYAISQAK
                     SVGSKFWDNAAIVGNFLWKGPASEESPQTLQATLDAFVDALTK"
     gene            5192..6847
                     /locus_tag="PLQ55_01365"
     CDS             5192..6847
                     /locus_tag="PLQ55_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPK28621.1"
                     /translation="METSDKKVKSAHSRTAGTWFKDFLSTIGGFFVRLGKGIVNFFID
                     TGIAIKNTFVNLWKMIKNFFVSVYTHFVKGGWRTKLSHFIMGSGQMSMGRPIKGIIYL
                     LIQVAYFIFMFVPGGGLAWLIKFRTLGEVAVEYMAECTWPPGAPIEDCDPDFITYEKP
                     IFKDNSLLIMLFSVATILITVFFIVMYFVQVRGTFKAEKAYNEAKAKYELAKSEGSEE
                     AFKNPLPKFTDELKSLLNERFHFTTLSLPAITLTMFTILPLVFMILLAFTNYNRDTAP
                     PNKLFTWVGLDTFKNLFSTKGASATNATFGSAMVRILKWTFTWAFFATFTNYIFGLIL
                     AMIINRKSIKLKKLWRTIFVISIAVPQFVTLLLMNQLLKEYGPLNETLLQWGWIRQRI
                     KFLDDPVLAKITVIVVNIWVGVPYTMLLTSGILMNIPTELYESAKIDGAGPVRQFFQI
                     TMPYILFVTGPYLITQFIGNINNFNVIFFLTGGGPSTGIPGVPYGHTDILITWLYALT
                     VGGAHEEYAIGSALGIFIFLISATITLVLYSRTSAAQSEGDFA"
     gene            6849..>7229
                     /locus_tag="PLQ55_01370"
     CDS             6849..>7229
                     /locus_tag="PLQ55_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28622.1"
                     /translation="MSKNVKKDVVTEQPTGLHASAASSRIPKGKVKFWQNQRVIRGVV
                     DAFLYVLLAAMCVAWIFPIIWLIANALRIESTGPVSYLFPREIGFDNFRRLFTTAEMP
                     YLKWFTNTLIVATFSSIISTTIVLL"
BASE COUNT         2262 a         1322 c         1387 g         2258 t
ORIGIN      
        1 aatcgtcgtc ggcatcggtg ttgctgcatt aagtttttca atgtttgata tcggggattt
       61 tagaccactt agtaataaca taataagtgg caaggtcatt aatctttttt tcatcattaa
      121 ctccaatctt cttattaaat taacatttaa attataccat ttatccttaa gtaacaaaga
      181 agggcgatat tttatttagt gttatcttta acactttcat gaataaaagc ataaaatata
      241 aaatatgaat aaaaaagaaa agaaattaaa accaggtatt aaaaagaaaa tgtcgccata
      301 tctaattatt gtgctttctt ttttaagtgt aattattatt ggtgccttgt tactttcatt
      361 accgtttgcg aagcaagaag gagtaacact ctcatttttg gatactttct ttatttctac
      421 ttctgcggtt acaataacag gtttgtcacc cattcataat ttatccacta cattttcagt
      481 atttggtaaa gttgtaattg cactattaac acaaataggt gggcttggca tcatcacttt
      541 aagtgtgttt gtgatgattt taattgggat gaaaattggg atttctaatc gtgttctttt
      601 acaaacaaat cttaactcac caagtatggc gggtgttgtt aatctaatta agaaaattgt
      661 catatttaca ctcattgttg aatttatcgg ttttcttatt aatctagccg tttttatccc
      721 gttatttcct ttgccaaaag cgattggtat aagtgctttt cacgccatag cgtcatttaa
      781 caattcaggt tttgacatat taggggatag ctctttaata aattatcaag ataatattct
      841 cttactaatc aatactgcac tgatgattat gattggtggt cttggcttta ttgctatcca
      901 agatatattt acaaaacgtt caattaagaa attaagcatc catacaaaaa tagtaattaa
      961 agttaacctt ttcttatgga tagttggtac tttagtcatc aaacttgcgc aaattggcgg
     1021 tgaatcgaca agttggctta ctgctttctt tatgagcgtg agcgcgagaa ccgctggttt
     1081 tgctgctgaa aaattagtaa atttccatag tttaagttta ttaatgctta tttgtttaat
     1141 gtttatcggc ggttcaccta gttccactgc tgggggtata aagacaacaa ctttttatac
     1201 tcttacaaaa agtatgatta gctacggact tgggaaacct actacaactt ataaccgtgt
     1261 tattgaccaa gaatcaaaaa ataaggcgtt tttgctgctg tttaccgcat taaccggcgt
     1321 tgtaataggg accgcgctaa tcttattctt tgataattta actattggac gagctctttt
     1381 tgaaactgta tctgcattgt ctaatgtggg cttatcagtc ggaactacgg cgacactaag
     1441 cagcgctagc aaagtcgttt taattgtact tatgtttgca ggaagggttg gtccgctaac
     1501 aattatgtca ctgatgaatc gcgactggta taaaattgga gcgcatccag tagattattt
     1561 agaagaaaaa ataattatag gttaaaatat atttatgaaa agtattgcaa ttattggttt
     1621 aagtagattt ggcttaagtt tagtagaagc ttttagcaag ttagatgttg aattagtcgc
     1681 tattgatatg gacgaagagc aagtccgtaa ggccggcgac tttattcaaa atgtttttat
     1741 tgctgattcc actagtattg agtctttaag agctgctggt atcgctaatg ttgatcatgc
     1801 aattgtcgcg ataggacaaa atgaaagaat caacttaact actagcatta tttcagtcat
     1861 caaattaaaa gaattaggca ttaaagaaat taccgctcgt gctgacgaag aagattacgc
     1921 agaggtttta acattagttg gtgcgactaa tatcgttatg ccacttgata tcgcagcgga
     1981 aagaatcgct aataaaatag cagccggtaa tgttcttgat tatttcaatg tccggcgcga
     2041 ttacgatgtt tacgaagtta aaataagtga tgactttgaa ccacttccaa ttatcaacct
     2101 tgactcacgt acccgctata aaataaatat attgttaatc gagcgcaata aagtgctcta
     2161 tgttccaaca aaagatacga tattagaacc aggtgacgaa atttttgttt ttggaaaaaa
     2221 acaagacatt ccaaaggttc ttagtgtctt taattaatct tcttcttggt tatgaaaatt
     2281 ttaaatataa catcgattgg cacaaattta aaaatgtgtg ctatgattaa attaattgaa
     2341 gaaggagttt tattatggcc aaagtaaaat tatcaggcat ttacaaacgt tacgaaggta
     2401 acaaaaactt ttcggttagt gattttaacc tagaaatcgg tgaaaaagaa tttattgttt
     2461 ttgttggtcc atcaggctgt ggtaagtcca caactttacg gatgattgct gggttagaag
     2521 atattaccga tggggaactt tacattgacg gagtgctcgt taatgactta cccccgaaag
     2581 accgcggaat tgcaatggtt ttccaaagct atgcacttta tccgcatatg actgtgtttg
     2641 acaacatggc atttggacta aaagtccaac gtctcccaaa agatgaaatc aaacgccgtg
     2701 ttcatgaagc agcaaggatt cttgaaatcg aacattatct ccaaagaaaa ccacgtgcgc
     2761 tctctggtgg tgaaagacaa cgggttgctt taggacgtgc aattgttcgt caaactgaag
     2821 ttgtattaat ggacgaacct ttatcaaact tagacgcgaa acttcgtgtg caaatgcgtt
     2881 cagaaattat tagacttcac gaaacattag gtagtacaac aatttatgtt actcacgacc
     2941 aaaccgaagc aatgacaatg gcctcacgta tcgttgttat gaaagacgga ttcatccaac
     3001 aagttggttc accaattgaa atttatcata atccagcgaa cttattcgtc gctagtttca
     3061 ttggttcccc accgatgaac ttctttaatg ttgttgtgca aaaagaccat cttgaattcg
     3121 ttcattatga agaacgcgaa gaagaaactg aagatggcga agataaagtc gaactcgtca
     3181 gtgttaaaga acgtgtcgtt gatggtaaat taccaattcc tgctagttta gcgaaaaaac
     3241 ttaagggtta tgttgggaag gaagtcgtct ttggcgtccg tccagaaaat gtcaactatg
     3301 ccacaagcga aggtggccgc gaatacgcta actttaaaca aaacttcact gttgaaatcg
     3361 ctgaattact tggtaacgaa tatatcgttc actccaaatt attcggtcaa aaaattattt
     3421 cgaaattact cgtcgatccc gctaatgcaa tccacattgg ggcaaaactt gacttagtaa
     3481 ttgactttga caaagtcgct ttcttcaaag ttgataccga agcagaagct gccgaattac
     3541 cgagcgctga attaaataaa cgtatcgaac tcaagtaata atcaacaaaa ttcgagaaaa
     3601 agctccgcta acaggagctt tttttgatta agatcaaaag gtttccaaat tatatacata
     3661 tatcatttgt caaaatataa aataattact ataatgacta tgtctaaact taaatattgg
     3721 cagagcttaa ctatcgttag catttttagg ctctcccaca aaacaatgag aaaggagaat
     3781 ttatgtttaa acaaaaacgt atcgctttac ttgttgcagg tttgctttta cttgccggct
     3841 gtaatggtgg cagtagcgct gatgtcagtg gtgatgttag tcaaggaaca acaagccaaa
     3901 caacaacaac gagtggaggc ggatcaaagg agcgtgtccg tctatcagtg tggggctcaa
     3961 gtgctgaaga accactctat cacaaacacg ctgctgaatt ccaagagaag catccagaaa
     4021 ttaacttctc aattagttac gctgacgttg gagaagctga tgccgcaaca aacgtccttc
     4081 aagacgtaag tactgctgct gacgtatttt tcttcgcaga tgaccaactt ccaacaatgc
     4141 aagctcgtga agttatcgca aaattaccaa aaggttatgc gaataaagtt aaagagcgtg
     4201 acgttgctag tgctgccgaa aacgcaagtt cagcagtgga cgatgaactt tatgcattcc
     4261 ctgttacaaa tgacaacggc tatatgctta tttacaataa ggcactctta acagaaaccg
     4321 acgtcgaaac tttagaagga attatggcga aaactactgc cgaacatcaa tttgtcgttg
     4381 atatgggtaa tgggtattat gcaacttcct tcattcaata tctctgtgat atcacttata
     4441 atccaattac aagacttcat accactaact tcaacagtgc tgaagcagtt gacgccttac
     4501 aaggtgttag caatctctta aaaccaagag tagacaacgg atttaaatca gttgacttta
     4561 acggtgctgc acttgacgac ttaagtgatg ccaatggtaa caaagttatc gctgctgtta
     4621 ctggtgtgtg gaataccgaa ctcttaaaag aaaaattagg tgaaaacctt ggtgccacta
     4681 agctcccaac atttaagagt gcaaacggaa caaccgtaca atggggttcc tttgctggtt
     4741 ccaaattagt tggtgttaaa tcttctagta ataacctcgg atgggcactt gcctttgccg
     4801 actacattac tgacgaagcc gcccaacgct atcgttttga aacaaacgga tggggtccat
     4861 caaacaaaaa cttacttgcg gacgaagaac tcttagccga ccatgttacc cttaaagcac
     4921 ttggtgccca agcgccatat gctatttcac aagcaaaatc agttggctcc aaattctggg
     4981 acaatgctgc tatcgttggt aacttcttat ggaagggtcc agcaagtgaa gaatcaccac
     5041 aaacacttca agcaacactt gatgcctttg tggatgcctt aacaaaataa tataaaatta
     5101 ttaaacacat ataatcgatt aatttttaat taattttggg agccagttca tcgctggctc
     5161 tcctatcttt ctagaaatga ggccataaaa tatggaaaca agtgataaaa aagtcaaatc
     5221 ggcccactca cgtacggctg gcacttggtt taaagatttc ttgtccacaa ttggtggttt
     5281 ctttgtgcgt ttaggcaaag gaatcgtcaa tttttttatt gatacaggaa ttgccattaa
     5341 aaacactttc gttaatttat ggaaaatgat taaaaacttt tttgtatcag tttacacaca
     5401 ctttgtcaaa ggaggatggc gcactaaact ctcgcacttc ataatgggaa gtggacaaat
     5461 gtcaatgggc cgtccaataa aaggcattat ttatctttta atccaagtag cgtatttcat
     5521 tttcatgttc gtcccaggag gaggactagc atggttaatt aaatttagaa cactcggaga
     5581 agttgcggtt gaatatatgg cggaatgtac ttggccacca ggtgctccta ttgaagattg
     5641 tgacccagat tttattacat atgagaaacc tatctttaaa gataactcgc tcttaattat
     5701 gttattctcg gtcgcaacaa ttttaattac cgtctttttc atcgttatgt actttgttca
     5761 agttcgcggc acttttaaag cagaaaaagc ttataacgaa gcaaaagcaa aatatgaact
     5821 tgcgaaaagt gaaggtagtg aagaagcatt taagaaccca cttccaaaat ttactgatga
     5881 acttaagtca ttattaaatg aaaggttcca cttcacgacg ctctcacttc cggcaataac
     5941 attaacgatg tttacgatat taccgcttgt ctttatgatt ttgctcgcgt ttactaacta
     6001 taaccgcgac actgccccgc cgaataaatt atttacttgg gttgggttag acacatttaa
     6061 aaatttattt agtacaaaag gcgcttccgc tactaatgcg acatttggta gtgcgatggt
     6121 aagaatcttg aaatggacat ttacctgggc attctttgcg acttttacta actatatttt
     6181 cggattaatt ctcgcaatga ttattaatcg caagagcatt aaattaaaga aactgtggcg
     6241 aaccatcttt gttatttcca ttgccgttcc ccaatttgtt accttgctct taatgaacca
     6301 attacttaaa gaatacggac cgcttaatga aacactatta cagtggggat ggattaggca
     6361 acggattaaa ttcctcgatg atccagtcct agctaaaatt acggtcattg tcgtaaatat
     6421 ttgggttggg gttccttaca caatgctatt aacgtcgggt atcttaatga atattccgac
     6481 tgaattatat gaatcagcga aaattgacgg cgctggacct gtccgacaat tcttccaaat
     6541 tacgatgcct tacatcttgt ttgttacagg tccgtactta attacccaat tcattgggaa
     6601 cattaataac tttaacgtta tattcttcct gacaggtggg ggaccaagta ctggtattcc
     6661 tggcgtaccg tatggtcaca ctgatatttt aattacgtgg ctatacgcgc ttaccgttgg
     6721 aggagcacat gaagagtacg cgattggtag cgcactaggg atcttcatct tccttatcag
     6781 tgcgactatt actttagtgc tctattcgcg gacatccgca gcacaatcgg aaggagactt
     6841 tgcataatat gagtaaaaat gttaaaaaag atgttgttac tgaacaacca acaggcttac
     6901 acgctagtgc ggcctcctca cgaattccaa agggtaaagt taagttttgg caaaatcaac
     6961 gtgtgatacg cggtgttgtc gatgctttcc tttatgtctt actagcggca atgtgtgtgg
     7021 cgtggatttt cccgattatt tggttaatcg ctaacgcact ccgcattgag tcaactggac
     7081 ctgtttctta ccttttcccg cgagaaatcg gctttgataa ctttagacga cttttcacta
     7141 cagcagaaat gccgtactta aaatggttta caaatacgct aattgtggcg acatttagta
     7201 gtatcatttc cacaactatc gtcttactt
//
LOCUS       DAOPCD010000025         7197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_9324_length_7197_cov_4.166760, whole genome shotgun sequence.
ACCESSION   DAOPCD010000025 DAOPCD010000000
VERSION     DAOPCD010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9324_length_7197_cov_4.166760"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1983)
                     /locus_tag="PLQ55_01375"
     CDS             complement(<1..1983)
                     /locus_tag="PLQ55_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HPK28623.1"
                     /translation="MSANIKTFGLGVFNKSPNVEYKVYENGLYLGNSTLSEHLLLVEI
                     IDKEQTTFKLHPDTRTIAEFACYKSNMTEVTSWGNNLKTIGYGAFMQSKLTAITLPNS
                     LETIADSAFKQTKLTSLNFGTGLKAIGYEAFSNCPDLADVTLPNSLERIDSSVFYGTS
                     VTYTDYENGRYLGSGDAGETANPYFALMEVITKTMTTFTVHADTHLIVPGVFYGSSSL
                     ESVEIKEKVSFIGSGAFENCIALTSLSVAANNTHFYSPSNKAILEKDTDRLLFGLNGG
                     FIPEDTKIIGEKAFYYRKFTEPLVIPSSVTTIEDYAFYQTNLTAIVIPDSVTKIGKSV
                     FQYSNSLTSVTLSKNLKVIEDWTFSGCKILETLTIPDSVTMIKEWAFSNCEMLANVTY
                     SANLAFIGDVAFSSCVALTTLPASESLRVFGSGSFRGCESLTSITIPKNVRYISSGAF
                     ADCTALTTLNVVSENNHYDSRENCNGIIETATNTLMVAVKDTVIVESIEALGAEVFSG
                     IDIQSITIPASITKIGNWAFSDCGKLETVVIEGELTMINAGTFSHCTSLTSVNIPETV
                     TEIGGSAFYNCKVLPEIKLPANLEKIGSWCFGRCTILPQIVIPRSVTNIARSAFSSCA
                     NLTIYAEATALPDGWHADWNDGNRPVYWYSETANYDG"
     gene            complement(2026..2502)
                     /locus_tag="PLQ55_01380"
     CDS             complement(2026..2502)
                     /locus_tag="PLQ55_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HPK28624.1"
                     /translation="MPKYDGATPTRPDDDTYTYSFNGWDPEPVPVVADATYVAKYKTA
                     LKEVVDPRDFVFADTGEGYTLCGYYGSASRVVIPETYNDKPVVSLEKWAFFNLDNLVS
                     VTLPNNLRKIGEDVFADCDNLKDVDFGNGVQEIGFMAFDHCPSLEAISLPDSVETI"
     gene            complement(2879..3994)
                     /gene="ychF"
                     /locus_tag="PLQ55_01385"
     CDS             complement(2879..3994)
                     /gene="ychF"
                     /locus_tag="PLQ55_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPK28625.1"
                     /translation="MGLSVGIIGLPNVGKSTLFNALTASKVEAANYPFATINPNKGTV
                     RVLDPRVTKLAEYYEPEKTIYATIEFNDIAGLVRGANRGEGLGNQFLGHIRECDVILE
                     VVRCFSGEEIIHVEQSVDAKRDIEIINLELILKDLEVLYRRKDKVETKARVNKDKDSV
                     FELEIINKLIPALENEVPARLVTTLTQAETDYVKLHFGLLTIKPLIYVANISDTEYAT
                     YEDNVEYQKVKAIAKREGAELVAISAEIEAEIATLSSADQELYLAELGINESGLNKVI
                     NVAYKTLGLSSFFTTGKDEVRAWTFKDGTSAQKCAGIIHSDFERGFIRAEVYSYNDFE
                     KYGSEQKIKEAGRVRSEGKDYVMRDGDIVFFKFNVRK"
     gene            complement(4098..4295)
                     /locus_tag="PLQ55_01390"
     CDS             complement(4098..4295)
                     /locus_tag="PLQ55_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPK28626.1"
                     /translation="MKGTIKQFNQERGYGFIRAEDGTDVFFHYSSLVMDGFKTITAGE
                     KVEFTLEQSDRGPRASNIVKI"
     gene            complement(4410..7112)
                     /locus_tag="PLQ55_01395"
     CDS             complement(4410..7112)
                     /locus_tag="PLQ55_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK28627.1"
                     /translation="MISIKNLECVLGGERILNIASFEINAPGLYVILGPSGCGKTTLL
                     NIITGLNSEYTGSVELFNRTYRHASKKETTTFRSRYLSAFFQHSVFIEELTMRQNISL
                     VALNHTKSLENLVNSKVEKLAKRLNIEKVLNQKVKTLSGGEKTRGAIARTLLKEVPLY
                     IFDEPTAALDRENAVIVMDLIREYSKTNIIILVTHDESLANEYADTIFTMEYGEFKSV
                     NIIRNNELRVAKALPEIPYAKNSDYIARGLLKAKKARHFFTGGAVNLGLVGLGLSFML
                     VNAINYKLVNTFKGEFTNETVYVQKKFAPEINVLKSVSHEEIALNISAEKELGSFYLN
                     NFNDFFPHQNELSFNHLGIRHVLPSFHIGLLNESLYINEIHEQLYPYVTNLAIDEIGL
                     VLPFDDFKLIQNVLRIPMRQTPSELGVYLENNNFYVTLEVTNNNWDYFDEQIFRLRTV
                     TLGREAQIIMGEPSFIPFIFEEKMMFPSSLNLTKIEEYPWTLKKINYMLLNNAFDYLW
                     QSLNYSNYVFCTANNDYFQYIYNKTYLQNRLLIFESPPHFNSMFKSYSSESEAEFYTF
                     SNGLTFIPEMMMLGFAHNFFITQDITLLEAAMWADINDHSAPSSYLELPEGILNFAIQ
                     YNGFSSFNYRNANTPLRIDELIISSGLANKLGLSKDAHNTELHIGALVAIAEASEGYL
                     KEYETIKVSIKRVIDNDEVVIYHHPLWAYLLYKDVFNVFPFALEITGAIYQSVTNEDL
                     KNLQKHFSVSYPYKDFSVLVADTLNELERYTFLASVGAFLLAALITFMTIYLLVAETS
                     DQFSGLFLVGYNEEAIHGVISRYILRFLGVIILLAIVQLFILSFVVEVVLMKYLETSF
                     RYVFNPKPYLLVLAFATGLLIFLLTYFKFQHEKLDFLAFSKRDL"
BASE COUNT         2334 a         1414 c         1091 g         2358 t
ORIGIN      
        1 accatcgtaa ttagcggttt cactatacca ataaacagga cgattaccat cattccaatc
       61 agcgtgccat ccatctggta gtgctgttgc ttcggcataa attgttagat tcgcacaact
      121 gctaaaggca gaacgagcaa tatttgttac acttctagga atgacaattt gcggtaaaat
      181 ggtacaacga ccaaaacacc aactaccaat tttctccaaa ttagcgggca gtttaatctc
      241 tggtaaaact ttacaattat aaaaggcact gccaccaatt tcagtcactg tttccggaat
      301 attaacactc gttaaggaag tacaatgtga aaatgtacca gcatttatca tcgtaagttc
      361 accttcaatt acgactgttt ctagctttcc acagtcacta aatgcccaat taccgatttt
      421 agtgatactt gcgggaatcg taatactttg aatatcaatg cctgaaaata cttctgctcc
      481 taaagcttca atactttcga caatcaccgt atctttaacg gcgaccatta atgtatttgt
      541 ggccgtttcg ataataccat tacaattttc gcgtgagtcg taatgattat tttcacttac
      601 aacatttaat gttgttagcg ccgtacaatc agcaaaagcg ccgctagaaa tatagcgtac
      661 atttttagga atcgtaatac ttgttaaact ctcacaccct ctaaatgacc cactgccaaa
      721 cactcgcaaa ctttcagatg ctggaagggt agttaaggca acgcaacttg aaaaagcaac
      781 gtcaccaata aacgctaaat tagcgctgta agtaacatta gcaagcattt cacaattact
      841 aaatgcccac tcttttatca ttgtgactga gtcgggtatc gtaagtgttt ctagtatttt
      901 acaaccacta aaagtccagt cctcaatgac ttttaagttc ttacttaagg ttactgaagt
      961 taggctatta ctatattgga acactgattt tccgatttta gtgacactat caggaatgac
     1021 aatcgctgtt aaattagttt gataaaaagc ataatcttca attgtcgtga cactacttgg
     1081 aatgactaaa ggctcagtaa atttccggta ataaaatgct ttttcaccaa tgatttttgt
     1141 gtcttctgga ataaaaccac catttaggcc gaatagtaaa cgatcggtgt ccttttctaa
     1201 aatcgcttta ttcgatggcg agtaaaaatg cgtgttatta gcggcaacac taagagaggt
     1261 taacgcaata caattctcaa atgctccgct accaataaat gacaccttct ctttaatttc
     1321 cacactttca agactggaag atccataaaa aacaccaggc actataagat gagtatcagc
     1381 gtgaacagta aatgtcgtca tcgtttttgt tattacttcc attaaagcaa aatacggatt
     1441 agcggtttct ccggcgtctc cacttcctaa atatcgtccg ttttcgtaat cagtgtaggt
     1501 aacgctagtg ccataaaaaa cacttgaatc tattcgttct aaactattag gcaatgtaac
     1561 atccgctaaa tcgggacaat tactaaacgc ctcataacca attgctttaa gtcctgtccc
     1621 aaaatttaga cttgttagtt tcgtttgttt aaaagcagag tctgcaattg tttctaaaga
     1681 atttggtaat gtgatggctg ttaatttaga ctgcataaag gcaccataac ctattgtctt
     1741 aagattatta ccccatgaag ttacttctgt catattactt ttgtaacagg caaactccgc
     1801 aatagtccgc gtatcaggat gtaatttaaa tgttgtttgt tctttgtcaa tgatttctac
     1861 aagaagtaaa tgttcgctaa gtgtactatt cccaagatat aagccatttt cataaacttt
     1921 atattcaaca ttcggagact tattaaaaac gcctaaccca aaagttttaa tattcgctga
     1981 catccgaaca cttgttaatg acggacaatt agcgaatgct tcaccctaaa ttgtctcaac
     2041 actatctggt aaagaaattg cttctaaact tggacaatga tcaaatgcca taaagccaat
     2101 ttcttgtacg ccattaccaa aatcaacatc ttttaaatta tcacaatcgg caaacacatc
     2161 ttcaccgatc ttccgtaaat tatttggaag tgtcactgac actaaattat caagattgaa
     2221 aaatgcccat ttttcgagcg ataccactgg tttatcattg taagtttcag gaatgactac
     2281 cctactagca cttccgtaat atccgcataa agtgtaacct tctcctgtat cagcaaagac
     2341 gaaatcacgc ggatccacaa cttcttttaa agctgtttta tattttgcta catatgttgc
     2401 atcagcaaca acaggtacag gttcaggatc ccagccatta aacgaatatg tatatgtatc
     2461 atcatcaggc cgtgttggtg tagcgccatc atacttcggc atcgtgccgc gctccacatt
     2521 tttatcaact tccagtattt ggctattata atttttccat gtaattgtaa aagttgttgc
     2581 ttcgcttcct gagtcacctg cacttgtgat agtttcaggc tccgttgtat cagtgcttgt
     2641 tgtaacactt ggactctcgc tacttggttt tttaccacat gatgttaaag ctaaaaacgc
     2701 taaaataagc caaaaagcta ttgtaaattt ttcataaaaa tcttccccct acgataactt
     2761 cattttactt ttaaaagaaa gtaaagcaaa gcttttttta ctaccttatt aacttttaca
     2821 atttataaaa ataattagtt atttataatc aatattattt aaaaattcaa ttttttattt
     2881 attttcgcac attaaatttg aaaaagacaa tgtcgccatc acgcattaca tagtctttgc
     2941 cttcactgcg gactctacca gcttctttaa ttttctgttc actaccatac ttttcaaaat
     3001 cattatatga ataaacttcc gcacggataa agccgcgttc aaagtcactg tgaataatac
     3061 cggcacactt ttgcgcacta gtgccatctt taaatgtcca cgctctaact tcatctttgc
     3121 cggtcgtgaa aaaagaagaa agtcctaatg ttttatatgc aacattaatt actttattta
     3181 accctgattc attaatccca agttctgcta aatataattc ttgatcagca cttgataaag
     3241 tcgcaatttc tgcttcaatt tcagcactga ttgccacaag ctctgcgcct tcacgcttag
     3301 caattgcttt tactttttga tactcaacat tatcctcata agttgcgtat tccgtatcac
     3361 taatattagc gacataaata agcggtttaa tcgttaataa accaaagtgg agcttaacat
     3421 aatctgtttc tgcttgcgtt aaagtagtaa ctagtcgcgc tggcacttcg ttttctaagg
     3481 cggggataag tttattaata atctccagtt caaacactga atctttatct ttatttaccc
     3541 gcgcttttgt ttcaacttta tctttcctac gatataaaac ttctaagtct ttaagaatta
     3601 attctaaatt aataatctca atatcgcgtt tagcgtcaac actttgttcg acatgaatta
     3661 tttcttcgcc actaaagcag cgcaccacct ctaaaataac atcacattcc ctaatatgtc
     3721 caagaaattg gttaccaaga ccttcaccac gattagcgcc ccgcactaaa ccagcaatat
     3781 cgttaaattc aatcgttgca tatattgttt tttcgggttc ataatactcc gctagttttg
     3841 tgacacgcgg gtcaagtaca cggacagtac ctttatttgg attaatcgtc gcaaaaggat
     3901 aattagcggc ctcaacttta ctagcggtta aagcattaaa taatgtcgat ttgccgacat
     3961 taggtaagcc aataattcca acacttaatc ccataattta ctcccgcagt attatattac
     4021 tattagtcac gataagcaat agtgacaata aaaggctgct catgagaaca gcctcttatt
     4081 tactcattta agttaaatta tatcttaaca atattagatg cgcgtggtcc gcgatctgat
     4141 tgttccaagg taaattcgac tttttcacca gcagtaatgg ttttgaagcc atccattaca
     4201 agtgatgagt agtggaaaaa gacatcagtg ccgtcttctg cgcgaataaa gccgtaacct
     4261 ctttcctgat tgaattgttt aatcgtccct ttcatcagta atccttccat gacatatcgt
     4321 ctatttgtgt ctaagcacaa ttgtattata acaaaaagaa aatttttcgt tgcttaaaat
     4381 aggaaataaa aagtaagaaa gtcgtaaagt tataaatcac gtttcgaaaa cgctaaaaaa
     4441 tcgagttttt catgctgaaa tttaaaataa gttagtaaga aaattagtaa tcctgttgca
     4501 aacgctaaga caagtaaata tggtttcggg ttaaacacat atctgaaact agtttctaaa
     4561 tatttcatca acactacttc aacgacaaaa gataaaataa ataactgcac aattgctaaa
     4621 agaataataa cacctaggaa gcgcaggata tatctactaa tcaccccatg aattgcttct
     4681 tcgttataac ccactaaaaa gagcccgcta aattggtcgc ttgtttcagc aactaataag
     4741 taaattgtca taaaagtaat aagggccgct aaaagaaaag cgccaactga tgctaaaaaa
     4801 gtataacgct ctaactcgtt taatgtatcg gcaacaagca ctgaaaaatc tttataggga
     4861 taagagacac tgaaatgttt ttgtaaattt ttcaagtctt cattagtgac actctgataa
     4921 atagcgcccg ttatctctag agcaaacggg aaaacattga atacatcttt ataaagtaaa
     4981 tatgcccata atgggtgatg gtatattaca acttcatcat tatcgattac ccttttaatc
     5041 gacactttta ttgtctcgta ttcttttaaa taaccttcac ttgcttctgc aatcgctaca
     5101 agtgcgccaa tatgtaattc cgtattatgt gcgtccttgc ttaaccctaa tttatttgct
     5161 aaaccagaag aaataattag ttcatcaatt cgtaatggtg tattagcgtt ccggtaatta
     5221 aaactagaaa acccattata ttgaatggca aaatttaaaa taccctcagg taattctaag
     5281 tatgaactag gtgcactatg atcattaata tctgcccaca ttgccgcttc taataaagta
     5341 atatcctgcg taataaaaaa gttatgagca aagccaagca tcatcatttc tggaataaaa
     5401 gttaaaccat tactaaatgt ataaaattca gcttcactct ctgaagaata acttttgaac
     5461 atactattaa aatgcggagg actttcaaaa ataagtaacc tattttgtaa ataagttttg
     5521 ttgtaaatat attgaaaata gtcgttattt gccgtgcaaa acacataatt tgagtaattt
     5581 aaggattgcc acaagtaatc aaaagcatta ttaagtaaca tataatttat ctttttaagt
     5641 gtccacggat attcttcgat tttcgttaaa tttaatgaac ttgggaacat cattttttct
     5701 tcaaaaataa atggaataaa actaggctca cccataataa tttgtgcttc tctgccaagt
     5761 gtaactgttc gtaatctaaa tatttgttca tcaaaataat cccagttatt attagtcact
     5821 tcaagcgtta cataaaagtt attattctct agatacactc ctaattcact tggggtttga
     5881 cgcatcggta ttcgtaatac attttggatt aatttaaagt catcaaatgg taacactaat
     5941 ccaatttcat caattgctaa atttgttaca tatggataga gttgctcatg tatctcgttt
     6001 atatacaaac tttcatttaa taagccaata tgaaaagaag gaaggacatg gcgaatgcct
     6061 aggtgattaa acgacaactc attttgatgt gggaaaaaat cattaaaatt atttagataa
     6121 aaagagccta attctttttc cgcggagatg tttaacgcta tttcttcgtg tgacacactt
     6181 ttaagcacat taatttctgg tgcgaacttc ttttgtacat acaccgtttc atttgtaaat
     6241 tcccctttaa aagtatttac tagtttataa ttaatcgcat ttactaacat aaatgataag
     6301 ccgagtccca ctaatcctaa gttaacagca ccaccagtaa aaaagtgtcg tgctttcttc
     6361 gcttttaata atcccctagc tatgtaatca ctatttttag catatggtat ttccggcaat
     6421 gcttttgcga ctctaagttc attatttctt attatattta cacttttaaa ttcaccatac
     6481 tccattgtga aaatggtatc agcgtactca ttagctaaac tttcatcatg cgttactaaa
     6541 atgatgatgt ttgtttttga gtattcccta attaaatcca ttactattac agcattttct
     6601 cgatcaagcg cagcggttgg ttcatcaaaa atataaagcg ggacttcttt taagagtgta
     6661 cgagcaatgg cgcccctagt tttttcacca cctgacagtg tttttacttt ttgattaagt
     6721 actttttcaa tatttaaacg cttcgctaat ttttcgactt tagagtttac taaattctcc
     6781 agcgacttag tgtgattaag cgctacaaga ctaatgtttt gtcgcattgt taattcttcg
     6841 ataaaaacac tgtgctgaaa aaaagcgctt aaatagcgcg acctaaaagt tgtcgtttcc
     6901 tttttactag cgtgacgata agtgcggtta aagagctcaa cacttccagt atattcacta
     6961 tttaaaccgg taataatatt taaaagcgtt gtttttccgc aacctgatgg accaagaatc
     7021 acatataacc ccggagcatt aatctcaaaa ctagcgatat taagaatccg ctctccaccg
     7081 agtacacatt ctaaattttt aatactaatc atctattaat aaatacaata gtgggcgacg
     7141 catatttatc aagaaaataa ttgtgattaa aataagtagt gttagaagat cagcgct
//
LOCUS       DAOPCD010000026         7173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_9366_length_7173_cov_3.499719, whole genome shotgun sequence.
ACCESSION   DAOPCD010000026 DAOPCD010000000
VERSION     DAOPCD010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9366_length_7173_cov_3.499719"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PLQ55_01400"
     CDS             complement(<1..363)
                     /locus_tag="PLQ55_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002833327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPK28628.1"
                     /translation="MADLTKFDLQHTRNIGIMAHIDAGKTTTTERILYYTGRLHKVGE
                     THEGASAMDWMAQEKERGITITSAATTAFWKENRINIIDTPGHVDFTVEVERSLRVLD
                     GAVTVLDGKSGVEPQTETV"
     gene            complement(372..842)
                     /gene="rpsG"
                     /locus_tag="PLQ55_01405"
     CDS             complement(372..842)
                     /gene="rpsG"
                     /locus_tag="PLQ55_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPK28629.1"
                     /translation="MPRKGSVAKRDVLPDPIYHSKLVTRLINKLMYDGKRGTAQTILY
                     NAFERIEAKTKQPAMDVFATAISNIMPEVELKVRRVGGQNYQVPTDVSPERKTTLGLR
                     WLVNYSRLRNERTMEERLAAEIIDAANGTGASVKRRDDTHKMAEANKAYAHYRY"
     gene            complement(890..1312)
                     /gene="rpsL"
                     /locus_tag="PLQ55_01410"
     CDS             complement(890..1312)
                     /gene="rpsL"
                     /locus_tag="PLQ55_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018395150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPK28630.1"
                     /translation="MPTINQLVRHGRQKAVYKSKSPALTKRWNSLKRREVNQYSSHKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNGYEVTAYIGGEGHNLQEHSTVLIRGGRVKD
                     LPGVRYHIVRGTLDTAGVEKRRQGRSQYGTKRPHTAEH"
     gene            complement(1568..1741)
                     /locus_tag="PLQ55_01415"
     CDS             complement(1568..1741)
                     /locus_tag="PLQ55_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28631.1"
                     /translation="MDGNKRIGLYVMLSFLQINGIIISFTDDEIEQIAFGIASGAINY
                     VDLKRILEQKIDN"
     gene            complement(1754..1873)
                     /locus_tag="PLQ55_01420"
                     /pseudo
     CDS             complement(1754..1873)
                     /locus_tag="PLQ55_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(1944..2180)
                     /locus_tag="PLQ55_01425"
     CDS             complement(1944..2180)
                     /locus_tag="PLQ55_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HPK28632.1"
                     /translation="MIIKTEQIITMTDANQNFSKASKTAEKYGSAVIFKNNKPKYILA
                     DIEQYIELTEDEKIEIVAKRILSKHINAFKNLAK"
     gene            complement(2302..3678)
                     /locus_tag="PLQ55_01430"
     CDS             complement(2302..3678)
                     /locus_tag="PLQ55_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPK28633.1"
                     /translation="MHKRKPKKITTILFSLFLALSLSVVTGCKDGSENGGSDDPINFW
                     AYQPSKQTDQTAYKNLIADFTAETGIKVKLLMVVKDSYNKSLNAAISSRSKPDLAYFD
                     QPRISDYAHDDTIAKLDEYIANSAVIDNTKFFASGLATTVYKGSQYGVPLNMTTSVLF
                     YNKDIVTTPPTSWQEWLNTPVTGHNQSLFDGIGTGGYAGWFTDPELTIAYVPGLLTAD
                     LTLYGKYVESQDTTLIFYDPNKVLSSGDVYLYTWDGDGRKITGNWPGTAMFNHGFGYY
                     SWFFHAAYLTENVIFHDNAGNQTGDIVWEIGKNVYHQETGTWTFVSEVHHKATVYASK
                     ILEKTATCDATGAANNLNAPEWNETLANQYNDSLDPTTKAYLKTAVADSNGDLLAQAM
                     ARYDYIVKKYGTAMFSNFLDRTIPASGSPLVSNEGTNNALVMGAFIVMALVALAGFKL
                     ISKKHRYH"
     gene            complement(3756..4709)
                     /locus_tag="PLQ55_01435"
     CDS             complement(3756..4709)
                     /locus_tag="PLQ55_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK28634.1"
                     /translation="MATLKDIAKATGFSVITVSRVINSPHLVKEKTRKIIEAEIARQN
                     FKPNVLARALVQNRTNIIYVYIPADIDAINPFFLHVVSGIGEVLGEKGFSLLLKRSWY
                     EGEDCDGCIFMGLSAEDEAKVFELSKTKPVCVFGYVEGVSSVDVDNYQGLYLMTSHVL
                     SKGYRTLTYLGIDDSRRFILQRKKGFSDAVMSQKDKITVKYLAAKNNENDAYKVVGQS
                     LKQGDTPEVIVCATDDMAIGAIRAIKEKGLEVPRDIAVTGFDGLGTQEVAHPRLTTIH
                     QPIYEAGKALAHRIIALIENKNELPVNHAFIETTIIHRESI"
     gene            complement(4764..6386)
                     /locus_tag="PLQ55_01440"
     CDS             complement(4764..6386)
                     /locus_tag="PLQ55_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28635.1"
                     /translation="MRKSTFIRRALILVVPALLLTSCVTFGDILKSSEFGATSSSANE
                     VTSEVTGSENPYPLNGTYGVNDYEHPITANPDTLNNYYKVEPLNNNSRLINNYAISNT
                     MGLAHLDSVSNQKMMVVPVKFTDTPASYGGEATIQGAINRAFFGESSDTGWESVASYY
                     YKSSYGRLSLTGEVMPLFDMGYSTTELLAKTVDGYQYWDKSHYVLEQVYNTYLSSTLK
                     EYDQNNDGFVDSVFFVYLAPVDYESDDTLFWAFQYFWNRFADKNKPVFNAYGWASYEF
                     MVEAPGYTYLKPAAHTYIHESGHILSFDDYYDYDGTIAPAGAIDMMDHNIIDHNMFSK
                     FSANWSTPYVPVGNSDIRLRPAESSGDFIIINDAWNGHAYDEYILIEYYTPTGLNARD
                     SLSVSGYNGVKGYTIPGVRIWHVDARLVILSNNGNTKKWSDTIPPHLDGSGGSWMDFA
                     TSNTPSYNYAHNSLHKLHLLDGRGRAQTWANNLVKADDSALFQTGTVIASNNWQKYLL
                     KRATFNDGSVIGYSIEIGEMNSEGVTIKIRKA"
     gene            complement(6388..>7173)
                     /locus_tag="PLQ55_01445"
     CDS             complement(6388..>7173)
                     /locus_tag="PLQ55_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28636.1"
                     /translation="SWSSYYYLDVARNKKPDPHTYIHEVGHLFGLPDYYNTNYDDPYS
                     PLGGFDMMDYTLGDHTGLSKMLLDWARPYALKRSTTIKLRPFSETGDLLVLKNNWNEK
                     ANDEYILLEYYTPTVLNELDSTLNASFKLPKTNGVKVYHVDARTVYETKNGSIVKYPY
                     VNDYEGEFNGTELLAHSNSTGSYNNAPIKTNKLYKLLEPKESTNISESFNQANQHSLF
                     QKGDEFGYEYFKNFAFNDKTPLNFRFKIVQLTNAYATIEIEVY"
BASE COUNT         2084 a         1457 c         1234 g         2398 t
ORIGIN      
        1 cacagtttcc gtttgtggtt caacaccact tttaccatct aatactgtga cagcaccatc
       61 aagtacacgt aatgaacgtt caacttcaac tgtaaagtcg acgtgtcctg gggtatcaat
      121 gatattaatg cggttttctt tccaaaaggc ggttgtcgcc gcacttgtaa ttgtaatacc
      181 gcgttctttt tcttgggcca tccagtccat tgcggatgca ccttcgtgtg tttcaccgac
      241 tttatgtaaa cgaccagtgt agtaaagaat acgctctgtt gtcgttgttt tccccgcgtc
      301 aatatgcgcc ataattccga tattacgcgt atgttgcaaa tcaaatttag ttaaatcggc
      361 catgggtcct cctagtaccg ataatgcgca taagctttgt tagcttccgc catcttatgg
      421 gtgtcatcac gacgtttgac ggacgcgcct gttccattag cagcgtcaat aatttccgct
      481 gcgagtcttt cttccattgt tctttcattg cgtaagcgtg aatagttcac taaccaacga
      541 agacctaatg ttgttttacg ctctgggctc acatcagtcg ggacttgata gttttggcca
      601 ccgacacgac gaaccttaag ttcgacctca ggcataatat tactaattgc agttgcaaaa
      661 acgtccattg caggttgttt tgttttcgct tcgatacgtt caaaagcgtt ataaagaatc
      721 gtttgggctg ttccgcgttt cccgtcatac attaatttat taattaagcg ggtaacgagc
      781 tttgagtgat aaattgggtc tggtaagacg tcacgttttg caacacttcc tttacgaggc
      841 atatcgtttc ctccttcctt cgataataag tagtttgttt atttttttat taatgttcag
      901 cagtatgtgg gcgttttgtt ccgtattgac tacgaccttg acgtcttttt tccacaccag
      961 cggtgtctaa tgtaccgcgc acgatgtgat agcggacccc tggtaagtct ttaacacgac
     1021 ccccacgaat taatacggtg ctgtgttctt gtaagttatg tccttctcca ccaatgtagg
     1081 ctgtaacttc ataaccattt gataaccgta cacgagcgta cttacgtaac gctgagttag
     1141 gtttctttgg tgtcattgtt ccaacccggg tacacacacc acgcttatgt gaagaatact
     1201 gattaacttc acgacgttta agtgagttcc agcgtttggt aagggcaggt gattttgatt
     1261 tgtagactgc cttttgacgg ccatgacgca ctaattgatt aattgttggc atccatcttt
     1321 ctcctttctt cgaatcttct tttctgctac tcgacacaca accgggtgta tcaaggtttt
     1381 tgttttaaaa acgaccttta ggtcattgca ctttgatatt atagggacat tgctaaataa
     1441 tgtcaacaca aaactgaaaa ttattcgaga ttgttaattg acaaagtaaa atccgttgtt
     1501 aagaatatgc aattaaaata taaattaact atatgatttt taatgtgaat tataatgctt
     1561 aatatgttta attatcaatt ttttgttcga gaattctttt taaatcaaca tagttaatag
     1621 ctccgcttgc gattccaaag gctatttgtt caatttcgtc atcagtaaaa gaaattataa
     1681 tcccatttat ttgtaaaaaa gaaagcatca catataagcc aattctttta ttaccatcca
     1741 caaacggatg atttgaaata aatccaaagc caagtctagc tgccttttca attttagtcg
     1801 ggaaaagttc aacaccatca aaagtttgat atggtgcttc aattgctgat tctagtagac
     1861 tctcatcacg aataccaaaa actcctcctg tggcctcaat tatgagttta tatagaataa
     1921 tattcgttct ttagtaaatt taatcatttt gctaaatttt taaaggcgtt gatatgttta
     1981 ctaaggattc ttttcgcaac tatttctatc ttttcgtctt ctgttaactc aatatattgt
     2041 tcaatatctg caagaatgta ttttggcttg ttatttttaa atatcacagc ggaaccatat
     2101 ttttccgcag tttttgaagc ttttgaaaaa ttttgatttg catcagtcat tgtaataatt
     2161 tgctctgttt taataatcat tatcgcaccc tcaggataat tatagattat tattaggata
     2221 aattcaacct atttttatta atgtttaaaa aggctggagt caaaccccag cctttagtta
     2281 cataaaacgt tgttttataa tttagtgata acgatgtttt ttactgatta atttaaagcc
     2341 agcaagagca actaatgcca taacgataaa tgcccccatt accagtgcat tgtttgtgcc
     2401 ttcattactt acaagcggag atccactagc agggattgtc cggtcaagga agttactaaa
     2461 cattgcggtt ccatacttct tcacaatata gtcataacgc gccattgctt gcgctaataa
     2521 gtcaccatta gaatcagcaa ccgctgtttt taaatacgct tttgttgtag gatcaagtga
     2581 gtcattatat tgattcgcta gagtttcatt ccattctgga gcatttaaat tatttgctgc
     2641 acctgttgcg tcacatgttg ccgttttttc aagaatctta ctcgcgtata cggtagcttt
     2701 atgatgaact tcactaacaa aagtccatgt tcctgtttct tgatgataga catttttacc
     2761 aatttcccac actatatcgc ccgtttgatt tccagcatta tcgtggaata taacgttttc
     2821 ggtaagatag gcagcatgga aaaaccatga atagtagccg aatccgtgat taaacattgc
     2881 tgttccaggc caattgcctg ttattttgcg accatcaccg tcccatgtat aaagataaac
     2941 atcaccactt gaaagaactt tattcgggtc atagaagatt aaagtagtat cttgtgattc
     3001 aacgtatttt ccgtagagtg ttaaatcagc agttaataga cctggaacat aagcaatcgt
     3061 taattcaggg tcagtgaacc atcccgcata tcctccagtg ccaatcccgt caaatagtga
     3121 ctgattatgt ccagttactg gggtgttaag ccattcttgc caactagtgg gcggggtcgt
     3181 gacgatgtct ttattgtaaa aaagcactga tgttgtcata ttcagtggca caccgtactg
     3241 actaccttta tacaccgttg tcgctaaacc tgaagcaaag aatttagtat tatcaataac
     3301 cgcactatta gcgatatatt cgtctagttt agcaatcgtg tcgtcgtgcg cataatcact
     3361 aatccgtggt tgatcgaaat aagctaaatc tggtttactg cgtgaactta tcgccgcatt
     3421 aagtgatttg ttataactat ctttaacgac catcagtaat ttaactttaa ttcctgtttc
     3481 cgctgtaaag tcagcaatta aatttttata cgctgtttgg tctgtttgtt ttgaaggctg
     3541 ataggcccaa aagttaattg ggtcatcact cccaccattt tcactaccat ctttacagcc
     3601 agtcacaaca ctgagtgata aagccaaaaa aagactaaat aagattgttg ttattttctt
     3661 cggtttcctt ttgtgcacct ctataaaatg ttatcgttat cattttaaaa tataattgct
     3721 aattttgcaa gttaaattga aattctatgt aatagctaga ttgattcgcg atgaataatc
     3781 gttgtttcaa tgaatgcgtg attaactggt aattcatttt tattttcaat cagggcaata
     3841 atgcgatggg caagtgcttt tccggcttcg taaattggtt gatgaatggt agtaagtcga
     3901 gggtgcgcta cttcttgggt acctaaccca tcaaacccag ttactgctat gtcacgcggt
     3961 acttctaaac ctttttcttt aattgcgcga atagcaccaa ttgccatatc atctgtagcg
     4021 cacacaatta cttcaggagt atcaccttgt tttaaacttt gacccacgac tttataagcg
     4081 tcattttcat tatttttagc ggccaaatat tttacagtaa ttttatcttt ttgactcata
     4141 actgcgtcac taaatccttt tttacgctgt aagataaacc gtcttgagtc atcaattcct
     4201 aaatatgtca gtgttcgata gcctttacta agtacgtgac ttgtcattaa gtaaagccct
     4261 tgataattat caacgtcaac acttgacact ccttctacat agccaaagac gcacactggt
     4321 ttagtttttg aaagttcaaa tacctttgct tcatcttccg cgctaagtcc catgaaaata
     4381 cagccatcac aatcttcgcc ctcataccac gatcttttta agagtaagga aaaacctttt
     4441 tcgccaagta cttcgcctat accagacaca acatgtaaaa agaacgggtt aatcgcatca
     4501 atatcagcgg gaatatacac ataaatgata ttcgtgcgat tttggacgag tgctctagct
     4561 aaaacgttcg gcttaaaatt ttgccgcgct atttctgctt caataatctt ccgtgttttt
     4621 tctttcacta aatgcggtga atttatgaca cgcgaaacag taataacaga aaatccagta
     4681 gcttttgcaa tatcttttaa tgtagccata tgttttattt tatcattatt tgattaataa
     4741 aaattacaat acaaaacgac aacttaagct ttacgaattt taatggtaac cccttcgcta
     4801 ttcatttcac caatttcaat actataacca attacgctac cgtcattaaa ggttgctctt
     4861 tttaacaagt acttttgcca attattacta gcaataactg tccctgtttg aaatagcgct
     4921 gagtcgtccg cttttactaa attatttgcc catgtttgcg cgcgtccgcg accatcaagc
     4981 aaatgcaatt tatgtaaact gttatgcgca taattataac ttggtgtatt ggaagtcgca
     5041 aaatccatcc atgaaccacc gctaccatct aagtgcggcg gaattgtatc actccacttt
     5101 ttagtattac cgttatttga caaaatcact aaccgcgcat caacatgcca tatccgtacc
     5161 ccaggaatcg tgtaaccttt gacaccgtta tagcctgaaa ctgataagct atcacgcgca
     5221 tttaaaccgg ttggtgtgta gtactcaatc aaaatgtatt cgtcataagc atgtccattc
     5281 caagcgtcat taataatgat aaagtcacca cttgattcag cgggtctaag ccttatatca
     5341 ctattaccaa ccgggacata cggtgtgctc cagtttgcgc taaatttaga aaacatattg
     5401 tgatcaataa tattatgatc catcatatca atcgctcccg ctggcgctat agtgccgtca
     5461 taatcataat aatcatcaaa acttaagata tgaccacttt catgaatata agtatgcgct
     5521 gctggtttaa gatatgtata acctggcgct tcaaccataa attcataact cgcccagccg
     5581 taagcattaa atactggttt gtttttatcg gcaaatctat tccaaaaata ttggaaggcc
     5641 caaaaaagtg tatcatcact ttcataatca acaggtgcta agtacacaaa gaaaacggaa
     5701 tcaacaaagc catcattatt ttggtcgtat tcttttaatg ttgatgaaag ataagtgttg
     5761 tacacctgtt caagcacata gtgactctta tcccagtact gataaccgtc aacagttttc
     5821 gctaacaatt cagtagtgga ataacccata tcaaaaaggg gcattacttc accggtaaga
     5881 ctgagtctac cataacttga tttatagtaa taagaggcta ctgattccca acctgtatca
     5941 ctgctttcac caaagaaagc gcgattaatc gctccctgaa tagtcgcctc tcccccgtaa
     6001 cttgcgggtg tatcagtaaa tttaacaggt acaaccatca ttttttggtt agaaactgaa
     6061 tcaaggtgcg ctaaacccat cgtattagaa atagcgtagt tattaattag tcgcgaatta
     6121 ttgttaagcg gttctacttt ataataatta tttaaagtat ctggattcgc cgtaattggg
     6181 tgttcataat cattaacgcc gtatgtcccg tttaaaggat agggattttc gctgccagtt
     6241 acttcggaag ttacttcatt cgcacttgaa ctagtcgcac caaactcgga cgactttaat
     6301 atatcaccaa aagtcacgca gcttgttaag agtagagctg ggactactaa aattaatgct
     6361 cgacggataa atgtactttt tctcatctta atatacctct atttctattg tcgcataagc
     6421 gttagttaat tgtactattt taaagcgaaa attaagcggt gtcttatcat taaatgcgaa
     6481 atttttaaaa tattcatagc caaactcatc acctttttga aacaaagagt gctgatttgc
     6541 ttgattaaac gactcgctga tgttcgtgct ttcttttggc tcaagtaatt tataaagttt
     6601 attcgtctta attggtgcat tattataact tccagtacta ttgctatgcg ctaaaagttc
     6661 agtaccgtta aattctcctt cataatcatt aacgtaagga tacttaacaa tactcccgtt
     6721 tttagtttcg tacaccgttc tcgcgtcaac gtgatacacc ttcactccgt ttgtctttgg
     6781 cagtttaaag gaagcattaa gcgttgaatc aagctcattt aacactgttg gtgtgtaata
     6841 ttcgagcaaa atatattcat catttgcttt ctcgttccag ttatttttaa gaacgagtaa
     6901 gtcacctgtt tcactaaagg gtcgaagttt aattgtagta cttcttttta aggcgtaagg
     6961 ccgcgcccaa tcaagtaaca tctttgataa cccggtatga tcaccaagcg tataatccat
     7021 catatcaaag ccgccaagcg gggaataagg atcgtcataa ttcgtattat aataatcagg
     7081 taaaccaaaa agatggccta cttcatgaat ataagtatgg ggatcaggtt ttttattgcg
     7141 cgctacatct aaataataat aactactcca act
//
LOCUS       DAOPCD010000027         7008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_9642_length_7008_cov_3.822523, whole genome shotgun sequence.
ACCESSION   DAOPCD010000027 DAOPCD010000000
VERSION     DAOPCD010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9642_length_7008_cov_3.822523"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PLQ55_01450"
     CDS             complement(<1..1241)
                     /locus_tag="PLQ55_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPK28637.1"
                     /translation="MIKKVNITIDNKKLEVDSNITVLQAATDADINIPRLCFLKGISE
                     TSSCRICVVEDVGRNRLVNSCTYKVYEGLNILTTSPRVIKQIKINLELIAGNHQFDCW
                     ACSREHNCELLELLRRFNIDNHYNINKEIKKREWIVNKSESMVLDSSKCILCGRCIDA
                     CNHYTGLGILGFNNRGFMTSVGPAGGVNIGEAGCIYCGKCIQSCPTGALREKDDVDRA
                     FDLLDEEGAYVVAQIAPSVRATLGEEFGYPMGTNVEGKIYHALEKLGFHDVVDVNFAA
                     DLTIMEEGTELIGRLKAFLDGDKDVKLPLFTSCSPGWIRYIERYAPEYLGHVSSCKSP
                     QGMSGAIIKNYYAQKLGIEREKIKVVSIMPCIAKKYEASRPEMEVDGVRDIDLVLTTR
                     ELARFIKRHVIDFDMLTNKQLT"
     gene            complement(1253..3046)
                     /gene="nuoF"
                     /locus_tag="PLQ55_01455"
     CDS             complement(1253..3046)
                     /gene="nuoF"
                     /locus_tag="PLQ55_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPK28638.1"
                     /translation="MHKHYEIVLCGGSGCVSSKSQLIKQKFEEILARENLSDFVKVYM
                     TGCFGMCEKGPVVVVYPDQTFYAKVKITDVEEIVESHIKKGLIVDRICHHEFDEKLQK
                     MVYKNLHEMKFHSAQKRLALRNCGVIDPDEIDEYIGRDGYFALEKALRMEPKEIIQLI
                     TDSGLRGRGGGGFPTGLKWQLAERFRGGQSYVICNADEGDPGAFMDRSILEGDSHAVI
                     EAMIINGYAIGANQGFVYIRAEYPLAVKRLQNAINQAYKYGLLGKNIMNSGFDFDLEI
                     RLGAGAFVCGEETALISSIEGRRGMPRNKPPFPAEHGLWGKPTIINNVETYANIPQII
                     LNGPEWFQKIGTERSKGTKVFALGGKINITGLIEVPMGTTLREVIYDIGGGIPNGKKF
                     KAVQTGGPSGGCLTEEDLDTPIDYDTLIQKGSMMGSGGIIVMDEDNCMVDVAKFYLDF
                     IVDESCGKCTPCREGTRRLYELLEKITEGKGTLETLEELKQLCYDVKDTSLCGLGQTA
                     PNPILSTFNKFYDEYVEHVIDKKCRAGVCKSLTTLYITDKCIGCTKCVRNCPVNCITG
                     ELRKLHRIDTTKCIKCGACIQNCPVHAIIHV"
     gene            complement(3049..3414)
                     /locus_tag="PLQ55_01460"
     CDS             complement(3049..3414)
                     /locus_tag="PLQ55_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28639.1"
                     /translation="MIKTKRDLEVIKKRMQPDFHLRKEEHKYRLLFRYNEKTVIQNIE
                     KIIDLCLAAIYQDKLDAVAIKVYDTRVEDKLILVVKEKNRAVRYNIKNEAMVTEILNK
                     HIGQGKVVYEYVDVIDKEA"
     gene            complement(3395..3904)
                     /locus_tag="PLQ55_01465"
     CDS             complement(3395..3904)
                     /locus_tag="PLQ55_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014969041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPK28640.1"
                     /translation="MCQKTFEEKLAELDACIEKHQKSPGPLMPTLHDAQEIFGAIPLE
                     IQKHISKKLGVSVAHINGVVTFYSRFSLTPKGKYIISVCMGTACYVKGAQAILDAVQS
                     ELKLKPGETSEDGLFTLEATRCIGACALAPVYSINDEIYGGGTSKKILEDLRKIIKAE
                     EEKNDQNKA"
     gene            complement(4062..4915)
                     /locus_tag="PLQ55_01470"
                     /pseudo
     CDS             complement(4062..4915)
                     /locus_tag="PLQ55_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214884.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
     gene            complement(5091..5282)
                     /locus_tag="PLQ55_01475"
     CDS             complement(5091..5282)
                     /locus_tag="PLQ55_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPK28641.1"
                     /translation="MINYAQKIREYRERKFLTQTEFAALLGVSIASVTRWETGHYQPT
                     MKVKKKIYNLLVEAGIEIE"
     gene            complement(5390..5474)
                     /locus_tag="PLQ55_01480"
     tRNA            complement(5390..5474)
                     /locus_tag="PLQ55_01480"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5438..5440),aa:Leu,seq:tag)
     gene            complement(5527..6885)
                     /locus_tag="PLQ55_01485"
     CDS             complement(5527..6885)
                     /locus_tag="PLQ55_01485"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HPK28642.1"
                     /translation="MRITMLEKLKMCKLHIEKKMSLSHISEMYGGYHVANIKYAVNLY
                     KRHGEGPFLDRQRRIYKRDTKLLAIGQVLSGRSLRDVGVEHALTDPGIVSDWVKKYKT
                     EGEDAIQDTYPRANYLLKEERFKAVVDKKVEAENERLRAEIAYLKKSPSLVRRLEGAT
                     VQERVRIVEELRTKHNFKTLLEISKLASSVYYYTVNAAKKKRNKYIDIEKEIDRLYIK
                     VHKKRCGYHQIYNNLKNDGWTIGKNKVLEIMRMKGYTKTKKNKWRKYNSYEGNLGNNI
                     GNLMNQDFSTRKPYEKAGTDVTEFPLLGTSVYLAPVIDFHTREILAYTVGTDAKVERI
                     MVMLKAMKKKHGNKLKGMMLQSDQGVQYQNSRYIQKLKKMGIIQSMSRKGNCLDNSPT
                     ENFFGRLKQEIWDDNKEDYDSVESLITAIHEYVSYYNNDRFVMKTKLPPIVCRNNYYL
                     SI"
BASE COUNT         1978 a         1539 c         1017 g         2474 t
ORIGIN      
        1 gttaattgtt tatttgttaa catatcaaaa tcaatgacat ggcgtttaat aaaacgcgct
       61 aattcacgag ttgtgagtac taaatcaata tcacgaacac catcaacttc catttctgga
      121 cgacttgctt cgtatttttt agcaatacac ggcatgattg acacgacttt aatcttttcg
      181 cgttctatcc ctaatttttg cgcataataa tttttaataa tcgctccgct catgccttgt
      241 ggtgatttac atgaagaaac atgtcctaag tattcaggag catagcgttc aatataacga
      301 atccagcctg gtgaacagct tgtaaaaagt ggtaacttca catctttatc accgtcaagg
      361 aatgctttta aacgcccaat taattcagtg ccttcttcca taattgttaa gtcagcggca
      421 aagttaacat ccactacatc atggaaccca agtttttcta atgcgtggta aattttacct
      481 tcgacattag tacccattgg gtaaccaaat tcttcaccta atgttgctct aacacttgga
      541 gcaatttgcg caacaacata agcgccttct tcatcaagta agtcaaaagc gcgatcaaca
      601 tcatcttttt cgcgtaatgc tcctgttgga caactttgaa tacatttacc acaatatata
      661 caaccagcct caccaatatt cacaccacca gcaggaccaa cacttgtcat aaatccgcgg
      721 ttattaaaac caaggatacc taatccagtg taatgattac aggcatcaat acagcgccca
      781 cataaaatac atttactact atcaagcacc attgattcgc ttttattaac aatccattca
      841 cgttttttaa tttctttatt aatgttgtaa tgattatcaa tattaaagcg ccgtaataat
      901 tcaagtaatt cacagttgtg ttcccgtgaa catgcccaac aatcaaattg gtggttacca
      961 gcaattagtt ctaagttaat tttaatttgt ttaataaccc gtggtgaagt tgttaagata
     1021 tttagtcctt catatacttt ataagtacat gagttaacaa gccgattacg accaacgtct
     1081 tcgacaacac aaatccggca tgatgaagtt tcactgatac ctttaaggaa acaaaggcgc
     1141 ggaatattaa tgtccgcatc agtagccgct tgtaaaacag tgatattact atcaacttct
     1201 aattttttat tatcaattgt aatatttact tttttaatca taatcattac ctctatacat
     1261 gaatgatggc gtgtactgga caattttgaa tacacgcacc acatttaata cattttgttg
     1321 tgtcaatccg gtgtaacttc cgtaattcgc cagtgataca gttaactgga caattgcgca
     1381 cacacttcgt acaaccgata catttatcgg taatataaag tgtcgttaat gatttacata
     1441 caccagcgcg acatttttta tcaatgacgt gttcaacata ttcatcataa aacttattaa
     1501 acgtactaag aatcggattt ggcgcggttt gacctaaacc acaaagcgaa gtatctttaa
     1561 cgtcgtaaca aagttgtttt aattcttcaa gtgtttcgag tgtaccttta ccttcagtaa
     1621 ttttttctaa aagttcataa agacgtcttg tgccttcgcg acacggtgta cacttaccac
     1681 aactttcatc aacaataaag tcaagataaa acttagcgac gtcaaccata cagttatctt
     1741 cgtccatgac gataataccg ccggaaccca tcattgaacc tttttggatt aatgtatcgt
     1801 aatcaattgg tgtatctaaa tcttcttcag ttaaacagcc accacttggt ccgccagttt
     1861 ggacagcctt aaatttcttg ccattaggaa ttccgccacc gatatcataa ataacttcgc
     1921 gtaatgttgt gcccatcggt acttcaatga gcccagtaat attaatttta ccgcctaagg
     1981 caaacacttt tgtaccttta gaacgctcag tgccaatctt ttggaaccac tctggaccat
     2041 ttaaaataat ttgcggaata ttagcgtaag tttccacatt attaataatt gtaggttttc
     2101 cccataaccc gtgttcagca gggaatggcg gtttattgcg cggcatcccg cgtctacctt
     2161 caatagatga aataagggcc gtttcttcac cacagacaaa agcaccagcg ccaagtctaa
     2221 tttctaaatc aaaatcaaac ccgctattca tgatattttt gcctaataaa ccgtatttat
     2281 aagcttgatt aatcgcattt tgtagccgtt taaccgctaa aggatattca gcgcgaatat
     2341 aaacaaatcc ttgatttgcc ccaatagcgt aaccattaat aatcattgct tcaatcacag
     2401 cgtgactatc accttctaaa attgagcgat ccataaaggc acctgggtca ccttcgtcag
     2461 cgttacaaat aacataactt tgtccaccac ggaaccgttc cgcaagttgc cattttaacc
     2521 ccgtggggaa cccgccccca ccgcgccctc ttaagcctga atcagtaatt aattggataa
     2581 tttctttagg ctccatccgt aaagcttttt caagggcaaa atagccatca cgcccaatgt
     2641 attcatcaat ttcatcagga tcaattaccc cacagttacg gagcgctaaa cgtttttggg
     2701 cactatggaa tttcatttca tgtaaatttt tatacaccat tttttgcaat ttttcatcaa
     2761 attcatgatg acaaatacga tcaacaatta aacctttttt aatatggctt tccactattt
     2821 cttcaacatc agtaattttt actttcgcgt aaaatgtttg atcaggataa acgacaacaa
     2881 ctggtccttt ttcacacatg ccaaagcatc ctgtcatata aacctttaca aaatcgctta
     2941 agttttcgcg ggcaaggatt tcttcaaatt tttgtttaat taattgactc tttgaggaaa
     3001 cacatccaga accaccacaa aggacaattt cataatgttt atgcataatt aagcctcctt
     3061 atctatcacg tcaacgtatt catatacgac tttaccttga ccaatatgtt tatttaatat
     3121 ttcagtaacc atcgcttcat ttttaatgtt gtaacgaaca gcacgatttt tctctttaac
     3181 aactaaaatc aatttatctt caacgcgtgt atcataaact ttaattgcta ctgcatcaag
     3241 tttatcttga taaatagccg ctaaacagag atcaattatt ttttcaatat tttgaattac
     3301 tgttttttca ttataacgaa acaataaccg atatttatgt tcttctttcc gtaaatgaaa
     3361 atctggttgc attcttttct taattacttc taaatcacgc tttgttttga tcattttttt
     3421 cttcctctgc tttaattatt tttcgtaagt cttcaagaat tttcttactt gtcccaccgc
     3481 cataaatttc atcattaatt gaataaacag gggcaagcgc acatgcacca atacagcgcg
     3541 ttgcttcaag cgtaaataaa ccgtcttcac tagtttcgcc tggtttaagt tttaattcac
     3601 tttgcacagc gtcaagtata gcttgcgcgc ctttaacata acacgctgtc cccatacaaa
     3661 ctgaaataat gtattttcct ttcggggtta aagaaaaacg tgagtaaaat gtcacaacac
     3721 cattaatgtg ggcgacactc acgcctaatt tcttactaat atgcttttga atttctaagg
     3781 gaattgctcc aaatatttct tgagcatcat gtaatgtggg catcagtggc cctggactct
     3841 tttgatgctt ttcaatacaa gcatcaagtt ccgcaagttt ttcttcaaat gttttttggc
     3901 acatattcgt ctcctatcgg tatttatttc cagatacaac tatgattata atatgtatat
     3961 tataaattca aaacaatatt ataaatgttt ttttaatgag acattgaaaa atcttagata
     4021 ttcttaaaaa gcgtacatgt caattacaac gcattatctt attatttttc attatcatca
     4081 tcattttttt cggttatagc ggtaattact cttcctaaat caagtgcttt tttactcgtt
     4141 ataacttttt tccagttttt gactcaattg cttcgcgcgc cgctttggca acactcccac
     4201 ccacatttgc tatctcgata ttttcttcaa gtccatcagg atgctttaca ttaataattt
     4261 ctttagtggc cgtttctgca agcatgttta acacaatttc tgttgttgac atattatctc
     4321 gtaaactttc ttttgttaaa ccttttagat ttttatattg cctagttgtc attccactcc
     4381 aggcttttgt aatttcattt cttaatattg cgtattctga accagcttta acaccatgat
     4441 cttgccacgc atctgttaac tctttacgta ccgatattgc ttgtaatctt tgattaatcc
     4501 attcacgggt gtaacctttt gctaaatatg tgcgtaaagc acgatcaata gttagctctg
     4561 gatccattat ttcatcaacc cgctcttttc ctacttccgt taaccacatc ttgaaaggct
     4621 cggctttagg cgatggaaca gattgtatga tgcgaaaaat tcctcgtata tcagcgatat
     4681 cagtgttacg cattttccca tctgatgctt tcaatttcaa agcgtgacaa aatgtcacgg
     4741 tttcacttcc gtcttctttt aatcgctgtt ttagctttct ccagtatgct ccggggtctt
     4801 tgctctctgt taagacgcca atgatatcta caacactaaa atactattct tctttttctt
     4861 catcccaatg tgttctaatt tgtttgcctt caaataattt aatttcgttt ttcaaaactt
     4921 aatttcatat tatctaatcc tacccggttt aattaatgat tagataatat tctcctcttt
     4981 ctccgggtat aacaattaca ctatttactt gttaatttta cctacagcct tgtggagttt
     5041 ttttactata ctcttatctt ttttcttaac ctctaatagt taatctcctt ttattctatt
     5101 tcaattccag cctcaactaa taaattgtaa atttttttct taactttcat tgttggctga
     5161 taatgtcctg tttcccatct cgtcactgat gcgatagaaa caccaagaag cgccgcgaat
     5221 tctgtttgtg tcaaaaactt tcgttcacga tactctctta tcttttgggc ataattaatc
     5281 atgataaact cctatttaaa ctatttacac attattttat cactttttta tcactaacgc
     5341 tattggaatt aataaaaaat gccccgttta aggagcaatt taagtaacgt ggtgcaaacg
     5401 acaggaattg aacctgcatg ggtttcccca ctagatccta agtctagcgc gtctgccagt
     5461 tccgccacgt ttgcatgaat tagcttccaa atcttggaga gaaataaatc tcaacccaca
     5521 cctttattat atacttaagt aataattatt tctacatact atgggtggta actttgtttt
     5581 cattacaaac cggtcattat tgtagtaact cacgtattcg tgaatcgctg ttattagact
     5641 ttcaacacta tcgtaatctt ctttgttatc atcccagatt tcttgtttta gccgcccaaa
     5701 gaaattctca gttggtgaat tatcaagaca atttcccttc ctcgacattg attgaataat
     5761 acccattttc tttaattttt ggatgtatct tgagttttga tattgcactc cttgatctga
     5821 ttgcaacatc attcctttta gtttattccc atgtttcttt ttcattgcct taagcatgac
     5881 cattatccgc tcgaccttcg catctgttcc taccgtataa gcgagaattt cccgcgtatg
     5941 aaagtcgata accggggcca agtagactga tgtccctaac aatggaaact ctgtaacatc
     6001 agtcccggcc ttctcatatg gcttccttgt actaaagtct tgattcatta ggttaccgat
     6061 gttgtttccg agattcccct catacgagtt atactttcgc cacttgtttt tcttcgtctt
     6121 tgtataacct ttcattcgca tgatttctaa cactttattc ttgccaatcg tccaaccatc
     6181 attcttcaaa ttattgtata tttgatggta tccacaccgc tttttatgta cttttatata
     6241 taaacggtcg atttcttttt cgatatcaat atatttgttt ctcttttttt tcgccgcatt
     6301 taccgtgtaa taatacactg atgacgctaa tttacttatt tctagaaggg ttttaaagtt
     6361 atgcttcgtc cttagttcct cgacgattct tactctttct tgaaccgtcg ccccctctag
     6421 tcttctcact aaggacggcg atttttttag gtatgctatc tccgcgcgga gtcgctcatt
     6481 ctccgcttct accttcttat ccaccaccgc tttaaaccgc tcttctttca ataaataatt
     6541 cgcgcgcggg tatgtgtctt gaattgcatc ttcgccctcg gttttatact ttttgaccca
     6601 gtcactaaca atccctggat ctgttaatgc gtgctcaacc cctacatcac gcaaggatct
     6661 cccacttaaa acttggccaa tcgctaggag ttttgtatca cgtttatata ttctcctttg
     6721 ccgatctaaa aatggtccct ctccatgtct cttataaagg tttactgcat actttatatt
     6781 tgctacgtga tagcccccat acatctctga aatgtgtgaa agtgacatct ttttttcgat
     6841 gtgtaattta cacattttca acttttcaag catcgttatt cgcataaaaa atagctccct
     6901 tcttgggtgg gtatttctat tatacctctc tccaagattt ggaagctaat tcattgataa
     6961 ctggtgcagc cagtgagact tgaactcaca cgggattacc atacgccc
//
LOCUS       DAOPCD010000028         6504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_10489_length_6504_cov_4.320670, whole genome shotgun sequence.
ACCESSION   DAOPCD010000028 DAOPCD010000000
VERSION     DAOPCD010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10489_length_6504_cov_4.320670"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PLQ55_01490"
     CDS             complement(<1..702)
                     /locus_tag="PLQ55_01490"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPK28643.1"
                     /translation="MKIFVLGNINSGKSYVVSKLKKIFPDYPVLAIDEYRINHSNGSI
                     DSELKTRNLFANDILKHNNAIIEYSGGSMITNLFINELKINSFIIIEVTEDVNVCLKR
                     IQNKDFSKIPYPIFEESLEDTIIRLDKEFKNDSVKTNFKDKFLHRFKISSIDDLSILP
                     LKQYAEALKISNHLQNGYKAIVLYGSLGRHKMNQNSDLDIFLYTDKSINAVYEDLLFI
                     YSDSKFLFLKNQIVIY"
     gene            complement(699..2036)
                     /locus_tag="PLQ55_01495"
     CDS             complement(699..2036)
                     /locus_tag="PLQ55_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HPK28644.1"
                     /translation="MKKIINSLKTINYKMFFALFIFGLIPTLYTTFRIYLIGQLPNTY
                     GFSIAGQLQWVSLLYEILQEALILPLFYFIGAVISEKEALINRIRSGLIFTFIVYTVL
                     SALIFIFARPLVIFMAQDNSIIDETVNYIRLETIAMIFGTLMKYLMVVLVTIKKDKNI
                     YIVLIAQLILTILLDTFFVSTLKVSLNLGINGIPITNIIVNFLMVIFLVIILYKNELP
                     IFNSFKSDYSWFKDLFKKGGISGLESFVRNIAFMLMIVRMVNVVGEQGTFWVANNFIW
                     GWLLLPVLQLGELIKSDCGEDGMKAVQKKSFGYFNLTIIFVFIWAITLPLWKPFMQHV
                     LQIPNYKDVHFIVLISVGFYVLFALNNVIDSIFYGLGKTNLMLFQSLVINTIFYGLMF
                     ILFQTGVFQPNLTLIALMFATGTALDSILTMILYAIMLKRERINILNFEEAIA"
     gene            2981..3487
                     /locus_tag="PLQ55_01500"
     CDS             2981..3487
                     /locus_tag="PLQ55_01500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPK28645.1"
                     /translation="MELRDKLRTDPLTFVANRNFFDFQINKRLEEARLFGNKFGILMM
                     DIDNFKKVNDTYGHLVGDEIFKIVAYTLSSNVKKTDMVSRWGGEEFIALVDVTTNEEL
                     AAIAERLRTLVAASGYKLNVDKTIQVTISIGGTLVTKDDTIKSAITLADDNMYYAKQN
                     GRNQTKIA"
     gene            3660..5069
                     /locus_tag="PLQ55_01505"
     CDS             3660..5069
                     /locus_tag="PLQ55_01505"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HPK28646.1"
                     /translation="MIKITYDNKFTTNLVLVLDETPKIKELELMLEQKLFNAKVGEVF
                     VSVNLNREGNIYLGLEKGLAEDHEKIRTAGYTLAQKLVSEKISKINLDCNEVDPKLIS
                     TLVQGLLFSNYNFDKYKSTKKEDFNVEVSLLNAPTSLKAKIKELEGVIRGVNVARDLV
                     NTRAIDLYPETYATKIVELFKGTAVEVEVFDKKEIEKLGMHAFLAVNAGSDKEPRFVV
                     MKYFGNKTSDKHITFVGKGLTYDSGGYDIKPGKSMFEMFTDMAGSAAVIGAIKAIADN
                     NLNANVVAVTALTENLINGKAYKNGDIISTMKGSTVEIGSTDAEGRLTLADAIYYAAT
                     KLNSTHIIELSTLTGACVVALGTDMIGATTNNEEFYNKVFDAGNHVGEPSWRLPVTDV
                     LKEAVKGEFADLKNSVPGGAGTITAGIFLEHFSENIPFVHLDIAGTAYGKAKRYYANG
                     ATGVGVRTLYKLVATNFSK"
     gene            5347..5970
                     /locus_tag="PLQ55_01510"
     CDS             5347..5970
                     /locus_tag="PLQ55_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28647.1"
                     /translation="MNLERAKEKFGVREPFYKNELKDFLKIENQNTFNQLLSNMNKFN
                     VVKKMENGIYYFPSANEHFKELKPSVSSVVNLKYLKNYNGIRTGAFLAYKYKLTSQVS
                     GYYEIITNNVSEHTRAKKEYDGKVIVSSSKFNIDKNNYLYAEFLELVNNMNYSDYDYK
                     KTLNLIIEVFKEMNLDIQKTKKLAKNYRGSVYASYLRKVQDILNEVA"
     gene            6101..6484
                     /locus_tag="PLQ55_01515"
     CDS             6101..6484
                     /locus_tag="PLQ55_01515"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPK28648.1"
                     /translation="MQCAVKRFAESKNNGLCLIDMPTGTGKTYLTGKLIEDYIKGNIL
                     SDIDTIIYLTPLKKNVDDIYEDVRKAFSDDAELFDNNALRLFPNYECVIENITEEVIL
                     SKIPQSLKKKESFKNPSLGLYTRKV"
BASE COUNT         2480 a          906 c         1018 g         2100 t
ORIGIN      
        1 ataaataaca atttgattct ttaaaaataa aaacttagaa tctgaataaa taaataataa
       61 atcttcataa actgcattaa ttgatttatc agtatacaag aagatatcaa gatctgaatt
      121 ttgattcatt ttatgtcttc caagagaacc atataaaaca atagctttat atccgttttg
      181 aagatgattg gatattttta aagcttcagc atattgtttt aaaggtagga tacttaaatc
      241 atcaatactt gatattttaa atcggtgtaa aaatttgtct ttaaaattag ttttaacgct
      301 atcattttta aattctttat ctaatctaat aatagtatct tctaaagatt cttcaaatat
      361 aggataagga atttttgaaa agtccttatt ttgaattctt ttaagacaaa cattaacatc
      421 ttcagtaact tcgataataa tgaaagagtt aatttttaat tcattaataa aaagatttgt
      481 gatcattgat ccacctgaat attcgataat ggcgttatta tgctttaaaa tatcattagc
      541 aaaaaggttt cgagttttta attcagaatc tattgatcca tttgagtggt taattcgata
      601 ttcgtcaatt gctagcactg gataatcagg aaatatcttt ttaagtttgg atacaacata
      661 acttttccca ctattaatat ttcctagcac aaatatcttc atgctattgc ctcttcaaag
      721 tttaaaatat taatcctttc gcgttttagc attattgcat aaagtatcat tgttaagata
      781 ctatctaaag cagttcctgt tgcaaacatt aatgcgatta atgttaaatt aggttggaat
      841 actccagttt ggaaaagaat aaacattaat ccgtaaaata tagtgttaat aactaaagat
      901 tgaaataaca ttaaatttgt ttttccaaga ccgtaaaata tgctatcaat tacattatta
      961 agcgcgaata aaacataaaa gccaacactt attaatacga taaaatgaac atctttataa
     1021 ttaggtattt gtagaacatg ttgcataaat ggtttccaaa gtggtaatgt aatagcccaa
     1081 ataaatacga aaatgattgt taaattaaag taaccaaagc ttttcttttg aactgccttc
     1141 attccgtctt ctccacaatc agatttaatt aattctccaa gttgaagtac tggaagaagt
     1201 agccatcccc aaataaagtt attagctacc caaaaagttc cttgttcgcc aacaacatta
     1261 accattctaa caatcattag cataaacgct atatttctaa caaaactttc taatcctgaa
     1321 ataccacctt ttttaaataa atctttaaac caagaatagt cacttttgaa tgaattaaat
     1381 atagggagtt catttttata tagtataata actaaaaata taaccattaa aaagtttaca
     1441 ataatattag taattggaat accattgatt ccgagattca gcgaaacttt taatgttgaa
     1501 acaaaaaagg tatcaagtaa aatagttaga attaattgcg ctatcaatac aatgtatata
     1561 tttttatctt ttttaatagt aactaaaaca accatgagat atttcattag tgttccaaat
     1621 atcatagcaa tagtctctaa tctaatataa tttaccgttt catcaataat agaattatct
     1681 tgtgccataa aaattactaa aggtctagcg aaaataaaaa taagtgcgga taataccgta
     1741 taaacaataa aagtgaagat taatccgctt ctaatacggt ttattagtgc ctctttttca
     1801 gatataacag caccaataaa ataaaatagt ggcaatatta aagcctcttg taaaatttca
     1861 tataaaaggc taacccattg cagttgtccg gcaattgaaa atccataagt atttggtaat
     1921 tgtccaatta aatatatcct aaaagtagtg tatagtgttg gaatcaatcc aaaaatgaat
     1981 aaagcaaaaa acattttata atttatagtt tttaaagaat taataatttt tttcataatt
     2041 ttttctccta tataatatta atccctttta ctgtcaaaat aattaaatta tttattgaca
     2101 ataactttta agttttgaca attttatagt cacatatttc tttaaaatgt ctcgaaatag
     2161 tatgcttgaa tgtcaaaaca aaacaaacat gaaaacctaa aaattgttaa catttatggt
     2221 tttctctttt aaatactgca aataacatct gcagtaaaaa acaaaatgcg cctattgagg
     2281 cgcataaaaa tcacaaaaaa acttttgcat attatgtctc tcctttcgtc tgttttgaaa
     2341 gggaagttaa tcactacaaa taatacttga tgcattatcg gtaaaggatg aatgccgaga
     2401 catacagcat aagccctaaa cctttggaga taatgattaa cttccatcat tagtttatta
     2461 ttattattta tgaaatgcaa gagttggcga gaaaaatata aaatcaattt tcctctaaaa
     2521 tacctcaaaa gtactccgtt ttccctttga tggacaaaaa gcaccagttt aggtaaaata
     2581 cccaattgtc cgttggaata cctcaaaagt atataagaat gccctatttt ccctttggtg
     2641 gacaaatgtt agcagtttcg cacagttagg acattaacca gtggtccctt taaggtaaaa
     2701 catggctcca atagtgaata aagtccgcat acaagcgggc ttttattttt gctgacatag
     2761 atatagtgta actaatttta atatttgttg ttgcttatgt gccaaaaata tataagaaca
     2821 ctggcacaaa gacaacaaat gaattaatag cctaaaatag acataaaata aaaagcgtaa
     2881 ctaagtgtaa aaaacaataa ctgcttaata ttaacaatgc attaaaaaat gtattagttc
     2941 aaaattatcg gcaaaataaa tttaaaaaac atattaaaat attgaattaa gagacaagct
     3001 ccgtactgat ccgcttacat ttgtcgctaa tcggaatttc tttgatttcc aaattaataa
     3061 acgcttagaa gaagcacgtt tatttgggaa taaatttggg atattaatga tggatattga
     3121 taactttaaa aaagttaatg atacatatgg ccacttagtg ggtgacgaaa tttttaaaat
     3181 tgtcgcctat accctttctt caaacgtgaa gaaaacagat atggtaagta gatggggtgg
     3241 cgaagaattt attgcgcttg tcgatgttac cactaatgaa gaattagccg ctatcgcgga
     3301 aagattacgc acactagtcg cggcttctgg atataagctt aacgttgata aaacaattca
     3361 agtcacaatc tcaattggtg gcacactagt gactaaagac gatacgataa aaagcgcaat
     3421 cacactagcg gatgataaca tgtactacgc aaaacaaaac gggcgtaatc aaacaaagat
     3481 tgcttaaata agtaaaaata taaatatttt ttaacaacag caaaatgaca aaaagggaat
     3541 tgaactccct ttttgtaata aaacatttag gaaattaacc ccagttatga ctaaatcgga
     3601 cgcattttaa acaaagacaa atgataaata tgctacattg tgaagtggag gaaaagaaaa
     3661 tgattaaaat aacttatgac aataaattta cgacaaactt agtgttagtt ttagacgaaa
     3721 caccaaaaat aaaagaactt gaattaatgc ttgagcaaaa gctatttaat gccaaagtgg
     3781 gtgaagtttt tgttagtgtt aatttaaatc gtgaaggtaa tatttacctt ggacttgaaa
     3841 aaggcttagc ggaagaccat gaaaaaataa gaactgctgg ctatactcta gcgcaaaaat
     3901 tagtgagtga aaagattagt aagattaatc tagattgtaa tgaagttgat ccaaaactca
     3961 tttcgacttt agtgcaaggc ttattattta gtaattataa ttttgataaa tataaatcaa
     4021 ctaagaaaga agactttaat gtagaagttt ctttgcttaa tgctcctaca agtttaaaag
     4081 ctaaaattaa agaattagaa ggagtaatta gaggtgttaa tgttgcgcgt gacttagtaa
     4141 atacgcgcgc tattgatctc tatccagaaa catatgctac taagattgtg gaattattta
     4201 aaggcactgc tgttgaagta gaagtctttg ataaaaaaga aattgaaaaa ttaggaatgc
     4261 atgcttttct tgctgttaat gccggaagtg ataaagaacc gcgttttgtg gtcatgaaat
     4321 attttggcaa taaaacgagt gataaacata ttacctttgt tggtaaaggc ttaacttatg
     4381 actcaggcgg ttacgatatt aaaccgggta aaagtatgtt tgaaatgttt actgatatgg
     4441 cgggttcagc ggcagttatt ggcgcaatta aagcaattgc cgataataac ttaaacgcaa
     4501 atgttgtcgc agttacagct ttaaccgaaa acttaattaa tggtaaagca tataaaaatg
     4561 gggacattat ttcaacgatg aaaggttcca ctgttgaaat cggttcaact gacgctgaag
     4621 gaagacttac acttgcggac gctatttatt acgccgccac taaattaaat tccacgcaca
     4681 ttattgaact atcaacatta actggtgctt gtgttgtagc gcttggtacg gatatgattg
     4741 gtgcgaccac taataatgaa gaattttaca ataaagtatt tgatgctggt aatcatgtcg
     4801 gcgaacctag ttggcgccta cctgttactg acgtcttaaa agaagcagta aaaggtgaat
     4861 ttgccgatct taaaaactct gttcctggtg gagcaggcac gattaccgct ggtatattcc
     4921 ttgaacactt cagtgaaaat attccatttg tccatttaga tattgcgggg acggcgtatg
     4981 gtaaagctaa aaggtattac gcgaacgggg caacaggagt gggcgttaga actttatata
     5041 aacttgtagc gacgaatttc tctaaataat aaattaatat tgcttattat acatactcca
     5101 aatcctagaa attaggaatg gggtattttc gaaataaaat atttaaggca aacatatatt
     5161 gattaactag tggtttagac agcaattaat ataagagatc gactcacaaa aattatttgt
     5221 tagtggtaat tagtaatata aaaatgtgtg gttttacgca gtggttttgt atttattatt
     5281 gaagatgaat taactatacg ctataattat aatataaaaa ctattgcgat atggaggatg
     5341 gttattttga atcttgaaag agcgaaagaa aagtttggtg taagagaacc attttacaaa
     5401 aatgaactta aagatttttt aaaaattgag aatcaaaata cttttaacca gctcctctct
     5461 aacatgaata aatttaatgt cgtaaagaaa atggagaatg ggatttatta ttttccttct
     5521 gctaatgaac actttaaaga acttaaacca tcagtaagca gtgtcgtaaa tttaaaatat
     5581 ttaaagaatt ataacggaat aagaactggt gcctttttag cgtataaata taaattaact
     5641 tcacaagtat caggatatta tgaaatcata acgaataatg taagtgaaca tacaagagct
     5701 aaaaaagaat atgatgggaa agtcattgtt tcgtcttcca agtttaatat tgataaaaat
     5761 aactatttgt acgctgaatt tttagagttg gtaaataata tgaattatag tgattatgat
     5821 tacaaaaaaa cactgaattt gataattgaa gtatttaaag agatgaattt agacatccaa
     5881 aaaacaaaaa aactagccaa aaattatcgc ggtagtgtgt atgcttcata tctccgaaaa
     5941 gtacaggaca ttttaaatga agttgcatga aaataaacat ttaaaagggg aatatttgat
     6001 tattcttact gtttaaatta aaacaactat cactaattaa tgattaatgt tattattaat
     6061 tatacactcc ttaaaacacc attattgaaa gggaaaaatt atgcaatgtg cagtaaaaag
     6121 atttgccgaa agcaaaaaca atggcctatg tttaattgat atgccaaccg gaactggtaa
     6181 aacttatcta accggaaaat taattgaaga ttatattaaa ggtaacatac tatcagatat
     6241 agatactatc atttatctta ccccattaaa gaaaaatgtt gatgatattt atgaagatgt
     6301 tcggaaagcg ttttctgatg acgctgaact ttttgataat aatgcgcttc gactatttcc
     6361 taattatgaa tgtgtcatag aaaatattac agaagaagta atactttcaa aaattcccca
     6421 gtctttgaag aaaaaggaat cttttaaaaa cccgtcttta gggttataca ctagaaaagt
     6481 ttaggggata aaaataataa ttaa
//
LOCUS       DAOPCD010000029         6379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_10727_length_6379_cov_4.726755, whole genome shotgun sequence.
ACCESSION   DAOPCD010000029 DAOPCD010000000
VERSION     DAOPCD010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10727_length_6379_cov_4.726755"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3012
                     /gene="nifJ"
                     /locus_tag="PLQ55_01520"
     CDS             <1..3012
                     /gene="nifJ"
                     /locus_tag="PLQ55_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPK28649.1"
                     /translation="GFRTSHEIQKVEVIDENEWKKLLPEDKVAEFRARALNPHANPVT
                     RGGAENDDIYFQGKEAQNTHYARVLDVACDYFAKVSKVTGRHYAPFVYLGAKDATKVI
                     VAMGSVTETIREVVADLNKKGEKVGLVKVHLYRPFSAKHLVDVLPETVKKIAVLDRTK
                     EVGALGEPLYLDVLTALKQQGRKVDVIVGGRYGISSRDTQPKHIKGVFDFLDNKPWHS
                     FTVGINDDVTKLSVPFDHDYHIDNGHTECLFYGLGSDGTVSANKSSVAIIGEHTDKYA
                     QAYFAYDSRKAGGPTRSHLRFGTNTIHSTYYVENASFISASQDSYLYKFDMIRYLKAG
                     GTFLLNTSLKGEELINALPNRVKYGLAMKNAKMYVIDANKIASEIGMGRHTNTILQSS
                     FFYLNQDIMPYEKALRLMKKYAEAAYGRRGKRIVELNYAAIDAGSHGIIEVKVDPEWK
                     KLKPLRTVELMGDKYFDEYVNPIDNLEGYDLPVSTFTKWDLLDGTMTNDITFKQKRSI
                     ATMVPRWVKENCIQCNTCSLVCPHATIRPFLLDEKEVAGLPENVREETVAAMGGPSVA
                     GLRFRIQVSPMNCVGCALCVVTCPGRGGNKALEMVEARSQFDIEPAADYLYKQVTYKT
                     DKFPLNSPKGVGFLMPYMEISGACAGCGETPYYRLITQLFGNDMLVANATGCSSIYCG
                     SVPGTPFTTNKDGEGVAWANSLFEDNAEYGFGMRMANDYKLKLIADIFTAALPKVEPE
                     LKELLEKYLANPRDRKEVRSYLPRLIELLEKSKDKGVKEALKYKDDILDKSVWIIGGD
                     GWAYDIGYGGLDHVLASGANVNVLVLDTEVYSNTGGQMSKATQTGAIAKFAASGKTGA
                     KKNLAMMAMAYGDVYVAQVALGANPVATIRALTEAENYDGPSIVIAYSPCTEHGIKGG
                     LSNHIAVEKKAVASGYWPIFRYDPRLEAAGKSPLTWDMKEPDYTQFREFVMGETRFSQ
                     LPTVNPENAEFLLSKSEEEAKKRYFRLKKFE"
     gene            complement(3224..4504)
                     /locus_tag="PLQ55_01525"
     CDS             complement(3224..4504)
                     /locus_tag="PLQ55_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28650.1"
                     /translation="MKNNKTKLFLLTALLMLVVSSCGESSLSSDNSISSSTYVSETTT
                     AIVSTSDEQSSVESTEDSAAESLNESSAEPSTSESSAEIPSASTAPLVETLVRTIDFE
                     ELDAGNMSATHHNTYYDGLIVADGRVTTTKKTSIDNKSLGMRMPASTTEEPRICTIKV
                     SGYTKIVLDLKQERTHTRIKTNTGAIYQGTKNEDILRDVEILTTELTELVFTFYTETL
                     ISSTMDCGFDNIRFYALVEEGGLPPSSETPTSSEPISSSEETSTTSEIPTSVDGTYDY
                     ITMRDIAEFLQTKDAHYPISDAFIKEYQQKTGGYIDYARQPGKVVDKSICEPDQKWHF
                     FDSWLYRTLEEGGITWTDSAKNRVYTKLLCPELLLWIYEACAAPEAKILAAYDAAVAG
                     KEAGTHISTIAKNMRACVPWEDLEINIQYFLDNK"
     gene            complement(4768..5616)
                     /locus_tag="PLQ55_01530"
     CDS             complement(4768..5616)
                     /locus_tag="PLQ55_01530"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HPK28651.1"
                     /translation="MRGRYKLKMLLEIAGLSHQTYAYNDKHMDEQANKDKLIEDEIVS
                     IFNENYQKYGIPRITLELKSRGHHINHKKVERLMKKLKIQARPKQRKYRSYRGEVGRI
                     ADNLLNQDFTTNDFYEKIGTDVTVFIGRFGKVYVSPIIDFHNREVLAYDASVNPDYRQ
                     INRMFQRLKIMHGNEFKNTIMHSDQGWQYQMKKYREKLAELKIIQSMSRKGNCLDNSP
                     TENFFSRMKEEMFYGKEHLYETEEQLIRAIDEYIYYYNNKRIVVRLKMSPIQFKNLQQ
                     GTLLCI"
     gene            complement(5631..6125)
                     /locus_tag="PLQ55_01535"
     CDS             complement(5631..6125)
                     /locus_tag="PLQ55_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28652.1"
                     /translation="MKHTISEKLQFVKMHIEENVPISEIATNYGFDPTSLKYCCSLYR
                     MYGEKAFEKKEKARTYTREEKLKAIAIILSGEKTIREVALGMMLSDPKIISDWVNKYR
                     REGEAGIKDTFSRAAYLKHDDRLFVKEHKKLLEDLERTKAENEYLKKSFPHILKRSKQ
                     PKKK"
     gene            complement(6190..6266)
                     /locus_tag="PLQ55_01540"
     tRNA            complement(6190..6266)
                     /locus_tag="PLQ55_01540"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6230..6232),aa:Pro,seq:ggg)
BASE COUNT         1918 a         1225 c         1209 g         2027 t
ORIGIN      
        1 ggttttagaa catcacatga aattcaaaaa gttgaagtca ttgatgaaaa tgaatggaag
       61 aaattattac cagaagataa agtagcggaa ttccgcgctc gtgcactgaa cccacacgca
      121 aatcctgtga cccgtggtgg tgcagaaaat gatgatattt atttccaagg taaagaagca
      181 caaaatacac attacgctcg tgtgcttgat gttgcttgtg attattttgc taaagttagt
      241 aaagtaaccg gtcgtcacta tgcaccgttt gtctatctcg gagccaaaga cgctacaaaa
      301 gtaatcgtcg cgatgggcag tgttactgaa acaatccgtg aagtcgttgc cgatttaaat
      361 aaaaaaggcg aaaaagttgg ccttgttaaa gtgcacttat atcgtccgtt tagtgccaaa
      421 catttagtcg atgttttacc agaaacagtg aagaaaatcg ctgtcttaga tcgcactaaa
      481 gaagtaggag cacttggtga accactttat cttgatgtgt taaccgcact taaacaacaa
      541 ggacggaaag ttgatgttat cgttggtggt cgctacggaa ttagtagccg tgacacacaa
      601 ccaaaacata ttaaaggagt atttgacttc ttagataata aaccgtggca ttcatttacc
      661 gttggaatta atgatgatgt cactaaactt agtgtcccat ttgatcacga ttatcatatt
      721 gataacggac atacggaatg cttattctac ggtttaggaa gtgacggcac tgtgagcgct
      781 aataagtcaa gcgtcgccat tattggtgaa cacactgata aatatgcgca agcttacttt
      841 gcctatgact cacgtaaagc tggtggtcca acccgcagtc acttacggtt tggcacaaat
      901 acgattcatt caacttatta cgtagaaaat gctagcttta ttagtgcttc acaagattct
      961 tatctttata aatttgatat gattcgctac ttaaaagcag gcggaacatt cttacttaac
     1021 acaagcttaa aaggtgaaga attaattaat gcattaccta acagagttaa atacggttta
     1081 gcaatgaaaa atgccaagat gtatgtaatt gacgcaaata agattgctag tgaaatcggt
     1141 atgggtcgcc atacaaacac aatcttacaa tcttcattct tctatctaaa tcaagatatt
     1201 atgccttacg aaaaagcgtt gcgcttaatg aaaaaatacg ctgaagcggc ttatggtcgt
     1261 cgcgggaaac gcattgtcga acttaactat gcggcaatcg atgctggctc acacggtatc
     1321 attgaagtta aagttgatcc agaatggaag aaacttaaac cactacgcac tgtggaactt
     1381 atgggtgata agtattttga tgaatatgtt aacccaattg ataacttaga aggttacgac
     1441 ttaccagttt caacattcac aaagtgggac ttacttgatg gcacgatgac taatgacatc
     1501 accttcaaac aaaaacgctc aatcgcgaca atggtgccac gctgggttaa agaaaactgt
     1561 atccaatgta atacatgtag tctagtgtgt ccgcacgcca caattcgtcc gttcttactt
     1621 gacgaaaaag aagtggcagg cttaccagaa aatgtgcgtg aagaaacagt cgcggcaatg
     1681 ggtggaccaa gcgtagcagg cttacgcttc cgtattcaag ttagtccaat gaactgtgtc
     1741 ggctgtgccc tttgtgttgt cacttgtcca ggacgcggcg ggaataaagc acttgaaatg
     1801 gttgaagcac gtagtcaatt tgacattgaa cctgctgccg actacttata taagcaagtt
     1861 acttataaaa ccgacaaatt cccacttaat tcaccaaaag gtgttggttt cttaatgcct
     1921 tatatggaaa ttagcggagc atgtgcgggc tgtggtgaaa caccttacta ccgcttaatt
     1981 acgcaattat tcggtaatga catgctagta gcaaatgcga ctggctgtag ctcgatttat
     2041 tgtggtagtg tcccaggtac accatttacc acaaacaaag acggtgaagg cgtcgcttgg
     2101 gcgaatagtc tgttcgaaga taacgcagaa tacggttttg gaatgcggat ggctaacgat
     2161 tataaactta aattaatcgc tgacatcttc actgcggctt taccaaaagt tgaaccagaa
     2221 ctaaaagaac tccttgaaaa atatctcgca aatccgcgtg accggaaaga agttagaagc
     2281 tacttaccac gcttaattga attacttgaa aaaagtaaag acaaaggcgt taaagaagca
     2341 cttaaatata aagatgacat tcttgataaa tcagtgtgga ttattggtgg agatggctgg
     2401 gcatatgata ttggttacgg cggtcttgat catgtccttg ctagtggcgc aaatgttaat
     2461 gtgcttgtgt tagacactga agtgtatagt aacaccggcg gacaaatgag taaagcaacg
     2521 caaactggtg ctattgctaa atttgccgca agtggtaaaa caggggcaaa gaaaaactta
     2581 gcaatgatgg cgatggctta tggggacgtt tatgtcgccc aagtcgcact tggcgctaac
     2641 ccagttgcca cgatccgtgc gcttactgaa gcggaaaatt acgacggacc atcaattgtt
     2701 atcgcttata gtccgtgtac tgagcacggg attaaaggcg gacttagtaa ccatatcgcc
     2761 gttgaaaaga aagccgtcgc tagtggttac tggccaatct tccgttatga cccacggctt
     2821 gaagcagcgg gtaaatcacc acttacttgg gatatgaaag aaccagatta cacacaattc
     2881 cgtgaatttg tgatgggtga aacacggttt agtcaactcc caactgtgaa cccagaaaac
     2941 gctgaattct tacttagtaa gagtgaagaa gaagcgaaaa aacgctactt ccgtcttaaa
     3001 aagtttgaat aacgctcttt aaattaaaag agagcctcgg gacgaccccg aggcttttca
     3061 ttttggacct cattttggac ctcattttta aatattatat acgacaatat atacgatatc
     3121 tgtataattt cgattataaa gtgtaacgat aacaccatta tcagaaatta aaaatattcg
     3181 tttttttact ttgtactcta tgtttaaaaa tataaataat tagctattta ttatctaaaa
     3241 aatattgaat attaatttct aagtcttccc acggtacaca agcgcgcata tttttagcaa
     3301 tagtggaaat atgagttccg gcttctttac ccgcaacagc agcgtcatag gctgctagta
     3361 tttttgcttc aggggcagca catgcttcat aaatccaaag taataattct ggacataata
     3421 gtttagtgta cacgcggttt tttgctgaat cagtccaagt tataccgcct tcttctagtg
     3481 tacgatagag ccatgaatca aagaagtgcc acttttgatc aggttcacag attgatttat
     3541 cgacgacttt accaggttga cgcgcgtaat ctatataacc accagttttt tgttgatatt
     3601 ctttgataaa cgcgtcagaa attgggtaat gagcgtcttt agtttgtaaa aattcagcaa
     3661 tatcacgcat tgtaatatag tcataagttc cgtctacact agtcgggatt tcgcttgtag
     3721 tactagtctc ttcactacta cttattggtt cagaagaagt aggtgtttca ctgctaggtg
     3781 gaaggccgcc ttcctccact agtgcgtaaa atcttatatt atcaaacccg cagtccatcg
     3841 tacttgagat taatgtttcc gtatagaaag taaacactag ctcagtaagt tctgtggtaa
     3901 gaatttcaac atcacgtaag atatcttcgt ttttagttcc ttgataaata gcgcctgtat
     3961 ttgttttaat gcgggtgtgg gttcgttctt gttttaaatc caaaacaatt ttagtgtagc
     4021 cgcttacctt aattgtgcaa atacgtggtt cttccgtggt agaagcaggc atccgcatcc
     4081 ctagtgattt attatcgata cttgtttttt tcgtcgttgt taccctgccg tcagccacaa
     4141 ttaaaccgtc gtaataagta ttatgatgtg tggcactcat attaccagca tctaattctt
     4201 caaaatcaat cgtgcgcact agtgtttcca caagtggagc ggtggacgca gaaggtattt
     4261 ctgctgaaga ttcagaagta gatggctctg ctgaagattc attaagtgat tcagcagcag
     4321 agtcttcagt tgattccact gaagattgtt cgtcacttgt cgacactatt gcagtagtgg
     4381 tctcactaac ataagtactt gatgaaatac tattatcact acttagtgaa ctttcaccac
     4441 aagaagatac cactaacatt aatagtgctg ttaataggaa tagtttagtt ttattatttt
     4501 tcatatacat taattctctt ctcttcaata atagcatttt tattaattct ttgtgttaat
     4561 cctaataact tactagaaat tctactggac agtttgacta aaaatggtaa aagtgttcag
     4621 tagcactgat caatatttat taatgttttt actactagtt aaaataaaaa aactcccgat
     4681 tgtaataatg tttggttcaa ttttagggag tgcttttaat atgagccttc ccccttattt
     4741 ttgcataaga gttgttaacc cctaccatta tatacacaac aaggtacctt gttgcaaatt
     4801 tttgaactgt attggactca tttttagtct tacaacaatt cttttattat tatagtaata
     4861 gatgtattca tcaatcgctc taattaattg ttcttctgtt tcatacaaat gttctttacc
     4921 ataaaacatt tcttccttca tccgactaaa gaaattttct gtcggagagt tatcaaggca
     4981 atttcctttt cgactcattg attggattat ttttaattca gctagtttct cgcggtactt
     5041 tttcatttga tattgccaac cttgatcact atgcataatc gtatttttaa actcattacc
     5101 atgcataatt ttaagccgtt ggaacatccg attaatctgg cgataatctg ggttcaccga
     5161 tgcatcataa gcaagaactt ctcggttgtg aaaatcaata atcggtgata cataaacctt
     5221 tccaaagcga ccaataaata ctgtgacatc agttccaatt ttttcgtaaa aatcgttagt
     5281 cgtgaagtct tgatttaaga gattatccgc gatacggcca acctcaccac ggtatgatcg
     5341 atacttcctt tgtttaggtc gagcttgaat ctttaatttt ttcatcaacc tctccacctt
     5401 tttatgattt atatgatgtc cacggctctt taactccaaa gttatccgcg gaatgccgta
     5461 tttttgatag ttttcattga aaattgagac tatttcatct tcaattaatt tatctttatt
     5521 tgcttgttca tccatatgtt tatcgttata tgcatatgtt tgatgtgata aacccgctat
     5581 ttcgagaagc atctttaatt tataacgccc ccgcattgct cgcacaaccg ctattttttc
     5641 tttggttgtt tgcttctctt gagtatgtgg ggaaatgatt tttttaagta ttcattctcc
     5701 gcttttgttc gctctaaatc ttccaataat ttcttgtgtt ctttcacgaa taaacggtca
     5761 tcatgcttta agtacgccgc ccgactaaat gtatctttaa tccccgcttc tccttcgcgc
     5821 cgatacttat taacccaatc agatattatc ttgggatcac tcagcatcat ccctaacgct
     5881 acttcgcgaa ttgtcttttc gccacttaaa ataattgcaa ttgctttgag cttttcttcc
     5941 cgtgtgtatg tcctggcttt ctctttcttt tcaaaagctt tctcaccata catgcgatac
     6001 aaactgcaac aatattttaa tgatgttgga tcaaatccat aattcgttgc aatctctgaa
     6061 ataggtacgt tttcttcaat gtgcattttc acaaattgaa gtttttccga gattgtgtgt
     6121 ttcatttaaa aattccccct ttttatttta ctctctttga gcaaaatttt gggggaagtt
     6181 tcaataaaat ggtcggaacg atgagattcg aactcacgac ctctaccacc ccaaggtagc
     6241 gcactaccaa gctgtgctac gtcccgacta caaatattat aaagtaattt aaggaaatta
     6301 gtagtgataa ctacatttgt ggcattttag cgttataatt atgttatgga aactaaaaac
     6361 ataaatacga aacaattat
//
LOCUS       DAOPCD010000030         6310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_10876_length_6310_cov_3.492566, whole genome shotgun sequence.
ACCESSION   DAOPCD010000030 DAOPCD010000000
VERSION     DAOPCD010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10876_length_6310_cov_3.492566"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLQ55_01545"
     CDS             complement(<1..738)
                     /locus_tag="PLQ55_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28653.1"
                     /translation="MAFGQFRAHLIAPRVIRVVLYSDKYQYEQTRVTIYDDNDKLVPF
                     SSKSKVVQYKVTAFELTLEQPFVLGQNYKVYLQNFGQVPLNVSEATTFPNFDTDYYYT
                     GPLGAEYTKEQTTFRLWAPLASRVILKYEVPGEKHFLYRKLKRGERGVYEYTLEGDHE
                     GIRYTYLVLNNGLEEEVTDPYALSSGPNGAFSYVINQNKYDDMNMHDDKLPPFTKPTE
                     AIIYETSVRDQTIDERTDIEYPATFYGM"
     gene            complement(744..1064)
                     /gene="acpS"
                     /locus_tag="PLQ55_01550"
     CDS             complement(744..1064)
                     /gene="acpS"
                     /locus_tag="PLQ55_01550"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPK28654.1"
                     /translation="MSIGIDIVYIPRFAGKEKLATRILSKNELEIYHMRPNKEEFLAG
                     RFAAKEAFLKAMKEGIGRVAFHKIDVVYKKDGRPEIHHDGNVYAVSISHDGDYAVAVV
                     QIKE"
     gene            complement(1067..3655)
                     /locus_tag="PLQ55_01555"
     CDS             complement(1067..3655)
                     /locus_tag="PLQ55_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPK28655.1"
                     /translation="MSEAKTNLLMQQQRLSRGLKGIIVALLALIGILKISIGGVINVP
                     VALLFGDLFVVFFALQVLFAFYLIFFPKAKSKKRVTLVFLSIFGLLIISSAFYAGNLR
                     NYMRDGTTFKDNLDVMVTLARDTFSFTPPSNAYFYNYGGILGEGLLFVLKDLPVWLII
                     IILVLANIGFATMLLWPLLVKIYAAIKEEIRDLKKRRRVLHEAGQLHKEEAPTEEDQV
                     PVTEIMNDNEVWGSNLLHRVGPSASEAEEKLGPPPALNVVKNENSGMHRAMPSEDFSA
                     FDEHFELPTLDTNEEEFEALDPTVFRTMEKVESEEDFQELERAVDEKGSFEAVSFTPH
                     HEEPTNTLNAEPKKDENVLREALEERYEPEPLPPLPEPVVKQENEQKPRISSNFANFR
                     LPKLDLLDDAIDNGEEAQNREFAAEYEAKINAFFTDFNVDARVFSWTIGSTFTRFEIQ
                     LGPTESVKAINPLLNDISIRLNGASVRFEEVLPGKATSGLEVANKFRTNVNFKDCMHK
                     IVANKRKKYLIPLGKDVAGNVILAPFTDFPHLIVAGATGSGKSVFIHTVLTSLIMMHS
                     PLELRLILIDPKRVELSAYNNIPHLITPIIKEPSEAKVALDRLIDEMENRYKLFEKTG
                     VGKLSDYNEIIEEMGGEKLPVIVAIVDEFADLFESEKEISNLVLRLVQKSRAAGIHLL
                     IATQRPSVQVITGNIKANIPSRVAFMTASVTDSMTILGCGGAENLLGYGDMLVDSITT
                     NYRGLLRVQAPYVQVREVLRVTNYLRNNYMTDFYPEFLDLKEKVNVYGGGGPVISDDL
                     YQAVLDFTLTQDTISISRLQQNFNLGWPRARQLYDQLMQNGIIEPPDEANSAKGAVVI
                     ANKRGN"
     gene            complement(3731..5419)
                     /locus_tag="PLQ55_01560"
     CDS             complement(3731..5419)
                     /locus_tag="PLQ55_01560"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPK28656.1"
                     /translation="MNDEVKIFALGGLDEHGKNMYVIEINNDIFVLEAGLKYPDKSSP
                     GIDFIIPNHEYLITNKSRIKAYFISHGHDDQFGALPFIYKQAPAPIYTTKAAAMMLER
                     FTRRVGVKDVKYEIFTIEPSSQHNIAGHKFTFFQTTHSAMHSFGIALDTSQGTVVYSG
                     DFIIEYTNSARYKLDLNSIAKIAEKNVLCLLSESSGAEKAGYTSPTHRLAPNIVPIFR
                     ETKGRIFISLYDQSTYNIEELVQVVINAKRKIAFYDPNTEEYFRDFAKAGLSLIDERY
                     IISNDDLLRARDQDIVILMLGAGEKIYEKIIALSNRENPDKKIVLGKDDTFIMALPAP
                     ATLEVIATEALDSLYTTGCNVVNITRKLFTNMHAQEEDLRMLLSLLKPKYYIPVKGLY
                     RQQIANAQLALHSGLKFSHHNIFLLDNGVSVEFNDGVGRVKMSPLDLIPSGDVLVDGT
                     GIGDINRGVIEDRQRLSEDGIIIMAIAVNYDERTITAGPDVQMRGFVYVKDSEQLLKQ
                     IRQIFINTVSEALDARQFDIDGAVKETESRVEKYIFRETRRHPMILPLIRVINE"
     gene            complement(5502..5849)
                     /locus_tag="PLQ55_01565"
     CDS             complement(5502..5849)
                     /locus_tag="PLQ55_01565"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HPK28657.1"
                     /translation="MSSDKKVTCLVCPRGCIMTVSSDLKVSGNFCARGVPYALQEVKE
                     PKRNLTYVVQVEGREAPLPVRTDSMIAKHLIPDVVKLLRTVTIKPPVAFNAIIIANVF
                     DTGVNIISSAEIK"
     gene            complement(5842..>6310)
                     /locus_tag="PLQ55_01570"
     CDS             complement(5842..>6310)
                     /locus_tag="PLQ55_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28658.1"
                     /translation="LSDNLGIPTSKSRGPVVNNNYQTQLPWFFVAGNALHIHDLADDA
                     YFEGEEAGRAASLFINGKLKAPTNTLQVVPGYGINYVIPNNLELPLNTEELVLKFRPK
                     ENYENKRIVVKIDDTVIFKKFFPFLFPSELVILKVKKELLVAGEKLIVEVSDE"
BASE COUNT         1911 a         1321 c         1078 g         2000 t
ORIGIN      
        1 catcccgtaa aatgtcgccg gatactcaat atcggtgcgt tcatcaattg tttgatcgcg
       61 cacacttgtt tcgtaaatta ttgcttcggt aggtttagta aatggcggta atttatcgtc
      121 atgcatattc atatcatcat atttattctg attaatgaca taactaaaag caccatttgg
      181 tccactgcta agggcgtaag ggtcagttac ttcctcttct aaaccgttat ttagcactaa
      241 gtaagtataa cgaattcctt catgatcacc ttcaagcgtg tattcataca ctccgcgttc
      301 accgcgttta agtttgcgat ataagaaatg tttttcacct ggtacttcat attttaaaat
      361 cacgcgactc gctagcggtg cccagagtct aaatgttgtt tgttctttag tgtactccgc
      421 accaagcggg ccagtgtaat aataatcagt gtcaaaatta ggaaaagttg ttgcctcact
      481 tacattaagc ggcacttgcc caaaattttg taaataaact ttataatttt gtcctaacac
      541 aaaaggttgt tctagtgtta attcaaaagc agtaacttta tattgtacga ctttactctt
      601 cgaactaaac ggcactaatt tatcgttatc atcataaatt gtcacacgtg tttgttcata
      661 ttgatattta tcactataga gcaccacgcg gattacccgt ggcgcaatta aatgtgctct
      721 aaattgtcca aatgccataa atactactcc ttaatctgta ctactgcgac cgcataatca
      781 ccgtcgtgac taattgacac agcataaaca ttaccgtcat gatgaatttc agggcgtccg
      841 tcttttttat acacaacatc aattttatga aaagcaacgc ggcctatccc ttctttcatt
      901 gcttttaaaa acgcctcttt agcggcaaaa cgccctgcaa gaaattcttc tttatttggt
      961 cgcatatgat atatttccaa ttcatttttc gataagatgc gtgtcgcaag tttttcttta
     1021 cccgcaaagc gcggaatata aacaatatca atcccaatcg acataattaa ttacctctct
     1081 tattggcaat aacaaccgcc cctttagcgc tattagcttc atcagggggt tcaataatgc
     1141 cattttgcat taattgatca taaagttgac gggcacgcgg ccatcctaaa ttaaagtttt
     1201 gttgtaatct gctgatagaa atcgtatctt gcgttaaagt aaagtcaagc actgcttgat
     1261 agaggtcatc actaataacg ggaccgccac caccatagac attaactttt tcttttaaat
     1321 ctaaaaattc cggataaaaa tcggtcatat agttattgcg taaataatta gtgacgcgga
     1381 gtacttctcg cacctgtaca taaggcgctt gtacacgtaa tagtccgcga taattagtcg
     1441 taatactatc aactaacata tccccgtagc caagtaagtt ttccgctcca ccgcaaccaa
     1501 gaatcgtcat actatcagtc acgcttgccg tcataaaggc aacacggcta ggaatattag
     1561 ccttaatatt accagtaata acttggacac ttggtctttg tgttgcaatt aataagtgaa
     1621 ttccagccgc gcggcttttt tgcacaagcc gtaatacgag gtttgatatt tctttttcac
     1681 tttcaaaaag atcggcaaat tcatcgacta tcgcgacgat aaccggtaat ttttcgccac
     1741 ccatctcttc gataatttcg ttataatcgc taagtttacc tacccccgtt ttttcaaaaa
     1801 gtttatagcg attttccatc tcatcaatga gccggtcaag cgctactttt gcttcacttg
     1861 gctctttaat aatcggtgtg attagatggg gaatattgtt gtaagcagaa agttcaacac
     1921 gttttgggtc aattaaaatt aatcgtaatt caagtggtga atgcatcata ataagggaag
     1981 ttaacaccgt atgaataaac acacttttcc cgctgcctgt tgccccggca acaataagat
     2041 gcgggaaatc agtaaacggc gctaaaataa cattaccagc gacatcttta ccaagcggaa
     2101 ttaaatattt cttacgctta ttcgcgacaa ttttatgcat acaatcttta aaattaacat
     2161 tcgttctaaa tttgttcgct acctctaagc cacttgttgc tttacctggt aaaacttctt
     2221 caaaacgcac agaagcacca tttagtcgta ttgaaatatc atttaaaagc ggattaattg
     2281 ctttaacact ttctgtcgga ccaagctgaa tttcaaagcg ggtaaatgtt gagccaatcg
     2341 tccagctaaa cacccgcgca tcaacattaa agtccgtaaa gaacgcatta atcttagctt
     2401 catattccgc agcaaattcg cgattttgtg cttcttcacc attatcaata gcgtcatcaa
     2461 gcaaatcaag tttaggtaat ctaaagtttg cgaaattcga agaaatacgc ggcttttgtt
     2521 cattctcttg cttcacgact ggttcaggaa gtggtgggag cggttcaggt tcatagcgct
     2581 cttctagagc ttcgcgtaaa acattttcgt cctttttagg ttcagcgtta agtgtattag
     2641 taggttcttc atgatgcggg gtaaaactaa cagcttcaaa actccctttt tcatcgacag
     2701 cccgttcaag ttcttggaaa tcttcttcac tttcgacttt ttccatcgtg cgaaaaacgg
     2761 tcggatcaag tgcttcaaat tcttcttcat tagtgtcaag tgttggtaat tcaaaatgtt
     2821 catcaaaagc ggaaaaatct tcgctaggca tcgcgcggtg catcccgcta ttttcatttt
     2881 ttacaacatt aagtgctggc ggtggcccta atttttcttc agcttcacta gcgctaggac
     2941 caacacgatg caataaatta ctgccccata cttcgttatc gttcattatt tccgtaacgg
     3001 ggacttggtc ttcttctgtg ggcgcttctt ctttatgtaa ttgaccagct tcgtggagca
     3061 ctcgtcttct tttctttaag tcacgaattt cttctttaat ggctgcatat attttcacaa
     3121 gcagtggcca gagtaacatt gtcgcaaaac cgatatttgc taacactaaa ataataataa
     3181 tgagccacac tggtaaatct ttaagtacaa ataaaaggcc ttcacctaaa ataccgccat
     3241 aattataaaa gtaagcattt gacggtggcg taaaagaaaa tgtatcacgg gcaagcgtta
     3301 ccattacatc taaattatct ttaaaggttg tcccgtcacg catataattg cgtaaattac
     3361 cagcgtaaaa ggcgctacta ataattaata agccaaaaat actaagaaac actagggtaa
     3421 ctctcttttt acttttagct tttgggaaaa agattaaata aaaagcaaat aatacttgta
     3481 aagcaaaaaa gacgacaaat aagtcgccaa ataaaagtgc aaccgggaca ttaatgacgc
     3541 caccaatcga aatttttaaa atcccaataa gtgccaaaag cgcgacaata atgcctttta
     3601 agccacggct aagtcgctgt tgttgcataa gtaaatttgt ttttgcttca ctcatactta
     3661 atattataca taatattact tgaatactcg aaaaagagag caaaaattaa taaatttttg
     3721 tgattaattg ttattcgtta attacccgaa tcagcggtaa gatcatcggg tgccgtcttg
     3781 tttcacggaa aatatacttt tcaacacgtg attcagtttc ttttaccgcg ccatcaatat
     3841 caaattggcg cgcgtctagt gcttcactta cagtattaat aaagatttgc cgaatttgtt
     3901 ttaatagttg ttcactatct ttaacataca caaagccacg catctgcaca tcagggcctg
     3961 cggtaattgt gcgttcatca taattaacgg caattgccat aataataatt ccgtcttcac
     4021 ttaagcgttg gcggtcttca atgaccccgc gattaatatc accgattcct gtcccgtcca
     4081 ctaacacatc accactagga ataaggtcaa gtggactcat cttcacgcgc ccgacgccat
     4141 cattaaattc aacactgact ccgttatcta gtaagaatat attatgatga ctaaacttta
     4201 agccactatg cagcgcgagt tgagcattag cgatttgttg ccggtataaa cctttaacag
     4261 gaatataata ttttggtttt aaaagtgaga gtaacatccg taagtcttct tcttgcgcat
     4321 gcatattcgt aaataatttg cgtgtaatat tgacgacgtt acacccagtt gtgtataagg
     4381 agtcaagtgc ttcggtggca ataacttcaa gtgtcgctgg agcaggcaaa gccataataa
     4441 aggtgtcatc tttgccaagt acaatctttt tatcaggatt ttccctattg gaaagggcaa
     4501 taatcttttc atagattttt tcaccagcgc caagcattaa aataactata tcttggtctc
     4561 tagcgcgtaa taagtcatca ttactaataa tataacgctc atcaattaag cttaaaccag
     4621 cttttgcaaa atctctaaag tattcttcag tatttggatc gtaaaaagca attttccgtt
     4681 tagcgttaat cacaacttgc actaattctt caatgttgta tgttgattga tcataaagcg
     4741 aaataaagat acgccccttt gtttcacgaa aaattggtac aatgtttggt gctaggcgat
     4801 gtgtaggcga agtatatccc gccttttcgg caccactaga ttcactaagt aaacataaca
     4861 catttttctc ggcaattttg gcaatgctat taagatcaag tttatagcgt gcactgttcg
     4921 tatactcaat aataaagtca ccagaataca caaccgtgcc ttgactagtg tctagcgcaa
     4981 tcccaaagct atgcatcgcc gagtgtgttg tttggaaaaa agtaaactta tgaccagcaa
     5041 tattatgctg acttgatggt tcaatcgtaa atatttcata cttaacatct ttaacaccaa
     5101 cacgacgagt gaagcgttca agcatcatcg ccgcggcttt tgtcgtgtaa atcggtgcgg
     5161 gtgcttgttt atagataaac ggtaatgcac caaattgatc atcgtggccg tgactaataa
     5221 aataagcttt aatccgactt ttattcgtaa ttaagtattc gtgattcgga ataataaaat
     5281 caataccagg tgaagattta tctggatact ttaacccagc ttctaacaca aaaatatcgt
     5341 tattaatttc aataacgtac atatttttgc cgtgttcatc tagcccccct aatgcgaaaa
     5401 tttttacttc atcgttcata attatacctc ttgttaataa tatgtgatat ctctactttg
     5461 ttagtataac aaaagtttag ctaaactacc aagtaattac tttatttaat ttcggcacta
     5521 ctaatgatat taacaccagt atcaaagaca ttggcaataa taatagcgtt aaacgcaact
     5581 ggtggcttaa tcgtaacagt gcgtaataac ttgacaacat cgggaattaa gtgctttgca
     5641 atcatcgagt cagtacggac gggtaaaggt gcttcacgcc cttcaacctg cacaacataa
     5701 gttaaattgc gtttaggttc ttttacttct tggagcgcat aagggacacc acgcgcacaa
     5761 aagttaccgc ttacttttaa gtcgctgctc acagtcataa tacaaccgcg cggacaaaca
     5821 agacaagtta cttttttgtc actactcatc acttacctcc acgatgagtt tttcaccagc
     5881 gactaatagt tcttttttta cttttaaaat cactaattcg ctcggaaata aaaacgggaa
     5941 gaatttctta aatatcaccg tatcatcaat ctttacaaca atccttttat tttcataatt
     6001 ttcttttggt ctaaatttaa gcactaattc ttcagtgttt aaggggagtt ccaaattatt
     6061 aggaataaca taattgatgc cgtagcctgg cactacttgt agcgtgttag tgggcgcttt
     6121 taactttccg ttaataaata aactagccgc tcttccagct tcttcacctt caaaataagc
     6181 atcatccgct aagtcatgga tatggagtgc gttacccgct acaaaaaacc atggtaactg
     6241 tgtttgataa ttgttattta ctactggccc ccgtgattta ctcgtgggaa tcccaagatt
     6301 atcactaagc
//
LOCUS       DAOPCD010000031         5923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_11714_length_5923_cov_3.997614, whole genome shotgun sequence.
ACCESSION   DAOPCD010000031 DAOPCD010000000
VERSION     DAOPCD010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11714_length_5923_cov_3.997614"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PLQ55_01575"
     CDS             <1..562
                     /locus_tag="PLQ55_01575"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HPK28659.1"
                     /translation="VINTPFEDSLYHFYLNYKIGNLRASGTFSMDDLAFTFDPKVDFG
                     LLDWGRGVWPFHEVWYWGQVNDYLADGRMINLNLGYGFGNLEAASENMMIIDGKAYKL
                     GRLYIENDVTLDYMADWIIKSDDGKIDLVMKTIYDRYSDNDFKVIFMKCHQVYGRYYG
                     TITLDDGTVVELTGQQGFFERSHNKW"
     gene            complement(661..1167)
                     /locus_tag="PLQ55_01580"
     CDS             complement(661..1167)
                     /locus_tag="PLQ55_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28660.1"
                     /translation="MKTLKLRVLNPRMHNVIYMFDGKALKPKGDNMGHYVFNIETPAD
                     KVDILIIRRSPLRSRLWLVWQFLFFIVSLLGILDLQSKKLNKEAIYRATLYLSGEDEV
                     DLKFDTDNSSNAFVELTTTLQVEERENKTLSDPLIVRRAKVLKILKIITYIVLLITLI
                     IILILIKK"
     gene            complement(1164..1670)
                     /locus_tag="PLQ55_01585"
     CDS             complement(1164..1670)
                     /locus_tag="PLQ55_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28661.1"
                     /translation="MQDTFNYNDYQNDQIVKKYDGRINRHHLGILFWRLGVAFSIIFI
                     VLLLARTLISITSIIIFMIGLVILSFIFLITLGTILIIDKNHGRHWKNLINILENRET
                     ALLVIDKILTFVPLICSVIIAFNIFALILLYTSKLPVRRFRLVTAIIIIILNIVALIY
                     ALAGGNFT"
     gene            complement(1816..2820)
                     /locus_tag="PLQ55_01590"
     CDS             complement(1816..2820)
                     /locus_tag="PLQ55_01590"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPK28662.1"
                     /translation="MKFERTLAICLDMNGCPNRCLHCWLGDLPNHKLNDKTATKIVKL
                     FKPYFENITFYSWLREPDFTKDYKARWLKDNKLSTIKPKRFELASYFTIVRDPEYVKW
                     LKAVGTKKVQLTFFGLEAMTDKYVGRKGAYKELIAATKILQDNDIDVRWQLFINEENK
                     DEVSHLLDVAAENKVTDIFVHEGSCDGNNRKLYDIRINKDNIPLKVKPYYLNYENLLS
                     ESECIELLKDDDSYFIPKYKDEIVIYITSDLNVYFNFTNPSAAWQIGNIEDDDISEIV
                     RKIVAGDVPALKFARSILLSELVKLYGDSNSDKVFSLEDYKMYLLNNYVDDKYNSKLQ
                     "
     gene            complement(2994..4010)
                     /locus_tag="PLQ55_01595"
     CDS             complement(2994..4010)
                     /locus_tag="PLQ55_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28663.1"
                     /translation="MRKSKYKPLADYLSASNKNSVSLTFNQIEKILGFKLPPSSRKYR
                     ANWSNNKMEALAWGWLPVGYESFDVRMSDEIANFRKVGNKLKSEKSKKNNRKQNSDLK
                     RQIENATDLIFLDVPNNLLKLEVDEYYMVKLTKTNSEIVEHELANDISYPQKAKKICE
                     ELIKRNNDFSSETFNIIIHRIAIENSTRSSKKTIDLLTDFALNSENQFLQRVKKGDLT
                     LVDDITDYLFKNNNRRDKSLASKVCRYLNEWLFDKDDFTINDSVVRKVLPYYLAYYKI
                     EKHYWANKNLDKLTYVEFFAIFEKIKEKLPELTRHELDHLLWYSYKNDNIRSTIAASL
                     AKHL"
     gene            complement(4288..4650)
                     /locus_tag="PLQ55_01600"
     CDS             complement(4288..4650)
                     /locus_tag="PLQ55_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28664.1"
                     /translation="MEFKTVNYDSKIIKEGISHKGRRKSDDPVKIFSQQLKDQNVLYF
                     FYKDDECLYIGQTGICLWDRIIRHEDPEKDSKWFEEANKICLIILDKKVDVISRRNLE
                     STFIVNYLRAGHKLYNKE"
     gene            complement(5093..5167)
                     /locus_tag="PLQ55_01605"
     tRNA            complement(5093..5167)
                     /locus_tag="PLQ55_01605"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5133..5135),aa:Met,seq:cat)
     gene            complement(5207..5881)
                     /locus_tag="PLQ55_01610"
     CDS             complement(5207..5881)
                     /locus_tag="PLQ55_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPK28665.1"
                     /translation="MPNYNELKGNNLNFSIEKNKPNILKGKTLLFLGASFTYGHASFG
                     ESFVEYIEVRNDCTCIKEAVSGTTLVEHIEDSYITRLKKVPLGKKYDALLCQLSSNDV
                     RLKQEFGVIKESDYDTKTICGAIQYIAKYARDVLKCPVIFYTCPYFDKERYQKLVSVL
                     NEIATKMQFSVIDMYNDKNFNNISAETYALYMADPVHPTKAGYYFWLTPYIESHLFAF
                     FTKLIR"
BASE COUNT         1921 a          980 c          860 g         2162 t
ORIGIN      
        1 ggttattaat acaccatttg aagattcttt gtatcatttc tatctcaatt ataaaattgg
       61 taatttacgc gcaagtggaa cgtttagcat ggatgactta gcgtttactt ttgacccgaa
      121 agttgacttt ggcttacttg actggggtcg tggggtgtgg ccattccatg aagtatggta
      181 ctggggtcaa gtaaatgact acctcgcaga tggacgaatg attaatctaa acttaggcta
      241 cggttttggt aatttagagg ccgctagtga aaatatgatg attattgatg gtaaagctta
      301 taagcttggt cgcctctata ttgaaaacga tgtaacgctc gattacatgg ctgactggat
      361 tattaagtcc gatgatggta agatagattt ggtcatgaaa actatttatg accgctatag
      421 tgataacgac ttcaaagtta tctttatgaa atgccaccaa gtttatggtc gctactacgg
      481 tacaattaca ttagatgacg gtactgtggt tgaattaact ggccaacaag gcttcttcga
      541 gcgctcccat aacaagtggt aatttaatta gtgtttcctg ccataagtgc tgctcaataa
      601 gtcctgccta aatgaaaagt cacattgcaa aatgtggctt tttatttttg gatatttaaa
      661 ttatttttta attaaaatta agatgataat tagagtaatt aaaagcacaa tatatgtgat
      721 tatttttaga atttttagca ctttagcgcg ccgcacaata agtgggtcgc taagtgtttt
      781 attttctctt tcttcaactt gtaaggttgt tgttagttcc acaaaagcgt tactcgaatt
      841 atcagtgtca aattttaagt caacttcatc ttcaccactt aaataaagtg tggctctata
      901 tattgcttct ttattaagtt ttttactctg taaatccaaa ataccaagga gtgaaacaat
      961 aaagaacaaa aattgccaca ctaaccataa tctgctgcga agtggagagc ggcgaataat
     1021 aaggatatcg actttatccg ctggggtttc aatattaaaa acatagtggc ccatattatc
     1081 accttttggc tttagcgctt taccgtcaaa catataaata acattatgca tacgcgggtt
     1141 aagcactctt aactttaaag tcttcatgta aagtttcctc ccgctaaggc ataaattaat
     1201 gcgacgatat ttaaaataat aatgataata gcagttacta aacgaaagcg acgcactggt
     1261 aacttactag tgtagagtaa gataagcgca aagatattaa aagcaattat tacactacat
     1321 attagcggaa caaaagttag tattttatca attactaaaa gcgcagtctc cctgttttcg
     1381 agtatattaa ttaagttttt ccagtgccga ccatgatttt tatcaataat taaaatcgtt
     1441 ccaagcgtaa ttaaaaagat aaaagaaaga atgacaagcc cgatcataaa aatgataatg
     1501 ctagtaatcg aaatgagtgt tcttgctaag agtaaaacaa taaagataat actaaaagca
     1561 acacctaacc gccaaaataa tatacctaag tgatgacgat taatacggcc gtcatatttt
     1621 tttactattt gatcgttttg ataatcgttg taattaaaag tgtcttgcaa tttcctttct
     1681 ccttgctttt aaatgaagta tttttctaat tgtatcttaa atttagactt tatcctaaaa
     1741 atacctaatt gtaattaaat agtctaacta atgttttaag tctaaaaaac tggcaaaaat
     1801 gcactatttt gctctttatt gtaatttact attatattta tcatcaacat aattattgag
     1861 taaatacatt ttataatctt caagtgaaaa gactttatcg gagtttgaat caccgtataa
     1921 ttttacaagt tcactaagca aaatacttct ggcaaatttt aaagccggaa catcacctgc
     1981 gacaattttc cgcacaattt cactaatgtc gtcatcttca atatttccga tttgccaagc
     2041 agcactagga ttagtgaaat taaaataaac atttaaatca ctcgttatat aaatgacaat
     2101 ttcatctttg tatttcggga taaaatatga atcgtcatcc tttaaaagtt caatacattc
     2161 actctcagac agaagatttt cataatttaa gtaataaggt ttaactttaa gaggaatatt
     2221 gtctttatta atacgaatat cataaagttt acgattatta ccgtcacaac ttccttcatg
     2281 cacaaatatg tcagtgacct tattttctgc cgctacatct aataaatgtg acacttcatc
     2341 cttattttct tcattgataa ataattgcca tcgtacatcg atatcgttat cctgcaagat
     2401 tttagttgca gcgattaatt ctttatatgc tcccttacgt cctacatact tatcagtcat
     2461 agcttctaaa ccaaaaaaag ttaattgcac ttttttcgta cccactgctt ttaaccattt
     2521 aacatattca ggatctctga caattgtaaa gtagcttgcc agttcaaagc gttttggttt
     2581 aatagttgaa agtttattat cttttaacca tctagcttta taatctttgg tgaaatctgg
     2641 ttcccttaac caactataaa aagtaatatt ttcaaaatat ggtttaaaaa gttttacaat
     2701 tttcgtcgcc gttttatcat taagcttatg attaggtaag tcacctaacc agcaatgtag
     2761 gcaacgattt gggcagccgt tcatatcaag acaaattgct agtgttcttt caaatttcat
     2821 ctttaaatcc tcatttatat aatttataac gctgattacg acttgttctt ttaagcggat
     2881 attcacgaat cactaaattg tattttaacg ctggatcaag ataattttta cgcagtgttt
     2941 ctttactttt tagattgaat aatatttgta tctgacttgc tttgtattag aaattataaa
     3001 tgtttagcaa gcgaagcagc aattgttgag cgtatattat catttttata agaataccaa
     3061 agcaggtgat ccaattcatg gcgggttagt tcaggtaact tttcttttat tttttcaaaa
     3121 atagcaaaaa attcaacata ggttaattta tcaagattct tattagccca atagtgcttt
     3181 tcgattttat agtaggctaa atagtacggt agaaccttgc gtacaacaga atcattgata
     3241 gtgaagtcat ctttatcgaa taaccattca tttagataac ggcaaacttt acttgctaaa
     3301 ctcttatctc ttctattatt atttttaaat agataatcag tgatatcatc tactaaagtt
     3361 aaatcaccct tttttactct ttgtaaaaat tgattttcag aattaagtgc gaaatcagtt
     3421 aataaatcta tcgttttttt ggagctcctg gttgaatttt caatcgctat tctatgaata
     3481 ataatgttaa atgtttcgct ggaaaaatca ttatttcgct tgattagttc ttcacatatt
     3541 ttcttagctt tttgaggata agaaatgtca ttagctaatt catgctcaac aatctccgaa
     3601 tttgtttttg ttagttttac catgtaatat tcatcaactt ctaatttaag taaattattt
     3661 ggaacatcta gaaatatgag atctgttgca ttttcaattt gtcgttttag gtctgaattc
     3721 tgcttgcgat tattcttttt acttttttcc gattttaatt tattaccaac tttcctaaag
     3781 tttgcaattt cgtcactcat tctcacatca aaggattcat atcctactgg aagccaaccc
     3841 catgccaatg cctccatttt attatttgac cagttagcac gatattttcg agatgacggc
     3901 ggcaatttaa aacctaggat tttttctatt tgattaaatg ttaaacttac agagttttta
     3961 ttagatgctg acaaataatc tgccaatggt ttatatttcg attttcgcat ataacatcac
     4021 ttctctttgt ccatattata taacaaaaaa ttaaggctta ttttatactg agtttttttc
     4081 atttgttaac tacttttaaa tttatgctct ttattttctg cttttacaac ttgagattat
     4141 tatttattaa aagacataat ctaatcttga gaacctataa aaattacatt taaacattta
     4201 taaatggcaa cattttgtaa ttggcttgaa taaactcttt ttatacaata gctaatactt
     4261 ttatattcaa aagtcttaaa aatgaaatta ctctttatta tataatttgt ggccagctcg
     4321 taaataatta actatgaatg ttgattctaa gtttcttcta gatataacat caactttttt
     4381 atcaaggata attaaacata ttttatttgc ttcttcaaac cattttgaat ctttttctgg
     4441 gtcctcatgg cgaataatcc tatcccaaag acaaatgcca gtctgaccaa tatacaagca
     4501 ttcatcatct ttgtagaaga aatataaaac attttgatct tttaattgtt gagaaaatat
     4561 cttcacaggg tcatcacttt tgcgtcttcc tttatgagaa atcccttctt ttattatttt
     4621 tgaatcataa ttaacagttt taaattccat aattcctcct ttagattatc tctcaaatta
     4681 gattgttcct ttttctgtaa agcgatgatt taagtatgaa gtcgaacaac catgccttta
     4741 caaggtttta tcgatttaca attaaagaaa tttcatgttc gtctacttca aaatcaagaa
     4801 accgattgta aagccgtaat ccgtgaaaat gttcctttga atttttttca tatgttttaa
     4861 aaattttatc atatatattt tcaattttgg gcttatttca aaaggttttt gactttcttt
     4921 tttaagaaaa agcactaaat tttatttaat aggcgaacaa gctaacataa taaacatctc
     4981 gtatttcttg ctcagagtta aaacttttaa tatgttaagg caaaaatatg tgttttgtac
     5041 gaaaaaaggt ttgatacatt gtttctattg tatcaaacct ttgctgttaa cttggttgcg
     5101 gagggaggat ttgaacctcc gacctccgga ttatgggccc gacgagctac cactgctcta
     5161 ctccgcgatg tattagtatt ataacatgat tattaattaa tgcaaattat ctaattaatt
     5221 tagtgaaaaa ggcaaataag tggctttcta tgtaaggagt aagccaaaaa taatatcccg
     5281 ctttagttgg atgaacagga tcagccatat ataaagcgta tgtctccgcg gaaatattat
     5341 tgaagttttt gtcattatac atatcaatga cactaaactg cattttcgta gcaatttcgt
     5401 taagtacact tactaatttt tgataccgct ccttatcaaa ataaggacaa gtgtaaaaaa
     5461 taacaggaca cttaagcaca tcacgagcgt actttgcaat atattgaata gcgccacata
     5521 ttgttttcgt atcgtaatca ctttctttaa taacaccaaa ttcttgttta agccgcacat
     5581 cattacttga gagctgacaa agtaatgcgt catacttctt gcctaacggt acttttttta
     5641 gccgagtaat ataactatct tcaatatgct ccacaagtgt tgtgccgctc acggcttctt
     5701 taatacaagt acaatcattc cgcacttcaa tatattcgac aaacgattca ccaaacgaag
     5761 cgtgaccata agtaaaagac gcaccaagaa ataataatgt tttacctttt aaaatatttg
     5821 gcttattttt ctcaatacta aaatttaaat tattgccttt aagttcatta taatttggca
     5881 tatttaactt tttaaccgtt cagtaatagc ttgcgcttta cga
//
LOCUS       DAOPCD010000032         5820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_11951_length_5820_cov_3.730095, whole genome shotgun sequence.
ACCESSION   DAOPCD010000032 DAOPCD010000000
VERSION     DAOPCD010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11951_length_5820_cov_3.730095"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /gene="glyA"
                     /locus_tag="PLQ55_01615"
     CDS             complement(<1..570)
                     /gene="glyA"
                     /locus_tag="PLQ55_01615"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173571.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPK28666.1"
                     /translation="MEKLFELVALETKRQNEGLELIASENFTSKKVRALAGSVLTNKY
                     AEGYPGRRYYGGCHYIDEIEGTAIELAKKLFNAKYANVQPHSGSSANMIAYASVLELG
                     DSILTLSLDEGGHLTHGSPVNFSSKHYKVTHYGLKDDGTIDYEALREIALKLKPKLIL
                     AGFSAYPFIIDFARIGKIAKEVGAYFMVDM"
     gene            667..1404
                     /locus_tag="PLQ55_01620"
     CDS             667..1404
                     /locus_tag="PLQ55_01620"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPK28667.1"
                     /translation="MRIKEHPIFKVPLAHRGLHNETIDENSMPAFEAAVKAGFGIELD
                     VHITADNKIVVIHDNTTTRTTGVEKVVNDTTYKDLKKLRLLHTKTEIPLLKDVLKMVK
                     GKVPVLVEVKAENKVPPALVTEVLKVIEKYPYKDKLAMQSFNPYVARDLKKGSTGIPV
                     GQLMSDVLYGQSKLVHFMYRSLLVLKISKPDFFNYEVKYIHKRNIQRKRKRLPLITWT
                     IDDYDKEKTAIALADNYIFEHIEIKER"
     gene            1406..2890
                     /locus_tag="PLQ55_01625"
     CDS             1406..2890
                     /locus_tag="PLQ55_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK28668.1"
                     /translation="MELKLNMKRTFLVGLAFMSIMLFWTMYDTLVAKMLVDNFGLNQT
                     FSGFIMALDNILALFLLPLFGRISDKTRTKLGKRTPFIIIGTLAASILFVSIAFFDLY
                     QQIAVTKHGFSLIESIGTAENPLYQFTLGTEVYTNNVRSALATERAQLIFQQVTKQQP
                     GLLIGFIVVLFFVLVAMGIYRSPAVSLMPDVTPKPLRSKANAVINFMGTMGGAFAYVL
                     VFLLASVPIFDKTGYVATFATTSLLMLLLIAIFVATVKENAWYKEMVEITEKYHLDSL
                     DIDDEGLDVSQHIDDEKMPKDVRRSFILILSAVVLWFMGYNAATTKFSVYAFNHLGLE
                     QFSLPPLIGIITAALAFIPIGYISQKIGRRRMILFGIVVLTIALGSAVFITGETKVLM
                     YIIMGLVGISWASINVNSYPMVVEMSRHGNIGVYTGYYYSASMSAMIITPILSGALMD
                     ITGWYPVLFIYSAVFMGLAFIPMYFVKHGEPAPVVLKEKAQKDE"
     gene            3056..3340
                     /locus_tag="PLQ55_01630"
     CDS             3056..3340
                     /locus_tag="PLQ55_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28669.1"
                     /translation="MKKKKTMVRLWGDHIVISSLFIGLAISLAFVALTFVTIEVLAKH
                     VAIIAEKKKDFIYGFGTLAILVAFVINNFWIKPQRNIVKSSDLNKKEREG"
     gene            3344..4735
                     /locus_tag="PLQ55_01635"
     CDS             3344..4735
                     /locus_tag="PLQ55_01635"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HPK28670.1"
                     /translation="MVITFSLILQMVGIALLMTALYLIIGIIPGTDETSVLVPMTLIL
                     VTLKLNPLVIMAGFIAAVVTLNIADSIPTAITSIPGGVMSTPLVGHSQTLKKHGLTSY
                     SVQWMMLGSLIGVLVSFPIALAFWGIFEFISVQTGLEISNFIKRYNEYIFLGGAILMS
                     LTSKEKVVSLLSIVPFGFATAFLRNPGLIINNTLLNTLIGGRTLSMTPFFLSITTGPL
                     VFALFELFFPSVFKNQKVEGKMTVILDDDVKKTKHGHKKIIAKTGIASALGSLLFFMS
                     PVGVTMMLGEFATKDEKDEVQQALQGVSAVNGLSSATYLAGIMISLFVFAFPISPAAL
                     GPGSVIFSPGGPIILEGNAGLAVPKGYLLIAIVIGLVIGLSLTVWLGMKYSHKMTELV
                     FKYISQETVLIFLFSIIALLSFAEAGLYGLIIVVSVGLLSGWLNKKGAGYGVQFMALY
                     AGGLFITILKALF"
     gene            4749..5639
                     /gene="mvk"
                     /locus_tag="PLQ55_01640"
     CDS             4749..5639
                     /gene="mvk"
                     /locus_tag="PLQ55_01640"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HPK28671.1"
                     /translation="MDGYGLAKIILIGEHSVVYGHPAIAIPFYAMRSEVVLTPHEEFI
                     LDTKYYTGPLNNAVDELHGLVALITHMAEIYKKIKPVIISVRSSLPEKSGLGSSASVA
                     KAVIDAFNNHFHLELTHIDYFELLKISENIYHHNASGIDASTVIYEKPIIYQRPHIKP
                     LKFNIDGYLAVFYSNTNSATKSAVAHVSTHPERDIHIDALGSLTANAKWALENNDITL
                     LATMFNAAHTHLRALGISTPTLEELRTKLLDAGALGVKITGGGMGGCLIALFNDEEKA
                     LATKEKLKSFPSWMIDLRNM"
     gene            5639..>5820
                     /locus_tag="PLQ55_01645"
     CDS             5639..>5820
                     /locus_tag="PLQ55_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HPK28672.1"
                     /translation="MKYKKANVNIAILKYWGKVNALINIPYQTSISVTADNFYTLTNV
                     ELDPKATKDIVILDGTA"
BASE COUNT         1749 a         1029 c         1086 g         1956 t
ORIGIN      
        1 catatcaacc ataaaatacg ccccaacttc tttagcaatt ttaccaatac gcgcaaaatc
       61 aataataaaa ggataagcag aaaatcccgc aagaataagt tttggtttaa gttttaaagc
      121 aatttcgcgt aatgcttcgt aatcaattgt gccgtcatcc tttaaaccgt aatgcgtcac
      181 tttataatgt tttgatgaga agttaacagg gctcccgtgt gttaagtgcc cgccttcatc
      241 aagtgagagg gttaagatgc tatcacctaa ctctaataca ctagcgtaag caatcatatt
      301 tgcactacta ccactgtgtg gttgtacatt tgcgtactta gcgttaaata gtttttttgc
      361 taattcaata gcggtgcctt caatttcatc aatataatga catccgccgt aatacctacg
      421 ccctggataa ccttcagcgt acttatttgt taatactgat cccgctaatg cacgaacttt
      481 tttacttgta aagttttcac tagcgattaa ttccaatccc tcattttgtc tttttgtttc
      541 tagcgctact aattcaaata atttttccat aacttagacc atccttacag tattagatta
      601 tagactaatc tagtctaaaa agtgctgtta attgactttt tatggtattt tcgtgaaaat
      661 aaatacatgc gtattaaaga acatccaata ttcaaagtcc cacttgcaca tcgcggactt
      721 cataatgaga caattgacga aaactcgatg cctgcttttg aggcggcggt aaaagctggt
      781 tttggcattg aacttgatgt gcatattacc gctgataata agattgttgt cattcacgac
      841 aatacgacta ctagaacaac gggagtagaa aaagtagtaa atgacactac ttacaaagat
      901 ttaaagaaat taagactact acatacaaag actgaaatac cattattaaa agatgtttta
      961 aagatggtta aaggtaaagt tcctgtttta gtggaagtta aagccgaaaa taaagtccca
     1021 cctgcacttg tcaccgaagt gttaaaagtt atcgaaaagt atccgtataa agataaacta
     1081 gcgatgcagt ctttcaaccc atatgttgcg cgtgacttaa aaaaggggag caccggtatt
     1141 cctgttgggc aactaatgag tgatgtactg tacggtcaat caaaattagt gcactttatg
     1201 tatcgttcac ttctagtcct taaaatatct aaaccagact tcttcaatta tgaagtaaaa
     1261 tatattcata agcgaaacat tcagagaaaa cgcaaacgct taccacttat aacatggaca
     1321 attgatgact acgataaaga aaaaacagct attgccctag ctgataacta tatttttgaa
     1381 catattgaaa taaaggagag ataatatgga actaaaactt aacatgaagc gcacattcct
     1441 cgtcggatta gcgtttatgt caattatgct gttctggacg atgtatgaca cactcgtggc
     1501 caaaatgctt gttgacaact ttgggctcaa tcaaaccttc tcagggttta ttatggcact
     1561 tgataacatc ttagcgttat tcttgctacc actatttggg cgaatcagcg acaaaacgcg
     1621 gaccaaatta ggcaaaagaa caccgtttat tattatcggt actttagccg cttctattct
     1681 ttttgttagc attgccttct ttgatttata tcaacaaatt gccgttacta aacacggttt
     1741 ctcattaatt gaatcaattg gcactgctga aaatccgtta taccaattca cacttggaac
     1801 tgaagtatat acaaacaatg tacgttctgc tcttgcaacc gagcgcgcac aattaatttt
     1861 ccaacaagta acaaaacaac aacctggttt acttattggc tttattgttg ttttattctt
     1921 tgtcttagtg gcaatgggaa tttaccgcag tcctgctgtt agcttaatgc cagacgttac
     1981 acctaaacca ctccgtagta aagctaatgc tgtcattaac tttatgggga caatgggtgg
     2041 tgcgtttgcc tatgtcctag tattcttact tgctagcgtt cctatctttg ataaaacggg
     2101 ctatgtcgct acttttgcaa caactagttt actaatgctt ttattaattg ctatctttgt
     2161 tgcgactgtt aaagaaaacg catggtataa agaaatggtc gaaatcactg aaaagtatca
     2221 ccttgattca ttagatattg atgacgaagg tttagatgta tcacaacata ttgatgacga
     2281 aaaaatgcca aaagatgtac gccgtagttt tattctcatc ctaagtgctg ttgtattatg
     2341 gtttatgggt tataacgctg cgactacaaa gttttctgtt tatgccttta accatttagg
     2401 acttgaacaa tttagtttac caccacttat tggcattatt accgctgcgt tagcgtttat
     2461 tccaattggt tacatttcac aaaaaattgg tcgtcgccgt atgattctct tcggtattgt
     2521 tgtgttaaca attgctttag gtagtgccgt ctttattact ggtgaaacta aagtattaat
     2581 gtacattatc atgggtcttg tcggcattag ttgggcttca attaatgtta atagttatcc
     2641 gatggttgtg gaaatgtcgc gtcacggtaa tatcggtgtt tacactggtt actactatag
     2701 cgctagtatg agtgcaatga ttattacacc gattctttct ggggcattaa tggatattac
     2761 cggttggtac ccagtactct ttatttatag tgcggtcttt atgggcctag cgttcattcc
     2821 aatgtacttt gttaagcacg gtgaacctgc gccagtagtg ttaaaagaaa aagcacaaaa
     2881 agacgaataa ttcgctaaat acactatcga aagatgaccc tagtggggtc atctttttta
     2941 ttattatcat tattactttt caattatttc ttactgaata attgattttt gcttatgtgt
     3001 tttgggatta attacttaaa atgctataat gatctttcaa gcgaggtttg atgttatgaa
     3061 aaagaagaaa acgatggtgc gcttgtgggg agatcacatt gtaatatcat cactgtttat
     3121 agggcttgct attagcctag ccttcgtggc attaacattt gttactattg aagtattagc
     3181 aaaacatgtt gcgatcattg ccgaaaaaaa gaaagatttt atttatgggt ttgggacatt
     3241 agcgattctt gttgcttttg tcattaataa tttttggatt aagccccaac gcaatattgt
     3301 taaatcaagt gatttaaaca agaaagaaag agagggctaa tttatggtta ttactttctc
     3361 tttaatttta caaatggtcg ggattgctct ccttatgacc gctttatatc tgattatcgg
     3421 tattatccct ggcactgatg aaacttcagt cttagtgcca atgacactta ttctagttac
     3481 tttaaaactt aacccacttg tgattatggc tggctttatt gctgcagttg ttactcttaa
     3541 tattgctgac tcgataccga cagcaataac gtcgatccca ggcggggtaa tgtcaacacc
     3601 gcttgttggt catagtcaaa cactaaagaa acacggactt acttcttata gtgtacagtg
     3661 gatgatgctt ggttctttaa tcggtgtctt agttagtttc cccattgccc tagcgttttg
     3721 gggtattttt gaatttattt ctgtacaaac tggattagaa ataagtaact ttattaagcg
     3781 ttacaatgaa tacatcttcc ttggtggcgc tattttgatg agtttaacaa gtaaggaaaa
     3841 agttgtttca cttttatcaa tcgttccgtt tggatttgcc actgctttct tacgtaatcc
     3901 aggtcttatt attaataata cgctcttaaa tacgcttatc ggtggccgga ctttatcaat
     3961 gacacctttc tttttaagta ttacgactgg tccacttgtg tttgcgttat ttgaactttt
     4021 cttccccagt gtatttaaaa accaaaaggt tgaaggaaag atgactgtta ttcttgatga
     4081 tgatgtaaag aaaacaaagc atggtcataa aaagatcatt gctaaaactg gaatcgctag
     4141 tgcgcttggt agtttgctct tcttcatgtc accggttggt gtcacaatga tgcttggtga
     4201 atttgcgacg aaagatgaaa aagacgaagt acaacaagct ttacaaggtg tttctgctgt
     4261 taacggttta tcaagcgcta cttatttagc aggaattatg atttcacttt tcgtcttcgc
     4321 tttccctatt tcaccagcgg cacttggtcc aggctccgtt atttttagtc ctggcggtcc
     4381 aattattctt gaaggtaatg ctgggttagc ggtaccaaaa ggttatttat taatcgccat
     4441 tgttattggt ttagtaattg gacttagctt aacagtgtgg cttggtatga aatattctca
     4501 taaaatgact gaacttgtat ttaagtatat ttcacaagaa acagtgctta tctttttgtt
     4561 ctcaattatc gcgcttcttt cttttgccga agctggttta tatggtttaa taattgtagt
     4621 tagtgttggc cttctaagtg gttggctaaa taaaaaagga gcgggttacg gtgtacagtt
     4681 tatggcactt tacgcaggtg ggttatttat tacaatcctc aaagcgctct tctaattagg
     4741 gactaattat ggatggatac ggtttagcaa aaattatatt aattggtgag cattccgtcg
     4801 tttacggtca tcccgctatt gcgattcctt tttatgcaat gcgaagcgaa gttgttttaa
     4861 ctccgcacga agagtttatt cttgatacga agtattacac tggcccactt aataatgccg
     4921 tagatgaatt acacggactt gttgctttaa taacacatat ggctgaaatt tataaaaaga
     4981 ttaagcctgt cattataagt gttcgttcaa gtttaccaga gaaaagcggc ttaggctcaa
     5041 gcgctagtgt tgctaaagcg gtaattgatg cttttaacaa tcattttcat cttgaattaa
     5101 cacatattga ttactttgaa ttattaaaaa tctctgaaaa tatatatcat cataacgcaa
     5161 gcggaattga cgcttctact gttatttatg aaaagccaat tatatatcaa agaccgcaca
     5221 taaagccact gaaatttaat attgatggct atttagcggt gttttattca aatacaaaca
     5281 gtgcgacaaa aagtgctgtc gcacatgtaa gcactcatcc tgaacgcgac attcatattg
     5341 atgctttagg ttcattaact gcaaacgcta aatgggcatt agaaaataat gacattacgc
     5401 ttcttgcaac gatgtttaac gctgctcaca cacatttaag agcgctaggt atttcaacgc
     5461 ctacacttga agaattacgc actaagctat tagacgcagg tgcgcttggt gttaaaatta
     5521 cgggtggggg aatgggcgga tgtttaattg ccctatttaa tgatgaagaa aaagcacttg
     5581 ccactaaaga aaaattaaag agctttccat catggatgat tgatttgagg aacatgtgat
     5641 gaaatataaa aaagcaaatg taaatatcgc tattcttaaa tattggggga aagttaatgc
     5701 tttaattaat attccttatc aaacaagtat ttccgttacc gcggataatt tttatactct
     5761 cactaatgtt gaattagatc caaaagccac taaagatatt gttattcttg atggtacagc
//
LOCUS       DAOPCD010000033         5643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_12371_length_5643_cov_3.500179, whole genome shotgun sequence.
ACCESSION   DAOPCD010000033 DAOPCD010000000
VERSION     DAOPCD010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12371_length_5643_cov_3.500179"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(611..901)
                     /locus_tag="PLQ55_01650"
     CDS             complement(611..901)
                     /locus_tag="PLQ55_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28673.1"
                     /translation="MHDRKTAFNFATWLFIYDFGKCDIRNPYPFLALLFKKMELDKDI
                     QDNLNNRFDTPYDRFLDLYVDVLISSKIINANEADYLIPDKDTMLLDELEKL"
     gene            complement(1202..1975)
                     /gene="trmD"
                     /locus_tag="PLQ55_01655"
     CDS             complement(1202..1975)
                     /gene="trmD"
                     /locus_tag="PLQ55_01655"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPK28674.1"
                     /translation="MKITILTLFPEMFTNFLETSIIKKARLQGHVTFDIVNIRDFADN
                     KWNRVDTPPVGGGAGMILQAEPIVKALESVDSGHKLLLSPRGNVLTNMKATALSQKEH
                     LIFICGHYEGIDERVNNFIDEEISIGDYILTGGELGAMVISDAAIRLLDEVISTESLD
                     IESFDYHLLEYPQYTEPYDFRGYTVPTILYSGNHAAIRKWRLKESLRITRERRPDLFK
                     KITKDNEIKKLLKEIDDDVIGEWEKDAIEKGSRFTKNKT"
     gene            complement(1972..2487)
                     /gene="rimM"
                     /locus_tag="PLQ55_01660"
     CDS             complement(1972..2487)
                     /gene="rimM"
                     /locus_tag="PLQ55_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPK28675.1"
                     /translation="MNKDYLKIGYIVETHALKGALKIKPTTSFAEERFEVGNKVLVKN
                     NTTSKTETLTIQSVREQKGLLIVTFAEIDNINIAENYLKGFLFIAKKDITLEEGYFFF
                     DDLLGMKVVLEDETLVGEVIEILEYAAYHTLRVKREDGSNILIPYIEEFIVSTDLASK
                     TIVFRPIEGML"
     gene            complement(2528..2782)
                     /locus_tag="PLQ55_01665"
     CDS             complement(2528..2782)
                     /locus_tag="PLQ55_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPK28676.1"
                     /translation="MRDYEALVRTIITPLVKNEQALLIRQTNEGHRIIINIFAESDDI
                     AVLIGRRGVVANALREVVSVAGKLDEEHVFLRFEALDENK"
     gene            complement(2785..3060)
                     /gene="rpsP"
                     /locus_tag="PLQ55_01670"
     CDS             complement(2785..3060)
                     /gene="rpsP"
                     /locus_tag="PLQ55_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012094939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPK28677.1"
                     /translation="MAVKIRLTRVGRTHDPHYRIVAADSRYARDGRIIEQIGHYDPKK
                     DLKEAVIDRELALKWLGNGAQPSATVKALFRAQGINEEFAKSKKSGK"
     gene            3214..4620
                     /locus_tag="PLQ55_01675"
     CDS             3214..4620
                     /locus_tag="PLQ55_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HPK28678.1"
                     /translation="MKNLLNKLFKFEERKANLTSEILGGLVTFLAMIYILPTNAGILS
                     DMGMDKSGVFVATAIVSAIVSLVMGLYGNFPIVLSAGMGVNAYLAYVVYGALGSWQAA
                     LLVMFFAGIIFAVISLSPLRKMMIDAIPNDIKYIISAGLGAFIAFVGFKSSGFIVDSP
                     ATLVSLGNLRDPMVLLSLFGVLLVVIFMFSSNKRLNQLAIPIAMIIVVVVSVAVWNIF
                     ALPEATDAGRPIAFHFTNIKDMFSNPTSWGGQGFEKVAFKIFDKETWGTALSNPSTYA
                     FIFSLVLVNLFDTTATILAVGRGAGIIDADGKMVGGHKVVAADSFGAVICAPLGTSTV
                     TSFSESGIAVEMGAKTGLAAVVAGFMFLLSAFIYPIFYIFTEPAVTSMALISVGGLIF
                     VGNLKDVNFDDRIIGFTAFFTIMLILLTYSISDGLGLGLIMYVIMMFVSKRGKEVKIP
                     MYVVAGVFVIYFILKALV"
     gene            4802..5149
                     /gene="rplS"
                     /locus_tag="PLQ55_01680"
     CDS             4802..5149
                     /gene="rplS"
                     /locus_tag="PLQ55_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPK28679.1"
                     /translation="MNKNLVNEINASQLRTDLPEFSSGDTVKVAVKIREGDKERIQFF
                     EGVVIAMQGAGVNHTFTVRKMSSGIGVERVFFTHSPVIDSVEVLRRGKVRRNKIYYIR
                     KLSGRKARIKEKK"
     gene            5272..>5643
                     /locus_tag="PLQ55_01685"
     CDS             5272..>5643
                     /locus_tag="PLQ55_01685"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HPK28680.1"
                     /translation="MVIEEVLINEEISEAEINRKNKPRLRKALSVTWTILLVTAIVVS
                     LFVTYVDLTYTPVYVDGPSMQPTLNDFHHRYYEFGLMDERPKARNNIKRGDIVVFRRN
                     GINIIKRVIALPLETIQIIDNP"
BASE COUNT         1688 a          959 c          999 g         1997 t
ORIGIN      
        1 gtttaaataa tagataagat ttaccacatc ttcgtaaacc agttatcact ttaacttttc
       61 cgttgccttt actatttatt aacttattaa tatagaaatc tctttttatc atattaccaa
      121 ctcctaaaat aaagtcataa tgttgactgt ttttcacgtg tattttaaca ttgacgtcaa
      181 aaatagtcaa caagttgatt gtttttaaca tcatttcgat acaagaaaag caagaatcct
      241 ttttagtaac ttcgccgcta attgctcaat taatttaatt ttatataata attacatagt
      301 gattccactt cgtttagcag ctttaaaaat ggtaatttta gttcaattat cgcgaaaaac
      361 catattttca gtcttttcat aattaaaaaa tgcattaata attgtatcaa aacagactaa
      421 gctagatggt atgagttgtc ctaattaata caatttcgag tgtaatcgtt caatcataca
      481 accccctaga ttattatcat tcagacagag gactttcgat gtaagaatag cggcgtgcaa
      541 agctataatt tttgttgcta ttttcttgct aatttagtat gcaatttata tagattatga
      601 aagtttatgt ttataatttt tcaagttcat ctaaaagcat agtgtctttg tcaggaatca
      661 aataatctgc ttcgtttgca ttaattattt tagaactaat aagaacatca acatataaat
      721 ctaaaaaccg gtcatatggt gtatcaaatc tattattgag attatcttga atgtctttat
      781 ctaattccat ttttttgaaa agaagtgcta aaaaaggata gggattacga atatcgcatt
      841 taccaaaatc ataaataaac aaccacgtag caaaattaaa tgctgttttt ctatcatgca
      901 cctcatgttt taaatacttc attcactccg ctactaaggt ttcccagcca aatgtagaaa
      961 ctagttggta aacagtattt ttaatttttt gtatatttgg tatttcttca ccatcaatac
     1021 aattttgtga aaataattct atgtttagaa tattttttac tatgctctta tcatttaact
     1081 tgatttatta atagttaaat ttccttaatc ctatttctca tcaaatttat tataggcatt
     1141 tattccgtgt ttttcagaag tattattgcc tattttgtaa atttataaat tatctttttt
     1201 tctaagtttt gttttttgtg aagcgtgacc ctttttcaat agcgtctttc tcccattcac
     1261 caataacgtc gtcatcaatt tctttaagca gttttttaat ttcgttatct tttgtaatct
     1321 ttttaaaaag atcaggccga cgttcgcgag taatacgtaa actctctttt agccgccact
     1381 tacgaatcgc tgcgtgatta ccagaataca aaatagtagg aacagtatat cctctaaagt
     1441 catatggctc cgtatattgc ggatattcaa gtaaatggta atcaaagctt tcaatatcta
     1501 aactttcggt gctaattact tcatcaagta aacgaatagc agcgtcacta atgaccatcg
     1561 cgccaagttc accgccagtt aagatgtagt cgccgattga tatttcttca tcgataaagt
     1621 tattcactct ttcgtcaatt ccttcataat ggccacagat aaagattaaa tgttcttttt
     1681 gacttagcgc tgttgctttc atattagtaa ggacattgcc gcgcggtgaa agtaacagct
     1741 tatgaccaga gtcaacactt tcaagtgctt taacaattgg ttcagcttga agaatcatcc
     1801 ctgctccacc accgacaggt ggagtatcaa ctctattcca tttgttatca gcgaagtcac
     1861 ggatattaac aatatcaaaa gttacatggc cttgaagtct cgctttttta ataattgatg
     1921 tttcaaggaa attggtaaac atttcgggaa ataatgttaa tattgtaatc ttcataacat
     1981 accctcaatt ggtcgaaata ctattgtttt gctcgctaaa tcagtggaaa cgatgaattc
     2041 ttcgatatat ggaattagaa tattactacc gtcttccctt tttactctta aagtgtggta
     2101 agcagcgtat tcaagtattt caataacttc acccacaagt gtttcatctt ctaaaacgac
     2161 tttcatccct aaaaggtcat caaagaaaaa gtaaccttct tcaagggtga tgtctttctt
     2221 agcgatgaaa agaaatcctt ttagataatt ctcggcaata ttgatgttat caatttcggc
     2281 aaaggtaact attaatagtc ctttttgttc acgaacactt tgaatggtta atgtttcagt
     2341 tttagaagta gtattatttt ttactaatac tttattacca acttcaaaac gttcctccgc
     2401 aaaagaagta gtcggtttaa ttttaagtgc accctttaag gcatgcgttt caacaatata
     2461 acctattttt aaataatctt tgttcataat tttattatat caatttaaaa aaactctcga
     2521 gtgagagtta tttattttcg tctagtgctt caaaacgtaa gaagacatgt tcttcgtcaa
     2581 gtttaccggc gacagacact acttcacgta atgcattagc gacaacaccg cgacgtccaa
     2641 ttaagacggc aatatcatca ctttcggcaa agatgttaat aataatacga tggccttcgt
     2701 tagtttgacg aataagtaaa gcttgttcgt ttttcactaa tggggtaata atcgtgcgca
     2761 ctaatgcttc gtagtcacgc ataattattt acctgacttt tttgattttg cgaattcttc
     2821 gttaatgcct tgggcgcgga ataaagcttt aacggttgca cttggttgtg ccccatttcc
     2881 taaccattta agcgctaatt cgcgatcgat aacggcttct ttaagatctt tctttggatc
     2941 atagtgtcca atttgttcga taatacggcc atcacgggca tagcgactat cagcggcaac
     3001 aatccgataa tgtggatcat gagtgcggcc aacacgggtt aatctaattt taactgccat
     3061 ataagtaatc ctccttttta taccgtgata agtttagcgt gactcgttta cttagtcaag
     3121 taattttact taactctagt taaattagta tgtatatctt ttatttttct taaaattgac
     3181 taaaataggt atattaaaaa caaggagcaa tttatgaaaa atctactaaa taagctgttc
     3241 aagtttgaag aaagaaaagc aaacttgaca agtgaaattt taggaggatt agtcaccttt
     3301 ttagcgatga tttatattct cccaacaaac gctggtattt taagtgatat gggcatggac
     3361 aaaagcggag tcttcgttgc tactgccatc gttagtgcta ttgttagttt agtgatggga
     3421 ctttacggta atttcccgat cgtcctaagt gcaggcatgg gggttaacgc ttacctagct
     3481 tacgtagttt atggagcgct aggttcatgg caagcagcct tgcttgtgat gttttttgcc
     3541 ggtattattt ttgcggtgat tagtctttca ccattacgga agatgatgat tgatgcgatt
     3601 cctaatgaca tcaaatatat tatcagcgca gggctaggtg ccttcattgc ttttgtcggc
     3661 tttaaaagca gtggttttat tgttgatagt cctgcgactt tagtgtcact tgggaactta
     3721 cgtgatccaa tggtcttact ttctttattc ggtgttttac ttgttgttat ctttatgttt
     3781 tccagcaata agcggttaaa tcaactcgca ataccaattg caatgatcat tgttgttgtt
     3841 gtttctgtcg cggtgtggaa tatctttgca ttaccagaag caactgacgc tgggcgtcca
     3901 attgctttcc attttactaa tattaaagat atgtttagta atccaacttc atggggtgga
     3961 caaggttttg aaaaagtcgc ttttaagatt ttcgataaag aaacatgggg cactgcttta
     4021 agcaatcctt caacatacgc ctttatcttc tcactagtac tcgttaatct ttttgatacg
     4081 acagcgacaa ttttagctgt gggacgtggc gctggtatta tcgacgctga tgggaagatg
     4141 gttggtggtc ataaagtagt ggccgctgat tcttttggtg ccgttatttg tgcaccgctt
     4201 ggtacaagta cagtaacttc attttccgaa agtgggattg ctgttgaaat gggtgctaag
     4261 actggtttag cggcggttgt agcgggattt atgttcttac ttagtgcttt tatttaccca
     4321 atcttttata tttttaccga accagcggtt acaagtatgg cacttatcag tgttggtggc
     4381 ttaatctttg ttggtaactt aaaagatgtt aactttgatg atcgcattat tggttttaca
     4441 gctttcttta caattatgct tattttactt acttactcga ttagtgatgg cttaggttta
     4501 ggacttataa tgtatgtcat tatgatgttt gttagtaagc gtggtaaaga agttaaaata
     4561 ccgatgtatg ttgtagcggg agtatttgtt atctacttta tccttaaagc tttagtttaa
     4621 acataagttt aataaacgct aatagcccac tttacgaaag agtgggcttt tttaaaattt
     4681 tagtaattac tctttacata agagtagtct tttggtatat taatggagcg caaagtcgct
     4741 gtggtccgct gccaaaaggt aagaacacga aaataatatt gcaggaagga gcgagttatt
     4801 catgaataaa aatttagtta atgaaattaa cgcttctcaa cttcgtaccg atcttcccga
     4861 attcagtagt ggtgatactg tcaaggtcgc tgttaaaatt cgtgaaggtg acaaagaacg
     4921 gattcaattt tttgaaggcg tggtcattgc tatgcaaggc gcaggcgtca accacacatt
     4981 cactgtccgc aagatgtcaa gtggtattgg tgttgagcgt gttttcttca ctcattcacc
     5041 agtcattgac agtgtggaag ttctccgtcg cgggaaagtt cgtcggaaca aaatttatta
     5101 cattcgtaaa ttatctggtc gtaaagcccg aattaaagag aagaaataag ttaaaatcat
     5161 ttatgagaga aagccagaaa actggctttt tttcttataa aacgagtatt tatttgtgat
     5221 aataagtagg aaaatgtaca tcaaatgtac atttcgttat ataattacta tatggtcata
     5281 gaagaagtac ttattaatga agaaataagt gaagcagaga taaatcggaa aaataaaccc
     5341 agactacgta aagcgcttag tgtgacgtgg actattttac tcgtgaccgc tattgttgtt
     5401 tcgctttttg tgacttatgt tgatttaact tacaccccag tttatgttga tggaccaagt
     5461 atgcaaccaa cactcaatga ctttcatcac cgctattatg aatttggctt aatggacgaa
     5521 agaccaaaag cccgcaacaa tattaagcgt ggtgatatcg ttgtatttcg gcgtaacggt
     5581 attaacatta ttaagcgtgt gattgctttg ccactagaaa ctattcaaat tattgataac
     5641 ccg
//
LOCUS       DAOPCD010000034         5385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_13007_length_5385_cov_3.444465, whole genome shotgun sequence.
ACCESSION   DAOPCD010000034 DAOPCD010000000
VERSION     DAOPCD010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13007_length_5385_cov_3.444465"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2134
                     /locus_tag="PLQ55_01690"
     CDS             <1..2134
                     /locus_tag="PLQ55_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPK28681.1"
                     /translation="VLNNDYEFKPTKQPASFPKTEKLDLIKSKMPFKSALKIGASALK
                     HKRFRLALSIILATSAFAMFGLTDAIANFKRTETTINTMLDLNIGYSSISKIERIKYS
                     DYYYSQQVGLDDDDLAELEKIDNRLDFFPVLNEALEYSSNFYSDSFDYYDPYRYGINT
                     IAHLKDADLTKANLSLLAGTLPTNKNEVAVPEYVFDSFKKYGYRSLTNSTKIAIDDPE
                     DLLNKTLNNKFVISGVYKTGLEVDRFIIENVDDDGSIYNTINTEVFRQYLLNGFHTTL
                     LVHEEYYNKLVEQNKVYSLPFNSNSYFQMGDVDRYQFTTRNFRRIESLKTNNDVILFN
                     GITHDNLGRDDIILSTSAAFFQGMEGGVHDSVQTALLVFVDNIKSTDENDLAIEQMLK
                     TNYQHYGFNSPLDVTAPSLWSEQEKTSFRNFYAQALLTFLYDENPFQPELIGREAIFA
                     VTSAQIKTDFAPFNIEYFLVAYESDGEKLKAAQDLRVVGFTFDYQYYIEAWESIIIYI
                     NEENAEVMNLTPEGAYFRAVTYHGNSRAIIQKLVKFNELGTKKDVGSFYRMNNEVSYL
                     VENVGDFLEIISRIFVVVGIIFAVFASLLFLNFITLSVAFKKQEIGILRAIGARGIDV
                     ATIFMNEAGIIALINLILSFMLAIIAGTTINNYIGKNINIYLSIVNVGLRQLLLLILI
                     AFGVAFISSFIPVFRLSRKKPIDAIRMR"
     gene            complement(2200..3237)
                     /locus_tag="PLQ55_01695"
     CDS             complement(2200..3237)
                     /locus_tag="PLQ55_01695"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPK28682.1"
                     /translation="MGIRFSVKSKIREWLSFRPRLKVATEWMYLLMLVLFSSFTYALA
                     FRLFISPSLETLHSVSGNQIVFVGGGVSGLSQNLVKLIHDIIGWQKVNPRVLQSILYF
                     TLNIPVFILGWLKIGRRFTIFSIINVLLASLFISIIPVSLEKAVIYDSQLTRTLYAGI
                     LAGFSAAVAFSGNVSSGGMDIIAYYFANKKSEGVGKYNVLFNVVVVGFYFVLNLIHPS
                     TNIDIANTQFTNTNVAITSFISSVTYLVMNSFVIDLINVRNKKSQVQIITNDEELAKI
                     LISNFPHGATTIKGIGVYSQSEKSVIYMTVSSNEVNDVVRLTQQVDPKAFINVYDLRQ
                     VFGKFYISPIK"
     gene            3455..>4125
                     /locus_tag="PLQ55_01700"
     CDS             3455..>4125
                     /locus_tag="PLQ55_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28683.1"
                     /translation="MKKILGAIGGFFVAIWRWIKETAWVQPLLIVGIIFGIIFAIPSV
                     VDGIRKIDERNNSAEKYYQQFQVSLAGAENSAADKLLDEIKQNSEGGSESLKGQKFFV
                     VFVQKDEACSACLDAREGFEYLADDGKALLDDGRKIELKTIFVDQELKRKDKEDWKKE
                     DSDPVDNYAETAFEAFLLRNAARFEEYAGDALNTHYYINDGITEAQIEDIESAFANNE
                     LDYGR"
     assembly_gap    4126..4135
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4136..4379
                     /locus_tag="PLQ55_01705"
     CDS             <4136..4379
                     /locus_tag="PLQ55_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28684.1"
                     /translation="NTHYYINGGITEQQVEDIESADVKRFQTPTILQIDFTDTAPQPG
                     VTNVFIGVQGAKKLDRAKYIADAWNYKGQFGPNYTV"
     gene            complement(4447..4872)
                     /locus_tag="PLQ55_01710"
     CDS             complement(4447..4872)
                     /locus_tag="PLQ55_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPK28685.1"
                     /translation="MGLVLFQPMILGMAGNIGTQSMGVTILSLHSGEIKIRKHFGREL
                     LIGLLNSFASGIVGFGIVFLFLTILGQDNALMYAAVVGLSLMCAMIISSLAGVLTPII
                     LLAVKLDEKAASGPLITTINDFFALQIYFLIATLLFTVL"
     gene            complement(4962..>5385)
                     /locus_tag="PLQ55_01715"
     CDS             complement(4962..>5385)
                     /locus_tag="PLQ55_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPK28686.1"
                     /translation="PHFISFPNTTTVKKATYNTIANAADNDEIDVIFFHDENNKYVGA
                     TTIKDLIAARSTTPISEVIDTNFPFVYEDEKITWALKMIRDYDILMLPVLTRDGEMIG
                     FVSETDALNLMAESHETMMRQLVKVAEDDDNLPPHKTP"
BASE COUNT         1809 a          946 c          925 g         1695 t
ORIGIN      
        1 ggtattaaat aatgattatg aatttaaacc gacaaaacaa ccagcatctt ttccaaaaac
       61 ggaaaaattg gatttgatta aatcaaagat gccctttaaa tcagcgctta aaattggcgc
      121 tagtgcactt aaacataaaa gatttagact cgcgcttagt attattttag ccactagtgc
      181 atttgcaatg tttgggttaa ctgacgccat tgctaatttt aaacgtacgg aaacaacgat
      241 taacacaatg ttagatttaa atattggata ctcctctatt tccaaaatag aacgcatcaa
      301 atatagcgat tattattatt cgcaacaagt agggttagac gacgatgatc tcgccgaatt
      361 agaaaaaatc gataatagac tagacttctt tcctgtactt aatgaagcac ttgaatactc
      421 tagcaatttt tatagcgaca gttttgatta ttatgaccca tatcgttatg gcatcaatac
      481 tattgcacac ctaaaagacg cagatttaac aaaagctaat ctcagcttat tagcgggcac
      541 attaccgacc aataaaaacg aagttgcggt tcccgaatat gtttttgatt cctttaaaaa
      601 gtatggatat cgctcactga ctaatagcac taaaattgca attgatgacc ccgaagattt
      661 gcttaataaa acattgaaca ataaatttgt aatttctggt gtatataaaa ctggtttaga
      721 agttgaccgt ttcataattg agaatgttga tgatgatggt agtatttata acacgattaa
      781 cactgaagtt tttagacaat atttattaaa tgggtttcat accacactac ttgtgcatga
      841 agagtactac aataaacttg ttgaacaaaa taaggtatat tctttgccct ttaactctaa
      901 ttcttatttc caaatgggag atgttgatcg ttatcaattt acaacccgta acttccgcag
      961 aattgaaagt ttaaaaacta acaacgatgt tattttattt aatggcataa cgcacgataa
     1021 tttaggtcgt gatgatatta tacttagcac ttctgccgct ttcttccaag gtatggaagg
     1081 cggtgtccat gattcagttc aaaccgcact tttagtattt gtcgataata taaagtcaac
     1141 agatgaaaac gaccttgcga ttgagcaaat gttaaaaact aattatcaac attatggttt
     1201 taatagcccc cttgatgtta ctgcaccatc tctctggagt gaacaagaaa aaacaagctt
     1261 ccggaatttt tatgcccaag cactactcac cttcctctat gatgaaaacc cgttccaacc
     1321 cgaactcatt ggtcgcgaag ccatctttgc tgtaactagc gcacaaatta aaactgactt
     1381 tgcgccattt aacattgaat atttccttgt tgcatatgaa agtgatggag aaaagctaaa
     1441 agcagcgcag gatttacgtg ttgttggttt tacttttgac tatcagtatt atattgaagc
     1501 gtgggagagt attattattt atattaatga agaaaatgct gaagttatga atttaacacc
     1561 tgaaggtgct tacttccgtg cagttactta tcacggcaat tcacgtgcta ttattcaaaa
     1621 acttgttaag tttaatgaac taggaacaaa aaaggatgtt ggttcttttt atcgcatgaa
     1681 taatgaggtt tcttatcttg ttgaaaatgt tggagacttc ttagaaatta tttctagaat
     1741 atttgtcgtt gttggcatta tcttcgcggt atttgcctca ctactcttcc ttaacttcat
     1801 tacactaagt gtggcattta agaagcaaga aattgggata ctacgtgcga ttggtgcacg
     1861 tgggattgat gttgctacta tctttatgaa cgaagcagga attattgctt taattaatct
     1921 catactctca tttatgcttg ccatcatcgc tggaacaaca attaataatt acattgggaa
     1981 aaatattaat atttatttat caattgtcaa tgttggccta aggcaattat tacttctaat
     2041 tctcattgcc tttggcgtag cgtttatttc tagctttatt cctgtcttcc gccttagtcg
     2101 caagaaacca atcgacgcaa ttagaatgcg ctagaagtat ttatgtgaca ttaaaaaatg
     2161 cgcttcttac aagcgcattt tctttaatgt tcacaatctt tatttaattg gtgatatgta
     2221 aaatttacca aaaacttgtc gcaagtcata gacattaata aaggctttag gatcgacttg
     2281 ttgtgttaag cgcactacgt catttacttc atttgatgaa actgtcatat aaataacaga
     2341 tttttcactt tgtgaataca caccaatacc tttaattgtt gtcgcaccat gcgggaagtt
     2401 ggaaataaga attttcgcga gttcttcatc atttgtaata atttgcactt gcgatttttt
     2461 attccgcaca ttaattaagt caattacaaa tgaattcatt acaagataag taacactaga
     2521 aataaaactt gtaatcgcta cattagtgtt cgtaaattgt gtattagcga tatcaatatt
     2581 agtgctcgga tgaattaagt tcaggacaaa ataaaaacca acgactacga cattaaagag
     2641 cacattatat ttaccaacac cttcgctttt cttattcgca aagtagtagg caataatatc
     2701 cattccgcca ctactaacat taccactaaa agcaaccgca gcgctaaaac cagcaaggat
     2761 gcccgcgtaa agtgtacgcg ttaattgcga gtcgtaaatt accgccttct ccaaactaac
     2821 tggaataata ctaataaata atgatgcaag taaaacattt ataatcgaaa aaatagtaaa
     2881 tctccgacca attttaagcc accctaaaat aaaaacggga atatttaacg taaagtacaa
     2941 aattgattgt aaaacgcgag gattaacttt ttgccaaccg ataatatcat gaattaattt
     3001 aactaaattt tggcttaaac cagaaacacc gccccccaca aacacaattt gattaccact
     3061 gacactgtgg agcgtttcaa gcgatggact aataaataaa cgaaatgcta aagcataagt
     3121 aaacgaacta aagagcacta acattaaaag atacatccac tcggttgcaa cctttaagcg
     3181 tggtcggaat gaaagccact ctctaatttt acttttgaca ctaaaacgaa tacccataat
     3241 tacctcacta tcttgaatta taggtttaat ttaataaact tgcaaaatat aactatttaa
     3301 aactattgtt ttaatgaaaa aacacattgt tttgctatta gatttcagaa actttagtaa
     3361 attgcttttt ttattattgc ttttgtttga aaatatatgt tgagagtgtt attattatac
     3421 gtgctctatt atttaataga aggaggaact tttcatgaaa aaaatcttag gtgctatcgg
     3481 tggtttcttt gtagctatct ggcgctggat taaagaaaca gcctgggtcc aaccactttt
     3541 aattgttgga attatctttg gaattatttt tgcaattcca agtgtcgtcg atggcattcg
     3601 taaaattgat gaacgtaaca atagtgcgga aaaatactat caacaattcc aagtctcact
     3661 tgctggtgcg gaaaatagtg ccgctgataa attacttgat gaaattaagc aaaattcaga
     3721 aggtggtagt gaaagcttaa aaggacaaaa attcttcgta gtttttgtgc aaaaagatga
     3781 agcatgcagt gcatgtcttg atgctcgtga aggctttgaa tatttagcgg atgatggtaa
     3841 agctttactt gatgatggcc ggaagattga acttaaaaca atctttgtgg accaagaatt
     3901 aaaacgtaaa gataaagaag attggaagaa agaagatagc gatccagttg ataattatgc
     3961 tgaaaccgct tttgaagctt tcttacttcg taacgctgct cgttttgaag agtatgctgg
     4021 ggatgcactt aatacccact actacattaa tgatggtatt actgaagcgc aaattgaaga
     4081 tatcgaatcg gcatttgcga acaatgaact ggattatggc cgcaannnnn nnnnntaata
     4141 cccactatta tattaatggc ggtattactg agcaacaagt tgaagatatt gaatcagcgg
     4201 atgtcaaacg tttccaaacc ccaaccatat tacaaattga ctttactgac acagcaccac
     4261 aacctggtgt tacaaatgtc tttatcggtg ttcaaggagc gaaaaaactt gatcgcgcta
     4321 aatacattgc tgatgcatgg aactataaag gtcaatttgg accaaattat actgtctaac
     4381 attattgaaa agttgaaaaa tgccggcgtt aagccggctt ttctttttac ttcttctact
     4441 aaattattaa agtactgtaa acaaaagtgt cgcgattaag aaataaattt gtaaggcaaa
     4501 gaagtcatta atcgttgtaa taagcggacc agacgctgct ttttcgtcta gcttaacagc
     4561 gagcaaaata attggcgtta acacacctgc taatgagcta ataatcattg cgcacataag
     4621 tgataagcct actactgcag catacatcaa agcattatct tgacctaaaa tagtaaggaa
     4681 taagaaaaca ataccaaacc cgacgatacc ggaagcaaaa ctatttaata atccaattaa
     4741 aagttcacgc ccaaaatgtt ttctaatttt aatttcacca ctatgtaagc ttaaaattgt
     4801 gacccccata ctttgtgtac caatattacc agccatccca agaatcattg gttggaataa
     4861 gactaagccc acaaaagtac ttaaaagtcc ttcgtatgat gtaagtgtgc cagcgattag
     4921 taagttaagt acgaccgcga cgagcggcca cggtaaccgt gttaaggcgt tttatgtggt
     4981 ggaagattat catcatcttc ggcaactttc actaattggc gcatcattgt ttcatgcgat
     5041 tccgccatta aatttaatgc gtcagtttca gaaacgaacc caatcatttc accatcacgc
     5101 gttaatacag gaagcattaa tatgtcataa tcacgaatca tttttaatgc ccaagtaatt
     5161 ttttcgtctt catacacgaa cgggaaatta gtgtcgataa cttcactaat tggagttgtt
     5221 gaacgagcgg caattaagtc tttaatggta gtagccccta catatttatt attttcatcg
     5281 tggaagaaaa taacatcaat ttcatcatta tcagcggcat tagcaatcgt attatacgtt
     5341 gcttttttaa ctgttgtcgt atttgggaaa gaaataaagt gtggc
//
LOCUS       DAOPCD010000035         5310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_13222_length_5310_cov_2.962702, whole genome shotgun sequence.
ACCESSION   DAOPCD010000035 DAOPCD010000000
VERSION     DAOPCD010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13222_length_5310_cov_2.962702"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1981)
                     /locus_tag="PLQ55_01720"
     CDS             complement(<1..1981)
                     /locus_tag="PLQ55_01720"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPK28687.1"
                     /translation="MKKRNIFTKFSLTAILLSALFAFSSVRASSNIVRTEAWSGTQTS
                     TEGTYYSTVGSETGTALLNKLNTITGNPTPQTSYDWYRYEAADEAENESNKVLLIYSR
                     QKVLKTAHVSGSTGWNREHTYPDSKISGAAKSDNHHIFADDNRTNSTRGNMPFGVVAQ
                     TSTNRVKDGYGNLTDCYKDGTYFYPNEPARGEVARATMYVNMRYGYSVTGNFQSIALM
                     LEWHLQNPVSNREIYRNNTVHTMQKNRNPFIDHPEYACRIWGNTNSETQAICGAAQQV
                     DVTSVSVSPSSGSINLLDVNKTLQLSANVLPANATNKSVNWSTSDSSIATVDANGLVT
                     GKAVGNVTITATSVSNPSVKGTASIAVSNTSIPVTGVTISDKSFTLVKDATKTLTATV
                     SPANASNKNVTWSSSNTSVATVSSSGVVTGVSAGNATITVTTVDGGKTDTANVTVANA
                     PPTQSETTTYTFSTKSWTATPANWQSMYPADGFETDQNYLRGMQVLGNNTAYGNSPYF
                     DNVKKVIFGTAASSSGAGTYSAYLVTSSTSKLKDGTQIGTDVTVSKPGATIIERTFEL
                     TTPASGHVQAIIDATSNSLYLKYVTIEVATPINYQGEADLWANGFLESTSAGCSSQSQ
                     STLSSIWPNLSNEYNALAAEVKAIIEGSTPNSAGSE"
     gene            complement(2043..4625)
                     /locus_tag="PLQ55_01725"
     CDS             complement(2043..4625)
                     /locus_tag="PLQ55_01725"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPK28688.1"
                     /translation="MAITLAKKKTIVGVFFSQMNNAMIYILLVAAVISFVMALIEGDG
                     SFFEPILILAIVLLNAVIGAIQELKADKALEALEKLSAPTTVVRRDGELKEIKASELV
                     PGDIVIIEDGRTIPADLRLISSANLKVSEASLTGESVPVEKDANIVYDRPMPIGDRKN
                     VVYMSTPVVYGRGEGVVIGTGLQTEIGQIAKMLQTTKQEETPLQKRLADLSKLLGIIT
                     FVLVALLFIIALIKDPTWDNIKNMFLLSISLAVAAVPEGLPAVVTITLALGVQRMVKV
                     HTITRRLPSVETLGAVSVICSDKTGTLTQNVMTVVSAYTNEKQYEVEDFSADTVGELA
                     DGLSLCSDASVDHGVYGDPTEIALVNFANLIDHHKDDLEAATPRVEELPFDSVRKMMS
                     TLHKTGKTYTQYTKGAMDNVLELTTHISVNGKVREIKKSDITAINDVASKMAKRALRV
                     LALAKKEVPFAGISEENLVFVGLVGMVDPPRPEAIPAVENLKGAGITTIMITGDHADT
                     AYAIAHELGITDSPSNVLTGAELDALNEDALKDALLTKRVFARVSPENKVQIVNALQA
                     NGEIVAMTGDGVNDAPSLKTADIGIAMGITGTDVAKGAADMVLTDDNFASIEKAVQEG
                     RGIYANIRKTVWFLLSSNFGEVITMLVAVLVGYPFPLSALHILWVNLITDGVPALALG
                     ADSKDPDIMKEKPRDKKESLFANGGYFITIFFGSVIALITTLAFLFIPVVQHGMRSVQ
                     DINTFFAENPELKVRAMTYAFTALGISQLFHMFGMSNLNKTFFRTFTKKKWFLWVAFF
                     IGFLLQIAVTEIHALTVAFGTVELTFIEWVFLALLSTTPLVIHELRVFFRFIREKIKN
                     RKSH"
     gene            complement(4633..5310)
                     /gene="rplA"
                     /locus_tag="PLQ55_01730"
                     /pseudo
     CDS             complement(4633..5310)
                     /gene="rplA"
                     /locus_tag="PLQ55_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545989.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
BASE COUNT         1569 a         1183 c          944 g         1614 t
ORIGIN      
        1 cttcactacc agcagagttt ggtgttgagc cttcaataat tgctttaact tctgctgcta
       61 atgcgttata ctcattactt aaatttggcc aaatactact taatgtactt tgtgattggc
      121 tactacaacc agcacttgta ctttctaaga agccatttgc ccataaatca gcctcccctt
      181 gataattaat gggtgtcgct acttcaatcg ttacatattt taagtacaaa gaattagaag
      241 tcgcgtcaat aatagcttga acgtgacccg acgctggtgt tgttaattca aaagtacgct
      301 caataatagt cgcacctggt ttactaacgg ttacatcggt accaatctgt gtcccatctt
      361 ttaattttga ggtactagaa gttactagat aagcactata agttcctgcc ccactacttg
      421 aagctgctgt tccaaaaata acctttttca cattatcgaa atacggacta ttaccatagg
      481 ctgtattatt acctaaaacc tgcatacctc ttaaatagtt ttgatcagtt tcaaaaccat
      541 cagcaggata catactctgc caattagcag gtgtagctgt ccaacttttg gttgaaaatg
      601 tataagttgt tgtttcactt tgggttggcg gtgcattggc gactgttacg tttgcggtat
      661 cagttttacc accatcaact gttgtgacag ttattgtggc gtttcctgcg cttactccag
      721 taacgacccc gctactagaa acagtcgcaa cacttgtatt tgatgatgac catgttacat
      781 tcttatttga tgcattcgct ggacttactg tcgcagttag tgttttagta gcgtctttaa
      841 ctaatgtgaa cgatttatca ctaatagtga caccagtaac aggaattgat gtattactaa
      901 cagcaataga tgctgtacct ttaactgatg gatttgatac cgaagtagca gtaattgtga
      961 cgtttcccac tgctttaccc gtgactaaac cgttagcgtc aacagtcgca atacttgagt
     1021 ctgaagtgct ccagttaaca cttttattag tagcgttagc gggtaaaaca ttcgcactta
     1081 attgtaatgt cttattaaca tctaataagt taatgctacc acttgacggt gacacactta
     1141 cactagtaac atcgacttgt tgtgcagccc cgcaaatagc ttgtgtttca ctattagtgt
     1201 taccccaaat ccgacatgca tattctggat gatcaataaa aggattacga tttttttgca
     1261 ttgtatggac ggtattatta cgatatattt cgcgatttga tacaggattt tgtaaatgcc
     1321 actctagcat aagtgcaata ctttggaagt taccagtcac actataacca taacgcatat
     1381 taacatacat cgtagcgcgc gctacttcac cacgagcggg ttcgtttgga taaaaataag
     1441 taccatcttt atagcaatct gtcaaatttc cataaccgtc tttaacccga tttgtgctag
     1501 tttgagcaac aacaccaaat ggcatgttgc cacgagtact atttgtgcga ttgtcatcag
     1561 cgaaaatatg atggttgtcc gatttggcag cacccgatat tttggagtca ggataagtat
     1621 gttctctatt ccaacctgta cttcctgaaa catgggccgt tttaagtact ttttgtcgcg
     1681 aataaattaa aagcacttta ttgctctcat tttccgcctc atccgctgct tcgtagcgat
     1741 accaatcata actagtttgc ggagtcggat tacccgtaat tgtatttaat ttattaagta
     1801 aagctgtgcc cgtttcacta ccaacggtgg agtaatatgt accttcagta ctagtttgtg
     1861 tcccgctcca cgcttctgtt ctaactatat tactactagc gcgtacacta ctaaaagcaa
     1921 agagcgcaga tagtaaaatt gcagttaaag aaaatttagt aaaaatattt cgttttttca
     1981 tatacttatc ctcacaagga aatttattat aactttacta atgtcataaa gcaatagtgt
     2041 tattaatgac tttttcgatt tttgattttc tcacggataa agcggaagaa aactcttaat
     2101 tcatgaatta caagtggtgt tgtactaagt aatgctaaaa atacccactc aataaatgtt
     2161 aattcaactg taccaaacgc cacagttaaa gcatgaattt cagttaccgc aatttgtaaa
     2221 aggaagccaa taaagaaagc aacccataag aaccactttt tcttcgtaaa tgttctaaag
     2281 aatgttttat ttaaatttga catgccaaac atatggaaga gttggctaat acctaatgca
     2341 gtaaaagcat aagtcattgc tctaacttta agttcaggat tttccgcaaa gaaagtatta
     2401 atatcttgaa cactacgcat cccgtgttgc actaccggga taaataagaa cgccaaagta
     2461 gtaattaaag caataacact tccgaagaat atcgtaataa agtaaccacc attagcgaat
     2521 aatgattctt tcttatcacg cggtttttct ttcataatgt ctgggtcttt cgagtcagcg
     2581 ccaagtgcaa gcgccggtac accatcagta attaaattaa cccataaaat atgtaatgcc
     2641 gaaagcggga aaggataacc tactaagacg gcaacaagca tcgtaataac ttcaccaaag
     2701 ttacttgata ataaaaacca cactgtttta cgaatattag cgtatatacc gcgtccttct
     2761 tgcaccgctt tttcaatact cgcaaagtta tcatcagtta ataccatatc agcggcacct
     2821 ttagccacat cagttccggt aatacccatc gcaatcccga tatcagcagt ttttaaacta
     2881 ggagcgtcat taacaccgtc ccccgtcatc gctacaattt caccattagc ctgtaaggca
     2941 ttaacaattt gtactttgtt ttcgggagat acacgggcaa agacacgctt agtaagcaaa
     3001 gcatctttta aggcatcttc atttaaagcg tcaagctccg cgccagttaa aacattactt
     3061 gggctatcag taataccaag ttcatgggca atcgcgtaag ctgtatcagc gtgatcgcca
     3121 gtaatcataa ttgtcgtaat acctgcaccc tttaaatttt ccacagcagg aattgcttct
     3181 ggacgcggtg gatcaaccat tcctaccaag cctacaaaaa caaggttttc ttcacttata
     3241 ccggcaaaag gcacttcttt cttcgctaaa gctaacacac gaagtgcacg tttagccatt
     3301 ttactagcga catcattaat tgccgtaata tcactttttt taatttcacg aactttacca
     3361 ttaacactaa tatgggtcgt aagttctaaa acattatcca ttgccccttt tgtgtattgg
     3421 gtatatgttt taccagtttt atgtaatgtt gacatcatct ttcttacact atcaaatggt
     3481 aattcttcaa cacgcggtgt tgccgcttct aaatcatctt tatgatggtc aatcagatta
     3541 gcaaaattga caagcgcaat ttccgttggg tcgccataaa ctccgtgatc aacacttgcg
     3601 tcactacaaa gactaagacc atcggctagt tcaccgacag tgtcagcact aaagtcttcc
     3661 acttcatact gtttctcatt agtgtatgca ctaacaacag tcatcacatt ttgtgttaaa
     3721 gttccagttt tatcactaca aataacactg acggcaccaa gcgtttcaac gctcggaagt
     3781 cttctggtaa ttgtatgaac tttaaccatc cgttgcacac ctaacgctag tgtaattgtc
     3841 acaaccgctg gtaagccttc tgggaccgcc gctactgcta aagaaataga aagcaggaac
     3901 atatttttaa tattgtccca agttggatct ttaataaggg caataataaa taaaagcgcg
     3961 actaatacaa aagttataat ccctaataat tttgataagt cagcaagcct tttttgtaat
     4021 ggtgtttctt cttgcttcgt tgtttggagc atcttcgcaa tttgaccgat ttcagtttgt
     4081 aaaccagtac caattacaac cccttcaccg cgaccataga caacaggagt cgacatataa
     4141 acaacatttt tacgatcacc aattggcatt ggacggtcat aaacaatgtt tgcgtctttt
     4201 tccacaggaa cactttcacc tgtaagacta gcttcgctaa ccttaaggtt tgcgctacta
     4261 attaagcgta agtccgcggg tatcgtacga ccatcttcaa taataacgat gtcacctgga
     4321 acaagttcac tagccttaat ttctttaagt tcaccatccc gtcgtacaac tgttgtaggg
     4381 gcactaagtt tttctagtgc ttcaagtgct ttatccgctt ttaactcttg aatagctcca
     4441 attactgcat taagtaatac aatcgctaaa attaaaatag gttcaaaaaa ggaaccatcc
     4501 ccttcaatta gcgccatcac aaaagaaata acagcagcga caagtaatat ataaatcatc
     4561 gcattattca tctgcgagaa aaagacacca acaattgttt tctttttcgc tagagtaatc
     4621 gccatttggt gcctacctag tcagagacat gtcgagggga atgcctggcg ccattgtggc
     4681 tgaaatcacc aggctcttga tataaacgcc tttggctgtt gcaggctttg cacgacgcac
     4741 tgagtcgata aatgccatca cattgtctgc aagctttgcg ctgtcaaagg agagtttgcc
     4801 tacatgggcg tgaacattag cacctttgac tatccgatag ctaatcttac ccgcctttaa
     4861 ctccttaatc actgcagcag gcgtggcagt caccgtgcca tttttagggc ttggcatcaa
     4921 acctcgaggc cccaaaacac gagcaagacg accgacaagt ggcatcatat cgggtgttgc
     4981 gacaatagcg tcaaaatcga gccagccacc ttgaattttt tcaccctact cctctgcgcc
     5041 aacataatcg gccccagcct ctttggcttg cgcctccaat tctcccttgg caaagacaag
     5101 cacgcgaaca tcgcgacccg tcccgtgggg aagaactact gtaccgcgca ccgcttggtc
     5161 agagtacttg ggatcgacgc ccagattcat gtgaacttca acagtttgat cgaattttcg
     5221 gtcgcatttt ttcagcaaag aaacagcatc ttcgatactg tattggcgac caggttcaat
     5281 caatgcagaa acgctgcgat atttttttcc
//
LOCUS       DAOPCD010000036         5309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_13226_length_5309_cov_4.159498, whole genome shotgun sequence.
ACCESSION   DAOPCD010000036 DAOPCD010000000
VERSION     DAOPCD010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13226_length_5309_cov_4.159498"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLQ55_01735"
     CDS             complement(<1..212)
                     /locus_tag="PLQ55_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK28689.1"
                     /translation="MKLLKTVKIINWHYFWNQTLNFEQIVFLTGVNASGKSTLIDAMQ
                     VVLLGDTSGRHFNKAAAEKSNRTLRG"
     gene            complement(209..847)
                     /locus_tag="PLQ55_01740"
     CDS             complement(209..847)
                     /locus_tag="PLQ55_01740"
                     /inference="COORDINATES: protein motif:HMM:NF025205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HPK28690.1"
                     /translation="MFEEAFLQLTRADQDAFAKAINELLLNSFIVRDEFDRREKTIKI
                     NPTYRFIERYYDLINAYLSFAGWRVDKDLILGVFSLVNEYNHNRLRFDREVSLVLFVL
                     RLIYEEERKEGGHVGDSVYLTTPLVMKVMHDRGIGFPNKRLSGRLIGRCLKEIAAHNI
                     IARVSGSYDEGNVTFYLLPSITFAVDNEKIVAISEALEKLALRDAGTEGGNV"
     gene            complement(849..2264)
                     /locus_tag="PLQ55_01745"
     CDS             complement(849..2264)
                     /locus_tag="PLQ55_01745"
                     /inference="COORDINATES: protein motif:HMM:NF039513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HPK28691.1"
                     /translation="MSLFTIIPSNFFSILASKNKEIYAEALLLLHHSIQDNELLIKKR
                     DFTRNLRDRLNELALSVDFDAEEEGDEDEIVNHSDKAAFIVRRLEECGWIDVEVNPDN
                     FEEYIALPSYSINVISLLNDLVKESTAEYVSLVHATYSELKLEDDMRDEFMYATLVRA
                     FENTKRLNVELITLAHSIRIFQNRLGKVFSTNNILSDYFDLYKTRVSDRYYHPLKTFD
                     SVAKFKRPILQILQKWLYDDDIRQQLVTQSLMWSRNKDAKAAEADIINKINYIADMYE
                     RVNETIDQIDQKHYEYTKASASKILYLNAADKSIKGHLDYIFKTYAETLSKGESVAPI
                     LNGINQSITFQQHGYITPDSLTLPIVRQLRAESIPMSLTDFDDVRDMLMQGFLDETSS
                     QFSNDAIFEFMDIAFDEESEVHISDIALPSFDAFLLLILATIKTSDEQCFYIIETEEG
                     SVSSYGYRLPNLTFVRKEITL"
     gene            2391..3458
                     /locus_tag="PLQ55_01750"
     CDS             2391..3458
                     /locus_tag="PLQ55_01750"
                     /inference="COORDINATES: protein motif:HMM:NF039499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HPK28692.1"
                     /translation="MANYELSRNTVSDLLSDNIQISPNTNEKLDYFRKAIKNTYPDYQ
                     KKFRHRARSFAVFAEIIIKRHNHTIKNNSIEHQKTYFKNDAYIYHIIEDFILAEEAKQ
                     NPEHTFTRDEYVDPTILQFENLIDHRYQNLLRYDFQKIKDPKLTLYNLTSRFFQELVS
                     GIMLLEREFYNDSFIIWRSLLETTTTLLILYENDNLVGKFNERRNIALMRVKVVDASR
                     QTLKSKAKETKQQLGFKGVPDYVAERYGWAGDLFKSRDYSLRTLLERINMVDLYSHYA
                     FASLFVHEYLISPEDLRLEIDFEKYLLSLYFKLYEAVRIKINDFTNDLDEVKKLEQGV
                     RKEVNNFKAQFNDFSTRIQTT"
     gene            3455..3904
                     /locus_tag="PLQ55_01755"
     CDS             3455..3904
                     /locus_tag="PLQ55_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28693.1"
                     /translation="MKHKRIYTSFRGLLLIYLPIYLLMSIIFYLIVGIKFPPTLNHYL
                     TVGGWTIITALYLIFGYKNSYYELTKHEIVHHKGSNILYYKFDDIIYIDKVYSGKKLS
                     IRFFTRIGDERYLAHDPKKKVYNTMLERCTNLIDKEELLRRFPKIKY"
     gene            complement(3894..4589)
                     /locus_tag="PLQ55_01760"
     CDS             complement(3894..4589)
                     /locus_tag="PLQ55_01760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPK28694.1"
                     /translation="MKTLKANSVDLIFADPPYFLSNDGLSIRSGKVVSVNKGEWDKRD
                     NYDSTYMFTYSWLKECHRILKPTSSIWVSGTHHNIFEIKKVMDDLGFRIINLVIWQKT
                     DPPPLIYKNKLKFSHEILIWAKKGRSHFFNYDLAYSLRNSELGDVWKFSAVQNHEKKF
                     GYHPTQKPERLLERIIRLTTNKNDVVLDPFMGSGTTCFVAKKLQRQYIGIELEEEYFS
                     IAKRRIDSIKANT"
     gene            complement(4657..>5309)
                     /locus_tag="PLQ55_01765"
     CDS             complement(4657..>5309)
                     /locus_tag="PLQ55_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009007620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HPK28695.1"
                     /translation="LLLAKRENTIKINTSETMYDFDFKNTNYSIEEYANFMDKTGLFN
                     LLKNRILRSLLDYVTGIEVGMDTNARKNRMGKAMEDLVEEHLKKKGFVRDVTYFKEMS
                     RSEIQSKFGLDLSNITNEGKAEKRFDFVIKTKDHVYAIETNFYSSSGSKLNETARSYE
                     LLARESATVQGFTFIWITDGLGWRSARNNLRQTFEVTRHIYNIADLENDVLAVVLK"
BASE COUNT         1758 a          963 c          822 g         1766 t
ORIGIN      
        1 tatccgcgta atgtccggtt tgatttttcc gcggcggctt tattaaagtg gcggccggaa
       61 gtatcaccaa ggagcacaac ttgcattgcg tcaataagtg ttgacttccc gctagcgttc
      121 accccagtca ggaagacgat ttgttcaaaa tttaaggttt gattccaaaa atagtgccaa
      181 ttaataattt tgactgtttt taataatttc atacattacc tccctcggtg cctgcatcgc
      241 gtaaggctaa tttttcgaga gcttcgctaa ttgcgacaat tttttcatta tcaacggcaa
      301 aagtaatcga tggtaataag tagaaagtaa cattaccctc atcataactt cccgagactc
      361 tagcaataat attatgtgcc gcgatttctt ttaaacaacg accaattaag cgcccactta
      421 ggcgtttatt tggaaaacca atgccacgat catgcattac tttcatcaca agtggtgttg
      481 ttaagtacac agaatcgcca acatgtcccc cttctttccg ttcttcttca taaattaggc
      541 gcaagacaaa taacactaag cttacttcac gatcaaagcg taagcggttg tgattatatt
      601 cattaacgag gctaaataca cctaatatta aatctttatc aacacgccag cctgcaaaac
      661 ttaaataggc attaattaag tcgtaatagc gttcaataaa ccgataagtc ggattaattt
      721 taattgtttt ttcgcgacga tcaaattcat cacgcacgat aaaactatta agtaataatt
      781 cattaattgc tttagcaaag gcgtcttgat ctgctcttgt taattgtaaa aaggcttctt
      841 caaacatatt ataaggtaat ctcctttcgt acgaatgtta agttcggtaa tcggtaacca
      901 tagctactaa cactaccttc ctcggtttcg attatgtaaa aacattgttc atcactcgtt
      961 ttaattgttg ctaaaattaa aagtaagaat gcgtcaaatg acggtaaagc aatatcacta
     1021 atatgtactt cactttcttc atcaaacgcg atatccataa attcaaagat tgcatcatta
     1081 gaaaattggc tacttgtctc atcaaggaac ccttgcatta acatatcacg cacatcatca
     1141 aagtcagtaa gtgacatcgg aatactttca gcgcgtaatt gacgaacaat tggtaaggtt
     1201 aatgaatcag gggtaatgta accatgttgt tggaatgtaa ttgattgatt aatcccgtta
     1261 agaatcggcg cgacactttc acctttactt aatgtttcag cataagtctt aaaaatataa
     1321 tcaaggtgac ctttaatcga tttatccgca gcatttaaat ataaaatctt tgaagcacta
     1381 gctttcgtgt attcatagtg tttttgatca atttggtcaa ttgtctcatt aactctttca
     1441 tacatatcag caatgtagtt aattttatta ataatatcgg cttcggccgc ttttgcgtct
     1501 ttgttgcggc tccacattaa tgactgtgtc actagttgtt gacggatatc atcgtcatac
     1561 aaccactttt gtaaaatttg taaaattggt cgcttaaatt tagcgacact atcaaatgtt
     1621 ttaagcgggt gataataacg gtcactcacg cgcgttttat ataagtcaaa ataatcactt
     1681 aaaatgttat tagtgctaaa tacttttcct aagcgatttt ggaaaatgcg gatgctgtgc
     1741 gctaaggtga taagttcgac attaaggcgt tttgtatttt caaatgcccg cactaatgtc
     1801 gcatacataa attcatcgcg catatcatct tctaacttaa gttcgctata agtcgcgtgc
     1861 acaagcgaaa cgtattcagc agtgctttct ttcactaaat cgtttaataa ggaaataaca
     1921 ttaatactat aacttggaag cgcaatgtat tcttcaaaat tatcaggatt aacttcaaca
     1981 tcaatccagc cgcattcttc taaacgacga acgataaatg ccgctttatc gctatgatta
     2041 acgatttcgt cttcatcgcc ttcttcttcc gcgtcaaaat caacactaag cgctagttca
     2101 tttaaacggt cacgcaaatt acgagtaaaa tcgcgcttct ttattaataa ttcattgtct
     2161 tgaattgaat ggtgtagtaa aagaagtgct tcagcatata tctctttatt cttactcgca
     2221 agaatcgaga agaagttact aggtatgatt gtaaatagac tcaaaaaaaa taatctcctt
     2281 cggttatatg ttttatgtta acatataaaa aaatgaattg gtattaaact tgtataataa
     2341 aagagaaata tttaatttcg tttaaaataa taatagaaag gcggtaacat atggcaaatt
     2401 atgaacttag tcgcaatact gttagtgacc ttttaagtga taatattcaa atttcaccaa
     2461 acacgaatga gaaacttgat tacttccgta aagcaattaa aaatacttat cctgattacc
     2521 aaaaaaagtt tagacatcgt gctcgttcat ttgcagtatt tgcggaaatc attatcaaaa
     2581 gacataatca tacgattaaa aataacagca ttgaacatca aaaaacatat tttaaaaatg
     2641 atgcttatat ttaccatatc attgaagatt ttatcctagc ggaggaggct aaacaaaatc
     2701 ctgaacacac ttttacacgt gatgaatatg ttgacccgac aatcttacaa tttgaaaact
     2761 taattgatca tcgttatcaa aatttattaa gatatgattt tcaaaaaata aaagatccaa
     2821 aactaactct ttacaactta acaagccggt tctttcaaga attagtgtca ggaataatgc
     2881 tcttagaacg cgaattttat aatgactcct tcatcatttg gcgcagtctc ttagaaacaa
     2941 caacgacctt gttaatactt tacgaaaacg ataatctagt tggtaaattt aatgagagac
     3001 gcaacattgc gctgatgcgt gttaaggtcg tagatgcgag tcggcaaaca ctaaaaagca
     3061 aagcaaaaga aactaaacaa caactaggct ttaaaggtgt gccagattat gttgcagaac
     3121 gttacggttg ggcgggtgat ttatttaaat cgcgcgacta ttccttacgg acactacttg
     3181 aaagaattaa catggttgac ctttattcgc attatgcctt tgcctcgtta ttcgtccacg
     3241 aatatttaat aagtccagaa gatttacggc tcgaaataga ttttgaaaaa tatttattat
     3301 cactttactt taaactttat gaagccgtgc ggattaaaat aaatgacttc acaaatgacc
     3361 ttgatgaagt taaaaaatta gaacaaggag tgcggaaaga agttaataat tttaaagcac
     3421 aatttaatga ctttagtacg cgaattcaaa caacatgaaa cacaaacgaa tttacacaag
     3481 ttttagagga ttacttttaa tttatttacc aatatattta ttaatgagta tcatcttcta
     3541 tctcatagtt ggtattaagt ttccaccaac cttaaatcac tatctaacag tgggtggctg
     3601 gacaattatt actgcgttat atttaatatt tggttataaa aatagttatt atgaactaac
     3661 aaaacatgaa atagtgcacc ataaaggtag caatatcctt tattataaat ttgatgacat
     3721 catttatatt gataaagtgt attccgggaa gaaacttagt atccgcttct ttacgcgaat
     3781 tggcgatgag cgctatttag cgcatgatcc aaagaaaaaa gtgtataaca cgatgcttga
     3841 acgctgcact aatcttatcg ataaagagga attattacgc agattcccaa aaattaagta
     3901 ttagctttta tactatcaat ccttcttttt gcaattgaaa aatattcttc ttctaattca
     3961 ataccaatat attgacgctg aagtttttta gcgacaaaac aagtagtgcc actacccata
     4021 aatggatcta gtaccacatc atttttgttt gtggttaatc gaatgattct ttctaataat
     4081 ctttcaggct tttgtgtggg atgataacca aattttttct catgattttg tactgccgaa
     4141 aatttccaaa catcgccaag ttcactatta cgcaaactat aggctaggtc ataattaaaa
     4201 aagtgtgacc ttcctttttt agcccaaatt aatatttcat gcgaaaactt taatttgttt
     4261 ttataaatta atggtggtgg gtctgttttt tgccagatta ctaaattaat tatccgaaaa
     4321 ccaagatcat ccatcacctt tttaatttca aaaatattat gatgagtgcc acttacccag
     4381 attgaacttg ttggttttaa aatcctgtga cattctttta accaactata agtgaacatg
     4441 tatgtactat cgtaattatc tcttttgtcc cattcacctt tatttactga cacaacctta
     4501 ccacttctaa tacttaaacc gtcgttggaa agaaagtagg gaggatcagc aaaaattaaa
     4561 tctacactat ttgcttttag tgttttcatt actttcgtgc aatcacctaa atacaacata
     4621 ctaataatta caaatgtaat tactaatatt tctcatttat tttaggacaa ctgctaaaac
     4681 atcattttct aaatcggcaa tattataaat atgacgagta acttcaaatg tttgacggag
     4741 attatttcta gccgaacgcc accctaagcc gtctgttatc caaataaagg taaaaccttg
     4801 aactgttgca ctttctcttg ctaaaagttc ataacttctg gcggtttcat taagttttga
     4861 gcctgaagaa gaataaaaat tcgtttcaat cgcatagaca tggtcttttg tttttattac
     4921 aaaatcaaaa cgcttttcag cttttccctc gttagtaata ttagataaat ctaatccaaa
     4981 tttactttgg atttcacttc gactcatttc tttaaaataa gtaacatcgc gcacaaagcc
     5041 cttcttttta agatgttctt ccactaagtc ttccattgct ttgcccatac ggttcttacg
     5101 ggcgttcgta tccatgccga cttctattcc agtaacataa tcgagcaagc tacgcagaat
     5161 acgatttttc agcaagttaa ataatccggt tttatccata aagtttgcgt attcttcaat
     5221 ggaatagttc gtatttttaa aatcaaagtc atacattgtt tccgaagtat taatcttaat
     5281 tgtgttttct cttttggcta aaagtaaag
//
LOCUS       DAOPCD010000037         5234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_13454_length_5234_cov_6.383665, whole genome shotgun sequence.
ACCESSION   DAOPCD010000037 DAOPCD010000000
VERSION     DAOPCD010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13454_length_5234_cov_6.383665"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..1240
                     /locus_tag="PLQ55_01770"
     CDS             197..1240
                     /locus_tag="PLQ55_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPK28696.1"
                     /translation="MAIMDIVKFSPSTQDWLVFKSPGTEFNKNSKLIVGPGQTAVCVY
                     NGKIVGEFENGTHRLDSANLPFIRETAKAIYGGVAPYQMEVYFFNKTVKLDFLWGLAN
                     GIQIQDPKFNVIIVINARGQMGLRLVNKQFFLTQLYGSFNTHLVPYTKVQDYFRGTIN
                     TKIKPVIMSYIKNHQVSALELPTIYENVAEEAISKLQPEFKTFGFELINLSVESLEPR
                     REDLEKVNELLHKRAEFDIVGDERYRTARTFDVLEGAAHNEGSGAAGIGMGLGVGLGA
                     GQAASQMFGSVADTGANKEVIKIVCPSCSHHVNEGTKFCPECGHRFVHNCPKCNSKVQ
                     PGSKFCPECGEKL"
     gene            1258..1728
                     /locus_tag="PLQ55_01775"
     CDS             1258..1728
                     /locus_tag="PLQ55_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28697.1"
                     /translation="MKNKKLLIINMLIALLFLVISILFIAFLEINKPLGLILFILMLV
                     ISFAGLGWFSYYASAKKLVPETFVMLSVPTYVFQFIPIIMFFLCSDYFTNGVLVMVPV
                     LFMLISVIIYVAITFSLIYYNQKAVESDEMTEAKSHKIEDEASYYNEDGSFKGL"
     gene            1747..2016
                     /locus_tag="PLQ55_01780"
     CDS             1747..2016
                     /locus_tag="PLQ55_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28698.1"
                     /translation="MMNCNSCGGSLTSSEQTCKYCGAPNPSYVGSYSAKRRICRSTTA
                     PSYTPPRTESRSTPAAKKSVNWFMFVLLLIFFWPAAIVYLIIRIK"
     gene            2753..3166
                     /locus_tag="PLQ55_01785"
     CDS             2753..3166
                     /locus_tag="PLQ55_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28699.1"
                     /translation="MYDKFKKHYLVLAKKPLSTTQKGILLGAGAVLAFAAFALPVAAV
                     GGISASAATTTGALALIGDMQIGVGMAGLLTFLVGGGLMGTTYKLLDSYNKREVMREF
                     RKLDYKEVAQLFSVVLLRIDHAQKNNVKFSVQRIC"
     gene            3982..5181
                     /locus_tag="PLQ55_01790"
     CDS             3982..5181
                     /locus_tag="PLQ55_01790"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MspI family type II restriction endonuclease"
                     /protein_id="HPK28700.1"
                     /translation="MKKENKIKNKSGQKAYLLIKEMINSLQHDLNAEIILSDKYSIGY
                     PEQEKQFKMDFKLSFPNINNETWLLKSTNSIRERIYGTEFFAHHIRKIDKNVSKIYVV
                     VPDSISEKELRNKDNYSKKITGGKYTSSITDVITVGELRKLIVEYCFQDLSQGIRSNI
                     LGADAEQEVLRSLNCRDNLELWNDYETNKHDLKSSNFDIFKEILTKSNLRMGIDVLDV
                     VRATDNIPLLVNGGKPKTDVAVMIKSSKFNIQRTISIKNTSSKKVTVHEGDVSDLIAA
                     LNISNDSRLAIALSKFQECGSELRLGELYPDMVEVLHSELKNYNVELTLFFLFGTNSP
                     LVVHPIQVADMILYVDKLYAENQESYVINYVSMFGDRGQFGTPFSWTYPSKRRGQRIQ
                     IKGFTNN"
BASE COUNT         1847 a          784 c          955 g         1648 t
ORIGIN      
        1 ttaattatta tttttatccc ctaaactttt ctagtgtata accctaaaga cgggaattta
       61 aacaaaatta tctacttaat caaaagtagt gactatcaag ttattaaagt gtctacattt
      121 agttgactag tgcagcttac tttgctatac taataatagt ttattattaa aatacttaat
      181 aagggagaaa agtgcgatgg caataatgga cattgttaaa tttagtcctt cgacacaaga
      241 ttggcttgtt tttaagtcac cgggaacaga atttaataaa aattcaaaat taattgttgg
      301 tcctggacaa acagcagttt gtgtttataa cgggaaaatt gttggggaat ttgaaaacgg
      361 tacgcaccgg ttagattcgg caaatttacc atttattcgt gaaacagcga aagcaattta
      421 tggtggcgta gcgccttacc aaatggaagt ttactttttt aataaaacag ttaaacttga
      481 ctttttatgg ggacttgcta atggcattca aattcaagat ccgaagttta atgtgattat
      541 tgtcatcaat gcccgcgggc aaatgggatt aagactcgtt aataaacaat ttttcttaac
      601 gcagttatat ggatcattta atacccactt agtgccctat acaaaagttc aagattactt
      661 ccgcggcaca attaatacaa aaattaaacc agtcattatg tcgtatatta aaaatcatca
      721 agttagtgcg ttagaattac cgacaattta tgaaaatgtt gccgaagaag caattagcaa
      781 actacagcca gaatttaaaa cgtttggttt tgaattaatt aatctttcgg ttgagtcact
      841 tgagccccgc cgtgaagatt tagaaaaagt taatgaatta cttcataaac gcgctgaatt
      901 cgatatcgtt ggtgacgaac gttatcgcac cgccaggacg tttgatgtac ttgaaggtgc
      961 tgcgcataat gaaggaagtg gagccgccgg tattggtatg ggactaggcg ttggccttgg
     1021 cgctggtcaa gcagcgtcgc aaatgtttgg cagtgtcgct gatacgggag cgaacaagga
     1081 agtcataaaa attgtttgcc caagctgttc acatcatgtt aacgaaggaa cgaagttttg
     1141 tcctgagtgt ggccatcgtt tcgtccataa ctgcccaaaa tgtaactcaa aagtgcaacc
     1201 aggtagtaaa ttttgtccag aatgcggcga aaaactttag aaaggtaaca atcaacaatg
     1261 aaaaacaaaa agctcttaat catcaatatg ctcattgcct tattatttct tgtcattagc
     1321 atacttttta tcgccttctt agaaattaat aaaccactag ggctgatttt atttatttta
     1381 atgctcgtca ttagttttgc cggcttaggc tggtttagtt attacgcaag cgccaaaaaa
     1441 cttgtcccgg aaacgtttgt catgcttagt gtaccgacgt atgttttcca gtttatcccg
     1501 attattatgt ttttcctttg tagtgactat ttcacaaatg gtgtgcttgt aatggtgcca
     1561 gttttattta tgcttattag tgtgattatt tacgttgcga ttacgttttc cttaatttat
     1621 tacaatcaaa aagccgtcga aagtgatgaa atgacggaag caaaaagtca taaaattgaa
     1681 gacgaagcat cttactataa tgaagatggt tcgtttaaag gtttataata gaaaggaacg
     1741 ttaattatga tgaattgtaa ctcatgtggt ggtagtttaa catcaagcga acaaacgtgt
     1801 aaatattgtg gcgctcctaa tccttcttac gttggctcct acagcgcaaa gcggcggata
     1861 tgtcgtagta caacagcgcc aagctatacg ccaccgcgca cagaatcacg ctcaactcca
     1921 gcggctaaaa aatcggttaa ttggtttatg tttgtccttt tattaatttt cttttggcca
     1981 gcggcaattg tttatttaat tattcgcatt aaataaaacg gcgttattta acgtttataa
     2041 agatcgggtt gatatttcag cccttttttt agcgcaagat gacgttcaaa aacgtttaaa
     2101 taaacgggaa agctagtttt ttgccgctta atttaatcaa aaaaattccg aatgtaaatg
     2161 gtttcataaa aaccttgaca atgtcaaggt ttattgaacg ataaaatgac gtttttctaa
     2221 gcatacgtgc gaagtgttgt tattttatta tgaaaactaa aattatgttg aattgaataa
     2281 tacgaattaa aaaatgagta acgataatat tcaaagagaa aaagaattat ttgttagttt
     2341 tcagtacctt cgattaatgt atgatttgga ctacataaca gagcaaatga aaaaaagcca
     2401 atactgaaat gctgattcaa ggggaggtgt aatccaaaaa tatacaacga atcccataaa
     2461 aaacattgca agagatgtaa gtgcgccaaa acaaaacaaa gatcgtgaac tggtgcttga
     2521 aagaattaat gtgtttagag agaaaaacaa aaataacaac ttcaatttat tttatgacaa
     2581 caagattgaa gaagtggtta aagaagtgtt taaatttgat aaatataatt ctcaattcta
     2641 tttatttagt atccaactcg ctattgataa tgagtatgaa tacatttatt gagaaggaag
     2701 tagtgaaaaa atctccctta ttctctataa aagcaagaac actttaaatg aaatgtatga
     2761 taaatttaaa aaacattatt tagtgttagc taaaaaacca ttatcaacta cacaaaaagg
     2821 catattgctt ggtgcaggtg ctgtgttagc atttgcagct tttgcacttc ctgtggcggc
     2881 agttggtgga attagtgctt cggcagcgac aacaacagga gctcttgcac ttatcggtga
     2941 tatgcaaatt ggagtaggaa tggctggact tcttacgttt ctagtagggg gcggattgat
     3001 gggtacgaca tataaactat tagactctta taataaacga gaagttatga gagaattcag
     3061 aaaactagat tacaaagaag tcgcacaatt gttctcagtg gttttattac gaattgatca
     3121 cgcacaaaaa aacaatgtca aatttagtgt tcaaagaata tgttagtgaa tatatgcaaa
     3181 ctgtagaaga tttcaagagc gatacgaact atgttttgtt tattgaaaga atgaacgttg
     3241 aattaaataa agaaaaaata aacacgttcc ataaatttga taaagaacta atgaaagtca
     3301 tagcagttta aataacaaat cattaaaaac agtgaaacag tatatatttt gttaaaatct
     3361 cgttgaaaaa gggagtgata atatgctcaa aaccaaaact gttatgcact acgagtgcca
     3421 gtcaggtttg aactcaaacg gcacttttca tgaaaaaaaa ggggctccat tttccttggt
     3481 ttacccaaac tcggcaaaaa ctaaccaaaa ctatgttttt agagcaaaaa aaccagtccc
     3541 tttaaacacc gtaatgactc caagcgtata ttgtgttagt aaggcgtaaa aaacggctaa
     3601 aacactgcaa aaaccgtcta aaatggcggt ttttctttaa ttttgatgat ttgatattta
     3661 aaataatcga cactttttgt aataattaca ccttatttct ttataaattt tgtttccgtt
     3721 acagtggttt gaactcaaaa accaatttcc cgttacagtg gttcgaaccg aattgccata
     3781 tttaaaaaac aatcatataa tcaactaaaa ttagttatga gattacacag aatatatact
     3841 tttttaacat aggaatgcaa tttgattatg gttttgtcac atgtgcagaa gggtaatttg
     3901 gagtgaaagt gccaaacaaa ccgcaccgtg ttataattaa tctatattta ggtttgtaaa
     3961 attgttaaaa taattaagaa aatgaaaaaa gaaaataaga ttaaaaacaa atctggacaa
     4021 aaagcgtact tattaattaa ggagatgatt aatagtcttc agcatgacct taatgctgaa
     4081 ataattttat cggataaata cagcattgga tatccagaac aagaaaaaca atttaaaatg
     4141 gatttcaaac tttcgtttcc taatattaat aatgaaacat ggcttttaaa gtcaactaac
     4201 tctattaggg aaaggatata tggtaccgag ttttttgcgc atcatattag aaaaatagat
     4261 aagaatgttt ctaaaatata tgttgtcgtt ccagattcaa ttagtgaaaa ggaactaaga
     4321 aacaaagata actactcaaa aaaaattact ggtggtaaat atacatcatc tattactgat
     4381 gtgataactg taggagaact tcgtaaatta attgttgaat attgttttca agatttatca
     4441 caaggaataa gatcgaatat tttgggggct gatgcggaac aagaagtgct tcgctcgtta
     4501 aactgccgtg acaacttaga actttggaat gattacgaaa caaataaaca tgatttaaaa
     4561 agttcgaatt ttgatatttt taaggaaata ctaacaaagt ctaatctaag aatgggcatt
     4621 gatgttttag atgttgtacg tgcaacagac aatataccat tactagttaa tggtggtaaa
     4681 ccaaaaacag atgttgcagt aatgattaaa tccagtaaat ttaatattca aaggactatt
     4741 agtattaaaa acacatcatc taaaaaagtc acagtacatg agggtgatgt atcggattta
     4801 attgctgcac ttaacatcag taatgattct aggttggcaa tcgcattgtc aaagtttcaa
     4861 gaatgtggta gtgaacttag actcggtgaa ctttatcctg atatggttga agttttacac
     4921 tctgaactta aaaactataa tgtagaacta actttgtttt tcctctttgg tacaaacagt
     4981 cctttagtag tccacccgat tcaagtagcg gatatgatac tttatgttga caaattatat
     5041 gccgagaacc aagaatcgta tgttattaat tacgtatcaa tgtttggtga tagagggcaa
     5101 tttggaactc ctttttcgtg gacataccca agtaaaagaa gaggtcaaag aatccagata
     5161 aaaggattta ctaacaacta acgttatttt aatatattat tatttagttt tttcggcatc
     5221 ccgaatagtt tttt
//
LOCUS       DAOPCD010000038         5185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_13600_length_5185_cov_3.311891, whole genome shotgun sequence.
ACCESSION   DAOPCD010000038 DAOPCD010000000
VERSION     DAOPCD010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13600_length_5185_cov_3.311891"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..147)
                     /locus_tag="PLQ55_01795"
     tRNA            complement(72..147)
                     /locus_tag="PLQ55_01795"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Ala,seq:tgc)
     gene            complement(156..232)
                     /locus_tag="PLQ55_01800"
     tRNA            complement(156..232)
                     /locus_tag="PLQ55_01800"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(196..198),aa:Pro,seq:tgg)
     gene            complement(479..871)
                     /locus_tag="PLQ55_01805"
     CDS             complement(479..871)
                     /locus_tag="PLQ55_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28701.1"
                     /translation="MLIYIQLLLDYYSKAFEGLFAFHEWPKALIDKYLIDNNVELTIP
                     NVPNIEFCLAGVGYGQVSVVIPGPDRCSEIKDVFEEQNFTVEHVVENNYYKVINLEGN
                     FEMMFNYYTEPKVTPNYPGFVNMVLYQP"
     gene            complement(1120..2319)
                     /locus_tag="PLQ55_01810"
     CDS             complement(1120..2319)
                     /locus_tag="PLQ55_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK28702.1"
                     /translation="MKITFMLFYYDPENPTINYLYRPLWDKLIKAGHEINIITPNPTR
                     GIPLSEQEKYKKTSVEKKGAFTIHRVKCYTYHPSRFSKFKLLRRYISVSRRLAKKLRK
                     VESDVVFVSSNPPLLYSYLVTRYCARKKIPVIYNVQDIYPDNIFKENSFAYKVFNPLQ
                     VKSLARASEVVTISETMRKTLLKKGDFNNKISVIYNFDISEVRKTPLVKKYTFFDPLQ
                     FNVVYAGNIGYVQDIDVILSAAKLLVHEPRIHIHIVGEGSQAARIAKRIHDENIVNAT
                     YYPPHSVEDSAQLYKNADVNVISILPGIIKTALPLKTASCLAAQKPIIFIGLETFYHN
                     EEWVNEVSELYPINYRNYEKVAETIEKLANAKTKPKLKYDQIELFDKEKNVNEYFNLI
                     TKYLKEE"
     gene            complement(2523..2720)
                     /locus_tag="PLQ55_01815"
     CDS             complement(2523..2720)
                     /locus_tag="PLQ55_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28703.1"
                     /translation="MTDLTQYIFMSNDEWWTYSEEYDCAVPTAKCPAKGIREFNRNRN
                     LITGFIMIGDGSVPPEYDPYI"
     gene            complement(2878..4017)
                     /locus_tag="PLQ55_01820"
     CDS             complement(2878..4017)
                     /locus_tag="PLQ55_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPK28704.1"
                     /translation="MIVLNLKLDNILAFKDFEVNFSYPRKLKKTLIEGEHLRTIPTFR
                     YKKLVVLIGSNATGKTSLIKLIWRISLFLHRKNPSFLDSLISENNEHGNIEIDFVNEY
                     DDVAILYRAKIRIIKNNVNNQNIVEVAMYSKKLTEGDSYENSINELDNHEYVFESWQV
                     ALQEFYDNFGWQVVLPSNEELFDKFYIHADINESEKDDYLDILNNVMKTLDPAIKNVF
                     HSKDAADAIVIEYSDERKVLIQNGNDISSIPLLSSGTKYGVAISNILFSIKNHRNGLY
                     LVDEQFSYVTADIETAILAKMTALLNSGEQLFFTTHNSNILELGFPFHSFYFMKKTII
                     DGAPIINVSCASELENRNNVSPKNMFDNDAFGTAPNVDGIYSIGK"
     gene            complement(4241..>5185)
                     /locus_tag="PLQ55_01825"
     CDS             complement(4241..>5185)
                     /locus_tag="PLQ55_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HPK28705.1"
                     /translation="DNFNNDAFGLINAEEDVKALLEAWNFKFKNYTGETFFRSAVLHQ
                     QVGPTYAFDKSKQEAVSDHYISTMVSSHESYRKDADKFFDKKIGLKLKVPDLNLKSDG
                     VVTYGVLKMKDYVANDGLFHRHMPFTFKSGLGTADSYVFAVREDPKKVAYYRGGNYEV
                     FKVNIRNTDLMIVLPNEEVNINHVNITEAYTNFVNEAELVPAFGYVPFFHNKVEGLNL
                     TDTLENVITHNEIYMSKLLKDDVYNDLVIDAVVQSNDFEFNKYGVAGESMTAVSGCTS
                     TGPLYDEPIDLSVNRPFYAFSLKDKYPIFINKVSKPGA"
BASE COUNT         1724 a          929 c          737 g         1795 t
ORIGIN      
        1 atttgaaccc ccgacctgct gggtatgagt cagccgctct aaaccaactg agctacgcag
       61 ccacaatata atggtggagc cgacgaggat cgaactcgct acctcatgcg tgcaaggcaa
      121 gcgctctccc agatgagcta cggccccata ataaatggtc gggatgacag gatttgaacc
      181 tgcgaccttc tgttcccaaa acagacgcgc taccaagcta cgctacatcc cgatgcttgt
      241 atatattacc aaaggtataa aaatttagca agtattaaca aacttgagtt cttgctttct
      301 tgcactaaca atcgaaaagg ttaatttatc tattaattga ttagcggtta aattatcttt
      361 ttttatcaaa tatgcatgtt tcttgaagat aaaaagatat ttaaaagact attactctct
      421 ttaaaatttg cgtttatgct tattaagaaa atggattata tcatcgcttc ttttattttt
      481 atggttggta taaaaccatg ttgacaaatc ctggataatt cggtgttact tttggttcag
      541 tataataatt aaacatcatc tcaaaattac cttcaagatt aatcacttta taatagttgt
      601 tctcaacaac atgttcaaca gtaaaatttt gttcttcgaa aacatctttt atttccgaac
      661 aacgatctgg accaggaata acaactgaaa cttgaccgta acctacgccc gcgagacaaa
      721 attcaatatt tggaacatta gggatagtta gctcaacatt attatcaatt aaatatttat
      781 caataagtgc cttaggccat tcgtgaaacg caaataagcc ttcaaaagct ttcgaatagt
      841 aatcaagcag taattgaata taaattagca tcccacctgt cgctagtctt tcattataag
      901 tggagtctaa aaagttatag cttatccgta tagtctcgtt agcgttaatg caaaaagtgc
      961 ggctaaaaat aaaaatttgg tctttttcct aatcactctc caaaaatttc ttacatacaa
     1021 tcgcatttta tattaattat gctgttcgtg caacaaataa tagaaaatgt attaaaggat
     1081 agcttcataa tgcttaaaaa agctttaata atcagttcac tattcttctt ttaagtactt
     1141 agtaattaag ttaaagtact cattaacatt cttttcttta tcaaaaagtt caatttgatc
     1201 atactttaat tttggcttag ttttagcatt agcgagtttt tcaattgttt ccgcaacctt
     1261 ttcataatta cgataattaa tgggatagag ctcacttacc tcatttaccc attcttcatt
     1321 atgataaaaa gtttcaagtc caataaaaat aatcggtttc tgtgccgcta gacaacttgc
     1381 cgttttaagt ggtaatgccg ttttaattat ccctggcaat attgatatta catttacatc
     1441 cgcattttta taaagctggg cactatcttc cacactatgt ggcgggtaat aagtagcgtt
     1501 aacaatattt tcgtcatgaa ttcgtttagc gattcttgca gcttgactac cttcaccaac
     1561 gatatgaata tgaatgcgcg gttcatgcac taaaagtttt gccgcactta aaatgacatc
     1621 aatatcttgg acatagccaa tgttaccagc gtaaacgaca ttaaattgca atggatcaaa
     1681 aaatgtatac ttcttcacta gcggagtctt ccgcacttca ctaatatcaa agttatagat
     1741 gacacttatt ttattattaa agtcgccttt ctttaaaagt gttttacgca ttgtttcact
     1801 aatagtgacg acttcactag cgcgcgctaa acttttaact tgtaaaggat taaaaacttt
     1861 atacgcaaaa ctattttctt taaagatatt atcaggataa atatcttgaa cattataaat
     1921 aactggtatt ttctttctcg cacaatacct tgttactaaa taactataaa gcagtggcgg
     1981 gttactagaa acaaagacaa catcactttc tactttacgc aattttttcg ctaaacggcg
     2041 cgacacactg atatagcgtc taagtaattt aaatttacta aagcgactcg ggtgataagt
     2101 gtaacacttt acacgatgaa tcgtaaacgc cccttttttc tcaacactcg ttttcttata
     2161 tttttcttgt tcacttaacg gaatcccgcg tgtcggatta ggggtgataa tattaatttc
     2221 atgccccgct ttaattagct tatcccaaag cggacgatat aagtaattaa tcgtcggatt
     2281 ctcggggtca taataaaaaa gcataaatgt tattttcata ctttaaagta taacacgaca
     2341 ttaataagaa aagtgaattt taagtatgat ttgccgcatt attcactaat aacacaaaat
     2401 ttatgcttta tttatataat attcgcgacg tgtttttcgg tcatagataa ttctagtttc
     2461 gcgttttatt tgttgcggag tgtaacctat tgactcacgc attttaacaa acttttcaat
     2521 acttatatat agggatcata ttctggtgga acactaccat caccaatcat aatgaaccca
     2581 gtaattaaat tacgattacg attaaattca cgaatacctt ttgccggaca tttcgcggtt
     2641 ggcacggcgc agtcatattc ttcactatat gtccaccatt catcattact cataaatata
     2701 tattgtgtta aatcagtcac ttttcattac tcctcgcttt tacctaatct tacactctta
     2761 caaataatag agtcaagtgt attcttatgc acatgaaact aaatttcttt tcaaaaatag
     2821 aaatgagaac tcaatattca tatttattgt ccagaatttt ggattgcaac atgtatttca
     2881 tttaccaata gaatagatac cgtcaacatt cggcgctgtt ccaaaagcat cattatcgaa
     2941 catattcttt ggtgatacat tattgcgatt ttctagttct gatgcacaag acacattgat
     3001 tattggtgca ccatctatga ttgtcttttt cataaaatag aacgaatgaa aaggaaaacc
     3061 taattcaaga atatttgaat tgtgtgtagt aaaaaatagt tgttcaccac tattaagtaa
     3121 agcagtcatt tttgctaaaa ttgctgtttc aatgtcagca gtcacatatg aaaattgctc
     3181 atccactaaa tagagtccgt tacggtgatt tttaattgaa aaaagaatat tactgatcgc
     3241 caccccatat ttagtaccgc ttgaaagcag cggaatactt gaaatgtcat taccgttttg
     3301 aattaatact tttcgctcat cactatattc aataactatc gcatcagcag catctttact
     3361 atgaaaaaca ttcttaattg ctggatctaa tgttttcatt acattgttta atatatctaa
     3421 ataatcatct ttttcacttt cattgatatc agcatgtata taaaacttat caaataattc
     3481 ttcatttgat ggaaggacta cttgccaacc aaaattatca taaaactctt gtaaagctac
     3541 ttgccaactt tcaaagacat attcatgatt atctaactca ttaatactat tttcgtatga
     3601 atcaccttca gtgagtttct tgctatacat tgcaacttca acaatgtttt gattatttac
     3661 attattttta attattctaa ttttagcgcg gtataaaata gcaacatcat catattcatt
     3721 tacaaagtca atttcaatgt tgccgtgttc attgttttca cttataagac tatctaaaaa
     3781 actaggattt ttacgatgca gaaataaaga aattctccaa attaatttaa tgagagaagt
     3841 tttccctgtt gcgtttgaac caattaacac tactaatttc ttatatctaa aagttggaat
     3901 tgtgcgaaga tgttctcctt caattagcgt tttcttaagt ttacgcggat aactaaaatt
     3961 aacttcgaaa tccttaaagg ctagaatatt atctaacttc aaatttaaga caatcatatt
     4021 atttgctcct tgtatgttca agttttttga acttacaatt ttaatgtaat aaattttaat
     4081 ttgaaaaaca aatattttca aactttttaa aactttttaa aaaaatgtta aatacttaaa
     4141 tagaatatgc agcgaccaat aaattaaaat caatttttaa aaaaagacta cattttgatg
     4201 tagccttatt tttagtaaat agttttatga caaacaataa ttatgcgcca ggctttgaca
     4261 ctttattaat aaagattggg tatttatctt ttaaagagaa ggcataaaac ggacgattaa
     4321 cagaaagatc aattggttca tcgtaaagtg gaccagttga tgtacagccg gaaactgcgg
     4381 tcattgattc tcctgccaca ccatatttat taaattcaaa gtcatttgat tgcacaacag
     4441 cgtcaataac taaatcatta taaacatcat ctttaagtaa tttactcata tagatttcgt
     4501 tatgcgtaat aacattttct aatgtgtctg ttaaatttag accttcaact ttgttatgga
     4561 agaagggtac atagccaaaa gcgggaacaa gttcagcctc attaacaaaa ttagtgtaag
     4621 cttcagtgat atttacatga ttaatattaa cctcttcgtt tggtaaaact atcattaaat
     4681 cagtatttcg aatatttact ttaaatacct cataattacc tccgcgataa taagcaactt
     4741 tctttggatc ttctcttaca gcaaagacat aggaatccgc tgtgcctagc ccgcttttaa
     4801 atgtaaaagg catatgtcga tgaaaaagcc catcgttagc gacataatct ttcattttta
     4861 aaacaccgta agtcactact ccatcacttt taagatttaa atcaggaact ttaagcttta
     4921 aaccaatctt cttatcaaaa aatttatcag cgtctttccg gtaagattcg tgcgaagaga
     4981 ccattgtcga aatgtaatga tcacttactg cttcttgttt actcttatca aaagcataag
     5041 ttggtccaac ttgttgatgt aagactgccg agcggaaaaa agtttcacct gtgtaatttt
     5101 taaatttaaa attccatgct tctaagagcg cttttacatc ttcttcggca ttaattaaac
     5161 caaaagcatc attattaaag ttatc
//
LOCUS       DAOPCD010000039         5180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_13610_length_5180_cov_3.490341, whole genome shotgun sequence.
ACCESSION   DAOPCD010000039 DAOPCD010000000
VERSION     DAOPCD010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13610_length_5180_cov_3.490341"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2004
                     /locus_tag="PLQ55_01830"
     CDS             <1..2004
                     /locus_tag="PLQ55_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPK28706.1"
                     /translation="SDIQEQFINKIANNNVYMVGDIKQSIYGFRNANCDIFREKYARY
                     RKNEGGKKIDLNDNYRSRREVLQSINAILSEIMTEEYGGANYKKEHVINSGNLKYDEF
                     KEERQKVGLEVINYVVPKKNKKNRDNNVLIRDKIDYELDYIINDIEHKIVNKYQVYEN
                     KVGMRDVRYGDFTILIAQGTKFNRIEERFIRAGIPVYVDRDEEANENNINIAINKLLT
                     IYVAYTKSDEPKGFEFRLAVASFLRGFVNKYSDQQLFDLLKDDNWDFSGDPILARIKK
                     LAQTTKLLPVTEIIERLITEFDVYGALYNIRDLTQNTMKLSSKLEEIENLAALGLDLE
                     AIVNYFKEREIAGVNAALKRVKLEHDAVTIMTIHRSKGLEFPIVYYMDLESNFYQVSR
                     SQVMKVCPKYGFILPEPSYKSQKTFIGFLSNIYTSKETLSEKIRLLYVALTRAKELMV
                     VIHSTNEDGEPYKTFEKSSNLKQLLLQSTYYDQKRLVVEPEYTQQRLSESEHSKEVKY
                     IYSLHEHFYKFNKKPKTYTVSEIVDVDERLLARGTLLHRYFEEYDFTTKDLNYISDER
                     DRQHMLNLLSLPFFANANANNIFREYEFIDDGTNKTYAIDCFIIQDDKIMLFDFKLSN
                     IYHPSYDVQVRNYAHYLQKVFKKSVEGYLISITDLKVRKVSNE"
     gene            1997..3085
                     /locus_tag="PLQ55_01835"
     CDS             1997..3085
                     /locus_tag="PLQ55_01835"
                     /inference="COORDINATES: protein motif:HMM:NF039499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HPK28707.1"
                     /translation="MNDKKTYTPISNENFLRLLRFYKIPESAEDEVLYNLYIETVELL
                     TLHHQTFENIPYINLDHQRLILQLIHDYDFRMRGLNFEERRSLLKDELFHNKLINVVV
                     DKYGSSAIFKYDSGTYLTPFSMEISTINVYLNFIMLKLGSIPRHNKATELYAELLTSA
                     FSYVLTITELLVRGFEKEALATWRSLHELEATLLLIQDEKVLAQYNQHILYALAFNKL
                     IAQAESDKVFIEIKAKMKDLKLKSKDTKRFIEYGWLLAHNDFDLNIHKFNFRDGVQTL
                     AGLNHKRDIYQIASEVTHSSPLTLFTKRHYFLSIALENLYSSFLTIEVLFAAFYIKNT
                     TKNEAEFYEVTRSIYLEDINFVKDRITK"
     gene            complement(3136..4794)
                     /locus_tag="PLQ55_01840"
     CDS             complement(3136..4794)
                     /locus_tag="PLQ55_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HPK28708.1"
                     /translation="MENKKTKTFTVVYNGKTTTHDEPVRILDIVGPDKNLICAYVNGR
                     VRELTFLLNRDAILETKTLDNGEGIRIYQTSLRFVAAMAMKRIRPELNVRFSYNVSRS
                     IFIQITNKGVTVDRLLVEKLQREMRNIVEADLPFNRTKITIEEAEKLYEELGYKDKLE
                     ILKYRPEPTVHIYECDGYENYMYGYMVPSSGYLKNFIIRPYSPGMLLQYPRSETKGEI
                     PEFKDAPMFGKALKASHNWAKLTNLDSVAHINHYIENGHIVELINIAEAKHYQMLSEL
                     GQRILEDSEDIRLICIAGPSSSGKTTFANRLRIELMSRGLNAIRLSIDDYYLPRELAP
                     RDEYGNYDLESIYALDIELFNKNMLDLINGEEVELPKFDFKKGQRVPGRKLKVAANQP
                     IIIEGIHALNEQLTSYIPKHQKYKIYIAPQAQINIDDHNPISLTDLRLLRRIVRDKKY
                     RNASAKETMAMWPSVRAGEFKWIYDTQEEADYVYNSLLSYELAVMKKHALPLLLEFDY
                     LSEFFPIAERLIRMLKYFLDMDEKWVPSNSLLREFIGGSCFEDV"
     gene            complement(4886..>5180)
                     /locus_tag="PLQ55_01845"
     CDS             complement(4886..>5180)
                     /locus_tag="PLQ55_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28709.1"
                     /translation="VRTNNYYTTDKKDNIKPDFKKYVTFTYNFNNATHHELINTKFFP
                     YFEKKGYVNYLVNEDTPLDTPTLSYGSSNKEISLPNDDTELETIISNYESYFS"
BASE COUNT         1759 a          804 c          877 g         1740 t
ORIGIN      
        1 agcgatattc aagaacaatt tataaataaa attgccaata ataatgttta tatggttggt
       61 gatataaaac agagtattta tggttttaga aacgctaatt gtgatatatt ccgtgaaaaa
      121 tatgctagat atcgcaaaaa cgaaggcggt aaaaagattg atttaaatga taattatcga
      181 agtcgtagag aagtcttaca atcaattaac gctattttaa gtgaaataat gactgaagaa
      241 tatgggggcg ctaactataa aaaggaacat gtgataaatt cgggcaatct taaatatgat
      301 gaattcaaag aagaaagaca aaaagtagga ttagaagtaa ttaattatgt tgtgcctaaa
      361 aaaaataaga aaaatcgtga taataatgtt ttaatccgtg acaagataga ttatgaactt
      421 gattacatca ttaatgacat cgaacataaa atagttaata aatatcaggt ttatgaaaat
      481 aaagtcggta tgcgtgatgt gcgttatggt gactttacaa ttttgattgc gcaaggaacg
      541 aaatttaatc gaatagaaga acgctttatc cgcgcaggaa ttcctgttta tgttgatcgt
      601 gatgaagaag caaatgaaaa taatattaat atcgcgataa ataaattact aactatttat
      661 gttgcttaca caaaaagtga tgagccgaaa ggttttgaat tccgtttagc ggtcgctagt
      721 ttcttacgcg gatttgttaa taaatatagc gaccaacaac tttttgattt attaaaagat
      781 gataattggg attttagtgg tgacccaata ctagcacgca ttaaaaagct agcacaaacc
      841 actaaactct tacccgtaac agaaattatt gagcgcctca ttactgaatt tgatgtatat
      901 ggtgcactat acaatattcg agatttaacg caaaatacta tgaaattgtc gagtaaatta
      961 gaagaaatag aaaatttagc ggcattaggt ttagatttag aagctattgt aaattacttt
     1021 aaagaacgtg agattgcggg agtcaatgct gccttaaaac gtgtaaaatt agagcatgat
     1081 gctgtaacga ttatgacaat ccaccgttca aagggtctag aatttccgat tgtctattat
     1141 atggaccttg aatctaattt ttatcaagta agtaggagtc aagtgatgaa ggtatgtcca
     1201 aagtacggtt ttattttacc cgaaccgtcc tataaatccc aaaagacttt cattggtttc
     1261 ttgtcaaata tatatacatc taaagaaaca ttatccgaga aaattagatt actttatgtt
     1321 gccctaacac gtgccaaaga gttgatggtt gtcattcatt ctactaatga agatggagaa
     1381 ccttataaaa cttttgaaaa gtctagtaac cttaagcaac tccttcttca atcaacttac
     1441 tatgatcaaa agcgtttagt agttgagcct gaatacacac aacagcgact tagcgagagt
     1501 gaacatagta aagaagttaa atacatttat tcgcttcatg aacattttta taaatttaat
     1561 aaaaagccta aaacttacac ggttagtgaa atagttgatg ttgatgagag gcttttagcg
     1621 cgtggtactt tgctccatcg ttattttgaa gaatatgatt ttactacgaa agatcttaat
     1681 tatattagtg atgaaagaga tcgacaacat atgcttaacc tgttaagttt acctttcttc
     1741 gctaatgcta atgcgaataa tatattccgt gaatatgaat ttattgatga tggaacaaat
     1801 aaaacctatg cgattgactg ctttatcatt caagatgata agataatgct ttttgatttt
     1861 aaactttcaa atatttatca tccaagttat gatgttcaag taagaaacta tgcacattat
     1921 ttacaaaagg tttttaagaa aagtgttgaa ggctatttaa ttagtattac tgacttaaaa
     1981 gtgaggaaag tatcaaatga atgataaaaa aacatataca ccgataagta atgaaaactt
     2041 tttgcggttg cttcggtttt ataaaattcc agaatcagcg gaagatgaag tcttatacaa
     2101 tttatacata gaaaccgttg aacttctcac attacatcat cagacttttg aaaatatacc
     2161 gtatattaat cttgatcatc agcgattaat attacaactt attcatgatt atgattttcg
     2221 gatgcgtggt cttaactttg aagaacgtcg gtcactactt aaagatgaac tttttcataa
     2281 caaactcatt aatgttgttg ttgataaata tgggagcagc gctattttta agtatgattc
     2341 tggcacttat ttaactccgt tttctatgga aatatcgaca attaatgttt atttaaattt
     2401 cataatgctt aaattaggat cgattccacg ccataataaa gcgacagaac tatatgcgga
     2461 attactgaca agtgcttttt cctatgtcct tacgattaca gaattactcg tgcgcggttt
     2521 tgaaaaagaa gcgcttgcta catggcgctc actgcatgaa ttagaagcaa cactactctt
     2581 aattcaagat gaaaaggtac tcgctcaata taatcaacat attctttacg ccttagcgtt
     2641 taataaactt atcgcacaag cagaaagcga taaagttttt attgaaatta aagcgaagat
     2701 gaaagactta aaacttaaaa gtaaagatac gaaaagattt attgaatacg gctggctttt
     2761 agcgcacaac gattttgatt taaacattca taagtttaat tttcgtgacg gggtgcagac
     2821 gctcgctgga ctaaatcata aacgcgatat ttatcaaatt gctagtgaag ttacacatag
     2881 ttcgccactt acattattta ctaaacgaca ttacttttta agtattgccc ttgaaaatct
     2941 atacagctca tttttaacaa ttgaagtgct ttttgcagcg ttttacatca aaaacaccac
     3001 aaaaaatgaa gcggaatttt atgaagttac gcgttctatt tatctagaag atattaactt
     3061 tgtaaaagat cgcataacaa aataaaaaag agccactcgg ttaaaagtgg ctcaagagtt
     3121 agcaattatt aatgattata catcttcaaa acagctgccg ccgataaatt cacggagtaa
     3181 tgaattagaa ggtacccact tttcatccat atcaaggaaa tatttaagca tccggattaa
     3241 gcgttccgca ataggaaaga attcacttaa ataatcaaat tcaagcaaga gcggtaacgc
     3301 atgctttttc ataaccgcta attcgtaaga gagcaatgaa ttatatacgt aatcggcttc
     3361 ttcttgtgtg tcataaatcc atttaaattc accagcgcga acacttggcc acattgccat
     3421 tgtttcttta gcactagcgt tacgatattt cttatcgcga acaatacgac gtaataatct
     3481 taaatcagta agactaattg ggttatgatc atcaatgtta atttgtgctt gtggtgcaat
     3541 ataaatttta tatttttggt gttttggaat ataactagtt aattgctcat taagtgcgtg
     3601 aattccttcg atgatgattg gttgattagc cgctactttt agttttcgtc ctggtacgcg
     3661 ttgacccttt ttaaagtcaa actttggtaa ttcaacttct tcaccattaa ttaagtcgag
     3721 catgttttta ttaaataatt cgatatcaag ggcataaatt gattctaaat catagttgcc
     3781 atattcatca cgcggtgcta attcgcgtgg taagtagtag tcgtcaattg ataaacgaat
     3841 agcgttaagt ccgcgactca ttagttcaat gcgtaatctg tttgcaaatg ttgtcttacc
     3901 agagctactt ggacctgcaa tacaaattaa gcgaatatct tcggaatctt ctaaaatacg
     3961 ttgtcctaat tcacttaaca tttggtaatg tttagcttcg gcgatattta ttaattcgac
     4021 gatatggcca ttttcaatat agtggttaat atgggcgaca ctatctaaat ttgttaattt
     4081 agcccagttg tgactagctt ttaatgcttt gccaaacatt ggtgcatctt taaattcagg
     4141 aatttcacct tttgtttcgc tacggggata ttgaagtagc attccaggtg aataaggcct
     4201 aattataaag ttttttaaat aacctgaaga aggaaccata tatccataca tatagttttc
     4261 atagccatca cattcatata tatgcacagt cggttctgga cgatatttca atatttcgag
     4321 tttatcttta tatccaagtt cttcatataa cttttctgct tcttctattg ttattttagt
     4381 gcgattaaat ggtaagtccg cttcaacaat attacgcatt tcccgttgca atttttcaac
     4441 aagcaatcga tcaacagtga cgcctttatt tgttatttga atgaagattg agcgcgacac
     4501 attatatgaa aaacgcacat ttagttcggg tctaattctt ttcattgcca tcgctgctac
     4561 aaagcgaagc gatgtttgat aaatgcgaat accttcgcca ttatctagtg tctttgtttc
     4621 aagtatcgca tctcgattca aaagaaatgt taattctcgg acgcgtccat tgacataagc
     4681 acaaattaag ttcttatccg gacctacaat atctaagata cgtactggct cgtcatgagt
     4741 tgtagttttt ccattgtaaa ctacagtaaa agtttttgtt tttttatttt ccatagattc
     4801 tccttttata taaagcttaa atagtaagtt aattatatac aacaacaagt ttttttccta
     4861 aagaaataac aatagcaatt attaattagc taaagtacga ttcgtaatta gatatgatcg
     4921 tttcaagttc ggtgtcgtca ttaggtaatg atatttcctt attactactc ccgtaactca
     4981 aagtaggtgt atctaaaggg gtgtcctcat ttactaagta atttacataa cctttctttt
     5041 caaagtaagg aaaaaacttt gtattaatta attcgtgatg agtagcgtta ttaaaattat
     5101 atgtaaatgt cacatacttt ttaaaatcag gtttaatgtt gtctttttta tctgttgtgt
     5161 aatagttatt tgtgcgaaca
//
LOCUS       DAOPCD010000040         5145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_13718_length_5145_cov_3.842043, whole genome shotgun sequence.
ACCESSION   DAOPCD010000040 DAOPCD010000000
VERSION     DAOPCD010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13718_length_5145_cov_3.842043"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1118)
                     /locus_tag="PLQ55_01850"
     CDS             complement(180..1118)
                     /locus_tag="PLQ55_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28710.1"
                     /translation="MKTRKLKIFALALTLPLLTGCKLIHNFICGIALNVAFNSPDLLS
                     KVNVVEGGAKLTPIEDKPTSADKRASLTYGIEALVLPKRIETVQYGYEIIIDFTFTFS
                     EGAQDYFYIRDVEDAQEGEYNVDGTILYPTGTVENPSNEQEISDWLTVWRMFDLKNQD
                     AKDITITLTGKSLNKTKSQTYYFNLNGDLIALPEQGDVDIDREYALVVEQAGVPKYLD
                     VVVPKEELVVGKNIPLKVMWVEFLLEEGTGTLTIDIETDLSADYDVTLPTENSYAVTV
                     NETLIDYPVNVRLKLGHPLPSEDLAIRFKAVVTSNN"
     gene            1538..1975
                     /locus_tag="PLQ55_01855"
     CDS             1538..1975
                     /locus_tag="PLQ55_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017755872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HPK28711.1"
                     /translation="MSKLHNVLNKQVANLAVFFVKLHHHHWYIKGQHFYGLHAKFEEF
                     YDEVNELYDAVAERLLMIGGKPYSTMKDYLANSSLVEASGGETATEMVTAIKQDFKTL
                     RDEFNALIKVAQDEGDEVTTDLLLGAVTSFDKHLWMLNATLGK"
     gene            2087..2162
                     /locus_tag="PLQ55_01860"
     tRNA            2087..2162
                     /locus_tag="PLQ55_01860"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2120..2122,aa:Val,seq:gac)
     gene            2280..3419
                     /locus_tag="PLQ55_01865"
     CDS             2280..3419
                     /locus_tag="PLQ55_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28712.1"
                     /translation="MIKIVPVYGGRKLRQFINFPVKLYKNSKQFTPYIFEDEISNLTP
                     SKNPASAWCDFKLFLAYRDRKIVGRICGIISHYANKKYDQKRVRFNRIDMIDDIEVTK
                     ALIKAVEEWGYSRGMNEIVGPLGYSDQDKEGLLVEGFEYSNMFATFYHFPYYMKHLEE
                     LGFVSDAVWVEHRIIIPKEIDERFERVSEMVKRKYNVKVRRLKSKRQIQPYVRKALAL
                     TNRAYDHLYGYVPMSEKLMDMLARQYVPLINLKYLILVVDEKDELLAFGLSIPSPVFA
                     LKKIKGKLFPFGFIRFLRALKKSKQLDLLLVAVEPELQNSGLANVIMYESMKSAIADG
                     IEYAETGPQLEYNKPVQRLWKHLNYFNHKRRICYVKPITEVEGEE"
     gene            3409..4677
                     /locus_tag="PLQ55_01870"
     CDS             3409..4677
                     /locus_tag="PLQ55_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPK28713.1"
                     /translation="MRNRKPKKQRIVKKRHQRYVNLANFLLGRLIRRSYGVKQAKIDI
                     KQYEPCLIVSNHILTFDPILMTSIFGHQIYYVASELIFSLGWKSRLLTHCFAPIPKTK
                     SMADVTTVKNIMQIVKEGGSVGVYVEGNATMTGGLSSMPAAIGKLVLRLKIPLVIFNF
                     EGGYLSLPRWATYTKREKHVRGKIRKVLYYEDYKDMTADEINELIIENININAYEESP
                     QYTYRGKRNAEGLRRLLFTCPICHGVNTAKTAYNNYSCAKCGEILTYDEHGYLHSTKW
                     DKPKNLIELDKANNLAYQEHIINNPNFSVSVPGNFTEVFRKRRKHFGPATLTLSREGL
                     TIKFKRKMKRHVTQHFTIDELDTMAMQQKEVLVIYPRHEQTKMLKIPKTEIVSSYQFV
                     VTLQILKNILQYPDAKPDEVPRLSAQDLGL"
     gene            4711..>5145
                     /locus_tag="PLQ55_01875"
     CDS             4711..>5145
                     /locus_tag="PLQ55_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28714.1"
                     /translation="MRTLFLANTWDFNPFWISIFQIGVVGALILIANTIRRKTPFLRN
                     YLVPTGLIAGFLGLGLKYLFQWLGVEIYGTPIVDESYMHFLTYHSLAIGFIALGLVTA
                     EKKVEKEGRALKSGALIVGGYLIQGIVGLILSLGVSLIFVNYA"
BASE COUNT         1721 a          811 c          935 g         1678 t
ORIGIN      
        1 aatctttttg atatatattt actatttaac ttgattaaga atagttaatc tccttgttat
       61 tatatgttca tatatatttt aaattgtgac gacaacataa aatcagcgta ttgttaaaaa
      121 aatcgcaaat aattgaaaaa taattttaac acttaaattt aattcactta actaaaactc
      181 tagttattac tagtaacaac cgccttaaat ctaatggcga gatcttcaga aggaagcgga
      241 tgaccaagtt ttaatctaac attaacggga taatcaataa gtgtttcatt tactgtaaca
      301 gcgtatgaat tttcagtggg taatgttaca tcataatcag cgcttaagtc tgtttcaata
      361 tcaattgtta atgttccagt gccttcttca agcaaaaatt caacccacat tactttaagc
      421 ggaatgtttt ttccgaccac taattcttct ttaggaacta caacatcaag atattttgga
      481 acaccagctt gctccacaac taaggcatac tcgcgatcaa tatcaacatc accttgttcc
      541 ggaagcgcga ttaagtcgcc atttaaatta aaataataag tttgagactt tgttttatta
      601 agtgacttac ctgttaaagt tatagtaata tcttttgcgt cttggttttt taaatcaaac
      661 attcgccata cggttagcca atcacttatt tcttgttcgt tactcggatt ttcaacagtg
      721 ccagttggat agagaatagt accatcaaca ttatattcgc cttcttgtgc gtcttcgaca
      781 tcacgaatat agaaataatc ttgcgctcct tcactgaaag taaaagtaaa gtcaatgatt
      841 atttcataac catattgaac agtttcgatt cgtttaggaa gaacgagtgc ttcgatacca
      901 taagttaaac tcgcgcgttt atctgcggaa gttggtttat cttcaattgg tgttaacttt
      961 gcaccacctt ctacaacatt aactttgctt aataaatctg gtgaattaaa agcgacattt
     1021 aaagcaatgc cacaaatgaa attatggatt aacttacacc cagttagtaa aggtagtgta
     1081 agtgcgagtg cgaatatttt aagtttacga gttttcataa caaatcctcc tgtatccatt
     1141 gtactttaaa acaagaaatc ttgttaggag tatttatatt gattaagtga taaaagagca
     1201 aaaaaaatct tacacaaggt gtgcaagatt atttgttatt gctgaagatt acttgatcaa
     1261 gcagtaactt cgctagttta tttaagaatt tacgcatact ttcacctcct taacgttgct
     1321 attcttttag gggggcggca agtaagttaa cttacttaca ggctcattat acaccaatta
     1381 taaatataag caataaaaac actcaaaccc cataaataaa acttataata tgtgataata
     1441 gcgattatat gttgatattt tttaaaggga gcatcatttt aggtaacatt ttcaaataat
     1501 ggtataatgt gtattgaaga aaaaaggagg aaaaattatg tcaaaattac ataacgtctt
     1561 aaataaacaa gttgcaaact tagctgtgtt ttttgttaag ttacatcacc accactggta
     1621 tattaaaggt caacattttt acggattaca tgctaaattc gaagaatttt atgatgaagt
     1681 taatgaactt tacgatgctg tagccgaacg tcttttaatg attggtggta aaccatattc
     1741 gacaatgaaa gattatctcg ctaatagttc gcttgttgaa gctagtggcg gtgaaactgc
     1801 tacggaaatg gtaacagcaa ttaaacaaga ctttaaaacc ttaagagatg aatttaatgc
     1861 actaattaaa gtagcacaag atgaaggtga tgaagtaaca actgacttac tacttggtgc
     1921 tgttacatca tttgataagc acctatggat gcttaacgct acattaggta agtaaatttt
     1981 agcgaaacaa ccccagttca atatatttca aactggggtt ttctttttta taaatcgcaa
     2041 ttatatgaat tgacaaagtt aatcgctttg ttatacttat gtggctggat gtttagctca
     2101 gctgggagag catctgtttg acgtacagaa ggtcgggggt tcgattccct cagcatccac
     2161 cattttttta aaaatagctc acgcaatcgt gagtttttta ttttcgttca ttattcataa
     2221 tatgttttac ttcaaatata atttgattat gttaaaatta tcaagtagag gaatgtatta
     2281 tgataaaaat agtccctgtg tacggcggaa gaaaacttag acaattcatc aacttccccg
     2341 tcaaattata taaaaattca aaacaattta ctccgtatat ttttgaagac gaaatatcaa
     2401 atttaactcc aagtaaaaat ccggcttcag cgtggtgcga ttttaaactg tttctagcat
     2461 atagagaccg gaaaattgtt ggtcgcattt gcggtattat tagtcattac gctaataaga
     2521 agtatgatca aaaacgtgtc cgatttaatc gaatcgatat gattgatgat attgaagtaa
     2581 caaaagcact cattaaagca gtggaagaat ggggttattc acgcggtatg aatgaaattg
     2641 ttggtccttt aggttatagt gaccaagata aagaaggact acttgtggaa gggtttgaat
     2701 attcaaatat gtttgccaca ttttatcact tcccgtatta catgaaacat ttagaggaat
     2761 taggatttgt aagtgatgct gtatgggttg agcaccgcat tattattccg aaagaaatcg
     2821 atgagcgttt tgaacgcgtt agtgaaatgg taaaacgcaa atataatgtc aaagttagaa
     2881 gacttaaatc aaaacgacaa attcaaccat atgtccgcaa agctttagcg ttaacaaata
     2941 gagcatacga ccatctttat ggttatgtac caatgagtga aaaactcatg gatatgctag
     3001 cgcgacaata tgtcccgctt attaacttaa aatatcttat tttagtagtg gatgaaaaag
     3061 atgaattatt agcgtttggg ctttcaattc cgtctcctgt ttttgccctt aaaaagatta
     3121 aaggaaaatt attcccgttt ggatttattc gctttttacg cgcacttaag aaatcaaaac
     3181 aattagactt actccttgtt gcggttgaac ctgaattgca gaatagtggt cttgctaatg
     3241 taataatgta tgaatcaatg aaaagcgcga ttgccgatgg gattgagtat gctgaaactg
     3301 gtccgcaact tgaatataat aaacctgtcc aacggctttg gaaacatcta aattacttta
     3361 atcataaacg ccggatatgt tatgtaaaac ctatcacgga ggttgaaggt gaggaataga
     3421 aaaccgaaga agcagcggat tgttaaaaaa cgtcatcaac gttatgtcaa tctcgctaac
     3481 ttcttactag gtagattaat tcgtcgttct tatggtgtta aacaagcaaa gattgatatt
     3541 aagcaatatg agccatgttt aattgtcagt aatcatatcc ttacttttga tccgatttta
     3601 atgacatcaa tttttggtca tcaaatatat tatgtcgcta gtgaattaat cttttcactt
     3661 ggttggaaat caagactttt aacgcactgt tttgccccta ttcctaagac taaaagcatg
     3721 gcagatgtaa caacggttaa aaacattatg caaatcgtta aagaaggtgg tagtgttggt
     3781 gtttatgttg aaggaaatgc gacgatgact gggggactaa gtagtatgcc agcggccatt
     3841 ggaaagttag tgttaagact aaaaattccg ctggtgattt tcaattttga aggtggatac
     3901 ttatcgcttc cacgctgggc aacttatacc aaaagagaaa aacatgtacg cggtaaaatt
     3961 cgcaaggttt tatattacga agattataaa gatatgacgg ctgatgagat taatgagctc
     4021 atcattgaga atataaatat taatgcttat gaagaaagtc cgcaatatac ttatcgcggt
     4081 aaacgcaacg ctgaagggtt gcgtcgttta ctatttacat gcccaatttg tcacggtgtg
     4141 aacactgcaa aaacggcata taataattat tcatgcgcaa aatgcgggga aatattaact
     4201 tatgatgaac atggatattt gcatagcacg aagtgggata aacccaaaaa tttaattgaa
     4261 ttagataaag caaataattt agcgtatcaa gaacatatca ttaataatcc taattttagt
     4321 gttagtgtcc ctggcaattt tacggaagtc tttagaaagc gccgtaaaca ctttggacct
     4381 gccacattaa cactttctcg tgaaggttta actataaaat ttaaacgtaa aatgaaacga
     4441 catgttactc agcattttac gattgacgaa ttagatacga tggcgatgca acaaaaagaa
     4501 gttttggtta tttacccgcg ccatgaacaa actaaaatgc taaagattcc aaaaaccgaa
     4561 attgttagtt cctatcagtt tgtagttacc ttacaaatct taaaaaatat cttacaatat
     4621 cctgatgcaa aacctgatga agttccgcgt ctttctgcac aggatttagg tctatagtcg
     4681 ttagtgtaaa aataaaggag gaaaagagat atgcgtacat tatttttagc taatacgtgg
     4741 gattttaatc cgttttggat ttcaatcttt caaatcggtg ttgttggtgc acttatttta
     4801 attgccaaca ctattagacg gaaaacacct ttcttacgta actacttagt gccaacaggt
     4861 ttaatcgctg ggtttcttgg tttaggttta aagtacttat tccaatggct tggtgtagaa
     4921 atttatggta ctccaattgt tgatgaaagt tatatgcatt ttctaactta tcatagttta
     4981 gcaattggat ttattgcttt agggttagta actgcggaaa agaaagttga aaaagaaggt
     5041 agagccctaa aaagcggggc attaattgtt ggcggatatc tgatccaagg tattgttgga
     5101 cttatcttga gcttgggtgt tagtttaatc tttgttaatt acgct
//
LOCUS       DAOPCD010000041         5020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_14115_length_5020_cov_3.775831, whole genome shotgun sequence.
ACCESSION   DAOPCD010000041 DAOPCD010000000
VERSION     DAOPCD010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14115_length_5020_cov_3.775831"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="PLQ55_01880"
     CDS             complement(<1..1343)
                     /locus_tag="PLQ55_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPK28715.1"
                     /translation="MLKQIKTQREAMKKNFPLSYEKRRELLINLQKAVKLYEKDIYEA
                     LYKDLHKDEWEVFVTELGIVYEEMHFAIKNLKKWMRRKKIKSFLVQFPAKNFLVPEPY
                     GQVLIMSPWNYPLQLTLLPLIGAIAAGNYAVVKPSAYAPEIAKVMQQIVATAFPNKEA
                     ILFLGGRKVNEEILQGVYDFIFFTGSPGVGQTVMRKASENLTPVVLELGGKSPLIIAE
                     DADIPLTIRRLKFAKLINCGQTCVAPDYVLLDKKLIDKVDDIRQAFSVEGEEKARLPK
                     IINEKHYLRLKGVLTEVGSDNFDEETQTINPTVVLNPPLDSELMREEIFGPILPIITY
                     ETLDEAIAFINERPKPLALYLFTKSKEIMNKVLRETTSGSVAINDAVVQLANPHFPFG
                     GVGNSGMGSYHGYHTFKTFSHMKAVLHKSRAFDFRFRYKPGPKTVGIFKKLTRNR"
     gene            complement(1327..2541)
                     /locus_tag="PLQ55_01885"
     CDS             complement(1327..2541)
                     /locus_tag="PLQ55_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPK28716.1"
                     /translation="MSKLGDRIFQKVLRTFSPILPWREPEILKAYDELVDVLIKQNKS
                     RIFIVMDEGLVQLKLHQPLLDALKNKQLNYRVFSDITANPTTAQVEEGLLAYIIYGGD
                     AIIGFGGGSPMDVAKAIAARVARPRKNLKQLGGLLKVRKKTPLLIAVPTTSGTGSEAT
                     VASIVVDERTKHKYAINDPVLIPSYALLVPTLTFKLPKFVTATTGMDALTHAIEAYTN
                     KGGRKFPNEKAINATRLVFENLKVAYDNPSDLEARKNMQLAAYDAGVAFTRNYVGYVH
                     ALSHPLSAYYGLAHGYVNALILPFMLEKYGRRIYRKLARLARLSGVVEEGLSNGETAK
                     LFIAKIRALNTYFEIPTTIPEIKEEDIPQLAVYANKEARPLYPTPRIYKTSELEEFYR
                     ELKGEIFNVETN"
     gene            complement(2705..3160)
                     /locus_tag="PLQ55_01890"
     CDS             complement(2705..3160)
                     /locus_tag="PLQ55_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28717.1"
                     /translation="MSKKRRIKELEAQVAALEAKVEGKEGQQTPKKAAKNSVNVYFDS
                     NFVNSLVSNQMKYGRRSRRDDLNVDPADKKAVRRARARRFFRFLWRLIVVLVIAALVA
                     AVVAAILMVIVWALVNFNIMTPTSNPFVGMVWEWIKWFFALFGMEVPIF"
     gene            complement(3179..4978)
                     /locus_tag="PLQ55_01895"
     CDS             complement(3179..4978)
                     /locus_tag="PLQ55_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28718.1"
                     /translation="MKIKTRLDFIVIDGTELVVNEALKTLAKLPVSDLVEFLKGPKLY
                     SRRDHANILKNVMRDEVWKFKLVSRVAEKGLSNDFFSRLDYFEDSPVTVLEGLFERMV
                     KIQPELMDYYLEDLWLFAIQVVSKDKELAKLYKKAQKSSTSPVVLKELFPTLEGLFGD
                     EEGDIDGVLARTFVEKSLHSATIKEIREIAEKNDVLLPTNITKQDIVDGIIAVLPKVA
                     KAKDVPHLTEDVKDMTVAQLKEFVNKHKLDVPTELKKQDVGELLVKNYVNKNAANVVS
                     DLQYELPERVEEATDFSDENPKVIRLRERVKELEKELEERPVENETKVDESELLKEQE
                     KVEALSKELKQTKAELSEKPKEVVVEVPGPTVIRTVISKKELQKQKEHYEAIIAEQEK
                     ERSMLISRTEYDEVLAEYGRVKALLDEKDIEKLEEKPVEPKPAPQQVPQYEEPYYGSL
                     HAKVDRLTDKVHELQLEILRKELANQKATPAPQEEQPETEVNIYFDSNLAKRMSDNKN
                     AIDVGKAVKGKKRKKKSAGRTIFWIIFILLLLAIVVFMGAWTLEHFNITPLAGPPWLA
                     DRFNAAVLWLHNAVQSVFDLFKGLFGKVRVLGR"
BASE COUNT         1387 a         1028 c          854 g         1751 t
ORIGIN      
        1 tatctatttc tcgttaattt cttaaaaatt ccaactgttt ttggaccagg tttatatcta
       61 aaacgaaaat caaaagcacg ggacttatgt aaaacagcct tcatatgact aaatgtttta
      121 aaagtatgat acccgtgata actccccatc ccgctattgc caacaccacc aaaggggaag
      181 tgcggatttg ctagttgcac aaccgcatca ttaatcgcga cagaaccact tgttgtctct
      241 cttagaactt tattcattat ttctttcgat ttagtgaaaa gataaagcgc tagcggttta
      301 ggacgttcat taataaaagc aatcgcttca tcaagtgttt cataagtaat aatcggtaaa
      361 attggaccaa atatttcttc acgcattagt tcgctatcaa gtggtggatt taacacaact
      421 gttgggttaa tagtttgtgt ttcttcatca aaattatcac tacctacttc agttaaaaca
      481 cctttaagtc gcaagtaatg cttttcatta ataattttag gtaaccttgc tttttcttca
      541 ccttcaacac taaatgcttg acgaatatca tcaactttat caataagttt tttatcaagt
      601 aacacatagt caggcgctac acatgtttgt ccgcaattaa taagtttagc aaatttaagg
      661 cgccgaatcg ttaaggggat atcagcgtct tctgcaatta ttaacggtga ctttccgcct
      721 aattcaagga cgactggtgt taaattttca cttgctttac gcatcaccgt ttgtccgact
      781 ccaggactac cagtaaagaa aatgaaatca tacacacctt gtaaaatttc ttcatttact
      841 ttacgaccac caagaaataa aatagcttct ttatttggaa aagcagtcgc aacaatttgc
      901 tgcattactt tcgcaatttc tggtgcgtaa gcacttggtt ttacaaccgc ataattgcct
      961 gccgcaatcg ctccaatcag tggtaaaagt gttagttgta atgggtagtt ccatggactc
     1021 ataattaaga cttgtccgta gggctcaggt acaagaaaat ttttagcggg aaattgcaca
     1081 agaaatgact taatcttttt tcttctcatc cattttttta gattttttat ggcaaaatgc
     1141 atttcttcat acacaatacc aagttcagtg acaaatactt cccattcatc tttatggaga
     1201 tctttgtaga gggcttcata tatgtctttt tcatataatt taactgcttt ttgcaggttt
     1261 attaatagtt ctcgccgttt ttcataactt aagggaaagt tttttttcat tgcttctctt
     1321 tgcgttttaa tttgtttcaa cattaaatat ttctcctttt aattctcgat aaaattcttc
     1381 taactcactc gtcttataaa tacgcggagt gggatagagg gggcgcgctt ctttgttagc
     1441 atacactgct agttgcggaa tgtcttcttc tttaatttct ggaatcgttg tcggtatttc
     1501 aaagtaagta tttaaagcac ggattttcgc tataaaaagt ttagctgttt caccgttact
     1561 taatccttct tcaacaaccc cactcagccg cgcgagtcgc gctaattttc ggtaaattct
     1621 tcgtccatat ttctctagca taaacggtaa tattaaagcg ttaacatagc catgcgctaa
     1681 cccgtaatat gcactaagcg gatgacttaa ggcatgaaca taaccaacat aattacgcgt
     1741 aaaagcaacc ccagcatcat aagctgctag ttgcatgttt tttcgtgcct caaggtcact
     1801 aggattatca tatgcgactt tgagattttc aaacactaat cgtgtagcgt taatcgcttt
     1861 ttcatttgga aatttacgac cacccttatt tgtgtaggct tcaatagcgt gtgttaaggc
     1921 atccatccct gtcgtcgctg tcacaaattt tggtaattta aatgtgagtg taggcactaa
     1981 taatgcataa cttggaatta aaactgggtc attaatcgca tatttgtgct ttgttctctc
     2041 gtcaacaaca atcgacgcta ctgtcgcttc acttcctgtt cctgaagttg tgggaacagc
     2101 aattaaaagc ggtgttttct tccgtacttt taagagcccg cctaattgtt ttaaattctt
     2161 ccgtggtcgc gctacacggg cggcaatggc tttagcaaca tccattggac tgccgccacc
     2221 aaaaccaata atagcgtcac caccataaat aatgtaagca agtagtcctt cttcaacttg
     2281 ggctgtagtc ggattagcgg ttatatcact aaatacacga taatttagtt gtttattttt
     2341 aagggcgtca agtaagggtt gatgtaattt aagttggact aaaccttcat ccattacaat
     2401 aaaaattcgg cttttatttt gcttaattaa aacatcaact aattcatcat acgcctttaa
     2461 aatttctggt tcacgccatg gtaaaatcgg gctaaaggta cgcaatactt tttgaaatat
     2521 acggtcgcct aattttgaca tcgcatgcac tcctttccgc tatagtgttt ttgtaatgat
     2581 attttttaat tgaacttatg ggtttaattt acttataaca atatttgtga ttcttctcat
     2641 ttttattata gcaaaaaagg gacttggggt ccctttgatt tgcttttatc taaatggtgt
     2701 caatctagaa gattggtact tccatcccga agagggcaaa gaaccactta atccactccc
     2761 agaccatgcc gacaaatgga tttgatgttg gggtcataat attaaagtta acaagtgccc
     2821 agacgataac cattaatatc gctgcgacga ccgctgcgac taaagcggca ataacaagca
     2881 ctacaattaa tctccataag aaacggaaga aacgacgagc gcgtgctctt ctgacggcct
     2941 ttttatcagc gggatcaaca tttaaatcat cacgacgtga acgccgtcca tacttcattt
     3001 gatttgatac taggctatta acaaagttac tatcaaaata gacatttacg gagtttttag
     3061 cggctttctt tggcgtttgt tggccttctt tgccttcaac ctttgcttcg agcgctgcta
     3121 cttgagcttc taattcttta attctacgtt ttttactcat aactcaaatc tctcctttct
     3181 atcgtcctaa tacccggact ttaccaaata gtcctttaaa taaatcaaag acactttgga
     3241 ccgcgttgtg gagccataat accgcagcgt tgaaacgatc agcgagccat ggtggtcctg
     3301 caagaggggt aatattgaaa tgctctaatg tccatgcacc catgaacacg actattgcta
     3361 agagcaagag aataaagata atccagaata ttgtccgtcc agcactcttc tttttacgtt
     3421 ttttaccttt aactgctttg ccaacatcaa tcgcgttttt attgtccgac atccgtttag
     3481 caaggttact atcaaaataa atattaactt ctgtttcggg ttgttcttct tgcggtgccg
     3541 gagtagcttt ttgattagct aattctttac gtaagatttc tagttgcaat tcatgaactt
     3601 tatccgttaa gcgatcaact ttagcgtgaa gtgaaccgta atatggttct tcatattgcg
     3661 gcacttgttg cggtgcaggc tttggttcaa ctggtttttc ttcaagcttc tcaatgtctt
     3721 tttcatcaag taatgcttta acacgaccat attccgctaa aacttcatcg tattcagtac
     3781 ggctaataag catggaccgt tctttttctt gttcagcaat aattgcttcg tagtgttctt
     3841 tttgcttttg taattctttt ttgctaatta ctgtccgaat tactgttggt cctggcactt
     3901 caacgacgac ttcttttggt ttttctgaaa gctcagcctt tgtttgcttt aattctttag
     3961 aaagtgcttc aactttttct tgttctttta aaagttcgct ttcatcaacc tttgtttcat
     4021 tttcgacagg tctttcttct aattctttct cgagttcctt aacgcgttcg cgcaacctga
     4081 taacttttgg attttcatca ctaaaatcag tagcttcttc gacacgttct ggtaattcat
     4141 attgtaaatc actaacgaca tttgccgcat ttttgtttac atagttttta acgagtaatt
     4201 caccaacatc ttgtttttta agttcagttg gaacatcaag tttatgcttg ttcacaaatt
     4261 ctttcagttg tgcaacagtc atatccttaa catcttccgt taaatgcggg acatcttttg
     4321 ctttcgctac ttttggtaaa acagcaataa ttccgtccac tatgtcctgt tttgtgatat
     4381 ttgttggtag taaaacatca tttttttcgg caatttcacg gatttcttta atcgtggcac
     4441 tgtgtaagct cttttccaca aatgtgcgcg ctaatacacc gtcgatatcg ccttcttcat
     4501 caccgaaaag cccttcaagt gtcgggaata attccttaag aacaactggg cttgtgctac
     4561 ttttttgtgc tttcttataa agcttagcaa gttctttgtc cttgcttaca acttgaatgg
     4621 caaagagcca taagtcttca agataataat ccattagttc gggttgaatc ttgaccatcc
     4681 gttcaaataa tccttcaaga acggtgacag ggctatcttc aaaataatca agtcgactaa
     4741 agaaatcatt tgaaagtcct ttctccgcaa cgcgtgagac aagtttaaac ttccagactt
     4801 catcacgcat aacattcttt aaaatgtttg catgatctcg acgtgaatat aatttagggc
     4861 cctttaaaaa ttcgactaag tcgcttactg gtaattttgc cagtgttttg agagcttcat
     4921 taacaacgag ctcggttcca tcaattacga tgaaatctaa tctggtttta attttcatgt
     4981 tgttttcccc tttgctaaca ttatatcatt gaaaagtgat
//
LOCUS       DAOPCD010000042         4986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_14222_length_4986_cov_3.141148, whole genome shotgun sequence.
ACCESSION   DAOPCD010000042 DAOPCD010000000
VERSION     DAOPCD010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14222_length_4986_cov_3.141148"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..325)
                     /locus_tag="PLQ55_01900"
     CDS             complement(14..325)
                     /locus_tag="PLQ55_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28719.1"
                     /translation="MKELKYNNQLVKVNDGTTGFDLLKILNIEGRSNVLVYKLNGELF
                     DLATPVEQDGEFSFVTKEDEEAFATLNHDAAHLMAQAILSIYPGANIGTNPKFSDRIN
                     T"
     gene            complement(589..1431)
                     /locus_tag="PLQ55_01905"
     CDS             complement(589..1431)
                     /locus_tag="PLQ55_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPK28720.1"
                     /translation="MFIIALDIGGTNIRAAIADQNGKLLEVVKEKTLRHDKEIFITQV
                     KGIITQLDLSKYDPIAISIGVPGRVRQNGFIDELPNIGITNIDLVSALQKTFKLPVSV
                     RNDAEMAAFAEAALGAGKELKSTYFVTISTGIGGCLIVDRTIKNPSPEIGHTLVPYRG
                     NFYELEKIASGDGVINLAALNEIYYANAQAFFKDVTYKVPYALKIFDEWLDLITDFFI
                     FVNEAFTPDVIAVTGGVLKSKKLFWDKFIERVPNANIQIAHFSEDAGLVGAASYGFKV
                     FASQ"
     gene            complement(1431..2876)
                     /gene="pyk"
                     /locus_tag="PLQ55_01910"
     CDS             complement(1431..2876)
                     /gene="pyk"
                     /locus_tag="PLQ55_01910"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPK28721.1"
                     /translation="MLNISKKTKIVATIGPVTNTLEKVRELIDAGMNVMRINFSHGTY
                     ETHKTIIDISKKLIEEHIYIPIMLDTAGPEIRCHFFEDGKVEIVKDSIVTINMKEVLG
                     TATEFSVNYPKLVDDILVGDMIKIDDGKLRLDVIEVDREKRLIKVKAFNTHVISNRKG
                     INIPGERMTLPALSERDKKDIKFGIENGIDMIAASFTRCAADVIQLKNYLAELGAPEI
                     PIIAKIENKQGVDNIKEILEVADGIMVARGDMGVDIPPEVVPVMQSDIILECRKVGKP
                     VIVATQMLDSMQTNPLPTRAEVSDVATAIKEGADAVMLSAETASGLYPVESAAMQQRI
                     AITMEEFLPYTQLANFGYNYSKKKRSDAIATSVASTANLIDAKAIVCFSETGKSAICI
                     SKSRPKCPIIMVTSSPAAGMRVAPYFGIYSIVVKTLPQLIEDMEAFALIKMRQLGLKP
                     NDNIIITGGTPTGTGSTNFLRIVALNKLGDI"
     gene            complement(2891..3853)
                     /locus_tag="PLQ55_01915"
     CDS             complement(2891..3853)
                     /locus_tag="PLQ55_01915"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPK28722.1"
                     /translation="MTVKVAVLTSGGDAPGMNAAIRAVIRSGLLAGYEMFAIFEGFRG
                     IIENKIVSVDRTFVSEIIMRGGTILKTARFPEFRDSKVVEKAAENLRFYGITHLIVIG
                     GDGSYRGAYALSKHGIKIIGIPATIDNDVMGTEQTIGFDTALNTITEAVDKLRDTSSS
                     HHRCSIIEVMGNRCGDLALYSGTACGVDLIISPEYRPSEEEIIETLKNINLNRARHAL
                     VIVSEKQFEDLEKLRERIVEETGFDAKIEVLGRIQRGGSPTASDRVLAGRLGVYAIDL
                     IAKGIDNVCVGLVDGRLRHHAIAEVTKSTNPNLDELLNVIDKLK"
     gene            4054..>4986
                     /locus_tag="PLQ55_01920"
     CDS             4054..>4986
                     /locus_tag="PLQ55_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28723.1"
                     /translation="MRLIKSNDFYSVKVRGYVTSLHLEVLDILYQPIMGFAATALYRY
                     LFTMRDFRKGDPLSFTLIHDHFGFSYDQISIAFSKLEGLGLMRSFYVEYNEFSEYVLE
                     LYAPKDPAAFSQDQIFMNLLNDVLGPRHTQDLLSLFTLDRETLNKEEVTSTFAEIYAD
                     RIFDIKGDDLYKGEPLENVVGNIKFEFDRAAFVNILTRERKILPSALSAEELKKAEQM
                     AGLYNLTEVATAERVGDFYDALQPFGARVDFVAMNKHLQELVKYPVLSKPLRRPVQEL
                     AGESQKIKRINLMETYSPVEFLQYLNEGTKIAPAD"
BASE COUNT         1488 a         1002 c          878 g         1618 t
ORIGIN      
        1 ggcacaagca attttaagta tttatcctgt cgctaaattt gggattggtc ccgatattag
       61 cgccaggata aatacttaaa attgcttgtg ccatgaggtg ggcagcgtca tgatttaaag
      121 tcgcaaaagc ttcttcgtcc tcttttgtca caaaggaaaa ttcaccatct tgctcaactg
      181 gagttgcaag gtcaaaaagt tcaccgttta gtttataaac taaaacatta cttctccctt
      241 cgatgtttaa aattttaagt aagtcaaaac ctgttgtgcc gtcattaact tttactagtt
      301 gattgttata tttgagttct ttcatatacc ctccttttta ataaaaaaac gcccatttaa
      361 ggacgctata tttgcgcggt accaccttaa ttcatgttgc catgcactct gttagtcgta
      421 acgtgactag cgactaactc atcgttagta ggctcgaaag tggtaagatt aatgctccct
      481 cctagtttgc accacccact aggtctctat aagtgaagtc attaagctct tgtctttgtc
      541 ttcgcctaaa taattttaca catatttgcc taaataggca agaagtagtt actgtgaggc
      601 aaatacttta aaaccgtaac ttgccgcacc gactaatccc gcatcttcac taaaatgcgc
      661 gatttgaata ttagcgttag ggacacgttc gataaatttg tcccaaaata attttttcga
      721 ttttaatact ccgcccgtaa cagcaatcac atcaggcgta aaagcttcat taacaaaaat
      781 gaagaagtca gtaatcaaat ctaaccattc atcaaagatt tttaaggcat aaggcacttt
      841 ataagtgaca tctttgaaaa aagcttgggc atttgcgtaa tatatctcat ttagcgctgc
      901 aagattaatt actccgtcac ctgaagcaat cttttctaat tcataaaaat taccgcgata
      961 aggcactagt gtatgcccga tttctggact ggggttttta attgtgcggt caacaattag
     1021 gcagccgcca atgccagtgc taatagtgac aaaataagtg ctttttaatt ctttacccgc
     1081 tcctaatgct gcttcagcaa aagcagccat ttctgcatca ttacggactg aaacagggag
     1141 cttaaaagtc ttttgcaaag cactaactaa atcgatatta gtaatgccaa tatttggtaa
     1201 ttcatcaata aatccgtttt gtctaacgcg acccggtaca ccaatggaaa tagcaatagg
     1261 atcatattta cttaaatcaa gttgtgtaat aatcccctta acttgcgtga taaaaatttc
     1321 tttatcatgg cgcagtgttt tttcttttac aacctcaagt aatttcccgt tttggtcagc
     1381 gattgctgcg cgaatatttg tgccaccaat atcaagtgca ataataaaca ttaaatatct
     1441 cctaatttat taagggcgac aatccgtaag aagtttgtcg aaccagtgcc agttggcgta
     1501 ccaccagtaa tgatgatatt atcatttggt tttaacccaa gttgtcgcat tttaatgagc
     1561 gcaaaagctt ccatatcttc aattaattgt ggtagtgttt ttaccacgat actataaatg
     1621 ccaaagtaag gtgcaactcg cattcccgcc gcgggactac tcgtgaccat aatgatcgga
     1681 cacttaggac gtgatttaga tatgcaaatc gcacttttac ccgtttcact aaaacaaaca
     1741 atcgctttag cgtcgattaa attcgccgtt gaggcgacgc ttgtggcaat cgcgtcactg
     1801 cgctttttct ttgaatagtt atagccaaag tttgcaagtt gagtatatgg taaaaattct
     1861 tccatagtaa tggcaatccg ttgttgcatt gctgcacttt caaccggata taaaccgcta
     1921 gccgtttccg cactaagcat cacagcgtca gcgccttctt taatggcagt cgcgacgtca
     1981 cttacttccg cccttgttgg gagcggatta gtttgcattg agtcaagcat ttgtgtagca
     2041 acgataactg gtttgccgac cttacgacat tctaaaatga tatcgctttg catcacagga
     2101 acgacttcag gtggaatgtc aactcccata tcaccacggg caaccatgat cccatcagcg
     2161 acttctaaga tttctttaat attatcaaca ccttgtttgt tttcaatttt agcgataatc
     2221 ggaatttccg gtgcacctag ttccgctaaa taatttttaa gttgaataac atcagcagca
     2281 cacctagtaa aacttgccgc aatcatgtca atgccgtttt caattccaaa tttaatatct
     2341 tttttatcgc gttcacttag tgctggtaaa gtcattcgtt caccaggaat attaatgcct
     2401 ttacgattgg aaataacatg cgtattaaat gctttaactt tgattaaacg cttttctcta
     2461 tcgacttcaa taacatcaag tcttaacttg ccatcatcaa tcttaatcat gtcaccgacg
     2521 agaatatcat cgactaattt aggataatta acactaaatt ctgtagcggt gcctaatact
     2581 tctttcatat tgatggtgac gatggagtct ttgacaattt caactttccc atcttcaaag
     2641 aagtgacaac gaatttctgg tccagctgta tcaagcataa tcggaatata aatatgttct
     2701 tcgattagtt ttttggaaat atcaataatt gttttgtgtg tttcataagt gccgtgtgag
     2761 aagttgatgc gcataacatt catcccggca tcaattaatt ctcgaacttt ttcaagagta
     2821 ttcgtcactg gtccaattgt ggcgacaatt ttagttttct tggaaatatt taacataaag
     2881 gtgttccttt ctatttgagt ttatcaatta catttagtaa ttcatctaaa tttggattag
     2941 ttgatttagt aacttcagcg atggcgtgat gtcgtaagcg cccatcaact aagccgacac
     3001 aaacattgtc aataccctta gcaattaaat caatcgcata tacaccaagt cgaccggcaa
     3061 gtacccgatc actagccgtc ggtgagccgc cgcgttgaat gcgccctaac acttcaattt
     3121 tagcgtcaaa gcctgtttct tcaacgatac gctcccgcaa tttttctaaa tcttcaaatt
     3181 gtttttcact aacgatcaca agcgcgtgtc gtgcgcgatt taagttaatg ttttttagtg
     3241 tttcaataat ttcttcttcg ctaggtctat attcggggga aataattaaa tcaaccccac
     3301 atgccgttcc gctataaagc gctaagtcgc cacagcgatt acccataact tcaataattg
     3361 aacagcggtg atggctgcta gaagtatcac gaagtttgtc aacagcttcc gtaattgtat
     3421 taagcgctgt atcaaaacca attgtttgtt cagtacccat tacatcatta tcaattgtcg
     3481 caggaatccc gataatttta atgccgtgct tacttaaagc atatgcacca cgataactac
     3541 cgtcaccgcc aataacaatt agatgagtaa taccatagaa gcgtaagttt tccgctgctt
     3601 tttcgacaac ttttgaatca cgaaactcgg gaaaacgcgc agtttttaaa atagtgccac
     3661 cgcgcataat aatttcacta acgaaagtac ggtcgacact aactatttta ttttcgataa
     3721 ttccgcgaaa gccttcaaaa atagcaaaca tctcatagcc agcaaggagt ccgctacgga
     3781 taactgcccg aatcgcggca ttcatccctg gagcgtcgcc acctgatgtt aatactgcta
     3841 ctttaactgt catattgctt cctccataca ttttatattt tatcatgtta acaatacctt
     3901 tgacttattt ataatagtca attttaaaga aagtgcgaat attttctttc tattctacaa
     3961 aaaagtgtta actttggtaa ttatttgttc tttgtaaaaa aatacatact taccttaacg
     4021 gtgcgttatt cattgtgtta taataatttt actatgcgat taattaaaag taatgatttc
     4081 tatagtgtga aagttcgcgg ttatgtaaca tcgttacatc ttgaagtttt agatatttta
     4141 tatcaaccaa tcatgggctt tgccgcgaca gccttatatc gctatttatt tacaatgcgt
     4201 gattttagaa aaggtgatcc gctatcgttt actttaattc atgatcattt tgggtttagt
     4261 tatgaccaaa ttagtatcgc ctttagtaaa ttagaaggtc ttggtttaat gcggtcattt
     4321 tatgttgaat ataatgaatt tagtgaatat gtcttagaat tatatgcacc aaaagaccca
     4381 gcagcgttta gtcaagatca aatctttatg aatttattaa atgatgtgct tggtccgcgc
     4441 catacacaag acttattatc tttatttacg ctcgatcgtg aaacattaaa taaagaagaa
     4501 gttacatcaa cttttgccga aatttacgct gatcgtattt tcgatattaa aggtgatgac
     4561 ctatataagg gtgaaccatt agaaaatgtt gtcggtaata ttaaatttga atttgatcgc
     4621 gctgcttttg ttaatatctt aacgcgtgaa cgtaaaattt tgccctcagc actaagtgct
     4681 gaagagctta aaaaagctga gcaaatggct ggtttatata atttgacgga agtagcaaca
     4741 gcggagcgtg tcggtgattt ttatgacgct ctacaaccct ttggcgctag agtcgatttt
     4801 gtggcgatga ataagcactt gcaagaactt gtgaagtacc ccgttttaag taaaccatta
     4861 cgccgtccag ttcaagaact tgcgggtgaa agtcaaaaaa ttaagcggat taacttaatg
     4921 gaaacatata gtcccgttga atttttacaa tacttaaatg aagggacgaa aattgctcct
     4981 gctgat
//
LOCUS       DAOPCD010000043         4906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_14460_length_4906_cov_3.563801, whole genome shotgun sequence.
ACCESSION   DAOPCD010000043 DAOPCD010000000
VERSION     DAOPCD010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14460_length_4906_cov_3.563801"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1587
                     /locus_tag="PLQ55_01925"
     CDS             271..1587
                     /locus_tag="PLQ55_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28724.1"
                     /translation="MQKKRTSAVLTLILAILMLTFFGLAHIVSTVYLVDGAPIGVLGM
                     LVKFKEVLLSLFVPNFGDLMSLAGFVATVVVLGVALVLALVWAIKLLYYRRRGWNLLS
                     PLLFLIAIFLGVLLVGSHTDVDLAFRQPTAQVATAYFFVVGVLMVSLSLHLVVVGLKT
                     AGTKKVASLLEEDLDDEYVVIDEQVEERVVEEKEEEPVVEEKVEETEVVEEAEEKVEE
                     EEELIVELDLEDPKLEALIRKLANEEIDKRPVPEKEVVVREVEKVVEVKPEPKPEPKP
                     EPKPEPKPEPKPVVVAKPKAEAKPKIERVSFPDRMQMVEDSAKANYNLLKNYLLSYGL
                     NSRVSNVADSFRLGRVLYARLTNSGNSGLKLYLPLDLEDYKDSKIPLKSAEGIKQYED
                     VPVFLYVRSDLSVKRAQELIDDVMLKHGIARKHDMKEVDHVKELVK"
     gene            1732..2736
                     /locus_tag="PLQ55_01930"
     CDS             1732..2736
                     /locus_tag="PLQ55_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28725.1"
                     /translation="MKLKKFMLAIGVIGLLLTGCDDPNTSSSQTSGSETSEITEAERF
                     SAAGYSPLKGWPVESIRMHYTREGIDAAWVFTNVLTPTGFGQNTDLYVRSYEDELGID
                     VFKSFEVVRVTADATTFTNYVALYDGKTDYTVIVDEEHEFGIEAFSGKVRIGVSFVEE
                     NEAVPSATYFRFTVQEKEEFPGAPKIDRSAHRSEITFSNDFKVTTRSKEIVEWAFSDL
                     YKFKVEVGESSTNVGNIPNNSGVGYLTPQLRIYKDQVLTLNMEQGTMKQVIFHGVNYT
                     EDGVDVKSIDAFPVVEGAQQFKTANAVLYHFDADNTTSLSLVVTANARLLDVTVIYFL
                     "
     gene            2844..3410
                     /locus_tag="PLQ55_01935"
     CDS             2844..3410
                     /locus_tag="PLQ55_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPK28726.1"
                     /translation="MQLLKPELNSLIQKEALDLFYKNGYQATLMRDLAAAVGMSVGNT
                     YRYYKSKHDLFTTLVQPVHEKLVELLEATHDNPEAHFNELCLTTIMTRFTAICSKYHK
                     ESVIFLEYYLAQEDDPVIQNFRGLIYRTVKAHLPQITMPMFDLLYHFIATGTVFVLRK
                     SAPENIVSNLKLLYVFLFKDVKDRINAL"
     gene            3407..>4906
                     /locus_tag="PLQ55_01940"
     CDS             3407..>4906
                     /locus_tag="PLQ55_01940"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPK28727.1"
                     /translation="MKKIFNFIVRFKVIFFGFWIALTIASIFMIPHVKINYDTTKYLP
                     EDTRVKESLRVMEEEFGITGQASVMIEKVETLQEVFVYKDEIKRIPTVMEVVWIDSFV
                     ERDTINDIQRIAEENGMSGVFDFANIPGLNAFYKDKSALLQIVFTEEDHSRAVGKAIE
                     QIRSYLEGIKRPYAMRGTAVNAYYTQKLTESEVLRVTVIVVPIILFILLVFTRSWAEP
                     IIFLIAVGVAVLVNMGTNLFFPSVSFLTNSTASLLQLAISMDYSIFLLHAYQKERAEG
                     LDKKDAMVMAMRKSFVSINSSMLTTAAGFLALMFMRYTIGIDLAAVMIKGILLSIIST
                     FTFMPGLILLTDKIVEKTKHKRFFPLLGGLTKWIVKLRYVVPAILLLVIVPSYLAQTN
                     NSFVYGEAAMSVSEGTPAAIEKNRIEAKFGKENLMVILVPRDEADAYIAQEKAMIKQL
                     YREFERANQDFVVQITSLSNLSDIETYIPNYDDLDQTIKNFLDDIFADIM"
BASE COUNT         1544 a          817 c          985 g         1560 t
ORIGIN      
        1 caaatccaga attatataat ggtaatgttg ctgattgtgc agaagtgtta cgtctagcaa
       61 tagtactaag caaaaagagc ctaaatttgt atgaggtgct ttcaatctta ggtccaaagg
      121 aagttgaaaa acgtcttaca gcaatgcaaa ataagctaaa atagctgatt tttcaacaaa
      181 atatatcatt tttgcatact ttctattgca atttgcgtgt gtttttatta ttatgaaagt
      241 aattaaaatt aatgaaagga caattttatt atgcagaaaa aacgcactag tgcagtactt
      301 actcttattc ttgcaatctt aatgttaacc tttttcggat tagcgcatat tgtttcgaca
      361 gtatacttgg ttgatggtgc cccgattggt gttttaggaa tgttagtgaa atttaaagaa
      421 gtactattat cattatttgt tccaaacttt ggtgacctta tgtctttagc gggctttgtc
      481 gctacagtag tcgtccttgg tgttgctctt gttttagcac ttgtttgggc gattaaatta
      541 ctttactatc gtcgtcgtgg ttggaactta ttatcgccac tcctattcct tattgctatc
      601 tttttaggag tattattagt tggaagtcac accgatgttg accttgcatt ccgtcaacca
      661 acagcgcaag ttgcaactgc ttacttcttt gtcgttggcg tcttaatggt tagtctttca
      721 ctccacttag tagttgtcgg tcttaaaact gctgggacga agaaagttgc ttcattatta
      781 gaagaagact tagacgatga atatgttgta attgatgaac aagttgaaga acgcgttgtt
      841 gaagaaaaag aagaagaacc agttgtcgaa gaaaaagttg aagaaactga agtagtcgaa
      901 gaagctgaag aaaaagtcga agaggaagaa gaacttattg ttgagcttga tttagaagat
      961 ccgaaactcg aagctttaat tcgtaaatta gcgaatgaag aaatcgacaa gcgtccagtt
     1021 ccagaaaaag aagttgtggt gcgcgaagtt gaaaaggttg tggaagttaa acctgaacca
     1081 aaaccagagc cgaaacctga accaaagcca gaaccgaaac cagagccaaa accggtcgtc
     1141 gttgctaagc caaaagctga agcaaaaccg aaaattgaac gtgtttcctt cccagatcgg
     1201 atgcaaatgg ttgaagattc cgcaaaagct aactataact tacttaaaaa ctatttatta
     1261 agttatggct taaattcacg tgtttctaat gtcgcggatt cattccgctt aggtcgtgtc
     1321 ctatatgcac gcttaacaaa ctctggaaac agtggactta aactttactt accacttgat
     1381 ttagaagact ataaagatag taagattccg cttaaatccg ctgaaggtat taagcaatat
     1441 gaagatgtac cagtcttctt atatgtccgc agtgacttat cagttaaacg tgctcaagaa
     1501 ttaattgatg atgtaatgct taaacacggc attgcccgta agcatgacat gaaagaagtt
     1561 gaccacgtta aagaactagt taaataaaat taattagtaa ataaacataa attagcctat
     1621 gcttgcatag gcttttttaa tataaagtgt aataatgtat ccgctttctt actctatcat
     1681 ttaaaatatt aaatgaagta tgataatata acttgatagc ggaggttatg tatgaaactc
     1741 aagaaattta tgttagcaat tggagtaatt ggcttactct taacaggatg tgatgatcct
     1801 aatacatcga gcagtcaaac ttctggtagt gaaacaagcg aaattacaga agctgaaaga
     1861 tttagcgctg ctggttattc accgcttaag ggctggccag ttgaatcaat aagaatgcac
     1921 tacactcgtg aagggataga tgcagcgtgg gtattcacga atgttttaac accaacagga
     1981 tttggtcaaa atactgatct ttatgttcgc agttatgaag atgaacttgg gattgatgtt
     2041 tttaaatcat ttgaagtagt gcgcgtcact gctgatgcca caacttttac taattatgtt
     2101 gcgctctatg atggtaaaac cgattacaca gttatcgttg atgaagaaca cgaatttggt
     2161 attgaagctt tcagcggtaa agtacggatt ggtgttagtt ttgtagaaga aaatgaagct
     2221 gttccgagcg ctacttattt ccgctttact gttcaagaaa aagaagaatt ccctggcgca
     2281 ccaaaaattg accgtagtgc gcatcgctct gaaatcactt tctctaatga ttttaaagta
     2341 acaacaagat caaaagaaat tgttgaatgg gcatttagcg atctttacaa gtttaaagtt
     2401 gaggttggtg aatcaagtac aaatgttgga aatattccga ataatagtgg tgtcggctac
     2461 ttaaccccac aacttcgtat ttataaagat caagtcttaa ctttaaatat ggaacaagga
     2521 acaatgaaac aagttatttt ccatggtgtt aattatacag aagatggtgt agatgttaag
     2581 tcaattgacg ctttcccagt tgtcgaaggt gctcaacaat ttaaaacggc taacgctgtt
     2641 ttatatcact ttgacgctga taacactact agtctttcac ttgtcgttac ggctaatgca
     2701 agactactcg atgttactgt tatttatttc ttataaaata ttaattattt acattataaa
     2761 gcccatattt gatgggcttt ttattttttt cttgcaacga ttttagcatt gctatacaat
     2821 attagtgaac aggtgtttac attatgcaac ttttaaaacc agaacttaat tcattaatcc
     2881 aaaaagaggc gcttgacctc ttttacaaga acgggtatca agcaacttta atgcgtgatt
     2941 tagcagcggc agtggggatg agtgttggga acacttatcg ctactacaaa agcaaacatg
     3001 acttatttac tacgcttgta caaccagtac atgaaaagct tgttgagtta cttgaagcaa
     3061 cgcacgataa tccggaagcg catttcaatg aactttgttt aacgacgatt atgacgcggt
     3121 tcaccgcgat atgttctaag tatcacaagg agtcggttat ttttcttgaa tactatttag
     3181 cgcaagaaga tgatccagtt attcaaaact ttcgtggtct tatttaccgc acggtaaaag
     3241 cacacttgcc gcagattaca atgccaatgt ttgacctact ttatcacttt atcgcgactg
     3301 gcactgtttt tgttttgcga aaatctgctc cagagaatat tgtatctaat ttaaaattac
     3361 tttatgtgtt cttatttaaa gatgtcaaag ataggattaa tgcattatga aaaaaatatt
     3421 taatttcatc gtccgcttca aagtaatctt tttcggtttc tggatagcgt taacaatcgc
     3481 tagtattttt atgattcccc atgtcaaaat aaattacgac acgacaaaat atttgccaga
     3541 agatacgcgt gttaaagaat ctttacgtgt gatggaagaa gagtttggca ttactggtca
     3601 agctagtgta atgattgaaa aagttgaaac attacaagaa gtgttcgtgt ataaagatga
     3661 aattaaaaga attccaacgg tgatggaagt tgtgtggatt gatagtttcg tcgaacgcga
     3721 tacgattaat gatattcaac gcattgctga agaaaatggg atgtccggtg tctttgactt
     3781 cgcaaatatt ccggggctta acgcttttta taaagataaa agtgctttgc tgcaaattgt
     3841 gtttacggaa gaagatcata gtcgcgctgt cggcaaggcc attgaacaaa tccgctccta
     3901 tcttgagggt atcaagcgac cttatgcaat gcgcggaaca gcagttaatg cttattacac
     3961 gcagaagtta actgaatcgg aagtattgcg ggtaacggta attgttgtcc caatcatctt
     4021 atttatttta ttagtgttta ccagaagttg ggccgagcct attatctttt taattgccgt
     4081 cggggtcgct gttttggtaa atatgggtac aaacttattt ttcccgtctg tatccttttt
     4141 aacgaattcg acagcctcac tcttgcaatt agcaatctcg atggattatt ccatattcct
     4201 cttgcatgct tatcaaaaag aaagggcaga gggacttgat aaaaaagatg caatggtgat
     4261 ggcaatgcgc aagtcatttg tgtcaattaa ctcgagcatg ttaacaactg cagccggatt
     4321 tttagcactg atgtttatgc gttacacaat cgggattgac ctcgcggcgg tgatgattaa
     4381 agggattttg cttagtatta tctcaacctt tacctttatg cctggtttaa tcttgctcac
     4441 tgataaaatt gtggaaaaaa caaaacataa acgctttttc ccacttcttg gtggactcac
     4501 taaatggatt gtaaaattac gctatgttgt gcctgcgata cttttactcg taattgtgcc
     4561 ttcttactta gcacaaacta ataatagttt tgtgtatggt gaagcagcga tgagtgtgag
     4621 cgaaggcaca ccagcggcaa ttgagaaaaa tcggattgaa gcgaagtttg gtaaagaaaa
     4681 cttaatggtt attcttgtac cacgcgatga agcagatgct tatattgccc aagaaaaagc
     4741 gatgattaaa cagctttatc gcgagtttga gcgcgctaat caagattttg ttgtacaaat
     4801 tacgagtctt tcaaacttaa gtgatattga gacttatatt cctaactacg atgatttaga
     4861 tcaaacgatt aaaaatttcc ttgatgatat ttttgccgat attatg
//
LOCUS       DAOPCD010000044         4903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_14470_length_4903_cov_3.935231, whole genome shotgun sequence.
ACCESSION   DAOPCD010000044 DAOPCD010000000
VERSION     DAOPCD010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14470_length_4903_cov_3.935231"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2374
                     /locus_tag="PLQ55_01945"
     CDS             <1..2374
                     /locus_tag="PLQ55_01945"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPK28728.1"
                     /translation="FDGDQMAVHVPLSEAAQEEALNLMLASKNILGPKDGKPIAAPSQ
                     DMVIGNYFLTVEESREDFVRRAEEARALGDEFGAQKFMQYAESEGKVFGSIDELMVAY
                     QTNSVHLHNRIALPGRAFNKTLFTPEMNKSYLVTSVGKVIFNTIFPNDFPYLNEPNKG
                     SLQMDKIEYFVPAGTNIREHIASLPLRKPINQEFLGLIINEVFTRYGTVKTSAILDKI
                     KDQGFHYSTKAGLTVSLHDITPIEGKEELVEAGEVEVRKINKAAARGFYSEKERYNAV
                     INVWKGVKDDVEAKLKEQFPLDKRNPLYIMFDSGARGKTTNFLQLKGLRGLMASPRGG
                     EIEIPIKSNFREGISVSEFFIASHGSRKGGADTAIKTADSGYLTRRLVDVAQNVIVTE
                     EDCGSDHGFVVTEIRIRENDTPAVPLSDRIAGRYTVHDIIHPTTGEVIIEANTLINKD
                     AALYIEKAGVKEVEIRSLFGCEAPSGICAHCYGTNLATGERVKVGEAVGIMAAQSIGE
                     PGTQLTMRTFHSGGVAQVEDVTQGLPRVQELFEARNPKGKAIITEIAGEVVSITQEED
                     RRYTVTVKNDLEEKSYLTEYGQQLRVKEGDTLKQGDRITEGAVHPRELLAVSTITAVQ
                     NYLLNEVQKVYRISASVRISDKHLEIIINQMLNRVYIIDGGDTSLLPGQRITTHQFTA
                     ANKEALINGLHPAVATPLILGIKAAALASDSFLSAASFQETTRILTDATVKGKLDPLH
                     GLKENVITGRLIPAGRGILTKEEQEALLEDFSVADTLDKIKASYDNSSNS"
     gene            2515..4110
                     /locus_tag="PLQ55_01950"
     CDS             2515..4110
                     /locus_tag="PLQ55_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28729.1"
                     /translation="MKKRLLLLPLLAMLLGGCNGGSSATPSISTPESIPSSEEPSVES
                     SAESVSVSIDTTSYEIATTTNYSNRELDHSIDMTALDSLGTQKMLVVPVVFSDTAHRA
                     TEEVHTMINKAFFGASEETGWESVASYYHKTSFGKLTLTGEVLPYFYSQWSTHAFGAL
                     EVSPTQEYGEGYYWDQTHHLIEEIYNTLDAELFLEYDQDGDGHVDAFWMVYIAEIKAL
                     GGEDDPFWAYKFYWNRYANTEKPTPNTYAWASYKFADEGVGYNFNIPDAHTYIHETGH
                     MFGLPDYYDYDSKTAPAGALDMMDHNVADHNMYSKYRLNWSKPFVVTGNADIHLRPAE
                     STGDFILIKDGWNGHVYDEYILIEYYTPTGLNEKDSQGRGYETKSSNVSTKSFDINGV
                     RMWHVDSRLMTYVFDPESLELTNAFWTDEIVVGDPFSYTIIGPSNTTSRSSTPEGDSK
                     VNKLLHLMDATGTTSRVGNWLRTPTTAGSTALFQTGDVVAADGWKKYIQTSATFNDGT
                     AVGYSVEIGEMDDNGVQIIIRKA"
     gene            4174..>4903
                     /locus_tag="PLQ55_01955"
     CDS             4174..>4903
                     /locus_tag="PLQ55_01955"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HPK28730.1"
                     /translation="MKCKVKRNTKKVTLLLSLLLLSSCNLEVTFFPYTHHISDTDTYT
                     RIETSDYYKVGAFARSYQDVLKSNLNGNSRVVPLKSSGEQKILVLPVNFPDYDLNELD
                     GNNGKTAHIHIQNAFFGENHTTRFYSVAGYYEASSYGKLKLSGDVAPWYTLPAEYSVS
                     AIKSRVRSNSDKLNETAEILNLALKEYAKSNDLSAFDLDENGVLDSAYVIYSYPFEPK
                     DVNNIFWAFAANMNKKDPELTHQAN"
BASE COUNT         1583 a          945 c         1048 g         1327 t
ORIGIN      
        1 ttttgacggt gaccaaatgg ccgtccacgt cccgcttagt gaagctgcgc aagaagaagc
       61 gctaaactta atgcttgcga gcaaaaacat tttagggcca aaagatggta aaccaattgc
      121 cgccccgtcg caagacatgg tgattggtaa ctacttctta accgttgaag agtcacgcga
      181 agactttgtg cgtcgcgccg aagaagcacg agcgttaggc gacgaatttg gtgcgcaaaa
      241 gtttatgcaa tatgctgaaa gcgaagggaa agtatttggt tcgattgatg aattaatggt
      301 tgcttatcaa acaaatagtg tccacttaca taaccgtatc gcgcttcctg gccgagcgtt
      361 taacaaaaca ttatttacac cagaaatgaa taagtcttat cttgtgacaa gtgttggtaa
      421 agtcattttt aatacaattt tccccaatga cttcccatac ttaaacgaac caaataaagg
      481 ctcattacaa atggataaaa ttgaatactt tgtgcccgct ggtacaaata ttcgcgaaca
      541 cattgcaagt ttaccgctgc gtaaacctat taaccaagaa ttcttaggtt taattattaa
      601 tgaagtgttt acgcgttatg gcacggtgaa aactagtgca attcttgaca aaattaaaga
      661 ccaaggtttc cattattcaa caaaagctgg tcttactgtt tcactccacg acattacccc
      721 aattgaaggt aaagaagaac tcgttgaagc tggtgaagtt gaagtgcgga aaattaataa
      781 agccgcagcg cgtggattct atagtgaaaa agaacgctat aatgccgtca ttaatgtctg
      841 gaaaggtgtt aaagatgacg ttgaagctaa acttaaagaa cagttcccac ttgataaacg
      901 taatccgctc tacatcatgt ttgatagcgg agcaagaggt aaaacaacaa acttcttaca
      961 acttaaaggg ttacggggct taatggcttc gccgcgtggt ggcgaaattg aaattccgat
     1021 taaatcaaac ttccgtgaag gcattagcgt gagcgaattc ttcattgctt cgcacggttc
     1081 acggaaaggt ggtgcggata ccgcgattaa aactgctgac tcagggtact taacccggcg
     1141 gttagtcgac gttgcccaaa atgttatcgt caccgaagaa gactgtggta gtgatcacgg
     1201 ctttgtcgtt accgaaattc gtattcgtga aaatgacacc ccagcagtgc cacttagtga
     1261 ccgtattgcg ggtcgctata cagtccatga catcattcac ccgacaactg gtgaagtaat
     1321 tattgaagcg aatactctta ttaataaaga cgctgcgctt tatattgaaa aagcgggtgt
     1381 taaagaagtt gaaattcgtt cgctctttgg atgtgaagcg ccaagcggaa tttgtgcgca
     1441 ctgttacggc acaaacttag cgactggtga acgtgttaaa gttggtgaag cggtcggaat
     1501 tatggcggca caatcaattg gtgaaccagg aacacaatta acaatgagaa cgttccacag
     1561 cggtggtgtt gcgcaagttg aagacgtcac ccaagggtta ccacgggttc aagaattatt
     1621 tgaagcccgt aatccaaaag gtaaagcaat aatcaccgaa attgctggtg aagttgtaag
     1681 tatcacgcaa gaagaagacc gtcgctatac cgttactgtt aaaaacgatt tagaagaaaa
     1741 gagttattta acagagtatg gtcaacaatt acgtgttaaa gaaggcgaca cacttaaaca
     1801 aggggaccgc attactgaag gtgctgtcca cccgcgtgaa cttctcgctg ttagcacaat
     1861 taccgcggtg caaaactatt tacttaacga agtacaaaaa gtttaccgca tcagtgcttc
     1921 tgtccgcatt agtgataagc acttagaaat tattattaac caaatgctca accgcgttta
     1981 tattattgat ggtggtgata cgagtctctt accaggacaa agaattacga cccatcaatt
     2041 taccgcggcg aataaagaag cacttattaa cggactccat ccagctgtag cgacaccatt
     2101 aattcttggt attaaagctg ctgcgcttgc aagtgactcc ttcttaagtg ccgcaagttt
     2161 ccaagaaaca acacgcatct taactgacgc cactgttaaa ggtaaacttg acccactcca
     2221 cggcttaaag gaaaacgtca ttactggacg cttaattcct gctggtcgcg gtatcttaac
     2281 gaaagaagag caagaggcct tgcttgaaga ctttagtgtc gctgacactt tagacaaaat
     2341 taaagcaagt tacgacaata gtagtaacag ctaaataaat taagggagca atataaaaac
     2401 atattgctct cttttagtaa ccggtttcat ataaatatat gaaccttatc atttaactag
     2461 taattagagc atgatataat gccaaagcca ttaaaaagaa aggatggtat gattatgaaa
     2521 aaaagattac tattattacc actattagca atgcttctag gtgggtgtaa cggcggaagt
     2581 agtgctactc caagcatcag caccccagaa agcattccta gtagtgaaga accaagtgtt
     2641 gaatcaagtg cagaaagcgt cagcgtttca attgatacga cgagctatga aatagcgaca
     2701 acgacaaatt attcaaaccg tgaattagat cattcaattg atatgactgc attagactca
     2761 cttggcacac aaaagatgtt agtcgttcct gtcgtgttta gtgacaccgc gcaccgcgca
     2821 actgaagaag tccatacgat gattaataaa gctttctttg gtgcaagtga agaaacaggc
     2881 tgggaatcag tcgcctctta ttatcacaaa acgagttttg gaaaattaac tttaactggt
     2941 gaagtactcc cttacttcta ttcacaatgg tcaacccatg cttttggcgc attagaagtt
     3001 tccccgacgc aagaatacgg tgaaggttat tactgggatc aaacgcacca cttaattgaa
     3061 gaaatttaca atacattaga tgccgaacta ttcttagaat atgaccaaga tggcgacggc
     3121 cacgttgatg ctttctggat ggtgtatatt gccgagatta aagcattagg cggggaagac
     3181 gatccgttct gggcttataa attttactgg aaccgttacg ctaataccga aaaaccaaca
     3241 ccaaatactt atgcgtgggc aagttataag tttgctgacg aaggtgtcgg ttataacttc
     3301 aatattcctg atgcgcatac ttatattcac gaaacaggac atatgtttgg cttacctgac
     3361 tattacgatt acgattcaaa aaccgcacca gcgggcgcgc ttgatatgat ggatcataac
     3421 gttgccgatc acaatatgta ttctaaatat cgcctcaact ggtcaaaacc atttgtcgtt
     3481 actggtaatg cggatattca tctccgtcca gcggaatcaa ccggtgactt tatccttatt
     3541 aaagacggat ggaacggaca tgtgtatgat gaatatattt taattgaata ctatacccca
     3601 accggactta atgaaaaaga tagtcaaggg agggggtatg aaacaaaatc aagtaacgta
     3661 tcgacaaaaa gctttgatat taatggggtg agaatgtggc acgttgattc acggctcatg
     3721 acttatgtct tcgatcctga atcattagaa ttaacaaatg cattttggac tgatgaaatt
     3781 gttgttggtg atccatttag ttacactatt attggtccaa gtaatacaac ttcacgtagc
     3841 agcacaccag aaggcgatag caaggttaat aagttattac acttaatgga cgctaccggt
     3901 acaacttcac gtgttggtaa ctggttacgg acaccaacca ctgcgggctc aacagcgtta
     3961 ttccaaactg gtgatgtcgt cgccgctgat gggtggaaga aatatatcca aacaagcgca
     4021 acatttaacg acggcacagc agtgggttac agtgtcgaga ttggtgaaat ggatgataac
     4081 ggtgtccaaa tcatcattcg taaagcttaa gctactgctt aacaaataag cgagttcatt
     4141 gaaaaacgca tacatttctc ttaaaatata aggatgaaat gtaaagttaa aagaaacaca
     4201 aagaaggtca ccttactcct aagtctcttg ctacttagta gttgtaattt ggaggtgacc
     4261 ttttttcctt acacacacca tataagtgac actgatactt atacaaggat cgaaacaagt
     4321 gattattata aagttggtgc ttttgcgcgc agttatcaag atgtattaaa aagtaattta
     4381 aatggtaatt cacgtgttgt accacttaag agtagtggcg aacaaaagat ccttgttttg
     4441 ccggtgaatt tccctgatta tgacttaaat gaattagatg gtaataatgg gaaaaccgcg
     4501 catattcata ttcaaaatgc tttctttggt gaaaatcaca caacacgctt ttattcagtt
     4561 gcggggtatt acgaagcttc tagttacggc aaacttaaac ttagtggtga tgtggcgccg
     4621 tggtacactt tacccgctga atatagcgtt agcgcgatta aaagcagagt acggagcaac
     4681 agcgataaac ttaatgaaac ggcagaaata cttaatttag cgctcaaaga atacgctaaa
     4741 agtaatgact taagtgcgtt tgatttagat gaaaatggtg tgcttgacag tgcttatgta
     4801 atatatagtt atccgtttga accaaaagat gtgaataata tcttctgggc ttttgccgcg
     4861 aacatgaata aaaaagatcc agaacttacc catcaagcga atg
//
LOCUS       DAOPCD010000045         4875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_14574_length_4875_cov_3.597303, whole genome shotgun sequence.
ACCESSION   DAOPCD010000045 DAOPCD010000000
VERSION     DAOPCD010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14574_length_4875_cov_3.597303"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..1121
                     /gene="prfA"
                     /locus_tag="PLQ55_01960"
     CDS             48..1121
                     /gene="prfA"
                     /locus_tag="PLQ55_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPK28731.1"
                     /translation="MEDVLRNRLETTKKRLSEIDEELLKPDITSDLDYFRDISVERSQ
                     IEEVVEKYNEYLKLEEDLESSLILMDDKDEEIAEFAKEQHKEIANRLPELINEIRVLL
                     IPKDPYDDKNIVVEIRGAVGGDEANIFAGDLFRMYVKYAERQGWQMQILDENSSAVGG
                     FSQISFMIKGKGVYSKLKYESGGHRVQRVPKTEASGRIHTSIATVLVMPEAEEVDVEI
                     RNEDLEIDTFRSQGAGGQNVNKVESAVRITHKPTGISVACQVEKSQIQNREIAMRLLR
                     ARVLAMKVAEQEEKIGAERKLKVGTGERSEKIRTYNYPQNRVTDHRIGFSTLKLDLVL
                     EGNLEEIIEALVHYDEQQKMVNS"
     gene            1121..1951
                     /gene="prmC"
                     /locus_tag="PLQ55_01965"
     CDS             1121..1951
                     /gene="prmC"
                     /locus_tag="PLQ55_01965"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPK28732.1"
                     /translation="MLTLYSLKQNTLKLHNFLDKNDVKLALMHVYNIDSYTDYVLKLH
                     TPIEEKQEFYAIINRLVKGEPIQYILGKAYFYDRYFFVNKNVLIPRVETEELVDIVLK
                     DVKENTGNLLDLGTGSGAIALTLKAHSKLSVFASDISKKALEVAQKNDCENSVTFVHG
                     DLLAPFINKEIAFDYIVANLPYIKREEEVEQKVKDYEPESALYNSDPNIFERLFKEVK
                     MLNFPKTGLSIYLEIGTNQDDEVSNLARRYLGENVKINVVNDMHGKKRFVKIKDIYGN
                     "
     gene            1941..2555
                     /locus_tag="PLQ55_01970"
     CDS             1941..2555
                     /locus_tag="PLQ55_01970"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPK28733.1"
                     /translation="METKIFKANEIHLLANIAKEGSIIAFPTETVFGLGVVYDNEEAF
                     NNLVKVKERKTDKPFTLMVGYTRDIENYAVLNTKTKNLIDVFMPGEITLIIEAKPNLP
                     KHVTLGSKYIGIRVSANYEVANLLNLIGKPLLVPSANKRDEKPALTVEEVYKVFNGDI
                     DGILEGETTSHIPSTIVKVDDKIELIREGSIPFNLIKEVWEEEL"
     gene            2552..2995
                     /gene="rpiB"
                     /locus_tag="PLQ55_01975"
     CDS             2552..2995
                     /gene="rpiB"
                     /locus_tag="PLQ55_01975"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPK28734.1"
                     /translation="MKVAIGSDHGGFIYKEAVKKHLEDKGITVIDVGSYHEDSVDYPL
                     YGIAVGEKVRDKAADLGIVICTSGEGITIAANKVKGVRAGIGYNDDVSRLIREHNNAN
                     VIGFGGSQMELKDVLRRVDIFLNARFESGGRHSRRVGIITEYENK"
     gene            3036..4205
                     /locus_tag="PLQ55_01980"
     CDS             3036..4205
                     /locus_tag="PLQ55_01980"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPK28735.1"
                     /translation="MQDFFICPTCGNNDLNKIGVRNGQKYCRACLPFQGQNAPKITNI
                     PLHIPITLSYELTTEQRRIAEEINANYINKQNSLVYAVCGAGKTELVYLAMQTALARG
                     HQIGFTVPRREVVIELAMRIKKTFPQARVISVFGDHHDALTGNIIVLTTHQLFRYEDY
                     FDLLIFDEIDAFPYVGDLVLESFFKRSVKGVYVLLSATPSTKLLKEFPKVGKVLTLFT
                     RFHNHKIPVPIVKRGFLLFNFMLLLKHLESFIKRKKPVLIFVPTIRLANLIYKLLRID
                     YKNGNVVHSKVKDNSAIIDDFKKGHYAYLVTTTVLERGVTIKGLQVIIYGADHAVYDT
                     ANLVQISGRVGRKKEESDGEVIFIVTKKNAAIKAAISDIEEKNAYLQSVLQRKAT"
     gene            4180..4770
                     /locus_tag="PLQ55_01985"
     CDS             4180..4770
                     /locus_tag="PLQ55_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPK28736.1"
                     /translation="MCFKEKQPNFNDLIRDTNVCNKCFRDLDVKFNVSKIAGVTYISV
                     FNYDEHFKTLLFHLKGLKDIVLAPLFLERFIPYFKVRFINYYLVPAPSTIESDKERGF
                     NHVIEIFKPLQRPFLRLITKKVAFKQSDLNYALRQKVKDKLVINNGNKVKGKNILIVD
                     DIKTTGATIMAMINLIKPYNPRKIAILTLAETSLSE"
BASE COUNT         1784 a          666 c          933 g         1492 t
ORIGIN      
        1 gaaaaagcat taaaaatata atataatacg accgcaagga taaatatatg gaagatgtat
       61 taagaaatag attagaaaca acaaaaaagc gtttatcaga gattgatgaa gagttactta
      121 aaccggatat tacaagtgac cttgattatt ttcgtgatat ttccgttgaa cgctcacaaa
      181 tagaagaagt tgtggaaaag tacaacgaat atttaaaatt agaagaagat ttagaatctt
      241 ccttaatctt aatggatgat aaagacgaag aaatagcaga atttgctaaa gaacaacata
      301 aggaaatcgc taacagatta ccagaattaa tcaatgagat tagggtgctt ttaattccaa
      361 aagatcctta tgatgacaaa aacattgttg ttgaaattag aggcgctgtt gggggagacg
      421 aggcaaatat ttttgcgggt gatctattta gaatgtatgt taaatatgct gaaagacaag
      481 gttggcaaat gcaaatcctt gatgaaaata gtagcgccgt tggtggcttt tcgcaaatct
      541 cctttatgat taaaggtaaa ggtgtttatt caaaacttaa atatgaaagt ggtgggcatc
      601 gtgtgcagcg tgtaccaaaa actgaagcaa gcggaagaat ccatacctca atcgctactg
      661 tgttagtgat gcctgaagca gaagaagttg atgttgaaat tcgtaatgaa gatttagaaa
      721 ttgatacttt tagaagtcaa ggcgctggtg gacaaaatgt taacaaagtt gaatccgctg
      781 tacgtattac gcataaacca actggtatca gtgttgcttg tcaagtggag aaaagccaaa
      841 ttcaaaatcg tgaaatagca atgcgtttat tacgcgcccg tgtcttagcg atgaaagttg
      901 ccgaacaaga agaaaaaatc ggtgctgaac gtaaacttaa agtcggcact ggcgaacgaa
      961 gtgaaaagat tagaacttat aactatccgc aaaatagagt tactgatcac cgtattggct
     1021 ttagcacatt aaaattagat ttagtgcttg aaggaaattt agaagaaatt attgaagcgc
     1081 tcgtgcatta tgatgaacaa caaaaaatgg taaatagtta atgttaactt tatactcttt
     1141 aaaacaaaat acattaaaac tacataattt tctcgacaaa aatgatgtaa aattagcatt
     1201 aatgcatgtt tataatattg attcgtatac agattatgtt ttaaaattgc atactcccat
     1261 tgaagaaaag caagagtttt atgcaattat taatcgtctt gttaaaggcg aacctattca
     1321 atatatttta ggtaaagctt atttttatga tcggtatttt ttcgtaaata aaaatgtgtt
     1381 aataccgcgt gttgaaactg aagaattagt ggatattgtg cttaaagatg taaaagaaaa
     1441 tacaggtaat ttacttgatt taggtacagg aagcggtgcg attgcattaa ctttaaaagc
     1501 tcattccaaa ttaagtgttt tcgctagtga tataagtaaa aaagcattag aagtcgccca
     1561 aaaaaacgat tgtgaaaata gtgtaacttt tgttcatggc gacttattag cgccatttat
     1621 taataaagaa atcgcttttg attatattgt tgcgaattta ccttatataa agcgcgaaga
     1681 ggaagttgaa caaaaagtaa aagattatga acccgaaagc gcactttata atagtgatcc
     1741 aaatattttt gaaagactat ttaaagaagt taaaatgtta aactttccca aaacaggatt
     1801 atcaatttat ttagagattg gcactaatca agacgacgaa gtaagtaatt tagcgcgccg
     1861 ctacttagga gaaaatgtta aaattaatgt tgtgaatgat atgcacggta aaaagcgctt
     1921 tgtcaaaatt aaggatattt atggaaacta aaatatttaa agcaaatgaa attcatttat
     1981 tagcaaacat tgcgaaagaa gggagtatta ttgcttttcc gactgagaca gtatttggtc
     2041 ttggtgttgt ttatgataat gaagaagcct ttaacaattt agtgaaagtt aaagaacgta
     2101 aaactgataa accgtttacc ttaatggttg gttatacacg agatattgaa aattatgcgg
     2161 ttttaaacac taaaactaaa aatttaattg atgtatttat gcctggagaa ataactttaa
     2221 ttattgaagc aaaacctaat ttaccgaaac acgtcaccct aggttctaaa tatattggca
     2281 taagagtaag cgctaattat gaagttgcta atcttttaaa tttaattggg aaaccactac
     2341 ttgtacctag cgctaataag cgggatgaaa aacctgcttt aacagttgaa gaagtatata
     2401 aagtctttaa tggtgatatt gacggcattc ttgaaggcga gacgacatcg catattccgt
     2461 ccacaatcgt taaggtggat gataaaatag aattaattag agaaggaagc attcccttta
     2521 atttaattaa agaagtttgg gaggaagaac tatgaaagta gcaattggca gtgatcatgg
     2581 tggctttatt tataaagaag cagtaaaaaa acatttagaa gataaaggga ttaccgtaat
     2641 tgatgtcggt tcatatcatg aagatagtgt agattatccg ctttatggga tagctgttgg
     2701 tgaaaaagtt cgtgataaag ctgctgattt aggaatagtt atttgtacat caggcgaagg
     2761 cattactatt gccgcaaata aggttaaagg ggtacgcgct ggtattggtt ataatgatga
     2821 tgtttcgcgt ctcattcgtg aacataataa tgccaatgta attggttttg gtggttcaca
     2881 aatggaatta aaggatgttt tacgccgtgt cgatattttt cttaatgcta gatttgaaag
     2941 tggtggtcgg cattcgcggc gtgtcggtat catcactgag tacgaaaata aataattact
     3001 gataaataaa gggtcttgct tacatactta taaatatgca agactttttt atttgcccga
     3061 cttgcgggaa taacgatctt aataaaatag gagtaagaaa cggccaaaaa tattgccgag
     3121 catgtttacc cttccaaggg caaaacgcgc ccaaaataac taatattcca ctgcatatac
     3181 caataacact aagttatgaa ttaacaacag aacaaaggcg aattgccgaa gaaatcaatg
     3241 caaattacat aaataaacaa aattcattag tgtacgctgt atgtggcgct ggtaaaactg
     3301 aattagtgta tttagcaatg caaactgcac tagcgcgtgg ccatcaaata ggttttacag
     3361 ttccgcggcg tgaagtcgta attgagttag cgatgcgcat taaaaaaact tttccgcaag
     3421 cgagagtaat cagtgttttt ggtgaccatc acgatgcttt aactggcaat attattgtac
     3481 ttactactca tcaacttttt agatatgaag attattttga tttattaatt tttgatgaaa
     3541 ttgatgcttt tccttatgtt ggcgacttag tgcttgaatc attttttaaa cgcagtgtca
     3601 agggtgtata tgttttacta agtgctactc cgagtacaaa gctattaaaa gagtttccaa
     3661 aagttggtaa agtgttaact ctttttacaa gatttcataa tcataaaatc ccagtgccaa
     3721 tagtgaaacg cgggttttta ctatttaatt ttatgttgtt attaaaacat ttagaatctt
     3781 tcattaaaag aaagaaacca gttttaattt ttgtaccaac gataagactc gctaatttaa
     3841 tatacaaact attacgcatc gattataaaa atggtaatgt agtgcattca aaagtaaaag
     3901 ataattcagc gataattgat gatttcaaaa aaggacatta cgcctactta gtaacgacaa
     3961 cagtcttaga aagaggggta acgattaaag ggttacaagt aataatttat ggcgcagatc
     4021 acgcagtgta cgacactgct aatttagtgc aaatttctgg aagagtcgga cgaaagaagg
     4081 aggagagtga tggtgaagtt attttcatcg ttacgaaaaa gaatgcggca atcaaagcag
     4141 caattagtga tattgaagaa aaaaacgcct atttgcaaag tgtgcttcaa agaaaagcaa
     4201 cctaacttta atgatttgat tcgtgacact aatgtttgta ataaatgttt tcgtgattta
     4261 gatgttaagt ttaatgtttc aaagattgca ggagttactt atattagtgt ttttaattat
     4321 gacgaacatt ttaaaacact acttttccat ctaaaaggtt taaaagacat tgtgctagcg
     4381 ccactttttc ttgaaaggtt tattccttac tttaaagtac ggtttattaa ttactattta
     4441 gtgccagcgc cttcaacaat agagagtgat aaagaacgcg gatttaatca tgtcatcgaa
     4501 atatttaaac cgctacaaag acctttttta aggcttatta caaagaaagt tgccttcaaa
     4561 caaagtgact taaactatgc actccggcaa aaagtaaaag ataaattagt aattaataac
     4621 ggaaataaag taaaaggtaa aaatatttta atcgttgatg atattaaaac aacaggtgcc
     4681 acaattatgg caatgattaa cttaataaaa ccctataatc cgcggaaaat tgcaatatta
     4741 acactagcgg aaacatcgct aagcgagtaa ctatcatatt tttatagaaa atatggtaaa
     4801 cttttataga tacatgatac gaaggagatt aactattctt aatcaagtta aatagtaaat
     4861 atatatcaaa aagat
//
LOCUS       DAOPCD010000046         4804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_14816_length_4804_cov_3.461992, whole genome shotgun sequence.
ACCESSION   DAOPCD010000046 DAOPCD010000000
VERSION     DAOPCD010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14816_length_4804_cov_3.461992"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2538)
                     /gene="polA"
                     /locus_tag="PLQ55_01990"
     CDS             complement(<1..2538)
                     /gene="polA"
                     /locus_tag="PLQ55_01990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPK28737.1"
                     /translation="MSKRLIIIDGNSLLFRAYYATAYMGTDKIMRTSTGVPTNAIFAF
                     ANMIVRIVNDLKADDHLFVAFDTGEKTFRHEEYDDYKANRQAAPDELVTQFPIAREFL
                     DALSIRHDEVVGYEADDLAGSLAKAAGKEGFKVELYTSDQDYLQLIDEDISVQLIRRG
                     LSDVRFLDPKGLYEEFGYHAYQVPDFKGLTGDASDNLKGVPGVGKVTATKLLAEYDTL
                     EKILENADNIKGKLGENIREHKATALQSKHLATILTDLPLPCPLNDFHYKGFDNSAVH
                     AFAQKYEMRQFLNNLKYTNSEVEEEIKAHEIKSFNEVKEKDYSYISFYVTASDEDYYK
                     ATPEFIYFSLGKNVYRISFNDLLLDPKLKALFSDEALLKYTYDYKRHAIMLSNHALDI
                     KGTVNDALVLTYVWDTNVAPNISAFLLTYGVNASSEEEETYAILVAKLSAIYQEGINK
                     LKKENLLQVYEDIEKPLTDVLIKMEKAGFNVDKDVLTAIGSEYKVILDNLTRTIFLLA
                     GHEFNINSPKQLATILFDELGLKANRKRSTSADVLNDLKDKHPIIPAILEYRKYQKII
                     STYIDGFIPHIHRDGKVHTTFNQALTTTGRLSSNNPNLQNISIRHEEGREIRKAFNAG
                     DKDVLAIDYSQIELRLLAAIANVPSLIDIFNTDRDIHSETAIALFPLEDLNYARRKAK
                     AVNFGIVYGISDFGLSEQLETTVKDARAIIQSFFKTYPEVWTYRQEVINELIKDGYVK
                     TLTGRKRYIPQINDPNYQTREFAKRAAMNAPLQGSAADLIKMAMIKVDEFLTKNKCKT
                     KLILQVHDELIFALDQDEHEIVDDLVNIMENVLDLPVKLKVEVKTGKT"
     gene            complement(2531..4732)
                     /locus_tag="PLQ55_01995"
     CDS             complement(2531..4732)
                     /locus_tag="PLQ55_01995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPK28738.1"
                     /translation="MTLNALKAKGLDNNKKYIERLTHELSIIRNLGYVNYFLIVSDYV
                     NFAKKAGILVGYGRGSAPGSLVSYLLGITHADPLEHNLLFERFLNPERVSLPDIDIDF
                     MDIRRGEVIDYIKKTYGEDKVANIVTFQTNATKASLRDTGNVFNIDPKFINRLSNSLG
                     NTNYNFRDAYRNIKAFRDLVDNDTYNLEIIKNATKIEGFPRQSGLHAAGIIIDGEPLA
                     RNLPTFFNNGLRVTQYEMGFLEEHGFLKVDILGLSNLTFVHNIINLVKENHNIDIDFY
                     NLKFDDPQIYDVIRNGHTLGLFQLESAGMRKAIATIKPTNFDDVSALLALYRPGPMKY
                     IDEYALRKEGKSPITYINDDLIDILKPTYGIMIYQEQIMQIAATMAGFSLGQADILRR
                     AIGRKDEKNILEQKDAFFAGAKKRGYSRKDTEKVFDDILRFADYGFNKSHSVVYAMLT
                     TALAYLKTYYPYEFYQEILRGLVSDNVKFSLARQELKALNIKISRPCVLKSTTEFIRT
                     NNSINFPLSAINGLNRESANIIIRLREQKAFSSVQDFFFRSFKAGVTAEEFIALIEAG
                     ALDSFNTNRAQLKVMFNTYLPTLDIGLFDNEAALHAIIVPKIKESDDRLDLEISRLRF
                     PLSGNPLDKLKLKDIKRVSHILELEDGYIKTYGVLTSLRLIKTKKGDEMAFATASDYE
                     EDINLVFFPRVLAEVKGMLTRNNIYIIAGKLETRDGQKQLVVNTLERVQNE"
BASE COUNT         1443 a          903 c          840 g         1618 t
ORIGIN      
        1 tgtttttccg gttttaactt ccacttttaa ttttactggt aaatcaagga cattttccat
       61 aatattcact aaatcgtcga caatttcatg ttcatcttga tcaagcgcaa agataagttc
      121 gtcatggact tgtaaaatta gttttgtttt acacttattc tttgttagaa attcgtcgac
      181 tttaatcatt gccattttaa ttaaatcggc agcgctgcct tgaagcggag cattcatcgc
      241 ggcacgcttt gcaaattcgc gtgtttgata gttaggatca ttaatttgcg ggatatagcg
      301 tttacgacca gttaatgttt taacgtaacc atccttaatt aactcattaa ttacttcttg
      361 tctataagtc catacctcgg gataagtctt gaagaaggac tgaatgatag cgcgggcgtc
      421 ttttactgtt gtttcaagtt gttcgcttaa gccaaaatca ctaatgccat acactatgcc
      481 aaagttaacg gcttttgctt tccgtcttgc gtaatttaag tcttcaagcg gaaatagtgc
      541 tattgcggtt tcactatgaa tatcgcggtc agtgttaaaa atatcaataa gactaggaac
      601 attagcgata gcggcaagta agcgtaactc aatttgacta tagtcaattg ctaaaacatc
      661 cttatctccg gcattaaatg cttttcttat ctcgcgtccc tcttcatggc gaatgctaat
      721 attttgtaag tttggattat tactgcttag tcgacccgtt gtcgttaagg cttggttaaa
      781 ggtagtatga actttcccgt cacgatgaat atggggaata aagccatcaa tataagttga
      841 aataatcttt tgatatttgc ggtattcaag aatagcagga ataattggat gcttatcttt
      901 taagtcattt aacacatcag cggaagtgga gcgtttacga ttagccttga gccccaattc
      961 atcaaaaagg attgtcgcta gttgttttgg ggaattaata ttaaattcat gacctgcgag
     1021 caagaaaatt gtacgcgtta agttatcaag aatgacttta tattcacttc caattgcggt
     1081 taacacatct ttatcaacat taaacccggc tttttccatc tttattaata catcagtcag
     1141 tggtttttca atgtcttcat aaacttggag caaattttct tttttaagtt tattaatgcc
     1201 ttcttgataa atagcactta atttcgcaac taaaatagcg taagtttcct cttcctcact
     1261 actagcgtta accccgtaag ttaataagaa agcgctgatg ttaggtgcca cattcgtgtc
     1321 ccatacataa gttaagacaa gtgcgtcatt aactgtgccc tttatatcta gagcgtggtt
     1381 acttagcata atggcatgac gtttatagtc ataagtatat ttaagaagtg cttcatcact
     1441 aaataaagct ttaagctttg gatcaagaag taaatcgtta aagcttattc gatatacatt
     1501 tttcccaagt gagaaataaa taaattctgg tgtcgccttg taataatctt catcactagc
     1561 ggtaacataa aaggaaatat aactataatc tttttcttta acttcattaa aacttttgat
     1621 ttcatgagct ttaatttctt cttcaacttc actatttgta tattttaagt tatttaaaaa
     1681 ttggcgcatt tcatactttt gcgcaaaggc gtgaacagca ctattgtcaa agcctttata
     1741 atggaagtca ttaagcggac acggtagcgg taagtcagtg agaatcgtcg ctaagtgttt
     1801 tgactgtaag gcagtagctt tatgttctcg aatgttttcc ccaagtttac ctttaatatt
     1861 gtcggcattt tcaagtattt tttctaaagt gtcatattcg gcaagtaatt ttgttgcggt
     1921 aactttacca acgcctggca ctccttttaa gttatcactt gcgtcccccg ttaacccttt
     1981 aaagtctggt acttgataag cgtgataacc aaattcttca tatagtcctt ttgggtctaa
     2041 aaagcgcacg tcacttagtc cgcggcgaat aagctgcacg ctgatatctt catcaattaa
     2101 ttgtaaataa tcttgatcac tagtgtataa ttcgacttta aaaccttctt tacctgctgc
     2161 tttagcaaga cttcctgcta agtcatcagc ttcgtatccc acaacttcgt cgtgacgaat
     2221 cgaaagcgca tcaagaaatt cacgagcaat cggaaattga gttactaatt catcgggtgc
     2281 tgcttggcga ttagccttat aatcatcata ttcttcatgt ctaaatgttt tttcgccggt
     2341 atcgaaggca acaaataaat gatcgtcagc ctttaaatca ttaacaatgc gcacaatcat
     2401 attcgcaaaa gcaaaaatgg cgtttgtcgg tacgccagtg cttgtgcgca taattttatc
     2461 tgtgcccata tatgcagtag cgtaatatgc tctaaaaagc aaagaatttc catctataat
     2521 aataagtcgt ttactcattt tggacccttt ctaaagtgtt aactacaagt tgcttttggc
     2581 cgtccctggt ttcaagttta ccagcgataa tataaatatt attgcgggtt aacatccctt
     2641 taacttccgc aagtacacgc ggaaagaaga caagattaat gtcttcttca taatcactag
     2701 cggtcgcaaa agccatctcg tcaccctttt tcgtcttaat aagccgtaat gatgttagta
     2761 caccataagt tttaatgtaa ccgtcttcta attctaaaat atgactaacg cgtttaatgt
     2821 cttttagttt aagtttatca agcggattac ctgaaagcgg gaagcgcagc ctacttattt
     2881 ctaaatcaag acggtcgtca ctctctttta tcttaggaac aataatcgca tgtaaagccg
     2941 cttcattatc aaataagcca atgtctaaag ttggtaagta ggtgttaaac attaccttta
     3001 actgtgcgcg attagtgtta aaagagtcaa gtgccccagc ttcaataagg gcgataaatt
     3061 cttcagcggt aacaccagcc ttaaaactgc ggaagaagaa gtcttgcaca ctactaaaag
     3121 ctttttgttc acgtaatcta ataatgatat tcgcggattc cctgtttaat ccgtttatcg
     3181 cactaagtgg gaaattaatc gagttattag tgcgaataaa ttcagttgta gattttaaga
     3241 cacaaggcct acttatcttg atgttaagtg cttttaattc ttgtctcgct aaggagaatt
     3301 taacattatc acttactaaa ccgcgtaata tttcttgata gaattcataa ggataataag
     3361 ttttaagata cgctaaagca gtagtgagca tcgcgtacac tacagagtgc gatttattaa
     3421 atccgtaatc agcaaagcgt aaaatatcat caaacacttt ttcggtatct tttcttgagt
     3481 agcctcgctt tttagcacca gcgaaaaatg catctttttg ctctaggata tttttttcat
     3541 ctttacgccc aatcgcccgt cgtaagatat cggcttgccc aaggctaaag ccagccattg
     3601 tcgcggcaat ttgcataatt tgttcttgat aaatcataat gccgtatgtt ggttttagga
     3661 tatcaattaa gtcgtcattg atgtaagtta tcggtgactt tccttcctta cgcagagcgt
     3721 attcatcaat atatttcatc ggaccaggac gataaagcgc aagtaaggcg ctaacatcat
     3781 caaaattagt cggtttaatt gtcgcaattg ctttccgcat tccggcgctt tcaagttgga
     3841 aaagtcctaa tgtatgacca ttacggatta catcataaat ctgcggatca tcaaatttca
     3901 aattataaaa atcaatgtcg atattatgat tttctttcac taaattaatg atgttatgaa
     3961 caaaggtaag gtttgatagt cctaaaatat caacttttaa aaatccgtgt tcttcaagga
     4021 atcccatttc atattgtgta acgcgtaacc cgttattaaa aaatgttggt aagtttctcg
     4081 cgagcggttc gccgtcaatt ataataccag cagcgtgaag cccactttgc cgcggaaatc
     4141 cttcaatttt agtagcattt ttaataattt ctaagttata agtatcgttg tccactaaat
     4201 cacggaatgc tttaatgtta cgataagcat cacggaagtt atagttagtg ttacctaaag
     4261 agttacttaa gcgattaata aatttgggat caatgttaaa aacattacct gtatcacgca
     4321 acgatgcttt tgtggcatta gtttggaaag tcacaatatt agcgacttta tcttcaccat
     4381 aagtcttttt aatataatca attacttcac cgcgtctaat atccataaag tcgatatcaa
     4441 tgtctggcaa agagacacgt tcaggattta aaaagcgttc aaaaagtaaa ttgtgttcaa
     4501 gtggatcagc gtgggtgata cctaaaagat aactaacaag tgaacccggt gcactacccc
     4561 taccgtaacc gactaagatt ccagcttttt ttgcaaaatt aacataatca ctaacgatta
     4621 agaagtagtt aacatagcct aaattacgaa tgatacttaa ttcatgtgtg agtcgttcta
     4681 tgtatttttt attattatct aagccttttg ctttaagcgc atttagtgtc aattcatcaa
     4741 ttttcttatt ggatcatatg cgccaggaag tgggaaattt aagtcgtgtt taacgcttaa
     4801 atca
//
LOCUS       DAOPCD010000047         4777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_14892_length_4777_cov_3.074756, whole genome shotgun sequence.
ACCESSION   DAOPCD010000047 DAOPCD010000000
VERSION     DAOPCD010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14892_length_4777_cov_3.074756"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLQ55_02000"
     CDS             complement(<1..208)
                     /locus_tag="PLQ55_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28739.1"
                     /translation="MRLFQTAALIFGIGAAASFFSGYFLTNGDFIREIILSVSIWFFA
                     IFFLFVPHITKKILIQNILFFITSV"
     gene            complement(221..979)
                     /locus_tag="PLQ55_02005"
     CDS             complement(221..979)
                     /locus_tag="PLQ55_02005"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPK28740.1"
                     /translation="MDYNALLPIIFYLCACFYSFFGIYTLATNAKSRTNWQFFFLMIS
                     LTIWSFTYAMAYSVDSAETRIMWKSLGVFGWSLFYAFFLRFAIILTKRNEPSKKPFLQ
                     VLFYLPALITIILFGPFGFLMGMQYEFVPGETGLFQAIINNIGQIWFGLYPSAYTIIS
                     LVILIRWRRTIDRESPLRRLLSIFLISIILPFIIGIFLGVFPRFFGLENLPRLTVAFL
                     IPPAVLLFIALRKFGMVFETKTRRDFSPLDPKTT"
     gene            1278..1490
                     /locus_tag="PLQ55_02010"
     CDS             1278..1490
                     /locus_tag="PLQ55_02010"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPK28741.1"
                     /translation="MILPIANTERDYVILEVAGTDKLRQYLASLGIIKGKSLRIMSQG
                     KTGTIILVNETRLALNYDIAKLITIA"
     gene            1505..1792
                     /locus_tag="PLQ55_02015"
     CDS             1505..1792
                     /locus_tag="PLQ55_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPK28742.1"
                     /translation="MNEEKIIIKVGDLAKLSIVPIGTSGVIHLVSGQGRLRRRLLDMG
                     LTPGAEVFLRKQAPLGDPLELTLRGYELSIRKSEADLIVIRVTDLDAKGDK"
     gene            1794..4067
                     /locus_tag="PLQ55_02020"
     CDS             1794..4067
                     /locus_tag="PLQ55_02020"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HPK28743.1"
                     /translation="MKKNIRVALLGNPNSGKTTLFNSLTGSTAYVGNWPGVTVEKKSG
                     TYYFKGKGKDREGIDIIDLPGIYSLSPYTPEEVISRNYILKENPDVVINVIDATNLER
                     NLYMTTQLLEIDVPVVLAVNMTDALAESGKTIDYAKLKEKLKIPVVPISALEEKGLDN
                     LMKVTKVEANKKREAYSVITDKKIEKVTEIYKKYKIANPLFHAIKALEKDEIEESENK
                     EAYADVQSLMAQDKEDYEAVIADLRYQFLTPLCAEISTKPHKSEKEKWTKSDRIDQVL
                     THKIFGMLIMIVALFFVFHFTFSGDLLFLNAFGVNFGEGYEGFLSIMVGGEEYRPFAG
                     LIYTDGGINSIGEFFHVLAGDNETGILGLISLGIKALLLKWNAPEWVVGFFYDGLLNG
                     VTAVLGFVPQIMILYTFFSIMEDSGYMARIAFMLDRIFRKFGVSGRAFIPMIMGFGCA
                     IPAMINTRTLATEKERIKTIRVIPFFTCGAKAEFIAIIAAAIAAAVGVDAGLFTFSMY
                     MLGILVAIVSVIVMNKTTQREKVPPFIMELPTYRTPQLKALTRHVYNKGKHFVIKAFT
                     IILASTIVIWLLANFGWDWQMVDENGTGSILQSLGSFIQPLFTPLGFGVQAGEHGWTL
                     TVASIQGIVAKENVTATAETLASIIDVDGFTGIVEAIGLSNAAVVAFSVFNLLTIPCF
                     AAIATAKGELSNRKVYRWTIAYWFLLSYGLGALTYVSFAYVWTLAITIPVIALLIALL
                     IIYDRHKKKQELLLENE"
     gene            complement(4350..4715)
                     /locus_tag="PLQ55_02025"
     CDS             complement(4350..4715)
                     /locus_tag="PLQ55_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPK28744.1"
                     /translation="MGLKPSGEMYLKTIYILTKTVHPVRSIDIAEYMNFSKPSVSRAV
                     GNLRKEKYISVNEDGHIFLTPKGLDAAQNIYDRHKVLTKALRILGVSEETALNDACEI
                     EHIISDESFEALKQYLKNK"
BASE COUNT         1613 a          754 c          907 g         1503 t
ORIGIN      
        1 ctacactagt gatgaaaaag agaatatttt gaataagaat cttcttcgta atatgtggca
       61 caaatagaaa gaatatagca aagaaccaaa tagaaacaga tagaataatt tccctaatga
      121 aatcgccatt tgttaagaaa taaccggaga aaaatgatgc agcggcacca atcccgaaaa
      181 ttagcgctgc tgtttggaat aagcgcaagc ggctttcttt ttatgttgtc ttgggatcaa
      241 ggggtgagaa gtcacgcctt gtttttgttt cgaataccat tccaaattta cgcagtgcga
      301 taaataataa aaccgcaggc ggaatcagaa atgcaacagt gagccgtggt aagttttcta
      361 aaccgaaaaa gcgcgggaat acaccaagga atatgccgat aataaagggt aatatgattg
      421 aaattaagaa gattgataaa agccgtctaa gcggagattc acgatcaatt gtcctgcgcc
      481 atctaataag tatgactaga gaaataattg tatatgctga agggtataat ccaaaccaaa
      541 tttgaccaat gttattaata attgcttgaa ataatcctgt ttcacctggc acgaattcat
      601 attgcatacc cattaaaaag ccaaaaggac caaataagat tattgttatt agtgcaggta
      661 aataaaataa aacttgtaag aagggctttt tacttggttc atttcttttc gttagaatta
      721 tggcgaagcg gaggaaaaaa gcataaaaaa ggctccaccc aaaaacacca agtgacttcc
      781 acattattct tgtttcagca ctatcaacag agtaggccat tgcatatgta aatgaccaaa
      841 ttgttaacga aatcattagg aagaaaaact gccaattcgt gcgacttttc gcattagtgg
      901 ccaaagtata tatgccaaaa aaactataga aacaggcgca aagataaaag attatcggaa
      961 gcaatgcatt ataatccata gttttttgct gttcctccta ctattaatca attatttgat
     1021 taacacaagc aaattctaac acatagttag aaaaaaagca caacaatatt aaaaaaatgc
     1081 agaaattatg aatatatttt acaattggtg ctctttttaa ctgtttataa aacgactcta
     1141 tcacattaat aaactctata acaataaatg tttcgatgta aatttatgaa tattttttaa
     1201 aatttacaat attgcttgaa ataccggtta gcaacggcta acatttcctt gttatcaatg
     1261 actaaatagg ggattttatg atattaccaa tagcaaatac ggaacgcgat tatgtcattt
     1321 tagaagtagc gggcacggat aaactccgac aatatttagc gtccctagga attattaaag
     1381 gtaaatcact taggattatg tcacaaggta aaacaggcac gattatttta gtaaatgaaa
     1441 cacgtcttgc ccttaattat gacattgcca agctaataac tatcgcataa ggaatggtag
     1501 caatatgaat gaagaaaaaa taattattaa agttggagat ttagcaaaac taagtattgt
     1561 tccaattggt acaagcggag taatccactt agtaagtggc caaggacggt tacggcgtcg
     1621 gcttctagat atgggtttaa caccaggcgc tgaagtgttt ttgcgtaaac aagcgccact
     1681 tggtgatcct ttagaactta cattacgcgg ttatgaatta tctattcgta aaagtgaagc
     1741 cgatttaatt gtaattagag ttacagattt agatgcgaaa ggtgataaat aagatgaaga
     1801 aaaatattag agttgccttg ttaggtaatc caaattctgg aaaaacaaca ctttttaata
     1861 gtttgactgg ttcaacggcg tatgttggta attggccagg tgttactgtt gaaaagaaga
     1921 gcggaactta ttattttaaa ggcaaaggta aagatagaga aggcattgac attattgact
     1981 taccaggtat ttattcttta tctccttaca cccctgaaga agttatttca cgaaattata
     2041 tattaaaaga aaatcctgat gttgttatta atgtaattga cgcaactaac ttagagcgta
     2101 atttatatat gacaacacaa cttttagaaa tcgatgttcc tgttgttctt gctgtgaata
     2161 tgactgatgc tctagcggaa agtggcaaaa caattgacta tgcaaaactt aaagaaaaat
     2221 taaaaatccc tgtagtgccg atttctgcat tagaagaaaa agggctagat aatttaatga
     2281 aagtaacaaa agttgaagcg aataagaaac gtgaagctta tagtgttatt actgataaaa
     2341 aaattgaaaa agttaccgaa atttataaaa aatataaaat cgctaatccg ttatttcacg
     2401 cgattaaggc actagaaaaa gatgagattg aagaatctga aaataaagaa gcttacgctg
     2461 atgttcaatc attaatggca caagataaag aagactatga agctgtaatt gctgacttac
     2521 gttatcagtt tttaacccca ttatgcgcgg aaataagtac aaaaccgcat aaaagtgaaa
     2581 aagaaaaatg gacaaaatct gaccggattg accaagtgtt aacgcataaa atattcggaa
     2641 tgctaatcat gattgtcgcc ttattttttg tatttcattt tactttcagt ggagacctac
     2701 tatttttaaa tgcttttggt gttaattttg gtgaaggtta tgaaggtttc ttaagtatta
     2761 tggtaggtgg cgaagaatat cgaccattcg ccggccttat ttacactgat ggtgggatta
     2821 attctattgg tgaatttttc catgtactag caggtgataa tgaaactgga atactgggat
     2881 tgatatcttt aggaattaaa gcactattac ttaaatggaa tgctcctgaa tgggtcgttg
     2941 gttttttcta tgacgggcta ttaaacggtg ttactgctgt tttagggttt gtcccacaaa
     3001 ttatgattct ttacactttc ttttcgatta tggaagatag tggatatatg gctcgtattg
     3061 cctttatgtt agatcgtatt ttccgtaaat ttggtgtttc tggtcgtgct tttattccaa
     3121 tgattatggg ctttggctgt gcaattcctg caatgattaa tacacgcaca ctagcgactg
     3181 aaaaagaaag aattaaaaca attcgcgtta ttccgttttt cacttgtggc gcaaaagctg
     3241 aatttattgc cattattgcc gcagcgatag cagcagcagt tggtgttgat gctgggctct
     3301 ttactttttc catgtatatg cttggtattt tagtagcgat tgtttcagtt atagtaatga
     3361 ataaaacaac gcaaagagaa aaagtaccac cctttattat ggaattacca acatatcgca
     3421 cacctcaatt aaaagcgctc acgcgtcatg tttataacaa aggtaaacac tttgtcatca
     3481 aagcatttac tatcatttta gcgtcgacga tcgtgatttg gcttttagca aactttggtt
     3541 gggattggca aatggtcgat gaaaatggta caggctcaat tttacaaagt cttggttcat
     3601 ttattcagcc cttatttaca ccgcttggtt ttggtgtaca agctggtgaa cacggctgga
     3661 cattaacggt cgcttcaatc caaggtatag tggccaaaga aaatgttacc gcaacagcgg
     3721 aaacactagc gagtattatt gatgtagatg ggtttaccgg tattgtcgaa gcgattggac
     3781 taagtaacgc tgctgttgtg gcctttagcg tgttcaactt actaactatt ccttgttttg
     3841 cggctattgc tacagctaaa ggtgaattaa gtaatcgcaa agtatatcgg tggacaattg
     3901 cttattggtt ccttttaagt tatgggttag gcgcattaac ttatgttagt ttcgcttatg
     3961 tgtggacact ggcgataacg ataccagtta tagcactatt aattgccttg cttattattt
     4021 atgatcgcca taaaaagaaa caagaattac tattagaaaa tgaataacaa aggaagatta
     4081 aaattttgca ttggacagaa atattcacga ttatatttat tattgttgta ctaactagca
     4141 ctattcttta ttttgttaaa aaaagacaaa aaggcgaaac aggatgtggg gttagttgtg
     4201 gtgggtgtcc cacgacaaat attgacatta agtcatatta tcgtaaacat tataagtcgc
     4261 ttaaataaaa caagaaatga ttattaaaaa cgggctgtta atgaagatta tattcataag
     4321 cagcccttta tttttatgaa gtttaagtat tatttatttt ttaggtactg tttaagtgct
     4381 tcaaaggatt catcgctaat aatatgttcg atttcacaag cgtcattaag tgccgtctct
     4441 tcacttactc ctaaaatacg cagcgcctta gttagcactt tatgtctgtc atatatattt
     4501 tgtgcagcat caagcccttt aggcgtaagg aaaatgtgac cgtcttcatt aacgcttata
     4561 tacttttctt tccgtaaatt accgacagcg cgactcacac ttggtttaga aaaattcatg
     4621 tattcagcaa tatcaattga ccgaacagga tggactgtct tagttaaaat ataaattgtt
     4681 tttaaataca tctcaccaga tggttttaga cccatataac cgacttgtat tatctaatca
     4741 tgccccggta cttcgattag gtaatacatc tcctttc
//
LOCUS       DAOPCD010000048         4767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_14923_length_4767_cov_3.740662, whole genome shotgun sequence.
ACCESSION   DAOPCD010000048 DAOPCD010000000
VERSION     DAOPCD010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14923_length_4767_cov_3.740662"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="PLQ55_02030"
     CDS             complement(<1..1053)
                     /locus_tag="PLQ55_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase-associated domain-containing
                     protein"
                     /protein_id="HPK28745.1"
                     /translation="MKKLFTILLASLMVFGSTDKSAPKRAVGPKPTLEVEKVMTPEQA
                     YAEPHIVFHYFRNDANYARYAMWIWGQDEDGSEYNFTETDAYGKYLHVPVANFTDQTQ
                     INFLLKTKGSWEWQTPDVNIQYSDFTFDTVTNAYHFYLVNGLITVFASAEEAKANQVT
                     ESIFASKNTVRVSTNNEPTTFVIKADGTNVTSGAAEHTYNNNTWSYVFTVNLGAAFLP
                     DFSKAYTVEVAFTAGSAVISEIGLSGLFDDPYFIENMTYNGQDLGVTYSPTKSTFKAW
                     APTASELKLRIYESGTPVALNADLGNDTYTEHDFVRTEKGVWSAELAGDYHGKYYTFV
                     AKHPTGYVELIDPYAKS"
     gene            complement(1070..2533)
                     /gene="malQ"
                     /locus_tag="PLQ55_02035"
     CDS             complement(1070..2533)
                     /gene="malQ"
                     /locus_tag="PLQ55_02035"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPK28746.1"
                     /translation="MRQAGILLAVSSLPGKHGIGDFGPSGRTFIRKASKTGFKLWQIL
                     PVNPLGYGNSPYQPFGSKPFDEIYISLELLTKEGLLPEVPSYFARSKKVRYDKVRKFK
                     LRHLKRAFNNFKKQGRRGFKKWVKDNKWVKDYAIFITFKRDNNLVQWHYWKEAQREWS
                     KTRRGVNLKPFAKAIEFEMWLQYIAYKQWMDLRAYAHRYGMQIVGDIPIYVGIDSVDV
                     WTNRKAFLLDENDEPTFVGGVPGDYFNADGQLWGNPLYDWDYLAETGFEFWVDRLRYN
                     ARLFDIIRIDHFRGFDSYWKIPAGSSRANDGHWAYPPGYALFDKIYEELPDIKIIAED
                     LGDLRPEVLELRDHYNLPGMEITSFTFNPFEKRDENAWLENRCIYPGTHDNSPIRGYF
                     KWKDKKFMKAANKLLDDLGFNEFDNFYDNFMAMNFGSDARYAIFQMQDLLRLGDEAKM
                     NSPSTIGSPNWEWKMQDFKTFDEESSLYAYLIEKYNR"
     gene            complement(2535..4142)
                     /locus_tag="PLQ55_02040"
     CDS             complement(2535..4142)
                     /locus_tag="PLQ55_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPK28747.1"
                     /translation="MKAKQSLRALMLIFPLLFMASCKDLPSESVSSEEILQAPTDYEI
                     DESITNEKGGVYYQIFVRSFADTNADGHGDLKGITDKIPYLQELGVKGIWLTPINPSP
                     SYHGYDVTDYKAIHPDFGTLDDFDTLISVAAEHNIDVIIDLVINHTARTHPWFLEGRR
                     RFNTPEYNPNDPNDKGNWYDFYIENGEVKVKIGYFGDHMPELLLNNTAVREEITSISK
                     FWLDRGVKGFRLDAAGHFFETHPENIDFLGWFSDMVKSYKPDSYIVAEAWENFNVQKY
                     YYAGVESLFNFDGANATGFIIDSIMARSGPQLSHYISNAFSDAYAINENALMAMFLTN
                     HDMDRSSQMFTGDQLERQKLGASVYILTPGVPFMYYGEEIGLKGSRQGAMTDANRRLP
                     MIWKRVGDKHRTNPPIGTTYNMANQVKYGAEDLLSEPFSLLNHYRKVINVRNFHPWIK
                     NARAYNLRLNNNGVTGLTLKEHNGQRELHVIHNLLAEEVSLNLTRFISEYEVEIIHDI
                     YTTGTRAKYVDGSLTLAPYSSVVLRRK"
     gene            complement(4145..>4767)
                     /locus_tag="PLQ55_02045"
     CDS             complement(4145..>4767)
                     /locus_tag="PLQ55_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPK28748.1"
                     /translation="TIVLLTSYTFSRLRFKARKGLMKFLMILGMFPGFMSMIAIFHIL
                     KAIGLLPTETRPEVPVSNYLISLILVGTSGAAMGYLVSKGYFDTISKSIDEAAEIDGA
                     NKAQIFWHIILPLSKPILIYTALGAFMGPWGDYIFVATITRGKREAWNIARGLYDMIN
                     GGLEKLGKYFTLFCAGAVLISIPITILFVFMQRYYVSGVTGGAVKG"
BASE COUNT         1394 a          991 c          894 g         1488 t
ORIGIN      
        1 acttttagcg tatggatcaa ttaattcaac atagcctgtt gggtgtttag cgacaaaagt
       61 gtagtatttg ccgtgataat cacccgcaag ttcagcgctc catacacctt tttcagtgcg
      121 cacgaaatcg tgttctgtat atgtgtcatt accaagatca gcatttagag cgaccggtgt
      181 accactttca taaatccgta attttaattc agaagcagtt ggtgcccatg ctttaaatgt
      241 tgatttagtg ggtgaataag taacccctaa gtcttggcca ttataagtca tgttttcaat
      301 aaaataagga tcatcaaata atcccgataa accaatttca ctaatgacag cactgcctgc
      361 ggtaaaagcg acttcgacag tgtaagcttt actaaagtca ggaaggaatg ctgcgcctaa
      421 attaacggta aacacatatg accatgtgtt gttattataa gtatgctctg ccgctccact
      481 tgtgacattt gtgccatcag ccttaatcac aaaagttgtt ggctcattat tagtggaaac
      541 acgaactgta tttttacttg caaagatact ttcagttact tggtttgctt ttgcttcttc
      601 agcactagca aatacagtaa ttaaaccgtt tactaaataa aagtgatagg cgttagtaac
      661 ggtatcaaat gtaaaatcac tatactgaat atttacatca ggtgtttgcc attcccagct
      721 tccctttgtt tttaagagga agttaatttg tgtttgatcg gtaaaattcg ccactggcac
      781 atgtaaatat tttccgtatg catcagtttc ggtgaagtta tattctgatc catcttcatc
      841 ttgaccccaa atccacatcg cataacgcgc ataattagcg tcatttctaa aatagtgaaa
      901 gacgatatgc ggttcagcgt aagcctgctc tggcgtcatt actttttcca cttctaaagt
      961 aggttttgga cctactgctc ttttcggtgc acttttatca gtactgccaa acaccatcaa
     1021 agaggcgaga agtattgtga aaagtttttt cataaatttc tctcctttct taacgattat
     1081 atttctcaat taaatatgcg tataaagaac tttcttcatc aaaagtctta aagtcttgca
     1141 ttttccattc ccagtttggg ctaccaattg tacttggaga attcatcttg gcttcatccc
     1201 ctaaacgcaa taaatcctgc atttggaaaa tagcgtaacg cgcgtcacta ccaaaattca
     1261 tcgccataaa gttgtcgtag aaattatcaa attcattaaa gcctaaatca tctaataatt
     1321 tattcgctgc tttcataaac tttttatctt tccacttgaa atagccgcga attggcgagt
     1381 tatcatgtgt acctggataa atacaacgat tttctaacca tgcattttca tcgcgctttt
     1441 caaagggatt aaaagtgaaa gatgtaattt ccattcctgg taaattataa tggtcacgga
     1501 gttctaatac ttctgggcgt aagtcaccta agtcttcagc gataattttg atatctggta
     1561 attcttcgta tattttatca aagagtgcgt agcctggcgg ataagcccag tgtccatcat
     1621 tagcgcgact gctaccggcg ggaattttcc aataggaatc aaaaccgcgg aagtggtcaa
     1681 tgcggataat atcaaaaagt ctagcgttat aacgcagtct atcaacccaa aattcaaagc
     1741 cggtttccgc taagtagtcc cagtcataaa gcgggttacc ccacagttga ccatcagcgt
     1801 taaagtagtc gcctggcaca ccaccaacga atgttggctc atcattttcg tcaagcaaga
     1861 atgctttacg attagtccaa acgtcaacac tatcaatacc cacgtaaatc gggatatcgc
     1921 caactatttg catcccgtaa cgatgagcgt aagcgcggag atccatccat tgtttataag
     1981 caatgtattg taaccacatt tcaaattcaa tggctttcgc aaatggtttt aagttcacac
     2041 cacggcgcgt tttactccat tcacgttgcg cttctttcca gtaatgccat tgcactaagt
     2101 tgttatcacg tttaaaggta ataaagatcg cgtaatcttt aacccattta ttatctttaa
     2161 cccatttttt aaacccgcgt cggccttgtt ttttaaaatt attaaaggcg cgttttaaat
     2221 gacgcagttt aaatttacgc actttgtcat agcgaacttt tttactgcgc gcaaagtagc
     2281 taggaacttc cggtagtaat ccttcttttg ttaaaagttc gagactaata taaatttcat
     2341 caaatggttt cgagccaaat ggttgataag gcgaattacc gtaaccaagc gggttaacag
     2401 gtaagatttg ccataattta aagccagttt tgctagcttt acgaataaaa gtgcggccac
     2461 ttggaccaaa gtccccaata ccgtgtttgc ccggtaaact actgaccgct agtaaaatgc
     2521 ctgcttgacg cattttattt cctccttaat acgacactgc tataaggggc tagtgttaat
     2581 gagccatcaa catactttgc ccttgtgccg gtagtgtaaa tgtcatgaat aatttctact
     2641 tcatattcac taatgaagcg tgtaaggttc agcgaaactt cttcggcaag taagttgtga
     2701 attacatgca gttcacgctg gccattatgt tcttttagtg ttaaaccagt aacaccgtta
     2761 ttattaagtc ttaaattata agcgcgagcg tttttaatcc atgggtgaaa gtttcttaca
     2821 ttaattacct tgcggtaatg gttaagtaaa ctaaacggtt cactaagtaa atcttctgcc
     2881 ccgtacttta cttgatttgc catattgtaa gtagtaccaa ttggtggatt agtgcgatgt
     2941 ttatcaccga ctcttttcca aatcattggt aagcggcgat ttgcatcagt catcgcgcct
     3001 tgtctactac cttttaaccc aatttcttcc ccataataca taaagggaac accaggcgtt
     3061 aagatgtaga cgcttgcgcc gagtttttgt cgttctaatt gatcacctgt gaacatttga
     3121 ctagaacggt ccatatcatg gttagttaag aacattgcca ttagtgcatt ttcattaatt
     3181 gcatatgcgt cactaaaggc gttgctaatg taatgcgaaa gttgcggacc agagcgcgcc
     3241 ataatgctgt caattataaa cccagtcgcg ttagcgccat caaaattaaa gagtgattca
     3301 accccggcgt agtaatactt ttgcacatta aagttttccc atgcttcagc gacgatataa
     3361 ctatcgggtt tataactttt aaccatatcg ctaaaccacc ctaaaaagtc gatgttttcc
     3421 ggatgagttt caaagaaatg acccgcagcg tcaagtctaa accctttaac cccgcgatca
     3481 agccaaaatt tactaatgct cgtaatttct tcacgtacgg ctgtattgtt gagtagtagt
     3541 tcaggcatat ggtcgccaaa gtaaccaatt ttaactttta cttcgccatt ttcaatgtaa
     3601 aagtcatacc agttaccttt atcatttggg tcgttaggat tgtattctgg tgtattaaac
     3661 cgtcttctcc cctcaagaaa ccacgggtgc gtgcgcgcag tgtgattaat aacgaggtca
     3721 ataataacat caatattatg ttctgctgcg acactgatta aagtatcaaa gtcatcaagc
     3781 gtaccaaagt caggatgaat cgctttataa tcggttacat catagccatg ataactaggt
     3841 gaagggttaa tcggggttaa ccaaatcccc ttcactccta gttcttgtaa atatggaatt
     3901 ttatcagtga taccttttaa gtcgccatga ccatccgcat ttgtgtcagc gaatgagcgc
     3961 acgaaaattt gatagtatac gccgcctttt tcattagtga ttgactcatc aatttcgtaa
     4021 tccgtcggcg cttgtagtat ttcttcactc gaaacacttt cgcttggtaa atctttacaa
     4081 cttgccataa atagtaaggg gaagataagc attaaagcac gaaggctttg ttttgctttc
     4141 atggttatcc cttaactgct ccacctgtta caccgctgac ataatagcgt tgcatgaaga
     4201 cgaagagaat tgtaattgga attgaaatga gtactgctcc tgcacagaag agcgtgaagt
     4261 acttaccaag tttttctaga ccgccattaa tcatgtcata aagtccgcgg gcaatgttcc
     4321 acgcttcacg tttaccacgt gtaatcgtgg caacgaagat atagtcaccc cacgggccca
     4381 taaatgcacc taaggcggtg taaattagaa ttggttttga aagtggcaaa ataatatgcc
     4441 agaagatttg tgctttatta gcaccgtcaa tttccgctgc ttcatcgatt gatttactaa
     4501 tcgtatcaaa gtagccttta gagacgagat accccatcgc tgctccgctt gtaccaacaa
     4561 ggataagtga gatgaggtaa ttagaaacgg gaacttctgg tcgtgtttcg gttggtaata
     4621 atccaatcgc ttttaagatg tggaaaattg caatcattga catgaagcca gggaacattc
     4681 cgagaatcat taaaaatttc ataagacctt tgcgcgcttt aaaacgaagc cggctaaagg
     4741 tataacttgt aagtaagaca atagttg
//
LOCUS       DAOPCD010000049         4730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_15067_length_4730_cov_3.242781, whole genome shotgun sequence.
ACCESSION   DAOPCD010000049 DAOPCD010000000
VERSION     DAOPCD010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15067_length_4730_cov_3.242781"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PLQ55_02050"
     CDS             complement(<1..356)
                     /locus_tag="PLQ55_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28749.1"
                     /translation="MEEQEKRVDEVNQSEVEVMLEPLNYGSNHHEESRIKSPGFGYGL
                     FFSSLILLFLASFTIDFIVDALVGEGNEVTAFAYKNFGTYVVTFSLITVFLFLTKTLL
                     PLVKKFTKIRPYIHGL"
     gene            433..1035
                     /locus_tag="PLQ55_02055"
     CDS             433..1035
                     /locus_tag="PLQ55_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPK28750.1"
                     /translation="MGYVINKSYINEIVVEKSRFIALLCPLTNENDFDEYFKESKTTY
                     KDARHYTYAFRYGNLERASDDGEPQNTAGLPLLNLLKNHDLVNTALIVIRYFGGKKLG
                     AGRLLRTYVDAGNRVINIADKFLEIPAFKYEITVPISDFERTRHYLEKDGFMIDDTDF
                     SSLNVKVLVRVSKDQSESFENLYNVVNKSESVMLKECNDE"
     gene            1028..1792
                     /locus_tag="PLQ55_02060"
     CDS             1028..1792
                     /locus_tag="PLQ55_02060"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPK28751.1"
                     /translation="MNKIKKENIKHIIGIVSGKGGVGKSYVTGSIAVELAKQGYRVGI
                     LDGDVTGPSIPYMFNITSGVYGDEEIIYPATTEKLGIKIISSALLLAHGEDPIMWRGP
                     LIGSLVTQFYTTVNWGELDFLLIDFPPGTSDVALSAFQTIPVDGIVLVTTPQQMVQQI
                     VKKTYKMGEEMKVKLLGVIENMAYVKLPNSDEKYYLYGQNKLEDLQNTFPINILGSLP
                     FLPQNTKLVDEGKIEVTDTTEFSAIVEKLIKELQNE"
     gene            1785..2996
                     /locus_tag="PLQ55_02065"
     CDS             1785..2996
                     /locus_tag="PLQ55_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HPK28752.1"
                     /translation="MNKYSNRRQKLYKELANNSALILFSGCEIKKSADGFYDFDVNKN
                     FFYLTGIKQEGSILVVTKIKNIVKETILTSPYDAHKEIWTGRRLKPENVREITGINEY
                     KTTDEFNALLQTLKGNVKTVYLDLEPGIFTHTGLLVSNFREEIINTVNVKVENVYPLI
                     VRLRMVKDEEEIELIRESIALTKKALDFAISKVKPGAYEYEVAAAFEYKIKAAHGVLG
                     FSTIAATGDNAVILHYPDLNTKLEAGELLLLDLGGHNNMYTADISRTVPVNGKFTPLQ
                     ITIYEIVLACNKAVIDFIKPGKTIADLQNYTREFLKERLVKAKLMDKDEDISNYYYHG
                     VSHHLGLDTHDPADRALPLEKGNVITVEPGLYFAKYGIGVRIEDNVLVTEKGSENLSI
                     SIPKEIKEIIS"
     gene            2996..4480
                     /gene="glpK"
                     /locus_tag="PLQ55_02070"
     CDS             2996..4480
                     /gene="glpK"
                     /locus_tag="PLQ55_02070"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPK28753.1"
                     /translation="MAQYILTIDQGTTSTRAMLFDKNAEIAFIAQEEIKCYYPKPGYV
                     EQDAIELWLSVLSVINQVLIKANLTFHNITAMGITNQRETTIVWDKNTGMPVAPAIVW
                     QSRQSADICNALSKHQNMIHKKTGLLINPYFSASKIRYILDNLPNGQERADAGELMFG
                     TVDSWLIYKLTNETRHVTDITNASRTMLFNINTLEWDDDILKLFNIPRSMLPEVLASA
                     DYFGETSYFHNTIPITGVAGDQQAALFGQMCLKPGDAKNTYGTGCFMLMNIGDKVQLS
                     KNGLLTTVAWKINGKVTYALEGSVFMGGASVQWLRDQLKLFKNAADTENMAYKAQQND
                     SIYFVPAFVGLGTPYWDDNARGAVFGLTREAGRHELARATLEAIAYQSRDVFGVMIKE
                     SKQDLKVLSVDGGASVNNYLMQFQADILNTTIRVPKIYETTSLGVCYLAGLHTGFFSS
                     IEAITKIHEYTRTYEPKMEEKVRERKYAGWKKAVRAVLTFSEEE"
BASE COUNT         1628 a          736 c          931 g         1435 t
ORIGIN      
        1 aataaaccgt gtatgtatgg gcgtatttta gtaaacttct taacaagagg caaaagtgtt
       61 ttagttaaga ataaaaaaac tgtaattagc gaaaatgtaa caacataggt accaaaattc
      121 ttataagcaa acgctgttac ctcatttcct tcaccgacta aggcatcgac aataaaatcg
      181 atagtaaaac tagcgaggaa taaaagtata aggctactaa aaaataatcc ataaccaaaa
      241 cccggtgatt tgattcgtga ttcttcgtgg tgattacttc cataattaag tggttcaagc
      301 atgacttcaa cttcactttg atttacttca tcgactcttt tttcttgttc ttccatacat
      361 aaagattata acatttactt attttttgcg ttagtaaagc ataaatatca taattcttga
      421 tagaattaaa ctatgggtta tgtcataaat aaaagctata ttaacgaaat cgttgtcgag
      481 aaatcacgct tcattgcgct tttatgtccg ctgactaatg agaatgattt tgatgaatac
      541 tttaaagaat caaaaacaac atataaagat gctagacatt atacttatgc ttttaggtat
      601 gggaatttag agcgcgctag tgatgatggc gaaccacaaa acacagcagg attaccgctg
      661 ctcaacttac taaagaatca tgatttagtg aatacagcgc ttattgtaat ccgttatttc
      721 ggtggtaaaa aacttggagc aggtcgttta ctgcgcacat atgttgatgc tgggaatcgc
      781 gtgattaata ttgctgacaa gtttttagaa attcctgctt ttaagtatga aataactgta
      841 ccaattagtg actttgaaag gacacgtcat tatttagaaa aagacgggtt tatgattgat
      901 gacactgatt ttagtagctt aaatgtaaaa gtgctagttc gtgttagtaa agaccaaagt
      961 gaatcgtttg aaaatcttta taatgtcgtt aacaaaagtg aaagtgttat gttaaaggag
     1021 tgtaatgatg aataagatta aaaaagaaaa tattaaacat atcatcggta ttgtttctgg
     1081 taaaggtggt gtaggtaaga gctatgtaac gggttcaatt gctgttgaac tcgcgaagca
     1141 aggctatcgt gttggaattt tagatggtga cgttacaggt ccctcaatcc cttatatgtt
     1201 taacatcact agcggggttt atggtgacga agaaattatt tatcctgcta cgactgaaaa
     1261 attaggaatt aagattattt caagcgcttt gcttcttgcg catggtgaag atccaattat
     1321 gtggcgcgga ccactgattg gtagtctagt tacacaattt tacacaactg ttaattgggg
     1381 tgaattagac tttttactca ttgatttccc accaggaaca agtgacgtcg cacttagtgc
     1441 ttttcaaact attccagttg acgggattgt gcttgtgaca acaccacaac aaatggtcca
     1501 acaaattgtg aagaaaacat ataagatggg ggaagaaatg aaagtcaaac ttctcggtgt
     1561 tatcgaaaat atggcatatg ttaaattacc aaatagtgat gaaaagtatt atttatatgg
     1621 tcaaaataaa ttagaagact tacaaaacac attcccgatt aatattttag gatcacttcc
     1681 tttcttacca caaaatacaa aacttgttga tgaaggtaaa atagaggtaa cagatacgac
     1741 tgaattcagt gcaattgtcg aaaaattaat aaaggagttg caaaatgaat aaatatagta
     1801 atagacgcca aaaattatat aaagaacttg caaataatag tgcgctcatt ctttttagtg
     1861 gttgtgagat taagaaaagc gccgatgggt tttatgattt cgatgtaaat aagaacttct
     1921 tttacttaac tggtattaaa caagaaggtt caattttagt cgtaacgaaa attaaaaaca
     1981 tcgtgaaaga aacaatttta acgagtccat atgatgccca taaagaaata tggacaggtc
     2041 gccggttaaa accagaaaat gtaagagaaa taactgggat taacgaatat aaaacaacag
     2101 atgaatttaa tgcattgcta caaacattaa aaggtaatgt aaaaacagtt tatcttgatt
     2161 tagaacctgg cattttcacg catactggtt tattagtaag caatttcaga gaagaaatca
     2221 ttaataccgt taatgtaaaa gttgaaaatg tctacccgtt aatcgttcgt ttgcgcatgg
     2281 taaaagatga agaggaaatt gaacttattc gcgaaagcat cgctttaact aaaaaggcac
     2341 ttgattttgc cataagtaaa gtaaagcctg gcgcttatga atatgaagtt gccgctgctt
     2401 ttgaatataa aattaaagcg gcgcatggtg ttttgggctt tagtactatc gccgctacag
     2461 gtgataacgc ggtaattctt cattaccctg acttaaacac taaacttgaa gcaggggaat
     2521 tattactcct tgatttaggt ggtcataata atatgtacac cgccgatata tcgcggactg
     2581 tgcccgttaa cggtaaattt actccgttac aaatcaccat ttacgaaatc gttttagcat
     2641 gtaataaagc agtgattgat tttattaaac ctggtaaaac tatcgctgac ttacaaaact
     2701 atacacgcga atttttaaaa gagcgcttag tgaaagcaaa attaatggat aaagacgaag
     2761 atatcagtaa ctactactat cacggtgtta gtcatcattt aggactcgat acgcatgatc
     2821 ctgctgatcg cgctttacca cttgaaaagg gtaatgtaat taccgttgaa ccaggacttt
     2881 acttcgcaaa gtacggtatt ggtgtccgca ttgaggataa tgtattagtg acagaaaaag
     2941 ggtcagaaaa tttatcgatt agtattccaa aagaaataaa ggagattatt agttaatggc
     3001 acaatatatt ttaactattg accaaggaac aacatcaacg agagcgatgc tctttgataa
     3061 gaatgcggaa attgctttta tcgcccaaga agaaattaag tgctactatc caaaaccagg
     3121 atatgttgaa caagatgcga ttgaattatg gcttagtgtc cttagtgtta ttaaccaagt
     3181 cttaattaaa gcgaatttaa catttcataa cattacggcg atgggaatta caaatcaaag
     3241 ggaaacgacg attgtatggg acaaaaacac tggaatgcct gtagcgccag ccattgtgtg
     3301 gcaaagtcgg caaagcgctg atatatgtaa tgctttaagt aaacatcaaa atatgattca
     3361 taaaaaaaca ggcttactta ttaaccctta ttttagcgct agtaagatcc gttatattct
     3421 tgataattta ccaaacggac aagaacgcgc tgatgctggt gaacttatgt ttggtactgt
     3481 tgactcatgg ttaatataca aattaacgaa tgaaacacgg catgttactg atattactaa
     3541 tgcctcacgg acgatgttat tcaatattaa tactttagaa tgggatgatg acatcttaaa
     3601 gttatttaat attccgcgta gtatgttacc tgaagtctta gctagtgctg actatttcgg
     3661 tgaaacaagt tattttcata atacaatccc gattactggc gtagcgggtg accaacaagc
     3721 agcattattt gggcaaatgt gtttaaagcc aggtgatgcc aaaaatacat atggaacagg
     3781 gtgctttatg ctgatgaata ttggggataa agtgcaactt tctaaaaacg gattactcac
     3841 aacagttgcg tggaagataa atgggaaagt gacttatgca ctagaaggta gtgtctttat
     3901 gggtggcgct agtgtgcagt ggctacgtga tcaactaaaa ctttttaaaa atgccgctga
     3961 tactgaaaat atggcttaca aagcacagca aaatgatagc atttactttg ttccagcttt
     4021 tgttgggtta gggactccat attgggacga taatgctcgt ggtgccgttt ttggcttaac
     4081 gcgcgaagca ggacgtcacg aattagcgcg cgcaacactt gaagcgattg cctatcagtc
     4141 tcgtgatgtg tttggtgtga tgattaaaga atcaaaacaa gacttaaaag ttttaagtgt
     4201 cgatggtggt gcgtctgtta ataattactt aatgcaattc caggctgata tattaaacac
     4261 aacaattcgt gttccaaaga tttacgaaac aacttcactt ggtgtttgct atctagcggg
     4321 tctacacacc ggcttcttca gttcaatcga agcaattaca aagattcatg agtacacacg
     4381 aacttatgaa ccaaaaatgg aggagaaagt tcgggaacga aaatatgctg gatggaaaaa
     4441 ggctgtacgc gctgtattaa ccttcagcga agaagagtaa cattaaacta ttaattacgc
     4501 tggttaataa gccagcgttt tttatttctt ctaagttata ttaacaaaag ttaataaaac
     4561 tgtagaaaaa gtattggggg tcttaactta ctttgctata atcaaatata tgaatgattt
     4621 ggttaagcgt ttaaaaggct atgactatgt tattgttgat tatgcgacaa aacttgaact
     4681 tttaaagctg cgtataagaa atccactttt aacttttaaa atatttacag
//
LOCUS       DAOPCD010000050         4730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_15068_length_4730_cov_3.121070, whole genome shotgun sequence.
ACCESSION   DAOPCD010000050 DAOPCD010000000
VERSION     DAOPCD010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15068_length_4730_cov_3.121070"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..423)
                     /locus_tag="PLQ55_02075"
     CDS             complement(151..423)
                     /locus_tag="PLQ55_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HPK28754.1"
                     /translation="MSMTNLNIRMDKKVKEEAEAIFQELGLNMTTAVNIFLRTTIREK
                     GIPFHLSLSVSNVTTKAAIEEGRKIALTDNVKGYDTVSDLKAALED"
     gene            644..1420
                     /locus_tag="PLQ55_02080"
     CDS             644..1420
                     /locus_tag="PLQ55_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK28755.1"
                     /translation="MQPILEVKNLVKTFKLSKKQQKIEKTNTKVRVAVNDLSFNVYEG
                     EIYGLLGPNGAGKTTTLRIISTLIKKDNGEVKVNGYDISKEQSKVRSQIGFLTSELKL
                     DDFFTPNYLFDYFSHLHGVPEDVIEKRKQELFDKFGISEFKEVKVSDLSSGMKQKVSL
                     VISVVHDPKFIIFDEPTNGLDVITAKTVIDFLVALKEAGKTVILSTHIFSLVERICDR
                     VGVIIDGALIFEEPMSTFSEPNSLEERFFLEYAKVKGDLA"
     gene            1420..2568
                     /locus_tag="PLQ55_02085"
     CDS             1420..2568
                     /locus_tag="PLQ55_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPK28756.1"
                     /translation="MKNLWTVIKKELKRFFTDKRMLAALILPGVIVYILYSIMGNVMQ
                     STMEGKDHEYVIRVENRPNDPRFLAIETLIADPEVGFKKVTFLDETPEDFEAMIAEKK
                     LDLYVVYNTDFLAKYNANDPNDPPVVTFHYDESVIPSLNAYSTYAGLLNASLQTYVAV
                     PINHSAEGGIDTTIIKGLLPFLLITFIFTGAMAVAPESIAGEKERGTIATLLVTPVKR
                     STLALGKVTALSIVALVSAASSFLGVMLSLPKMMGSELSLATFTAGTYLAIFAVLIVS
                     MLVFIVLNSIISAYAKSIKEASGLAAPLMMVIMAVGVTTMFGSAATNNALYLIPIYNS
                     VNILLSLFGGEFLVVPFILTMLSNLLLTAGGVYLLTVMFSNEKIMFRK"
     gene            complement(2635..3153)
                     /gene="nrdG"
                     /locus_tag="PLQ55_02090"
     CDS             complement(2635..3153)
                     /gene="nrdG"
                     /locus_tag="PLQ55_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019000008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPK28757.1"
                     /translation="MTKKKIRIAGYVNDSLVDGPGIRFTIFTQGCPYHCLGCHNPDTW
                     DPKGGEEVEISTLIAKWRKNSLLDGITLSGGEPLLQKEALLPLINAARKDGLNITMYT
                     GSTYEELIHRNDPLVNEILSKVDYLIDGPFILHELNLNLLYRGSNNQRIIDLRKTTPN
                     NIVLINTMHDFK"
     gene            complement(3146..>4730)
                     /locus_tag="PLQ55_02095"
     CDS             complement(3146..>4730)
                     /locus_tag="PLQ55_02095"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HPK28758.1"
                     /translation="FLGIMQNEWAGAQAFSSFDTYLAPFVKADNLTYAEVKQAIQSFV
                     FGVNTPSRWGSQAPFSNITLDWTVPSDLADRPAIVGGKDMPFKYKDCQKEMDMINKAF
                     LEVMIEGDANGRGFQYPIPTYSITRDFSWEETENNKLLFEMTAKYGTPYFSNYINSDM
                     EPSDVRSMCCRLRLDLRELRKKGGGFFGAGSNTGSVGVVTLNMPRLAYLSRTEVEFFN
                     ELDKLMDISARSLKVKRDVVSKLLEDGLFPYTKRYLGNFDNHFSTIGLVGMNEACLNA
                     RWLGKDLGDPESQAFTIKVLNHMREKLSDYQEAYGDLYNLEATPAESTAYRFAKHDKV
                     RYPDIITARLGEDGDPFYTNSSHLPVGYTASISEALDIQDEMQTLYTSGTVFHTFLGQ
                     KLPNWQTAARLVRKIAENYRLPYFTMSPTYSICPTHGYLTGEVEICPECKRTTEVYSR
                     ITGYYRPLKNWNDGKKAEFENRLEYADFTIEGGTVHNPKFNAPKVEVKDTADAPIKEQ
                     KPTPHKGAPIIFLNKQNND"
BASE COUNT         1432 a          924 c          857 g         1517 t
ORIGIN      
        1 ccaacggtca ttgagttgca gtcaattgcc ttaccacttg gctatggaag cgctactcaa
       61 ttataacaaa aagatttaat tattaaaact atgataaaaa gcgtactaca attaaacacc
      121 agattactaa atttaaattg attttaaaca ttaatcttcc aatgctgcct ttaaatcaga
      181 aacggtatca taccccttaa cattatcagt aagcgcaatc tttcttcctt cttcaattgc
      241 cgctttagtg gtaacatttg aaacacttaa acttaaatga aatgggatgc ctttttcacg
      301 aattgtcgtc cgtaaaaata tgttaacagc agtcgtcata ttgagtccca attcttgaaa
      361 tatcgcttct gcttcttctt ttactttctt atccatccta atatttaaat tcgtcatgga
      421 catactttaa ttcctcctat tcaatgtcat tttattatta tatcattaca ttgtcaatat
      481 gttgtcatta ttttattaat taaattaagc gtttaataat taatcccact attaataaat
      541 acaatattac ttcgcctaat ttctgtaaaa atctcgtaaa taattgtaat aacgtcaaaa
      601 tccaatatat tctttatgta taataaagaa atgaggagga actatgcaac caatcttaga
      661 agttaaaaat ttagtaaaga cctttaaact ttcgaaaaaa caacaaaaaa tcgaaaaaac
      721 aaatacgaaa gttcgtgttg cggttaatga cctctccttt aatgtttatg aaggagaaat
      781 atatggttta cttggaccaa atggcgcagg taaaacaaca acactacgca tcatctcgac
      841 cttaattaaa aaagataacg gtgaagttaa agttaatggt tatgacatta gtaaagaaca
      901 aagtaaagtt cgcagtcaaa ttggcttttt aacgagtgaa ttaaagttag acgacttctt
      961 cactcctaat tacttatttg attactttag tcatcttcac ggtgttcctg aagatgtgat
     1021 tgaaaaacgt aaacaagaac ttttcgataa attcggcatc agtgaattta aagaagtaaa
     1081 agtaagtgac cttagtagcg ggatgaaaca aaaggtttca cttgtcatca gtgtcgttca
     1141 tgatccaaaa ttcattatct ttgatgagcc gacaaacggc cttgatgtta ttactgcgaa
     1201 aacagtcatt gatttccttg tcgcgctgaa agaagcagga aagactgtta ttctttctac
     1261 gcacatcttc tcactcgtcg aaagaatatg tgaccgtgtc ggtgtcatta ttgatggggc
     1321 gttaatcttt gaagaaccaa tgagcacatt tagtgaacct aattcccttg aggaacgctt
     1381 cttccttgaa tatgcgaaag tgaaaggaga ccttgcctaa tgaaaaactt atggactgtc
     1441 attaaaaaag aattaaagcg cttcttcact gataaaagaa tgctagcggc cttaatcctt
     1501 cctggtgtta tcgtgtatat tctctattca attatgggaa atgtgatgca atcaacaatg
     1561 gaagggaaag accatgaata cgtcatccgt gtcgaaaata gaccaaatga cccgcggttc
     1621 ctcgcgattg aaacactgat cgctgatcct gaagtcgggt ttaaaaaagt tacgttcctt
     1681 gatgaaactc ctgaagactt tgaagcaatg attgcggaaa agaaacttga cttatatgtt
     1741 gtttataaca ctgatttttt agcaaaatat aacgcgaatg atcctaatga tccgccagtt
     1801 gttacttttc actacgatga atcagtgatc ccaagcttaa atgcttatag cacttatgct
     1861 gggctcttaa atgcgtccct ccaaacttat gttgccgttc ccattaatca ttcggcagaa
     1921 ggcgggattg atactacaat tattaaaggg ctgctcccct tcttacttat tacctttatc
     1981 tttaccggtg caatggcggt tgctcctgaa agtattgctg gcgaaaaaga acgcggtacg
     2041 attgcaaccc tcttagttac accagtgaaa aggagtactt tagcgcttgg aaaagttaca
     2101 gctttaagta tcgtcgccct tgtcagcgct gcaagtagtt tccttggagt aatgcttagc
     2161 ttaccaaaaa tgatggggag tgaacttagt ctagcgacgt ttaccgctgg tacttactta
     2221 gctatctttg ccgtcttaat tgtctcgatg ctcgtcttta ttgtattaaa tagcatcatc
     2281 agtgcgtacg ctaagtcgat taaagaagct agcggactag cagcaccact catgatggta
     2341 attatggcgg taggggtcac cacgatgttt ggtagtgcgg caacgaataa cgcgctctac
     2401 ttaatcccaa tttataactc ggttaatatt ttactctcac tctttggtgg tgaattcctc
     2461 gttgtaccgt ttatcttaac aatgctttct aacttacttt taactgccgg cggagtttac
     2521 ttgcttaccg tcatgtttag caatgaaaaa ataatgttta gaaaatagaa aaaacacttt
     2581 ataaacaaaa ggagtgacgc gtctcttgtc actccttttt cattatggct ttctttactt
     2641 aaagtcatgc attgtattaa taaggacaat gttattaggt gttgtttttc ttaaatcaat
     2701 aatccgttgg ttattactgc cacgatagag taaattaaga ttaagttcat gaagaataaa
     2761 aggaccatca attaagtaat caactttact taagatttca ttgacaagtg gatcgtttct
     2821 gtgaattaac tcttcataag ttgaaccagt gtacatcgta atattaagtc cgtcttttct
     2881 cgcagcgtta ataagcggta agagtgcttc tttttgtaaa agcggttcac caccacttaa
     2941 ggtaatacca tcaagcagcg agttcttgcg ccatttagca ataagcgtac ttatttcaac
     3001 ttcttctcca cccttcggat cccatgtatc aggattatga caacctaaac agtgataggg
     3061 acaaccttgg gtaaaaatag taaaacgaat ccctggtcca tcaaccaagg agtcgtttac
     3121 atatcccgct atgcgtatct tttttttagt cattattttg tttgtttaag aagatgattg
     3181 gtgcaccttt atgtggggta ggcttttgct ctttaattgg tgcgtccgct gtgtctttta
     3241 cttcaacttt tggtgcatta aattttggat tatgcactgt gccaccttca atagtgaaat
     3301 cagcgtattc taaacgattt tcaaattccg cttttttccc gtcattccag ttcttaagcg
     3361 gacgatagta tccagtaatc cgggaataca cttctgttgt tcttttacat tctggacaaa
     3421 tttcaacttc accagttaag taaccatgtg ttggacagat ggaataagtt ggtgacattg
     3481 taaagtatgg taagcgatag ttttcagcaa ttttccgcac taagcgggct gcggtttgcc
     3541 agtttggcaa cttttgccca aggaaagtat ggaacacagt ccctgaagta taaagtgttt
     3601 gcatttcatc ttgaatatca agcgcttcac taatacttgc ggtataacca actggtaagt
     3661 gacttgagtt cgtatagaaa ggatcgccgt cttcccctaa cctagcagta ataatatcgg
     3721 gatagcgaac tttatcgtgt ttagcaaaac ggtaggctgt actttccgct ggtgttgctt
     3781 ctaagttata gaggtcacca taagcttctt gatagtcact aagtttttca cgcatatgat
     3841 taagcacttt aatcgtaaag gcttgtgatt ctggatcgcc taagtctttt cctaaccatc
     3901 tagcgtttaa acacgcttca ttcatcccca ctaaaccaat tgtagagaag tgattatcaa
     3961 agttacctaa gtagcgcttt gtatatggga agagaccgtc ttcgagtaat ttactaacga
     4021 catcacgctt cacttttaaa ctgcgggcac tgatgtccat taatttatca agttcattaa
     4081 agaattcaac ttcagtacgg gagagatagg ctaagcgtgg catgttaagt gtcacaacac
     4141 caactgaacc agtattacta ccagcaccaa agaagccgcc accttttttc cgtaattcac
     4201 gtaagtcaag ccgtaagcga cagcacatac tccgcacatc acttggttcc atatcactat
     4261 taatatagtt actaaagtaa ggagtaccat acttcgccgt catttcaaat aatagcttat
     4321 tgttttctgt ttcttcccat gagaagtcac gcgtaatgga atatgttggg ataggatact
     4381 ggaatcctct tccgttagcg tcaccttcaa tcattacttc aaggaacgct ttattaatca
     4441 tatccatttc tttttgacag tctttatatt taaagggcat atctttccca ccgacaatcg
     4501 ctggacgatc agcgaggtcg ctagggacag tccagtctag tgtaatatta ctaaacggtg
     4561 cttgactccc ccaacgactc ggtgtgttga ccccaaacac aaatgattga atagcttgtt
     4621 ttacttcagc gtaagttaag ttatcagctt ttacaaatgg tgcaaggtaa gtatcaaagc
     4681 tactaaaagc ttgagcccca gcccattcgt tttgcatgat gcctaagaaa
//
LOCUS       DAOPCD010000051         4715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_15127_length_4715_cov_3.798283, whole genome shotgun sequence.
ACCESSION   DAOPCD010000051 DAOPCD010000000
VERSION     DAOPCD010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15127_length_4715_cov_3.798283"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..367
                     /locus_tag="PLQ55_02100"
     CDS             26..367
                     /locus_tag="PLQ55_02100"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HPK28759.1"
                     /translation="MVGTMLFIIPGIIFYMMYVQAYRIMIEKPEIGIMDALRESKEMM
                     NGYKMDYFVFQLSFIGHFLLIMITFGLWVIYLMPYFVVADTNYYLHLKQVRNEAKVVA
                     EAAPVEAEVVE"
     gene            458..1345
                     /locus_tag="PLQ55_02105"
     CDS             458..1345
                     /locus_tag="PLQ55_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HPK28760.1"
                     /translation="MNKKQLEIMRSGKGFIAALDQSGGSTPKALRTYGVPETAYKTDE
                     EMFTKVHEMRTRIIKSDAFNSERILGAILFENTMDRKIDASYTADYLWEEKGIVPFLK
                     IDQGLCEPISGVQLMKPMPNLASLLERAKERKIFGTKMRSVIKEANEQGIKLIVEQQF
                     AVAKKVINYGLVPIIEPEVDINSPTKKEAETILLKFILEELAKLGPNDLVMFKVTLPT
                     MMNLYAPLLKDPRVVRVVALSGGYTLEHANDLLYLNKGVIASFSRALVADLRVDQSDK
                     DFDKTLAKAVESIYEASLT"
     gene            1366..2208
                     /locus_tag="PLQ55_02110"
     CDS             1366..2208
                     /locus_tag="PLQ55_02110"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HPK28761.1"
                     /translation="MNDLQKVLLENLKEFIRVCEKHNLRYYLVTGSALGAVRHKGFIP
                     WDDDIDVAMPRNDAMKLLALKDEFKEPYFLQHYTTDPSFPYPFMKLRNSATTFEEGLL
                     KFHKINHGIYIDIFIIDGMSKTSTLKNKWKNALMWPVMYLIYAANFWQKPRKNTFILQ
                     ILLYLVSILLLPFKINNWLIKALDKSMAKIPFEEATLVGAYLLWGGNKEAMDKRIYGP
                     GVKMKFETIEAVLPENYDAYLINRYGNYMELPPEHKRVGHHKPSALSTKVSYKDYYKR
                     KKRR"
     gene            complement(2234..3241)
                     /locus_tag="PLQ55_02115"
     CDS             complement(2234..3241)
                     /locus_tag="PLQ55_02115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK28762.1"
                     /translation="MRILLFNKKQKLYDKSGVGRAMKHQVKALTEAKIPFTTNEKDDY
                     DLVVINTIDPSAFRMARKARRQGKKVIVYGHSTKEDFMNSFMLSNQIAPFFKIHLKRI
                     YKMGDLVITPTPYSKKLLDTYGLNREVVALSNGVDLARFNADNKEKIQKFREYFKLKP
                     DQKVVMTVGLFLARKGLHDLIEIAKDRPEYTFIWFGHTPKAVLSRKIKKAIKNKPDNV
                     IMPGYIAGDIIEGAFANADVFFFPTYEETEGIVVLEALASKCPLLIRDIPIYEEWLTH
                     GVNCHKGKTNEEFKALLDDIITKKAADTREAGYKVAAERDIKEIGKKLGEIYRRVLEE
                     K"
     gene            3379..4509
                     /locus_tag="PLQ55_02120"
     CDS             3379..4509
                     /locus_tag="PLQ55_02120"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HPK28763.1"
                     /translation="MREAYKKIPCRITVIDLLKLTGMIFICIPHCLQRWGPSDYTSTL
                     GFSFFYSVGLSMFFFSSGIVLRRITSLKDLLIFIVKTVGVYLTSAFIFTVLSIYTLPR
                     FTLLEKDFGYWMNELYLRTDTFYWYLLVATLINIFIASAYYFVSLIKKNNLKWDIFKA
                     SLIIILTVAYSMIFLHIYNREDLGPGCLGASLVLYYLPITLFGFLFNIFKPYLLKLPR
                     QELLKGIATLVALVIYIPLLIVYRDWLPHLNGSFWTISALWICSLSGTIVYYYLLSKI
                     SHFTIAKKVSYYGFFSGQFYLVHVYIIRLFATYIPRPETFDFPAIMFVTFLTIIFTIG
                     SILITVFLRFIPYTDFLLFISYDRYKDIIYPHRWLKRVVLQK"
BASE COUNT         1476 a          772 c          840 g         1627 t
ORIGIN      
        1 ttatttctaa acaatgctca tcgtgatggt aggtacaatg ttatttatca ttcctggcat
       61 catcttctat atgatgtatg tccaagccta tcgtatcatg attgaaaaac cagaaattgg
      121 tattatggat gcgcttcgtg aaagtaaaga aatgatgaat ggctacaaaa tggattattt
      181 tgtgttccaa cttagtttca tcggtcactt cttattaata atgattacct ttgggctatg
      241 ggtaatttat ttaatgccat actttgttgt tgccgacact aattattact tacaccttaa
      301 acaagttcgt aatgaagcaa aagtcgtcgc cgaagctgca ccagttgaag ctgaagtagt
      361 cgaataattt ttaaaaatta aaactctcct gtgctaaaac atatgtaatt taattatttt
      421 cctttacaat atgtatagat agaggggagt ttttattatg aataaaaaac aattagaaat
      481 tatgagaagc gggaaagggt tcattgccgc tcttgatcaa agcggaggtt caacaccaaa
      541 agcattacgc acctatggtg tccctgaaac cgcatataaa actgacgaag aaatgtttac
      601 taaggtccac gaaatgcgga cgcgaattat aaaaagcgat gcctttaata gtgagcgaat
      661 tttaggggca atcttatttg aaaatacaat ggaccggaag attgatgcaa gttataccgc
      721 tgattactta tgggaagaaa aaggtattgt tccttttctt aaaattgatc aaggtctttg
      781 tgaacctata agtggtgtcc aactgatgaa gccaatgccg aatcttgcta gtcttttaga
      841 aagagcgaaa gaacgaaaaa tatttggtac taaaatgcgt tcggtcatta aagaagcgaa
      901 tgaacaaggc attaaactta ttgtcgaaca acaatttgcg gtcgctaaaa aagtaataaa
      961 ttacggttta gtgccaatta ttgaacctga agtggatatt aatagtccaa cgaaaaaaga
     1021 agcagaaacg attcttctaa aatttatcct cgaagagtta gcaaaattag gaccaaatga
     1081 tttagtgatg tttaaagtga ctctacctac aatgatgaat ttatatgcac cacttttaaa
     1141 agatccgcgt gttgttcgtg ttgtagcttt aagcgggggt tatactcttg agcacgctaa
     1201 tgatttactt tatttaaata aaggtgtaat tgctagtttc tcacgggcat tagtcgcgga
     1261 tttacgcgtc gaccaaagtg ataaagactt tgacaaaact ttagcgaagg ccgttgaatc
     1321 aatttacgaa gcttcgctta cctaaataag gaaaagacat taaggatgaa tgatttacaa
     1381 aaagtattat tagaaaatct aaaagagttt attcgtgttt gtgaaaaaca taatttacgc
     1441 tattacttag taacagggag tgcactaggt gccgtaagac ataaaggctt tattccgtgg
     1501 gatgatgata tcgatgtagc gatgccacga aatgatgcga tgaaacttct cgcgctaaaa
     1561 gatgaattta aagaaccata cttcttacag cactacacaa ctgaccccag ttttccttat
     1621 ccgtttatga agttacgcaa tagcgcgaca acttttgaag aaggtctttt aaagtttcat
     1681 aagatcaatc acggtattta tattgatatt tttattattg atgggatgag caagacatca
     1741 acccttaaaa ataaatggaa aaacgcttta atgtggccag tgatgtatct tatttatgcc
     1801 gcgaatttct ggcaaaaacc gcgtaaaaat acatttatcc ttcaaatctt actctatctt
     1861 gtatcaattc ttttattacc atttaaaatt aataattggc tcattaaagc acttgataaa
     1921 agtatggcta aaatcccttt tgaagaggcg acactcgttg gtgcttacct gctttggggc
     1981 gggaataaag aggcgatgga taaacggatt tatggtccag gtgttaagat gaaatttgaa
     2041 acaattgaag ccgttctccc tgaaaattat gatgcttatt taataaatcg ttatggtaat
     2101 tacatggaat taccaccaga acataaaaga gtcggtcacc ataaaccgag cgccttaagc
     2161 actaaagtta gttataaaga ttattacaaa cgaaaaaagc gccgctaggg cgcctttttt
     2221 gtcgtttagt taactatttt tcttcgagta ctcggcgata aatttctccg agttttttac
     2281 cgatttcttt aatgtcacgt tctgctgcta ctttgtaacc tgcttccctt gtatccgcgg
     2341 cttttttagt aataatgtca tcaagtaacg ctttaaattc ttcattagtt ttacctttat
     2401 gacaattgac accgtgcgtt aaccattctt cgtaaatcgg gatatcacgg atgagaagtg
     2461 gacacttact tgcgagggct tcaaggacaa caattccttc tgtttcttcg taagttggga
     2521 agaagaagac atcagcgttt gcaaaagcgc cttcgataat atcaccagca atgtagcctg
     2581 gcataattac attatcgggt ttatttttaa tggctttttt aattttacga cttagaaccg
     2641 ctttaggggt atgtccaaac caaataaatg tatattcagg ccgatctttt gcaatttcaa
     2701 ttaagtcgtg gagaccttta cgcgctaaga aaagcccaac agtcattacg actttttggt
     2761 caggtttaag cttaaaatat tcacgaaact tttgaatttt ttctttatta tcagcgttaa
     2821 atctcgctaa atcgacaccg tttgataagg caacaacttc acgatttaag ccgtatgtat
     2881 caagtaattt tttggagtat ggtgttggtg ttatgacaag gtcacccatt ttgtaaatgc
     2941 gttttaagtg tattttgaaa aaaggtgcaa tttgattact tagcatgaag gagttcataa
     3001 agtcttcttt tgttgagtga ccataaacta ttactttttt tccttgtctt cttgctttgc
     3061 gtgccatacg gaaagcacta gggtcaatgg tgttaatgac gacgagatca tagtcatctt
     3121 tttcatttgt ggtaaacggg atttttgctt ctgttaatgc tttgacttga tgcttcatcg
     3181 cgcgacccac accagactta tcatataatt tttgtttttt attaaataag agaattcgca
     3241 tactccgggc tcctttcact ctctttttat catatcacga tatatacttt tttgaacgtg
     3301 ttaatagttt ataatttaat gctataatta ttgctattaa agggaactaa acccttaata
     3361 atagaaagag ttaaagctat gcgtgaagct tacaaaaaga taccgtgtcg gataacggtc
     3421 attgacttat taaaacttac agggatgatt tttatttgta tcccgcactg tttacaaaga
     3481 tgggggccaa gtgattatac atcaacatta ggcttttctt ttttctatag tgttggtctt
     3541 tcgatgtttt tctttagtag cggaattgtg ctccgccgca tcacatcact taaagatttg
     3601 cttatattta tagtaaaaac tgttggtgtg tatttaacat ccgcctttat ttttactgtt
     3661 ttatcaattt atactttgcc acgctttacg cttttagaaa aagactttgg ctactggatg
     3721 aatgaattgt atttacgcac tgatacattt tattggtatt tgcttgtcgc aacattaatt
     3781 aatattttta tcgctagtgc atattacttt gtgtctttaa taaagaaaaa taaccttaag
     3841 tgggacattt ttaaggcaag tcttataatt atacttacag ttgcttactc aatgattttt
     3901 ttacatattt ataatagaga agacttagga ccagggtgct taggtgcttc tttagtgctt
     3961 tactatttac cgataacttt atttggtttt ctttttaaca tatttaaacc gtatttactt
     4021 aaactaccac gccaagaatt acttaagggt atcgctactt tagttgcgct agtaatttat
     4081 ataccgcttt taattgtgta tcgcgattgg ctccctcatt taaacggctc attttggaca
     4141 attagtgcct tatggatatg ttcacttagc gggacaattg tgtattatta cttactttcc
     4201 aaaatctcac actttacaat tgctaaaaaa gtatcatatt acggcttctt tagcggacaa
     4261 ttttatttag tgcatgtgta tattattcgt ctttttgcta catatattcc gcgacccgag
     4321 acgtttgact ttccggcaat tatgtttgtt acctttttaa caattatctt cacaatcggt
     4381 agtattttaa ttactgtttt cttacgcttt attccttaca cggacttctt attatttatt
     4441 agttatgatc gctataaaga cattatttat ccgcatcgtt ggctaaaaag agtagtgctc
     4501 caaaaataaa atttaatgat ttaattaaga agtgctacgc tgatagtcgt caattaataa
     4561 tgaaaaaatg tttcaaccgt gaataaaaat ttgctgtgtt tattttactt tgcaaaataa
     4621 ataatcataa attacaaaaa gtgtaattct aaaacatttt tgttacatga aaaagataaa
     4681 gtgacaaaat agaaaataaa gttttaaaaa tagat
//
LOCUS       DAOPCD010000052         4664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_15320_length_4664_cov_3.385767, whole genome shotgun sequence.
ACCESSION   DAOPCD010000052 DAOPCD010000000
VERSION     DAOPCD010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15320_length_4664_cov_3.385767"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..729)
                     /locus_tag="PLQ55_02125"
     CDS             complement(85..729)
                     /locus_tag="PLQ55_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPK28764.1"
                     /translation="MGKLFNEEVASQIKGVLAQMKNEITIKLFIDGKCDTCLETKQLL
                     EETKTLSDKINLVVHEINESKEEAEKYDITLTPTFIILDDKGTYSGVRFSGFPGGYEI
                     NSFVSSLLEMSGLRHEFPQEVVDRVAKIKEPIDIKVFVTLGCPHCPGAVETAHRLAML
                     NSNIKATMIESQTFMELARTHKVSSVPKIVFNDALDLLGNQPVEAFLDEIETLL"
     gene            complement(740..1699)
                     /locus_tag="PLQ55_02130"
     CDS             complement(740..1699)
                     /locus_tag="PLQ55_02130"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPK28765.1"
                     /translation="MFNFQLSSNKPQINYDELYDFIALGLGPAGLNAGLYAHRKGLKT
                     LIVGHRVGGQLLNTADIDNYLGFSNIPAQKLIDEFRNHIENSKVPMLTDVLVTNIEKS
                     ADIFIITLNNGKTLQSKTVLYALGGSPRKLKVPGEDRLAGKGVAYCATCDGPFFQDKD
                     VIIAGGGNSAIDATKDLARIAKSVTIIEKFTISADKTSVEQVAKLPNVTMFEETDVTE
                     IIGDKYIEGIRVFDRKANKERIINTEGLFVEIGSIPNTALLKDLVELNKWGEVIVNNK
                     QETSLKGLYAAGDVTNNWIRQVIVAAADGAKAALEANNYITKK"
     gene            1979..3754
                     /gene="dnaX"
                     /locus_tag="PLQ55_02135"
     CDS             1979..3754
                     /gene="dnaX"
                     /locus_tag="PLQ55_02135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPK28766.1"
                     /translation="MSYKALYRTYRPLTFGEIAGQRAIVRTLQNALNQNKIAHAYLFS
                     GPRGTGKTTMAKLFAKALNCEEGLGHQCNRCTNCNSVNDGSHPDVYEIDAASNNGVED
                     VRQLIENINYAPIRGRYKVYIIDEVHMMTQSAFNALLKTLEEPPQNVIFILATTEPHK
                     VIPTILSRCQRYNFSKVSDRDITKRLKTVLETEVIPYEEEALKLLVSLADGGVRDALS
                     MLDQVLAYSRQQLKLEDVLALFALTSKLEQLNLLRAVASSNVEEVFTILSGLIDKGVD
                     VRRLTTDLLNLLKDALIYYRTENTDLLNYLRKEEVQELNMMFEIENLNMMIENILKAQ
                     ADYRFASDVKNIFEITLLKIITSLNQDTMVEKELIIQKPKVKTAPSVPKKDEKHAIVT
                     ETEKETIVEEQELPPFMTQSREKLEKRVTETKSEIVKDEVKTAQPVPEKVQYANPLHN
                     EGQVIELDDDTIIKILTLADRDEKLSLRNNRWDDLALMAMDSVVGEHANVLLNGAPYA
                     LAPEIILVEFNFDQYARYINILENQKIMQDIIERLVGRRIPVYGLTHGQAVSFARKYR
                     SMSQIQKLPRVKSVKEELEGKFDYV"
     gene            3747..4052
                     /locus_tag="PLQ55_02140"
     CDS             3747..4052
                     /locus_tag="PLQ55_02140"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPK28767.1"
                     /translation="MSSSMQKLMQQAQKMKREMQKAQEALNAKEFSVTKGGGVTVAML
                     GSKEVVEVTIDEDLFDKDNKEMVETLIIMAFNECIEQINAEEAAINEAITGSATGLF"
     gene            4052..4651
                     /gene="recR"
                     /locus_tag="PLQ55_02145"
     CDS             4052..4651
                     /gene="recR"
                     /locus_tag="PLQ55_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPK28768.1"
                     /translation="MDDLPTLNALIEHLKRLPSVGQKSAERMAQAFLTFDDETLKDFG
                     TVITKLKEKVHLCPNCGLYTEAELCSFCVDPNRNNKTLIVVSYPKDVLGFSKLEDYHG
                     RFHILGGVISPSSNKGVELLSIPELEKRIVRDNVEEIILATNPTVEGELTALFVSKKL
                     ADLPVKITRLAYGLPMGGQVDYADAMTLFKALEGRKDLK"
BASE COUNT         1551 a          793 c          830 g         1490 t
ORIGIN      
        1 ttaagtccaa aagtcaggtt atatcttctt atttatataa gaaaaaggcc cctaatagta
       61 gtaggagcct taatgtaagt gtttctataa taaagtttcg atttcgtcta agaaagcttc
      121 cactggttga ttaccaagta agtcaagtgc atcattgaac acaatttttg gaacgctaga
      181 aactttatgt gtacgcgcta actccataaa tgtttgtgat tcaatcattg ttgctttaat
      241 gttactattt aacattgcta aacgatgagc ggtttcaaca gcacctggac agtgcggaca
      301 acctagagtc acgaatactt taatgtcaat tggctcttta attttagcaa cacggtccac
      361 tacttcttgt gggaattcgt ggcgtaatcc agacatttct aaaagtgaac taacgaatga
      421 attaatttca taacctcctg gaaaaccact gaatctaact ccgctatatg ttcctttatc
      481 atcgagaatt ataaatgttg gagtaagcgt aatatcatat ttttccgctt cttcttttga
      541 ttcgttaatt tcgtgaacaa caagattgat tttgtcgctt aatgttttag tttcttctaa
      601 tagttgtttt gtttctaaac aagtgtcaca tttgccatca ataaataatt tgattgtaat
      661 ttcatttttc atttgtgcaa gcacgccttt aatctggctt gcaacttctt cgttaaataa
      721 tttccccata atttctcctt tacttttttg taatatagtt gtttgcttct aaagcagctt
      781 tggctccatc ggctgcagca acgatgactt gacgaatcca gttattagtt acatctcctg
      841 ctgcatatag acctttaaga cttgtttctt gtttgttatt aacaattact tcgccccact
      901 tgtttaattc aacaaggtct tttaatagag cggtatttgg aatactccca atttcgacaa
      961 ataagccttc agtgttaata attctttctt tatttgcctt tctgtcgaac actctgattc
     1021 cttcaatata tttatcacca atgatttctg taacatcagt ttcttcaaac atcgttacat
     1081 ttggtaattt tgccacttgt tctacactag ttttatcagc gcttatcgtg aatttttcaa
     1141 taattgtcac actttttgca atacgcgcta agtctttcgt ggcgtctatc gcactattac
     1201 ccccacctgc gattattaca tccttatctt gaaagaatgg tccgtcacac gtagcgcagt
     1261 aagcgactcc tttaccagca agtctatctt caccaggcac ttttagcttg cgcggactac
     1321 ccccaagagc atataacaca gttttacttt gtaaagtctt gccgttattg agtgtaataa
     1381 tgaatatatc agcacttttt tcaatgttag taactaacac atcagtgagc attggtactt
     1441 ttgaattttc aatatgattt ctaaattcat caattaattt ttgtgcggga atatttgaga
     1501 aacctagata attatcaata tcagcagtat ttaataattg accgccaaca cgatgaccaa
     1561 ctattagagt ttttaagccc ttgcgatgag cgtataatcc agcatttaga ccagctggac
     1621 ctaatccaag agcaataaaa tcatagagtt catcataatt aatttgaggt ttatttgagc
     1681 ttagttggaa attgaacata atatagtttt ctcctttctg ccgatattat ataggtcgat
     1741 taacttaaat aaaaacaatt tgcttaatgc aaaaagtgtc gctacataaa atgcgtgttt
     1801 ttatgttttt agacaataaa atttataaca attattaata taaagaaaca ttaaactaat
     1861 tgcagaatga aacacattat gaagcaataa aaagtcatca tcctgcatat tatcaaaaca
     1921 atttaagttt tgtgttctta ttcctacaat gtagtcttaa ttattgtata attactacat
     1981 gagttataaa gcactatatc gaacatatcg gccacttact tttggcgaaa tagctggaca
     2041 aagagcaatt gtccgcactt tacaaaatgc cctaaatcaa aataagattg cacatgctta
     2101 tctttttagt ggaccgcgcg gtactggtaa aacaacgatg gctaaactct ttgctaaagc
     2161 ccttaattgt gaagagggtc ttggtcatca atgtaatcgt tgcactaatt gtaatagtgt
     2221 taacgatggc agccatcctg atgtatatga aattgacgcc gccagtaata atggcgttga
     2281 ggatgttcgt caacttattg aaaatataaa ttatgcgccg attagaggtc gttataaagt
     2341 atatatcatt gacgaagttc atatgatgac acaaagtgca ttcaatgctt tgcttaaaac
     2401 acttgaagag ccaccacaaa atgttatttt tattctggca acaactgagc cacacaaggt
     2461 aattccaacg attttaagtc gctgtcaacg ctataacttc tcaaaagtaa gtgatcgcga
     2521 cattactaaa cgtttaaaaa cagttttaga aactgaagtt attccttatg aagaagaagc
     2581 actaaaatta ttagtgtcgt tggcagacgg aggcgtccgt gatgctttaa gcatgttaga
     2641 ccaagtttta gcttactctc gtcaacaatt aaaacttgaa gatgtattag cgcttttcgc
     2701 cctaactagt aaactagaac aacttaactt attaagagca gttgctagta gtaatgtcga
     2761 agaagttttt acaatactat caggtttaat tgataaaggt gttgatgtaa gacgtttaac
     2821 aacagattta cttaatttat taaaagatgc acttatttat tatcggacag aaaatactga
     2881 tttattaaat tacttgcgca aagaagaagt gcaagaatta aatatgatgt ttgagattga
     2941 aaacttaaac atgatgattg aaaacatatt gaaggctcaa gcagattatc ggtttgcaag
     3001 tgatgtcaaa aatatctttg aaattacact tttaaaaatt atcacatcat taaatcaaga
     3061 cacaatggtc gaaaaagagt taattattca gaaaccaaaa gtaaaaactg ccccatctgt
     3121 gccgaaaaag gatgaaaaac acgctatagt tactgaaact gagaaagaaa ctatagttga
     3181 agagcaagaa ttaccacctt ttatgacaca gtcaagggaa aaactagaaa agcgcgtcac
     3241 cgaaactaaa tcagaaatag ttaaggacga agtgaaaact gcccagcctg tgcctgaaaa
     3301 ggtccaatat gcaaatcctt tacataatga gggtcaggtt attgaattag acgatgacac
     3361 tattatcaaa atacttacac tagctgaccg cgatgaaaag ttgtcactta gaaataatcg
     3421 ttgggatgat ttagcgctga tggctatgga tagtgtagtt ggtgaacacg ctaatgtatt
     3481 gcttaatggt gctccttatg ccctagcccc agaaatcatt ctcgtcgaat ttaattttga
     3541 tcagtatgct cgttatatta atattttaga aaatcaaaaa attatgcaag atattataga
     3601 aaggttagtg gggcgtcgca taccagtcta tgggcttacc catggtcaag ctgtgagttt
     3661 tgcaagaaaa tatcgctcga tgagccaaat tcaaaaatta ccgcgtgtta agagcgtaaa
     3721 agaagaatta gaagggaaat ttgattatgt ctagtagtat gcaaaaatta atgcaacaag
     3781 cgcaaaaaat gaagcgcgaa atgcaaaaag cacaagaagc attaaacgca aaggagttct
     3841 ccgttactaa aggtgggggt gtaactgttg ctatgcttgg aagtaaagaa gttgtcgaag
     3901 tgacaattga cgaagatctt tttgataaag ataataaaga aatggttgaa acgcttatca
     3961 ttatggcttt taacgaatgt attgaacaaa taaatgcaga agaagccgct attaatgaag
     4021 caatcacagg aagtgcaaca ggactatttt aatggatgat ttaccaacac taaacgcatt
     4081 aattgaacac ttgaaacgtt taccaagtgt tggtcaaaaa agtgctgagc gtatggcaca
     4141 agctttttta acatttgatg atgagacatt aaaagatttc ggcacagtta tcactaaatt
     4201 aaaagaaaag gtgcacctgt gtcctaattg tggtctttac acagaagcgg aattatgttc
     4261 tttttgcgtt gacccaaatc gtaacaataa aactttaatc gtcgttagtt atcctaagga
     4321 tgttttgggt tttagtaaat tagaagacta ccatgggcgc ttccatatat taggaggcgt
     4381 tattagtcct tcttcaaata aaggcgttga acttctttca atcccagagt tagaaaaaag
     4441 aattgtacgt gacaatgttg aagaaattat tcttgccaca aatccgacag tagaaggtga
     4501 actaacagca ctctttgtta gtaaaaaact tgcggattta cctgtaaaaa ttacacgatt
     4561 ggcttatgga ctgccaatgg gcgggcaagt agattacgct gatgctatga ctttatttaa
     4621 agcattagaa ggacgaaaag atcttaaata ggagggtgtt atgt
//
LOCUS       DAOPCD010000053         4653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_15358_length_4653_cov_2.943889, whole genome shotgun sequence.
ACCESSION   DAOPCD010000053 DAOPCD010000000
VERSION     DAOPCD010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15358_length_4653_cov_2.943889"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..>1012)
                     /locus_tag="PLQ55_02150"
     CDS             complement(22..>1012)
                     /locus_tag="PLQ55_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28769.1"
                     /translation="FDNPLILESHTKRHLAVLDSFFGDKNIVGVSPWCMHDYYTHHEF
                     GSGDKICYHGVLDIYREPKPAAYVYTSNLTSDPVLFPLFRGDNGDLPESKLYKTMVLS
                     NASYIDLYKDDEVVKRYYPRRDLYKHLPHPPFIIDTFISDNFKSTIKFSKRDRKKIAK
                     LLNYAALNNFSRLSLWQKLQLGWLIVKYRLKWDNLVELWFLNVVGWGDKSSGYTLVGY
                     NDEDIEIIRKRIENTTTTTYEIELLKNELVNTDTFDTTIVTIRSLSPYRNHYDFSPVS
                     VVVTGPLKVYGPEIQNLHAGVLNVYVRSINTTGRGTITIGVGGTIKTFNVEIK"
     assembly_gap    1013..1022
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1023..2342)
                     /locus_tag="PLQ55_02155"
     CDS             complement(<1023..2342)
                     /locus_tag="PLQ55_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPK28770.1"
                     /translation="MKHALNYNWSFIPKFNDEYIKSIPQGVISVDLPHNMVDFPFNYF
                     DEKDFDSVGTYFREIVFTKVLESDKFVLRFGGVNNSFTLNVNSQLVGTFTRPYMEENI
                     DITKYVVEGNNLIVVCVNGSEKVNAPPFGNTVDYFPFSGIYREVTLFVYNALDIIDVK
                     VTANHLGQIEVNPDIRNINKVDYSVNYEVYEGTKLIASSIEHNFTVENPKLWSVNNPN
                     LYNLVTTITANKDVKQYVSVFGFRSAVFLSDGFFLNGEYTPLVGLNRHETYPYVGAAA
                     TSFLQFYDVVKLKNLGVNYVRCSHYPPSRAFLDACDFLGLMVINEVPGWQYIGDEAWR
                     KIHLQNIETMINRDFNRPSIIAWSIRINESADHPFYEECHKLAKALDPVRATTGTRNF
                     TNSELFEDIYSYNDFSHDGTNKGLLAKRKVTKSKKPYIVSENNGHMYP"
     gene            2418..>4653
                     /locus_tag="PLQ55_02160"
     CDS             2418..>4653
                     /locus_tag="PLQ55_02160"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HPK28771.1"
                     /translation="MEYKYIHLKTNDTSYVLAINKYGHLENLYYGAKLALNEDLSILV
                     NKQTNMQGTTTVYSGEPHDTYSLDFVNLELGTIGKGDYRRPSILLEHELGFASDFVVK
                     KHGFAGREVLLVNDLPIPHGYDEYFYVILEDNISKVKVQLDYLIFKRANVIARRLVVL
                     SGKGKLTKAASLNLDLNNDHFDVFSTYGTWANEVHASRNKLVPGLFEVATSVGPSSNR
                     HNPFILLLNEKTDLHKGIGYGVNLIYSGPFKASVELTFDNRVRLQHGINDETFSKELD
                     GKPFITPFAALTFSDKGENGLSQNFHRFVIDHVVRYEYARKPRPILLNNWEATYLTFD
                     EAKIKKLMREASKLGIELMVLDDGWFINRDDDFGGLGNYEVNKKKLPRGLKGLSDYAT
                     KLGMSFGLWFEPEMVSADHPIFTEHPEWVVKIPGVKPSTGRHQYSLNLTLKEVQDYIV
                     ENVSKVLKSADIKYVKWDYNRNLSDYYSPSGEYGTFFYDYTLGLYAIMQRLTKEFPTI
                     LWEGCASGGNRFDLGILSFFDQIWVSDCSDAYERIRMHTNLAKAYPLSVMSGHVSAAP
                     NHQLLRDLPLQTRFNNAFMSGGFGYELDVNNLTPKDKKEIKAYNEIAKAFRSVIMDGK
                     YYELTLSSAKDKKAYALLSEASDNALLLISSGIHSPMPEVLKFPSIPVKQDLTYEVFA
                     LPQSTDISTFGSLLNMVLPKFIKHDGAVIKYLDKRSSAEELVKVEVIERYTVSGKALR
                     AGALT"
BASE COUNT         1481 a          817 c          895 g         1450 t
ORIGIN      
        1 ggccattact gcttctcctt tctatttaat ttcgacgttg aaggttttaa tcgtaccgcc
       61 aacaccaata gttattgtac cacgccccgt cgtgttaatg gagcggacat acacattaag
      121 tacaccagcg tgtaagtttt ggatttctgg tccgtaaacc tttaggggac cagtaacgac
      181 aactgaaaca ggactaaagt cataatgatt acgataagga cttagactac ggattgtaac
      241 aattgttgta tcaaatgtat ctgtgttcac taattcattt tttaaaagtt cgatttcata
      301 agtcgtagta gtggtgtttt cgattctttt tctaattatt tcgatatctt catcgttata
      361 gcccactagt gtgtagccgc ttgacttatc accccagccg acaacattaa ggaaccataa
      421 ttcgacaaga ttatcccatt taaggcgata cttaacaatt aaccacccga gttgtaactt
      481 ttgccagagt gagagccgac taaaattatt gagagcggca taatttaata gttttgcgat
      541 ctttttacga tcacgctttg aaaatttaat tgttgactta aagttatcac taataaaagt
      601 atcgatgata aatggtggat gcggtaaatg tttatataag tcacgccgcg ggtagtagcg
      661 ctttacaact tcatcgtctt tgtataaatc aatgtaactt gcgttactta gcaccatcgt
      721 tttatataat ttactttctg gtaagtcacc gttatcaccg cgaaaaagcg ggaataaaac
      781 agggtcactt gtaaggttag aagtatacac atacgccgcg ggtttaggtt cacgatatat
      841 atctaacaca ccgtgataac aaattttgtc accactacca aattcgtggt gcgtgtagta
      901 gtcatgcata caccacgggg aaacaccgac aatattttta tcaccaaaga aactatcaag
      961 caccgctaaa tggcgttttg tatgactttc taaaataagc gggttatcaa atnnnnnnnn
     1021 nnggggtaca tgtgcccatt attttcacta acgatgtaag gttttttact ttttgttact
     1081 ttacgcttag ctaataatcc cttattcgtg ccgtcatgac taaagtcatt ataggagtag
     1141 atgtcttcaa aaagttcgct attagtaaag ttacgtgttc ctgttgtagc gcgaacgggg
     1201 tcaagtgctt ttgctagttt atgacactct tcgtagaatg ggtggtcagc gctttcatta
     1261 atcctaatgg accacgcgat aatacttgga cgattaaagt cacgattaat catcgtttca
     1321 atgttttgta aatgaatctt tcgccacgct tcgtccccta tatactgcca tcctggaact
     1381 tcatttatga ccataagtcc taaaaaatca caggcatcaa gaaaagctct acttggtggg
     1441 tagtgcgagc aacgcacata attaacacca aggtttttaa gttttacaac atcataaaat
     1501 tgtagaaaac tcgtggctgc tgcgccaaca tatgggtaag tttcatgacg attaagtcca
     1561 actaaaggcg tgtattcacc gttaagaaag aagccgtcac taagaaatac ggcactgcga
     1621 aaaccaaaga cactaacata ttgtttaaca tctttattag cggtaatagt agtaactaaa
     1681 ttgtataaat taggattatt aacactccaa agtttggggt tttcgactgt gaaattatgc
     1741 tcgatactac tagcgataag ctttgtgcct tcgtatactt cataatttac agagtaatca
     1801 accttattaa tgtttctaat atcggggttt acttcaattt gccctaaatg attagcggta
     1861 acttttacat caataatatc taatgcgtta tatacaaaaa gtgttacttc acggtaaatt
     1921 ccgctaaagg ggaagtagtc aacggtatta ccaaatggtg gagcgttaac tttttcactg
     1981 ccgttaacac atacaacaat taaattattg ccctcgacaa catatttcgt aatatcaata
     2041 ttttcttcca tatatggtct agtaaatgta ccgaccaatt gcgaatttac atttaatgta
     2101 aaagaattat ttacgccacc gaagcgtaag acaaatttat cactttcaag taccttggta
     2161 aaaacgattt cacggaagta agtaccaaca ctatcaaaat ccttttcatc aaagtaatta
     2221 aaaggaaagt cgaccatatt atgcgggagg tcaacactta ttaccccttg cgggatagac
     2281 ttaatatatt catcattaaa ttttgggatg aatgaccaat tatagttcag tgcgtgtttc
     2341 atacatacat attttaacat taaatgataa ttacttagta aatattaaaa atagcgtatg
     2401 tatgttaaaa ttaatctatg gaatacaaat atatacacct taaaacaaat gacacatcat
     2461 atgtgcttgc cattaataaa tatggccatt tagaaaacct ttactatggc gcaaagttag
     2521 cgctaaatga agatttaagc attttagtta ataaacaaac taatatgcaa gggacgacaa
     2581 ctgtttatag cggagaaccg catgacactt attccttaga ttttgttaat ttagaattag
     2641 ggacgattgg caaaggtgat taccggcgac caagtatttt acttgaacat gaactaggat
     2701 ttgcgagtga ttttgtcgta aaaaagcacg gttttgccgg aagagaagta cttttagtaa
     2761 atgatttacc aattccgcat ggctatgatg aatactttta tgtcatcctt gaagacaaca
     2821 ttagtaaagt aaaagtacaa ttagattact taatttttaa gcgcgctaat gtgatcgctc
     2881 ggcgtcttgt tgtccttagc ggaaaaggta aattaacaaa agcagcaagt ttaaacttgg
     2941 atttaaataa tgatcatttt gatgtgtttt caacttatgg cacatgggca aatgaagtcc
     3001 atgcttcgcg taataagtta gtgcctggtc tctttgaagt ggcgacaagt gttggcccaa
     3061 gtagcaatcg tcataatccg tttattttac ttttgaacga gaaaactgat ttacataaag
     3121 ggataggtta tggtgttaat ttaatttata gcggaccatt taaagcttcg gtagaattaa
     3181 ctttcgataa tagggtcagg cttcagcacg gcataaatga tgaaacattt agtaaagaac
     3241 ttgatggaaa gccttttatt accccgtttg ccgcccttac atttagtgat aaaggcgaaa
     3301 atggccttag tcaaaacttc catcgctttg ttatagacca tgtcgtgcgt tatgaatatg
     3361 cgcggaaacc gcgtccaatc ttacttaata actgggaagc aacttatctt acatttgatg
     3421 aagcaaaaat taaaaaacta atgcgtgaag caagtaaatt agggattgaa ctaatggttt
     3481 tagatgacgg atggtttatc aatcgtgatg atgactttgg cggattaggt aattacgaag
     3541 taaataagaa gaagttacca cgcggcttaa aaggcctaag tgactacgca acgaaattag
     3601 gaatgtcctt tggtttatgg tttgaaccag aaatggttag tgctgatcac ccgatattta
     3661 ccgaacaccc tgaatgggtt gttaaaattc ctggtgttaa acctagtact ggacgccatc
     3721 agtattccct taatttaacg ctcaaagaag tccaagacta tattgtcgaa aatgtgagta
     3781 aagtattaaa gagtgccgat atcaaatatg ttaagtggga ttataatcgt aacttaagtg
     3841 attactattc accatcgggt gaatatggca cattctttta tgattatacg cttggcttat
     3901 atgcgattat gcagcggtta accaaagaat ttccaacgat tttatgggaa ggttgtgcaa
     3961 gtggcggtaa tcgttttgat ttaggaattt tatcgttctt tgatcaaatt tgggtgagtg
     4021 actgtagtga tgcttatgaa cggattagaa tgcataccaa tctcgcgaaa gcttatccgc
     4081 ttagtgtcat gtctgggcat gttagtgctg cccctaatca tcagttacta cgtgatttac
     4141 cactgcaaac acgctttaat aatgccttta tgtctggtgg ttttggctat gaattagatg
     4201 tgaataattt aacgccaaaa gacaaaaaag aaattaaagc atataacgaa attgctaaag
     4261 cattccgcag tgttattatg gacgggaagt attacgagtt aacacttagt agcgctaaag
     4321 ataaaaaggc gtacgcacta ctaagtgaag ctagcgataa tgcactcctc ttaattagta
     4381 gcggcattca ttcaccgatg ccggaagttt taaagtttcc gtctattccc gtgaagcaag
     4441 acttaactta cgaagtattt gccttgccgc aaagtacaga tatctcaact tttggttcac
     4501 tgcttaatat ggtattacca aagtttatta agcatgacgg ggcagttatc aagtatcttg
     4561 ataaacgaag tagtgctgaa gaattagtga aagtcgaagt aatagaacgc tacactgtaa
     4621 gcggaaaagc attgcgcgct ggggcactaa cgc
//
LOCUS       DAOPCD010000054         4637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_15428_length_4637_cov_3.977957, whole genome shotgun sequence.
ACCESSION   DAOPCD010000054 DAOPCD010000000
VERSION     DAOPCD010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15428_length_4637_cov_3.977957"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PLQ55_02165"
     CDS             <1..1153
                     /locus_tag="PLQ55_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPK28772.1"
                     /translation="MWYLHLFTREQPDLNYENPKVLEEVKSILRFWLDKGVAGFRCDV
                     INLISKTSLKDGKKRVYKTGKEHYLNAPGCHLILKELNDEILEPYAAYTVGETMDITI
                     DAAKDYLDGELTQVFAFDQVQVDYFLLPTFKRKYKPHRMVNALKKWQLKIPWNTVFYE
                     NHDLPRSVSRFGSKKYYKESVTALLASVLFLRGTPYIYQGQEIGMTNVDFGGIENIED
                     VSSRNVYNLLRKFKVPRKLAWRFVNNFTRDHGRSPVQWNDKKHAGFTTGKPWLMVNPN
                     YTEINVEAQEIDQDSVLHHYRKMATLRQKTKAFKNGDLEFKKTHRDILAFTRTDDLNE
                     YLVVINLASKARTHKLNLDGEVLYSNYQEFSDKAKKLRPFEVQIIKRLD"
     gene            1220..1699
                     /locus_tag="PLQ55_02170"
     CDS             1220..1699
                     /locus_tag="PLQ55_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HPK28773.1"
                     /translation="MKRKDYINWDEYFMGIAILSSKRSKDPSTQVGACIADDENKVVS
                     LGYNGMPRGIDDDVIPWEKGEDINNKYFYVCHAELNAILNTRSGASLKGCRLYVTLFP
                     CNECAKACIQVGIKEIVYLDDKYHEQVNMRASRHMLKLAGVKLTPFKGKVLDPKFNE"
     gene            1692..2507
                     /locus_tag="PLQ55_02175"
     CDS             1692..2507
                     /locus_tag="PLQ55_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HPK28774.1"
                     /translation="MNKYALEIMNLNYSYDKNVEVLNGVTFNVKAGTYVSVIGHNGSG
                     KSTLAKLIAGILDVGKGSVIKVFGKPLSSYDDERPDIGIVFQNPDNQFIGATVRDDIA
                     FGLENKRVAHAEMDDIINEYAAKVKMSEFLDKEPSNLSGGQKQRVAIAGVLAMKPRIL
                     LLDEATAMLDPRGKREIKEIVTELRKEVPDLTIISITHDLEETLHSDEIIVMNEGKIV
                     LQGTPEDIYKNHETLTSIRLDLPFIFRIKLELEKQGIKVKSKDLESLAEELWP"
     gene            2498..3457
                     /locus_tag="PLQ55_02180"
     CDS             2498..3457
                     /locus_tag="PLQ55_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HPK28775.1"
                     /translation="MAIKFHHIYFSYAPKSPFQYDALHDINLEIKQNSFTAVIGHTGS
                     GKSTLVQHINALLVPTSGAVKVEDFLILPCKKKKYLRQLNRKLKSKKVSAEQKQRLAF
                     LKSIVESGAAYKVKELRKKVGVVFQFPEYQLFEETVIKDVAFGPKNFGVSNEEALALA
                     KNALTLVGIDESFYSRSPFELSGGEKRRVAIAGILALKPEILVLDEPTAGLDPLSAHE
                     MIEVFANIHRAGTTIVLVTHDMDLVLRYASDVVVMKDGKIVEETTPQKLFADVKEEYS
                     LEVPAFYAFMQALVQRGCPLDITKINTVSDLAKMVKNLKEQAK"
     gene            3454..4338
                     /locus_tag="PLQ55_02185"
     CDS             3454..4338
                     /locus_tag="PLQ55_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPK28776.1"
                     /translation="MKNISLGRYMPADSFMHKADPRIKFFGLVVFMVLIFLKFANPAM
                     DFLMYGIYAVVVFILMKVTKLKIFSLFKQLKSLWFMMIFLLIINIFTFRNSEGNILFM
                     IGNFAIKDAALYQTLYIFIRLVLMIALTSVLTASTRPLDLTYAIEWYMSPLKVIRFPV
                     HEIAMTLSIALRFIPTLLDETGRIMRAQESRGVDFKGGKVKEKLRGVVALIVPLFVSA
                     LQRSDELANAMIARGYDPQAKRTRYRILRFTWRDLIVLLLAVSFLTLVILLRVYHIDL
                     ANMLYVNIQNLLSLWGLK"
     gene            4338..>4637
                     /locus_tag="PLQ55_02190"
     CDS             4338..>4637
                     /locus_tag="PLQ55_02190"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HPK28777.1"
                     /translation="MRYVAIVKYLGTNYFGWQRQPFEISVQQVIEDKLSTIFNTEIVI
                     YASGRTDARVHAYGQVFHFDAPPFPVSKLKYALNKMLPGDIEIVKLQKAKDDFHAR"
BASE COUNT         1520 a          732 c          960 g         1425 t
ORIGIN      
        1 catgtggtac ttacatcttt ttacacgaga acaacctgat ttaaattatg aaaacccaaa
       61 agtactagaa gaagttaaaa gtattttacg cttttggctt gataaaggtg tcgcaggttt
      121 ccggtgtgat gttattaatt taattagtaa aacttcactt aaagacggga agaagcgcgt
      181 gtataaaaca ggaaaagagc attacttaaa tgcacctggg tgtcacttaa tcttaaaaga
      241 acttaatgat gaaatactag aaccttacgc ggcttacacg gttggcgaaa caatggatat
      301 tactattgac gctgctaaag attatttaga tggtgaatta acgcaagtgt ttgcctttga
      361 tcaagtgcaa gtcgattact ttttattacc aacatttaaa cggaaatata aaccgcatcg
      421 gatggttaac gccctaaaga aatggcaact taaaatacct tggaatacag tcttttatga
      481 aaatcatgac ttgccacgta gtgtgagtcg ctttggtagt aaaaagtatt ataaggaaag
      541 tgttacggca cttttagcgt cagtcttatt cttacgcgga acaccgtata tttatcaagg
      601 acaagaaatt ggaatgacaa atgttgattt tggcggcatt gaaaatattg aagatgtaag
      661 ttcgcgtaat gtttataact tactccgtaa atttaaagta ccgcgtaaac ttgcgtggcg
      721 ctttgtgaat aactttacgc gcgatcatgg ccgcagtccg gtacagtgga atgataaaaa
      781 gcacgctggt tttactactg gtaaaccgtg gttaatggtg aatccaaact atacagaaat
      841 taatgttgaa gctcaggaaa tagatcaaga ctcagtatta catcattatc gaaaaatggc
      901 gactttacgg caaaaaacaa aagcttttaa aaatggcgac ctagaattta agaaaacaca
      961 tcgtgacatt ttagcgttta cgcgcacaga tgatttaaac gaatatttag tcgtcattaa
     1021 tttagcaagt aaagcacgca ctcataagtt aaatcttgat ggtgaagtac tttattccaa
     1081 ctatcaagag tttagtgata aggctaaaaa gttgcgccca tttgaagtgc aaattataaa
     1141 acgcttagat taagactctt aactagccct taacctatag catttatgct ataattagcg
     1201 ctgttaaggg aggctaatca tgaaacgtaa agattatatt aattgggatg aatattttat
     1261 gggaattgcg attctttctt cgaaaagaag taaagaccca agtacccaag ttggtgcatg
     1321 tattgctgac gatgaaaata aagtcgtcag tttaggttat aacgggatgc ctcgcggaat
     1381 tgatgatgat gtaattccgt gggaaaaagg tgaagatatc aataataaat atttttacgt
     1441 atgtcacgct gaacttaatg ccattttgaa tacaagaagt ggagcctcac ttaaaggatg
     1501 tcgcttgtat gtaacacttt tcccatgtaa cgaatgcgct aaagcttgca ttcaagtcgg
     1561 gattaaagaa atagtgtatc ttgatgacaa gtatcatgaa caagtgaata tgcgcgctag
     1621 tcgccatatg cttaaattag caggagtgaa attaacacca tttaaaggta aagtgttaga
     1681 ccccaaattt aatgaataaa tatgccttag aaataatgaa cttaaattac tcttacgaca
     1741 aaaatgtcga agttttaaac ggcgtcactt ttaatgttaa agcgggcact tatgttagtg
     1801 ttatcggtca taacggcagt gggaaatcaa cattagcgaa actcattgct ggtattttag
     1861 acgtcggtaa agggagtgtc attaaagtat ttggtaagcc tttatcaagt tatgatgatg
     1921 agcgaccaga tatcgggatt gtttttcaaa accccgataa ccaatttatt ggtgcaacgg
     1981 tgcgtgatga tattgctttt ggtctcgaaa ataaaagagt agcccatgcc gagatggatg
     2041 acataattaa tgaatacgcc gctaaagtaa aaatgagtga gttcttagat aaagaaccaa
     2101 gcaacttaag tggtggacaa aagcaacgcg tggcgattgc tggtgtgtta gcgatgaaac
     2161 cacgcatttt actactagat gaagcgaccg cgatgcttga tccacgtggg aaacgcgaga
     2221 ttaaagaaat tgtgacagag ttaagaaaag aagtaccaga cttaacaatc atcagtatta
     2281 cgcatgactt agaagaaaca ttacatagtg atgaaattat tgtgatgaat gaaggcaaaa
     2341 ttgtgctgca aggtacaccg gaagacattt ataagaatca cgaaacatta acgagtattc
     2401 gccttgattt accctttatt ttccggatta aacttgaact tgaaaaacaa ggaattaaag
     2461 ttaagagtaa agatttagaa agtcttgcgg aggaattatg gccataaagt ttcaccatat
     2521 ttattttagt tatgcgccaa agtctccttt tcaatatgat gcattgcatg atattaattt
     2581 agaaattaag caaaatagtt ttacagcggt tattggtcac actggtagcg gaaaatcaac
     2641 gcttgttcaa catattaatg cacttttagt cccaacttct ggtgccgtta aagttgaaga
     2701 ttttcttatt ttgccttgta aaaagaaaaa gtacttacga caattaaacc gtaagcttaa
     2761 aagtaaaaaa gtaagtgccg aacaaaagca aagactagcg tttttaaaaa gtattgttga
     2821 aagtggcgct gcttataaag ttaaagaatt acgaaaaaaa gttggcgttg tctttcagtt
     2881 tcctgaatat caactatttg aagagaccgt cattaaagat gttgcttttg gcccgaaaaa
     2941 ttttggggtg agtaatgaag aagcactagc gttagcgaaa aacgctttaa cattagtcgg
     3001 gattgatgaa tccttttata gtcgttcccc atttgaactt tccggtggtg aaaaacgccg
     3061 ggtggcaatt gctggtattt tagcgcttaa gcctgaaatt ttagtgcttg atgaaccaac
     3121 tgcgggtcta gatcctttaa gcgcccatga aatgatagaa gttttcgcta atattcacag
     3181 agcggggaca acaatcgtgt tagtcacaca tgatatggat ttagtgctgc gttacgctag
     3241 tgatgtggtg gtgatgaaag acgggaagat tgtcgaagaa acaacaccac aaaaactttt
     3301 cgctgatgtg aaagaagaat atagtttaga agtaccagca ttctatgctt ttatgcaagc
     3361 gttagtgcag cgcggctgtc cacttgatat tactaaaata aatactgtaa gtgatttagc
     3421 aaaaatggtg aaaaacctta aggagcaagc aaagtgaaaa atatctcctt agggcgttat
     3481 atgcctgctg attcatttat gcataaagct gatccacgga ttaaattttt tggtttagtc
     3541 gtatttatgg ttttaatctt cctaaagttt gctaatcctg ccatggactt tttgatgtac
     3601 gggatttacg ctgttgttgt ttttatttta atgaaagtaa caaaattaaa gattttttca
     3661 ctctttaaac aactaaaaag cttgtggttt atgatgattt tcttactaat aattaatata
     3721 tttacattta gaaatagtga aggtaatatc ttattcatga ttggtaactt tgcgattaaa
     3781 gatgcagcgc tttatcaaac actatacatt ttcattcgcc ttgtgttaat gattgcttta
     3841 acatcggttt taaccgcaag tacgagaccg ctagacttaa cttatgcgat tgagtggtat
     3901 atgagtccgc ttaaagttat tcgttttcca gttcatgaaa tagcgatgac actgtcaatt
     3961 gcactccggt ttattccgac tttacttgat gaaacgggac gaattatgcg agcgcaagaa
     4021 agtcgtggtg ttgattttaa aggcgggaag gtaaaagaaa aattacgcgg tgttgtcgca
     4081 cttattgttc cgctttttgt tagtgccctc caacgcagcg atgagctcgc taatgcgatg
     4141 attgcgcgcg ggtatgaccc acaggctaaa agaacgcgtt atcggatttt acggtttact
     4201 tggcgtgatt taattgtttt attactagcg gttagttttt taacgcttgt tattttacta
     4261 cgtgtctatc atattgattt agcaaatatg ctctatgtta atatccaaaa tttactatct
     4321 ctttgggggc ttaagtaatg cgctacgtcg caatagtaaa gtacttaggc acaaattact
     4381 ttgggtggca aaggcaaccc tttgaaatta gtgtccaaca agtaatagaa gataagttat
     4441 caacaatttt taatacggaa attgtaattt atgcttcagg tagaacggac gctagagttc
     4501 atgcttacgg gcaagtgttt cattttgacg ctccgccttt tcctgttagt aaattaaagt
     4561 atgccttaaa taaaatgcta cctggtgata ttgaaattgt taaattacaa aaagcaaaag
     4621 atgactttca tgcgcgg
//
LOCUS       DAOPCD010000055         4576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_15661_length_4576_cov_4.012387, whole genome shotgun sequence.
ACCESSION   DAOPCD010000055 DAOPCD010000000
VERSION     DAOPCD010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15661_length_4576_cov_4.012387"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PLQ55_02195"
     CDS             <1..418
                     /locus_tag="PLQ55_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28778.1"
                     /translation="AISAITYPLFSLLLPRKLKDAKLILPRIAISLGLTFIALVIFMA
                     PAANFVPAMSAVKETMSEGTLLYKFLNPEVINFLELFTPQKSVMLKIVDIGNISGKIK
                     IFEEFTVDGVVIQLKDAVEALLEALKTIPYEAPAAG"
     gene            complement(424..807)
                     /locus_tag="PLQ55_02200"
     CDS             complement(424..807)
                     /locus_tag="PLQ55_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HPK28779.1"
                     /translation="MKNVYTNMCMVYSGDKILVLNRVKKDWPGLTFPGGHVEDGELFS
                     ESVIREMKEETGLTIRNPEYVGQIVWPLPEQGINDVALLYRTNEFAGELLESVEGHVF
                     FIKKSELGNYQHSLDFDKVLELMFK"
     gene            899..2107
                     /locus_tag="PLQ55_02205"
     CDS             899..2107
                     /locus_tag="PLQ55_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPK28780.1"
                     /translation="MHINKITVVGLGYVGLAQALLFNEDYNVVGYDTDTKVVASLNRG
                     ILHIKDEDFARALARKKLMATSDITYAFQNSDLVIIATPTHFNEEKQTFDLSSIDETL
                     QKIIASNRSPLVIIKSTVNVGATRYFMEKYPSLKIFFVPEFLREGHALFDNYYPARII
                     VGYDKNDTNHRQIAIQIANLFKKYTKSIDDVPLLITGTLEAEAIKLFANSYLALRVSF
                     FNEIDTFALSKDLEVKDIIEGVGLDSRIGTHYNNPSFGYGGYCLPKDSKQLKAQFNLI
                     PEELISATVNANSVRKEFIANYIADIVKKSNNKDYVVGIYRLTMKKDADNMRFSSIID
                     VVRLLKEQNIKMIIYEPLLNETTYQGITLINNLETFKKESSLIVTNRMHSDLKDVTNK
                     IFTRDVYYRD"
     gene            complement(2118..2414)
                     /locus_tag="PLQ55_02210"
     CDS             complement(2118..2414)
                     /locus_tag="PLQ55_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPK28781.1"
                     /translation="MYCEKLITLRRNRGLTQQQLADALMLSQSMISKIERGITRLDPM
                     LAIRFADFYNVSLDYLFGRSERQLQISEKDIENLSEQDKNEMLAFIIKLINEKK"
     gene            complement(2584..4275)
                     /locus_tag="PLQ55_02215"
     CDS             complement(2584..4275)
                     /locus_tag="PLQ55_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPK28782.1"
                     /translation="MNNKVPLSEYPRMIMVRDSYLSLNGVWEYAIRENDERVPIEFDG
                     DILVPFSPETPLSGVNRTVRPNDVLFYRKRFSLPKGFKKEYVTLHFGAVDQVVTVYLN
                     GTKIGFNQSGFHPFSIEISKVLKIDNELVLVVRDYSDTRFYARGKQKIKRGQIWYTPQ
                     SGIFMPVWLESMPRDYIEKLKITPRYDESLVEITVFTNSKDNDAVIIFDGKEYTVKPN
                     KVYKLKVKDFISWSPDNPHLYPFKVKYCEDEVDSYFAMRKISVDTDEEGIKRIYLNNK
                     ILFNTGLLDQGYYHEGYLTPPDDETVVYEIKVAKEMGFNVLRKHIKIEWERFYYHCDR
                     LGMLVWQDFVNGGEPYNFVTVHVPAVLNIYFKDTNYKRFARQNALGRELFYAEAKTTI
                     EHLYNYPSIVLWTIFNEGWGQFDSHKLLPFVLELDNTRLIDVNSGWHDMGLGQFRSHH
                     VYFRKYRFKKDKLDRCVILSEFGGYAHRIPGHYYGVANFGYRKYNTKEELEKAVINLY
                     EKEIIPAVKQGLAAAIYTQLSDVEDELNGVLTYDRKVVKIDPKVMRELNEKLLKA"
BASE COUNT         1506 a          827 c          715 g         1528 t
ORIGIN      
        1 ggcaatttca gcaattacct atccgttatt tagtttatta cttccacgta agttaaaaga
       61 cgctaaactc atccttccgc gtattgcaat tagccttggc ttaaccttta tcgcccttgt
      121 tatctttatg gctcccgccg caaactttgt accagcgatg agtgctgtca aagaaacaat
      181 gagtgaaggc acattgctct acaaattctt aaatcccgaa gttattaatt tcttagaatt
      241 atttacacca caaaaatcag taatgttaaa aattgttgat attggaaata tttctggcaa
      301 gatcaaaatc tttgaagaat ttactgttga tggggttgtt attcaactaa aagatgccgt
      361 tgaagcctta cttgaagcac ttaaaactat accttacgaa gctccagcag caggataatc
      421 ttattattta aacattagtt ctagaacttt gtcaaaatca agtgagtgtt ggtaattacc
      481 aagctcactt tttttaatga aaaagacgtg accttcaaca ctttctaaaa gttcaccagc
      541 aaattcattt gtgcgatata aaagggcaac atcatttatc ccttgctcag gaagcggcca
      601 gacgatttga ccaacgtatt ccggattacg aattgttaaa cctgtttctt ctttcatttc
      661 acgaattacg ctttcactaa ataattcacc atcttcaaca tggccacctg gaaaagtaag
      721 gcccggccaa tcttttttaa cacggttaag cactaaaatc ttatcacctg aataaaccat
      781 gcacatattt gtgtaaacat ttttcatatt aaaatcgtac cataattcaa aagttttaga
      841 aaaaaggtac tctgcattag tgaataattt gataaatgtt ataatttaag ggaagcatat
      901 gcatattaat aaaattacag ttgttggatt gggatacgtc ggcttagcgc aagccttact
      961 ttttaatgag gactataatg ttgttggtta tgacacagat acgaaagttg tcgctagttt
     1021 gaatcgcggt attttacata ttaaagacga agactttgcg cgagcgcttg ctcgtaaaaa
     1081 actaatggca acaagtgaca taacttatgc attccaaaat agtgatcttg ttattattgc
     1141 gacacctacc cattttaatg aagaaaaaca aacatttgat ctctcatcaa ttgatgaaac
     1201 tttacagaaa attatcgcaa gtaataggag tccgcttgtt attattaaat caacggttaa
     1261 tgttggcgcg actcggtatt ttatggaaaa gtatccatca ttaaaaatct tttttgtccc
     1321 tgaattttta cgcgaaggtc atgccctttt tgataattat tatccagcgc gaattattgt
     1381 cggctacgat aaaaatgaca caaaccaccg gcaaattgct attcaaattg ctaatctctt
     1441 taaaaaatac actaaatcta ttgatgatgt cccgctttta attacgggca ctttagaagc
     1501 tgaagcaatt aaattattcg ctaatagtta tttagcgctc cgcgtatctt tctttaatga
     1561 aattgataca tttgcgttat ctaaagactt agaagttaaa gatattattg aaggtgttgg
     1621 cttagattcg cgcattggga cacattacaa taatccttca tttggttatg gtggttattg
     1681 tttaccgaaa gatagtaaac aactaaaagc acaatttaat ctaattcccg aagaattaat
     1741 cagtgcgact gttaacgcaa atagtgtgcg taaagagttt atcgctaatt atatcgccga
     1801 cattgtaaaa aagagcaata ataaagatta tgtcgttggt atttatcgct taacaatgaa
     1861 aaaagatgct gataatatgc gcttttcatc aatcattgat gtagttagat tactaaaaga
     1921 gcaaaacatt aaaatgatta tttatgaacc gcttttaaac gaaacaactt atcaaggtat
     1981 tacgctcatt aataatttag aaacatttaa aaaagaaagt tcactaattg tcacaaaccg
     2041 tatgcatagt gacttaaaag atgtcactaa caaaatattc acccgcgatg tctattatcg
     2101 cgattaatat atcttcatta tttcttttcg ttaataagtt taataataaa cgctaacatc
     2161 tcatttttat cttgttctga taaattttca atatcttttt cgctaatttg taactgcctt
     2221 tcactacggc caaacaagta atcaagtgaa acattataaa aatctgcaaa acgaattgct
     2281 agcattggat caagccgagt tatgccgcgt tctattttag aaatcattga ttgcgacaac
     2341 attaaagcat cggcaagttg ctgctgggtt aaaccacgat tacgacgtaa agtaattaat
     2401 ttttcgcaat acatgatttt atcttaacta aactaaaacg cttttacttt attaattgct
     2461 taatattcta atttagaata aaatggcttt acaaatattc tgtaatggaa tattaaaaag
     2521 acattatcaa tgcataattg gcaggaaccg aatcgcaaag ataatgtctt ttctttaaat
     2581 gggttaagct tttaagagtt tttcgttaag ttcgcgcata acttttggat caatcttaac
     2641 aacctttcgg tcataagtta atacaccatt taattcgtct tcgacatcac ttaattgggt
     2701 atatatcgcc gctgctaggc cttgcttaac tgcaggaata atttcttttt catacaggtt
     2761 aattaccgct ttttctaatt cttctttagt gttatatttg cgatatccaa agttagcgac
     2821 cccgtaatag tgtccaggta tccgatgcgc ataaccgcca aattcactta ggataacgca
     2881 gcgatcaagc ttatcttttt taaagcgata tttacgaaaa taaacatgat gactgcggaa
     2941 ctgtccaagc cccatatcat gccaacctga attaacatct attagtcgag tattatcaag
     3001 ttcaagcaca aacgggagta atttatgact atcaaattgg ccccagcctt cattaaatat
     3061 tgtccataaa acaatggacg gatagttata taaatgttca atcgttgttt tagcttcagc
     3121 gtagaataat tcgcgtccaa gggcattttg ccgtgcaaaa cgcttataat ttgtatcttt
     3181 aaagtaaata tttaacactg ctggaacatg tacagtaacg aagttatacg gttcgccacc
     3241 attaacaaaa tcttgccaca ctagcatccc taagcgatca caatgataat aaaagcgttc
     3301 ccattcaatt ttgatatgtt tccgtaaaac attaaagccc atctcttttg cgactttgat
     3361 ttcatagact accgtttcat catcaggcgg tgttaaataa ccttcgtgat agtagccttg
     3421 atcgagtaat cccgtattaa aaagtatctt attatttaaa tatatgcgtt taatcccttc
     3481 ttcatctgta tcaacactaa ttttacgcat cgcaaagtac gaatcaactt catcttcaca
     3541 gtatttcact ttaaagggat agagatgcgg gttatcaggt gaccaactaa taaagtcttt
     3601 aaccttaagc ttatatactt tgttaggttt tacagtatat tcttttccat caaaaataat
     3661 tactgcatca ttatcttttg aattagtgaa aacagttatc tccacaagtg actcgtcata
     3721 gcgcggagta atctttaatt tttcaatata atcacgcggc atactttcaa gccaaactgg
     3781 cataaaaata ccgctctgtg gggtatacca tatttgtccg cgtttaatct tttgtttacc
     3841 acgggcataa aacctagtgt cactataatc acggacaacg agcactagtt cattgtctat
     3901 ttttaggact ttactaattt caatagaaaa cggatggaaa ccactttgat taaagccaat
     3961 tttagtacca tttaaataca cagttacaac ttgatcaact gcgccaaaat ggagcgttac
     4021 atattctttt ttaaaaccct ttggtaacga aaagcgttta cgataaaaaa gaacatcatt
     4081 tggtcttact gtgcggttaa caccagaaag cggtgtttct ggtgaaaaag gcactaaaat
     4141 atcaccatca aactcgattg gtactctttc atcattttcc ctaatagcgt attcccatac
     4201 gccgtttagt gataagtaag aatcacgaac cataatcatt cgtgggtatt cacttaatgg
     4261 tactttatta ttcatattca ttgctccttt tcatcttgaa ataaatattt attgttttta
     4321 ttattgcctt aattagtttc taaaggtttg ttcatatatc atctttatta tacaagatta
     4381 gcgccatctt aagaggcaat acataatgtt aactatcatt tattactatg taataagcga
     4441 aattctaaag tatttttgca aaagtaattc atagaatcaa aacatcgtat tgaaatactt
     4501 gcaaaaaagt tggaacttta atatagtata taggctggac cagtagtgta gtggttaaca
     4561 tgtctgcctg tcacgc
//
LOCUS       DAOPCD010000056         4536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_15828_length_4536_cov_3.296809, whole genome shotgun sequence.
ACCESSION   DAOPCD010000056 DAOPCD010000000
VERSION     DAOPCD010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15828_length_4536_cov_3.296809"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..2538)
                     /locus_tag="PLQ55_02220"
     CDS             complement(70..2538)
                     /locus_tag="PLQ55_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPK28783.1"
                     /translation="MKKKKLLIVGLLGLVALSGCKKPSSSVEAPSEVSSEDVLDETVP
                     AVNGVRTFAGENPAAIKDRKDILTLLESYALDTFIGGIPYRDNSAVVLYSSDLKMPAD
                     DYVVGYGFGVGEAEILAPLTGSQVAPERAKYYHTWTSQDPGTINYMDSQESVTGDLHS
                     LISSGYWGTKFNADKTGYEWYPLLANDVERPIPLNFDEATGTATKWRVPLKVGGDLKY
                     NTLGVHSEFAGLEVKLEDYLVPFKLMVETGWFRGTDLASKDSGFVGVAEELQKEVGER
                     SIERVQGIKLNVAEQAMEFEFNSAKSPFYAMYNLASGLFMPIPQSFIDKVGVENYGKA
                     DIDNTLSLGVYNLEIWEKDKQVVFKKNPLWIEADRYTHEGYVFTILDDSNTAFQQFLA
                     NNLHSAGVPASYLKEYRNDERARQTKGNTTWKLQVNAANQERWNELFGEEGSIAPGGE
                     WELKPVMSNLDFLNGVYYSLNRDHLADTTGSRPALGFFSEAYMIDPESGVSWRVSEEG
                     QAVLEGRSTETNGFNKEAAGQLFNKAMNDLVAEGKYTRGTAENPTVIRPRIHFQSVQQ
                     TVDEGGPLKRMIEAAFNDAVEGFKLDIELFATQNWMDAYYAMMFGEFDFALGTISGNT
                     LDPLSFMLTLCSDNRSGFTLSWGHDTGHTYNSYEVGGLVFKGERYSYDALLESTLGLT
                     IIEEGKTVPSFSLKGLDFGFNEADPTKYDFVAQGTYYVDPAGEYVITPDPNGTKEETG
                     GFLALVFYNSEGEFQSIVPLPATTFENGAWTVVAEGISVPEDAHYMDIYISTIHTYKG
                     IPEYRANLYELLWSWFAPEAPAGE"
     gene            complement(2764..3882)
                     /locus_tag="PLQ55_02225"
     CDS             complement(2764..3882)
                     /locus_tag="PLQ55_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28784.1"
                     /translation="MAQKSVTFRTVFKRYKRERRSIKSYLLLTLAFLAFSFVTVYLNS
                     TELALLGAILLVFVIFPLIVVFSEYTALIESGAPAPRNSRAYFDLYKNTYRSGRLRHI
                     FNLRNVVVFLIYFLVGMFIAGLGMTLFIYFFDKPVYDQMFNLMLEFQGTSTPDALNVL
                     VTKIELLLEPYIPSQILAYNLVATLGLIFIIVKGVFNIYLSIFIEHRPTTSFIVLRNT
                     FVGDAETKSSLRRVQTGIVLIVFSLYTLFNVGGYFLLQAINPHGPLFLQAELLGLVVL
                     TIALPFTTRFTFYLYHSVMEKKREQILRFAIVELREIIKNPNLPEQTKTYITQVLKVR
                     EQEYAALTAVEAEQKVVDEKAPASNDDTTNEEIDSDNK"
     gene            complement(3864..4523)
                     /locus_tag="PLQ55_02230"
     CDS             complement(3864..4523)
                     /locus_tag="PLQ55_02230"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HPK28785.1"
                     /translation="MLFAVFGVKEYVILALVVVLVLFALVNIFLFFRKKILQRFALLR
                     YFYPVVRKTAVYYDYYLINKLEIQLVSNESLFIDHMLFGNKYIYVINDYLFKGVLTGN
                     AQDSKWILKDKKGQEIMVDSPLLKNKKLVEKLSLRTSLDHATFIAITLVSKDTKITDL
                     EVNDSSNFVIDTRDFMKLILKIESRDIAPLNPEDLLKRVHEFDELNLRKKRGRKWRKN
                     R"
BASE COUNT         1392 a          851 c          853 g         1440 t
ORIGIN      
        1 ccatgtttag taattatagc aaactaatat tagtttgtat ttttaactga taataattgt
       61 accaattaat tattcaccag caggtgcttc tggtgcaaac catgaccaga gtaattcgta
      121 aaggttagca cgatattctg gtataccttt ataggtgtga atggttgaaa tgtagatatc
      181 catatagtga gcatcttctg gaacgctaat gccttccgcc actacagtcc acgcaccatt
      241 ttcaaatgtt gtagctggga gtggaacgat gctttggaat tcaccctcgc tgttatagaa
      301 gactaaggct aagaagccac cagtttcttc tttagtacca ttcgggtcag gtgtaatgac
      361 atattcacca gctgggtcaa cataatatgt accttgagca acaaagtcat atttggttgg
      421 gtcagcttcg ttgaagccaa agtctaaacc tttgagtgag aatgatggaa cggttttacc
      481 ttcttcaatg atggttaagc caagtgtact ttctaagagt gcgtcatatg aataacgttc
      541 tcctttgaag acgagtccac caacttcgta tgaattgtat gtatgaccag tgtcatgacc
      601 ccatgagagg gtaaagccac tacggttgtc actacaaaga gttaacatga atgataatgg
      661 atctaaggtg ttaccactga tagttcctaa ggcgaagtca aattcaccaa acatcattgc
      721 atagtatgcg tccatccagt tttgggttgc aaataattca atgtctaatt tgaatccttc
      781 gactgcgtcg ttaaaggcag cttcgatcat ccgtttgagt ggtccgcctt cgtcaacggt
      841 ttgttggaca ctttggaagt gaatacgtgg gcgaataacg gttgggtttt cagcggttcc
      901 acgtgtatat ttaccttccg cgactaaatc gttcattgct ttgttgaaga gttggccagc
      961 agcttctttg ttaaagccat ttgtttcagt tgaacggcct tcaaggacgg cttgaccttc
     1021 ttcactaaca cgccatgaca caccactttc tgggtcaatc atgtatgctt cactgaagaa
     1081 gcccaatgct ggacggctac cagtggtatc agcaaggtgg tcacgattta atgaataata
     1141 gacaccattt aagaagtcta agttggacat gactggtttg agttcccatt cgccacctgg
     1201 agcgattgag ccttcttcac caaataattc attccaacgt tcttggttgg cagcgttaac
     1261 ttgaagtttc caagtagtgt tacctttggt ttgacgtgcg cgttcatcat tacggtattc
     1321 ttttaagtaa cttgcaggaa cacccgcgct atgtaagttg tttgctaaga attgttggaa
     1381 agcagtgttg ctatcatcta atattgtaaa gacataacct tcgtgggtat agcggtctgc
     1441 ttcaatccaa agtgggtttt tcttaaagac gacttgtttg tctttttccc agatttcaag
     1501 gttgtaaaca ccaagtgata aggtgttgtc gatatcagcc ttaccatagt tttcaacacc
     1561 gactttgtcg atgaatgatt gtgggatagg catgaataaa cctgatgcta agttgtacat
     1621 cgcatagaat ggtgatttgg ctgaattgaa ttcaaattcc atagcttgtt cggcaacatt
     1681 aagtttaata ccttgtacgc gttcaatgct acgttcgcca acttcttttt gtaattcttc
     1741 agcgacacca acgaatccgc tgtcttttga cgctaagtca gttccgcgga accatccggt
     1801 ttcaaccatg agtttgaatg ggactaagta gtcttctaat ttgacttcta agccagcaaa
     1861 ttcactgtgg acacctaaag tgttatattt taagtctcca ccaactttaa gaggaactct
     1921 ccacttggta gcagtaccag tggcttcgtc aaagtttaaa ggaattggac gttcgacgtc
     1981 atttgcgagt aatggatacc attcatatcc tgtcttatcc gcattaaatt ttgtacccca
     2041 atatcctgaa gagataagag agtggaggtc accagtaacg gattcttgtg agtccatata
     2101 gttaatggta cctggatctt gactagtcca agtatggtag tactttgcac gttctggagc
     2161 aacttgtgat cctgtaagtg gtgcaagaat ttcggcttca ccaaccccga atccataacc
     2221 aacgacatag tcgtctgctg gcattttgag gtcgcttgag taaagcacga cagcactgtt
     2281 gtcacgataa ggaataccac cgatgaatgt atcaagggcg taactttcaa gaagtgttaa
     2341 aatatcctta cgatctttga tagcagcagg attttcccca gcgaatgttc taacgccatt
     2401 aacggctgga acagtttcgt ctaagacgtc ttcgctgcta acttctgatg gtgcttctac
     2461 agatgagctt ggctttttac aaccgcttaa tgcgactaat ccgagaagtc caacgattaa
     2521 cagttttttc tttttcataa aactttctcc tttcttatag ttttataggt ttattataca
     2581 tagtacattt agattgccaa gaaataggat gaaaatattt tcatgataac gcttacactt
     2641 gttccaaatt tgaaaaaata agtgtttttt accaaattta gtgaatattt ataaaataaa
     2701 agccccgttt aaatagggac aattaatact aattgttgga taaatcgatg aagtcaacag
     2761 tttttattta ttatctgaat cgatttcttc attagtggtg tcgtcattac tagcaggcgc
     2821 tttttcatca acgacttttt gttctgcttc cacggcagta agtgcagcgt attcttgttc
     2881 acgaactttt aaaacttgtg taatgtaagt ttttgtttgt tcgggaagat ttggattttt
     2941 aataatttca cgtaattcaa caatggcaaa acgaagaatt tgttcgcgtt ttttctccat
     3001 aactgaatga tataaataaa aggtaaaacg cgtagtaaat ggtaaagcga tagttaacac
     3061 aactaaacca agtaattcag cttgtaagaa aagcggacca tgcggattaa tggcttgtag
     3121 taagaaatat ccgccaacat taaataaagt atatagcgaa aagacaatta aaacaatccc
     3181 tgtttgaaca cggcgtaaac tacttttagt ttcagcgtca cctacaaaag tattgcgaag
     3241 cacaataaag ctcgttgttg gacgatgttc aataaatatc gaaaggtaaa tattaaaaac
     3301 accttttact atgataaaga taaggccgag tgttgccaca aggttatatg caagtatttg
     3361 acttggtata taaggttcga gcaataattc tatttttgtt actaaaacat ttaatgcgtc
     3421 tggtgtagat gttccttgaa actctagcat taagttaaac atttgatcgt acacaggctt
     3481 atcaaagaaa taaatgaaga gtgtcattcc taatccagct ataaacattc cgaccaaaaa
     3541 gtaaataagg aagacgacaa catttcgtaa attaaatata tggcgtaatc ttccgctgcg
     3601 gtaagtgttt ttatacaaat caaaatacgc tcgactatta cgtggcgctg gtgcaccaga
     3661 ctcaataagt gctgtgtatt ctgaaaatac aacaattagt ggaaagataa cgaacactag
     3721 caaaatcgct ccaagtagcg ctaattcggt ggaatttaaa tatactgtta caaagctaaa
     3781 tgctaaaaag gctaacgtta ataataaata gcttttaata gaacgccgtt cgcgtttata
     3841 gcgtttaaat actgtacgaa atgttaccga tttttgcgcc attttcgtcc tctttttttg
     3901 cgtagattta gttcatcaaa ttcatgaacc cgcttcaaca aatcttctgg attaagcggg
     3961 gcaatatctc tactctcaat ttttaaaata agtttcataa aatcacgagt atctatgaca
     4021 aagtttgaac tatcattaac ttccaaatca gtgattttcg tatctttact cacgagtgta
     4081 atagcaataa aagtggcatg atcaagcgat gttctaaggc ttagtttttc cactaatttt
     4141 ttatttttta aaagcggact atcaaccata atttcttgtc cctttttatc ttttaaaatc
     4201 cacttggaat cttgagcgtt tccagttaaa acacccttaa ataagtaatc attaatgaca
     4261 taaatatact tattaccaaa aagcatgtga tcaataaata aagattcatt tgaaacaagt
     4321 tgaatctcta gtttattaat caaataataa tcgtaataca ccgctgtttt acgcactaca
     4381 ggataaaaat atcgcaataa tgcaaagcgt tgtaaaatct tttttctgaa gaaaagaaaa
     4441 atattgacta gtgcaaatag cactagcaca accactagcg ctaatatcac atattcctta
     4501 acgccaaaaa cagcaaataa cataattatt cttcag
//
LOCUS       DAOPCD010000057         4456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_16145_length_4456_cov_3.188139, whole genome shotgun sequence.
ACCESSION   DAOPCD010000057 DAOPCD010000000
VERSION     DAOPCD010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16145_length_4456_cov_3.188139"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2729
                     /locus_tag="PLQ55_02235"
     CDS             <1..2729
                     /locus_tag="PLQ55_02235"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPK28786.1"
                     /translation="KCIKKEDISRITFTALPFVLAFFIPFILAIILFSKHGYYPFSAD
                     GHSLAMVDLRGQYIAFFRYYKTILDGDNTILYTLGKVTGGDFLSIFTYYLASPFNLVL
                     KFVANVELPKALMWLVIIKIALSGLTAYISLAAINKNGFVNLIFSIAYALMAYNFVYY
                     SNIMWLDGVLALPLVALGIHLLTQEKSALLYIFALAYVIATSWYIGIMVAMFSVFFFL
                     YNFISLNSRMYFRKKLLLTFTLSSLLAGLISFAFWGSALVNILGTKGSMSFTNLSKRI
                     KEFYALVDIERGFLYGNHIGMKDIMEPTSVIYAGALPLFLMIMFFANRAFSRKQRIAA
                     LTLVLIYVVALFNKGVDHLFHGGPAPNWFPCRYGFIFSFIVIYFASLNFREYKATKWY
                     AFIAPVIVYLFLIISLTKNEFAYDKKSILYFSITFCVLVIFFVLNEFIFKQKGEEDNS
                     KRVQKYVTASVSFLLIIVSAINVFGNSNRILSVFSDTVYHESMTAYRNDERIGRAIDF
                     LKKSDNSLYRLEKSFIRSGTYNKANNDSMYFNYNGISHYSSSEKKQVMDYMLKIGFHY
                     NGFNLNYANGSTLAINSYLGVKYLLDNGVNRNFDFVRELPLVDDGLNDDINTYLNTYA
                     LPFLMPIEDTNISYVGDGVYVDGGSSVYWFDMFEYQNHLFKTITRTVKDELGKPKDIF
                     KKATYTKTLSNVTPLPRDYYYKVVSSGTIDYDITLDKNTNYYYYIHGPKSNDLALYDR
                     NIYTTYFSYHGYQINGIRRNTASTRLSVAITKPQESIKLQEAIYYEDLSVLNEYINAI
                     KAIAEVDIKQEKTSKYSASVVTKKDNQYMLLTLPYNQNLRVFIDGKKVETTTRFNIFT
                     GFTIPKAGAHKITIKYVELTYQLGIPVGILSAAGTIVISVLNKKKKEEQEKA"
     gene            complement(2726..4021)
                     /locus_tag="PLQ55_02240"
     CDS             complement(2726..4021)
                     /locus_tag="PLQ55_02240"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HPK28787.1"
                     /translation="MNTFKTKWKEGITKIDSFFNMLFDYFMKFFRKYYLWIAFAFILA
                     ISFFVRYAFFDYLSGDMHNFLLQWFNHLKANGGLKAMGDYPWTNTLGVRAGDYPVSYI
                     TLLALLSYLPFEGIVSIKLLSVFFDYVLAIGAVLIIREFNKNSYFSLLAFAILVFFPT
                     SILNSAVWGQCDQLYVALIVWTVLFLIKKKHFLAMIFLGLALATKLQTTFFLPVLIFM
                     WLAKKFKLRYLLVMFLVIFLTFIPSYIAGAPFSMPFNMYRSQIGLYTNANYGAGSLYA
                     FFEFPKLYAGIKRVGVYVAFIAIGVTLLVLFHYKVPATPKNIIYVTALFSLVSPFFLP
                     HMHERYFYMADAFLIIYVLVFKRKYIYAALMSFSSVLTYTHFLTGQYIFKFLDKDCVR
                     LSALINLGLIIALIIDAKSVLEKEPAEELLELETEQSEV"
     gene            complement(4084..4248)
                     /locus_tag="PLQ55_02245"
     CDS             complement(4084..4248)
                     /locus_tag="PLQ55_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28788.1"
                     /translation="MTNTNIKLITEKTKHELFILKIDVILVRHGLKRTQIVYDRAGQV
                     AVSFVPPKRK"
BASE COUNT         1496 a          705 c          769 g         1486 t
ORIGIN      
        1 ttaagtgtat taaaaaagaa gatatcagtc gtataacatt tactgcttta ccttttgtct
       61 tagctttttt tattccgttt atccttgcta ttattttatt ttcaaaacat ggatattacc
      121 cttttagtgc cgacgggcat tctttagcga tggttgattt gcgcggacag tatattgcct
      181 ttttccgtta ttataaaacg atattagatg gcgacaatac tattttatat acattaggga
      241 aagtaactgg tggtgatttt ctttcaattt tcacttacta cttagcatcc ccctttaatt
      301 tagtgctaaa gtttgttgca aatgttgaat taccaaaagc cttaatgtgg cttgtcatta
      361 ttaaaatagc actaagtggt ttaactgctt atataagctt agcggctatt aataaaaacg
      421 gttttgttaa cttaatcttt agtattgcgt atgcactaat ggcgtataac tttgtctatt
      481 attcaaacat tatgtggctt gatggagtat tagctttacc gcttgttgct ttaggtattc
      541 atctacttac acaagagaag agtgcgcttt tatacatttt tgcgttagcc tatgtcattg
      601 ctacttcgtg gtatatcggg attatggtgg caatgttttc tgttttcttt ttcctttaca
      661 attttataag tctaaattca cgaatgtatt ttcggaaaaa attactttta acctttactt
      721 taagttcact actagcaggt ttaatttcgt ttgccttttg gggttcagcg cttgtaaata
      781 ttttagggac aaaaggctca atgagtttta ctaacttatc aaaaagaatt aaagaatttt
      841 atgcgcttgt tgatattgaa cgcggtttcc tatacgggaa tcatattgga atgaaagata
      901 ttatggaacc cacaagtgta atttacgctg gtgcattacc actattttta atgattatgt
      961 tctttgctaa tcgtgcattt agtcgtaaac aaagaattgc agctttaact ctagtgttaa
     1021 tttatgtagt agcgttattt aataaaggtg ttgatcatct cttccacggt ggtccagccc
     1081 cgaactggtt tccgtgccgc tacgggttta tttttagttt tattgttatt tactttgctt
     1141 cccttaactt tagagagtat aaagcgacaa aatggtatgc ttttattgct ccggtaatcg
     1201 tttatttatt cttaattatt tcgcttacca aaaatgaatt tgcgtatgat aaaaaaagta
     1261 ttttatattt tagtataacc ttttgcgtct tagttatttt cttcgtgctc aatgaattta
     1321 tctttaagca aaaaggggaa gaagacaatt ctaaaagagt acaaaaatat gttacggcga
     1381 gtgtttcttt cctgcttata atcgtgagcg cgattaatgt atttggcaat tctaaccgca
     1441 ttttgtcagt gtttagtgat acagtatacc acgaatcaat gacggcatat cgtaatgatg
     1501 aacgcattgg cagagcaatt gatttcctta aaaaaagcga taattcactc tatcgtttag
     1561 agaaatcatt catccgcagc ggtacatata ataaggctaa taatgactca atgtatttta
     1621 attacaacgg tatttcacac tattcttcaa gcgaaaaaaa gcaagtaatg gattatatgc
     1681 taaagattgg atttcattat aacggcttta atttaaatta tgcgaacggt tcaactttag
     1741 cgattaatag ctatttaggt gtaaagtatc tacttgataa tggagtgaat cgtaactttg
     1801 actttgtgcg tgaattaccg cttgtggatg acggacttaa tgatgatata aatacatatc
     1861 ttaacactta cgccttacca tttttaatgc cgattgaaga tacgaatatt agttatgttg
     1921 gagatggtgt atatgttgat ggtggtagca gtgtttattg gtttgacatg tttgaatatc
     1981 aaaatcattt atttaaaaca attacacgca ctgtgaaaga tgaactaggt aaacctaaag
     2041 acatctttaa aaaagcaact tatacaaaga cgctaagtaa tgttactccg ctcccaagag
     2101 attattacta taaagtagtt agttccggca cgattgatta tgatattaca cttgataaaa
     2161 acacaaacta ttattactat attcacggac caaagagtaa tgacttagcg ttatatgatc
     2221 gcaatatata cacgacatat ttttcttatc acggttatca aattaacggt attaggcgta
     2281 acaccgctag tacacgatta agtgtggcaa ttactaaacc acaagaaagt attaaattac
     2341 aggaagcgat atattacgaa gacttaagtg ttttaaatga atacattaat gcgattaagg
     2401 ctattgctga agttgatatt aagcaagaaa aaacatctaa gtacagcgct agtgtagtga
     2461 cgaaaaaaga taatcaatat atgctattaa ctttgcctta taatcaaaac ttacgcgtgt
     2521 ttattgacgg taaaaaagtt gagacaacaa cgcgttttaa tatttttaca ggctttacta
     2581 tccctaaagc cggtgcccat aaaatcacta taaaatatgt tgaattaact tatcaacttg
     2641 gtattcctgt tggtatatta agtgcagcgg gcactatcgt aatcagtgtt ttaaataaaa
     2701 agaaaaaaga agaacaagaa aaagcctaaa cttcactttg ttctgtttca agttcaagta
     2761 attcttctgc cggttctttt tctaatacac ttttagcgtc aattattaaa gcaataatta
     2821 atcctaaatt tattagcgca gaaagtctta cacagtcttt atctaaaaac ttgaagatgt
     2881 attgaccagt taaaaagtgt gtataagtta agacgcttga aaaagacatc agtgccgcat
     2941 aaatgtactt acgtttaaat actaaaacat aaataattaa aaaagcgtcc gccatataga
     3001 aatagcgttc atgcatatgt ggtaagaaaa agggtgacac taatgaaaat agtgcagtga
     3061 catatattat atttttaggc gttgctggta ctttatagtg gaagagcact aaaagtgtaa
     3121 cgccaattgc gataaaagca acataaacac caacacgctt tattccggcg taaagttttg
     3181 gaaattcaaa gaaagcatat aatgatcctg ctccataatt tgcgttcgta tataacccaa
     3241 tttgtgaacg atacatatta aaaggcatac taaaaggagc gccggcaata tagctaggga
     3301 taaaggtaag aaaaataacg aggaacatta ctaaaagata acggagttta aactttttag
     3361 cgagccacat aaagattaac accggcaaaa agaatgttgt ttgtagtttt gtcgctaacg
     3421 ctagtcctaa aaaaatcatt gctaaaaagt gtttcttttt aataaggaat aatactgtcc
     3481 agacgataag cgcaacatag agttgatcac attgacccca cacagcggaa ttaagaatgc
     3541 tcgtggggaa aaatactaaa atggcaaacg ctaatagcga gaaataagag tttttattaa
     3601 attcacgaat tataagtacc gctccaatag cgagtacata atcaaaaaag acactaagta
     3661 atttaattga aacaatccct tcaaatggta aatacgaaag aagggcgagc agtgttatgt
     3721 aagatacagg atagtcccct gctctaacac caagtgtgtt agtccacggg taatcaccca
     3781 ttgcctttaa ccccccatta gcctttaagt gattaaacca ctgtaagaga aagttatgca
     3841 tatcgccact taaataatca aaaaatgcat aacggacgaa aaaggaaata gctaaaataa
     3901 aggcaaaagc aatccataag taatatttgc ggaagaactt cataaaataa tcaaaaagca
     3961 tattaaaaaa tgagtcaatt ttcgtaatac cttctttcca tttagtttta aaagtattca
     4021 tttgcatcct cttacttaag taaagtttac catatattaa aatattactt attgcgtgga
     4081 atactatttt cttttaggcg gcacgaagct caccgctact tgacctgctc ggtcgtaaac
     4141 tatttgcgtt ctttttaaac cgtgtcttac aagtatgaca tcaattttta aaataaataa
     4201 ttcgtgtttt gttttttcgg tgataagttt aatgttagtg ttagtcatag ctaaaacccc
     4261 tttccgtgtc gcggttatta atgaggggca ttaatattag tgttaatgtt actaaccact
     4321 gtatatgttt tattgtatct cataacttta aaaatgacta tgatgaaata taaaaaaagc
     4381 cctacgaagt aggactaatt tttgataact ggtgcagcca gtgagacttg aactcacacg
     4441 ggattaccat acgccc
//
LOCUS       DAOPCD010000058         4396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_16410_length_4396_cov_4.186363, whole genome shotgun sequence.
ACCESSION   DAOPCD010000058 DAOPCD010000000
VERSION     DAOPCD010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16410_length_4396_cov_4.186363"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PLQ55_02250"
     CDS             <1..981
                     /locus_tag="PLQ55_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK28789.1"
                     /translation="AAPLILVGLSVAFAFKTGLFNIGASGQLMMGSFLAVFVGIKTES
                     WILALISGVLGGALWALIPALLKAYRNVHEVVATIMMNYIAMYSSKMLIEWLIFMPIQ
                     QQARPVPTGGQLPTLGLDKLFNFPRLNSGIVIAIITTIIIFVLLEKTTFGYQLKAVGF
                     NREAARYAGINDKRNIVLSLVIAGALAGLAGAVIYLVPAQGERLATKHVISNYGFQGI
                     AVALLGMSHPIGTMGAGLFFGYIEAANLPLQRVGFKREIIDIISASIIYFSAFTLFFQ
                     IYARRFINKMRDFFAKIKKKPQLATAEGVEIETLPPNEEGKEGGQEDDRK"
     gene            1097..2080
                     /locus_tag="PLQ55_02255"
     CDS             1097..2080
                     /locus_tag="PLQ55_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK28790.1"
                     /translation="MYDLLTQTIYSAIPLAIVAMGGMYSERSGVVNIALEGTMMFGAF
                     VGILCLSHIQHAGIDGQHIIFLALLIGAISGVIFSALHAFASITMKSDQTISGTALNM
                     LAAGLAIFMGRALSLGRTEEITFSSTHHRISAIPLLRHIPFFGKILFEDIYLISLFGI
                     LLFIVMGVIAYKTKFGLRLRACGENPHAADAAGINVIRTRYIAVLISGMLGGIGGVLL
                     IIPTTVSFIANVYGFGFLALAVLISGQWKPSRIMIFAIFFGLLRSIAFGFAFIKADLS
                     HIAFLKTFLDKLPTDILNMLPYVVTLIVLAFTSLKSRGPKAAGEPYDQGKR"
     gene            2160..2236
                     /locus_tag="PLQ55_02260"
     tRNA            2160..2236
                     /locus_tag="PLQ55_02260"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2194..2196,aa:Arg,seq:acg)
     gene            2427..3362
                     /locus_tag="PLQ55_02265"
     CDS             2427..3362
                     /locus_tag="PLQ55_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008753856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPK28791.1"
                     /translation="MSKINEIQVLLSKKAELNARLSLLPYDGTPEIKTISGKDYLYVR
                     KRVLNKVTSTYVDVYSDKLYNNLLRNNEEAKKLRREIRKIDKALASFGYKEGILLPNV
                     ILARDFARKNRNAIIYDQVILEGIGTTFPETEAILEQGIIKNARAEDVQKILNLKRAW
                     EFILDDDVLLTKTDFHLISYIAKIVNEGLFIFGGEVRGVSVKIGGSTYYPPIPLESKV
                     KEDINALLSSSLNDVDTAIELCLYIMKTQIFNDGNKRTAVIATNHYLVSKGIGFLAIA
                     YDKVPAFREMLVNYYENKNINEIKNFLKSCIHLVD"
     gene            3531..4004
                     /locus_tag="PLQ55_02270"
     CDS             3531..4004
                     /locus_tag="PLQ55_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPK28792.1"
                     /translation="MTKEMFSEKYLTYKVMVFQISYAYLRNIEDAEEATLDSFMKFYK
                     STKKFQSVNHEKNYLIRVTINTAKDYLKKIKTLPLNEEVHGVYDDNGELDKHYVWAVV
                     DELEGKYKDVIYLRYTADLPYKDIASILKISEALARKYHEIAIKTLRELIGGNYA"
     gene            3997..>4396
                     /locus_tag="PLQ55_02275"
     CDS             3997..>4396
                     /locus_tag="PLQ55_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28793.1"
                     /translation="MPELEKKLHETKTLIPEVPQRLDIKMEERIDGATKVKRPLNLRF
                     RFVLATAIVACVALSATTIALFSHTQKEQQQLFHKLPQSKVLMNLDDDLVNRTAIKAV
                     EKLSPFLTDEKAEDTVLSPATYSLAMAGLSA"
BASE COUNT         1404 a          716 c          883 g         1393 t
ORIGIN      
        1 gctgcaccat taattttagt ggggctttca gtcgcatttg cctttaagac aggcttattt
       61 aatattggcg ctagtggaca attaatgatg gggtcgtttt tagctgtttt tgtggggatt
      121 aaaaccgaat catggatttt agcgcttatt agcggtgtgc tcggtggcgc attatgggca
      181 ttaatcccag ccttacttaa agcttatcgc aatgtccatg aagttgtggc gaccattatg
      241 atgaactata ttgcaatgta ctcttcaaag atgttaattg aatggttaat ttttatgcca
      301 attcagcaac aagcacgtcc tgtgcccaca ggcggacaat taccaacatt aggacttgat
      361 aaattattta actttccgcg ccttaatagc gggatagtaa tagcgataat tacgacaatt
      421 atcatttttg tgttacttga aaaaacaacc tttggttatc agcttaaagc agttggcttt
      481 aaccgtgaag ccgcgcgtta tgcagggatt aatgataaac gtaatatcgt gctctcactt
      541 gtaattgctg gcgctcttgc tggtttagcg ggggctgtta tttacttagt gccagcgcaa
      601 ggcgaaagac ttgccacaaa acacgttatc tcaaattacg gcttccaagg gattgccgtt
      661 gcactccttg ggatgagcca tccaatagga acgatgggag cggggctctt ctttggttat
      721 attgaagcgg cgaacttacc actccaaaga gttgggttta aacgcgaaat tattgatatt
      781 atttcggcaa gtattattta ctttagtgcc tttacgttat tcttccaaat ttacgcacga
      841 cgctttatta ataaaatgcg tgatttcttc gctaagataa agaaaaaacc acaattagca
      901 actgccgaag gagtagaaat tgaaacgcta ccaccgaatg aagaaggtaa agaaggagga
      961 caagaagatg accgaaaata aatcaaagtt caaagttttt cttgctaaac ttccgaaact
     1021 ttcgaaaaaa caaattatta caatcgcagt tttacttgtc ctttatgtgt tacttgctat
     1081 ctttacgagt gataatatgt acgatctttt aacacagacg atttactcgg caatcccctt
     1141 agcaattgtg gcgatgggcg gcatgtatag tgagcgttca ggcgtcgtta atattgccct
     1201 tgaagggaca atgatgtttg gggcatttgt cggaatatta tgcttaagtc atatccaaca
     1261 tgcgggaatt gatggccaac atattatttt ccttgcgctc ttaattgggg ctatttctgg
     1321 cgttatattc tccgccttac acgcttttgc ctcaattaca atgaaaagtg atcaaacaat
     1381 ttcggggaca gcacttaata tgctcgctgc tgggttagca atctttatgg gccgcgcact
     1441 ttcactaggc cgaacagaag aaattacctt ttcaagtacg catcatcgta ttagtgcgat
     1501 tccgctttta cgccatattc cgttctttgg taaaatcctg tttgaagata tttacttaat
     1561 ttcattattc ggtattttgc tctttattgt gatgggtgtt attgcctata agacgaagtt
     1621 tggattacgc ttacgagcat gtggcgaaaa tccgcatgct gcagatgcag ccggaattaa
     1681 tgtcatcaga actcgttata ttgctgtttt aatcagcgga atgctcggcg gtattggcgg
     1741 tgtcttactt attatcccga cgaccgtgag ttttatcgcg aatgtgtatg gatttgggtt
     1801 cttagcactt gccgttctta tcagtggtca atggaaacca agtcggatta tgatttttgc
     1861 gattttcttt ggactcttac ggagtattgc ttttggtttt gcttttatta aggcagactt
     1921 aagtcatatt gcgttcctta aaacattctt agataagtta ccgacggata ttttaaatat
     1981 gttgccatat gttgtaacac ttattgtgct tgcctttaca tcacttaaat cacgcggacc
     2041 aaaagccgca ggtgagcctt atgaccaagg gaaacgctga ttatttgcta aatattacat
     2101 atttaacttt ataacacttg caaaaatcac ttaaataact ataatgttta agtgacgctg
     2161 cggtcatagc tcaactggat agagtgccag actacgaatc tggaggttgg gagttcgatt
     2221 cttcctgacc gcgccattgc gaataaacat gctccctaaa attgcactag agtctagtac
     2281 aatgaaaagg ggagcttttt atttatgaaa tctaattatg tctaatacat gcgataaacg
     2341 aataaatttg acaaggtaaa atttagattt gttttaggga acaaaaggtg tataattaag
     2401 ttagaaagtt ccctaagaga attgttatga gtaaaataaa tgaaatacaa gtattattat
     2461 caaaaaaagc tgaactaaac gcacggctat cgttattgcc gtatgatggc actcctgaaa
     2521 taaaaacaat cagcggcaaa gattatctct atgtgcgaaa aagagttcta aataaagtga
     2581 cttcaaccta tgttgatgtt tatagtgaca agctttataa caatctactt cgtaataatg
     2641 aagaagcaaa aaaacttagg cgtgaaatac gcaaaatcga taaagcttta gcgagttttg
     2701 gttacaaaga aggaatacta ttacctaatg tcattttagc gcgggatttt gcgcgaaaaa
     2761 atcgtaatgc gattatttat gatcaagtta tattagaggg aattggtaca acttttcccg
     2821 agacagaagc aattctcgaa caaggtatta tcaagaatgc tagagcggaa gatgtgcaaa
     2881 aaattcttaa tttgaaaaga gcgtgggaat ttattttaga tgacgatgtt ttattaacga
     2941 aaacagattt tcatcttatt agttatatcg ctaaaattgt taatgaagga ttatttattt
     3001 ttggtggcga agttagaggg gtatctgtta agattggtgg ttcaacttat tatcctccta
     3061 tcccacttga atctaaagta aaagaagata tcaacgcttt attgtcttca tcgttaaatg
     3121 atgtcgatac cgctattgaa ctttgtttat acattatgaa aacgcaaatc tttaatgatg
     3181 gcaataaaag aactgcggtt attgccacta accattattt agtgagtaaa ggcattgggt
     3241 ttttagcaat agcttacgat aaagttcccg cttttagaga aatgttagta aattattatg
     3301 aaaataaaaa tattaatgaa attaaaaact ttcttaaaag ttgtatacat ttagttgact
     3361 agtgcaaact tagcaatata aagggttttt taacaatatt tactattttt tcgcaaaaca
     3421 gtgtttttaa tcgtattata agtagtaggt attaataata atggaaacaa ataaagtgtt
     3481 gatgaattca caaaagtgtc gcgttaatcg taataatagt gtaggtgctt atgactaaag
     3541 agatgtttag tgaaaagtat ctaacctaca aagtcatggt gtttcaaata agctatgcat
     3601 atttacggaa tattgaagat gctgaagagg cgacgcttga ctcatttatg aaattttata
     3661 aaagcacgaa gaaatttcag tcagttaacc atgaaaagaa ttatttaatt agagtaacga
     3721 ttaatactgc aaaagattac ttaaagaaaa ttaaaacgtt acccttaaat gaagaagttc
     3781 atggtgtgta tgacgataat ggtgaacttg ataagcatta tgtatgggca gtagtcgacg
     3841 agttagaagg taaatataaa gatgttattt atttgcgtta tactgcagac ttaccgtata
     3901 aagatatagc aagcattctt aaaataagtg aagcactagc gaggaaatat catgaaattg
     3961 ccattaaaac attgcgtgaa ttaatcggag gaaattatgc ctgaattaga aaagaaatta
     4021 catgaaacaa aaacacttat tcctgaagtg ccacaaagat tagatattaa aatggaagaa
     4081 aggattgatg gtgctacaaa agtaaagcgt ccgcttaact tgcgttttcg ctttgtccta
     4141 gcgacagcca ttgtcgcgtg tgtggcttta agcgcgacga caattgcctt atttagtcat
     4201 actcaaaaag aacaacaaca attatttcac aaattaccac agtctaaagt gctgatgaat
     4261 ttagatgatg acttagtaaa tagaacagca attaaggcgg ttgagaaatt atcccccttt
     4321 cttactgatg aaaaagcaga agatactgtg ctatcaccag caacttactc cttagcgatg
     4381 gctgggcttt ccgctg
//
LOCUS       DAOPCD010000059         4372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_16515_length_4372_cov_4.351633, whole genome shotgun sequence.
ACCESSION   DAOPCD010000059 DAOPCD010000000
VERSION     DAOPCD010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16515_length_4372_cov_4.351633"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1435
                     /locus_tag="PLQ55_02280"
     CDS             152..1435
                     /locus_tag="PLQ55_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPK28794.1"
                     /translation="MKRKTYLRNQTIYQIYARNHTAEGTFVALIKDLPRIKELGVDIL
                     YLLPIHPIGEIARKGELGSPYAIRDYYLINPEHGTLEDFQTFITLAHSYGFKVMIDIV
                     LHHTSPDAELLAEHPEFYYYKNGKLGNKVGDWSDIIDLDYNNRDLWDYMVEMLKYWAS
                     LGVDGYRADVAPLVPLAFWEYARQELDEDYPELIWLSESVEPHFITNLTAAGHVAHHD
                     KEMYSAFDILYDYDVYNDLKAYLEGKAPLKPYLDGVRYQEQNYPKGYLKIRTIENHDT
                     VRIASLCKTDQILRNVTAWSFFQNGVGFIYGGQEVKAPHRPTLFDIDKIDLNVKDQDY
                     YDFMILLVEMKKHPVFAEISSFNIIGDGADDVIVSTLESRVGAAHGFFNLGLKEKEVK
                     TELKDGSYTNVLTNTDIVVNDGKIKVSEPIIIIEE"
     gene            1465..1549
                     /locus_tag="PLQ55_02285"
     tRNA            1465..1549
                     /locus_tag="PLQ55_02285"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1499..1501,aa:Leu,seq:cag)
     gene            1599..2204
                     /locus_tag="PLQ55_02290"
     CDS             1599..2204
                     /locus_tag="PLQ55_02290"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPK28795.1"
                     /translation="MHKVSIRSKLTPHQVFTIPNILSFFRLLLIPLIVFLYVGKELYG
                     WTLVVLIISLVTDIIDGLVARKLNMVTDFGKFIDPVADKLTQLAVLFCLVTRFPLMWI
                     PISIMLLKEILQFALRFVVFRYTGEVHSAEWHGKVATFLIYVMMSLHVIWYGITDVAS
                     TLTITITSLSMLLSCVLYTISSAVIIINIKRRKKDKEQLNN"
     gene            complement(2253..3029)
                     /locus_tag="PLQ55_02295"
     CDS             complement(2253..3029)
                     /locus_tag="PLQ55_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPK28796.1"
                     /translation="MEEYTLLTNKLFSSLKNKEFNLQFQPQINCNNGQINGVESLLRW
                     TTDEGQRVGPNVFIPMLEQTGLIHEVGLWVIDQSLKQHKALVKKGFAPLRFSVNLSVA
                     QFRRDDFVDNVLKLLKKHKVEPQYLELEITESLLSKNFADTIEKLTRLNDAGLHIAID
                     DFGKGYSSLHRLQLVPFNRIKIDKSVVDNITIEPKKVVIAKTIINLAKSLNANITTEG
                     VETKEQAAFMKDLGTDEIQGYYYYSRPLPPKDLEDFLKQK"
     gene            complement(3110..>4372)
                     /locus_tag="PLQ55_02300"
     CDS             complement(3110..>4372)
                     /locus_tag="PLQ55_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPK28797.1"
                     /translation="GMTFFIVKDAHMGASTVWAVYIVFLLYSIVLDSKRDTLFFLIFT
                     VFTQIVLWITFPNVTIVLDNAQYAKRLFIIILSYVAVRYVTSEYDSKFQGYQRFSRGQ
                     EILEKISTNFIAVNQDNVRAKIDEMFMMAAEILQYDQAYLFRFDDKGERATILNAYVR
                     DSEDNSLLLQLGRSFKISEFPQVEPLAGKHSPIFCDDIANLGTHTQDESANFFLSRGI
                     NSFYALPIFVEEKLSGFFVVEYKEKNELTVVESRLHYLKIIANVLGDARKKIIYEKQL
                     YDFAYIDKSTNLPNRNMLINNLEELLLNRDDADKLVVFNVELDNLRMINDTFGHATGE
                     QVVIKSASILQTLMKDGCVLSRIAEDKFIIVMPIAETIAQVMDCANEITDAFVNPILP
                     ADSIGSLFVTTIVGVATFPEDGRDATPF"
BASE COUNT         1370 a          748 c          802 g         1452 t
ORIGIN      
        1 cacctgggag agcgcctcca tggcatggag gaggtagcga gttcgatcct cgtcggctcc
       61 accattattg taaaaatctc gatatttaga tccccctcta gatatcgagt ttttttatta
      121 cttaaagctg tttatttgct aaaatgaaag tatgaaaaga aaaacatatt tacgcaacca
      181 aacaatttat caaatttatg cgcgtaatca caccgcagaa ggaacatttg tcgctttaat
      241 taaggaccta ccaagaataa aggagcttgg tgtcgacatt ttatacttgc tgccaattca
      301 ccctattggt gaaattgctc gtaaaggtga gctaggaagt ccttatgcga ttcgtgatta
      361 ctatttaatt aatccggaac acggtacact tgaagacttt caaactttca ttactttagc
      421 gcattcatac ggttttaaag taatgattga tatcgtgctt catcacacta gtcctgatgc
      481 cgagttatta gcggagcatc ctgaatttta ttactataaa aacggcaaac ttggcaacaa
      541 agttggcgac tggagtgata ttattgactt agactataat aatcgtgact tatgggatta
      601 tatggtagaa atgctcaaat actgggctag tttaggagta gatggttatc gcgctgatgt
      661 ggcgccactc gttccccttg cattttggga atatgcacgg caggaactag atgaagacta
      721 tcctgaactt atttggctta gtgaatcagt ggaaccgcat tttattacaa atttaacggc
      781 agcaggtcat gttgcgcatc acgataaaga aatgtatagt gcttttgata ttctttatga
      841 ctatgatgtc tataatgact taaaagccta tttagaaggt aaggcaccac ttaaacctta
      901 tcttgatggc gttcgttatc aagagcaaaa ctatccaaaa ggttatttaa aaattagaac
      961 gattgaaaat catgataccg tgcggattgc ttctttatgt aaaacagatc aaattttgcg
     1021 taatgtcact gcttggtcat ttttccaaaa tggtgtaggg tttatttatg gtggtcaaga
     1081 agttaaggct cctcatcgcc cgacactatt tgacattgat aaaattgatt taaatgttaa
     1141 agaccaagac tattatgact ttatgatttt acttgttgaa atgaagaaac acccagtttt
     1201 tgccgagatt tcttccttta atattattgg cgacggtgca gatgatgtaa ttgtttcaac
     1261 gcttgagtca agagttggag cggcacacgg tttctttaat ttagggctta aagaaaaaga
     1321 agttaaaaca gagttaaaag acggaagtta cactaatgtg ctgacgaaca ctgatatcgt
     1381 tgttaacgac ggtaaaataa aagttagtga acctattatc attattgaag aataaatttt
     1441 ttgcagtgtt aaaataaagt gcaggcagtc atggcggaat tggtagacgc gcaagtttca
     1501 gacgcttgta tcgcaagatg tgggggttca agtccctttg gctgcaccaa ttttaattat
     1561 tttaatgagt gtaaatataa aggaaatagg agaaaactat gcacaaagtt agtattagga
     1621 gtaaattaac acctcatcaa gtttttacca ttcctaatat cttatccttt ttccgcttac
     1681 tacttattcc gcttatcgtc tttctctatg ttggtaaaga actttatggc tggacgcttg
     1741 ttgtacttat catttcacta gtcactgata ttattgatgg tctagttgcc cgtaaattaa
     1801 atatggtgac tgattttggg aaattcatcg accctgttgc cgataaatta acccaattag
     1861 cggttctatt ttgtttagtg actagattcc cgctgatgtg gattccaatt agcataatgt
     1921 tattaaagga aatacttcaa ttcgctcttc gctttgtagt gtttagatat actggtgaag
     1981 ttcatagcgc tgagtggcat ggcaaagtag cgacatttct tatctatgta atgatgtcat
     2041 tacatgttat ttggtatgga ataacagatg tagcgtcaac tttaacaata acaattactt
     2101 cactatcaat gcttttatca tgcgtgcttt atacaatttc tagtgccgta atcattatta
     2161 atattaaaag aagaaaaaaa gataaagagc aacttaataa ttaataagca ataattaatt
     2221 agtgcattat atataaaatt aaaagtaagt gattattttt gttttaaaaa gtcttcaaga
     2281 tctttaggtg gaagtgggcg tgagtagtag tagtagcctt gaatttcatc agtgcctaaa
     2341 tctttcataa acgctgcctg ttctttggtt tcaacacctt cagttgtaat attagcgttt
     2401 aatgatttcg ctaaattaat aattgtttta gcaataacaa ctttcttcgg ttcgattgta
     2461 atattatcaa cgacgctttt atcaatttta attcggttaa atgggacaag ttgtaagcgg
     2521 tgtagtgaag aatatccttt accaaaatcg tcaatggcaa tgtgtaatcc cgcatcatta
     2581 agtcttgtta atttttcaat agtgtcggca aaatttttcg agagcaaact ttcagtaatt
     2641 tctagttcaa gatactgagg ttccacctta tgttttttaa gtaatttcaa tacattatcc
     2701 acaaagtcat cacggcggaa ttgcgctaca gagagattaa cggaaaagcg cagtggggca
     2761 aatccctttt tcactagtgc cttatgctgt ttaagtgatt ggtcaatgac ccataacccg
     2821 acttcgtgga ttaagccagt ttgttcaagc attgggataa aaacatttgg tcccactctt
     2881 tgcccttcgt cagtcgtcca tctaagtaat gactcaacac catttatttg tccgttatta
     2941 caattaattt gtggttggaa ttgtaaatta aactctttat tttttaaaga actaaaaagt
     3001 ttattagtta gtagtgtata ttcttcaata tgtgttttaa gttcttcggt actaaagact
     3061 actttactat cggatatttt cgcttcatag gcagcaatat cggcattttc taaaagggtg
     3121 tagcatctct cccatcttca gggaatgtgg caacaccaac aattgttgtc acaaatagcg
     3181 agccaatact atctgctggt aatattgggt ttacaaaagc atccgtaatc tcgttagcgc
     3241 aatccattac ttgggcgata gtttcggcaa ttggcataac gataataaat ttatcttcgg
     3301 caatacgcga aagcacgcac ccatctttca taagcgtttg aagaattgat gcggatttaa
     3361 ttacaacttg ctcacctgtt gcgtgtccga atgtatcatt aatcatccgt agattatcaa
     3421 gttccacatt aaagacgact aacttatcag cgtcatcgcg attaagtaat agctcttcta
     3481 aattattaat aagcatattg cggtttggta aattagttga tttatcaata tacgcaaaat
     3541 catatagttg tttttcataa attattttct tgcgtgcatc acctaataca ttagcaataa
     3601 tttttaaata gtgtaaacga ctttcgacaa cggttagttc gtttttttct ttatattcaa
     3661 caacaaagaa accacttagt ttttcttcaa cgaatattgg taaagcatag aaagaattaa
     3721 tgcctctaga caggaagaaa ttggcacttt catcttgtgt atgagtacct aaatttgcaa
     3781 tatcatcaca gaagattggt gaatgtttac ctgcgagcgg ttctacttgt ggaaactcgc
     3841 tgattttaaa agaacgtcct aactgcagaa gaagtgaatt gtcctcacta tctcttacat
     3901 atgcattaag aattgttgct ctttcgcctt tgtcatcaaa tcgaaacaaa taagcttgat
     3961 catactgtaa tatttcggcg gccatcataa acatttcatc aattttagct cggacattgt
     4021 cttgattaac agcgatgaaa tttgtggaaa ttttttcaag aatttcttgt cccctcgaga
     4081 atctttgata accttgaaac tttgaatcat attcactagt aacataacgc acagccacgt
     4141 atgaaagtat gataataaat agccgcttag catactgcgc attatcaagg acaatagtaa
     4201 cattaggaaa tgtaatccat aacacaatct gtgtgaaaac tgtaaaaatt aagaagaata
     4261 atgtgtcgcg tttactatca agaacaattg aatatagtaa gaagacaata taaactgccc
     4321 acactgttga tgcccccata tgcgcatctt taacgatgaa gaaggtcata cc
//
LOCUS       DAOPCD010000060         4352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_16603_length_4352_cov_2.622527, whole genome shotgun sequence.
ACCESSION   DAOPCD010000060 DAOPCD010000000
VERSION     DAOPCD010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16603_length_4352_cov_2.622527"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PLQ55_02305"
     CDS             <1..802
                     /locus_tag="PLQ55_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPK28798.1"
                     /translation="NDPTNILSRKTLDELNLIVKQHGVNFLGVLKYDGTAFSGSLAKN
                     ITSSHTEVAEALGLAANDVVLLGFDAPLARILSALGAVRKELGVRFNLIDENAYSCLW
                     VDSFPLFSYDPETRRFASEHHPFTQPKEEDIAKLTTAPEEVLSYAYDIVINGYEAGGG
                     SMRIYDQDLQNRIFEILGLSEKEIEYLFGFFIEALKYGTPPHGGIAFGLDRLTMILTG
                     TDDIRDVIAFPKNLKGASLMSGEPSKVSNLQLDELYIKINTGKDGGEE"
     gene            806..3052
                     /gene="tkt"
                     /locus_tag="PLQ55_02310"
     CDS             806..3052
                     /gene="tkt"
                     /locus_tag="PLQ55_02310"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPK28799.1"
                     /translation="MCLRKRNTYDALSVAAIRATVIDITNTANSGHPGMAIGSAPLVY
                     NLYKNHLKIDPRNPEWIARDRFVLSAGHASALLYTVLHLAGYAIEIDDLKAFRQLNSI
                     TPGHPEYKMTPGVDATTGPLGQGLANAVGMALAEASLTARNPKYAHLLSHYTYCLVGD
                     GCLQEGISQEAVSFAGHQKLNKLIVFYDANDVTLDGPLSDSFSENVEARFKASGWNVL
                     KVDNGNNLKQIDKAIKKAKRSDKPTLVIMKTIIGQGSRQQGTHKVHGSPLGELDGTFA
                     KGTYHYPNAPFEIPLPVYESMRSQIARNGQKAYDTWAKSYTLFEADSDVEAEDLKIAL
                     NDDTESSLAMMKLTFEDELSEATRNTSGNVLAAVNRHLPHIMGGSADVASSVMTLING
                     ETNFTAKNRRGRNINFGIREFAMGAISNGMLLHGGLRTYVGTFLVFSDYLKNSMRLAA
                     LSHLPNIYLFSHDSVNLGEDGPTHQPVEHLAMMPAMPNFDLWRPGDARETFVAWKVAL
                     ESKTRPHALILTRQKLPLMAGSDKEKLIRRGAYVVSPENKRKKLDFIIIATGSEVNMA
                     LKAQNTLLIHGYNVRVVSMPSMEVFRRQDDAYKAEVLPLGKDKRIAVEILSSFGWHEW
                     ADHTMCLDRFGFSAPQKDILEHLKYTPEMLARLVEGIIDPKTLKKGEELYSGEAPNPI
                     PPVLSDLDTPAEGEELLTLEDVELKVSEKVEPEVTASETKEKQEATEAKAEVKTEAKP
                     KTKKTTKK"
     gene            3269..3592
                     /locus_tag="PLQ55_02315"
     CDS             3269..3592
                     /locus_tag="PLQ55_02315"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HPK28800.1"
                     /translation="MAEYVYIQKYNKQGIMGISYQVFDKIAEIATNEVSGATVSRKRY
                     SLSKPVRCTIRSGTVNVKISVKVSPNTNAEEVANEVRENVALSLTQMTELIPFDIDVE
                     VIDVG"
     gene            3601..4086
                     /locus_tag="PLQ55_02320"
     CDS             3601..4086
                     /locus_tag="PLQ55_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28801.1"
                     /translation="MMKEKSRINPKKIVFKLINFFLLLIIFSNLIGNFFGISSDYTYY
                     HWGLNSIAIFSRYIENSVLEIGMRIFLLGLTTMVSSALLFLLYHFARKGKIVPIVLAI
                     IIYVIDYSLVFSPWYRESTFSYIWSSFIHLIMIALFMAAGLFLLLLNPGGNKEYYDFT
                     E"
     gene            4070..>4352
                     /locus_tag="PLQ55_02325"
     CDS             4070..>4352
                     /locus_tag="PLQ55_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28802.1"
                     /translation="MISRNKSHEYGVQSVYAMLVFIKEDIAFDLPAVVSGVTGAEYSE
                     LPVFLKETLINIVKNYSEIVTNLTPYLRDWKFERLNLVTQAILIYSYAVY"
BASE COUNT         1401 a          760 c          907 g         1284 t
ORIGIN      
        1 taatgacccg acaaacatct tatcgcgtaa aacacttgat gaacttaact taatcgttaa
       61 gcaacacggg gttaatttct taggtgtttt aaagtatgac ggcaccgctt ttagcggtag
      121 tcttgcaaaa aacattacta gcagccacac tgaagtagca gaggctttag gtttagcggc
      181 aaatgatgtc gtcttacttg ggtttgatgc cccgctcgcg cgtattttaa gtgcactagg
      241 cgcagttaga aaagaattag gcgttcgctt taacttaatt gatgaaaatg cttactcctg
      301 tttatgggtc gattccttcc cactttttag ctatgatcct gaaactcgtc gcttcgctag
      361 tgaacaccat ccctttacgc aaccaaaaga agaagatatt gctaaattaa cgacagcacc
      421 agaagaagtt ttaagttatg cttatgacat cgttattaat gggtacgaag ctgggggcgg
      481 aagtatgcgg atttatgacc aagatttaca aaatcgtatc tttgaaatct taggcttaag
      541 tgaaaaagaa attgagtatt tattcggttt ctttatcgaa gcactaaaat acggcacccc
      601 accgcacggc gggattgctt ttgggttaga tagactgacg atgattttaa cgggtactga
      661 tgatattcgt gatgtaattg ccttccccaa aaatttaaag ggcgcaagtt taatgagcgg
      721 tgaaccttca aaagtttcta atttacaatt agatgaatta tatattaaaa taaatacagg
      781 aaaagatgga ggagaggaat aatttatgtg tttaagaaaa cgtaatactt acgatgcctt
      841 aagtgtcgcg gcgattcgtg cgactgttat tgacattact aataccgcta attcgggaca
      901 ccctgggatg gcgattggga gtgccccatt agtttataat ctttataaaa atcatttgaa
      961 aattgatccg cggaatccag aatggattgc ccgtgaccgt tttgtgctta gtgcgggaca
     1021 tgcgagtgcc ttactttata ctgtgctcca tttagcaggt tacgccattg aaattgatga
     1081 cttaaaagcc tttagacaac ttaactcgat taccccaggc caccctgaat ataaaatgac
     1141 accaggagtc gatgccacaa caggaccact cggtcaagga cttgcgaatg ccgtggggat
     1201 ggcgctagcg gaagctagtt taacagcgcg taatccaaag tatgcgcatc ttttaagcca
     1261 ttatacgtac tgtttagtgg gcgatggctg tttacaagaa ggaattagtc aagaagcagt
     1321 ctcgtttgct ggtcatcaaa aacttaataa attaattgtg ttttatgacg ctaacgatgt
     1381 gacgcttgac ggaccactga gcgattcatt tagtgaaaat gttgaagctc gctttaaagc
     1441 aagtggctgg aatgttttaa aagttgataa cggtaataat cttaaacaaa ttgataaagc
     1501 aattaaaaaa gccaaacgca gtgataaacc aacacttgta attatgaaaa cgattatcgg
     1561 tcaaggtagt cggcaacaag gtacacataa agtccacggc tcaccacttg gtgaattaga
     1621 tggtactttt gctaaaggta cttatcacta tccaaatgcg ccatttgaaa tcccgcttcc
     1681 agtatatgaa agtatgcgaa gccaaatcgc gcgtaacggc caaaaggcgt atgacacctg
     1741 ggcaaagagt tatacattat ttgaagccga cagtgatgtt gaagccgaag acttaaaaat
     1801 cgccttaaac gatgatactg aaagtagtct agcaatgatg aaacttacat ttgaagacga
     1861 attaagtgaa gcaacacgca acactagtgg taatgtactc gctgctgtta atcgtcattt
     1921 accgcatatt atgggcggaa gtgctgacgt tgctagtagt gtaatgacac taattaacgg
     1981 tgaaacaaac tttacggcta aaaaccgtag aggacggaat atcaatttcg ggattcgtga
     2041 attcgcgatg ggagcgatta gtaacgggat gctccttcac ggcggactac gcacttatgt
     2101 agggacattc ctcgtcttta gtgattacct taaaaactca atgcgtcttg ctgcactaag
     2161 tcacttacca aatatttatt tatttagtca tgatagtgtg aatttaggag aagacggacc
     2221 aacccatcaa ccggttgaac acttagcgat gatgcccgca atgccaaact ttgacttatg
     2281 gcggccgggt gacgcacgcg aaacatttgt ggcgtggaaa gtagccctag agagcaaaac
     2341 gcggccacac gccttaatct taacacggca aaaattacca cttatggctg gtagtgataa
     2401 agaaaagcta atccgtcgtg gtgcttatgt tgtaagccct gaaaataaac gaaagaaact
     2461 tgattttatt attatcgcaa cgggtagtga agtaaatatg gccttaaaag cgcaaaatac
     2521 tttactcatc cacggttata atgtccgtgt tgtctcgatg ccgagcatgg aagtgttccg
     2581 tcgtcaagat gacgcttata aagctgaagt attaccactt ggcaaagata aacgcatcgc
     2641 tgttgaaatc ttaagtagct ttgggtggca cgaatgggca gatcatacga tgtgcttaga
     2701 tcgcttcggc tttagtgccc cgcaaaaaga tattttagag cacttaaaat atacaccaga
     2761 aatgctcgca cgccttgttg aagggattat tgatccaaag acacttaaaa aaggtgaaga
     2821 actctattcc ggtgaagcac ctaatccaat cccgcccgtt ttaagtgatc ttgacacccc
     2881 agcggaaggt gaagaacttt taacactaga agatgttgaa cttaaagttt cagaaaaagt
     2941 tgaacctgaa gtaaccgcta gtgaaacaaa agaaaaacaa gaagcaactg aagcaaaggc
     3001 cgaagtaaaa actgaagcaa aaccaaaaac aaagaaaaca actaaaaaat aatcaattag
     3061 tagtttatta gtagttaatt ttaattggat tacttagaat ggcgcattgc aaatatgcgc
     3121 cattttataa ttctcactat ataaaattta ttttttaaat tgttaaatta acttattcaa
     3181 ttatcattca ataataaatt attggtcaaa agcaacaaat attgtaaaaa ggtgtttgtt
     3241 tcgttataat taaagccaga ggttatttat ggcagagtat gtttacattc aaaaatacaa
     3301 taagcaaggc ataatgggta ttagctatca agtattcgat aaaattgccg agattgctac
     3361 taatgaagtt agtggggcaa cggtttcacg taaacgttat tcgctttcta aaccagtacg
     3421 ttgtacgatc cgtagtggta ctgttaatgt gaagatttcc gtaaaagtta gtccgaatac
     3481 aaatgctgaa gaagttgcaa atgaagtccg tgaaaatgtc gcgcttagtt taacgcaaat
     3541 gaccgaatta attccgtttg acattgatgt tgaagtaatc gatgtcggtt aagcattata
     3601 ttgatgaaag agaagtcacg aataaatccg aaaaagatcg tttttaagtt aattaacttt
     3661 tttttattat taatcatttt tagtaactta attggtaatt tctttggcat cagtagtgat
     3721 tatacgtatt accactgggg attaaacagt attgcgattt tttcgcgtta cattgaaaat
     3781 agtgtcttag aaattgggat gcgcatcttt ttattaggct taacgacaat ggtaagcagc
     3841 gctcttttat ttcttttgta tcattttgcg agaaaaggta aaattgtacc gatagtgtta
     3901 gcaattatca tttatgtaat tgattactca ctagtgttta gtccgtggta ccgtgaaagt
     3961 acctttagtt atatatggtc gagctttatt catttaatta tgatcgcatt atttatggct
     4021 gccggactat tcttattact actaaatcca ggaggaaata aggaatacta tgatttcacg
     4081 gaataaaagt catgagtatg gtgtgcaaag tgtctatgca atgctcgttt tcattaaaga
     4141 agacattgct tttgatttac ctgcggttgt aagcggtgta actggtgcag aatatagtga
     4201 actccccgta tttcttaaag aaacattaat taacatcgtt aagaactata gtgaaattgt
     4261 gacgaactta acaccttatt tacgcgactg gaagtttgaa cgtttaaatt tagtaaccca
     4321 agccatctta atttatagtt acgctgttta tt
//
LOCUS       DAOPCD010000061         4327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_16718_length_4327_cov_4.728230, whole genome shotgun sequence.
ACCESSION   DAOPCD010000061 DAOPCD010000000
VERSION     DAOPCD010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16718_length_4327_cov_4.728230"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLQ55_02330"
     CDS             <1..256
                     /locus_tag="PLQ55_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HPK28803.1"
                     /translation="EYIEIEYKGEMPLKVVTEINELPGILEVIHTKEKLVIKTSEDDK
                     AFTKIIHILENNNVDYSRANANKPSLNDVFLELTGKELRD"
     gene            258..1424
                     /locus_tag="PLQ55_02335"
     CDS             258..1424
                     /locus_tag="PLQ55_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK28804.1"
                     /translation="MKSLFILNLKKLLKNKEIVFWALLFPIILSTIFHIVFDGIRNRD
                     NFETLKVNYVSTNFTAEDKAFLVEEMLTNKAEYIKNEGTDKAQTVKIFALSEKTKAEA
                     EKEFKKDKTIYFYKEDGAFKIKASSQNISTAILQTFIDDYQNFIKVTTLLVAESNGTL
                     SFQDAMNKHVASYAYTYDFDSGDKKIDYVNNYHYTVLAMAIIYGAFLATEQAKIFQPN
                     SFAFAKRVTVSGASRAKLLVSALLSSLVVQTAIIALYIAILVFIFKVTFANIGLALLA
                     ALIGVILVNINGFFIGIVFNKLSQNAVIAIIILAGTIGGFLSGMMISSMKFFVGTKMP
                     LLSYININALISDSFVSLDFGNMTRYWNNMLAMTVIGVILFGVTLIKFNRESNK"
     gene            1426..2661
                     /locus_tag="PLQ55_02340"
     CDS             1426..2661
                     /locus_tag="PLQ55_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK28805.1"
                     /translation="MGFKLFFKIVKANFVSFLAYVLILVVSGLMFGLSDSGAANEDFK
                     ILAPVYYANITNETVDDLPSDNTLIFDYLMDNNLISEESDDPFSQGFYTYTLNFVNVK
                     KNIKPSEVNDAIYAGVVRGALIIPKDLSTLTRENALFIFHTNSQDLATITPTKAALNK
                     FINTYHDLKEVSATAGNNLSATELTNKTLIALEKETEIVYENPNRTKLSMLSHFFSFS
                     TYIMSAVIILLVSIVMYEIKRREILMRISITPYSIPRLIVGLIFGAITLSLLLVGLVY
                     VLAIISYPKIALSITGLYLTLNIMLFTLPLTALAMVIGLVISKAEIVSIISTVFALAQ
                     AFFSGAFLPTFLLPKSIINVGKVFPASHTVTINNLLAEGLNTNGPSILLNIGILLAYF
                     AVFLTIAIILSKKITKREG"
     gene            2813..3268
                     /gene="rplM"
                     /locus_tag="PLQ55_02345"
     CDS             2813..3268
                     /gene="rplM"
                     /locus_tag="PLQ55_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPK28806.1"
                     /translation="MLRQTTHANAATVERKWYVVDATDLPLGRLASEIAMTLMGKNKP
                     EYTPHVDCGDYVIVVNADKVSLSGNKLKTKKYYNTSQRPGGLRVRTAGEMLANYPVEM
                     MERAVWGMVPKGRLGRQIIKKLFVYAGPEHKHSAQQPVELPLRYIGGSK"
     gene            3268..3666
                     /gene="rpsI"
                     /locus_tag="PLQ55_02350"
     CDS             3268..3666
                     /gene="rpsI"
                     /locus_tag="PLQ55_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPK28807.1"
                     /translation="MAKRKAETYLGTGRRKSSVARIILKPGKGSIIVNDQDVNDYMPY
                     QTLVMDLKQPLTLLDVADKYDVIANVHGGGFTGQAGAIRLGIARALLEAGLDRGTLKR
                     AGMLTRDARAKERKKYGLKGARRAPQFSKR"
     gene            3796..3872
                     /locus_tag="PLQ55_02355"
     tRNA            3796..3872
                     /locus_tag="PLQ55_02355"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3830..3832,aa:Ile,seq:gat)
     gene            3978..>4327
                     /locus_tag="PLQ55_02360"
     CDS             3978..>4327
                     /locus_tag="PLQ55_02360"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HPK28808.1"
                     /translation="MKQELIKNNYYEAVNKEWIDAAVIPGDQPMMSAFLELHLDIEKL
                     LMDLAKKWDKDQTGLNNHLQEFVKLYKMTNDFATREKLGAEPLKPVIKKILNLKSLKD
                     LEKNMKDFVLDGVD"
BASE COUNT         1449 a          708 c          797 g         1373 t
ORIGIN      
        1 ggaatacatt gaaattgaat ataaaggcga aatgccgcta aaagttgtga cagaaataaa
       61 tgaattacca ggtattcttg aagtgatcca cactaaggag aagttagtaa ttaagacgag
      121 tgaagacgat aaagcattta ctaaaattat tcatatttta gaaaataaca atgttgatta
      181 ttcgcgggct aatgcgaata aaccaagttt aaatgatgta tttttggagt taactggtaa
      241 agagttacgg gactaagatg aaaagtttat ttattttaaa tttaaagaaa ttacttaaaa
      301 ataaagaaat tgttttttgg gcgttattat ttccaattat attaagtact atttttcata
      361 ttgtgtttga tggtattcgc aatcgtgata actttgaaac acttaaagta aattatgttt
      421 ccacaaactt tactgccgag gacaaagcgt ttttagttga agaaatgctc actaacaaag
      481 ctgaatacat taaaaacgaa ggcacagata aagcacaaac agtaaagatc tttgcactta
      541 gcgaaaaaac taaggctgaa gccgaaaaag aatttaaaaa agataagacc atttattttt
      601 ataaggaaga tggggcattt aaaattaaag catcttcaca aaatataagt accgcgatat
      661 tacaaacatt tattgatgac tatcaaaact ttattaaggt tacgacactc ttagtcgcag
      721 aaagtaatgg tacattatca tttcaagacg ccatgaataa acatgttgca agctatgctt
      781 atacatatga ctttgattct ggtgataaaa aaatagatta tgttaataac tatcactata
      841 cagtacttgc gatggcgata atttatgggg catttcttgc gacagaacaa gctaaaatct
      901 ttcaaccaaa tagttttgct tttgctaaac gcgttacagt tagtggcgct agtcgcgcta
      961 aattattagt gagtgcctta cttagttcac tcgttgtgca aacagcaatt attgcgcttt
     1021 acattgcaat attagttttc atttttaaag tgacatttgc taatattggt ttagcgcttt
     1081 tagctgcatt aattggtgtt attttagtga atataaatgg ctttttcatt gggattgtct
     1141 ttaataaact tagtcaaaat gcagtgattg ctatcattat tttagcgggt actatcggcg
     1201 ggttcctctc agggatgatg atatcatcaa tgaagttttt tgttggcact aaaatgccgc
     1261 ttttgtctta tataaatatc aacgcattaa ttagtgattc atttgttagt cttgattttg
     1321 gtaatatgac tcgttactgg aataatatgc tagcgatgac cgttatcggt gtcattctct
     1381 ttggtgtaac gcttattaaa tttaatcggg agagcaataa ataagatggg gtttaaatta
     1441 tttttcaaaa ttgttaaagc aaacttcgtt tcatttctcg cttatgtttt aattttagtt
     1501 gttagtggtc ttatgtttgg gttaagcgat agtggcgccg ctaatgaaga ctttaaaatt
     1561 ttagcgccag tctactacgc aaatattaca aatgaaacag tcgatgactt accaagtgat
     1621 aacactttaa ttttcgatta tttaatggac aataatttaa ttagtgaaga aagtgatgat
     1681 ccgttttccc aaggctttta tacatacact ttaaactttg tgaatgtaaa gaaaaatatt
     1741 aagccaagtg aagtaaatga cgctatttat gccggagttg tgcgtggcgc actcattatt
     1801 ccgaaggatt tatcaacttt aacgcgggaa aatgcattat ttatttttca tacgaattcg
     1861 caagatttag cgacaataac cccaacaaaa gccgccctta ataagtttat taatacttac
     1921 cacgacttaa aagaagttag tgctacagcc gggaataact tatccgctac tgagttaaca
     1981 aacaaaacat taattgcgct tgaaaaagaa acggagatag tttatgaaaa tccaaatcgc
     2041 acaaaactgt cgatgttaag ccatttcttt tcattttcaa cttacattat gtcagcagtt
     2101 attatcttgc ttgttagtat tgtaatgtat gaaattaaac gccgtgaaat attaatgcgt
     2161 atttcgataa cgccctacag catcccgcgc ttaatcgtgg gccttatctt tggagcaata
     2221 accctttcgc ttctgttagt aggcctagtt tatgtcttag caattatttc ttatccaaaa
     2281 attgctttaa gcataactgg attatactta acacttaata ttatgctatt tactttacca
     2341 cttacggcac tagcgatggt cattggcctt gtaattagta aagctgaaat cgtctccatc
     2401 attagtacag tctttgcttt agcgcaagcc tttttctctg gagctttttt accaacattc
     2461 ctcttaccca agtcgattat taatgttggg aaagtctttc ccgctagtca tacagtgaca
     2521 attaataact tgcttgccga aggattaaat acaaacggac caagcatctt attaaatatc
     2581 ggtattttac tagcctactt tgcagtattc cttacaattg ccatcattct tagcaagaaa
     2641 attacgaaac gcgaaggtta atttcatctt tatgtcggct tatcaattat attgataaat
     2701 aaaataacga attaagttga caatatattt ataatatatt attattaatc acggcattat
     2761 ctgccttttg tcgtcccggt aagacgaaaa ttggtaaagg agaaatacaa atatgctacg
     2821 tcaaacaaca catgcgaatg ctgcaaccgt tgaacgtaaa tggtatgtcg ttgacgctac
     2881 tgatcttcca ctcggtcggt tagcgagcga aatcgccatg acgctcatgg gcaaaaacaa
     2941 accagaatat accccacatg ttgattgtgg agattatgtt atcgttgtta acgctgacaa
     3001 ggttagttta agcggcaata agctcaaaac aaagaaatat tacaacacca gccaacgtcc
     3061 tggcggttta cgcgtgcgga cagcaggtga aatgctcgca aattatcctg tcgaaatgat
     3121 ggaaagagca gtgtggggca tggttccaaa aggtcgtctt ggccgtcaaa ttattaagaa
     3181 attatttgtc tatgctggac cagaacacaa acatagtgca caacaaccag tggaactacc
     3241 cttacgttat attggaggaa gtaaataatg gcaaagagaa aagcagaaac ttacttaggt
     3301 actggccgtc gtaaatcaag cgtcgcacgc attattttga aacctggtaa aggttcaatt
     3361 attgttaatg atcaagatgt taatgattac atgccttatc aaaccttagt tatggactta
     3421 aaacaaccat taactttact tgatgttgcc gacaaatatg atgtcatcgc taatgttcat
     3481 ggtggtggct ttaccggtca agcaggggca atccgtttag gaattgcgcg tgctttactt
     3541 gaagctggcc ttgatcgcgg tacacttaaa cgcgcgggaa tgttaacccg tgatgctcgt
     3601 gccaaagaac ggaagaaata cggtcttaaa ggcgctcgtc gtgcaccaca attctccaaa
     3661 cgttaatata actacacgag aacaaacgaa agtctggttc aaaatgccag actttttctt
     3721 atattaaaaa cttttaatgt taaaaaagtt gcaatatgat caatgttgtt atattatata
     3781 ttagcgtccg cagatgggcc cttagctcag ttggttagag cgcacccctg ataagggtga
     3841 ggtcgatagt tcaaatctat tagggcccac catttgcaag taaagtcact actctgtagt
     3901 gatttttttg taaagaataa gtaaacttcc tctatttacc atacataaat gttaaaatac
     3961 gatggtgagg tgaaaatatg aaacaagaac taattaaaaa caattattac gaagctgtta
     4021 ataaagaatg gattgatgcc gcagtaattc ctggtgatca accaatgatg tcagcatttt
     4081 tagaactaca tttagatatt gaaaagttat taatggattt agcgaaaaaa tgggataaag
     4141 accaaacagg attaaataat catctgcaag aattcgttaa gctttacaaa atgacgaatg
     4201 attttgctac aagagaaaag ctcggtgctg aaccacttaa accagtaatt aagaaaatct
     4261 taaatttaaa gtcacttaaa gacttagaaa aaaatatgaa agattttgtg ttagatggag
     4321 tggattt
//
LOCUS       DAOPCD010000062         4293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_16898_length_4293_cov_3.206937, whole genome shotgun sequence.
ACCESSION   DAOPCD010000062 DAOPCD010000000
VERSION     DAOPCD010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16898_length_4293_cov_3.206937"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1946
                     /gene="dnaK"
                     /locus_tag="PLQ55_02365"
     CDS             126..1946
                     /gene="dnaK"
                     /locus_tag="PLQ55_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPK28809.1"
                     /translation="MAKKEIIIGIDLGTTNSVVSYRQADGQIKVIPTPEGKPTTPSVV
                     AFKPDGEQVNGEAAKRQAVTNPDTVMSVKRHIGTNKKFKINALKKEFTPEEISARILG
                     YMKDYAEKNLGQSIQKAVITVPAYFNDAQRQATKVAGEIAGLEVVRIINEPTAAALAY
                     GLDKEHDEKILVFDLGGGTFDVSILEIGDGTFEVIATAGDNHLGGDDWDAEIVEWIKQ
                     EIEREHGLKLNDKMAMQRFREAAEKAKIDLSQMLETTISLPFIGMGASGPISYEGKLT
                     RAKFLDLTKHLLARVEEPIRRALSDAKVKASDLNQVILIGGSTRMPAVQELVKKMTGK
                     APNLTVNPDEAVSIGAAIQGAVIAGDVKDVVLLDVTPLTLGIETMGEVMTPLITRNTT
                     IPTTKSQIFSTAADNQPSVDIVVYQGERPMARDNKLLGHFRLDGIKPARRGQPRIEVT
                     FDIDVNGIVKVTAKDLDTQKEQHITITNSSGLSKEEIDRMVKDAEEHKEADEKRKKNV
                     ELKNRAEQYINEIEHSLEEGGDKVDANQKEASEKLVTELKEAIEKEDYETLEKRLSEL
                     EQMAQMMQNMQQGQAEGETSATETPKDDDIIDADFTDKKD"
     gene            2014..3126
                     /gene="dnaJ"
                     /locus_tag="PLQ55_02370"
     CDS             2014..3126
                     /gene="dnaJ"
                     /locus_tag="PLQ55_02370"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPK28810.1"
                     /translation="MAKRDYYEVLGIKKSASKDEIKSAYRKLAKKYHPDVNKEKDAEE
                     KFKEVQEAYDVLYDDQKRATYDQFGHAAFDQNGGNPFGGGFSGSGFGFDFGDIFEGFF
                     GGGRRASRQTGPARGNDLLTRVRINFMDAINGTKIEFMHTYDKMCETCQGKGAENAND
                     IHTCSKCNGSGVETFVQESIFGRVQSQRACTSCGGSGKTISKPCKSCNGAGYTRVKEK
                     QLLNIPAGIGEGQKLRIQGKGERGLNGGPNGDLHVEIVIKPHNSFKRVGNDIYLQIPI
                     SFVDAALGVTLEVPTVYGNVEVKIPAGTQPGATLRLSGQGVKDVRGNVGNQYLEVEVK
                     TPTNLSKDQKVALEDFKKAEPKKETFFEKFKSKFKR"
     gene            3405..>4293
                     /locus_tag="PLQ55_02375"
     CDS             3405..>4293
                     /locus_tag="PLQ55_02375"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28811.1"
                     /translation="MLSKATLAFFALSALLISAISLGSNHGSSARGEPTPYAIVLGTE
                     KNKIAEGSENPDGYTGTGVLTTELGNNIAVSYKHLTNPSTHWQRIKTGGYIVNSDPIN
                     GITNLELTKRDDTVNVEILYSNTMSFDASRKVVINASSPTNFSTDFNGYKPKYIGIYA
                     LADSSFNLGKIEFDCVDHYHTLSVLSKNDARGTVTSEGLFRAGTNVTVTATALAPYFF
                     AGWFDASNNLVSSSATYTFAMPSNNLVLSADFVAEGEEMKFGAYPQTRVTDVTLITTL
                     NNLAGGSEGLPTAENAKAWT"
BASE COUNT         1471 a          754 c          951 g         1117 t
ORIGIN      
        1 gcttaattcg ccccgcgcaa gttatagtgt caacgaatat taaagaagaa ccaaaaaatg
       61 atgataatgt cccagaagtt aatcctgagg acttaaatta ataaaaagga gaagagatat
      121 atattatggc aaagaaagaa attattattg gaattgactt aggaacaact aatagtgtcg
      181 taagctatcg ccaagctgac ggccaaatta aagttattcc aaccccagaa ggcaaaccga
      241 ccaccccaag tgtcgtcgcc tttaaacctg atggtgaaca agttaatggt gaagcggcca
      301 aacgtcaagc ggttactaat cctgatactg tgatgagtgt taagcgtcac atcgggacaa
      361 ataaaaagtt taaaattaat gcgttaaaga aagaatttac accagaagaa ataagcgcga
      421 gaattcttgg ttatatgaaa gattatgccg aaaagaattt agggcaaagt attcaaaaag
      481 ctgttattac cgttcctgct tactttaatg atgcgcaacg gcaagcaaca aaagtcgctg
      541 gtgaaattgc cggccttgaa gttgtgcgga ttattaacga accgaccgca gcagcacttg
      601 cgtacggttt agataaagaa cacgatgaaa agatcttagt gtttgactta ggtggtggaa
      661 catttgacgt ttcaatctta gagattggtg atggtacctt tgaagttatc gcgaccgctg
      721 gagataacca cttaggtggg gacgactggg acgcggagat tgtcgaatgg attaagcaag
      781 aaattgaaag agaacatggc ttgaagttaa atgacaaaat ggctatgcaa cgcttccgtg
      841 aagcggctga aaaagcaaag attgacttaa gtcaaatgct tgaaacaaca attagtttac
      901 cctttattgg tatgggcgca agcggaccaa ttagttacga aggtaaatta acaagagcga
      961 agttccttga tttaacaaaa cacttattag cgagagtcga agaaccaatt cgccgggcac
     1021 taagtgacgc taaagttaaa gcaagtgacc ttaaccaagt aatcttaatt ggcggtagta
     1081 caagaatgcc tgctgtccaa gagttagtaa agaaaatgac aggtaaagca cctaacctta
     1141 ctgttaatcc tgatgaagca gtctcaattg gagcggccat tcaaggtgca gttattgctg
     1201 gagatgttaa agacgttgtc ttacttgacg ttactccgtt aaccttaggt attgaaacaa
     1261 tgggtgaagt aatgactccg cttattacac ggaatacgac tatcccgacg actaaatcac
     1321 aaatctttag tacggcagcc gacaatcaac catcggtgga cattgttgtc taccaaggtg
     1381 aaagaccaat ggcgcgtgat aataaattat taggtcactt ccgccttgac ggcattaaac
     1441 cagcgcgtcg cggtcaacca cggattgaag taacatttga cattgatgtt aacggtattg
     1501 ttaaagtaac cgccaaagac ttagacacgc aaaaagaaca acatattacg attactaata
     1561 gcagcggact ctctaaagaa gaaattgacc gcatggtaaa agacgctgaa gagcataaag
     1621 aagctgatga aaagcgcaag aaaaatgttg aacttaaaaa ccgcgctgaa caatatatta
     1681 atgaaatcga acatagttta gaagaaggtg gcgataaagt tgacgctaat caaaaagaag
     1741 caagtgaaaa actcgtcact gaactaaaag aagcgattga aaaagaagat tacgaaacgc
     1801 ttgaaaaacg tttaagtgaa ctcgaacaaa tggcacaaat gatgcaaaat atgcaacaag
     1861 gccaagccga aggtgaaact agcgctactg aaacaccaaa agatgatgac atcattgacg
     1921 ctgatttcac tgataaaaaa gactaattta aattaagaaa caactttcag gaaccccgcc
     1981 atccggggtt tctgtatgta aaaggagctg ccgatggcta aacgtgatta ttatgaagta
     2041 ttaggaataa agaaaagtgc ttcgaaagat gaaatcaaaa gtgcttatcg taaacttgcg
     2101 aagaagtatc atcctgatgt gaataaagaa aaggatgcag aagagaagtt taaagaagta
     2161 caagaagctt acgacgtcct ctatgacgat caaaaacgcg ctacatatga ccaatttggt
     2221 cacgctgcct ttgatcaaaa cggaggtaat ccttttggtg gtggctttag tggcagtggc
     2281 ttcgggtttg actttggcga tatttttgaa ggcttctttg gcggcggacg acgcgctagt
     2341 cggcaaactg gacccgctag agggaacgac ttattaacgc gggtacgcat taactttatg
     2401 gatgcgatta atggcacaaa gattgaattt atgcacactt atgacaagat gtgtgaaacg
     2461 tgtcaaggta aaggcgctga aaacgcaaac gatatccata catgtagtaa atgtaatggc
     2521 agcggggttg aaacctttgt ccaagaaagt attttcggcc gtgtgcaaag tcaaagagcg
     2581 tgtacatctt gtggtggcag cgggaaaaca attagtaaac cgtgtaaatc atgtaatggt
     2641 gcaggctata cccgtgtaaa agaaaagcaa ttacttaaca tcccagccgg cattggtgaa
     2701 ggtcaaaaac tacggattca aggcaagggt gaacgcggac ttaacggtgg tcctaatggt
     2761 gacctacatg ttgaaattgt cattaaaccg cataatagtt ttaagcgcgt tggtaacgat
     2821 atttatttac aaatccccat tagctttgtc gatgcagcgc ttggggttac acttgaagtc
     2881 ccaacggttt atggcaatgt cgaagttaaa ataccagcgg gtacgcaacc tggcgctact
     2941 ttacgcttaa gtggccaagg ggttaaagac gtacgcggta atgttggaaa ccaatactta
     3001 gaagtagaag tgaaaacacc gactaattta agtaaagatc aaaaagtggc gcttgaagac
     3061 ttcaagaaag cggagccaaa gaaagaaaca ttctttgaga aatttaaatc taaatttaaa
     3121 cgctaaaatt tattaaaatt atatgaaaga ggttaggaat aaatccctag cctttttttg
     3181 tataaaaaac attatcacaa aaaattgaat ttttgcttta gttaataatc aactaaatta
     3241 ttacgaatat atgcaattaa tttattttaa gtgaagtttt aatccttctt tttactaatt
     3301 taggtcatga aattaaaact tttaattgat tttatggtgt taaactaaat ttatgaacat
     3361 atgttcattt ggtcggaagg aaataatcta tgaaaaacaa atagttgctt agtaaggcga
     3421 ccttagcctt ttttgcgtta tcggcactac ttattagcgc gattagtctt gggagtaatc
     3481 acggctctag tgcgcgcggt gaaccaacac cgtacgccat tgttcttggt actgagaaga
     3541 acaaaatagc ggaaggatca gaaaatcctg atggttatac gggaacagga gtgcttacaa
     3601 ccgaactagg caacaatatt gctgtttctt acaagcattt aacaaaccct tcgactcact
     3661 ggcaaagaat taaaacgggt ggttatattg tcaatagtga cccgattaac gggattacga
     3721 atctagagtt aacaaaacgc gatgacactg taaatgttga aattctttat tccaacacaa
     3781 tgtcatttga tgcttcgcgt aaagtagtga ttaacgctag ttcaccaacg aactttagca
     3841 cggactttaa tggttataag cctaaatata tagggattta tgcgcttgca gattcctcct
     3901 ttaacttagg caaaattgaa tttgactgtg ttgatcacta tcatacactt agtgtgctta
     3961 gtaaaaatga tgcgcgtggt acagtcacga gtgagggctt attccgcgct ggaactaatg
     4021 tgacagtaac ggcaactgca cttgctccat acttctttgc ggggtggttt gacgctagta
     4081 ataatttagt gtcaagctct gcaacttata ccttcgctat gccaagtaat aatcttgtgt
     4141 tatccgctga ttttgtcgca gaaggggaag aaatgaaatt tggtgcttac ccgcaaacaa
     4201 gagtaacgga tgtaacgctt attactacac taaacaattt agcaggcgga agtgaaggat
     4261 taccaactgc cgaaaacgcg aaagcgtgga ctg
//
LOCUS       DAOPCD010000063         4268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_16998_length_4268_cov_3.257774, whole genome shotgun sequence.
ACCESSION   DAOPCD010000063 DAOPCD010000000
VERSION     DAOPCD010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16998_length_4268_cov_3.257774"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="PLQ55_02380"
     CDS             <1..1037
                     /locus_tag="PLQ55_02380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK28812.1"
                     /translation="KKAPNPSNQNFHNKLINILRSKFPLGAFSTRPMEKNIFLTQSKS
                     DYFYYPGYINTPVLKRLGIITAGLQYYEARTPKVIFVDTLNVTLLELARRLKKRHNVK
                     VIGIVTDNPINITGASKNYSQDVFKKSLVCDGFISLTQGLLRLFNPKQKPAIIVPGFI
                     EEAESFTNKKEKYAFFAGALYPKYGVDALIKTFRKLNPPFKLVIAGHGPLADDLLENK
                     HPNIEFLGQVCPPVAFRLAQRAYVNINPRPIDEQVDLYSVPSKVIDYINSGSVTISTE
                     NTEIRNLVGDNIYFIKDAEPHTILEALNAINKNYNYYKQRAAEAKKALVKALDNKVLI
                     NKIEDLINKI"
     gene            1080..2573
                     /locus_tag="PLQ55_02385"
     CDS             1080..2573
                     /locus_tag="PLQ55_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPK28813.1"
                     /translation="MQRKEMRAPVVFTLIRNVTIALLSFISFPFASQALGTAAMGVYN
                     WANTFVYYFLIISRLGIPLIAIRECAKVKDDKDKLSEKVQSFFVLQLITTALSFVLLS
                     TIMFIGKDTPMFKGDTWELIFLLSLNFLVGVFSFEWVYIALDRVFYMSVRSLATTILA
                     TLLIIVFVQNDHDLFIYAFINLIATFVTVTLNLLLLKREGVKLSFRRKVNLKHLTKSL
                     LFVFGITFLITAYNQNDSLLLGYLDDSFRTSGAYSVGIRGIEIVITIITSLSGIFVIR
                     ATSALKNDDMPAFHRVISYSTNITFFIGVPAVLFMIGLSPEIVGFIVKDSPYWDEAAI
                     NNAILAVVLLSSLMLTYSIHDGIYQQVLVPLKKEKIYFFTMLFVLILNIVASVLLALF
                     VFKDNPLFAVALVTLLSEFVVLIILFITAKEYVIRHIFSLNNLKIILSAGVSLGLVML
                     IKHFLPAMAYLATVGLCIFIGGIIYIVMLLLLKEDLVYAQVKGVKNG"
     gene            2566..3486
                     /locus_tag="PLQ55_02390"
     CDS             2566..3486
                     /locus_tag="PLQ55_02390"
                     /inference="COORDINATES: protein motif:HMM:NF025663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HPK28814.1"
                     /translation="MANPNFKENKLKRLIAKSIQPLLRIFSKKTYVSWQYRYITGHKL
                     DWKNLKRYTEKLQYLRLYYYPRNASVVKAASRDGARERVKSKGLEKLLIPIVGIYDKV
                     EDIPFEALPKRFVVKATHACALNHICLDKDTLDVNALKNKMRRWLKMDYGKKTVEPHY
                     SKIKPRLIVEHYVGKSDSLPTEYKMHVFNGKARYLYVVSGRGKDIRYDNLYVDFTPFP
                     ESQFNHWSSSDEVPRKPKNYEELVKHAEMLASDLLFCRVDFFIVDDHIYFNEFTFTPA
                     KGTLIFDDDKADFIISEWFDISSAKKNKAA"
     gene            complement(3458..>4268)
                     /locus_tag="PLQ55_02395"
     CDS             complement(3458..>4268)
                     /locus_tag="PLQ55_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28815.1"
                     /translation="YEISDRININQPLDNAFDNAIRNDLVNQNLSLEEAIKLYGSAET
                     KAIKKEDFRNEIEYMEAIFNSLSSAQNDKASWINYAANNNLVKGKYSKAIQESFKRGI
                     FSKLTDGIIKCDGTGPKVRMQISEQGMGQTFEHELISYNRFVLSARGGTSVDYGSYGK
                     ARVTKAKVKINISFFIEKSTTNSASKHTVTFIVPEMHSDNEAILFTSIISFDLREVLG
                     AETLKRVNGLMISYELLEHEFLKPGGVLDTSLNEEFALMLYEVMLPYSSWR"
BASE COUNT         1327 a          721 c          724 g         1496 t
ORIGIN      
        1 ataaaaaagc tcctaatcca agtaatcaaa actttcataa taaattaatt aatattttac
       61 ggagtaaatt tccgcttggt gcctttagta cacgcccgat ggaaaagaat attttcttaa
      121 cgcaaagtaa aagtgattat ttttattatc ctggttatat taatactccc gttcttaaac
      181 ggcttggcat tattactgct ggattacaat actatgaagc acgaacacca aaagttatat
      241 ttgttgatac attaaatgtg acgcttcttg aattagcgcg tagattaaag aaaagacata
      301 atgttaaagt tattgggatc gttactgata acccaataaa tattactggt gcaagtaaaa
      361 actatagcca agatgtattt aagaagagtc tcgtgtgtga tggcttcatt agtttaactc
      421 aaggactctt acgccttttt aatccgaaac aaaaaccagc aattattgtt ccgggcttta
      481 ttgaagaggc tgaaagtttc accaataaaa aggaaaaata cgcctttttt gctggagcat
      541 tatacccaaa atacggagtt gatgctttaa ttaaaacatt ccgtaaactt aatccgcctt
      601 ttaaattagt catagcggga cacggcccgc ttgcggatga tttacttgaa aataagcatc
      661 ctaatattga gttccttggt caagtttgtc cgcctgttgc ctttcgttta gcgcaaaggg
      721 cgtatgtcaa tattaatccg cggccaatag atgaacaggt tgatctctat tcagtaccaa
      781 gtaaagttat tgattacatt aatagcggaa gtgttactat ttccactgaa aacacggaaa
      841 ttagaaactt ggtaggggat aatatttact ttataaaaga cgctgaacca cacacaattc
      901 ttgaagcact taacgctatt aacaaaaatt ataactatta taaacaaagg gccgctgaag
      961 ctaaaaaagc tttagtaaaa gcacttgata acaaagttct tattaataaa attgaagatt
     1021 taattaataa aatctagtaa atccgccatt attcattaaa aatatcatat aatttactta
     1081 tgcaacgaaa agagatgcgc gcccctgttg tttttacact aattcgtaat gtaacaattg
     1141 cgctcctttc ttttatttct tttccctttg cctcacaagc acttggtact gcggcgatgg
     1201 gggtatataa ctgggcaaat acttttgtat attacttttt aattatctcg cggctcggga
     1261 ttccgcttat tgcgattagg gaatgcgcaa aggtgaaaga tgataaagat aaactaagcg
     1321 aaaaagttca gtctttcttt gtgctgcaat taattacgac agcactttct ttcgtgcttt
     1381 tatccacaat tatgtttatt ggtaaagata caccgatgtt taaaggtgat acttgggagt
     1441 taatcttttt attatcacta aacttcctcg tgggcgtctt ctcctttgaa tgggtatata
     1501 ttgctctaga tcgagtattt tatatgtctg ttcgttcttt agcgacaaca attttagcta
     1561 cattactgat tattgttttc gtacaaaatg atcacgattt atttatttat gcttttatta
     1621 acttaatcgc tacttttgta actgtgactt taaaccttct cttattaaaa cgcgaaggtg
     1681 taaaactttc ctttagacga aaggttaatt taaaacactt aacaaagtcg cttctctttg
     1741 tatttggtat tactttctta attaccgctt ataaccaaaa tgattcttta ctccttggtt
     1801 acttagatga ttcttttaga acatctggtg cttattctgt tggaattcgc ggtattgaaa
     1861 ttgttattac cattattact tctttaagtg gcatctttgt gatccgcgct acttctgcac
     1921 ttaaaaatga tgatatgcct gctttccatc gtgttataag ttacagcact aatattacct
     1981 tttttattgg tgtacctgcg gtcttattta tgattggctt atcaccggaa attgtcggct
     2041 ttattgttaa agatagtcct tattgggatg aagcagcgat taataacgcc attttagcgg
     2101 ttgttctgct ttcatcttta atgctaactt acagtattca tgacggtata tatcagcaag
     2161 tgcttgttcc gcttaaaaaa gaaaaaatat acttctttac tatgttattt gtgcttattt
     2221 taaatatcgt tgcaagtgta ttacttgcct tattcgtgtt taaagataat ccgctttttg
     2281 cggttgcgct tgttacactg ctttccgaat ttgtagtatt aattatttta tttattaccg
     2341 caaaagaata tgttattcgt catatattta gtcttaataa tctgaagatt attttatctg
     2401 caggtgttag tcttggttta gtaatgttaa taaaacactt cttaccagca atggcttact
     2461 tagcgactgt ggggctttgt atattcatcg gcggaatcat ttatatcgtt atgttactat
     2521 tattaaaaga agatttagtg tacgctcaag ttaaaggagt aaaaaatggc taatcctaat
     2581 tttaaagaaa ataagttaaa acgcttgatt gctaaaagta ttcaaccgct tttacgtata
     2641 ttttccaaga aaacatatgt gtcgtggcag tatcgttata ttactggtca taaattagac
     2701 tggaaaaatt taaagcgtta tactgaaaaa cttcaatact tacggctcta ctactatccg
     2761 cgtaacgcaa gtgttgttaa agctgcttcg cgtgacgggg cacgggaaag agtaaaaagt
     2821 aaggggcttg aaaaactttt aattccaatt gtggggattt acgataaagt tgaagatatc
     2881 ccgtttgagg cattaccaaa gcgctttgtc gttaaagcga cgcacgcttg tgctttaaat
     2941 catatttgtt tagataaaga cacacttgat gtaaacgcac taaagaacaa aatgcgtaga
     3001 tggttaaaaa tggattacgg taaaaaaaca gtggaaccac attactcaaa aataaagccg
     3061 cgcttaatag tggagcatta tgttgggaaa agtgatagtt taccaactga atataaaatg
     3121 catgtcttta acggtaaggc ccgctactta tatgtcgtta gtggacgcgg taaagatata
     3181 cgttatgata atttatatgt cgattttaca ccttttcctg aatcgcaatt taatcactgg
     3241 tcaagtagtg atgaagtgcc aaggaaacct aaaaactatg aagagcttgt aaagcatgcg
     3301 gaaatgctcg ctagtgattt attattctgt cgcgttgatt tttttattgt tgatgatcat
     3361 atttacttta atgaatttac ttttacacct gcaaaaggaa ccttaatttt tgatgatgat
     3421 aaggctgact ttatcattag tgaatggttt gatatttcta gcgccaagaa gaataaggca
     3481 gcataacttc atagagcatt aatgcgaact cttcattaag tgaagtatca agtactccac
     3541 ctggtttcag aaattcatgt tctaataatt cgtaacttat cattagtccg tttactcttt
     3601 taagtgtttc cgcacctaat acttcgcgga gatcaaaact aataatactc gtaaataaaa
     3661 ttgcttcatt atcactgtgc atctcaggga caataaatgt aacggtgtgt ttagaagcac
     3721 tgtttgtcgt gcttttttca ataaagaaac taatattaat ttttacttta gctttagtga
     3781 cgcgcgcttt accgtaactt ccataatcaa cacttgtacc accgcgagcg cttaacacaa
     3841 aacggttata actaataagt tcatgttcaa aagtttgacc catgccttgt tcactaattt
     3901 gcatcctaac ttttggacca gtgccgtcac acttaataat accgtctgtt aacttcgaaa
     3961 aaattccgcg tttaaatgat tcttgaattg ctttactata ttttccttta acaagattat
     4021 tattagcggc gtaattaatc cagctcgcct tatcattttg tgcactgctc aaactattaa
     4081 agattgcttc catatattca atttcattac ggaagtcttc tttttttatc gctttagttt
     4141 cagcactacc gtataactta attgcctctt caagtgataa attttggtta actaagtcat
     4201 ttctaattgc attatcaaaa gcattatcaa gtggttgatt aatatttata cgatcactaa
     4261 tttcataa
//
LOCUS       DAOPCD010000064         4263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_17027_length_4263_cov_4.807510, whole genome shotgun sequence.
ACCESSION   DAOPCD010000064 DAOPCD010000000
VERSION     DAOPCD010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17027_length_4263_cov_4.807510"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /gene="fusA"
                     /locus_tag="PLQ55_02400"
     CDS             <1..1533
                     /gene="fusA"
                     /locus_tag="PLQ55_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPK28816.1"
                     /translation="KTYIYDDNIANVSIEEVPEEYLEEMHIAKQNLIEKLAAFDDDFM
                     IQVLEGETPSVSEIKRVIRKAVLTGEFFPVFCGSAYKNKGIQPLLDGVVDYLPSPLDV
                     PPAKAELLDGTEIITPSDADAPLAALAFKIATDPFVGRLAYVRVYSGTIKAGSYVYNS
                     IKGVRERISRLVLMHSNHREEVSELKAGDIGAVVGFKNTTTGDTICDEKNKLILERME
                     FPEPVIEQAIEPKTRADQDKMTNGLAKLMEEDPTFKVTTNPETGQTIIAGVGELHLDV
                     LVDRLRREFGVNVNVGAPMVGYRETISQEAECEGKYIKQSGGRGQYGHVWIRFEPNPG
                     KGFEFVDAIVGGVVPREYIKPTEEGLKDALVRGLVAGYQVIDIKATLYDGSYHDVDSS
                     EAAYKIAASMALKEAGRLAEPVLLEPIMKVEITVPEQYYGDVLSDLARRRGSMKDESQ
                     RGNAKVIDAEVPLSEMFGYVTDLRSFTQGRGTYTMTFDHYGECPRYIADEIVKKSGTS
                     AR"
     gene            1642..2826
                     /gene="tuf"
                     /locus_tag="PLQ55_02405"
     CDS             1642..2826
                     /gene="tuf"
                     /locus_tag="PLQ55_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009007562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPK28817.1"
                     /translation="MAKERFDRSKVHVNVGTIGHVDHGKTTLTAAITKVLASQGGAKL
                     MAYDQIDRAPEEKERGITINTSHIEYQTATRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGVMPQTREHILLARQVGVPYVVVFLNKVDLVDDPELVDIVEMEVRELLNE
                     YGFPGDDTPIVRGSARDALDGKADAEKAIIELMEAVDSYIPDPERLTDRPFLLPIEDV
                     MTISGRGTVVTGRVERGMVKLNEPVEIVGIRPTKSTVVTGLEMFRKLLDSAQAGDNVG
                     VLLRGVARDEVERGQVLAKPGSVKPHTKFTSTVYVLTKEEGGRHTAFLSNYRPQFYFR
                     TTDITGVVTLPADVQMVMPGDTVTLTVELIHPVAIENGTKFSIREGGRTVGAGTVTEI
                     LK"
     gene            2938..4050
                     /locus_tag="PLQ55_02410"
     CDS             2938..4050
                     /locus_tag="PLQ55_02410"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPK28818.1"
                     /translation="MKKEKKITQPNLDTVPGGALKRTGAVLIDLVIMYLLMNVLWFAI
                     HPIYNKVYDIDNVVTAHERDKKRSNLVVLNEPILDDESNIDLVKVESNQLPETKRGEG
                     VFKFYTVFLRSEQEAKLAAGEIDNTPENNAMFTFDWYLENVIKPDTEESIYLRIPTDE
                     HLGAEGAVSSEETAEPEYDTFRPAGLDFKADLTAEDINKFNNRIYNQAVNAFNLRPNM
                     TIINKVNIQENIILMVLGSTVVFLVFPLFFKHGQTLGKKALKLGVTTRHGYTVTVPWL
                     LLRYFAFLFINLLSNLLIPIILPFISLTIMTFSKERRSAHDFIANTKVIDLQNSTVYK
                     SEAEFLEAQKRYNPRLEAEDFNEEVFSERFDEESGA"
     gene            4050..>4263
                     /locus_tag="PLQ55_02415"
     CDS             4050..>4263
                     /locus_tag="PLQ55_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28819.1"
                     /translation="MNHYVVKQTAGKRFLAGFLDTIFVIILALFLYIPARTIVDNNGL
                     IEVFVKITQIQLKSGLYDVEGIDNAVP"
BASE COUNT         1349 a          832 c          898 g         1184 t
ORIGIN      
        1 aaaacatata tttatgatga taatatcgct aatgtttcaa tcgaagaagt tccggaagaa
       61 tacctagaag aaatgcacat tgcaaaacaa aacttaatcg aaaaacttgc cgcctttgat
      121 gacgacttca tgattcaagt cttagaaggc gaaactccaa gtgtttcaga aatcaaacgc
      181 gttattcgta aagccgtctt aactggtgaa ttcttcccag tattctgtgg tagtgcttat
      241 aaaaacaaag gtattcaacc actgcttgac ggcgttgtcg attacttacc aagtccgtta
      301 gatgtcccgc cggcaaaagc tgaactactt gatggtactg aaattattac cccaagtgac
      361 gctgatgcac cacttgcggc cttagccttt aaaatcgcga ccgacccatt tgtgggacgc
      421 ctagcgtatg tccgtgtcta ttcaggtacg attaaagctg gatcctatgt gtataacagt
      481 attaaaggcg ttcgtgaacg gattagtcgt ttagtgctca tgcacagtaa tcaccgcgaa
      541 gaagtaagtg aacttaaagc tggtgatatt ggtgcggttg tcggatttaa aaacacgaca
      601 acaggggata caatttgtga tgaaaagaat aaattaatct tagaacgcat ggaattccct
      661 gaacctgtta ttgaacaagc aatcgaacca aagacccgtg ctgaccaaga caaaatgaca
      721 aatggacttg ctaagttaat ggaagaagat ccaacattta aggtgacgac taaccccgaa
      781 actggacaaa caattatcgc tggtgttggt gaacttcacc ttgatgtctt agtcgacaga
      841 ttacgccgtg aatttggtgt taatgttaat gtcggtgccc caatggttgg ctaccgcgaa
      901 acaattagcc aagaggcgga atgcgaaggc aaatatatta aacaatctgg tgggcgcgga
      961 caatatggtc atgtgtggat tcgctttgaa ccaaacccag gcaaaggttt cgaatttgtc
     1021 gatgcaattg tcggtggtgt ggtcccgcgc gaatacatta agccaaccga agaaggttta
     1081 aaagacgcat tagtgcgcgg tttagtcgct ggatatcaag taattgatat taaggctaca
     1141 ctgtatgatg gcagttatca cgatgttgat agtagtgaag ctgcttacaa aattgctgca
     1201 tcaatggcgc ttaaagaagc gggacgccta gcagaacccg tcttacttga accaattatg
     1261 aaagttgaaa ttactgttcc agaacagtat tatggcgacg tgcttagtga cttagcaaga
     1321 cgccgcggtt caatgaagga tgaaagccaa cgcggaaacg ctaaagttat tgatgctgaa
     1381 gtaccacttt ccgaaatgtt tggctacgtt actgatttac gtagtttcac ccaaggtcgt
     1441 ggtacatata cgatgacttt tgatcactat ggcgaatgtc cacgctacat cgcagatgaa
     1501 atcgtcaaaa agagtggaac tagtgctcgc tagtccgcaa atgctatata aatagcacaa
     1561 tgatttgata aaaaattaaa attgctataa actaatagcg ttgcaataaa ttaaaacgct
     1621 acaagttagg aggaaattat tatggcaaaa gaaagatttg acagaagtaa agtccacgtt
     1681 aacgttggta ccattggtca cgttgaccac ggtaaaacaa ccttaaccgc agctattacc
     1741 aaagtcctcg catcgcaagg cggtgcaaaa ttaatggcgt atgatcaaat tgaccgtgca
     1801 ccagaagaaa aagaacgcgg aattacaatt aatacttcac acattgagta tcaaaccgcc
     1861 actcgtcact atgctcacgt tgactgtccg ggacacgctg actatgttaa aaacatgatt
     1921 accggtgccg cacaaatgga cggagcaatt ctcgttgtgg ccgctactga cggtgttatg
     1981 ccacaaacac gtgaacacat cttacttgca cgtcaagttg gtgttcctta cgttgtagtc
     2041 ttcttaaata aagttgactt agtcgacgac ccagaactcg ttgacatcgt cgaaatggaa
     2101 gttcgtgaat tacttaacga atacggattc ccaggcgatg ataccccaat cgttcgcggc
     2161 agtgcccgtg atgcattaga cggtaaagca gatgctgaaa aagcaatcat cgaattaatg
     2221 gaagctgttg acagctacat tcctgaccca gaacgcttaa ccgatcgtcc attccttctc
     2281 ccaatcgaag acgtcatgac catcagtggt cgtggtaccg tcgtcactgg tagagtggaa
     2341 cgcggaatgg tcaaattaaa cgaaccagtt gaaatcgttg gtattcgtcc aactaaatca
     2401 accgtcgtta ctggccttga aatgttccgt aaattactcg acagcgccca agctggtgac
     2461 aacgttggtg ttttactccg cggtgtcgca cgcgacgaag tcgaacgtgg tcaagtctta
     2521 gccaaacctg gctcagttaa accacacaca aaattcacaa gcaccgttta cgtcttaaca
     2581 aaagaagaag gtggtcgtca cactgcattc ttatcaaact atcgtccaca attctacttc
     2641 cggacaactg acattaccgg tgttgtaaca ctaccagccg atgtgcaaat ggttatgcct
     2701 ggcgataccg tcaccttaac cgttgaactc attcacccag tcgccattga aaatggaact
     2761 aagttctcca ttcgtgaagg tggccgcact gttggtgccg gtactgttac cgaaatctta
     2821 aaataagatt taaagcatta cacaactaac tcctcttcag aggagttttt tgtttatgat
     2881 tatatcgtaa caattgaaat atatgatagt ttttagtagc atagtactta gcgaaatatg
     2941 aaaaaagaaa agaaaattac ccaacctaat ttagatacag ttccaggtgg cgctttaaag
     3001 cgtactggtg cagttttaat tgaccttgtc attatgtatt tattaatgaa tgtattatgg
     3061 tttgccattc atccaattta taacaaagtt tatgatattg ataatgttgt gaccgcacat
     3121 gagcgtgata aaaaacgtag taacttagtc gtccttaatg aaccgattct tgatgatgaa
     3181 agcaatattg acttagttaa agttgaaagt aatcaattac cagaaacaaa acgcggggaa
     3241 ggtgtcttta agttttacac agtctttttg cgcagtgaac aagaagcgaa attagcggcg
     3301 ggtgaaattg ataatacacc tgaaaataat gcaatgttta catttgattg gtacttagaa
     3361 aatgtcatta aacctgatac ggaagaatca atttatttac gcattcccac tgatgaacat
     3421 ttaggagcag aaggcgcggt tagtagtgaa gaaactgctg aaccggagta cgacactttt
     3481 cgtccagcgg gattagattt taaagcggat ttaactgcgg aagatattaa taaatttaat
     3541 aaccgcattt acaatcaagc agtgaacgct tttaatttac gaccgaatat gacaatcatt
     3601 aataaggtta atattcagga aaatataatc ttgatggtgc ttggtagcac tgtcgtcttt
     3661 ttagtgttcc cgcttttctt taaacacggt caaacactcg gcaaaaaggc cttaaaatta
     3721 ggagtaacga cgcgtcatgg ttacacagtt actgtgccgt ggttattact tcgttacttt
     3781 gctttcttat ttattaactt attaagtaat ctcttaattc caattatttt accgtttatc
     3841 tcactcacaa ttatgacctt tagtaaagag cgtcgcagtg cgcatgactt tatcgctaat
     3901 acgaaagtaa ttgatttaca aaattcgaca gtatataaaa gtgaagctga gttcttagaa
     3961 gcacaaaaaa gatataatcc tagacttgag gctgaagact ttaatgaaga agtatttagt
     4021 gaacgcttcg atgaagaaag tggcgcctaa tgaaccatta tgtcgtaaag caaacagcag
     4081 gtaaacgctt ccttgcgggt ttccttgata cgatatttgt cattatattg gcgctttttt
     4141 tatatattcc ggcgcgcact atagtagaca ataatggctt aatcgaagtg tttgttaaaa
     4201 tcacacaaat tcaattaaaa agcggactat atgacgttga agggattgat aatgcagtac
     4261 ctt
//
LOCUS       DAOPCD010000065         4248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_17106_length_4248_cov_3.457191, whole genome shotgun sequence.
ACCESSION   DAOPCD010000065 DAOPCD010000000
VERSION     DAOPCD010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17106_length_4248_cov_3.457191"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1119)
                     /locus_tag="PLQ55_02420"
     CDS             complement(106..1119)
                     /locus_tag="PLQ55_02420"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPK28820.1"
                     /translation="MYQIRAKRKYSNILIAPFGRRMKAALLDLLLVLVLGFFSFMAVD
                     AIYTNSKVGSTANLTFYNIKKDSGLYHSYDESRTTAFLNLDINERDNYVRYFLGLEYF
                     YTQENTGFIYEKSVYYTEDTEFNYRTMVLKEGEDDSFFDFDTPYKTISYSFKANLSAG
                     DRNEVWKNLYANALTDLMRTPAYQKAEKVLKQFIVLNLSISAFSGTILPILAAPFFFG
                     HGRTLGKFLTGLAVVDKNGYKVSWWRMLIRSLLYGVVEIGLNFYGFFMPLLLTSGAVA
                     LTHKRQALHGIFSGTYVVDARTSKIFNNINDEEEYFSLSPEERELNKNYYQEKPYVPL
                     KKL"
     gene            1208..1999
                     /locus_tag="PLQ55_02425"
     CDS             1208..1999
                     /locus_tag="PLQ55_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28821.1"
                     /translation="MEKSLQPTDTKKRRRRKRPRRIRQNWFFRFLRNFVKLFKKRPLI
                     YDHNEDIVGPAIFVSNHSGAAGPMDLSMFFPYYFVPWGAHEMRGNYRTRWKYLYYVFY
                     QQKLHYGKFKSFFLATILAVINPFLYANMRLIPTYQDIRFTKSLRESLTYLDDGIPIL
                     VFPEDSSEGYFDTIIKFNEGVVVLADYVDKHRNLNIPIYPVYYSKRAREIEIGKPAYY
                     RDLKAKGHTRSEIADILRKHINTLYENIKARKARNAKAKERVRKG"
     gene            complement(2065..>2771)
                     /locus_tag="PLQ55_02430"
     CDS             complement(2065..>2771)
                     /locus_tag="PLQ55_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPK28822.1"
                     /translation="VERKLGVKETKEKQTVRRLINSLIRFFFLFWIIIMILKELGLDL
                     VPLLAGAGVVAFAVGFGAQELIKDIIAGMFLILEHTFKIGDFVEIGGQSGTVVDVGLR
                     RLKIQNWKGEVITMNNGDIKVVMNASINPSIAIIEFKMDYPFNVKELETADFRAFLKK
                     FKDKNPQVLEMPEKIVLMDVNDGLLFRVTIKTESRQHIGIEREFRKELYKYVQAKNWP
                     IIVPVVVEEHDSSYQA"
     assembly_gap    2772..2781
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3030..>4248)
                     /locus_tag="PLQ55_02435"
     CDS             complement(3030..>4248)
                     /locus_tag="PLQ55_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28823.1"
                     /translation="TCATVVTITAHNSHAISVPSDAFTIICIDGVYYEFYSSPFEITY
                     TNFKVTDYELSWETNANFTEIKVGSDDPRVIPQTYISLPDYISGNAIHEFTFKGRVLL
                     KEGPSFTVKVISHDLKYYLYDDNLTLETPTLVRDDNIVTIIANGLYNSFEVSIGTHNF
                     TTTASNFDLLDYAEYMSGEAFTVSVKAAYNDILYSEAATLSLVYIDGVIYNSGEEPSI
                     TGFALKNGKVLLWDKVEGISEYDVQIGEYKKTVSTNEISLYNERNHIKRHTPVVIKAG
                     NLSGVCHINCDYETLSFSIEAVPKLNTPQGFNIEEKMYLTFARVPNDTANSLGMGSSY
                     YLYYNDGYSYETSSPAGASNTVSFNLGYISGWYTNPQVVRIVVHGHSDWASSEAALVT
                     INRLNHDHYEIIY"
BASE COUNT         1369 a          776 c          719 g         1374 t
ORIGIN      
        1 aatcgtaatc agtaggttgc gggttcaatt cctgtcgtcg gcaccagtca aaatgaaaat
       61 gtcccacgca tctgggactt ttttattaat taaaagtaat gtttattaaa gttttttaag
      121 gggaacgtag ggtttttctt gataataatt tttattaagt tcacgctctt caggacttag
      181 tgaaaaatat tcttcttcgt cattaatgtt attaaatatt tttgaggttc ttgcatcaac
      241 gacatatgtg ccactgaaaa taccgtgcag cgcttgtctt ttatgtgtaa gtgcaaccgc
      301 tccgcttgtg agtaaaagcg gcataaaaaa gccatagaaa tttaagccga tttcgacaac
      361 gccatatagc aaagagcgaa ttaacatacg ccaccacgat actttatagc cgtttttatc
      421 aacaacagca agtccagtca aaaatttacc aagcgttcgt ccgtgcccaa aaaagaaagg
      481 cgctgcaaga ataggtaata ttgtgccgct gaaggcactg attgataaat ttaaaacaat
      541 gaattgcttt aaaacttttt cggctttttg ataagcggga gtacgcatta aatcagttaa
      601 agcgttcgcg tagagatttt tccacacctc gtttctatca ccagctgaaa gattagcttt
      661 aaatgaataa gagattgttt tatatggtgt atcaaaatca aagaatgagt catcctctcc
      721 ttcttttaat accattgtgc gatagttaaa ttcggtgtct tctgtataat acactgattt
      781 ttcatagatg aaaccagtat tttcttgggt atagaaatat tctaaaccta agaagtaacg
      841 aacataatta tcacgctcat taatatctaa atttaaaaat gctgtcgtcc gcgattcatc
      901 ataactatga tataaacctg aatctttttt aatgttataa aaagttaaat tagcggtgct
      961 tcctacttta ctattcgtgt aaatagcgtc cactgccata aaactaaaaa agccgagcac
     1021 taacactaat aagaggtcta aaagtgcagc tttcatcctg cgcccgaagg gggcaattaa
     1081 tatatttgaa tatttccgtt tagcgcgaat ttgatacata acgatattat agcaaatatg
     1141 ccgcactaag tttaagattt ggtatcttcc tttgctaaaa gtacgcttat tcggtaaaat
     1201 gaacaatatg gaaaaaagtt tacaaccgac tgatacaaaa aaaagacggc gcagaaaaag
     1261 accacgaaga attaggcaaa actggttctt ccgtttttta cgtaattttg tgaagctatt
     1321 taagaagcgt ccgcttattt atgaccataa tgaagatatt gttggtcctg ctatttttgt
     1381 tagtaaccat agcggtgcag cgggtccaat ggatctttca atgtttttcc cttattactt
     1441 tgtgccatgg ggagcgcacg agatgcgtgg taactatcgt acacgctgga aatatcttta
     1501 ttatgttttt tatcaacaaa aattacatta cggtaaattt aaaagttttt tcttagcgac
     1561 aattcttgcg gtaattaatc ctttccttta tgcaaatatg cgcctaattc ctacttatca
     1621 agacattcgt tttacgaaga gtttacggga aagtttaact tatttagatg acggcattcc
     1681 tattcttgta ttccctgaag attcaagtga aggttatttt gacacgatta ttaaatttaa
     1741 tgaaggtgtc gtcgtgcttg ctgactatgt cgataaacat cgtaatctta acattccaat
     1801 ttatccggtt tattacagta aaagggcgcg tgagattgaa attggtaaac ccgcttacta
     1861 tcgtgactta aaagcaaagg gtcatacccg ctctgaaatc gcagatatct tacggaagca
     1921 tattaataca ttatacgaaa atattaaagc aagaaaagca cgaaacgcga aagcaaagga
     1981 acgagtaaga aaaggttaaa atgctcttat tataacgaca aaaggcggct agattgccgc
     2041 ttttttactt tgcttttaaa tagtttaagc ttgataactt gaatcgtgtt cttcgacaac
     2101 aactggcaca ataattggcc aatttttagc ttgtacatat ttataaagtt ctttacggaa
     2161 ttcgcgttca atgccgatgt gttgccgtga ttctgttttg attgtaactc taaagagtaa
     2221 accatcattt acatccatta agacaatttt ttcaggcatt tcaagtactt gtgggttttt
     2281 gtctttaaac ttctttaaga aggctctaaa gtccgctgtt tctaattctt taacattaaa
     2341 tggataatcc attttaaact caatgatggc gatgcttggg ttaatactag cgttcatcac
     2401 tactttaata tcaccattat tcatcgtaat tacttcacct ttccagtttt gaattttaag
     2461 gcgtcgtaaa ccaacatcta cgacagtgcc ggattgacca ccgatttcaa cgaagtcacc
     2521 aattttaaaa gtatgttcta aaataaggaa catccccgcg attatgtctt taattaattc
     2581 ttgggcacca aagccaacgg caaaagccac aaccccagca cctgctagaa ggggcactaa
     2641 atcaagtcct aattctttta agatcataat aataatccag aataaaaaga agaaacggat
     2701 taggctattg ataaggcgac gcactgtttg tttttctttt gtttctttaa ccccaagttt
     2761 acgttcaacg cnnnnnnnnn ngtgcggaaa ataattaatt taataacaag cgtaactaaa
     2821 taagcaagga tcacccaaaa gataatcata attgctgtta ctaaaatttg cgcaagtgat
     2881 tcatttgcga taaaagacgc gaaccagtta tttaatactt cacgtaattg tgctgctaaa
     2941 ttcatattga tatacctcca ttaacgcttg taattttagc gaaatatgcc acttttttcc
     3001 aatgttatgt tataaaatta aaagtttaat taatagatga tttcataatg gtcgtgatta
     3061 aggcgattaa tcgtcactag tgccgcttca ctgcttgccc aatcgctatg accatgcacc
     3121 acaattcgca ctacctgcgg attagtgtac catcccgaaa tatacccaag attaaaagaa
     3181 acagtgttac ttgcacctgc tggcgaagaa gtttcataac tataaccatc attgtagtaa
     3241 aggtaatatg aagaacccat gccaagcgaa ttagcggtat catttggtac tcttgcaaat
     3301 gtaagataca ttttttcttc aatattaaaa ccttgtggtg tatttaattt agggactgct
     3361 tcaattgaaa aacttagcgt ttcataatcg caattaatat gacaaacacc acttaaatta
     3421 cctgctttta taacaaccgg cgtatgtctc ttaatgtgat ttcgctcatt atataaactt
     3481 atttcattcg tggaaacagt ctttttatat tcaccaattt gcacatcata ttcactaatg
     3541 ccttcaacct tatcccacaa gagtactttc ccgtttttta aggcaaaacc agtaatactt
     3601 ggctcttctc cagagttata aattacgcca tcaatataca ctagtgaaag tgttgccgct
     3661 tcactataaa gaatgtcgtt atacgctgct ttaacactaa cagtaaatgc ttcgccactc
     3721 atatattcag cataatctag taaatcaaaa tttgatgctg ttgttgtgaa attgtgagtg
     3781 ccaatagaaa cttcaaagct attatataag ccattagcga ttattgtgac tatattatca
     3841 tcacgaacta atgtaggtgt ttctagcgtt aaattatcat catacaagta atatttaaga
     3901 tcatgactta ttactttaac agtaaatgat ggcccttctt ttaaaaggac acggccttta
     3961 aatgtaaatt catgaatagc gttcccactt atgtaatcag gaagactaat ataagtttgc
     4021 ggaataacgc gcggatcatc actgccaact tttatttcgg taaaattggc gtttgtctcc
     4081 cacgaaagtt cgtaatcagt cactttaaaa ttcgtgtatg ttatttcaaa tggggatgaa
     4141 taaaattcat aatatacacc atcgatacaa attattgtaa aggcgtcact tgggacactg
     4201 atagcgtgac tattatgcgc ggtaatcgtt accacagtag cgcaagta
//
LOCUS       DAOPCD010000066         4245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_17120_length_4245_cov_3.544391, whole genome shotgun sequence.
ACCESSION   DAOPCD010000066 DAOPCD010000000
VERSION     DAOPCD010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17120_length_4245_cov_3.544391"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..481)
                     /locus_tag="PLQ55_02440"
     CDS             complement(8..481)
                     /locus_tag="PLQ55_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28824.1"
                     /translation="MQIVRIIILVLVVIYLLLAFVFMHISLDYTRQLKKSKETIHSLF
                     AGQIALFAMIGKELESPNSEAQVMNELLEKREFTELNKLAAEKERAYQELAAKKKDTT
                     PQTAQLLQGLSENVVLIRNEIYRHNKLVDNINVNVDSVIFSLFVVILRLKRLTKI"
     gene            666..1334
                     /locus_tag="PLQ55_02445"
     CDS             666..1334
                     /locus_tag="PLQ55_02445"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPK28825.1"
                     /translation="MENIVRMPILGERFPELDTMTTFGKKKLPNDYEGKWFILFSHPG
                     DFTPVCTTEFIAFNEEIEEFRKMNAELIGLSIDQLQSHMKWTEFIGKITGKEIGFPII
                     ADELGRVANALGMVHPGKGTNTVRAVFIVDPKGVLRLMMYYPQEVGRAVYEVKRALNA
                     LQVADEHKVAAPENYPNNAFLGKDMVILPPPGTEKQKADRLALLERGEAKSYDWWFSY
                     KKLK"
     gene            1488..2321
                     /locus_tag="PLQ55_02450"
     CDS             1488..2321
                     /locus_tag="PLQ55_02450"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPK28826.1"
                     /translation="MGTAKPIIAIIYDFDKTLTTTDMQNYNFIPALGMTPEEFWGETG
                     KFSDKTGVERILSYMYMMIVKAREKGINLTKEFLYEQGKNMEYFDGVLTWFDRINEYG
                     EKKSIKIEHYLISSGTKEIIEGSEIAKHFKAIFGCEFYFDPQTKLPVWPKLAINYTAK
                     TQFYFRITKGILDVSEDNALNRKLVDHRIPHRNIIYIGDGMTDVPVMLLVKQNGGHSI
                     AVYPKGEREKVEYLYTDGRVNFVARADYTEKSDINSIVRLIIDQVSIAEQIEKKKQLL
                     N"
     gene            2324..2695
                     /locus_tag="PLQ55_02455"
     CDS             2324..2695
                     /locus_tag="PLQ55_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPK28827.1"
                     /translation="MNIYELNEIVEGTITEIRPYGAIITFPENRTGLLHIKQISDAYI
                     SNINTYLQLDAQIKVRIIEIDEHNGFLKLSLKSVPYNERIIFERDKKATNINPAQTDF
                     SALEKALPLWIEKAIKEAQND"
     gene            2688..3965
                     /locus_tag="PLQ55_02460"
     CDS             2688..3965
                     /locus_tag="PLQ55_02460"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPK28828.1"
                     /translation="MIRLNYDNVLTTIDFEKYRSKLEKINEGINQKKGAGNDYLGWAD
                     WPLHYDKKEFNLIKETALMIRETFDTLVVTGIGGSYLGARAAIEAINGLLPQKGPEII
                     FLGHTFSPTLTAQTLEYLKTKNFAINVISKSGTTTETSIAFRLLKQLLIEKVGEANAN
                     KAIFATTDAKTGALRKLATQNGYTTFTLPSDIGGRYSVLTAVGLLPLAVAGIDIDDLM
                     AGALKARSESVDNLDHDLYKYAVIRDTLYRQNYKSELYVVYEPQYAMFNEWLKQLYGE
                     SEGKEKKGLFPTSVTFSTDLHSLGQFIQDGSPILFETTLFVENPNQDVVIPSEKENLD
                     ELNYLAGKGLGFVNKQAFQGTLAAHVKDGGVPNLVFTTNKLDAYTLGYMFYFFMRSCA
                     VSAYLLDINPFNQPGVEVYKKNMFALLGKPKQK"
     gene            4049..4124
                     /locus_tag="PLQ55_02465"
     tRNA            4049..4124
                     /locus_tag="PLQ55_02465"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4082..4084,aa:Val,seq:tac)
     gene            4145..4228
                     /locus_tag="PLQ55_02470"
     tRNA            4145..4228
                     /locus_tag="PLQ55_02470"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4179..4181,aa:Tyr,seq:gta)
BASE COUNT         1430 a          749 c          774 g         1292 t
ORIGIN      
        1 tatttaacta gattttagta agtcttttta atcgtaaaat aacgacaaac aatgaaaata
       61 taacagaatc aacattaaca ttaatgttat ccactagttt gttatgacga tatatttcat
      121 tacgaataag tacaacattc tcactgagac cttgtagtag ttgcgctgtt tgtggagttg
      181 tatctttctt tttcgctgct aactcttgat aagcgcgctc tttttccgcc gctagcttat
      241 taagttcggt aaattcacgt ttttccaaaa gttcattcat tacttgtgct tcactatttg
      301 gtgactctaa ttctttacca atcattgcaa aaagggcaat ttgacctgca aaaaggctgt
      361 gaattgtttc tttgctcttt tttaattgtc gtgtgtagtc gagtgaaata tgcataaaaa
      421 caaatgctaa gagcaagtat ataacgacta gaaccaaaat aataatacgc acgatttgca
      481 tagtttaatt ataacataca acgattattg taaattagtg attttattta agattaatta
      541 tgaaagcgtt atattaactt ttttcaagaa taaaaatagc aacttaccaa aagaaagcat
      601 atttaaagaa gtttccatta aaaaaagata aaatatagct gtatacaaaa ggaggaaatt
      661 aatatatgga aaatattgtt agaatgccaa ttcttggcga acgcttcccc gagttagata
      721 caatgacaac tttcgggaaa aagaaattac ctaatgatta cgaagggaaa tggtttattc
      781 tcttctcaca cccaggtgac ttcacaccag tctgtacgac tgaatttatt gcttttaatg
      841 aagaaattga agaatttaga aaaatgaatg cggaacttat cggactttca attgaccaac
      901 ttcaatcaca tatgaagtgg actgaattta tcggtaagat tactggcaaa gaaattggat
      961 tcccaattat cgctgacgaa ctaggccgtg tcgctaatgc gcttggtatg gttcaccctg
     1021 gtaaaggcac aaatactgtt cgtgctgtct tcatcgttga tccaaaaggt gttttacgct
     1081 taatgatgta ctacccacaa gaagttggcc gtgcagttta cgaagtaaaa agagcactta
     1141 atgcgctcca agtcgctgat gaacataaag ttgctgctcc agaaaactac ccaaataatg
     1201 ctttcttagg caaagacatg gtcatcttac cgccaccagg aaccgaaaaa caaaaagcag
     1261 atcgtcttgc gttacttgaa cgcggtgaag caaagagtta tgactggtgg ttctcttata
     1321 aaaaattaaa ataagaattc ctataactac taaacaaggt gctacaaaac gcaccttgtt
     1381 tttgtttata atacttttca acgatttagt ggctatttat aagtccttat tcgttttgac
     1441 aatcactcta ttgagtgata ttatgttata aatggaggtt tttcaacatg ggtacagcta
     1501 aaccaattat tgctattatc tatgactttg acaaaacatt aaccaccaca gatatgcaaa
     1561 actataattt tattcctgcg cttggtatga caccagaaga attttggggt gaaacaggaa
     1621 aatttagtga taaaactggc gttgaacgga ttttatcata tatgtatatg atgattgtca
     1681 aagctcgtga aaaaggtatt aatttaacga aagaatttct ttatgaacaa ggcaaaaaca
     1741 tggaatactt cgatggggtc ttaacttggt ttgaccgcat taatgaatat ggtgagaaaa
     1801 aaagcattaa aattgaacac tatctcatta gcagcggcac aaaagaaatc attgaaggtt
     1861 cagaaattgc taaacacttt aaagccattt ttggttgtga attttacttt gatccacaaa
     1921 ctaaattacc agtatggcca aaactagcga ttaactacac cgctaaaaca cagttctact
     1981 tccgcattac taaaggtatc ttagatgtca gcgaagataa tgccttaaac cgtaaactag
     2041 tagaccatcg catcccacat cgcaacatta tttatatcgg cgacgggatg acagatgttc
     2101 cggtaatgct ccttgttaaa caaaatggcg gtcactcaat tgctgtttat ccaaagggtg
     2161 aacgcgaaaa agttgagtac ttatacactg atggccgggt aaactttgtt gctcgcgctg
     2221 attatacaga aaaaagtgat attaattcca ttgttcgctt aattattgac caagtgtcta
     2281 tcgccgaaca aattgaaaag aagaaacaac ttcttaacta gttatgaaca tttacgaact
     2341 aaatgaaatt gtcgaaggca ctattactga aattagacca tatggtgcca ttattacctt
     2401 tcctgaaaat aggactgggc ttttacatat aaaacaaatt agtgatgcct acattagtaa
     2461 catcaacaca tacttgcaac ttgatgcaca aattaaagtg cggataattg aaattgatga
     2521 acataatggt ttcttaaaac tttcacttaa atccgttcct tacaatgaac gcattatttt
     2581 tgaacgcgat aaaaaagcaa caaacattaa tccggcacaa actgatttta gtgcacttga
     2641 aaaagcattg ccgctttgga ttgaaaaggc tattaaggag gcgcaaaatg attagattaa
     2701 attatgacaa tgtattaact acgattgatt ttgaaaaata ccgctctaaa cttgaaaaaa
     2761 ttaatgaagg cattaatcaa aaaaagggag caggaaatga ttatcttggt tgggctgatt
     2821 ggccattaca ttacgataaa aaagaattta atttaattaa agaaacagcc ttaatgatta
     2881 gagaaacctt tgatacttta gtcgttactg gtattggtgg ttcttacttg ggggcaagag
     2941 ctgcaattga agcaattaac ggattattgc cgcaaaaagg acctgaaatt atctttttgg
     3001 gtcatacttt ttctccgacc ttaacagcgc aaacgctcga atacttaaaa acaaaaaact
     3061 ttgcgattaa tgttatttct aaaagtggta ccactactga aacaagtatt gcttttcgct
     3121 tattaaagca actattaatc gaaaaagtcg gtgaagcaaa tgctaataag gcaatttttg
     3181 cgactacaga cgctaaaact ggtgcgctcc gtaaactagc gacacaaaat ggctacacta
     3241 cttttacatt gccaagcgat atcggtggtc gttatagtgt tttaaccgct gttggacttc
     3301 taccacttgc tgttgcggga attgatattg atgacttaat ggcgggagct cttaaagcac
     3361 gcagcgaaag cgtcgataat ttagatcatg acctttataa atatgcagtt attcgcgaca
     3421 cactctatcg tcaaaattac aagagcgagc tctatgttgt ttacgaacca caatacgcta
     3481 tgtttaatga gtggttaaaa caactatatg gcgaaagcga aggtaaagaa aagaaaggtt
     3541 tattcccaac ttcagttacc ttctccactg atttacatag cttaggacaa tttattcaag
     3601 acggatctcc tattttattt gagacaacgc tctttgttga aaatccaaac caagatgtcg
     3661 tcattcctag tgaaaaagaa aacttagatg aattaaatta ccttgcgggc aaaggattag
     3721 gttttgtcaa taaacaagcc ttccaaggga cgcttgctgc gcatgtcaaa gatggcggtg
     3781 ttcctaacct tgtttttaca actaataaac ttgatgctta cactttaggt tacatgttct
     3841 acttctttat gcgatcatgt gccgtaagcg catatcttct agacattaat ccttttaatc
     3901 agcctggcgt tgaagtttat aaaaagaata tgtttgcctt acttggtaaa ccaaaacaaa
     3961 aatagaaccc cgaatttgat agtattattt gttgactaaa taactatcta atgatatatt
     4021 aagtaagcat aaattagcga ccagtcccgg atggttagct cagctgggag agcatcgcct
     4081 ttacacggcg gaggtcgggg gttcaaaccc ctcaccatcc accatttatt ttatgccaaa
     4141 agtcgggaga gtagcgaagt ggtcaaacgc ggctgactgt aaatcagttc ctgagggttc
     4201 ggtggttcaa atccgccctc tcccaccatc ttttgtttgg ggtat
//
LOCUS       DAOPCD010000067         4154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_17580_length_4154_cov_4.385216, whole genome shotgun sequence.
ACCESSION   DAOPCD010000067 DAOPCD010000000
VERSION     DAOPCD010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17580_length_4154_cov_4.385216"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..704)
                     /locus_tag="PLQ55_02475"
     CDS             complement(18..704)
                     /locus_tag="PLQ55_02475"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HPK28829.1"
                     /translation="MCRRINTNKIAYVSLVMLLALGLKASIPKKEFMANDHFIFEINH
                     RDKYATAIELTEAGKEQEVLVVPPSIRGFPVRYIGKPKRFFQPLDINALKLTDKQIKI
                     YLPYSLRGQMWLASVRTVEAILALANLDDFDKVLTINSFPEIKLHYLNGKNKLNTFYM
                     YNFESSLNEGYYFMDYINGSNPYVIPSDPVRKGYTFAGWYYQKECATLWNNEVPKSES
                     ESLTLFAKWI"
     gene            complement(952..1695)
                     /gene="tpiA"
                     /locus_tag="PLQ55_02480"
     CDS             complement(952..1695)
                     /gene="tpiA"
                     /locus_tag="PLQ55_02480"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPK28830.1"
                     /translation="MRKKLLVGNWKMNKTMAETAVFAHASFELLKNAKAKNIDIGVTP
                     PYLSLQTLLNINPKLIIGAQNVHHLEKGAYTGEISIPMLKELGVSWSLVGHSERRTYF
                     NETNESCNLKVLALLENNMTPLYCVGETLQEFEKGVTKEVIKAQIEVGLKGVKDVKEV
                     VIAYEPVWSIGTGKNASKEIAEDICAYIRELLRAKYGGDADLVRILYGGSVKPNNIKE
                     YMAMPNIDGALVGGASLSVESYAELINNM"
     gene            complement(1697..2914)
                     /locus_tag="PLQ55_02485"
     CDS             complement(1697..2914)
                     /locus_tag="PLQ55_02485"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPK28831.1"
                     /translation="MAKIIRDLKLANKTVLIRCDFNVPFKGEVISDDNRIRAALPTIK
                     FALDSGAKVVLFSHLGKIKHKGTEEEVEAAKKKNNMVIVFRRLAEILGEDKVFFSPET
                     SGEEVNKRVALLKPGQVLLLQNTRYEKGEEKNDPVLSEKWASFADVFVMDAFGSAHRA
                     HASTVGVPELLKARGKEVALGFLVEKEVEALGRVVHVAKKDRPYIAVIGGFKVSDKIK
                     VIETLLQKCDKVLIGGAMAYTFQVAQGHNIGISPFEPEQVDFAKKCLDTAGEKILLPV
                     DAVATDAFQDWTKKITTTDSNIPDGFEGMDIGPKTQKLFADELSKAKMIFWNGPMGVF
                     EQKDFEAGTRAVCEAVSKLKGAFTVIGGGDSAAAVAQFGYKDYFSHVSTGGGASLEMI
                     ENDGHLPGIDVIG"
     gene            complement(2976..4007)
                     /gene="gap"
                     /locus_tag="PLQ55_02490"
     CDS             complement(2976..4007)
                     /gene="gap"
                     /locus_tag="PLQ55_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPK28832.1"
                     /translation="MSIKIAINGFGRIGRLAFRRIAETKGMEVVAINDLTDAENLAYL
                     LKYDSAHGRFHVDDISVTADGTAIVFKGKEIPVYAQPDPTQLNWTKHGVDIVLECTGR
                     FRGKEDASVHLQEGVKAVIISAPADKETPMFVYGVNTDKLTKDMTVISGASCTTNALA
                     PVVKVLDDNFGVERGLMTTVHAYTNDQTILDLPKKKAARRGRAGAMNIVPTSTGAAKA
                     IGQVLPHLNKKLDGGALRVPVIDGSMVDLTVLLKKKVTAEEINAVMKKAAEGELKAIL
                     GYTEDEVVSGDIIGDTHGGLFDATLTKVMEVDGEQLVKVMFWYDNEYGYTCQYIRLAQ
                     EVAKVHGVK"
BASE COUNT         1206 a          883 c          689 g         1376 t
ORIGIN      
        1 agcatctctt agtttagtta aatccactta gcaaaaagcg ttaacgattc actttctgac
       61 ttaggcacct cgttattcca caaagtagcg cattctttct gataatacca tcccgcaaaa
      121 gtgtatcctt tacgaacagg gtcagaaggt atgacataag gattactacc attaatataa
      181 tccataaagt aatagccttc atttagggaa gattcaaaat tatacatgta gaaagtatta
      241 agtttatttt tcccgtttag ataatgtaat ttaatttcag gaaatgaatt aatagttaat
      301 accttatcaa agtcatctag attagccaaa gcaaggatag cttcgacagt cctcactgac
      361 gctaaccaca tttgcccacg caaggaatat ggaagataaa tctttatttg cttatctgtt
      421 aacttcagtg cattaatatc taagggttga aaaaatcgtt ttggttttcc tatataccga
      481 actggaaaac ctctaatgct tggaggcaca accaaaactt cttgttcttt tccggcctcg
      541 gttaattcaa ttgccgttgc atatttatct ctgtgattga tttcaaatat aaaatggtcg
      601 tttgccatga attctttctt gggtatactt gcttttaatc ctagcgcaag taacatgact
      661 aaacttacgt aagcaatttt attagtatta atccgtctgc acatagtttc tcccttacgc
      721 taaagaatag cgtcaccatt tgatcgaata atcattgacc actacatata aacaagtgac
      781 ttaaaaaata taatgtaatt ttatcatgag ttttatatac gctatcctct ttttataaat
      841 cacattttca tcatctttaa ctactgtata ttatttttcg acctcaaaag tcgctattta
      901 aaaagggaac cattttcttg gtcccctgta atttgcttta tgtagataac actacatatt
      961 attaataagt tcagcgtaac tttcaacact aagcgaggca ccgccaacga gggcaccatc
     1021 aatatttggc atcgccatat attctttaat gttgttaggt ttaactgagc cgccgtaaag
     1081 aattcgtact aaatcagcgt caccgccgta tttagcgcgt aataattcac ggatataagc
     1141 gcagatgtct tctgcaattt ctttacttgc atttttacca gtgccaattg accacactgg
     1201 ttcgtaagca atcacgactt ctttaacgtc tttaacacct ttaagcccca cttcaatttg
     1261 tgctttaatt acttcttttg tcacaccttt ttcaaattct tgtaatgttt caccaacaca
     1321 gtatagtggt gtcatattat tttcaagtaa agcgaggact tttaagttac agctttcatt
     1381 tgtttcgtta aagtacgtcc ttctttcact gtgaccgaca agtgaccaac taacacctag
     1441 ttcttttagc attggaatac taatttcacc agtgtaagcc cctttttcaa ggtgatgaac
     1501 attttgcgca ccaataatga gctttggatt aatatttaat aaggtttgca gtgataaata
     1561 aggtggggtg actccaatat caatattttt agctttggca tttttaagta attcaaagga
     1621 agcatgagcg aaaactgctg tttccgccat tgttttattc atcttccagt tgccaacaag
     1681 taatttcttc cgcatattat ccaatcacat caatgcctgg taaatgacca tcattttcaa
     1741 tcatttctag tgatgctccg ccacccgtgc ttacatggct aaagtaatcc ttataaccaa
     1801 attgcgcgac ggcggctgca ctatcaccac caccaatgac agtgaatgct ccttttaatt
     1861 tactcacagc ttcgcacaca gcgcgtgtac cagcttcaaa atctttttgt tcaaagacac
     1921 ccattggtcc gttccagaaa atcattttgg ctttgcttaa ttcatccgca aataattttt
     1981 gtgtttttgg cccaatatcc atcccttcaa aaccgtcagg aatattcgaa tccgttgtcg
     2041 ttattttctt tgtccagtct tggaaggcat cagtggcaac agcgtcaact ggtaataaaa
     2101 tcttctcacc agcagtgtct aaacatttct ttgcaaagtc gacttgttca ggttcaaacg
     2161 gtgaaatccc gatattatga ccttgagcga cttggaaagt gtaagccatt gccccaccaa
     2221 ttaatacttt gtcacatttt tgtaataaag tttcaataac tttaattttg tcactcactt
     2281 taaagccacc aataacggca atataaggcc ggtctttttt agcgacatga acaacacggc
     2341 caagtgcttc gacttctttt tcgactaaaa agccaagcgc aacttcttta ccgcgggctt
     2401 ttaagagttc aggaacaccg acagtcgaag cgtgagcgcg gtgggcacta ccaaaagcgt
     2461 ccatcacgaa gacatcagcg aaacttgccc acttttcact aagaacgggg tcatttttct
     2521 cttcaccttt ttcgtaacgc gtattttgta agagtaagac ttgtcctggt ttaagtaaag
     2581 caacacgctt atttacttct tcgccactag tttcagggct aaagaagact ttatcttcac
     2641 ctaaaatttc cgctagacgg cggaagacaa ttaccatatt atttttcttt ttagcggctt
     2701 caacttcttc ttcggttcct ttatgtttaa ttttaccaag gtgtgagaaa agcactactt
     2761 ttgcgccaga atcaagcgca aacttaattg taggtaaagc agctctaata cggttatcat
     2821 cactgatgac ttcgccttta aatggcacgt taaagtcaca gcgaattaac actgttttgt
     2881 ttgctaattt aagatctcta ataattttag ccatattttt tctccttttt tagataaaaa
     2941 gcgcccgaag gcgctttctt taaatcatta ataaattatt taacgccgtg aactttggca
     3001 acttcttgcg caagacggat gtattggcaa gtatagccat attcattgtc ataccagaac
     3061 attactttaa cgagttgttc accatcgact tccataactt tcgttaaggt tgcgtcaaat
     3121 aaaccaccgt gggtatcacc aatgatgtcg ccactgacga cttcgtcttc ggtgtaacca
     3181 agaatggctt ttaattcacc ttcagcagct tttttcatca cagcgttaat ttcttcagca
     3241 gtaacttttt tctttaagag cactgttaag tcaaccattg aaccatcgat tactgggaca
     3301 cgtaaagcac caccatcaag ctttttgtta agatgtggga gtacttgacc aattgcttta
     3361 gcggcaccgg ttgaagttgg aacgatattc atcgcacctg cccgaccacg acgtgcagct
     3421 tttttctttg gtaagtctaa gattgtttgg tcatttgtat aggcgtgaac agtagtcatt
     3481 aaaccgcgtt cgacaccaaa attgtcatca agcactttca cgactggcgc tagtgcgttt
     3541 gtggtacagc ttgcaccact aatgacggtc atatcttttg ttaatttatc ggtattaaca
     3601 ccgtaaacaa acattggggt ttctttatca gcaggagcac taatgatgac ggctttcaca
     3661 ccttcttgta agtgcacact tgcatcttct ttaccacgga aacgaccagt acattctaag
     3721 acgatatcaa caccgtgttt tgtccaattt aattgggttg gatcaggttg agcgtaaact
     3781 gggatttcct ttcctttaaa aacgattgct gttccatcag cggtcacgct aatgtcatca
     3841 acgtggaaac gaccgtgtgc gctatcatat tttaaaaggt atgctaaatt ttcagcatca
     3901 gttaagtcgt taatcgcgac aacttccatt ccttttgttt cggcaatccg acggaatgct
     3961 aagcgaccaa tccggccaaa tccattaatt gcaattttaa ttgacataat tttcctcctt
     4021 tatttatgcg tttatattat acaacattag ttatctaatg tactaacaaa aacatacaaa
     4081 aaaacgaata ttttcactta tgttagatga catacttatc tttacaaata aagattagga
     4141 aacttttaaa tttt
//
LOCUS       DAOPCD010000068         4145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_17623_length_4145_cov_3.364059, whole genome shotgun sequence.
ACCESSION   DAOPCD010000068 DAOPCD010000000
VERSION     DAOPCD010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17623_length_4145_cov_3.364059"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /gene="rsgA"
                     /locus_tag="PLQ55_02495"
     CDS             <1..559
                     /gene="rsgA"
                     /locus_tag="PLQ55_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPK28833.1"
                     /translation="KPLVIITKQDKLDTDDLLKEVQDNLDKLHVKNYVVNNKSGEGLD
                     AVRKYLHGKVVALMGQSGVGKSSFINALTDYDREVGKFSISINRGRHVTKEVIMLPFN
                     AGYLVDTPGFSSFELPLTKGDLAENFPGFKKYIGTCKFNNCLHLSEIGCTVKADVEAG
                     LISEESYNNYVMISDELKLYSEDYK"
     gene            556..1221
                     /gene="rpe"
                     /locus_tag="PLQ55_02500"
     CDS             556..1221
                     /gene="rpe"
                     /locus_tag="PLQ55_02500"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPK28834.1"
                     /translation="MKQKVYVAPSLLAADFKNLHRELLILQQNKVKYLHFDVMDGHFV
                     PNLSFGIPILEALKPHYIFIYDVHLMVTNPEFIAPKFVEAGADIVTFHLEAMKTGNDV
                     VTLIKELRLMGVKVGISIKPDTPVEDLAPILKDIDLVLVMSVEPGFGGQSFLRSALHK
                     IEWLDEERKKYKYNYLIEVDGGINAETGALCAEAGADILVAGSYLFGKEDINERIKGL
                     RNG"
     gene            1214..1867
                     /locus_tag="PLQ55_02505"
     CDS             1214..1867
                     /locus_tag="PLQ55_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28835.1"
                     /translation="MDKSIVHIAFFSSLSLFVITLIFQLSLYRTKQNRKFSFRNELPF
                     ELVQGADIKFINYHYVLLFLVTIANLLFAFKYLDHIYNWYEYLLVGSLVLSAIMLYLI
                     FFIKVFEIKKHIIVVILQALSVVTSYLSFGLFAHISPFGKQNIVFGIFGYLFALIGML
                     VLLNPRLRKWPIMDKVLQQDGTVLILRPRYFMLALYEWGFIAAQFLLMIVMYAYLYV"
     gene            complement(1917..2108)
                     /gene="rpmB"
                     /locus_tag="PLQ55_02510"
     CDS             complement(1917..2108)
                     /gene="rpmB"
                     /locus_tag="PLQ55_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPK28836.1"
                     /translation="MARHCPVTGKGPLSGNKRSHSLRATRRRWGVNLQNYKVEVDGKV
                     QTVRMSARAYRSLNKSAKV"
     gene            2275..3336
                     /locus_tag="PLQ55_02515"
     CDS             2275..3336
                     /locus_tag="PLQ55_02515"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HPK28837.1"
                     /translation="MDLINKNIGEKIKSRRLRLGYSQLMLSELAHVSRDQISRIESGK
                     INFTLATLFKICRALRIKPHKLLKDAEQEEYIKSGDLRPFVKWAGGKTQILDELMQRI
                     PNEFNNYFEPFVGGGAFFLKLEHPRSVINDINSELMTAYSVIKDKEKFIALKKLLLQY
                     EEKHNEEFYYEVRARDRDPSFSTLSDVEIAARFIYLNKNCFNGLYRVNSKGYFNVPFN
                     KSEKVKTFDEDNINKLHKYLSLPSIKICCEDYKNMTNKAKKGDFVYFDPPYDTLKDDT
                     FTAYDKEGFGRNEQIELSEEFKKLHKKGVKVMLSNHNTPLINELYKEFNITIIHARRA
                     INSRGSGRGKIEEVIITNY"
     gene            3338..4129
                     /locus_tag="PLQ55_02520"
     CDS             3338..4129
                     /locus_tag="PLQ55_02520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPK28838.1"
                     /translation="MNIINVGKSALKINDDDFILVQGNALRVLKKIAPKSVDVIFADP
                     PYFLSNDGITCVNGKMVSVNKALWDKSISATDKLNFNRKWIRYCRDALKDDGTIWISG
                     TMHNIYSIGVALELEGFSIINNITWRKPNPPPNIACRAFTHSTETVLWARKIINNRKK
                     GKHFFNYALMKEINGGKQMKDVWELKGALKKEKEHGKHPTQKPFELLERIILASSNEG
                     DIILDPFAGSGTTGIAAKRLKRRFIGIEIEKEYIGIIKKRYKAIQ"
BASE COUNT         1371 a          607 c          780 g         1387 t
ORIGIN      
        1 taagccacta gtcatcatta caaagcaaga taaacttgac actgatgact tgttaaagga
       61 agtacaagat aatcttgata aactacacgt taaaaattat gttgtcaata ataaaagtgg
      121 tgaaggactt gatgctgtgc gcaaatattt gcatggcaaa gttgtggcac ttatggggca
      181 atctggtgtc ggaaaatcaa gttttataaa cgctttaaca gattatgacc gcgaagttgg
      241 taagttctca attagcatta accgtggtcg acatgtcact aaagaagtaa taatgcttcc
      301 gtttaacgcc ggatatttag ttgatacacc aggcttttct tcctttgaat taccgctaac
      361 gaaaggcgac cttgccgaaa acttcccggg atttaaaaag tatattggta cttgtaaatt
      421 taataattgt ctccatctta gtgaaatagg ctgtaccgtt aaggccgatg ttgaagctgg
      481 gcttatcagt gaagaaagtt ataataatta tgttatgata agtgatgagt taaagttata
      541 tagtgaggat tataaatgaa acagaaagtt tatgttgccc catctttatt agctgcggat
      601 ttcaaaaatt tacatcgcga actattaatt ttgcagcaaa acaaggttaa atatttacat
      661 tttgatgtta tggatggaca ttttgttcca aatttatcat ttgggattcc aattttagaa
      721 gcacttaaac ctcattatat atttatttat gatgtacatt taatggttac taatcctgaa
      781 tttattgctc caaaatttgt ggaagctgga gcggatatcg ttacttttca ccttgaagca
      841 atgaaaactg gtaatgatgt tgttacacta attaaagaat tacgactgat gggtgtaaag
      901 gtaggtatta gtattaaacc tgatacacct gttgaagact tagcgccgat tttaaaagat
      961 atcgatttag tgcttgtgat gtcagttgaa ccaggctttg gtggacaaag tttcttacgt
     1021 agtgctttac ataaaattga atggcttgat gaagagcgca aaaagtataa atataattac
     1081 ttgatcgaag ttgatggcgg tatcaatgct gaaactgggg cactttgtgc tgaagcaggc
     1141 gctgatattc ttgtcgcggg ttcatactta tttggtaaag aagacattaa tgaaaggata
     1201 aagggattac gtaatggata agagtattgt tcatattgct tttttttcat cattatcact
     1261 gtttgtgatt actttaattt ttcaattatc actttatcgc actaaacaaa atcggaagtt
     1321 ttcgtttcgt aatgagctgc cgtttgaact tgtacaaggc gccgatatca aatttatcaa
     1381 ttatcactat gtattattgt ttcttgtgac gatcgctaat ttattatttg cctttaaata
     1441 tttagatcat atttataatt ggtacgaata tttattagtc ggttcactcg tattaagtgc
     1501 gataatgctc tatcttattt tctttattaa agtatttgaa attaaaaaac atattatcgt
     1561 tgttatctta caagctttat ctgttgtcac aagttatctt tcatttggtt tatttgcgca
     1621 cattagtcca tttggtaaac aaaatatcgt ctttggtatt tttggttact tatttgcgct
     1681 tattgggatg ttagtgttac ttaatccacg acttcgcaaa tggccgatta tggataaagt
     1741 tttacagcaa gatggaacgg tacttatttt gcgaccgcgc tattttatgc tagcgcttta
     1801 tgaatggggt tttattgccg cacagttttt attaatgatt gtaatgtacg cttatcttta
     1861 tgtttaagta gaaagaagaa gataataaaa aaaactcact ttgttgtgag ctttttttat
     1921 actttggcgg acttgttaag tgagcgatat gctcttgcag acatccggac ggtttggact
     1981 ttgccatcaa cttcaacttt atagttttgt aagtttacgc cccagcgccg tctagttgcg
     2041 cgaagagagt gactgcgttt attgccactt aagggtcctt tgcctgttac tggacaatgt
     2101 ctagccataa tttcctcccc attcaataaa tgctacatta agataacatt aatgttataa
     2161 atttgcaaat ataaaaagtt aaattttgaa aattattgca aatttgcaca tattgttaat
     2221 aaataccttt gttattaatg tgtattttag tccacattat gttatactct ctctatggat
     2281 ttaattaata agaatatcgg tgaaaaaata aaaagcagaa gattgcgttt aggctactca
     2341 caattaatgt tatcggaact tgctcatgta tcccgtgacc aaatttcacg gattgaaagc
     2401 gggaagataa attttacatt agcgacacta tttaaaattt gtcgggcgct tagaattaaa
     2461 cctcataaat tgcttaaaga tgctgaacaa gaagaatata ttaaaagtgg ggaccttcgt
     2521 ccttttgtta aatgggcggg tggtaaaacg caaatattag acgaacttat gcaaagaatt
     2581 cctaatgagt tcaataatta ctttgaacct tttgtggggg gtggcgcgtt ctttttaaaa
     2641 ctcgaacacc cgcgttccgt cattaacgat attaacagtg aactaatgac tgcttatagt
     2701 gttattaaag ataaagaaaa atttattgca ctgaagaaac tattactaca atatgaagaa
     2761 aaacataatg aggaatttta ctatgaggtt cgcgcgagag accgtgaccc ttcattttcg
     2821 actttaagtg atgttgaaat tgccgctagg tttatatact taaataagaa ttgttttaat
     2881 ggtttgtacc gagtgaattc caaaggttat tttaatgtgc catttaataa aagtgaaaaa
     2941 gtaaaaactt tcgatgaaga taatataaat aaactacata aatatctgtc attacctagt
     3001 attaaaatat gttgtgaaga ttacaagaat atgactaaca aagctaaaaa aggtgatttt
     3061 gtttactttg atccacctta tgacacgctt aaggatgata cattcactgc ctatgataaa
     3121 gaaggctttg gtcgtaatga acaaattgaa ctttcggaag aatttaaaaa attacataaa
     3181 aaaggtgtga aggttatgct ttcaaatcat aacacaccac tgattaatga actttataag
     3241 gagtttaata ttacgattat tcatgctcgt cgcgctatta attcacgtgg tagtgggcgc
     3301 ggtaaaattg aagaagtgat tattactaat tactaaaatg aatatcatta atgttggaaa
     3361 aagcgcactt aagataaacg atgatgattt tattttagtg caaggtaatg ctttgcgcgt
     3421 tttaaagaaa atagccccaa aatcagttga tgttattttt gctgacccac cttactttct
     3481 atcaaatgac ggtattacat gtgtaaacgg taaaatggtt tctgttaata aggctttgtg
     3541 ggataaaagt atcagcgcga cagataaatt aaatttcaac cgtaaatgga ttagatattg
     3601 tcgcgatgct ttaaaggatg atggaacaat ctggattagt ggcacaatgc ataatattta
     3661 ttcaatcggg gtagcacttg aattagaagg attttctatt attaacaaca ttacatggcg
     3721 caaacctaat ccaccaccga atattgcttg tcgcgctttt acccattcaa ccgagaccgt
     3781 gctttgggct cgtaaaatca ttaataaccg aaaaaagggc aaacatttct ttaattatgc
     3841 actaatgaaa gaaattaacg gcggtaagca aatgaaggac gtgtgggaat taaaaggtgc
     3901 gctaaaaaaa gaaaaagaac acggtaagca tcctacgcaa aaaccgtttg aactactaga
     3961 acgaattatt cttgcatcaa gtaatgaagg tgatattata cttgatccct ttgctggaag
     4021 tggcactact ggtattgccg caaaaagatt aaaaagacgt ttcattggga tagaaattga
     4081 aaaagaatat attggaatta ttaaaaaacg ttataaagca atacaataag tttgtattat
     4141 taaaa
//
LOCUS       DAOPCD010000069         4118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_17752_length_4118_cov_4.838051, whole genome shotgun sequence.
ACCESSION   DAOPCD010000069 DAOPCD010000000
VERSION     DAOPCD010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17752_length_4118_cov_4.838051"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLQ55_02525"
     CDS             <1..207
                     /locus_tag="PLQ55_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28839.1"
                     /translation="VIIILFVGQVLSYLARTIFIIQHSKKMFTRDLRDYKLSDLSAAP
                     AELQESGVNIPEVKPKEPLPPTNG"
     gene            complement(197..349)
                     /locus_tag="PLQ55_02530"
     CDS             complement(197..349)
                     /locus_tag="PLQ55_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28840.1"
                     /translation="MNTNKYGQDIPLTKEEIAIRGYKRRNLVLGIIIIILIIIIGIQI
                     GVMLTR"
     gene            complement(359..1726)
                     /gene="asnS"
                     /locus_tag="PLQ55_02535"
     CDS             complement(359..1726)
                     /gene="asnS"
                     /locus_tag="PLQ55_02535"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPK28841.1"
                     /translation="MLNVKALYTAYEKGELKAIPVKLQGWVKTNRNNGTIGFIEFNDG
                     SAFKGVQIVYEKNKIDNFAEVDKYRTGAALEVYGKVVLTPEMKQPFEVHADKVVLVGD
                     VAEDYPLQKKRHTPEFLREIAHLRPRTNTHTALFRLRSELSYALHKYLYDHEFVYVHT
                     PIITGNDAEGAGNVFNVVTDHKDPNFFFGKEASLTVSGQLHVESFALTFGKVYTFGPT
                     FRAEESNTTRHAAEFWMLEPELAFTDLNGNMDSIEEIIKYSVAYVLEKCSDEIDFFTK
                     FYDKTLKDRLEALVKSKVLRITYTKAVEILKEAQQKGVKFQFDNIFWGMDLASEHERY
                     LCEVYTNGPVFVTDYPKDIKAFYMRLNDDGKTVAACDLLVPGVGELVGGSQREDRYDV
                     LKAKMIEGGNLEDLEWYLDLRKYGGVPHSGYGIGFDRLLMYLTGVQNIRDVQPYARVF
                     GSIKY"
     gene            complement(1762..2775)
                     /gene="tsaD"
                     /locus_tag="PLQ55_02540"
     CDS             complement(1762..2775)
                     /gene="tsaD"
                     /locus_tag="PLQ55_02540"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPK28842.1"
                     /translation="MKERIILAIESSCDETAVAIMRGDKLLSNAVSTQISMHQKYGGV
                     MPELASRLHTENIVYTLDTALKDANIKLEEVDAFAVTRGPGLIGALHVGLQAAKTLAL
                     LYNKPLIPVHHLAAHIYASEFVKPLVFPLLAIVVSGGNTEFVVMKKHLDFTTIGETRD
                     DAIGECYDKIARVLNLGYPGGIPIDRLAKEGEATYKLPSPALTGYDFSFSGLKTHVIN
                     LVNNTKQRGEEINVPNLCASFQKVAIDAIIKKGLKAAQEYKVKQVVIAGGVSANSYLR
                     SEVLAKFPKIGVDPAIPPLWCCTDQAAMIAKLASYLYDEKVFADYDLGVDPNWEIEDF
                     REF"
     gene            complement(2772..3233)
                     /gene="rimI"
                     /locus_tag="PLQ55_02545"
     CDS             complement(2772..3233)
                     /gene="rimI"
                     /locus_tag="PLQ55_02545"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPK28843.1"
                     /translation="METKEKKLIIRPMTIDDVDAVDELAFAALPDPWPKYTYAQELKN
                     EFAYLFVLTYADVILGFSHFWITFDSATIVKFAVHPAAQGKGLGSLLMAHLIERVSML
                     EEVRAITLEVRTSNDKAMHLYLKYGFNVVTKKRKYYEDGEDAYYMIKVLKE"
     gene            complement(3238..3819)
                     /gene="tsaB"
                     /locus_tag="PLQ55_02550"
     CDS             complement(3238..3819)
                     /gene="tsaB"
                     /locus_tag="PLQ55_02550"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPK28844.1"
                     /translation="MITLLLDSSSHHLSVALAKGDKVLSFVEEKAFQTQSEIMVGRIN
                     KLLAQEKLTSDDIKRIVVASGPGSFTGVRIAVTIAKVWAYAKEVPLYAVSSLSVYRHE
                     TKPTICVLDARRERSFAGVYAQNNAILPDKIMQNSEILALAEKEGYLISGETKHLGIE
                     PAKFNRFENMLKSISEENRVKDIAAFKPLYLKG"
     gene            complement(3816..>4118)
                     /gene="tsaE"
                     /locus_tag="PLQ55_02555"
     CDS             complement(3816..>4118)
                     /gene="tsaE"
                     /locus_tag="PLQ55_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPK28845.1"
                     /translation="TEKVNSPTFNILKIYLKGDKPLFHIDAYRLEDDKKDIGLDEYIG
                     EAGITVIEWPAFIKHLLPEKALEINITHRDLTSRNITLKGEGHYADVVKVIAEEFK"
BASE COUNT         1234 a          856 c          647 g         1381 t
ORIGIN      
        1 gtgattatca ttttatttgt tggccaagta ctttcctact tagcgcgtac gatctttatt
       61 attcaacata gtaaaaagat gtttactcgc gatttacgtg attataaatt aagtgattta
      121 agtgccgctc ctgctgagtt acaagaaagc ggtgtgaata ttcctgaagt taaacctaaa
      181 gagcccttac caccgactaa cgggtaagca ttacaccaat ttgaattcca ataataatta
      241 ttaaaataat aataatgata cctaaaacga ggttccgtct tttataacca cggattgcta
      301 tttcttcttt tgttaagggg atatcttgtc cgtatttatt tgtattcact ttgcacctct
      361 aatatttaat tgagccaaat acccgagcgt aaggttgaac atcacgaata ttttgcactc
      421 cagttaagta catcaataag cggtcaaacc caataccgta tccagaatgg gggacaccac
      481 catatttacg taagtctaaa taccattcta agtcttcaag gttaccgcct tcaatcatct
      541 ttgcttttaa aacatcgtat ctatcttctc tttgtgaacc accaactagt tcaccaacac
      601 ctggcactaa taagtcacac gctgcgactg ttttaccatc atcatttagg cgcatataaa
      661 atgctttaat gtctttagga taatcagtaa caaacactgg accattcgta taaacttcac
      721 aaagataacg ttcatgttca cttgctaaat ccatacccca gaaaatattg tcaaattgga
      781 atttaacgcc tttttgctgg gcttctttta atatttcaac agctttagtg tatgtaatac
      841 gtaatacttt acttttcact aatgcttcta aacggtcttt taatgtctta tcataaaatt
      901 tcgtaaagaa atcaatttca tcggaacatt tttctaatac ataagcaaca gaatacttaa
      961 taatttcttc aattgaatcc atattaccat ttaaatcggt gaacgctagt tcgggttcaa
     1021 gcatccaaaa ctccgctgcg tgccgggtag tattactttc ttcagcacgg aatgttggtc
     1081 caaaggtata aactttacca aatgttaacg caaatgattc aacatggagt tggcctgaga
     1141 cagtaagtga agcttcttta ccaaaaaaga agtttggatc tttatgatca gtaacaacat
     1201 taaagacatt acccgctcct tcagcgtcgt ttccggtaat aattggtgta tgaacataga
     1261 caaattcatg gtcgtataag tatttatgaa gcgcatatga tagttcactc cggagtcgga
     1321 ataaagcagt gtgtgtattg gtccgcggtc ttaagtgcgc aatctctctt aaaaattcag
     1381 gagtatgccg tttcttttgt aacggataat cttctgcaac gtcaccaaca agaacaactt
     1441 tatcagcgtg tacttcaaag ggttgcttca tttctggagt taacacaact tttccgtaaa
     1501 cttcaagcgc cgctccagtg cgatatttat caacttcagc aaagttatca atcttatttt
     1561 tttcataaac tatttgtaca cccttaaagg cactaccatc attaaattca ataaaaccaa
     1621 tcgtgccatt attacgattt gttttgaccc atccttgtaa tttaacagga atcgctttaa
     1681 gttcgccttt ttcataggcg gtatatagtg cttttacatt taacatattg ttacctcttt
     1741 gacttcttta ttaattatat cttaaaactc tctaaaatct tcaatttccc aattaggatc
     1801 aacacctaaa tcataatccg caaaaacttt ttcatcatat aagtatgatg ctagcttagc
     1861 aatcatcgct gcttggtcag tacaacacca tagtggcggt attgcgggat caactccaat
     1921 tttcgggaat tttgctagta cttcgctccg taaatatgag tttgcgctca cgccaccagc
     1981 aattacaact tgtttgactt tatattcttg tgctgctttt aatccttttt taataatggc
     2041 atcaattgcg actttttgaa atgatgcaca taagttcggc acatttattt cttcgcctct
     2101 ttgtttagta ttattaacta aattaattac atgtgtcttt aacccggaaa aagaaaagtc
     2161 gtaaccagtt aaggcaggac ttggtaattt gtaagttgct tcaccttctt ttgctaaacg
     2221 gtcaattggg ataccgcccg gataacctaa atttaagaca cgcgctattt tgtcatagca
     2281 ctctccaatc gcgtcgtcac gcgtttcccc aattgttgta aagtctaaat gttttttcat
     2341 cacaacaaat tcagtgttac cgccacttac aacaatagca agcagtggaa acacaagcgg
     2401 tttaacaaat tcactagcgt aaatatgcgc tgctaagtgg tgaacaggga tcagcggttt
     2461 gttgtaaagg agcgctaatg ttttagcagc ttgtaaccca acgtggagcg ccccaataag
     2521 tccaggtccg cgagtcaccg caaaagcatc aacttcttca agcttaatat tcgcatcttt
     2581 tagggcagta tctaatgtat aaacaatgtt ttcggtgtga agtcttgagg ctaattcagg
     2641 cataactccg ccatactttt gatgcatcga aatttgtgta ctaacagcgt tacttaaaag
     2701 tttgtcacca cgcataatag caacagcggt ttcatcacaa ctagattcaa ttgctaatat
     2761 tattcgttct ttcattcttt tagtaccttt atcatataat aagcatcttc accgtcttca
     2821 taatacttcc gtttcttagt cacaacatta aatccgtatt ttaaataaag atgcattgct
     2881 ttatcgttac tcgttcttac ttctaatgta atagctctta cttcttcaag cattgacact
     2941 ctttcaatga gatgcgccat tagtaaactt cctagtccct tgccttgcgc cgcgggatgc
     3001 acagcaaatt taacaatggt ggcactatca aacgtaatcc aaaaatggga gaatccaaga
     3061 atcacatcag cgtaagttaa gacaaataga tatgcaaatt cattttttaa ttcttgggca
     3121 taagtatatt taggccacgg atcagggagt gccgcaaacg ctagttcatc aacagcgtca
     3181 acatcgtcta tagtcattgg acgaataata agtttctttt cttttgtttc catcttatta
     3241 tccctttaaa taaagcggct taaacgctgc tatgtcctta actctatttt cttcactaat
     3301 gcttttaagc atattttcaa aacgattaaa ctttgcgggt tcaattccta aatgttttgt
     3361 ttcgcctgaa attaagtaac cttccttttc cgctagtgct aatatttcgc tgttttgcat
     3421 tattttgtcc ggcaaaatgg cattattttg cgcgtaaacc ccagcaaaag aacgctctct
     3481 tcttgcatca agtacacaaa tggtcggttt tgtttcatgg cgataaacac ttaaactaga
     3541 aacagcataa agtgggacct ctttagcgta cgcccatact ttggcaattg ttacagcaat
     3601 tcttacccca gtaaaagagc ctggaccact tgcgaccacg attcttttaa tgtcatcact
     3661 tgttaatttt tcctgcgcta atagtttatt aattcttcca accattattt cactttgggt
     3721 ttgaaaagcc ttttcttcaa caaaacttaa aactttgtcg ccctttgcaa gcgcaacact
     3781 taaatgatga ctcgaactat ctaaaagtaa tgtaatcatt tgaactcctc agcgataact
     3841 tttaccacgt ccgcataatg accttcccct tttaatgtaa tgtttcttga agttaaatcg
     3901 cgatgcgtaa tattaatttc aagtgccttt tcgggtaaaa gatgcttaat aaatgccggc
     3961 cactcaataa cagtaatacc tgcttcacca atatattcat ctagtccaat atctttttta
     4021 tcatcttcta aacgataagc atcaatatga aataatggtt tatcaccttt taaataaatc
     4081 ttaagaatat taaaggttgg actattaact ttttctgt
//
LOCUS       DAOPCD010000070         4118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_17755_length_4118_cov_3.948560, whole genome shotgun sequence.
ACCESSION   DAOPCD010000070 DAOPCD010000000
VERSION     DAOPCD010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17755_length_4118_cov_3.948560"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1832
                     /locus_tag="PLQ55_02560"
     CDS             117..1832
                     /locus_tag="PLQ55_02560"
                     /inference="COORDINATES: protein motif:HMM:NF017926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005940 - septin ring [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HPK28846.1"
                     /translation="MKVVTLLNTLDIVFIVASIILVLAVFGFILSKFAFRGTKNKKAL
                     KSLSIKYSYVRSLLLGENIQYIKRIEAISDINLLMRPAYEEFFTRFEQLRNVDDRKAS
                     RSIQILNEALAAGNKEFKKAFNEHRPIILDYERKVTALSKDLTNMIKPESEIREESVY
                     DKEKLRIIKNIYRDHKDELEICVPSFEKIFTQIELLFKEYDNALNGAYYDEARSYLEK
                     IRTFNDQLERILQILPLLVVKATVLIPEKIATLKKSYEEATAYNVPLHHLRVSAEIES
                     FNEQLEVIKEQLIRFDTRNVEQTLNQIADTIDELINSFNVEIEKQREFTAEYDKTYLH
                     VNELEQRYIRLVNRMPQVKEYYLINPEYEKKLEEANTNVNTMMMVKRSLDAFLHSATK
                     QPYSVLVEKVQQLQAESMKVEVLVREFQTYIESLRVIVEDGFAICKLLFIRFKELEVT
                     LRKLNVPAYIQNFEQDFDSGYAMISDISTIIRERPIDVSQVEVLTNELRALATRLEDV
                     ITTDVKYAHSAEEVIVSLNAFRPQFSELHSKLRDEEQRFFNGLFKHTYENSIEQLKKY
                     QNRKQ"
     gene            1829..2962
                     /locus_tag="PLQ55_02565"
     CDS             1829..2962
                     /locus_tag="PLQ55_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPK28847.1"
                     /translation="MIYFDNASTTKIHPEVLRTYIEILEKYYGNPSGMHTFAREIARL
                     QDKSREEILRMFKFRDGKVIFTSGATEANNIALIGLYEAYKNRGNEIIISDYEHPSVR
                     AACEYLEKERGAIIHKVPIRFKGGFDFDVFKSLLNDKTVLVSVMAVNNEVGDVIDLGE
                     IRACLKKYPKVIFHTDVTQALGKLDFHPNAADAYSFSAHKIGGLKGSGALLLRKNLKP
                     KPLVYGGSQEEGIRPGTHNAPANIVLAKTVRIAEGNMDKFYDHSYRLALKLHNFLATH
                     KDLFIINSVGGSNPYIVNFSLKTIKASIVLNALESRDIYVGTTSSCSAKLHAMSKSVY
                     AISQDEERASNSIRVSFGMENTLDEVEIFCNTLTEIIKEYGRE"
     gene            2949..>4118
                     /gene="thiI"
                     /locus_tag="PLQ55_02570"
     CDS             2949..>4118
                     /gene="thiI"
                     /locus_tag="PLQ55_02570"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HPK28848.1"
                     /translation="MAVSKMLMIHFGELYTKGKNRRDFIRQLTNNIKEVVANFKVQIE
                     TRHDHIYIKNIKEKDVAEITKRLQNVSGIHAISEVIEFPLDLEKVKQFALELILELKP
                     KTFKVITKRKDKLFPLTSDEINRSVASHILKNAEIKVDVHNPELPLRLEIDKSRILVV
                     GKRYDGLGGYPLGAAGKGLVLLSGGIDSPVAAYLMIRRGIKIAAIHFAAPPYTSEEVL
                     KKIEALTAKLNNYQPHIRLYVVPFTKLQLAIYEHATSGYAITLMRRTMLRISTVIAKR
                     HGYKVLVTGDSLGQVSSQTLESLVVINEVTNYPIIRPLATLDKNEIIKIAKDIETYPI
                     SILPYEDCCTIFPVTNSVTSPKLEDAVLFESKFDYESLISEAIRDTKKVDIRLDDG"
BASE COUNT         1445 a          664 c          745 g         1264 t
ORIGIN      
        1 aaaatcatta aaacacttaa tcttgttaat tttaattatt catctatcca aatatatgtt
       61 aacgtatgaa aacattttca aacattgtat aatatacaat aagtgaggtg cgacttatga
      121 aagtagtaac attattaaac acactcgata ttgtcttcat tgtagcaagt atcattcttg
      181 tcctagcggt tttcggattt attttaagca aatttgcctt ccgtggcaca aaaaataaaa
      241 aggcattaaa atctttatca attaaatatt catatgtgcg cagcctactc cttggtgaaa
      301 atattcaata tattaaacgt atcgaagcaa ttagtgatat taatttacta atgcgtccag
      361 cttatgaaga attctttacg cgctttgaac aattacgcaa tgtcgatgac cgtaaagcaa
      421 gtcgtagtat tcaaatatta aatgaagcac tcgcggcagg taataaagaa tttaaaaaag
      481 cttttaatga acatcgcccc atcattcttg attatgaacg taaagtcaca gcgttaagta
      541 aagatttaac gaatatgatt aaacccgaat cagaaattag ggaagagagt gtttacgata
      601 aagagaaatt acggattatt aaaaatattt atcgtgatca taaagatgaa ctggaaattt
      661 gtgtaccaag ctttgaaaaa atatttacac agattgaatt actctttaaa gaatatgata
      721 atgctcttaa tggtgcttat tatgacgaag ctcgtagtta tttagaaaag attcggacat
      781 ttaatgatca acttgaacgc atcttacaaa ttttaccgct tttagttgtg aaggccacag
      841 tgttaatccc tgaaaaaata gcgacattaa agaagagtta tgaagaagca acggcttata
      901 atgttccact tcaccattta cgtgtcagtg ctgaaattga aagctttaat gaacagctcg
      961 aagtgattaa agaacaactt attcgttttg atacacgcaa tgtcgaacaa acacttaatc
     1021 aaattgccga tacaattgat gaattaataa atagtttcaa tgttgaaatt gaaaaacaac
     1081 gcgaatttac ggcagaatac gataaaacat atttacatgt taatgaatta gaacaacgct
     1141 atattcggct tgttaatcga atgccgcaag tgaaagagta ttatttaatt aatcctgaat
     1201 atgaaaagaa attagaagaa gctaacacta atgttaatac aatgatgatg gttaagcgta
     1261 gcttagatgc ttttctccat agtgcaacca aacaacccta tagtgtgcta gttgagaaag
     1321 tccaacaact gcaagctgag agtatgaaag ttgaagtgct cgttagagaa tttcaaacat
     1381 atattgaatc actccgtgta attgttgaag atggctttgc gatttgtaaa cttttattta
     1441 ttcgctttaa agaattagaa gttactttgc gtaagcttaa tgtcccagcg tatattcaaa
     1501 actttgaaca agattttgat agtggttacg caatgattag tgatatcagc acgattatcc
     1561 gtgaacgacc aattgatgtg agccaagtcg aagttttaac aaatgaatta cgtgcgcttg
     1621 ctacacgctt agaagatgtg attacaactg atgtaaagta cgctcattca gcggaagaag
     1681 ttatcgttag tcttaacgct ttccgtccac aatttagtga attacattca aaactccgtg
     1741 atgaagaaca acgcttcttt aatgggcttt ttaaacatac ttacgaaaat tcaatcgaac
     1801 aattaaagaa ataccaaaac cgtaaacaat gatttatttt gataacgcta gtacaacaaa
     1861 aattcatcct gaagttttaa gaacatacat tgagattctt gaaaaatatt acggcaatcc
     1921 aagtgggatg cacacttttg cgcgtgaaat agcaagatta caagataaat cacgcgaaga
     1981 aattttaaga atgtttaagt ttcgtgatgg taaagttatt ttcactagtg gcgctactga
     2041 agcaaataat attgcgctta ttggtttata tgaagcttat aaaaatcgtg gtaacgaaat
     2101 aattattagt gattacgaac accctagtgt gcgtgccgca tgtgaatatt tagagaaaga
     2161 acgtggggca atcattcata aagtgccgat tcgctttaaa ggtggttttg attttgacgt
     2221 ttttaaatcg ctattaaatg ataaaacggt gcttgtctcc gttatggctg ttaataatga
     2281 ggttggtgat gtcattgatt taggggagat tagagcatgt ctaaaaaaat atcctaaagt
     2341 tatatttcac actgatgtca ctcaagcctt aggcaaactt gactttcatc ccaacgctgc
     2401 agatgcttat tcattttccg ctcataaaat cggcggatta aaaggaagtg gtgcactact
     2461 tttacggaaa aacttaaagc caaagccact agtgtatggc ggcagtcaag aagaagggat
     2521 aaggccggga acacataatg ctccggcgaa tattgtatta gcaaaaacag tgcgtatcgc
     2581 tgaaggtaat atggataaat tttatgatca ttcttatcgc ttagcactta aattacataa
     2641 ttttcttgct actcataaag atttatttat tattaattcg gtcggcggaa gtaatccgta
     2701 tattgttaac ttttcactaa agacaattaa agcttcaatc gtgctcaacg ccttggaaag
     2761 tcgcgatatt tatgttggca cgactagctc ttgtagcgct aaattacatg caatgagtaa
     2821 gtcagtatat gccatttcgc aagatgaaga acgcgctagt aactcaatcc gagtaagttt
     2881 tggaatggaa aatacgcttg atgaagttga gatattttgt aacacattaa ccgaaattat
     2941 aaaggagtat ggccgtgagt aaaatgttaa tgattcattt tggtgaactt tatacgaaag
     3001 gcaaaaaccg tcgtgatttt attcgtcaat tgactaataa cattaaagaa gttgtcgcta
     3061 atttcaaagt acaaattgaa acaaggcatg accatattta tattaaaaac atcaaagaaa
     3121 aagatgttgc tgaaattaca aaacgcctgc aaaatgtcag cggaatccat gcaattagtg
     3181 aagtaataga attcccgtta gacctagaaa aagttaaaca atttgcgctt gaactcatcc
     3241 ttgaactaaa gccaaaaaca tttaaagtta ttactaaacg taaagataaa ctttttccat
     3301 taacgagcga cgaaattaat cgtagtgtcg caagtcatat tttaaaaaat gctgaaatca
     3361 aagttgatgt ccataatcct gaattaccac tccgccttga aattgataag agtcgtattc
     3421 ttgttgttgg taaacgttat gacggcttag gcgggtatcc gcttggtgct gctggaaaag
     3481 gtttagtgct ccttagtggc ggaattgatt caccagttgc ggcttattta atgattagac
     3541 gcggaattaa aattgcagca atccactttg cggctccacc ttatacaagt gaagaagtat
     3601 taaagaaaat tgaagcacta accgcaaaac ttaataacta tcaaccgcat attagattat
     3661 atgtcgtacc atttactaaa ctccaattag cgatttatga acacgctaca agtggttacg
     3721 ctattacctt aatgcgacgc acgatgcttc gcattagtac tgtcattgct aagcgtcacg
     3781 gttataaagt actagtgact ggtgattcac ttggtcaagt ttcaagtcaa acacttgaat
     3841 cactcgtcgt tattaatgaa gtcactaatt atccaattat tcgcccatta gcgacgctcg
     3901 ataaaaatga aattataaaa attgctaaag acattgaaac ttatccaatt tcaattttac
     3961 cttatgaaga ttgctgtaca atattcccag ttacaaactc agtaactagt cctaaactag
     4021 aagatgcagt gctttttgaa agtaagtttg attatgaatc tctcatcagt gaagcaattc
     4081 gtgatacgaa aaaagttgac attagattag atgatgga
//
LOCUS       DAOPCD010000071         4112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_17786_length_4112_cov_2.825980, whole genome shotgun sequence.
ACCESSION   DAOPCD010000071 DAOPCD010000000
VERSION     DAOPCD010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17786_length_4112_cov_2.825980"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..716
                     /gene="lspA"
                     /locus_tag="PLQ55_02575"
     CDS             153..716
                     /gene="lspA"
                     /locus_tag="PLQ55_02575"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPK28849.1"
                     /translation="MNKVKQYLKSFFYSYAWLAVLLLIIDQLTKVWALKAKLDITLIP
                     NFLHLTYVRNTGAAWSMFDGNMLVLALISFVVGTLLIVARIKYRTKLDTFKKILFAVV
                     LAGTWGNFIDRAFYKLLTGAEGVVDYIHFQFGNYHFPVFNVADICITLGILSYVVLLL
                     VEEFKALKAPKTEEKVKDDTNEEKDGE"
     gene            706..1620
                     /locus_tag="PLQ55_02580"
     CDS             706..1620
                     /locus_tag="PLQ55_02580"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPK28850.1"
                     /translation="MENKLLKVDFEHSGKRADVVLSSLLSITRAQVQKMIKDGDIKLN
                     GTSVKASYRIEGGEEFSVTIREKEAPNLTPYEIELDVRYEDEYLLVVNKPAGLVVHPS
                     FGHKKETLVHALLARGDKYSDVNGVFRPGIVHRLDKETSGLLIVCKNNEVHNKIAKLL
                     KKREITRRYTALVRGKVREEAGVIKTYLTRSKDNFKKMANTPATGKLAISHFKVKERF
                     KGYMLLEVELETGRTHQIRAHLEFINTPIVGDALYGLKKDPLYSKGQLLHASYLAFTH
                     PYTGELVEVSAELPDYFTKVLRRLQKDL"
     gene            complement(1583..2515)
                     /locus_tag="PLQ55_02585"
     CDS             complement(1583..2515)
                     /locus_tag="PLQ55_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HPK28851.1"
                     /translation="MHNYELRIPREDLGIVIEQTKLPSVSNKHSYMIIYYEKGNIKFS
                     IYDNTKNEPYKARFVGARLPKYLEPYLKEKDIKTPKSKSVTTKIVDKKITTFPHIGSD
                     EVGFGDFFGPLVVVAAYLDEELKALVESLNVKDSKALDDTKIVLLGNLLKDKIPHTKN
                     IVHNPKYNELIAQGYNMNQMKAMLHQNVLTTLAKRQKYDGKLYLDAFTSDKHYENYTA
                     LMSKAKVIQLKDGESNSLAIATASILARFYFLREIEVLNIRYNTKIPLGAGNAVDKFA
                     QEFLVKFGKNNLLSVVKQNFRNFKDLFADDGGLL"
     gene            2586..>4112
                     /locus_tag="PLQ55_02590"
     CDS             2586..>4112
                     /locus_tag="PLQ55_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28852.1"
                     /translation="MDIFTLLEFNEIVSKVKDLTKTELGGEYASLISPFESEEEATYA
                     LNLVSEAESILTRFGALPLAPSHKLAPLLEEARRGNILTPHDFYLISADAMNIVKINY
                     YLKDKLDNAPLLKAMLKREKDLAVLANEINKVITPNLLVKDNASHELARIRKNIALSE
                     DRLNKAAQGLISKYGNFLAENLITKRDGHFVIPVKTAYKNKVDGIIYDISNSGQTIFV
                     EPSEIAALNSTLISYQNDEIIEVNRILRALTLETLKYEDEIINNNILLAELDLIFAKA
                     TYGQSINGFVPVFQQTKGLQFKKARHPLIDKNLVVANTFNLKDDKFIIVISGPNAGGK
                     TVALKTMGLLVLMAKSGLMLPVSEEPKLFFYKNVYASIGDEQSLSQNLSTFSSHVKRI
                     GEIMDVATSDDLVLLDELATGTSPEEGEALALAIATYLLNNSISSVISSHYSRLKEFA
                     YSRSGIINASMAFDEKKLTPLYEYHEEIPGRSYGLVVAKRFGIKDEVIKDAESFLSDG
                     V"
BASE COUNT         1328 a          680 c          789 g         1315 t
ORIGIN      
        1 ctttattgta agtgaagtta agttttgtaa tacttataaa gtagaaaagc acactggtca
       61 caaatgtccg cggtgctgga attatcatga tgaattagta agcgttgctg aagaacaagt
      121 ctgtgaacga tgcgcaaagg tattaaacac atatgaataa agtaaaacag tacttaaaat
      181 cattctttta ttcctatgct tggttagccg tgctcctttt aatcattgac caattaacaa
      241 aagtatgggc actaaaggcg aagttagaca tcacgcttat tcctaatttc ttacatttaa
      301 catatgtgcg aaataccggc gcagcgtgga gtatgtttga tggtaacatg ctcgtcttag
      361 cactgattag ttttgttgtc ggtacgctgc ttattgtagc gcgcattaag tatcgcacaa
      421 agttagatac atttaagaaa atactttttg cggttgtcct tgccggaact tggggtaatt
      481 ttattgaccg cgctttctat aaacttttaa caggtgcaga aggtgttgtt gattatattc
      541 atttccagtt tggtaattat catttccctg tgtttaatgt cgctgatatt tgtattactt
      601 tagggatctt aagttatgtc gttttattat tagtggaaga gtttaaagcg ctaaaagctc
      661 caaaaactga agaaaaagtt aaagacgaca ctaatgagga aaaagatgga gaataaactt
      721 ttaaaagttg attttgagca tagtggtaag cgcgctgatg tagtgctttc ttctttatta
      781 agtattacgc gcgcgcaagt gcagaaaatg attaaagacg gggacattaa acttaacggc
      841 accagcgtta aagcaagtta ccgtattgaa ggcggcgaag agtttagtgt tactattcgc
      901 gaaaaagaag caccaaactt aacaccctac gaaattgaac ttgatgtgcg ttatgaagac
      961 gaatatttgc tcgttgtcaa taaacctgcc ggattagtcg tgcatcctag ttttggtcat
     1021 aaaaaagaaa ctttagtgca tgccctttta gcgcgcggtg acaaatatag tgatgttaac
     1081 ggtgtttttc gtcctggtat tgttcatcgc cttgataaag aaacaagcgg tcttcttatc
     1141 gtatgtaaaa ataatgaagt gcataataaa attgctaaac tcttaaagaa gcgtgaaatc
     1201 actagacgat acacagcgct tgtgcgcggt aaagtgcgtg aagaagcggg tgtaataaag
     1261 acatacttaa cacgcagtaa agacaatttt aaaaagatgg ctaatactcc cgcaactggt
     1321 aaactagcga ttagtcactt taaagttaaa gaacgcttta agggctatat gcttttagaa
     1381 gtggaattag aaacaggccg tactcatcaa attagagcgc acttagaatt tattaatacg
     1441 ccaattgttg gtgacgcgct ttacggttta aaaaaagacc cgctatatag taagggtcaa
     1501 ttattacacg ctagttattt agcgtttacg catccgtata ccggagaatt agtagaagta
     1561 agtgcagagc ttccagatta ttttacaaaa gtcctccgtc gtctgcaaaa agatctttaa
     1621 aattacggaa gttttgtttc acgacactta ataaattatt tttaccaaat ttaaccaaaa
     1681 attcttgcgc aaatttatca acagcatttc ccgctccaag cgggattttt gtattataac
     1741 gaatgtttaa tacttcaatt tcacgaagga agtagaagcg cgctaatata ctagcagtag
     1801 caatcgctaa tgaattactt tctccgtctt ttagttggat gacttttgct ttactcatca
     1861 gtgccgtata gttttcgtaa tgcttatcac tcgtaaaggc gtcaagatac agtttaccat
     1921 cgtatttttg ccgtttagca agtgtcgtta acacattttg atggagcatt gctttcattt
     1981 ggttcatatt gtaaccttgt gcgataagtt cattatattt cgggttatgc acaatatttt
     2041 tagtgtgggg gattttatct tttagtaaat taccaagtaa gacaattttt gtatcatcaa
     2101 gcgcttttga atctttcaca ttaagacttt ccactagggc ttttaattct tcatctagat
     2161 aagcggcaac aacgacaagt ggaccaaaga agtcaccaaa cccgacttcg tcactgccaa
     2221 tatgcgggaa agttgttatc tttttatcga ctattttagt cgtaacgctt ttacttttag
     2281 gcgttttaat atctttttct tttaagtacg gttcaagata tttaggtaaa cgcgcaccaa
     2341 caaaacgcgc tttatatggt tcatttttcg tattatcata aattgaaaac ttaatgtttc
     2401 ctttttcata ataaataatc atatatgaat gtttatttga aacactaggt aattttgttt
     2461 gttcaatcac aatacctaaa tcttcacgcg gaattcgtaa ttcatagtta tgcatgagta
     2521 tattttaaca taaacgacaa tcttgttcgt tattagaaaa taatcattaa tgataaaata
     2581 caaatatgga tatatttaca cttttagaat ttaatgaaat cgttagtaaa gttaaagatt
     2641 taacaaagac agaattaggt ggtgagtacg ctagtcttat tagtcctttt gagagtgaag
     2701 aagaagcaac ttacgcactt aatttagtaa gtgaagcaga gtcaatttta acacgctttg
     2761 gtgcgttgcc actcgctcca agtcataaac ttgccccgct tcttgaagaa gcacggcgcg
     2821 gtaatatttt aacaccacac gacttttatt taataagtgc ggacgctatg aatattgtta
     2881 agattaatta ttacttaaaa gataagctag ataatgctcc cttattaaaa gcaatgctta
     2941 aacgcgaaaa agatttagcg gtgctcgcta atgaaattaa taaagtaatt acgcctaatc
     3001 ttttagtaaa agataatgcg agccacgaat tagcgcgcat tagaaaaaac attgccttga
     3061 gtgaagatcg ccttaataaa gcagcgcaag gtttaattag taagtacgga aacttcttag
     3121 cggaaaatct tattactaag cgcgatggtc actttgtgat tcccgttaaa accgcctata
     3181 aaaataaggt tgacggcatt atttatgata ttagtaattc cggccaaaca atatttgttg
     3241 aacctagtga aatcgccgca ctaaatagta ctttaattag ttatcaaaat gatgaaataa
     3301 ttgaagttaa tcgcatttta agagcattaa ctttagaaac tttaaaatat gaagacgaaa
     3361 ttataaataa taacatctta cttgcggaac ttgatttaat cttcgctaaa gcgacttatg
     3421 gtcaaagtat taatggcttt gtccccgttt ttcaacaaac aaaagggtta caatttaaaa
     3481 aagcccggca cccgcttatt gataagaatc ttgtcgttgc taatactttt aatttaaaag
     3541 atgataaatt catcattgtt ataagtgggc ctaacgctgg tggtaaaaca gtagcgctta
     3601 aaacaatggg tcttttagtg ctgatggcaa aaagcgggct catgttaccc gtaagcgaag
     3661 aacctaaatt atttttttat aagaatgtgt acgctagtat tggcgacgaa caaagtttaa
     3721 gtcaaaatct ttcgacattc agtagtcatg tgaagcgtat tggggaaata atggatgttg
     3781 cgactagtga cgaccttgta ttacttgatg aactcgcgac tggcactagt cctgaagaag
     3841 gggaagcact tgccttagca atcgctactt atttattaaa taacagtatt agcagtgtaa
     3901 ttagcagtca ctatagtaga cttaaagaat ttgcctactc ccgcagtggc atcattaatg
     3961 cttcaatggc gtttgatgaa aagaaattaa caccgcttta tgaatatcat gaagaaatac
     4021 caggacgctc ctacggttta gttgtcgcta agcgctttgg gattaaagac gaagttatta
     4081 aagacgcgga gtccttttta agtgacggcg tt
//
LOCUS       DAOPCD010000072         4046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_18130_length_4046_cov_2.476071, whole genome shotgun sequence.
ACCESSION   DAOPCD010000072 DAOPCD010000000
VERSION     DAOPCD010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18130_length_4046_cov_2.476071"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            900..>4046
                     /gene="cas9"
                     /locus_tag="PLQ55_02595"
     CDS             900..>4046
                     /gene="cas9"
                     /locus_tag="PLQ55_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HPK28853.1"
                     /translation="MKKQNNYYIGLDLGTNSVGWAVTNEKYELLHLKRKPAIGVNTFE
                     KAKTAEQRRNYRSTRRRLARRKQRVLLLQTFFAEEVAKKDPTFFLRLNNSPYFKEDKH
                     HSLQDTKFSLFNDKDFTDKDFHKKYKTIYHLQNDIISQPDKKFDIRLVYLAVHHLIKY
                     RGNFIKQDENINVAEFTNSLVTDAFQELAHYFEDTDLIAAQNFFVNENVRKYEAIVSD
                     KTNGGLKRATDKIFEQFEIKDKTLKTLLSLALGKVTRLNQIFSDEIVDDEQGKITIDF
                     KSISYEEKYNDIKTVIGEVNIVLIDELKRLYDYFVISRLMDGKTLVAEAMVEKYEKHR
                     SDLALLKKIFREHASKEDYRKVFTTLYTTDDKGKKKVITDVNYPRYIGKKSPYVIVKK
                     GDYADFKKFLNKMFKDLGLLSLNDVIKVQEGLDDETFLPLINSKDNATIPYQFNEYQL
                     RLILENQSKYYKFLNEKVDESSLTTLEKIMSIISFKIPYYIGPLVAEDNHPFAWVKRK
                     SFEKVTPWNFSDVIYEATSASRFIERMTNKCTYIPTEDVLPREAILYQEYLALDVLNN
                     LKINGEAIDNYAKQTLLTALYSNKTPLTKKRIATILAKNKNDVVAVSGIDDDVKLAFT
                     SLHKFKAILGENQDLTIEDMEKIIFYRTIFTEQKMFNKKVDELLGHRLSEELCKKIKQ
                     LKFKEWSRFSNAFLTGFSKTLNKPVLFDEKTGEVISIIDIMRNNVLSLQQVIHHEKYG
                     VLEALNKYNESMKTNENAFEDVYNLYLSPSVKRPVIKAIHLVDEIIKANDNKLPKKIF
                     LEVTRHDDFARKGTKTISRKKQLDDLYKIAVKESKDLKDLMDELNKKTDADLRSRKLY
                     LYFLQHGKCLYTGNPIALENLVMGDYDIEHIIPQSLITDDSLNNICLVESNINKAKGD
                     VYPLPRTIVNVEKMKPFWERLMKRGFMSESKYQRLIRMSELTEEERFSFINRQLVETS
                     QANKAVAKILEDRYGKGSVVYSKAQRVSDFRHKFKLLKQRDLNKAHHAHDAYLNIVVG
                     NYNDAQTRLAYINKQTGKN"
BASE COUNT         1576 a          509 c          656 g         1305 t
ORIGIN      
        1 tttttactat gctcttatca tttaacttga tttattaata gttaaaacct cctgcatata
       61 tattagtttg cattaagtac aggtttttgg tatcagttca ccgatgccaa acctctctac
      121 cgtaagctgg taccgagttt tatttttgtt ggactttctg atgtataaat tgatgctcga
      181 aaaaggggcc gctgtcattt aatttagtca cccaggcaaa attaaacaca aattaacccc
      241 tgaaggtcgt gctttataaa aatacaccat cactaaatca aattatcata gggtattcac
      301 tcgttaatta ttagggtatt caaagtatca aactaaacgt gtagttccat gataaaagaa
      361 aaggcaacaa aaaagttgct ttttatttta ttcataaaat gattacagct cactaacttt
      421 ttaaaaaaag agaaaaacag caaaaataaa atccgctatt taagattata aatggtgtgc
      481 tactcgaaaa acttttgaat tttaagaatt tttcgagtag agcgattata agatggttat
      541 tgattaaagt atatgattat tttttataac gaattaatta aaacttcatg tctgaccttt
      601 ttaatactgt aatgtgttat tgtagtatta gtattaggtg ttctatgaca aattttattg
      661 gcaaggattt tattcttgct ttagttaata ttacaaaacc ataattttaa tgattagata
      721 tgtgatatta acggagatta ttgtttattt atgagtgttc ataaattata attaaaacaa
      781 taaataaaaa actcgccgaa gcgagatgcc ttctacaggc tagacattaa gcaagtgctt
      841 tatttgaact tggtaatgtt atctgttttc attttaacta tagaggaggg taatgtcaaa
      901 tgaaaaaaca aaacaattat tacatcggct tagatttagg tacaaatagc gttggttggg
      961 ctgttacaaa tgaaaagtat gaattattac atttaaaaag aaagcctgct attggcgtga
     1021 atacatttga aaaagctaag accgcagaac aacggcgtaa ttatcgttca acaagaagac
     1081 gtttggcacg acgtaaacaa agagtgttat tactgcaaac attttttgca gaagaagtag
     1141 caaagaaaga tccaactttt tttctaagac taaataatag tccttacttt aaagaagata
     1201 aacatcactc attacaagac actaagttct ctttatttaa tgataaagat tttactgata
     1261 aagattttca taaaaaatat aaaacaatat atcacttaca aaatgatatt ataagtcagc
     1321 ctgataagaa atttgatatt agattagttt atttagcggt gcatcattta attaaatatc
     1381 gcggtaattt cattaaacaa gatgaaaaca ttaatgttgc ggaatttaca aattcattag
     1441 taactgatgc atttcaagaa ttagcgcact attttgaaga cacagattta attgctgctc
     1501 aaaacttttt tgttaatgaa aatgttagaa aatatgaagc aatagttagc gataaaacaa
     1561 atggtggttt aaaacgcgcg actgataaaa tatttgaaca atttgaaatc aaagataaaa
     1621 cactaaaaac attattaagt ttagcgttag gcaaggtaac aagacttaat caaattttta
     1681 gtgatgaaat agttgatgat gagcaaggta aaataactat tgattttaaa tcaatatctt
     1741 atgaagaaaa atataatgat attaaaacag ttattggtga ggttaatatt gttttaattg
     1801 atgaactaaa acgactttat gactactttg ttatttcgcg tcttatggat gggaaaacac
     1861 ttgtagcgga agcgatggtg gaaaaatatg aaaaacatcg cagcgattta gcattactta
     1921 agaaaatatt tagagaacac gctagtaaag aagattatcg caaagtattt actactcttt
     1981 acactactga tgataaggga aagaaaaaag taataacaga tgtaaattat cctcgctata
     2041 ttggtaaaaa aagtccgtat gttattgtta aaaaaggtga ttatgctgat tttaaaaagt
     2101 ttcttaataa aatgtttaaa gatctcggtc tactttcatt aaatgatgtt attaaagtgc
     2161 aagaaggact agatgatgaa acattcttac cattaattaa ttcaaaagat aatgcaacga
     2221 ttccgtatca atttaatgaa tatcagttgc gattaatttt agaaaatcaa agcaaatatt
     2281 ataaattttt aaatgagaaa gtagatgaaa gttccttaac tactttagaa aaaataatgt
     2341 caattataag ttttaaaatc ccttattaca ttggaccatt agttgcagaa gataatcatc
     2401 catttgcatg ggtaaaaaga aaatcatttg aaaaagtaac accatggaat tttagtgatg
     2461 taatttatga ggcaacgagt gcttcaaggt ttattgaaag aatgactaat aaatgtacat
     2521 atatcccaac agaagatgta ctaccaagag aagcaatact atatcaagaa tatttagcgt
     2581 tagatgtact taataattta aaaattaatg gtgaagcaat tgataattac gctaaacaaa
     2641 cattgctaac tgctctttat agtaataaaa cgccacttac aaaaaagaga atagcaacca
     2701 ttcttgctaa aaataaaaat gatgttgtgg cagtttctgg gattgatgac gatgttaaac
     2761 ttgcttttac ttcacttcac aaatttaaag caattttagg agaaaaccaa gatttaacaa
     2821 ttgaagatat ggagaaaatt attttctatc gcactatctt cactgaacaa aagatgttca
     2881 ataaaaaagt ggatgaatta ttagggcata gattatctga agaactttgc aaaaaaataa
     2941 aacaactaaa atttaaagaa tggagcaggt tttcaaatgc gtttttgaca ggctttagta
     3001 aaacgctaaa taaaccagta ttatttgatg aaaaaacagg tgaagttatt tcaattattg
     3061 atattatgcg aaataatgta cttagtttac aacaagttat tcatcatgaa aaatatgggg
     3121 ttttagaagc tcttaataaa tataatgaat caatgaaaac taacgaaaat gcttttgaag
     3181 atgtatataa tttatatctt tcaccaagtg ttaaacgccc tgtgattaaa gcgattcatc
     3241 ttgttgatga aattattaaa gcaaatgaca ataagttacc taaaaaaata tttttagaag
     3301 taacacgcca tgatgatttt gctagaaaag gcactaaaac tatttcacgg aaaaaacagc
     3361 tagatgattt atataaaata gcagttaaag aatcaaaaga tctaaaagat ttgatggatg
     3421 aattaaataa aaaaactgat gctgatttac gttcacgaaa attatatttg tattttctcc
     3481 aacacggaaa atgtttgtac accggaaatc ctattgcgtt agaaaatttg gtaatgggag
     3541 attacgatat tgagcatatt attccgcaat ctttaattac tgatgatagt ctcaataata
     3601 tttgtttagt tgaaagtaat ataaataagg caaaaggtga tgtctatcct ttaccacgca
     3661 caattgtaaa tgttgaaaag atgaaaccat tttgggaacg tttaatgaaa cgcggcttta
     3721 tgagtgaatc taaatatcag cgtttaataa ggatgtctga attaactgaa gaagaacggt
     3781 ttagttttat taatcgtcaa ctcgtggaaa ctagtcaagc aaataaagca gtagcgaaga
     3841 ttttagaaga tcgctatggt aaaggtagtg ttgtatatag taaagctcaa cgagtcagtg
     3901 actttagaca taaatttaaa ctactaaaac agcgtgattt aaataaagca catcatgctc
     3961 atgacgctta tcttaacata gttgtaggta attataacga tgcacaaaca aggttagcct
     4021 atattaataa acaaacgggc aagaat
//
LOCUS       DAOPCD010000073         4022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_18258_length_4022_cov_3.124023, whole genome shotgun sequence.
ACCESSION   DAOPCD010000073 DAOPCD010000000
VERSION     DAOPCD010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18258_length_4022_cov_3.124023"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="PLQ55_02600"
     CDS             complement(<1..1025)
                     /locus_tag="PLQ55_02600"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPK28854.1"
                     /translation="MRRKSLRYLGLFALLFTTLSTTTLSYTEVKGAVSAAPSRINMTI
                     YNGNANSRGFNWVTNRSVTASAVIYTRKWGEGPGDWDAPIHAPGVKNEFYPGYVSWKA
                     KIIDLIPDAAYYYRIVADDIMSPIYYFKVSSGQDEYNILHVTDTQNYVAEEYEWARKT
                     MRIATERFGLADNIIHTGDFVQTNKNPESNIIEWGYTLDIFKEFYTGTVLTPVAGNHD
                     AAHNMFTSHFNVEIPPLAETNMGFYYSYDIDDIHVTVLNTNEGLSMSTPLAANQLAWL
                     ENDLASTTATWKIVATHKGPFTTGRHALEGDIASLRNDLLPLFSEYHVDLVLQGHDHV
                     YARTNPY"
     gene            complement(1031..>4022)
                     /locus_tag="PLQ55_02605"
     CDS             complement(1031..>4022)
                     /locus_tag="PLQ55_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28855.1"
                     /translation="SNTITLELINPATYHERLVITILTPATAFVYDDISFNHELKEIN
                     DGDISYIDDTVVWSVVAGETFVEKNADNITYKAIAAGMVTFKAALKIDETVFATKEIA
                     VKARPDLDLTLAEYDSQNADANRITFDAPYAGKLYYLLADAVPTASEIISSGTQVIVS
                     EGENTFILNLGGTLGDEVDVYFVLVCMENDINIGNSEVFGLVNVPVKVIPVYVSTKDE
                     LYNALAANERAVMLSNDIDITGWVQISTAFTGILDGQGFRIHGATLVGKETGLFKTLG
                     NKAVIRNLVIDNVQAVGFTGARNAILASVIEKDATVTFDNIALTNIYMDASIEGSGRS
                     TAAALVAQYSSTSDTLTKISIKNTYINWRFDIKRNTQVSNVGSIFGTFVSAAPDALTL
                     DNVFVDAYYTGNSNNQAGLIGQVGGEIAMNNVVVHAEKFDGTNPGSLHGVDFGYLHNT
                     VTPVINETNSLLVSYTWNKLAKSDVFTLYNSVDTIPALVKQAIIDADDSKFALAEGVL
                     VMNLLSKQFRLEHAAVTPILSTYLVVNDKVVSWTAVDFAAGYEVYVNETLVSTQTTTS
                     YDLSSMSPGDYSVYVIVLGNGYDLSNTQSVNTVSVTIEEVDLDPLSAPVIVLTGTEVS
                     WAEVPNAITYQVYVNNELVQTSPNRTFSLTTYTYGEYAIKVIAIPNEGDYLPSEPSNI
                     VIYANKFEVATVSDLQTAIANNVGFISITTDLDLSTYVTPSVIFCGVLDGNNHDMTNL
                     AITTGATGFFKNLGDGAAVRNINFVSPVVTANGATMAVLSAAIDTGAHVEISNVAIIN
                     GLTNSAVVNSSSTQGCLIAAFNNAADADMQVTLNNVYVDYTHNITRAKVQNNNGAVFG
                     TLNAASEKAVIINNSYIKFMIKGWWNNAATVIGNGTANVELNNTVLTMDCADKNAGSS
                     NIYFDSGNKSMTTTLTNSLFVSDSEASFKGTAEGAVIRTTATIVLADLEMLVTGSIGG
                     WLIDTINNKATIIIAGKQYSISVG"
BASE COUNT         1246 a          853 c          736 g         1187 t
ORIGIN      
        1 tgatagggat tcgtgcgtgc ataaacatga tcgtgccctt ggagcactaa atcaacatga
       61 tactcgctaa aaagaggtaa taagtcattc cgtaatgagg caatatcacc ttctaaagca
      121 tggcgccctg tcgtaaaggg acctttatgc gtcgctacaa ttttccaagt agcggttgtg
      181 ctagctaaat cattttctaa ccacgctagt tgattagcgg caaggggtgt actcattgat
      241 aaaccttcat tcgtatttaa aacagtgaca tgaatatcat caatgtcgta agaataataa
      301 aaccccatgt ttgtttccgc aagcggcggt atttccacat taaaatgact cgtaaacata
      361 ttatgagcgg catcatgatt gcccgcgact ggagttaata ctgtccctgt ataaaattcc
      421 ttaaatatat caagagtgta accccattca ataatattag attctggatt tttattagtt
      481 tgtacgaagt cgccagtatg aataatgtta tcggctaatc caaaacgttc ggtggcaatt
      541 cgcattgttt tacgcgccca ttcatattct tccgcgacat aattttgggt gtcggttacg
      601 tgcaaaatat tatattcatc ttgtccgctg ctaactttaa aataataaat tggcgacatg
      661 atgtcatcag ccacaatgcg gtagtaatag gcggcatcag gaattaagtc aatgatttta
      721 gccttccaac tgacgtaacc tgggtaaaat tcatttttaa caccaggagc atggatcgga
      781 gcgtcccaat caccaggtcc ttccccccac ttacgtgtat aaataactgc actagccgta
      841 actgaacgat tggtaaccca attaaaacca cgagaattag cgttcccatt ataaattgtc
      901 atattgatac gcgaaggcgc tgccgaaaca gcgcctttta cttccgtata agatagagtt
      961 gtcgtactta gcgtcgtaaa gagtaaggca aataatccaa gataacgcaa tgatttacgt
     1021 cgcatattct ttatcctacg ctaattgaat attgtttacc ggcaatgata atagtcgcct
     1081 tgttattaat tgtatcaatg agccagcccc cgattgaacc agtcactaac atttctaagt
     1141 cagcaaggac aatagttgcc gtcgttcgta ttacggcccc ttccgccgtt cctttgaaac
     1201 tggcttcgct atcactaaca aagagtgaat ttgtaagggt cgtagtcatc gacttattac
     1261 cgctatcaaa atagatattc gatgatcccg cattcttatc cgcacaatcc atcgttaaaa
     1321 ccgtattgtt taattcgaca ttggcggtac cattaccaat tactgtggcc gcattgttcc
     1381 accaaccttt aatcataaat ttgatgtaac tattattaat tattacggct ttttcactag
     1441 cagcatttaa ggtgccaaag acagccccat tattgttttg aactttagcg cgggtaatat
     1501 tgtgcgtgta atcaacatat acattgttta acgttacttg catatcagcg tcggccgcat
     1561 tattaaaggc ggcgattaaa cagccttgag ttgaagaaga gttaactact gctgaattag
     1621 ttagaccgtt aataatcgca acgttactaa tttcaacatg agcacccgta tcaattgcgg
     1681 ccgaaagaac agccatcgtc gctccgttag cggtaacaac tggactaacg aagtttatat
     1741 tacgaacagc ggccccatcg cctaagtttt taaagaagcc agtcgcacca gttgtaattg
     1801 ctaagtttgt catatcatga ttattaccat caaggacgcc gcagaaaata acactcggtg
     1861 tgacataagt acttaaatct aaatcagtcg taatgctgat aaagccaaca ttattggcaa
     1921 tagcggtttg taaatcactg acggttgcaa cttcaaattt atttgcgtat ataacgatat
     1981 tggaaggttc acttggtaaa tagtcacctt cattaggaat agcaataact ttaatcgcat
     2041 attcaccata agtgtaagta gttaagctga aggtacgatt tggactagtt tggactagtt
     2101 cattattcac atatacttga taagtaattg cattgggaac ctcggcccac gatacttcag
     2161 tccccgttaa aacaataacg ggagcagata gtgggtcaag atcaacctcc tcaatagtta
     2221 cactgacggt attaaccgat tgcgtatttg ataaatcgta accattacca aggacgataa
     2281 catacacact gtagtcaccc ggactcatac tacttaaatc ataagaagtt gtcgtttggg
     2341 tactaactag cgtttcattg acgtatactt catatccagc agcaaagtcc accgccgtcc
     2401 aacttacgac tttgtcatta acgactagat aagtagagag gattggggtc acggctgcat
     2461 gttctaatct aaattgtttt gataataagt tcatcactaa gacgccttcc gcgagcgcga
     2521 acttactatc atcagcatca atgattgctt gtttaactaa ggcaggaata gtgtctacac
     2581 tattatatag tgtgaataca tcactttttg ctaatttatt ccaagtgtat gaaacgagca
     2641 aggaattcgt ttcattaata acgggcgtta cggtattgtg taaataacca aaatcaacac
     2701 catgtaatga gcctgggtta gtgccatcaa acttttcggc atgaacgacg acgttattca
     2761 tcgcaatttc accaccaact tggccgataa gacccgcttg attattggag ttaccagtat
     2821 aataagcatc aacaaagaca ttatctaaag ttagagcatc tggcgctgca ctaacgaatg
     2881 taccaaagat actgccaaca ttactaactt gggtattgcg tttgatatca aaacgccagt
     2941 taatataagt atttttaata ctgatttttg tcaaggtatc actcgtgctt gaatattgcg
     3001 ccactaaagc agcagcagtt gaacgaccgc taccctcgat gcttgcatcc atataaatat
     3061 tcgttaacgc aatattatca aaagttaccg ttgcgtcttt ttcaataacc gaggcaagga
     3121 tagcgttacg cgctccagtg aagcctaccg cttgaacatt atcaatgaca aggttacgaa
     3181 taacagcctt attacctaaa gttttaaata aaccagtttc tttacccact aacgtagcgc
     3241 catggattct aaagccttga ccatctaaaa tacctgtgaa tgccgttgaa atttgcaccc
     3301 atccagtaat atcgatgtcg ttactaagca taaccgcgcg ctcattagcg gcaagggcat
     3361 tataaagttc atctttagtc gaaacataaa caggaataac cttaactggt acattcacta
     3421 atccaaaaac ttcagaatta ccaatattaa tgtcattttc catacaaaca agcacgaaat
     3481 aaacatcgac ttcatcacct aaagtgccgc ctaaatttag gatgaaagta ttttcacctt
     3541 cacttacaat tacttgcgtt ccgctactaa ttatttcgct cgcggttgga actgcatcgg
     3601 ctaataagta ataaagtttg ccagcgtatg gcgcgtcaaa agtaatgcga ttagcatctg
     3661 cattttgcga atcatattcc gctaatgtta agtctaaatc aggacgagct tttacagcaa
     3721 tttctttcgt ggcaaaaacc gtttcatcaa ttttcagcgc ggctttgaaa gtaaccatcc
     3781 ccgcagcaat agctttatat gttatattgt cagcgttttt ctccacaaaa gtttcaccag
     3841 cgacgaccga ccaaacgaca gtgtcatcaa tataagaaat atcaccatca ttaatttctt
     3901 ttagttcatg attaaaggaa atgtcatcat aaacaaaagc agtcgctggg gttaaaatag
     3961 taatcactaa ccgttcatgg tatgttgctg gattaattaa ctccagcgta atcgtattgg
     4021 aa
//
LOCUS       DAOPCD010000074         4020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_18268_length_4020_cov_3.227995, whole genome shotgun sequence.
ACCESSION   DAOPCD010000074 DAOPCD010000000
VERSION     DAOPCD010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18268_length_4020_cov_3.227995"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..526
                     /gene="rpsT"
                     /locus_tag="PLQ55_02610"
     CDS             260..526
                     /gene="rpsT"
                     /locus_tag="PLQ55_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPK28856.1"
                     /translation="MPNIKQQIKRVKTNEKARQRNAHFKSMVRTQLKKVRVAIDAKDV
                     ALAEAEYKKAVSLLDKAAGRGVFHRNKVARHKSELARQINELSA"
     gene            complement(523..1473)
                     /gene="holA"
                     /locus_tag="PLQ55_02615"
     CDS             complement(523..1473)
                     /gene="holA"
                     /locus_tag="PLQ55_02615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPK28857.1"
                     /translation="MLYLFAANEYALVEKVIRKTVDALLPERNAFNYVRYDMRETPFS
                     EIIEDALSYAFDSSVRVIVIDHASVLTTSNEKSINKDIPDEQIDLLSRLSEDTHLIFI
                     ARSSQINPKHKIIKFIENNGKITVEKDIDKNDWQRFVRAYFAKENLPIDDEAASELLS
                     RVTNLGSFLQEAEKLAIYGKRVTLPLVEELVTPLLEENSFALVNAFISGDKAKTIKIY
                     QDFKVQNQEPVAFIAQVGNQFRTYAQIYICHEEGMTNEEIASFLKIHPYHVKLAMDQR
                     RRTSLEEVFNILLALADLDYKIKSGQIDRFYGFEMFLLNY"
     gene            complement(1476..3314)
                     /locus_tag="PLQ55_02620"
     CDS             complement(1476..3314)
                     /locus_tag="PLQ55_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPK28858.1"
                     /translation="MIISNFISKLPFESSFGLITKAGTNHIIVLTVRGKFYVRLENNK
                     LVEGDIILLNGYKVAYFFKRLEGEFNYAKYLENKGIKSALNLSFYELKVPSLLRLFNK
                     FTVKKNTYPPHLEHIKNLLLIKTINYDDPFIHELIRLDLLFLISLTGAHLSFLRRVVK
                     KISSVFVNEFLQEVISYLCLIPLFLLNITKFAFYRIFLTGILRLINKKHLNAYFNSVE
                     LTCIAAFFFLVINPYFVFDPAFYLAYLLTFLFQMVRPSRSVKGRLQSSLIVMCVIVPY
                     NIFTSGTFNLVYIFISLLLVPLIAVWTLVYFVTLFIAPLQNVGINTLNLIYHLVVLMK
                     KINITLYFGDLNVLSKLLLVTFISVLLYAFNAKIKPLQKDITLLITTLLLLNLLPLNH
                     YFEYSISFINVGQGDSTLLRIKNKYVLIDTGGLTYKDVGNDILIPYLKRNKIYRLDYL
                     IITHDDFDHCGAKEALMHNFTVKNVITERQYFPLNVNGITLENLNNKYYDDVNLDSLI
                     IYVPFPKLKILLMADAGIPNEVDLIKTYPTLEVDVLKVGHHGSNTSTSDDFIRHYKPQ
                     VAIISCGYQNKYGHPHHTVLSTLQKYDVKIKRTDTLGTITLKTCII"
     gene            complement(3470..>4020)
                     /locus_tag="PLQ55_02625"
     CDS             complement(3470..>4020)
                     /locus_tag="PLQ55_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HPK28859.1"
                     /translation="GFLFMLKLMIGVVIATVIVIFAFSVINSNMSANPQGQSSMHVID
                     DNFLSITVTGQVVKPGTYVMKKDETIGDLIMAAGGPTSNADARSYIEDTVLIAGVSYY
                     IAPINDLDDYCGEMPLHKVNINADPKDKLMEVNGIGETLAGAIIQYRGEHGEFTYLEE
                     LMKVKGIAKATFEKIKNHVTLR"
BASE COUNT         1408 a          684 c          616 g         1312 t
ORIGIN      
        1 taatatttgg gatatcttta ctacttggta tcttttatta cttgcatgac ttttttgcaa
       61 ggagaaaacg cttaaacttt aaataaagtt tttttaatca tagtatcgct ataaattact
      121 ctattgccta ctttgaagaa atttaatgaa tataatgaat agaaacgcgc aattactgaa
      181 aaattattgc actctttatt tgctaaaatt attataattt tatataatat ttaagattat
      241 tattgaaaga ggtaatagta tgccaaatat taagcaacaa attaaacgtg ttaagaccaa
      301 tgaaaaagcg cgccaacgca acgcccattt taaatcaatg gtgcgaactc aacttaaaaa
      361 agttcgcgta gcgattgatg caaaagatgt cgccttagcc gaagctgaat ataaaaaagc
      421 cgtctcttta ttagacaaag cagcaggtcg tggtgtgttc caccgcaata aagttgctcg
      481 tcataagagc gaattagcgc gtcaaattaa tgaactttct gcttagtaat taagaagaaa
      541 catttcaaat ccataaaaac ggtcaatttg gccgcttttt attttataat ctaaatccgc
      601 aagcgcgagt aaaatattaa atacttcttc taaactagtg cgtcgtcttt gatccatcgc
      661 tagtttaacg tggtaaggat gaattttcaa aaaactagca atttcttcgt ttgtcatccc
      721 ttcttcatga catatataaa tttgtgcata tgttctaaac tgattaccga cttgagcaat
      781 aaaggcgact ggttcttggt tttgcacttt aaaatcttga tatattttaa tagtcttcgc
      841 tttgtcaccg ctgataaagg cattaactag cgcaaaacta ttttcttcta aaagcggtgt
      901 tacaagttct tcaaccagtg gcaaagtaac tcttttacca taaatcgcta atttttctgc
      961 ttcctgtaaa aaagaaccta gattagtgac gcggctaagt aattcacttg cagcctcatc
     1021 atcaatcggt aaattttctt tagcaaagta agcacgcaca aagcgttgcc aatcattttt
     1081 atcaatatct ttttcaaccg ttattttccc gttattttca ataaacttaa taattttatg
     1141 tttcgggtta atttgtgaag aacgagcgat gaaaataaga tgcgtatctt cacttagacg
     1201 cgaaagcaaa tcaatttgtt cgtcaggaat atctttatta atactttttt cattacttgt
     1261 cgttaacaca ctggcgtggt cgataacaat aacacgtaca ctgctatcaa aagcataact
     1321 tagtgcatct tcaatgattt cactaaaagg cgtttctcgc atgtcgtaac gcacatagtt
     1381 aaaagcattt cgttcgggta ataaagcatc taccgttttc cgaattacct tttccactag
     1441 tgcatattca ttagccgcaa agagatataa catagttata ttatacaagt ttttaaggta
     1501 attgtaccaa gagtgtctgt tctttttatt ttcacatcat atttttgtaa agtcgaaagt
     1561 acggtgtgat gggggtgacc gtatttattt tgatatccgc agctaataat tgctacttgt
     1621 ggcttgtaat gacgaataaa gtcgtcgctt gtactcgtat ttgaaccgtg atgaccaacc
     1681 tttaaaacgt caacctctaa agtaggataa gtctttatta aatctacttc atttggtatc
     1741 ccggcgtctg ccattaaaag gatttttaac tttggaaaag gcacatagat gattaatgaa
     1801 tcaagattta catcatcata atacttatta tttaagttct ctaatgttat tccgttaaca
     1861 ttaagcggga aatattgtct ttcggtaata acatttttca ccgtaaaatt atgcattaac
     1921 gcttctttag caccacagtg atcaaagtca tcatgtgtaa taattaagta atctaaacga
     1981 taaattttat tgcgttttaa gtagggtatt aaaatatcat tgccaacatc tttataagtt
     2041 aatccccccg tatcgattaa tacatattta ttttttatcc gtagtaaagt actatcacct
     2101 tgaccgacat ttataaaact aatactatat tcaaaataat gattaagtgg taataagttt
     2161 aataaaagca atgtcgttat taatagcgta atatcttttt gtaatggttt aatcttagcg
     2221 ttaaaagcat aaagcaacac gctgataaaa gtaactaata agagtttaga aagcacattt
     2281 aaatcaccaa aatatagtgt gatattaatt tttttcatca aaacaactag gtgataaata
     2341 agattaagtg tatttatgcc aacattttgc aaaggagcaa taaaaagtgt tacaaagtaa
     2401 actagtgtcc acacagcaat aagcggcact aataaaagac taataaagat atacactaaa
     2461 ttaaatgttc cgcttgtaaa tatgttataa ggcacaataa cacacatcac gataagagag
     2521 ctttgtaacc ttccttttac tgaacgagac ggacgcacca tttgaaaaag aaatgttaat
     2581 aagtaggcta agtagaatgc gggatcaaaa acaaaataag gattaataac taaaaagaaa
     2641 aaagcagcaa tacacgttaa ttctacacta ttaaagtaag cattaaggtg cttcttatta
     2701 ataagacgca aaattcccgt taaaaagata cgataaaaag caaacttagt aatattaagt
     2761 aaaaataacg gaataagaca taagtaactg atgacttctt gaagaaactc gttaacgaac
     2821 acactgctta ttttctttac aacacggcgt aaaaacgaga gatgcgctcc tgttagacta
     2881 attagaaata ataagtctaa ccgtattaat tcatgaataa agggatcatc gtaatttatt
     2941 gttttaatta ataataaatt tttaatgtgt tcaagatgcg gaggataagt atttttttta
     3001 actgtaaact tattaaatag tcgtaataaa gacggtactt ttagttcata aaacgaaaga
     3061 ttaagcgcac ttttaattcc cttattttct aaatatttcg cgtaattaaa ttctccttca
     3121 agccgcttaa aaaagtaagc tactttataa ccgttaagta aaattatgtc cccttctaca
     3181 agtttattat tttctaaccg cacataaaat tttccacgca ctgttaaaac tataatatga
     3241 ttagtgcccg ctttagttat gagcccaaaa ctactttcaa aaggtaactt actaataaag
     3301 ttacttataa tcattataat tagacctaaa gcaaaaaaga caataacaaa agtaaaagtc
     3361 aatttatttt taaaagtgcg gactagaagc aacaagaaag gaaggataaa aagtggaagt
     3421 ctaaatgcta aaagtccgca gcaaaatgat agaaagatga ataataaatt catcgtaaag
     3481 ttacatgatt tttaattttt tcaaatgttg ctttggcaat gccttttacc ttcattagtt
     3541 cttctaaata agtaaattcg ccgtgctccc cgcgatactg aataatcgca ccagctagcg
     3601 tctcaccaat gccgttaact tccattagtt tatctttggg gtcggcgtta atattaactt
     3661 tatggagcgg catctcccca cagtaatcat ctaagtcatt aatcggagcg atgtaataac
     3721 taacaccagc aataagcact gtgtcttcaa tataagagcg cgcatcagcg ttacttgttg
     3781 gtccgcccgc tgccataatt aagtcaccaa ttgtttcgtc ttttttcatc acatatgttc
     3841 ctggttttac gacttgtccc gtcactgtta ttgataaaaa gttatcatca ataacatgca
     3901 ttgaactttg tccttgcgga ttagcgctca tattgctatt aatcacacta aaggcaaaga
     3961 tgacaataac tgtagcgatc actacaccaa tcattagctt taacataaat aaaaaacctc
//
LOCUS       DAOPCD010000075         4009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_18325_length_4009_cov_5.294638, whole genome shotgun sequence.
ACCESSION   DAOPCD010000075 DAOPCD010000000
VERSION     DAOPCD010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18325_length_4009_cov_5.294638"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..386
                     /locus_tag="PLQ55_02630"
     CDS             198..386
                     /locus_tag="PLQ55_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28860.1"
                     /translation="MDKMTFIDRTLCKLLFKMLAKKEKDKLEVWEKALLAAGNRVVDW
                     TNERYLAPITKYLANLNQ"
     gene            586..1767
                     /gene="metK"
                     /locus_tag="PLQ55_02635"
     CDS             586..1767
                     /gene="metK"
                     /locus_tag="PLQ55_02635"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010288382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPK28861.1"
                     /translation="MANYRLFTSESVSEGHPDKVCDQISDAILDAVLAINPNARVACE
                     VFATTNFVLISGETSLTKEEEPDYENVAREVIRDIGYTNPKLGFSADAAEIVIKMNQQ
                     SSDIAQGVTQIKPEEQGAGDQGIMFGYATKESAGLMPLAIVIAHKLMRVATKMRKDGE
                     FPGARPDMKSQVTMRYYEDGSSAIDTLLMSVQHDEDVNLEEFKKFIHQKIMIPVVESF
                     GKNTDFKYLVNPTGRFVIGGPAGDTGLTGRKIIVDTYGGYAKHGGGAFSGKDPSKVDR
                     SAAYYARYIAKNLVESDVCDEVEIQLSYAIGVAEPLSVAIDTFGTGKVADEVILAAIS
                     KYFDCRPGMITQEFSLRKPKFKYRDLAALGHFGRPDINIPWEETNKAQALKSYFKKYL
                     V"
     gene            1852..2412
                     /gene="raiA"
                     /locus_tag="PLQ55_02640"
     CDS             1852..2412
                     /gene="raiA"
                     /locus_tag="PLQ55_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPK28862.1"
                     /translation="MKYQIVGKNIVVTDAIRNALEKKLSRMNKYFVIDNDIDCRAVVS
                     KIRSTAKVEVTIFTKHMIFRAEESDADLYAAFDKAIDSLEGQMRKLKTRLSRRNRVGL
                     GEAIAQAIAFDNIEDATVNEAENKVVRLKSIYLEPMSLEDAIMRMEALSHKFFLYLDA
                     EDNLISLLYIRNDGGYGIIQAENKVK"
     gene            2511..3347
                     /locus_tag="PLQ55_02645"
     CDS             2511..3347
                     /locus_tag="PLQ55_02645"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPK28863.1"
                     /translation="MKKNYKLLATDLDGTLFYPKRPRSLISRKNKKFLKKFMDEGNKV
                     VLVTGRSPAYTKNLFEVLGQEVDVIGMNGAYTIVDGQIRDEHFLDFPIEKMLYDLNAL
                     FPILGTMLISQKYPLLISTPPIGKFIQFFYNVYYFLQGRYAEDYHFSNEEFIAEIESK
                     HVYKLMIFFGMSRSGVKRAARANQYLREHYPHQFEASWTGGFIEITPVGVSKANGIKK
                     YAAEKNIPEENINVVGDSGNDISMFKAFYEHSFCMKHSHKNVKKYAKTIIRRFHHLEK
                     YI"
     gene            3361..>4009
                     /locus_tag="PLQ55_02650"
     CDS             3361..>4009
                     /locus_tag="PLQ55_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28864.1"
                     /translation="MNQVTQYILGIYQINMIIRDTVSYVAPRKEQSFSKEIYEHRGRS
                     LELLTAEGSPFAHFVSINKEKADKLVENINEFKKEMYSPESRVFRVVGEGIEVDDKMH
                     HRVYEMAIGIYQTLLDVLGGYIKYAKDNDQLELRIEELVAADEYFFRSLSYFALINDI
                     FKLFKEFSDAMQQHKGEPNPIAKFINEDINKMVQLIAFMNKHNRVTNLTYKKMTDL"
BASE COUNT         1366 a          638 c          793 g         1212 t
ORIGIN      
        1 cataaaatca ttaaaacact taatcttgtt aattttaatt attcatctat cctaaattat
       61 taagcgataa aaaagtatta attttttgtg ttggtgcttc tccgtttgat gaaaaagctt
      121 ttcaaacact taaagaccat aatttaaaag atgaccttaa acatataccg tgtttctatt
      181 tgcgcggagc gtggaacatg gataagatga cattcattga tcgcacctta tgtaaattat
      241 tatttaaaat gctcgctaaa aaagaaaaag acaaacttga agtatgggaa aaagctttgc
      301 ttgctgcggg taatagggta gtggactgga ctaatgaacg ttatttagcg ccaatcacta
      361 agtatcttgc aaatctaaat caataagcat tcgtattatt ttatttcaac tatatatttt
      421 gttatactta ctttaacctt atcaagaact acattgtggc attgatgtag tagcaactct
      481 tatgaacata agtaagtgct cgctaaaggc aaatcttgta acgataagga aagtgttaaa
      541 ttactttcct tgcgaaagta attttaatta acggaggata aaattatggc taattatcgt
      601 ttattcacta gtgaatcagt aagtgaggga catcccgata aagtttgtga ccaaataagt
      661 gatgcaattc ttgatgcagt gttagcgatt aatcctaacg caagagtagc gtgtgaagtt
      721 tttgctacca ctaattttgt cttaatcagt ggtgaaacct cactaacaaa agaagaagaa
      781 ccagattatg aaaatgttgc tcgtgaagta attagagata ttggctacac aaatcctaag
      841 ttaggcttta gcgctgatgc ggcggagatt gtgattaaaa tgaatcaaca aagtagtgat
      901 attgcccaag gggtaacaca aattaaaccg gaagaacaag gcgctggtga ccaagggatt
      961 atgtttggtt acgctacaaa agaaagtgca gggttaatgc ctttagcaat tgttattgct
     1021 cataaactta tgcgtgtcgc gactaagatg cgaaaagacg gtgagttccc cggtgcaaga
     1081 cctgatatga aatcgcaagt aacgatgcgc tattatgaag atgggagctc tgcaatagat
     1141 acattattaa tgagtgtgca acatgatgaa gacgttaatc ttgaagagtt taaaaagttt
     1201 attcatcaaa agattatgat cccagtagtg gaaagttttg gcaaaaatac tgactttaaa
     1261 tatttagtta acccaacggg acgctttgtc attggtggtc ctgctggtga tactggttta
     1321 actggtcgca agattattgt cgacacttac gggggatacg cgaagcatgg tggcggtgct
     1381 tttagcggta aagacccgag taaagttgac cgcagcgctg cctattacgc gcgttatatt
     1441 gctaaaaact tagtggaaag cgatgtgtgt gacgaagtcg aaattcagct aagttacgca
     1501 atcggagtag cggagccact aagtgttgct attgatacat ttggtactgg taaagtcgcg
     1561 gatgaagtca ttttagcggc aatttccaag tattttgact gtcgtccagg aatgataact
     1621 caggaattta gcttacgaaa gccgaagttt aagtatcgcg accttgcggc gcttggtcac
     1681 tttggtcgtc ccgacattaa tattccgtgg gaagaaacta ataaagcaca agcattaaaa
     1741 tcatacttta aaaaatattt agtctaaatt aaaacttcct ctttataaat tactattctc
     1801 atgttaaaat aagaatacat aagccatctc ttgatagaaa ggaaacgata aatgaaatat
     1861 caaattgttg gcaaaaacat cgtcgtcacc gacgcaataa gaaatgcgtt agaaaagaaa
     1921 ctttcacgca tgaacaaata ctttgtcatc gacaatgaca ttgactgtcg cgcagtagtg
     1981 tcgaaaattc gctcgacagc gaaggttgaa gtaacaatct tcactaagca tatgatcttc
     2041 cgcgcagaag aaagcgatgc agatctctat gccgcctttg ataaggcaat tgattcatta
     2101 gaagggcaaa tgcggaaact taaaacgagg cttagtcgac gcaatcgcgt tggtttaggg
     2161 gaagcaattg ctcaagctat tgcctttgat aatatcgaag acgcaactgt taatgaagct
     2221 gaaaacaaag tcgtacggct taaatcaatc tatttagaac caatgtcact tgaagatgcg
     2281 attatgcgca tggaagcctt gtcgcataaa ttcttccttt atcttgatgc tgaagataat
     2341 ttaatctcat tactttatat ccgtaatgat gggggatatg gtattatcca agcagaaaat
     2401 aaagtaaaat agttaatagc gacgaagttt atcaaaaaaa tcggctagga tatcctagcc
     2461 ttttctttat ttcaatagaa ataaaaaata tattgtataa ttattacgct atgaagaaaa
     2521 attacaaatt attagcaact gatttagatg gaacactctt ctacccaaag cgaccacgtt
     2581 cgcttatttc acgaaagaat aaaaagttct taaagaagtt tatggatgaa ggcaataaag
     2641 tcgtattagt caccggaaga agtccggctt atacgaaaaa tctcttcgaa gtgcttggcc
     2701 aagaagtcga tgtgattggg atgaatgggg cctacactat tgttgatggc caaattcgtg
     2761 atgaacattt tttagatttt ccaattgaaa aaatgctcta tgatcttaat gcgctctttc
     2821 ccattcttgg tacaatgcta atctcacaaa aatatccgct tttaatctcg actccaccga
     2881 tcggcaagtt tattcaattc ttttacaatg tttattactt tttacaaggt cgctatgccg
     2941 aagattatca tttttctaat gaagaattta ttgctgaaat tgaatcaaaa catgtctaca
     3001 aattaatgat cttttttgga atgtcgcgga gtggtgttaa aagagctgcg cgcgctaatc
     3061 aatacttgcg tgaacattat ccgcatcaat ttgaagcaag ttggacaggc ggatttatcg
     3121 agattacgcc ggtaggagtt tcaaaagcta acgggattaa aaaatatgca gcggagaaaa
     3181 atattcctga agaaaatatt aatgttgttg gtgattcggg gaatgatatt tcaatgttta
     3241 aagcatttta tgagcattct ttctgtatga aacactcaca taagaatgtt aaaaaatatg
     3301 ccaaaacaat tattcgacgg ttccatcatt tagaaaaata tatttagaaa gaaggtattt
     3361 atgaatcaag ttacacaata tattttgggt atttaccaaa ttaatatgat tattcgtgat
     3421 acggtaagtt atgttgcgcc acgcaaggaa caatctttta gtaaagaaat ttatgaacac
     3481 cgcggacgta gtctagaact tttaacagca gaaggttccc cttttgcgca ctttgtatcc
     3541 attaataaag aaaaagctga caaactcgtg gaaaacatta atgaatttaa aaaagaaatg
     3601 tattcaccag agtcacgagt ctttagagta gtaggagagg gtattgaggt tgatgataaa
     3661 atgcatcacc gggtttacga aatggcgatt gggatttatc aaacactgct tgatgtttta
     3721 ggcggataca ttaaatacgc taaagataat gatcaattag aattacgaat tgaagaacta
     3781 gtggccgctg atgaatattt ctttagaagt ttatcgtact ttgcccttat taacgatatc
     3841 tttaaattat ttaaagagtt tagtgatgca atgcaacaac ataagggtga accaaatcct
     3901 attgcaaagt ttattaacga agacattaat aaaatggtgc aacttattgc ctttatgaat
     3961 aaacataatc gtgttactaa cttaacttat aagaaaatga ccgatttaa
//
LOCUS       DAOPCD010000076         4009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_18326_length_4009_cov_4.132777, whole genome shotgun sequence.
ACCESSION   DAOPCD010000076 DAOPCD010000000
VERSION     DAOPCD010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18326_length_4009_cov_4.132777"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2037
                     /locus_tag="PLQ55_02655"
     CDS             <1..2037
                     /locus_tag="PLQ55_02655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF001688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HPK28865.1"
                     /translation="STTEMFEEMRKFGVFTEEELADMIINNPHRINDLVENITPFTKE
                     LYKPSIENVDELLENLVFETAYEIYGNPLPTLISDRIHRELTGIINNGYAVIYYLAHL
                     IVKKANDEGFIVGSRGSVGSSVVAYFAKVSEVNPLPPHYFCPHCKFSNFRNEQRVLSG
                     YDLPLAKCPECGHDLVGEGQNIPFETFLGFEADKIPDIDLNFPEDFQKEAHNFTKELV
                     GENNAFRAGTIQTVAERTAFGHVLNYYEKQGIYRDELNNENVAYLAYHIAGVKRTTGQ
                     HPGGIVVIPSEYEVYDFTPVQYPAGNTESDWLTTHFDYDSIHDNLLKLDLLGHKDPVT
                     LKMLCDLTGIKFEDVPTNDRATISLFSSHEALNLKSNPLGEVTGALGLPEFGTNFVRQ
                     LLTEAKPKTFNDLVIVSGLSHGTGVWRFNADELIRSEQATLGDVIGCRDDIMTFLIRQ
                     NLHEKDAFNIMESVRRGRGVEEGLETKMREAGVPHYYIESCKKIEYLFPRAHAAAYVI
                     MAVKVAWFKLNYPLEYYATFFTIRGDNFDLKTMISSEEVILKELQKFEEQRKTSELNP
                     RDSNIVENLQLTIEMLNRGFKISNIDLYKSEATRFKVDHENNQIIPPFIVIRALGEGT
                     AESVVEARKNGEFISIEDLVERTRLNTSNIENLKELGALEGLPESNQISLFDFM"
     gene            complement(2039..2947)
                     /locus_tag="PLQ55_02660"
     CDS             complement(2039..2947)
                     /locus_tag="PLQ55_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28866.1"
                     /translation="MNKESGYRKTIISEYLNNLLHALNAYEGYSDGFRKLNGDFINYL
                     QARWVDLNIEVGDAIRVKFQRDRDYLSHPFLGIVIRLLEGARIHVPQREETLALLKKH
                     TNYPLHNFITFAFNFNRNDKDAPFEILSDDFIKIIFYLTNKKGYPHLENFDSFLEYKL
                     RISGVSIINLVYARRSALVEKNIDKVTEHLSLAAIQSGQDHRKFLGQKYYELATLFKK
                     HNDFAGKLLQKAVDFKNYEALLPLVKYYEEASDDKDDYTAIAFYYLVIAHHLEIPGAG
                     EKLKTYYEKGGVRPPCNKMLSKFFED"
     gene            3061..3137
                     /locus_tag="PLQ55_02665"
     tRNA            3061..3137
                     /locus_tag="PLQ55_02665"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3095..3097,aa:Pro,seq:cgg)
     gene            3266..3352
                     /locus_tag="PLQ55_02670"
     tRNA            3266..3352
                     /locus_tag="PLQ55_02670"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3300..3302,aa:Leu,seq:caa)
     gene            3548..3937
                     /locus_tag="PLQ55_02675"
     CDS             3548..3937
                     /locus_tag="PLQ55_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28867.1"
                     /translation="MNKTNLKNKISKRIIISEIIYVVVTAIISLLMFLPIYKDRATLP
                     GYDEEGNQIVVNLFYEKTPYQRLKAINIEWLLYLGLSLFLICIVILIISYTTNHKLDK
                     YKKVIFIISFGLILVLLLIAAIQITMY"
BASE COUNT         1323 a          638 c          733 g         1315 t
ORIGIN      
        1 tctacaactg agatgtttga agaaatgcgt aagtttggtg tatttaccga agaagaatta
       61 gcagatatga ttattaataa tccgcaccgg attaatgatt tagtggaaaa tattacgcca
      121 tttacaaaag aattatataa accatcaatt gaaaatgttg atgaactttt agaaaactta
      181 gtgtttgaaa cagcttatga aatttacggt aatccattac caactttaat tagtgaccgt
      241 attcatcgtg aattaacagg gattataaat aatggctatg cagttattta ctacttagcg
      301 catctaattg ttaaaaaggc aaatgacgaa ggatttattg ttggtagccg cggtagtgtt
      361 ggctcaagcg tcgttgctta tttcgctaaa gtctcagaag ttaatccgct cccaccgcat
      421 tacttctgcc ctcattgtaa atttagtaat ttccgtaatg aacaaagagt actctcgggt
      481 tatgacttac cactcgctaa atgtcctgaa tgcggacatg atttagttgg tgaagggcaa
      541 aatattcctt ttgaaacatt ccttggtttt gaagctgata aaattccgga tattgacctt
      601 aacttccctg aagactttca aaaagaagca cataacttca ctaaagaact tgttggtgaa
      661 aataatgctt ttagagcagg aacgattcaa actgtggccg aacggacagc gtttggtcat
      721 gtcttaaatt attacgaaaa acaagggata tatcgtgatg aattaaataa tgaaaatgtt
      781 gcttatcttg cttatcacat cgctggtgtt aaacgtacaa cagggcaaca cccgggtggg
      841 attgttgtaa ttccaagtga atatgaagta tatgacttta cacccgtaca atatccagcg
      901 gggaacacgg aaagcgactg gttaacaacg cactttgact atgattcaat tcatgataac
      961 ttattaaaac tagatttatt aggtcacaaa gatccagtaa cacttaagat gctttgtgat
     1021 ttaaccggta ttaagtttga agatgtgccc actaatgacc gtgcaacaat ttcgttattc
     1081 tcaagtcatg aagcacttaa cttaaaaagt aatccgcttg gcgaagtaac aggtgcttta
     1141 ggtttacctg aatttggcac taactttgtt cgtcaattat taacggaagc aaaaccaaaa
     1201 acatttaatg atcttgtgat tgtttcaggt ttaagccacg gaactggcgt gtggcgcttt
     1261 aacgctgatg aattaattag gtcagagcaa gcaacacttg gagatgttat tgggtgccgt
     1321 gatgatatta tgacattttt aattcgccaa aatttacatg aaaaagatgc ctttaacatc
     1381 atggaatcag tgcgaagggg acgcggtgtt gaagagggtc ttgaaacgaa gatgcgtgaa
     1441 gctggtgttc cgcactatta cattgaatca tgtaagaaaa ttgaatatct cttcccacgg
     1501 gcgcacgctg cagcttatgt tattatggca gtgaaagtag cttggtttaa acttaattac
     1561 ccgcttgagt attatgcaac attctttaca atccgcggtg ataactttga tcttaaaaca
     1621 atgataagta gcgaagaagt tatcttaaaa gagttgcaaa aatttgagga acagcgtaaa
     1681 acatcggaat taaatccgcg cgacagtaat attgttgaaa acttacagtt aacaattgag
     1741 atgcttaatc gtggctttaa aatttcgaat attgatctat ataagagtga ggctactcgg
     1801 tttaaagttg atcatgaaaa taatcaaatc attccgccct ttatcgtcat tagagcactt
     1861 ggcgaaggca ctgctgaaag cgtggttgaa gcacggaaga atggtgaatt tatttcgatt
     1921 gaagatttag tagaaagaac acgtttaaac acttcaaaca ttgaaaactt aaaagaacta
     1981 ggagcgctag agggtctccc agaaagtaat caaattagct tatttgactt tatgtaaatt
     2041 agtcttcaaa aaatttactt aacatcttgt tacacggagg ccttacgcct cctttttcat
     2101 agtaagtctt taatttttct ccggcaccag gtatttcaag gtgatgagca attacaagat
     2161 aataaaatgc aatagcggta tagtcatcct tatcgtcact tgcttcttca taatatttaa
     2221 caagtggtaa tagtgcctca taatttttaa agtcaacggc cttttgcaac aattttccgg
     2281 caaaatcgtt gtgtttttta aataatgtcg ctagttcata gtacttttga cctaagaatt
     2341 tacggtggtc ttgacctgac tgtattgctg ctagggataa gtgttctgtt actttgtcga
     2401 tatttttttc gacaagcgca ctgcgtctag catatacgag gttaattata ctaaccccgc
     2461 taatacgtaa tttatattct aagaaactat caaagttttc taaatgcgga taaccttttt
     2521 tatttgttaa gtaaaagata atcttaataa aatcatcgct taatatttca aaaggagcat
     2581 ctttgtcatt gcgattaaaa ttaaaggcaa aggtaataaa attatgaagc ggataattcg
     2641 tgtgtttttt tagtaacgct agcgtttctt cgcgctgcgg gacatgaatc ctagctcctt
     2701 caagtaaacg aataacaata cctaaaaagg gatgcgataa ataatcacgg tcacgctgaa
     2761 acttaacccg aatagcatca ccgacttcga tatttagatc gacccagcga gcttgtaaat
     2821 aattaataaa atcaccgttt agtttacgga aaccatcact atatccttca taggcattaa
     2881 gtgcatgtaa aagattattt aaatactcgc ttattatagt tttccgatac cctgattctt
     2941 tattcatata aatattttat caaattttaa ggttatcatt gcgtgtatag ataaaatcaa
     3001 cagtgtttat gacacaaaag tttggtaatt tttaaaaaca gcgtataata tgtgtgcagt
     3061 cgggatgtag cacagcttgg tagtgcgtct ggttcgggac cagaaggtcg caggttcgaa
     3121 tcctgtcatt ccgaccattt ttattttaaa gaaatgatgt tcattgaaaa aaagtgtgac
     3181 taatcacata atttctcagc gaaggtttta atgaatttag ctttataaaa aaatttatgt
     3241 tgttaatgtt ttataattat cttgtgcctc cgtggtggaa gtggtagacg cgatggactc
     3301 aaaatccatt agtagagata ctgtatcggt tcaagtccga tcggaggcac caaaaaattc
     3361 tgaaacaccg gttttaacta ttaaaactga gaaataagta gctattgatt aatttcatgg
     3421 ttactttttt tattttaagt atttctttaa atgtacatat tagaattttc ttgtatcatt
     3481 tcttgtatcg tttcatgtat catttcaaaa tcaagtgtga taagattata ctaagaagtg
     3541 gaggcatatg aataaaacaa atttaaaaaa taaaataagc aaaaggataa ttattagcga
     3601 aattatttat gttgtagtta cagcaataat ttctttgctc atgttcttgc caatatataa
     3661 agaccgagct actttgccag gatatgatga agaaggtaac caaattgttg ttaatttatt
     3721 ttatgaaaaa actccttatc aaagactcaa agcaatcaat atcgagtggt tattgtattt
     3781 aggactttca ctatttctta tttgcatagt tatattaatc atttcttata ccactaatca
     3841 taaactagat aaatacaaaa aagtgatttt tataatatca tttggtctta ttcttgtttt
     3901 attgctgatt gctgccatcc aaataacaat gtattgagat tattttctaa atatatagac
     3961 cattatattc actttttaaa ggcaaaggag taaaacattg tgccatatt
//
LOCUS       DAOPCD010000077         4009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_18328_length_4009_cov_3.030855, whole genome shotgun sequence.
ACCESSION   DAOPCD010000077 DAOPCD010000000
VERSION     DAOPCD010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18328_length_4009_cov_3.030855"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..2160)
                     /locus_tag="PLQ55_02680"
     CDS             complement(94..2160)
                     /locus_tag="PLQ55_02680"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPK28868.1"
                     /translation="MSYTTDKIRNIIFLGHQGSGKTTLVEQIASNILKQPAGSVERKN
                     TISDFTPEEKARLTSCSMSVVSLDYNGYRLNLLDAPGNDDFVADVVGVLDFVKAAVLV
                     VDTGKIEVGTVKHFNLLKKYGVPTFIFVNKLDKELVNFDEVLEKITGTLGKKGVSFTY
                     PLNDGKVFNGFVDVTTLKAVKADGSKRVEVELDEATKAKVSEMNNALVEQVALTDDAL
                     LEKFFSGEELSAEEIANGLKKAVLSGEIAPVIVGSSTENIGVNTLVDMALTYLPSPAF
                     IKEMKGKDRGNNEVVRAISENGPFSAYVFKTVYDQYKGTTNYAFVVSGSLELGSDVYN
                     AELNSTMRASQMFSLLGAKQNNIQKAIAGDIVAFAKLDGITTGHTLCDKNDVVTFPRA
                     NYPTIVYRRAISAKNSRDEEKLVQALYRVAAEDPTLEITRVPETKQLLLGGLSDSHLN
                     FVLDKVKTTFGFEANTDAPQVIYRETVKREASAVGRYVKQSGGSGFYGVVEMRFGPSG
                     SDENVFTEEIFGGTVPKNYHPAVEKGFFESVQEGQLAGFPVIGMKATLFDGKYHSVDS
                     NEQAFRMAAILAYREAYMNCAPTLLEPIMKITISVTNEFTGNVMNDLNQRRARVLSMD
                     ESGYGTQEITALVPEAELLDYAIKLRVLSQGSGSFNSEFDSYQEVPTNLLQGVIAANS
                     RLEKDK"
     gene            complement(2176..2727)
                     /locus_tag="PLQ55_02685"
     CDS             complement(2176..2727)
                     /locus_tag="PLQ55_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28869.1"
                     /translation="MAKIIIISGDLAAGKSVLAKKLSRHYQIPYFSKARFKEILGEKI
                     SFEGIDENKNISRSSYALLRHIGWRVAQVKGSVILEANFHTEHLEQIKRDVEFTGHKV
                     ELLYLTGDLDVLFERFLYREHYENRHPVHLTHPLRSLEEFENYILKLRNEPEVLPRKM
                     LDTTNCDQEMIFEKALKLIGSLD"
     gene            complement(2796..3671)
                     /locus_tag="PLQ55_02690"
     CDS             complement(2796..3671)
                     /locus_tag="PLQ55_02690"
                     /inference="COORDINATES: protein motif:HMM:NF021471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HPK28870.1"
                     /translation="MKKCVFYTALSGLALTIFGLIVAFFGYRLDLFVEAIPHLVGLFP
                     ALWWVILPPAVGLLVLIINIAVKAKKKNKCAIVPFALFVSILAYLLVVPRALPEWLAE
                     GVAPESLVFTTGAAVVVIVFLVLFVLAFVDMALPTRKQLRAEKELDEEVYEVVEEKPT
                     RGPHDAPVLYSDEEVEDEEGLEEVSEAERKVAEKPKAKKAKAKETEKAKEEEEEVKKE
                     DVKKEKEYDRIYHIMKRAKDDRWIVKIAQSRKAIKIFDTQKEAIAYAEILASNNNGVV
                     RVFASKGANKGRIIQ"
BASE COUNT         1158 a          846 c          696 g         1309 t
ORIGIN      
        1 gtgtcaaaag caaatagaaa ctacaaaata aagcgattat atacaaaaaa ccatcggcgc
       61 acaccgatgg ttttctttat gtaaactatc taattattta tctttttcaa gccgtgaatt
      121 ggcagcgata acaccttgta ataagtttgt gggtacttct tgatagctat caaattcact
      181 attgaaacta ccactgcctt ggcttaagac ccgaagttta atggcgtagt ctaatagttc
      241 tgcttcaggc actaaggcgg taatttcttg ggtaccataa ccactttcat ccatacttaa
      301 tacccgtgcc cgacgttggt taaggtcatt cataacgtta cccgtaaatt catttgtgac
      361 actgatggta attttcataa tcggttcaag taaggtcggg gcacaattca tataagcttc
      421 gcgataggct aaaatagcgg ccatgcggaa cgcttgttcg ttactatcaa cgctgtggta
      481 tttaccgtca aataatgttg ctttcatccc gattacgggg aagcccgcaa gttgaccttc
      541 ttgcacactt tcaaagaatc ctttttcgac agcagggtga tagttctttg gtactgttcc
      601 cccgaagatt tcttcggtaa agacattttc atcactacca cttgggccaa agcgcatttc
      661 aacaactccg tagaagccgc tgccgcctga ttgcttaaca taacggccaa ctgcactagc
      721 ttcgcgtttt accgtttcac ggtaaattac ttgtggtgca tcggtatttg cttcaaagcc
      781 aaatgtagtt tttactttat caagcacaaa gttaagatga ctatcactaa gtccaccaag
      841 taagagttgt tttgtttctg gaacacgcgt aatttcaagg gttgggtctt ctgcggcaac
      901 acggtagagt gcttgcacta acttttcttc gtcacgacta tttttcgcac tgatagcgcg
      961 gcgataaaca atcgtcggat aattagcgcg cgggaatgta acgacatcat ttttatcaca
     1021 taatgtgtga ccagtggtaa tcccgtctaa ctttgcaaaa gcaacgatat caccagcaat
     1081 cgctttttga atgttatttt gtttagcgcc taaaagacta aacatttgtg aagcacgcat
     1141 cgtggaatta agttcagcat tatacacatc actaccaagt tctaaactac cacttacgac
     1201 aaaggcatag ttagtagtgc ctttatattg gtcatatact gttttaaaga cataagcact
     1261 aaaaggtcca ttttcactaa ttgcacgcac aacttcatta ttaccgcgat ccttgccttt
     1321 catctcttta atgaaagctg ggcttggtaa gtaagtaagt gccatatcaa caagtgtatt
     1381 aacaccgata ttttcggtac tgctaccaac aataactgga gcaatttcac cgcttaacac
     1441 ggccttttta agtccgttag cgatttcttc agcgcttaac tcttcaccgc taaagaattt
     1501 ttctaaaagg gcgtcatccg ttaacgctac ttgttcaaca agcgcattat tcatttcgct
     1561 gactttcgct ttagtcgctt catcaagttc aacttcaacg cgttttgacc catctgcctt
     1621 aacagcttta agcgttgtga cgtcgacaaa accgttaaat actttaccgt cattaagcgg
     1681 ataagtgaag cttacgcctt ttttaccgag tgtaccagta attttttcaa gaacttcatc
     1741 aaagttaact aattctttgt ctaacttatt aacaaaaata aaagtaggaa cgccatactt
     1801 ctttaataaa ttaaagtgtt taactgttcc gacttcaatc ttaccagtat cgacgactaa
     1861 gactgctgct ttaacaaagt caagcacacc aacaacatca gcaacaaagt catcattgcc
     1921 tggtgcatca agtaagttta agcgatagcc attataatca aggctaacaa ccgacattga
     1981 acatgatgtt aaacgcgctt tttcttcagg tgtaaagtca ctaatggtat ttttccgttc
     2041 aacactgcca gcgggctgtt ttaagatatt acttgcgatt tgttccacaa gtgtcgtttt
     2101 tcctgagccc tggtggccaa ggaagataat gtttctaatt ttatctgtgg tataactcat
     2161 aatttctcct tcatattaat ctaaactacc aatgagtttg agtgcttttt caaaaatcat
     2221 ttcttgatca caattagtgg tatcaagcat cttgcgtggt aatacttcag gttcattacg
     2281 taattttaaa atataatttt caaattcttc taatgaacgt agtggatgag ttaagtgcac
     2341 tggatggcga ttttcgtaat gttcacgata taaaaatctt tcaaatagta catctaaatc
     2401 acccgttaaa tagagcaact caactttgtg accagtaaat tcaacgtcac gtttaatttg
     2461 ttctaagtgt tcagtgtgaa aattagcttc taaaataaca ctgcccttta cttgggctac
     2521 ccgccaccca atatggcgta agagcgcata tgaggagcgc gaaatatttt tattttcgtc
     2581 gattccttca aacgaaattt tttcgccgag tatttcttta aaccgagctt ttgagaaata
     2641 agggatctga taatgacgtg aaagcttttt tgctaacaca cttttaccag cggcgaggtc
     2701 acctgaaata attatgattt tggccatgct ttaatattcc tttctactta gcacaatata
     2761 taaaaaggca ccttgtgtta agtgccttct ttttactact gaataatgcg ccctttattg
     2821 gcgccttttg aggcgaagac acgaacaact ccgttgttgt tactcgctaa aatttcagca
     2881 tatgcaatcg cttctttttg tgtatcaaag attttaattg ctttgcggct ttgggcaatt
     2941 ttaacaatcc aacgatcatc tttcgcccgt ttcatgatgt gataaatgcg gtcgtattct
     3001 ttttctttct ttacgtcttc ttttttcact tcttcttcct cttcctttgc tttttctgtt
     3061 tcttttgctt ttgctttttt cgcttttggt ttttctgcga ctttgcgttc agcttcgcta
     3121 acttcttcta agccttcttc gtcttcgact tcttcatcac tgtatagtac tggagcgtca
     3181 tgtggtccgc gtgttggctt ttcttcaact acttcataaa cttcttcatc aagttctttt
     3241 tcagcgcgaa gttgtttccg tgttgggagt gccatatcaa cgaacgctaa gacaaagagc
     3301 actaaaaata caatgacgac aactgctgca ccagtcgtaa acactaaact ttctggtgct
     3361 acgccttctg ctaaccattc tggtaatgcg cgtggcacta cgagtaagta agcaagaata
     3421 gacacaaata gcgcaaacgg aacgattgca catttatttt tcttcttggc tttaacagcg
     3481 atgttaataa ttaacaccaa aagtcccacg gctggcggta aaattaccca ccataatgcg
     3541 ggaaataatc caactaaatg gggaattgcc tctacaaaga gatcaagccg ataaccaaaa
     3601 aaggccacga tgagaccaaa gattgttaag gcgagaccac taagagcggt ataaaaaaca
     3661 catttcttca taagttctcc ttttcctaat ataaaactca atagtattat ataaaaataa
     3721 tactcagaag taaacaaaaa ggggctaaaa taccgaatat ttgccccatt ttgcttttat
     3781 ttaaagtagt aagcgcttta ttctttactt tccttttaag gttgtaaaac ttcactgcca
     3841 taagtatcca tatttttata ggcgtgcgct acatattcaa agtgaacaaa ataatattaa
     3901 attttagtat caattataac ttgggctttt agcacaacat tatatctcgg cattgcactt
     3961 tgtgtaaatg tcataaaagt cggttgccag taagtactaa cttcacctt
//
LOCUS       DAOPCD010000078         3938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_18738_length_3938_cov_3.143961, whole genome shotgun sequence.
ACCESSION   DAOPCD010000078 DAOPCD010000000
VERSION     DAOPCD010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18738_length_3938_cov_3.143961"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="PLQ55_02695"
     CDS             complement(<1..1141)
                     /locus_tag="PLQ55_02695"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPK28871.1"
                     /translation="MEFGKYNIDKRLVDTLKMHGYVTMTGVQSEILPRALKGESLIVK
                     AKTGSGKTHSYLIPILNKLEKTEELQALILAPTRELARQIYDFARVLNDDYKGVDILF
                     LAGGLQTSRHKERLASKPALIIATPGRLKALLINEDITSFKSIHTVVLDEGDMLLDSG
                     FYDVISETLAALDNPTVQLFSATIPQKLANLVTLKTGVKTVIDIDRDDKTSDNVTHHL
                     IDVHHMNRFEVVESFVKHFNPYLLLVFCSTNKEVLELYEFLSAKGHKIGLLTGELESR
                     KRKAMFRRVRNNEFQVVVASDIAARGIDIEHVSDVLSVSFPFDLEFYFHRAGRTGRLD
                     NKGNAYTLYDHDDLETVEKVEKLGVKFTRLVYKGGAFSAVTKRISR"
     gene            complement(1206..1979)
                     /locus_tag="PLQ55_02700"
     CDS             complement(1206..1979)
                     /locus_tag="PLQ55_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPK28872.1"
                     /translation="MKTRTLLTKLGRLYPKRIALKHNDYVGYMAGQKVEEVKRIVLLL
                     DLDEIVLEEALSHKPDLILTHHPFIRGTRAKVFARDEKKKAFALWLDTLPVTVYSMHT
                     NFDEAPNGMNDALAEKLELSAVKPLASEPMARGGYLPYPMTVEEAANYIVDKLEATYG
                     LLVAGDKEREIKRVAIIGGGGSRDWPAAMAEGYDMYISGDAPHYVRRDVMNAGYNYLD
                     VPHEIENAFLWKMKEVLLTIDPSFEVIAIKHEKPPVVIK"
     gene            complement(1976..2644)
                     /locus_tag="PLQ55_02705"
     CDS             complement(1976..2644)
                     /locus_tag="PLQ55_02705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK28873.1"
                     /translation="MLSLRLQAIANLIKKDDVVADIGSDHGLLVNYLAIKGHRALYAS
                     ENKKGPYNRLKSELKAYKSDIIEIDLADGLENLPARINTVVIAGMGGELIVSILQAHP
                     AKLVNVQKLILAPHCNAKAVREVLNALNYSISYEEIVVEKEVFYEIIVAEQKAIATKE
                     VNPYFGTYHLQARSENFIKMWTSVYNQNKRILSNKKLPLSRKEELLKEQELIEKVISE
                     GEKV"
     gene            complement(2644..>3938)
                     /locus_tag="PLQ55_02710"
     CDS             complement(2644..>3938)
                     /locus_tag="PLQ55_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPK28874.1"
                     /translation="DATSHLDLSENEFDKLIQYFKNNKVTIHSDIESDEYDPDFANFV
                     DGDDDLDDDLDLDDDFDEEELSFGDAFKDVKEENFEENQRLRKISLGEVKTTDSVKIY
                     MKDMGRYSLLKADEEVTLARRYKEEGDIDARDELITRNLRLVFNEAKKYSSGNMHILD
                     LIQEGNIGLMKAVEKFDYTKGFKFSTYATWWIKQSISRSIADQARTIRIPVHMVETIN
                     KISRTQRRLTQEFGRDATPEEISDALIKYSVKDVEEAMELSANATEENPIPEEVANKV
                     RTIMAKVETLTEELKRPATPEEVSESFREFTPARIREIQAIAMEPVSLESPIGEEDDS
                     HLGDFVEDKDNETPTDFVRKSILHDQLRDNMQEFLTPREERVLHLRYGFDDDKPRTLE
                     EVGKIFGVTRERIRQIEAKAIKKLRHPSRTKGLRGHIQ"
BASE COUNT         1115 a          839 c          657 g         1327 t
ORIGIN      
        1 tgcgactaat gcgcttggtt acagcactaa aagcgccacc tttatacaca agacgcgtaa
       61 atttaacacc taacttctct actttttcga ctgtttctaa gtcatcatga tcgtaaagcg
      121 tataagcatt acctttatta tctaaacgcc ctgttctacc cgcacggtgg aagtaaaatt
      181 ctaaatcaaa aggaaaacta acgctaagaa catcactaac atgttcaata tcaatcccgc
      241 gcgctgcaat atcactcgca acaacaactt ggaattcatt attacgcacc cgtctaaaca
      301 ttgctttacg tttacggctt tcaagctcac cagttaaaag tccgatttta tgaccttttg
      361 cacttaaaaa ctcataaagt tcaagcactt ctttattcgt ggagcaaaac actagtaata
      421 aataaggatt aaagtgttta acaaaacttt cgacaacttc aaagcgattc atatggtgta
      481 catcaattaa gtgatgggtg acgttatcac ttgttttatc atcgcggtca atatcaatca
      541 cggtctttac acctgtcttt aaagtaacaa ggttcgctag cttttgcgga atagtcgccg
      601 agaaaagctg gacagtagga ttatcaagcg ctgctaaagt ttcggaaata acatcgtaaa
      661 aacctgaatc taaaagcata tcaccttcat cgagcacgac agtatgaatt gatttaaaac
      721 ttgtgatatc ttcgttaatt aataaagctt ttaatcggcc aggcgtcgca ataattagcg
      781 ctggtttact cgctaaacgt tctttatgac gactagtttg gagtccgccg gcaagaaaca
      841 atatatctac tcctttatag tcatcattta aaacacgagc aaaatcatag atttgccgcg
      901 ctaattcgcg ggttggtgct aagatcagtg cttgaagttc ttcggtcttc tctaatttat
      961 ttaaaattgg tattaaataa ctatgagttt tcccgctacc cgtcttcgct ttaacgatga
     1021 gtgattcacc ttttaaagcg cgtggcaata tttcgctttg cacaccggtc attgttacat
     1081 aaccgtgcat ttttaaagtg tccactaatc ttttatcaat attgtattta ccaaactcca
     1141 taatatcaat tccttattat ctactacata actactaatt tattacttat aaaatacttt
     1201 taccgttatt ttataacaac tggcggtttt tcgtgcttaa ttgcaattac ttcaaaactt
     1261 ggatcaattg ttagtaagac ttctttcatt ttccataaga atgcattctc tatttcatgg
     1321 ggtacatcta aataattgta gccagcattc attacatcac ggcgcacata atgcggtgcg
     1381 tcaccactta tatacatgtc gtagccttca gccattgctg ctggccaatc gcgacttccg
     1441 ccaccaccaa tgatagcgac tcgcttaatt tcgcgctcct tatcaccagc gactagtaag
     1501 ccgtaagttg cttctagttt atcaacaata tagttagcag cttcttcaac tgtcattggg
     1561 tacggtaaat aaccgccgcg cgccattggt tcgctggcta atggtttaac ggcgcttaat
     1621 tctaattttt ccgcgagggc atcattcatc ccgtttggtg cttcatcaaa gttcgtatgc
     1681 attgaataaa cagtcacggg taaagtatca agccatagcg caaatgcttt tttcttctca
     1741 tcgcgcgcaa acactttagc gcgtgtaccc ctaatgaacg gatgatgggt taaaattaag
     1801 tcaggtttat gacttaatgc ttcttcaagg acaatttcat ctaaatcaag tagtaacaca
     1861 atgcgtttaa cttcttcgac tttttgccct gccatatagc caacataatc attatgttta
     1921 agggcgatac gctttggata aagacgacca agtttagtta gtaatgtgcg tgtcttcata
     1981 ctttttctcc ttcactaata actttttcaa taagttcttg ttcttttagc aactcttctt
     2041 tacgacttag tggtaacttt ttattactta agatgcgttt attttggtta taaacactag
     2101 tccacatctt aataaagttt tcacttctcg cttgtaagtg ataagtgcca aaatacggat
     2161 taacttcttt tgtcgctatc gctttttgct cggcaacaat tatttcgtaa aacacttctt
     2221 tctcgacaac gatttcttcg tagctaattg aatagtttaa cgcatttaat acttcgcgca
     2281 ctgctttagc gttacagtgc ggcgctaaaa tgagtttttg gacattaact aacttagcgg
     2341 gatgcgcctg taagatactt acaattaatt cgccacccat accagcgata acgacagtgt
     2401 taatgcgtgc aggtaagttt tcaaggccgt cggctaaatc gatttcaata atgtcactct
     2461 tataagcttt aagttcgctt tttaaccggt tgtatggtcc ttttttattt tcgctcgcat
     2521 ataaagcacg atgtccttta atcgctaaat aattaacgag taaaccgtgg tcactcccaa
     2581 tatcagcgac aacatcatct tttttaatta aattggcaat tgcttgtaaa cgcagcgata
     2641 acattattgg atatgtccgc gtaacccttt cgtacgcgaa ggatgacgta attttttaat
     2701 cgctttagct tcaatttgac gaatccgttc tcgtgtaaca ccaaaaatct tcccaacttc
     2761 ttcaagtgtc cgcggtttat catcatcaaa gccgtagcgt aagtgtaaca cacgttcttc
     2821 gcgtggtgtg agaaattctt gcatgttatc acgtaattga tcgtgtaaaa tgctctttct
     2881 aacaaagtcg gttggtgttt cattatcttt atcttcgaca aaatcaccta aatgactatc
     2941 atcttcttca ccgattggtg attctaaact tactggttcc atcgcaattg cttgaatttc
     3001 acgaattctc gcaggtgtaa attcacggaa tgattcactt acttcttctg gtgttgctgg
     3061 ccgtttaagt tcttcagtta aagtttcaac tttcgccata atagtacgca ctttattggc
     3121 gacttcttcc ggaatcggat tttcttcggt tgcgttagcg cttaattcca ttgcttcttc
     3181 aacatcctta acgctatatt taattaaagc atcactaatt tcttcaggtg tagcgtcgcg
     3241 cccaaattct tgggtaaggc ggcgctgtgt ccggctaatt ttattaattg tttcaaccat
     3301 atgaacagga atacgaatag tgcgggcttg gtccgcgatt gaacgactaa tcgactgctt
     3361 aatccaccag gttgcataag ttgagaactt aaaacccttt gtgtaatcaa acttttcgac
     3421 agctttcatt agcccaatat taccttcttg aattaaatca agaatatgca tgttgccgct
     3481 gctgtatttt ttggcttcat taaaaacaag tcgtaagttg cgagtaatta attcatcacg
     3541 cgcatcgata tcgccttctt ctttataacg acgggcaagt gttacttctt cgtcagcttt
     3601 aagtaaacta tagcgcccca tgtctttcat atagatttta acggagtctg ttgttttaac
     3661 ttcaccgagt gaaattttac gtaaccgttg attttcttca aagttttctt ctttaacatc
     3721 tttaaaggca tcaccaaatg ataattcttc ttcatcaaaa tcatcatcaa gatcaagatc
     3781 atcatctaaa tcatcgtcac catcaacaaa attagcgaaa tctggatcat attcatcgct
     3841 ttcaatatca ctatgaatag tcactttgtt atttttaaag tattgaataa gtttatcaaa
     3901 ctcattttcg cttaagtcta ggtgtgaagt cgcatcca
//
LOCUS       DAOPCD010000079         3924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_18820_length_3924_cov_3.249935, whole genome shotgun sequence.
ACCESSION   DAOPCD010000079 DAOPCD010000000
VERSION     DAOPCD010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18820_length_3924_cov_3.249935"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2503)
                     /gene="ileS"
                     /locus_tag="PLQ55_02715"
     CDS             complement(<1..2503)
                     /gene="ileS"
                     /locus_tag="PLQ55_02715"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPK28875.1"
                     /translation="MELKDTLLMPKTKFEMRGNLTRKEPGILARWEAIDLYKLMVKKN
                     ETSKPFILHDGPPYANGNIHSGHMLNRNLKDFVVRMKNMQGYDTPFIFGWDTHGLPIE
                     NQVTKSGVNRKAIPITEFRKLCKEYALKQVEIQKAQLKRLGLVGDFENAYLTLTKDYE
                     ADELRVFKRMALKGLIYRGLKPVQWSPSSETALAEAEIEYKDVVSDSIYVLFNVTKGN
                     DKVPVGTKFMIWTTTPWTLPANLAISVHPAFTYGVYESALGDVILLPELVEEVSKASD
                     LTFTLKYEVKGADLDLIETKHPLYERISLVITGMHVTSESGTGAVHTAPGHGLEDFVV
                     GQKYGLDVLCPVDARGYFTSEAGKYEGLFYEDANKVIINDLREVGALVAHTKVKHSYP
                     HDWRTDKPLIFRATPQWFASIEPIRRDLLNAVQKVDWYPAWGEGRISNMISDRGDWTI
                     SRQRVWGVPIPIIYNEDGSPILEEVVFDNIIAEVEKHGSNIWYEKDAKYFLPEGYQNA
                     LSPNGNFTKEVDTMDVWFDSGISAIHTLKKHGLPFPADLYLEGNDQYRGWFNSSLTLS
                     VAALEKAPYKAVVTHGFVVDDKGEKMSKSKGNVLDPSKLANEYGADILRLWSATIDYQ
                     EDARLSVDLLRQTSETYRKIRNTFRFMLGNLPALDTSFAELKALYKEPDFYLDKLMTN
                     KLQQVINKMIDSYNDYDFGAAFTTLMNFVTNELSSFYLDVAKDILYCEAKDSARRQSV
                     VGTLYKITVALLPLIAPVLVFTAEEIYEHLPVIKRLPSVHLMDFPYQLDVDNAKLEEF
                     SALLKVRSNAYQHIEKARQEGVLGSSQEASLTLPHS"
     gene            complement(2774..3868)
                     /gene="ftsZ"
                     /locus_tag="PLQ55_02720"
     CDS             complement(2774..3868)
                     /gene="ftsZ"
                     /locus_tag="PLQ55_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPK28876.1"
                     /translation="MIGTNDLDNFEPVAKIVVIGVGGAGNNAVNRMIDDQIDSVSFWV
                     ANTDKQALATSKAKNRLVLGEEFTHGLGAGGEPTVGRKAAEASRPEIEKIVEGADLVF
                     IAAGMGGGTGTGASPIIAEIAKAAGALTIAIVTRPFTFEGKKRIANSVEGLNALKEIV
                     DSIIVVSNDKLLITGGNLPIAQAFSESDKVLAQSVKTVVDLILVPAVINLDFADVRNT
                     LLNSGIALIGFGMGKGKNKAHDAAQTAINSPLLEASLQGARKAIIAVTCGTNVSLFEA
                     QETVNEIVETSGSNLDVKFGVALNELLEDSIIVSVIASDFEEQYDFTSTHTSIIKPVT
                     PESRTYHAEDDEDDDSFLPNILKELEAEEN"
BASE COUNT         1100 a          881 c          698 g         1245 t
ORIGIN      
        1 cactatgtgg taatgttaag cttgcttctt gactactgcc aagtactcct tcttggcgcg
       61 ctttttcaat atgttgatag gcgttgcttc gtacttttag taacgcacta aattcttcaa
      121 gtttagcgtt atcgacatca agttgatagg ggaaatccat taaatgtacg gaaggtaaac
      181 gcttaatcac tggtaagtgc tcataaattt cttcagcggt aaacactaat acaggagcaa
      241 tcagtggtaa aagtgctacc gtaattttat aaagtgttcc cacaacactt tggcgtcgag
      301 cgctatcttt agcttcgcag tataagatat ctttcgccac atcaagatag aaactactta
      361 attcattcgt aacaaaattc atcagtgtag taaaagctgc cccaaagtcg taatcattat
      421 aactatcaat cattttatta atgacttgtt gtaatttatt agtcattaat ttatcaagat
      481 aaaagtctgg ttctttatat aatgctttaa gttcagcaaa acttgtgtct aacgcaggta
      541 aattaccaag cataaagcgg aaagtattac ggattttacg ataagtttca cttgtttggc
      601 gtaaaagatc aacactgagc cgtgcatctt cttgataatc aatcgtcgca ctccataagc
      661 gtaaaatatc cgcaccatat tcatttgcta atttacttgg atcaagcaca ttacctttgg
      721 atttagacat cttttcgcct ttatcatcaa caacaaagcc atgtgtcact acggctttgt
      781 atggtgcttt ttctaaggcc gcaacgctta aagtaagcga actattaaac caaccacgat
      841 attggtcatt accttctaaa tataaatcag cgggaaaagg taaaccgtgt tttttaagtg
      901 tatgaatcgc actgatgccg ctatcaaacc acacatccat cgtgtcaact tctttcgtaa
      961 agttaccgtt tggtgataat gcgttttggt acccttctgg taagaaatat ttcgcatctt
     1021 tttcgtacca tatattactg ccatgctttt ccacttccgc aataatgtta tcaaatacta
     1081 cttcttcaag aatcggacta ccatcttcat tataaataat cggaatgggg acaccccata
     1141 cgcgttgacg actaattgtc cagtcaccgc gatcactaat catattacta atccggcctt
     1201 caccccacgc tggataccag tcaacttttt ggacagcgtt aagtaaatct ctgcgaattg
     1261 gttcaatact ggcaaaccat tgcggtgttg cgcgaaagat aagcggttta tccgtccgcc
     1321 agtcgtgtgg gtaactgtgt tttactttcg tatgggcgac aagcgctcct acttcccgta
     1381 agtcattaat aataacttta ttagcgtctt cgtaaaatag tccttcatat ttcccagctt
     1441 cactagtaaa gtatcctcga gcgtcaacag gacataacac atctaagccg tacttttgcc
     1501 caaccacaaa gtcttcaagc ccgtgacccg gcgccgtgtg aaccgcccca gtcccgcttt
     1561 cactagtgac atgcatcccg gtgataacaa gggaaatacg ctcataaagc gggtgtttcg
     1621 tttcaattaa atctaaatcc gcacctttga cttcatattt taatgtaaaa gttaagtcac
     1681 tcgctttact tacttcttcg acaagttcag gtagtaaaat aacgtcacct aacgcgcttt
     1741 cgtaaacccc atatgtaaag gcgggatgga cactaatcgc taagttagca ggaagcgtcc
     1801 acggggttgt tgtccaaatc ataaatttag tgccaacagg tactttatca ttgccttttg
     1861 ttacattaaa aagcacataa attgagtcac taacgacatc tttatattca atttcggctt
     1921 cagcaagtgc agtttcacta cttggtgacc attgcacggg ctttagtccg cgataaatta
     1981 aacctttaag tgccatacgc ttaaagacac gaagttcatc agcctcgtaa tctttagtca
     2041 aagttaaata agcattttca aagtcaccga ctaagccaag acgcttaagt tgagcttttt
     2101 gaatttcgac ttgcttaagt gcatattctt tacataactt gcggaattct gtaattggaa
     2161 ttgctttacg gttaacccca ctttttgtga cttggttttc aattgggaga ccgtgcgtat
     2221 cccagccaaa aataaagggc gtgtcatagc cttgcatatt tttcatgcgt acgacaaagt
     2281 cttttaagtt acggttaagc atatgtccgc tatggatgtt cccgttagcg taaggtggcc
     2341 cgtcatggag aataaaaggt ttacttgttt catttttctt caccattaat ttatataaat
     2401 cgattgcctc ccaacgcgct aaaatacctg gttccttgcg ggttaagtta ccccgcattt
     2461 caaactttgt ctttggcatt aataatgtgt ctttaagttc catagtctct ctccttcaat
     2521 aaaaaaagtc tagtcacagg acgaattact ccgcggtacc acctgtgttc tagactaatc
     2581 tagcactttg tatctcgtaa cgtgagatta agcgacaact attactcttg ttcatagttg
     2641 cactcagaag tgataatggg agtagcttct tcttagggct tgcaccattt ccctagtcgc
     2701 tttgtaaggt tctaccgcca ccgtgtcttc gtccgatttc gctactatta tacattaaag
     2761 tataataatg tcattaattt tcttctgctt ctaactcttt taagatattt ggtaagaagc
     2821 tatcatcatc ttcatcgtct tcagcgtgat aagtacgcga ttctggtgtc actggtttaa
     2881 taattgaagt gtgcgtacta gtaaagtcat attgctcttc aaagtcacta gcaatgacac
     2941 tgacgataat ggagtcttca agtaattcat ttaatgcgac accaaattta acatcaagat
     3001 ttgatccgct tgtttcaacg atttcattaa ctgtttcttg tgcttcaaaa agtgaaacat
     3061 tcgtcccgca tgtgacagca ataattgctt ttcttgcccc ttggagacta gcttctaaaa
     3121 gcggactatt aattgctgtt tgtgctgcgt cgtgcgcttt atttttccct ttccccatcc
     3181 caaagccaat aagggcaata ccgctattta agagggtatt acgaacatcg gcaaagtcaa
     3241 gattaatcac agccggtact aaaattaagt cgacaactgt ttttacactt tgcgctaata
     3301 ctttatcact ttcactaaaa gcttgggcaa ttgggagatt tccaccagtg ataagtaatt
     3361 tatcattact aacaacgata atggagtcaa cgatttcttt taaggcatta agtccttcaa
     3421 cactattggc aatacgtttt ttaccttcaa aggtaaaggg ccgcgtaaca atcgcgattg
     3481 ttaaagcacc agcggctttc gcaatttcag caatgatggg ggaggcacct gtacctgttc
     3541 caccacccat acctgcagcg ataaagacaa ggtcagcacc ttcaacgatt ttttcaattt
     3601 cgggacgact agcttcggct gctttgcgac caacagttgg ttcgccaccg gcccccaaac
     3661 catgggtaaa ctcttcacca agcactaatc tatttttagc tttactcgtg gcaagtgctt
     3721 gtttatcggt gtttgccacc cagaaagaca cgctatcgat ttggtcatca atcatccggt
     3781 taaccgcgtt gtttccagcc ccaccaacac caataacgac tatcttcgct accggttcaa
     3841 aattgtctaa atcatttgtt ccaatcatac ttaactcctt gtcagtgtcc caatacttgt
     3901 cttctcatct ccaatatctt gcgc
//
LOCUS       DAOPCD010000080         3912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_18890_length_3912_cov_3.680840, whole genome shotgun sequence.
ACCESSION   DAOPCD010000080 DAOPCD010000000
VERSION     DAOPCD010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18890_length_3912_cov_3.680840"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2229)
                     /locus_tag="PLQ55_02725"
     CDS             complement(<1..2229)
                     /locus_tag="PLQ55_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28877.1"
                     /translation="MKTHKFKNFFSKVWISITVFFAFVLTIGYPVYAYFRANIERQVE
                     PTLEIELLFGHLREDLVTGEWGTDTNPYLIMSTDHLSNLSVLQNGAYSHYVNENSVFQ
                     VSDENGDPIYVGGATANQLFTIQSIGNEDYPFVSKIRGITTTDPNKYVTLPTGELTDT
                     SIIGNVRVTPEENQVDIGLFGCVGPDLEGEVPETDYQGEISNILLYNIQINTSTVGVM
                     PSGHYGYAKTGVTKETNHLGILAGHVQYANLDKISVYYSQTNGVAHVDAFNVSAGDTA
                     KYTTSGGIIGFYKRVIVDGEIENPVTSDGSDQGAGQAAAGLGKGIVYAEDIWKFMEEN
                     TTVGNPAPNNSYDLQDTFGDELYDPNDNSYFHVGVFTFAHSRQSRGKDRIAKIWTDAY
                     TNEWTISTNPTNPYTQTTRDVGPATKYTATRITNNEMSWNYTGWFNRQYYGTLNSPYS
                     SNADYRYMLVYEDTTVNPTKHYALVRYGSTAIGVEVDPNNLVIPDGEFKYFTYENLSQ
                     RTADITYPPYEAGFSYRFSGPITMDFRNTTSSAGTLQYAVYGDIVTGIVDGQTKILEA
                     PRPLRVNAQSNAVPTSSFMATASTSNPPYVEGYRISPTSNTGNFNKFYIQRTGASSSG
                     GSTSNWYMTYSTAEGFSAVSTSSPGTYASSFALYAVRYTNNTNNPSESPVATRNILEE
                     FIPAGTTETIDTEHNVLFYTGTYNSSVPTSRYSYEIKDISSLGWIDNRNKAITKGDTA
                     LLI"
     gene            complement(2216..2914)
                     /locus_tag="PLQ55_02730"
     CDS             complement(2216..2914)
                     /locus_tag="PLQ55_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28878.1"
                     /translation="MKEKKRNIVRTIWLMVLSLVASASLTYAWHISVYNFAQTHNVPG
                     ELTPEISLAHYNYTTSSWQNSTSAEPLSIYLGKMTNIAQLPANSNTYLKIRINETSDA
                     QTKYNVIVNNIETVISNIDGVHAVTGVDYFAAENAQNVFDFYVYTSTSDNLNPLTVFA
                     NLQTMTPFKITTLPHALLSNSATLDTWIYILLKPDIAKVQNIIRLVPIQLSPYNMVFR
                     MSLNGEVKTLDENA"
     gene            complement(2916..3659)
                     /locus_tag="PLQ55_02735"
     CDS             complement(2916..3659)
                     /locus_tag="PLQ55_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28879.1"
                     /translation="MDSQNKIKKVAKLPSRKFRLFLTFLGTAVSTVALTITTLAWFMS
                     TLKGGAEIPDIGVDDEYAHSFSCALNSVPITNGSVEFTPFYPGHVHRKTLTFSLTNLS
                     SKTFKMNLFFEQPAALEEVPYIDATGKWGNANYYYYLGSQIALTSVSLAIDGASPTFT
                     SGAGNHLVETNSVGVVKGQVNGVANELTSIARFYIIQNVSVPASKTLTGTLEFTFVDN
                     GTDQSMYFVDWPTTGVSRRSLSAFFYRGI"
     regulatory      complement(3709..3786)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT         1174 a          765 c          709 g         1264 t
ORIGIN      
        1 gattaatagt gcagtgtccc cttttgtaat tgctttattc cggttatcaa tccacccgag
       61 agaactaata tctttaattt cataactata gcgtgaggtc ggtacacttg aattataagt
      121 accggtgtaa aagagcacat tatgttcagt atctatcgtc tctgttgttc ctgctggaat
      181 aaattcttct aaaatattac gggttgccac tgggctttca cttggattat tcgtgttatt
      241 cgtgtaacgc acagcgtaaa gtgcaaagga agacgcgtaa gttcctgggc ttgaagtact
      301 aacagcgcta aagccttcgg cagttgaata agtcatatac cagtttgaag tacttccacc
      361 actactacta gcaccagtcc gttgaatata aaacttatta aaattacccg tatttgaagt
      421 aggactaatc cgataccctt caacataagg cgggttgctc gtagacgctg ttgccatgaa
      481 gcttgatgtt ggcactgcat tactttgcgc attaacccgt aatgggcgcg gtgcttctaa
      541 aattttagtt tgtccgtcca ctattccggt tacaatgtcg ccataaacgg cgtattgtaa
      601 agtccctgca cttgaagtag tgtttctaaa gtccattgtg atcggaccac taaaacgata
      661 agagaatcca gcttcataag gcggataagt aatatcagca gttctttgac ttaagttttc
      721 ataagtgaaa tatttaaatt caccgtccgg tataactagg ttattaggat ctacttccac
      781 accaatcgca gtagaaccat aacgcacaag tgcataatgc tttgtcggat taacagtcgt
      841 gtcttcatac acaagcatat aacggtaatc agcattacta ctatatggtg agtttagtgt
      901 tccgtaatac tgccgattga accatcccgt ataattccaa ctcatttcgt tattagtaat
      961 gcgtgttgcg gtatattttg tcgcaggacc gacgtcgcgc gttgtttgtg tatacggatt
     1021 tgttggatta gtggaaattg tccactcatt agtgtaagca tctgtccaaa tcttagcgat
     1081 acggtctttc ccgcgtgact gacgactgtg ggcaaaagta aatacaccaa cgtggaaata
     1141 tgaattatca tttggatcat aaagttcgtc accaaaagtg tcttgtaaat cataagaatt
     1201 attcggagca ggattgccaa cagtcgtatt ttcttccatg aatttccaaa tatcttcagc
     1261 gtaaacaatc cccttaccta aacccgctgc agcttgacca gcaccttgat cactaccgtc
     1321 actcgtcact gggttttcaa tttcaccgtc aacaataacg cgtttataaa aaccaataat
     1381 tccgccggaa gttgtatatt tagcggtgtc accagcgctg acattaaagg cgtcaacatg
     1441 ggcaacgcca tttgtttgtg aataataaac actgattttg tctaaatttg cgtattgaac
     1501 gtgacccgca aggattccta agtggtttgt ttctttagta acccccgtct tagcgtaacc
     1561 gtagtggccg cttggcatga caccaacggt gctagtgtta atttgtatgt tatataaaag
     1621 gatattagaa atttcccctt gatagtcggt ttcaggaact tcgccttcaa gatctggacc
     1681 aacacaacca aataacccaa tatcaacttg attttcttct ggtgtaacac gaacattacc
     1741 aataatacta gtgtcggtta attccccagt cggtaatgtc acatatttat ttggatcggt
     1801 tgtggtaatc cctctaattt tggatacgaa aggatagtct tcgttgccaa ttgattgaat
     1861 cgtaaataat tggttcgcgg ttgctccccc aacataaatt gggtccccgt tttcatcaga
     1921 aacttggaac acgctgtttt cattaacata atgactataa gcaccatttt gtaaaactga
     1981 aaggtttgat aagtgatcgg tgctcataat taaatatggg ttagtgtctg taccccattc
     2041 gccagttacg agatcttcac gtaaatgacc aaaaagtagt tcaatttcta gcgttggttc
     2101 tacttgtctt tcaatattag cgcggaaata agcatatact ggatagccaa ttgttaaaac
     2161 aaaagcaaag aaaacggtaa ttgaaatcca tactttactg aagaagtttt taaatttatg
     2221 cgttttcatc taatgtttta acctccccgt ttaatgacat tctaaacacc atattatatg
     2281 gtgataattg gattggaaca agccgaataa tattttgaac cttagcaata tctggtttta
     2341 acaaaatgta aatccatgta tctaaagtcg cactattgct aagtaatgcg tgtggcaaag
     2401 ttgtgatttt aaatggggtc attgtttgta agttcgcaaa tacagtcaag ggattaagat
     2461 tatcacttgt tgaagtatag acataaaagt caaagacatt ttgtgcattt tctgctgcga
     2521 aataatcaac accagtaact gcatgaacac catcaatgtt ggaaataact gtttcaatat
     2581 tgtttactat cacattgtat ttagtttgtg catcactagt ttcattgatt cttattttta
     2641 aataagtatt gctattagca ggaagctgtg cgatattcgt catcttccct aaataaatac
     2701 taagcggttc tgcactcgta ctattttgcc aactactcgt tgtgtaattg tagtgcgcta
     2761 gtgagatttc gggcgtaagt tcaccaggta cattatgtgt ttgtgcaaaa ttataaacgg
     2821 aaatatgcca cgcatatgtt aaagaagcgc ttgccacaag tgacagcacc attaaccaaa
     2881 tcgttctaac aatattgcgt ttcttttctt tcattttata ttcctcgata gaagaaagca
     2941 cttagcgatc ttcttgatac acctgttgtc ggccaatcaa caaaatacat actttggtca
     3001 gtgccattat ccacaaaagt gaattctaaa gtacctgtta aggttttact cgcaggcaca
     3061 gaaacatttt gaattatata aaagcgtgct attgatgtta attcatttgc gacaccatta
     3121 acttgtcctt taacaacacc aacactattt gtttccacta agtgattgcc tgcaccactt
     3181 gtgaaagttg gtgaagcacc gtcaattgcc agtgaaacag aagttaaagc aatttgacta
     3241 cctaaataat agtaataatt tgcattaccc catttacccg ttgcatcaat ataaggcact
     3301 tcttcaagcg ctgctggttg ttcaaaaaat aaattcattt taaaagtttt gctacttaaa
     3361 ttagtgagcg agaatgttaa tgttttacga tgaacatgac ccggataaaa tggtgtaaat
     3421 tcaacacttc cgttcgtaat aggaacgcta ttaagagcac aggaaaacga atgagcgtac
     3481 tcatcatcaa caccgatatc aggaatttca gcgccccctt taagcgtcga cataaaccat
     3541 gctagcgttg taatagtaag cgcgaccgtt gaaacagcgg tacctaaaaa tgtcaaaaat
     3601 agtctaaatt ttcgacttgg taatttcgct actttcttga tcttgttttg actatccatg
     3661 ttcactttct cttaatagcc ttttcctgta aaaaaagcta cctctctgtt aggtagctgg
     3721 ctatctcgtt tccaagatcc tatagctttg cgtcgctaga ttgctctagt tttgcctttt
     3781 tctagcatta ttatattcat aaatatgaat aatgtcaata atttttgtag atttatcggt
     3841 atttttctaa gaaataaaac ttaatcaact tcgttcatta ttcaaaacat taaaaaatat
     3901 cgttaatatg at
//
LOCUS       DAOPCD010000081         3879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_19075_length_3879_cov_3.399320, whole genome shotgun sequence.
ACCESSION   DAOPCD010000081 DAOPCD010000000
VERSION     DAOPCD010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19075_length_3879_cov_3.399320"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLQ55_02740"
     CDS             <1..184
                     /locus_tag="PLQ55_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004804231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28880.1"
                     /translation="LLDEPTSNVDSINEGIILRALKKHKEDRGIILVSHRESTMAIAD
                     RVYRFNGEIVEEVEDK"
     gene            185..631
                     /locus_tag="PLQ55_02745"
     CDS             185..631
                     /locus_tag="PLQ55_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HPK28881.1"
                     /translation="MKAKKIFWIILGCVSLALGAIGAALPILPTVPFLLLAAFSFAKS
                     SKRLHNWFINTKLYKKNLASYVKGKGMTWPAKIKMMITVTLVMLLGFIPMMIKKVYIP
                     SIILGVVWIFHIIWFIFGIKTYKKNSEDSLSTEVETPSETEEKNIE"
     gene            750..1472
                     /locus_tag="PLQ55_02750"
     CDS             750..1472
                     /locus_tag="PLQ55_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPK28882.1"
                     /translation="MKVENIYKQKYVLTTNYYDPSNNIGVQGTLDLFQNIAARHAYLL
                     GAGREHLEKINLVWVIARTEIEYVTYPETPLEVEVVTWPRPATRFYFDRYYKIVNPAT
                     NEIIAYSRSRWVLIDIDSRKIVGADRYDYPLDFYYEEEPFNHTFPRPSVSDNVVGNYT
                     VRPSDIDENGHLNNSKYGNIIYDYLDLQKGQKIKSIVINYNNEALQGETLTISRSRDD
                     AKVVISGHKGDTIVFSSEVNIQ"
     gene            1472..2293
                     /locus_tag="PLQ55_02755"
     CDS             1472..2293
                     /locus_tag="PLQ55_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPK28883.1"
                     /translation="MLTTLELWIIIIGMAIPFIATALGAAAVFFFRGPISERVNSILL
                     GFAAGVMIAASVWSLIMPSLEFAENSGWGKLAIIPVAVGFALGVLFLWFIDRIVPHMH
                     QRSEDEEGLPTKKLSLDSKLFLAMTIHNFPEGIAVGFAFAVAYTGIVNPGNASISLYS
                     ALALSIGMAIQNIPEGAAVSLPMARRLNNKGKAFGFGVASAIVEPIGAVLAFLFATVF
                     QTLLPWFLSFAAGAMMFVVVEDLIPEANLSGTEHQGTFAVMLGFIIMMIIDIVLG"
     gene            2299..2375
                     /locus_tag="PLQ55_02760"
     tRNA            2299..2375
                     /locus_tag="PLQ55_02760"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2333..2335,aa:Arg,seq:ccg)
     gene            complement(2433..3242)
                     /locus_tag="PLQ55_02765"
     CDS             complement(2433..3242)
                     /locus_tag="PLQ55_02765"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPK28884.1"
                     /translation="MNKPKDIKIIILLGQSNMEGHSWTSYLDRHFPPELVNYYTSPFK
                     TEIAYNCDNGNNESYDEFVPVRVGQGYDSTRFGPEIGIAAHLEKAQHSDEFKIIKFAI
                     GGTSLEVFWRSPSSGIPGLCYVFATNYAHRMLTKLKRDGYNPTVSAILYMQGENDSVD
                     GGHLQYEKYLQNLVNDLRNEFKDYAPKAGIYFIDGLINDAEFWTHHEIINTAKRNVAK
                     SDPKNIIVDTLTPQLSFSNEPDFEIDWAHYDSDGTIKLGHLFGEAVTEHIK"
     gene            complement(3248..3775)
                     /locus_tag="PLQ55_02770"
     CDS             complement(3248..3775)
                     /locus_tag="PLQ55_02770"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HPK28885.1"
                     /translation="MAKKRLLIVVDYQNDFVNGALGFSDAHEIEQKIIDTIEDHLKTK
                     DDIVFTKDSHFDNYLETEEGRNLPFKHCIKGTPGHDIHGKVAAYLPKAKKVYEKLTFG
                     SLELANDIVNKGYEEITLVGLVTNICILSNALLAKAALPEAKVIVLKDGVNSYDKDLH
                     AKTLDVLQGTQIIVK"
BASE COUNT         1202 a          655 c          765 g         1257 t
ORIGIN      
        1 attacttgat gagccaacta gtaatgttga tagtataaac gaaggcatta tcctaagagc
       61 attaaaaaaa cataaagaag atcgtggcat cattcttgta tcacaccgtg agtcaacaat
      121 ggcaatcgct gatcgtgtct atcgttttaa tggtgaaata gtagaggaag ttgaggataa
      181 ataaatgaaa gcaaaaaaga tattttggat tattttaggt tgtgtcagtc tcgcacttgg
      241 tgcaatcgga gcagcacttc ctattttacc gacggttcca tttcttttgc tcgccgcttt
      301 tagttttgcc aaaagttcga aacgtttaca taattggttt attaatacaa aactatataa
      361 aaagaatcta gcaagttatg ttaagggcaa aggcatgact tggccggcaa aaataaagat
      421 gatgattaca gtgacacttg tgatgttact tggatttatc ccgatgatga ttaaaaaagt
      481 atatattcct agcattattt taggagtcgt gtggattttt catatcattt ggtttatttt
      541 tggtattaaa acttataaga aaaatagcga agactcgctt tcaaccgaag tagaaacacc
      601 aagcgaaacc gaagaaaaaa acattgaata aaatttaaaa agtaagtaaa aaatataaat
      661 aaaatagtat aaatagcctt tattttcaca caaatggtgt aatattataa tgttgcctta
      721 atctaaagat taaaggagga agagcgacga tgaaagtaga aaatatttat aaacaaaaat
      781 atgtgctcac cactaattat tatgatccaa gtaataatat tggtgtacaa ggcacacttg
      841 atttattcca aaatattgcc gcccgtcacg cttatttatt aggtgccggt cgtgaacact
      901 tagaaaaaat taacttagtg tgggttatag cgcgcacaga aatagagtat gttacttatc
      961 ctgaaactcc gctagaagtt gaggttgtga cttggccacg tcctgcaaca aggttttatt
     1021 ttgatcgcta ttataaaatt gtaaacccag caacgaatga aataattgct tactccagaa
     1081 gtagatgggt tttaattgat attgattcgc gtaaaatagt aggcgctgat cgttatgatt
     1141 atccgcttga tttctattat gaagaagaac ctttcaatca caccttcccg cgacccagtg
     1201 ttagtgataa tgttgtaggt aattacaccg tacgcccaag tgatattgat gaaaacggac
     1261 acttaaataa ttcaaaatac ggaaatataa tctatgatta tcttgattta caaaaaggtc
     1321 aaaagattaa aagtattgta attaattata ataatgaagc tttacaaggt gaaaccttaa
     1381 caattagtcg cagcagggac gatgcaaaag tcgtaatttc tgggcataaa ggtgatacaa
     1441 ttgtgttcag tagcgaggtc aatattcagt aatgttaaca acacttgaac tatggattat
     1501 tattatcggg atggctatac cattcattgc gactgcgctt ggtgcagcag cggtattctt
     1561 tttccgcggg cccataagtg agcgagttaa tagtatttta ctaggatttg ctgcgggtgt
     1621 aatgattgct gcttcagtgt ggtcattaat aatgccaagt ttagaatttg ctgaaaattc
     1681 aggctggggt aaattagcga ttattccagt tgcggtcgga tttgcattag gagtactctt
     1741 tttatggttt attgaccgta ttgtccctca tatgcatcaa cgtagtgaag atgaagaagg
     1801 attaccaact aaaaaactaa gtcttgattc aaaattattt ttagcaatga caattcataa
     1861 ctttcctgaa ggtattgcgg tcggctttgc ttttgctgtg gcttatacag gtattgttaa
     1921 tcctggaaat gcttctattt cgctctatag tgccctagcc ctctcaattg ggatggcaat
     1981 tcaaaatatt cctgaaggag cggctgttag tttaccgatg gcgcggcgtc ttaataataa
     2041 aggtaaagca ttcggatttg gtgttgctag tgcaattgtt gaaccaattg gtgcagtttt
     2101 ggcgtttcta tttgccacgg tgtttcaaac actcttaccg tggtttttaa gttttgccgc
     2161 aggagcgatg atgtttgttg ttgtggaaga tttaattcct gaagcaaatt taagtggaac
     2221 tgagcaccaa ggcacatttg ctgtaatgtt aggctttatt attatgatga ttatagatat
     2281 agtattaggg taataaatgc gctcgtagct caggtggata gagtacaacc ctccgaaggt
     2341 tggggtcaca cgttcgagtc gtgtcgggcg cgccataaag aaagagaaaa gcacccagtc
     2401 gcgggtgctt ttttgtttat tgttattagt aactatttaa tatgttcagt aacagcttca
     2461 ccaaataagt gaccaagttt aatagtacca tcactatcat aatgtgccca gtctatttca
     2521 aaatccggtt cattgctaaa agaaagttgt ggggtaagtg tatcgacaat gatgtttttt
     2581 ggatcacttt ttgctacatt acgcttagcg gtattaatta tttcgtgatg tgtccaaaat
     2641 tcagcatcat taataaggcc gtcaataaag taaatgcctg ctttaggtgc gtaatcttta
     2701 aattcgttcc gtaaatcatt aacaagattt tgtaaatact tttcgtattg aagatgccca
     2761 ccatcaactg aatcattttc accttgcata taaagaatag cgctgacagt tggattatag
     2821 ccatcacgct ttaattttgt aagcatacgg tgcgcataat tagtggcaaa cacataacat
     2881 agtccaggga tcccacttga tggtgagcgc caaaatactt caaggctagt gccaccaatc
     2941 gcgaacttaa taattttaaa ttcatcactg tgctgagctt tttctaagtg cgcagcaatc
     3001 ccaatttcag gaccaaaacg cgtagagtca tatccttgtc caactctgac agggacaaat
     3061 tcatcatatg attcattatt accattatca cagttatagg caatttccgt tttgaaaggg
     3121 gaagtgtagt aattaactaa ttcaggcggg aaatgccgat ctaaataact agtccacgaa
     3181 tggccttcca tatttgattg gcctaaaaga atgatgattt taatatcttt aggtttgttc
     3241 ataaagttta tttaacgata atttgtgtgc cttgtaacac atctagtgtc ttagcgtgta
     3301 agtctttgtc atagctattg acaccgtctt taaggacgat aacttttgct tcaggtaaag
     3361 cggctttagc aagtaaagca ttacttaaaa tacaaatatt tgtcactaaa ccaacaagcg
     3421 taatttcttc ataaccttta tttacaatat cgttagcgag ttcaagtgaa ccaaaagtta
     3481 acttttcata aactttcttt gcttttggta agtaggctgc tactttacca tgaatatcat
     3541 gacccggtgt acctttgata cagtgtttga aaggtaaatt acgcccttct tcagtttcta
     3601 aatagttatc aaaatggctg tctttagtga agacaatgtc atcttttgtt tttaagtggt
     3661 cttcaatagt atcaatgatt ttttgttcga tttcatgggc atcactaaag ccaagtgctc
     3721 cattaacgaa gtcattttga tagtccacaa caattaataa acgttttttc gccatttgtt
     3781 ttcctccttc atggcggaga aagagggatt cgaaccctcg cgaagattgc tcctcctatc
     3841 ggttttcgag accgacccct tcagccaggc ttgggtatt
//
LOCUS       DAOPCD010000082         3822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_19410_length_3822_cov_4.121317, whole genome shotgun sequence.
ACCESSION   DAOPCD010000082 DAOPCD010000000
VERSION     DAOPCD010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19410_length_3822_cov_4.121317"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PLQ55_02775"
     CDS             <1..570
                     /locus_tag="PLQ55_02775"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPK28886.1"
                     /translation="RVFLYDENYELITFDDHHTLPSSWTDWKTLRSFHTPKAVSKIAL
                     VPIGSRVTPPYVHELFTYSQDALESIRQTSITNSLTDVKVTTNTATFKTNYPTRKFVV
                     TTIPYDKGWRVTNENNIKIPVYRVQGGFVGFVSDRGEMSYTMSFRPQYFNMGLVLTFS
                     AGIATLLLELNVYFFKKYKKKKAEENINV"
     gene            complement(560..1402)
                     /gene="rsmI"
                     /locus_tag="PLQ55_02780"
     CDS             complement(560..1402)
                     /gene="rsmI"
                     /locus_tag="PLQ55_02780"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPK28887.1"
                     /translation="MKRNKSFSGTPFYLVATPIGNLEEFSPRAVNVLRSVDFICAEDT
                     RTSGVLLNHFGIKKELISVHKFNEQSKLELILNKLKNGEKGAFISDAGYPLISDPGHP
                     LVKALIANDFSVSVINGPSALLPALIGSALDTETFTFIGFLKGNNKDVLNTLKRYKSY
                     KDTLIIYEAPHRVVKTIATIYTVFAERKLVIARELSKLHEEYIYTSLAEFSANPPTDL
                     KGEIVLVIEGNKDEENNDLADLLDKVELLISNENMSVKKATSVVALLYNVSKNKLYNA
                     YLKR"
     gene            complement(1389..2222)
                     /gene="ricT"
                     /locus_tag="PLQ55_02785"
     CDS             complement(1389..2222)
                     /gene="ricT"
                     /locus_tag="PLQ55_02785"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPK28888.1"
                     /translation="MNDNNERYFISIIYNDGGKSYCFGTNESSLDIGDYVLVESSQGI
                     AVAKVVATPQTMDKYSSPLELKDIVRRANEHEIRQHQDNLKDAELAMAFCQKQVERLG
                     LKMNLLTSEYTFDRSKINITYSAEERVDFRELVKILGSHLKTRVELRQIGSRDRAKLI
                     GGLGQCGFPLCCHTFLTEFDGISINRAKNQMLPLNIPKLSGHCGKLLCCLKYEDDYYT
                     EAKKEFPNVGSTYTRGNTLYQLTSFNIVNRTLRLDYEDGFVIVPLDELKRDYKVKHEK
                     K"
     gene            complement(2215..3201)
                     /locus_tag="PLQ55_02790"
     CDS             complement(2215..3201)
                     /locus_tag="PLQ55_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28889.1"
                     /translation="MTIYNYLKNYQPILFQTFGNALTTNNLSHAYLLDGEPGTPLKEV
                     AFYLAKSLVCDDPNPFACDKCLACHRFEEGNYTDFYFFDGSESSIKKDSVLELEKAFS
                     MTSVEHKGVLIYVIHNVENMTIEAINSLLKFLEEPAGKVYAFLTTNNLHNVIPTIKSR
                     SQILSLQLIKRDVLLKLCESIALPQDDIEMLVPFYNIPSLIEEEAKSERYPEYKRAVI
                     DTLETFSDNPKRGYLKAHELIVPLRERFVIKRVFTYFALFFKDVVNYNIGHERLFKSY
                     DPLIKKLAATIKNPLPLIVATYEISKKVDDYVNTGLLVDELLIKMVKETTDE"
     gene            complement(3198..3821)
                     /gene="tmk"
                     /locus_tag="PLQ55_02795"
     CDS             complement(3198..3821)
                     /gene="tmk"
                     /locus_tag="PLQ55_02795"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPK28890.1"
                     /translation="MFITFEGPEGSGKTSIANRIIKTLIDKGYDIVHTREPGGTPIAE
                     QIRDVTLDKKNTALDARAEALLIAASRRQHVVEKIRPALAEGKIVICDRYLDSSLAYQ
                     GAARGLGIDTVLAINLFAVEDTLPNLTLLFDLEPAIGFERIKANKNREVNRLDLEERN
                     FYIKVRNAYLELSKKYKDRYRIIDASQSFEKVYEDTLKVILEYLPQK"
BASE COUNT         1213 a          715 c          612 g         1282 t
ORIGIN      
        1 cgggtgttct tatatgatga aaactatgaa ttaattactt ttgatgatca tcatacttta
       61 ccgagttcat ggacagattg gaaaacttta cggagtttcc acacaccaaa agcggtatct
      121 aaaattgcgc tagtgccaat tggcagtcgt gttactccgc catatgttca tgaattattt
      181 acctattcac aagatgcact agaatctata agacaaacat caatcactaa ttcactaact
      241 gatgttaagg tcacaacgaa taccgcgaca tttaaaacta attatccgac gcgcaagttt
      301 gttgttacca caattcctta cgataaggga tggcgtgtca ctaatgaaaa caatataaaa
      361 atacccgttt atcgggtaca aggtggattt gttggttttg ttagcgatcg gggtgaaatg
      421 agttatacaa tgtcgtttag accgcaatac tttaatatgg gtttagtgct tactttctct
      481 gcaggtattg ctacattatt actagaacta aatgtgtact tctttaagaa atataagaag
      541 aaaaaagccg aagaaaatat taacgtttaa gataagcgtt atatagttta tttttagaaa
      601 cattatagag caaggcgacg acactagtcg cctttttaac actcatattt tcattactaa
      661 ttaatagttc tactttatct aaaaggtccg ctaagtcatt attttcttca tctttattgc
      721 cttcgatgac taaaacgatt tcacctttta aatccgtcgg tggattagca ctaaattccg
      781 ctagtgatgt ataaatatat tcttcatgca atttacttaa ttcacgcgca attacaagct
      841 ttctttcggc aaaaaccgtg taaatcgtcg ctattgtctt aacaacacga tgcggagctt
      901 cgtaaattat taatgtgtct ttgtaagatt tataacgctt taaagtgttg agcacatctt
      961 tattattacc ttttaaaaag ccgatgaatg taaatgtttc agtatctaag gcgctaccaa
     1021 tcagcgctgg taaaagtgca ctaggaccat taataacact aacactaaaa tcgttagcga
     1081 ttaacgcttt aactaacggg tgtcctggat cactaataag cggataaccg gcatcactaa
     1141 taaaggcgcc tttttctccg ttttttaatt tatttaagat taactctaat ttactttgct
     1201 cattaaattt atggacacta ataagttcct ttttaatacc gaaatgattt aaaagaacac
     1261 cgcttgttct ggtatcttca gcgcaaataa aatcaacgct tcttagcaca ttaacagcgc
     1321 gcggcgaaaa ctcttctaaa ttgccaattg gcgtagccac taaataaaat ggtgtaccgc
     1381 taaaagattt atttcttttc atgtttaact ttataatccc tttttaattc atctaagggc
     1441 acaatcacaa aaccatcttc ataatcaaga cgaagagtac ggttaacgat attaaatgat
     1501 gttagttgat acaaagtgtt accgcgagtg taagtcgaac caacattagg gaattctttt
     1561 tttgcttccg tataataatc atcttcatac tttaaacaac aaagtaattt accacagtgg
     1621 ccacttaatt ttggaatgtt aagcggtaac atttggtttt tagcacgatt aatcgaaata
     1681 ccatcaaact ctgttaagaa agtatggcaa caaagcggga agccgcattg cccaagacca
     1741 ccaattagtt tagcgcggtc tctactacca atttggcgta gttctactct agtttttaag
     1801 tgtgaaccaa gaattttcac aagctctcta aagtcaacgc gttcttcagc ggagtaagta
     1861 atattaatct tactacgatc aaaagtatat tccgaagtaa gtaaattcat tttaagacca
     1921 agccgttcga cttgcttttg acaaaaggcc attgcaagtt cagcatcttt tagattatct
     1981 tgatgttgac ggatttcatg ctcattagcg cgtctaacaa tatcctttaa ctctaatggg
     2041 ctactatact tatccattgt ttggggcgtt gccactactt tagcaacggc tataccttga
     2101 ctgctttcta ctaaaacata atcgcctata tctaaagaac tttcatttgt tccaaaacaa
     2161 taagacttgc caccatcgtt atatataata cttataaagt aacgttcatt gttatcattc
     2221 atctgttgtt tcctttacca ttttgattaa taattcatcc acgagtaagc cagtattaac
     2281 ataatcatca acctttttac ttatttcata agtagcaaca attaatggca gtggattctt
     2341 tatcgtcgct gctaactttt taataagcgg atcataactc ttaaaaagtc gctcgtgacc
     2401 tatattatag tttacaacat ctttgaagaa aagggcaaaa tacgtaaata cacgcttaat
     2461 tacaaagcgt tcacgcagcg gaacgattaa ttcgtgggcc tttaagtagc cgcgcttagg
     2521 attgtcgcta aaagtctcta aagtatcaat tacagctctt ttatactctg gatatcgttc
     2581 tgattttgct tcttcttcaa ttaaactagg aatattataa aaaggcacaa gcatttctat
     2641 atcatcttgc ggtaacgcta ttgattcgca aagttttaag agtacatcgc gtttaattag
     2701 ttgtaatgaa agtatttgac ttcgactctt aatagtcggg ataacattat gtaagttatt
     2761 tgtagttaaa aaagcatata cttttcccgc tggctcttcg agaaacttta aaagtgaatt
     2821 aattgcttca atagtcatat tttcaacatt atgaatcaca taaattaaaa cacctttatg
     2881 ttcaacacta gtcatgctga aggctttttc aagttctaat acactatctt ttttaatact
     2941 tgattctgag ccgtcaaaaa aatagaaatc agtgtaatta ccttcttcaa aacgatgaca
     3001 cgctaagcat ttatcacacg cgaaaggatt aggatcatca cacactaaac tttttgctaa
     3061 ataaaaagca acttctttaa gtggtgttcc aggttcacca tcaagcaaat aagcgtgact
     3121 taaattattt gtagttaaag catttccaaa tgtttggaat aaaataggct gataattttt
     3181 taaataatta taaattgtca tttttgtggg agatattcta aaataacttt tagtgtatcc
     3241 tcataaactt tctcaaaact ttggcttgcg tcaattattc gataacggtc tttatatttt
     3301 ttacttagtt caaggtaggc attgcgcacc ttaatgtaaa aattgcgttc ttctaagtct
     3361 aggcgattaa cttcacgatt tttattagct ttaattcgtt cgaaaccaat tgctggctct
     3421 aaatcaaata aaagtgtcaa atttggcaag gtgtcttcaa ctgcaaaaag gttaattgcc
     3481 aggactgtgt cgattcctaa acccctagcc gctccttgat aggcgagcga agaatctaaa
     3541 taacggtcac aaataacaat ttttccctcg gctagtgctg gacgaatttt ctcaacgaca
     3601 tgttgccttc tacttgctgc aattagaagt gcttctgccc gagcatcaag cgccgtgttt
     3661 ttcttgtcta aagttacatc acgaatttgt tcggcaatag gagtgccacc aggttcacgt
     3721 gtatgaacaa tatcataacc tttatcaata agtgttttta taattctatt agcaatacta
     3781 gtttttccgc ttccttctgg tccttcaaat gtaataaaca ta
//
LOCUS       DAOPCD010000083         3802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_19543_length_3802_cov_3.353349, whole genome shotgun sequence.
ACCESSION   DAOPCD010000083 DAOPCD010000000
VERSION     DAOPCD010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19543_length_3802_cov_3.353349"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PLQ55_02800"
     CDS             complement(<1..600)
                     /locus_tag="PLQ55_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPK28891.1"
                     /translation="MTIFIRDNYNEMSKAAAKIIAKQIKEKPDSVIGLATGFTPIGTY
                     RELIRMHKEEGLSFKGVTAFTLYEFLGEGFDIIKPYGLDKSSARYMHEEFFKHVDIDK
                     KNIHYFDGFTDDPRRTCQEYEEKIKAAGGIDLQLLGIGRDGHWGYNEPGSSLGSRSHV
                     VALTKQTIDDNYEDFYRKVGITKDEMPHFALTMGVGTILD"
     gene            720..1649
                     /locus_tag="PLQ55_02805"
     CDS             720..1649
                     /locus_tag="PLQ55_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPK28892.1"
                     /translation="MNILMILTPKSEVIYLSTKMRVRDAMEVLKKARFNSVPLLDESG
                     HYVGTVTEGDILWHLDQNGYEDSLDMRLKNVKRIRDYAPVSVDEEIDELIHKALGQNF
                     IPILDDRKYFIGIVTRKNLISTYIKKVDDNKEEVVHKNEVLDNLYKRRSIRRFKEGIT
                     IPQEIIDEIVKVSLVTPTAMNRRPHEIMLVDDPQVIKTLSELHQRGGQFEKAPYLILI
                     LNDDEKEPNAYLANSNCSALLISLLLAVESFDNLGGFWVSTRYPEYNRKMLNAVGVPE
                     RFSLYGMVAFGVKGEHKPANEPLNPEKIHRNKW"
     gene            1720..2274
                     /locus_tag="PLQ55_02810"
     CDS             1720..2274
                     /locus_tag="PLQ55_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPK28893.1"
                     /translation="MAKNIMVVTGSVRKTGNSIMLANAFVKGARNNGNAIQKVEASHR
                     NFSPFRDKENAWRKGKPSLVEDDFNVLAPYLHVSDVLVLASPVYFGGFSGAIKVFIDN
                     LYAYVVEHRKRNLRFTHAILLAVARDDNPDTFVGITHEFKELCRQLGIEKMDIITVPG
                     VAERGDIVGHEALDRARDLGKTIS"
     gene            2286..3287
                     /gene="galE"
                     /locus_tag="PLQ55_02815"
     CDS             2286..3287
                     /gene="galE"
                     /locus_tag="PLQ55_02815"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPK28894.1"
                     /translation="MKILVTGGTGFIGSHTVVALLEEGHEVVIADNLVNSQRNVVTKI
                     KRITGVKPFFYKVDVADKKSMRKVFSEHKFDAVIHFAGLKAVGESVQKPLLYYRNNLD
                     STLTLLELMKEFDVKNILFSSSATVYGTPENVPLKEEDPIGGTTNPYGTTKLIIEQIL
                     MDFVHANEGFKAVSLRYFNPIGAHPSKLLGESPKDIPNNLMPYISRVANGKLSHLNVF
                     GDDYETPDGTGIRDYVHVLDLANGHVLALKAFSFNHAYNVYNLGTGKGTSVLELVHLY
                     EKANKLKIPYVIGPRREGDIAASYADITKAKNELGFVPHYSVKDACRHAYLFEKKQK"
     gene            3405..>3802
                     /locus_tag="PLQ55_02820"
     CDS             3405..>3802
                     /locus_tag="PLQ55_02820"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPK28895.1"
                     /translation="MENYSHKYKISPELTLYGAYWGVKKPRANVLVITGMQEHAMRYT
                     EFAKFLNEAEFSVYSIDYFGQGENVTQGGHTLGVLPNHGFEKFVTALGTFVENLAKDK
                     LPLYVLGHSMGSFLAQRFVQRYPLLSKKVV"
BASE COUNT         1249 a          635 c          737 g         1181 t
ORIGIN      
        1 atctaaaatt gtgccaacgc ccattgttaa ggcaaagtgc ggcatttcat ctttagtaat
       61 tcctactttc cgataaaagt cttcatagtt atcatcaatt gtttgtttag ttaaagcaac
      121 aacatggcta cgactaccta aacttgagcc tggttcgtta taaccccagt ggccatcacg
      181 gccgatacct aagagttgta agtcaattcc gcctgctgct ttaatttttt cttcatattc
      241 ttgacaggtg cgacgtggat catcggtaaa tccgtcaaaa taatggatat ttttcttatc
      301 gatgtcgaca tgtttgaaaa attcttcatg catgtaacga gcgcttgatt tatctaatcc
      361 gtaaggctta ataatatcaa aaccttcacc taagaattca taaagggtaa aagctgttac
      421 tcctttaaaa cttaaacctt cttctttatg catccgaatt agttcacgat aagtgccaat
      481 tggtgtaaag cctgtcgcaa ggccaataac tgaatcaggt ttttctttaa tttgcttagc
      541 aattattttt gctgctgctt ttgacatctc gttataattg tcacgtataa aaatagtcat
      601 aatattctcc tcataaatag tctaacatat agttacacca tataatacaa caaagttcct
      661 ttttttacca cagtgaaagc ggttttatta tataattata gtatagaaag aggtggagca
      721 tgaacatttt aatgatacta accccgaaga gtgaagtcat atatttatca actaaaatgc
      781 gtgtaagaga tgcgatggaa gtgttaaaga aagcacgatt taatagtgta cctttacttg
      841 atgaaagtgg tcattatgtt ggcactgtca ctgaaggtga catcttatgg catcttgatc
      901 aaaatggtta tgaagattca cttgatatgc gcttaaaaaa cgttaaacgc attcgggatt
      961 acgctcctgt atcagtggac gaagagattg atgaattaat tcataaagca ctcggtcaaa
     1021 actttattcc aattcttgat gatcgaaagt actttattgg tattgtgaca cggaagaact
     1081 taattagcac ttatattaaa aaggttgatg ataataaaga agaagttgtt cataaaaatg
     1141 aagtgctcga caatttatat aaaagaagaa gtattagacg ctttaaagaa ggcataacta
     1201 ttccacaaga aataatcgat gaaattgtta aagttagttt agtaacccca acggcaatga
     1261 atcgacgccc acatgaaata atgttagtcg atgatccaca agttatcaaa acacttagcg
     1321 aattacatca aagaggcggc caatttgaaa aagctccata tttaatatta atattaaatg
     1381 atgatgaaaa agaaccaaat gcttatcttg caaatagtaa ctgcagtgct ttacttatta
     1441 gcttattact cgctgttgaa agttttgata atttaggtgg gttctgggtt agtacccgtt
     1501 atccagaata taacaggaag atgctaaacg ctgttggtgt accagaacgc tttagtctct
     1561 acgggatggt tgcctttgga gtcaagggtg aacataaacc ggcaaatgaa ccacttaacc
     1621 cagaaaaaat tcatcgtaac aagtggtaaa aacttaataa aaggcacgat tttaagcaca
     1681 atataacaat tttatgatat aattatttat ggaggcaata tggcaaaaaa cattatggta
     1741 gttacaggca gcgtccggaa aacaggcaac tcaattatgc ttgcaaatgc ttttgttaaa
     1801 ggcgcgcgaa ataatggtaa tgcgattcaa aaggttgaag cttcgcatcg taatttttca
     1861 ccatttcgcg ataaagaaaa tgcatggcgg aaaggtaaac caagtttagt cgaagacgat
     1921 tttaatgtcc ttgccccata tttacatgtc agtgatgttt tagtgcttgc ttcacctgtt
     1981 tactttggtg gctttagcgg cgcaatcaaa gtattcattg ataatcttta tgcttatgtt
     2041 gtcgaacatc gtaaacgcaa cttacgcttt acgcatgcaa tactccttgc ggtagcgcgt
     2101 gacgataatc ccgatacatt tgttggtatt acccatgaat ttaaagaatt atgtcggcag
     2161 ttaggtattg aaaaaatgga tattattact gtacctggtg tagctgaacg tggtgatatt
     2221 gttgggcatg aagcacttga ccgtgcgcgg gatttaggaa aaacaattag ttaggaaata
     2281 gttttatgaa aatattagtt actggaggca ctggcttcat cggttcacat accgttgtgg
     2341 cactactaga agaaggtcat gaagtcgtta ttgctgataa tttagtgaac tcacaaagaa
     2401 atgtcgtcac taaaattaaa cggattacag gagtaaagcc gtttttttat aaagtcgatg
     2461 ttgctgataa aaaaagcatg cgaaaggttt tcagcgaaca taaatttgac gctgtgattc
     2521 attttgctgg gcttaaagcg gttggtgaaa gtgtgcaaaa accgctcctt tattacagaa
     2581 acaacttaga ttcaacatta acactgctgg aactgatgaa agaatttgat gtgaaaaaca
     2641 ttcttttttc cagtagtgca acggtatatg gcacccctga aaacgtcccg cttaaagaag
     2701 aagacccaat cggcggtact accaatccct atggcactac aaagttaatt attgagcaaa
     2761 tcttaatgga ttttgtgcac gctaatgaag gttttaaagc agttagttta cgctatttta
     2821 atccaattgg tgctcatcct tcaaaattac ttggtgaatc accaaaggat attcccaata
     2881 acttaatgcc ttacattagc cgtgtcgcta acgggaaatt aagtcactta aatgtgtttg
     2941 gtgatgatta tgagacgcct gatgggacag gaattagaga ttatgtccat gtccttgatt
     3001 tagctaatgg tcatgtttta gcgcttaaag cctttagttt caatcatgct tataatgttt
     3061 ataacttagg gacaggcaaa ggcactagcg tcctcgaatt agtgcatctt tatgaaaaag
     3121 caaataaact taaaattccg tatgtcattg gtccacgtcg tgagggtgat attgccgcaa
     3181 gctacgctga tattacaaaa gctaaaaatg aattaggctt tgttccgcat tattcagtaa
     3241 aagatgcttg tcgccacgct tatctatttg agaaaaaaca aaagtaataa tttttatatt
     3301 tatatcaaaa aattaaggag taatggttaa ccataaatat tttcatttta cttatgtaag
     3361 caatatgtaa ataaatggta aaattaaaag aaggaggacg taatatggaa aattacagcc
     3421 acaaatataa aatctccccc gaacttacac tttatggtgc ttattggggt gttaaaaaac
     3481 cgcgggcgaa tgtcttagtc atcacaggga tgcaagaaca tgcaatgcgc tacactgaat
     3541 ttgcaaaatt cttaaatgaa gcggaattta gtgtatatag tatcgactat tttggacaag
     3601 gagaaaatgt cacgcaaggt ggtcatactt taggtgtttt acctaatcac ggctttgaaa
     3661 aatttgtgac ggcgcttggt acttttgtag aaaacctagc aaaagataaa ttaccacttt
     3721 atgttttagg gcattcaatg ggtagtttct tagcgcaacg cttcgtgcaa cgttatccgc
     3781 ttctttctaa gaaagtggta at
//
LOCUS       DAOPCD010000084         3744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_19909_length_3744_cov_3.213608, whole genome shotgun sequence.
ACCESSION   DAOPCD010000084 DAOPCD010000000
VERSION     DAOPCD010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19909_length_3744_cov_3.213608"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..564
                     /gene="rpsO"
                     /locus_tag="PLQ55_02825"
     CDS             295..564
                     /gene="rpsO"
                     /locus_tag="PLQ55_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPK28896.1"
                     /translation="MALTKERIAEIVKEFGANEKDTGSTPVQVALLTEQIRELTEHMK
                     VHKHDYHSQRGLLKLVGQRRGLLDYYNRVNREGYLALIAKLGLRR"
     gene            625..2787
                     /gene="pnp"
                     /locus_tag="PLQ55_02830"
     CDS             625..2787
                     /gene="pnp"
                     /locus_tag="PLQ55_02830"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPK28897.1"
                     /translation="MKKVYEMMFHGRKIVVETGEFAKQADGAVLVRYGDTAVLSTAVA
                     GNNPTNLDYFPLSVAYQEKMYAAGKIPGSFLRREGRETEHATLIGRLIDRPIRPLFAE
                     GFRNEVQVINTVLSADYDCTPEMTAMFGSSLALTISDIPFEGPIAGVQVGRVDGKFII
                     NPTTEENAKSDLQLTVAGTKRAINMVEAGANEVSEEDMLEALMLGHEAIKELCAFQEE
                     IQRDIGVAKREFPLFVPDESLVAELKEKYTGKLTEAISVFDKLERSAAIEAVKGEMVE
                     EYEAKEYESDDDKRRVISDVKALHEVMVSDEIRRLITHEKVRPDGRKVDEIRPLDSQV
                     DLLPRTHGSALFTRGQTQVLSVVTLGPKSDEQIIDNLTDEESRRFMHHYNFPPYSVGE
                     IKRLGGPARREIGHGALGERALLAVIPSDEEFPYTIRVVSEVLESNGSSSQASICASS
                     MALMAAGVPIKDMVTGIAMGLITSGPLDGNHPYTILTDIQGLEDHFGDMDFKVAGTSK
                     GITALQMDIKVRGISKEVLAESLAQAKKAREEIRANQLSAISAPREDVAEFAPKMGKM
                     TIDVDKIREVIGTGGKVINKIIEDNDGAKIDIDDDGLVVVYHMNRATINNVLATIEAI
                     VKEAKAGEIYEGKVTRVEPYGVFVNLFGNVDALVHVSDLDWAYVDDASKLYKIGDTLK
                     VKVTGVDEKGKVKASVKVFKAKPEGYTERKSGGRRNNK"
     gene            2946..>3744
                     /locus_tag="PLQ55_02835"
     CDS             2946..>3744
                     /locus_tag="PLQ55_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPK28898.1"
                     /translation="MYDIIIIGAGVIGALIGREASKYELNILMLEKDNDVGNGTSAAN
                     SAIVHSGYDPKPGTLKAKFNVASVPLYKDLCSDLDVEFKRIGSLVVAQGEEELPRLDE
                     LKARAELNGVEVKMLNKAEVHALEPNLHDHIVGGLFAPTAGIVNPFELTAHAIENAIE
                     NGMTLKLNEEVVAIKKLEDGFKVTTKNGDYFSKVVINAAGVFADDIINMVSKADFEIT
                     PRKGSYFVLDKLTVPLVNTVLFPLPNIHSKGILVVPTTAGNTLIGPTS"
BASE COUNT         1233 a          595 c          849 g         1067 t
ORIGIN      
        1 gaagcggtgc gtaattatat gaaccgcgca agtgaattac gctttaagaa agttgatgaa
       61 ggctttaaag tgcgcttaaa acctagtgaa aaagaattag aagatactcg actctacgct
      121 cgtaatttca tcatgaaaat aaaagcagag ttaaataaat aattaagcat atttaacttg
      181 gcaatttacc aagtaatatg ttaatattat gtagcgactt tagcgctgct atgattcacc
      241 cctagaatta gggcgatgct aaagttaaga ataaagagaa aggggaataa agttatggca
      301 ttaacaaaag aacgcattgc cgaaatcgtt aaagaattcg gtgcgaatga aaaagataca
      361 ggatcaactc ctgtgcaagt ggcgttatta actgaacaaa ttcgcgaatt aactgaacat
      421 atgaaagtcc acaaacacga ctatcattca caacgcggat tacttaaatt agttggtcaa
      481 cgtcgtggtc ttcttgatta ctataaccgt gttaatcgtg aaggttactt agctttaatt
      541 gcaaaattag gcttacgtcg ttaaaaaaga ttaaactgtc tatgacagtt ttttcattat
      601 ttatgttgga gaaaagagga aaagatgaaa aaagtttatg aaatgatgtt ccatggccgt
      661 aaaattgtcg tggaaactgg cgaattcgcc aaacaagctg atggcgctgt gttagtgcgc
      721 tatggtgata cggcagtctt aagtactgct gttgcgggaa ataatccgac aaatttagat
      781 tactttccac tctcagttgc gtatcaagaa aaaatgtatg cagcgggaaa aattcctggt
      841 tcatttttac gtcgtgaagg acgtgaaaca gaacacgcta ctttaatcgg tcgtttaatt
      901 gaccgtccga ttcgtccgtt attcgcggaa ggatttagaa atgaagtaca agttattaat
      961 actgtcttaa gtgccgatta cgactgcact ccagaaatga cagcaatgtt tggtagcagt
     1021 ttagcgctta cgattagtga tattccgttt gaaggaccaa ttgctggtgt gcaagttggt
     1081 cgcgttgatg gtaaatttat tattaaccca acgaccgaag aaaacgctaa aagtgactta
     1141 caactgacag ttgccggtac aaagcgtgct attaacatgg tcgaagctgg cgctaatgaa
     1201 gttagcgaag aagatatgct tgaagcctta atgcttgggc acgaagcaat taaagaactt
     1261 tgtgcattcc aagaagaaat tcagcgtgat atcggtgtcg ctaaacgcga attcccacta
     1321 tttgtacctg atgaaagttt agtcgctgaa cttaaagaaa aatatacagg taaactcact
     1381 gaagctatca gtgtctttga caaattagaa cgcagtgcag caattgaagc tgttaaaggc
     1441 gaaatggttg aagaatatga agctaaggaa tatgaaagtg atgacgacaa acgccgtgtt
     1501 ataagtgatg ttaaagcgtt acacgaagta atggtgagcg atgaaattcg ccggcttatt
     1561 actcacgaaa aagttagacc tgatggtcgt aaagtcgatg aaattcgtcc gcttgatagt
     1621 caagttgact tattaccaag aacgcatggt agtgcacttt ttacccgcgg acaaacacaa
     1681 gtcttatcag ttgtgacgct tgggccaaag agtgacgaac aaattattga taacttaact
     1741 gatgaagaat cacggcgctt tatgcaccat tataacttcc cgccttatag cgttggcgaa
     1801 attaaacgat taggtggtcc agcacggcgt gaaattggac atggtgcttt aggtgaaaga
     1861 gctttacttg ccgttattcc aagtgacgaa gaattcccat acaccatccg tgttgtaagt
     1921 gaagtacttg aaagtaatgg ttcaagttca caagctagta tttgtgcctc aagtatggca
     1981 ctgatggccg ctggtgtccc aattaaagat atggttaccg ggattgccat gggcttaatt
     2041 acaagtggac cacttgatgg taatcatccc tataccattc ttactgatat ccaaggctta
     2101 gaagatcact tcggtgatat ggactttaaa gttgctggta ctagtaaagg aattactgca
     2161 ttacaaatgg atattaaagt acgcggaatc agcaaagaag tcttagccga aagcttagcg
     2221 caagcgaaaa aagctcgtga agaaattcgc gcaaatcaat taagtgcgat tagtgcacca
     2281 cgtgaagatg tcgctgaatt tgcaccaaag atgggtaaga tgacaattga tgttgataaa
     2341 atccgtgaag ttatcggtac tggcggtaaa gtcattaata agattatcga agataatgat
     2401 ggcgcgaaga ttgatattga tgacgacgga ttagtcgttg tctatcacat gaaccgtgcg
     2461 acgattaata atgtgcttgc cacgattgaa gcaatcgtta aagaagcaaa ggctggggaa
     2521 atttacgaag gtaaagtaac ccgcgttgaa ccttatggtg tctttgttaa tctctttggt
     2581 aatgttgacg cactagtgca tgttagtgat ttagactggg cttatgttga tgatgcatca
     2641 aagttatata aaattggtga tacattaaaa gttaaagtta ctggtgttga tgaaaaaggt
     2701 aaagttaaag cgagtgtaaa agtctttaaa gctaaaccag aaggatacac cgaacgtaaa
     2761 agtggcggtc gccgtaataa taaatagtaa ttactattga aaattcttta agcctcttaa
     2821 ccatatggtt tggaggtttt ttaatagatg taaataattt aaggctttaa ttcctaatta
     2881 tttttataat tagtgtatga taaatatata tagaaaagca cggagttgct ctaaggtgaa
     2941 tttttatgta tgacattatt attattggcg ctggggtaat cggcgcttta attggacgcg
     3001 aagcaagtaa atatgaactt aatattttaa tgctcgaaaa agataacgat gtaggtaatg
     3061 gcactagtgc tgctaatagc gcaattgtgc atagtggtta tgacccaaag ccagggacat
     3121 taaaagcaaa atttaatgtt gcttcggtgc cgctttacaa agatttatgt agtgacttag
     3181 atgtggaatt taagcggatt gggagtttag ttgtggcaca aggtgaagaa gaattgcccc
     3241 gacttgatga attaaaagca cgcgctgagc ttaacggcgt ggaagtcaaa atgttaaata
     3301 aagcagaagt acatgctctt gaaccaaatt tacatgatca tattgtcggc ggcttatttg
     3361 caccgacagc agggatagta aacccttttg aactaacggc acacgcgatt gaaaatgcaa
     3421 ttgaaaacgg gatgacttta aagcttaatg aagaggtagt agcgataaaa aagcttgaag
     3481 acggctttaa agttacgacg aagaatggtg attattttag taaagttgtg attaatgcag
     3541 cgggtgtatt tgccgatgat atcattaata tggtaagtaa ggctgatttt gaaattactc
     3601 cgcgaaaggg tagttatttt gtccttgata aattaacggt tccacttgta aatacagtat
     3661 tattcccgct gcctaatata catagtaaag ggattttagt tgtcccaaca accgctggta
     3721 atacactgat tggaccgaca agtg
//
LOCUS       DAOPCD010000085         3741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_19926_length_3741_cov_3.566468, whole genome shotgun sequence.
ACCESSION   DAOPCD010000085 DAOPCD010000000
VERSION     DAOPCD010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19926_length_3741_cov_3.566468"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PLQ55_02840"
     CDS             <1..1077
                     /locus_tag="PLQ55_02840"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPK28899.1"
                     /translation="VDESVANEFDVRKLTFFSEDESVATINKYGVIKGISVGETYVGV
                     VGDDRKFKVSVKSNDTPLISPTSITLEFDEIAYVYDKIPLNYTFDVENVTDPSLTFVS
                     SNDIVATVIKEDDNYFIKTPKIKGTATITAYLNSNFAIRATKDIIVNNVMPTSVNFTN
                     VPTELITGQRVNVGYDLNHSLAKESLSVTDKRVIFTSSDNSIARVSTGNMFGTIVALK
                     EGVVTISATSVADPTISASFSIKIIPRPFINDDNFSDFQGFVRKAVGHFFLFFIDGIF
                     GFFTFYLFLKDKKSTRLITVLSLITGVFIAALSEFIQLFVPGRAGTITDVIIDSAGYL
                     CATMLMLLIVYLINKNKSNKNVQQ"
     gene            complement(1164..1637)
                     /locus_tag="PLQ55_02845"
     CDS             complement(1164..1637)
                     /locus_tag="PLQ55_02845"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HPK28900.1"
                     /translation="MIGINHHEIENEVTATLHAISVDNDFEFQLPLDVNAKTLGVTLG
                     VPVVISLYNEAFYYQITISDYRRSNFMQVSMRLFIGEIPNPQKLYALINKLNTKLLYA
                     KVVLDEEEDNTLILEHDFTAASVEQLSESIYDFLVDVVDEDVSPLIEELLKEIRD"
     gene            complement(1655..2392)
                     /locus_tag="PLQ55_02850"
     CDS             complement(1655..2392)
                     /locus_tag="PLQ55_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28901.1"
                     /translation="MILLLGTTPDDILYIKNRMIMKEKGAVNKDEHFYFVGEYGGKEI
                     CMTYTGHSNIMSAVLTSYMIRKFNPYLVISIGSAQSASSNLNQGDLFIAERVYIADLD
                     YNNYTNLPLRDAIHMHPYYVSEEVYIKHFELLNSATGNYKLVRGPVISVNNFVKEKAE
                     AERIIARQENYIEGPTVFDTEMGGIVACCRFYGIPWLYLRSINYEIGKEEQLISFVRK
                     GVEAQPRIGELVEQLFIFLNTSLEESI"
     gene            complement(2392..>3741)
                     /locus_tag="PLQ55_02855"
     CDS             complement(2392..>3741)
                     /locus_tag="PLQ55_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPK28902.1"
                     /translation="QIRFNYATFELVEGINSERAFLAYVAKDIKRNRGKANALVAFAA
                     TRFKKIVFTRYGYEKGGEVLGLVYETFNTLKTKYPHSYYGYDYNENFLIYLRDTPYEQ
                     VQEYVNDLIEEINKTLFENRNEVKFVADFGVSYCQNENDEFITAASMLQRALIAADHG
                     RAESDLGGLTLYEPKMYERNKRNVQLGLQIERSLELEQFELHYQPKYDLKLGRFAGAE
                     ALVRWRHPELGLLSPANFILFAEQSDLIIKIDHYVLEKVCKQLDEWRGKGQRLLPISV
                     NISKRTLFTGNIINHLIKVTDKYNINPMLLEIEIVESPSLQDILLLLSIVKKFKNLKV
                     KIAIDDFGTGYSSLSYIKKIPFDVIKIDKAFLDDVDIDQKSRLMIKNIIDLAHILDAY
                     VVMEGVQDERQVKMLEEMGADSIQGFYYSRPLPPEEYQAFLEDNLFEANKKKKRGRK"
BASE COUNT         1149 a          678 c          623 g         1291 t
ORIGIN      
        1 gttgatgaaa gtgtcgctaa tgaatttgat gttcgtaaac tcactttctt tagtgaagac
       61 gaaagtgttg caactattaa taagtacggt gttattaaag ggattagcgt tggcgaaact
      121 tatgttggtg ttgttggtga cgaccgaaaa tttaaggtga gtgtgaagag taatgataca
      181 ccgctaatat cgccaacttc aattacttta gaatttgatg aaatcgctta tgtttacgac
      241 aaaatccccc taaattatac atttgacgtg gaaaatgtta ctgatccatc actaactttt
      301 gtatcaagta atgacattgt tgctactgtt attaaagaag atgataatta ctttattaag
      361 acacctaaaa taaaaggtac cgctacaatt actgcttact taaatagtaa ctttgcaatc
      421 agggccacaa aagatattat cgttaataat gtgatgccaa cttctgttaa ttttactaat
      481 gttccaactg aattaattac tggtcagcgg gtaaatgtcg gttatgactt aaatcattcc
      541 ttagcgaaag aatcgttaag tgtgactgat aagcgtgtta tttttacttc aagtgataac
      601 agtattgcgc gcgtatcaac tgggaacatg tttggcacta ttgttgcttt aaaagaaggc
      661 gttgttacta ttagcgcgac aagtgttgcc gaccctacta tcagcgcatc ttttagtatt
      721 aaaattattc cgcgaccctt tattaatgat gataatttta gtgacttcca aggctttgtt
      781 cgaaaagcag ttggtcactt cttcttattt tttatcgacg ggatctttgg atttttcact
      841 ttttacttat ttttaaaaga taaaaaaagc actaggttaa taacagtgct ttctcttatt
      901 actggtgttt ttattgctgc tcttagtgaa tttattcagc tttttgttcc aggacgagca
      961 ggtactatta ctgatgttat tatcgatagt gcagggtact tatgtgcaac aatgttaatg
     1021 ctgttaatcg tgtatctaat aaataaaaat aaaagcaata aaaatgtcca acaataataa
     1081 aaataagtgt taaatcacgt ttttgaactt tttaaatggc aaaatatcac gtttttgaac
     1141 ttttcgaata tttttatagt gttttaatca cgaatttctt ttaataattc ttcgattaat
     1201 ggtgaaacat cttcatcgac aacatcaacg agaaagtcat aaatcgattc acttaattgt
     1261 tccacacttg cagcggtaaa gtcgtgttct aaaattaatg tgttatcttc ctcttcatct
     1321 aaaacaactt tggcatagag taatttagtg tttaatttat taataagcgc atataacttt
     1381 tgtggattag gaatttcgcc aatgaaaagt cgcatactca cttgcatgaa attagagcgg
     1441 cgataatcac taatcgtaat ttgataatag aaagcctcgt tataaagact aatcacgact
     1501 ggtacaccta aagtaacacc aagtgtttta gcgttaacgt caagtggtaa ttgaaattca
     1561 aaatcattat caactgaaat cgcgtgcaaa gtcgctgtta cttcattttc aatttcatgg
     1621 tgattaatgc caatcatgac ttactccttt ctttttaaat actttcttca agcgaagtat
     1681 ttaagaatat aaatagttgt tcaactagct caccgatgcg tggttgtgct tcaacacctt
     1741 tacgcacaaa ggaaatgagt tgttcttctt taccaatttc gtaattaatt gagcgaagat
     1801 aaagccacgg tatcccgtag aaccgacaac acgctacaat cccgcccatt tccgtatcaa
     1861 acactgttgg cccttcaata tagttttctt gacgggcaat aatgcgttca gcttccgcct
     1921 tttctttcac aaagttatta acactgatga cgggacccct tactaattta tagttccctg
     1981 ttgcactgtt taatagttca aagtgtttaa tatacacttc ttcactaaca taatatgggt
     2041 gcatatgaat tgcgtctctg agcggtaaat ttgtgtaatt gttataatct aagtccgcga
     2101 tataaacgcg ttcagcaata aaaagatcgc cttgattaag attactactt gcactttgtg
     2161 cactgccaat tgaaataaca agataaggat taaatttacg aatcatgtaa cttgtgagca
     2221 ctgccgacat aatattacta tgcccagtgt aagtcataca tatttcttta ccaccatatt
     2281 ccccgacaaa gtaaaagtgt tcgtctttat taaccgcacc tttttctttc ataatcatcc
     2341 ggtttttaat atagagaatg tcatccggag ttgttcccaa tagtaaaatc attatttccg
     2401 tcctcgcttt ttcttcttat tagcttcgaa gaggttatct tcgaggaaag cttgatattc
     2461 ttcaggtggt agtggtctac tatagtagaa cccttggatg gagtcagccc ccatctcctc
     2521 aagcatcttc acttgtcttt catcttgtac accttccata acgacatagg cgtcgagaat
     2581 gtgcgctaaa tcaataatgt ttttaatcat taatcgtgat ttttgatcaa tatcaacgtc
     2641 atcaaggaaa gccttatcaa ttttaataac atcaaaggga atctttttaa tataactaag
     2701 cgaagagtaa ccagtcccaa agtcgtcaat ggcaatttta actttcaagt ttttaaattt
     2761 cttcacaatt gagagtaaaa gtaatatgtc ttgcagtgat ggtgattcaa cgatttcaat
     2821 ttcaagcaac atcgggttaa tattatattt atctgttact ttaattaggt gattaataat
     2881 attcccagta aagagtgttc gttttgaaat gttgacactg attggtaata agcgttgtcc
     2941 tttaccccgc cattcatcaa gttgtttaca cactttttcc agcacataat ggtcaatttt
     3001 aataattaag tcactttgtt cggcaaataa gataaagttt gcaggtgaga gtagtcctaa
     3061 ctctggatgg cgccatctaa caagtgcttc agcgcccgca aagcggccta attttaagtc
     3121 atattttggt tgatagtgca gttcgaattg ttctaattcc aaactgcgtt caatttgtaa
     3181 accaagttgg acatttcgtt tattacgttc atacatcttt ggttcataaa gtgttaaacc
     3241 acctaaatca ctttcggcac gaccgtgatc agctgcaatg agtgcccgtt ggagcatcga
     3301 cgccgcggta ataaattcgt cattttcatt ttgacagtaa ctcacaccaa agtccgccac
     3361 aaattttacc tcattacgat tttcaaataa tgttttatta atttcttcaa ttaagtcatt
     3421 cacatattct tgtacttgtt cataaggtgt atctcttaaa taaataagga agttttcatt
     3481 atagtcgtaa ccgtaataag aatgcgggta ttttgtttta agtgtattaa atgtttcata
     3541 cactaaacct aaaacttcgc cgcctttctc atacccatat cgcgtaaaga caatcttttt
     3601 aaaacgtgtt gccgcaaaag cgacaagtgc atttgcttta cctctattac gcttaatatc
     3661 tttagcgaca taggctaaaa acgctcgttc tgaattaatc ccttccacta attcaaatgt
     3721 cgcataatta aagcgaattt g
//
LOCUS       DAOPCD010000086         3690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_20273_length_3690_cov_4.688858, whole genome shotgun sequence.
ACCESSION   DAOPCD010000086 DAOPCD010000000
VERSION     DAOPCD010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20273_length_3690_cov_4.688858"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLQ55_02860"
     CDS             complement(<1..345)
                     /locus_tag="PLQ55_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28903.1"
                     /translation="MNKYEDKLKKIKKDIPEYPKTLDPKVLEAAHKAPRSKRPGFPKL
                     LIPSLLTVTAIALSTFFVTKYVYYWNNPVNSSSDTSDPTDKSSEPTNRDFAVMKSLSI
                     DGTSLDPDVIGEG"
     gene            complement(338..802)
                     /locus_tag="PLQ55_02865"
     CDS             complement(338..802)
                     /locus_tag="PLQ55_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPK28904.1"
                     /translation="MTNEQFAIVYETYKVMVFQIAYSYLQSVEDAEDAVLESFMRLLR
                     INKNFVSSDDERRYLTRIVVNVSKDMLRKKRPVSISDPIVAISDEPLNENEYIWDEVN
                     KLKTKYKDVIILRYVNEYSFSEIAKVLKISASAARKRHERALKMLKEELEHE"
     gene            complement(1233..2786)
                     /locus_tag="PLQ55_02870"
     CDS             complement(1233..2786)
                     /locus_tag="PLQ55_02870"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HPK28905.1"
                     /translation="MNLEDALKRIAILEKENEALQLENEEQRLLIFKQQQKLNELLKD
                     KEIVREKLIIERTKPFVAKSEKINEIVINEPEHILQENKHTKARVGRKKGGKNFANVD
                     LEAAVIDTVYEDPDSLVCPACSNDLTLASQKERYIIEVIPSTIKVTKVIKRSYKCKHD
                     QTFVYPLSQEVFPGSVLTPSFAAYIAYHKYELGIPFHHLEKHLTNVLNVPISKQLMAT
                     WMQTLATKLTPVYNAMKRDLLSNSVKVIHADETTLSIAKRPENALNRKKSYVYVYASS
                     YFDQQIHLYDFHESRSIDLTAKWLSKYDGYIVCDDYAGYTKLAKNNQNIKLQRCWAHA
                     RRRFSDILKALPTTEVKTTLSYKILTLINELFAYEAKYKTDAVLPSEICAKRHHDQIP
                     LLNELKTYVFETPLSPNSALEGAVHYVRKIWDELLTYLDYPYLEISNNLAERAVKPFV
                     INRKVFMTAGSYAGAKYTTIIFSIIRTALINHLNIEKYLIYLLENLDKQPLDELLPYA
                     PKLPNKLKV"
     gene            complement(2857..3192)
                     /gene="tnpB"
                     /locus_tag="PLQ55_02875"
     CDS             complement(2857..3192)
                     /gene="tnpB"
                     /locus_tag="PLQ55_02875"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HPK28906.1"
                     /translation="MIDLNKVRNIFLYTNKIDMRMGIIKMESILSLSFSPLEIHRSVF
                     IFVSKSRKMVKIYYEDEYGKWLFINKLSYARFNVPALEESFTITKDDLAYLLKGVALI
                     SERRKQVSL"
     gene            complement(3189..3557)
                     /locus_tag="PLQ55_02880"
     CDS             complement(3189..3557)
                     /locus_tag="PLQ55_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPK28907.1"
                     /translation="MGKYKTDYERLETVKEFKMSKLSVSEFARQKGINRTTLRDWINA
                     YDNIDGSFVRLNKVLDSSRKSVLLSNDDVNVSLLKPEEIRAKSKNFTRFDHSVVVIEV
                     AQVKVTTSLQQALAILEKIL"
BASE COUNT         1111 a          644 c          690 g         1245 t
ORIGIN      
        1 tccctcacca atgacatcag gatctaatga ggttccgtct attgataaac ttttcattac
       61 ggcaaaatca cgatttgttg gttcacttga tttatcagtc ggatcagaag tgtcgctaga
      121 agaatttaca ggattattcc aatagtaaac atattttgta acaaaaaagg tggataaagc
      181 aattgctgtc actgttaata atgaggggat aagcagtttt ggaaatcccg gtcgcttgct
      241 gcgtggtgct ttgtgagcag cttctaaaac tttcggatct aaggtttttg gatactcggg
      301 aatatccttt tttatttttt tgagtttatc ttcatattta ttcatgttca agttcctcct
      361 ttaacatttt gagcgcacgt tcgtgccgct ttcttgctgc acttgcgctt atttttaata
      421 ctttagcgat ttcactaaaa ctatattcat tcacatagcg caagataatc acatctttat
      481 atttagtttt aagtttgtta acttcatccc aaatatattc gttttcatta agtggctcgt
      541 cactaatcgc aacaatcgga tcgctaattg acactggcct tttcttgcgg agcatatctt
      601 tacttacatt aacaacaatt cttgttaagt aacgtcgttc atcatcgctt gatacaaagt
      661 ttttatttat tcgtaagagc cgcataaacg attccagcac agcatcttct gcatcttcga
      721 cactttgtaa gtaagagtag gctatttgga aaaccataac tttgtaagtt tcataaacga
      781 ttgcaaactg ttcgttagtc atattctcct tacacaatac aaacgtaact taaaatcatt
      841 ttgtgacatt aagtgtaatt tgaccctttc tattagtaat aataattagt tctctataat
      901 acataacgat tataaagcgt cttttgtgac aaaaaacttt tttttggtaa aaacattctt
      961 aaatcaagtt tatacttttt taaaatagat tttgttttaa tgagttttag cggcaaactc
     1021 aacaactcat aaaacatagt aaagttcgga tttttagaac atttgtgatt ttcgtattta
     1081 tcctaattaa ctataaaaac accccttcca cagttaaata ttattgacac ttatgtatag
     1141 gtaaaaaatt aacacttctt taaaaatgag aaaaaaaggc acgaaaagtg cttttttgat
     1201 agtggggcga agtctgacag tggcggactt tgtcaaactt ttagcttatt tggtaattta
     1261 ggagcgtacg gcaagagttc atctaacggt tgtttgtcga gattttctaa aagataaata
     1321 aggtattttt ctatattcaa atggttaatt aatgcagtgc gaataatgga aaagataatt
     1381 gttgtgtact tagcgcctgc gtatgatcca gcggtcataa agactttgcg attaatgaca
     1441 aatggtttta cggcgcgttc agcaaggtta ttcgaaattt caagatacgg ataatcaaga
     1501 tacgtcaaaa gttcatccca aattttccga acataatgga cagcaccttc aagggcagag
     1561 ttcggcgaga gtggagtctc aaaaacatag gttttaagtt cattaagcag cggaatttgg
     1621 tcatgatggc gttttgcaca tatttcggaa ggaagaacag cgtccgtttt atatttagct
     1681 tcgtaagcga aaagttcgtt aatgagcgtc agtattttgt aacttaatgt tgttttaact
     1741 tcggtcgtgg ggagtgcttt taagatgtca ctgaagcgac gacgcgcgtg agcccagcag
     1801 cgttgtaatt taatattttg gttatttttt gcaagtttgg tgtaaccagc ataatcatca
     1861 cagacgatat agccgtcata cttagaaagc cacttagcgg ttaaatcaat actgcgtgat
     1921 tcatgaaaat cataaagatg aatttgttgg tcaaaataac ttgaagcata gacataaaca
     1981 tagcttttct tgcgatttaa ggcgttttcc gggcgtttag cgatacttaa tgtcgtttca
     2041 tccgcgtgaa tgactttaac cgagttactg agtaaatcgc gtttcatggc gttatagacc
     2101 ggtgttaact tcgtcgcgag tgtttgcatc caggtggcca taagttgctt cgagatgggc
     2161 acgttaagca cattggtaag atgtttttcc aaatgatgaa aaggaatccc aagttcgtat
     2221 ttgtggtaag caatataggc agcaaaagag ggggttaaca cggagccagg gaaaacttct
     2281 tgactaagcg gataaacgaa tgtttgatcg tgtttacatt tgtaggaacg tttaattact
     2341 ttcgtcactt taatcgtgga aggaattact tcaatgatgt aacgttcttt ttggctagca
     2401 agcgttaagt catttgaaca ggcgggacaa acaagcgaat ccgggtcttc gtaaactgta
     2461 tcaatgacag cggcttctaa atcaacatta gcgaaattct tcccaccttt tttgcgacca
     2521 acccgcgctt ttgtgtgttt gttttcttgt aaaatatgtt ctggttcatt aatgacgatt
     2581 tcgttaatct tttcactttt agcgacgaac ggtttcgtgc gttcaatgat aagtttttcg
     2641 cgaacaattt ctttgtcttt gagtagttca ttaagttttt gttgttgctt aaaaatgagg
     2701 agacgttgtt cttcgttttc aagttggagg gcttcatttt ctttttcaag aatagcgatg
     2761 cgtttaagtg cgtcttctaa attcataact atattttagc acatataaaa aaagttagtt
     2821 atatataata taactaaaat ttttataaag cgtgatttaa agcgaaactt gcttgcggcg
     2881 ttcgcttatg agcgcgacac ctttaagtaa ataagcaaga tcgtctttcg tgatggtgaa
     2941 cgactcttca agcgcgggga cattaaaacg agcgtaagaa agtttattaa taaagagcca
     3001 ttttccgtat tcatcttcgt aataaatttt aaccattttg cgacttttac taacaaaaat
     3061 aaacacggag cggtgaattt cgagcggaga aaaagaaagc gaaagaatgg actccatttt
     3121 aataatcccc atccgcatat cgattttatt cgtgtagaga aaaatattgc gaactttatt
     3181 aagatcaatc ataatatttt ctccaaaata gctagtgctt gttgtagcga agttgtcact
     3241 ttcacctgcg cgacttcaat gacaacaaca gaatgatcaa accgcgtgaa gttcttcgac
     3301 ttagcacgta tttcttccgg cttaagcaga ctaacattaa catcgtcatt actaagcaag
     3361 acactttttc ttgaactatc aagtaccttg tttaaacgaa cgaaagaacc atcaatatta
     3421 tcgtaggcat tgatccaatc gcgtaatgtc gtgcgattaa tgcccttttg gcgagcgaac
     3481 tctgagaccg acagcttact catcttaaat tctttgactg tctctagtcg ttcataatca
     3541 gttttatatt tacccataaa aaactcctac ctttctaagt aagagtttat gtaattcatg
     3601 ttagtttgaa aatggtgtta tttttttacc tatacacact tattgttcat ctacgccaaa
     3661 tctacgccaa atctacgcca ggcaaagaaa
//
LOCUS       DAOPCD010000087         3667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_20455_length_3667_cov_3.039867, whole genome shotgun sequence.
ACCESSION   DAOPCD010000087 DAOPCD010000000
VERSION     DAOPCD010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20455_length_3667_cov_3.039867"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1924)
                     /locus_tag="PLQ55_02885"
     CDS             complement(<1..1924)
                     /locus_tag="PLQ55_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPK28908.1"
                     /translation="MRILELNIDSFGKLENYNLKFDQKLTIINEDNSFGKTTIAQFIK
                     AMFFGMKTAKGKGKRLSLPRFKYLPWGKTTYGGNLTFVYNGRTYTVTRTFGESLSNDT
                     IAVKDLARNVNIDELNGIKITRDNLGDIIFGVNSESFEKLNFIQQLEVLYSSDEKIHD
                     DINVKLRGLFGHTTEDSDYTSAFNILDDAIKELSPGNQRRNSLYNKTDRELNATNNLV
                     FEAENAVNLISENSDELVALKRQKAENDKLKTKVENDLKLVANKKILEKDLKDYNALN
                     DEINNLNLQIEENKKIFAGNKVEDTNLVYLEELSTDLSAKESDFNKFKDEAQTEINSI
                     NAALTTLESTNVEVLFIKRAHKETSEEITKLENEVTLLNANIKRFRFLDIFLSIITLS
                     IYFFVLKSKNNKLRKEIALLNKTIDELNVKLENYAAELATMTSTESDIKLKQKLDEWT
                     QKVNQKQKELEAFKEHVFDVFRKYSVKVNSVSDVQVAYFLINSHYEKHLALNKKLNDV
                     NFKLKNYDIKKINSDLNSFALLDEEELNRNKSNVDIKNDELIKKITALEKQIEANEDL
                     ASNLENYKFERDELRTTMQNYEQKKELLTIARTLLEHANQQLAARYLTPLQNNVNDLL
                     TKIKLEGYSVMFDGNSE"
     gene            complement(1921..2982)
                     /locus_tag="PLQ55_02890"
     CDS             complement(1921..2982)
                     /locus_tag="PLQ55_02890"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HPK28909.1"
                     /translation="MKIIHTADIHIDSKMNRYGGDKTERRIREIKNAFFRMIDYGVKH
                     NVKVILIAGDLFDNKEVKLATLREVLTKITSAPTIDFIYLAGNHDDPAVFNHEKLAEL
                     PSNLKIVTKENNTFLYDDVCIKAYDNYADEDYTKTLIFDPNKYNILMLHGTEAEIPLN
                     NLKGKNIDYLALGDIHIPDIAAKRLDARGIYAYSGVLEPRGFDEVGERGFFVLEIQKN
                     KLIRREFIKNNSRTYHIIDVDISALNNTLEIELAIKKATNKIPSDDIVRLKLTGKYDY
                     ELIKDINALREYLESKFYYAELIDDSKVNLNTIDFANEVSLRGEFFRLIEASNYSPEV
                     KEELLKLGLNALKGRKIDL"
     gene            complement(3144..3551)
                     /locus_tag="PLQ55_02895"
     CDS             complement(3144..3551)
                     /locus_tag="PLQ55_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPK28910.1"
                     /translation="MESKYRKNNKNITKDLKLNQDELAGIHNEKKHIGKELAIGVLNS
                     ITIGLGGFLLSFLILSFINMGTHSPLLLAITIGTSLMGGMFISAAAGVFIPIILEKMK
                     VDPAIASGPIISTLNDLFALLIYFGIATLIFLL"
BASE COUNT         1124 a          615 c          525 g         1403 t
ORIGIN      
        1 tttcagagtt tccgtcaaac attacagagt agccctctaa tttaattttc gtaagtaagt
       61 cgttaacatt attttgaagc ggggttaaat accttgctgc tagttgctga ttagcgtgtt
      121 ctaataatgt tctagcgata gttaataatt cttttttctg ctcatagttt tgcattgtag
      181 tgcgtaattc gtcgcgttca aacttgtaat tttcaaggtt actcgctaag tcttcatttg
      241 cttcaatttg tttttctaaa gctgttattt ttttgattaa ttcatcgttt ttgatgtcga
      301 cattactctt attcctgttt aattcttctt catctagtag ggcgaaagaa tttaaatcac
      361 tatttatttt tttaatatca taattcttaa gtttaaaatt tacatcatta agctttttat
      421 taagcgctaa atgtttttca tagtgactat taataaggaa ataagcaact tgaacatcac
      481 taacactatt aactttaacg gaatacttac gaaatacatc aaaaacatgt tctttaaaag
      541 cttctaattc tttttgtttc tggttaactt tttgtgtcca ctcatcaagt ttttgtttaa
      601 gtttaatgtc actttctgtg ctagtcattg tcgctagttc agcggcataa ttttcaagtt
      661 ttacattaag ttcatcaatc gttttattta aaagcgctat ttcttttctt aacttattat
      721 tcttactttt aaggacaaag aaataaattg atagcgtgat aatgcttagg aaaatatcaa
      781 ggaaacgaaa tctttttata tttgcattaa gtaaagttac ttcattttct aatttagtaa
      841 tttcttcact agtttcttta tgcgctcttt taataaatag gacttcaaca tttgtacttt
      901 caagagtagt tagagcagcg tttattgaat taatttctgt ttgtgcttca tctttaaatt
      961 tattaaagtc actctctttt gccgacaaat ccgtgcttaa ttcttctaaa tacactaaat
     1021 ttgtgtcttc aactttatta cccgcaaata tttttttatt ttcttcaatt tgtaaattaa
     1081 ggttatttat ttcatcattt agtgcattat aatctttaag gtctttttca agtatctttt
     1141 tattcgcaac gagttttaaa tcattttcaa ctttagtttt tagcttatca ttttctgctt
     1201 tttgtctttt tagcgcaact aattcatcag agttttcact aattaaatta acagcgtttt
     1261 ctgcttcaaa cacaagatta tttgtggcat tgagttcacg gtcagttttg ttatatagtg
     1321 aatttcttcg ctggttacca ggtgaaagtt cttttatagc atcatcaaga atattaaatg
     1381 cggaagtgta atcactatct tctgttgtat gcccaaacag accgcgtaat tttacattaa
     1441 tatcatcatg aattttttca tcactactat ataaaacttc taattgttga ataaagttta
     1501 atttctcaaa actttcacta tttacaccaa agatgatatc accgaggtta tcacgcgtta
     1561 ttttaatacc atttaattca tcaatattta cattgcgcgc taagtcttta actgcaatcg
     1621 tatcattaga gagcgattca ccaaaggtgc gggtaacagt ataagttcgc ccgttgtaga
     1681 caaaagttaa gttaccacca taggtggttt taccccacgg taaatattta aagcgtggta
     1741 gtgatagtct ttttcctttt cctttagcgg ttttcatccc aaagaacatc gctttaatga
     1801 attgcgcaat cgttgtttta ccaaaactat tatcttcatt aatgattgta agcttttgat
     1861 caaatttaag attgtaattt tcaagcttac caaaactatc aatattaagt tctaatattc
     1921 tcataggtca attttccttc cctttagtgc atttagaccc aattttaaga gttcttcctt
     1981 aacttcaggg gagtaatttg aagcttcgat taggcgaaag aattcaccgc gtaatgatac
     2041 ttcgtttgca aaatcaatag tgttaaggtt aaccttagaa tcatcgatta actctgcata
     2101 ataaaattta ctttctaaat attcgcgcag tgcatttata tctttgatta attcataatc
     2161 atatttacca gttaatttta gacgtacgat atcatcacta ggaatcttat tagtggcctt
     2221 cttaattgct aactcaattt caagagtgtt gtttaatgca ctgatatcga catcaataat
     2281 atgataagta cgactattat ttttaataaa ttcgcgtcta atcagcttat ttttttgtat
     2341 ttctaaaaca aaaaagccgc gttcacccac ttcatcaaac ccgcgcggtt caagcacacc
     2401 tgaataagca tatatgccgc gcgcatctaa tcttttcgcg gcaatatcgg ggatatggat
     2461 gtcgccaagc gctaagtagt caatattttt gccttttaaa ttatttaaag ggatttcagc
     2521 ttcagtgcca tgtagcatca agatgttata tttatttgga tcaaagatta aggttttagt
     2581 gtaatcttca tctgcatagt tatcataagc tttaatacat acatcatcgt aaaggaatgt
     2641 attattttct tttgtcacaa ttttaagatt tgaaggtaat tccgctaatt tttcatgatt
     2701 aaatacagct gggtcatcat gattacccgc taaatatata aaatcaattg ttggtgcact
     2761 tgtaattttt gttaatactt cacgtaaggt agcaagtttt acttctttat tgtcaaataa
     2821 gtctcctgcg attaagataa ctttaacatt gtgcttaact ccgtaatcga tcatgcgaaa
     2881 aaaagcattt ttaatttctc taatgcgtct ttctgtttta tcgccaccat accgattcat
     2941 ctttgaatca atatgaatat ccgctgtatg gattattttc atacttaccc ccgttaactt
     3001 acatttagtt aacttatttt atcataatac ctagccatta aagtgaatat agcattaata
     3061 aatgctatta gccttcatta ataaaaaaag acatcatgtg gcgatgtctt ttatattgtt
     3121 tgtaagcatc tcttctcttg ctcttatagt aagaagatta atgttgcaat tccaaaataa
     3181 attagtaatg cgaataagtc atttagcgtc gagataattg gaccactagc aatcgcagga
     3241 tcaaccttca tcttctctaa aataattggg ataaaaacac cagcggcagc agagataaac
     3301 atcccgccca taagcgaagt cccaatcgtg attgctagaa gtaacgggct atgcgttccc
     3361 atattaatga aactaagaat aagaaatgag agtaaaaaac caccaagacc aatagtgatg
     3421 ctatttaaga ctccgattgc tagttctttg ccaatgtgtt ttttctcatt atggatacct
     3481 gcaagttcat cttgatttaa ttttaaatct tttgttatat ttttattatt tttacgatac
     3541 ttgctttcca acaggttttt gcacaaaaga atataaataa aagtaaaggg ttgtcaaaag
     3601 tcagtacgtt aaataataaa gataaagata gtagtaatac cgaattaaaa acatcaccat
     3661 ctggtat
//
LOCUS       DAOPCD010000088         3591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_20959_length_3591_cov_3.795532, whole genome shotgun sequence.
ACCESSION   DAOPCD010000088 DAOPCD010000000
VERSION     DAOPCD010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20959_length_3591_cov_3.795532"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLQ55_02900"
     CDS             <1..694
                     /locus_tag="PLQ55_02900"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004802385.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPK28911.1"
                     /translation="LRERIESLSEFNPMMGHRGVRLAVSYPEIAVMQTTAIISAALRA
                     QKDTGAAIHPEIMIPLVLDVKEYKYVEEYVRKTADALIKKSGQKLHYLVGTMIEIPRA
                     TLLADQIAKEAEFFSFGTNDLTQMTFGFSRDDATKFIPDYTEARIFEQDPFQTLDTEG
                     VGQLVRLGVERGRKGNPDVHLGICGEHGGDPNSVEFFDSVGLDYVSCSPYRVPIARLA
                     AAQAAIKHKKAK"
     gene            complement(799..2187)
                     /gene="glmU"
                     /locus_tag="PLQ55_02905"
     CDS             complement(799..2187)
                     /gene="glmU"
                     /locus_tag="PLQ55_02905"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HPK28912.1"
                     /translation="MKRNAIILAAGKGTRMKSLNQEISKVGFEILGKPLINWVLDAAK
                     PHVTGRTVVIVGFGGEHTSKLVEGKAEIAWQHEQKGTGHAIIQTAPLLEKEKGDTLIL
                     SGDVPLITEHSISGLLNLHTKEKNDLTILTAKPDNPFGYGRVLRAPNGEVTRIVEQKD
                     LTGNEGDISEVNAGMYVVNNQKLFQELKNLKPNNKQGELYLTDIIGLFKERGYKVGAF
                     VLSDPDEMLGTNDRAQLAEATELLKKRINKAHMLNGVTLEDPNNTYIGPDVTIGQDTI
                     ISPNTTILGNSTIGVANEIGPNTYLKNMKIGDYNSIIYSYLVDSEICNNVTIGPFARM
                     RQKALVKDGSHIGNFVELKNTTFHEGVKAGHLAYMGDAELGAHTNVGAGAITANYDGV
                     NKSKTKVGENVFIGTNTTLVAPLQIGEGSYLAAASIINKDVPKDTLAIARSRQENKEG
                     YAKIIREKQKKK"
     gene            complement(2197..3048)
                     /locus_tag="PLQ55_02910"
     CDS             complement(2197..3048)
                     /locus_tag="PLQ55_02910"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28913.1"
                     /translation="MIVTANARLSISVETTLNPLTNALEIKSLVLPIEFSDTITIDVF
                     NTAFMDIVHYTNKALHVKEKRIIDEALLKMREQFGYKQNFSVKIHKNIPTGHGLGSGA
                     SDATAVVKVVAHLAKLKPTPEQYYNLAVKIGKDVPYFLVNKPAVYNLNKQEVTPIKFK
                     HNPQVLLIIHPDIYEKENVVKDFLDYGESLQKPLEKVVELAESGSLRELGSVLFNDFS
                     ETIIKHTPALRTVLNDLKQLNVEAYGIAGTGTTIFALSDNRHLLRFISDKYRKQNYQT
                     VLTKVIR"
BASE COUNT         1002 a          691 c          628 g         1270 t
ORIGIN      
        1 tcttcgcgaa cggattgaat cgctttcaga atttaaccca atgatgggtc accgtggtgt
       61 gcgtttagct gtttcttacc cagaaatcgc tgtgatgcaa acaactgcta ttattagtgc
      121 cgccttacgt gcacaaaaag acactggtgc cgctatccac ccagaaatca tgattccact
      181 agtgctagac gttaaagaat ataaatacgt tgaagaatat gtacgtaaaa ccgctgatgc
      241 attaattaag aagagtgggc aaaaacttca ttacctcgtt ggtacaatga ttgaaatccc
      301 acgagcaacc ttacttgccg accaaattgc taaagaagcc gaattcttca gctttggaac
      361 aaacgactta acgcaaatga catttggctt tagccgtgat gatgctacga agtttattcc
      421 tgactatact gaagcacgga tcttcgaaca agacccattc caaaccttag acactgaagg
      481 cgttggccaa ttagtgcgct taggtgttga acgcggtcgt aagggcaacc ctgatgttca
      541 cttaggaatc tgtggtgagc atggtggtga tccaaactct gtcgaattct ttgatagcgt
      601 tggcttagac tatgtcagct gttcaccata tcgtgtgcca attgctcgct tagccgcagc
      661 ccaagctgca atcaaacata aaaaagctaa gtaataatta ctattaagat agctttaacc
      721 tcgttcttcg gagcgaggtt ttttattatt taaaaagacg ctcaatacaa gcgtctttcg
      781 ttaatttctc agcaatttct atttcttttt ttgtttttca cgaattattt tcgcatagcc
      841 ttctttattt tcttggcgtg aacgcgcgat agcaagtgtg tcttttggta catctttatt
      901 aatgatcgat gcagcggcaa gataagagcc ttccccaatt tgaaggggtg ctactagagt
      961 cgtgtttgtg ccaataaaga cattttcacc cacttttgtt tttgatttat ttacgccatc
     1021 ataatttgca gtaattgctc ctgcaccaac attagtgtgt gcgcctaatt cagcatctcc
     1081 catatacgct aagtggccag ctttaacgcc ttcgtggaat gttgtatttt taagttcgac
     1141 aaagttccca atatgtgagc cgtcttttac caaagctttt tgtcgcattc tcgcaaacgg
     1201 accaatcgtg acattgttac aaatttctga gtctactaag taactataaa taatagaatt
     1261 ataatcgcct atcttcatat tctttaaata tgtgtttggt ccaatttcat ttgcaacacc
     1321 aatcgtgcta ttgcctaata tcgtagtgtt aggactaata attgtatctt ggccaattgt
     1381 gacgtctggt cctatatatg tgttatttgg atcttcaagt gtaacgccgt ttagcatatg
     1441 tgctttatta atgcgttttt taaggagttc cgtagcttcg gcaagttgcg ctctatcatt
     1501 tgtgccaagc atttcatcag gatcacttaa cacaaaggca ccaactttat accctctttc
     1561 tttaaataaa ccgataatat ctgttaaata taattcgcct tgtttattgt ttggttttaa
     1621 gtttttaagt tcttggaata acttttggtt attaacgaca tacatcccag cattaacttc
     1681 actaatgtcg ccttcattac ctgttaagtc tttttgttca acaatgcgag ttacttcacc
     1741 gtttggtgcg cgtaaaactc taccataacc aaaaggatta tctggcttag cggttaatat
     1801 tgttaaatcg tttttctctt tggtgtgaag attaagtagt ccgctgatgg agtgttcagt
     1861 aataagtggt acgtctccac ttaaaattaa agtgtcaccc ttttcctttt caagtagcgg
     1921 tgctgtttga atgattgcgt gtcctgtacc tttttgttca tgttgccaag caatttcagc
     1981 tttcccttcc acgagtttag aagtgtgttc accaccaaaa ccgacaatca caacagttct
     2041 tcctgttaca tgcggctttg cggcatctaa cacccaatta ataagtggct tgcctaatat
     2101 ttcaaaacct actttcgata tttcttgatt taatgatttc atgcgggtgc ctttgcccgc
     2161 cgctaaaata atggcgtttc ttttcataaa attctcctat ctgatgactt ttgtcaatac
     2221 tgtttgataa ttttgtttgc gatatttatc gctaataaaa cgtaataagt gtcgattatc
     2281 acttagtgca aaaatcgtag tacctgttcc cgctatacca taagcttcaa catttaattg
     2341 ttttagatca ttaagcactg tccgtaatgc tggtgtatgt ttaataatcg tttcactaaa
     2401 gtcattaaaa agcacagaac ctaactcacg caaagaacca ctttctgcaa gttcaacaac
     2461 tttttcaagc ggtttttgta gtgattcacc ataatccaaa aagtctttaa caacattttc
     2521 tttttcataa atgtctgggt ggataattaa taacacctgt ggattatgtt taaattttat
     2581 aggtgttact tcttgtttgt ttaaattgta cactgcgggt ttatttacta aaaaataagg
     2641 gacatctttt ccaattttga ctgcaagatt ataatattgc tctggtgttg gttttagttt
     2701 agcaaggtgt gcaacaactt tcacgacagc ggtcgcatca cttgccccgc tccctaaacc
     2761 gtgtcctgtt ggaatatttt tatggatttt aacagagaag ttttgcttat aaccaaattg
     2821 ttcccgcatt tttagtaacg cttcatcaat aatccgtttt tctttaacat gaagcgcctt
     2881 attagtgtaa tgaacaatat ccataaaggc agtgttgaag acatcaatcg taattgtgtc
     2941 actaaattca ataggtaaaa caaggctttt aatctctaaa gcgttagtca atggattaag
     3001 cgttgtttct acacttatgg acaagcgtgc attcgcagtt actatcatat ttatatgata
     3061 tcaaaattac attgttttta gggcgttttc agtgcttata ataaactaac agtataaatc
     3121 cctgtgtgta tctcataaga aaacactcct ttcctattgt ctaggatttg gggtgcatct
     3181 caaaattatc ttttttcttt tgttaacaat gtagataagt atttgtcctg ataccctaag
     3241 cgtagacact ttaataatga aagaccagga atttgacttc ctggtctgtc caccttggat
     3301 ttaatagttt tagggtgtta atttggtttt ccaccttttt ccaccttaga tttaatagat
     3361 ccaaataatt taaaaatata atggcctttt agctaaatat gagatgttca agttcgcata
     3421 atcagtatca taattataat agtctagttc catggtttca tttgacatca ggttaatata
     3481 tttatcttct gtttttatcg cgggtatata accccctatt tttccgttgt ttgcttttaa
     3541 aaaatattat ttattgttag aagtgtcaca attttgatat ggattaaact g
//
LOCUS       DAOPCD010000089         3581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_21035_length_3581_cov_3.429382, whole genome shotgun sequence.
ACCESSION   DAOPCD010000089 DAOPCD010000000
VERSION     DAOPCD010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21035_length_3581_cov_3.429382"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PLQ55_02915"
     CDS             complement(<1..535)
                     /locus_tag="PLQ55_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28914.1"
                     /translation="MKKKTIILMIPLLIFTLVVSGCERGSESLSDNSRPTLGSEEPTS
                     TTITSTEGRTGQKELSFYATNDFHGSIVETNSEPGIFKLGSYLKSEFAAKRNKTVYVD
                     AGDIWQGSADSNINRGKFLVEAMNVLKLDAQTIGNHEFDWYDTVIETNKELANYPFLG
                     ANIMVRETGELATNLVSY"
     gene            complement(532..>925)
                     /locus_tag="PLQ55_02920"
     CDS             complement(532..>925)
                     /locus_tag="PLQ55_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPK28915.1"
                     /translation="GGGAYELFADGSTKKLSVNDKTSNLTATRSRHHLKPADNEYIEK
                     HKDLISNVITYGSTLKAIKIAKGEIELFYRSGPGTKEWDIAAADIIVTEAGGIFIGPD
                     GKKFTYNKADIHNRNGYLIVNRKENIRL"
     assembly_gap    926..935
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<936..1315)
                     /locus_tag="PLQ55_02925"
     CDS             complement(<936..1315)
                     /locus_tag="PLQ55_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPK28916.1"
                     /translation="MKYKKELNAAIEAGKIARDIVLKIYESDFDVEIKDDNSPVTLAD
                     KMADKAMREYLGPLFPDYAFLTEESDDDLSRFTTDYVWIIDPVDGTKDFIHRDGEFTV
                     CIALTYKGEVVVGVIVVPVLNEIY"
     gene            complement(1305..2246)
                     /locus_tag="PLQ55_02930"
     CDS             complement(1305..2246)
                     /locus_tag="PLQ55_02930"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPK28917.1"
                     /translation="MFKNLRKFIEEHDKIAIFSHIYPDGDAVGSIIGLRELIKTSYPH
                     KEVFGLGSNVQPFVSIVGELDEVSDDFIKESAAIIVDVANGERVEDQRFKLAKASFKI
                     DHHIFVEKFTDEELVLTKRIATAEIIGELMMKEKLRTNKLGATALALGIITDSGRFRY
                     DLTSGNTFSVMARLTNIGADLALINENLAKRSLADFKKRGHFLSNYETYENVIYIIVP
                     YLKLQELDILPSEGSNYVNTYSNLDDYPLWATFFIEKDGRVFVELRSKNYNVQKVATL
                     FGGGGHLKASGCRLENEARIADVLQQLTKAEELKDEV"
     gene            complement(2247..3104)
                     /gene="fba"
                     /locus_tag="PLQ55_02935"
     CDS             complement(2247..3104)
                     /gene="fba"
                     /locus_tag="PLQ55_02935"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HPK28918.1"
                     /translation="MPLVSMKEMLIKANKEKYAVGQFNINNLEWTQAILDEVQELQAP
                     VILGVSEGAAKYMGGYNVVVQMVKALIKDRGYTIPIAIHLDHGSSEEVCKKAIDAGFT
                     SVMIDASHHPFKENLEITKRVVDYAHARGVSVESELGSIGGQEEHVIAETLYADPNEC
                     LEMVQKTKIDCLAPALGSVHGPYKGEPKLGFDEMDHINKLVGVPLVLHGGSGIPDFQI
                     QMAIERGTAKINVNTEIQQEWTKIVRETLANNDKVYDPRKIIGPGMKGIRKVVNEKCR
                     VFGCIGKAA"
     gene            complement(3134..3520)
                     /gene="rpoE"
                     /locus_tag="PLQ55_02940"
     CDS             complement(3134..3520)
                     /gene="rpoE"
                     /locus_tag="PLQ55_02940"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR04567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HPK28919.1"
                     /translation="MKKRTNIEIAYTLLKEHGESLSFYDLWQGVVNEHEYSEAEGHDL
                     ISRFYTALTMDGRFVNLGDNMWALRENVLFDDIALPLNEVYTEVDEAGDEEEEDEGPE
                     ILYFDDETGQADEAKVRSTLDEEDEE"
BASE COUNT         1064 a          788 c          528 g         1191 t
ORIGIN      
        1 cgtatgacac taaattcgtg gcaagctcac cggtttcacg caccatgata ttagcgccta
       61 aaaacggata attagcgagt tccttattcg tttcaataac agtgtcatac caatcaaatt
      121 cgtggttacc aatagtttgc gcatcaagct ttaaaacatt cattgcttca actaaaaact
      181 taccacgatt aatatttgaa tcagcactgc cttgccagat gtcaccagca tcaacataca
      241 ctgttttatt tcttttagca gcgaattcac tttttaaata tgaaccaagt ttaaaaatac
      301 caggctcact atttgtttca acaattgacc catgaaaatc attagtagcg taaaaactta
      361 attccttttg cccagttcta ccttcagtgc ttgtaattgt agtgctagta ggttcttcac
      421 tacctaaggt aggtctagaa ttgtcactta gagattcact tcctctttca catccgctta
      481 ctacaagcgt aaaaataagg agcggtatca ttaaaataat tgtttttttc ttcataatcg
      541 tatattttcc ttccgattaa cgattaaata accgttacga ttgtgtatat cagctttatt
      601 ataagtaaac ttcttaccat caggtccaat aaaaataccg ccagcttcag tcacaatgat
      661 atccgctgca gcgatatccc attctttagt gcctggacca ctacgataaa ataattcaat
      721 ttcaccttta gcaatcttaa tcgctttaag tgtggagccg tatgttatta cattactaat
      781 taaatcttta tgtttttcga tatattcatt atcagcaggt tttaaatgat gacgagaacg
      841 agtcgcggtt aagttactag ttttatcatt aacacttaac ttctttgtcg aaccatcagc
      901 gaatagttcg taggctcctc cgcctnnnnn nnnnntaata tatttcgtta agtaccggca
      961 caacaatgac accgacaaca acttcacctt tataagttaa agcgatacac acagtaaatt
     1021 caccatcacg atgaataaaa tcttttgtgc cgtcgaccgg atcaataatc cacacataat
     1081 ccgtggtaaa ccgagataaa tcatcatcac tttcttcagt taaaaaagca taatcaggaa
     1141 aaagtggtcc aagatactca cgcattgctt tatccgccat tttatcggct aaagtaacag
     1201 gagaattatc atctttaatt tcaacatcaa agtcactttc ataaattttt aaaacaatat
     1261 cacgcgctat tttcccggct tcaatcgccg catttaattc ctttttatac ttcatctttt
     1321 aactcctcag ctttagtaag ttgttgcaaa acgtcggcaa ttcgtgcttc attttctaag
     1381 cgacagccac ttgcttttaa gtgaccaccg ccaccaaaaa gcgtagcgac cttttgcaca
     1441 ttataatttt tactacgtaa ttcaacaaac acacgcccgt ctttttcgat aaaaaaagtt
     1501 gcccataacg gatagtcatc gagatttgaa taagtattga cataattact accttcactt
     1561 ggtaatatgt ctaattcttg tagttttaag tatgggacaa taatataaat tacattttca
     1621 taagtttcat aatttgataa aaagtgtccg cgttttttaa agtcagcgag tgaacgcttc
     1681 gctaaattct cattaattaa cgctaaatcc gcaccaatat tagtgagtcg cgccatcaca
     1741 ctaaaagtgt taccgcttgt taagtcgtat ctaaaacgac ctgaatcggt aataatccca
     1801 agcgctaagg cagtcgcacc aagtttattt gtgcgaagct tttctttcat cattaattca
     1861 ccaataattt cggcagtagc aatacgcttt gttaacacta actcttcatc agtaaacttt
     1921 tccacgaaaa tatgatgatc aattttaaat gatgcttttg ctaatttaaa gcgttgatcc
     1981 tcaactctct caccgttagc gacatcgaca ataatcgccg cgctttcttt aatgaagtcg
     2041 tcacttactt catctaactc gcccacaata gaaacaaagg gttggacatt actgcccaac
     2101 ccaaaaactt ctttatgcgg ataacttgtt tttattaatt cacgcaagcc aataatactc
     2161 ccaactgcat cgccatcagg ataaatgtgt gaaaagattg caatcttatc atgctcttca
     2221 atgaacttac gtaaattttt aaacacttat gcagctttcc ctatacaacc aaagacgcgg
     2281 cacttttcgt taacaacttt acgaatgcct ttcattcccg gaccaataat tttacgtgga
     2341 tcataaactt tatcattatt cgctaaagtt tcacggacaa tctttgtcca ttcttgttga
     2401 atttcagtgt tgacattaat tttagcggta ccacgttcaa tcgccatttg aatttggaaa
     2461 tcaggaatac cactgccacc atgtagcaca agtgggactc cgactaattt attaatatga
     2521 tccatttcat caaaaccaag ttttggttcg cctttatacg gaccatgtac actaccaagc
     2581 gctggcgcta agcagtcaat ttttgttttt tgcaccattt ctaaacattc atttggatca
     2641 gcatataatg tttcagcaat aacgtgttct tcttgaccac cgatgctacc aagttctgat
     2701 tcaacactaa cgccgcgtgc atgtgcgtaa tcaactacgc gtttagtaat ttctaaattt
     2761 tctttaaatg ggtgatgact tgcgtcaatc atcacggaag taaagccagc atcaattgcc
     2821 tttttacata cttcttcact gctgccgtga tctaagtgga tggcaattgg aatcgtatag
     2881 ccacgatctt taattaaggc ttttaccatt tgcacgacga cattataacc gcccatgtat
     2941 ttagcggctc cttcactaac tcctaaaata actggagctt gaagctcttg tacctcatcg
     3001 agaattgctt gggtccattc taagttatta atgttgaatt gaccaacagc gtacttctct
     3061 ttgtttgctt tgataagcat ttctttcata ctcactaatg gcataaaaat tctcctttat
     3121 cttttaaaat tatttattct tcgtcttctt cgtcaagtgt gcttcttact ttcgcttcat
     3181 ctgcttgacc tgtttcatca tcaaagtaaa ggatttctgg tccttcgtct tcttcttcct
     3241 catcacctgc ttcatcaact tcagtgtaaa cttcgtttag tggtaaagca atgtcatcaa
     3301 ataagacatt ttcacgtaat gcccacatat tgtcgcctaa attaacaaag cgaccatcca
     3361 tcgttaaggc agtgtagaag cgtgaaatta aatcatgacc ttcagcttca ctatattcat
     3421 gttcgttaac aacaccttgc cataaatcat aaaatgatag tgattcaccg tgttccttaa
     3481 gtagggtgta agcaatttca atgtttgtcc ttttcttcat atatcttctt gtattatcta
     3541 atcatgcccc ggtacttcga ttaggtaata catctccttt c
//
LOCUS       DAOPCD010000090         3560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_21153_length_3560_cov_4.718973, whole genome shotgun sequence.
ACCESSION   DAOPCD010000090 DAOPCD010000000
VERSION     DAOPCD010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21153_length_3560_cov_4.718973"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /gene="rplA"
                     /locus_tag="PLQ55_02945"
     CDS             complement(<1..627)
                     /gene="rplA"
                     /locus_tag="PLQ55_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPK28920.1"
                     /translation="MKRGKKYKAAAALVDKEKLYTIEEAVALVKQTSTTKFDATIDVS
                     FRLNVDPRHADQQIRGTLVLPHGNGKEKTVLVITNSPEAAKEAGADFVGGKDMIEKIK
                     SENWFGYDVIVATPDMMGELGKLGRLLGPKGLMPNPKTGTVTTDVAKAVQEIKAGKVE
                     YRVDREANMHVSIGRVSFEDEKLIDNLNALTERIVRVRPAAVKGTFIKN"
     gene            complement(668..1084)
                     /gene="rplK"
                     /locus_tag="PLQ55_02950"
     CDS             complement(668..1084)
                     /gene="rplK"
                     /locus_tag="PLQ55_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPK28921.1"
                     /translation="MSKKIAKVVKLELIGGEAKPGPKLASVGVIMPKFCTDFNAQTGD
                     RRGEVVPVIITAYEDKSHSFILRTTPTSILLRKAAGDIKKGAGNPKVEKVGKITQTQL
                     REIAEYKLPDLNANDVEAAMKIVAGTARNMGIVVED"
     gene            complement(1350..1904)
                     /locus_tag="PLQ55_02955"
     CDS             complement(1350..1904)
                     /locus_tag="PLQ55_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPK28922.1"
                     /translation="MAENMEMKWYVASTQSGQENTVVHNIRRRLESSDKADTVARIIV
                     AEIEEEVLNKDNKIVIKKRNLYPGYIFLEMEMSDEMWFLIRNTPGVTGFIGSSGRGAK
                     PFPVEYEELEPILKRLGHVDKTMYERYAVDDYVRVIDGPLVGTEGRIIEIDRETGNVK
                     IEAVFFGRVTTADINFALIEKVEF"
     gene            complement(1913..2113)
                     /gene="secE"
                     /locus_tag="PLQ55_02960"
     CDS             complement(1913..2113)
                     /gene="secE"
                     /locus_tag="PLQ55_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPK28923.1"
                     /translation="MGLRKYSNEVVKEAKRVRWPKPDVLFPTIVVVLVIVFFAAFMLF
                     VEDLAVGKLLGEIERVFQGFKK"
     gene            complement(2124..2273)
                     /gene="rpmG"
                     /locus_tag="PLQ55_02965"
     CDS             complement(2124..2273)
                     /gene="rpmG"
                     /locus_tag="PLQ55_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004028373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPK28924.1"
                     /translation="MRKKILLICEVCLSRNYNTSVNKATQTERFQIKKYCPKCNKMTL
                     HKESR"
     gene            complement(2367..3446)
                     /locus_tag="PLQ55_02970"
     CDS             complement(2367..3446)
                     /locus_tag="PLQ55_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPK28925.1"
                     /translation="MKKDPFIEYLKENNPTEKQKKYSWYTAIGLQEVDGLETSDYLKE
                     TAIKNIKGEISLDETKRLIFAYYEHATEREKQNEEADKVSIQIAEILSKKAFTFSVVE
                     FLKTHKFLFLDIYEHAGEIRKFNISKKEWILNGASVTYGDFSSLKDMLEYDISQEKSV
                     DYSKFSRDERIKHIAKFIADLWQIHPFAEGNTRTTAVFLIKYLRSLGFDVTNDLFAKN
                     SWYFRNALVRANYNNVSKEIFETTEYLELFLRNLLYNENNKLSNRQMHVEYMKKPDID
                     KEKPDIDKEKPDIALKRMSLTVKTKANIMRLNDSIKNDIFGRKEIMHILGLSASRSSE
                     LIKQMLDCDIIVAVKGQGKGKYIFK"
BASE COUNT          986 a          719 c          592 g         1263 t
ORIGIN      
        1 atttttgata aatgttcctt taactgcagc gggacgaact ctcacaatgc gttcggtaag
       61 tgcgttaagg ttatcaatta atttttcgtc ttcgaagctc acgcggccaa ttgacacgtg
      121 catatttgct tcccggtcaa cacgatattc gactttacca gccttaattt cttggacggc
      181 tttagcgaca tcagtcgtga cggttccggt ttttgggtta ggcataagtc cttttggacc
      241 gagtaatcgt cctaatttcc caagttcgcc catcatatct ggggtggcga cgataacatc
      301 ataaccaaac cagttttcgg acttaatttt ttcaatcatg tctttacccc cgacgaagtc
      361 agcgcctgct tctttagcgg cttctggact attagtaatg acaagaactg ttttttcctt
      421 accattaccg tgtggcaaca cgagtgttcc cctgatttgt tggtcggcat gacgtgggtc
      481 aacatttagt ctaaatgaga catcgatagt ggcatcaaat ttagttgttg aagtttgttt
      541 gacaagcgca actgcttctt cgatcgtata aagtttttct ttatcgacta aagccgctgc
      601 ggctttgtac tttttccctc ttttcatatt tcctcctctg tggtactaac gagtgggttc
      661 tcttccacta atcttcgact acaatgccca tgttacgtgc ggtaccagca acgattttca
      721 ttgcggcttc gacgtcatta gcattgaggt caggtaattt gtattcggca atttcgcgta
      781 attgggtttg ggtaattttc ccaacttttt cgactttagg attgcctgct ccttttttga
      841 tgtccccagc ggctttccgt aaaagaatgc tggtgggtgt cgttcttaaa atgaaagaat
      901 gcgacttgtc ttcatatgcc gtaataatta ctggaactac ttcaccgcga cgatcaccgg
      961 tttgggcatt aaagtcagta caaaatttag gcataataac acctactgac gctaattttg
     1021 gaccaggttt agcttcgcca ccgataagtt cgagttttac gacttttgcg atttttttac
     1081 tcacgtgact ttcctccttc gttttttaga tatgtccgtg ctgtggtaag gggaagatcg
     1141 cctcttcctc ccacgagcgt attatgtccg cacatcgcga gcaatatacg catatgatta
     1201 taccaaaaaa atatttttgg gcaacgataa attgtaattt ttcgtgtttt atgttaaaaa
     1261 cagcatttta tgactatcat tttatgataa tttgattaaa aaacacccca gcacaatgct
     1321 ggggtgaatg tttttagagt tgtttttatc taaaattcaa ctttttcaat taacgcaaag
     1381 ttgatatcag cggtggtaac gcgaccaaag aagactgctt caattttaac atttcctgtt
     1441 tcgcgatcaa tctcaatgat ccgtccttca gtgcccacga gtggtccgtc gataacacgg
     1501 acataatcat ccactgcgta acgttcatac attgttttat caacatgacc taaacgtttt
     1561 aagattggtt caagttcttc gtattcaaca gggaaaggct tcgccccacg acctgatgaa
     1621 ccaataaaac cagtaacacc aggagtatta cgaataagga accacatctc atcgctcatt
     1681 tccatttcta agaaaatgta acccggatat aagttccgtt ttttaataac aattttattg
     1741 tctttattta atacttcttc ttcaatttcg gctacgatga tgcgcgcaac agtgtcagct
     1801 ttatcacttg attcaagccg ccgacggatg ttatgtacaa ccgtgttttc ttgtcctgat
     1861 tgcgtacttg caacgtacca tttcatttcc atattttctg ccataacgtt acctacttct
     1921 taaatccttg gaagacgcgt tcaatttcac cgagtaattt accgaccgct aggtcttcta
     1981 caaataacat aaaagcagca aaaaagacaa taactaacac aacgacaatt gttgggaaaa
     2041 gcacatctgg ttttggccaa cggacacgct ttgcctcttt aacaacttcg tttgaatact
     2101 ttcttaatcc cataatcttt ctcctaccga ctttccttgt gtaatgtcat tttattacac
     2161 tttggacaat attttttaat ttggaagcgt tctgtttgcg tggctttgtt gacggatgtg
     2221 ttgtaattcc gtgacaagca aacttcgcat attaaaagaa tctttttccg catatattat
     2281 actcctttta agtgtatagt aaaggaataa tagcacagcc attacttcct gtcaaagagt
     2341 atagcataat tccacgaagg tttatctcac ttgaaaatat atttaccttt accttgacct
     2401 tttactgcaa caataatgtc acaatctaac atttgtttta taagttccga ggatctagat
     2461 gcagacaaac ccaaaatgtg cattatttct tttctcccaa aaatatcatt cttaattgag
     2521 tcattcagtc tcataatatt agccttagtt tttacagtca gagacattct ttttaaggca
     2581 atgtccggtt tttctttatc aatgtccggt ttttctttat caatgtccgg ttttttcata
     2641 tattccacat gcatttgtcg atttgaaagt ttattgtttt cgttatacaa aaggttcctt
     2701 agaaataact ccaaatactc tgtagtttca aatatttctt ttgaaacatt attataattt
     2761 gctcttacta aagcgttccg aaaataccat gagtttttag caaataaatc attggtgaca
     2821 tcaaacccca aagaacgcaa atactttatt aaaaatactg ccgtcgttct tgtattgccc
     2881 tcagcaaaag ggtggatttg ccataaatca gcaataaatt tagctatgtg tttaattctc
     2941 tcatctctcg agaacttaga ataatctaca cttttttctt gacttatatc gtactccaac
     3001 atgtctttta aggaagaaaa gtctccataa gtaacacttg ctccatttaa aatccattcc
     3061 tttttactaa tattgaattt acgaatttca cctgcatgtt cataaatgtc caaaaataga
     3121 aatttgtgag tttttaagaa ctcaacaaca gaaaaagtaa aggccttttt ggataaaatt
     3181 tctgcgattt gaattgaaac tttgtcagct tcttcgttct gtttttctct ttcagtagcg
     3241 tgttcataat atgcgaaaat taatctttta gtttcatcta aagatatttc gccctttata
     3301 ttcttgattg cggtttcttt caaataatcc gaggtttcta atccatcaac ttcttgtaaa
     3361 cctatcgcgg tataccaaga gtatttcttt tgcttttcgg tagggttatt ttcttttaaa
     3421 tattcaatga atgggtcttt tttcataaat tattccttat ttattatacc atatggaaag
     3481 cgatttatta agctttgtaa ttaataattt ttgccgatta tatggagtaa aacaaaattc
     3541 taaattttat aaaagtagtg
//
LOCUS       DAOPCD010000091         3504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_21564_length_3504_cov_3.861699, whole genome shotgun sequence.
ACCESSION   DAOPCD010000091 DAOPCD010000000
VERSION     DAOPCD010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21564_length_3504_cov_3.861699"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLQ55_02975"
     CDS             <1..315
                     /locus_tag="PLQ55_02975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPK28926.1"
                     /translation="PLVEELTYRVGLFNAISSRNRIAAYVVSALVFGFLHFNIPLNAD
                     GAIDQAKLIEEFINIPSYIVSGLVFGYIYEKEGFAGSSVAHITNNLISYILSIISIYA
                     SK"
     gene            302..1132
                     /locus_tag="PLQ55_02980"
     CDS             302..1132
                     /locus_tag="PLQ55_02980"
                     /inference="COORDINATES: protein motif:HMM:NF017655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HPK28927.1"
                     /translation="MQANRHLILNSFTLKIIAMSAVLIDHVALLFINPTLTIYILMRI
                     IGRIAFVLYAFFISEGVIHTKNTNKYLLRILYLYLIIQALIIGVLIYDPVIELRNIFA
                     TLGAGASLLIYFEKKEWKKVYYLLPFIIVSTINLLAYFTKDSWFIAFSGDNGFFGLAL
                     IIAFYVARKFSIYLLKKYPPSDNTAGEDAEKSAQHVYNVASCVSLLFVASIWYILSVY
                     NSEIPHVALQSFSLISIPLLLLYNGKLGHNSRSWRIIYYSFYPVHVVMLALLFFIIKL
                     "
     gene            1142..1450
                     /gene="trxA"
                     /locus_tag="PLQ55_02985"
     CDS             1142..1450
                     /gene="trxA"
                     /locus_tag="PLQ55_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009872735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPK28928.1"
                     /translation="MIKYLENAADFKEIISKGTVLVDFYADWCGPCRMLGPVLEKLSK
                     ERTDVTVLKVDVDTFGALSQQFSVFSIPTMILFKDGKPVGKQVGYKAFSQLVDFIDNN
                     "
     gene            1511..3358
                     /locus_tag="PLQ55_02990"
     CDS             1511..3358
                     /locus_tag="PLQ55_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28929.1"
                     /translation="MSKLIFDPLEEYRDKFKGLHDQNVNRFFDELVQKSGINIPENDL
                     TVKEIDNLNIKIDNEEKVLRRVRRIRGFLLFLFIAALIVFILFIVAAVQGEESSLTMS
                     VGARVGIAIGVIVFGIAMLIIVIKVLRPRIKDSQARLSDLISKRDEKIRLAWAQVNPL
                     NELFDANMTPMLVEKTIPLLTMDPFFDSKRLDYLVRKYELSDFTPDNESAEFVQSGEI
                     QGNPFILARTLVMNMGTKTYTGTLTVSYTVTVYVNGKAQRQTRTETLRASVNKPCPYY
                     NNDTYIIYGNEAAPNLIFSRYPQLPLDWTEKSLRRFVEKEEKEMKKLSEKALKQGKQF
                     TPLGNTEFEALFNAYDRNHELEYRLLFTALGQSEILDLIKDKKVGFGDNFQFFKNKNL
                     NLVRSKHSQAFDYSGNPRNYYHYDNKEIRKRFVDYNNNYLHRFYFNIAPVLAIPLYQQ
                     HKPHEFIYQTEYKSHLSFYEHESTVNQFPSSAFAHPDSHTQNILKTKVVQKGKDFDTV
                     SVTSYGFTTVERVDYVPKMAGNGTIHNVPVHWTEYIPVEKESHATIKVLDEESVKKTQ
                     NESFFDKIGEYLSKYTPDQKPVTGRHVMAFLVASKFADDDNEGLDKLFK"
     gene            3405..>3504
                     /locus_tag="PLQ55_02995"
     CDS             3405..>3504
                     /locus_tag="PLQ55_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPK28930.1"
                     /translation="MANELDELTGPVNEEGQDINVISKQLKVKVGWG"
BASE COUNT         1140 a          590 c          627 g         1147 t
ORIGIN      
        1 ccactagtgg aagagttaac gtatcgcgtt gggttattta atgcgatatc gtcacggaat
       61 cgtatcgcag cctacgtcgt tagcgcactt gtgtttggtt tcttacactt taatattcca
      121 cttaacgctg atggcgctat tgatcaagct aaactaattg aagaatttat taatattcct
      181 agttacattg ttagcggact cgtctttggc tatatttatg aaaaagaagg gtttgctggg
      241 tcaagtgttg cccatatcac taacaattta atatcgtata ttctttcaat tattagtatt
      301 tatgcaagca aatagacatt taatattaaa ttcattcacg cttaaaatta tcgcaatgag
      361 tgcagtttta attgaccatg ttgcgcttct ttttattaat cccacattaa caatttatat
      421 cctaatgcgc attattgggc gcattgcatt tgtactttac gcttttttta taagtgaagg
      481 agtgatacat actaaaaata ccaataaata cttattgcgc attttatatt tatatttaat
      541 aatccaagca cttatcattg gcgttttaat ttacgaccca gttatcgaac taagaaatat
      601 tttcgctacg cttggcgctg gtgcaagttt actcatctat ttcgagaaaa aagaatggaa
      661 gaaagtgtat tacttattac cgtttattat tgtttcgaca attaatttat tagcctattt
      721 tactaaagat tcgtggttta ttgctttttc cggggataac ggcttctttg gtttagcctt
      781 aatcatcgct ttctatgtcg cgcgtaaatt cagtatctat ttactgaaga agtatccgcc
      841 tagtgataat accgctggag aggatgctga aaaaagcgca cagcatgtgt ataatgtcgc
      901 aagttgcgtt tctcttttat tcgttgcgtc catttggtat attttaagcg tgtacaatag
      961 cgaaataccg catgttgcat tgcaaagttt ctcacttata agtattccgc tcttgttact
     1021 ctataacggt aaattaggcc ataatagtcg tagttggcga attatctatt attctttcta
     1081 tcccgttcat gtagtaatgc ttgcactact attcttcatt attaaactct aggaggcact
     1141 tatgattaaa tacttggaaa atgcagcaga ttttaaagaa attattagta aaggaacagt
     1201 cttagtcgac ttttacgctg attggtgtgg tccgtgccga atgcttggtc cagtccttga
     1261 aaaattaagt aaggaacgca ctgatgtcac tgttttaaag gttgatgttg acacttttgg
     1321 agcactctcc caacaattta gtgttttttc gattccaaca atgattcttt ttaaagatgg
     1381 caaaccggtt ggtaaacaag ttggttataa agccttttca caattagtgg actttattga
     1441 taataactaa aatttgaaat ttttatcata aaataaatta caatttttta atctttgtta
     1501 taatcaatct atgagcaaat taatttttga cccacttgaa gaatatcgtg acaagtttaa
     1561 agggttacat gaccaaaatg taaaccgttt ttttgatgaa ctagtgcaaa aaagcggcat
     1621 taatattcct gagaacgatt taaccgttaa agaaatcgat aatttaaata tcaaaatcga
     1681 taatgaagag aaagtactcc gtcgcgtacg gcgtatacgc gggtttctct tatttttatt
     1741 tatcgccgct ttaatagttt ttattttatt tattgttgcc gcagtgcaag gtgaagagag
     1801 ttccctaacg atgagcgttg gtgcacgagt agggattgcc attggcgtaa tcgtatttgg
     1861 tatcgctatg ctcataattg tcatcaaagt attacgacca cggattaaag attcacaagc
     1921 tagactaagt gacttaatta gtaagcgtga cgaaaaaatt cgcctagcat gggcacaagt
     1981 taatccgctt aatgaactgt ttgacgcaaa tatgacacca atgttagtgg aaaaaacaat
     2041 tccgctgctt acaatggatc cattttttga ttccaagcgg cttgattact tagtgcgtaa
     2101 atacgaatta agtgacttta ctcctgataa tgaatccgcg gaatttgtgc aatcgggtga
     2161 aattcaaggt aatccattta ttttagcaag aacgcttgtg atgaatatgg gtactaaaac
     2221 ttatactggg acacttactg tttcttatac ggttaccgtc tatgttaacg gtaaagcgca
     2281 acgacaaaca cgcacagaaa cattgcgcgc aagtgtgaat aaaccgtgtc catactacaa
     2341 caatgatact tatattattt atggtaacga agcagcgcct aatttaatct ttagtcgtta
     2401 tccgcaactc ccacttgatt ggacagaaaa gagtttacga cgctttgtcg aaaaagaaga
     2461 aaaagagatg aaaaaactta gtgaaaaagc actaaaacaa ggtaaacaat ttacaccact
     2521 tggtaatact gaatttgaag cactctttaa cgcttatgac cgcaaccatg aattagaata
     2581 tcgcttatta tttactgctt taggtcaaag tgaaatatta gatttaatta aggataagaa
     2641 agttgggttt ggtgataact tccaattctt taaaaacaaa aacttaaatc ttgttcgttc
     2701 taaacattca caagcctttg attatagtgg gaatccacgt aattattatc actatgataa
     2761 taaagaaatt agaaaacgct ttgtcgatta taacaataat tatttgcatc gtttctattt
     2821 taatatcgct cctgtccttg caatcccgct ttatcaacaa cataaaccac atgaatttat
     2881 ttatcaaaca gaatataaat cgcacctttc attttatgaa catgaatcaa cggtgaacca
     2941 gttcccaagt agtgctttcg cgcaccctga tagtcataca caaaacatct taaaaacaaa
     3001 agttgtgcaa aaaggtaaag acttcgatac cgtcagtgtg actagctacg gttttactac
     3061 tgtagaacgc gttgactatg ttcccaaaat ggcaggaaat ggcactattc ataatgtccc
     3121 tgtccattgg actgaatata tcccagttga aaaagaatca cacgcaacga ttaaagtact
     3181 tgatgaagag agcgtgaaga aaacacaaaa tgaatcattc tttgataaga ttggtgaata
     3241 tttaagtaaa tacacccctg atcaaaaacc ggtcaccggc cgccatgtta tggctttcct
     3301 tgtggcaagt aaattcgctg atgacgataa tgaaggcctt gacaaattat ttaagtaata
     3361 agtttattta ttaatagtat aattaaagaa aggagaaaaa tactatggca aatgaattag
     3421 atgaactaac tggaccagtt aatgaagaag gtcaagatat taatgtaatt agtaaacaac
     3481 taaaagtaaa agttggatgg ggtt
//
LOCUS       DAOPCD010000092         3487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_21681_length_3487_cov_3.016026, whole genome shotgun sequence.
ACCESSION   DAOPCD010000092 DAOPCD010000000
VERSION     DAOPCD010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21681_length_3487_cov_3.016026"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..348
                     /locus_tag="PLQ55_03000"
     CDS             181..348
                     /locus_tag="PLQ55_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28931.1"
                     /translation="MNKNRKWLARSAVISLLISAIITPAFFRKDAVKTKAATTIPVLV
                     GDSIGWEPSVS"
     gene            451..846
                     /locus_tag="PLQ55_03005"
     CDS             451..846
                     /locus_tag="PLQ55_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28932.1"
                     /translation="MEDLQFDTTPSLSRDYIYRYEDSPDANFITIYPGEIKVQVFNPD
                     NAGGSPAGARRMKVEYTSLLGATPSTPTGTSSYALRGPAVGDWTTHDINNCLVGISGY
                     DGRYLSLTRAFNADDFKIFVYKQTLNEPY"
     gene            898..1548
                     /locus_tag="PLQ55_03010"
     CDS             898..1548
                     /locus_tag="PLQ55_03010"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HPK28933.1"
                     /translation="MPGDPSSGYHESFHVNTAGNYMVVYDLKMQKIYFHNAGTTYHKV
                     TKYYGELLIEEAAAFTGLNYTPAPFYQPEKKFVGWYEDALLETAYVPKDLTSDITLYA
                     KFVDSPSVNLRFYDRNLDIKVDNGGFNEYVVFAYGKNTDGTEPLGGWPGRMMQKVVDM
                     GHELSYGYYALNISGQDRPEKVTFNNGCQPFEPEHVSTPELVLPADKNLYEHDVAA"
     gene            1555..1953
                     /locus_tag="PLQ55_03015"
     CDS             1555..1953
                     /locus_tag="PLQ55_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HPK28934.1"
                     /translation="MTPVHDAATSYAINFLYETRNCDATGAVNNISSHKREELGNLFN
                     ELAPDVQADLKNATATLSGDLVSQAMARYVYILQKYGTVTFTNFIDRTLPPSGIPAVR
                     GDNKQNFYVLGIMAFIAVAGFFFLNRKRRY"
     gene            complement(2073..3047)
                     /locus_tag="PLQ55_03020"
     CDS             complement(2073..3047)
                     /locus_tag="PLQ55_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28935.1"
                     /translation="MKNAICRIGVLMAIAMGSLELVSMRIKEEPFDTSNTITRYTRET
                     SSTIRDSFFTTIDFKAAISDNTIIKGATLTADDSDMISLLNNDLYGIGYISYAALESS
                     ELKALIIEGVRVSEKSIIDDSYKLSRNFNYITKAPSDLSEKEYALITGFIAFSKSREG
                     LSIIKAKDGIVTQNIEEAESFTTLLAKSENSALAKMLYNTNEAPIHIKLGGSKSVESI
                     SRALSSAFTSLVPAFKAVHNYTSNEMAYEATQGSNKDSAEPLHIGFLARDLDINETEP
                     ALAGSYGVISKDAIVAVVSNKNHVLSDITRDQLKQIYKSETITWSALE"
BASE COUNT         1107 a          583 c          670 g         1127 t
ORIGIN      
        1 ttgtttttaa aaatttaatc tttatttgta aagataagta tgttatctaa ccgcataaag
       61 atatttgcta tcttgttaat ggtgaattaa aatagtaaag tgaaattgaa aatatttatt
      121 attttagtaa tattaatcac cgtgttcgca ttagtttgat aaaaaggggg aatcaaaaag
      181 atgaacaaaa atcgaaaatg gttagctagg agcgccgtta tttcgctatt aattagtgca
      241 attatcacac cagcgttttt tcgtaaggac gcggtaaaaa caaaagcggc aacgacgata
      301 ccggtcttag tcggcgattc aattggatgg gaaccatcag tgtcataaaa attcacggaa
      361 tatcccggcg gttctgatat ttatgtatat acctttacgc ggtcgcacgc tggatattac
      421 aaaatatatg actataatac tggggcacca atggaagacc ttcaatttga taccacacca
      481 tcgctttcac gcgattatat ttatcgatat gaagattcgc ccgatgctaa ctttattaca
      541 atttatcccg gagaaattaa ggttcaagtt tttaatcctg ataacgctgg gggtagtcct
      601 gcgggcgcaa ggcggatgaa agtcgagtat acaagtttac tcggggcaac accatcaaca
      661 ccaacaggta catcttcgta tgcattgcgg ggccctgctg ttggtgactg gacaacgcac
      721 gatattaata attgtttagt tggtatcagt ggttatgatg gccgttatct ttcacttact
      781 agagccttta acgctgatga ttttaaaatc tttgtgtaca aacaaacatt aaatgaacct
      841 tattaacgcg gatttattga ttttgatgat cataagtcga atgcaagtga tgtaattatg
      901 cctggtgatc cttcttcagg ttatcatgag agttttcatg taaatactgc tggaaattat
      961 atggtcgttt atgatcttaa aatgcagaaa atttactttc acaatgctgg tacaacgtat
     1021 cataaagtaa caaaatatta tggcgaactt ttaattgaag aagcagcagc ctttactggt
     1081 ttaaattata ccccggcacc attttatcaa ccagagaaaa aattcgttgg ttggtatgaa
     1141 gacgctttat tagaaacggc atatgttcca aaagatttaa caagtgacat tacattgtac
     1201 gctaaatttg tggattcacc gagtgttaat ttaaggtttt acgatcgtaa tttagatatt
     1261 aaagtggata acggcggatt taacgagtat gttgtatttg cttatggtaa gaatacagac
     1321 ggaactgaac cattaggagg ttggccaggc cggatgatgc aaaaagtagt agatatggga
     1381 catgaattat cttacggcta ttatgcactt aatatttcag gacaagatag accagaaaaa
     1441 gttactttta ataatggatg tcaacctttc gaaccagagc atgtttcaac cccggaactt
     1501 gtgttacccg ctgataagaa tctctatgaa cacgatgtag cagcgtagag tgtaatgact
     1561 ccagttcatg acgctgcaac aagttacgcc atcaattttc tctatgaaac aagaaactgt
     1621 gacgcaactg gggcggttaa taatatctca agtcataaac gggaggaact cggaaattta
     1681 tttaatgaat tagcgccaga tgtgcaagcg gacttaaaaa atgcgactgc aacattaagt
     1741 ggtgacttag tgtcacaagc tatggcgcgt tatgtatata ttttacaaaa atatggaacg
     1801 gttacattta caaacttcat tgaccgtaca ctaccaccaa gcggtatacc cgcagttcgt
     1861 ggtgataata aacaaaattt ctatgtacta ggcataatgg cctttatcgc tgtggcgggt
     1921 tttttcttcc taaatcgtaa gcgtcgttac taataagttt aatagttatt gtataaattt
     1981 aaaaatataa aaaagagcgg ttatcatcat tttatgaaga tgtaccgctc ctttaagaat
     2041 gttatcgtga cagttagata acaagtgatg cgctattcaa gtgcggacca cgtaattgtt
     2101 tcagatttgt aaatttgttt taattgatcg cgtgtgatgt cactaaggac atggttttta
     2161 ttgctgacga cagcaacgat agcgtcctta ctaattacgc cgtaactgcc agcaagtgcg
     2221 ggttcagttt cattaatatc taaatcccgg gctaaaaaac caatatgaag tggttcagcg
     2281 ctatctttat tactcccttg tgttgcttca taagccattt cattactagt gtagttatga
     2341 actgctttaa aggcgggcac taaagaagta aaagcactac ttaaggcacg actaatactt
     2401 tcgacagatt ttgaaccacc aagtttaatg tggattggtg cttcattagt gttataaagc
     2461 attttcgcta gagcgctatt ttcgcttttc gctaagagtg ttgtaaaact ttcagcctct
     2521 tcaatatttt gggtaacgat tccgtctttg gccttaataa tacttaagcc ttcgcgtgac
     2581 ttactaaagg cgatgaaccc tgtaattagg gcatattctt tttcacttaa gtcactagga
     2641 gcttttgtga tgtagttaaa gtttcgtgat aatttgtacg aatcatcaat aattgatttt
     2701 tcggagacgc gaaccccttc aattattaaa gctttaagct cggaagattc aagtgcggcg
     2761 taggaaatat atccaatacc gtataaatcg ttattgagta aagagatcat atcgctatca
     2821 tccgcagtta gtgtagctcc tttaataata gtgttatcac taatagcggc tttaaaatca
     2881 atcgtggtaa aaaaactgtc acgtatggtg gaagaagttt cccgtgtata acgggtaata
     2941 gtgttacttg tatcaaatgg ctcttcctta atccgcatgg atacaagctc gagactaccc
     3001 atagcaatcg ccataagtac tccgattctg caaattgcat ttttcatatc tgaacacctc
     3061 ctttcagaca cataaacttt aaagcaaacg gagagctaga tatatcataa agctgtaaaa
     3121 agttgtaaag aagttgtaaa agtggtagtt tttcgctgat gcttttggtt aaaatatcat
     3181 taaagtaata gttttattac taaaaaactt aaattttccc gcaaaatttt taattttaca
     3241 gttaatttag gtttgtttta agtgtgcctt tctcacaaaa aagtgacaaa ttaatagtta
     3301 aagatataaa aaacaataaa gcgaaatcta ttttttgatt cagttcatac tcctattgat
     3361 aaaccttcac ctgatcgttg cgcatgtata tttaatgttt actttgcaga tgatatgtta
     3421 attgttgatt ataagtaata agatacttgt acagttacga tactttattt attagctgca
     3481 tttttta
//
LOCUS       DAOPCD010000093         3461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_21871_length_3461_cov_5.156195, whole genome shotgun sequence.
ACCESSION   DAOPCD010000093 DAOPCD010000000
VERSION     DAOPCD010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21871_length_3461_cov_5.156195"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1171)
                     /locus_tag="PLQ55_03025"
     CDS             complement(29..1171)
                     /locus_tag="PLQ55_03025"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPK28936.1"
                     /translation="MENFFVIVSAFFYLSIMTASFIFLNRQDDQNSRRFRVVVLLYVF
                     TILGIILDTLYRLDYGGLINLPSYAFAGVITATYVCLPVMWGLWCLYISHTSYLKEIP
                     RAYNIVVAATLLLNLALSLISLIPGTNIYFTIVDGVYARGKLFFVQTIIFLLFLALTI
                     CFYTRFFKTFGKSERFTFLLIITTLILALVGHLLNNFIPFMVMNNIFNYMILAMNVQH
                     IHASTDFLTGLANRRSLSNHLHSRMTGTGKTSSFSIIMFDLDNFKMINDTLGHDAGDD
                     ALRQFARFLRHHCDNVDFVARFGGDEFIIVLDSMSPAELKKRVAVIREQFSEYEGNKV
                     GNFKLVFSAGYFIHNKDLKISTGELIRIIDERMFRDKRKRSNGKTN"
     regulatory      1489..1566
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1695..2534
                     /locus_tag="PLQ55_03030"
     CDS             1695..2534
                     /locus_tag="PLQ55_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28937.1"
                     /translation="MKNKKSIMFGALALVLLSGLSAASTTFAWFTTTRTASLTFSDAT
                     VVCSDSNLVVTYVDSLNTFGTISDEANEIKLTGANRVTDISGNGLDFYKPVWSPIEGV
                     ATNIKEVATAENYYIDFVLKIARQSDSPSATGIKVYLGENTKILPVDATKPRDVDAVS
                     AVRMAVISYADEFRASPALIELHAPVVETSPEYIIKDTASTAYSVNGYKVHDASAEVN
                     SDPFVTKLSEADGPTHQIADLSSDSEAYVGFRFWIEGTDAEATKDKALGGMFKVALDI
                     YSL"
     gene            2660..>3461
                     /locus_tag="PLQ55_03035"
     CDS             2660..>3461
                     /locus_tag="PLQ55_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28938.1"
                     /translation="MNKKNKVLLGAIGLVLLSGIAATSSTFAWFTTVRTASISYSEAT
                     VQSSDGNLAIEYLSSLNNWSGGSTYDAEANKVTIAGANKVTDISGDGKTFYKPVWTTP
                     VSTASSIETVPTTGSPGVADGFYIDFTVRLSRDNAVAENGLKVYLGTGTTIAPKDPEN
                     LADTGAVKAARLAVIDHEGDVKVRWAPEQETEPKYITTGTDSLYGVSGFETTTDLNYK
                     HGEITSAESKAAADLAGFLVADLSKGVATEANVTFRIWLEGEDSDCDND"
BASE COUNT         1187 a          658 c          663 g          953 t
ORIGIN      
        1 cgtacgcgct atgtgtgcgt aaaatttttt aattagtttt accattactg cgtttacgct
       61 tatctctaaa catccgctca tcgataatgc gaataagttc accagtgcta atttttaaat
      121 ctttgttatg gataaagtat cccgcgctga agactagttt aaaattaccg actttattac
      181 cttcatattc actaaattgt tcgcgaatga ccgcaacacg ttttttaagt tcagcgggcg
      241 acatactatc aagaacgata ataaattcgt caccgccaaa gcgtgcgaca aagtcaacat
      301 tgtcacaatg atggcgtaag aaccgcgcaa attgacgaag agcgtcgtcc ccggcatcgt
      361 gacccaatgt atcattaatc attttaaaat tatcaaggtc aaacataata atggaaaaac
      421 tgctcgtttt ccccgtacca gtcatgcgac tatgaaggtg gttacttaag ctccgacgat
      481 tagcaagacc agtcaagaaa tcagtagaag catgaatatg ttgaacattc attgccagaa
      541 tcatatagtt aaatatatta ttcataacca taaacggaat aaaattattt aataagtgac
      601 caacaagtgc taatatcaga gtagttataa taagtaagaa agtaaagcgc tcacttttcc
      661 caaatgtttt aaagaaacgc gtgtaaaaac aaatggtgag cgctaagaat aaaaggaaaa
      721 taattgtttg gacaaagaat aatttacctc tagcatatac gccatcaaca atagtaaagt
      781 aaatattagt gccaggaatt aaagatataa gtgaaagcgc taaatttaaa agtaaggtcg
      841 cagcaacaac aatgttataa gcgcgcggta tctcttttaa atatgaagtg tgactaatat
      901 ataagcacca taatccccac atgactggta agcaaacata tgtagcggta ataactccag
      961 caaaggcgta tgacggaaga ttaattagtc ccccataatc taaacgataa agtgtatcaa
     1021 gaattattcc taagattgta aacacataaa gtaagacgac aacccgaaag cgacgtgaat
     1081 tttgatcatc ttgccgattt aaaaatataa aagacgctgt catgattgac aggtagaaga
     1141 atgcgctaac aataacaaag aaattctcca tagatatatt ccagtgtgtt gtattatatc
     1201 tacatataat aatttttgaa aagttttaaa cttaaattta ctgatgtaag tgcttttatt
     1261 ttcgtcatag aatgcattaa atttaaaatt gttctcgaaa aatctaacaa aaatttcaat
     1321 tgtaaaattt tactttaaaa ataagaaaga taacgataac aaatgtaaat aaaatatata
     1381 aacaaccttt taaaccacca tagaatgata aaaataaatg gaaaaacgaa aaaaagttaa
     1441 aaaattttat attccttgtt gacaaaattt ctgaattata tataatcatg gtgtaaaagg
     1501 caaagctaga gcgatctagc gacgcaaaac tatagggtct tagcaataag atagccagct
     1561 accgagcgta agaataggtg gctgttttta ttactcggac ttaaaactag taatacatta
     1621 caaagaactt actcttccga ctcggtcaaa ccttgtaaga cacacgacaa gcgcaacgaa
     1681 aggaagatga acatatgaaa aacaaaaaat caattatgtt tggtgctcta gcgcttgttt
     1741 tacttagtgg tctttccgca gcaagtacaa cattcgcgtg gtttaccacg acacgtactg
     1801 cttcacttac ttttagtgat gcgactgttg tatgtagcga tagtaatctc gttgtgactt
     1861 acgttgattc actaaacact ttcggaacga ttagtgacga agcaaacgaa attaaattaa
     1921 cgggtgcgaa tcgtgtcaca gatatttccg gaaatggttt agatttttac aaacccgtgt
     1981 ggtcgccaat tgaaggcgtt gctacaaata ttaaagaagt agcgactgcc gaaaattact
     2041 acatcgactt cgtccttaag attgctagac aaagcgattc cccaagcgca acgggaatta
     2101 aagtctactt aggcgaaaat acgaagattc ttccggtcga tgccactaaa ccacgcgatg
     2161 ttgatgcagt aagcgcagta cgcatggcag taataagtta tgcggacgaa ttccgcgctt
     2221 cgcctgcact aattgaactg cacgcccctg tcgtcgaaac tagcccagaa tatattatta
     2281 aagatacggc tagcaccgcc tacagtgtta atggctataa agttcatgac gcaagtgcag
     2341 aagttaacag tgatccattc gttacaaaac ttagtgaagc ggatggaccc actcatcaaa
     2401 ttgccgattt atcgagtgac tccgaagcat acgttgggtt tcgcttctgg attgaaggta
     2461 cggacgcgga agcgaccaaa gacaaggcac tcggaggcat gttcaaagtt gccctagaca
     2521 tctatagtct ctaggcaagt actacactaa tatcaaagat attaaattag aacctaattg
     2581 cccaattagt tcatctcatc gatgaaagtg gggccggcca caacttccgg caattgttca
     2641 aaggaggata ttacatatta tgaacaaaaa gaataaagta ttattaggag cgatcggctt
     2701 agttctctta agtggaatcg ccgcgacaag ctcaaccttt gcatggttta caaccgtgcg
     2761 taccgctagc atctcttaca gcgaagctac agttcaatct tcagatggca acttagccat
     2821 tgaataccta agctcattaa ataattggag tggtggttca acttatgatg ctgaagccaa
     2881 taaagttaca attgctggtg ctaacaaagt tactgacatt tctggtgatg gtaaaacatt
     2941 ctataaacca gtatggacaa ccccagttag tactgcttca agcatcgaaa cagttccaac
     3001 cactggttct cctggtgttg ctgatggatt ctacattgac ttcaccgtcc gtttaagccg
     3061 tgataatgct gttgctgaaa acggtttaaa agtttacctt ggtactggta ccactattgc
     3121 gccaaaagat cctgaaaatc ttgcagatac tggtgcagtc aaagctgctc gtttagcagt
     3181 cattgatcat gaaggagacg ttaaagttcg ctgggctcca gaacaagaaa cagaaccaaa
     3241 atacattact actggtactg actcacttta cggtgtgtca ggtttcgaaa ctacaactga
     3301 tcttaactat aaacacggag aaatcacttc tgcagagagc aaggccgctg ctgatctggc
     3361 tggtttctta gttgctgact taagtaaagg tgttgctacc gaagcaaacg ttaccttccg
     3421 tatttggtta gaaggtgaag atagcgactg tgataacgac a
//
LOCUS       DAOPCD010000094         3439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_22043_length_3439_cov_3.596040, whole genome shotgun sequence.
ACCESSION   DAOPCD010000094 DAOPCD010000000
VERSION     DAOPCD010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22043_length_3439_cov_3.596040"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1862)
                     /locus_tag="PLQ55_03040"
     CDS             complement(<1..1862)
                     /locus_tag="PLQ55_03040"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HPK28939.1"
                     /translation="MKNKKLVSDIAIDGLFIALILVLSLVPYLGFIQIGGISATILPI
                     PVILGAALLGPRRGVLYGAAFGFSSFLIAVIRGTAGDALFVDPLISIVPRILFGFCTA
                     IFSAVSFNERTSFKLKRFLIFPYSAIMMLLHSFFVLLAMYLRYVNAFMEYIFPILTPL
                     VLLEALVATVIVPVLYNVLYIPFEKYKDKFTTKNKSIYGTITSVYFADALNSLKEFVS
                     IKSVYDEKTVTKKTPYGKGVNEALEYMKNLATNDGFEAKIIDGRVVEIFVGEKYNKNI
                     AVFAHADVVPATGEWDTPPFTADIREGKLYGRGTSDDKGPAIAAYYAIKTLNNNNLLI
                     NYSVRLVIGGDEERGSSCMHYYFNEYNAPAPVHGFTPDAEFPLIYGEKGITNFTATKM
                     IDLGPISTITGGEAANSVIDKVVIRLLKDEDFIKYLTDNKVEHTVKMLSKNMDVTIFG
                     KSAHGSLPELGVNAGVLAFKHLGAFYKLPFLTHLAEKFKNPNGKTMDAYVATSLLGAT
                     TYNIGLLNYENGKLSFVVNFRYPENVEVETHLAKLAQTIDVELEIGRSSKHLLFDPKS
                     EFIQTLLKAYRDETGDTQSKPLAIGGGTYAKECPNTVAFGSAFPSRSGDIHSAN"
     gene            complement(1852..2634)
                     /locus_tag="PLQ55_03045"
     CDS             complement(1852..2634)
                     /locus_tag="PLQ55_03045"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPK28940.1"
                     /translation="MYLTVDIGNSVISIALFNAGEIVDVFNRETPKALKETAQANFDT
                     VRPIINEFKKQYAHSTFKKVLISSVVPKLNYPMSFVIKEILGVDAEIVLPNPKTSVRL
                     DVDVPESVGSDIVACAVGAYKFGKGPFIVIDLGTANKYFYVDDKHVFHGVAISPGVEV
                     SFKGLVKNAAQLFNVRLTLPAKVLGKNTPDSLNSGLLYGLLGEMNGFINLIKKEVGDN
                     PIVLLTGGNARYIKGAVDESVNYVPHLVHYGLYDMLVNGNEK"
     gene            complement(2692..2865)
                     /locus_tag="PLQ55_03050"
     CDS             complement(2692..2865)
                     /locus_tag="PLQ55_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28941.1"
                     /translation="MQVNGKLRATIKAKLNESKEELEKAALTNENVQRFIDGLTIRRV
                     IVVPNKIVNIVAS"
     gene            complement(2869..2985)
                     /locus_tag="PLQ55_03055"
                     /pseudo
     CDS             complement(2869..2985)
                     /locus_tag="PLQ55_03055"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3003..>3439)
                     /locus_tag="PLQ55_03060"
     CDS             complement(3003..>3439)
                     /locus_tag="PLQ55_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPK28942.1"
                     /translation="VNTKEPFTKLFHQGMILGENGEKMSKSRGNVVKPDDIIAEYSVD
                     ALRLYEMFMGPLEASLPWSNTGLEGALRFLNRVARINSEDFPGKLVDTNNGELDYSYH
                     YTVKKVTEDYEKLAFNTAIAQMMILVNDMYKAREVLFLITSV"
BASE COUNT         1135 a          717 c          512 g         1075 t
ORIGIN      
        1 tcgttagcgg aatgaatatc cccgcttcta ctaggaaaag cactaccaaa tgcaacagta
       61 ttaggacatt ctttagcata agttccgccc ccgatagcaa gtggcttact ttgcgtgtcg
      121 cctgtttcat cgcgatatgc ttttaagagt gtttgaataa attcactctt tggatcaaat
      181 aataaatgtt tactacttct accaatttct aattcgacat cgattgtttg cgctaatttt
      241 gctaaatgcg tttccacttc cacattttcc ggataacgga aattaacaac aaaacttaat
      301 ttcccgtttt cataatttag taatccgata ttatatgtcg tagcaccaag cagtgaggtt
      361 gcgacatatg catccatagt tttaccgttt ggatttttaa atttttccgc taaatgggta
      421 agaaatggaa gtttatagaa agcacctaaa tgtttaaaag caagtacccc ggcgttcaca
      481 ccaagttcag gtaaactacc atgcgcactt ttaccaaaaa ttgttacatc catatttttc
      541 gataacattt ttactgtatg ctcaacctta ttatccgtta aatatttaat aaaatcttca
      601 tcttttaaaa gccgaatgac gactttgtca atcacactat tagcagcttc accaccagta
      661 atcgtgctta ttggacctaa atcaatcatc tttgtcgcgg tgaaattagt aatacctttt
      721 tcaccatata ttagcggaaa ctccgcatca ggtgtaaaac cgtgtaccgg tgcaggcgca
      781 ttatattcat taaagtaata gtgcatacaa ctgctacccc gttcttcgtc gccaccaatt
      841 acaagacgga cactataatt aattaataag ttattattat tcaaagtttt aattgcgtaa
      901 taagcagcaa tcgctggtcc tttgtcatca cttgtgccac ggccgtagag ttttccttca
      961 cgaatatcag cggtaaatgg tggtgtatcc cattcgccag ttgctggcac gacatcagcg
     1021 tgagcaaaaa cagcaatatt tttgttatac ttttcaccaa cgaatatttc aacgacacgg
     1081 ccatcaataa ttttcgcttc aaatccgtca tttgtggcaa gatttttcat atactctaat
     1141 gcttcgttaa cccctttacc atatggtgtc tttttcgtaa ctgttttttc gtcatatacc
     1201 gatttaatac tcacaaattc ttttaaagaa tttaaagcat cggcaaaata aacactggta
     1261 atcgttccat aaattgattt atttttcgta gtgaatttat ccttatattt ttcaaaagga
     1321 atgtataaaa cattgtataa aacaggaaca ataacagttg ccactaaagc ttcaagtaat
     1381 acaagcggag taagaatcgg gaaaatatat tccataaaag catttacata acgcaaatac
     1441 attgcaagta agacaaaaaa tgaatgtaac agcatcataa ttgctgaata agggaatatc
     1501 aggaaacgtt ttaatttaaa actagtgcgt tcattaaatg aaaccgctga aaatattgcg
     1561 gtacaaaatc caaataatat ccgcggcaca atactaatta aaggatcaac aaataatgcg
     1621 tccccagcag taccacgaat aacagcaatt aaaaaggacg aaaaaccaaa agcagcgcca
     1681 taaagcacac cgcgtctagg tcctaaaagt gccgctccta aaattacagg aattggtaag
     1741 attgtcgccg aaatccctcc gatttgaata aagcctaaat acgggacaag acttaacact
     1801 aaaataaggg caataaataa cccatcaatt gcaatatcac ttactaactt tttatttttc
     1861 attgccattt accaacatgt catataaccc gtaatgcact aaatgtggaa cataatttac
     1921 actttcatcc acagcacctt taatatagcg tgcattacca ccagttaata acacaattgg
     1981 attatctcct acttcttttt ttattaaatt aataaacccg ttcatctccc ctaataaacc
     2041 atataataaa ccgctattaa gcgagtcggg tgtattttta ccgagtactt tcgcgggtaa
     2101 agtaaggcgg acattaaata attgtgctgc attttttact aaacctttaa acgatacttc
     2161 cacaccagga ctaattgcca ctccgtgaaa aacatgttta tcatcgacat aaaaatattt
     2221 attagccgtc cctaaatcaa taacaataaa aggacctttc ccaaatttat aagcaccaac
     2281 agcgcaagcc acaatatcac taccaacgct ttcgggaaca tcaacatcta gtctcacact
     2341 agttttagga tttggtaaaa caatctcagc atcgactcct aaaatttctt taatcacaaa
     2401 tgacattgga taatttaact tcgggacaac actagaaata agcactttct taaatgtact
     2461 gtgcgcatat tgctttttaa attcgttaat gatgggacga acggtatcaa agtttgcttg
     2521 tgccgtttct tttagcgctt tcggcgtttc acgattaaaa acatcaacaa tttcaccagc
     2581 gttaaatagc gcaatactaa ttacactatt tccgatatca actgttaaat acataagctc
     2641 tccccaccac ttttactaaa taagtgcgta atatattata atatataatt cctaagaagc
     2701 aacgatattt acgattttat ttgggacgac aatgacccgt ctaatcgtta aaccatcaat
     2761 aaaacgctgg acattttcat tagtgagcgc ggccttttca agttcttctt tactttcatt
     2821 aagtttagct ttaattgtcg cgcgtaattt accattaact tgcacagcaa tttcaacttc
     2881 atttacgact aactttgctt catcgtaagt tggccacgct tcataagtta ttgtgtcaac
     2941 atcaacgaat tgtgagtaaa gttcttcgcc taaatgtgga gcgattggat aaagtaattt
     3001 aattaaaccg acgtaataag gaataaaact tcacgcgctt tatacatatc attaactaaa
     3061 atcatcatct gcgcaatcgc ggtattaaac gctaattttt cgtagtcttc agtcactttt
     3121 ttaacagtgt agtgatacga gtaatcaagc tcaccattat tcgtatccac aagttttcca
     3181 gggaaatctt cactattaat tcgtgcgaca cgatttaaga aacggagcgc accttctaac
     3241 cctgtatttg accacggtaa gcttgcttca agtggaccca taaacatttc ataaagccgc
     3301 aaagcatcaa cactatattc agcaataata tcatcaggtt taacgacatt accacgtgac
     3361 ttcgacattt tttcaccatt ttcccctaaa atcatccctt gatggaataa tttagtaaat
     3421 ggttcttttg tatttacaa
//
LOCUS       DAOPCD010000095         3439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_22044_length_3439_cov_3.443262, whole genome shotgun sequence.
ACCESSION   DAOPCD010000095 DAOPCD010000000
VERSION     DAOPCD010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22044_length_3439_cov_3.443262"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PLQ55_03065"
     CDS             <1..665
                     /locus_tag="PLQ55_03065"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-diphosphate and nicotinate from nicotinate
                     D-ribonucleotide and diphosphate; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HPK28943.1"
                     /translation="SMADRQDDYLTQAGDGYASYIAGIKKFSTDAQGAWVNVKGMGTM
                     PHALIAMTNGDLIKACELYHKTFPNEKISALVDYHNDTVTEALKVARHFGAKLGSIRL
                     DNSKALLDEYFVRQGLNDPSLKGVSPELVFAVREALDKEGFNYVNIVVSSGFTPEKIK
                     YFKAKNAPVNLYGVGSFIVNNKEVGFTGDVVVVNGKPQAKVGRQEYFSDKLEKVTLKK
                     RM"
     gene            739..1743
                     /locus_tag="PLQ55_03070"
     CDS             739..1743
                     /locus_tag="PLQ55_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK28944.1"
                     /translation="MSDAYKDRVTIYEVAAAAGVSLATVSRVINNHPNVTPATRAKVQ
                     NAITRLGYRPSALAQALATSKTTNIGLIIPSANYVFISNMLHGIVMRANSLGYLITLF
                     VTGTEKEDALDAMDKLISSHVDGAIIFDDLFEEEEVTQITNYQVPVVVVNNRLDEDEK
                     VACITFGYENTLREDIIRHHLKTSDVMMHFVALNDNGRLLDRLQRSFVNTHKEENREY
                     KIHKVQDGYHAHYAYFKELFKTTKEGFFVAYRDSLGRAIVNAALENGLRIPEDIEVIS
                     LIGTRYAVQGRPKLSSMILDMEEVGNKALEMLIEILEGKTHLPRQKVVAEYIKLDTTR
                     "
     gene            1755..3005
                     /gene="tyrS"
                     /locus_tag="PLQ55_03075"
     CDS             1755..3005
                     /gene="tyrS"
                     /locus_tag="PLQ55_03075"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPK28945.1"
                     /translation="MNIFDELEYRGLIKDYSNAANIKEMLNTPQKIYCGFDPSASSMH
                     MGNFVMIMTLMRLQRAGHKIIAVVGGATGMIGDPSGKSAERNLLDKDALRHNTAQIRA
                     QLEKYLDLSDPQKGEIVNNYDWHGNINVLEFLRDYGKYFTVNYMLAKDTVARRLETGI
                     SYAEFSYMLLQAVDFLYLYEHYACTMQIGGSDQWGNLVSGLDLIRKVKGADAKVEVMT
                     AHLLTDAEGNKFGKSVDGALFLDAKKFSPYKLYQYFYNVGDADAEKYLKVFTFLSREE
                     IEAITSEHKEAPHRRLAQTRLAEEIVTIVHSKEALLHAQNMSTALFSGNVKDLSAEDI
                     EDLFAHALVGLDSDQKLEDVLITLKAATSKREAREFIRGNSISVNGERLTDPELIITK
                     DYLLHDKYLIIRRGKKNYFVAEVK"
     gene            complement(3072..>3439)
                     /gene="rpsD"
                     /locus_tag="PLQ55_03080"
     CDS             complement(3072..>3439)
                     /gene="rpsD"
                     /locus_tag="PLQ55_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012010904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPK28946.1"
                     /translation="TGLAFIQILESRLDNLVFRLGLAATRNQARQLVNHGHIEVDGQK
                     VDIPSFLVPVGSTITVRENSRDLKLFKFNLEKVGRTVPFVTLNAEKLSGTFDRMPERT
                     EVAQDIQENLIIEFYNRSM"
BASE COUNT         1109 a          569 c          743 g         1018 t
ORIGIN      
        1 ttagtatggc agaccgccaa gatgattatt taacacaggc gggagatggc tacgctagtt
       61 atattgcggg gattaaaaag ttttccactg atgcgcaagg tgcgtgggta aatgttaaag
      121 ggatgggaac aatgccacat gctttaattg cgatgactaa tggtgactta attaaggctt
      181 gtgaacttta tcacaaaact ttcccaaatg aaaagatcag tgcgcttgtt gactatcata
      241 atgatacagt gacagaggcc ttaaaagtag cgcgtcattt tggtgcgaaa ttaggctcga
      301 ttcgtcttga taatagtaaa gcattacttg atgaatattt tgtgcgtcaa ggtttaaatg
      361 atccaagtct aaaaggtgtt agtcctgaac tagtcttcgc cgtgcgtgaa gcacttgata
      421 aagaaggttt taattatgta aatatcgtcg taagttctgg ctttactcct gaaaaaatca
      481 aatattttaa agccaaaaat gctcctgtta atctctatgg tgtaggctct tttattgtga
      541 acaataaaga agtcggattt actggtgatg ttgtcgttgt taacggcaaa ccgcaggcaa
      601 aagttggacg gcaagaatac ttcagtgata agcttgaaaa agtaacactt aaaaagcgaa
      661 tgtaagggct ttctcttcta cattattgac aatgaaaata ttttcaagta aaattggtat
      721 gaaagaaggc tttttaatat gagtgatgca tataaagaca gagtaacaat ctacgaagta
      781 gcagcagcgg ctggcgttag cctagcaact gtgtcgcgtg tcattaataa tcatccaaat
      841 gtcacaccag cgacgcgggc taaagtccaa aacgccatta cgcgccttgg ttatcgtcca
      901 agtgctttag cgcaagcgct agccacgagt aaaacgacga atattggctt aattattcct
      961 agtgccaact atgtatttat ttcaaatatg ctccacggca tcgtgatgcg tgcgaatagt
     1021 ttagggtatc taattacttt atttgtcact ggtacagaaa aagaagatgc acttgacgct
     1081 atggataaat taattagttc gcatgttgat ggggcaatta tctttgatga cttatttgaa
     1141 gaggaagaag taacgcaaat tacgaattat caagttcctg ttgttgtcgt taataatcgt
     1201 cttgatgaag atgaaaaagt tgcatgtatt acttttggtt atgaaaatac attacgtgaa
     1261 gatattattc gtcatcacct aaaaacaagt gatgtgatga tgcattttgt agcacttaat
     1321 gataatggtc gtttgttaga tcgtttacaa agaagctttg ttaacacaca taaagaagaa
     1381 aatcgtgaat ataagattca caaagtacaa gacggatatc acgcgcatta tgcttacttt
     1441 aaagaactat ttaagacgac aaaagaaggg ttctttgttg cttatcgtga ctcattagga
     1501 cgcgcgattg ttaatgccgc gcttgaaaat ggcttacgca ttccagaaga cattgaagta
     1561 attagtttaa ttgggacacg ctatgctgtg caaggccgcc caaaattatc aagtatgatt
     1621 cttgatatgg aagaagttgg taataaagca ttagaaatgc taattgaaat attagagggt
     1681 aagacacact taccgcgaca aaaagttgta gccgaataca taaaacttga tacaactcgc
     1741 tagaaaggtt cgttatgaat atatttgatg aattagagta tcgtggttta attaaagatt
     1801 actctaacgc tgctaacatt aaagaaatgt taaatacgcc acaaaaaatc tattgtgggt
     1861 ttgacccaag tgcttcaagt atgcatatgg gaaattttgt gatgattatg actctgatgc
     1921 gcttacaaag agcagggcat aaaattattg ctgttgtcgg tggtgcgacg gggatgattg
     1981 gtgacccaag cggtaaaagc gctgaacgta atttacttga taaagatgct ttacgacata
     2041 ataccgcgca gattcgcgct caattagaga aatatctaga tttaagtgac ccccaaaaag
     2101 gtgaaattgt taataattat gactggcatg gtaacattaa tgttttagaa tttttacgcg
     2161 attacggcaa atattttacc gttaactata tgttagcaaa agacactgtc gcgcgtcgtc
     2221 ttgaaactgg aattagctat gctgaattta gttatatgct cttacaagcc gttgactttt
     2281 tatatctcta tgaacattac gcttgcacta tgcaaattgg gggtagtgac cagtggggca
     2341 acttagttag cggacttgac cttattcgta aggtaaaagg tgctgatgca aaagttgaag
     2401 taatgacggc acacttatta acagatgcgg aaggtaataa atttggtaag agtgtggacg
     2461 gcgcactttt ccttgacgca aagaaattta gtccatataa gctttatcaa tacttttata
     2521 atgtcggtga cgctgatgct gaaaagtatc ttaaagtatt taccttttta agtagggaag
     2581 aaattgaagc aattactagt gaacataaag aagcaccaca ccgccggctt gcccaaacta
     2641 gactcgccga agaaattgtg acaattgttc atagtaaaga agccttactc catgcccaaa
     2701 atatgagcac tgcccttttt agcgggaatg ttaaagattt aagtgccgaa gacattgaag
     2761 atttattcgc ccatgcacta gtggggcttg acagtgacca aaaactcgaa gatgttctta
     2821 ttacgctaaa agcggcgaca agtaaacgtg aagctcgtga atttattcgt ggtaacagca
     2881 tcagtgtaaa tggcgaacgt cttactgatc cagaattaat tattactaaa gattatttat
     2941 tacacgataa atatttaatt atccgccgtg gtaagaagaa ctactttgta gccgaagtaa
     3001 agtaaaaagc tgcaaattga aaatcaaaaa aactccgcta ttatgcggag tttttgatta
     3061 agtctgctta attacattga acgattgtag aattcaatga ttaagttttc ttggatgtct
     3121 tgagcaactt cagtgcgttc tggcatccgg tcaaatgtac cgcttaactt ttcagcgtta
     3181 agcgtcacaa atggaactgt acgaccaact ttttcaagat tgaacttaaa gagttttaag
     3241 tcgcgactat tttcacggac cgtaatggtg ctgccaactg ggactaagaa gcttgggata
     3301 tcaacttttt gaccgtcgac ttcaatatgg ccgtggttaa cgagttgacg agcttggttg
     3361 cgggtcgctg cgagtccaag acggaacact aagttatcaa ggcgtgattc aagaatttga
     3421 ataaacgcta aaccggtaa
//
LOCUS       DAOPCD010000096         3381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_22476_length_3381_cov_4.365304, whole genome shotgun sequence.
ACCESSION   DAOPCD010000096 DAOPCD010000000
VERSION     DAOPCD010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22476_length_3381_cov_4.365304"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PLQ55_03085"
     CDS             <1..600
                     /locus_tag="PLQ55_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_148258436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HPK28947.1"
                     /translation="NEAGIKSVTLIIEGNNAYGHLRSEKGVHRLVRISPFDANKRRHT
                     SFASVNVVPIFDETGDIEINDSDLRVDTYRSGGAGGQNVNKVETAVRITHVPTGIVVS
                     CQMERSQLMNREIAMRMLKSELYQLELERQKAKVSAIEGEKKMIEWGSQIRSYTFAPY
                     TLIKDHRTNYEVGDIARVMDGDIAEFMYKYLEMEARSNG"
     gene            593..1564
                     /locus_tag="PLQ55_03090"
     CDS             593..1564
                     /locus_tag="PLQ55_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPK28948.1"
                     /translation="MAKQRIKDIFKEDWRTLLKNYSLIVVGSILLAFGTGVFLLPATL
                     NTGGLSGVGIIGEGLFGFDADLVVLVMTWAFFFISLLFLGWRFTIKSLVSSFVYPLAL
                     ILLMRLPAIATETVKLFGEGADVATKLIAGVFGGVFNGVGVALTFRGGGSTGGVDILV
                     VILNKYLRLSYSVMSFVIDGLIIAVGLFVLKDVVLSLIGILSAFVFAITLEFVFVGKK
                     HAMTASIISREKSAEINDFIQNEIGQGTTLVPVLGGYQKDEYMMVVVHFDRSNYARLI
                     NGIAKIDKKAFVSIEGSTVVLGGNFESLNPVKEGLVNIKKKDEKDKA"
     gene            1545..>3381
                     /gene="uvrB"
                     /locus_tag="PLQ55_03095"
     CDS             1545..>3381
                     /gene="uvrB"
                     /locus_tag="PLQ55_03095"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPK28949.1"
                     /translation="MKKTKHKFVLETTFTPTGDQPTAIKELVAGLERGDKHQVLLGAT
                     GTGKTFTIANVIEKVQKPTLILVHNKTLAGQLYAEFKALFPNNRVEYFVSNFDFYQPE
                     AYVPSRDMYIEKEAVQNAEIEMLRSAAINSLLERNDTIIVASVAAIYGLSDPEEYSKL
                     SFNIVEGEVINRKEFLIKLVDAQYTRNDYELRAGTFRVRGDVIEIMLPISEDYLIQVD
                     TFDDTIEKIREIDVLTGEVRKTFNTYPIFPAYAYASSRERINAIVPKILSDLASRLSY
                     FADEGKLLEAERLEFRTRQDVDSLQEFGICPGIENYARYIDGRKAGEKPYTLMDYFPK
                     DYLLVIDESHVTLPQVRGMFNGDRSRKQNLVDYGFRLPSALDNRPLQFNEFEEMINQV
                     IYTSATPGDYELDKTGGVVVEQLIRPTGLVDPEVFIKPSVGQIDDLMYELTKVIERGE
                     RAMVVTLTIKMAEDLTHYLKEHDFKVVYLHNETKTLERSQIIYELRKGKYDVLVGINL
                     LREGLDIPEVSLVAIIDADKEGFLRSERSLIQVMGRASRNVNGKVIMYANNMTGSMER
                     AIAETKRRRDLQIAYNKKHGIVPTTIIKPLGEPLHNFGTERELERI"
BASE COUNT         1076 a          496 c          758 g         1051 t
ORIGIN      
        1 aacgaagccg gcataaaaag tgtaacactt attattgaag gtaataatgc ttatggtcat
       61 ttgcgtagtg aaaaaggagt acaccgctta gtgcgtatct caccttttga cgcgaataaa
      121 agaagacata cttcatttgc gagtgttaat gttgtgccaa tttttgatga aactggtgat
      181 attgaaatta atgatagtga tttaagggtt gacacttatc gcagtggtgg tgctggcggt
      241 caaaatgtta acaaagttga gactgcagtg cgtattactc atgtcccgac gggtatcgtt
      301 gtatcgtgtc aaatggaacg cagtcaatta atgaatcgtg aaattgcgat gcggatgctt
      361 aaaagtgaac tatatcaact agaattagaa cgacaaaaag caaaggtaag cgcgattgaa
      421 ggtgaaaaga agatgattga atggggtagc caaataagat cttatacttt tgcaccctat
      481 acccttatta aagatcaccg cacaaattat gaagtcggtg atattgcacg ggtgatggac
      541 ggtgacatag cggaatttat gtataaatac cttgaaatgg aggcaagaag caatggctaa
      601 acaaagaata aaagatatat ttaaagagga ttggagaact ttacttaaaa actatagttt
      661 aattgttgtt ggatcaattt tactagcgtt tggtactggt gtttttcttt tacccgcaac
      721 acttaatact ggggggttaa gtggtgtcgg cattattggt gaaggcttat ttggttttga
      781 cgctgattta gtcgtacttg tgatgacttg ggctttcttc tttattagtt tattattctt
      841 aggttggcgc tttacaatta aatcgcttgt tagttcattt gtttatcctt tagcgcttat
      901 tttactcatg cgtttaccag caattgcaac tgaaaccgtt aaattgtttg gtgagggcgc
      961 tgatgttgcc actaaactta ttgccggtgt ctttggtggt gtgtttaatg gtgttggtgt
     1021 ggcattaact tttagaggtg gcggttcaac gggcggagtg gatattttag tagtaatttt
     1081 aaataaatat ttaagactaa gttatagtgt tatgtctttt gtgattgatg gcttaattat
     1141 tgccgttggg ttattcgtgc taaaagatgt tgtactctct ttaatcggta ttcttagtgc
     1201 atttgtgttt gcgattactc ttgaatttgt cttcgttggt aaaaagcatg cgatgactgc
     1261 ttcgattata agtcgtgaaa aaagtgcaga aattaatgat tttattcaaa acgaaattgg
     1321 tcaaggtaca accttagtgc cagttttagg tggttatcaa aaagatgagt acatgatggt
     1381 tgttgtccat tttgatcgta gtaattacgc tcgtttaata aacggtattg ctaaaatcga
     1441 taaaaaagct tttgtgagta ttgaagggtc aacagtagtg ttaggcggta attttgaaag
     1501 tttaaatcca gttaaagaag gcttagtaaa tattaaaaag aaagatgaaa aagacaaagc
     1561 ataaatttgt tttagaaacg acttttacgc ccactggtga tcaaccaacg gcgattaaag
     1621 agttagtagc tgggctagag cgcggtgata agcatcaagt acttttagga gcaacaggca
     1681 ctggtaaaac ttttaccatc gctaatgtta ttgaaaaagt gcaaaaacca acgctgatct
     1741 tagtgcataa taaaacacta gcggggcaac tttacgctga atttaaagca ttgtttccta
     1801 ataatagagt ggaatatttt gtgtctaact ttgattttta tcaaccagaa gcttatgtgc
     1861 catcacgcga tatgtatatt gaaaaagaag cagtgcaaaa tgccgaaatt gaaatgcttc
     1921 gctctgctgc tattaatagt ttactcgagc gtaatgacac aattattgtc gctagtgtcg
     1981 cggcaattta tggattaagt gaccctgaag aatatagtaa actatccttt aatattgtgg
     2041 aaggtgaagt gattaatcgc aaagagtttt taattaaact cgtggatgcg caatacacac
     2101 gtaatgatta cgaacttcgc gctggtactt ttagagtacg gggtgacgta attgaaataa
     2161 tgctccctat cagtgaagac tatttaattc aagttgatac atttgacgac actattgaaa
     2221 agattagaga aatagatgtt ttaactggtg aagtgcgcaa aacatttaac acttatccaa
     2281 tttttcccgc atatgcctat gcttcaagtc gtgaacggat aaatgccatt gtgccaaaaa
     2341 tattaagtga cttagcaagt agattaagtt actttgccga tgaaggtaaa ctccttgaag
     2401 ccgaacggct ggaatttaga acaagacaag atgttgattc actccaagaa tttgggattt
     2461 gtcctgggat tgaaaattat gcgcgttata ttgatgggcg taaagctgga gaaaagcctt
     2521 ataccttaat ggattatttt cctaaagatt atttacttgt gattgatgag agtcatgtta
     2581 ctttacctca ggtaagaggg atgtttaatg gtgatcgcag tcgtaaacaa aatctagtgg
     2641 actacggttt tagattaccg agtgctttag ataatcgccc gctacaattt aatgaatttg
     2701 aagagatgat taatcaagta atttacacaa gcgcaacacc tggtgattat gagttagata
     2761 aaacaggtgg agtagtcgtt gaacaactaa tccggccaac aggtttagtg gatccagaag
     2821 tatttattaa accgagtgtt ggacaaattg atgatttaat gtatgaatta acgaaagtta
     2881 ttgaacgtgg tgaaagagca atggttgtca cgcttacaat taaaatggcg gaagacctaa
     2941 cccattactt aaaagagcat gactttaaag ttgtctatct ccataatgaa acaaagacac
     3001 tggaacgcag tcaaataatt tatgaattac gcaaagggaa atatgatgtg cttgtcggga
     3061 ttaatttact gcgtgaagga ctagatattc ctgaagtttc acttgtggcg attattgacg
     3121 cggataaaga gggattttta cgtagtgagc gatcacttat tcaagtaatg ggccgcgcga
     3181 gtcgaaatgt taacgggaaa gtaataatgt atgcgaacaa tatgacgggg tcaatggaac
     3241 gcgctattgc ggaaacaaaa agaagaagag atttacaaat tgcttataat aaaaagcacg
     3301 gaattgttcc caccacaatt atcaaaccac ttggcgaacc actccataat ttcgggacag
     3361 agcgtgaact tgagcggatt a
//
LOCUS       DAOPCD010000097         3380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_22485_length_3380_cov_5.358195, whole genome shotgun sequence.
ACCESSION   DAOPCD010000097 DAOPCD010000000
VERSION     DAOPCD010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22485_length_3380_cov_5.358195"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..668)
                     /locus_tag="PLQ55_03100"
     CDS             complement(63..668)
                     /locus_tag="PLQ55_03100"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPK28950.1"
                     /translation="MNLSHYFTNNNELEHDIRTLELSLQGTNLTLITDRGVFSNKEID
                     EGSIVLLKSIIKSIDVKGEVLDVGCGYGTLGLYLAARYPEIKVTMFDINERAVSLTSE
                     NAKRNNLTNTHVYVDSDYQALPKHTFNLIVINPPIHAGKKAYYPLLSAARDYLTDGGI
                     LMFVIRKSHGAKSAAKYLSDYYSNITLIQKDAGFYVYHAQK"
     gene            complement(733..1107)
                     /gene="rplL"
                     /locus_tag="PLQ55_03105"
     CDS             complement(733..1107)
                     /gene="rplL"
                     /locus_tag="PLQ55_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPK28951.1"
                     /translation="MAKLTVTDIIAALKEMTVLELNDLVKAVEEEFGVTAAVATAGPA
                     PEDDGPVDKGPSEVTLVLKHWGQSKVAVIKAVQAITGLGLMDAKKLVDGAPVNIKEKI
                     SPVEAEEHKKALVEAGADVEIK"
     gene            complement(1132..1620)
                     /gene="rplJ"
                     /locus_tag="PLQ55_03110"
     CDS             complement(1132..1620)
                     /gene="rplJ"
                     /locus_tag="PLQ55_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPK28952.1"
                     /translation="MNQKSIVAKEAIVKEVVDLAKSHEAFIVFTYHGLSVSQITELRR
                     LLRADGNTMGVYKNSLVERAVKELGREELNDYLTGPNAFLFSKDVSSGAKALTRFARF
                     NDVVEIKGGLFEGNRVVDGAEVRNIGRLPDRNGMISMFLSVLNAPIQKFAATVKAVAE
                     KQ"
     gene            1761..2249
                     /gene="rplJ"
                     /locus_tag="PLQ55_03115"
     CDS             1761..2249
                     /gene="rplJ"
                     /locus_tag="PLQ55_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPK28953.1"
                     /translation="MNQKSIVAKEAIVKEVVDLAKSHEAFIVFTYHGLSVSQITELRR
                     LLRADGNTMGVYKNSLVERAVKELGREELNDYLTGPNAFLFSKDVSSGAKALTRFARF
                     NDVVEIKGGLFEGNRVVDGAEVRNIGRLPDRNGMISMFLSVLNAPIQKFAATVKAVAE
                     KQ"
     gene            2274..2648
                     /gene="rplL"
                     /locus_tag="PLQ55_03120"
     CDS             2274..2648
                     /gene="rplL"
                     /locus_tag="PLQ55_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPK28954.1"
                     /translation="MAKLTVTDIIAALKEMTVLELNDLVKAVEEEFGVTAAVATAGPA
                     PEDDGPVDKGPSEVTLVLKHWGQSKVAVIKAVQAITGLGLMDAKKLVDGAPVNIKEKI
                     SPVEAEEHKKALVEAGADVEIK"
     gene            2713..3318
                     /locus_tag="PLQ55_03125"
     CDS             2713..3318
                     /locus_tag="PLQ55_03125"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPK28955.1"
                     /translation="MNLSHYFTNNNELEHDIRTLELSLQGTNLTLITDRGVFSNKEID
                     EGSIVLLKSIIKSIDVKGEVLDVGCGYGTLGLYLAARYPEIKVTMFDINERAVSLTSE
                     NAKRNNLTNTHVYVDSDYQALPKHTFNLIVINPPIHAGKKAYYPLLSAARDYLTDGGI
                     LMFVIRKSHGAKSAAKYLSDYYSNITLIQKDAGFYVYHAQK"
BASE COUNT         1026 a          664 c          664 g         1026 t
ORIGIN      
        1 tggagtcttt ttgttttatc gttaattgtt ttcataaatg gcctcgcttt acttgtattt
       61 tgttactttt gtgcgtgata gacgtagaat cccgcatctt tttgtataag tgtgatattt
      121 gaataatagt cacttaagta ctttgcggca cttttagcgc catgggactt acgaatcaca
      181 aacatcagta ttccgccatc agtgagatag tcacgcgccg cactaagaag ggggtaataa
      241 gcttttttac ccgcatgaat tggtgggtta ataactatca agttgaatgt atgtttaggt
      301 agagcctgat agtcactatc tacataaaca tgtgtatttg tgaggttgtt gcgtttagcg
      361 ttctcactcg ttagacttac agcgcgttca ttaatgtcga acattgtgac tttaatttcg
      421 gggtagcgtg cggctaaata taagccaagc gtcccgtaac cacacccgac atcaagtact
      481 tcgcccttta cgtctatgct cttaataata cttttgagta gtacaatgga cccttcatca
      541 atttctttat tgctgaagac tccgcgatca gtaattagcg ttaaatttgt gccttgtaat
      601 gataattcta atgtgcgaat atcatgttct agctcgttat tatttgtaaa ataatgactt
      661 aaattcaacc ttccttaaac gcacgaaaac ccacaacatg aattgtgggt cttcgcatct
      721 tgcttattta agttatttaa tttcaacatc cgcaccagct tcgacaagag cttttttgtg
      781 ttcttctgct tcaacaggtg agattttttc tttaatgttg acaggggcac catcaactaa
      841 ttttttggca tccattaagc ctaagccagt gatagcttgg acagctttaa tgactgcaac
      901 tttactttga ccccagtgtt tgagcactaa ggtgacttct gatggacctt tgtcaactgg
      961 gccatcatct tctggtgctg gtccagcagt tgcgactgct gcggtaacgc cgaattcttc
     1021 ttcgactgct ttaactaaat catttaattc gagtacggtc atttctttta atgccgcaat
     1081 aatgtctgta acagttaatt ttgccatttt tcttttcctc cttgggcaat tttattgttt
     1141 ttctgcgacc gctttaactg tagcggcaaa cttttgaatt ggtgcattga gcactgataa
     1201 gaacattgag atcattccgt tgcgatctgg taagcgtcca atgttacgta cttctgcgcc
     1261 atcgactaca cggtttcctt cgaaaagacc acctttaatt tcgacgacat cattgaagcg
     1321 tgcgaagcgt gttaatgctt tagcgcctga actaacatct ttactaaata agaatgcgtt
     1381 tggtccggtt aaataatcat ttaattcttc gcggccaagt tctttaactg cgcgttcgac
     1441 taatgagttt ttatatacac ccatcgtgtt gccatcagcg cgtaataagc gacgtaattc
     1501 agtaatttgt gaaacactta agccgtggta agtaaacacg ataaatgctt catgactttt
     1561 agctaaatcg actacttctt taacaattgc ttcctttgca acgatgcttt tttgattcat
     1621 tgttcatcct ccttccgtta atttggtaat tgaggcttca ataatcccac aaggatattc
     1681 actaatttat ataaattagt gaatatcctt gtgggattat tgaagcctca attaccaaat
     1741 taacggaagg aggatgaaca atgaatcaaa aaagcatcgt tgcaaaggaa gcaattgtta
     1801 aagaagtagt cgatttagct aaaagtcatg aagcatttat cgtgtttact taccacggct
     1861 taagtgtttc acaaattact gaattacgtc gcttattacg cgctgatggc aacacgatgg
     1921 gtgtatataa aaactcatta gtcgaacgcg cagttaaaga acttggccgc gaagaattaa
     1981 atgattattt aaccggacca aacgcattct tatttagtaa agatgttagt tcaggcgcta
     2041 aagcattaac acgcttcgca cgcttcaatg atgtcgtcga aattaaaggt ggtcttttcg
     2101 aaggaaaccg tgtagtcgat ggcgcagaag tacgtaacat tggacgctta ccagatcgca
     2161 acggaatgat ctcaatgttc ttatcagtgc tcaatgcacc aattcaaaag tttgccgcta
     2221 cagttaaagc ggtcgcagaa aaacaataaa attgcccaag gaggaaaaga aaaatggcaa
     2281 aattaactgt tacagacatt attgcggcat taaaagaaat gaccgtactc gaattaaatg
     2341 atttagttaa agcagtcgaa gaagaattcg gcgttaccgc agcagtcgca actgctggac
     2401 cagcaccaga agatgatggc ccagttgaca aaggtccatc agaagtcacc ttagtgctca
     2461 aacactgggg tcaaagtaaa gttgcagtca ttaaagctgt ccaagctatc actggcttag
     2521 gcttaatgga tgccaaaaaa ttagttgatg gtgcccctgt caacattaaa gaaaaaatct
     2581 cacctgttga agcagaagaa cacaaaaaag ctcttgtcga agctggtgcg gatgttgaaa
     2641 ttaaataact taaataagca agatgcgaag acccacaatt catgttgtgg gttttcgtgc
     2701 gtttaaggaa ggttgaattt aagtcattat tttacaaata ataacgagct agaacatgat
     2761 attcgcacat tagaattatc attacaaggc acaaatttaa cgctaattac tgatcgcgga
     2821 gtcttcagca ataaagaaat tgatgaaggg tccattgtac tactcaaaag tattattaag
     2881 agcatagacg taaagggcga agtacttgat gtcgggtgtg gttacgggac gcttggctta
     2941 tatttagccg cacgctaccc cgaaattaaa gtcacaatgt tcgacattaa tgaacgcgct
     3001 gtaagtctaa cgagtgagaa cgctaaacgc aacaacctca caaatacaca tgtttatgta
     3061 gatagtgact atcaggctct acctaaacat acattcaact tgatagttat taacccacca
     3121 attcatgcgg gtaaaaaagc ttattacccc cttcttagtg cggcgcgtga ctatctcact
     3181 gatggcggaa tactgatgtt tgtgattcgt aagtcccatg gcgctaaaag tgccgcaaag
     3241 tacttaagtg actattattc aaatatcaca cttatacaaa aagatgcggg attctacgtc
     3301 tatcacgcac aaaagtaaca aaatacaagt aaagcgaggc catttatgaa aacaattaac
     3361 gataaaacaa aaagactcca
//
LOCUS       DAOPCD010000098         3359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_22650_length_3359_cov_3.279964, whole genome shotgun sequence.
ACCESSION   DAOPCD010000098 DAOPCD010000000
VERSION     DAOPCD010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22650_length_3359_cov_3.279964"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PLQ55_03130"
     CDS             <1..376
                     /locus_tag="PLQ55_03130"
                     /inference="COORDINATES: protein motif:HMM:NF014557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPK28956.1"
                     /translation="LVSFILIIASFVQFVEMFLKRFVPALYKSLGIYLPLITTNCVVL
                     AVSLDVVATNGGVPVYNFLNMVGYAFFTGVGYAVILILFSFIQERLINNKNMPKSFQG
                     SGIALVTIGLLVMAFLGFAGMI"
     gene            378..560
                     /locus_tag="PLQ55_03135"
     CDS             378..560
                     /locus_tag="PLQ55_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28957.1"
                     /translation="MSDVARIFVAIGVMAILIALYIGSYVLNKRTKKPEGCKDFASSC
                     SGCAVTSCSHNPLKEE"
     gene            564..878
                     /locus_tag="PLQ55_03140"
     CDS             564..878
                     /locus_tag="PLQ55_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPK28958.1"
                     /translation="MQYFWGVVILLVIGALLGLLLAVASKAFAVKEDPRLEPITEMMP
                     GINCGTCGYPGCKELVVAMLKGEVKNLGQCRPLRPDAKAKIKEYLANHPDEEGNILTV
                     QG"
     gene            902..1315
                     /locus_tag="PLQ55_03145"
     CDS             902..1315
                     /locus_tag="PLQ55_03145"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HPK28959.1"
                     /translation="MKARKVKLNKTLFDTIIFSIVLVLTSATTLYLKRAFSTTEALYV
                     YVYVTDRLIDKQPLAIDNTFIYNEPEFLAPITLEIYNKKVRVEKEESPLNLCSIKGFT
                     DKVGDPVLCLPNSFYFVIMGFNYKEAPSPYVPSVE"
     gene            1296..1850
                     /locus_tag="PLQ55_03150"
     CDS             1296..1850
                     /locus_tag="PLQ55_03150"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HPK28960.1"
                     /translation="MSQVSNNGNKRKLSTQKLVRLAVFLALGVVFNIVENYITFIPAL
                     PGVKLGLANSIGLILLALYGPLEFFTIGLLRVLLSGTFSGFGTSFILSLSGFLLSSAF
                     VLLAYLFNKLSIYGLSLLSAVMHGVGQVVMIAIIYQNALMLNYLIIMTISGIISGLLI
                     ALLSKIVILRLKMFKEEECNYEIN"
     gene            1837..3147
                     /locus_tag="PLQ55_03155"
     CDS             1837..3147
                     /locus_tag="PLQ55_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPK28961.1"
                     /translation="MKLIKKLFALFSPLELVILMVSITISTLLVVPAFSKDTQKKTPI
                     LLFPTEKVYNTDGGRPIEVFDTSFFVVYEEVEEQTQNDIKAILNEYLISYHKLFDRHN
                     DYYAVEPHDNIHPTAEEKATLPRIKNLKYINEHKGEEIEIHQALYDLLVASKSYSINT
                     PNNAFSMFIGELYDYWKPYTSPGADPTKDPVRDDLKQAEIARLTSYIPQSEDDINNTL
                     KLWTNDNKYYVKFNEFNHSGDDLSISVGAVAKGMMTDILAEAMRAKGLTKGLIYGGQS
                     SFTFLEDGFMNKAYKITMEPIGPGEKTFTMSRADQYQMSTSGIYTGFKFNYGGAQIIR
                     SHIINPLTGYPAHNSHHMVNITSDTLSGLTLDYLTTALTVLSVQDGLTFLSEEYPSDD
                     INVIYSGYFNDEWFVAFTNGYPGGKTPSFQLAKEYREINLEFLL"
BASE COUNT         1026 a          539 c          677 g         1117 t
ORIGIN      
        1 actcgtgtcc tttattttaa tcattgcttc attcgtacaa tttgtagaaa tgttcttaaa
       61 acgttttgtg ccagctttat ataagagtct tggcatttat ttgccactta ttacgacaaa
      121 ctgtgtcgtg ttagcggtta gtcttgatgt tgtagccaca aatggcggag tcccagttta
      181 taatttctta aatatggttg gctatgcttt ctttactggt gttggctacg ctgtcatttt
      241 aattctcttt agttttattc aagaacgctt aattaataat aaaaatatgc cgaagagttt
      301 ccaaggtagc gggattgctt tagtaacaat tggtttacta gtgatggcct tccttggatt
      361 tgcagggatg atttaagatg agtgatgttg cgcgtatttt tgtggcaatc ggtgtgatgg
      421 cgatcttaat tgccctttat attggaagtt atgtgttaaa taaacgcaca aaaaaaccag
      481 agggttgtaa agatttcgct agtagttgta gcggatgtgc ggtgacatca tgtagtcaca
      541 atccactaaa ggaggaataa tttatgcaat atttttgggg agtagttatc cttttagtta
      601 ttggagcctt attaggactt ttacttgccg tcgcgagtaa agcttttgcg gtcaaggaag
      661 acccacgcct tgagccaatt acggagatga tgcctggtat taattgcggg acttgtggtt
      721 atccgggatg taaagaacta gtagtggcga tgcttaaggg tgaagtgaaa aatttaggtc
      781 aatgtcggcc gttgcgtcct gatgcaaaag caaagattaa agaatattta gcaaatcatc
      841 cggatgaaga agggaacatc cttaccgttc aaggataaaa agttaggtta ctcttattta
      901 catgaaggcg cgtaaagtta agttaaacaa aacacttttt gacaccatta tctttagtat
      961 tgtcttggtg ctaacaagtg cgacaacgtt gtacttaaaa cgcgcctttt ctactactga
     1021 agcactgtat gtttatgtct atgttaccga ccgtctaatt gataaacaac cgttagcgat
     1081 tgataataca ttcatttaca atgaacctga atttttagca ccaattacac ttgaaattta
     1141 caataagaaa gtgcgggttg aaaaagaaga aagtccgctt aatttatgct caattaaagg
     1201 ttttacggat aaagtaggtg atcccgtact ttgtctacct aattcttttt attttgtgat
     1261 tatgggattt aattataagg aggcaccttc accgtatgtc ccaagtgtcg aataacggaa
     1321 ataaaagaaa attatcgaca cagaagctcg tgcggcttgc tgtcttttta gcgctaggtg
     1381 tggtatttaa tattgttgaa aattacatta catttatccc ggctttaccg ggtgttaaat
     1441 taggccttgc taattcgatt ggcttaattc ttttagcact ttatggtccg cttgaatttt
     1501 tcacgattgg cttacttcga gttttattaa gcgggacttt tagtgggttt ggcactagtt
     1561 ttattttatc gcttagtggt tttttacttt caagcgcttt tgtactatta gcctatcttt
     1621 ttaataaatt atcaatctac ggactctcac tccttagtgc ggtgatgcac ggtgttggcc
     1681 aggtcgtcat gattgcgata atttatcaaa acgctttaat gcttaattat ttaataatta
     1741 tgacaataag tgggattata agcggattac ttattgctct cttatcaaaa attgtgattt
     1801 tgagacttaa aatgtttaag gaggaagagt gtaattatga aattaattaa aaaactcttt
     1861 gcgctttttt ccccgcttga actagttatt ttaatggttt caattacaat ctcaaccctt
     1921 ttagttgttc cagcgtttag caaagatacg caaaaaaaga caccgatttt attatttccg
     1981 acagagaaag tttataacac tgatggtggg cgtcccattg aagtattcga cactagtttt
     2041 ttcgtcgtgt atgaagaagt ggaagaacaa acacaaaatg acattaaagc tattctaaat
     2101 gaatacttaa ttagttacca taaacttttt gaccgtcata atgattatta tgcagttgaa
     2161 ccccacgaca atattcatcc gacagcggaa gaaaaagcga cactgccacg cattaaaaac
     2221 ttaaagtata ttaatgaaca taaaggcgaa gaaattgaaa ttcatcaagc tctttatgac
     2281 ttacttgttg cgtcaaaaag ttattctatt aacacaccaa ataatgcttt tagtatgttt
     2341 attggtgaac tttacgatta ctggaaacct tacactagtc cgggggctga cccgactaaa
     2401 gatccagtgc gtgacgattt aaaacaagcg gaaattgcac ggttaacgag ttatatcccg
     2461 caaagtgaag acgatattaa taacacttta aagttatgga ctaacgataa taaatactat
     2521 gttaaattta atgaatttaa tcatagcggt gacgatcttt cgatttctgt tggtgcggta
     2581 gctaaaggga tgatgactga tattctagcc gaagctatgc gtgctaaagg actaactaaa
     2641 ggtttgattt acggcggaca atcgtcattt acatttttag aagacggatt tatgaataaa
     2701 gcttataaga ttacgatgga acctattgga ccaggcgaaa aaacatttac aatgagccgc
     2761 gctgatcaat accaaatgtc gacaagtggg atatatacgg gctttaaatt taattatggt
     2821 ggtgcgcaaa ttattcgttc gcatattatt aatccgctaa ctggttatcc tgcgcataac
     2881 tctcatcata tggttaacat tacaagtgac acgctaagcg ggttaacgct tgattaccta
     2941 acaacagcat taaccgtact tagtgttcaa gatggattaa catttttaag tgaagagtat
     3001 cctagtgatg acataaatgt gatttatagt ggttacttta atgacgaatg gtttgtggcc
     3061 tttacaaacg gttatccagg cgggaaaaca ccatcatttc agttagcgaa agaataccgc
     3121 gaaataaatc tcgaattctt actataaagg ttgcttttct taataatatt tattaagatt
     3181 atatacgcag taagaagaaa atagaggaga atgatatata tgaaaaaaag aaacttatta
     3241 ctagcattag tcggtagtgc cttactttta agtggctgta acgctggtgg aaaacagtat
     3301 tttggttttg gtagcgttgc ttcttatgat gatgtcgatt cctatggtca gcaccttga
//
LOCUS       DAOPCD010000099         3334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_22854_length_3334_cov_3.578225, whole genome shotgun sequence.
ACCESSION   DAOPCD010000099 DAOPCD010000000
VERSION     DAOPCD010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22854_length_3334_cov_3.578225"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3314)
                     /locus_tag="PLQ55_03160"
     CDS             complement(<1..3314)
                     /locus_tag="PLQ55_03160"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPK28962.1"
                     /translation="MKTINDKTKRLHNDRVDFSKISGNFEVPNLVEIQIDSFEWFKNE
                     GIPEVFNEIFPVESYSKDIEIGFEGFRFDTPKYTYLECKDRGFTYSAPLRVTLSLGIR
                     KTGEVVKSEIFMGDIPLMTHTGTFVINGAERVIVSQIVRSPGAYMSKELDTKSGKFIY
                     NADLNPTRGTWLNFENDAKGLLSVRIDKQRKMNATLLLKALGITDYHETIELFDVFDA
                     KNRAKNNIRKSEEEMTSLELTLRKDESFTTSLEALYEIFRRLKPGEPMSDEGVKNHLI
                     QKFFDPKRYDLGKAGRFKFAKKLGIYERLEGVVLAEDLVSVDGEILFEKEHQMKKEDV
                     DKLRALKFFEKGAHTYTIPFNPDLDDYNTVNIVKAYVNDEKTQAINIVGTDLNIDEKR
                     VTTPDIVAVFSYFLNLMHGIGRVDDIDHLSNRRVRPVGELIQNQFRVGLSKMVKAMKD
                     SSSIADTETITVQKLVNTRAVASAIRDFFATSQLSQFMDQVNPLAELTNKRRISALGP
                     GGLTRDRASFDARDVHYSHYGRICPVETPEGQNIGLINNLACYAKVNEYGFIETPYRK
                     VLKPYGQITNETIYLDADEEKEFVIAEANVEVDENGVIQTQEIIARYQGENVLVSPDK
                     VDYIDVSPKQIVSIAAASIPFLENDDAMRALMGANMQRQALPLMVPESPYVGTGLETK
                     IAHDSGSAITAHKDAVVTYVDGANISVKFDDDTTFTYKLQKFARSNQGTCFNQVPIVK
                     VGDKVKKGQVLADGPSMVNGELSLGRNVTIAFMTWDGYNFEDAVIMSDRLVKEDVYTS
                     IHIEEYKIEARKIKTVSERITRDIPNVSEAAKAFLDENGIIIPGAEVKEGDILVGRVT
                     PKGQREQSPEDRLLQTIFNEKVREVKDTSLRVPHGGGGTVIDVKVFTREAGDELGPDV
                     EISVSVFIGQKRKISEGDKMSGRHGNKGVISRILPEADMPFMEDGTPIDIMLNPLGVP
                     SRMNIGQVLEIHLGMACKKLGVKVASPVFDGMTNEEIFELMEEAKMHKDGKEILYDGR
                     TGDRFDERISVGVMYLIKLVHMVDDKLHARATGPYSLVTQQPLGGKAQNGGQRFGEME
                     VWALEAYGAANILQEIL"
BASE COUNT          919 a          723 c          616 g         1076 t
ORIGIN      
        1 agaatttctt ggagaatgtt agcggcacca taagcttcaa gtgcccacac ttccatttcc
       61 ccaaatcttt gtccaccgtt ttgcgcttta ccaccaagtg gttgttgtgt gacaagacta
      121 taaggtccag tcgcacgcgc gtgcaactta tcatcaacca tgtggacaag tttaattaag
      181 tacatgacac caacactaat ccgttcatca aaacggtcac cggtgcggcc gtcatatagt
      241 atttctttac cgtctttatg catctttgct tcttccatta attcaaagat ttcttcatta
      301 gtcatcccgt caaatactgg agaagcaact ttaacaccga gtttcttaca agccatacct
      361 aagtgaattt caagtacttg accgatattc atccggcttg gtacaccaag ggggttaagc
      421 ataatatcaa ttggtgtgcc atcttccata aacggcatat cagcttctgg gagaatccgc
      481 gaaataacac ctttattacc atggcgtccg ctcattttgt ccccttcact aattttacgt
      541 ttttggccga taaagacact gacactaatt tcgacgtctg gtcctagttc gtcgcctgct
      601 tcacgggtaa agactttaac gtcaatcacc gttccgccgc caccatgcgg gacacgtaag
      661 gaagtgtctt taacttcacg cactttttca ttaaagattg tttgtagtaa gcgatcttct
      721 ggactttgtt cacgttgacc ttttggtgtc acgcgaccaa ctaagatatc gccttcttta
      781 acttccgcac caggaataat aataccgttt tcatctaaga acgcttttgc ggcttcactc
      841 acatttggaa tgtcgcgcgt aatccgttca gaaacagttt taattttccg ggcttcaatt
      901 ttatattctt caatatggat actcgtatac acatcttctt taacgagtct gtcactcata
      961 ataaccgcgt cttcaaagtt gtaaccatcc cacgtcataa aggcgatagt aacattacga
     1021 ccaagggaga gttcaccgtt aaccatcgat ggtccatcgg ctaaaacttg accttttttc
     1081 accttatcac caactttaac gattggtact tggttaaagc aagttccttg gttactccgc
     1141 gcgaattttt gtaatttata agtgaaagtt gtgtcgtcat caaacttcac actaatgtta
     1201 gcgccgtcta cataagtgac aacggcatct ttatgtgcag taattgctga ccccgagtcg
     1261 tgagcaattt ttgtttcaag tcccgtacca acatatggtg attctggcac cattaatggt
     1321 agcgcttgtc gttgcatgtt agcacccatg agtgcccgca ttgcgtcgtc attttctaaa
     1381 aacggaattg acgcagcggc gatactcacg atttgttttg gtgagacatc aatgtaatca
     1441 actttatcgg gactaactaa gacgttttca ccttgataac gggcgatgat ttcttgagtt
     1501 tgaatcacac cattttcatc aacttcaacg tttgcttcgg caataacgaa ctctttttct
     1561 tcgtccgcgt ctaaataaat tgtttcgttt gtaatttgac catatggttt taagacttta
     1621 cgatatggtg tttcaataaa gccatattca ttaactttcg cataacacgc taggttatta
     1681 ataagcccaa tattttgtcc ttctggcgtt tcaactggac aaatacgacc atagtgcgaa
     1741 tagtgaacgt cacgcgcatc aaagcttgcg cggtcacggg taagtccgcc aggacctaag
     1801 gcactaatac gccgtttgtt tgtaagttcc gcaagtgggt taacttggtc cataaattgc
     1861 gaaagctgac ttgtcgcgaa gaaatcacga atcgcactcg ctactgctcg agtattgaca
     1921 agtttttgga cagtaatagt ttctgtatca gcaattgatg aagaatcttt cattgcttta
     1981 accatttttg aaagtccaac acggaattga ttttgaatta attcgccaac tggacgaaca
     2041 cgtctattgc taaggtggtc aatatcgtca acgcgaccaa taccgtgcat tagatttaag
     2101 aaataggaga aaacagcgac aatatcagga gtcgtgacgc gtttttcgtc aatatttaaa
     2161 tcagtgccaa caatattaat tgcttgtgtt ttttcatcat taacataagc cttaacaata
     2221 tttacagtat tataatcatc taaatcaggg ttaaaaggaa tcgtataagt atgggcacct
     2281 ttttcgaaga atttaagggc acggagctta tcgacatctt cttttttcat ttggtgttct
     2341 ttttcgaaaa gaatttcgcc gtcaacactt actaaatctt cagcaaggac aacgccttct
     2401 aaacgttcgt agataccaag ttttttcgca aatttaaagc gacccgcttt gcctaagtca
     2461 taacgctttg gatcaaagaa tttttgaata aggtgatttt taaccccttc atcactcatt
     2521 ggttcgcctg gttttaagcg ccggaaaatt tcgtaaagtg cttcaagtga agttgtaaaa
     2581 ctttcgtctt tacgcaaagt taattcaagc gaagtcatct cttcttccga tttgcgtata
     2641 ttatttttgg cgcggttttt cgcgtcaaac acatcaaaga gttcaattgt ctcgtgataa
     2701 tcggtaatgc caagtgcctt taaaagcaac gttgcattca ttttccgttg tttatcaatc
     2761 cggactgata ataaaccttt agcgtcattt tcaaagttta accatgtccc gcgcgttgga
     2821 tttaaatcag cgttataaat aaacttacct gatttagtgt ctaactcttt ggacatatag
     2881 gcgcctggtg aacgaacaat ttgcgagacg attactcgtt cagcaccgtt aataacgaat
     2941 gtaccggtat gtgtcattaa agggatatca cccataaaga tttcgctttt aacgacttca
     3001 cccgtttttc taatcccaag cgataatgta acgcgtaatg gtgcgctata agtaaagcca
     3061 cgatctttac attctaaata cgtatatttt ggagtatcaa agcggaatcc ttcaaagcca
     3121 atttcaatgt ctttgctata agattcaaca gggaagattt cattaaatac ttccgggata
     3181 ccttcatttt tgaaccactc gaatgagtcg atttgaattt ctacaagatt aggaacttcg
     3241 aagtttccgc tgatcttgct aaagtcaact cggtcattgt ggagtctttt tgttttatcg
     3301 ttaattgttt tcataaatgg cctcgcttta cttg
//
LOCUS       DAOPCD010000100         3325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_22920_length_3325_cov_3.080428, whole genome shotgun sequence.
ACCESSION   DAOPCD010000100 DAOPCD010000000
VERSION     DAOPCD010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22920_length_3325_cov_3.080428"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1672)
                     /locus_tag="PLQ55_03165"
     CDS             complement(<1..1672)
                     /locus_tag="PLQ55_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPK28963.1"
                     /translation="MNFNPFPNYEQDEKILEKFGRFLDEDAKLNKSDPVIGRDSEIRR
                     IIEILARKQKNNVILLGEPGVGKTAIIEGLAQRIVAGDVPKNLADKRIYELDMGALVA
                     GAKFRGEFEERLKAILKKIKESNHQIILFIDEIHLIVGAGKAEGAIDAGNMLKPMLAR
                     GEIHTIGATTLDEYRTYIEKDRALERRFQPLIVQEPTLEDTISILRGLKARYEAHHGV
                     KISDNALVSAAVLSDRYITERFLPDKALDLVDEACALIRVEVESLPAELDEIRRKMRQ
                     LEIERVALSKENDDLSKVRLQKVNEELENLKKEDEAFSKEWQHELDELNVLGKAKKEL
                     EQARFNLSVAFNSGNYEEASTLQYKVIPELEAKIETLTKETKDNKVLKETVTENEIAA
                     VVSRATGIPLKRIMESQKEKILSLYDELKKRVLGQDVALRLVSDAILRQRAGLNNPNR
                     PIGSFLFLGPTGVGKTEVAKSLAYALFDDERHIVRIDMSEYMEKFSVSRLIGAPPGYV
                     GYDEGGQLTEAVRRSPYSIILFDEIEKAHPEVFNLLLQLLDDGRLTDSQ"
     gene            complement(1782..2807)
                     /locus_tag="PLQ55_03170"
     CDS             complement(1782..2807)
                     /locus_tag="PLQ55_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28964.1"
                     /translation="MGTNSKFNLGINLTGDSDLDRHNIFNYIIENFTKDKTEAEILVL
                     AATFLGKDTKTIFNEIRVNPEVLVPVCLYLMSEEKIALSRRNEIGNALLNYTDAHARK
                     IDDKATRKPLYNFTLEYEKALYTKMANSNVEDILVTFPFYRIYYTQGLIYEAMADYTK
                     AFVMYSKAHQWNPFNPKPILKILEGLKYDGRAQDLLRLSMWYLQITYSAPNISAALRY
                     AGYALYLDGKFAEAYAFYYQAIVYQDGETPARFNEEITSVLTALKKDAPYELSRAQIK
                     KLFAYSKFKPYPSEIAFDTIRPLIINHYTKGNYVDAIRYATQYVIFRPNDDKIMRILK
                     ASKLNLD"
     gene            complement(2874..>3325)
                     /locus_tag="PLQ55_03175"
     CDS             complement(2874..>3325)
                     /locus_tag="PLQ55_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HPK28965.1"
                     /translation="HAAVTPIVVKEKHKYIMYLILRNNRTDEKYPTGIFHAHPEYHNI
                     KKEGIGLIEQMGTFILPARLKNEFGLIEELYKKGVTGAEMISEHPSIAKHATFIKELM
                     LSKIKPTEFEKYARDYMTNVCENILQNTACFKNDEAGQDAFIKFLQL"
BASE COUNT          953 a          666 c          550 g         1156 t
ORIGIN      
        1 cttgagaatc agttaagcgg ccatcatcta gtagttgtaa gagtaaatta aagacttcag
       61 gatgcgcctt ttcgatttca tcaaataata taattgaata aggactacgc cgcactgctt
      121 cggttaattg cccgccctca tcataaccaa cgtatcccgg tggggcacca ataagacgcg
      181 agacactaaa tttttccata tattcactca tatcaatccg gacaatatgg cgttcgtcgt
      241 caaaaagagc gtaggctaag cttttggcta cttcggtctt accaacccct gttggaccta
      301 ggaataagaa actaccaatt gggcgatttg ggttattaag tcctgcgcgt tgccgtaaaa
      361 tggcatcact aacaagccgc agggctacat cttgccctaa tacgcgtttt tttagttcat
      421 cataaagcga taaaatcttt tctttttggc tttccataat tcgttttaac ggaatccctg
      481 ttgcgcgtga gacgaccgca gcaatttcat tttccgttac tgtttctttt aatactttat
      541 tatctttagt ttcttttgtt aaagtttcga tctttgcttc aagttcagga attactttat
      601 attgtaatgt tgaagcttct tcgtaattac cgctattaaa ggccacactt aagttaaagc
      661 gtgcttgctc taattctttt ttagccttac caagcacatt aagttcatct aattcatgtt
      721 gccattcttt actaaaggct tcatcttctt ttttaagatt ttccagttct tcatttactt
      781 tttgtaaacg aactttactt aagtcatcat tttctttact aagtgcaact cgttcaattt
      841 caagttgccg catcttccgg cgaatttcat cgagttcggc gggtaaactt tcaacttcaa
      901 cacgaattaa tgcacaagct tcgtccacta agtcaagcgc cttgtcgggt aaaaagcgtt
      961 cggtaatata acggtcacta agtactgctg cactcacaag tgcattatcg cttatcttaa
     1021 cgccgtgatg ggcttcgtaa cgcgctttta gtccgcgtaa tatcgaaatt gtatcttcaa
     1081 gcgtcggttc ttggacaata agcggttgga atcttctttc aagcgctcgg tctttttcaa
     1141 tgtaagtgcg atattcgtca agtgtcgtcg ccccaatcgt atgaatttca ccgcgcgcta
     1201 acattggttt aagcatatta ccagcgtcaa tggctccttc ggctttacca gcgccgacga
     1261 ttaaatgaat ctcatcaata aataaaatga tttgatgatt actttcttta attttcttta
     1321 aaatggcttt gagccgctct tcaaattcac cgcggaactt cgcaccagcc actagtgcgc
     1381 ccatatctaa ttcatagatt cttttatctg ctaaattctt tggcacatca ccagcaacga
     1441 ttcgttgcgc tagtccttca ataatagcgg tcttaccgac ccctggttca ccaagtaaaa
     1501 taacattatt tttttgcttt ctcgctaaaa tttcaataat gcgtctaatt tcactatcac
     1561 gcccaattac tgggtcactt ttatttagtt tagcgtcttc atctaaaaag cgtccaaact
     1621 tctctaagat tttttcatct tgttcataat ttggaaaagg attaaaattc ataatataac
     1681 acctccttag cactttaaat gtctgagtgc taagtctatt atatactaaa attggcactt
     1741 gtcaacaccg agtgccattt tttgtaatat ttttaatatt attagtctaa attaagttta
     1801 cttgctttta aaatgcgcat tatcttatca tcatttggac ggaaaataac atactgtgtc
     1861 gcataacgaa tagcatcaac ataattacct tttgtataat gattaataat tagtggtcta
     1921 atcgtatcaa atgctatttc actaggatac ggtttaaatt ttgaatacgc aaaaagtttt
     1981 ttaatttgtg cccggcttaa ttcgtatggg gcatcttttt tcaaggcagt taatacactt
     2041 gtaatttctt cattaaaacg tgctggtgtt tcaccatctt gatacacgat tgcttggtaa
     2101 taaaaggcat aagcttcggc gaacttacca tcaagataaa gcgcatatcc tgcataacgc
     2161 aatgccgcac taatgtttgg ggcgctataa gtaatttgca agtaccacat tgaaagccgt
     2221 aataaatctt gagcgcgacc atcatacttt aaaccttcaa ggattttaag aattggttta
     2281 ggattaaacg gattccattg atgagctttt gaatacatca caaatgcttt cgtgtaatcg
     2341 gccattgctt cgtaaatcaa gccttgcgta taataaatac ggtaaaaagg gaatgtcacc
     2401 aaaatatctt caacatttga attagccatc ttcgtgtata acgctttttc gtattcaagc
     2461 gtaaagttat aaagtggttt gcgtgtcgct ttatcatcaa tcttccgcgc atgtgcgtcg
     2521 gtgtaattaa gcaaagcatt accaatttca ttacgccgtg atagcgcaat tttttcttca
     2581 ctcattaaat ataaacatac agggacaagt acttcagggt taacacgaat ttcattaaaa
     2641 atcgtttttg tgtctttacc taaaaaagtc gccgctaata ctaaaatctc cgcttcagtt
     2701 ttatctttgg tgaagttttc aataatataa ttaaaaatat tatgacgatc aagatcgcta
     2761 tcaccagtta agttaatccc taagttaaat ttactgtttg ttcccataac tcaatgttaa
     2821 caaaaaagtg gcattgtcgc cacttttaac actttaattc gtgcaattct ttattaaagt
     2881 tgtaaaaact taataaaggc gtcttgtcca gcttcatcat ttttaaagca tgcggtattt
     2941 tgtaaaatgt tttcacagac attagtcata taatcacgcg catacttttc aaattcagtt
     3001 ggtttaattt tactgagcat taattcttta ataaaagtag cgtgtttagc aatacttggg
     3061 tgttcactaa tcatttcggc accagtaaca ccttttttat ataattcttc aattaaacca
     3121 aattcattct ttagtcgcgc tggtaaaata aaggttccca tttgttcaat taggccaatc
     3181 ccttcttttt taatgttatg gtattcggga tgagcgtgga atataccggt tggatacttc
     3241 tcatcagtgc gattattacg taaaattaag tacataatgt atttatgttt ttctttaaca
     3301 acaattggtg tgaccgcggc gtggc
//
LOCUS       DAOPCD010000101         3313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_23025_length_3313_cov_2.542664, whole genome shotgun sequence.
ACCESSION   DAOPCD010000101 DAOPCD010000000
VERSION     DAOPCD010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23025_length_3313_cov_2.542664"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1416
                     /locus_tag="PLQ55_03180"
     CDS             115..1416
                     /locus_tag="PLQ55_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HPK28966.1"
                     /translation="MKLFQTVGRRKISGYKSMSLERELVVLDPKKVEYVYFPLVAANN
                     VPLEVFVKEGDKVKVGTKIALKQQFSVPLFSSVSGTVRAIEKRLNVTTGRPCDHLVIK
                     NDFKMTKEKPLKKVELDASKEDIVAAIKEAGIVGLGGANFPTWVKYNNVKDIHTILIN
                     AVECEPYLTTDYVSCSLNAAKVISGARYLQKAAGASKVIVAIKVKKEQACNCLKEAAA
                     PYPDIEVREVPDRYPMGWERTLIQTIFKKQYDKLPSEIGVIVNNSTTAIAVHDALVEG
                     SPIVSRIVTVSGNAVKNAANIVVPVGILAETVLNEFEVEEKEVVLLVGGPMTSNPAKD
                     VSFALTVCHGALTLLEKVVYRPVACLRCGSCTAHCPAGLQPIEILRAFEGRDYERLEK
                     LETLRCVDCGLCSYVCPSKIEVTDMMKKAKTLLRVHQSRKK"
     gene            1428..3263
                     /locus_tag="PLQ55_03185"
     CDS             1428..3263
                     /locus_tag="PLQ55_03185"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPK28967.1"
                     /translation="MKIVSGNAPFLRSKRTTSQIMMELLIGLAIIFVAAVIYNFTLGV
                     RYGLKTICIMTVAILFTFISDIIAGSLRYNKEKDGKFDEYLIHFIKSNYSVVTAVIFA
                     LTLPIGTPVYVVIIGSMFATLVVKHAFGGFGSNVFNPAALSRIFLALTFGSQLKAYLP
                     GGENIGGLVAGATVTSQYAADGVKFLTGSLANTNVSMLDLYLGNHSGAIGETFTLLIL
                     IVGIVLAAREVINWRTPVFFLGTVALSSVFVSLVAKVNMIEYLLVQFGVGGMVFGAVF
                     MFTDPVTSPTSNYGKALIGIIGALINVLIRIAGVYPEGTAFSIAFVNIISPMIDRLIT
                     GRTNNKVWVPATVSGVMVLGSVGLITGVSAAKMPRVAPEPPPIVEPFVTYLGSYTSPT
                     PSEYAEEFTTNVEVGLSDAYHIVKIEYDVIANPDNYKKVTDGIDAVIDYYTSISVAEF
                     KALPVPALSEGTTGSTVVGQTGEHIIPGAIYSSRALYEAINDALKYLNVYSADVEDYP
                     YDSGGYSDETLVMSVDVYVDTVTDLIKVVDIKDGASTGTYADRWETVKEAVLSKYHDL
                     DVATFLGYTNVAELYGEEVATYAGVTYSARRLFNAVQKALEGYGG"
BASE COUNT          970 a          557 c          736 g         1050 t
ORIGIN      
        1 aataaacata cattcattat ttatggtcgt ttcacttaaa agtgaactat ttacacattt
       61 actttacttt ttgctcttta cgcgtgtata atatgcgcga tggagggaaa agtgatgaag
      121 ttattccaaa ctgtaggtcg gcggaaaatt tctggctata aaagtatgag tcttgaacgc
      181 gaacttgttg ttctcgaccc taaaaaagtt gaatatgttt attttccgct tgtagccgct
      241 aataatgtgc cgcttgaagt tttcgtcaaa gaaggcgata aagtaaaagt aggaacaaaa
      301 attgcactta aacagcaatt tagtgttccg ctttttagta gtgtctcggg aactgttcga
      361 gcaatcgaaa aacgcttgaa tgttactacg ggacgtcctt gtgatcattt agtcattaaa
      421 aatgatttca aaatgactaa agaaaaacca ttaaagaaag ttgaattaga tgcatcaaaa
      481 gaagatattg ttgcagcaat taaagaagct ggtattgtcg gtcttggtgg cgctaatttt
      541 ccaacatggg ttaaatacaa caatgtcaaa gatattcaca ctatcttaat taatgctgtt
      601 gaatgtgaac cgtatttaac aacggactac gtgtcttgta gtcttaatgc cgctaaagtt
      661 attagtggtg ctcgttattt acaaaaagcc gcgggagcaa gtaaagttat tgtggcaatt
      721 aaagtaaaaa aagaacaagc ttgcaattgc ttaaaggaag cggcagcccc ttaccctgac
      781 attgaagtgc gtgaagtacc agatcgttat ccaatgggct gggagcgcac acttattcaa
      841 acgatattta aaaaacaata tgataaatta ccaagtgaaa tcggtgttat cgttaataac
      901 agcactaccg ctattgcggt ccatgatgcg cttgttgaag gttcaccgat tgtgtcacgg
      961 attgtgacag taagtggtaa cgccgtgaaa aatgccgcaa atattgtcgt gcctgtcggt
     1021 attttagccg aaacagtgct taatgaattt gaggttgaag aaaaagaagt ggtgctatta
     1081 gtcggtggtc caatgacaag taatcctgca aaagatgtaa gttttgccct tactgtgtgt
     1141 catggtgcct tgacactact agaaaaagtc gtttatcgcc cagtagcatg tttgcgctgt
     1201 ggcagttgta cagcacactg tccagccgga ctccaaccaa tcgaaatctt gcgcgccttt
     1261 gaaggtcgtg attatgagcg acttgaaaaa cttgaaacat tacgctgtgt tgattgcgga
     1321 ctttgtagtt atgtgtgtcc gagtaaaatc gaagttaccg atatgatgaa aaaagctaag
     1381 accttacttc gcgttcatca atcgaggaag aaatagaggt gtctaatatg aaaattgtta
     1441 gtggaaatgc cccgttctta cgcagtaaac gcactactag ccaaataatg atggaactct
     1501 taatcgggtt agcgattatt tttgtcgccg ccgtcattta taactttacg cttggagtac
     1561 gttacggtct taaaacaatt tgtattatga ctgttgcaat ccttttcact tttattagtg
     1621 atattattgc tggtagttta cgttataaca aagaaaaaga cgggaagttt gatgaatatt
     1681 taattcattt catcaaatca aattatagtg ttgttacagc agttatcttt gctttaactc
     1741 taccaattgg caccccagta tatgtcgtga ttattgggag tatgtttgcc actttagtcg
     1801 taaaacatgc ctttggtggg tttggtagta atgtctttaa ccctgctgct ttgtcgcgca
     1861 tctttttggc attgactttt ggtagccaac ttaaagctta tttacctggt ggtgaaaata
     1921 ttggtggctt agtagcgggt gcgactgtga ctagtcaata cgctgctgat ggcgttaaat
     1981 tcttaactgg tagtttagca aacactaatg tgtcaatgct tgacttatat ttaggtaatc
     2041 atagcggcgc gattggtgaa acatttacct tacttatttt aattgtcggt attgtcttag
     2101 ccgcccgtga agtaattaac tggcgcactc cagtcttctt cttaggcaca gttgcactta
     2161 gcagtgtctt tgttagcctc gtagcgaaag tgaatatgat tgaatattta ttagtgcaat
     2221 ttggtgttgg cgggatggta tttggtgctg tcttcatgtt tactgatcca gttacttcac
     2281 caacgagcaa ctacggtaaa gcgctgattg gaattattgg ggcattaatt aatgtcttaa
     2341 ttcggattgc tggtgtttat ccagaaggta cagcttttag tattgcattt gttaacatca
     2401 tttcaccaat gattgaccgt ttaattacgg gccggacaaa taataaagta tgggttcctg
     2461 ctactgttag tggggtgatg gtattaggta gtgttggctt aattaccggc gttagtgcgg
     2521 caaagatgcc acgcgtcgcg ccagaaccac cgccgatcgt tgaaccgttt gtgacttatt
     2581 taggaagtta tacaagtccg acaccaagtg aatacgctga ggaatttacg acaaatgtcg
     2641 aagttggatt aagtgatgct tatcatattg taaaaattga atatgatgtg attgcgaatc
     2701 cggataatta caaaaaagta actgatggga ttgatgcggt cattgattac tatacctcaa
     2761 ttagtgtcgc cgaatttaaa gccttaccag tcccagcttt aagcgaaggt acaaccggct
     2821 ccactgttgt tggtcaaaca ggcgagcata ttatcccagg agcgatttat tcttctcgcg
     2881 cactttacga agcaattaat gatgcactta aatacttaaa tgtgtatagt gctgatgttg
     2941 aagactaccc ttatgatagc ggtggttata gtgatgaaac attagtgatg agtgttgatg
     3001 tttatgttga cactgtgact gatttaataa aagttgtgga tattaaagat ggcgctagta
     3061 ctggtactta cgctgatcgt tgggaaacgg taaaagaagc agtactaagc aagtatcatg
     3121 atttagatgt tgccacattt ttaggttaca caaatgttgc tgaactttat ggtgaagaag
     3181 tagcaactta cgctggtgta acttattcgg caagacggct ctttaatgcc gtgcaaaaag
     3241 cactagaagg atatggtggc taatatgagt aagaaaaaat taaataactt tattttattg
     3301 ccgctctttt tag
//
LOCUS       DAOPCD010000102         3308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_23057_length_3308_cov_3.938211, whole genome shotgun sequence.
ACCESSION   DAOPCD010000102 DAOPCD010000000
VERSION     DAOPCD010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23057_length_3308_cov_3.938211"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /gene="rplC"
                     /locus_tag="PLQ55_03190"
     CDS             complement(<1..444)
                     /gene="rplC"
                     /locus_tag="PLQ55_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007203551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPK28968.1"
                     /translation="MKAIIGRKMGMTQVFAEDGTIYPVTVVEVLPNVVTQVKTVEKDG
                     YAAIQVGYQDKKPSRVNKPLKGIYEKAGVTPKYGLYELKGEEMLSYEVGSEIKVDIFK
                     KGDIVDVTGTSKGKGYTGVIKRYGFKLGNAAHGSGYHRGIGSMASV"
     gene            complement(457..768)
                     /gene="rpsJ"
                     /locus_tag="PLQ55_03195"
     CDS             complement(457..768)
                     /gene="rpsJ"
                     /locus_tag="PLQ55_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPK28969.1"
                     /translation="MAGKQKIRVRLQSYDHALLDEAVAKIVEQAVESGAKVVGPIPLP
                     TQREVYTVLRAVHKYKDAREQFEIRTHKRLIDIVDPNKRTVEFLRKFELPSGVDIDIK
                     L"
     gene            complement(1096..1758)
                     /locus_tag="PLQ55_03200"
     CDS             complement(1096..1758)
                     /locus_tag="PLQ55_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HPK28970.1"
                     /translation="MEKINYYNISIAEVNNFKDQGLRPKLALHACCAPCSSHTLEFLT
                     PFFAVTIVYQNSNIYPKAEFKKRRDEVERFVREFNIKNNQDVKVVFLPYDHEAFMKEL
                     RVFASEKEGGKRCHLCYEKRIVEALKYGSENGFDYATTTLTISRQKNSQVINAIAKKI
                     APRYPNIKYFYSDFKKKAGLLRVREMKKEYNLYQQEYCGCEYSYRDTVLARNNKKDTE
                     NR"
     gene            complement(1745..2779)
                     /gene="queA"
                     /locus_tag="PLQ55_03205"
     CDS             complement(1745..2779)
                     /gene="queA"
                     /locus_tag="PLQ55_03205"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004801206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPK28971.1"
                     /translation="MDVNLFDFDLPKELIAQYPSLLRQNARLLAFNKDKNEIKDEHFY
                     DITKYFKHGDVLVINNTKVIPARLRGIKVPTGAKIEVVLLKALKDDEYEVLIGNAKAV
                     KVGTVVSFGDGELLMECREKKGEGIHIVKFKYEGVFLEVLERVGTTPLPPYIEDKSEK
                     YARYQTVYAKAPGSAAAPTAGFHFTPEILDELKTKGVIVVEITLHIGLGTFKPVSVSD
                     TKDHKMHYETYEISEEAAATINKAIEEKRRIISVGTTATRTLEANFNKYQKIVARSEA
                     TNLFITPGYKFKVISALITNFHLPKSTLIMLVSAFAGRENTLAAYCHAIKERYRFFSF
                     GDVMYIYGED"
     gene            complement(2779..>3308)
                     /locus_tag="PLQ55_03210"
     CDS             complement(2779..>3308)
                     /locus_tag="PLQ55_03210"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPK28972.1"
                     /translation="YSAMETVKIGFPGYAIGGTSIGEPKDVFIKMVESSVPYLPKDKP
                     RYLMGIGSIDMILQGVARGVDMFDCVLPTRIARHGSLYTRKGRLNIRRAEFKDDFRPI
                     EEECDCYTCKNYTRAYLRHLYVSEEALAQRLLSIHNLRFLIKLMEDVRVAIKENRFLD
                     FKSDVLKKFGDKRGF"
BASE COUNT          937 a          704 c          547 g         1120 t
ORIGIN      
        1 aacgctggcc attgagccaa ttccgcggtg ataaccagaa ccgtgagcgg catttccaag
       61 tttgaagcca tagcgtttaa tgacaccagt gtagcctttc cctttgcttg ttccagttac
      121 gtcgacgata tctccttttt tgaagatgtc gactttaatt tcgctaccaa cttcgtagct
      181 aagcatttct tcacccttta attcgtataa accgtatttt ggagtgacac ctgctttttc
      241 gtatatgcct ttaagtggtt tatttacccg gctcggtttt ttatcttgat agcctacttg
      301 gatggcggca tagccatctt tttccactgt tttcacttga gtgacaacat ttggtaagac
      361 ttcaacaact gtaacggggt agattgtgcc atcttcggcg aatacttggg tcatacccat
      421 tttgcggcca ataattgctt tcatatttct acctccttat agtttaatat cgatgtcgac
      481 accacttggg agttcaaact tccgtaagaa ttccactgtg cgtttgtttg gatcgacgat
      541 atcgataagc cgtttgtgtg ttctaatttc gaattgctca cgcgcatctt tgtacttgtg
      601 gaccgctcgt aacaccgtgt acacctcacg ttgtgttggg agcgggatgg ggccaactac
      661 cttggcgcca ctttcgactg cttgttcaac gatcttcgct actgcttcat caagtaatgc
      721 atgatcataa ctttgaagtc gaacccttat cttttgtttt cctgccatga attattttcc
      781 tccttacatc tacaggcgct aatggtcctc agctacgaat tccccgatta cacttcatga
      841 caacgcttgt aggtgtatcg gcaacctcgc atttcaacgc gaccattaac ttgttaaata
      901 atactaaaag cataatataa tgtcaaccat aaacgataaa aaatatatat ttttatatat
      961 aaggtcacaa aatcttgtga aaaaccaaat ttcaaacttt tgaacttttt accgaaaatc
     1021 gtgaaaaaaa ccacttttgc aagcggttcc attttttctt aaatactgct gtttttgtcc
     1081 aaaaatcgtg aaaaatcaac ggttttcggt gtctttttta ttatttcttg ctaaaacggt
     1141 gtcgcgataa gagtattcac aaccacaata ttcttgctga taaaggttat attctttttt
     1201 catttcacgg acacgcaata atccggcctt tttcttaaaa tcactataaa aatatttaat
     1261 atttgggtag cgaggcgcaa tctttttagc aattgcattg atgacttgtg agttcttttg
     1321 tcgcgagata gtaagcgtcg tagtggcgta atcaaatccg ttttcactac catactttaa
     1381 agcttctaca atacgttttt cataacaaag atgacagcgt ttaccgcctt ctttttcact
     1441 agcgaacact cttaattcct tcataaaggc ttcgtgatca tatggtaaaa acacgacttt
     1501 gacatcttga ttatttttaa tattaaattc tcttacgaat ctttcaactt cgtcgcgtct
     1561 tttcttaaac tcagctttgg gatagatgtt gctattttga tacacaattg tcacagcaaa
     1621 aaatggtgtt aaaaattcta gggtgtgact agaacacggc gcacagcaag catggagcgc
     1681 tagttttggt ctaagccctt gatctttgaa attattaact tctgcaatcg aaatgttgta
     1741 ataattaatc ttctccatat atatacatta catcaccaaa agagaaaaag cgatagcgtt
     1801 ctttaattgc gtggcaataa gccgcgagtg tattttcacg ccccgcaaaa gcactaacta
     1861 acataattag tgtagattta ggtaaatgaa aattcgtaat taacgcgctg atgactttaa
     1921 atttataacc tggagtaata aataaatttg ttgcttcact cctcgctaca atcttttgat
     1981 atttattaaa attagcttct agtgttcttg ttgcggttgt gccaacactg atgattcttc
     2041 gtttttcttc aatcgcttta tttattgttg cggccgcttc ttcgctaatt tcataagttt
     2101 cgtaatgcat cttatgatct ttagtatcac taacactgac cggtttaaaa gtacctaagc
     2161 caatatgcag tgtaatttcg acgactatca ctcctttagt ttttaactca tctaatattt
     2221 ccggcgtaaa gtgaaagcct gctgttggtg ctgccgcact accaggcgct ttagcgtaca
     2281 ccgtttgata acgcgcatat ttttcacttt tatcttcaat ataaggcggg agcggggttg
     2341 tcccaaccct ttctaagact tctaaaaaga caccctcata cttaaacttt acaatgtgaa
     2401 tgccttctcc ttttttctcg cggcattcca ttagaagctc gccgtcacca aaagaaacaa
     2461 cagttccaac tttaacggct ttagcattac caattaacac ttcatattca tcgtctttta
     2521 gtgcttttaa taacacaacc tcaatctttg ctcctgttgg aactttaatg cctctaagtc
     2581 tagcgggaat aacttttgta ttattaataa ctaacacatc accatgctta aaatacttag
     2641 taatatcata aaaatgttca tcttttattt catttttatc tttgttaaac gctaaaagac
     2701 gtgcattttg ccgtaaaaga gaaggatatt gcgcaattag ttcttttggt aaatcaaaat
     2761 caaaaagatt aacatccatt agaaacctct tttatcgcca aactttttta agacatcact
     2821 cttaaaatct aaaaagcgat tttctttaat cgccacacgc acatcttcca taagtttaat
     2881 taaaaagcgt aagttatgaa ttgagagtaa gcgctgcgct aaagcttctt cactaacata
     2941 taaatggcgt aaataagcac gcgtgtaatt cttacatgta tagcaatcgc actcttcttc
     3001 aattgggcgg aagtcatctt taaattcagc gcgccggata ttaagtctgc cttttcttgt
     3061 atagagcgaa ccgtgacgcg caatacgcgt tggtagtaca caatcaaaca tatcaacacc
     3121 gcgcgctaca ccttgtaaga tcatatcaat tgaaccaatc cccattaagt aacgcggttt
     3181 gtctttaggt aaataaggga cggaactttc caccatcttt ataaagacat cttttggttc
     3241 accgatgctt gttccaccaa ttgcgtaacc aggaaaccca atttttactg tttccatcgc
     3301 actatatt
//
LOCUS       DAOPCD010000103         3295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_23166_length_3295_cov_3.624383, whole genome shotgun sequence.
ACCESSION   DAOPCD010000103 DAOPCD010000000
VERSION     DAOPCD010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23166_length_3295_cov_3.624383"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLQ55_03215"
     CDS             <1..357
                     /locus_tag="PLQ55_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28973.1"
                     /translation="GVLVATQLGSTGYNKSIGGAVINRNLELLEYTKIAPIANSAFRP
                     LVNPLIIGEKDVLVFNGKNANTYFGYDAFTKKLSDIPFSVKVSLSNKKITIVHRQNRP
                     YSRILNESFLGGKHDI"
     gene            347..703
                     /locus_tag="PLQ55_03220"
     CDS             347..703
                     /locus_tag="PLQ55_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HPK28974.1"
                     /translation="MTFSEFLQQYGVYLAFVVFLAIVFLIILILVIPRLPKKGTKSVE
                     PSISKLEIITLLGGEDNIKEIALRGSRLTVSLADKSLANIEELKKHGVDRVIVMQAKL
                     VLLVNKEVASLFDELA"
     gene            complement(680..1222)
                     /locus_tag="PLQ55_03225"
     CDS             complement(680..1222)
                     /locus_tag="PLQ55_03225"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPK28975.1"
                     /translation="MFILASKSPRRQELFKLIIDDFAIIVPDIDENIPVSIKSDTAKI
                     ISKFKAYHIKAKYPEATILACDTIVVIDGELLGKPKDEDDARRMLTKLSGRKHHVISG
                     FTLVSPEFEINKNVVTEVYFNTLSPELIEAYIKSGSPFDKAGAYGIQDEEFKLVREIK
                     GSYYNVMGLPVEELRKFIKK"
     gene            complement(1224..2906)
                     /locus_tag="PLQ55_03230"
     CDS             complement(1224..2906)
                     /locus_tag="PLQ55_03230"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPK28976.1"
                     /translation="MAINSKFEMPVSIYALGGLGEVGKNMYVVEDEKNILLFDAGVLF
                     PEQDLPGIDYVIPDFAYLRDNARKIKALFITHGHEDHIGAIPFLVQVVPVPVIYAPPL
                     AAALIRHKLQMMRIKTPVNIVEYDGDTVVKFGSMTVSFFAVTHSIPDCYGFCVDTEHG
                     RVVGTGDFKIDLTPIGRQMDLGKIAELGNEGVDLFLSDSTNAEHEGFTPSERTVISAI
                     NEIFANTSGRLIISTFSSNISRIQQIVEATVNYRRKIIIIGRSMERVVSASRDLGYIK
                     IPDASIVDVGKSSTLKPEETVVLCTGSQGEPMAALSRIADGTHKDISIIPGDTVVFSS
                     SPIPGNSVGIDRVVNQLTRLGATVLTNSILRNIHSSGHPSQEELKLMLRLVRPKYFMP
                     VHGEYRMLKLHAELAAKVGVPLENSFVLKNGETLELRNGKVTRGRSFPVADIYIDGNS
                     TEGMSKAVLTDRRQLNQDGMVAVLIAVDPKKNELLVPVEIYSRGFINWSISNLLPDAE
                     KHINKVLKRHYAEKVTTFGEVKNIVKDELEPFFYDKTGRKPMIIPVIMSKGI"
     gene            complement(2992..>3295)
                     /locus_tag="PLQ55_03235"
     CDS             complement(2992..>3295)
                     /locus_tag="PLQ55_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPK28977.1"
                     /translation="INQLTDFLAAGVERDSVRRAYLRSGEGCLSVDEDHPGHVYRHYK
                     IRVKAFDILNNEEVEINTFGYPAIVLQHEIDHLHGVLFYDRINEENKVRYPDALSL"
BASE COUNT         1031 a          621 c          604 g         1039 t
ORIGIN      
        1 ggggtgttag ttgctacgca gcttggcagt acgggttaca ataaatcaat tggcggcgct
       61 gtgattaatc gtaatttaga attacttgaa tacacaaaaa tagcaccgat tgcaaacagt
      121 gcatttcggc cacttgttaa cccgcttatt attggtgaaa aagatgtgtt agtgttcaac
      181 gggaaaaatg ccaatacata ttttggttat gacgctttta ccaagaaatt aagtgacata
      241 ccttttagtg ttaaagtgag tcttagtaat aaaaaaataa caatagttca tcgtcaaaac
      301 cgtccttatt cccgtatatt aaatgaatct tttttaggag gtaaacatga catttagtga
      361 gtttttgcag caatatggtg tgtatttagc ttttgttgta tttttagcaa ttgtcttttt
      421 aatcatatta attttagtta ttccgcgttt acctaaaaag ggtacaaaaa gtgttgaacc
      481 aagcatttca aaattagaaa ttattacact tttaggtggt gaagataata ttaaagaaat
      541 agcgttacgc ggttcgcgtt taacagtatc acttgcggat aagtcgcttg caaacattga
      601 agaattaaaa aaacacggtg tcgaccgtgt aattgttatg caagcaaaac ttgttttact
      661 cgtgaataaa gaagtcgcat cactttttga tgaacttgcg taattcttct accggcagcc
      721 ccataacatt atagtaacta cctttaattt cacggacaag cttaaattct tcgtcttgaa
      781 taccataggc tcccgcttta tcaaatggtg agccgctttt tatatatgct tcaataagct
      841 caggacttaa agtgttgaaa tatacttcag taacaacatt tttgtttatt tcaaattcgg
      901 gactaacgag tgtgaagcca ctgataacat ggtgtttgcg cccacttaat ttagtgagca
      961 tccttctagc gtcatcttcg tcttttggtt taccaagtag ttcaccgtca atgaccacaa
     1021 ttgtatcgca cgctaaaata gtagcttcag ggtactttgc cttaatatga taagccttaa
     1081 atttagaaat tattttagcg gtgtcgcttt taatgctaac cggaatattt tcatcaatat
     1141 cggggacgat aattgcaaaa tcatcgataa ttaatttaaa taattcttgg cggcgcggtg
     1201 actttgacgc taggatgaac atactaaatt cctttactca tgatgacggg aataatcatt
     1261 ggtttacgac cagttttgtc ataaaagaat ggttcgagct catccttaac gatgttttta
     1321 acttcaccaa aagttgttac tttttcagca tagtggcgct ttaatacttt gttaatatgc
     1381 ttttcggcat ctggtaataa attagaaata ctccaattaa taaagccgcg actataaatt
     1441 tcaacaggca ctaaaagttc atttttcttt ggatcaacag caataagcac ggcgaccatc
     1501 ccgtcttgat ttaattgtcg tctgtcagtt aggaccgctt tactcatgcc ttcggtagaa
     1561 ttaccatcaa tataaatatc ggcgactggg aaagaacgac cacgtgttac tttgccatta
     1621 cgtaattcta aagtttcacc gttttttagt acgaaagagt tttcaagggg aacaccgacc
     1681 tttgctgcaa gttcggcatg tagtttcagc atccgatatt caccgtgaac tggcatgaaa
     1741 tattttggac gcacaaggcg caacattagt tttaattctt cttgcgaagg gtgaccagaa
     1801 ctatgaatat tacgtaaaat actgtttgtt aaaactgtcg ctccaagtct agttaattgg
     1861 ttaacaacgc ggtcaatccc gacgctatta ccgggaattg gtgaactact aaatacgaca
     1921 gtatcacctg gaataatact aatatcttta tgggtaccat cggcaatacg cgaaagtgct
     1981 gccatcggtt caccttgtga accagtacaa agcacgactg tttcttctgg ttttagtgtt
     2041 gatgacttac ctacatcgac aatactagcg tcaggaattt taatatatcc taagtcgcga
     2101 gaagcagaaa caacgcgttc cattgaacga ccaataataa tgattttacg cctataatta
     2161 actgttgctt cgacaatttg ttggatacga ctgatgttac ttgagaatgt agaaataatt
     2221 agtcgtccac ttgtgtttgc aaagatttca ttaatagcac tgatgacggt tctttcactt
     2281 ggggtaaaac cttcatgttc ggcattcgtc gaatcactta aaaagagatc aacaccttca
     2341 ttaccaagtt cagcaatttt acctaaatcc atttggcgcc caattggtgt taggtcaatc
     2401 ttgaagtcgc cagtcccaac aacacggcca tgttcagtat caacacaaaa tccgtaacaa
     2461 tcagggatag agtgtgtcac ggcaaagaaa ctaacagtca tactaccaaa ttttactact
     2521 gtatcgccat cgtattcgac aatgttaacg ggtgtcttaa tacgcatcat ttgtaattta
     2581 tgacgaatga gcgctgctgc tagtgggggt gcataaataa caggtactgg tacaacctga
     2641 actagaaacg ggattgcccc aatatggtct tcgtgaccgt gagtaataaa aagtgctttt
     2701 attttgcgtg cattatcgcg taaataggca aaatcaggaa taacataatc aataccaggt
     2761 aaatcttgtt cggggaataa tacaccagcg tcaaaaagta ggatgttttt ttcatcttca
     2821 acaacataca tgtttttacc gacctcacct aaaccaccaa gggcataaat agaaaccggc
     2881 atttcaaatt ttgaattaat agccataatt atctcctaga attattaaat atatagatca
     2941 atatcttact tattatgcca aattaataat taaatgtaaa tttaaaagtc tctacaaaga
     3001 gagagcgtcc ggatatcgaa ctttgttttc ctcattaatc cggtcataaa ataagacacc
     3061 atggagatga tcaatctcat gttgcaatac gatagcagga taaccaaaag tatttatttc
     3121 gacttcttcg ttgtttaaga tatcaaaagc ttttactctt attttgtaat gacgatatac
     3181 atgaccagga tggtcttcgt ccactgataa acagccttca ccgctgcgta aatatgcacg
     3241 gcgcacagaa tcacgctcaa ctccagcggc taaaaaatcg gttaattggt ttatg
//
LOCUS       DAOPCD010000104         3285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_23254_length_3285_cov_3.263777, whole genome shotgun sequence.
ACCESSION   DAOPCD010000104 DAOPCD010000000
VERSION     DAOPCD010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23254_length_3285_cov_3.263777"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1841)
                     /locus_tag="PLQ55_03240"
     CDS             complement(<1..1841)
                     /locus_tag="PLQ55_03240"
                     /inference="COORDINATES: protein motif:HMM:NF004189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HPK28978.1"
                     /translation="MADKNITNSYTADSIQVLEGLDAVRKRPGMYIGSTNIVGFHQLV
                     WEVVNNSTDEAMSGYGDKITVTIHKNGSITIADEGRGIPTGINKQTGQPAVHLVFSTL
                     HAGGKFSESTYKTSAGLHGVGASVTNALSEWLDVTVYREGKVHHLRYENGGKLVTPLE
                     VVGNTNRTGTIVNFKPDPKIFGNLEFKWDTITSHLQESAFLLNKVAFIVKDERSGLSE
                     EFYYEKGLIEYVDIINRNKTALSKPIYFEDIYTNNDKLSQPMEIEVALQYCSDDYGET
                     VLSYVNNLRTHDGGTHETGFRTGLTRAVNDFATEHNLLRKGVKLDGNDIREGLTAIIS
                     VRIPESILQFESQTKGKLGTNEANWALNSFIYTKLTYYLQENHEFAVSLIKKCVDAQN
                     ARIAARKAKENLRKTKKVTNNDMILSGKLTPAQSKDYKKNELFIVEGNSAGGTAKSGR
                     DRMYQAVLPLRGKPLNTDTSTVDKMLKNEEFATLISTIGAGFGQNFDIEDAHYGKIII
                     MTDADYDGSHIQTLLLTFFYHYMKPLLKAGMIYIAVPPLYRVSKQVNKKLIFEYAWDD
                     QSLEAAKEKIGPGYKITRYKGLGEMNADQLWETTLNPETRTLIRVNI"
     gene            1946..2629
                     /gene="plsY"
                     /locus_tag="PLQ55_03245"
     CDS             1946..2629
                     /gene="plsY"
                     /locus_tag="PLQ55_03245"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPK28979.1"
                     /translation="MHPFIKFLIFLGFGLFGYLIGSINNAVIISNLFFKRDVRNYGSK
                     NAGGTNTGRVFGRKAGITVMVLDILKSVFVYWAITLVFHFTNVGLYVDHAATLYFAML
                     LVALGHCYPVYYKFKGGKAVSVVAGFILATNWVFTILGALIFFLILKLRKMVSYASIL
                     MAAIIVALTPILFIPVLGRISFYPLVVDNLYYYIPTLVGLCILLILKHRTNIERLQKG
                     TESKITWIK"
     gene            complement(2699..2911)
                     /locus_tag="PLQ55_03250"
     CDS             complement(2699..2911)
                     /locus_tag="PLQ55_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016340731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HPK28980.1"
                     /translation="MPIGAAIGLAVFALILGAVLGYLISRKVFEKQLKKNPPITEKQI
                     RTMLTMMGRKPSEKQVREIMRGMQQS"
     gene            complement(2911..>3285)
                     /locus_tag="PLQ55_03255"
     CDS             complement(2911..>3285)
                     /locus_tag="PLQ55_03255"
                     /inference="COORDINATES: protein motif:HMM:NF020845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPK28981.1"
                     /translation="RISISKHKMTYLYDSGDFLVFMNTETFEQVELSKESLTWELNFI
                     VPNSEVEMAGYEGEIFGITLAPKVALKITECAPGVKGNTVQNATKDAIVETGLRVSVP
                     LFIEEGEVIMVKTTDGSYDGRA"
BASE COUNT          902 a          652 c          553 g         1178 t
ORIGIN      
        1 ttaatattaa cgcgaattaa tgttcttgtt tcgggattaa gtgttgtttc ccataattga
       61 tcagcgttca tttcccctaa acctttataa cgcgtaattt tataaccggg accaattttt
      121 tctttcgctg cttctaaact ttggtcatcc cacgcgtatt caaagataag ttttttattc
      181 acttgtttac tcacacgata taaaggcggc acggcaatat aaatcatccc cgcttttaaa
      241 agtggtttca tatagtgata gaaaaaggtt aataataatg tttgaatatg tgaaccgtca
      301 taatcagcgt ccgtcataat aatgattttg ccgtaatggg catcttcaat atcaaaattt
      361 tggccaaagc cagcaccaat agtgctaatt agtgtcgcaa actcttcgtt ttttagcatt
      421 ttatcaacag tacttgtatc agtgttaagt ggtttaccac gaagtggtaa cactgcttga
      481 tacatccggt cacgtccgct tttagcagta ccaccagcac tattaccttc aacgataaag
      541 agttcatttt tcttataatc tttactttgc gctggtgtaa gtttaccgct taaaatcatg
      601 tcattattcg taactttttt cgttttgcgc aagttttctt ttgctttacg cgcggcgatt
      661 ctagcgtttt gcgcatcaac acatttttta attaaactaa cggcgaattc atgattttct
      721 tgtaagtagt aagtaagctt agtgtaaata aaactattta gtgcccagtt tgcttcattt
      781 gtgcctaact ttccttttgt ttggctttca aattgtaaaa tgctttctgg aatacgtaca
      841 ctgataattg ccgtaagtcc ttcacggatg tcgttaccat caagtttcac gcctttacgt
      901 agtaaattat gttcagttgc aaagtcatta acagcgcgtg ttaatcctgt tctaaaccct
      961 gtttcatgtg taccgccatc atgcgtgcgt aaattattaa cataggagag gacagtttca
     1021 ccataatcat cgctacaata ttggagcgct acttcaattt ccattggttg acttaattta
     1081 tcattattcg tataaatatc ttcaaagtaa attggtttac taagcgcggt tttattacga
     1141 ttaataatgt caacatactc aattaagcct ttttcataat aaaactcttc acttaaacca
     1201 cttctttcat ctttaacaat aaaagcgact ttattcagta agaaggcact ttcttgtaag
     1261 tgacttgtaa ttgtatccca tttaaattct aaattaccaa agattttagg gtcaggttta
     1321 aagttcacaa ttgtcccggt gcgattagtg ttccccacta cttctaaggg agtgactagt
     1381 ttaccgccat tttcataacg gagatgatgc acttttcctt cacgatatac agtcacatca
     1441 agccattcgc ttaacgcatt tgttacacta gcgccgacac cgtggagacc cgcacttgtt
     1501 ttataagttg attcactaaa tttaccacca gcgtgtaatg tggaaaatac gaggtgtacc
     1561 gctggttgtc cggtttgttt attaatgcct gtggggatcc cgcgtccttc gtcagcaatc
     1621 gtaattgagc cgtttttatg aattgtaaca gtgatcttat caccataacc actcattgct
     1681 tcgtctgtac tattattgac gacttcccag acgagttggt gaaaaccaac aatgtttgtt
     1741 gacccgatat acatcccagg tcttttgcgt accgcatcga gtccttctaa aacttgaatc
     1801 gaatcagcgg tatatgaatt tgtaatattc ttatctgcca tttgattttt ccttatcatt
     1861 tcacaatact ttgttattat aacaaaaaat tactttattt tataattgga ctattttaat
     1921 ttatactatt taagggaggt tacttatgca tccatttata aagttcttaa tattcttagg
     1981 attcggactc tttggctact taattggctc gattaataac gctgtcatta ttagtaatct
     2041 cttttttaaa cgtgatgtca gaaattatgg cagtaagaat gcgggcggga caaatactgg
     2101 tcgtgtcttc ggccgtaaag caggaatcac tgtaatggtg cttgatattt taaaaagtgt
     2161 ttttgtatat tgggcgataa ccctagtctt ccacttcact aatgtgggac tttatgttga
     2221 tcacgcagca acactttact ttgcaatgct tttagtcgcg cttggccact gttatccagt
     2281 gtattataaa tttaaaggcg gtaaagctgt gagtgtcgtc gctggtttta ttcttgccac
     2341 aaactgggta tttacgattt taggggcgct tattttcttt ttaattctta aattacgcaa
     2401 gatggtcagc tatgcatcga tcttaatggc cgctattatc gttgcactaa caccaatttt
     2461 atttatccca gtacttggtc gtattagttt ttatccgctc gtcgtcgata atctttatta
     2521 ttacatcccg acactagtcg gtttatgtat cttattaatt ttaaaacacc gcacaaatat
     2581 tgaacggctc caaaaaggca ctgaatcaaa aattacgtgg ataaagtaat tcttgccact
     2641 aataaataaa agctaggacg tgtgtcctag cgtttttatt tacatataat cttaataatt
     2701 aagattgttg cattccgcgc ataatttcgc ggacttgttt ttcacttggt ttacggccca
     2761 tcatcgttaa cattgtccga atttgttttt ccgtaatcgg tggatttttc tttaattgtt
     2821 tttcaaatac tttacgacta atgagataac ctaataccgc acctagaatt agcgcaaaga
     2881 ctgctaaacc tatcgctgct ccaattggca ttatgctctc ccatcgtaac taccgtcagt
     2941 tgttttgacc ataattactt ctccttcttc aataaataat gggacactga ctcttagtcc
     3001 tgtttccaca atcgcgtcct ttgtcgcgtt ttgtacggtg ttacctttaa cacctggcgc
     3061 acattcggta attttaaggg cgactttagg cgctaaagtt atgccaaaaa tttccccttc
     3121 ataaccggcc atttctactt cactattggg gacaataaag ttaagttccc aagttaatga
     3181 ttctttggat aattcaactt gttcgaaagt ttcagtattc ataaacacta agaaatctcc
     3241 actatcataa agataagtca ttttgtgttt gctaatacta atcct
//
LOCUS       DAOPCD010000105         3251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_23579_length_3251_cov_3.543805, whole genome shotgun sequence.
ACCESSION   DAOPCD010000105 DAOPCD010000000
VERSION     DAOPCD010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23579_length_3251_cov_3.543805"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PLQ55_03260"
     CDS             complement(<1..411)
                     /locus_tag="PLQ55_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HPK28982.1"
                     /translation="MENEKYKRLLQIISEEDLIKIQNKRIIIFGLGGVGGNALEALVR
                     SGFRRFTIVDNDVVDITNLNRQLIATTKTIGMPKVKAAKKRMLEIEPNCNINAVFEAV
                     TPQNVANFNLEFYDYIVDAIDSFEDKMALIVYAVK"
     gene            complement(371..1348)
                     /locus_tag="PLQ55_03265"
     CDS             complement(371..1348)
                     /locus_tag="PLQ55_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPK28983.1"
                     /translation="MKWVFLGLAILIFLVISLYFWTVMITFRMLFRRPKLPKKLVFPK
                     AFFGGVFDKHIPEIEANHKKNAKKACEIVTISSSAGKLKGRFYENENSNKIVIFIHGY
                     YSSGMRDIGYFGSLHKELNVSLLVIDQRANGESDGVYTSFGALERYDIREWIFYLNAR
                     YKGEKDIYLHGISMGAATSLLVTALAALPPSFKGVIADCGFSRTNGVIFFAGKRMLKI
                     KPKLTFWGLNLIAKLAGGFDLNKVNVSRELHKNMKIPILFIHGTADLFVPYNMSVKNY
                     KATSGPKKLVAFPDAGHCESYLSNKALYTEEMRKFIYGKRKVQKTTTNN"
     gene            complement(1416..2624)
                     /locus_tag="PLQ55_03270"
     CDS             complement(1416..2624)
                     /locus_tag="PLQ55_03270"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPK28984.1"
                     /translation="MKKSLVYFQSGGPTAIINTSMYGVIKEAKRQTEYIDGIYGSLHG
                     IEGLLNDNLIDLRQEDEAQIELLKQTPAAALGTTRYKLSGDINHEDYQKILNNVKKHN
                     IGYMLVNGGNDSMDTCYKLSTFFRKMDFDCKVIGIPKTIDNDLAFTDHCLGYPSAARY
                     VVQSIQDIALDNKVYPKSKFVIVEVMGRHAGWLTAAASVIQDPELKPDYIYLPEEKFD
                     LEEFLARAKEVYERKKRALFVVSEGIETQMPNLDKSVDAFGHAQLGGFAAKLAGIVEE
                     RLCYSTRPIEFSLLQRAGTLAITETDQKEAIKVSTVAVKRIVAGKTGQMVIIDRKPGP
                     KYKVSYKLKNLRGIANVEHKMTPELIHDVNNGGKLFGEYLFPLIDKPIKLQLENGVAK
                     YTKLKKVKVK"
     gene            complement(2669..>3251)
                     /locus_tag="PLQ55_03275"
     CDS             complement(2669..>3251)
                     /locus_tag="PLQ55_03275"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389207.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPK28985.1"
                     /translation="DIVTSTTHKTLRGPRGGIILTNSEEIIKKVNFTCFPGMQGGPLE
                     NIIGAKAQSFYEALQPEFKEYIKRVVENTKVASETLRALGAEASETETHLFLLNTKKS
                     YGITGKDAEALLETIKITTNKNMLPKDDERPNTTSGLRLGFAAVTTRGLTKVNAAETA
                     RIIHEFLSKKITKEEAMIRVNKIVDTLQAIDSL"
BASE COUNT          970 a          630 c          526 g         1125 t
ORIGIN      
        1 tttaacagca tacacgataa gcgccatctt atcttcaaaa ctatcaatag cgtcaacaat
       61 ataatcataa aattctaaat taaaattagc gacattttgc ggagtaaccg cttcaaaaac
      121 ggcattaata ttgcaatttg gctctatttc taacatacgc ttctttgccg cttttacttt
      181 tggcatacca atcgtttttg ttgtagcaat taattgtcga ttaagattag taatatcaac
      241 gacatcatta tcaacaattg taaagcgtct aaaaccggag cggacaagcg cttcaagcgc
      301 attaccacca acgccgccaa gtccaaaaat aatgatgcgt ttattttgga ttttaataag
      361 gtcttcttca ctaattattt gtagtagtct tttgtacttt tcgttttcca tatataaact
      421 ttctcatttc ttcggtgtag agtgctttat ttgataaata tgattcacaa tgacctgcat
      481 caggaaacgc cactagtttt ttgggaccac ttgttgcctt ataattttta actgacatat
      541 tataaggaac aaataaatca gcggtaccat gaataaataa aattggtatc ttcatgtttt
      601 tatgtaattc gcgacttaca ttaactttat taagatcaaa gccgcccgct aatttagcta
      661 ttaggttgag tccccaaaaa gtaagtttcg gtttaatctt aagcatgcgc ttaccggcaa
      721 aaaagattac accattagtg cgcgaaaatc cgcaatcagc gataacacct ttaaagctcg
      781 gtggtaatgc cgctagcgct gtaacgagta agcttgtcgc agcccccatt gaaattccgt
      841 gaagataaat atctttttcg cctttataac gagcatttaa ataaaagatc cattccctaa
      901 tatcataacg ctcaagcgcc ccaaatgatg tataaacacc atcactctcc ccgttagcgc
      961 gttggtcaat gacaagtagt gacacattta actctttatg taatgaacca aaataaccta
     1021 tatcacgcat tccagaagaa taataaccgt gaataaaaat aactatctta ttactattct
     1081 cgttttcata gaatcgtcct tttaatttac ccgcacttga actaatagtt actatttcac
     1141 atgccttttt agcatttttc ttatggttag cctctatttc cggaatatgt ttatcaaaaa
     1201 caccaccaaa aaaagctttt gggaacacta gtttcttagg aagttttggc cgacgaaaga
     1261 gcatccggaa tgtaatcatg acagtccaaa aatacagtga tattacgaga aatattaaga
     1321 ttgctagccc taaaaatacc catttcatac atttattata acttttaata aagaaaaagc
     1381 ggagtttata ctccgctcac tatttttttt actaattatt taactttaac ttttttaagt
     1441 ttagtgtatt tagcgacacc attttctaat tgaagtttga ttggtttatc gattagtggg
     1501 aataaatatt caccaaataa cttaccacca ttattaacat cgtgaatgag ttctggtgtc
     1561 attttatgtt caacattagc aataccgcgt aagtttttaa gtttataact tactttatat
     1621 tttggtcctg gtttacggtc gataataacc atttgtcctg ttttaccagc aacaatacgt
     1681 ttgaccgcaa cagttgaaac tttaattgct tctttttggt ctgtttcggt aattgctaaa
     1741 gtacctgctc tttgtaaaag actaaattca attgggcgcg ttgaatagca taagcgttct
     1801 tcaacgatac ctgctaattt ggcagcaaag ccaccaagtt gagcatgacc aaatgcgtcg
     1861 actgatttat caaggttagg catttgtgtt tcaatacctt cactaacgac aaataatgcc
     1921 cgttttttgc gttcataaac ttctttagcg cgcgctaaga attcttctaa gtcaaatttt
     1981 tcttcaggta aataaatata gtctggttta agttctggat cttggataac acttgcggcc
     2041 gcagttaacc atccagcgtg gcgccccatc acttcgacga taacgaattt tgattttgga
     2101 tacactttat tatctaaagc gatgtcttga attgattgga caacataacg ggctgcactt
     2161 ggataaccta agcagtgatc agtaaacgct aagtcgttgt caattgtctt tggaataccg
     2221 attactttac aatcgaagtc cattttacgg aagaaagttg ataatttgta acaagtatcc
     2281 attgaatcgt taccaccatt aactaacatg taaccaatgt tatgtttttt aacgttgttt
     2341 aatatttttt ggtaatcttc atgattaata tcaccagaaa gtttatagcg cgttgtccct
     2401 aacgccgcag caggggtttg ttttaataat tcaatttgcg cttcatcttc ttgtcgtaaa
     2461 tcaataagat tgtcgtttaa taaaccttca ataccgtgta aactaccata aattccatcg
     2521 atatattcgg tttgccgttt ggcttctttg atgacaccat acattgaggt gttaatgatt
     2581 gcggttggtc cgcctgattg gaaatacact aatgattttt tcatattttt actccttttc
     2641 tcacccgaga ttataccatg agtgactatt aaagactatc gatagcctgt aaagtatcaa
     2701 caattttatt aactctaatc atcgcttctt ctttcgtaat cttcttactt aagaattcat
     2761 gaatgattct tgcggtttcg gcagcattaa ccttagttaa tccgcgtgtt gttaccgccg
     2821 caaagcctag gcgaagacca gatgtcgtat ttggtctttc atcatcctta ggtaacatat
     2881 ttttattcgt tgtaatttta attgtttcaa gtagtgcttc agcgtctttt ccagtaatac
     2941 catacgactt tttagtgtta agtaaaaaga ggtgtgtttc tgtttcactc gcttcggcac
     3001 caagggcacg aagtgtttca ctagcgactt tagtgttttc tacaacacgc ttaatatatt
     3061 ctttaaattc gggttgcagc gcttcataaa atgattgcgc ctttgcgcca ataatatttt
     3121 caagtggtcc gccttgcata ccagggaaac aagtaaaatt aactttttta ataatttctt
     3181 cactattagt aaggataata ccaccgcgcg gtcctcttaa tgttttatgg gttgtacttg
     3241 ttactatatc a
//
LOCUS       DAOPCD010000106         3250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_23594_length_3250_cov_3.804695, whole genome shotgun sequence.
ACCESSION   DAOPCD010000106 DAOPCD010000000
VERSION     DAOPCD010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23594_length_3250_cov_3.804695"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..667)
                     /locus_tag="PLQ55_03280"
     CDS             complement(62..667)
                     /locus_tag="PLQ55_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HPK28986.1"
                     /translation="MDSKNLYDFLIKVQSIAKIGLLYTTDPYAKKNYIEIGQMTLSLL
                     EDLQAVNLERNNYFKRDIYPTPNISVRAIIFNKEGQLLMVQEKEDGGFSLPGGWADLY
                     DSPTEAILKETTEEAGAKIKIMGIVAFLHRTPFKTPTSVPEYVLVFKAEFVEFIGEHD
                     HEILTVKWVDPYNLPPLSHKVTKHEVERMINAALHDKTIFD"
     gene            763..1527
                     /gene="pgeF"
                     /locus_tag="PLQ55_03285"
     CDS             763..1527
                     /gene="pgeF"
                     /locus_tag="PLQ55_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HPK28987.1"
                     /translation="MYKWNDFKKITAVTSKRTGGFSTGDFSSLNLALHVGDDKETVFK
                     NRECFFGKKEVMMAPKNSVFVAQNHSTIVKKVTFKDAGRGFYDFNDGIKADALYTDEP
                     GLNLAIYHADCVPVFIYVPTHNIVGIIHAGEQGSVGNITGILVRKLFLEGVKPEEVYA
                     HIGPSASMGHRVITKERAFELAALGGDIIKAIKATEPQYFIDLPLLNTLQLRTLGVPL
                     SNITYSNICTYENNDEYYSYARNKVTGRNISFIRLN"
     gene            complement(1528..2004)
                     /gene="coaD"
                     /locus_tag="PLQ55_03290"
     CDS             complement(1528..2004)
                     /gene="coaD"
                     /locus_tag="PLQ55_03290"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007086244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPK28988.1"
                     /translation="MKLIAIYPGSFDPITNGHIDILNRALKIFDNVIVLVADNPAKKP
                     TFSVEERKAMIRESINGNPNVKVDSTTGLTIHYAEKVGAYALIRGLRAAIDFEYEFQI
                     NSANSFINSGVESVFLMAKQEFTFISSSTIKEMVSGGVDVSSLVPKPVLKRLEKLK"
     gene            complement(2001..2543)
                     /gene="rsmD"
                     /locus_tag="PLQ55_03295"
     CDS             complement(2001..2543)
                     /gene="rsmD"
                     /locus_tag="PLQ55_03295"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPK28989.1"
                     /translation="MLAKGRVIAGKYRHRHLIALFDEKTRTTKDRVKEAIFSAVGDYI
                     NGKTVLDLFAGTGALGIEAISRGAAHGIFCENDSKTYDVLTGNLQMIEEPYNLYFEDY
                     KSALAKIKPNSVSIVIIDPPYRYDIDEIINNVFKAKILTNSYTLVLETDKEYAGVINN
                     AKIKKYKYGLTHVTIIRGEL"
     gene            complement(2522..2896)
                     /locus_tag="PLQ55_03300"
     CDS             complement(2522..2896)
                     /locus_tag="PLQ55_03300"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HPK28990.1"
                     /translation="MNQKLYELALDLHQNLEEHPSVLLLKEYEKAMLKDSTVKALIEQ
                     YDALQEEINSLLESCDLENKAIKAKCSTLYKLKYVLDNEPLVKRYNKQYNLVNKMYKN
                     ITEELTNAVGIKKEIKCLLKDE"
     gene            2989..>3250
                     /locus_tag="PLQ55_03305"
     CDS             2989..>3250
                     /locus_tag="PLQ55_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPK28991.1"
                     /translation="MLKIFKDSDPLLRKRAEQVSLPLSDSDRELALAMLEHLKESQDP
                     LFAEEHNLRPGVGLAAPQVGESKQIIVVHIPRGKTERLTYVLA"
BASE COUNT         1005 a          590 c          530 g         1125 t
ORIGIN      
        1 atctccttta attttatttt ttattatagc atttatgcgc tccacgtggt acgagtatat
       61 gctaatcaaa aatagttttg tcatgaagcg cggcattgat cattcgttca acttcatgtt
      121 ttgtaacttt atgtgaaagt ggcggcaagt tataaggatc aacccatttt acggtaagta
      181 tttcatggtc atgttcccca ataaactcaa caaactcagc tttaaacact aaaacatatt
      241 ccggcacaga ggtaggagtt ttaaaaggtg tgcgatgcaa aaaggccaca atccccataa
      301 ttttaatttt tgctccagct tcttcagttg tttcttttaa gatggcttca gttgggctat
      361 catataaatc tgcccatcct ccaggcaacg aaaaaccccc gtcttccttt tcttgaacca
      421 ttaataattg gccttcttta ttaaaaatga tagcgcgaac gctaatattt ggtgttggat
      481 aaatatctct tttgaaataa ttatttcgct ctaaattcac tgcttgcaga tcttcaagta
      541 atgaaagcgt catttgtcca atttcaatat aatttttctt tgcgtacggg tcggttgtat
      601 ataataatcc aatcttagcg attgattgta ctttaataag aaaatcataa aggtttttac
      661 tatccataat aaatttagta taacatacta gtttaaagtg atggcttact atgaataata
      721 atttaactac tttttatgta taataagaat aggaggcgcc ttatgtataa atggaatgat
      781 tttaaaaaga ttacagctgt tacttcaaaa cggactggcg ggttttcaac cggagatttt
      841 tcttcattaa atctagcact acacgttggt gatgataaag aaacagtttt taaaaataga
      901 gaatgctttt tcggcaaaaa agaagtgatg atggcaccta aaaatagtgt gtttgttgct
      961 caaaatcata gtacgatcgt taaaaaggta acttttaaag atgctggacg cggtttttat
     1021 gattttaatg atggaattaa agctgatgct ctctatactg atgaacctgg acttaatctt
     1081 gctatttatc acgctgattg tgtaccagtt tttatttatg tgccaacaca taacatcgta
     1141 gggattattc acgctggtga acaaggaagt gtcggcaata ttactggtat tctcgttcgt
     1201 aaattattcc ttgaaggtgt taaacctgaa gaagtttatg cacacattgg accaagcgca
     1261 agcatgggtc atcgtgtaat tacaaaagag cgtgcctttg aacttgctgc gttaggtggt
     1321 gatatcatca aggcaataaa ggcaactgaa cctcaatatt tcattgatct accactactt
     1381 aataccttgc agcttcgcac gcttggtgta ccacttagta atattactta cagcaatatt
     1441 tgtacatatg aaaataacga cgagtactac tcctatgcaa ggaataaagt aacaggtcgt
     1501 aatatttctt ttattcgttt aaactaatta ttttaatttt tctaatcgct ttaaaactgg
     1561 tttaggtacg aggctactta catcaacgcc tccgctaacc atctctttaa ttgtgctact
     1621 agaaataaat gtaaattctt gtttagccat taaaaagact gattccacgc cagaattaat
     1681 aaaactattt gcactattga tttggaactc atattcaaag tctatggcgg cgcgtaaccc
     1741 acgaatcagg gcgtatgccc ctactttttc tgcataatgt atagttaaac cagttgttga
     1801 atcaacttta acatttggat taccatttat tgattctcta atcatcgctt ttcgttcttc
     1861 caccgaaaat gttggctttt tagcgggatt gtctgcaact agaacaatga cattatcaaa
     1921 tatttttaac gctcgattca aaatatcaat atgcccattt gtaatcggat caaaactccc
     1981 cggatatata gctattaatt tcataattca cctctaatta tggtaacgtg tgtcaaccca
     2041 tatttatact tttttatttt agcattattt atcactcctg catattcttt atctgtttct
     2101 aatacaagag tgtaactatt tgttaatatt ttagctttga aaacattatt aataatttcg
     2161 tcaatgtcat aacggtaggg tgggtcaata ataacaatag aaacggaatt aggcttaatc
     2221 ttcgctaaag cactcttata atcttcaaaa tataaattat aaggctcttc aatcatttgt
     2281 aagtttccag ttaatacatc atatgtttta ctatcatttt cacagaaaat accatgagca
     2341 gcgccacggg agattgcttc aataccaagt gccccagtac cggcaaataa atcaagaact
     2401 gtcttgccgt ttatataatc acccactgcc gaaaaaattg cttcttttac acggtctttt
     2461 gttgttcgtg ttttctcgtc aaaaagggca ataaggtggc gatgtcgata ctttccagca
     2521 attactcgtc ctttagcaag catttaatct ccttttttat tcccactgca tttgtcagtt
     2581 cttctgtgat atttttatac attttattaa caagattata ttgcttgttg tagcgtttta
     2641 ccagtggttc attatctaaa acatacttta atttataaag agtactgcat tttgctttaa
     2701 tcgctttatt ttctaaatca cacgactcaa gcaaactatt aatctcttct tgcaaggcgt
     2761 catattgctc aatcagtgcc ttaactgtgc tatcttttag cattgctttt tcatattctt
     2821 tcaaaagtaa aacacttgga tgctcttcaa gattctgatg taaatcaagc gctaattcat
     2881 atagtttttg gttcataaac taattataca agaaatgatg aattatcata tagcgttaat
     2941 actcacttgt taacacagat gtgttaaaat atttccggag ttagaagaat gctgaaaata
     3001 tttaaagata gtgatccctt attacgcaaa agagccgaac aagtttcgct cccactttca
     3061 gattctgatc gagaactcgc ccttgcaatg cttgagcatt taaaggaaag ccaagacccg
     3121 ctttttgctg aggaacataa cttacgacca ggagtaggtt tagctgctcc acaagttggg
     3181 gagagtaaac aaattattgt cgttcatatc ccccgtggta aaactgaaag attaacatat
     3241 gttctcgcaa
//
LOCUS       DAOPCD010000107         3244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_23639_length_3244_cov_3.068987, whole genome shotgun sequence.
ACCESSION   DAOPCD010000107 DAOPCD010000000
VERSION     DAOPCD010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23639_length_3244_cov_3.068987"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PLQ55_03310"
     CDS             <1..421
                     /locus_tag="PLQ55_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28992.1"
                     /translation="MTDKQATEVAAASLEIVESIPLNVNNVTLPTTLEGYEGVTVEWT
                     SSNAALNTETGAVTHPVSTLDLTLTATVKKGTDQTVRSWTVTVFGTNLESLSFLHTLD
                     LEDADPSGSYGCSNTKPKYTVEATNDMVDLGDPVTKW"
     gene            470..802
                     /locus_tag="PLQ55_03315"
     CDS             470..802
                     /locus_tag="PLQ55_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28993.1"
                     /translation="MYAIRTQSNNDQARSGRIELQDNVYDFNMLEFDAAIYGANRKGM
                     VLMFEVSTDDGVTWTQLEREYTINHFDLVKYRVRTGTTGNVRVAIVLKAGTGQRINID
                     NIRLIKEA"
     gene            807..2324
                     /locus_tag="PLQ55_03320"
     CDS             807..2324
                     /locus_tag="PLQ55_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPK28994.1"
                     /translation="MRRLATIGLLVILALTGCGKRTSSEVSLTTSAPSLSDEPSIATP
                     LSIQVFKNDIRAYDVTVDVSHTINLEARVLPLDAPQEVVWSVVNSELATVSATGAVTG
                     VAPGETMINVHALNYPDVKTSLFLTVNKVAEQTGVGSGTSADDPLFKGYEGDGPLEVY
                     FLETYKIYADSLYIKKGNIDIVIDGGWKCDGELNRDFLNEKVTDGRIDMLIASHGDGD
                     HTQGIPYLVRDIPHISSFLDYGRAGGESSGYKQLIRTFGENEGSPYYTALDSVNKVNG
                     ATNRYYFTSEFYVDVLNTGAYSDTSSGNANSLALMFCYKDFKFFTAGDLTTGSETSLL
                     RNETLPQVSLYKASHHGSHGSNSQALMDTLNPYTVAIAATRAGIYNQDPAPPSPTNTY
                     NLDGTKGHPAAAAIERIYKIPRIFLNLNVFWNAPNGTMKFTTYGGANDLVMNGSTPLR
                     HYYDLTLTNGEPVWNEELQDFENKVTGEENTKFHETKIFTFRGYTEYLPAWVTAA"
     gene            complement(2396..>3244)
                     /locus_tag="PLQ55_03325"
     CDS             complement(2396..>3244)
                     /locus_tag="PLQ55_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HPK28995.1"
                     /translation="DSLYDSKFYHDTINDIYLGFVCKSAENDYFGYLKKMLLTLHNLY
                     MINHDNLPVHGAMVEITLKSNIKKRVVIVGDSGAGKSETLEALRQIASDEIKDIKVVF
                     DDMGTLSTNEDGKVLASGTETGAFVRLDDLEQGYAYEVMDRALFLNPSQVNARVVIPL
                     SRYSFILEKYPIDLFLYANNYEDKDGIKVFDKPEEALAVFRKGMRRAKGTTSEVGMTA
                     SYFANPFGPMQYRAKTDRLLNKYFKALSDNKVILGELYTKLALPEEEIRGPANAALAL
                     FKLLQG"
BASE COUNT         1012 a          637 c          633 g          962 t
ORIGIN      
        1 gatgactgat aagcaagcca cagaagtcgc ggccgcaagt ctagaaatcg ttgaaagcat
       61 tccgcttaat gttaataacg tcacacttcc aactacttta gaaggctacg aaggtgtaac
      121 tgttgaatgg acttcaagta acgcagcact taacacggaa acaggtgctg ttacgcatcc
      181 agtttcaaca cttgacctca cattaacggc aacagtcaaa aaaggaactg accagacagt
      241 tcgctcttgg actgtaaccg tctttggtac aaacttagaa agcctttcat tcttacatac
      301 attagactta gaagacgctg atcctagcgg tagttatggt tgtagtaata caaaaccaaa
      361 atatactgta gaagccacaa atgacatggt tgatttaggc gaccctgtta ctaaatggta
      421 acttaaaaac gccttaattg gcggcgataa taatgataag cgaactggaa tgtacgctat
      481 tcgtacccaa agtaataatg accaagctcg ttctggacgg attgagttac aagataatgt
      541 ctatgacttc aatatgcttg aatttgacgc tgcaatttat ggtgctaatc gtaaaggcat
      601 ggtcttgatg tttgaagttt caacggatga tggtgtcact tggacgcagc ttgaacgcga
      661 atacacaatc aatcactttg atttagttaa gtatcgtgta cgtactggta caacaggtaa
      721 tgttcgtgtt gccatcgtct taaaagctgg tactggtcaa cgcatcaaca ttgataatat
      781 tcgcttaatc aaagaggctt aagcttatga gacgtttagc cacaattggt ttattagtaa
      841 tcttagcgct taccgggtgc ggtaagcgca cctcttcgga ggtgtcactc acaacaagtg
      901 caccttcatt aagtgatgag ccttcaatag ccacaccttt aagcattcaa gtttttaaaa
      961 atgatattag agcatatgat gtcacagttg atgttagtca cacgattaat ttggaggcta
     1021 gagtattacc gctagatgcc ccgcaagaag ttgtgtggag tgtcgttaat agtgaactag
     1081 caaccgtgag cgctacaggt gcggtaactg gtgtagcgcc tggcgaaaca atgattaatg
     1141 tgcacgcgct taattatcct gatgttaaaa ctagtttgtt tttaactgtt aataaagttg
     1201 cagagcaaac aggtgtcgga agtggtacaa gcgctgatga tccacttttt aagggctatg
     1261 aaggcgacgg cccacttgaa gtttatttcc ttgaaacata taaaatatac gctgattcgc
     1321 tttatattaa aaaaggcaac attgatattg tgattgatgg tggctggaag tgtgacggtg
     1381 aacttaatcg tgacttctta aatgaaaagg taactgacgg aaggattgat atgcttattg
     1441 ctagtcatgg ggacggtgac catacacaag gtattcctta tcttgtgcgc gacataccgc
     1501 atattagtag cttccttgat tacggcagag ctggcggcga aagcagtggc tataaacagt
     1561 taatccgcac ttttggtgaa aacgaaggtt cgccatacta cactgctcta gattccgtga
     1621 ataaagtaaa cggtgcgact aatcgctatt acttcaccag tgaattttat gttgatgttt
     1681 taaataccgg agcatatagt gacactagca gcggtaacgc taatagtctc gcactaatgt
     1741 tctgttataa agactttaaa ttctttactg ctggtgactt aaccactggt agtgaaacta
     1801 gtcttttacg caatgaaaca ttaccacaag taagtttata caaagctagt caccacggga
     1861 gtcatggtag taacagtcaa gccttaatgg atacattaaa cccctatact gttgcgattg
     1921 ccgcgacaag agccggtata tacaatcaag atcctgctcc accaagtcct actaatacat
     1981 ataatttaga tggcactaag ggtcaccccg cagcagcagc gattgaacgc atttataaaa
     2041 ttccgcgcat ttttttaaac ttaaatgtct tttggaatgc tcctaacgga acaatgaaat
     2101 ttacaacata tggcggcgct aatgacttag tgatgaatgg tagtacccca cttcgccatt
     2161 attatgactt aacactcaca aacggtgaac ctgtatggaa cgaagaactc caagactttg
     2221 aaaataaagt aacaggtgaa gaaaacacta agttccatga aactaaaatc tttacattta
     2281 gaggttatac cgagtactta ccagcgtggg ttaccgctgc ttaaataatt aaaatacagc
     2341 aaaaaagtca ggagcgattc ctgacttttc tttatgaata gttaaaacga tcttactacc
     2401 cttgtagtaa tttaaatagc gctagcgcag cgttcgcggg tcctctaatt tcctcttcag
     2461 gaagcgctaa tttagtgtag agttccccta aaattacttt attgtcacta agcgctttaa
     2521 agtatttatt aagtaatcga tctgtcttag cgcgatattg cattggacca aacggattag
     2581 cgaagtaaga agcagtcatt ccgacttcac tcgtagtgcc ttttgccctt cgcattcctt
     2641 tacgaaaaac agcgagtgct tcttcaggtt tatcaaatac tttaatcccg tctttatctt
     2701 cataattatt cgcatataag aagagatcaa tcggatactt ttctaaaata aatgagtatc
     2761 tacttaaagg aataacgact ctagcgttaa cttgcgaagg attaagaaat agtgctcgat
     2821 ccattacttc ataagcatag ccttgttcta agtcatctag tctaacaaaa gcacctgttt
     2881 ctgtcccact tgcaagtact ttaccatctt cgttagtgct tagtgtcccc atatcatcaa
     2941 acacgacttt aatgtcttta atttcgtcac ttgcaatttg ccgtaatgct tctaaagttt
     3001 cacttttccc agcaccacta tcaccaacaa taactactct ttttttaatg tttgatttta
     3061 aagtaatctc taccattgca ccatgtactg gtaaattatc atgattaatc atataaagat
     3121 tatgaagtgt aagtagcatc tttttaagat aaccaaaata atcattttca gcacttttac
     3181 agacaaaacc aaggtaaata tcattaatag tgtcatgata gaatttagag tcatagagcg
     3241 aatc
//
LOCUS       DAOPCD010000108         3231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_23764_length_3231_cov_3.579345, whole genome shotgun sequence.
ACCESSION   DAOPCD010000108 DAOPCD010000000
VERSION     DAOPCD010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23764_length_3231_cov_3.579345"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1559
                     /locus_tag="PLQ55_03330"
     CDS             198..1559
                     /locus_tag="PLQ55_03330"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK28996.1"
                     /translation="MKVKLVAQLRFLLSLFELLFGELQEIPGSTLGDILVLLNTIVLS
                     FLSVLYFYQVLLSIFSVVVRAKKYPETDVFPTYTFVTCARNEETVIAQLITTIRALDY
                     PQEKINIHVVADNCTDNTAIIAAQLKAKVYTRNDLTKIGKSYALEYYFNEQKKVVPAE
                     DFAGYIVIDTDNVLDAQFLKEVNKVYQATNAKVVATYRASTNAGDSLWAFGTGYSFLR
                     ECCLMHKGREVLGLSSYVSGTGFFVSYEKMQEVEGWNHHLLIEDIEFSAAHVMEKGRT
                     HYAHNAIFYDEQAVNFKSSWHQRMRWVKGLFQVSKVYTWSLFKSIFARKRTLKERFSS
                     YEAFLFSTPFPAYIIWWFILYGIGAAINLAITQDINYFIQTYLFTLFDFLLGFYLFTT
                     LMSLLISVSNWKRIKMHPLKKIMLPFVAFFFLLSYVPMLLIAPFVKVTWRPVKHYGLK
                     KPL"
     gene            complement(1554..2531)
                     /gene="fni"
                     /locus_tag="PLQ55_03335"
     CDS             complement(1554..2531)
                     /gene="fni"
                     /locus_tag="PLQ55_03335"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HPK28997.1"
                     /translation="MSRRRKDEHIAIAKMAKIRKNDFSHIRFIHHSIPSLSLKDIDLS
                     TSFLGHKFNYPFYINAMTGGAKKGDKINAKLAILAQHFGLPLFVGSQSLAFKNKESRL
                     AFETLRFNFPDLFIVANINPNFTAEMASDAVEILGANALAIHVNSVQELVMGEGDRDF
                     TKWKDNIANILKTVKVPVIVKEVGYGMHEPTLLLLQELGVKHIDISGMGGINFSLIEL
                     KRQKKKDSPLLDFGISTVDALLLARNYPSFEVLASGGINNASSIIKSLAMGAKAAGMA
                     KYFLDLCNLPVPEMIKKVDELIIDLKKILVLLNVKSVHELNLDHLMFDL"
     gene            complement(2524..>3231)
                     /locus_tag="PLQ55_03340"
     CDS             complement(2524..>3231)
                     /locus_tag="PLQ55_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK28998.1"
                     /translation="QFEYTIENNLISDEQVKFGLGSSAASVILTLKLLNRLYRTNLSP
                     LVLFKMAVLIQRELNALTSGGDLACTIFDMPLYYVRYDLNWLLNEKRTFELLEVAWPL
                     LEIKLLTKLPTFLVGWTKETITPDTNGTIDSAFFKKANKLVHHYLVTYDDIYIKQYQN
                     LLDKLGLTRQGMITPRLEALVKTARSLGVAAKISGAGYGDCGIAQINDQSVVEPLTNL
                     WENEGIIVLDIWRKDNE"
BASE COUNT         1040 a          576 c          540 g         1075 t
ORIGIN      
        1 cagtgccaaa gccaagagat gttaaaaaaa atacagaaat gtataaaaga aagcatcgcc
       61 gagaaaacgc acgaagtaag ttagtcttaa gtattcatac gtgcgctctt tagcgagtct
      121 tctcattgct ttcataatat caactaaatg ttatcatttt tcgatagtat ttaccccaaa
      181 atagatataa taattttatg aaagtaaaat tagttgcgca gttgcgtttt cttcttagcc
      241 ttttcgaact attatttggg gaattacaag aaattccggg ctcaacactt ggagatatat
      301 tagttttatt aaacacaatt gtcttatcct ttcttagtgt tttatatttc tatcaagtgc
      361 tactaagtat cttttctgtt gttgttcgcg ccaaaaaata tccagaaacc gatgtatttc
      421 ctacatatac attcgtgaca tgtgcgcgga atgaagaaac agtgattgcg caacttatca
      481 caacaattag agcgcttgat tatccccaag aaaaaataaa catccatgtt gtcgccgata
      541 attgtactga taataccgca attattgctg cgcagttaaa agcgaaagtt tatacacgga
      601 acgatttaac aaaaattggt aaaagctatg cacttgaata ttatttcaat gagcaaaaaa
      661 aagtagttcc cgcagaagac tttgccggct acattgtgat tgataccgat aatgtgcttg
      721 atgcgcaatt tctaaaagaa gtgaataaag tctatcaagc aacaaacgct aaagttgtcg
      781 cgacttatcg cgctagtaca aacgctggtg attcactttg ggcatttgga acggggtact
      841 cttttttacg tgaatgttgc ttaatgcata aaggtcgtga ggtgttaggt cttagttctt
      901 atgtatcagg cacaggtttt tttgttagtt acgaaaaaat gcaagaagtt gaaggctgga
      961 accatcattt attaattgaa gacattgagt tttcagcagc gcatgtcatg gaaaaaggcc
     1021 ggactcatta tgcacataac gcgattttct atgacgaaca agctgttaac tttaaatcaa
     1081 gttggcatca acggatgcgt tgggtaaaag ggttatttca agtttcaaaa gtttacactt
     1141 ggtcattatt taagtccatt tttgcccgca aaaggacact aaaagagcgt ttttcttctt
     1201 atgaagcgtt tcttttttcg actccgtttc cagcttacat catttggtgg tttatattat
     1261 atgggatagg tgctgctatt aacttagcga tcactcaaga cattaattat tttatacaaa
     1321 cttatctttt tacattattt gatttcttat tagggtttta tctttttact acactaatgt
     1381 cgttattaat tagtgtttca aattggaaac ggattaaaat gcatccactt aaaaaaataa
     1441 tgctaccgtt tgtagcattc tttttcttgc tttcatatgt accaatgtta ctaattgcgc
     1501 cattcgtgaa agttacctgg cgtccagtta agcattatgg tttaaagaaa ccattataaa
     1561 tcaaacatca agtgatcaag atttaattca tgaacactct taacatttaa taacactaaa
     1621 attttcttta agtcgataat taattcatca acttttttaa tcatttcagg aactggtaag
     1681 ttacataaat cgaggaaata tttagccata cctgccgctt tagcacccat cgctagtgat
     1741 ttaataatcg aactagcgtt attaataccg ccacttgcaa gcacttcaaa gcttggataa
     1801 ttacgggcaa ggagtaatgc atcaacagtg gaaataccaa aatcaagaag tggtgaatct
     1861 tttttctttt gtctttttaa ttcaatcaag gagaaattaa taccacccat tccgcttata
     1921 tcaatatgct taacaccaag ttcttggagg agtaagagag tcggttcatg cattccgtat
     1981 cctacttcct taacaattac cggtacttta acggttttta aaatgttagc aatattatct
     2041 ttccattttg taaaatcacg atccccttca cccatcacta attcttggac actattaaca
     2101 tgaatcgcta acgcattagc gcccaagatt tccactgcat cactagccat ttctgcagtg
     2161 aaattggggt taatatttgc gacaataaat agatcaggga agttaaatct aagtgtttca
     2221 aatgcaagtc gtgattcttt gtttttaaac gctaaacttt gtgagccaac aaacaaagga
     2281 aggccaaaat gctgggctaa tatcgccaat ttagcattta ttttatcacc ttttttagcg
     2341 ccgcctgtca ttgcattaat gtaaaaagga taattaaatt tatgtcctaa aaatgaagtc
     2401 gaaagatcga tatcttttaa tgataaagaa ggaatactat gatggataaa acgaatgtga
     2461 ctaaaatcat tttttcttat cttagccatt tttgcaatcg caatatgttc atctttacgg
     2521 cgtctactca ttatctttcc tccaaatatc aagtacgatg ataccttcat tttcccataa
     2581 atttgttaat ggttcaacaa cactctggtc attaatttgg gcaatcccgc aatcaccata
     2641 tcctgcccct gatattttag cggccactcc taaagagcgg gcagttttca ctaatgcttc
     2701 taaacgcgga gtaatcattc cttgtcgcgt taatcctaat ttatcaagta ggttttgata
     2761 ttgttttata taaatatcat cgtaagtaac taaataatgg tgaacaagct tatttgcttt
     2821 tttaaagaaa gcggaatcaa tagtgccgtt agtgtctggt gttattgttt cttttgtcca
     2881 acccacaagg aaggtaggaa gttttgttaa taatttaatt tctaaaagcg gccacgcaac
     2941 ttcaagtaat tcaaatgttc gtttttcatt aagtaaccaa tttaaatcat aacgaacata
     3001 ataaagcggc atatcaaaaa tagtacacgc taagtcgcca ccagaagtaa gcgcatttag
     3061 ttcacgctgg attaagactg ccattttaaa aagcacaagt ggtgacaaat tagtgcgata
     3121 taaacgattt aataacttaa gtgttaggat aacactcgct gcgcttgatc ctaaaccaaa
     3181 cttaacttgt tcatcactaa ttaaattatt ttcaatagta tattcaaatt g
//
LOCUS       DAOPCD010000109         3157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_24432_length_3157_cov_3.886847, whole genome shotgun sequence.
ACCESSION   DAOPCD010000109 DAOPCD010000000
VERSION     DAOPCD010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24432_length_3157_cov_3.886847"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1570
                     /locus_tag="PLQ55_03345"
     CDS             <1..1570
                     /locus_tag="PLQ55_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPK28999.1"
                     /translation="RLLLQDGTQQEVSVESLKVGDKVLVLNGDQIPTDGTIVSGSAAI
                     NEAMITGESMPKEKHEGDEVFGGSINGNSTFTMVVTKNSDETVLAKILRVVSETQTNL
                     TKTATFIKKFEPIYVTIVMLIAPLFYLFGYYVLGWNEVISANGMTLALKRTIILFIGA
                     SPCALAVSDIPASLSALSLLARRGVLFKGASSLALFADVEAVAFDKTGTLTEGKPSVT
                     DVNIPPGVSAEEAEEGKVFLVSMEKKSNHPLATAIVSHFTPIKTVDLEVEHKIGSGLR
                     AMYDGNEYLVGKPALFNLPERCANRAKELAVQGKTVVFIAKNGKPILLIALLDMIKCC
                     AFDGLEYFRKQEINTVMITGDNELTAKAIGKELGITRIYANVLPEQKLDIVKELKKEY
                     GGVAMVGDGINDAPALVEATIGIAMGDGTDVAIDVADGVLVNNDFQKLSLMHAISRKL
                     KRIVIQNIVLALSVVLLLIALNFVVTLPMGLTVAIHEGSTILVILNGLRLLNFNEKHA
                     IKKPKRSKFNKKIA"
     gene            1695..2711
                     /gene="hrcA"
                     /locus_tag="PLQ55_03350"
     CDS             1695..2711
                     /gene="hrcA"
                     /locus_tag="PLQ55_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HPK29000.1"
                     /translation="MRYRRRELILKYTVELFIKTAQPVGSKSLIDNYNLNYSSATVRA
                     EMNALEGMGFLEKPHASAGRVPSKKGYEFYVANLREDEVNDNFRHHIATLVEEKTKSV
                     EQVINQTCEILSQMTNLVSVALGPEKKGETLQSIQVVALDKNMGTAIFVTNRGYVEHN
                     TFIVPPSTSIQEISKVANIFNERLVGTRISEVSAKMELLQPLLTDYIVEQEVLANAFT
                     DAFIRYARDRLSLYGKESLLEQPEFINDSEKLRKLISILDDPEKIRELITGNGDLSVN
                     IGGKDEDLTFISANVHVPGDQVGQIVLVGPKRMDYSKVLWALEYVTDELEKYFEIERE
                     INDE"
     gene            2701..>3157
                     /locus_tag="PLQ55_03355"
     CDS             2701..>3157
                     /locus_tag="PLQ55_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPK29001.1"
                     /translation="MMSDEKKHVEKDVAPKKRHLAKIQKLEAEIEKLKAESAEWHNKY
                     MRVLADMDNARKQNAKDQQYYIKYRAVPFIEKLLPALDIFNHVIREEQEDPVLNNYLT
                     GFRFVYGQLLDALTSEGLEEVVVNVGDKFDENSMHAVEVKYSEEVEPNTV"
BASE COUNT         1068 a          524 c          695 g          870 t
ORIGIN      
        1 taggctttta cttcaagacg gaacacaaca agaagtaagt gttgagtcac taaaagttgg
       61 tgacaaagta cttgtgttaa atggtgatca aatcccgaca gatggcacga tagttagtgg
      121 tagtgccgca attaatgaag cgatgattac gggtgaaagt atgccaaaag agaagcatga
      181 aggtgatgaa gtctttggtg gtagtattaa tggcaacagt acttttacga tggtagtgac
      241 caaaaatagt gatgaaacgg tattagcgaa aatcttaagg gttgtgagtg aaacgcaaac
      301 taatttaact aaaactgcga catttattaa gaaatttgaa ccaatttatg taactattgt
      361 gatgttaatt gctccactat tttatttatt tggctattat gttttagggt ggaatgaagt
      421 tattagcgct aacgggatga cgcttgcgct taaacgcaca attattcttt ttatcggtgc
      481 ttcgccgtgt gccctagctg ttagtgatat cccggcaagt ctaagtgctt taagtctttt
      541 agcaagacgc ggtgtgcttt ttaaaggtgc ttcttcctta gctctatttg cggatgtaga
      601 agcagttgct tttgataaaa ctggtacttt aacggaaggg aaaccgagtg ttactgatgt
      661 taatatcccg ccaggagtaa gtgcagaaga agcagaagaa ggaaaagttt tccttgtcag
      721 tatggaaaag aaaagtaatc acccattagc gacagcgatt gttagtcatt ttactcctat
      781 aaaaacagtt gatttagaag ttgaacataa aattggcagt ggattacgcg caatgtatga
      841 cggtaacgag tatcttgtag gtaaaccagc attatttaat ttaccagagc gctgcgctaa
      901 tcgcgctaaa gagttggcag ttcaaggtaa aacagtcgtc tttattgcaa aaaacgggaa
      961 accaatctta cttatagcgc ttttagatat gattaaatgt tgtgcctttg atgggcttga
     1021 atactttaga aaacaagaaa ttaatacagt aatgattact ggagataatg aattaacggc
     1081 aaaagctatt ggtaaagagt taggcattac gcgaatatac gcgaatgtat tacctgaaca
     1141 aaagcttgat attgttaaag aactaaaaaa ggaatacggc ggagtagcga tggtgggaga
     1201 cgggattaat gatgcgcccg cgcttgttga agccacaatc gggattgcaa tgggggatgg
     1261 cactgatgtg gcaattgatg tcgctgatgg ggtgcttgtc aacaatgatt tccaaaagtt
     1321 atcattaatg cacgctattt cacgaaaact taaacgcatt gttattcaaa atattgtgct
     1381 agcgctcagt gtggtgctat tattaattgc gcttaatttc gtggtgacat tgccaatggg
     1441 attaacagta gcgatccatg aaggtagtac tattttagtg atattaaacg gtttacggct
     1501 cttaaatttt aatgaaaaac atgcgattaa aaaaccaaaa agaagcaaat ttaacaaaaa
     1561 aatagcctaa tttttggctt tttacgaagt aattagtaaa aatttacgaa atttagcact
     1621 taaatgttga cagtgctatt ttctttagta taatatattt agcaatcgaa agttgcaagt
     1681 gctaaggagg agttatgaga tacagacgaa gagagttaat cttaaagtat actgttgagt
     1741 tatttattaa gactgcccaa cctgttggtt caaagtcatt aatcgataat tataacttaa
     1801 actatagcag tgcaacggtt cgagcggaaa tgaatgccct tgaaggaatg ggctttcttg
     1861 aaaagccaca cgctagtgct ggccgtgtac caagtaagaa aggctatgaa ttctatgttg
     1921 ctaacttacg tgaagatgaa gtgaatgata acttccgaca tcacattgcg acgcttgtgg
     1981 aagagaagac gaaaagcgtt gaacaagtaa ttaatcaaac atgtgaaatt ctctcacaaa
     2041 tgacaaatct tgtcagtgtc gcacttggtc ccgagaaaaa aggtgaaaca ctacaaagta
     2101 ttcaagttgt cgcgttagat aaaaacatgg ggacggcaat atttgtcact aatcgcggct
     2161 atgtcgaaca taataccttc atcgtcccgc cttcaacaag tattcaagaa attagcaaag
     2221 tcgctaatat cttcaatgaa cgcctagtcg gtacaagaat tagtgaagta tcggcaaaga
     2281 tggaactctt acaaccactc ttaaccgatt acattgttga acaagaagtc ttagcaaatg
     2341 ccttcactga tgcatttata aggtacgccc gtgaccggct tagcttatac ggtaaagaaa
     2401 gcttacttga gcaaccagaa ttcattaatg atagtgaaaa gctccgtaaa ctcatatcaa
     2461 tccttgatga tccagagaaa atacgtgaac ttatcacggg aaacggtgac ttaagtgtga
     2521 atatcggggg caaagatgaa gacttaacct tcataagcgc caatgtccat gtcccaggcg
     2581 accaggtcgg acaaattgtc ctagttggtc caaaacggat ggactacagt aaagtcttat
     2641 gggcgttaga atacgtaact gatgagctcg aaaagtactt cgagatagaa agagagatta
     2701 atgatgagtg atgaaaagaa acatgtggaa aaagatgtag cgccaaaaaa acgccatctc
     2761 gcgaaaatac aaaaactcga agctgaaatt gaaaaactaa aagctgaaag tgctgagtgg
     2821 cataacaaat atatgcgcgt actcgctgat atggataatg cgcgtaaaca aaatgcgaaa
     2881 gatcaacaat attacattaa ataccgtgcc gtaccattta ttgaaaaact cttaccagcg
     2941 cttgatatct tcaatcatgt cattcgcgaa gaacaagaag atccagtgct taataactac
     3001 ttaacgggct tccgcttcgt ctacggacaa ctacttgatg ccttaactag tgaaggccta
     3061 gaggaagtag ttgtgaatgt cggcgataaa ttcgatgaaa actcgatgca cgctgtggaa
     3121 gttaaataca gtgaagaagt tgaaccaaac accgtcc
//
LOCUS       DAOPCD010000110         3081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_25166_length_3081_cov_3.585261, whole genome shotgun sequence.
ACCESSION   DAOPCD010000110 DAOPCD010000000
VERSION     DAOPCD010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25166_length_3081_cov_3.585261"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PLQ55_03360"
     CDS             <1..457
                     /locus_tag="PLQ55_03360"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPK29002.1"
                     /translation="ALTVNNRNRDKESKLLASMAIGAYSKAVKNAHTPADWLSYNEDN
                     VNTYLAHPLDGKPSSKGFYLELLRGTSNLYKKKHYVDVPRDLPIFIIAGAEDPVGANG
                     KGIIKLGKFYAKLGFTNLEVKIYEGMRHEILNETDRRDVYADVLSFLMR"
     gene            475..957
                     /gene="smpB"
                     /locus_tag="PLQ55_03365"
     CDS             475..957
                     /gene="smpB"
                     /locus_tag="PLQ55_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPK29003.1"
                     /translation="MAKQKKTNNVITENRRASHDYFLSDFLEVGIVLKGTEIKSLRHS
                     GASLKDTYIVFRNGEAFIEGLYIAPYAFGNIFNHEPRRTRKLLMHKREIARYSANVQI
                     ASMTCVATKMYFVRGRVKLTIALGKGKKLHDKRDAIREREDKLAMAKALKQERSSRDD
                     "
     gene            950..2170
                     /locus_tag="PLQ55_03370"
     CDS             950..2170
                     /locus_tag="PLQ55_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPK29004.1"
                     /translation="MTKEQIKEYLTNTPTRYDKRAYDSFLKEHGIPKLPPAIHITGTN
                     GKGSTAYYLALSLQNSGYNVGLYTSPSFKNVNELACINGEEISDEYISECIKKYDEDF
                     KKYELSLFEIMTFISFNYFADNNLDYVVIEVGMGGLLDATNVFTPLISIITNIGLDHL
                     DTLGSAIMDVAEHKAGVIKKGVPVLLGDAVRRAEKVVLDTALKLNAPVYRTHNNALVT
                     RHDLERINFTYNGEHYTLYSGALYEVNNAVAALDAIEILKSLGVSIKKSAIKAAFKTK
                     PFEGRFNVIQKEPAIIVDGLHNPHAIRALLSDIKVYGKNLKVVFAAFNDKDYFQEIDL
                     LILAGATITLTTFDHPRAVRDFGSTQLPYIGEHKVAIKEAVKSLKKNDMLLIVGSLYF
                     ARLVIDEFKRGEYA"
     gene            2163..2975
                     /locus_tag="PLQ55_03375"
     CDS             2163..2975
                     /locus_tag="PLQ55_03375"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPK29005.1"
                     /translation="MLKTKRLVTALTLSAFLIAISVVIQRFLVIPFGMPSLYRLSLGN
                     IPIIMASLYLGSVFGAIVGAASDLIGATLFPVGTLIIWPVISSTLYGVVPWLILRLVM
                     YLDRKIKVPLFYVFLAIIFIGLETYIFVKPSIRHPFNSTLDPIMFTTTFRIVFTLVLL
                     LIFSGLIITFNVLVKKYKEGAYEKYTGAPTSLAFTLMLMTFFVDILYSSWWKMFQFKV
                     DFFVSVFFHTLIMFILLPFQVVLLLILSNVYAKSRVAELLALPPKEHIDTDD"
BASE COUNT          975 a          507 c          591 g         1008 t
ORIGIN      
        1 cgctctcacc gttaataatc gtaaccgtga taaagaaagt aagttacttg ctagtatggc
       61 aattggtgct tactcaaaag ctgttaaaaa tgcgcatact cccgctgatt ggcttagtta
      121 taacgaagac aatgtcaata cttatctcgc gcatccgctt gatgggaaac cgtcatcaaa
      181 aggtttttat ttagaattat tacgcggcac gagtaattta tataagaaaa agcattatgt
      241 tgatgtcccg cgtgacttac caatctttat tattgctggt gctgaagacc cagtaggcgc
      301 taacgggaaa ggtattatta aactaggtaa attttatgca aaacttggtt ttactaattt
      361 agaagtgaag atttacgaag gaatgcgcca cgaaatatta aatgaaacag atcggcgtga
      421 tgtttacgct gatgtcttaa gtttcttaat gagataaaag aaagatacac tatagtggcg
      481 aagcaaaaaa agacgaataa tgttataaca gaaaaccgcc gtgcaagtca cgattatttt
      541 cttagtgact ttttagaggt gggaattgtt ttaaaaggca cagaaattaa gtcgcttcgc
      601 cacagtggtg cctcgcttaa agatacttat attgttttcc gtaacggaga agctttcatt
      661 gaaggtctat atattgctcc atatgctttt ggtaatattt ttaatcacga accacgcagg
      721 acacgtaaac ttttaatgca taaacgcgaa atagcacgct atagcgcaaa tgtgcaaata
      781 gcgtcaatga catgcgtcgc cacaaaaatg tattttgttc gtggtcgtgt caagcttaca
      841 attgcgcttg gtaaaggcaa aaaacttcat gacaagcgtg atgcaattag agagcgcgaa
      901 gataaattag cgatggcaaa ggctttaaag caagaaagaa gttcgcgtga tgactaaaga
      961 gcaaattaaa gagtatttaa ctaatacccc gactcgttac gacaaaagag cgtatgactc
     1021 atttttaaag gaacacggaa tcccaaaatt accgcctgca attcatataa cagggactaa
     1081 cgggaaggga agtactgcct attaccttgc cttaagtttg caaaatagtg ggtataatgt
     1141 cggcttatat acatccccat cttttaaaaa tgttaacgaa ttagcgtgca ttaatggtga
     1201 agaaattagt gatgaatata ttagtgaatg tattaaaaag tatgacgaag actttaaaaa
     1261 atatgaatta tcactttttg aaataatgac atttattagc tttaattatt ttgcggataa
     1321 taatcttgat tatgtcgtta ttgaagtggg aatgggcggt ttacttgacg ctacgaatgt
     1381 ttttaccccg cttataagca tcattactaa tattggactt gaccatcttg atacacttgg
     1441 atcagcaatt atggatgttg ccgaacataa ggcgggcgtt ataaaaaaag gtgtgccggt
     1501 cttacttggt gatgctgtta gacgcgctga aaaggttgtc cttgacactg ctttaaaact
     1561 taacgcgcca gtgtatcgca cgcataataa tgcacttgtt acaagacatg atttagagcg
     1621 gattaatttt acatacaatg gtgaacatta cacgctttat agcggagcac tttatgaagt
     1681 aaataatgca gtagcggcac ttgacgccat tgagatatta aaaagtttag gtgttagtat
     1741 taaaaaaagt gcgattaaag ctgcatttaa aactaaaccg tttgaaggtc gttttaatgt
     1801 tattcaaaaa gaaccagcaa ttattgttga tggtttacat aacccacatg caattcgtgc
     1861 tttattaagt gatattaaag tatatggtaa aaatctcaaa gtagtgtttg ctgcttttaa
     1921 tgataaagat tattttcaag aaattgattt attaatttta gcaggggcca caattacttt
     1981 aacaactttt gatcatccgc gggctgtcag agattttgga tcaacacaac ttccgtatat
     2041 tggtgagcac aaagtggcaa ttaaagaagc agtgaaatca ttaaagaaaa atgacatgct
     2101 tttaattgtt ggttcgcttt attttgcgcg ccttgttatt gatgaattta aaagaggtga
     2161 atatgcttaa aacgaaacga ctcgtgacag cattaacact aagtgctttt ttaattgcta
     2221 tcagtgtcgt tattcaacgc tttttagtaa ttccttttgg aatgccttca ctttaccgct
     2281 tatcacttgg taatattccg attattatgg cttcacttta tcttggctcc gtatttggtg
     2341 cgattgtggg cgcggcaagt gatttaattg gggcgacact tttcccggtc ggaaccctta
     2401 ttatttggcc agtgattagt tcaacacttt atggtgttgt gccgtggctt attttacgcc
     2461 tagtgatgta tttagataga aaaatcaaag taccgctttt ctatgtattt ttagctatta
     2521 ttttcattgg cttagaaaca tatatctttg ttaagccttc aattagacac ccgtttaata
     2581 gcacgcttga cccgattatg tttacgacaa cttttagaat tgtttttacg cttgttttac
     2641 tccttatctt tagcggactc attattactt ttaatgtgct tgttaaaaaa tataaagaag
     2701 gtgcttatga aaaatatact ggtgcaccaa cttcactagc gtttacactg atgctgatga
     2761 ctttctttgt cgatattctt tattcgtcat ggtggaagat gttccaattt aaagttgatt
     2821 ttttcgtcag tgtctttttc cacactttaa tcatgtttat tttattacca ttccaagttg
     2881 tgcttttatt aattttaagt aatgtatacg ccaaaagcag agtagcagaa cttttagcgc
     2941 tacccccaaa agaacatata gatacagacg attaagcctt tttataatgt tttaaacctt
     3001 aagttaggcg tgaattaacg ccttttttaa aaactactaa aaaactccta aacgcattag
     3061 acacaatatg tcaaaattaa t
//
LOCUS       DAOPCD010000111         3054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_25447_length_3054_cov_3.299433, whole genome shotgun sequence.
ACCESSION   DAOPCD010000111 DAOPCD010000000
VERSION     DAOPCD010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25447_length_3054_cov_3.299433"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLQ55_03380"
     CDS             <1..577
                     /locus_tag="PLQ55_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29006.1"
                     /translation="SRDFLLNYPVDDYDFATDATPAEMHEFLPDANYRFEKFGTVSLK
                     YEDEHVEITTLREEGEYIDSRHPAYIKFVTDPAKDYIRRDFTINALYIDENYRVLDFC
                     DGLKDLDTNTLRVIGNPYTRFAEDPLRIIRALRFVAKFKLNIDPLTENAIRDLAHLVK
                     KLNPEKVKAEIRKVGEQEQNIFKKLMNMYKL"
     gene            632..2419
                     /gene="glmS"
                     /locus_tag="PLQ55_03385"
     CDS             632..2419
                     /gene="glmS"
                     /locus_tag="PLQ55_03385"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPK29007.1"
                     /translation="MNGFIGRVGNSCVKEKLLSALNLLQFKDDDSTGVLFCNKEGQHL
                     YRVVGKVDELVTLIPEEVDGYMGIAHTRWSTYMHQVLENVYPLSSENGYVTLVVNGLI
                     DNVQMIKRRLVKRGYSFRSSSAVEVVANLIEDGAVENEDPLRTLHRAMQQLEGDYALL
                     AVFNGNCKKIFFAKNGVPLIIGKNHDGMVICTDYTPIADQADEYYYPNDGELGYLTSD
                     NMVLYDGKLKQRKPSFTEVSVSEKTLELGDYPHYMLKEIEEAPGVIRRLINHYFDGTK
                     YRFDDRLIKRIIDAGTIVFIAAGTSYNAALVGQRYLRNFNKKVDVFIASEWIFYPYQS
                     GEDTLYVLISQSGETADILKCLRTIRAYGADVLAITNVEVSTLYQSADYRLLLHAGTE
                     VSVASTKAYIAQITLLSLLYARLVNKITTIAALERVIEALYNIISRRDTIKAIADQIY
                     KSGDVFFLGRGFDYDVAVEAQLKLKETSYIHAEAYPGGEMLHGPIALIEENTPVIFFI
                     SDSLTVAATREVIREMRDRKANVIVCSLAPFNHDGDSFIISNPIKGYHSPITFAVFAQ
                     YLAYYCAVNLGRDVDKPRNLTKAVKDRSV"
     gene            2412..>3054
                     /locus_tag="PLQ55_03390"
     CDS             2412..>3054
                     /locus_tag="PLQ55_03390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HPK29008.1"
                     /translation="MYNKALDFRFMLFEYYKELRINETELVIILFIEHLQQKETEFIT
                     PDLINLQSNIDIKVIDEAMVRLVSKGLIEFTTRNGKMVTSLNPLRNKLQELFVLDYEK
                     NKENSTVEFSSQVEEVYAFIQNAFGRSLSAVELQTINDWFNYGYNITMIKEALDQTMK
                     RKRYNIRAIDKALLKLSTAVDYALEGKSAQSDSYRKNIKDTLEEVNKKAQDDEN"
BASE COUNT         1003 a          488 c          606 g          957 t
ORIGIN      
        1 aagccgtgat tttcttttaa actacccggt cgacgactat gattttgcga cagacgctac
       61 accagcggaa atgcatgaat ttttgcctga cgcaaattat cggtttgaaa agtttgggac
      121 agtaagttta aagtatgaag atgaacatgt ggaaattaca actttgcgcg aagaaggtga
      181 atatattgat agtcgccacc ccgcttatat aaagtttgta acagaccccg ctaaagatta
      241 tattagacgt gattttacga ttaacgcact ttatattgat gaaaattatc gagtgcttga
      301 tttttgtgat ggcttaaaag atttagatac aaatacttta cgcgttattg gcaatcctta
      361 tacccgtttt gcagaagatc cgctgcggat tattcgggca ttgcgatttg ttgctaagtt
      421 taaattaaat attgacccct taactgagaa cgcgattcgt gatttagcgc atcttgttaa
      481 aaaactaaat ccagaaaaag ttaaagctga aatacgaaaa gtgggagaac aagagcaaaa
      541 tatctttaaa aaattaatga atatgtacaa attataagat ttacattgcg taataatgta
      601 aactattaat accaaaagtg agggaatctt tatgaatgga tttataggaa gagttggtaa
      661 ttcgtgtgta aaagagaaac tgttaagtgc gttaaactta cttcagttca aagatgatga
      721 ctccacagga gttctttttt gcaataaaga aggccaacat ttatatcgtg tagtaggaaa
      781 agtagatgaa ttagtcacac ttattccgga agaagttgat ggttatatgg ggattgctca
      841 cactagatgg tcaacataca tgcatcaagt tcttgaaaat gtttatccgc tctctagtga
      901 aaatggttat gtcacccttg ttgttaatgg gcttattgat aatgttcaaa tgattaagcg
      961 tcgacttgta aaacgcggat atagttttag atctagttcc gctgttgaag ttgtcgctaa
     1021 tttaattgaa gacggcgctg ttgaaaacga agacccacta cggacactcc atcgcgctat
     1081 gcaacaacta gaaggtgatt atgccttgct tgctgtcttt aacggtaatt gtaagaaaat
     1141 attttttgct aaaaatggtg ttccgctcat tattggaaag aatcatgacg ggatggtcat
     1201 ttgtacagat tacaccccaa ttgccgatca agccgatgaa tattattatc caaatgacgg
     1261 agagttaggc tatttaacaa gtgataatat ggttttatat gacggcaaac ttaaacaaag
     1321 aaaaccgtct tttaccgaag tgtcagttag tgaaaaaaca cttgagttag gtgattatcc
     1381 acactatatg cttaaagaaa ttgaagaagc tcctggtgtt attcgtcgcc ttatcaacca
     1441 ttactttgat ggaacgaaat atcggtttga tgaccggctt attaaacgaa ttattgatgc
     1501 gggaacgatt gtctttattg cggctggtac tagttacaat gccgctttag tgggacaacg
     1561 ttatctacga aactttaata agaaagttga cgttttcatc gctagtgaat ggatttttta
     1621 tccgtatcaa tcaggtgaag acacattata tgttttaatt tcacaatcag gtgaaacagc
     1681 ggatattctt aaatgtttac gcacaattag agcatatggc gctgatgttt tagcaattac
     1741 taatgttgaa gtttctacac tttatcaatc agcggattat cgcttattac tacacgctgg
     1801 tacagaagtt agtgttgcta gtacaaaggc ttatatcgcg caaattacac ttctttcttt
     1861 gctttacgcg cgcttagtaa ataaaattac gacaattgca gcattagaaa gagttattga
     1921 agcgctctat aacattatct cgcgtcgtga tacgattaag gcaattgccg atcaaattta
     1981 taagtcaggc gacgtctttt tcttagggcg cgggtttgac tatgatgttg ctgtagaagc
     2041 acaacttaaa ttaaaagaaa ctagctatat tcatgctgaa gcttatccgg gcggagaaat
     2101 gctgcatggc ccaattgcgc ttattgaaga aaatacacca gttatcttct ttattagtga
     2161 tagtttaact gtagcagcaa cgcgggaagt tattagagaa atgcgtgatc gtaaagctaa
     2221 tgtaattgta tgctcacttg cgccatttaa tcatgatggt gatagcttta ttatttcaaa
     2281 cccaattaag ggttatcata gtccaatcac ctttgcagta ttcgcgcagt atcttgctta
     2341 ttattgtgca gttaacttag gacgtgatgt tgataagcca cgtaatttaa caaaggcggt
     2401 taaagatcgt agtgtataat aaggcgctag attttcgctt tatgcttttt gaatattaca
     2461 aagagctccg tatcaatgaa acagaacttg taatcattct ttttattgag cacttacaac
     2521 aaaaagaaac agaatttatt actcctgatt taattaatct tcaatcaaat attgatatca
     2581 aagtgattga cgaagcaatg gttcgtcttg ttagtaaagg tttaattgaa tttacgacta
     2641 gaaacgggaa aatggttact tcacttaatc cgttacgaaa taaattacaa gaacttttcg
     2701 tgttagatta tgaaaagaat aaagaaaatt cgacagtaga atttagttca caagttgaag
     2761 aagtttatgc ctttattcaa aatgcttttg gccgttcact ttcagcagta gaactgcaaa
     2821 cgattaatga ttggtttaat tatggttata acatcacaat gattaaagaa gcacttgacc
     2881 aaacaatgaa acgtaaacgc tataacattc gcgctattga taaggcgcta cttaaattat
     2941 cgactgctgt ggattatgcg cttgaaggta aaagtgccca aagtgattca tatcgaaaaa
     3001 acatcaagga cacacttgaa gaagtgaata aaaaagccca agatgacgag aacg
//
LOCUS       DAOPCD010000112         3007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_25933_length_3007_cov_3.246612, whole genome shotgun sequence.
ACCESSION   DAOPCD010000112 DAOPCD010000000
VERSION     DAOPCD010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25933_length_3007_cov_3.246612"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..506
                     /gene="rlmH"
                     /locus_tag="PLQ55_03395"
     CDS             30..506
                     /gene="rlmH"
                     /locus_tag="PLQ55_03395"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPK29009.1"
                     /translation="MKIDIIVVGKTKGALLITSDEYTKMISKYADISIIEIKEERLPL
                     RASEAQIENALNREAETILKRIGERDFVYALSPEGIEFDSIMFSEQLRNAFDIGQSKV
                     MFVIGSSHGLGNEVYKRANLKLSFSKMTFPHQLFRVMLLEQIFRAFKIINNETYHK"
     gene            521..898
                     /locus_tag="PLQ55_03400"
     CDS             521..898
                     /locus_tag="PLQ55_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29010.1"
                     /translation="MANNTYNYDNIVPEWNYSEFKHLKRVSNPRTAFARGYLFEEGEF
                     YIEPWFYTQLTRILERFRNEHDEIMDVFFNIARKEKYVLFTRDINEPIFDDENYLLVE
                     IEDIIEGAKLIIDDNSRGSDYGD"
     gene            888..1748
                     /locus_tag="PLQ55_03405"
     CDS             888..1748
                     /locus_tag="PLQ55_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HPK29011.1"
                     /translation="MAIKGIFVTNISHNSPKFRIQVDEFLNKAKLIPNLELIEVKNQH
                     VLSYLNNNTPDFCLFWDKDIYLAKRIENSGIKVFNNPETIYLCDDKALTAAELAKARV
                     AQPRFFVFPLHYWGNIIEYYEEYKEELLALSFPLVIKERFGSFGDQVYLAHSEEELKK
                     IIKEHGTKGLLAQEFVSGEKGTDYRVNVVGDKVIFTVKRVNDKDFRSNINQGGTASLV
                     KATSEIEDLAIQATKALKGHFAGVDIMLDAKGKPVVIEVNSNMRTVAVNKVSDIDLTL
                     AILSYIVSNI"
     gene            complement(1813..>3007)
                     /locus_tag="PLQ55_03410"
     CDS             complement(1813..>3007)
                     /locus_tag="PLQ55_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29012.1"
                     /translation="YIHMSRKFKQKPKKVKAEKVKREPDMRKRAYLAMLFNNRAAFDG
                     GRREPWWVAVLFFIASIVIALVPAMVQVGKTKGSDIFKGPLYHTDVAFTKFVETLEEK
                     DADLTVVSENDENIFKASPEFVNLVANKAFTLTDGATNEVVPYYSFAQKRIVYTRDEN
                     NAVVTNEVDFEYLRVYYTGDIQSSFLLEGKVYSGDAFLALKLLSLKEEDAVGNVTSHL
                     IIGRKALYTRLYNPTAINKPGNPALVFEGRTNSLPVGMNIRDFGKVSKDGVPLAKTDI
                     DYTDKVMENFGHMQDLGYKEVKVRTFWFQTGIYAAIFSIIGLVMGLIIFISTRGKMNP
                     NRYLKFGESLKIGAWLLPAPALITLVLGFILPAQYFQMIFIMTLGMRSVWLTMRTLNP
                     NMPQQ"
BASE COUNT         1042 a          486 c          538 g          941 t
ORIGIN      
        1 acaacatgtg ccaattatgg gagatttaaa tgaaaattga tattattgtt gtcgggaaaa
       61 caaaaggtgc acttctaatt actagcgacg aatacactaa aatgattagt aaatatgctg
      121 atatttcaat tattgaaatt aaagaagaac gacttccgct tcgcgcaagt gaagcacaga
      181 ttgaaaatgc attaaataga gaagccgaaa ccattttaaa aagaattggt gaacgcgact
      241 ttgtttatgc cttgtcacct gaaggaatag agtttgattc aattatgttc agcgaacaat
      301 tacgtaacgc ctttgatatt ggtcaaagta aagtaatgtt cgttatcggt agttcccatg
      361 gtttaggaaa tgaagtatat aagcgtgcga acttaaaatt aagttttagc aaaatgacgt
      421 ttccccatca actttttaga gtaatgctcc ttgaacaaat atttagagca ttcaaaatta
      481 taaataacga aacatatcat aaataaggag aaatattaat atggcaaata atacttacaa
      541 ttatgacaat attgttccag agtggaacta tagcgaattt aaacatttaa agcgtgttag
      601 taacccacgg accgcttttg cgagaggata cttatttgaa gagggtgaat tttatattga
      661 accatggttt tatacacaat taacgagaat cttagaacga tttagaaatg aacatgatga
      721 aataatggat gtatttttta atattgctcg taaagaaaaa tatgtattat ttactcgtga
      781 tattaatgaa ccaattttcg atgacgaaaa ctatcttttg gtggaaattg aagatataat
      841 tgaaggtgca aaattaatta ttgatgataa ttcacgcggg agcgattatg gcgattaagg
      901 gcatttttgt cacgaatatc agtcataata gcccaaaatt tcgcattcaa gttgatgaat
      961 ttttaaacaa agctaaactt ataccaaatc ttgaattaat tgaagttaaa aatcaacatg
     1021 tcttaagtta tttaaataat aatacacctg acttttgtct tttttgggac aaagatatat
     1081 atctagcaaa acgtattgaa aatagcggaa ttaaagtttt taataatcct gaaacaattt
     1141 atctctgtga cgataaagca ttaaccgctg cggagcttgc taaagcgcgt gtagcgcaac
     1201 cacgcttctt tgtatttccg cttcattact ggggcaatat tattgaatat tatgaagagt
     1261 ataaagaaga gttacttgca ttatcttttc cccttgtcat taaagaacgc tttggttcat
     1321 ttggtgatca agtttattta gcgcatagtg aagaagagtt aaaaaagatt ataaaagagc
     1381 acggcacaaa aggacttttg gctcaagaat ttgtcagcgg cgaaaaaggg acagattacc
     1441 gtgttaatgt tgtgggagat aaggtaatat ttactgttaa aagagtcaat gacaaagatt
     1501 ttagaagtaa tattaatcaa ggcggcactg cttcattagt aaaagccact agcgaaatag
     1561 aagatttagc gattcaggca acaaaagcat taaaaggtca tttcgcgggt gttgatatta
     1621 tgcttgacgc taaaggaaaa ccagtagtga tcgaagtaaa ttcaaatatg cgcacggttg
     1681 cagttaataa agtaagtgat attgatttaa cactagcgat attaagttat attgttagta
     1741 atatttaatg tacataaaag ttaaaaaaat caccttgttt ttagcaaggt gaatttttat
     1801 taattcttaa tgttattgtt gcggcatatt tggatttaat gtccgcattg ttaaccatac
     1861 actgcgcata ccaagcgtca taatgaaaat catttggaaa tattgcgctg gtaaaataaa
     1921 gccaagcacg agtgtgatta aagctggagc aggaagtaac cacgcaccaa ttttaagtga
     1981 ttcaccaaac ttaagatagc gatttggatt catttttccg cgtgtagaaa taaagataat
     2041 taaacccata acaagaccaa taatagagaa gatagcagcg taaattccag tttgaaacca
     2101 gaaggtccgt actttaactt ctttataacc taagtcttgc atatgaccaa agttttccat
     2161 aactttatca gtgtaatcaa tatcggtttt ggcaagtggg acaccatctt tactcacttt
     2221 accaaaatca cgaatattca taccaactgg taatgaattt gtccgtcctt caaacactaa
     2281 agcagggtta ccaggtttat taatggccgt tggattataa agtcttgtgt ataatgcctt
     2341 ccgcccaata attaaatgtg aagtaacatt accgacagca tcttcttcct ttaatgataa
     2401 aagtttaagc gctaaaaaag catctccact ataaactttt ccttcaagta aaaacgaact
     2461 ttgaatatca ccagtgtaat aaacgcgtaa gtattcaaaa tcaacttcat tagtaacgac
     2521 agcattattt tcgtcacgcg tatacactat acgcttttgc gcgaaggagt aataaggaac
     2581 aacttcattc gtggcgccat ctgtcaaagt aaaggctttg ttcgctacta ggttaacaaa
     2641 ttcaggacta gccttaaaga tattctcatc attttcactt acaactgtaa gatcagcgtc
     2701 tttttcttca agtgtttcta caaacttcgt aaacgctaca tcagtgtgat ataaaggccc
     2761 tttaaagatg tcgcttccct tagtcttacc aacttgtacc attgctggca ctaaagcaat
     2821 aacgattgaa gcgataaaaa agagcactgc gacccaccac ggttcgcgtc ttccgccgtc
     2881 aaatgcagcg cgattattaa aaagcatcgc taagtaagcg cgtttacgca tatcaggttc
     2941 gcgttttact ttttctgctt ttactttttt aggtttttgt ttaaattttc tactcatatg
     3001 tatatac
//
LOCUS       DAOPCD010000113         2977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_26248_length_2977_cov_2.811088, whole genome shotgun sequence.
ACCESSION   DAOPCD010000113 DAOPCD010000000
VERSION     DAOPCD010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26248_length_2977_cov_2.811088"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="PLQ55_03415"
     CDS             complement(<1..1156)
                     /locus_tag="PLQ55_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HPK29013.1"
                     /translation="MANKNTAKRYELPLIITRNQVYFPFNQSDDLDAGRPATVEAIKY
                     ANNSDDKLLIVATQKVFTVNNPSMNDIYEVGVLARLTKVRERPHHITVQLEPLARVFI
                     NELDESGPFIIADATVTFHEPLDPDANPERLKNALFDFFNDKLELTRRLHIDLDALKA
                     ADMNLEELIYFLANKTLNSTEQRMAVLAENSLERRIDILLKFLEDDVANDAVGAITQI
                     VRNRPQSGEGLDEVERDGLPLEDDEDEDEDEEFDTGEEILAQLKKRYFPKNFVTRIKK
                     EAKKMGRVEPSERSRIIEYLDWLMKLPYDQETIDNLDLENVRKVLDKDHFGLEEPKKR
                     IVEFMAVKRLAKENRATVLCFYGPPGTGKTSLATSIATAMGRKLVKASLGG"
     gene            complement(1200..2474)
                     /gene="tig"
                     /locus_tag="PLQ55_03420"
     CDS             complement(1200..2474)
                     /gene="tig"
                     /locus_tag="PLQ55_03420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPK29014.1"
                     /translation="MKLKVTKLETSKYELEIEVSEAKWSAAREKAFKKLEKDVTVQGF
                     RKGKAPEHLVRAKIDPVKELDEAVHSLLDEAFQFAIKESGHYPVIQPTYDVVKVSDTE
                     LVLKFIIVAAPVVTLGQYEGLKIGRGEANVEDSAVEVRLNSILEQNAELILKEDAAAL
                     GDTVVLDFEGFVDGEAFEGGKATNHELELGSGQFIPGFEDALVGVKAGESRDVNVTFP
                     ENYHEQLAGKKAVFKTTVHEVKEKRLPALTDDFAASLNMPNVTDVASLRVHVLFELTK
                     QAEAAERNEYVEKILAKLRETSTFELANDIIDREVEHMDERLKNQVTQQGMEWKQYLE
                     LTKQTEEEIKERFVSEATVNLQNFLVLNEIAKAKEIEVTDAEVDFEIAMMAQQYNMEE
                     KRIRELLGDNINNLRNDVRQKKIINFLLENNE"
     gene            complement(2618..>2977)
                     /locus_tag="PLQ55_03425"
     CDS             complement(2618..>2977)
                     /locus_tag="PLQ55_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29015.1"
                     /translation="TVQVNKFGKLIKVNPATAPDPFKTKGGAKLIKKMQEVAALDDYN
                     QFSEVADSLITGHAMYIYPLTYEVKDVDGLGDAYLYYIYRALGRVNNVNEYIEEHGLN
                     AETLFAIFTKYHFTYFD"
BASE COUNT          830 a          604 c          508 g         1035 t
ORIGIN      
        1 ccccacctaa actagctttg acaagtttac ggcccatcgc ggttgcaata cttgtcgcta
       61 gactagtttt accagtgcca ggtggtccat aaaaacataa aaccgttgct ctattttctt
      121 tagcaaggcg ttttacggcc ataaattcga caatgcgttt ttttggttct tctaaaccaa
      181 agtgatcttt atctaagacc ttacggacat tttcaaggtc aagattatca attgtttctt
      241 ggtcataagg taacttcatt aaccaatcta aatattcaat tatccgtgag cgttcacttg
      301 gttcaacgcg gcccatcttt ttcgcttctt ttttaatgcg cgttacgaag tttttgggga
      361 agtagcgctt tttaagttgc gctagaattt cttcaccagt atcaaattct tcgtcttcgt
      421 cttcatcttc atcgtcttct agcggtaagc cgtcgcgttc aacttcgtca agtccttcac
      481 cgctttgtgg gcggttacgc acaatttgtg taatcgctcc aacagcatca ttagcgacat
      541 cgtcttcaag aaacttgagt aaaatatcaa tgcgtcgctc tagactattt tcggcaagga
      601 cagccattct ttgttcggtg ctattgagcg ttttattcgc taagaagtaa ataagttctt
      661 ctaaattcat atcggcagcc tttaaggcat ctaaatcgat atgtaaccgt cttgttaact
      721 ctaatttgtc attaaagaaa tcaaataagg cattttttaa gcgttcagga ttagcgtcag
      781 ggtcaagtgg ctcatgaaaa gtaacagtag cgtcagcaat aataaaggga ccactttcat
      841 caagttcatt aatgaaaaca cgggcaagtg gttcaagttg tacggtaatg tgatgcggtc
      901 tttcacgcac ttttgttagt cgtgcgagta cgccgacttc ataaatatca ttcatcgatg
      961 ggttattaac cgtaaaaacc ttttgcgttg ctacaattag taacttatca tcactattat
     1021 tagcgtattt aatcgcttca acagtcgccg gtcgtcccgc atctaagtca tcactttgat
     1081 taaacggaaa atacacttga ttgcgtgtaa taataagcgg taactcatag cgttttgctg
     1141 tatttttgtt agccatgtaa cctctccttt tctttatgta aaacttaact aattaataat
     1201 tattcattat tttctaataa gaaattaata attttctttt ggcggacatc attacgtaaa
     1261 ttgttgatgt tatcgccaag taattcacgg atgcgttttt cttccatgtt atattgttgc
     1321 gccatcatcg caatttcaaa atcgacttcc gcatcagtaa cttcaatttc tttagctttg
     1381 gcaatttcat taagcactaa gaagttttgt aagttaacgg ttgcttcact cacaaagcgt
     1441 tctttaattt cttcttcagt ttgttttgtt aattctaaat attgtttcca ttccattcct
     1501 tgttgcgtga cttgattttt aagtctttca tccatatgtt cgacttcacg gtcaataatg
     1561 tcattcgcaa gttcaaaggt tgatgtttcg cgtaatttcg ctaagatttt ttcaacatat
     1621 tcattgcgtt cagcggcttc ggcttgttta gtaagttcaa ataagacatg gacgcgtaaa
     1681 ctagcaacat ccgtaacatt tggcatatta agtgaggctg caaagtcatc agttaacgct
     1741 ggtaaacgtt tttctttaac ttcatggact gttgttttaa agacagcttt cttaccagcg
     1801 agttgttcat gataattttc ggggaaagta acgttaacat cacggctttc acctgctttt
     1861 acaccgacaa gtgcatcttc aaaacctggg ataaattgac cactgccaag ttctaattcg
     1921 tgattagtgg ctttaccacc ttcaaaagct tcgccatcga caaacccttc aaagtcaagc
     1981 acaacagtgt cacctaaagc agcagcgtct tcttttaaga taagttcggc attttgctct
     2041 aagattgagt taaggcgtac ttcaacggca ctgtcttcaa cattagcctc accacgaccg
     2101 attttaagcc cttcatattg gccaagtgta acaactggtg cagcaacaat aataaattta
     2161 aggacaagtt cagtatcact tactttaacg acgtcataag taggttgaat tactggataa
     2221 tgtccgcttt ctttaatcgc aaattggaaa gcttcatcga gtaaactgtg cactgcttca
     2281 tctaactctt taacgggatc aattttagcg cgcactaagt gttctggtgc tttaccttta
     2341 cggaagcctt ggacagtgac gtctttttct aatttcttaa aggctttctc acgtgccgca
     2401 ctccatttag cttcactaac ttcgatttct aattcgtatt tacttgtttc aagtttagtt
     2461 acttttaatt tcatatataa aaattcctcc atttataacc ttagtaagta tactaaaata
     2521 tcgctcattt tagtaataaa gtggcttatt tatgtaatat ttccgtgatt aaggacaatt
     2581 taatcatttt atttcgttaa atcataaaaa gacatgatta atcaaagtag gtaaagtgat
     2641 actttgtaaa tatcgcaaat agtgtttcag cgtttagacc gtgttcttca atatattcat
     2701 taacattatt aacacgccct aacgcacgat aaatgtaata aaggtaggcg tcccctagtc
     2761 cgtcaacatc ttttacttcg taagtaagcg ggtaaatata catcgcatga cctgtaatta
     2821 aagaatcagc gacttcacta aattgattat agtcatcaag cgccgctact tcttgcattt
     2881 ttttaattaa tttagccccg cctttcgttt taaaaggatc aggcgctgtt gcggggttta
     2941 ctttaattaa tttcccaaac ttattcactt gcacagt
//
LOCUS       DAOPCD010000114         2904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_27069_length_2904_cov_2.784135, whole genome shotgun sequence.
ACCESSION   DAOPCD010000114 DAOPCD010000000
VERSION     DAOPCD010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27069_length_2904_cov_2.784135"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLQ55_03430"
     CDS             <1..259
                     /locus_tag="PLQ55_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29016.1"
                     /translation="LTTKLPNILRVFDMGDALAFKEEEHYYIIIKRRSHLPIYNWRYF
                     ELVIRSVCEIKNMTLISALEIEDNPFKAYFIEKGTLIKRAI"
     gene            260..586
                     /locus_tag="PLQ55_03435"
     CDS             260..586
                     /locus_tag="PLQ55_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29017.1"
                     /translation="MNQSEIMKNLKQEIKSSYEGFLKLGIHDEQAFHEHKEAIAFSVH
                     TVKAIAKILEPIPNEILYFKGGIGDTLAAVKEYAKNLVSKFGTLNTYKLYDFMSIDLP
                     TLRDTL"
     gene            complement(664..1023)
                     /gene="rplT"
                     /locus_tag="PLQ55_03440"
     CDS             complement(664..1023)
                     /gene="rplT"
                     /locus_tag="PLQ55_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPK29018.1"
                     /translation="MARVKSSPVTRARRKKVLKQAKGYFGAKSLLFRTAKEQVMHSLR
                     YAYIDRRRIKRDYRKLWIKRINAGCRMCEISYSQFMHGLKLANVDINRKMLSELAIND
                     FETFKGLTETAKKALAK"
     gene            complement(1036..1230)
                     /gene="rpmI"
                     /locus_tag="PLQ55_03445"
     CDS             complement(1036..1230)
                     /gene="rpmI"
                     /locus_tag="PLQ55_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPK29019.1"
                     /translation="MPKMKSKSALTKRIKVTKSGKLMRKAAYTSHLAPRKTTKQKRQL
                     RKARLVHKSDAKRIKYLIQN"
     gene            complement(1242..1793)
                     /gene="infC"
                     /locus_tag="PLQ55_03450"
     CDS             complement(1242..1793)
                     /gene="infC"
                     /locus_tag="PLQ55_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPK29020.1"
                     /translation="MLIYNQNRERPERNTDLVNENIRFPEVLVIGPNGEQLGVMKTRA
                     AIDLAASEYELDLYCVNPNGKPPVCKMLNYSKHRFEQQKRAKEQKRKQKTVELKEIRM
                     SPVIDVHDQETKGRMARRFLEDGNKVKVTIRYRGRQMAHIDVGEKILESFVASLEDYA
                     IVERKPLMEGRNLSVILASKIKK"
     misc_feature    complement(1802..1914)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(1966..2577)
                     /locus_tag="PLQ55_03455"
     CDS             complement(1966..2577)
                     /locus_tag="PLQ55_03455"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPK29021.1"
                     /translation="MAFTLKPLPYAYDALEPYIDAKTMEVHHSKHHATYVANLNAALA
                     KDPSFNDWSLEKILKNLDKLPESIRTAVRNNGGGVYNHDLFWTLLKVNNGKGPEGKLK
                     DALVATFGTFEAFKDAFATAAKSRFGSGWAWLVVTSDKKLKVVSTPNQDIPFDEGTPI
                     LTLDVWEHAYYLKYQNRRPEYVEAFFNVINWDEVARLYENALK"
     gene            complement(2633..>2904)
                     /locus_tag="PLQ55_03460"
     CDS             complement(2633..>2904)
                     /locus_tag="PLQ55_03460"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPK29022.1"
                     /translation="NDAHLKHAKSVVRKLEKQGLRVELDDSNEKLGYRLRNSQINKIP
                     YTLVIGDKERNDNLVTYRKFGDKAQITVPYREFITLIKREIRDKA"
BASE COUNT          855 a          520 c          521 g         1008 t
ORIGIN      
        1 attaactact aaattaccaa atatcttaag agtgttcgat atgggtgacg ctttagcctt
       61 taaagaagaa gagcattatt acatcatcat taaacgcaga tctcacctcc caatttataa
      121 ttggcgatat ttcgaactcg tgattagaag tgtttgcgaa ataaagaata tgacgctaat
      181 ttctgctctt gaaattgaag ataatccgtt taaagcttac tttatagaaa aaggaacact
      241 aattaagagg gccatttaaa tgaatcaaag tgaaataatg aaaaacttga aacaagaaat
      301 aaaaagtagt tatgaaggct ttttaaaact aggtatccat gatgaacaag cttttcatga
      361 acataaagaa gcaatagcct ttagtgtgca tacagttaaa gcgattgcta agatattaga
      421 acccatacct aatgaaattt tatactttaa agggggaata ggcgatactc ttgccgctgt
      481 taaagaatat gctaaaaatc tagttagtaa atttggcaca cttaatacat ataagttata
      541 tgactttatg agtatagatt tacctactct tcgtgacact ctataattat tgaaaagtga
      601 aacattaaaa aagggagtaa acataaatct actcccattt ttattaagaa agaaatattt
      661 atattatttt gcgagtgctt ttttagctgt ttcagttaaa cctttgaatg tttcaaagtc
      721 gttaattgct aattcactta acattttacg attgatatca acatttgcaa gttttaaacc
      781 gtgcataaat tgactataac tgatttcaca catccgacag ccggcgttaa tgcgtttaat
      841 ccataattta cgataatcac gtttaatccg acgacgatcg atataagcat aacgtaagga
      901 gtgcataact tgctctttag cagttctaaa gagtaagctt ttggcgccaa agtaaccttt
      961 agcttgtttt aaaacttttt ttctacgggc acgtgtgacc ggtgaacttt ttactcttgc
     1021 catgattcct tcctcctaat tctgaattaa atacttaata cgtttagcat cactcttatg
     1081 cactaaacgc gcttttcgta attgtctttt ttgttttgtt gttttgcggg gggctaaatg
     1141 cgatgtatat gcagctttac gcattaattt gcctgattta gtcactttaa tccgtttggt
     1201 taaagcactt ttacttttca tttttggcat gaaacttcct cctatttctt aattttactc
     1261 gctaagatga cgcttaagtt acgaccttcc attaacggtt ttcgttcgac aatagcataa
     1321 tcctctagtg atgcaacaaa gctttctaat attttttcac caacatcaat gtgggccatt
     1381 tggcgtccac gataacggat tgtaactttt actttattac catcttctaa gaaacgacga
     1441 gccatccgcc cttttgtttc ttgatcgtgt acatcaatta cgggtgacat acgaatttct
     1501 ttaagttcga cagtcttttg tttccgtttt tgttctttag cgcgcttttg ttgttcaaag
     1561 cggtgtttag aataattaag cattttgcaa actggtggtt tgccgttcgg gttcacacag
     1621 tagagatcta gttcgtattc agatgccgct aggtcaatag cagcgcgtgt tttcatgacg
     1681 cctagttgtt cgccgttagg accaatgact aagacttcag gaaaccgaat gttttcatta
     1741 actaaatcgg tattgcgttc gggccgttct ctgttttggt tgtaaataag catttcctcc
     1801 ttgtacttaa taaaagcggg cgtcagtaga cacccgcgat aaaacgatta attcgtagca
     1861 tcaaccaatt tacctagtaa atctggtgag aagcgggtgc ttctactttc cacttttaga
     1921 cgatataaag atatcattaa tatgataatc tgtcaaattt aattgttatt ttaatgcatt
     1981 ttcgtataaa cgcgcgactt cgtcccaatt aatgacgtta aagaaagctt caacatattc
     2041 tggacgacgg ttttgatatt taaggtaata agcgtgttcc cacacatcaa gtgttaagat
     2101 tggggttcct tcatcaaagg gaatatcttg gtttggggtg ctaacgacct tgagtttttt
     2161 gtcactagta acgactagcc acgcccagcc agagccaaag cgtgattttg ctgcggtagc
     2221 aaaagcatct ttaaaagctt caaaagtgcc aaaggttgcc actagtgcat ctttaagttt
     2281 gccttctggt cctttgccgt tattaacttt gagtaaagtc cagaagaggt catggttata
     2341 gacaccgcct ccgttatttc taactgcggt tctaatgctt tctggtaatt tatctaagtt
     2401 tttaagtatt ttttctaaac tccaatcgtt aaacgatgga tctttcgcta gtgctgcatt
     2461 taaattagca acatatgtag cgtggtgttt tgaatggtga acttccatag tttttgcatc
     2521 gatataaggt tcaagtgcat cataagcata tggaagcggt tttaatgtaa atgccataat
     2581 ttttcctcct tttctctatt attatctcac agaagaagga gtttgtgttg cgttatgctt
     2641 tgtcacgaat ttcacgcttg ataagggtga taaattcacg ataaggtaca gtaatttgtg
     2701 ctttatcacc aaatttacga taagtaacta agttatcatt tcgctctttg tcaccaatga
     2761 caagcgtata aggaatttta ttaatttgac tattacgtaa acggtaacct aacttttcat
     2821 tactgtcgtc aagttcaaca cggagtcctt gtttttcaag tttgcgcaca acacttttag
     2881 cgtgttttaa atgggcatcg ttat
//
LOCUS       DAOPCD010000115         2883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_27293_length_2883_cov_3.554809, whole genome shotgun sequence.
ACCESSION   DAOPCD010000115 DAOPCD010000000
VERSION     DAOPCD010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27293_length_2883_cov_3.554809"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PLQ55_03465"
     CDS             <1..209
                     /locus_tag="PLQ55_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29023.1"
                     /translation="SLLSGPFARSLETNQKNSKTIFTVIPEAEKVTEVVKAVQDIISD
                     CKKQVVGFMFTLPVSGIYPLKPKK"
     gene            268..1788
                     /locus_tag="PLQ55_03470"
     CDS             268..1788
                     /locus_tag="PLQ55_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29024.1"
                     /translation="MRKRKINEFTEYLNHLNADDVQKVKAFMVNFKQHMNLPLSEEKK
                     LRKDLKGALMYYNAIGVSVTEALRRLDNENLGGFYARPPLLYYALDDAAKIYPLSMKH
                     GFQAVFRLSANLYDEVVPQLLQIALTFTIKRFPRFATTLKKGFFWHYLDANKQRYGIE
                     KEEHLPCRPIKVATTGSKSFRVLYYKNRISVEFFHSLTDGKGGIVFLQTLLDTYLRLF
                     GVEGHKHPSVLDINAAPLPEEDANEFKRTTVTKRSGFKEKRALQMSGKIESIRPTQIL
                     HFRLDAKELAKTAKSYDVTITTYVLALILLASRFATDAKEGDIAIQVPVDLRKYYESK
                     TLANFSLYGGIRIPITSITTMEQLLPLVKEQLVTATSHEKMSEMMYSAHRMNKDVRGF
                     PLFLKSPIAKLAYGLMGEAIFTTTLSNLGVVSAPDYFSDYIESYDFVLGPPEVNRAAL
                     TLITYNNIATLTITKSTKDPSFEEEIHRLLTKDGLDITIEGSAVYERKSRVSRKKY"
     gene            1751..2326
                     /locus_tag="PLQ55_03475"
     CDS             1751..2326
                     /locus_tag="PLQ55_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29025.1"
                     /translation="MNVKVVYPAKNIRHDIRRAAIYWTKPAFILAAIISAVVNILVKG
                     SAWSVIVIWSLWMIWSFVFTPTLIEHNRTSIAVKSSIHVTILIVIIYMIYPSWPGIEV
                     ASLVVVGGLIITAILFFSNVSRQKQNIFPLLIFIIIAIIFSVVAFVLRGQEPLGWAMI
                     VSISVALAIFLSTAIILRINFFRELKKRFML"
     gene            2344..2417
                     /locus_tag="PLQ55_03480"
     tRNA            2344..2417
                     /locus_tag="PLQ55_03480"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2376..2378,aa:Gly,seq:ccc)
     gene            2606..>2883
                     /locus_tag="PLQ55_03485"
     CDS             2606..>2883
                     /locus_tag="PLQ55_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29026.1"
                     /translation="MSNLVGYSIVALFVIVMGLLLLLKVYLQTYHPGKYWYIERPIKY
                     LMILGPMFFLFAIGERWHFGENFLPSQNPDDLAWGPFHLGWLFAMVIA"
BASE COUNT          908 a          528 c          532 g          915 t
ORIGIN      
        1 tctcattgct ctccggtcct tttgcgagat cacttgaaac taaccaaaaa aactcaaaaa
       61 caatctttac agttattcca gaagcagaaa aagtgacgga agttgtaaaa gccgtccaag
      121 acattatttc tgattgtaaa aaacaagttg tgggctttat gtttaccttg ccagtaagcg
      181 gtatttatcc acttaagcct aaaaaataaa atcatctaaa catcaataaa ttcacgatta
      241 cataatcgtg ttataattac tatcattatg agaaaacgca aaataaatga gtttaccgaa
      301 tatttaaatc acttaaatgc tgatgatgtc caaaaagtga aagcttttat ggtgaacttt
      361 aaacaacata tgaacttacc acttagcgaa gaaaagaaat tacggaaaga ccttaaaggg
      421 gctctgatgt attacaacgc tattggtgta tcagtaactg aagctctgcg ccgccttgat
      481 aatgaaaacc ttgggggatt ttacgcacgt ccccctcttt tatattacgc gcttgatgac
      541 gccgcaaaga tttatccttt atcaatgaaa cacggctttc aagctgtgtt tcgactatct
      601 gcaaatctat atgacgaagt tgtaccgcaa cttttacaaa ttgcgcttac cttcacgatt
      661 aaacgcttcc cgcgctttgc cacaacctta aaaaaaggtt tcttctggca ctatttagac
      721 gctaataagc aacgctacgg tattgaaaag gaagaacatt taccatgtcg tccgattaaa
      781 gtcgctacaa caggttcaaa atcattccgc gttttatatt ataaaaatcg gattagtgtt
      841 gaatttttcc attcgttaac agacggaaag ggcggaatcg tctttttaca aacactactt
      901 gatacttatt tacgtctttt tggtgttgaa ggacacaagc atccaagtgt actcgatatt
      961 aatgccgcac cacttcctga agaagatgca aacgaattta aaagaacaac tgtcacaaag
     1021 cgcagcggat ttaaagaaaa acgggcctta caaatgagtg gtaaaattga aagcattcgc
     1081 ccgacacaaa tattgcattt tagacttgat gcgaaagaac tagctaaaac tgccaagtca
     1141 tatgatgtca cgattacaac ttatgtctta gcgctaatcc ttttagcaag tcgctttgca
     1201 acagacgcta aggaaggtga tattgcaatt caagtacctg ttgatttacg caaatattat
     1261 gagtctaaaa ctctcgctaa tttttcgctt tatggtggga taagaattcc aattacaagt
     1321 attactacaa tggaacaact tttaccactt gtcaaggaac aattagtaac ggccacaagt
     1381 catgaaaaaa tgagtgagat gatgtattcc gcacaccgga tgaataaaga tgtccgcggt
     1441 ttcccgcttt tcttaaagtc accgattgct aaacttgcct atggactgat gggtgaagca
     1501 atctttacga caacattaag taatttaggg gttgtaagtg ccccagatta ttttagtgac
     1561 tatattgaaa gttatgactt tgtgcttggt ccacctgaag ttaatcgcgc agcattaaca
     1621 ttaatcactt ataataatat cgctacttta acaattacga aaagtacgaa agatccatcc
     1681 ttcgaggaag aaatacatcg tctgcttaca aaagatggtt tagacataac gatagaaggg
     1741 agtgctgtat atgaacgtaa aagtcgtgta tcccgcaaaa aatattagac atgacattcg
     1801 tcgtgccgct atttactgga caaagcctgc cttcatttta gcggcaatta tcagcgctgt
     1861 tgtcaatatc ttagtgaaag gctcggcctg gagcgtgatt gtcatatgga gcttatggat
     1921 gatatggtca tttgtgttta ctccaacact tattgaacac aatcgcacaa gtatcgccgt
     1981 gaagtcttcg attcatgtca ctattcttat cgtcataatt tatatgattt atccaagttg
     2041 gccgggaatc gaagttgctt cactcgtggt cgttggtggt ttaattatta ccgctatatt
     2101 attttttagt aatgtctcac gtcaaaagca aaatatcttt ccgcttttaa tctttattat
     2161 tatcgctatt atttttagtg tcgtggcctt tgtattacgc ggtcaagaac cacttggctg
     2221 ggcgatgatt gtttctatca gtgtcgcact tgctatattt ttaagtacgg caattatttt
     2281 aagaattaac tttttccgtg aactaaaaaa gcgatttatg ttatagttta ttgtagaaaa
     2341 aacgcagctg tagtttagtg gtagaacttt agcttcccaa gctaaccgtg cgggttcgat
     2401 tcccgtcagc tgctccatct agattgaaat tgcttcctaa agttgcatca aaaactactg
     2461 caacaaaaag ggagcttttt ttatttaatc atttttccca gcaaaaacgc attgttatta
     2521 cttcacgcaa acaaataaag ttttttcaat agaataaaaa agcaataagt tatataataa
     2581 cttcaatctg ccttaaggag agtgtatgtc aaatttagta ggttatagta tagtagcgtt
     2641 attcgttata gtaatgggtt tgttgctatt gcttaaagta tatttacaaa cttatcatcc
     2701 tggtaagtat tggtatatcg agcgaccaat aaaatattta atgattttag ggccaatgtt
     2761 ttttttgttt gcaattggtg aaagatggca ttttggtgaa aactttttac catcgcaaaa
     2821 tccagatgac ttagcgtggg gtccgtttca tcttggatgg ctctttgcca tggttattgc
     2881 aat
//
LOCUS       DAOPCD010000116         2841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_27831_length_2841_cov_2.656856, whole genome shotgun sequence.
ACCESSION   DAOPCD010000116 DAOPCD010000000
VERSION     DAOPCD010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27831_length_2841_cov_2.656856"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..1420
                     /locus_tag="PLQ55_03490"
     CDS             347..1420
                     /locus_tag="PLQ55_03490"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HPK29027.1"
                     /translation="MSNLQGFILLVLFVLNVGIALLLKLYLQTYEKGKYVIIERILKY
                     YMILTPMFFMFAIGERWRFGEKFLPSGQPDDLAWGPFHLFWLAAMVVGIIVASSRLKA
                     DKESNQRYLFGRLNAIDYTVFQFGILLVGIEFYKQMIFLDLYKGLAHYHWYGFPLQFC
                     SIPLILYPIVPFIKNEKIKEAFYSFIAIFNFVGGLSVMLLASGVYTLHVSISIHTMLW
                     HGTMVIAAFYLINAYKIGTKWRHYVGALTVLLALVIVAQLTNIAFHYIGQKYPGPDNF
                     DGFFISPWIDRKNMPVLGDIRVAMQESGLPVFLIAILFPHIYLVVFGFAGLLVFLIFR
                     AIWLDSERRHHAKEIAPAVSHTE"
     gene            1423..2013
                     /gene="rdgB"
                     /locus_tag="PLQ55_03495"
     CDS             1423..2013
                     /gene="rdgB"
                     /locus_tag="PLQ55_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPK29028.1"
                     /translation="MKKQIVIATNNANKLKEFKSLFENTPYEVLSLGDINFNGDIKED
                     EETFQDNAVIKATTIHKFTNLPVIADDSGLIINALDGFPGVNSARFMEGAPYSLKNAA
                     IIKMLEGHSDKSAKFVAAIALVGLEPFPQVFIGEVKGMITSEPKGEDGFGYDPIFFYP
                     HKQKTFAELTIKEKEEISHRGKATALLFEYLKEHHF"
     gene            complement(2010..2414)
                     /locus_tag="PLQ55_03500"
     CDS             complement(2010..2414)
                     /locus_tag="PLQ55_03500"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HPK29029.1"
                     /translation="MKQKEIKNVVAGLLFYNGKLFAASRSEKDERYGKFEFPGGKIEL
                     NESPALALKREIKEELAMDISVKDLFMHVSHEYPLFKLEMDVFICTVDKPSFKLLVHK
                     DGNFFCKDEILKLPFLGADLKVLAAIKERLDF"
     gene            2582..>2841
                     /gene="eno"
                     /locus_tag="PLQ55_03505"
     CDS             2582..>2841
                     /gene="eno"
                     /locus_tag="PLQ55_03505"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013059698.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPK29030.1"
                     /translation="MPRIIDVYAREVIDSRGNPTIEVDIQTESGAFGRAIVPSGASTG
                     EREALELRDGDKSRYLGKGVLTAVKNVNEIIAPKIIGMDTDD"
BASE COUNT          867 a          514 c          501 g          959 t
ORIGIN      
        1 ctgccttatt tagcgggatg ttcatctctg gcttattagg cacacttcta ccaatattct
       61 ttaccaaacg ggagatggac gctgacaatg cttcgggtcc aatcttaaca acgctagccg
      121 atataatcgc cattcttact tattatttaa ttgcctcaat aatgctaatt ttcttataag
      181 gattattttg taacctaact ctttttagtt aaaagatatc tttttttcct atgatttgcc
      241 cgcaaaaagg cattatttta taatgaacaa acacaaatta tggtctttta atagaattaa
      301 aaattgataa ggtatataat tatttaatgc gcattaagga gcatcaatgt ctaatctaca
      361 aggttttatc ttattagtac tattcgtact taacgtagga attgccctct tacttaaact
      421 ttatttacaa acttatgaaa aaggtaagta tgtaattatc gaacgtattt taaaatatta
      481 tatgattctt acgccgatgt tttttatgtt tgcaattggt gaacgttggc ggttcggcga
      541 aaaattttta ccatcgggtc aaccggatga cttagcgtgg ggaccatttc atcttttttg
      601 gcttgccgct atggtcgttg gtatcattgt ggcttcatcg cgtttaaaag ccgacaaaga
      661 aagtaaccag cgttatcttt ttggacgcct taatgccatt gattacaccg ttttccaatt
      721 tgggatttta cttgtcggga ttgaatttta taaacagatg attttcttag acctctataa
      781 aggacttgct cattatcatt ggtacggatt ccccttacaa ttttgttcaa tcccgctgat
      841 tctatatccg atcgtaccct ttattaaaaa tgaaaaaatc aaagaagcat tttattcttt
      901 tattgctata ttcaatttcg tgggcggcct cagtgttatg cttttagcta gtggtgtata
      961 cactctccat gtctcaattt caattcacac tatgctatgg catggaacga tggttattgc
     1021 cgctttttat ttaattaatg cttataaaat tggaacaaaa tggcgacatt atgttggggc
     1081 tctcactgtc ttattagcgc ttgttattgt agcgcaatta acgaacatcg ccttccatta
     1141 tattggccaa aagtatcctg gtcccgataa ctttgatggt ttctttattt cgccatggat
     1201 tgatcgcaag aatatgccag tcttaggtga tattagggta gcgatgcaag aaagcgggtt
     1261 accagtgttc ttaattgcca ttttatttcc ccatatttat ctcgttgtct ttggctttgc
     1321 tgggctatta gtgttcttaa tattccgcgc tatttggctt gattctgaaa gacgtcacca
     1381 tgcaaaggaa atagcgccag ctgtttccca tactgaataa ctatgaaaaa acaaattgtt
     1441 attgccacga ataatgcaaa taaacttaaa gagtttaagt ccctttttga aaatacacct
     1501 tatgaagttt taagtttggg agatattaat tttaacggcg atattaaaga agatgaggaa
     1561 acgtttcaag ataatgcggt gattaaagcc accactattc acaaatttac aaacttacca
     1621 gtgattgctg atgatagcgg actaataatc aatgcacttg atgggtttcc tggtgtcaat
     1681 tcggcgcgtt ttatggaagg ggcaccttac tctcttaaaa acgcggctat tattaagatg
     1741 cttgaaggcc atagtgataa aagtgcgaaa tttgtggccg ccatcgccct tgtcggtctt
     1801 gaaccattcc cccaagtttt tattggtgaa gttaaaggta tgattacaag tgaaccaaaa
     1861 ggggaagatg gttttggtta tgatccgatc ttcttttatc cccataagca aaaaacattc
     1921 gccgaattaa cgattaaaga aaaagaagaa ataagccatc gcgggaaagc aacggcttta
     1981 ctttttgaat atttgaaaga acatcatttt tagaaatcta aacgttcttt aattgccgct
     2041 agtactttta aatccgcccc taaaaagggc aattttaata tttcgtcctt acaaaagaaa
     2101 tttccgtctt tatgaactaa taacttaaaa ctaggtttat caacagtaca aataaataca
     2161 tccatctcta atttaaacaa aggatattcg tgactaacat gcataaataa atctttaaca
     2221 cttatatcca tcgctaattc ttctttgatt tcacgcttta aggctaaggc cgggctctca
     2281 tttagttcaa ttttaccacc tggaaattca aatttcccat agcgttcatc tttctcactg
     2341 cgtgaagcgg caaaaagttt tccgttataa aataataatc ccgccacaac atttttaatt
     2401 tctttttgtt tcataatcat aaactctata aattagtata acaaactagc atatttttac
     2461 gcggtaaatt gatatcggga taaagttact gataaatact tatatttagg actttttact
     2521 acaacccagc cctaaaaaag tttataatat atgtggctaa ttacaaagag gaggaaaaaa
     2581 catgccaaga attattgatg tatacgctcg tgaagtcatc gactcccgtg gtaatccaac
     2641 cattgaagtt gatattcaaa ctgaatctgg ggcctttgga cgtgccattg ttccaagtgg
     2701 cgctagtact ggtgaacgcg aagcattaga attacgcgat ggcgataaaa gtcgttatct
     2761 cggcaaagga gttttaactg cggttaaaaa tgttaacgaa attattgctc caaaaattat
     2821 cggaatggat accgatgacc a
//
LOCUS       DAOPCD010000117         2815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_28158_length_2815_cov_3.663768, whole genome shotgun sequence.
ACCESSION   DAOPCD010000117 DAOPCD010000000
VERSION     DAOPCD010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28158_length_2815_cov_3.663768"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PLQ55_03510"
     CDS             <1..1044
                     /locus_tag="PLQ55_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK29031.1"
                     /translation="SYTIVAFVMALGGYFALQGLVTMPILFMFIRAAMNTFEPILNLT
                     NFINELQVAQASAERVLQLVNEKPSIDDTQEVKDVYGDLFDKKKENWEEIKGDVEFKD
                     VTFYYNPKEIILDKFNLKVKAGQTVALVGHTGSGKTTIVNLFARFYEPRAGEILIDGK
                     DYRKRSLSWLHSRLGYVLQMPELFSTSIKENIRYGNLEASDEEVIAASKAVGLDEFVN
                     TLEKGYDTHVGEAGNLLSVGQKQLISFARAIIADPRILILDEATSSIDSEAEAKIQKA
                     TDELLKGRTSFVVAHRLSTIVNSDLIVVLENGKIIEQGTHAELLGNRGHYFELYRNQF
                     IAERREEKYLEID"
     gene            1047..1250
                     /locus_tag="PLQ55_03515"
     CDS             1047..1250
                     /locus_tag="PLQ55_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HPK29032.1"
                     /translation="MTRTYIVEGMKNERSVRRIERALFRVDNISYEINLEKNTVVIHF
                     SNRVDDDKVIGAISRAGYRAMRL"
     gene            1397..1519
                     /locus_tag="PLQ55_03520"
     CDS             1397..1519
                     /locus_tag="PLQ55_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29033.1"
                     /translation="MVLGSEKKYLCLLCGMIIDNPDYCEYCGATSENIVPYEEE"
     gene            1654..>2815
                     /locus_tag="PLQ55_03525"
     CDS             1654..>2815
                     /locus_tag="PLQ55_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPK29034.1"
                     /translation="MEENRPLFYGEVHVEGNRITYVGPSGDFNEPFDRIINAEGNLLM
                     PGFKNAHTHSPMTFLRSYADDLPLYDWLHKAVFPLEDKLEPGDQYVLSKIAFAEYLTS
                     GVTACFDMYYSPLETAQAAIDVGFRAALVGTVTKYKESVCEMKAAYHTINKMHPLVSY
                     HLGFHAEYTSTPEILKELAKAAHELKVPIWSHISETETEVKECIERHGYTPAVYFDKL
                     GLYNYGGGGFHGVHFSDEDFAVYKKRGLSIVTNPSSNTKLASGIAPINKFLKEGINVA
                     IGTDGPASNNALDFFREMWLVTGLAKLREKDAAVVDAYDVLKMATVNGAEAMGLKDAK
                     YLSVGSLADIIMLDMQQPNMQPVNNIEKNIVYAGSKVNVLLTMIGGKILYEDG"
BASE COUNT          937 a          430 c          604 g          844 t
ORIGIN      
        1 agttacacaa ttgttgcctt tgtcatggcg cttggtgggt actttgccct ccaaggctta
       61 gtgacgatgc cgattctctt tatgtttatt cgtgcggcaa tgaatacttt tgaacctatc
      121 ttaaacttaa cgaactttat taatgaacta caagtcgcgc aagctagtgc tgaacgtgtt
      181 ttacaattag tcaacgaaaa gccgtcgatt gatgatacgc aagaagttaa agatgtgtat
      241 ggtgatttat ttgataagaa aaaagaaaat tgggaagaaa ttaaaggtga tgttgaattt
      301 aaagatgtga cattctatta caatccaaaa gaaattattc tcgataaatt taatttaaaa
      361 gttaaagctg ggcaaacagt agcacttgtt ggtcataccg gcagcgggaa aacaacgatt
      421 gttaatcttt tcgcaagatt ctacgagccg cgcgctggtg aaatattaat tgatggtaaa
      481 gattatcgaa agcgtagtct atcatggctc catagccgct taggatatgt tttacaaatg
      541 cccgagcttt tctccacatc gattaaagaa aacattcgct atggtaattt agaggcaagt
      601 gatgaagaag tcattgctgc gagtaaagca gtcggccttg atgaatttgt gaatacctta
      661 gaaaagggat acgacacgca tgttggtgaa gctggtaacc ttttatcagt gggccaaaag
      721 caactcatct cttttgcgcg agcaattatt gctgatccac gaattttaat tcttgacgaa
      781 gcgactagtt cgattgatag cgaagctgaa gcaaaaatcc aaaaagccac tgatgaactc
      841 ttaaaaggcc gaacaagttt tgttgttgcg caccgcttaa gcacgattgt taatagtgat
      901 ttaatcgttg ttttagaaaa cggtaagatt attgagcaag gtactcacgc tgaattacta
      961 ggtaatcgcg ggcattactt tgaattatat cgaaatcaat ttatcgcgga acgccgtgaa
     1021 gaaaaatatc tggagattga ttaatagtga cacggactta tattgttgaa gggatgaaaa
     1081 acgaacgatc cgtacgtcgt attgaacgcg cgttatttag agtggataat ataagttacg
     1141 aaataaattt agaaaaaaat actgttgtga tccactttag taatagagta gatgatgaca
     1201 aagttatcgg tgcaattagt cgcgctgggt atcgggcaat gcgattataa ttaatgaaaa
     1261 tttaaaattt tgtaataaaa aacttgattt aaacacattt ataaataaag aaaattagtt
     1321 gtaaattaat gcatcagcgg gtttacttct aataattata agatataata gggtatggga
     1381 ggaagaggaa ataaatatgg tattaggttc tgaaaagaaa tatttatgtt tattatgtgg
     1441 gatgattatt gataacccag attattgtga atactgtggt gcaacatcag aaaacatcgt
     1501 tccttacgaa gaagaataaa tattaaataa acatataaaa aagcatgggg caacccatgt
     1561 tttttattat taataaaaac atgctaaaat taagtatcta ataaatgagg aaaacattat
     1621 gaaaatacat ttattaacgc acttattcta acaatggaag aaaatcgtcc gcttttttat
     1681 ggtgaagtcc atgttgaagg taatagaata acttatgttg ggccaagtgg tgactttaat
     1741 gaaccgtttg accgtattat taacgctgaa ggtaatttac tcatgcctgg ctttaaaaac
     1801 gcccatacgc attcaccgat gacattttta cggagttacg ctgatgactt accgctttat
     1861 gactggttac ataaggccgt atttccgctt gaagataaac ttgagcctgg cgatcaatat
     1921 gtcttaagta aaattgcttt tgcggaatac ttaacaagtg gcgtgacggc atgttttgat
     1981 atgtattatt caccacttga aacagcgcaa gcggcaattg atgttggttt tagagcggca
     2041 cttgtgggca ctgttacaaa atacaaagaa agtgtttgcg aaatgaaagc agcatatcac
     2101 acgattaata aaatgcatcc gcttgttagt tatcatcttg ggttccacgc tgaatataca
     2161 agcacgcccg aaatattaaa agaattagcg aaagctgctc acgaattaaa agtcccaatt
     2221 tggagtcata ttagtgaaac agaaacggaa gtaaaagaat gtattgaacg ccacggctac
     2281 accccagcag tgtattttga taaattaggt ttatataatt acggtggtgg cggtttccac
     2341 ggagtgcact ttagtgatga agactttgcc gtgtataaaa agcgcggttt aagtattgtg
     2401 accaatccta gttcaaatac taaacttgca agcggaattg ccccgattaa caaattcctt
     2461 aaagaaggga ttaatgttgc aattggcact gatgggcccg ctagtaataa cgcgcttgat
     2521 ttcttccgtg aaatgtggct tgttactggt ttagcgaagt tacgtgaaaa agacgcggcg
     2581 gttgttgatg cttatgatgt ccttaaaatg gctactgtaa atggtgctga agcaatggga
     2641 ttaaaagacg ctaaatattt aagtgttggc agtctagcgg acatcattat gcttgatatg
     2701 cagcaaccga atatgcaacc tgttaataat attgaaaaaa atattgtgta cgctggtagt
     2761 aaggttaatg tattattaac gatgattggt ggcaagattt tatatgaaga cggtg
//
LOCUS       DAOPCD010000118         2804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_28318_length_2804_cov_3.213532, whole genome shotgun sequence.
ACCESSION   DAOPCD010000118 DAOPCD010000000
VERSION     DAOPCD010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28318_length_2804_cov_3.213532"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            401..856
                     /locus_tag="PLQ55_03530"
     CDS             401..856
                     /locus_tag="PLQ55_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29035.1"
                     /translation="MDLNLLQPKVEELLKERGYRLVSMRFLNEEGTRFLRVIVDKYQH
                     IISLDEIVAISEAIDAILDDDEESAFTLDITTTGAEKEIALQEVNDYLGLFIKLTMKD
                     GVKGPNTVKGILEAVSENEVTISHNDKGRIKKETYAQSDIKTINRAVKC"
     gene            871..2538
                     /gene="nusA"
                     /locus_tag="PLQ55_03535"
     CDS             871..2538
                     /gene="nusA"
                     /locus_tag="PLQ55_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPK29036.1"
                     /translation="MSKKLTKAQLRKKQERFEAMREMFREVAEKHNLKEEVVLNVFMR
                     AVQNVVARQKGGDDVHVEVVINEEMFDFEARVLKRVVAHDDDIEDDALEVCLEVARTF
                     DADAEVDALVVVERSFLGDFNSKLSNNVAHNFRHQLIELEKEQLFTLFKDKIGELVKG
                     TVEKNERITYINLLGITSVFFDERGRHAIPGEVFKPGQELNFYIVDVVSGAKGANIII
                     SRSHPNFVKRLMEREIHEIYDGTIVIHDIVREEGERVKVAVESLDPNVDPIGACIGPN
                     ASRIQKITAELGPDGRKEKIDIIPWVKNEALFIIEAFRPAVPLGLKIDEENKTAQVVF
                     SEEEKGKAFGHKGANIRLASKLTGYDLTILDLNEASEQGVEFLTKADIEYEERLAEQE
                     RTRERILRELARAEEVAQRAASQKSLLEEEVNSLNIDDLEYEDAFKDEEKVVETPKVV
                     TPEIKEEKKEPVVQKVKTTISLADLEAELAKEKEEIEQAQQKREERRSRTKQEVKQPE
                     VVEPTLPTGPKMDIYTEEELAELDAELEEVYEDDDLDFEDFDEYYDN"
     gene            2549..>2804
                     /locus_tag="PLQ55_03540"
     CDS             2549..>2804
                     /locus_tag="PLQ55_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HPK29037.1"
                     /translation="MKKIIVRRCIVTHESHEKRDLIRVVRTPDGRVVIDNTGKVNGRG
                     AYLVRNKEVILLAQKKNALSQALNIEVDQVIYEKLLEEVND"
BASE COUNT         1020 a          432 c          590 g          762 t
ORIGIN      
        1 taaagataat atttttgatg gttttgaaaa agtatataaa ttaagtgtaa atgaattaga
       61 aatgaagttt aaaaaagaaa ttgctaaact tgaactaaca aatgatactg aaagtaaaat
      121 taaagaatta ttaatgaaaa acaaaacatg aaaatcaaaa cagtactggt taagtaagtc
      181 ttttttacta ttgcgacggt attggatcgt gtacttctta agaaaaaatc attgtaaatt
      241 taaggtgtaa atatgaaata atcgctaata ttatagtaat ttgacacttt ttagttgcaa
      301 agttgtacaa aatctactat tatgatactg taagttttac ttacaaatta attcaaactc
      361 tagagagtgg gtacgcccac tctttgttta caggaggatt atggatttaa acttattaca
      421 accaaaagtc gaagaactat taaaggaacg cggttatcgc ttagtaagta tgcgtttctt
      481 gaatgaagaa ggtacacgtt tcttacgcgt aatcgttgat aagtaccagc acattattag
      541 tcttgatgaa attgtcgcca tcagtgaagc aattgacgca atactagatg atgatgaaga
      601 aagtgccttc acccttgata ttacaacgac gggcgcggaa aaagaaatag cgctacaaga
      661 agtaaatgat tatctaggtt tattcattaa attaacgatg aaagatggtg tcaaaggtcc
      721 taacacagta aaaggtatcc tagaagctgt tagtgaaaat gaagtgacga ttagtcataa
      781 cgataaagga cgaattaaaa aggaaactta tgcgcaaagt gatattaaaa caattaatcg
      841 cgcagtgaaa tgttaaagag gaatacgaaa atgagtaaaa aattaacaaa agcacaatta
      901 agaaaaaagc aagaacgttt cgaagcaatg cgtgaaatgt ttcgtgaagt tgcggaaaag
      961 cacaacttaa aggaagaagt tgttcttaat gtttttatgc gtgctgtcca aaatgttgtc
     1021 gctcgccaaa agggtggaga tgatgtgcat gttgaagtcg tgatcaacga agaaatgttt
     1081 gattttgaag cacgggtctt aaaaagagtt gtcgctcatg atgacgatat tgaagatgat
     1141 gcacttgaag tatgtttaga agtagcgcgt acatttgacg ctgatgcaga agtcgatgca
     1201 ttagtcgtcg ttgaacgctc attcttaggc gatttcaatt caaaattatc aaataatgtc
     1261 gcccataact tccgtcatca attaattgaa cttgaaaaag aacaactctt cactttattt
     1321 aaagataaaa ttggtgaact tgttaaaggt accgtggaga aaaatgaacg aattacttat
     1381 attaatttat taggaattac aagtgtgttc tttgatgaga gaggacggca cgcgattcct
     1441 ggtgaagttt ttaaacctgg ccaagaatta aacttctata ttgttgacgt cgttagtggc
     1501 gctaaagggg caaatattat tatttcccgc agccatccta acttcgtaaa aaggctcatg
     1561 gaacgcgaaa ttcatgaaat ttatgatggt actatcgtta ttcatgatat cgtgcgggaa
     1621 gaaggtgaac gcgtcaaagt tgcagttgaa agtcttgatc caaatgttga tccaattggc
     1681 gcctgtattg gtccaaatgc aagccgcatt caaaaaatta ctgctgaact tggtcctgat
     1741 ggccgtaaag aaaagattga tattatccca tgggttaaaa acgaagcgct cttcattatt
     1801 gaagccttcc gtccggctgt gccgcttggc ttaaaaattg acgaagaaaa taaaacagca
     1861 caagtcgtct ttagcgaaga agaaaaaggt aaagccttcg gtcataaagg cgcgaatatt
     1921 cgtttagcga gtaaattaac tggctacgac ctaacgattc ttgatttgaa tgaagctagt
     1981 gaacaaggtg ttgaattctt aacaaaagcg gatattgaat acgaagaacg gctcgctgaa
     2041 caagaacgga cacgcgaacg tattttacgc gaacttgcgc gtgccgaaga agttgcacaa
     2101 cgcgccgcta gtcaaaagag tttgcttgaa gaggaagtta attcccttaa tattgatgac
     2161 ttagaatatg aagatgcttt caaagatgaa gaaaaagtcg ttgaaacacc aaaagtcgtc
     2221 actcctgaaa ttaaagaaga aaagaaagaa ccagttgtgc aaaaagttaa aacaacaatt
     2281 tcgcttgctg acttagaagc agaacttgcc aaagaaaaag aagaaattga acaagcacaa
     2341 caaaaacgcg aagaacgtcg tagtcgcact aaacaagaag ttaagcaacc tgaagttgtt
     2401 gaaccaacac ttccgactgg accaaagatg gatatttata cggaagaaga acttgcagaa
     2461 ctcgacgctg aacttgaaga agtctatgaa gacgatgatc tcgactttga agatttcgat
     2521 gaatactacg ataactagga gtgtgtaaat gaagaaaatt atcgttcgcc gctgtattgt
     2581 tacccatgaa agtcacgaaa aacgtgatct tattcgtgtt gtaagaacgc cagacggacg
     2641 tgtggtaatt gataatacgg gaaaagtcaa tggtcgcggt gcttacttag tgcgcaataa
     2701 agaagttatt ctcttagcgc aaaagaaaaa tgccttaagc caagccttaa acattgaagt
     2761 tgaccaagtt atatacgaaa aactccttga ggaagttaat gact
//
LOCUS       DAOPCD010000119         2773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_28716_length_2773_cov_4.597498, whole genome shotgun sequence.
ACCESSION   DAOPCD010000119 DAOPCD010000000
VERSION     DAOPCD010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28716_length_2773_cov_4.597498"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..1191)
                     /locus_tag="PLQ55_03545"
     CDS             complement(226..1191)
                     /locus_tag="PLQ55_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPK29038.1"
                     /translation="MNKLEKILTIVASSLLVVGLVIFISVLAVNGFNLGGLSTVKYEA
                     VTHEVSEDYAKIQFDISVGDVTIVKGEEATTKAICYENKRLTYNLNVEEDTLVVTDNN
                     TMRRFWTIFDLWGNKGITLYVPHDTYTSLLVKNNTGTLNVSSSVTFNTITARLETGSI
                     TLNGDASVVDLSSETGSITLNNVDADSARLVTATGRHVLNNVNVTGNLNIEVDTGTVK
                     LTNVKSDNLTVDSDTGTITLKDTVVTNTMRLAASTGSINFDKSDAHDIYARTSTGSIK
                     GSLLTGKVFSTKTNTGTISVPSNTAGAGVCDLRTDTGVIKITIAA"
     gene            complement(1188..1478)
                     /locus_tag="PLQ55_03550"
     CDS             complement(1188..1478)
                     /locus_tag="PLQ55_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29039.1"
                     /translation="MLIGAIAVFFSLVISLWAVDLGLFVGSFEAFITVFTLLFAGEGL
                     KALAYFGISLAMAGLGILLLLVGVKLTKYTIELAKKLINKIKTKMVLKGAKK"
     gene            complement(1662..1790)
                     /locus_tag="PLQ55_03555"
     CDS             complement(1662..1790)
                     /locus_tag="PLQ55_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29040.1"
                     /translation="MTKKEFLSALREKMAGLPKDDVDDRIAFYSEMIDDRMEEGLS"
     gene            complement(1783..2103)
                     /locus_tag="PLQ55_03560"
     CDS             complement(1783..2103)
                     /locus_tag="PLQ55_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008402371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPK29041.1"
                     /translation="MDVQLKRGLLEVCVLAVIRNKPTYGYRIIKDMKPYVEISESTLY
                     TILKRLEENEMLTVKTVEHNSRLRKYYSITGRGLGRIAEFKEYWKDMEAMYKFIIKKE
                     SSDD"
     gene            complement(2246..2752)
                     /locus_tag="PLQ55_03565"
     CDS             complement(2246..2752)
                     /locus_tag="PLQ55_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29042.1"
                     /translation="METKNINTKQLSIIVRIGIIFLFIIGLIMVIVWYPFTISLTIIG
                     PVDVEPTQLQKIQVYAQSAFYYLTSVPCFIILIFLWIIAGRLKKNGRFIAKDVRLLNA
                     ANIMLLIDLAIFLVGNIIIVFLEINFFVLAHFFIAVIGLVVLIFMWTTTLLIRRNVEL
                     QALIETDS"
BASE COUNT          919 a          571 c          394 g          889 t
ORIGIN      
        1 atttggtcgc tttgttttta caagccgcac atcactagcg ccgttttcaa tataaagatc
       61 ttcaaaatat tcatattcat ccaaaataaa ttctactttg ttagtacttt gtttcatttt
      121 aagagactcc tatttttcta attttatcac gcttaatgta acaatacttt taaataagaa
      181 aaagtgcgtt acttctaacg cactcactcg ggggacaaaa tttaactatg ccgcaatcgt
      241 aatcttaatc actccagtgt cagtgcgaag atcacaaact ccggcaccag cggtattact
      301 tggcacacta atggtaccag tatttgtctt agtgctaaag actttaccag ttaagagtga
      361 acctttaata ctacctgttg aagttctagc gtaaatgtca tgggcatcac ttttatcaaa
      421 gttaatggag cccgtactag cggctaaacg cattgtattt gtcacaactg tatcttttaa
      481 agtaatcgta ccagtatcac tatcaactgt taaattatca cttttaacat tcgttaactt
      541 aactgtgcca gtatcaactt caatatttag gtttcctgtt acattaacat tatttaagac
      601 gtgtcttcca gttgccgtca ctaatctagc gctatctgcg tcaacattat ttaaagtgat
      661 tgagcctgtt tccgagctaa gatctacgac acttgcatca ccatttagtg tgatcgaccc
      721 cgtttctaat cgtgccgtaa tcgtgttaaa agttacgctg cttgaaacat ttaatgtccc
      781 cgtattattt ttcactaaaa gtgaagtata agtatcatgc ggaacataaa gcgtaatacc
      841 tttattaccc cataaatcaa agatagtcca gaaacgacgc atcgtattat tgtcagtaac
      901 aacaagcgtg tcttcttcaa cgttaaggtt ataagttaag cgtttatttt cataacaaat
      961 cgctttagtc gttgcttctt cacctttaac aatcgtgaca tccccaacac tgatatcaaa
     1021 ttgaatcttt gcataatctt cactaacttc atgagttaca gcttcatatt tcaccgtact
     1081 taatcctcct aaattaaagc cgtttaccgc taaaacacta ataaatatga ctaggccaac
     1141 tacgagcaag gaactcgcaa ctatcgttaa aatcttttct aacttattca tttttttgct
     1201 cccttcaaca ccatctttgt tttaattttg ttaataagct ttttcgctaa ctcaattgtg
     1261 tacttcgtta acttaacacc cactagtaat aaaaggatcc ctagtcccgc cattgcaaga
     1321 cttatcccga aataggctag tgctttaagt ccttcaccag caaaaagcaa agtaaagaca
     1381 gtaataaacg cttcaaatga gccgacaaaa agaccaaggt ctacggccca gagcgaaatt
     1441 acaagcgaga aaaacaccgc aattgccccg ataagcaaac cgcctaatcc aataagaagc
     1501 ggtacccaaa gcggtgcaag tacaataatt agaacaattt cccatccttc taactttcgt
     1561 tttggtgtta ctttgtcttt aacaatctta gtaattgatg tgccttcaat ttgtttaatg
     1621 acgatctcat caacattacc aacttcggcg accgcttctg cttaacttag tccttcttcc
     1681 atgcggtcat caatcatttc gctataaaaa gcaatacgat cgtccacatc gtcttttggt
     1741 aatcctgcca tcttttcacg caaggcactt aaaaactcct ttttagtcat cactactctc
     1801 cttcttaata ataaatttgt acatcgcttc catgtctttc cagtattctt taaattcagc
     1861 aatgcggcca agccctctcc ctgtaatact gtaatatttc cggaggcgag aattgtgttc
     1921 aactgtctta acagttaaca tctcattttc ttcgagacgc tttaatattg tgtataatgt
     1981 cgattcagat atttcaacat atggcttcat atctttaata atacggtagc cataagttgg
     2041 tttattacga atgacagcaa gtacacaaac ttctaagagt cctcgtttta gttgtacatc
     2101 caaagtctta tacctccctt aacataatac atcgtaagcc gaagtatata aaagtcaatt
     2161 catattatta aaaatttata ttttctttta aattgaaaaa agagtagtta taaactacta
     2221 aatttaaaaa tgattaagga cattattaag aatcagtttc aatcagtgct tgtaattcaa
     2281 cattacgcct aataagtaat gttgttgtcc acataaaaat taatacgaca agcccaatta
     2341 cagcaataaa gaaatgcgca agtacaaaga aattaatttc taagaaaaca ataataatat
     2401 taccaactaa aaatatcgct aaatcaatta ataacataat attagcagcg ttaagcaaac
     2461 gcacatcttt agcgataaac cgaccatttt tctttaagcg accagcgatt atccacaaaa
     2521 atattaaaat gataaaacat ggcactgatg taagataata aaacgctgat tgcgcatata
     2581 cttgaatctt ttgtaactgt gttggttcaa catcaactgg gccgataatt gtaagcgaaa
     2641 tagtaaaagg ataccagaca ataaccataa ttaagccgat gataaaaaga aaaataatac
     2701 caatgcggac aatgatcgat aattgtttcg tatttatgtt tttagtttcc ataacataat
     2761 tataacgcta aaa
//
LOCUS       DAOPCD010000120         2771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_28750_length_2771_cov_2.879602, whole genome shotgun sequence.
ACCESSION   DAOPCD010000120 DAOPCD010000000
VERSION     DAOPCD010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28750_length_2771_cov_2.879602"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PLQ55_03570"
     CDS             <1..268
                     /locus_tag="PLQ55_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019909459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29043.1"
                     /translation="TLTLESIAQGEEFTKTGDLKYRESKMVSNFIREFDALDNEVIMG
                     IYGSAHISKDFIAFFLGIEPMTIQLKKYYEPQNILIYAKELTRL"
     gene            348..424
                     /locus_tag="PLQ55_03575"
     tRNA            348..424
                     /locus_tag="PLQ55_03575"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:382..384,aa:Arg,seq:tct)
     gene            612..1361
                     /locus_tag="PLQ55_03580"
     CDS             612..1361
                     /locus_tag="PLQ55_03580"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HPK29044.1"
                     /translation="MKDLATLVKEFKTIIDNSNNVVFFGGAGVSVPSGIPDFRSADGV
                     YSKDTNTMYSAEEMVSRTMYYRNTPFFNDFYFKYLVYPDALPNPCHTYLARLEKEGKL
                     KAVVTQNIDGLHQAAGSKVVYELHGSVHRNYCTLCKAFHDLAATLKEPRGKCKKCGGY
                     LKPDVVLFEEGLDQNTITRSIEAIAKADTLIIGGTSLVVQPAASFINFFRGKNIVVVN
                     KTSIRLPMYLSTNILFIEADIGKTFELLNKL"
     gene            1413..1568
                     /locus_tag="PLQ55_03585"
     CDS             1413..1568
                     /locus_tag="PLQ55_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29045.1"
                     /translation="MLNKVINDIASLVEVSYDKSTTIALVLFIGVMGLTIITLYYFLT
                     KNNKKKK"
     gene            1794..>2771
                     /locus_tag="PLQ55_03590"
     CDS             1794..>2771
                     /locus_tag="PLQ55_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29046.1"
                     /translation="MRKIIKKALGPLAIFAMALIVGLSVAKEKTPVGVKAAPVYTITE
                     DFETQTALTASYADGNFVGAGGITFYYGHSRDQDKYPIDGKGIMLRRASDSYLEFQLK
                     KGLSGLKFKYRKGFTGPGARQLELLVNNTQVSLTAEFGADSGALATIFDFVYEPDVSH
                     SGLINVKIKVPGTADSNKHVILDNIQIAENSASTDVITALSVTPTSKTYYTTDTLQET
                     DFAVNLTKNGATAPGSSANYDYFVKIGTGTGTTFVGEYISWGVTQPLTTHTNIQFKAC
                     LPQTAGNTDFIIVDIPITVSVPTLSSIAITGNMTKTSYFVGGVWNPAGLT"
BASE COUNT          941 a          443 c          495 g          892 t
ORIGIN      
        1 cactttaaca cttgaatcaa tcgctcaagg cgaagaattt actaaaactg gcgacttaaa
       61 atatcgtgaa agtaagatgg taagtaactt tattcgtgaa tttgatgctt tagataatga
      121 agtgataatg gggatatatg gtagtgcaca tattagtaaa gactttattg ctttcttttt
      181 aggcattgaa ccaatgacaa ttcaacttaa aaagtattac gaacctcaaa atatattaat
      241 atacgctaaa gaactcacac gactttaaaa gctaaattta acaataaaac tattttttaa
      301 tgtcacttgt tgaaactaat attcttttat tgtaaaataa cttgcgtgtc ccggtagctc
      361 agtcggatag agcaacagcc ttctaagctg tgggtcgggc gttcgagtcg ctcccgggac
      421 gccattaatt aatgaagaag agttagtgtt taccccctag atgctgactc tttttttcta
      481 acattaaatt gaactagttt tgtgataaaa acataacata aaggctaatg gtaattaatt
      541 agtaaataat tagtagaata tttagtagag aaagcattaa atagcttatt cttaagtgtt
      601 ataataaaag tatgaaagat ttagcaacat tagtgaaaga gtttaagact attattgata
      661 atagtaataa tgttgtcttt tttggtggcg ctggtgtgag tgtcccgagc ggtattcctg
      721 attttagaag tgccgacgga gtgtatagta aagacacgaa tacaatgtac tcagcagaag
      781 aaatggtgtc acgcacgatg tattatcgta atactccttt ctttaacgac ttttatttta
      841 agtatcttgt ctatcccgat gcgcttccta atccgtgcca cacttattta gctagacttg
      901 aaaaagaagg taaattaaaa gcagtagtga cacaaaatat tgacggttta catcaagccg
      961 ctggttcaaa agtcgtctat gaacttcacg gcagtgtgca ccgtaattac tgtactttat
     1021 gcaaagcatt tcatgattta gcggcaacac taaaagagcc gcgtggtaaa tgtaaaaagt
     1081 gcggcggtta tcttaaacct gatgttgtgt tatttgaaga aggactagat caaaacacga
     1141 ttacgcgttc aattgaagca atagcaaaag ctgatacttt aatcatcggt ggcactagtt
     1201 tagtcgtgca acccgctgcg agttttatta atttctttcg gggtaaaaac atcgttgttg
     1261 tcaataaaac aagtattaga ttaccaatgt acttaagcac taatatactt tttattgaag
     1321 ctgatattgg taagaccttt gaattactaa acaagttata aaaagcaatc gtataatcgt
     1381 gctattatat taagcaagag aaagaatcat aaatgttaaa caaagttatt aatgatattg
     1441 cttccctcgt tgaagtaagt tacgacaaaa gcaccactat tgccttagtg ttatttattg
     1501 gtgtaatggg tttaacgatt attacacttt actatttcct aacaaagaac aataaaaaga
     1561 agaagtaaca cttctttatc aataattatg taaacaaaaa aattaggtgt ttattattaa
     1621 acacttaacc acttttttac aaataaaaat tatgaactta tcatcaaagt tttaatgctt
     1681 tcttaataat tcattattaa ttcgctcctt gcaaagtttt attattttgt ctaatatgta
     1741 tactagatat atatgtgcgt aatatcctaa aaaaataata aagaggaaat aatatgcgca
     1801 aaataattaa aaaggcatta ggaccattag ccatttttgc aatggcacta attgtcggtc
     1861 ttagtgtggc aaaagaaaag acacctgtag gggtgaaagc tgctccagtt tacaccatta
     1921 ctgaggactt tgaaacgcaa acagctttaa cggcatcata tgcggatgga aattttgttg
     1981 gggcaggtgg aattactttc tactatgggc attcgcgaga tcaggataaa tatcctattg
     2041 atgggaaggg aattatgcta cgacgagcaa gtgattctta cctagaattc caattaaaaa
     2101 aaggactctc tggtcttaaa ttcaaatata gaaaaggatt tactggtcca ggcgctcgcc
     2161 aattagaatt gcttgttaat aatacacagg tttctcttac tgcagagttt ggtgctgatt
     2221 caggtgctct tgcaactatt tttgattttg tctatgaacc agatgtatca cactctggtc
     2281 taattaatgt gaaaattaaa gttcctggta ccgcagattc aaacaaacat gttatacttg
     2341 ataatattca aattgcagag aattctgcat ctacagatgt tattacagca ttaagcgtaa
     2401 caccaacatc aaaaacatat tatacaactg ataccttaca agaaactgat tttgcagtga
     2461 atcttactaa aaatggtgct actgccccag gaagtagtgc taattatgac tattttgtaa
     2521 aaattggtac tggtacaggc acgacttttg ttggtgaata tatttcttgg ggagttactc
     2581 aaccgttaac aacccataca aacattcagt ttaaagcttg cttaccacaa actgctggaa
     2641 atacagactt tattatcgta gatataccaa ttacagtttc tgtaccaaca ctatcttcaa
     2701 ttgcgattac tggtaacatg accaaaacat cttattttgt tggtggtgtt tggaatcccg
     2761 ctggtttaac t
//
LOCUS       DAOPCD010000121         2762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_28858_length_2762_cov_3.621352, whole genome shotgun sequence.
ACCESSION   DAOPCD010000121 DAOPCD010000000
VERSION     DAOPCD010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28858_length_2762_cov_3.621352"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1546)
                     /locus_tag="PLQ55_03595"
     CDS             complement(158..1546)
                     /locus_tag="PLQ55_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29047.1"
                     /translation="MKDKIRESKLALISLFLALEVVLFIALDFGNIGPVFKALAFLLT
                     AILAPFFFEEMKVDLGQGLYVLLMPILFYALVTLVAPAYGSYPEMYSNEVFMQLPFIT
                     RLINLLGIVGLLLLGYFIGKSEVFATKHIFIVILAGLAAPVLISLFATLINYGFFHPL
                     VYKSLVTYYAGHEYEVSSQASLLYGFRIMVGDLKALASMAVIITSAGFGLLFVNEKKD
                     KFTIISLVVISAVGVLTLIFTASFAALIYLLPALIFALLLKFDLLKKLKNKVTLGVVI
                     ALVGLGAIIFLLTAYNIFNLQAVWQTNRVTRKLFFNGYMQRFYIIFKEAFGFRNLYGH
                     FAMTVEGYKVFPSGNFLFDAMWIDGLIGFVALLAFFVMFVIRLVKYFNKDKDERVLKV
                     VLISVLLTAFFRFMLRYPFKIFTYQEFSDINNFPLINSPLFLVTVFIAGYVFVLKEDK
                     KIEEEEVTHAEK"
     gene            complement(1546..2487)
                     /locus_tag="PLQ55_03600"
     CDS             complement(1546..2487)
                     /locus_tag="PLQ55_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29048.1"
                     /translation="MKRTRRSYIWEFLKSAIYVFRYKILRIKPPTNRIGKYKEITMQK
                     SNNAIEEAIRKGEPFAAIRFGAVEISALNNYEKIRLGFRRKYKKSVVYSIKNNAGVFP
                     PTQEIIDFYAKYLETNLKDTDYLGVSGVHMENYFFKHLTPDATPVQNWALDPLLGKWS
                     HLLAGKRVLVISPFAEQIEEQYEKRHLLFQAEPEILPEFTLVTIPAILTSGKAASKEF
                     DNWFEVLDYLKLEILKKDFDIALVGAGAYGTPLCLYIKSLGKMAIQSGGATQLLFGLM
                     GKRWEKREYVARHVNPHWSRPTVKPYGHKNIEGGCYW"
     gene            complement(2502..>2762)
                     /locus_tag="PLQ55_03605"
     CDS             complement(2502..>2762)
                     /locus_tag="PLQ55_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HPK29049.1"
                     /translation="ISAMGAGNKIDPTKVIITKIEKTEGCPLAKKVRYRLRQEKLKGL
                     MVVTSKELPLTNAGNKPGSSAFVPPAFGLAMASYILRNVISK"
BASE COUNT          913 a          537 c          436 g          876 t
ORIGIN      
        1 aactgtttct tcgtaattac ttgttaaata acgttctggg atatttgtat aataattttc
       61 actaaagtct tgagtgtgaa atgcaccttt ttcaaaggtt tccgtaactg ctttacaacc
      121 tgtaagtgtt aagacactaa taaggagtgc tatccattta tttttccgca tgagttactt
      181 cctcctcttc tattttttta tcttctttta agacaaaaac ataccccgca ataaagactg
      241 tcactaaaaa tagtggtgaa ttaatcagcg ggaaattatt aatgtcacta aattcttgat
      301 aagtaaagat tttaaaagga taacggagca taaagcggaa gaatgctgtg agtaacacgc
      361 taatgagtac aacctttaat actctctcat ccttatcttt attgaaatac ttcactaaac
      421 gaatcacaaa catcacaaag aatgcaagta gagcaacaaa cccaattaaa ccgtcaatcc
      481 acattgcatc aaataagaag ttacctgaag ggaatacttt ataaccttca actgtcatcg
      541 caaagtgtcc atataaatta cggaatccaa atgcttcttt gaaaataata tagaagcgtt
      601 gcatgtagcc gttaaagaat aatttacgcg taacgcgatt agtttgccac actgcttgta
      661 agttaaagat attataagca gttagtaaga aaataatagc gccaagtccc actagggcaa
      721 taacgacacc aagcgtcact ttgtttttaa gtttctttaa gaggtcaaac ttaagtaaca
      781 aagcaaaaat aagcgctggt aataaataaa taagagccgc aaacgaggcg gtgaatatta
      841 aggtaaggac accaacagca ctaataacta cgagactaat gattgtaaat ttatctttct
      901 tttcatttac aaacaataag ccaaagcctg cgctagtaat aataaccgcc atcgatgcca
      961 gtgcttttaa gtcaccaacc attattctaa agccatacaa taatgacgct tgactactaa
     1021 cttcatattc atgtcctgcg taataagtta ctaaactttt gtacactaac gggtgaaaaa
     1081 atccgtaatt aataagtgta gcaaaaagcg aaattaaaac tggtgctgct agtccggcaa
     1141 gaataacaat aaatatatgt tttgtggcaa atacctcact tttgccgata aaatagccca
     1201 ataataaaag tccaacaatt cctaacaaat ttattaatcg cgtaataaat ggtagttgca
     1261 taaatacttc atttgaatac atttcaggat aagaaccgta tgctggtgcg actagcgtca
     1321 ctaacgcata aaaaagaatc ggcattaata gcacatataa accttggcct aaatcaactt
     1381 tcatttcttc aaaaaagaaa ggcgcaagaa tcgctgttaa caaaaacgct aatgctttaa
     1441 ataccggacc aatattacca aaatcaagcg caataaaaag cactacttct aatgctagaa
     1501 ataatgaaat aagcgccaat ttactttctc taattttatc tttcattacc agtaacaccc
     1561 tccttcaatg tttttatgac catatggttt aacagttggc cgtgaccaat gtggattaac
     1621 gtgacgcgca acatattcgc gcttttccca tcttttaccc ataagtccaa aaagtaattg
     1681 tgttgcaccg ccactttgaa ttgccatttt gcctaagctt ttaatatata gacaaagcgg
     1741 agtgccgtaa gcccccgccc cgacaagagc aatatcaaaa tccttcttta aaatctctag
     1801 ttttaaataa tcaagtactt caaaccaatt atcaaattct ttacttgctg ctttaccaga
     1861 agttaaaatc gctggaatcg tcactagtgt aaattccggt aatatttctg gttctgcttg
     1921 aaaaagcagg tgtctttttt catattgttc ttcgatttgt tcggcaaatg gtgatataac
     1981 aagtacgcgt ttgcctgcta aaagatgtga ccatttacca agaagcggat ctagtgccca
     2041 attttgcacg ggagtagcat ctggtgttaa gtgtttaaag aaataatttt ccatatggac
     2101 accgctaact cctaaataat ccgtatcttt taagttagtc tcaagatatt tagcgtaaaa
     2161 atcaataatt tcttgcgtag gcggaaatac cccagcgtta tttttaatgc tgtatacaac
     2221 ggacttttta tatttccgtc tgaaaccgag acggattttc tcataattat taagtgcact
     2281 tatttctaca gcgccaaatc gaatcgccgc gaaaggttcg cctttccgga ttgcttcttc
     2341 aatcgcgttg ttactttttt gcatcgtgat ttctttatat ttaccgatgc gatttgttgg
     2401 tggcttaatt cttaaaattt tatagcgaaa aacatatatt gcgcttttta agaattccca
     2461 tatatatgag cgacgtgttc ttttcataca tttatcatac actatttaga tattacattt
     2521 ctcaaaatgt aacttgccat cgctaatcca aaggctggtg ggacaaaggc actacttcct
     2581 ggtttattac cagcattagt taatggtaat tcttttgaag taacaaccat taatcctttc
     2641 agtttttctt gacgtaagcg ataacgcact tttttcgcta gtggacaccc ttctgttttt
     2701 tctattttag taataattac tttagttgga tcaatcttgt tgcccgcacc cattgcacta
     2761 at
//
LOCUS       DAOPCD010000122         2720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_29430_length_2720_cov_3.230019, whole genome shotgun sequence.
ACCESSION   DAOPCD010000122 DAOPCD010000000
VERSION     DAOPCD010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29430_length_2720_cov_3.230019"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLQ55_03610"
     CDS             complement(<1..237)
                     /locus_tag="PLQ55_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29050.1"
                     /translation="MKHITIVYDEKLRSFGIKVIKVRPGAFKTDMQINVTHKFEKVFA
                     YTKDFKSPLTKMQKMMLTELERAKDPRKITKTFLK"
     gene            complement(304..687)
                     /locus_tag="PLQ55_03615"
     CDS             complement(304..687)
                     /locus_tag="PLQ55_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK29051.1"
                     /translation="MKYALITGASGGLAHNIITAIKDDFFLILTDINPAISDKYNDLP
                     NKLIVVSDLTDKDSINNLFDKILKITDKLDLVIHFAGILEIGPIMEVKVEALETLMQV
                     NLFAVYFINQKAFPLLKKLKVALFI"
     gene            807..1484
                     /locus_tag="PLQ55_03620"
     CDS             807..1484
                     /locus_tag="PLQ55_03620"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HPK29052.1"
                     /translation="MKVFVYIFFGLFIAVTIAELIMAFNEIEKARMILKPFSMFFLAI
                     AALIAVPNHPLIYLGAFLGMVGDIFLLWPKNKKMFLAGATSFLVGHTLYISEIIHIMF
                     KGQALPWWFYAVVSFGVLLFVLAFYPMSKKISGDRYLTLVGNTYIAVLILVSVVSIIA
                     CVKGFTNFMVLGIIGGISFLASDLILVQATFVKDFKRRDYYIMLFYLLGQALIVSGLV
                     LTYVFAA"
     gene            1519..1737
                     /locus_tag="PLQ55_03625"
     CDS             1519..1737
                     /locus_tag="PLQ55_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29053.1"
                     /translation="MKKHNLLFITLTVVTLLSGCKKYRSRGSVLSGELLRNNITKVLK
                     KAEPLNVKKIYEQLYLIMMKFITIMTLH"
     gene            1797..2129
                     /locus_tag="PLQ55_03630"
     CDS             1797..2129
                     /locus_tag="PLQ55_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29054.1"
                     /translation="MRKQIIHNIFKKALVRDIYVTNNEVSGAFIRVYYDELYIFDGFG
                     HLLYQGTQEIHSISSGINNEGKHYVKLYINPVFTTPITYDEVYFYVYDALGNISKVAT
                     DPAPNTQK"
     gene            2148..>2720
                     /locus_tag="PLQ55_03635"
     CDS             2148..>2720
                     /locus_tag="PLQ55_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29055.1"
                     /translation="MSEYGLPLYSLVTNEESQEIKDATGKTIVTLKKEVGVYRSQIFA
                     GHYFYQKITKGSKSAINYDLKDIYGNTYKVSTHSVNLKTGRIKTLKFPYFLAAISPLY
                     DKNNIYNYAVAEIATFDAFNSKTANYVSEKFIINYKGHLLDQVSDMPLENFVEIKGGF
                     YNKATNKLYDEDVKLIKEFKSEFNILLYEDI"
BASE COUNT          867 a          388 c          503 g          962 t
ORIGIN      
        1 ttttaaaaat gttttagtga ttttacgggg gtctttagct ctttctaact cggtaagcat
       61 catcttttgc atttttgtaa gcgggctttt aaagtcttta gtgtaggcaa atactttttc
      121 aaatttatgc gtaacattaa tttgcatatc tgttttaaaa gcaccgggtc taactttaat
      181 aaccttgatg ccaaaacttc gtaatttctc gtcgtaaact atcgttatat gcttcaatcc
      241 tgcgttttgc tatcgtgtag aaggagtgga agggtagtgc taaaagtgtg ccgtactcac
      301 tacttaaatg aataatgcga cctttagctt ttttaagagc gggaaggctt tttggttaat
      361 gaagtagacc gcaaaaagat taacttgcat caaggtttca agtgcttcaa cctttacttc
      421 cattattgga ccaatctcta aaataccggc gaagtgaatg actaaatcaa gcttgtctgt
      481 aattttaagt atcttatcaa aaagattatt tattgagtct ttatctgtta agtcgctaac
      541 cacaataagt ttatttggta aatcattata cttatcactt attgctggat taatatcagt
      601 gagtattaaa aagaagtcat ctttgatggc agtgataatg ttgtgcgcta agccaccact
      661 agcgccagta ataagcgcat atttcataaa tttattttaa cactctaaat gtaaatgagt
      721 gtcaatattt ggaaaatagc ggtaaattca ctacttttta ataatattaa aagatgataa
      781 tataaaaggg taggagaaat atagttatga aagtatttgt gtatatcttt tttggtttat
      841 tcattgcggt gacaattgct gaactaatta tggcttttaa tgagattgaa aaagcgcgga
      901 tgattcttaa acctttttca atgttctttt tagcaattgc ggcacttatt gctgtaccta
      961 atcacccgct tatttatctt ggagcatttt tagggatggt tggtgatatc ttcttactgt
     1021 ggcctaaaaa taagaagatg tttcttgcag gggcaacaag ctttttagtt ggacatacat
     1081 tatatattag tgaaatcatt cacattatgt ttaaaggtca agctttaccg tggtggtttt
     1141 acgctgtggt gagttttggc gtgttgctct ttgtgttagc gttttatcca atgtccaaaa
     1201 agattagtgg tgaccggtat ttaacacttg ttggtaatac atatattgct gttttaattt
     1261 tagtttctgt tgtgagcatt attgcttgtg ttaaggggtt tactaacttt atggtactag
     1321 gtatcatcgg tgggattagc ttcttagcga gtgatttaat cttagtacag gcaacttttg
     1381 tcaaagattt taaacgccgc gattattaca ttatgctttt ctatttactt gggcaagcat
     1441 taattgtttc tggattagtg ctcacttatg tctttgcggc ctaatttcta atatcgatat
     1501 tggtaaagga ggaacttagt gaaaaaacat aatttacttt ttattacttt aaccgttgtg
     1561 actttacttt caggatgtaa gaagtatagg tcgcgtggta gtgtgttaag cggggagtta
     1621 ctgcgtaaca acattacaaa agtgctaaaa aaagcagaac cgttaaatgt taaaaaaata
     1681 tatgaacaat tgtatttaat catgatgaaa tttatcacca ttatgacact gcactaatta
     1741 gagatataat tgcgacgaaa aatggtgatg attgtattgg tttttactca ttagcgatgc
     1801 gtaaacaaat cattcataat atatttaaaa aagccttagt gcgtgatatt tatgttacta
     1861 ataatgaagt atcaggcgcc tttattcgtg tgtattacga cgaactttat atttttgatg
     1921 gctttggtca tttactctat caaggcacac aagagattca tagtataagt tcagggataa
     1981 ataatgaagg aaaacattat gtaaaactat atattaatcc cgtattcact actccaatta
     2041 cttatgacga agtttatttt tatgtgtatg acgcccttgg taatatatca aaagttgcga
     2101 ctgatccagc gccaaataca caaaaataaa gtagtaacgc ggaagatttg tcagagtacg
     2161 gattacctct ttactcgcta gttacaaatg aagagagtca agaaattaag gacgctactg
     2221 gtaaaacgat agtgacgcta aaaaaagaag taggcgttta tcgtagccaa atctttgcgg
     2281 gtcattattt ctatcaaaag atcacaaaag gaagcaaaag tgcgattaat tacgatttaa
     2341 aagatattta tggcaacact tataaagtgt caacacatag tgttaattta aaaacaggtc
     2401 gcattaaaac gcttaagttc ccctactttt tagccgcgat aagtccactc tatgataaaa
     2461 ataacattta taattatgct gttgctgaga ttgctacttt tgatgctttt aatagcaaaa
     2521 cagcaaatta tgtttccgag aaatttatta ttaattataa aggtcacttg cttgatcaag
     2581 taagtgatat gccgcttgaa aattttgtgg aaattaaggg cggattttat aataaagcta
     2641 cgaataaact ttatgatgaa gacgttaaat taattaaaga atttaaatct gaattcaata
     2701 ttttacttta tgaggatatt
//
LOCUS       DAOPCD010000123         2712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_29556_length_2712_cov_2.907414, whole genome shotgun sequence.
ACCESSION   DAOPCD010000123 DAOPCD010000000
VERSION     DAOPCD010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29556_length_2712_cov_2.907414"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..120)
                     /locus_tag="PLQ55_03640"
     tRNA            complement(44..120)
                     /locus_tag="PLQ55_03640"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(84..86),aa:Thr,seq:cgt)
     gene            203..946
                     /locus_tag="PLQ55_03645"
     CDS             203..946
                     /locus_tag="PLQ55_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29056.1"
                     /translation="METKKKRTINIILKISIIVAAIIGIISQQLHGLLIYTGHVPFMY
                     FTTQSNILVAVCMGILLYYDLTEKEVPRWVLIFQHITVTAVTLTFLVFAIFLGPFIPD
                     LSVGYFYSVQNLTLHNIVPILAISQYLYDDNRDIPKYAKYLGLSSGFIYVIFVYIVHL
                     CGATFGATEFPYFFLDYKEFGWLTINEGKIGIIYWWIVIILLLLGISALLYYLKKKSA
                     ERKNIPVAIFNSLIILSIIFITINIIIKI"
     gene            969..>2712
                     /locus_tag="PLQ55_03650"
     CDS             969..>2712
                     /locus_tag="PLQ55_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29057.1"
                     /translation="MKLRKLKAVPLALVTLFTLTSCKLIDNIIGSILVNTVMDLPVLI
                     NRLDLMPSAENTNPTFVPFENPPSSEANRAELNYLKNVLVVPRDLETVVFNKTIKLHF
                     DFEFSGEGATNAFHVIDVTESDLPENFRFDTSAFPVPITYKLALIVPVGESTILEGVT
                     NINQIQTVLEDTPRDELVELIDQEPLNATMKVIARSAGKSKSKNFYFNLTKPLFGAMV
                     LEEVFKSGYLINLYRIHGFNQENNEVESTIENVADLPSESENPLNLSYFLDSIAIPKI
                     VTLKDLGDVTFEVTTNRPDLLFIGEMSKPIEDLDPGNELITGDVTVHTLTPVGTYSSE
                     IPASVDTVDKLKDHLKSVDVKELYPYATQPTQDFSITITATCFGEERTETYYFRLQEP
                     PLDQEIVDYAIKSEYMINKVFYKDTTSIIRTTRENMPSTPEKRMKVSYLTETLAIPRH
                     VVLSDFGNKELDFSINVLGNNADKFYMSEQTDTLDGNPVTVKTLTPVGGLAYTTPQND
                     LPAFAEEIKGLSREDLYDCVTQTSIDVDIELSATLNGKTATKTYYFTLVAADIDVEII
                     DYLIDDSSLLNVVTY"
BASE COUNT          869 a          491 c          466 g          886 t
ORIGIN      
        1 attatacctc tctccaagat ttggaagcta attcaatttt gactggtgcc gacgacagga
       61 attgaacccg caacctactg attacgattc agttgctctg ccagattgag ctacgtcggc
      121 ctagtcttaa tcatatcgac tttatcacaa tattgcaata aaaacatcaa gaaaatatct
      181 tgatttgtag tataatcttt ttatggaaac taagaaaaaa cgcactatta acattatctt
      241 aaagattagt attatcgttg ccgcaattat cggaataata tcacagcaac tgcatggttt
      301 attaatttac acgggtcacg taccatttat gtactttaca acccaaagta atattcttgt
      361 cgccgtttgt atgggaattt tactatatta cgacttaact gaaaaggaag ttccgcgctg
      421 ggttttaatc tttcagcata ttacagttac cgctgttaca ttaacctttt tagtgtttgc
      481 tattttcctt ggtccattta ttcctgatct tagcgttggt tatttttatt ccgtgcaaaa
      541 tttaacatta cataatattg tcccgattct tgctatcagc caatatttat atgatgataa
      601 tcgtgatatt ccaaaatatg ctaaatatct aggcctttct agcggattta tttatgtaat
      661 ttttgtgtat attgttcacc tttgtggcgc tactttcggc gcaacagagt ttccttactt
      721 cttccttgac tataaagagt ttggatggtt aacaataaat gaaggtaaaa tcggcattat
      781 ttattggtgg atagtaataa ttttattact acttgggatc agtgcactct tatattactt
      841 aaagaaaaaa agtgctgaac gcaaaaatat tccggttgca atttttaata gtctaattat
      901 tttatctatc atttttataa caattaatat tataattaaa atataaagaa ggtttagggg
      961 gttctattat gaaacttcgc aaattaaaag cagtcccatt ggcgcttgtc accttattta
     1021 ctcttactag ctgtaagcta attgataata tcatcggatc aattcttgtc aataccgtga
     1081 tggatttacc tgtgcttatt aatcgccttg atttaatgcc aagtgcagaa aacactaatc
     1141 caacatttgt gccatttgaa aatccaccaa gtagtgaggc taatcgcgct gaacttaact
     1201 atttaaaaaa tgtattagtc gtcccgcgcg atcttgaaac ggtcgtcttt aataaaacga
     1261 ttaaactcca ttttgatttt gagtttagtg gtgaaggagc aacaaatgcc ttccatgtca
     1321 ttgatgtaac ggaaagtgac ttaccggaaa acttccgctt tgatacaagc gctttcccag
     1381 tacccatcac ctacaaatta gcgctgattg ttcctgttgg cgaaagtacg attcttgaag
     1441 gtgttactaa tatcaatcaa attcaaacgg ttttagaaga tacaccacgt gatgagttag
     1501 tagaattgat tgaccaagaa ccacttaacg ccacgatgaa agtcattgcg cgctccgctg
     1561 gtaaaagtaa aagcaaaaat ttctatttta atttaactaa accattattt ggagcaatgg
     1621 tacttgaaga agtatttaaa tctggttact taattaatct ttatcgtatt cacggtttta
     1681 accaagaaaa taatgaagtt gaaagtacaa tcgaaaatgt cgcagactta ccaagtgaaa
     1741 gcgaaaatcc acttaatctt tcttatttcc ttgattcaat tgccattcca aaaattgtta
     1801 cacttaaaga cttgggcgat gtcacttttg aagtcacgac gaatcgtcct gatttattat
     1861 ttattggtga aatgagtaag ccaattgaag atttagatcc cggtaatgaa ttaattacag
     1921 gggatgttac tgtgcacact ttaacacctg ttgggactta cagtagtgaa attcccgcaa
     1981 gtgtcgatac tgtcgataaa ttaaaagatc acttaaagag tgtggatgta aaagaattat
     2041 atccttacgc tacgcaacca actcaagatt ttagcatcac cattaccgcg acttgttttg
     2101 gtgaagaacg gacagaaact tattacttta ggctacaaga accaccgctt gaccaagaaa
     2161 ttgttgatta tgcaattaaa agcgaataca tgattaataa agtattttac aaagacacca
     2221 caagtattat tcgtacgaca cgtgaaaata tgccttccac tccagaaaaa cggatgaagg
     2281 tatcgtactt aaccgaaact ctagccatac cgcgccatgt agttttaagt gactttggta
     2341 acaaagagct agacttctca attaatgtat taggtaataa cgctgataaa ttttatatgt
     2401 ccgaacagac tgatacgctt gacggtaacc ctgtcactgt taagacatta acaccagtcg
     2461 gcggtttagc ttacacgaca ccgcaaaatg atttaccagc atttgccgaa gaaattaaag
     2521 ggctctcgcg tgaagatctt tatgattgcg ttacccaaac tagtattgat gttgatattg
     2581 aactaagtgc tacacttaac ggtaaaaccg cgacaaaaac atattatttc acacttgtgg
     2641 ccgcagatat tgatgtggaa attatcgatt acttaattga cgatagttct ttactcaatg
     2701 tcgttactta tg
//
LOCUS       DAOPCD010000124         2683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_29980_length_2683_cov_2.670091, whole genome shotgun sequence.
ACCESSION   DAOPCD010000124 DAOPCD010000000
VERSION     DAOPCD010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29980_length_2683_cov_2.670091"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PLQ55_03655"
     CDS             complement(<1..394)
                     /locus_tag="PLQ55_03655"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29058.1"
                     /translation="MQLIEVFTQYSKLNRPFTYYYDGDDLEVGVRVLIDFNNREIVGY
                     VAAVKSTKETIETASKRLGFELAEITKILDEKPLLTNELMELSENVTSYYLTNRISVL
                     NAMLPPSLRPSKGSLSAPKIAMQKKVSFN"
     gene            complement(397..969)
                     /gene="gmk"
                     /locus_tag="PLQ55_03660"
     CDS             complement(397..969)
                     /gene="gmk"
                     /locus_tag="PLQ55_03660"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPK29059.1"
                     /translation="MRQGFIVLISGPSGVGKGTLIHSLLADQTLNLSFSVSMTTRKPR
                     PNEKDGIHYFFVSHETFLKNIEENNFLEHAQFVGNRYGTPRDYVEKLLNEGKNVLIEI
                     EVDGARQLLKNVDSEKVLSLYIMPPSVQELVRRLRLRGSEDEATIQRRAERFKEEIKY
                     KDQYQHIIINDELETCINDVKALIREKMNI"
     gene            1058..2413
                     /locus_tag="PLQ55_03665"
     CDS             1058..2413
                     /locus_tag="PLQ55_03665"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HPK29060.1"
                     /translation="MQRLFPNDLQQLINANKHFEGLFLVRIRQLSLVDFVFEFSHGNN
                     LFVSLNSPTPFIVFNADLSDFPEGGELHSFAINLRQFLGGNLVAMNQPNDDMILSLVF
                     EQRNNVLELEEVTLFIELIPRHPQAVLVNNKGRILAAYRYHQERAKDGRFIRGGSKYS
                     LPEKGAFSPQISNAKTDYLQAYLKENRERIKKQNFRDVYVYLETNIKRSKRLIRNYEQ
                     DLEKLKELPDLYHKANMLLTYKPAISYYFVQIEGETIEVDPRFDALFNAEILFKKAKK
                     IKKSENILMSKIAETKEKIKYLESIENHLKSLESAGEIFQIYEELGLLKRRDKRQIIS
                     KLNPYVITYKDIDILFGKNNKQNDFLTFKIAKKNDTFMHIQNRPGAHIIIRQENPAKD
                     VLEFAGMLCLFLAKQIDGEVMYSPIYNIKKGAFPGQVLTRKYNTFFLTKLCGINTASK
                     L"
BASE COUNT          927 a          419 c          398 g          939 t
ORIGIN      
        1 gattaaaact tacttttttt tgcattgcaa tctttggcgc acttaaacta cctttactag
       61 gccgtagtga gggtggtaac atcgcattta aaacactaat ccgatttgtt aagtaataac
      121 tagtaacatt ttcactcaat tccattaatt cgttagttaa gagtggtttt tcatcgagaa
      181 tctttgttat ttcagcaagc tcaaagccta ggcgtttact tgccgtttca attgtctctt
      241 tggtgctttt gacggccgct acataaccaa caatttcacg attattaaaa tcgatgagaa
      301 ctcttactcc aacttctaaa tcatcgccat cataataata tgtaaaagga cgatttaatt
      361 ttgaatattg tgtaaaaact tcaatgagtt gcatatttaa atattcatct tctcccgaat
      421 taatgcctta acatcattaa tacacgtttc taactcatca tttatgataa tgtgctgata
      481 ttggtcttta tatttaattt cttctttaaa tctttcagcg cgacgctgta ttgttgcttc
      541 gtcttccgag ccacgtaagc gtaagcgtct tactaattct tgaactgaag ggggcattat
      601 ataaagggaa agaacttttt cactatcaac attttttaat aactgacggg caccatcaac
      661 ttcaatttca attaaaacat ttttgccttc attcaataat ttttcaacat agtcgcgagg
      721 cgttccatag cgatttccaa caaactgagc atgctctaaa aaattgtttt cctcgatgtt
      781 tttcaaaaat gtttcatgac taacaaaaaa ataatgaatc ccatcttttt cattaggacg
      841 tggttttctt gtagtcattg aaacgctaaa acttaagttc aatgtttgat cggcaagtaa
      901 actatgaata agtgttcctt tgcctacacc tgatggacca cttattaaaa caataaagcc
      961 ttgtctcata tattttcccc ttgattttcc ttaataaatt gtatcttatt tattaataaa
     1021 taacaaactt gtcacattct tgttgtagaa taagagcatg caaagacttt ttccaaatga
     1081 tttacaacaa ttaattaacg ctaataaaca ttttgaaggt ttatttctag tgcgaattcg
     1141 tcaattaagt ttggttgatt ttgtatttga atttagtcat ggtaataact tatttgtctc
     1201 attaaatagt ccgacgcctt ttattgtgtt taacgctgat ttaagtgatt tccccgaggg
     1261 cggagaatta catagttttg ccataaattt aagacagttt ttaggtggta acttagtggc
     1321 tatgaatcaa cctaatgatg atatgatttt atcactagtt ttcgagcaaa gaaacaatgt
     1381 tttggaactt gaagaagtca cactttttat tgaattaatt ccgcgtcatc cccaagctgt
     1441 attagtgaat aataaaggac gtattttagc agcgtatcgc tatcatcaag aacgcgcaaa
     1501 agatggtcga tttattcgtg gtggcagtaa atattcacta cctgaaaaag gtgctttttc
     1561 gccgcaaatt tctaacgcta aaacagatta tttacaggca tatcttaaag agaatcgtga
     1621 acgaattaaa aagcaaaact ttcgtgatgt ttatgtatat ttagaaacaa atatcaaacg
     1681 ctcaaaacga ctaataagga attatgagca agacttagaa aagttaaaag aacttccgga
     1741 tttataccac aaagcaaata tgttattaac atataaacca gcaatttcgt actattttgt
     1801 gcaaattgaa ggtgaaacta tagaagttga tccgcgtttt gatgcacttt ttaacgctga
     1861 gattcttttc aaaaaagcta aaaaaattaa aaaatccgaa aatatattga tgtcaaaaat
     1921 tgctgaaact aaagagaaga taaagtatct ggaaagtatt gaaaatcatt taaaaagtct
     1981 agagagtgct ggagaaatat tccaaattta tgaagaactt ggactattaa aaagacgtga
     2041 taaacggcaa ataatttcta aattaaatcc gtatgtaata acttataaag atattgatat
     2101 tttatttgga aaaaataata aacaaaacga ctttttaacc tttaaaattg ctaagaaaaa
     2161 tgatactttt atgcatattc aaaatcggcc tggagcgcat ataattatta ggcaagaaaa
     2221 tccagcaaaa gatgtgttag agtttgctgg aatgttatgc ttatttttag caaaacaaat
     2281 tgatggtgaa gttatgtatt caccgattta taatattaaa aagggggcgt tccctggaca
     2341 agtactaacc cgtaaatata atacattctt tttgactaaa ttatgtggaa taaatactgc
     2401 atctaaatta taatacgaaa atataatatc atatttttga ttattatcat ctaaatattc
     2461 atataatttt tgattataat atatagattc aacattccaa cgacatctat cttctccagg
     2521 tttaatatca tatatgaatt ttattttttc gtcttgattt aactcaacaa aattaatttc
     2581 atctttatat gaactataaa atttttgata ttgttttgaa taatttaaag caacactttt
     2641 cggcgctatt gtgaaaaaat atacctcatg tcccaatttt aaa
//
LOCUS       DAOPCD010000125         2656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_30370_length_2656_cov_3.280661, whole genome shotgun sequence.
ACCESSION   DAOPCD010000125 DAOPCD010000000
VERSION     DAOPCD010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30370_length_2656_cov_3.280661"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1085)
                     /gene="rlmN"
                     /locus_tag="PLQ55_03670"
     CDS             complement(21..1085)
                     /gene="rlmN"
                     /locus_tag="PLQ55_03670"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPK29061.1"
                     /translation="MSKKLNFYDYSLPKLQDFLLNEGFKKYSATQMFEWVYLKDELDF
                     NNMSNLALVTREFMINNFTFALPTVHELQESKDGTVKLLLKLGDGSLVETVLMRYRYG
                     NSVCVSTQVGCNMGCRFCASGLLKKKRDLTPGEMLGQVLMINEYLKGRGELKLTTHVV
                     LMGTGEPFDNFENILDFIDLLNNPKSLAIGARHITVSTVGLVPKIKEFADLKLQVKLA
                     ISLHAATDEKRNQIMPINNAYPLKEVLSAVKYYESKTNKRVTYEYIMLNDFNDTLEDA
                     RALVNLVKGTNGFVNLIAYNPIAEFDYEPSTRDHVYKFANYLKSMGVQTTVRKEFGSD
                     IDAACGQLRAKVEKKRGNNS"
     gene            complement(1069..2286)
                     /locus_tag="PLQ55_03675"
     CDS             complement(1069..2286)
                     /locus_tag="PLQ55_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29062.1"
                     /translation="MIKHILDTTEKIIDGSLPYQQFSKDLKQDNSYSQSEKHQFRTLV
                     NALLNRYFRYLYSLTEGEETTLTQFEVLALSLFSFNREKKVVPLSYEEFLAALPTDIQ
                     VKFEQEYPDESFPIRIPKHFHSENPALSLAARFSVPLALTEQLIADIGRKNLLKFLTN
                     RYRETYGLINQNVISDEDFFANMPLFIRGLKPGEYEYKGKEYIKKTPAYNNNEIVIAS
                     QTLLDVVDIIESKKPKNLLFVQHEDTSLMLVLALKYPEIQIHFYKDDNRSKYIVRHLV
                     RKFSLENVTYVDKITQTYDFVLTTLQSSNINGDIRHRDFYFRLPDNLQFFAAKGREEL
                     EELKDYVNEDGHLMFITTTVLRFETHLQAVGFVRDYPEFELILEKQHFHFTPNNETLY
                     YALLKKGAHVEEA"
     gene            complement(2347..>2656)
                     /locus_tag="PLQ55_03680"
     CDS             complement(2347..>2656)
                     /locus_tag="PLQ55_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29063.1"
                     /translation="ALIAEGVDQVSWFTYLEVTIPANLKHKQDALSKTLLEIFNNYGV
                     HLMIKYDYVTKDNIVKILNPDFKVFNETMVEVLPTGNYYDEDDVDWDDDWDEDEDKDD
                     "
BASE COUNT          850 a          477 c          409 g          920 t
ORIGIN      
        1 ttttagcagc gaatgttcct ttaactgttg ttgccacgtt tcttctccac ttttgcccgt
       61 aattgtccgc aggccgcatc tatatcacta ccaaactctt tacgtacagt tgtttgcact
      121 cccatgcttt ttagataatt agcaaattta taaacatgat cacgagttga tggttcataa
      181 tcaaattccg caattgggtt atacgcgatt aaattaacaa aaccatttgt gcctttaact
      241 aggttaacta aggctcttgc gtcttccaaa gtatcattaa aatcattaag cataatatat
      301 tcataagtca cgcgcttatt agttttactt tcatagtatt tcacggcaga aagtacttct
      361 tttaaaggat aagcattgtt aattggcata atttgattcc gcttttcatc agtagccgca
      421 tgtaaagaaa tggctaactt tacttgtaat tttaaatcgg cgaattcttt aattttaggg
      481 actaaaccca ccgttgaaac tgtaatatga cgagcaccaa tcgcaagtga ttttgggtta
      541 tttaataagt caataaaatc taaaatattt tcaaagttat caaacggctc cccagtcccc
      601 ataagtacga catgcgttgt aagttttaat tcaccacgac cttttagata ttcattaatc
      661 attaatactt gaccaagcat ttcaccaggt gttaagtcgc gctttttctt taaaagccct
      721 gacgcacaaa agcgacaccc catattgcat ccgacttgcg ttgaaacaca gacgctattg
      781 ccataacgat agcgcatcaa aactgtttcg acgagtgatc catcaccaag ctttaaaaga
      841 agtttaacag taccgtcttt actttcttgt agttcatgaa ctgttggtaa tgcaaaagta
      901 aaattattaa tcataaattc gcgtgtaact aacgctaaat tactcatatt attaaaatct
      961 aattcgtctt ttaaatagac ccactcaaac atttgagttg cactatattt tttaaacccc
     1021 tcatttaata aaaagtcttg gagtttaggt aaagaatagt cataaaaatt aagcttcttc
     1081 gacatgggca cctttcttta aaagggcata atagagcgtt tcattattag gtgtaaagtg
     1141 gaaatgttgt ttttcaagta ttaattcaaa ctctggataa tcacgcacaa aacccactgc
     1201 ttgcaagtga gtttcaaacc gcaatacggt agtcgtgata aacattaaat gtccatcttc
     1261 atttacataa tcttttagct cttctaactc ttcccggccc tttgcagcaa agaattgtaa
     1321 gttgtctggt aaccggaagt aaaaatcacg atggcgaata tcaccattaa tatttgaact
     1381 ttgcagtgtt gtgagcacaa agtcataagt ttgggtaatt ttatctacat aagtaacatt
     1441 ttctaatgaa aacttccgta ctaaatggcg cacgatatat tttgagcgat tatcatcttt
     1501 ataaaaatga atttgaattt caggatattt aagcgcaagt acaagcatta aggatgtatc
     1561 ttcgtgttgc acaaataaca aatttttagg ttttttgctt tcgataatat cgacaacatc
     1621 taataatgtt tgactagcaa ttacaatttc attgttattg tacgctggtg ttttcttgat
     1681 atactctttc cctttatatt catattcacc aggttttaag ccacgaataa aaagtggcat
     1741 attagcaaag aaatcttcat cactaattac attttgatta ataagaccat atgtttcacg
     1801 atatctattt gtaaggaatt taaggaggtt tttccgtcct atatctgcaa ttaattgttc
     1861 ggtaagtgct aatggtacac taaagcgagc ggctaaagaa agcgctgggt tttcactatg
     1921 aaaatgtttt ggaattcgaa ttggaaaact ttcatcagga tattcttgtt caaatttaac
     1981 ttgtatatca gtaggaagcg cagcaagaaa ctcttcataa gataaaggca caactttttt
     2041 ctcacgatta aaactaaaga gacttaatgc taaaacttca aattgagtta gagttgtttc
     2101 ttcgccttca gttaaagaat acaaataacg gaagtatctg tttaaaagag cattaactaa
     2161 agtacgaaac tgatgcttct cactttgtga gtaactatta tcttgcttta agtccttact
     2221 aaattgttga taagggagac tcccatcaat gattttttct gttgtgtcta aaatgtgttt
     2281 tatcatatgt acctacctat attagtatat cactcttttt aaaggtgttt ctaattaatt
     2341 agaaaattag tcgtctttgt cttcgtcttc gtcccaatca tcatcccagt cgacatcatc
     2401 ttcatcgtag taattgccag taggcaaaac ttcaaccatt gtttcattaa aaactttaaa
     2461 atcagggttt aaaattttaa caatattatc ttttgttaca tagtcatatt taatcataag
     2521 gtggacacca taattattga atatttctaa aagcgttttg cttaatgcat cttgtttgtg
     2581 ctttaaattt gcaggaatag taacttctaa atatgtaaac caacttactt gatcaacacc
     2641 ttccgcgatt aaagca
//
LOCUS       DAOPCD010000126         2641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_30579_length_2641_cov_3.635731, whole genome shotgun sequence.
ACCESSION   DAOPCD010000126 DAOPCD010000000
VERSION     DAOPCD010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30579_length_2641_cov_3.635731"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PLQ55_03685"
     CDS             <1..306
                     /locus_tag="PLQ55_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29064.1"
                     /translation="LMTNELNNFLHLLKRSGHYRFGADYLRESNARKIALVLTSDDLS
                     EYTKTKVNNFTLAHNIAQFDVPANIMSMLYPGKTIKVLVVTNHQSAQKIANLMKEGN"
     gene            308..2134
                     /gene="infB"
                     /locus_tag="PLQ55_03690"
     CDS             308..2134
                     /gene="infB"
                     /locus_tag="PLQ55_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006506691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPK29065.1"
                     /translation="MSNEKKRISNVAHHSKKQSGPCVEGRKLIYQGPIKVSDLARLLK
                     LSEAQIIKNLFMKGQMVTINNVLSPETVAELCLDFNFDYDFKEETEDDCDFGLSHIVD
                     DPKDLKPRPPVVTIMGHVDHGKTTLIDTIRSSSIVSGEAGSITQAIGAYQKEFHGKKI
                     TFIDTPGHEAFTAMRSRGTKVTDIVILVVAADDGVMPQTIEAIDHARAAEVPIIVAIN
                     KMDKPGVDPEKVKNELLAQNVIAEEYGGDVIFQPISAKKGTGIDELLDAILVTAEMLE
                     LEANPKRYAYGTVLEARLERGEGPKAMLLIQNGTLTNRDFVVAGAAYGKVRRMTNEHN
                     KALKSAGPATPVAIIGLDEVPEAGDNFIAFETEKEAKELATKRKQIRIEESRGSSEGM
                     KLSDLHKQIAEGETVVVNVIIKADNSGSAEAVRGSLEKINVEGVRLNIIRASAGQITE
                     SDVLLASASNAVIFGFNIRPNNQVRDLAERLKIEIHLHRVIYALLEQTEQALKGLLKP
                     EFEEKVTGQAEVRAIWHASKVGTIAGCYTLSGVIRRNQTVRLLRDGVIIYEGEVATLK
                     RVKDDIKETRAGYECGMTLLNYNDIKEGDIIEGYAMVEVKDA"
     gene            2127..2468
                     /gene="rbfA"
                     /locus_tag="PLQ55_03695"
     CDS             2127..2468
                     /gene="rbfA"
                     /locus_tag="PLQ55_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPK29066.1"
                     /translation="MPKSRNLSRNESLMLRHLSEIISYKVGDPSLGLATVSAVELTRD
                     YSYAKVYISFLGDGDVEAKMEILNNASGFIRHELSQKMNIRKTPELRFIYDDTFERVA
                     KLDELLKRTKK"
BASE COUNT          883 a          457 c          576 g          725 t
ORIGIN      
        1 ttaatgacta acgaacttaa taacttcctt catctcttaa agcgcagtgg ccattatcgc
       61 tttggcgctg attacttaag agaaagtaac gctcgtaaaa tcgcactagt gctaactagt
      121 gatgacttaa gcgagtacac aaagactaag gtgaacaact tcactttagc gcataacatc
      181 gcacaatttg atgtccctgc gaacatcatg tctatgcttt atcctggtaa aacaattaaa
      241 gtcttagttg tcactaacca tcaaagcgct caaaaaatcg caaatctaat gaaagaaggt
      301 aactgaaatg agtaatgaaa aaaagcgcat cagtaatgtt gcacaccaca gtaaaaaaca
      361 aagtggccct tgtgttgagg ggagaaaact tatttatcaa ggcccgatta aagtaagcga
      421 tttagcccgg cttttaaaac ttagtgaggc gcaaattatt aagaatttat ttatgaaagg
      481 gcaaatggtc acgattaata atgtgttaag ccctgaaaca gtggccgaac tatgtttaga
      541 ctttaacttt gattacgatt ttaaagaaga aacagaagat gattgcgact ttggattatc
      601 gcatattgtt gatgatccaa aagaccttaa accgcgtcca ccagtagtga cgattatggg
      661 tcatgttgat cacggtaaaa caacactgat tgatacaata aggagctcat caattgtcag
      721 tggcgaagca ggtagtatta cccaagccat cggtgcttat caaaaagaat ttcatggtaa
      781 aaagattaca tttatcgaca ctcctggaca cgaagccttt accgcaatgc gtagtcgcgg
      841 gactaaagta actgatatcg tcattttagt tgtggccgca gatgatgggg taatgccgca
      901 aactattgaa gcaattgacc acgcccgcgc tgccgaagta ccaattattg tcgcgattaa
      961 taaaatggat aagccaggtg ttgatcctga aaaagttaaa aatgaacttt tagcgcaaaa
     1021 cgtgattgct gaagaatacg gaggtgacgt tatttttcaa ccaatttctg cgaaaaaagg
     1081 aacgggtatt gatgaactac ttgacgcgat tcttgtcact gctgaaatgc ttgaattaga
     1141 ggctaatcca aagcgctatg cctacggcac agttttagaa gcgcgtcttg aacgcggtga
     1201 aggaccaaaa gctatgcttt taatacaaaa cggcacctta acaaatcgtg attttgtcgt
     1261 tgccggagca gcttatggta aagtacggcg gatgactaat gagcataata aggcattaaa
     1321 aagtgctgga ccagcgacac cagtcgccat tattggtctt gacgaagtac ctgaagcggg
     1381 tgacaatttc atcgcttttg aaacagaaaa agaagcaaaa gaactcgcta ctaaacgcaa
     1441 acaaattcgc attgaagaga gccgcggtag tagtgaaggg atgaaactta gtgatctcca
     1501 taagcaaatt gccgaaggtg aaactgttgt cgttaatgtg attattaagg ctgataatag
     1561 cggtagtgcg gaagcagtac gcgggagtct tgaaaagatt aatgtcgaag gtgtgcgcct
     1621 taatattatt cgtgcaagtg ctggccaaat taccgaaagt gatgtcttac ttgcaagtgc
     1681 gagtaatgca gttattttcg ggtttaatat tcgtccaaat aaccaagtgc gtgaccttgc
     1741 agaacgacta aaaattgaaa ttcatttaca tcgcgtaatt tatgctttac ttgaacaaac
     1801 tgaacaagca ctaaaaggtt tattaaaacc tgaatttgaa gaaaaagtta cgggccaagc
     1861 cgaagtacga gcaatttggc acgcttcaaa ggttggcact attgcgggat gttatacgct
     1921 gagcggtgtc attcgccgta atcaaacggt tcggttgctg cgtgatggcg tcattattta
     1981 cgaaggtgaa gtagcgacac tgaaacgcgt caaagatgac attaaagaaa cacgcgcggg
     2041 atatgaatgc gggatgactt tacttaacta taacgacatt aaagaaggcg atattattga
     2101 aggctatgcg atggtggagg ttaaagatgc ctaaaagtcg taatttatca cgtaatgaat
     2161 cattaatgct ccgtcatctt agtgaaatta ttagctataa agtaggtgac ccaagtttag
     2221 ggcttgctac tgttagtgcg gttgaactta cgcgtgacta tagttatgca aaagtatata
     2281 ttagtttttt aggggatggc gatgttgagg cgaagatgga aatcttaaat aacgcaagcg
     2341 gatttattcg ccatgaacta tcgcaaaaaa tgaatattag aaagacacca gaactacgct
     2401 ttatttatga cgatactttt gaaagagtag cgaaattaga tgaattatta aagcgtacta
     2461 aaaagtaaag ttaattatta ataagaaaaa taggcctgat tataaaggcc tattttattt
     2521 ttaattgaca agaagtgtct aattttaaat aactttcttt tcaaagtgat aatgtaagat
     2581 accggcgtcc atataacttt caccaacgcg tttataccct aagctttcgt aatattttaa
     2641 t
//
LOCUS       DAOPCD010000127         2615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_30965_length_2615_cov_3.012891, whole genome shotgun sequence.
ACCESSION   DAOPCD010000127 DAOPCD010000000
VERSION     DAOPCD010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30965_length_2615_cov_3.012891"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1760
                     /locus_tag="PLQ55_03700"
     CDS             <1..1760
                     /locus_tag="PLQ55_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HPK29067.1"
                     /translation="NKSQILRELGAIEQNRNVDGVVEVRDESDRNGIRIVIELKKEAN
                     TDVILEYIFSKTRLRAGYNANIVAIADNRPKTLNILDLIDAYISHQVDVITRRSEFDL
                     ATNEKRLHIIEGLIKAISVVNEIIAIIRKSTDKANAKTNIKAAYGFSEEQAEAIVMLQ
                     LYKLSNTDITVLMEEKAAIETTIDELRNILSNEKALNNVIIRDLKRIAQKFGDDRRTK
                     IREKGEVVQIDKRDLIADEQVMLALTRDGYIKRSSLKSYRSSGDFALPGVKSSDLLIA
                     ASEVNTKDFLVAFTDLGNYLYIPIYEIGEGRWKDEGKHINYIVQLNANEKIIKAFNVK
                     EFREDLYYAIATRNGMIKRTSLNEFVAVRYSRPLQAMRLLSGDRVADVTLTTGNSELL
                     VITESGAASFYNENEITVVSVKASGVKSINKKKADKVVALISVDPEVRTRTCIVTSEG
                     HLRLLDHSYFEATARLGRLQHVMRTFKSDPHTIVSAFRYNRNIEELRVYALTDANVIL
                     PLLLTDLAPTPVDKYAKRNINELAEEEKIVSIFNEEIPTISEETKAYAPIVKAEAVKV
                     EPVEDSYEQISIFDLLDED"
     gene            complement(1757..2026)
                     /locus_tag="PLQ55_03705"
     CDS             complement(1757..2026)
                     /locus_tag="PLQ55_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29068.1"
                     /translation="MNLSKLKPYRNMLFLALAAGVGAFMPVIGIIVTVVMYAKRDENS
                     LNFTKEERFLLNALLIILIIYLTLNVLYTLKYPEVKPDTSSETSL"
     gene            2172..>2615
                     /locus_tag="PLQ55_03710"
     CDS             2172..>2615
                     /locus_tag="PLQ55_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HPK29069.1"
                     /translation="MKKFTPNYYAKNIYEVSADFYLKHGIKTLLVDLDNTLASYRELE
                     PSDTTINFVRNLEKAGIKVLLASNNSGKRVKRYGELLGVPYISGARKPFAFKLRKFLK
                     EQGAELSSTLMVGDQILTDVFCGNTLGVKIMLTDKLVEEDQWTTRI"
BASE COUNT          922 a          408 c          526 g          759 t
ORIGIN      
        1 caaacaaaag tcaaatttta cgggaactcg gagcgattga acaaaaccgt aatgttgacg
       61 gtgttgtgga ggtgcgggat gagagtgacc gaaacggaat tcggattgtt attgaactaa
      121 aaaaagaagc gaacactgat gttattttag aatacatctt tagtaaaacg cggttacgcg
      181 ctggctataa cgctaatatt gtcgcaatcg ctgataacag gccaaagacg cttaatattt
      241 tagatttaat tgatgcatat attagtcacc aagttgatgt tattacgcgt cgcagtgaat
      301 ttgatctagc gacaaatgaa aaacgtttac atattattga aggacttatt aaagcaatca
      361 gtgttgtcaa tgaaataatc gcgattattc gtaaaagtac agataaagca aatgcgaaaa
      421 ctaatattaa agcggcctat ggctttagtg aagaacaagc tgaagctatt gtcatgctcc
      481 aactttataa attaagtaat actgatatta ccgtattaat ggaagaaaaa gcggccattg
      541 aaacaacaat tgatgaattg cgtaatatct taagtaatga aaaagcgctt aataacgtta
      601 ttattcgtga tttaaaacgg attgcgcaaa aatttgggga cgaccgtcgt acgaagattc
      661 gtgaaaaagg tgaagtcgtg caaattgata aacgtgactt aatcgccgac gaacaagtaa
      721 tgcttgcgct tacgcgtgac ggttatatta aacgcagtag tttaaaatca tatcggagta
      781 gtggtgactt tgccttacct ggtgttaaaa gtagtgattt acttattgcc gcaagtgaag
      841 taaatacaaa agactttctc gtagcgttta ctgatttagg taattatctt tatataccga
      901 tttatgaaat tggagaagga agatggaaag acgaaggtaa acatattaat tacatcgtcc
      961 aacttaacgc taatgaaaaa attattaaag cgtttaatgt taaagaattt agagaagatt
     1021 tatattatgc aatagcaacc cgtaacggca tgattaaaag aacaagtctt aatgaatttg
     1081 ttgctgttcg ttattcgcgc ccgctccaag ccatgcggct attaagtggt gacagagtag
     1141 ctgatgtcac actgacaacg ggaaatagtg aacttttagt tattactgaa agtggcgctg
     1201 ctagtttcta taatgaaaac gaaattaccg ttgtaagcgt taaagcaagt ggtgttaaat
     1261 cgattaataa aaagaaagcg gataaagtcg tcgcgctcat tagtgttgac ccagaagtca
     1321 gaactagaac atgtattgtc actagtgaag ggcacttacg attacttgat catagctact
     1381 ttgaagcgac cgctcgcctt ggaagattac aacatgtaat gcgcactttt aaaagtgatc
     1441 cacacacaat tgtcagtgcc ttccgttata atcgaaatat tgaagaatta agagtgtatg
     1501 ctttaactga tgcgaatgtt attttaccat tgctcttaac tgacctagcg ccaacgccag
     1561 ttgataaata tgcaaaacgc aatattaatg aactcgccga agaagaaaag attgttagta
     1621 tctttaatga agaaatcccc actatcagcg aagaaacaaa agcttatgcc ccaatcgtaa
     1681 aagcagaggc cgtaaaagta gagccggttg aagactctta tgaacaaatt agtatctttg
     1741 atttattaga tgaagattaa agtgaagttt cactacttgt atctggttta acttcgggat
     1801 attttaaagt atagagcaca ttaagtgtga gatatattat taatataatt aaaagggcat
     1861 ttagtaaaaa acgctcttct tttgtgaaat taagcgagtt ttcatcacgc tttgcataca
     1921 ttacaactgt aacaatgatc ccaatgacgg gcataaaagc accgacacca gcggcaagcg
     1981 ctaagaagag catattacga tacggtttta atttacttaa attcataact aaattgtagc
     2041 acatacgcta acgatttata agaaaagaag gatttaaagg tttaatcttt tataaaaaga
     2101 tttactgttg ttaagcgaga attatatcgt ccatgataaa ataaccttat actttagaag
     2161 tgacagatat tatgaagaaa tttacaccga attattacgc taaaaatata tatgaagtaa
     2221 gtgctgactt ctaccttaag cacgggatta aaaccttact cgttgattta gataacacac
     2281 tagcgagtta tcgtgaatta gaaccaagtg acacaactat taattttgtc cgcaacttag
     2341 aaaaagctgg tattaaagta cttttagcat caaataatag tggtaaacgc gttaaacgat
     2401 atggtgaatt acttggtgtc ccgtatattt caggagctcg taaaccattt gcgtttaagc
     2461 tccgtaaatt ccttaaagag cagggcgcag agttaagtag cacgctgatg gttggagacc
     2521 aaatattaac ggatgtcttt tgcgggaaca ccttaggtgt taaaataatg ttgacagata
     2581 aattagtgga agaagatcaa tggacgacac gtatt
//
LOCUS       DAOPCD010000128         2612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_31010_length_2612_cov_3.709034, whole genome shotgun sequence.
ACCESSION   DAOPCD010000128 DAOPCD010000000
VERSION     DAOPCD010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31010_length_2612_cov_3.709034"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..815
                     /locus_tag="PLQ55_03715"
     CDS             351..815
                     /locus_tag="PLQ55_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPK29070.1"
                     /translation="MTNEHFAIVYETYKVMVFQIAYSYLRNVEDAEDATLESFMRLLR
                     INRKFASGDDERRYLTRIVVNVSKDMLRKKRPVPISDPVIAVNDAGQNENNYIWDEVN
                     KLPNKYKDVIILRYINEHSFNEIASVLKISASAARKRHERALKMLKEELSHE"
     gene            808..1572
                     /locus_tag="PLQ55_03720"
     CDS             808..1572
                     /locus_tag="PLQ55_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29071.1"
                     /translation="MNNHEEKLKKLKNDIPEYPDGLDQKVFRAAEQAPRYKRPGFPKL
                     LIPSLLTVTAVVLSTFFVTKYVYGWNTPSNTPSNSGMPSEPSNTSAEEGEVYDYYVSA
                     SAAIPGASLAPEMLVEGKLGYFDIVYIDSQLALDATINQYADDAHPVLSTLTSVIRNN
                     AEFFSESVLIIVPFAHTSTEEGIELTQIEFGSKEQPTKLRFDIYSPEFNDMDTLVTFH
                     LVTIGEWASDLLSESAFKISVHNTFDNSNKSVFYSN"
     gene            1583..2611
                     /locus_tag="PLQ55_03725"
     CDS             1583..2611
                     /locus_tag="PLQ55_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29072.1"
                     /translation="MKRTQILVAGLLLILTSCRAANHQTLSEVFPMLKTEEFAEVKHT
                     ISCNSLAPLALINHYYNDDVAAVTNFVNELGKTKVRRYAKLPESKNVACPTINTYAFK
                     NEALEFTFDVTNMFVVKDDVYYELEELPNIVTTYSSYSFSGSTTITEVFNSEGKEITD
                     FSLSLPSLEFKELTELSGNNVNFTIETVGGIISVYDAVTFGLNVGEQIERFYEVISGD
                     TFANLFDTNNNFTDDTMLLANIPQQLTFRNQKYKMITSGFFEWQDIQLDLLIAYFVNE
                     RDYDVFYLENPDIEYCIDEANTLYYAAGPSKFPLYSLVGHAIEQLLRLFLPQGGGSPL
                     IFLNISCV"
BASE COUNT          883 a          444 c          498 g          787 t
ORIGIN      
        1 tatttaccag tatatatgac aatatttctt taaaacgaag gttcgttaac ataacgagag
       61 tagtatttta caaaatctta gttattttaa gtttcgcatc gataaaactt taaaaatcaa
      121 tttttttcta gctataaatc acacttaaaa ataatattta ttggaaaaag tgtaaactta
      181 agataaggat ttttttaaga gtttttaaca aaaaaagttt ttgtcacaaa acacgctttg
      241 taatcgttat gtattatagt agagaaataa ttagtactaa tagagaggag caaattgtac
      301 ttaatgtcac aaaatgacgt taagttacgt ttgtattatg taagaagaaa atgactaacg
      361 aacattttgc gatcgtttat gaaacttaca aagtgatggt tttccaaatt gcttactctt
      421 acttacgaaa tgtagaagat gcggaagacg caacgcttga gtcatttatg cgcctcttac
      481 gaataaaccg aaaatttgca tcaggtgacg acgaacgacg ctacttaaca agaatcgttg
      541 ttaacgtaag taaagacatg ttacgcaaga aaagaccagt gccaattagt gatcccgtga
      601 ttgcagttaa tgacgcaggg caaaatgaaa acaattatat ttgggacgaa gttaacaaac
      661 ttcctaataa atataaagat gtgattatct tacgttacat caatgaacat agtttcaatg
      721 aaattgcgag tgtgctaaaa ataagtgcaa gtgccgcaag aaagcgtcat gaacgagcgc
      781 ttaaaatgtt gaaggaggag cttagtcatg aataaccatg aagagaaact aaaaaagctt
      841 aaaaatgata tccccgagta tcccgatggt ttagaccaaa aagttttcag ggccgctgaa
      901 caagcaccac gctacaagcg acccggcttt cctaaactac ttattccctc cttgctaacc
      961 gtaacagcag tcgtgttatc aactttcttt gtcacaaagt atgtttatgg ctggaacacg
     1021 ccttcaaata ccccaagtaa ttcaggaatg ccaagtgaac catctaatac cagtgcagaa
     1081 gaaggggaag tttatgatta ttatgtttcg gcaagcgcag caataccggg tgcgtccctt
     1141 gccccagaaa tgcttgttga agggaaacta ggctattttg atatcgttta tattgattca
     1201 cagcttgcgc tagatgcaac aattaatcaa tatgctgatg acgcacaccc agtacttagt
     1261 acattaacga gtgtaattag aaataacgcg gaattcttta gtgaaagcgt cttgataatt
     1321 gtcccgtttg cccatacaag tacagaagaa ggtattgaac ttacacaaat cgaatttggg
     1381 agtaaggagc aaccaacaaa actgcgtttc gatatatatt caccggaatt taatgatatg
     1441 gatacccttg tgacgtttca tcttgtcacg ataggtgaat gggcaagtga tctattaagt
     1501 gaaagtgcgt ttaaaataag tgtacataat acgtttgaca atagtaataa atcagttttt
     1561 tatagtaatt aatggaggga aaatgaaacg aacgcaaatt ttagtcgccg gcttactctt
     1621 gatattaaca tcatgcagag cggctaatca ccaaacactt agtgaagttt tcccaatgct
     1681 taaaacagag gaatttgctg aagttaaaca taccattagt tgtaatagtc ttgcaccttt
     1741 agcgcttatt aatcattact ataacgatga cgtagccgcc gtaacgaact ttgttaatga
     1801 actagggaaa actaaagtgc gtcgttacgc aaaacttcct gaaagtaaaa atgttgcgtg
     1861 cccgacaatt aatacgtacg cttttaagaa tgaagctctc gagtttacgt tcgatgtcac
     1921 aaatatgttc gttgtgaaag atgatgtgta ttatgaacta gaagaattac caaatattgt
     1981 cactacatac agctcgtata gttttagcgg ttccacaacg attacggaag ttttcaatag
     2041 cgagggaaaa gaaataacgg atttttcgct ttccttgcct tcactagaat ttaaagaact
     2101 aacggagctt agtggaaata atgtaaactt cacgattgaa acagtaggcg ggattatttc
     2161 tgtgtatgac gcagttactt ttggccttaa tgttggggaa caaatagagc ggttttatga
     2221 ggttataagt ggtgacacat tcgcaaactt atttgacact aacaataatt ttacagatga
     2281 cacaatgctt ttagcgaaca tcccccaaca acttactttt agaaatcaaa aatacaaaat
     2341 gatcaccagt ggttttttcg aatggcaaga cattcaacta gatttgctaa ttgcttattt
     2401 cgttaatgaa cgtgactatg atgtgtttta tttagaaaat cccgatatcg aatattgtat
     2461 tgatgaggcg aatacacttt attatgctgc tggcccaagt aaatttccgc tttattcgct
     2521 cgttggtcat gctatcgagc aattacttcg cttatttctc ccgcaaggcg gaggttcgcc
     2581 attgatattt cttaatataa gttgcgtcta gt
//
LOCUS       DAOPCD010000129         2603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_31141_length_2603_cov_3.678964, whole genome shotgun sequence.
ACCESSION   DAOPCD010000129 DAOPCD010000000
VERSION     DAOPCD010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31141_length_2603_cov_3.678964"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PLQ55_03730"
     CDS             <1..1002
                     /locus_tag="PLQ55_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPK29073.1"
                     /translation="LIVIGAYFLVLFLYSLLIYIFTSVRSKMRCDKNPLFTYYTYEDY
                     QGLKAETFNFKNFKGDNLMARMYTRDGFKDDTYLVFFHGIGAGHEAYTTLINDLVTTL
                     EMPLLTFDYTGCDFSEGKSIVSVYQPLVDAHAFLAHLQTMPQYKGKKLVLIGHSWGGF
                     VAANLYPFNKDKNIIKVVNLNGVTDFGLYIKHSAKAPFIFVPLFKLFNAFRYGKFAFA
                     ATRNSIKNTQIPHLIVHGEKDSVVDFNLFIGNLVLQEDKHKNIKFHFESDKNHSAYLT
                     TDGEKALGEVMGYFLTYSKQKNKDETLKQKIVDFDFSRAVENDPKTLEVIRRFVKGD"
     gene            1005..>2603
                     /locus_tag="PLQ55_03735"
     CDS             1005..>2603
                     /locus_tag="PLQ55_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPK29074.1"
                     /translation="MVNEELKTLLHTLSLTTKLKLLTGANFWESEKVLGTDLPIIVMN
                     DGPFGVRKPTVNVDSGMLMEVIPATAFPTTSALASSFDPELLEKMGKTLATECIDQGV
                     DLLLGPGINIKRSPLCGRNFEYFSEDPYLTSEMASGLVRGLQSKGVGATLKHYAVNNQ
                     EKERYTTNAIVDKRALNEIYLRPFHNVIKNTEPFSVMCSYNQINGRHASRHYKLLTEK
                     LRNEANFKGLVVSDWGAVVDHVDSINAGLNLEMPGGYQNTAELEKAVKDGEIKLETID
                     EMITPLLKFMMYKRDNPKPKVKCDYKKNLEVAQEIAEKSAVLLRNEDNLLPINKTAKV
                     GLIGNFAIKPRYQGSGSSQINPHTLVSVHDAFIQNDVNFVYADGYAEHEVKPNKKKIA
                     EAVKVAKNSDVVVLCVGLPESYEVEGYDRKTLDMPPSHNELIKEVAKVNENIVIVLST
                     GSPVTMPWINDVKSILLTHLAGSMTGPATYNLLFGVTNPSGKLSETYPLSLDKGLPGG
                     EFLTSDQNVLYRESLYVGYRYYDTF"
BASE COUNT          870 a          403 c          516 g          814 t
ORIGIN      
        1 ttaatcgtta ttggagctta ttttttagtt ctttttcttt attcactatt aatttatatt
       61 ttcactagtg ttcgctcgaa gatgcgatgt gataaaaacc cgctatttac ttattacact
      121 tatgaagatt atcagggact taaagcggaa acatttaatt ttaaaaactt caaaggcgat
      181 aacttaatgg ccagaatgta tacacgggac ggatttaaag acgatactta tcttgtattt
      241 tttcacggga ttggggcagg gcatgaagct tatacaacac tgattaatga tttagtgacc
      301 acattagaaa tgccactttt aacatttgat tacactggtt gtgattttag cgaaggtaaa
      361 agcattgtta gtgtgtatca accgcttgtt gacgctcatg cttttttagc gcacttacaa
      421 acaatgccgc aatataaagg taaaaaactt gtcttaattg gtcactcgtg gggtgggttt
      481 gttgcggcaa atctttatcc atttaataaa gacaaaaata ttattaaagt tgttaacctt
      541 aatggtgtca cagattttgg gttatacatc aagcattcag caaaagctcc atttatcttt
      601 gtgccactct ttaagttatt taatgctttt agatatggca agtttgcgtt tgccgcgaca
      661 cggaacagta ttaaaaatac acaaatcccg catttaattg tgcatggtga aaaagattcc
      721 gttgttgatt ttaatctctt tatcggtaac ttagtgttac aagaagataa acataaaaac
      781 atcaaattcc actttgaaag cgataaaaat cactctgcgt acttaacgac tgatggcgaa
      841 aaagcacttg gtgaggtgat gggatatttt cttacatata gtaagcaaaa aaataaagac
      901 gagacattaa aacaaaagat tgttgatttt gatttttcac gcgcagtgga aaatgatcca
      961 aaaacacttg aagttattag acgctttgta aagggggact aattatggtt aatgaagaat
     1021 taaaaacctt gttacacacc ttatcactta cgacaaaatt aaaactttta acgggcgcta
     1081 atttttggga aagtgaaaaa gttctgggca ctgatttacc aataattgta atgaatgacg
     1141 gaccttttgg tgtacgaaaa ccaacagtta atgttgattc tggaatgctc atggaagtaa
     1201 ttcctgcaac ggcttttcca acaacgagtg cactcgctag ttcttttgat cctgaactat
     1261 tagagaaaat ggggaagaca cttgctactg aatgtattga tcaaggtgtt gaccttttgc
     1321 ttggtcctgg tattaacatt aaaagaagtc cgctctgtgg acgaaacttt gaatatttta
     1381 gtgaagaccc atatttaact agcgaaatgg ctagcggtct agttcgtggg ctacaaagta
     1441 agggagttgg ggctacacta aaacattatg ctgttaataa tcaagaaaaa gaacgctata
     1501 cgactaacgc cattgttgat aaaagagcat taaatgaaat atatttaaga ccgttccaca
     1561 atgttattaa aaacactgaa cctttttcag ttatgtgttc atataaccaa atcaatggtc
     1621 gacatgcgag tcgccattat aaattactca cagaaaagtt acgcaacgaa gcaaacttta
     1681 aaggtcttgt cgttagtgac tggggtgcag tagtggatca tgttgatagt attaacgctg
     1741 gtcttaactt agaaatgcct ggcggttatc aaaataccgc tgaactagaa aaggcggtta
     1801 aagacggcga aattaaatta gaaacaattg atgaaatgat taccccgctt ttaaaattta
     1861 tgatgtataa gcgtgataat cctaaaccaa aagtcaaatg cgattataag aaaaaccttg
     1921 aagtagcgca agaaattgcc gaaaagagtg ctgttttgct gcgaaatgaa gataatttgt
     1981 taccaattaa taaaacggca aaagtaggat taattgggaa ttttgcaatt aagccgcgtt
     2041 atcaaggcag tggttcaagt caaataaatc cgcatacttt agtaagtgtt catgatgctt
     2101 ttatacaaaa tgatgttaat tttgtgtacg ctgatggtta tgcagagcat gaagtaaaac
     2161 caaataaaaa gaaaatcgcc gaagcagtta aggttgcgaa aaatagtgat gttgttgttt
     2221 tatgtgttgg tttgcctgaa agttatgaag tggaaggcta tgaccgtaaa acattagaca
     2281 tgccaccgag ccataatgaa ttaataaaag aagttgcaaa agttaatgaa aatattgtca
     2341 ttgtcttatc aactggttcg ccagttacaa tgccgtggat caatgatgta aaaagtatct
     2401 tacttaccca cttagcgggt agtatgacgg gaccagcgac atataattta ctgtttggtg
     2461 ttactaaccc ctctggtaag ttaagtgaaa cttatccgtt aagtttagat aaaggattac
     2521 ctggtggaga gtttctcact agtgatcaaa atgttttata tcgtgaatca ctttatgttg
     2581 gctatcgtta ttacgatact ttt
//
LOCUS       DAOPCD010000130         2583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_31477_length_2583_cov_3.554193, whole genome shotgun sequence.
ACCESSION   DAOPCD010000130 DAOPCD010000000
VERSION     DAOPCD010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31477_length_2583_cov_3.554193"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..1404
                     /gene="mutM"
                     /locus_tag="PLQ55_03740"
     CDS             31..1404
                     /gene="mutM"
                     /locus_tag="PLQ55_03740"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HPK29075.1"
                     /translation="MPELPEVETVKKILNKVIVGAKITDVIIHKENIITGDPHTFKKT
                     LIGETFSEVKRIGKYLIFTFESDLIMLSHLRMEGKYIEVLPGTEDSRYARVIFVLNDG
                     RRLCYDDSRQFGTLELSTKDTYLQTKSMQNVGREPFDIDPIDYFNKISNRTTPIKQTL
                     LDQKLMSGLGNIYVDEVLYLSKIHPETPTNMLGLMAINLLIENSIKVLNAAIKKGGTT
                     VRSYSPAEGVHGNFQQELNAYGKAGERCPVCNFKFKKIFVGGRGTTYCPSCQKNPAVP
                     LTVAITGQKASGKTLIANYLRKNGHKVIDTDKLAKELYKDIKITTDLEKHLGVNLHDN
                     TTFNLQYLRNYLVENENEIKKLNDFIHPLVKKEIIEIIKTSNEAILYFEVPLLFSHKI
                     YELFNFMIGVEVSVEKQLKNLEKRGDTHTINADEAYMKNRHKLDLIIVNDGSEQDLIE
                     KFKAFKV"
     gene            complement(1394..2272)
                     /locus_tag="PLQ55_03745"
     CDS             complement(1394..2272)
                     /locus_tag="PLQ55_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29076.1"
                     /translation="MKKVELDIDTTNVEEIRQEMYDKIMSHKSFVKLLEEKGATPEII
                     KNNVGLLNDYLESYLSVEKCRKAGHCVSDEKHHALTIQIDAPFVTVERHLCPVYLESQ
                     SLAMRFIVRDFPENYLDLRVEDLPARRGFAAFIRELMKVLKGDIYFIYAYGTAGIGKL
                     KTTIPFVTKMLEDDKNKTAGVVNFPAFVRASIADYYDKKDIVDSYLQHYIDLDVLVLD
                     NFGNEEVNKIVIESIIFPLLAARVRQKKPTIILAGLSTNDLRSLYVGRSVRGHQIVDL
                     IKANIKEEIYLQGTKL"
     gene            complement(2269..>2583)
                     /locus_tag="PLQ55_03750"
     CDS             complement(2269..>2583)
                     /locus_tag="PLQ55_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29077.1"
                     /translation="YNILTTLSHDYNLLSPVINALVYYTLYHNNNELIRALVEKNASL
                     LARNKVTTALDALDTLERPIKKRGRAPKKAPILNDDKKEEATPQVDDLDDPIWDELKK
                     LK"
BASE COUNT          884 a          431 c          437 g          831 t
ORIGIN      
        1 aaaaacatgg tttgaagcga gttaataatt atgccagaac taccagaagt tgaaacagtt
       61 aaaaaaatct taaataaagt cattgttggg gcaaaaatta ctgatgttat tattcataaa
      121 gaaaacatca tcactgggga cccacacact tttaaaaaga cacttattgg tgaaactttt
      181 agtgaagtaa aacgcattgg taaatactta atctttacct ttgaaagtga tttaattatg
      241 cttagtcact tacggatgga aggtaagtat attgaagttt taccgggtac tgaagattcg
      301 cgttacgctc gtgtaatttt tgtgttaaac gatggcaggc gcttgtgtta tgacgattca
      361 cggcaatttg gtacgcttga attatcaact aaagacactt acttacaaac aaaatcaatg
      421 caaaatgtag gaagagaacc ctttgatatt gatccgatcg attattttaa taaaataagt
      481 aaccgcacta caccaattaa acaaacttta cttgaccaaa agctgatgag cgggctcggt
      541 aatatttatg ttgatgaagt attatactta agtaaaattc atccagaaac accaacgaat
      601 atgttaggac ttatggcaat aaatttactt atcgaaaata gtattaaagt acttaacgcg
      661 gccataaaaa aaggcggcac aaccgtaaga agctatagtc ctgctgaagg agtgcacggt
      721 aacttccagc aagaattaaa tgcttatggt aaggcgggtg aaaggtgtcc tgtttgtaat
      781 tttaaattca agaaaatatt tgttggtggg cgcggtacaa cttattgtcc aagttgtcaa
      841 aaaaatcccg ctgtgccatt aactgtcgcc attactggtc aaaaagcaag tgggaaaaca
      901 cttatcgcta attacttacg gaaaaatgga cacaaagtta ttgacactga taaactcgct
      961 aaagagcttt ataaagatat taaaattaca actgatttag aaaaacatct cggcgttaat
     1021 ttacatgata acactacttt taaccttcaa tatttacgaa attatctagt ggaaaatgaa
     1081 aatgaaataa aaaaactaaa tgactttatt catccgcttg taaaaaaaga aattattgaa
     1141 attattaaga cgagtaatga agcgattctt tattttgaag taccgcttct tttctcgcat
     1201 aaaatatatg aattatttaa ttttatgatt ggggttgaag taagcgttga aaagcaactt
     1261 aagaatttag aaaagcgcgg agatacgcat acaattaacg ctgatgaagc atatatgaaa
     1321 aaccgtcata aactagactt aatcatcgtt aatgacggta gtgaacaaga tttaatcgaa
     1381 aagtttaaag cttttaaagt ttagtgcctt gtaaataaat ttcttcttta atattggctt
     1441 taattaagtc aacaatttga tggccacgga cactgcgacc aacataaaga cttcgtaagt
     1501 cattcgtgct tagacccgct aaaataatag tgggcttttt ttgtctgact ctcgctgcta
     1561 gaagtggaaa aataattgat tcaatgacaa tcttattaac ttcttcatta ccgaagttat
     1621 caagcactaa aacatctaaa tcaatataat gttgtaaata agaatcaacg atatcttttt
     1681 tatcgtagta atcagcaatt gatgctctaa caaaagcagg gaaattgaca actcccgctg
     1741 ttttattttt atcatcttca agcattttag tcacaaacgg aatcgttgtt tttaatttac
     1801 caatccccgc tgtaccataa gcatatataa agtagatatc accttttaaa actttcataa
     1861 gttcacgaat aaaagctgca aagcctcgtc tagcgggtaa gtcttcgact ctcaaatcca
     1921 aataattttc gggaaaatca cgaacaataa agcgcatcgc taatgattga ctttctagat
     1981 aaactgggca taaatgtctt tccactgtca caaacggcgc gtcaatttga atagtgagtg
     2041 catgatgctt ttcatcacta acgcagtgac cagctttacg acatttttct acacttaagt
     2101 aactttctaa ataatcattt aaaagtccaa cattgttctt aattatttct ggagttgcac
     2161 ctttttcttc taaaagttta acgaaagatt tatgactcat aattttgtca tacatttctt
     2221 ggcgtatttc ttcaacattt gtggtgtcaa tatctaattc tacttttttc attttaactt
     2281 cttcaattca tcccaaattg gatcatcaag atcatcaact tgcggtgttg cttcttcttt
     2341 cttgtcatcg ttaaggattg gcgctttttt aggtgcgcgt ccgcgttttt ttatcggccg
     2401 ttcaagtgta tcaagggcat caagcgcggt agttactttg tttctcgcta gtaaactagc
     2461 gtttttctcc actaaagcac ggataagttc attgttatta tgataaagcg tgtaataaac
     2521 aagggcatta attactggtg ataataaatt ataatcatga cttagcgtcg ttaaaatatt
     2581 ata
//
LOCUS       DAOPCD010000131         2567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_31732_length_2567_cov_2.835987, whole genome shotgun sequence.
ACCESSION   DAOPCD010000131 DAOPCD010000000
VERSION     DAOPCD010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31732_length_2567_cov_2.835987"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PLQ55_03755"
     CDS             complement(<1..803)
                     /locus_tag="PLQ55_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPK29078.1"
                     /translation="MLTRVIGLDIGAATMRAAVVDREGTIYYSKEVATPERSELRFVR
                     EINDLLENVLFHAPEAIAIGVGVPGIVSPGTTLIHLLPNLGYENIDFKRILRKEIKQP
                     VYLLNDANAACYAEALFGAGADKRIVQYITLSTGVGGGLVINKEIYTGARGFALEIGN
                     MIIDTETPRPNDKMNRNSFEGSCSGKSLYNQAKRFGTNIHTVASVFNESRFSPIRNVW
                     VDNLGKGIANIYSLYDPDVVILGGAVMQSADKFFDDILPAVEKYTLPQL"
     gene            1554..1721
                     /locus_tag="PLQ55_03760"
     CDS             1554..1721
                     /locus_tag="PLQ55_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29079.1"
                     /translation="MSVVLLALLKNFAVMPNVDEPAKALEAYKLTGGAVIALVLAGLG
                     GVAGVAADLLK"
     gene            complement(1808..>2567)
                     /locus_tag="PLQ55_03765"
     CDS             complement(1808..>2567)
                     /locus_tag="PLQ55_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPK29080.1"
                     /translation="NNELTKANYRPMVFFESSQDGSLSLEVAREIISMRIVGVITYLV
                     PSKEVIELFEDHNVPIVLLGRTGDALGIDSVASNDYRGGKLAGYKLFDLGGKNFAYIG
                     VTRKLNINNERLNGFLKALKNNDVIVPEANIILNEKQQTTRQLMDTLDVQKKKIDSIF
                     CFNDQIAYEVIGYLSEKGIKVPEDINIIGFDNLQSAIIYPIKLTTIDADKPRAAHLAL
                     VSLFSKIKDSEDTEVTGKIVDVTLVEGNTTKMRK"
BASE COUNT          771 a          466 c          444 g          886 t
ORIGIN      
        1 tgaagttgcg gtagtgtata cttttcgacc gctggtaaaa tatcatcaaa gaatttatcc
       61 gcactttgca tcacagcacc accaagaatt acaacatcag gatcatagag tgaataaata
      121 ttagcgatac ctttccctaa gttatcaacc cacacatttc taatcgggga gaaacggctt
      181 tcgttaaaca cacttgcgac ggtatgaata ttcgtgccaa agcgttttgc ttggttatat
      241 aaacttttac ccgaacaact cccttcaaaa ctattacgat tcattttgtc attagggcgc
      301 ggtgtttctg tatcaataat catattgccg atttctaaag caaagccacg tgctcctgta
      361 taaatttctt tgttaataac aagtccgcca ccaacaccgg tcgataaagt aatatattgg
      421 acgatgcgtt tatcagcacc ggcaccaaaa agtgcttcag cataacacgc tgcgttagcg
      481 tcgttaagta aatagacagg ttgctttatt tctttacgga gtattcgttt aaagtcaata
      541 ttttcgtaac ctaagtttgg taagagatga attaatgtag tgccggggct aacaatacca
      601 ggaacaccaa caccgatggc gattgcttca ggggcatgaa ataaaacatt ttctaataaa
      661 tcatttattt ctcgcacaaa gcgtaattca ctgcgttctg gtgttgcaac ttccttacta
      721 taataaattg ttccctcacg gtccacaacc gcggcacgca tagtagcggc accgatatct
      781 aatccaataa ctcgtgttaa catattaatt tgtcctttct aaattcatta ttactactat
      841 taattcaaca ctaaagaaat gtaagagtag taactatgaa tttctttcct tatgaataat
      901 tcatcgtaat aattttatca taattgtgcc ttagtgatgg taaagggtta tgcgaagcat
      961 tcataatctt tacatttagt cttgaattac taatattagc actaaattaa aagcaagata
     1021 aagtccttta ttttaaggtg aaaagtgtga acaaaacggg gctttgttca ttactaattg
     1081 taaaatattt tttaatatca cttttccaaa gagaagtact taataactta tgcccattat
     1141 acgctaatta ttcggtttta agtgcttatt tactaaatga acaaccgttt attattttgt
     1201 gattttggaa agttaatagt tgcctaactt tatattttat gcaaaagaaa aaattattaa
     1261 atgtagtcgc agctggttta tttttagttg ccctcatctg atggtttgcc gttcctgcga
     1321 ttgctagaac tcaaccagaa gtaagttaca ccttccttga tttagccttt ggaagaactg
     1381 aaacgctcct tggagtagaa gttatagaag ttattattct taagtttagt ttcttaaact
     1441 tcttagtctt attacttctc cttgctgggg ttggacttag tgttggccgt ttagtcgtta
     1501 aaagtttaga aaaaaataaa cttcttggct ttgtcatttt agcgcttggc ttagtgagtg
     1561 ttgtattact agcacttctt aaaaactttg cggttatgcc aaatgttgat gaaccagcaa
     1621 aagcactaga agcttataaa cttactggtg gtgccgtcat cgctttagtc cttgctggac
     1681 ttggtggtgt tgctggtgtt gccgctgatc ttttaaaata agattaattt ttaattaaaa
     1741 tgaaagataa cgcttcgtcg cgttatcttt ttttatattt ttattaatta tttgcggcaa
     1801 aaaggactta ttttcgcatt ttagttgtgt taccttcaac aagtgtaaca tcaactattt
     1861 ttccagttac ttcagtatct tcactatctt taattttaga gaataaactc acgagcgcta
     1921 aatgggcggc acgcggttta tcggcgtcaa ttgttgttaa tttaattgga taaataattg
     1981 cgctttgtaa attatcaaag ccgataatat taatatcttc aggtactttt attccttttt
     2041 cacttaagta gccaattact tcgtaggcta tttggtcatt gaaacaaaaa atggaatcaa
     2101 tctttttctt ttgcacatca agcgtgtcca ttagttgacg ggttgtttgt tgcttttcgt
     2161 taagaataat gtttgcttca ggaacgataa catcattatt tttaagtgct tttaagaacc
     2221 cgttaagtcg ctcattatta atattaagct tgcgggtcac accaatgtag gcaaagtttt
     2281 taccacctaa atcaaataat ttataacctg cgagtttacc accacgataa tcgtttgacg
     2341 caacgctatc aatcccgagt gcatccccag tgcgtcctag gagtacaatg gggacattgt
     2401 ggtcttcaaa aagctcaata acttctttac ttggcactaa ataagtaata acaccgacaa
     2461 tgcgcattga gatgatttca cgggcaactt caagtgataa agaaccgtct tgactacttt
     2521 caaaaaatac cattggccgg taattggcct ttgttaattc attatta
//
LOCUS       DAOPCD010000132         2565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_31764_length_2565_cov_2.521514, whole genome shotgun sequence.
ACCESSION   DAOPCD010000132 DAOPCD010000000
VERSION     DAOPCD010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31764_length_2565_cov_2.521514"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..>555)
                     /locus_tag="PLQ55_03770"
     CDS             complement(129..>555)
                     /locus_tag="PLQ55_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29081.1"
                     /translation="VVYQMLVYPEARTFFDNHVRVFTHEVHRYIVNYLKEFKPKEQVN
                     YALLLNDIQSRFTDEATINEYTQELLTIESGLQDVKYDENILNDAAKTLNYERELQIL
                     ERKHQDELLRAPDEASYAALKVKHIQAKTELMQKYGKKK"
     assembly_gap    556..565
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<566..1865)
                     /gene="dnaG"
                     /locus_tag="PLQ55_03775"
     CDS             complement(<566..1865)
                     /gene="dnaG"
                     /locus_tag="PLQ55_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPK29082.1"
                     /translation="MPLHKQGQNYSALCPFHDDRNPSLMISPSKQIFNCFVCNTGGNA
                     IRFVALYEKKTYYEAAQRLADMVSFKHDFFTKKQKFVVRDTKLDPLISAINDLTAYYE
                     YTLITEAGSEAKKYLVNRGLDDALLKKYKIGFAPRDGEATIKFLQSKGHALKTIEDIG
                     ILGGSLERPYDINHGRIIFPLNNKDGQVVAYSARALPSEKKAPKYVNTRETQLFIKSD
                     TLYNYHHAKALMAAQYVYLVEGFLDVIALDRVGERSVVALMGTAFTKEQLALLRLLNK
                     EVRIYLDSDTAGQMATLSVIKDLIAANIPLQIVKPHKDGYDPDDILNKEGKEALVTAL
                     TTFIPRDDFIFNYYLSVTKLDDMASTKKFVNDVMNEIILKLNSRLEVSAMIERLAEAS
                     GFNSLVLTDMYESLRRKKQTRNEAINTVKQKLPVRQTLNKV"
     gene            complement(1966..>2565)
                     /locus_tag="PLQ55_03780"
     CDS             complement(1966..>2565)
                     /locus_tag="PLQ55_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPK29083.1"
                     /translation="DHTKDELSFYSLATTDIEYLFPFGWGEIWGIADRTDYDLKQHME
                     HSGENFEYLDPDTNERYIPYCVEPSVGLDRLLLMTLVDSYDEEELEKDTRIVMRLAPH
                     LAPYQVAVLPLQRKLSEKALAVFSDLVKDFSTVYDETASIGKRYRRQDAIGTPYCVTI
                     DFDTLEDESVTIRERDSMAQIRLPLSELKAYLHSKVKFQ"
BASE COUNT          723 a          522 c          459 g          851 t
ORIGIN      
        1 agtagaaact tccgaatctg cggtaatgtt aactcaattt ccacttcatc atcttcttca
       61 atcgctactt cctctaattc agcgaggtct tttaatttgt cttcatccat aaactatcgt
      121 ctcctttact atttcttctt tccatatttt tgcattaatt cagttttagc ttgaatgtgc
      181 ttaactttaa gggcggcata actcgcttca tcaggcgcac gtaagagttc gtcctgatgt
      241 ttgcgttcta agatttgtaa ttcgcgttca taattaagcg tcttagcggc gtcatttaat
      301 atattttcat cgtatttcac atcttgcagg ccactttcaa tcgtgagtag ctcttgcgta
      361 tattcattaa tagtagcttc atcagtgaaa cgtgactgaa tatcatttaa taaaagcgca
      421 taattaactt gctcttttgg tttaaattct tttaagtaat taacgatata gcggtgtact
      481 tcatgcgtaa acacgcgtac atggttatca aagaaagtgc gtgcttcggg atacacaagc
      541 atttgataaa caacannnnn nnnnncgact ttatttaatg tttgccgcac cggtaacttt
      601 tgtttaacag tatttatcgc ttcgtttctt gtttgttttt tacgccgtaa gctttcatac
      661 atatcagtga gcactaaact attaaagccg cttgcttcgg caagtctttc aatcatcgca
      721 cttacttcaa gacggctatt tagttttaag atgatttcat tcataacatc attaacaaac
      781 ttctttgtac tagccatatc atcaagcttt gtaactgata agtaataatt aaaaataaag
      841 tcatcacgcg gaataaaggt tgttaaggct gttacaagcg cttctttccc ttctttattt
      901 aatatgtcat caggatcata gccgtcttta tgtggtttta cgatttgcag cggtatattc
      961 gctgcgatta aatctttaat cacacttagc gtcgccattt gaccagcagt gtcactatct
     1021 aaataaattc ttacttcctt attaagtaag cgtaaaagcg ctaattgttc tttcgtaaat
     1081 gctgtcccca ttaaggctac aacactgcgt tcaccaacgc ggtcaagagc aatcacatca
     1141 agaaaccctt cgacgagata cacatattgc gctgccatca gtgcttttgc gtgatggtag
     1201 ttataaagtg tatccgactt aataaataat tgtgtctcac gcgtattaac atattttggt
     1261 gctttctttt cgctaggcaa cgctcttgcg ctataagcga caacttgtcc gtctttatta
     1321 ttaagcggga aaataattcg cccgtgatta atatcataag gtcgctcaag actaccacct
     1381 aaaataccaa tatcttcgat cgttttaagg gcgtgacctt tactttgtaa aaacttaata
     1441 gtcgcttcac cgtcgcgcgg ggcaaaccca atcttatatt ttttaagtaa ggcatcatca
     1501 agcccgcgat taacgagata ctttttagct tcactacctg cttccgtaat taaggtatac
     1561 tcgtaataag cagttaaatc attaatcgca ctgataagcg ggtcaagctt tgtatcgcgc
     1621 acaacaaact tttgtttttt tgtaaaaaag tcatgcttaa aactaaccat atccgcgagc
     1681 cgttgagcag cttcatagta tgtctttttt tcgtaaagcg ctacaaagcg aatcgcatta
     1741 ccgccggtat tacatacgaa gcaattaaag atttgctttg atggcgaaat cattagacta
     1801 ggattacggt catcgtgaaa tggacatagt gcactatagt tttggccttg tttatggagc
     1861 ggaatataac ggccaatgac atcaactatg tcaacatttt cgattaatag atcataaggg
     1921 ctgcgagtac taataagcta aacctcctaa attactacta attaattact gaaattttac
     1981 ttttgagtgt aaataagctt ttaactcact taatggtaag cgaatctgtg ccattgagtc
     2041 gcgttcacga attgtgacgc tttcatcttc taaagtgtca aagtcaatcg tgacgcagta
     2101 gggtgtaccg attgcatctt gacggcgata gcgtttacca attgaggcgg tttcgtcata
     2161 gacagtacta aagtctttta ctaagtcact gaagactgca agtgcttttt cacttaactt
     2221 ccgttgtaag ggtaataccg ctacttgata aggggctaag tgcggggcaa ggcgcataac
     2281 aatccgggta tctttttcta gttcttcttc gtcataacta tcaacgagag tcatgagtaa
     2341 taagcgatca agcccaacac taggttcgac acaatatggg atatagcgct cattcgtatc
     2401 tgggtctaaa tattcaaagt tttcaccaga atgttccata tgttgcttta agtcgtaatc
     2461 tgtgcggtca gcaatccccc agatttcacc ccagccaaat gggaataagt attcaatatc
     2521 ggttgttgct aaactataaa agcttagttc atctttcgtg tggtc
//
LOCUS       DAOPCD010000133         2562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_31800_length_2562_cov_4.619864, whole genome shotgun sequence.
ACCESSION   DAOPCD010000133 DAOPCD010000000
VERSION     DAOPCD010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31800_length_2562_cov_4.619864"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PLQ55_03785"
     CDS             <1..737
                     /locus_tag="PLQ55_03785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPK29084.1"
                     /translation="IDDEEFRKQFHNLKNLKSGSPSILDFEEAINAKFDEGYKEVIVL
                     PLASGLSATFNIAVMAREGLSPERKAATYIHESNNASIGMDLLMHSLVELLDTDPTAD
                     ELLAALEARAPQNRVLFEIDNLRHLFRGGRLNPLKYIIGTALKIKPLVEFKDGELKVI
                     AQNRSRRKTMDVILGKIDDFVQNFKNVYVGLFTYDKEDNIFKAIHDIIKERWPHIRIN
                     FSKKICPIFTTHVGVDGYSVAVVSYN"
     gene            747..1073
                     /locus_tag="PLQ55_03790"
     CDS             747..1073
                     /locus_tag="PLQ55_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29085.1"
                     /translation="MAKRKIDGGELALYIIYGVIALGGLTLVVLHLIGMNLANLENAL
                     RVADETFAEKMKMDFLVFGSLLVVLAGALSAITLAIYGNRAELEEEKRARRRQRMALE
                     DFSDLE"
     gene            complement(1102..1977)
                     /locus_tag="PLQ55_03795"
     CDS             complement(1102..1977)
                     /locus_tag="PLQ55_03795"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HPK29086.1"
                     /translation="MHKDKIINAFKIYLNKDEVNLEYQERFLGGMSNYTYHVKVNGKD
                     YVIRIANKEGKVFVDYATEKLHLFLLEPFNITSKTLFYDTDTGIKISEYIPGDNLTAT
                     LSAEDYAGVARALHKLHALNIEGVDYGQIDRLARYESLVEKHLTPEYFELKAFWMNEN
                     STTYKDADIVFTHGDAQRTNIVKNGDSYTLVDFEFAGMNDRYFDIASFGNINFSDSLN
                     LLDYYLGRKALPHEVRRVKFHRLFQVLQWHVVALQKHLNGQSEKLLIDFKKYSDNYLA
                     FAAYLATKIKALPIK"
     gene            2026..2101
                     /locus_tag="PLQ55_03800"
     tRNA            2026..2101
                     /locus_tag="PLQ55_03800"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2059..2061,aa:Arg,seq:cct)
     gene            2110..2463
                     /locus_tag="PLQ55_03805"
     CDS             2110..2463
                     /locus_tag="PLQ55_03805"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HPK29087.1"
                     /translation="MKRHEFTLKPIPFMRIKNKQKVIEMRLYDEKRQKLRVGDFIYFT
                     NTETKEILKVEILELTRYLNFTELYAAYTPKELGYLDGEEVAPSDMEEYYKKDDIDRF
                     GVLAIYINVLNSEID"
BASE COUNT          836 a          428 c          487 g          811 t
ORIGIN      
        1 atattgatga tgaggaattt cgtaaacaat tccataattt aaaaaattta aaaagtggtt
       61 caccatcaat tttagatttt gaagaagcga ttaacgctaa atttgatgaa ggatataaag
      121 aagtaatcgt gcttccatta gcaagcggtt taagcgctac ttttaatatt gcagtaatgg
      181 cgcgtgaagg tttaagtcca gaacgcaaag ctgctacata tatacacgaa tcaaataatg
      241 cttcaattgg gatggatcta ttaatgcatt cacttgtgga acttttagac acagacccta
      301 ctgctgatga acttttagcg gcgcttgaag ctcgcgctcc acaaaaccgc gtgttatttg
      361 aaattgataa tttgcgtcat cttttccgtg gtggaaggct taatccgtta aaatacatta
      421 tcggcacagc acttaaaatc aaaccgctcg tggagtttaa agatggtgaa ttaaaagtca
      481 ttgcgcaaaa cagaagtcgg cgcaaaacga tggatgtaat cttaggaaaa attgatgact
      541 ttgtgcaaaa ttttaaaaat gtttatgttg gattatttac ttatgataaa gaagacaata
      601 tatttaaagc aatccatgat ataatcaaag aacgatggcc acatattcga attaacttta
      661 gtaaaaaaat atgtcccatc ttcacaaccc atgtgggagt tgatggttat tcagttgcgg
      721 ttgtaagtta taattaggag gaagttatgg caaaaagaaa gattgatggt ggagaattag
      781 cgctttacat tatttatggc gtgattgccc ttggtggttt aactctagtc gtattacatt
      841 taattggaat gaatcttgct aatctagaaa acgcactgcg tgttgccgat gaaacattcg
      901 cggaaaaaat gaaaatggat tttctcgtct ttggttcatt acttgttgtc ctagcgggcg
      961 cattaagtgc aattacttta gcaatctatg gtaaccgcgc ggaattagaa gaagaaaaac
     1021 gcgctcgtcg tcgtcaacgg atggcgcttg aagattttag cgatttagaa taattactaa
     1081 ttatttatga aaaaaagagc actacttaat cggtagtgct tttattttag ttgctaagta
     1141 agcggcaaat gcgaggtaat tatcactata ttttttaaaa tcaattagta atttctcact
     1201 ttgcccattt aaatgttttt gtagcgctac aacatgccac tgtaatactt ggaaaagacg
     1261 atgaaattta acacggcgca cttcatgtgg taaggctttt cttcctaaat agtaatcaag
     1321 aagattaaga ctatcactaa aattaatatt accaaaacta gcaatatcaa aatagcgatc
     1381 gttcattccg gcaaattcaa aatccactag tgtataacta tcaccgtttt taacaatgtt
     1441 tgtgcgttgg gcatccccat gcgtaaaaac gatgtcagca tctttataag tcgtagagtt
     1501 ttcattcatc caaaatgctt taagttcaaa atattctggt gttaagtgtt tttctacaag
     1561 tgactcataa cgcgcaagac gatcaatttg accgtaatca acaccttcaa tatttaaggc
     1621 atgcagttta tgaagggcac gcgctacacc agcataatct tctgctgata atgtagcggt
     1681 taagttatcg ccaggaatat attcagagat tttaatcccg gtgtctgtgt cgtaaaatag
     1741 tgttttactt gtaatattaa atggctctag taaaaaaaga tgaagtttct cagttgcgta
     1801 atcaacaaac accttccctt ccttattagc aatacggata acataatctt ttccattcac
     1861 ttttacgtga tatgtataat tactcatccc accgaggaat cgttcttgat attcgagatt
     1921 tacttcgtct ttattaagat atatcttaaa agcattaata atcttgtctt tatgcatgga
     1981 tatatcatac tactttttaa ttaaatttgt tatgattaag tatgcgcgct ggtagctcag
     2041 tggatagagc aatcgcctcc taagcggtag gtcggaggtt tgaatcctcc ccagtgcgcc
     2101 agccttttta tgaaaagaca tgaatttact ttaaaaccaa ttccatttat gcgcattaaa
     2161 aataaacaaa aagttataga aatgcgcctt tatgatgaaa agcgccaaaa gcttcgcgta
     2221 ggcgacttta tttattttac taatacagag acgaaagaaa ttttaaaagt agaaatattg
     2281 gaattaactc gctatctaaa tttcactgaa ctttacgcag cttatactcc caaagaatta
     2341 ggttatctcg atggtgaaga agttgcgcct agtgatatgg aagagtatta taaaaaggac
     2401 gatattgacc ggtttggcgt tcttgctatt tatataaatg tccttaatag tgaaattgat
     2461 tgacaactta tgcaaatagt attattataa acaagcaatt aggggccgta gctcacctgg
     2521 gagagcgcct ccatggcatg gaggaggtag cgagttcgat cc
//
LOCUS       DAOPCD010000134         2513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_32636_length_2513_cov_3.907648, whole genome shotgun sequence.
ACCESSION   DAOPCD010000134 DAOPCD010000000
VERSION     DAOPCD010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32636_length_2513_cov_3.907648"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLQ55_03810"
     CDS             <1..456
                     /locus_tag="PLQ55_03810"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPK29088.1"
                     /translation="VLVSEIIEFGVYTKLTNAVIIGAGHLGSALLNYDGFNEWGLQII
                     AAIDNNPALINTTINDKQIHGVDDLDKIIKEYNVQIAIITVPKEYAQELVDHAVAAGV
                     KAIWNFAPTHISVPKDVVLQNENMASSLSLLNYNLNLKTSKQKRKTKRK"
     gene            complement(504..2207)
                     /locus_tag="PLQ55_03815"
     CDS             complement(504..2207)
                     /locus_tag="PLQ55_03815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HPK29089.1"
                     /translation="MKFSQYPFNVPDVPKIQKQLTKYIEQFKNAKDTNAASRVMRKIS
                     KYVDDFVTDAVIISVKFSQDSRNEEYVKAQEYVDHHFPYLSALMNEYNKLLVASPFRA
                     ELEKKWGSYLFKMIETQLKTFDPSIIELLQKESQLTNEYSKLIASAQIKFDGEVYNIP
                     QMGKFTQDKNRDVRKRASAAVVKFFEENNDELGRIYHELVQVRHAMAVKLGYKNFIEL
                     GYLKLGRLDYTAKEVAGYRDQIYREVVPLAHKLYRTSLRRAGIKSPRHYDLNQSFSDG
                     NPVPLGDSKYLVKAATKMYDELSPETGEFFRFMVDKELLDLDARPGKRGGGYMTYFPR
                     YQAPFIFANFNGTAGDVDTLTHEVGHAYQGYESRHIKVSEYRQPTLEACEIHSMSMEF
                     FAWPWMESMFDDADKYRLSHLEGTIKFLPYGVAVDEFQEGVYANPEMTHEERCAMWRE
                     IEKKYLPIRNYKGAPFLEKGTFWLRQSHIFSSPFYYIDYTLAQVVAFQFATEMWKNRD
                     RAWNKYVKLCRLGGRYPFVELLAKAKLRNPFEDGNVRRVITPLKRKLKELTQVLESKN
                     D"
     gene            complement(2222..>2513)
                     /locus_tag="PLQ55_03820"
     CDS             complement(2222..>2513)
                     /locus_tag="PLQ55_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPK29090.1"
                     /translation="LETVDACVGRILEWVEKHDATIMITADHGNSEKLLDEEGKPFTA
                     HTSNPVRFGINRKDIKLMKEGGKLSNIAPTIIELLGEVPPSEMTEPSLIIKN"
BASE COUNT          761 a          481 c          453 g          818 t
ORIGIN      
        1 gttttagtat ctgaaattat tgaatttggt gtgtacacaa aattaactaa tgccgttatt
       61 attggtgctg gtcatcttgg tagtgccttg cttaattatg atggctttaa tgaatggggt
      121 ctgcaaatta ttgccgcaat tgataataat ccagcattaa ttaacactac aattaatgat
      181 aaacaaattc atggagttga tgatctagat aaaatcatta aagagtacaa cgtacaaatc
      241 gctattatca ccgtaccaaa agaatatgct caagagttag tcgatcacgc tgttgcggcg
      301 ggtgttaaag caatttggaa ctttgctcct acacatataa gtgtgccaaa agatgttgtc
      361 ttacaaaatg agaatatggc aagtagtctc agtcttttaa attacaattt aaatttaaaa
      421 acatcaaaac aaaaaaggaa aacaaaacgg aaataataag gaaaagggct gcaaattgca
      481 gcccttttaa taaaatttaa gctttaatcg tttttgcttt ctaacacttg tgttaactct
      541 tttaatttac gtttaagtgg agttataaca cgccgtacat tcccatcttc aaacggatta
      601 cgaagtttag ctttcgctag taattccaca aatggataac gaccaccaag gcggcaaagt
      661 ttcacatact tattccatgc acgatcacga ttcttccaca tttctgttgc aaattggaaa
      721 gcaacgactt gtgctaaagt gtagtcaata tagtaaaacg gactactaaa gatatgactt
      781 tgacggagcc agaatgtacc tttttctagg aaaggagcgc ccttgtaatt acgaattggt
      841 aaatatttct tttcaatttc ccgccacatg gcacaacgct cttcatgagt catctctgga
      901 ttagcgtata cgccttcttg gaattcatca acggcaacgc catagggcaa gaatttaatc
      961 gtaccttcta agtgtgacaa gcgatattta tcagcgtcat cgaacatact ttccatccat
     1021 ggccaagcaa aaaattccat gctcatgcta tgaatttcgc aagcttcgag tgttggttga
     1081 cgatattcac taactttaat atgacgactt tcgtacccct gataagcgtg tcctacttca
     1141 tgcgttaaag tgtcgacgtc accagctgtt ccgttaaaat tagcaaagat aaatggtgct
     1201 tggtaacgcg ggaagtaagt catgtaacca ccacctcttt taccagggcg agcatctaaa
     1261 tctaaaagtt ctttatcaac cataaaccga aagaattctc cggtttcagg actaagttca
     1321 tcatacattt tagttgcggc tttcactaaa tatttactgt cacctagtgg cactgggtta
     1381 ccatcgctaa agctttggtt taagtcataa tgacgtggtg acttaatacc tgcgcggcgt
     1441 aaacttgtgc gatataattt atgtgcaagt gggacgactt cacgataaat ttggtcacga
     1501 tagcctgcga cttctttagc ggtatagtca agccggccta attttaagta accaagttca
     1561 ataaagtttt tataaccaag tttaactgcc attgcatggc gaacttgcac aagttcgtga
     1621 taaatgcgac caagttcatc attgttttct tcaaagaatt taacaacagc ggcactagcg
     1681 cgttttctta catcacgatt cttatcttga gtaaatttac ccatctgcgg gatattgtat
     1741 acttcaccat caaacttaat ttgtgcactg gcaataagct ttgaatattc attcgtgagt
     1801 tgtgattctt tttgtaataa ttcaatgatt gaaggatcaa atgttttaag ttgagtttca
     1861 atcatcttga ataagtaact tccccacttc ttttcaagtt cagcgcggaa gggagaagca
     1921 acgagcagtt tattgtactc attcattaat gcacttaaat acgggaaatg atgatcgaca
     1981 tattcttggg ctttcacata ttcttcattg cgtgaatctt gcgagaattt aacactaata
     2041 ataacagcat cagtgacaaa gtcatcaaca tacttagaaa tcttgcgcat aacacgagat
     2101 gccgcatttg tgtctttagc atttttaaat tgttcaatat actttgttaa ttgcttttga
     2161 atttttggta cgtcagggac gttaaatgga tattgactaa atttcatatt tgttttcctt
     2221 tctaattttt aattattaaa cttggttcag tcatttcact aggcgggact tccccaagta
     2281 attcaatgat tgttggggca atattactaa gtttgccacc ttctttcata agtttgatat
     2341 ctttacgatt aatcccaaag cgtactggat tactcgtatg agcggtaaat ggtttacctt
     2401 cttcgtcaag taatttttcc gagttaccgt ggtcagctgt aatcataatc gttgcatcgt
     2461 gtttttccac ccattcaagg atgcgcccaa cacatgcgtc cacagtttca agt
//
LOCUS       DAOPCD010000135         2486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_33126_length_2486_cov_4.126697, whole genome shotgun sequence.
ACCESSION   DAOPCD010000135 DAOPCD010000000
VERSION     DAOPCD010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33126_length_2486_cov_4.126697"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLQ55_03825"
     CDS             <1..243
                     /locus_tag="PLQ55_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPK29091.1"
                     /translation="DGFKKKPSGEGLVHIMKTLKSAPDKTLFVGDNWRDILAGQDAGV
                     DVAFIHQHRRPHRLDVSATYVLDDISDVLGVVQSDE"
     gene            236..1159
                     /gene="trxB"
                     /locus_tag="PLQ55_03830"
     CDS             236..1159
                     /gene="trxB"
                     /locus_tag="PLQ55_03830"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPK29092.1"
                     /translation="MNKEIIPILIIGAGPAGMTAAIYLSRANATFKIVDKYAPGGKMN
                     ITQDVENYPGIETISGPDLAFKMFEQLRSLGIEVGYADVNKITKEDDYFVIHSDEETL
                     YAKAVIVATGTQERKLKIPGEARYTGKGVSYCAICDGNLYKNDAIMIIGAGNSAIEEA
                     LFVSKIVKHVHIVHRNERFRADESLVNELKAMPNVTFHLNYIPVEILGDTKGVTSVAI
                     KNVIDGTDKVIEVKAVFPFVGSDASADFLSEFDVRDDKGFVLVNEEMMTSVPGLFSIG
                     DVNQKVLRQIVTATNDGAIAALAANRFVKRK"
     gene            1219..2196
                     /gene="whiA"
                     /locus_tag="PLQ55_03835"
     CDS             1219..2196
                     /gene="whiA"
                     /locus_tag="PLQ55_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HPK29093.1"
                     /translation="MKNVSFTTRAKEDITFNTISYSLPRRKATLAALIKHAGHLIIRE
                     KKEVLKLSTENAKVAKYIYQLFKDIFNFEPTFSYVTKRKFGKKMKFNVIVEQKVDEII
                     ETLAVDFYDSAVSRKIVYSDDTLGGYFTGAFLGSGSMTSPLSSNYHLEIVVRQKKYAE
                     HLARMLNRFEKTNFNAKVTARRDKYIVYLKRSDQISSFLVFVGAANASLEFEGIRMDR
                     DMQNITNRMFICDEANYNKTVKSATEQIENIKIVQRVYGHTPHNNEKLGALMRLRLEN
                     EDATMNGLAEMLTEELGSEKRITKSNVNHLFIKLRELAEPFKELSRGNK"
     gene            2183..2404
                     /locus_tag="PLQ55_03840"
     CDS             2183..2404
                     /locus_tag="PLQ55_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPK29094.1"
                     /translation="MEINEAFGIRLRQLRKAKGWSLLELSVRANVNHNYLCDLENGRR
                     NPTLALLYRISEAFGITLSSLLLGIELIQ"
BASE COUNT          831 a          384 c          540 g          731 t
ORIGIN      
        1 gacggtttta aaaagaaacc aagtggtgaa ggcttagtgc acattatgaa aacgctaaaa
       61 agtgcacctg ataaaacact ttttgttggt gataattggc gtgatatctt agccggtcaa
      121 gacgcaggag tcgatgttgc gtttatccat caacatcgcc gtccgcatcg ccttgatgta
      181 agtgcaacat atgtattaga tgacattagt gatgtcttag gagtagtgca aagtgatgaa
      241 taaagaaatt attccgattt taattattgg tgcgggtccg gcggggatga cggcggcaat
      301 ttatctttcg cgcgcaaatg cgacatttaa gattgttgat aaatatgcgc caggcggcaa
      361 aatgaatatt acgcaggacg tcgaaaatta tccaggtatt gaaaccatca gcggacctga
      421 ccttgccttt aaaatgtttg aacaactacg cagcttaggg attgaagtgg gttacgctga
      481 tgtaaataaa attaccaaag aagacgatta ttttgtaatt catagtgatg aagagacgct
      541 ttacgcaaaa gctgtcattg tcgcaacggg tacccaagag cgaaaactaa aaataccagg
      601 tgaagcgcgt tacaccggta aaggtgtaag ttactgcgct atttgtgacg ggaacttata
      661 taaaaatgat gcgattatga ttattggcgc tggtaatagt gcaattgaag aagcactctt
      721 tgtaagtaaa atcgttaaac atgtgcatat tgttcatcgt aatgaacgct ttagagcaga
      781 tgaatccctt gttaatgaac ttaaagcaat gccaaacgtt actttccact taaactatat
      841 tccagttgaa atcttagggg acactaaagg tgtcactagt gttgcaatta agaatgtaat
      901 tgatggcacc gacaaagtta ttgaagtcaa agccgtgttc ccgtttgtgg gaagtgatgc
      961 atccgcggat ttcttaagtg aatttgatgt ccgtgacgat aaagggtttg tgttagtgaa
     1021 tgaagaaatg atgacgagtg tccctgggct ctttagtatt ggtgacgtga atcaaaaagt
     1081 attacggcaa attgtaaccg ctacaaacga cggtgcgatt gctgctctcg cggcgaatcg
     1141 ttttgtgaaa cgaaaataac taattagtaa ataagtgaaa tttgggggaa attccccctt
     1201 ttcctttata atataaatat gaaaaatgtg tcatttacta caagagcaaa agaagatatt
     1261 acttttaata cgatatctta ttccttacca agacggaaag caacacttgc cgcgctcatt
     1321 aagcatgcag gtcatttaat aattcgtgag aagaaagaag ttttaaaact tagcacggaa
     1381 aacgctaagg tagcgaaata tatttatcaa ttatttaaag atatctttaa ttttgaacca
     1441 acattttcgt atgttacaaa acgtaaattt gggaaaaaga tgaaatttaa tgtgattgtt
     1501 gaacaaaagg ttgatgaaat tattgaaaca cttgcggttg atttttatga tagcgctgtg
     1561 agtcggaaga ttgtatatag tgatgatact ttaggtggat actttacggg tgctttttta
     1621 ggcagtggtt caatgacaag tcccttaagt tccaattacc atttagaaat tgttgtgcgc
     1681 caaaagaaat acgccgaaca cttagcgcga atgcttaacc gttttgaaaa gacaaacttt
     1741 aacgctaaag taactgcaag acgtgataaa tatattgtgt acttaaagcg aagtgaccaa
     1801 atttcgtcgt ttcttgtctt tgtcggagcg gcgaacgcta gtttagaatt tgaagggatc
     1861 agaatggacc gcgacatgca aaatattacg aaccggatgt ttatatgcga cgaagctaat
     1921 tacaataaaa cagttaaaag tgccactgaa caaattgaaa acattaagat tgtacaaaga
     1981 gtgtacggac atactccgca taataatgaa aaactcggag cactgatgcg tttacgttta
     2041 gaaaatgaag acgcgacaat gaatggactc gcggaaatgt taacagaaga attaggaagt
     2101 gaaaaaagaa ttactaaaag taatgttaat catttattta ttaagttacg ggaactagca
     2161 gaacccttta aggagttaag tcgtggaaat aaatgaagcc tttgggatta gattacggca
     2221 attacgaaaa gcaaagggct ggagtctttt agaactttca gtacgggcga atgtgaatca
     2281 caattattta tgtgatttag aaaacggaag acgtaacccc actttagcgc ttctctaccg
     2341 cattagtgaa gcctttggta ttactttatc atccttactt ttagggattg aactcattca
     2401 ataaaactaa ttttgattaa aaaaagtgga attcgggata tttttaccta ataagtgaaa
     2461 atattcgttt ttttgtatgt ttttgt
//
LOCUS       DAOPCD010000136         2477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_33299_length_2477_cov_3.196945, whole genome shotgun sequence.
ACCESSION   DAOPCD010000136 DAOPCD010000000
VERSION     DAOPCD010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33299_length_2477_cov_3.196945"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="PLQ55_03845"
     CDS             <1..1241
                     /locus_tag="PLQ55_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK29095.1"
                     /translation="FTVQIGVYVPATNSPDGTRAQLYLNKLAVTTTAEENPFELHNYT
                     DANELLLRRSDKEKIPYRYQTVISGNQMLYEGRVIRASFEYDYYKAVYGEVNRIIGIS
                     DLLNARRDGKVKFDLDPAHSDHTKPDFNYQIVMDTFEVVNDKTSQYREITFAHPRQTV
                     KAGMTTIYVYNYDVVVSRYREMGYTREPFFLFGTNQRGQDFFKIVFNGLRTSLILGFS
                     AAAINIFIGLVWGAISGYYGGTVDILMERFTEILGGVPWIIIMTLTILHLGNNFGVFL
                     LALILTGWIGTASVTRSQFYRYKRREYVLASRTLGAKDSRLIFKHILPNSIGPIVTGA
                     VMMIPGIIFSEATISYLGLGLQGLTSLGVALSEVQAYIYTDPYLIVTGSLIIALLMIS
                     FNLFGNGLRDAFNPSLKGVE"
     gene            1241..>2477
                     /locus_tag="PLQ55_03850"
     CDS             1241..>2477
                     /locus_tag="PLQ55_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK29096.1"
                     /translation="MKHTDKEVVIKVEDLSISFSTDYGRLHAVRGVSFELYRGEVLAI
                     VGESGSGKSVSAKTIVGILAVNGRVEGGRIMYGGEDLTKVSEEEFHRIRGKKIGMIFQ
                     DPLSSLNPLMRIGKQITEAIIINGNKLKLMYEDLIYEENVAYRNAVTKFRDEIDTSAF
                     KLRNLKVGNKFKAQDIRADKALTKEEKRAKLDALIEEEKAADLEHRALVRRVKAESKP
                     VLTELKAKLKVAQKKAKAEVKAYHKEIKAEYKKERDTLRAELRATKNEERRKELKEAL
                     IAAKRNYIDKTRVTKQVAHDMAVKVMEEVGIPNAARRFRQYPFEFSGGMRQRIVIAIA
                     LIQNPDILICDEPTTALDVTIQAQILELILRLKRERQLSVIFITHDLGVVANMADRVA
                     VMYAGRIVEYGKVEEIFYNP"
BASE COUNT          774 a          454 c          525 g          724 t
ORIGIN      
        1 aattcactgt acaaattggt gtttatgtgc ccgcaactaa tagtccagat ggtacgcgcg
       61 cgcaattata tttaaataaa ttagcggtaa cgactactgc tgaagaaaat ccttttgaac
      121 tacataacta cactgatgcg aatgaattat tactacgccg aagtgataaa gaaaaaatac
      181 catatcgcta tcaaacagtt atttctggta atcaaatgct ctatgaaggt cgtgtcattc
      241 gcgctagttt tgaatacgat tactataaag cagtttatgg tgaagttaat cgtatcattg
      301 ggattagtga tttactaaat gcccgtcgtg atggcaaagt taaatttgac cttgatccgg
      361 cccatagcga tcatacaaaa ccagatttta actatcaaat cgtgatggat acatttgaag
      421 ttgtaaacga caaaacatca caatatcgcg aaattacttt tgcccatcca cggcaaacag
      481 ttaaagctgg gatgacaaca atttatgtat ataactatga tgtcgttgtt tctcgttatc
      541 gggaaatggg ttacacacgt gaaccattct tcctttttgg cacgaatcaa cgtggtcaag
      601 atttctttaa gattgtcttt aacggtttac gcacatcctt aattttaggc tttagtgctg
      661 ccgctattaa tatttttatc ggtttagtct ggggtgccat cagtggttac tacggcggaa
      721 cagttgatat tttaatggaa cgctttacgg aaatcttagg tggggtaccg tggatcatta
      781 ttatgacctt aacaatctta cacttaggga ataactttgg cgtcttcctt ttagcgctta
      841 ttctcacagg ctggatcggc acggcaagtg taacgcgtag tcaattctat cgttataaac
      901 gccgagaata tgttttagca agtaggacac ttggggcgaa agatagtcgc ttaattttca
      961 agcacatctt acctaactcc attggtccaa ttgtgaccgg tgccgtcatg atgattccag
     1021 gtatcatctt tagtgaagcg acgattagtt acttagggct tgggttacaa ggcttaacaa
     1081 gtcttggggt cgcgctcagt gaagtccaag catacattta tactgatcca tacttaattg
     1141 ttaccggttc acttattatc gctttactca tgatttcctt taacctcttt ggtaacggac
     1201 ttcgtgatgc atttaaccca tcattgaaag gagtcgaata atgaaacata cagataaaga
     1261 agtagtcatc aaagttgaag acttatcgat ttcctttagt actgactatg gccggttgca
     1321 cgctgttcgt ggggtaagtt ttgaactcta tcgtggtgaa gttctcgcca tcgttggtga
     1381 aagtggctca ggtaagagcg tatctgctaa aacgatcgtg ggcattttag cggttaatgg
     1441 tcgtgttgaa ggtggcagaa taatgtatgg tggtgaagac ttaacgaaag tcagtgaaga
     1501 agaattccac cgtattcgtg ggaaaaagat tggaatgatt ttccaagacc cactctcaag
     1561 cttaaatcct ttgatgcgta ttggtaaaca aattactgaa gcgattatta ttaatggtaa
     1621 taagcttaaa ttaatgtatg aagacttgat ttatgaagaa aatgttgctt atcgtaacgc
     1681 tgtcacaaaa ttccgtgatg aaatcgatac tagtgccttt aaacttcgta acttaaaagt
     1741 cggtaataaa tttaaagcac aagatattcg cgctgacaaa gcattaacga aagaagaaaa
     1801 acgcgctaag ttagacgctt taattgaaga agaaaaagct gctgatttag aacatcgtgc
     1861 gcttgttaga cgcgtgaaag cagaaagtaa accagtttta accgaactta aagctaaact
     1921 caaagttgcg caaaagaaag ctaaagcaga agtaaaagct tatcacaaag aaattaaagc
     1981 tgagtataaa aaagaacgcg atacattacg cgctgaactc cgcgctacga aaaacgaaga
     2041 gcgtcgtaaa gaattaaaag aggcgttaat tgctgcaaaa cgtaactata tcgataaaac
     2101 aagagtgaca aaacaagtcg cacacgatat ggccgttaaa gttatggaag aggtcggtat
     2161 tcctaatgct gcccgacgct tccgtcagta cccatttgaa tttagtggtg ggatgcggca
     2221 acggattgtt attgcgattg ccttaattca aaatcctgac atcttaattt gtgacgagcc
     2281 cacaacagcg cttgacgtta caattcaagc gcaaatctta gaacttattt taagacttaa
     2341 acgcgaacgg caattatccg ttatctttat tacgcacgac ctcggtgttg tcgccaatat
     2401 ggcggaccgc gtcgccgtga tgtatgcggg tcgaattgtc gaatatggta aagttgaaga
     2461 aatcttctac aatccaa
//
LOCUS       DAOPCD010000137         2429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_34141_length_2429_cov_3.474726, whole genome shotgun sequence.
ACCESSION   DAOPCD010000137 DAOPCD010000000
VERSION     DAOPCD010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34141_length_2429_cov_3.474726"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="PLQ55_03855"
     CDS             complement(<1..974)
                     /locus_tag="PLQ55_03855"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPK29097.1"
                     /translation="MKNLMILNDAALKKTLAKMHPYDIATKMKDASGDTQMRLIRLMA
                     LNKTVEVFLELPSEVSRHYFSLLTETQKKQFLEAFEMDDLKTFITSFEDDYQMYLLSL
                     LGTHKAIALKVLMSYDDEKIATIMSTNFVTITQNSTIKDTTAHIINSSKYNDFVDDIF
                     VINENNALIGSITLKDLISARATDTLDSITYSINNYLTLDDSLNDGYKKFENYAKSVL
                     PVLDDDMKIVGIVTADDVLNIMVAEHEEDIAKMTGVGDYDESSNAFRRSIQRMPWLVI
                     SVLLNLVIAALLSVFEKTLVEVIALIMFQPMILGMAGNIGTQSIAVTI"
     gene            complement(975..2279)
                     /locus_tag="PLQ55_03860"
     CDS             complement(975..2279)
                     /locus_tag="PLQ55_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPK29098.1"
                     /translation="MRTIIKRKDKKALLKYVKKMHPYDIIEALNTLTLDEQELFYSLL
                     SEEEIGRIVAFMEPEKAAEVIDDFDLTKQKNIIDTLELDDAADIIVYLDNETKLIEGL
                     EKELHITQVLKYDEEKVGAHMSDAYISLSLGTDIKAATKNVIQKAGEIDVINHIFIVD
                     SGNKYKGTVELRDLVRTKAPALIDDITEFTPFVFASDDIEESVHKMKNYGLHEMAVVS
                     EELELIGIITIDDAIEIYHEESVDDFEKLSALPDTKTKGVIKSAAKRLPWLFLLLVLS
                     MPIAIATSRFEEVITSVAVLALFQPLILDAGGDVASQTLAVTLRLLSKNPKKALRNGG
                     TEILTGIINGLGLGIVAGIVSFIISKVIGITEPFNVSLVVFISLAVTVATGPIFGLFI
                     PLILDKMKIDPAVASSPLITTLIDITSVVIYFGVATLLLGVI"
BASE COUNT          760 a          452 c          388 g          829 t
ORIGIN      
        1 aaaatcgtca cggcaattga ttgagtgcca atgtttcctg ccatccctaa aatcattggt
       61 tgaaacataa ttaaggcgat gacttcaacg agtgtctttt caaatactga taatagggca
      121 gcaatcacta agtttaataa tactgaaata actaaccacg gcattctttg tatagaacgt
      181 ctaaaggcat tggatgattc atcataatca ccaacccctg tcatcttagc gatgtcttct
      241 tcatgttcag cgaccataat atttaataca tcatcagcag ttacaatccc gacaattttc
      301 atatcgtcat ctagtactgg taagacactt tttgcatagt tttcaaactt tttataacca
      361 tcattaagtg agtcatcaag tgttaaataa ttgttaatgc tataagttat gctatcaagt
      421 gtatcagtgg cgcgtgcact aattaaatct tttaaagtta tacttccaat taaagcatta
      481 ttttcattaa ttacaaagat atcatcgaca aaatcattgt attttgagct attaatgata
      541 tgcgcagtag tatctttgat cgttgaattt tgtgtaatcg ttacaaaatt agtgctcatt
      601 attgttgcaa ttttttcgtc gtcataactc attaaaactt taagtgctat agctttatgt
      661 gtccctagta aacttaatag atacatttga tagtcgtctt caaaactagt aatgaatgtt
      721 tttaaatcat ccatttcaaa agcttctaag aattgctttt tttgtgtttc cgttaataaa
      781 ctaaagtagt gacgcgaaac ttcacttggt aattcaagga acacttcaac tgttttattt
      841 agtgccatca aacgaattaa tcgcatttgc gtatcgccgg aagcatcctt catttttgtc
      901 gcgatgtcat aaggatgcat tttcgctaaa gttttcttta aggcggcatc atttaaaatc
      961 attaagtttt tcatttaaat cacccccagt aataatgttg ctaccccgaa ataaataaca
     1021 acactagtaa tatcaataag tgtagtaata agtgggctag aagcgactgc tggatcaatt
     1081 ttcatcttgt ctaaaataag cgggatgaat aatccaaata ttggtccagt tgcgaccgtc
     1141 accgctagtg aaataaagac gactaatgat acattaaagg gttcagtaat cccaattact
     1201 ttggaaataa taaaagagac tatacccgcg actataccaa gcccaagccc gttaataatt
     1261 cctgttagta tttcggtgcc accatttcta agcgcttttt ttggattttt actaagtaat
     1321 cttaaagtta ccgcaagggt ttgactcgca acatcacccc cagcgtcaag aattaatggt
     1381 tggaaaaggg cgagtaccgc tactgaagta attacttctt caaagcgact tgtagcaatt
     1441 gcaattggca ttgatagcac tagtagtaag aagagccacg gtaatctttt agcagcggac
     1501 ttaataactc cttttgtttt tgtatctggt agtgccgaca gcttttcaaa atcatcaacg
     1561 gattcttcat gatatatttc gatcgcatca tcaatcgtaa taataccgat aagctctaat
     1621 tcttcgctga caactgccat ttcatggaga ccataattct tcattttatg gactgattct
     1681 tcaatgtcat cactggcgaa aacaaacgga gtaaattcag tgatatcatc aataagtgct
     1741 ggtgctttag tgcggactaa atctcttaat tcaaccgtcc ctttatattt attgccacta
     1801 tcaacaataa agatgtgatt aataacatca atttccccag ctttttgaat aacattttta
     1861 gttgccgctt taatatctgt tcctaaagaa aggctaatat atgcatcaga catatgggcg
     1921 ccaacttttt cttcgtcata ctttaatact tgcgtaatat gaagttcttt ttctaaacct
     1981 tcaattaact tagtttcatt atcgagatag acaataatgt cagcagcgtc atcaagttca
     2041 agcgtatcaa taatattttt ttgttttgtt aaatcaaaat catcaataac ttctgcagct
     2101 ttttctggtt ccataaaagc aacaattctg ccaatttctt cttcgcttag cagtgagtaa
     2161 aatagctctt gttcatcaag agtaagtgta tttagtgctt caataatgtc ataaggatgc
     2221 atttttttga catatttaag caatgctttt ttgtcttttc gtttaataat tgtgcgcatg
     2281 ttaacctccc tcctttgctt ggaattcaaa taaaaagctc ccacattaag tgagagctaa
     2341 ctaaacagta cgagtatcag cgtattcgta gagttttagc actatgcaac gtaaacatga
     2401 agttagctac tttgtggaaa gccttaagt
//
LOCUS       DAOPCD010000138         2407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_34554_length_2407_cov_2.610544, whole genome shotgun sequence.
ACCESSION   DAOPCD010000138 DAOPCD010000000
VERSION     DAOPCD010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34554_length_2407_cov_2.610544"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1303
                     /locus_tag="PLQ55_03865"
     CDS             65..1303
                     /locus_tag="PLQ55_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013410846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK29099.1"
                     /translation="MQEIKRDIYLNKLINAIGNRMIKVVTGIRRSGKSYLLNNLFKNY
                     LLESGVSADHIIILNMDERRNISYRDPDTLDEYIRSKIKDNEMYYVIIDEVQFVADFE
                     SVLNGFLNMHNLDVYVSGSNSKFLSNDIITEFRGRGQQIRVYPLSFSEFMSVTTLNKS
                     DALTEYLRYGGMPLLMSMKSDEAKARYLKDLFELTYLKDIIERNNVLRDDVLDELVNI
                     LASAVGSLTSVTKLTNTFKSKGVKELSKNTLIQYLDYLLDSFLIEKVSRYDVKGKKYI
                     QTLAKYYFSDIGLRNARLNFRQQEENHIIENVVYNELIRRGFNVDVGIVEIRNGNGIK
                     RLEVDFVCNEASRRYYIQVTTHLDTREKTLQEQRPLLHIGDNFKKIIIVRDDIKPWYT
                     EEGILVLGLYDFLLDNDSIL"
     gene            1477..1740
                     /locus_tag="PLQ55_03870"
     CDS             1477..1740
                     /locus_tag="PLQ55_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29100.1"
                     /translation="MQTLNYIGLIVVALLLIPNLMYYLKHKEEVVSKNGIFKTLAIIG
                     ISGSLFFMVINIPYTWLQFYFMFAREMYFISNVNSLLFMYLFS"
     gene            1815..2407
                     /locus_tag="PLQ55_03875"
                     /pseudo
     CDS             1815..2407
                     /locus_tag="PLQ55_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
BASE COUNT          822 a          298 c          450 g          837 t
ORIGIN      
        1 caaatatttt aaaatagtac tgaacaaaaa ctacaaaagc gtaagttttg ttcagtgagg
       61 tgttatgcaa gagataaaac gtgatatata tttgaacaag ttaattaatg caattggcaa
      121 ccgcatgatt aaagtagtga ctggtattcg gcgctcaggc aaatcatatt tgttaaataa
      181 tcttttcaaa aattatttat tagaaagtgg tgttagtgca gaccacatca ttattttaaa
      241 tatggatgaa aggcgaaata tttcttatcg tgaccccgat actttagacg aatacattcg
      301 cagcaaaatt aaagataatg aaatgtatta tgtcattatt gatgaagtgc agtttgttgc
      361 cgattttgaa tcagtgctta atggatttct taatatgcat aacttagatg tttatgtttc
      421 tggtagtaat tcaaaatttc tatcaaatga tataatcact gaatttaggg gtagaggaca
      481 acaaattcgc gtgtatccac tttcttttag tgaatttatg agtgtcacca ctttaaataa
      541 aagtgatgct ttaactgaat atttacgcta tggcgggatg ccgcttttaa tgtcaatgaa
      601 aagtgatgaa gcaaaggcgc gttatcttaa agatttattt gagcttactt atctcaaaga
      661 cattatcgag cggaataatg tgctgcgtga tgatgttttg gatgagcttg taaatatatt
      721 ggcctctgct gttggttctt taactagtgt tactaaatta acgaatacct ttaagagtaa
      781 aggtgtcaag gaactatcga aaaatacgct tattcagtat ttagattacc tattagattc
      841 ttttcttatc gagaaagtta gtcgttatga tgttaaaggt aaaaaatata ttcaaacttt
      901 agcaaagtat tattttagtg atattggatt acgtaatgcc aggcttaatt tccggcaaca
      961 agaagaaaat cacattattg aaaatgttgt ctataacgaa cttataagac gtggttttaa
     1021 tgttgatgtt ggtatcgttg aaattagaaa tggaaatggt attaagcgcc ttgaagttga
     1081 ttttgtttgt aacgaggcga gtagacgcta ttacattcaa gtaacgacgc atcttgatac
     1141 tagagaaaaa acattgcaag aacaaagacc actgctacat attggcgata actttaagaa
     1201 gattattatt gtccgcgatg atattaagcc ttggtataca gaagaaggta ttttagtgct
     1261 aggactttat gatttcttac ttgataatga tagtattctc taaacactta ttgtaggctg
     1321 ttctttaatt ggattgtgct taatataata aaaaatgaaa ggttttttaa ttgtcatttc
     1381 ttacatattc tttacaaaca agcagcaata aaagtattga acttatgtta aaattatgtg
     1441 gaataaagac aaagggtaat gttaggagta atagttatgc aaacattaaa ttacattggt
     1501 ttaattgtgg tagcgttact tttaatacct aatttgatgt actatctaaa acacaaagaa
     1561 gaagttgttt ctaaaaatgg tatctttaaa actttagcga taattggtat cagtggatca
     1621 ctatttttta tggtgattaa tattccttat acttggctac aattttattt tatgtttgcg
     1681 cgagaaatgt actttattag taacgtaaac tcgctattat ttatgtattt attttcttag
     1741 taacttggga taaaaagagt gtagtacgcg cgatattatt aacgattatt cctttttgta
     1801 tatttacttt ttcagcgata atgcttggtt gtttagatga aaaaattacc gctgttaacc
     1861 cggattcaaa attatcttta tttggtcaag aatataatcc tgaaacttat gcaatagcaa
     1921 aagctgacat gttaattaaa ggtggtaatg cccaaaacat gaaatttggc aatactttaa
     1981 atgacgacca atttagtggt tatgaatttg attatattat ttcaaatccg ccatttggga
     2041 ttgattggaa aagtgaagca aaggatgtta agaaagaaca tgaaaaatta ggtttcagtg
     2101 gtagatttgg ccccggttta ccagcaataa gtgacagtca aatgttattt atgttaaatg
     2161 gtgtcaaaaa gttaaaagaa ggaagcggaa gaatggctat cattcaaaac ggctcttcac
     2221 tttttactgg tgatgctggt agtgggccaa gcgaaattag aagatatatt attgaaaacg
     2281 atatgttaga agcgattatt cagttaccaa cagatttatt ttataatact tcgattacaa
     2341 cctatatatg gttaattaca aaaggaaaat caagtgaacg tattggtaaa gttcacttga
     2401 ttgttgg
//
LOCUS       DAOPCD010000139         2405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_34600_length_2405_cov_3.231064, whole genome shotgun sequence.
ACCESSION   DAOPCD010000139 DAOPCD010000000
VERSION     DAOPCD010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34600_length_2405_cov_3.231064"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="PLQ55_03880"
     CDS             complement(<1..1026)
                     /locus_tag="PLQ55_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPK29101.1"
                     /translation="MRKFNLSVHELVDFVLHSGDLDTRIFNLETMTRGTQIHAFYQAK
                     QNDKYLAEYALEYTFEYENHLITLQGRVDGVIVNGSEVTIEEIKSANTDIDKFFENNE
                     AWHLGQAKCYALMYALRFNLDVIDIQLTYISQIDERKMMKRYTFTREELQSDVFSYFG
                     EYLAFFYQVEKYEKRRTKRLKALNFPYKEVRSSQKVMMEQVEHALEHEHKIFLNAPTG
                     SGKTMAVLYPSLKVLAEKTNTKIFYLTAKGSGKAQAENALRVVREKALVKSTTVSAKE
                     KMCLNDKVACNPDECPYAVNYYGKIRNALLDAFKSRDEYNTEIVTEYAKKHELCPFEF
                     QLDLTLLS"
     gene            complement(1013..1966)
                     /gene="fmt"
                     /locus_tag="PLQ55_03885"
     CDS             complement(1013..1966)
                     /gene="fmt"
                     /locus_tag="PLQ55_03885"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPK29102.1"
                     /translation="MKDLRIIYLGTPDFSAELLRYLIEAGYNIIAVVTQGDSPVGRKR
                     VLTPSPVKTLALAHNIPVFTPLRIRKEYDFIVELEPDLILTFAYGQIVPKAVLEAPKY
                     GCLNFHGSILPKYRGASPIQMALINAEEETGVSLMEMVEAMDAGRVFGIEKFKIEPSD
                     NFLTIASKMVKASATLIEEVLPSVISGENKGVQQEESQVTFAPLLKIKDEWINLENDD
                     VKNVLGRIQAFAPATGALLSYDDLPLKIYEARFDNNNVTRTIGELFISEKKLLLQVKG
                     GQIEVLKLQKSGKKIIDARSFINGEKHKLPTILKGYIDAEI"
     gene            2105..2380
                     /locus_tag="PLQ55_03890"
     CDS             2105..2380
                     /locus_tag="PLQ55_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29103.1"
                     /translation="MPKRIKKIIFALYSMVLWTTILVIYIFFKDPVILIYVLGAIMIT
                     LSLLILFLKDKELHVPCIHITMLIHTALFYLCFCSANTRMECLRYEG"
BASE COUNT          725 a          462 c          369 g          849 t
ORIGIN      
        1 actcaaaagt gttaaatcta actgaaattc aaacggacat aattcatgtt ttttggcata
       61 ttcagtaaca atctctgtgt tatattcgtc cctactctta aaagcatcaa gtagcgcgtt
      121 tctaatcttc ccgtaataat taacggcata tggacattca tcaggattac aggcaacttt
      181 atcattaaga cacatctttt ctttggcgct gactgtagta cttttaacta gtgctttttc
      241 gcgcactact cgtaaagcat tttcggcttg tgccttccca cttccttttg cggttaagta
      301 aaagatttta gtattagttt tttccgctaa aactttaaga ctaggataaa gtaccgccat
      361 agttttaccg ctaccagttg gtgcatttaa gaatatttta tgctcatgtt ctaaggcgtg
      421 ctctacttgt tccatcatta ctttttgact agaacgtact tccttataag gaaaattaag
      481 tgctttaagt ctctttgtac ggcgcttttc atatttttcg acttgataga aaaacgctaa
      541 gtattcacca aaataactaa aaacatcact ttgtaattct tcacgcgtaa atgtatagcg
      601 tttcatcatc ttgcgttcat caatttgtga aatatatgtt aattgaatat caataacgtc
      661 taagttaaaa cggagcgcat acatcaatgc ataacacttt gcttgtccaa gatgccatgc
      721 ttcattattt tcaaaaaatt tatcaatatc agtgttcgca cttttaattt cttcaatcgt
      781 tacctcactt ccattaacaa taaccccgtc tactcttcct tgtaatgtaa taagatgatt
      841 ttcatattca aaagtatatt caagtgcata ttcggcaaga tatttatcat tttgtttagc
      901 ttgatagaag gcgtgaatct gtgtacctcg cgtcattgtt tctaaattaa aaatgcgggt
      961 atctaaatcc ccagaatgta aaacaaagtc cacgagctcg tgaacactga ggttaaattt
     1021 ccgcatcaat atatcctttt aaaatggtcg gtaatttatg cttttcacca ttaataaaac
     1081 tacgagcatc aattattttc ttgccgcttt tttgcagttt aagtacttca atttgtccgc
     1141 cctttacttg taataaaagt tttttctcac taatgaaaag ttcaccgata gtgcgagtaa
     1201 cattattatt gtcaaagcgt gcttcgtaaa tttttaatgg taagtcatcg taacttaata
     1261 acgcacctgt agcgggcgca aaagcttgta tccgccctaa aacattctta acatcgtcat
     1321 tttcaagatt aatccactca tctttaatct ttaaaagcgg ggcaaaagta acttgtgatt
     1381 cctcttgctg cacaccttta ttttcgccgc taataacact tggtaaaact tcttcaatca
     1441 gtgtcgcact tgcttttacc atcttacttg caattgttag gaagttatcg cttggttcaa
     1501 ttttaaattt ttcaatcccg aacactcggc cagcgtccat tgcttcaacc atttccataa
     1561 gactaacacc cgtttcttct tcggcattta ttagtgccat ttgaattgga gaagccccgc
     1621 gatattttgg caaaattgag ccgtgaaaat ttaaacaacc atatttaggt gcttctagta
     1681 ctgctttagg tacaatttga ccataggcaa aagtaaggat taagtccggt tctagttcca
     1741 ctatgaaatc atattcttta cgaatgcgaa gcggagtaaa aacgggaata ttatgcgcta
     1801 atgctaaagt ctttacggga cttggtgtta atactctttt gcgcccaaca ggactatcac
     1861 cttgtgtgac aacggcgata atgttataac ctgcttcaat taaatagcgt aaaagttcag
     1921 cgctgaagtc tggtgttcct aaataaataa ttcttaaatc tttcatactc ttcacgctta
     1981 aattatagac aaaaattcac acttcgtaat gaaatatatt aaatcctcgt cccataatat
     2041 tgaatttact agctttaatg gctataattt aaaacatatg ttgaactcta ggaaaaataa
     2101 acatatgcct aaaagaatta aaaaaattat ctttgcctta tattcaatgg tgttatggac
     2161 gacaattctc gttatttata tattttttaa agatccagtt atcttaatat atgtgctagg
     2221 ggcaataatg attacattgt cactacttat tttattttta aaagataagg aattacacgt
     2281 tccttgtatt cacattacaa tgcttatcca tacggcgctt ttttatctat gcttctgttc
     2341 cgctaatact cggatggaat gcttacgata tgaaggctag tgtcgtcata cttataatat
     2401 ttggg
//
LOCUS       DAOPCD010000140         2385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_35018_length_2385_cov_4.012017, whole genome shotgun sequence.
ACCESSION   DAOPCD010000140 DAOPCD010000000
VERSION     DAOPCD010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35018_length_2385_cov_4.012017"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /gene="proS"
                     /locus_tag="PLQ55_03895"
     CDS             complement(<1..1324)
                     /gene="proS"
                     /locus_tag="PLQ55_03895"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPK29104.1"
                     /translation="MSKIKIDAITTQSEDFAQWYTDVCRKAELMDYSEVKGFIIYRPY
                     GYAIWENIQKYLDEKFKEKGHDNVYMPLVIPTSLFQKEKDHIEGFAPETLVATQGGNQ
                     KIEDPLIIRPTSEVLFCDHYAKIVSSYRDLPKKYNQWCSVVRWEKTTRPFLRGSEFLW
                     QEGHTIHETEVEARTHALDMLKVYENMGKELLAIPFLTGTKTEKEKFAGAEQTFTIEA
                     LMPDGKALQCGTTHYFGDGFARVFNIQFQDRNNTLQYVHQTSFGVSTRLIGSVIMAHG
                     DDNGLSLPPRLAPVQVIIVPIQQNKPGIMPKALELVSRLKAIGVRVKIDDSDKSPGWK
                     FSEHEMKGIPLRLEIGPRDLKAEVLSGSIRHSHEKISFADKDLEEVVPALLEKIHEDM
                     YQKKLTDLLNHTIEVRSIEELEDALNNKLAYAKMMWSGDQEDLDVIKEK"
     gene            complement(1327..>2385)
                     /locus_tag="PLQ55_03900"
     CDS             complement(1327..>2385)
                     /locus_tag="PLQ55_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29105.1"
                     /translation="RVIRFVDNHTVGIYFPRSSDVDTAIDVFQSIKKRMSGYLQKNGY
                     NEYSLGIGLTRSRQFSEMNMFFKTAEELSLYALKEDGRLLIHDPNKEIKDMDLSLYRS
                     ELTQLVARKGVDTLFQPIVNAKTVEILGYLTTFSISGSLFGSFNEAAQFARENKQNEQ
                     LVKMVLRKAAARYYNMRRNNKQLLFVPLGLGGVDFISDAFSTQPFANDLKMVFMLEEQ
                     DFANTFVNIEDMLKLIKKIKKQKYEVCLIISDTELTLPDKIYGEVDYFMVNEKILTHT
                     VNDERERLSLLSSLGKLLRHQKPIMISDLPKWSEIEYYIRAGVDYVASDEISKKDNSL
                     QTIDKKKALRIINFSKRK"
BASE COUNT          697 a          491 c          388 g          809 t
ORIGIN      
        1 atttttcttt aatcacatct aagtcttctt gatcaccact ccacatcatc ttagcgtacg
       61 ctaatttgtt gttaagggca tcttcaagtt cttcaatact tcttacttca atggtgtgat
      121 taagtaaatc agtaagtttc ttttgataca tatcttcatg aatcttttct aagagtgccg
      181 gcactacttc ttctaaatct ttatcggcaa aactaatttt ttcatggcta tgacgaattg
      241 aaccacttaa aacttccgcc tttaggtcgc gcggaccaat ttctaagcgc agcgggatac
      301 ctttcatttc atgttcacta aatttccagc ctggtgactt atcggagtca tcaattttaa
      361 ctctaacacc aattgctttt aagcgactga caagttctaa ggcttttggc ataattcctg
      421 gtttattttg ttggataggg acaatgataa cttgaacagg agcaagccgt ggtggtaggc
      481 taagcccatt atcatcgcca tgcgccataa taacactgcc gattaaacgc gttgaaacac
      541 caaaactagt ttgatgcaca tattgtaaag tgttattacg atcttggaat tgaatattaa
      601 aaacacgggc aaaaccatca ccaaagtagt gagttgtacc acattgtaaa gctttaccat
      661 ctggcataag tgcttcaatt gtaaaagttt gttcagcgcc ggcaaatttt tctttctcgg
      721 ttttagtacc ggttaaaaac ggaatcgcga gtaattcttt acccatgttt tcataaactt
      781 taagcatatc aagcgcatgg gtccgtgctt ctacttccgt ttcatgaatc gtgtgccctt
      841 cttgccataa gaattcacta ccgcgtaaaa aaggacgcgt tgtcttctcc cagcgaacga
      901 cagaacacca ctgattatat ttctttggta aatcacgata tgagctaact attttagcgt
      961 agtgatcaca aaataatact tcacttgtcg gacgaataat aagtggatct tcaatctttt
     1021 gattaccacc ttgtgttgcg actaatgttt cgggggcaaa accttcgata tgatcttttt
     1081 ctttttgaaa aagacttgtg ggaataacaa ggggcatgta cacattatca tgaccttttt
     1141 ctttaaactt ttcatctaaa tatttttgaa tattttccca aatcgcataa ccataaggtc
     1201 ggtaaataat aaaaccttta acttcgctat aatccattaa ttccgccttg cgacacacat
     1261 cagtgtacca ttgagcaaaa tcttcacttt gggttgtaat tgcatcaatt ttaatcttac
     1321 tcatctttat ttcctcttac taaaattaat aattcttagt gctttctttt tatcaatagt
     1381 ttgcaagctg ttgtctttct tgctaatttc atcactagcg acataatcta cacctgcccg
     1441 aatataatac tcgatttcac tccactttgg caaatcacta atcattattg gtttttgatg
     1501 acgtaataat ttacctaatg atgaaagtaa ggataagcgt tcccgttcat cattaactgt
     1561 atgggttaag attttttcat tcaccataaa gtaatcaact tcaccataaa ttttgtctgg
     1621 cagtgttaat tcagtatcac taatgattaa acacacttca tacttttgtt ttttaatttt
     1681 cttaatcagt tttaacatat cttcaatgtt cacaaatgtg ttagcgaaat cttgttcttc
     1741 taacataaag accattttga gatcattcgc aaatggctgc gtactaaaag catcactaat
     1801 aaagtcgact ccacctaacc caagggggac aaatagtaat tgcttattat tgcggcgcat
     1861 attgtaataa cgggccgctg ccttccggag caccatcttt acaagctgtt cgttttgctt
     1921 attttctcgc gcaaattgcg cagcttcatt gaaactcccg aataatgaac cgctgatgct
     1981 gaaggtcgtt aagtacccta atatttcaac tgttttagca ttaacaattg gttgaaataa
     2041 agtatcgact cccttgcgag ccacaagttg tgttagttca ctgcgatata aacttaagtc
     2101 catgtcttta atttctttat ttggatcatg aattaagagc cgaccgtctt ctttaagcgc
     2161 ataaagactt aattcttccg ctgttttaaa aaacatattc atctccgaaa attgacgaga
     2221 ccgcgttaac ccaatcccga gtgaatattc gttgtagcca tttttttgga gataaccgct
     2281 catgcgtttc tttattgatt ggaacacatc aatagcggta tcaacatcac ttgaacgcgg
     2341 gaagtatatg ccaacagtat gattatcaac aaaacgaatg acgcg
//
LOCUS       DAOPCD010000141         2371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_35287_length_2371_cov_4.608808, whole genome shotgun sequence.
ACCESSION   DAOPCD010000141 DAOPCD010000000
VERSION     DAOPCD010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35287_length_2371_cov_4.608808"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PLQ55_03905"
     CDS             complement(<1..640)
                     /locus_tag="PLQ55_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK29106.1"
                     /translation="MIKVKNLHKTYKKGVKAVNGLNFKVKAGEIFGLLGPNGSGKTTT
                     INAILDLVDYETGEIILFDNKYKTKVEAKKHIGLITQEVGVFNELTVRENIDYFCGLY
                     INDEKLRAQYVEEAMDFVQIKDFAKFKPKKLSGGLLRRLHIACGIAHKPTLIFFDEPT
                     VGIDPQSRNHILEQIKVLNKNGATIVYTSHYMEEIEMLCDYILIMDKGQVIAE"
     gene            complement(786..1544)
                     /locus_tag="PLQ55_03910"
     CDS             complement(786..1544)
                     /locus_tag="PLQ55_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HPK29107.1"
                     /translation="MRRNANPIFRNMNNDMNQAMTSSVPVDVTVSTASWSGIALKTLL
                     LVLIMIAAGLSTWFLPFPVVVGFLIFGGIASFFFVFFAMRSVKTAPVLSVLYAITQGI
                     MYGALTFIVETYFPGVGVMALVATFGIVGVMAFLHSIKAVRATPGLIRFVFAALLMVM
                     ISSLVLFIISRVAQDLYLSYVDNYPLMVIVSAFLVVLGAIMLILDFNNAETIVQSGAP
                     KIYEWQVSLGFLVTLVWIYLQVIRFLLVLARNRE"
     gene            complement(1583..2299)
                     /locus_tag="PLQ55_03915"
     CDS             complement(1583..2299)
                     /locus_tag="PLQ55_03915"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK29108.1"
                     /translation="MGRAFQVRAASMASTAAKKSALNMRASKEIFMVAKSGGTDPNSN
                     LALRAALDKYRAMGATKDVMERAIKKAEGDDAFAYIPGRYEVMGPGGSQLVIDTLTDN
                     VNRAISDIKAVLKKTDSILQTVAYNFTETGIFLFAGNNLEEITETLILNDVDVWDINE
                     EDDVIEVLVNPTSYGKTRDTLYSLGIKEFNISEIQMIPNDKVTLNAEDTEVFKRIIDM
                     LEDVQDVQEVYHNVNVPTEE"
BASE COUNT          751 a          498 c          362 g          760 t
ORIGIN      
        1 cttcggcaat aacttggcct ttgtccataa ttaaaatata gtcacatagc atttcaattt
       61 cttccatgta atgcgaagta taaacaattg tcgccccgtt tttatttaat actttaattt
      121 gttctaagat gtgattacga ctctgtgggt caatcccaac ggttggttca tcaaagaaaa
      181 taagcgttgg tttatgcgca atgccgcacg ctatatgtaa tctccggagt aatccgcctg
      241 aaagtttctt tggtttaaac tttgcaaaat ctttaatttg cacaaagtcc atcgcttctt
      301 caacatattg tgcacggagt ttttcatcat ttatataaag tccgcagaaa taatcaatat
      361 tttctctaac tgttaattca ttaaagacac caacttcttg ggtaataagt ccgatatgct
      421 ttttggcttc aactttagtc ttatatttat tatcaaataa aataatttcg cctgtttcat
      481 aatccactaa atcaagaata gcgttgatcg ttgtcgtttt accgctacca ttaggaccta
      541 ataagccaaa aatttctcca gctttaactt taaaattaag accatttaca gccttaacac
      601 cttttttata tgttttgtgt aagtttttta cttttatcat attaatcacc tataagcatt
      661 ataacttcat ttatgtgaat tttaacagta taagcaattc gtaaaggttt ctttacaaag
      721 taaagcgcga caataaagaa aaaacgacca catgtagtgg ccgtcttttt gctaacatat
      781 attccttatt ctcgattccg agcgagaaca agaagaaagc gaatgacttg taaataaatc
      841 cacacaaggg taactaagaa ccctaaactt acttgccatt cataaatttt tggcgctccg
      901 ctttggacga ttgtttcagc attattaaag tcaagaatga gcataatcgc cccaagcacg
      961 actaagaatg cactgacaat gaccatcagc gggtaattat caacataact taaatataaa
     1021 tcttgggcaa cacggctaat aatgaaaagt actaaactac taatcattac cattaataag
     1081 gctgcaaaaa caaaacgaat taagcctggt gtagcgcgta cagctttaat actatgtaag
     1141 aaagccatca caccaacaat gccaaaagtc gccacaagtg ccattacacc aacaccaggg
     1201 aagtaggttt caacaataaa tgtcagtgcg ccatacatta tcccctgagt tattgcatat
     1261 aacacactta aaacaggagc agttttcaca cttcgcattg cgaagaacac gaagaagaat
     1321 gaagcaatac caccaaagat taaaaaaccg acgacgacag ggaatggtaa aaaccatgta
     1381 ctaagtcccg cagcaatcat aatgagtacg agtaataatg ttttaagggc aatgccgctc
     1441 catgatgctg tgctaactgt cacatcaact ggcacactac ttgtcattgc ttgattcata
     1501 tcattattca tattacggaa tattggattt gcatttctac gcatataaag ttcctcctta
     1561 tatttttgcc actaatatta tatcactctt cagtgggcac attaacatta tgatacactt
     1621 cttggacatc ttggacatct tctagcatat caataatgcg tttaaatact tcagtgtctt
     1681 ctgcatttag tgttacttta tcgttaggaa tcatttgaat ttcactaatg ttaaattcct
     1741 taatccctaa agaatataat gtatcgcgtg ttttaccgta tgaagtcgga ttaacaagaa
     1801 cttcaatcac atcatcttct tcattaatat cccatacatc aacatcatta agaattaatg
     1861 tctctgttat ttcttcaaga ttattgcctg cgaataaaaa gatacctgtt tcggtaaagt
     1921 tataagcaac tgtttgtaag atcgagtccg tctttttaag cacagcttta atatcactaa
     1981 tcgcgcgatt aacattgtca gttaaagtat caattacaag ttgactgcca cctgggccca
     2041 tcacttcgta acgccctgga atataagcaa aagcgtcatc tccttccgcc tttttaatcg
     2101 cccgttccat gacatctttt gtcgcaccca ttgctcgata tttatcaagc gctgcgcgta
     2161 aggctaagtt ggaattagga tcagtgcctc cgctttttgc caccataaaa atttcttttg
     2221 aggcccgcat attaagtgca ctctttttcg ctgcggtact tgccatactt gccgctctaa
     2281 cttgaaatgc tcttcccata actttgctcc tttaactact attttattat aagcctaata
     2341 cgtcacttta cgtaaataaa ggccgtctcc a
//
LOCUS       DAOPCD010000142         2368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_35366_length_2368_cov_2.257242, whole genome shotgun sequence.
ACCESSION   DAOPCD010000142 DAOPCD010000000
VERSION     DAOPCD010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35366_length_2368_cov_2.257242"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PLQ55_03920"
     CDS             complement(<1..537)
                     /locus_tag="PLQ55_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HPK29109.1"
                     /translation="MKHFRKLLAKIKKADEKFNLIQSGDKIVIGISGGKDSIVLFEAL
                     RTYKRYSKKDFTLYPVILDLGFSGFDASSYTAFFKEREYDLIVNDSRDVSKILSVQKE
                     KQGLSKLPCSICSRMKKAAINEVANKLGANKVAFAHHIDDALETLFMNMIGGGRFATF
                     SPQMYLERAEITFIRPLIL"
     gene            580..1437
                     /gene="truB"
                     /locus_tag="PLQ55_03925"
     CDS             580..1437
                     /gene="truB"
                     /locus_tag="PLQ55_03925"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPK29110.1"
                     /translation="MNKGKNGILLVNKEPGYTSRKVGNMLSRKLKIKKVGHLGTLDPF
                     ASGLLIFAVGEGTKVLPYLEDEVKTYVATLYFGLLTTTLDPDGEVIKKEKVPPFSKAE
                     IEKVIKQFTGEVTQIPPTTSAIKIDGKRAYEYARKNIEVDMPSRVVEVYTIEVLEYNH
                     PLLVIEATVSKGTYIRTLAQDIALALGTNATTIKLVRTRHGDFSLEKAKLFEEVSEED
                     IIDPSLGLKKFPIYKLSDEEVKRVRNGMTIKLNASEEIVQIHNNSGVLQALYKKKGDN
                     YIALRGFNL"
     gene            1434..2354
                     /gene="ribF"
                     /locus_tag="PLQ55_03930"
     CDS             1434..2354
                     /gene="ribF"
                     /locus_tag="PLQ55_03930"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HPK29111.1"
                     /translation="MIAKHLDLSRITSLAPSTALVVGTFDGVHIGHKYLVEEGFKAAD
                     NVAVLLVYTNKKLTKLHQKSGVLTSLEDRVEIFEKFGVKTVYLLNLGDELLKLEPCEF
                     INKVLVPLAPNSVIVGTDFRFGRGAKGTGEDLREFGKNVFTTRVLAPLYYENEKVSTS
                     LIKELLSKGKPKIAKSLLGRYYALTGLVTKGYGLGNKLGFPTANIALDPSYFVPAHGV
                     YFVRVKFRANYYFGMASLGYHPTVNKVNVPLLEVNIFNFNENIYHDALTVEFIDYLRP
                     EFKFESVDTLIKQLQKDRENCEFKIEKGEY"
BASE COUNT          784 a          375 c          473 g          736 t
ORIGIN      
        1 aagaataagt ggacggataa aggtgatttc cgctctttca agatacatct gtggactaaa
       61 agtcgcgaag cgtccgccac caatcatatt cataaagagt gtttcaagcg cgtcatcaat
      121 atgatgagca aaggcgactt tattagctcc taacttatta gcgacttcat taatcgctgc
      181 ttttttcatt cgtgaacata ttgaacacgg cagttttgat aacccttgtt tttctttctg
      241 aacacttaag attttactga cgtcacgcga atcattaacg attaagtcat attctctttc
      301 cttaaagaaa gcagtataac tactagcgtc aaacccgcta aatcctaaat ctaaaatcac
      361 agggtaaagc gtaaagtctt tcttactata acgcttataa gtccgtagtg cttcaaaaag
      421 aacaatggag tctttcccac cactgatgcc gatgacaatt ttgtcaccac tttgaattag
      481 attaaacttc tcatcagctt tcttaatctt tgctagtaat ttgcgaaaat gtttcattgt
      541 tttaaaacct gttgctttat attataatat aaaagatata tgaataaagg gaaaaacggt
      601 attttattag ttaataaaga gcctggttac acgagtcgca aagtcggtaa catgctttcg
      661 cgtaaactta agataaagaa agtcgggcat ttagggacac ttgacccctt tgctagtggc
      721 ttactcatct ttgccgttgg tgaaggcacg aaagtattgc cgtatttaga agatgaagtt
      781 aaaacttatg ttgcgacatt gtactttggc ttattaacaa cgactttaga tccggatggt
      841 gaagttatta aaaaagaaaa agttccgccc ttttctaaag cggaaattga aaaagttatt
      901 aagcaattta ctggtgaagt aacacaaatt ccaccgacga caagtgcgat taaaattgat
      961 gggaaaaggg cctatgaata tgcgcgtaaa aatattgaag tcgacatgcc aagccgtgta
     1021 gtagaagttt acactataga agtacttgaa tataatcatc ctttactagt catagaggca
     1081 actgttagta aaggcactta tattcgaacg cttgctcaag atatagcgct tgccttagga
     1141 acaaacgcta caacgattaa gctagtgcgc acaaggcatg gtgactttag tcttgaaaag
     1201 gcaaaacttt ttgaagaagt aagtgaagaa gatatcattg atccaagtct agggttaaaa
     1261 aagtttccta tttataagtt aagtgatgag gaagttaaaa gggtgcgaaa cgggatgacg
     1321 attaaactta atgctagtga agaaattgta caaattcata ataatagtgg cgttttgcaa
     1381 gcattatata agaaaaaagg cgataattat atcgcgctac gaggatttaa tttatgattg
     1441 ctaaacatct tgatttaagt cgtattacat ctttagcacc aagcactgca ctagttgttg
     1501 ggacgtttga cggcgtccat atcggtcata agtatttagt ggaagaaggt tttaaagcgg
     1561 cagataatgt tgcggtttta cttgtataca ctaataaaaa acttacaaaa cttcatcaaa
     1621 aaagtggggt gctaacgagt ttagaagacc gtgttgaaat ctttgagaag tttggagtga
     1681 agactgttta tttattaaat ttaggtgatg aattactcaa gcttgaacca tgtgaattta
     1741 ttaataaagt tttagtacct ttagctccaa attctgttat tgttggtact gattttagat
     1801 ttggtcgggg cgctaaaggc actggcgaag atttgcgaga gtttggtaaa aatgtattta
     1861 cgactagagt tttagcgcca ctttactatg aaaatgaaaa agtgagcaca tcactaatta
     1921 aagagctttt aagtaaaggt aaacctaaga tcgcaaagtc attacttggc cgctattatg
     1981 cgctcactgg gttagtgact aaaggctacg gtttaggaaa taaattagga tttccgactg
     2041 cgaatattgc ccttgaccca agttactttg taccagcgca cggtgtatat tttgttcgcg
     2101 ttaagtttag agctaattat tactttggga tggcaagttt agggtaccat ccgacggtta
     2161 acaaagttaa tgtgccacta cttgaagtta atatatttaa ttttaatgaa aatatttatc
     2221 acgacgccct cactgttgaa tttattgatt acttacgtcc agaatttaag tttgaaagtg
     2281 ttgacacgtt gattaagcaa ttacaaaaag atcgtgaaaa ttgtgaattt aagatagaga
     2341 aaggagaata ttaaacgcaa gtatttaa
//
LOCUS       DAOPCD010000143         2359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_35542_length_2359_cov_2.928385, whole genome shotgun sequence.
ACCESSION   DAOPCD010000143 DAOPCD010000000
VERSION     DAOPCD010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35542_length_2359_cov_2.928385"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..>948)
                     /locus_tag="PLQ55_03935"
     CDS             complement(68..>948)
                     /locus_tag="PLQ55_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK29112.1"
                     /translation="NAMLMLAQILIMFGRTSASKSRISQVLNTDTSLEYLINDLSVKA
                     KPSLIVKDVSFKYPGTSDDNYALKNINFTLKAGETLGIIGQTGSGKSTLVHLLARLYD
                     VTDGEIILDDHNVKDYSADNLLDAINIVFQNNMLFSGTIRDNLLWGNKDASDEKINRA
                     LEASLSDEIVNKLANGLNSEVGQAGSLLPGGQKQRIALARALLKRPKILVLDDSLSAI
                     DTINEGKIRTKLRKLYPQLTLIIVAQRVSAIKDADHILVLHEGEIHGAGTHNSLVEND
                     EIYHDIYTTQLKGAKA"
     assembly_gap    949..958
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<959..1808)
                     /locus_tag="PLQ55_03940"
     CDS             complement(<959..1808)
                     /locus_tag="PLQ55_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK29113.1"
                     /translation="MKKGLFKYFKVVWKEGILAIFFILFETVLETLVPFMASRVVDVG
                     IANNDLPYIYKWGALMVCSAFLAFFIGIISVRYVARTGQGFGAELRRAQFAKLQSLDQ
                     EARDKFAVSSLITRLTNDTYTIQNAIIIGMRGIFRAPFMITMVLMLSFNMNSKVAIVF
                     ALTLPVIAFAMLVIVFRVRPLYERMQRNFDELNRTLQENFQAIRVVKAYVTEEQEEAK
                     FKAINKMYRKVASNAFMTVSLNSPIMQLGLYSTIIGLLLVGGNLINTGEAKIGEIQGL
                     LTFVLQL"
     gene            complement(1852..>2359)
                     /locus_tag="PLQ55_03945"
     CDS             complement(1852..>2359)
                     /locus_tag="PLQ55_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPK29114.1"
                     /translation="AKVPTKGIFAKLNMKNYEKEVVFLNESEHPLKAVLASAAIPLVF
                     GEKEIEGTRYVDGGLVDNFPFTPALIANANIIISIGLDPKLLPTKPLPGRLHIDFTPL
                     EELGPFPKVSLDFSPEKTQQYFDLGYENAKALIAYLKREKFFWFGGRLKLRKGRLVTL
                     ETLTAKTK"
BASE COUNT          740 a          475 c          369 g          765 t
ORIGIN      
        1 cgttttaata aataactaaa gagccggcgc attgtttgct tcatatgttt aggcttactt
       61 tgctttttca tgcttttgct ccttttagtt gggtcgtgta aatatcgtga taaatctcat
      121 cattttccac aagtgagtta tgcgtacctg ccccgtgaat ttccccttca tgcaacacta
      181 aaatatgatc agcgtcttta atagcactca cacgttgcgc aacaataatt aaagtaagtt
      241 gcggataaag tttccgtaat ttagtgcgaa ttttcccttc attaatcgta tcaatcgctg
      301 ataaagaatc atcaagcact aatatttttg gtctttttag taatgctctt gctaatgcta
      361 ttcgttgttt ttgaccacct ggaagcaaag agcctgcttg cccgacttca ctatttaaac
      421 catttgctaa tttattcaca atttcgtcac ttagacttgc ttctaatgcc cgattaattt
      481 tttcgtcact tgcatcttta ttaccccata gcaagttatc acgaattgtt ccgctaaaga
      541 gcatattatt ttgaaagaca atgttaatag catctagaag attatccgca ctataatctt
      601 taacattatg gtcgtctaaa ataatctcac catcagttac atcataaagc cttgctaaaa
      661 gatggacgag tgttgattta ccgcttccag tttgaccaat aatcccaagt gtttcaccag
      721 ccttaagcgt gaaattaatg ttttttaaag cataattatc atcacttgtg cccggatact
      781 taaaacttac atctttcaca attaaactcg gttttgcttt aacacttaaa tcattaatca
      841 agtattcaag actagtgtca gtgttaagta cttgactaat tctacttttt gaagcgctag
      901 tgcgaccaaa cataattaat atttgcgcaa gcatcagcat cgcatttann nnnnnnnnag
      961 agttgtaaaa caaaggttag taacccttga atttcaccaa ttttagcttc gccagtatta
     1021 attaaattac caccgacaag cagaagacca ataatcgttg aataaagtcc aagttgcata
     1081 atcggactat tgagtgatac tgtcataaac gcattactag cgactttacg atacatctta
     1141 ttaatcgcct taaactttgc ttcttcttgc tcttccgtaa cataagcttt aacaacacgg
     1201 atcgcttgaa agttttcttg taaagtacgg tttaattcat caaaattacg ttgcatccgt
     1261 tcataaagtg gtctaactcg aaaaacaatg actagcatcg caaacgcaat aacaggaagc
     1321 gtaagtgcaa atacaattgc tactttagaa ttcatattga aactaagcat taataccatc
     1381 gtaatcataa atggagcacg gaatatcccg cgcatcccaa taatgatggc attttgaata
     1441 gtgtaagtat catttgtaag ccgagtaatt aaggaagaca ctgcgaattt atcacgcgct
     1501 tcttggtcta aactttgtag tttcgcaaat tgagcacgac gtaattccgc cccaaatcct
     1561 tgaccagtac gagcgacata acggacacta atgatgccaa taaagaaagc aagaaaagcc
     1621 gaacatacca taagggcacc ccatttatag atataaggta aatcattatt agcgatacca
     1681 acatcaacaa cgcgacttgc cataaatggc actagtgttt ctaaaacagt ttcaaataaa
     1741 ataaaaaaga tggcaagaat cccttctttc catactactt taaaatattt gaataatcct
     1801 tttttcatag cattattatg ccagtaaaat ctctttttct ctatatataa gctacttagt
     1861 ttttgctgtt aatgtttcta acgttactag gcgtcctttt cttagcttta gtcttccacc
     1921 aaaccagaaa aacttttcgc gttttaagta cgctatcaac gccttagcat tctcataccc
     1981 taaatcaaaa tattgctgtg ttttttcagg actaaaatca agcgacactt taggaaatgg
     2041 tcctaactct tctagcgggg taaaatcaat atgtaagcgt ccgggtaatg gttttgttgg
     2101 taaaagtttt ggatctagac caattgaaat aattatatta gcgttagcga taagcgctgg
     2161 tgtaaacggg aaattatcga ctaaaccgcc atccacataa cgagtgcctt caatttcttt
     2221 ttccccaaag acaagcggaa tagcagcact tgctaaaaca gcttttagcg ggtgttcact
     2281 ctcatttaaa aagacaactt ctttttcgta atttttcata tttaatttag cgaaaatccc
     2341 tttagtgggc acttttgct
//
LOCUS       DAOPCD010000144         2357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_35576_length_2357_cov_4.125109, whole genome shotgun sequence.
ACCESSION   DAOPCD010000144 DAOPCD010000000
VERSION     DAOPCD010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35576_length_2357_cov_4.125109"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..153
                     /locus_tag="PLQ55_03950"
     CDS             7..153
                     /locus_tag="PLQ55_03950"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPK29115.1"
                     /translation="MERTFVDKIFALCDYHLLNKYERNSRHLYDLHMIWESGLLKKNT
                     SITY"
     gene            423..1424
                     /locus_tag="PLQ55_03955"
     CDS             423..1424
                     /locus_tag="PLQ55_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29116.1"
                     /translation="MKFKKLLLLPLAALLLNGCAIGKKVDKDVANERVSEAIQTQEEM
                     DVDSLFQEAKQDMTESVSQTNLGAETTSKVTNKGTTTLKAKDLLGENPQVVASAKATA
                     KIDKNGQEDSFVSVNENMYYVDGWFYNDYDNKERGNISLAPKSGKGKIKINVGNIDFA
                     NKNVLAVFSELSGTAVNFGPVDYSQILLLASITGNVKAREFAGVLTITYNLSKDDLVR
                     FVAAQMAGIDYGDLTSEERELVDAIISEIIAQLEKGYEVRKYVIEVKIAKEGYITKVR
                     VENDVTQTITDPQGMGEDPEYVIVVKSKGFTEINTKLNEKVAIKFPDFSDYVERSLM"
     gene            1564..>2357
                     /locus_tag="PLQ55_03960"
     CDS             1564..>2357
                     /locus_tag="PLQ55_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29117.1"
                     /translation="MKLRNLLWLPIAAMLLNSCALGKKVEPSVAQERALAATKANEEA
                     EIDTQAITSKSEISDTIKSYEGETVVTETITSKFESSFKAKGLHGDTPEFAATYKEMM
                     EEKVDGDETYYVNHEEAFYLKDSWLFGGFSTTVRERGETETEENKGKVKIVPIDFKVV
                     SVFDYLSGFSAGEYQFGAPNEEGLAILFQVASEIEAKELFGKLTITYKLDVKSLVLIM
                     LLAFGLDLDAMTEEEREAFDTELNTMVEEMSKVMSINKYQMTLGIS"
BASE COUNT          885 a          326 c          446 g          700 t
ORIGIN      
        1 caatctattg aaagaacttt tgttgataaa atatttgcgt tatgtgatta tcatttactt
       61 aataaatatg agcgaaattc aagacatctc tatgatcttc atatgatatg ggaaagtgga
      121 ttattaaaaa aaaatacttc catcacttat tgataatgta atagcggaaa gacaaaaata
      181 ccctgaatac aatccatctg ttagcgatgg tcaaaaacca cgccagctat taatgaatat
      241 aattgattcc gatgtatata aaacagattt taataaagtg acaacaaaac tgttgtttca
      301 aaaaacaact tatgaaacat gtaagaatac gctttaccaa ataatacttt cagaattggt
      361 ccccgaaatc ataaataaat aagtgctata attaaactaa tacatgaaag gaaacattct
      421 ttatgaaatt taaaaagtta ttattgctcc cgttagcggc attattactt aatggctgcg
      481 caattggtaa aaaagttgat aaagatgtcg caaatgagcg tgtgtcagaa gcaatacaaa
      541 cacaagaaga aatggatgta gacagtctct ttcaagaagc aaagcaagat atgactgaaa
      601 gtgtttcaca aactaaccta ggtgcagaaa caacatcaaa agttactaat aaaggtacaa
      661 caacacttaa agcaaaagac ttattagggg aaaatcctca agttgtagca agtgcaaaag
      721 caacagctaa aattgataaa aatgggcaag aagattcgtt tgtatcagtt aatgaaaata
      781 tgtactatgt tgatggatgg ttttataatg attatgataa taaagagcgt ggtaatattt
      841 cactagcgcc aaagagcggg aaaggcaaaa taaaaataaa tgttggtaac attgattttg
      901 ctaataaaaa tgtccttgct gtgtttagtg aactatcagg cacagcggtt aattttggtc
      961 cagttgatta tagtcaaatt ttacttttag caagtattac tggaaatgtt aaggctcgtg
     1021 aatttgctgg tgttttaacc attacatata atttatccaa agatgatctt gtgcgatttg
     1081 ttgctgcaca gatggcgggt attgattatg gtgatttaac gagtgaagaa cgtgaattag
     1141 tagatgccat aattagcgag ataattgctc aacttgaaaa aggttatgaa gttcgcaagt
     1201 acgtaatcga agttaaaatt gctaaagaag gttatattac taaagtacgt gttgaaaatg
     1261 atgtaacaca aactataact gatccgcaag gtatgggtga agatcctgaa tatgtcatag
     1321 tagtgaaatc aaaaggattt accgagatta atacaaaact taatgaaaaa gtagcaatta
     1381 agtttccaga ttttagtgat tatgttgaac ggtcattgat gtaaataaat aatgtcatta
     1441 gaagagtcgc cgctgtgcga cttttttatt aatcaaaatt gttattttta tatgtttctt
     1501 tacaataaaa agaaaaatac aatgctataa tgtatgaata gtagaaagga ggatactttt
     1561 actatgaaac taagaaattt attatggctc ccaattgcgg caatgttact aaacagttgt
     1621 gcacttggta agaaagtcga accaagcgtt gcgcaagaac gcgcccttgc cgcgactaaa
     1681 gcaaatgaag aagcagaaat tgatacgcaa gcgattacga gcaaaagcga aatttcagac
     1741 acaattaaaa gttatgaagg tgaaactgtt gtaactgaaa caattacttc aaaatttgaa
     1801 tcgtcattta aagcaaaagg cttacatggt gatacaccag aatttgccgc aacttacaaa
     1861 gaaatgatgg aagaaaaagt tgatggcgat gaaacatatt atgtaaacca tgaagaagca
     1921 ttttatttaa aggatagttg gctctttggc ggtttctcta cgactgttcg tgaaagaggc
     1981 gaaaccgaaa ctgaagaaaa caaaggaaaa gttaaaatag ttccaattga ctttaaagtt
     2041 gtttccgtgt ttgattactt atctggcttc tcggcaggtg aatatcaatt tggtgctcct
     2101 aatgaagaag gacttgctat actctttcag gttgcttcag aaattgaagc caaagaactt
     2161 tttggcaaac ttacaattac ctataagtta gatgttaaat cactcgtgct tattatgtta
     2221 ttagcctttg gtctagattt agatgcaatg actgaagaag aacgtgaagc atttgacact
     2281 gaacttaata caatggttga agaaatgagt aaagttatgt caattaataa ataccaaatg
     2341 acccttggta tttctaa
//
LOCUS       DAOPCD010000145         2308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_36588_length_2308_cov_2.295606, whole genome shotgun sequence.
ACCESSION   DAOPCD010000145 DAOPCD010000000
VERSION     DAOPCD010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36588_length_2308_cov_2.295606"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PLQ55_03965"
     CDS             complement(<1..578)
                     /locus_tag="PLQ55_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29118.1"
                     /translation="MGNENLMLQHESKHYIFNYYAGSKAAEDILLIAEHQQACYTHIC
                     KVLGVEPKFKIKYFLCNSPEEVGKIYGDNEPCNAFTSSPDSIYAVYNDDIQCIGFHED
                     AHIISYTINRPESAAIREGLAMYFDRVWWGISNEDWTLYYLKTNKYISICNLLDNKYF
                     FSEDCSLTYPIMGAFTNYLIVHYGIKKYLVFY"
     gene            complement(1408..1719)
                     /locus_tag="PLQ55_03970"
     CDS             complement(1408..1719)
                     /locus_tag="PLQ55_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29119.1"
                     /translation="MNQLFTIVTTFFIDYLPNLNYKNKMIDQLNDQLNDSISDVGLEI
                     LRLINNNPGINAQTIFKIMTQTNEKITIDIVRNSIRRELSNYVEHRGSKKTGGYYFKL
                     P"
     gene            complement(2050..>2308)
                     /locus_tag="PLQ55_03975"
     CDS             complement(2050..>2308)
                     /locus_tag="PLQ55_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPK29120.1"
                     /translation="EQDLTFKYFEQVCEEKQIEVNTKLFIGLNLMRPKGQFTNLGLLI
                     SDQSPIAVKIAEYDDEMNFKLKKTIKGSLLKALIETQEQTE"
BASE COUNT          737 a          375 c          308 g          888 t
ORIGIN      
        1 ctgtaaaaga ctaaatattt tttaattccg tagtgcacta taagataatt agtaaaagca
       61 cccataattg gataagttaa tgaacaatct tcactaaaaa agtacttatt atcaagtaaa
      121 ttacaaattg aaatatattt atttgttttt aagtagtata gtgtccagtc ttcattactt
      181 atcccccacc atacacggtc aaaatacatt gctagtcctt cacgaattgc cgcgctttcg
      241 ggacgattta ttgtgtaact aataatatga gcatcttcat gaaaaccaat acactgaata
      301 tcgtcattat ataccgcata aattgagtca ggcgaactag taaaagcgtt acacggttca
      361 ttgtcaccat atatcttccc aacttcttca ggcgaattac ataaaaagta tttaatttta
      421 aacttaggct caacccctaa aactttacaa atatgtgtgt agcaagcttg ttgatgctcc
      481 gcgattaaaa ggatatcttc tgcagcttta ctacccgcat agtaattaaa gatatagtgt
      541 ttgctttcat gttgtaacat taagttttcg tttcccattt gtcgtaaact tttaaggcgt
      601 cctttcgcct catcttattg ctcataagtt agtgctttaa ttcttagcta attatactac
      661 gataaacgct tcttactctt atttagttaa caatctctta actaatagta ttatttaaac
      721 tttattaaat aacatcacta cttgacttaa tttgcgaaat taaggaaaat aacgctggtg
      781 atagccgctg attacgctta ttgggagcta atcactccag agcccttgga ctgttcaagg
      841 gcatttttta aattctaaca ctttccaatt attaataact tatagtaaat taaaattaat
      901 tagtcattta atttgtcatt aattggtaat tgaattgact taaatgaaaa aaataggtaa
      961 attaatattg ggcatacgat gtaatgcgcg aattaagttt cgactcatct gtgccttttt
     1021 attttgcttg aaataacaaa taatctaact taataattcg ttagtgatat cgactctgtg
     1081 gcgaccaaag cccaattaag taagttttac actaactatt ttatttagat aattattatt
     1141 ccgattacca acaatttgta tatcaaaata cagcaccatc tggaacacaa tgaataccaa
     1201 taaaatcact atctgtcact attatttaca tataatccac taattttaac ttttatagta
     1261 aatccatttc taaacttgat actgttatta gttgaatttt gattgtctaa ccttatttat
     1321 ttacagtttt taatagttaa attttatgct attctttcat atttttttac tattaatgat
     1381 taacaattgg ctttggttat aaaatcttta tggtagtttg aaataatatc cacctgtttt
     1441 tttagagccg cgatgttcta catagttact taattcacgc cttatagaat tccttacgat
     1501 atcgattgtt attttttcat tcgtttgagt cattattttg aaaattgttt gtgcatttat
     1561 acctggatta ttgtttatca accttaatat ttcaagacct acatcactta ttgaatcatt
     1621 taattggtca tttaattggt caatcatttt atttttataa tttaaattag gcaaataatc
     1681 aataaaaaaa gttgtcacta tcgtaaaaag ttggttcatg tccagtacct tcataagctt
     1741 tgatagtccg tggaatacca gttccaaaat tttcaatgta accaagttta tctaaaaggt
     1801 ggacaagtct ttcatttcgt tatgtttgta caccttttaa aacatcatcc aatgtcgtct
     1861 tataaagacc gccaggactt gtgattttaa ctttatcctt ataaaattct attttgatat
     1921 ttgagcggat aaaaaatctg tgtgacaaaa cgcattgaga attatttccc gtagcgaatt
     1981 tccgggataa gatgttgttt caattctttt ccaagatttg gtatcaataa ttgcggttat
     2041 atcatttaat cattctgttt gttcttgtgt ctctatgagg gcttttaaca atgatccttt
     2101 tattgttttt ttaagtttaa aattcatttc atcatcatat tcagcaattt taaccgctat
     2161 tggtgattga tccgaaatca atagtccaag attagtgaat tgcccctttg gtctcattag
     2221 gtttaaacca ataaataatt tggtattaac ctcaatttgt ttttcttcac aaacttgttc
     2281 aaagtattta aaagtaagat cttgttct
//
LOCUS       DAOPCD010000146         2304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_36663_length_2304_cov_3.888839, whole genome shotgun sequence.
ACCESSION   DAOPCD010000146 DAOPCD010000000
VERSION     DAOPCD010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36663_length_2304_cov_3.888839"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..959)
                     /locus_tag="PLQ55_03980"
     CDS             complement(171..959)
                     /locus_tag="PLQ55_03980"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPK29121.1"
                     /translation="MYRPIKRTLDFVLSLLAIIILSPLLLFIAILVRLKLGGPVLFKQ
                     KRVGRYNTIFTLYKFRSMKNAYDEKGELLPDEQRLTKFGIFLRRTSLDELPQLFNILF
                     GKMTIIGPRPKTIFETLLMKDTKFVYRAAIKPGLSSWSVIHGRNNMPNDKALMYDFEH
                     VIKFSAWLDTKIFFKTIGLVLRGSGINTDGHATFLHLSDFLIEKKIKTPEEIEKVRQE
                     AIIIEKENMKVVPMGIIKEDFKAKRREIQKNRKSYTPNQVITWD"
     gene            complement(1018..>2304)
                     /locus_tag="PLQ55_03985"
     CDS             complement(1018..>2304)
                     /locus_tag="PLQ55_03985"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPK29122.1"
                     /translation="TISQWMLRIKEMIGKGIVDVYAENALNEALLDLDVRCVDYKYNF
                     VEEIDTLEDYERVSNDVRLFEFLNQKVYHGDYLIKLIEIIKKHNFKRPFLVYNSATFK
                     NTEFYNYMKNLALTPYNSYSSNPKYEEIIPGLELFKRNKCDVIVAIGGGSAIDVAKAI
                     KLFSGLNPNINYLEQKLVYVNTPLIAIPTTAGTGSETTRYSVIYVDGEKYSLTHDCIF
                     PNIAVIDPTFLRTLPEYHRRSSLLDALCQAIESFWSKGSIEETRLLSARGIHLFRYTY
                     KHYLEGHPFVDDRIMKVSYLAGQAINITATTAPHAMSYKLTSLTGIAHGHAVSVTLPY
                     VYKYMLEIAKKSEDKELKQTFVNLAKIFETSETKLFEVILNIFNEFELEKPTVTEDQL
                     IELINDVNEERLQNNPVLLDKEAIEEIYRSALIVKK"
BASE COUNT          767 a          397 c          310 g          830 t
ORIGIN      
        1 aagaagtaat atcatcaagc actaatatta caaagtaatc ttttaataat ttatccggca
       61 taaaagtcct cctaaaaagt tggattcata gacattatac tataaagtaa aagaatatta
      121 tatttttaag ggtcttcttt atttttataa aataaaaagt gttactaaca ctagtcccaa
      181 gttatcactt ggttaggagt atatgattta cgattttttt gtatttcgcg acgtttagcc
      241 ttgaagtctt ctttaataat tcccatcggt acaaccttca tattttcttt ttcaataata
      301 atcgcctctt gtctaacttt ttcaatttct tcaggggttt taatcttctt ttcaattaga
      361 aaatcactta aatgtaaaaa tgtagcgtgc ccatcagtgt taatccctga accgcgtaac
      421 actagaccga ttgtcttaaa aaatatttta gtatctaacc atgctgaaaa tttaataaca
      481 tgttcaaagt cgtacatcaa tgctttatcg ttaggcatat tattacggcc gtgaattaca
      541 gaccaactac taagtcctgg tttaattgcc gcacgataca caaacttcgt atctttcatt
      601 aatagcgttt caaaaatagt tttaggacgg ggaccaataa ttgtcatttt tccaaacaaa
      661 atattaaaaa gttgtggtaa ttcgtctaga cttgtacgtc ttaaaaatat cccaaacttt
      721 gttaatcttt gttcgtctgg caacaattca cctttttcat cataagcgtt cttcattgac
      781 ctaaatttat ataaagtaaa tattgtatta taacgaccca ctcttttttg tttaaagaga
      841 acagggccac ctaattttaa acgcactaag atagcaatga ataataaaag cgggctcaat
      901 ataataattg caagtagcga taatacgaag tctaatgttc ttttaattgg tctatacata
      961 attaattacc atgcacacat agtgtttaca ttttaccata tgtaatcatc acatgtctta
     1021 tttcttaact ataagtgcac tcctataaat ttcttcaatt gcttctttat ctaacaaaac
     1081 ggggttattt tgtaatcttt cttcgtttac atcattaatc aattcaataa gttgatcttc
     1141 agttactgtt ggtttttcta attcaaattc attaaaaatg tttagtataa cttcaaaaag
     1201 ttttgtctcg ctagtttcaa aaattttagc aagattgaca aatgtttgtt taagttcctt
     1261 gtcttcgctt tttttagcaa tttctaacat atatttataa acatacggta atgtaacact
     1321 tacagcatga ccatgggcaa taccagttaa tgatgttaat ttatagctca ttgcatgtgg
     1381 agcggtagtc gcagtaatat taattgcttg gcccgctaag tatgaaactt tcataatacg
     1441 atcgtctaca aaagggtgac cttctaaata atgtttataa gtatacctaa ataaatggat
     1501 tccccttgca cttaatagcc tagtttcctc tatagaacct tttgaccaaa aactctcaat
     1561 cgcttgacat aatgcatcta ataatgagga gcgacggtga tattctggta atgtgcgtaa
     1621 gaatgtcggg tcaataacag cgatatttgg aaaaatacaa tcgtgtgtta atgaatattt
     1681 ctcaccatca acataaataa ccgaatacct tgttgtttca cttccagttc ccgctgttgt
     1741 aggaatagca ataagtggag tattaacata aactagtttt tgctctaaat aatttatatt
     1801 cggattaaga cctgaaaata acttaattgc tttggcaaca tcaatggcag aaccaccccc
     1861 aatcgcaaca attacatcac atttatttct tttaaataat tctaatcccg gaattatttc
     1921 ttcatattta gggtttgaag aataactatt gtatggagtt aaagcaagat ttttcatgta
     1981 attataaaat tcagtgtttt taaaagtagc gctattataa acgagaaatg gtcgtttaaa
     2041 gttatgtttt tttattattt ctataagttt aatcaaataa tcgccgtgat atactttttg
     2101 attcaaaaat tcaaaaagcc taacatcatt actaactctt tcgtaatctt ctaaagtatc
     2161 aatttcttcc acgaaattat acttataatc aacgcatcgc acatctaaat caagtagtgc
     2221 ttcatttaaa gcattttctg cataaacatc aacaataccc ttaccaatca tttccttaat
     2281 acgaagcatc cattgactaa ttgt
//
LOCUS       DAOPCD010000147         2292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_36929_length_2292_cov_4.074207, whole genome shotgun sequence.
ACCESSION   DAOPCD010000147 DAOPCD010000000
VERSION     DAOPCD010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36929_length_2292_cov_4.074207"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLQ55_03990"
     CDS             complement(<1..253)
                     /locus_tag="PLQ55_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPK29123.1"
                     /translation="MKGIILYFSKYGATKKYVDWLSKSTNFDVVPVKKAKKSHIKDYD
                     VIILGSGIYASGIKGVKFLRKHAKLLSDKKVLIFCVGASP"
     gene            complement(255..1580)
                     /locus_tag="PLQ55_03995"
     CDS             complement(255..1580)
                     /locus_tag="PLQ55_03995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPK29124.1"
                     /translation="MNNYDVIIVGAGTAGTYFAYLLGKRGLKVLVIDKDKEKDLAKRL
                     DIFHFTTESFARFNLPESKAGDEEFVSKFSYIYSRSALDNYEKKNFLEVSVMHLPLFI
                     KRLRNKAIKENVEFKFATEFKDLIYDAKGRISGIETKDEKSYNARLVVDASGIPSVVR
                     RVVKDPYMETFPIGPRDKFYVFLKYVKLKDPNIKIDMSLSWPYYKSWIAPQQNKDGAI
                     VGVGANISVDYARKCMKKFEDSIKLPAYDLQYEEWGATPYRRPPYSFVSDGFLVLGDA
                     ACLTQPFNGEGITAAWVQSTPAADVVANALKDDKYPTKEALWPINVMYQTGEGALFAG
                     QRALLVGAVNMSKEDNDYLYANGIIFKSDDEEVKGSVAGNLIKGVLKGKFSFAALRSL
                     MGASSTGNKLTKHYKNYPTSPEKYFAWKKKADRLWKKAGSMADNVKDAD"
     gene            complement(1713..2291)
                     /gene="pduL"
                     /locus_tag="PLQ55_04000"
     CDS             complement(1713..2291)
                     /gene="pduL"
                     /locus_tag="PLQ55_04000"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HPK29125.1"
                     /translation="MKKILIETSARHIHLSEKDLKILCGEDATLEVRGPLSQPGQFST
                     TTRLTIVGAKRNIERVTVLGPTRKDTQVEVSLTDAFTLGLDVPVRESGDIKGTPGVKL
                     VGPAGEVTLEQGLIVAKRHIHMTPADAKEFNVKNGEVVGVRVKTCDRSLVFEDVVVRV
                     REDFALAMHIDTDEANAAGICGEVYGEIVKLA"
BASE COUNT          667 a          496 c          370 g          759 t
ORIGIN      
        1 acggagaagc accaacacaa aaaattaata cttttttatc gcttaataat ttagcgtgtt
       61 tacgtagaaa cttaacacct ttaatccctg aagcataaat gccgctacca agaataatta
      121 catcataatc tttgatatgg ctctttttag cttttttaac tgggacaaca tcaaagttag
      181 tactcttgct tagccaatcg acatattttt tggtcgctcc gtatttagaa aagtagagaa
      241 ttattccttt catcttaatc agcatccttt acattatccg ccattgaacc tgcttttttc
      301 cacaagcgat cagctttttt cttccacgcg aaatattttt ctggtgaagt aggatagttt
      361 ttataatgtt ttgttaattt gttgcccgtg ctactagcgc ccattaaact acgaagtgcc
      421 gcaaacgaga actttccttt taagacaccc ttaattaagt tacccgctac acttcctttt
      481 acttcttcat catcactctt aaagataatg ccgtttgcgt ataagtaatc attatcttct
      541 ttactcatat taacagcccc gacaagaagc gcgcgttgac cagcaaagag tgcaccttct
      601 cccgtttgat acataacatt aattggccat aatgcttctt ttgttggata tttatcatct
      661 tttaaggcgt tggcgactac atctgcggct ggcgtcgatt gcacccaagc agcggtaatg
      721 ccttcaccgt taaaaggttg cgttaagcat gctgcatctc ctaagacaag aaaaccgtca
      781 cttacaaaac tatagggcgg acgccgatat ggggttgcgc cccactcttc atattgcaaa
      841 tcatatgcgg gtagtttaat actatcttca aacttcttca tacatttccg cgcataatca
      901 acggatatat tcgcgccaac cccgacaatt gctccgtctt tattttgttg tggggcgatc
      961 cagcttttgt aataaggcca acttaaagac atatcaatct taatgttcgg atcttttagt
     1021 tttacatatt ttaagaacac atagaacttg tcgcgtggac caattggaaa tgtttccatg
     1081 tatggatctt ttactactct tcttacgaca ctagggattc ctgaagcatc aacaacaagc
     1141 cgagcgttat aacttttttc atcctttgtt tctatcccgc ttattcgtcc tttagcgtca
     1201 taaattaaat ctttaaactc tgtagcaaac ttaaattcaa cattctcttt gattgcctta
     1261 ttgcgtagtc ttttaataaa taaaggcaaa tgcatgacgc ttacttctaa gaaatttttc
     1321 ttttcataat tatcaagcgc tgaacgcgaa taaatataag aaaactttga gacaaactct
     1381 tcatcacctg cttttgactc cggtaaatta aagcgggcaa aagattctgt agtaaagtgg
     1441 aaaatgtcta agcgtttcgc taaatctttt tctttatctt tgtcgattac aagtactttt
     1501 agcccgcgtt tccctagtaa ataagcaaaa taagtgccag cggtacctgc cccgacaata
     1561 attacatcat aattattcat atgtatccct ccatttttat tgtgacatat ttattactaa
     1621 aagtaattag tgtttacgat aattttaaaa ttataaacaa aaaaacatct cgtgaagtag
     1681 atgtttaatg ttttaataat aattagatta atctaggcaa gttttacaat ttcgccataa
     1741 acttcgccac agatacccgc tgcgttagct tcgtcagtgt caatgtgcat agcaagcgca
     1801 aagtcttcac gaacccgcac gacaacgtct tcaaacacaa gactacgatc acatgtttta
     1861 acacgaacgc cgacgacttc accattttta acattgaact cttttgcgtc agctggtgtc
     1921 atatggatat gacgttttgc cacgattaaa ccttgttcta aagtaacttc acctgctggg
     1981 ccaacaagct taacacctgg ggtgccttta atgtcaccac tttcacgcac tgggacatct
     2041 agtcctaaag taaaagcatc agttagtgaa acttcaactt gcgtatcttt acgcgttggt
     2101 cctaacacag taacgcgttc aatattacgt ttcgcaccaa cgattgttaa acgtgttgtc
     2161 gtggaaaatt gcccgggttg actaagcggt cctctaactt ctaaagtagc gtcttcacca
     2221 cataatattt ttaagtcttt ttcacttaaa tgaatgtgac gtgcagatgt ttcaataagt
     2281 atttttttca tg
//
LOCUS       DAOPCD010000148         2274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_37288_length_2274_cov_3.824245, whole genome shotgun sequence.
ACCESSION   DAOPCD010000148 DAOPCD010000000
VERSION     DAOPCD010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37288_length_2274_cov_3.824245"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PLQ55_04005"
     CDS             <1..539
                     /locus_tag="PLQ55_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPK29126.1"
                     /translation="KVLREALVKDAADENSSHDFGKNIIPTLLANNNRLFVHRFDGYW
                     KDVGTIDSLWEANMDLIEMMDEDFYQADKHYFRLFSEDTNSVPQYIGKKAKVTNSIVN
                     QGAIILGEVEGSIISNEVFIEVGAVVKDSVIMPGVTVGRNAYVNKAIVANDLELPEHS
                     KYNEAGEKVLLIHEGVVQ"
     gene            536..1651
                     /gene="glgD"
                     /locus_tag="PLQ55_04010"
     CDS             536..1651
                     /gene="glgD"
                     /locus_tag="PLQ55_04010"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HPK29127.1"
                     /translation="MKVLAYLNLHHAPRLGPLTTRRSTAVVSFLGRYAIMDFMLSNFS
                     NSGFDRIATLVDNHPDSVLKHFGSRNTFNINTKLGLEIIAYNEGGSKAGPRYNTDIAN
                     IVFNDWILEQADPNYVVVAPSYFILPIDFRPILKQHIAKKSKVTAVYQRVNNARNEYI
                     GRAIFDLNKDGHVINISRNKGEKDARNIGLEIYIFNRETFDFLIRDAQNISSFYSLSD
                     IVAYHALKDIRVDTYEYVGYMRCIDSYQHYYDYSFELLNYRIRRQLFLHNWPIYTVTH
                     DTPPAKYEATADVKNAFIANGSLISGMIKNSIISRNVKIEEGAVVENSIIFTDSIVRR
                     NKVISHAIIDKKAEVAVAAELAGTPEDILYVRKGDRV"
     gene            1651..>2274
                     /locus_tag="PLQ55_04015"
     CDS             1651..>2274
                     /locus_tag="PLQ55_04015"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPK29128.1"
                     /translation="MKILMVASESSPLAKTGGLADVVYALSKELAILGEEVSVVMPFY
                     GNIKRSPDQEIRFVANVQVPLGWRFQNAKVYKTFIDGITFYLLENEYYFGREGFYGYD
                     DDTERFAFFSVAVRNMMQALKIKPDVVHVHDYHGGMVPALIKIQNSRTKFFNKMKFVV
                     TIHNPAFQGEFHPDLLPDFYDIRKEYFDDGTLRYRDKVNALKAAIVFA"
BASE COUNT          710 a          370 c          476 g          718 t
ORIGIN      
        1 ataaagtatt acgcgaagca ctcgttaaag atgccgctga tgaaaatagt tcccatgact
       61 tcggtaaaaa tatcattcca actttattag cgaataataa tcgcttattt gtccatcgct
      121 ttgatgggta ttggaaagat gttggtacga ttgattcatt atgggaagca aatatggatt
      181 taattgaaat gatggatgaa gatttctatc aagccgataa acattacttc aggcttttta
      241 gtgaagacac aaatagtgtg ccgcaataca ttggtaaaaa ggcgaaagtc acgaatagta
      301 ttgtcaatca aggcgccatt attttaggtg aagttgaagg atcaattatt agtaatgaag
      361 tttttattga agtaggggca gttgtaaaag acagtgtcat tatgcctggc gttactgtcg
      421 ggcgtaatgc ttatgttaat aaagcaattg tcgctaatga tttagaactt ccagaacatt
      481 ctaaatataa cgaagcgggt gagaaagtat tactcatcca tgaaggagta gtgcaatgaa
      541 agttttagct tatcttaatt tacaccatgc cccacgcctt ggaccattaa cgacgcggcg
      601 ttcgactgct gttgttagtt ttttagggcg ttatgcgatt atggacttta tgcttagtaa
      661 ctttagcaat agcggatttg accggattgc aacactagtc gataatcatc ctgattcagt
      721 attaaaacat tttggtagtc gtaatacttt taacattaac actaagttag ggttagagat
      781 tattgcttac aacgaaggcg gttcgaaagc tggtccgcgt tataacacgg atatcgctaa
      841 tatcgtcttt aatgactgga ttttagagca agctgatcca aattatgttg tcgttgctcc
      901 aagttacttt atattaccaa ttgattttag gccgatttta aagcaacata ttgctaaaaa
      961 atcaaaagta acagcggtat atcaacgcgt taataatgcg cgaaatgaat atattggacg
     1021 cgctatcttt gatttaaata aggacggtca tgttattaat atttcacgaa ataaaggtga
     1081 aaaagatgca cgcaatatcg gacttgaaat ttatattttc aatcgtgaaa catttgattt
     1141 cctcattcgt gatgcccaaa acattagctc attttattcc ttaagtgata ttgttgctta
     1201 ccatgcctta aaagatataa gggtcgatac ttatgagtat gtcggatata tgcgctgtat
     1261 tgatagttat cagcattatt atgattatag ttttgaatta cttaactatc gtatccgtcg
     1321 tcaactcttc ttacataatt ggccgattta caccgtaacg catgataccc caccagcgaa
     1381 gtatgaagca acggctgatg tcaaaaatgc gtttatcgcc aacggatcat taatcagcgg
     1441 gatgattaaa aattcgatta tttcacggaa tgtcaagatt gaagaagggg cggttgtgga
     1501 aaattccatt atctttaccg attcaatcgt tagacgcaat aaagtgatta gtcatgctat
     1561 tattgataag aaggcggaag tggcggtggc tgctgaacta gcgggtacgc cggaagatat
     1621 actatatgtt agaaaaggag atcgagtgta atgaaaatat taatggtagc aagtgaaagt
     1681 agtccacttg caaaaactgg tggcttagcc gatgtcgtct atgccttaag taaggaactt
     1741 gcgattcttg gggaagaagt aagtgtagtg atgccatttt acggcaatat taaacggagt
     1801 cctgatcaag aaatccgctt tgtcgcgaat gtccaagtcc ctttaggctg gcgctttcaa
     1861 aacgctaaag tgtataaaac atttattgac ggtattacct tttatttatt agaaaatgaa
     1921 tactattttg gccgtgaagg attttatggg tatgacgatg atacggaacg ctttgcgttt
     1981 tttagtgtcg ctgttcgcaa tatgatgcaa gcattaaaaa ttaaaccaga tgtcgttcat
     2041 gtccacgatt atcatggcgg catggtacca gcgttaatca aaattcaaaa ttcacgcaca
     2101 aaattcttta ataaaatgaa atttgttgtc acaattcata atccagcatt ccaaggtgaa
     2161 ttccatccgg atttactccc tgatttttat gatattcgga aggagtattt tgatgacgga
     2221 acattacgtt atcgtgacaa ggttaatgcg cttaaagctg cgattgtctt cgct
//
LOCUS       DAOPCD010000149         2255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_37730_length_2255_cov_3.272727, whole genome shotgun sequence.
ACCESSION   DAOPCD010000149 DAOPCD010000000
VERSION     DAOPCD010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37730_length_2255_cov_3.272727"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..890
                     /locus_tag="PLQ55_04020"
     CDS             90..890
                     /locus_tag="PLQ55_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29129.1"
                     /translation="MIKRRPEFDPFRDKKVFRCKFCNFPITNPGLYPGKKIMINRYCP
                     ACHSNNTFALLNKQDQTFLRMGFFSNPLYPISPHHLKGVPLKFSLIVFPLSVFFNFVA
                     NIVYMPFGLIYNNIQIKKALKEFYASYKDQDTNLILIDMLEKKAGPKNQYASYKTGIA
                     YLEGIHVPYDLEKAESYFKIAKEEHEGGLVKLAETLIRSENPNYDKAFTILNLVKDNP
                     LASYYLAYLYFYGYGVTPSLRIATIYAGTAHTHNIKDYYDIYPKILQI"
     gene            1045..1206
                     /locus_tag="PLQ55_04025"
     CDS             1045..1206
                     /locus_tag="PLQ55_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29130.1"
                     /translation="MEKLVFETIESLLEIKKLRIEERGPVLSRLTDDEIVLLIDNLVE
                     DLQSEYLVQ"
     gene            complement(1253..2131)
                     /locus_tag="PLQ55_04030"
     CDS             complement(1253..2131)
                     /locus_tag="PLQ55_04030"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPK29131.1"
                     /translation="MIDDKLKTLIILAKVKNYTQTAELCNLSQPAVTQHIKALESRYG
                     IEIFRRNGRNLTLTYEGKSLVDSAKKLIALERNIQKEIKKQKGQPKKLDIGITLTAGG
                     YFIPEIMEVFKDKYPNLRFNFHTDIAENILERMRLNELDFAIIDGTSQTDEFNIELLF
                     RDELIIIGPKDHRLADKKNVSIEELKKEKFILRHEKANTRLAFEKYLSNHFDNINNFD
                     VILEIDNTAMIKQLIIAGHGLSVMSKAICEINMQVGTLKQIDLRDFHLERGIYLVYPK
                     ELENSEIIDSILALKE"
BASE COUNT          739 a          351 c          376 g          789 t
ORIGIN      
        1 aatgataata attagaaaga tttttatata atttacataa taattaatga aatttactat
       61 aatatagtaa gtggaggtaa gagtaactta tgattaaaag aagacctgaa tttgatccgt
      121 ttcgtgataa aaaagtattt cgttgtaagt tttgtaactt cccaattact aaccctggac
      181 tttatcctgg gaagaagatc atgattaatc gctattgtcc agcgtgtcat agcaataaca
      241 cttttgcgct tttaaataaa caagatcaaa catttttacg gatgggtttc tttagtaacc
      301 cgctttatcc aattagccca catcacttaa aaggcgtgcc actgaagttt agtttaatcg
      361 tgttcccctt aagtgtgttc tttaactttg tcgcgaatat tgtgtatatg ccgtttggct
      421 taatttataa caatattcaa attaagaagg cactgaaaga gttttacgct tcttataaag
      481 accaagacac taatttaatc ttaattgata tgttagagaa gaaagctggt ccaaagaatc
      541 agtacgctag ttataagaca ggtatagcgt acttagaagg cattcatgtt ccttatgact
      601 tagaaaaggc ggaatcttac tttaaaatag caaaagaaga acatgaaggc ggcttagtta
      661 aacttgcaga aacactgatt cgcagtgaaa acccaaatta cgacaaagct tttacgattc
      721 ttaatttagt aaaagataat ccgctagcga gttattatct tgcttatctt tatttttatg
      781 gctacggtgt aacgcctagc ctccgtattg cgacaattta cgctggtact gcgcacacgc
      841 ataatattaa agattattac gacatttatc caaaaatctt acaaatctaa aataaaataa
      901 catacattct atgtttgctt tactaattgt aaagactttt ttacaaattt aacactcctc
      961 attactttgg tgagtaagta gtcttatgct ataatgcgtt tgtagcaaaa aagcttatct
     1021 agtggggaga aggagtaaaa gagtatggaa aaattagtgt ttgaaacaat tgaaagttta
     1081 ttagaaatta aaaagttacg gattgaagaa cgtggtccag tattaagtag attaacggat
     1141 gatgaaatcg tcttattaat cgacaactta gtggaagact tacaaagtga atacttagtg
     1201 caataataag tattcatttt tttagtgaag cacggtatat ggccgtgttt ttttattctt
     1261 taagcgctag aatgctatca ataatctcgc tattttctaa ctctttagga taaacaaggt
     1321 aaattccccg ttctaagtgg aagtcgcgta aatcgatttg tttaagtgta ccaacttgca
     1381 tatttatttc acaaattgct ttactcatta cacttaagcc gtgaccggca ataataagtt
     1441 gcttaatcat tgccgtatta tcgatttcta aaataacatc aaagttatta atattatcga
     1501 agtggttact taaatacttt tcaaacgcta ggcgtgtatt agctttttcg tgacgaagaa
     1561 taaacttttc tttttttaat tcttcaatac ttacattttt cttatcagcg agccgatgat
     1621 ctttaggtcc aataataatt aactcatcac ggaagagcag ttcaatatta aattcatcag
     1681 tctgtgatgt gccgtcgatt atcgcaaaat ctaattcatt taagcgcatt ctctctaaaa
     1741 tattttcggc gatatccgta tgaaaattaa agcgtaaatt tggatactta tctttaaata
     1801 cctccatgat ttcgggaatg aagtagcccc ctgctgttaa agtgatgcca atatcaagtt
     1861 ttttaggttg acctttttgc tttttaattt ctttttgaat attacgttca agcgcaataa
     1921 gctttttagc actatcgaca aggcttttcc cttcataagt taaagtaaga ttacggccat
     1981 tacggcgaaa tatctcgatg ccataacgac tttcaagtgc tttaatgtgc tgcgttactg
     2041 cgggttgtga aagattacag agctcggcgg tctgtgtata gttttttact tttgctaaga
     2101 ttataagtgt ttttaattta tcatcaatca tcgtaaattt acctctgttt ctaattataa
     2161 gttattctta tataattata acaaattata agttgttata ttagtaataa gtgtgtataa
     2221 tcataataca acactatata aggaggcaag ttatg
//
LOCUS       DAOPCD010000150         2247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_37922_length_2247_cov_2.992701, whole genome shotgun sequence.
ACCESSION   DAOPCD010000150 DAOPCD010000000
VERSION     DAOPCD010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37922_length_2247_cov_2.992701"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PLQ55_04035"
     CDS             <1..649
                     /locus_tag="PLQ55_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPK29132.1"
                     /translation="LTYKRIYGDWASSNLTKWRSKCIEYNLEQIQQVSYVSGKSTSDF
                     TLVIDAMDILYSDQVDGFVLVSSDSDFTRLVSRLRQNNKFIIGMGESKTPLSLVNSVD
                     LFIYLDKIKNIRDKNVKPKKRGRPAADKKEEESIISLDDLIDVLTNIISETSDENGWA
                     YWSNTNNTLVRKYPGFDPRNYGFKGKALQFFLANGFEKRNEGLDVFIRPKANNEQ"
     gene            complement(724..>2247)
                     /locus_tag="PLQ55_04040"
     CDS             complement(724..>2247)
                     /locus_tag="PLQ55_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29133.1"
                     /translation="GIWSNTPVAPKNKFLSFLALFTFLFGVSVTKTQVARSPQKAEAA
                     EFTVTWNVGEQGYTDGEFAGSKVIDDNFEINFYSGRYLLAPRYFSEGSGYISLQGSGD
                     WLGNGFLISPRPTINNYFINKIVITATDAAYTSKMRTQVGQTMRILFFRTNEFFSGAT
                     YTFEELSKFRYFNLENYAGNEFRIKQISLTYTDVPPAAWVNLTSVTKAKDHYDIKRGV
                     HLLIDKPTIEPSNAFYDYFLTTEDTRLRINGPYVVAEDLCENAVVTINTFGRDAGGER
                     LTTTFTVSVTEAPLTIDGAVGMGYGSIDEKLYRFENVRVQDATLDSDNIVLHASATAT
                     NKINVMHPGFNPLNTYTYVKGGTMSLFAKVRRNTTTGKNFLVEPEVISYTDKAILFAN
                     TIMASDEVGQCVTKYAAVRAIINEMTVEELAKLKNAGVVNGINFSPVRARYVAWALHL
                     GESDPYGDGTPISGMPLVPENSTTQNNNVLLVILLSFSVAVFLYFLTSKRKKLYLKK"
BASE COUNT          716 a          441 c          401 g          689 t
ORIGIN      
        1 tctaacttat aagcgcattt atggcgattg ggcaagtagc aatctgacca aatggcgcag
       61 taaatgtatt gaatataact tagagcaaat tcaacaagtc tcttacgtca gtggtaaaag
      121 cactagtgat tttacactcg taattgatgc aatggacatt ttatatagtg accaagttga
      181 tggctttgtc cttgtcagta gtgatagtga ctttacaaga cttgttagtc gcttacgcca
      241 aaacaataag tttattattg ggatgggtga aagcaagacc cccttatcac ttgtaaattc
      301 cgtcgactta tttatatatc tcgataaaat taaaaacatt cgcgacaaaa acgttaaacc
      361 gaaaaaacgt ggccgtcccg ctgctgataa gaaagaggaa gaaagcatca ttagtcttga
      421 tgacttaatc gatgttttaa cgaacattat cagtgaaaca agtgacgaaa atggttgggc
      481 atactggagc aatacaaaca atacgcttgt tcgtaagtac cccggttttg accctcgtaa
      541 ctacggcttt aaaggtaaag ctttacaatt tttcttagca aacggctttg aaaaacgcaa
      601 tgaaggctta gatgtcttta ttcgccctaa agcaaataac gaacagtaat tattatttgt
      661 aaaagaaaag ctcagtgata cgtaatcacc gagttttttt tgttagtaaa ctacaaataa
      721 ttcctatttt ttaagataaa gtttctttcg ttttgatgtt aagaaatata gaaacaccgc
      781 aacactgaaa gaaagtaaaa taactaaaag gacattgtta ttttgtgtag ttgaattttc
      841 tggcactaaa ggcattcctg aaatcggggt gccgtcgcca taaggatcgc tttcacctaa
      901 atgtaatgcc catgcgacgt aacgcgctct aacaggacta aaattaattc cgttaactac
      961 acctgcattt tttaattttg ccagttcttc gactgtcatt tcattaataa ttgcccgtac
     1021 tgcggcgtat tttgttacac attgaccgac ttcatcactt gccattattg tatttgcaaa
     1081 caaaatagcc ttgtctgtgt aactaataac ttctggttcc actaagaagt ttttacctgt
     1141 cgttgtattt ctccgcactt tcgcaaagag actcatcgtc ccgcctttta cataggtata
     1201 agtgttaagt gggttaaagc cagggtgcat gacatttatt ttattcgttg cggtagcact
     1261 agcgtgtaaa acgatattat cgctatcaag agtagcatct tggacgcgaa cattttcaaa
     1321 gcgatagagc ttttcatcaa tacttccgta acccatgcca accgcaccat caatagtcag
     1381 tggtgcttca gttacactaa cagtgaatgt tgttgttagg cgctcaccac ctgcatcgcg
     1441 cccaaaagta ttaattgtca cgaccgcatt ttcacaaagg tcttcggcga caacatacgg
     1501 gccatttatc cgtaaacgtg tatcttccgt tgttaaaaaa taatcgtaga acgcattact
     1561 aggttcaata gtgggtttat caattaaaag gtgcactcca cgcttaatat cataatgatc
     1621 cttagcttta gtaacactag taagatttac ccaagcagct ggtggaacat cagtgtaagt
     1681 taatgaaatt tgtttaatcc ggaattcatt acccgcataa ttttcaaggt taaaatatct
     1741 aaatttactt agctcttcaa atgtatatgt ggcacctgaa aaaaattcat tagtccggaa
     1801 aaaaagaatc ctcatagttt gaccaacttg tgtacgcatt ttactcgtat aagctgcatc
     1861 agtggcggtg ataacaattt tatttatgaa ataattatta attgtcggtc gcgggctaat
     1921 taaaaaccca ttaccaagcc agtcacctga accttgtaaa ctaatataac cgctgccctc
     1981 actaaaataa cgcggtgcaa gtaaataccg gccagaataa aagttaattt caaaattatc
     2041 gtcaattact tttgaacctg caaactcacc atccgtgtaa ccttgttcac ctacattcca
     2101 agtgacagta aattcagcgg cctcagcttt ttgtgggcta cgtgccactt gtgttttagt
     2161 aacactaaca ccaaaaagaa aagtaaaaag tgctaaaaaa cttaaaaatt tatttttggg
     2221 tgctaccggt gtatttgacc atatccc
//
LOCUS       DAOPCD010000151         2239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_38093_length_2239_cov_3.320055, whole genome shotgun sequence.
ACCESSION   DAOPCD010000151 DAOPCD010000000
VERSION     DAOPCD010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38093_length_2239_cov_3.320055"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PLQ55_04045"
     CDS             <1..537
                     /locus_tag="PLQ55_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HPK29134.1"
                     /translation="NQLTSPFASYPGAGVIFGVTGGVMEAALRTVKALLEKEDAYLID
                     IREVRGFESIKEATLTIDGKELNFAVVHGAKHFKEVLELVKSGKKQYAFIEFMGCHGG
                     CIFGGGQPITKAKDFEKIDVLKERAKALYRIDQSGKIRRSHENPHVLKLYEEFLGHPG
                     SHLAHKLLHTTYSKKDVY"
     gene            538..1431
                     /locus_tag="PLQ55_04050"
     CDS             538..1431
                     /locus_tag="PLQ55_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29135.1"
                     /translation="MKKKIATLLLTSLLLGCQKQTVKLSLLVPAGSPLLTVAGVTSEL
                     EKEVTVGPDLIPAAFTKGEKDLIAAPIIIGAKLFNAGKSNYQLAAMLGWGNLHILSRT
                     LINTIDDLSGKSGLAFAEHSTPGIMFKLATSDVSDINVNYVKAVSDVGGPFIQKQYDY
                     VLISEPVLSKIRSNINDETYTFSLQNVAKLPKIAQFGLFSNPATKAKDVNKFLVKLKN
                     NVEQLNTDPESYVDSIIDSDDYFKGLGRDVLIDAIPRLDIEYKSALEAKADLTLFFDY
                     LISVDTNLIGATSLDESFFRS"
     gene            1409..2173
                     /locus_tag="PLQ55_04055"
     CDS             1409..2173
                     /locus_tag="PLQ55_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29136.1"
                     /translation="MKASSVPKKKPTAQIIASSLGFLTIILLWIIISALHQNDIIFPG
                     IISTLKVFFSLFQRGPTYFILLNTIGGLILVLVLSFLTALILSLISLKFPTFKSYITP
                     FLALFKIIPVPALLIVFLVNFKQSFIPFLLTYIVLLPLVYDGLYGAFISINKDLIDEL
                     KITSKLNLLMIFKIYIPIVSKQVISTLLQALGLGLKVKVMSEFIAEAPNTIGYELSYG
                     RATLSMDIVFAWSLILIVIVVILDAVLVNYLGKQEA"
BASE COUNT          729 a          339 c          401 g          770 t
ORIGIN      
        1 aaccaattaa caagcccgtt tgcctcttac cctggcgctg gtgttatttt tggcgtaaca
       61 ggtggcgtta tggaagcagc attacgtact gttaaagcat tacttgaaaa agaagatgct
      121 tatttaatcg atattcgtga agtacgcggc tttgaaagta ttaaagaagc aaccttaaca
      181 attgatggta aagaacttaa ctttgcggtt gtgcacggcg ctaaacattt taaagaagtg
      241 cttgaactag taaaaagtgg taaaaagcaa tatgccttta tcgaatttat ggggtgccat
      301 ggcggctgta tctttggtgg aggtcaaccg attactaaag ctaaagactt tgaaaagatt
      361 gatgtattga aagagcgcgc taaagcactc taccgcatcg accaaagtgg aaaaatccgc
      421 cgttcacatg aaaatccaca tgtcttaaaa ttatatgaag aatttttagg ccatcctggc
      481 tcacatttag cgcataaact attacataca acatatagta aaaaggatgt gtattaaatg
      541 aaaaagaaaa ttgcgacttt gttattaact agcttacttt taggttgtca aaaacagact
      601 gttaaattat ctttattagt gcctgctggc agcccgttat taacagttgc aggtgtaaca
      661 agtgaattag aaaaagaagt gacagttgga cccgacttaa ttccggcagc gtttactaaa
      721 ggtgagaaag atttaatcgc tgcaccgatt attattggcg caaagttatt caacgctggt
      781 aaatcgaact atcaactcgc cgcaatgctc ggctggggta acttacatat tttaagtcgg
      841 acactaatta atactattga tgacttaagc gggaaaagtg gtttagcttt tgctgaacat
      901 tcaacaccag gcattatgtt taaattagcg acaagtgatg ttagtgatat taatgtaaat
      961 tatgttaaag cagttagtga tgtcggcgga ccatttatcc aaaaacaata tgattatgtt
     1021 ttaattagtg aaccagtttt aagtaagatt agaagtaata taaatgatga aacatataca
     1081 ttttccctgc aaaacgttgc taaattacct aaaatagcgc agtttggatt atttagtaat
     1141 cccgctacta aagcaaaaga tgtaaataaa tttttagtta aacttaaaaa caatgttgaa
     1201 caacttaaca ctgatccaga aagttatgtt gatagtatta ttgacagtga tgactacttt
     1261 aagggactcg gtcgagatgt tttaattgat gctattcccc gtttagatat tgaatacaaa
     1321 agtgcgcttg aagcaaaagc agatcttact ctcttttttg attatttaat tagtgttgac
     1381 actaatttaa taggagcgac gagtcttgat gaaagcttct tccgttccta agaaaaaacc
     1441 aacagcgcaa attattgcta gctcattagg ctttttgaca atcattttac tatggattat
     1501 tattagtgcg ctgcaccaaa atgatattat attccccgga attattagta ctttaaaagt
     1561 gtttttttct ttattccaaa gagggcctac atattttatt ttactaaaca ctattggggg
     1621 attaattctc gttttagttt tatcattttt aacggcatta attttaagtt taatatcgtt
     1681 aaaatttcca acttttaaaa gttatattac acccttttta gcactattta aaattatccc
     1741 tgttccagcg cttttaattg tgtttttagt taattttaaa caaagcttta ttccgttttt
     1801 actaacatat attgtgcttt taccacttgt ttatgatgga ttatatgggg cttttattag
     1861 cattaataaa gatttgattg atgaattaaa aattacttca aaacttaatt tattaatgat
     1921 ttttaaaata tacattccga ttgtaagcaa acaagttatt tcgactttat tacaagcctt
     1981 agggctagga cttaaagtaa aagttatgag cgaatttatc gctgaagcgc ctaatacgat
     2041 tggttacgaa ctctcttatg gacgggcaac tttatcgatg gatattgtat ttgcgtggtc
     2101 acttatttta atcgttatcg tcgttatttt agatgctgtt ttagtgaatt atttaggtaa
     2161 acaagaagct taaatttttt aatggacgtt aaaggaaaag gttaaattta catccttctt
     2221 gtcttcatta taatgttta
//
LOCUS       DAOPCD010000152         2208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_38842_length_2208_cov_3.148630, whole genome shotgun sequence.
ACCESSION   DAOPCD010000152 DAOPCD010000000
VERSION     DAOPCD010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38842_length_2208_cov_3.148630"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="PLQ55_04060"
     CDS             <1..1334
                     /locus_tag="PLQ55_04060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPK29137.1"
                     /translation="PAVKHLILVPPSVKGNLTKIKKGSFTVDEVIAIIETRDGVKELT
                     MLQKWPVRKKRPYVTKKPPKQAMVTGQRVIDTLFPIASGGIAAIPGPFGSGKTVVQHQ
                     LAKWADADLIVYIGCGERGNEMTDVLMEFPRLIDPKSGLSLMNRTVLIANTSNMPVAA
                     REASMYTGITIAEYFRDMGYRVAIMADSTSRWAEALREMSSRLEEMPGEEGYPAYLAS
                     RLSDFYERAGVVVTLGKKKRLGSITAIGAVSPPGGDTSEPVSQATLRIVKVFWGLSAS
                     LAYKRHFPAIDWLRSYSLYDDDIVKHHSASFPYWKDYVALLKELLQDEAALLEIVRLV
                     GIDSLSQKDRVKLHAAQMIREDYLHQNAFHAVDTYTSIEKQYGMLDLIVTWFKEANLA
                     LDEGVEYKQMENLAVNERISHIKYVEEKDFDKQVKALKKDVIAAFNNLKEE"
     gene            1339..>2208
                     /locus_tag="PLQ55_04065"
     CDS             1339..>2208
                     /locus_tag="PLQ55_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPK29138.1"
                     /translation="MAIKAFKTIKEVVGPLMVVENVSGVKFDELVRIIQSNGEERLGK
                     VLEIDNDKAVVQLFESSQGLKIDDAKAIFTGEGVTFGVSPHILGRKFDGLGRPIDGKG
                     HIIPFKRIDINGVPLNPVARDYPNEFIQTGISAIDGLNTLVRGQKLPIFSSSGLTHNR
                     LAAQIAKQATVLGRDEKFAVVFAAIGITFEEANFFSEAFEESDALDRTVMFFNLANDP
                     AIERIATPRMAITAAEYLAYELGMHVLVILTDITNYAEALREISAARKEVPGRRGYPG
                     YMYTDLASLYERAG"
BASE COUNT          669 a          401 c          516 g          622 t
ORIGIN      
        1 caccagcggt gaaacattta atcttagtgc cgcctagtgt taaaggtaac cttacgaaaa
       61 ttaaaaaagg ttcatttact gttgacgaag ttatcgccat tattgaaaca cgagatggtg
      121 ttaaagagtt aacaatgtta caaaaatggc cggtgcgaaa gaaaagacca tatgttacta
      181 aaaaaccgcc gaaacaagca atggttacgg gccaaagagt aattgatacc cttttcccta
      241 tcgctagcgg gggtattgct gccattcctg gaccatttgg tagtggaaag acagtcgttc
      301 aacatcaact cgccaaatgg gccgatgcgg atttaattgt ttatattgga tgtggggaac
      361 gaggtaacga gatgacggat gtattaatgg aattcccacg tctaattgac ccgaaatcag
      421 ggttatcgct aatgaatcga acagtcttaa ttgctaatac gtcaaacatg ccagttgccg
      481 cccgtgaagc aagtatgtat accggtatta caattgcgga atatttccgt gatatgggtt
      541 accgtgtggc gattatggcc gactctactt ctaggtgggc agaagcactg cgagaaatga
      601 gttcacgcct tgaagaaatg ccaggcgaag aaggctatcc cgcttactta gcaagtcgct
      661 taagtgactt ctatgaaaga gctggtgttg ttgtgacttt aggtaaaaag aaacgtttag
      721 gttcgattac cgcaattggc gcagtttcgc cgccaggcgg tgacactagt gaaccggttt
      781 cccaagcaac tttacggatt gttaaagtgt tctgggggtt aagcgctagt ctagcgtata
      841 agcggcattt cccggccatc gactggttac ggagttattc tttgtatgat gacgatattg
      901 ttaagcatca tagtgcctct ttcccgtatt ggaaagatta tgtcgctctt ttaaaagaat
      961 tattacaaga cgaagcagcg cttctagaaa ttgttcgtct tgtgggtatt gattcactta
     1021 gtcaaaaaga tcgagtcaaa ctccacgctg cgcagatgat tagagaagac tatttacacc
     1081 aaaacgcttt ccacgcggta gatacttata cttccattga aaagcaatac gggatgcttg
     1141 atttaattgt tacttggttt aaagaagcaa accttgcact tgacgaaggc gttgaataca
     1201 agcaaatgga aaaccttgcg gttaatgaac gcataagtca tataaaatat gtcgaagaaa
     1261 aagattttga taaacaagtt aaagcgctta aaaaagatgt tattgcggca tttaacaact
     1321 tgaaggagga ataaatatat ggcaattaaa gcatttaaaa caattaaaga agttgttggc
     1381 cccctgatgg ttgtcgaaaa tgtcagcggt gttaaatttg atgaacttgt gcgtattatt
     1441 caaagtaacg gtgaagagcg gcttggtaaa gttttagaaa ttgataatga taaagccgtt
     1501 gtccaacttt ttgaaagtag tcaaggatta aaaattgatg acgcgaaagc tatctttact
     1561 ggtgaaggtg ttacttttgg ggtatcacca cacattttag gaagaaagtt tgatggtctt
     1621 ggtcggccaa ttgatggcaa agggcacatt attcctttta aacgcattga tattaatggt
     1681 gtaccgctta acccagtagc gcgcgattat ccaaacgaat ttattcaaac gggcatcagt
     1741 gccatagatg ggttaaatac tcttgttcgc ggacaaaagt taccaatttt ctctagttca
     1801 ggtttaacac ataacaggct cgctgcgcaa attgctaagc aagcgacagt gttaggacgc
     1861 gacgaaaaat ttgcggttgt atttgccgca attggcatta cttttgaaga agcaaacttc
     1921 tttagtgaag catttgaaga aagtgatgcg cttgaccgga ctgtaatgtt ctttaattta
     1981 gcaaatgacc cagcgattga gcggattgca acaccacgta tggcgattac cgcggcagaa
     2041 tatcttgctt atgaacttgg gatgcatgtc ttagttattt taacggatat tactaactac
     2101 gctgaagctc tgcgtgaaat cagtgctgct cgtaaagaag taccaggtcg aagaggatat
     2161 cctggctaca tgtataccga cttagcatcg ctctacgagc gcgcgggt
//
LOCUS       DAOPCD010000153         2207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_38856_length_2207_cov_4.243030, whole genome shotgun sequence.
ACCESSION   DAOPCD010000153 DAOPCD010000000
VERSION     DAOPCD010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38856_length_2207_cov_4.243030"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PLQ55_04070"
     CDS             complement(<1..306)
                     /locus_tag="PLQ55_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29139.1"
                     /translation="MTEKEKMVEVVEETPAETKTPQAFVNEEISDDATNEQLELPALE
                     TMRNTFSKMYRKFRIFSYVSTAIVIAVIVVSYVWLLPINQWAGIVPIILVLVASLVYS
                     "
     gene            complement(315..956)
                     /locus_tag="PLQ55_04075"
     CDS             complement(315..956)
                     /locus_tag="PLQ55_04075"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK29140.1"
                     /translation="MMILNYTNKTNQELERLIKSNDPAAMHELARRLVQGEGIAEDNK
                     RGIELARQSEALGYAPADTVIGYCYFYGKGVEEDKQKGFAYFHSGAVKGHDAAQYNMG
                     IAYERGTVIPQDYALALEWYQRAADQKFAKAFYKLGYFHEHGMGVTKDVTKAFSYYEK
                     SANLGYVKAEVEVGKAHLSGRGLLRDLTKAREYLERAAAKGNRIAARLLRDIE"
     gene            complement(1001..1621)
                     /locus_tag="PLQ55_04080"
     CDS             complement(1001..1621)
                     /locus_tag="PLQ55_04080"
                     /inference="COORDINATES: protein motif:HMM:NF021062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HPK29141.1"
                     /translation="MKETTKKRRTLKPNRFFGRILTVRGITEMAIFIALALVFDLEFL
                     KFRLGEAQSFSITMLPLMIIALRFPLVDAFIGIGGIYGVITMLWDGYGLITYPLDYLL
                     AYGFLSIISLFQALVYGKHKHEIINYLYLALAVPAGVSGRIFFSTISGVVVYETPFVG
                     SLLYNLPAIGVSGIICGVLLMMLFPTLLRFRPQPFLQNEVEESKQQ"
     regulatory      complement(1662..1766)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1806..>2207)
                     /locus_tag="PLQ55_04085"
     CDS             complement(1806..>2207)
                     /locus_tag="PLQ55_04085"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPK29142.1"
                     /translation="SLRNIFIEMADDVGGPVRTDGDLTYLAKQGVFLINTILTVERSK
                     PLSHNIPEYKTLIVKVLEELNKRPQPIVFILWGSYARSFKKYINGPNKLVIESNHPSP
                     LSANRGGFFGTRPFSRANNFLAKHGSNVINW"
BASE COUNT          681 a          471 c          352 g          703 t
ORIGIN      
        1 tgaatacact aaagaagcaa caagtactaa aataattgga acaataccag cccactgatt
       61 aattggtagt aaccacacat aagaaacaac tatcacagca ataacaattg cagttgacac
      121 ataactaaaa atacggaact ttcgatacat tttcgaaaaa gtattccgca ttgtttctaa
      181 tgctggtaat tctaattgtt cgttagtagc gtcatcacta atttcttcat taacaaatgc
      241 ttgtggagtt ttagtttctg caggagtttc ctccactacc tccaccatct tttctttttc
      301 tgtcatataa ttctctattc tatgtcacgt aataagcgcg cagcaatacg gtttcctttc
      361 gcggcagcac gctctaaata ttcacgggct ttcgttagat ctcgtagtag tcctcggcct
      421 gataaatgag ctttaccaac ttccacttca gccttaacat aaccgagatt tgcagacttt
      481 tcgtagtaac taaatgcttt tgtcacatct tttgttacac ccatcccgtg ttcatggaag
      541 taacctagtt tgtagaaagc tttggcaaat ttttgatcag ccgctctttg ataccattca
      601 agcgctaaag cgtaatcttg cggaataaca gtcccgcgct cgtaagctat ccccatatta
      661 tattgagcag cgtcgtgacc tttaactgct ccgctatgga agtaagcaaa tcctttttgt
      721 ttatcttctt caacaccttt gccataaaaa taacaatacc caatcacagt gtccgctggc
      781 gcatagccaa gggcttctga ttggcgcgca agttcaatgc cgcgcttatt gtcttctgca
      841 ataccttcac cttgcactaa acgcctagca agttcatgca ttgcagcggg atcattactt
      901 ttaataagcc gttcaagttc ttgatttgtc ttatttgtat aatttaaaat catcataact
      961 tcttccccct tttagtataa catactaaac gcggttgatg ttattgttgt ttgctttctt
     1021 ctacttcgtt ttgtaggaat ggttgtggtc taaatcttag taatgtcggg aaaagcatca
     1081 ttaaaagaac gccacaaata attccagaca caccaattgc aggtaaatta tataagagtg
     1141 aaccgacaaa tggtgtttca tagaccacta caccactgat agtagaaaag aaaattcgcc
     1201 cacttacacc cgcaggtact gcaagagcaa gatataaata attaattatt tcgtgtttat
     1261 gtttgccata cactaaagct tggaataaag aaattataga aagaaaccca tacgctaata
     1321 agtaatctaa aggataagta attaaaccgt atccatccca taacatcgtg ataacaccat
     1381 aaattccgcc tatcccaata aaggcgtcaa caagggggaa acgcaaagca ataatcatca
     1441 gtggtaacat cgtaatacta aatgattgtg cttcaccaag ccgaaatttt aaaaattcta
     1501 aatcaaacac taatgctaaa gcaataaata ttgccatttc cgtaatacca cgcactgtta
     1561 aaatacgacc aaaaaatctg tttggtttta atgttcttct tttttttgtt gtttctttca
     1621 taattagtct cctttaaaca aaaagtctcc acgctgacgt gaagactctt atgttacatc
     1681 cctacgccag cattatctgt atcaggtgcg gtcgaacgtt cattgttcct ctcagccagg
     1741 cacacctagc tcccgtgagt tcattatatc aaatcgcaaa ctttgtagtg tttttttgtc
     1801 ttatcttacc aattaattac atttgacccg tgttttgcaa ggaaattatt agcacgcgag
     1861 aaaggtcgtg ttccaaaaaa gccacctcta ttcgcggata atggcgaagg gtgatttgac
     1921 tcaatcacta atttattagg accatttata tatttcttaa atgaccttgc ataactcccc
     1981 cataagataa agacaatcgg ttggggtctc ttatttaatt cttcaagtac tttaacaatt
     2041 aatgttttat attccggaat gttatgtgaa agtggtttac ttctttcaac agttaaaatg
     2101 gtattaataa gaaatacgcc ttgtttagct aaataagtta aatctccgtc cgtgcgaact
     2161 ggtccaccca catcatcagc catttcaata aatatatttc ttaagct
//
LOCUS       DAOPCD010000154         2194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_39168_length_2194_cov_4.199158, whole genome shotgun sequence.
ACCESSION   DAOPCD010000154 DAOPCD010000000
VERSION     DAOPCD010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39168_length_2194_cov_4.199158"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1734
                     /locus_tag="PLQ55_04090"
     CDS             <1..1734
                     /locus_tag="PLQ55_04090"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPK29143.1"
                     /translation="SYNVADRFVKTLRRDKHFVVDIKTKTCNLTDEGNKLAERMFGVN
                     NIYDPQYSDLVHRIHNALKANYVMSRDVEYMVDANREIQLIDQFTGRIMPGREYSDGL
                     HQAIQAKENVTIKEETVTMATITYQNFFRLYTKLSGMTGTAKTEEEEFRKIYNMRVIQ
                     IPTNRPVIREDAVDRVYARKEDKLNALVEEVKERHAKGQPILIGTSSVESSEEVSELL
                     TANGLKHEVLNAKNHAREADIVALAGNKGQITIATNMAGRGTDIKINDEVKALGGLAV
                     LGTERHEARRIDNQLRGRSGRQGDPGFSRFYVSFDDLLMRRFSSDMMLKLVEGMGNEA
                     LENKMFTNAITSAQKRIEGQNFDIRKSLLDYDNVLAKQRSIVYKKRDSIIYAGDIKDL
                     VQEQFTLAGLYLAKKAVPETGKDTLVNGVMLKQIMEPNYLPEGSINPSAYDESPPEEI
                     GEDLMLLMYRRYQLRRREWSEELADRVEREITLRAIDRNWTRHIDTMAHLREGIHLRS
                     YANTNPLQDYVNEGYKLFGEAMDIIAVETVLNLLNAKINVAPPVEEGQEGTEENKETA
                     EKENIKKEDKK"
     gene            1799..>2194
                     /locus_tag="PLQ55_04095"
     CDS             1799..>2194
                     /locus_tag="PLQ55_04095"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HPK29144.1"
                     /translation="MTLFDVASLKETIDKLTIEINNPDFWNNQNKAKTIIKKLNAAKE
                     KINIYSLVETTVESAFELLKDDTFAEDSEYLSDLAFEIDNAQKEGELLINLLLFSGEY
                     DENDATVSIHAGAGGTESHDWADMLYHMYA"
BASE COUNT          776 a          371 c          468 g          579 t
ORIGIN      
        1 agttataatg tcgccgatcg tttcgttaaa acattacgca gagataagca ctttgtcgtt
       61 gatattaaaa caaaaacatg taacttaacg gacgaaggta ataaactcgc ggaaagaatg
      121 tttggtgtta ataatattta tgacccacaa tatagtgact tagtgcaccg gattcataat
      181 gcgcttaaag caaattatgt catgagtcgt gatgttgaat atatggttga cgcaaatcgt
      241 gaaattcaac ttattgacca atttactgga cggattatgc ccggtcgtga atatagtgat
      301 gggttgcatc aagcaattca agcgaaagaa aatgtcacga ttaaagaaga aacagtgacg
      361 atggcgacga ttacctatca aaacttcttc cgcctttaca ctaaactaag cgggatgaca
      421 ggtactgcta aaaccgaaga agaagaattc cgcaaaattt acaatatgcg tgttattcaa
      481 atcccgacga atcgccctgt tattcgtgaa gacgctgtcg atcgagtata tgcacgtaaa
      541 gaagataaac tcaatgcact tgtcgaagaa gttaaagaac gtcacgctaa aggacaacca
      601 attcttattg ggacaagcag cgttgaatca agtgaagaag ttagtgaact tttaacagca
      661 aacggactca aacacgaggt acttaacgct aaaaaccacg cgcgtgaagc agacattgtt
      721 gcgcttgcgg gtaataaagg ccaaattaca attgcaacga acatggctgg tcgtggtact
      781 gacattaaaa ttaatgatga agttaaagca cttggcggcc ttgcggtatt agggacagag
      841 cgacatgagg ctcgccgtat tgataaccaa ttacgcggcc ggagtggacg gcaaggtgac
      901 cctgggtttt cacgcttcta tgtctcattt gatgacttac tcatgcgtcg tttctctagt
      961 gatatgatgc ttaaactcgt cgaagggatg ggaaacgaag cactagaaaa taaaatgttc
     1021 actaacgcta ttactagtgc ccaaaaacga attgaaggac aaaactttga tattcgtaaa
     1081 agtttattag attatgacaa tgttttagca aaacaacgct ctatcgttta taagaaacgt
     1141 gacagcataa tatacgctgg tgatattaaa gacttagtgc aagaacagtt tactcttgcg
     1201 ggtctttatt tagcaaaaaa agctgttcca gaaactggta aagatacgct tgttaatggg
     1261 gtaatgttaa aacaaattat ggaaccaaac tatttaccag aaggttcgat taacccttca
     1321 gcgtatgacg aatcaccacc agaagaaatt ggtgaagact taatgctttt aatgtatcgt
     1381 cgttatcaac ttagaagacg tgaatggagt gaagaacttg ccgaccgcgt tgaacgtgaa
     1441 attactttac gggcaattga ccgcaactgg acacggcata ttgacacaat ggcgcactta
     1501 cgcgaaggta ttcacttacg tagttacgca aatactaacc cacttcaaga ctatgttaat
     1561 gaaggttata agttattcgg tgaagcgatg gatattattg ctgttgaaac agttttaaat
     1621 ttattaaacg cgaagattaa tgttgcccca cctgttgaag aaggtcaaga aggcactgaa
     1681 gaaaataaag aaacagccga aaaggaaaat ataaaaaagg aagataaaaa ataaacgatg
     1741 gagtacaaag ttgccttaag taaagctaat gaacttaaaa gattactagg taagcttagt
     1801 gactctcttt gatgtcgcta gtttaaaaga aacaattgat aaattaacga ttgaaataaa
     1861 taatcctgac ttttggaata atcaaaataa ggctaaaaca atcattaaaa aattaaatgc
     1921 tgcgaaagag aaaattaata tatattcact agttgaaaca acagtggaaa gtgcctttga
     1981 actactaaaa gacgacactt ttgccgagga tagtgagtat ttaagtgatt tagcatttga
     2041 aattgataat gcgcaaaaag aaggtgaatt acttataaat cttctgcttt tcagcgggga
     2101 atatgatgaa aatgatgcaa ccgtaagtat ccacgctggt gcaggcggga cggagtcgca
     2161 cgactgggcc gatatgctct atcatatgta tgca
//
LOCUS       DAOPCD010000155         2193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_39206_length_2193_cov_2.925631, whole genome shotgun sequence.
ACCESSION   DAOPCD010000155 DAOPCD010000000
VERSION     DAOPCD010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39206_length_2193_cov_2.925631"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..692
                     /gene="trmB"
                     /locus_tag="PLQ55_04100"
     CDS             72..692
                     /gene="trmB"
                     /locus_tag="PLQ55_04100"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPK29145.1"
                     /translation="MRVKFKPGAIDYLKSLAFHTHVDDFALSKIKDINKPLVLEIGVG
                     KGDYLLEMATNYPEHFFIGIELNASVLSVAAQKLADANLNNILILTGDVKYLLPKLSA
                     LSLNYIILNHSDPWPKKRHEKRRLTYSTLVNEYYKLLEEGGTLIFKTDNDDFADYSYI
                     SLSSVPFKELIFIKDYKGDYDFDAKTEYERKFLNKGVKIKMIIGKK"
     gene            701..1771
                     /locus_tag="PLQ55_04105"
     CDS             701..1771
                     /locus_tag="PLQ55_04105"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPK29146.1"
                     /translation="MLTKRELKLLEKVTQIDAIPGLEQELAKFVKGELEKYGFTIVTD
                     NLGSVFGVKKSKVTQAPRIMVAGHLDEVGFSVREITDKGFIRVDARGGINANTMLASR
                     VRLTLDDGTKLMGSVGATAPHYGGEGNTTIDKLDFDFGFISKEDAVNAGVRVGQMIVA
                     DGPFEVLNGGKRLLAKAFDNRYGVFMALEMARYFENVDLPYDLYVGATVQEEVGLRGA
                     TTIANVINPDLVIILDCSPARDNISSSEQGQLGEGILIRYLDRSMIAFPELLTFQEKS
                     AEALKVPYQYFHSPGGTDAGAIHLHAVGVLTLTHCVCARSIHTSASVVDTTDIKNAKK
                     VLIRMLRTLNRDKINKFKEARR"
     gene            1776..>2193
                     /locus_tag="PLQ55_04110"
     CDS             1776..>2193
                     /locus_tag="PLQ55_04110"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF014769.2"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-diphosphate and nicotinate from nicotinate
                     D-ribonucleotide and diphosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HPK29147.1"
                     /translation="MTEIEMKNKDRKFRLTNKTFKFPSAIKDHFYTANYFVKTMEIAE
                     KHLADTVVTMQFFQRRDNVMACGVDESLALLQEFARYPEKLKIEACHDGDIVNANEPV
                     LKITGLYKDFAYLESLIDGILTRRTTAATNSYEVLQA"
BASE COUNT          709 a          334 c          469 g          681 t
ORIGIN      
        1 atttatttag atgattttta tacacagatg gcaatttacg ctcgtgctat tcattactta
       61 ggtaagaagg tatgagggta aaatttaaac caggtgctat tgattactta aaatcattag
      121 cgttccatac tcacgttgat gatttcgcat tgtcaaaaat taaagatatt aataaaccgt
      181 tagtgctaga aattggtgtt ggtaagggcg attatctttt agaaatggct actaactatc
      241 ccgaacactt ttttattggt attgaattaa atgcaagcgt ccttagtgtt gccgcgcaaa
      301 aactcgcgga cgcaaactta aataatatcc taatcttaac aggtgatgtt aaatatttat
      361 taccaaaatt aagtgcactt tcacttaatt acattatttt aaatcatagt gatccgtggc
      421 caaaaaagcg ccatgaaaag cgccgtttaa cttactcgac actcgttaat gagtattata
      481 agcttttaga agaaggtggc acactaattt ttaaaacgga taatgatgac ttcgcggatt
      541 attcttacat ctcgttaagt agtgttccct ttaaagagct tatttttata aaagattata
      601 agggtgatta tgattttgat gctaaaacgg aatacgaacg caaattcctt aataaaggtg
      661 ttaaaattaa aatgattatt ggaaagaaat aggtgtttat atgttaacaa aacgtgaact
      721 caaattactt gaaaaagtaa cgcagatcga tgcaattcct ggcttagaac aagaattagc
      781 aaagtttgtc aaaggcgaat tagaaaaata cggttttaca atcgttacgg ataatctcgg
      841 gtcggttttt ggtgtaaaga aaagtaaagt aactcaggca ccgcgcatta tggttgctgg
      901 tcatttagat gaagttggtt ttagtgtccg tgaaattact gataaaggtt ttattcgtgt
      961 tgatgctagg ggcggtatta acgctaacac gatgttagcg agtcgcgtac gtcttacgct
     1021 tgatgatggc actaaattaa tgggaagcgt tggtgcgacc gcgccgcatt atggcggaga
     1081 aggtaataca acgattgata aacttgattt tgacttcggc tttattagta aggaagacgc
     1141 agttaatgct ggtgttcgtg ttggacagat gattgttgca gatggtcctt ttgaagtatt
     1201 aaatggtggc aagcgcttac tcgctaaagc ttttgataac cgctacgggg tatttatggc
     1261 gcttgaaatg gcacggtact ttgagaatgt tgatttacca tatgacttat atgtcggtgc
     1321 caccgtgcaa gaagaagtag gattacgcgg ggcaacaact attgccaatg taattaatcc
     1381 agatttagtg atcattttag attgttcgcc cgcgcgcgat aatattagta gcagtgaaca
     1441 aggacaactc ggcgaaggaa ttttaattcg ttatttagat cgctcgatga ttgcttttcc
     1501 ggaattatta acattccaag aaaaaagtgc tgaagcacta aaagttccct atcaatattt
     1561 tcatagtcca ggtgggactg atgcgggggc cattcattta cacgctgttg gtgttttaac
     1621 actgacgcac tgtgtttgtg cgcgctctat tcacacttcc gctagtgttg ttgatacgac
     1681 tgatattaaa aatgctaaaa aagtattaat tcgcatgtta agaacattaa atcgcgataa
     1741 gattaataaa tttaaagaag ctaggagata agaaaatgac agaaattgaa atgaaaaaca
     1801 aagatcggaa gtttcgttta acaaacaaaa cctttaaatt tccaagtgcg attaaagatc
     1861 atttttatac cgcgaattac tttgtaaaaa caatggaaat tgctgaaaaa catttagcgg
     1921 acacagtcgt gacgatgcaa ttttttcaac ggcgggataa tgtgatggcg tgtggtgtag
     1981 atgaatcact agctttacta caagaatttg cgcgttatcc tgaaaaatta aagattgaag
     2041 cgtgtcatga tggtgatatt gttaacgcga atgaaccagt gttaaaaatt acaggtttat
     2101 acaaagattt tgcgtattta gaaagtctta ttgatggcat tttaacgcgg agaacgacag
     2161 cagcaaccaa tagttatgaa gtgttgcaag ctg
//
LOCUS       DAOPCD010000156         2190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_39280_length_2190_cov_2.783138, whole genome shotgun sequence.
ACCESSION   DAOPCD010000156 DAOPCD010000000
VERSION     DAOPCD010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39280_length_2190_cov_2.783138"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1457)
                     /locus_tag="PLQ55_04115"
     CDS             complement(66..1457)
                     /locus_tag="PLQ55_04115"
                     /inference="COORDINATES: protein motif:HMM:NF040973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sau3AI family type II restriction endonuclease"
                     /protein_id="HPK29148.1"
                     /translation="MDDKNRYLNEREIIEITKNAMNKTFGDFGLDESYNLRNKGGLGG
                     FVEENIFGYKQNNDDNYDFINAEIELKVTPVKRNLNGTISAKERLVLSMINYKEEALV
                     TFKTSSFYKKNKRLLIWFYLYLLDVHPVNYEIINYYLLEFENSLHYKVIERDWNIIHK
                     KIVAGKAHEISESDTEFLAACTKGANSRVLVDQYNSDIQAKPRAYSFKTSYMTYLFRN
                     IIHDIAPYSPLVSEDEWMKNPLEEIYKEKMSIYSGMSQQQLARRFNIKSRAKQINFMI
                     AQRMLGISGNKKSTAEMDIAGIIFRTITIDKNGRPTEAMPFKAFEFTELINTPWEEST
                     VREEFVDLKLMIFVFKEVDNVITFERIHFWNAPNEVVDGAIKEMYEECATLVSNGDAF
                     YFNASGKVIDKFPKENRNSNGICHVRPHARVSADKFDLPIPDKVTGITAYTKQSFWFN
                     KDFIEKILKESLF"
     gene            1599..>2190
                     /gene="dcm"
                     /locus_tag="PLQ55_04120"
     CDS             1599..>2190
                     /gene="dcm"
                     /locus_tag="PLQ55_04120"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020002316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HPK29149.1"
                     /translation="MEKTVVELFAGVGGFRVGLNNITAIDETGKAIEHKTFDFVWANQ
                     WEPSTKAQHAFDCYNLRFPSKTENSNVDISKVDKNTIPNHTLLCGGFPCQDYSVARSL
                     SGAQGIEGKKGVLWWQINDILTVKRPPFVLLENVDRLIKSPAKQRGRDLGVMLRCFYD
                     LGYALEWRVINAADYGHPQRRRRVFIFAYHQSNSYYK"
BASE COUNT          684 a          398 c          326 g          782 t
ORIGIN      
        1 ataattgttt cgtatttatg tttttagttt ccataacata attataacgc taaaacagct
       61 tttctttaaa acaatgattc ttttaatatt ttttcgataa agtctttatt aaaccaaaaa
      121 ctttgctttg tataagccgt gataccagtt accttatcag gaattggtaa atcgaattta
      181 tcagcactga ctctcgcgtg aggtcgcaca tgacaaatgc catttgagtt tctgttttct
      241 tttgggaatt tatcaataac ttttccacta gcattgaaat aaaaggcgtc accattactt
      301 actaaagttg cacactcttc atacatctct ttaatggcgc cgtcaactac ttcatttggt
      361 gcattccaaa aatgaattcg ttcaaaagtt attacattat cgacttcttt aaagacaaaa
      421 atcattaatt ttaaatccac aaattcttca cgcaccgtcg attcttccca cggcgtatta
      481 ataagctcgg taaattcaaa agctttaaat ggcattgctt ctgtcggtct tccgttttta
      541 tcgatggtta ttgttctaaa tataatgccc gcaatgtcca tttccgccgt tgatttttta
      601 ttgcctgaaa tgccgagcat cctttgggca atcataaaat tgatttgttt agcgcgtgat
      661 ttaatattaa atctgcgtgc taattgttgt tggctcatcc cgctataaat tgacattttt
      721 tctttataga tttcttctaa tggattcttc atccattcat cttcagaaac aagcggggaa
      781 tagggagcga tgtcatgaat aatatttcta aataaataag tcatgtatga agttttaaaa
      841 ctatacgccc ttggctttgc ttggatatct gaattatatt gatctactaa cacccgacta
      901 ttagcacctt tcgtacatgc tgcaaggaat tctgtatctg attccgatat ttcgtgagct
      961 ttacccgcaa caatcttttt atgaatgata ttccaatctc tttcaattac cttatagtgc
     1021 aatgaatttt caaattctaa taaataataa ttgatgattt cgtaattcac aggatgaaca
     1081 tctaataaat atagataaaa ccaaataagc aatcgcttgt ttttcttgta aaatgaactc
     1141 gttttaaaag taactaatgc ttcttcctta taattaatca tacttaacac cagtcgttct
     1201 tttgccgata tagtaccatt taaattacgt ttaactggtg taactttcaa ttcaatttca
     1261 gcattaataa aatcatagtt atcatcatta ttttgtttat aaccaaaaat gttttcttca
     1321 acaaagccgc caagaccgcc tttatttcgt aaattataag attcgtctaa tccaaaatca
     1381 ccaaaagttt tattcattgc atttttggtt atctctataa tttctcgttc gtttaaatat
     1441 ctatttttat catccatttt caatttgaat tatagcaaac attataaaaa atataatatc
     1501 cggccttttg taattttaat aataaataat ctaaagttat ttattctaat attacttttt
     1561 acttgatcaa aagtaattgt tgtagtaaac tagagcatat ggaaaaaaca gttgtagaat
     1621 tatttgctgg tgtcggtggc ttccgtgttg gtcttaacaa tattaccgct attgatgaaa
     1681 cgggaaaagc aattgaacat aaaacatttg attttgtatg ggcaaatcag tgggaacctt
     1741 caacaaaagc gcaacacgct tttgattgtt ataatcttag attcccttct aaaacagaaa
     1801 attcaaatgt agatatttcg aaagtcgata aaaacactat ccctaatcac acacttcttt
     1861 gtggcggatt cccttgccaa gattattctg tcgctcgttc actcagtggc gcccaaggta
     1921 ttgaaggaaa aaagggtgta ctatggtggc aaattaatga tattctaaca gttaaacggc
     1981 caccttttgt cttacttgaa aatgttgacc gcctaattaa atctcctgcc aaacaacgcg
     2041 gaagagactt aggggtaatg ctacgctgct tttatgacct tggatatgct ttagaatggc
     2101 gtgtcattaa tgctgcagat tacggccacc cgcaaagaag acggcgcgta tttatttttg
     2161 cctatcacca aagtaatagc tattacaagc
//
LOCUS       DAOPCD010000157         2181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_39507_length_2181_cov_2.726717, whole genome shotgun sequence.
ACCESSION   DAOPCD010000157 DAOPCD010000000
VERSION     DAOPCD010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39507_length_2181_cov_2.726717"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(399..>2181)
                     /locus_tag="PLQ55_04125"
     CDS             complement(399..>2181)
                     /locus_tag="PLQ55_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29150.1"
                     /translation="SHQCRQSFYHLNQIIYNLKTPSSEKPPVSSEQPPVTSVTTEEPP
                     VSSEQPSEPPVSSEEPPSSEVEPGEPIKLGDAAAALADPNEIYLQVGEKVNLTYARIV
                     DNVPRIQFTVEDGVAYDALKLFVKFPGEVGKRYKVNAAFTAAAAFTGTINGERFDFIR
                     GENNLEFYYDELENEVSIAFVFGFNEEGLGLKANNITFGEGSISERVYDDPETTLAVD
                     GDISDWSNLKAFENTVGVHSNTAEVKHKSVDFYASLTSTGLYLAAEAYHDILIEESEH
                     WWQSTNFEIFIQGNNQYWVSARKVDGAFTKRDNVTEAAMVTTPLTGEGVANFRTVAEM
                     FIANANLPEAAVVGGEIRVGFAWKTDGDLLDNGEAAGGGLDCYWVPKGTWTDNADQTF
                     VTINGIFKQTQLTFEPTTMTIDGDLTDWEEKAAYTTNKIYIEGSDATNHKNVTFMAFL
                     NDEGLFLAALAHHDVLINNEEMWHSNTNFEFFLNGGNQYYVTAAGQVSYGKGIIVTQA
                     YKGEPYAEYESVAEFFIPRWALPEGDMHRVGFAWKTNGDTATGLGGSGGGPDAWWFEA
                     GHFPNNADEQYYVTAEGLFRTDPTPAP"
BASE COUNT          626 a          462 c          405 g          688 t
ORIGIN      
        1 gcattcttcg gtggttcttt ataaatgttt ttgtcttttg ttgttaagac aatattgcgg
       61 ttatctagcg ccttaacact aaaggaataa atactagcac cgacatctaa aaaagttact
      121 ttaagcaagt cattttctaa aacatataat ttcataaaat tatccttttc cattgtataa
      181 atacatttta attatacata aaataagtat gttttgcagt aataaatgct gattttgcca
      241 ctaaataata gaaaaggtcc tattaatgat ataactagga ccaaattctt aattacgagc
      301 attttgtaaa caaggtctcc tcgcacggtg tgtgcgagga gatagagata ataatgaaat
      361 tatctgagta ttatttatgt ctaaacttta ttgtttaatt atggagcagg tgttggatca
      421 gttctaaaga gaccttcagc ggtaacatag tattgttcgt cagcattatt tgggaagtgt
      481 ccagcttcaa accaccacgc atctggtcca ccaccactgc cacctaaacc agttgccgtg
      541 tcgccattag tcttccacgc aaagccaacg cggtgcatat caccttctgg gagtgcccag
      601 cgcggaataa agaattcggc cactgattca tattctgcat acggttcgcc tttgtatgct
      661 tgtgtcacaa taataccttt accatagctt acttgccctg ctgcggtaac ataatactga
      721 tttccaccat taaggaagaa ttcaaagttc gtattagagt gccacatttc ttcattatta
      781 attaagacat catggtgcgc taaagcggca aggaagagtc cttcatcatt taagaaagcc
      841 atgaatgtaa cattcttatg gtttgttgca tcactacctt caatataaat tttgttagta
      901 gtgtaagcag ctttttcttc ccaatcagtt aagtcaccat caattgtcat ggtcgttggt
      961 tcaaatgtta attgtgtttg cttgaaaatc ccgttaatcg tcacgaatgt ttggtcagca
     1021 ttatccgtcc aagtaccttt tggtacccag taacagtcaa gaccaccacc agcagcttca
     1081 ccgttatcta ataagtcacc atctgtcttc caagcaaaac caacacggat ttcaccacca
     1141 acaaccgcgg cttctggtaa gttagcgtta gcaataaaca tttcagcaac tgtacggaaa
     1201 ttagcgacac cttcacccgt taatggggtt gtcaccattg cggcttctgt aacattatcg
     1261 cgtttagtga atgcaccatc aactttacgc gcactaaccc agtattggtt attaccctga
     1321 ataaagattt caaagttagt gctttgccac cagtgttcac tttcttcaat gagaatatcg
     1381 tgatatgctt cggcggctaa gtataaccca gtgcttgtca agcttgcata gaagtcgaca
     1441 ctcttgtgct tgacttctgc ggtgttacta tggacaccaa cggtgttttc aaaagctttg
     1501 agattactcc aatcgctgat atcaccatca acagcaagcg ttgtttctgg atcatcataa
     1561 acgcgttcgg aaatactacc ttcgccaaaa gtaatattgt tggcttttag gcctaaacct
     1621 tcttcattaa agccaaagac aaaggcaatg ctaacttcat tttctagttc atcgtaataa
     1681 aattctaaat tgttttcacc acgaataaaa tcgaagcgtt cgccattaat tgtgccagta
     1741 aaggccgcag cagcagtaaa tgccgcatta accttatagc gtttacctac ttcgccaggg
     1801 aatttgacga ataatttaag tgcgtcataa gcgacaccat cttcaacagt aaattggatg
     1861 cgaggtacat tatcaacaat tcgagcatat gtgagattta ccttttcacc aacttgtaaa
     1921 taaatttcat taggatcggc aagagcagca gcagcgtccc ctaatttgat tggctcacct
     1981 ggttcaactt ctgagcttgg tggctcttca gagcttaccg gtggctcact tggctgttct
     2041 gaagatactg gtggctcttc agtcgtcaca cttgttacag gtggctgttc cgaacttact
     2101 ggcggttttt cacttgaagg tgttttcagg ttataaatta tttgatttag gtggtaaaaa
     2161 ctttgcctac attggtgtga c
//
LOCUS       DAOPCD010000158         2148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_40287_length_2148_cov_2.573817, whole genome shotgun sequence.
ACCESSION   DAOPCD010000158 DAOPCD010000000
VERSION     DAOPCD010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40287_length_2148_cov_2.573817"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLQ55_04130"
     CDS             complement(<1..202)
                     /locus_tag="PLQ55_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010517660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HPK29151.1"
                     /translation="MKTFWFIVGSQSLYGEETLKQVEKNAKKIAKGLKTKYPIVYKET
                     VKTHAEAMRLIKDANYDENCLGI"
     gene            complement(206..1510)
                     /locus_tag="PLQ55_04135"
     CDS             complement(206..1510)
                     /locus_tag="PLQ55_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPK29152.1"
                     /translation="MYLGIEFGSTRIKGIVLNDNFEVIASGSYIWQSTLKDGYWTYDL
                     NEVWVGMNAVIDEIAKTINVKEIKSLGVSAMMHGFLPFDKNGKLLTPFRTWKNTNTTV
                     AATELSKQFAFNIPLRWSIAHLYQAYLDKESYVKDIDTFTTLAGYVHLTLTGKNVLGI
                     GDASGVFPIDVKTKHYNQKMIQTFNDLTDMKLEKLLPGIVEVGEDAGFLTAKGVELLG
                     NKLTVGIPLAPPEGDAETGMVSTNALKPKTGNVSAGTSVFSMIVLEKALNKWYPEIDI
                     VQTPTGDAVAMVHANECTSRIDPWIELFGEAIMRVNGEVDRNHLFETLYEVSLEDTPL
                     GEFMKQKLVEAIIDLKPGMDILLNKEHVKVDYLLGHGGYFKSKSAGRKVMSETLGIPI
                     RLLETAGEGGPWGMAVLAAYRYYNKEQKIKFDKFIEKVFKGA"
     gene            complement(1523..>2148)
                     /locus_tag="PLQ55_04140"
     CDS             complement(1523..>2148)
                     /locus_tag="PLQ55_04140"
                     /inference="COORDINATES: protein motif:HMM:NF027687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 43 C-terminal
                     domain-containing protein"
                     /protein_id="HPK29153.1"
                     /translation="VSALSATNYNSFRPYGNKVTIAHQFTYLTDDISYKRGWAGIGHS
                     TAFKDEEQWFFASHYRGTYMDRHRFFLGIWKMHFVNDWPVIEPNRYTGEGSINLEQAD
                     ISGNYRIQILHQSAVNSMLTDDIISIVNRAEEITLTKQKAGEWYVVNGEYNGRYRLNN
                     NQLELELNGVLYKGVITPQFNYEFEKGVLGLSLINSDGTALWGNRII"
BASE COUNT          637 a          461 c          325 g          725 t
ORIGIN      
        1 tgataccaag gcaattttca tcataatttg cgtctttaat taagcgcatt gcttctgcat
       61 gtgtcttaac tgtctcctta tagacgatag gatatttcgt tttcagtcct tttgcaattt
      121 tctttgcatt tttttctact tgcttgaggg tttcttctcc atataaagat tgtgacccaa
      181 cgataaacca aaatgttttc ataaattacg ctcctttaaa tactttttct atgaatttat
      241 caaattttat tttttgctct ttattgtaat aacgatatgc tgctaagaca gccatccccc
      301 acgggccccc ttcaccagcg gtctctaata aacgaattgg aataccaagt gtttcactca
      361 tcactttcct acccgcgctc tttgatttaa agtatccgcc atgccctaat aaataatcaa
      421 ctttaacatg ctctttgttt agtaagatat ccatgcccgg ttttaagtca attattgctt
      481 caacaagttt ctgcttcata aattcaccaa gcggggtatc ttctaaagaa acttcataaa
      541 gtgtttcaaa aagatgatta cgatcaactt caccattaac tctcataatt gcttcaccga
      601 aaagttcaat ccatggatca atacgtgaag tacattcatt cgcatggacc attgcgactg
      661 catcgcctgt tggagtttgg acaatatcaa tttcgggata ccatttattc aacgcctttt
      721 ctaaaacaat cattgaaaaa acagaagtac ccgcggatac attacccgtt ttaggtttaa
      781 gggcgtttgt tgataccatc cccgtttccg catccccctc tggtggcgct aaagggatac
      841 cgactgttaa tttattccct aagagctcca caccttttgc tgttaaaaag ccagcgtctt
      901 ctcctacttc aacaatacca ggcaaaagct tttcaagttt catatctgtt aaatcgttaa
      961 aagtttgaat cattttttga ttatagtgct tagttttaac atcaatgggg aacaccccgc
     1021 ttgcgtcgcc aatccctaat acatttttac cggtaagcgt tagatggaca taacctgcta
     1081 atgtagtaaa agtatcaata tcttttacat aactttcttt atctaaataa gcttgataaa
     1141 gatgggcaat tgaccatcgt aacggaatat taaatgcaaa ttgctttgat aactccgtgg
     1201 ccgctactgt tgtgttcgtg tttttccatg tgcggaatgg tgttaataat tttccgtttt
     1261 tatcaaatgg taaaaaacca tgcatcattg ctgacacacc aagtgatttg atttctttta
     1321 cattaatcgt cttagcaatt tcgtcaatta ctgcattcat cccgacccaa acttcattaa
     1381 gatcataagt ccagtaccca tctttaagtg ttgattgcca aatgtaagat cctgaagcaa
     1441 tgacttcaaa attatcattt aagacaatcc ctttaattct tgttgagcca aattcaattc
     1501 ctaaatacac atttcttcct ttctaaataa tacggttgcc ccataatgct gttccatcac
     1561 tattaatcag tgataagccc agcactcctt tttcaaattc ataattaaat tgcggggtaa
     1621 taactccttt gtataacacc ccatttaatt caagttcaag ttgattgtta ttaaggcgat
     1681 aacgaccgtt atattctccg tttacaacat accattcacc tgctttttgc ttagttaatg
     1741 taatctcttc agcgcgatta acaatcgaaa taatgtcatc agtaagcatt gaattaaccg
     1801 cggattgatg caggatttga atgcgataat ttccgcttat atcagcttgt tctaaattaa
     1861 tactaccttc accagtataa cgattaggtt ctataacggg ccaatcatta acaaaatgca
     1921 tcttccaaat accaagaaag aaacgatgac gatccatata agtgccgcgg taatgcgatg
     1981 caaagaacca ctgctcttca tctttaaatg ccgttgagtg accaatacct gcccagccac
     2041 gtttataact aatatcatct gtaagatatg taaattgatg ggcaattgtc actttgttgc
     2101 cataaggtct aaatgaatta taatttgttg cactaagtgc actaacac
//
LOCUS       DAOPCD010000159         2148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_40288_length_2148_cov_2.564262, whole genome shotgun sequence.
ACCESSION   DAOPCD010000159 DAOPCD010000000
VERSION     DAOPCD010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40288_length_2148_cov_2.564262"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1135
                     /locus_tag="PLQ55_04145"
     CDS             170..1135
                     /locus_tag="PLQ55_04145"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPK29154.1"
                     /translation="MIRADDGTYYAYGTQDNALWGDYFGVRYTPILSSTNLVDWEYRD
                     QAFKPATMPTWSETSGAGLWAPDIVKIGNTYNLYYSLSAWGDPNPGIGVATGPSPLGP
                     FTDQGKIFDSNEIGVYNSIDQAVFVDTDNRVYMFWGSFSGIYAVELTADGLSVKGTIA
                     EANANKVLVAGVDNKRWDIGNYEGAYIIRERGYYYMFLSTGTCCEGHHSTYEVRVCRS
                     ESILGPYYDKAGNLMTYRQNLGTLVVRGNDDFAGVGHNSIVKDDAGESYIVYHGFDKF
                     EDPYYGTTNRRALLIDKLVWDKEYWPSVEGLGPSRGAERPYIAPR"
     gene            1164..>2148
                     /locus_tag="PLQ55_04150"
     CDS             1164..>2148
                     /locus_tag="PLQ55_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPK29155.1"
                     /translation="MKKKTIPLLLLALVLTGCGQTSEKPSQPGGGGEGSVEVSLPTFE
                     NVALTYGNPITGADGTAMRRLVREFNEEYQGQITVTESFMPEVDFYESLVATIPMKRS
                     FDIALIHSYRVPSFANKNLLFPLDNLTASTGVDITRENYIEDVYDAMIWDNKQYGVPL
                     DVHSIMLYYNKDLLAQHGCEVPTNRAELIAAAKKMPQTNAGGWGLPLSTTWPSEYIFT
                     TALYQNGGKEIDKNTLMPAFNNTAGEKALKAVADLIHIEKISPLNVNVDADLLMFQQG
                     KAMFHINGNWMLNSLVDSGINFGVTSLADMFVDTPTADSKKVASRSHTFVLP"
BASE COUNT          645 a          385 c          484 g          634 t
ORIGIN      
        1 agataggatt tactcttatt ttatgcacct tgctagtggg atgtactcct ccagtttcta
       61 gtgaaacact acctcccact agtgaagatg tagtaactag ccaaagtttg gaagaagtac
      121 ccacaactta tgaaaatcca gtgtgggaac ccgttttagc tgaccctgcg tgattcgcgc
      181 ggatgatggg acttattatg cctatggtac gcaagataat gcgttatggg gtgattactt
      241 tggtgttcgg tatacaccaa tcttaagtag caccaatctt gttgattggg aatatcgtga
      301 tcaagccttt aaacccgcaa caatgccaac atggagtgaa acttcaggtg cggggttatg
      361 ggcaccagat atcgttaaaa ttggtaatac atataactta tattattcgc tttcagcatg
      421 gggcgatcct aatcctggga ttggtgtagc aacagggcct tcacccttag gcccatttac
      481 tgaccaaggc aaaatctttg atagtaatga aattggtgtt tataattcaa ttgaccaagc
      541 agtatttgtt gatacagata atcgtgttta catgttttgg ggcagtttta gcggcattta
      601 cgctgtggaa ttaactgctg atggacttag cgtcaaaggt acaatcgctg aagcaaacgc
      661 taataaagta cttgtcgctg gtgttgataa caagcgatgg gacattggaa attacgaagg
      721 tgcttatatc attcgtgagc gcggttatta ctatatgttc ctttcaacgg ggacatgttg
      781 cgaaggtcac cactcaactt atgaagtgcg tgtgtgtcgc agtgaaagta tcttaggacc
      841 ttattacgac aaagctggga acttaatgac ttatcggcaa aatctaggca cgctagttgt
      901 gcgtggtaat gatgactttg ctggggttgg gcataactca attgtcaaag atgacgctgg
      961 tgaaagttat attgtttatc acggttttga taaatttgaa gatccttatt acgggactac
     1021 aaatcgccgt gcattattaa ttgataaatt agtgtgggac aaggaatatt ggccaagtgt
     1081 tgaaggctta ggaccttcac gtggggccga gagaccttat attgcaccac gctaacaata
     1141 tacatttagg aaaggtaaaa attatgaaaa agaaaacgat tccattatta ctacttgctc
     1201 tcgtattaac gggctgcgga caaacaagtg aaaaacctag ccaacccggt ggaggtggtg
     1261 aaggtagtgt tgaagtatca ttaccgacat ttgagaatgt cgccttaact tacggtaacc
     1321 caattacggg tgcagatgga acagcaatgc gtcgccttgt ccgtgaattt aatgaagaat
     1381 atcaaggaca aattacggtg actgaatcat ttatgccgga agtagatttc tatgaatcac
     1441 ttgttgcgac aattccaatg aaacgttcat ttgatattgc tttaattcat agttatcgtg
     1501 taccatcatt cgctaataaa aacttattat tcccgctcga taatttaaca gcatcgacgg
     1561 gagttgatat aacgcgtgaa aattatattg aagatgtgta tgatgcgatg atttgggaca
     1621 ataagcaata tggtgtcccg cttgatgtac atagcattat gctttattac aataaagatt
     1681 tacttgcgca acatggctgc gaagtcccga ctaatcgcgc agaattaatc gctgcggcga
     1741 aaaagatgcc acaaactaat gctggtggct ggggcttacc gctttcaaca acatggccaa
     1801 gtgaatatat ttttacaaca gctttatatc aaaatggcgg gaaagaaatt gataaaaata
     1861 ctttaatgcc agcctttaat aacactgctg gggaaaaagc actgaaagca gtggcggatt
     1921 taattcatat tgaaaagatt tcaccactta atgtgaatgt tgatgctgat ttactcatgt
     1981 tccaacaagg gaaagcaatg ttccacatta acgggaactg gatgcttaat agtcttgttg
     2041 attcaggaat taattttggt gttacttcct tagcggatat gtttgttgat acaccaactg
     2101 cggatagcaa aaaagttgca agccgtagtc acacctttgt tttaccac
//
LOCUS       DAOPCD010000160         2115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_41171_length_2115_cov_2.706796, whole genome shotgun sequence.
ACCESSION   DAOPCD010000160 DAOPCD010000000
VERSION     DAOPCD010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41171_length_2115_cov_2.706796"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="PLQ55_04155"
     CDS             <1..1123
                     /locus_tag="PLQ55_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29156.1"
                     /translation="HKPENRAKLSMILTGSLSLITAVLLIVFSAVNINPYNLVKHDEN
                     ITRVYFQVSENSDINPLSPTASPVTLLNYIELDGEALTFEKKEGAPVYETQVYKTMEL
                     DIEDEIEVTYNFYVYNLIGVYTGEEEVNYKVTADAPMQSATLSEALDIIVKNIDDKAV
                     ISFNAVEKEAITPHFGRIAWVSLVILAVTSFYLTIRFGLTRGITNFVESFVAASLVFL
                     FLMVTRLAIPPLATMGAFVAILAVSFLNVVIFTNIKDVQEETASKGLTHREHFELGLR
                     YSLSVIYITVLMFSLGFLVFTLIGPLAVSSVYLASAVAMVAALLVVVKYSTAFLSGSL
                     NVLDRLPKLERKQKTVTGKQRTSIRSNEPEEAIFIGIND"
     gene            1174..>2115
                     /locus_tag="PLQ55_04160"
     CDS             1174..>2115
                     /locus_tag="PLQ55_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPK29157.1"
                     /translation="MNKFLDKLLKYYDLSLRDYEKLTAEVTKENLPTAEAFFNFESFI
                     SRIEKAIASDENVVIYGDYDADGILATSILKYAFLKRGKDVFTMIPSRYKDGYGLNTN
                     VVEKFSKRAINLIITVDNGINQHNAIDLANELGIDVLVSDHHELSETLPNAIAILHPE
                     LSNLVRLNSCGAYMALVISYGMLGYYDDYLISLAAIATLADMMPLIDRNRTLVRLAIK
                     ATNDHQYFPLVKLNDSTFFDETSMSMKIAPKINALGRLSQNYESNVLVEYFTTTDVKR
                     HYDISEYIEKVYKKRKAMSRGEELSSEELKNPVIALIT"
BASE COUNT          660 a          366 c          418 g          671 t
ORIGIN      
        1 tcataaaccc gaaaatcgcg cgaagcttag catgatttta actggtagtt taagccttat
       61 tactgctgtt ttacttatcg tcttcagcgc tgttaatatt aacccataca acttagtgaa
      121 acacgacgaa aatattacgc gtgtttactt ccaagtcagt gaaaatagtg atattaaccc
      181 actttcacca accgcaagtc ctgttacttt acttaattat attgagttag acggagaagc
      241 cttaaccttt gaaaagaaag aaggtgcgcc agtttacgaa acgcaagttt ataaaacaat
      301 ggaacttgat attgaagacg aaattgaagt tacttacaat ttctatgttt ataacttaat
      361 tggtgtttac actggtgaag aagaagttaa ctataaagtg actgctgacg cgccaatgca
      421 aagtgcaacg ttaagtgaag cgcttgatat tattgttaaa aatattgacg ataaagccgt
      481 tatttccttt aacgctgtcg aaaaagaagc aattacaccg cactttggcc gcattgcttg
      541 ggttagttta gtaatccttg ctgttacttc tttctactta acaattcgct ttggacttac
      601 gcgcggtatt acaaactttg ttgaaagctt tgtcgctgct agtttggtgt tcctattctt
      661 aatggtaacg cgtctagcaa tcccgccact agcgacaatg ggagccttcg ttgctatctt
      721 agccgttagt ttcttaaatg ttgtgatctt tacaaacatc aaagatgtcc aagaagaaac
      781 cgctagtaaa gggcttacgc accgtgaaca cttcgaactt ggacttcgtt atagtttaag
      841 tgtcatttat attactgtct taatgtttag tcttgggttc ttagtcttta ccctgattgg
      901 tccgcttgct gttagcagtg tctaccttgc tagtgctgta gcgatggttg cggcactcct
      961 cgtcgttgtt aaatatagta cggccttctt aagcggttcg ttaaatgtcc ttgatcgctt
     1021 accaaaactt gaacgtaaac aaaaaacagt cactggtaaa caacggacaa gtattagaag
     1081 taatgaacca gaagaagcca tctttattgg cattaatgac taatattaat taaataaata
     1141 caaaaagtac tacccgtgtg gtagtatttt tttatgaata aattcctcga taaattatta
     1201 aagtattacg acttatcact aagagattac gaaaaattaa cggcagaagt cactaaagaa
     1261 aatttaccaa cggccgaagc ttttttcaac tttgaatctt ttattagtcg gattgaaaaa
     1321 gcgatcgcta gtgatgaaaa tgtcgttatt tatggtgatt atgatgctga cggcattctt
     1381 gctacgagta ttttaaaata tgcctttctt aaacgtggta aagacgtttt tacgatgatt
     1441 cccagccgtt acaaagacgg gtacgggtta aacactaatg tggtcgaaaa gtttagtaaa
     1501 cgcgctatta atttaattat taccgttgat aacggcatta atcagcataa cgcaattgat
     1561 ctcgctaatg agctcggtat tgatgtactt gtgagtgatc accatgagtt aagtgaaacg
     1621 ctcccgaatg ctattgcgat tttgcatcct gaactaagta atttggtacg tcttaatagt
     1681 tgtggcgcgt atatggcgct agttataagt tacgggatgc taggatatta tgatgattat
     1741 ttaattagtt tagcggcgat tgcaacactg gcggatatga tgccactaat agaccgtaat
     1801 cgtactcttg tccgtctagc gataaaggcc actaatgacc accaatattt cccgcttgtt
     1861 aaattaaatg acagtacctt ttttgatgaa acaagtatga gtatgaaaat agcgccaaaa
     1921 attaatgcat tagggcgctt aagtcaaaat tatgaaagta atgtgttagt tgaatacttt
     1981 acgacgactg atgttaaacg ccattatgat ataagtgagt acattgaaaa agtatataag
     2041 aagcgtaaag cgatgagtcg cggggaagaa ttaagcagtg aagagttaaa aaatccagta
     2101 attgctttaa ttact
//
LOCUS       DAOPCD010000161         2113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_41209_length_2113_cov_3.111273, whole genome shotgun sequence.
ACCESSION   DAOPCD010000161 DAOPCD010000000
VERSION     DAOPCD010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41209_length_2113_cov_3.111273"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..919
                     /locus_tag="PLQ55_04165"
     CDS             209..919
                     /locus_tag="PLQ55_04165"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HPK29158.1"
                     /translation="MVKQQNNLFLLNNLFNVRDLGEIDTKYGTKTRRYKYIRGTAKGT
                     MTEIEKEHFYNLGVRVVVDLRYTNEIEKTPSPLRGYKDIKYYHVDMMGEFWQMREQGY
                     KDMTDLYIDLLDDSQEKLLTIFKIFLKYPRDGIYFHCTAGKDRTGVVTMLLLDLVGVP
                     HSVIVENYSESYTNNLSRPGYKFIKPEWRRFILSEPEYMERTLDHLYTKYGGSRAYLS
                     HIGLRDDEINNLAATIVE"
     gene            989..1744
                     /locus_tag="PLQ55_04170"
     CDS             989..1744
                     /locus_tag="PLQ55_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29159.1"
                     /translation="MKTLANLLDNDTVTIVLFFAFLLLAVVIYLIVSKKEPSKEKMRP
                     EERALKMVEAPKTEVKTEVTPEKPVTEEVKTKKVEREAQKVKVKKEDTDDDQFAAIKY
                     SYSYTAQVSLAPEASRGRYLKIKQEILSYTSVNNTISWREERFTLSGKTIVKFKLIGN
                     VLRMYIALTLEELSDVNIKIDDVSRYKEHELTPSLLRISGNTGVKRALQAIDILMQKL
                     ELKKDPQYKLGASDIPAAQTIDELLEAGLIRKI"
     gene            1748..>2113
                     /locus_tag="PLQ55_04175"
     CDS             1748..>2113
                     /locus_tag="PLQ55_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPK29160.1"
                     /translation="MKKIALVLGGGGAKGSYQIGVLKALHETKILKRVRVASGTSIGA
                     IHTLLLMHDLSIEEMINVWKEFTNEAIYGRNKFKETWNISGFYNTKGILEKILPFTNE
                     EAFRNTKIELWFLKCLKSSP"
BASE COUNT          786 a          329 c          408 g          590 t
ORIGIN      
        1 atcgcctcaa aaagtagttt tacactagaa aattggctta agtccaaaag tcaggaatta
       61 caaccaaaaa cacctaaaaa tacgaaaaaa atgataacga taacaatttt ttagccaatt
      121 tagcgacttt tcttagtaaa taatgcgttt tctttactaa agaaagaagt agtgtaatgt
      181 gatatcatag tgtaagcagg aggtagttat ggtcaaacaa caaaataatt tatttctctt
      241 aaacaactta ttcaatgtgc gtgacttagg tgagattgat acaaagtatg gtactaagac
      301 acggcgttat aaatatatta gaggtactgc caaaggcaca atgactgaaa tcgaaaaaga
      361 gcacttttat aacttaggag taagagtagt agtggattta cgctatacga atgaaatcga
      421 aaaaacacca agtccgttac gaggttataa agatatcaag tattatcatg tcgatatgat
      481 gggcgaattt tggcaaatgc gtgaacaagg ttataaagat atgaccgact tatacattga
      541 tttgcttgat gactcgcaag agaaactgct aacgatcttt aaaatcttct taaagtatcc
      601 gcgggacggt atttatttcc actgtaccgc tggtaaagat cggaccggag ttgtcacaat
      661 gttactccta gacttagtgg gtgttccgca ttctgtaatc gttgaaaatt atagtgaatc
      721 gtacactaat aacttatcgc ggccaggcta taaatttatt aaaccagaat ggcgacgctt
      781 cattcttagt gaacctgaat acatggaacg aacattagat catttataca caaagtatgg
      841 tgggagcaga gcttacttat cgcatattgg cttacgtgat gatgaaatta ataatctcgc
      901 ggccacaatt gtcgaataac attgcaaaaa gcacctaaaa taagtacaat tattgtgtaa
      961 acaccgctat aagcaaaagg agatacatat gaaaacctta gcaaacttac tcgataatga
     1021 tacagtaacg attgtattat tttttgcttt cctactctta gcggtcgtta tttatcttat
     1081 tgtttccaaa aaagaaccaa gtaaagaaaa aatgcggcct gaagaaagag cgctaaaaat
     1141 ggttgaagcg cctaaaacag aagttaaaac agaagtaacg ccagaaaaac cggttactga
     1201 agaagttaaa acaaaaaaag tagaaagaga agcacaaaaa gttaaagtta aaaaagaaga
     1261 cactgatgac gatcaattcg ccgccattaa atatagttat tcttatactg cacaagttag
     1321 tttagcgcca gaagcaagtc gcggacgcta tttaaaaata aaacaagaaa ttctctctta
     1381 cactagtgtt aataatacta tttcatggcg tgaagaacgc tttacattaa gcgggaaaac
     1441 aattgttaaa tttaaattga ttggtaatgt tttacggatg tatattgcct taacgcttga
     1501 agaattaagt gatgtaaata ttaagattga tgatgttagt cgttataaag aacacgaatt
     1561 aacaccaagt ttattacgta tcagtggtaa tactggtgtc aagcgtgcgc tccaagcgat
     1621 tgatatttta atgcagaaac ttgaactaaa aaaagaccca caatataaac taggggcaag
     1681 tgatatacca gcagcgcaaa caatcgatga actcttagaa gctggactta ttcggaaaat
     1741 ttaatttatg aagaagatag ccttagtttt aggtggtggt ggcgcgaaag gctcctatca
     1801 aattggtgta ttaaaagcgc tgcacgagac taaaatatta aaacgcgtta gggtcgctag
     1861 cggtacctcg attggggcca tccacaccct tttattaatg catgatttaa gtattgaaga
     1921 gatgattaat gtgtggaaag aattcacaaa tgaagcgatt tatggtagaa acaaattcaa
     1981 agaaacatgg aatataagcg gcttttataa cactaaaggt atcttagaaa aaatattacc
     2041 gttcactaat gaagaagcat ttagaaatac aaagattgaa ctatggtttt tgaaatgtct
     2101 taagagcagc ccc
//
LOCUS       DAOPCD010000162         2108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_41342_length_2108_cov_3.731125, whole genome shotgun sequence.
ACCESSION   DAOPCD010000162 DAOPCD010000000
VERSION     DAOPCD010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41342_length_2108_cov_3.731125"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..598)
                     /locus_tag="PLQ55_04180"
     CDS             complement(113..598)
                     /locus_tag="PLQ55_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29161.1"
                     /translation="MLIPFVVSAYLMQVFFLIGLFAGESFAWANYAGLVFTLLTLVFG
                     VIATVKSVTGDTRDTRKETMTFKLLLIPYFVINFIIGFMALLGALVNFMVLPIIVAGV
                     ILMLVFNYFMVVVTSASNIRYLIKNLVVKKDPLTLLHIAFHFIFVTDVISSVILALKK
                     D"
     gene            complement(932..1978)
                     /locus_tag="PLQ55_04185"
     CDS             complement(932..1978)
                     /locus_tag="PLQ55_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29162.1"
                     /translation="MKAKSLLKLASLFSLSVILLSLSGCNNSNTNTVVSFLPLEGSCG
                     AYGTAYHHGINYFLNNNEELNFEYINYDDSKIEETLKNKNTKAIIGSFNDELNNSVTS
                     NNDIPIITSFYKPIDYEYNADKAFFPLIPSLTKEAEALGDLMLLDESITSVGVLFSDE
                     ECYEEMYITLSEKLGHNIFVGYELDAEHDAYSAMLDIVIADNHDALVLFTNIYDFNNL
                     IYKLAIKDNETAVYAPSYIERFFNVPPNYNEDKFSIYYPKFTNSLNINEEELNATFIA
                     SEYYEELNPTIKKRITDNFSFFVDGYLAVSILNSAINEIDSKLINTKELTKLLHKNEL
                     FKLEAENLVKYTKS"
BASE COUNT          782 a          329 c          254 g          743 t
ORIGIN      
        1 aaaatcatta aaacacttaa tcttgttaat tttaattatt catctatcca aatattaatg
       61 ctttaaagga caaatattta aataaaaata tatttttgaa ttaagtaagc atttaatctt
      121 ttttaagcgc taagataaca ctactaataa catctgtgac aaaaataaag tgaaaagcaa
      181 tatgtaataa agttagagga tcttttttta ctactaagtt tttaattaaa taacgaatat
      241 tactagcgct agtaacgaca accataaagt aattaaagac aagcattaaa ataacgcctg
      301 ccacaataat tggtaagacc ataaaattca ctaacgctcc aagcagtgcc ataaagccaa
      361 taataaaatt aatcacaaag taagggataa gtaataactt aaatgtcatc gtttcttttc
      421 ttgtgtcgcg cgtatctcct gtgacacttt taacggtggc tataacacca aacactagtg
      481 ttaataaagt aaagactagc cccgcgtaat ttgcccacgc aaagctttca ccggcaaata
      541 aaccaattaa aaagaacact tgcattaagt aagcactaac aacaaaagga attaacatat
      601 tacttacctc cagaaaacgt gttgatataa cgttttctat cttccatatt atgccataat
      661 ttatacattt acttgaatca ttaactaaaa gaaacagaat acgtcatata atcatcatat
      721 taaccaatat acacgaatga tattttctca ttctttaaca ccaaccggag acttttggaa
      781 catttaatgg acagatttct tcatttttgg tggacaaatt tgactttttt gcggactatt
      841 ttgctttttg gttttatgtc aaaataaaat gatatttttt ttgtcataaa ctctaaaatt
      901 ctattgtatt tataaatttg ggcaaaatct cttaagattt agtatattta acaagatttt
      961 ctgcttctag tttaaataat tcatttttat gtaataattt cgtcaattct tttgtattta
     1021 taagtttaga gtctatttcg ttaattgcac tatttaagat agatactgct agatagccat
     1081 ccacaaagaa actaaagtta tcagttattc ttttctttat tgttggattt agttcttcat
     1141 aatactctga agctataaat gtagcgttaa gttcttcttc attaatattc aaagaattag
     1201 tgaatttagg gtaataaata ctaaacttat cttcattata gttaggcggg acattaaaga
     1261 atctttcaat ataactagga gcgtatacag ctgtctcatt atctttaatc gctaacttat
     1321 aaattaagtt attaaagtca tatatattag taaataacac taatgcatca tgattatcag
     1381 caataacaat atctaacatt gcactataag cgtcatgttc tgcatctaac tcatatccca
     1441 caaagatgtt atgtcctaac ttttcactaa gggttatgta catctcttca taacattctt
     1501 catcactaaa aagaacacca acactagtaa tactttcgtc aagtagcatt aaatccccta
     1561 atgcctctgc ttcttttgtt aatgaaggaa taagcggaaa gaacgcttta tcagcgttat
     1621 attcataatc aataggttta taaaagctcg taataatagg tatgtcatta ttagaagtaa
     1681 cactattatt aagttcatca ttaaaactac caataatagc tttggtattt ttattcttaa
     1741 gtgtttcttc tattttagaa tcatcataat taatatattc aaaatttaat tcttcattat
     1801 tatttagaaa gtaattaatg ccgtgatggt aagctgtacc ataagccccg caagaccctt
     1861 ctaatggtaa aaaagacaca acagtatttg tattagaatt attacaacca gataaactta
     1921 aaagaataac tgaaagacta aataaactcg ctaactttaa taaacttttt gctttcatac
     1981 actgattaac tatattaagt gtcgcaaaag aaaagagaac tgacaaggaa aaactacttt
     2041 tttaatattt taataacaaa atgatacttt aaattagttt ctttattttt ttgattactt
     2101 aatagaac
//
LOCUS       DAOPCD010000163         2106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_41404_length_2106_cov_3.921014, whole genome shotgun sequence.
ACCESSION   DAOPCD010000163 DAOPCD010000000
VERSION     DAOPCD010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41404_length_2106_cov_3.921014"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="PLQ55_04190"
     CDS             <1..1159
                     /locus_tag="PLQ55_04190"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HPK29163.1"
                     /translation="IEDQKSSLVEERGKISTNMQILKNERMPETKAIIRERQSKLEEN
                     YDAFFINDVAEPFFQNLIAEGKNPLDINAEYRQAFSNALYLSNSVFNQVKKLRKEYVV
                     DYRLTYDVETLHNDDFQMELNDLLEVKLPEYEEKIADAYRKAVKQFKDDFISKLRNAI
                     ETVEDQIAELNEALSMSTFGEDSYQFTVKPSTVYRQYYDMIKDDLLLKVTDDDSEFFQ
                     KYDDVMKDLFAQIVLAGDGDKNAAVLANVEKFTDYRNYLDFDLIVRNKDGHEQRLSRV
                     LMKKSGGETQTPFYISVLASFAYLYRVNEPGELSNSARLIIFDEAFSKMDSGRIKESI
                     RLLRRFGLQAIISAPSDKVADISPLVDETLVVLRGKNHSTVQLFAREEDLT"
     gene            1179..2036
                     /locus_tag="PLQ55_04195"
     CDS             1179..2036
                     /locus_tag="PLQ55_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29164.1"
                     /translation="MEAKFVDGIRAYHMRLRRRYLVELFVTISVFLAFCIALIVLDLL
                     SDFNMREPLFVPLLASATSISLLLFILTYFPIRFKRLQKALNLEFFQRAKTVKLELIP
                     NPLGGTKFDYYTLFNEAGMNVKSGAIKGVAPYEFNKRLFHVVYVYDDEKYSAIIHLPS
                     IDSPYYIQIHNGNFAPPEAYADNLIVKIAYVSPYKLQYYASSSATNAKIYLRKENETR
                     FVKFIKIYQATYQYVTTFTDEYLKVEGLTSPGRLRLGEKYDNKHYLELLTRLRLLQAL
                     MSAMLERGK"
BASE COUNT          695 a          364 c          417 g          630 t
ORIGIN      
        1 tattgaagac caaaaatcaa gtttagtgga agagcgcggt aagatttcca caaacatgca
       61 aattttaaag aatgaaagaa tgccggaaac aaaagcaatc attagagaac ggcaaagtaa
      121 acttgaagaa aattatgatg ctttctttat taacgatgtc gccgaaccat tcttccaaaa
      181 cttaattgcc gaaggtaaga atccgcttga tattaatgct gaatatcggc aagcatttag
      241 caatgcgctc tacctctcaa atagtgtctt caatcaagtg aagaaactgc gtaaagagta
      301 tgttgttgat tatcgcttaa catatgacgt tgaaacttta cacaatgacg acttccaaat
      361 ggaattaaat gacttgttag aagttaaatt accagagtac gaagaaaaaa ttgcggatgc
      421 atatcgtaaa gcggttaaac aatttaaaga tgacttcatt tcgaaattgc ggaatgcaat
      481 tgaaacggtt gaagatcaaa ttgctgaact taacgaagca cttagtatgt ctacatttgg
      541 tgaagactcg tatcaattca cggttaaacc aagtacagta tatcggcaat attacgatat
      601 gattaaagac gatttattac ttaaagtaac ggatgacgat agtgagttct tccaaaaata
      661 tgacgatgtg atgaaagatt tattcgcgca aatcgtgtta gcgggagacg gtgataaaaa
      721 tgcggcagtg ctcgctaatg ttgaaaaatt caccgattac cgtaattatt tagactttga
      781 cttaattgtc cgcaataaag atggccacga acaacgctta tcacgcgtat taatgaagaa
      841 gtctggtggg gaaacacaaa ccccgttcta cattagtgta cttgctagtt ttgcttatct
      901 ctatcgcgtt aatgaaccag gtgaacttag caacagcgcc cgtcttatta tctttgacga
      961 agcgtttagt aaaatggata gtgggcggat taaagaatca attcgcttat tacgccgctt
     1021 tggcttacaa gcaattatta gtgccccaag tgataaagtg gctgatatta gccccttagt
     1081 cgatgaaaca cttgttgtat tacgcggtaa aaatcatagc acagtgcaat tatttgcgag
     1141 agaagaggac ttaacttaac cgcaaagaaa ggagaattat ggaagcaaag tttgttgacg
     1201 gaataagagc atatcacatg cgcttacgtc gccgctactt agtggaacta tttgttacca
     1261 ttagcgtttt tctcgccttt tgcatcgcct taattgtcct tgatctcttg agtgacttta
     1321 atatgcgtga accacttttt gttccgctat tagcgagtgc taccagtatt tccttactct
     1381 tatttattct tacttatttc ccaattcgct ttaaaaggct gcaaaaagcg cttaatttag
     1441 agtttttcca aagagcaaag acagtaaaac ttgaacttat tcccaatccg ttaggcggca
     1501 cgaagtttga ttattataca ctttttaatg aagctgggat gaatgttaaa agtggggcga
     1561 ttaaaggtgt tgcgccttac gaatttaata agcggctttt tcatgttgta tatgtgtatg
     1621 acgatgaaaa atatagtgcg attattcact tgccaagtat tgattcacct tattacatcc
     1681 aaatccataa cggtaatttt gctccacctg aagcttacgc tgataattta attgtaaaga
     1741 ttgcgtatgt ttctccctac aaactacaat actatgcctc aagtagtgcc acaaatgcta
     1801 aaatatattt acgaaaagaa aatgaaacgc gctttgttaa atttatcaaa atttatcagg
     1861 cgacttatca atatgtgaca accttcactg atgaatattt aaaagttgaa gggctaactt
     1921 caccgggtcg attacggcta ggtgaaaaat atgataataa acattacctt gagcttttaa
     1981 cgagattacg cttgttacaa gccttaatgt cagcgatgct tgaaaggggg aagtaaaagg
     2041 atggcaaaag agcttatcgc aatgatttta gcggtaaaga tactggaata gaccttgttg
     2101 ccgaaa
//
LOCUS       DAOPCD010000164         2100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_41578_length_2100_cov_3.282152, whole genome shotgun sequence.
ACCESSION   DAOPCD010000164 DAOPCD010000000
VERSION     DAOPCD010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41578_length_2100_cov_3.282152"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PLQ55_04200"
     CDS             <1..707
                     /locus_tag="PLQ55_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29165.1"
                     /translation="GEYKSYTINNINGKVKKVYYGAAYVSLDDVAAYLFAFGEAPANQ
                     MMAKNSTAMNQAISLFGKYGRVNNGYYSSDVTKYKYEPSLPHTDNGGPVEADMYNYRE
                     MDFGYTYTPWGYGIESDDPYNNGTKITRSTVRFVYTAYDNEGNEGAKYIPVEHRHVFL
                     TINHYNDFFEYLNYEGGWSESFGWMSAGNEYCAGMNSSKYGKGYYDFENPVPPTKYNI
                     PQVKTLTELQSIFNCL"
     gene            complement(711..989)
                     /locus_tag="PLQ55_04205"
     CDS             complement(711..989)
                     /locus_tag="PLQ55_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29166.1"
                     /translation="MVELFFGFLQLSFYIIVFTFIPVTLFVRVLSVFPSKNDLKHKIL
                     IIIDIFSLSYYYFISKENKYRKLYNTLLFIYLALSIFAFGFAIHMYIL"
     gene            complement(982..1131)
                     /locus_tag="PLQ55_04210"
     CDS             complement(982..1131)
                     /locus_tag="PLQ55_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29167.1"
                     /translation="MNIKRFLMLLINAFVLFVILFMLTRTLWTLLVFVMPVVSLFLPK
                     EKSNG"
     gene            complement(1121..>2100)
                     /locus_tag="PLQ55_04215"
     CDS             complement(1121..>2100)
                     /locus_tag="PLQ55_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPK29168.1"
                     /translation="RISSVLILLIALLLPFSRQKPLVLDHNDDPFVFHLQKESLKIAQ
                     FTDLHLAFGFDANDRKTLKLISNITAETKPDLIVLSGDQTLSITAPARHRQLIRHMES
                     LKTPWTFVFGNHEADYHKMERLLKVIFTMNTKYMYFKVGPLLENGGYGNFTINYLYED
                     NPFYNLYLLDSKAELKIPGSGRLFVYDYFSEAQVDWYRNKVMIDKINNIKSTVFAHIP
                     LIQYADALLIENKDKVKGIIGERISHQEVDTGFFNAMVESTVSEAFFAGHDHLNNFTF
                     EKDGIILGYGQISGYNGYGTLRRGARIIEIDESKMFKTYVVYEDLSYEY"
BASE COUNT          744 a          336 c          358 g          662 t
ORIGIN      
        1 atggtgaata taagtcatac acaattaaca atattaatgg caaagtaaag aaagtctatt
       61 acggggctgc ttatgtttcg cttgatgatg ttgcggctta tttatttgct tttggtgaag
      121 cacctgcgaa tcaaatgatg gccaaaaata gtactgcaat gaatcaagca atatccttat
      181 ttggtaaata tggacgcgtt aataacggtt attacagcag tgatgttact aaatataaat
      241 atgaaccttc tttaccgcac actgataatg gtggaccggt tgaagcggat atgtataatt
      301 atcgcgaaat ggattttggt tacacttata cgccgtgggg atatgggatt gaaagtgacg
      361 atccatataa taacggcact aaaattacac gtagcactgt acgctttgtt tatactgcat
      421 atgataacga ggggaacgaa ggcgctaaat atattccagt tgaacatcga catgtgttct
      481 taacaattaa tcactataat gacttctttg aatatttaaa ttatgaaggc ggatggagcg
      541 agtcatttgg ttggatgagc gccggtaatg aatattgcgc tggtatgaac tcgagtaaat
      601 acggtaaagg atattacgat tttgaaaatc ctgtgccacc aacaaaatac aatattcctc
      661 aagtaaaaac acttactgaa ttacagtcga ttttcaattg cttatagtct ttacaatatg
      721 tacatatgaa tagcaaagcc aaaggcaaag atgctcaagg ctaaatatat aaaaagcagt
      781 gtattataaa gttttcgata tttattttct tttgaaataa agtaataata acttaaagaa
      841 aagatgtcaa ttatgattag tattttgtgt tttagatcgt ttttgcttgg aaacactgac
      901 aaaacacgca cgaaaagtgt aacaggaata aaagtaaaga caatgatata aaaagatagt
      961 tgtaaaaacc caaagaataa ctcaaccatt acttttctcc tttggtaaaa atagtgaaac
     1021 aactggcatc acaaatacta agagcgtcca taatgttctt gttaacataa ataaaataac
     1081 aaagagaaca aaagcgttaa ttaaaagcat taagaatcgt ttaatattca tagcttaaat
     1141 cctcatatac aacatatgtc ttaaacattt tagactcatc aatttcgatg atgcgcgctc
     1201 cgcgtctaag tgtgccgtag ccattatagc cacttatttg cccgtatcct aaaataattc
     1261 catctttttc aaaagtgaaa ttattaagat gatcatgtcc agcaaagaag gcttcactaa
     1321 cagtactttc aaccattgca ttaaagaaac ctgtatcaac ttcctgatga ctaatacgct
     1381 cgccaataat gcctttaacc ttatctttat tttctattaa taatgcgtca gcgtattgta
     1441 ttaaagggat atgggcaaat actgtcgatt ttatgttatt tattttatca atcataactt
     1501 tattacgata ccaatcaacc tgtgcttcac taaagtaatc ataaacaaaa agtctacctg
     1561 aaccagggat ttttagttct gctttagaat caagtaagta cagattataa aaaggattat
     1621 cttcatataa gtaattaata gtaaagttac cataaccacc attttctaaa aggggaccaa
     1681 ctttaaaata catatacttt gtattcatag taaaaatcac ttttagtaac cgctccattt
     1741 tatgatagtc tgcttcatga ttaccaaata caaaagtcca cggtgtttta agtgactcca
     1801 tatgacgaat taattgtcgg tgtctagcgg gagcagtaat tgaaagtgtt tggtcgccag
     1861 ataaaacaat taagtctggt tttgtttccg cggtaatatt actaattaat tttaatgtct
     1921 tccgatcatt agcgtcaaat ccgaacgcga gatggaggtc ggtaaactgc gctattttta
     1981 aagattcttt ttgtaaatga aaaacaaaag gatcatcatt atgatctaaa acgagtggtt
     2041 tttgtcgcga aaaaggtaga agtagcgcta ttaaaagtat taaaacacta ctaatcctta
//
LOCUS       DAOPCD010000165         2070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_42341_length_2070_cov_3.200496, whole genome shotgun sequence.
ACCESSION   DAOPCD010000165 DAOPCD010000000
VERSION     DAOPCD010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42341_length_2070_cov_3.200496"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PLQ55_04220"
     CDS             complement(<1..882)
                     /locus_tag="PLQ55_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29169.1"
                     /translation="MKTFHFNDDYALINFDGINVNDALLILNSKSFEKVFRSFHRSLK
                     EKESVLLVSLGKLTATDLLEFYSLLFKYELSTVLRRMPKVTKDHVLPIYELTESFYDY
                     WRKYERFGLLNAFKEDGTQTKVDDLLKVSNDFNYQIITIYRTISQKLLGQTFNVYRQL
                     PAGISANMLYTYHSFDKSLKEYKLLNNIPVATKIVINPPFIINSKSNVRKGVFTPLTY
                     SYNPITHLKINKNNFIAFPLWVGPLLAYVYIHIDLLHHGIALSNLFEIADYQKVKDLK
                     PNLIYIYGVEDSLYDSKF"
     gene            986..>2070
                     /locus_tag="PLQ55_04225"
     CDS             986..>2070
                     /locus_tag="PLQ55_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPK29170.1"
                     /translation="MLFSPEIKESPTLLLGEKARQKKEKGEKVISLAVGEPDFPTPEY
                     IIEATKKALDEGYTKYSTPQGLLELRTLIAKEYKPYKAEEVIIFPGAKAAIHAALTSI
                     LEPGDEVIIVEPYYVSYPAMIKLAEPKAKIVKIALNKDFTFPLEKLEEKITKKTKLLI
                     LNYPHNPTGQILSFQETMKIVNLVFRNKIYLLSDEIYDKLNFSGKAFHSFAEFPLIKD
                     QLVVINGFSKTYAMTGFRLGYALANKELINKMNLVNQNINTNTATFIQKGAVAALTNK
                     PMFLIPYNNELKERALYLHEEINKLPPFSGVLPEGALYYFMNISKTKLTSTEFANKLL
                     EEHNLLVVPGIAFGEKFDKYVRLSLCV"
BASE COUNT          757 a          325 c          329 g          659 t
ORIGIN      
        1 aaatttagag tcatataacg aatcttcgac accataaata taaattaagt ttggttttaa
       61 gtctttgact ttttgataat ccgcgatttc aaaaagatta gataaagcga taccgtggtg
      121 taataaatca atatggatgt agacgtacgc tagaagtggt cccacccata acgggaaagc
      181 aataaagtta tttttattta tttttaagtg agtaattgga ttataagaat aagttaaagg
      241 tgtaaagact ccttttctca cattactctt tgaattaatg ataaagggtg ggttaataac
      301 gattttcgtc gctacaggga tattattaag cagtttatat tcttttaaac ttttatcaaa
      361 agagtgatag gtataaagca tatttgcgct tatccccgcc ggaagttgcc gatatacatt
      421 aaatgtttgt cctaataact tttgactaat agtcctgtat atcgtaataa tttgatagtt
      481 aaagtcatta cttactttta agagatcatc gactttagtt tgagtaccat cttccttaaa
      541 agcattaagt aacccaaatc tttcatactt ccgccagtag tcataaaagc tttcggttaa
      601 ttcatatatt ggtaaaacat gatctttcgt gactttaggc atccgtctta acacagtact
      661 aagttcatat ttaaaaagaa ggctatagaa ctctaataaa tcagtcgcgg ttagtttacc
      721 taaactaaca agtaaaacac tctctttttc ttttaaagag cggtgaaagc ttctaaatac
      781 tttttcaaaa cttttggagt ttaatattaa gagcgcatca ttaacattaa taccatcaaa
      841 gttaattagt gcatagtcat cattaaagtg aaaagtcttc atcaatattc cccttctctt
      901 aattatataa tgtaccataa gcgaccattt tttatcaaaa aatgagcaaa atatggtaaa
      961 attgataaga aaagaggtat tttttatgtt atttagtccg gaaataaaag aaagtccgac
     1021 acttttactt ggtgaaaaag cgcggcaaaa aaaagaaaaa ggtgaaaaag ttatttcact
     1081 agcggtaggc gaacctgatt ttcctacgcc tgaatatata attgaggcaa caaagaaagc
     1141 acttgatgaa ggatatacga aatattccac tccgcaaggg ttattagaat tacgaacttt
     1201 aattgctaaa gaatataaac cttacaaagc tgaagaagta attatctttc ccggcgctaa
     1261 agcggcaatt cacgcggcat taacgagtat tcttgaacca ggtgacgaag ttattatcgt
     1321 tgaaccttat tacgttagtt atcccgcaat gattaaacta gcggaaccta aagctaagat
     1381 cgttaaaata gcacttaata aagattttac tttcccacta gaaaagttag aagaaaagat
     1441 tactaaaaaa acaaaattat taattcttaa ttatccgcat aaccccactg gtcaaatctt
     1501 atcattccaa gaaacaatga aaattgttaa tttagtattt aggaataaga tatatttact
     1561 aagtgacgaa atatatgaca aacttaattt cagcggtaaa gccttccact cttttgcaga
     1621 attcccgctt attaaagacc aactagtggt catcaatggc tttagtaaaa catacgctat
     1681 gacagggttt agattaggct atgctctcgc taataaggaa ttaataaata agatgaattt
     1741 agtaaatcaa aatattaaca ctaataccgc aacctttatt caaaaaggcg cagtagcggc
     1801 actaactaat aaaccaatgt ttttaattcc gtataataac gaactaaaag aaagagcact
     1861 ttatttacac gaagaaataa ataaattacc accctttagt ggtgtattac ctgaaggtgc
     1921 tttatattac tttatgaata ttagtaaaac aaaactaaca agtacagagt tcgctaataa
     1981 attattagaa gaacataatc ttcttgtagt gccgggtatt gcctttggtg aaaagtttga
     2041 taaatatgta agacttagct tatgtgtcag
//
LOCUS       DAOPCD010000166         2068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_42396_length_2068_cov_4.263289, whole genome shotgun sequence.
ACCESSION   DAOPCD010000166 DAOPCD010000000
VERSION     DAOPCD010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42396_length_2068_cov_4.263289"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PLQ55_04230"
     CDS             <1..288
                     /locus_tag="PLQ55_04230"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HPK29171.1"
                     /translation="EPLQTIIASDYGLYGIDEILALSIVNVYGSIGSTNFGYVDKVKP
                     GIIGELDKKTNGKVVNTFLDDIVGAIAAAAASSIAHKYADADFDTELYKKQ"
     gene            complement(346..729)
                     /locus_tag="PLQ55_04235"
     CDS             complement(346..729)
                     /locus_tag="PLQ55_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HPK29172.1"
                     /translation="MDKTKRNRLIMWLVLSGVTLIAATLILVLRKDYSTRGFSDATFI
                     PGVVVLFLFLLRLVRDAGTFDLVSYSISRIFQGTKEKSLDGMKTASEYVEGKRQERLK
                     KDRYYLPYIIIAVAFIVAGVILAYI"
     gene            complement(885..1850)
                     /locus_tag="PLQ55_04240"
                     /pseudo
     CDS             complement(885..1850)
                     /locus_tag="PLQ55_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057816.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          573 a          400 c          369 g          726 t
ORIGIN      
        1 gaacctttac aaacgattat tgctagcgat tatgggcttt atggcattga tgaaatctta
       61 gcgttatcaa tcgttaatgt ctatggttca attggttcca ctaactttgg ttatgtcgat
      121 aaagtaaagc caggcattat tggtgaatta gataagaaaa caaatggtaa agtcgttaat
      181 acattccttg atgatatcgt tggcgctatt gcggctgcgg ccgctagttc gattgcgcat
      241 aaatacgctg atgcggactt tgatactgaa ctttataaaa agcaataatc acaaaacaaa
      301 acacgaaaaa agcccggttc aatcgggctt ttcattaact ccaagttaaa tgtacgctaa
      361 gattactcct gcgacaataa aagcaacagc tataatgata tatggtaagt aataacgatc
      421 ttttttaagt cgttcttggc gtttaccttc aacatattca ctagcggttt tcatgccatc
      481 aagtgacttt tctttagtac cttggaagat gcgcgagata ctgtaggaca ctaagtcaaa
      541 agtgccagca tctcttacta atcttaagag aaataagaat aagacaacaa ctcctggaat
      601 aaacgttgca tcgctaaatc cgcgagttga ataatccttg cgtaacacaa gaattaaagt
      661 cgcggcaatt aatgtaactc cgcttaaaac tagccacata attaagcgat tgcgttttgt
      721 tttatccatc gttatttttt cggcttgtcg gctttagctt tagcagtgct tttcgctttg
      781 ggcttcgctg cttttttggg ttcagccttt ggctccgcct tctttttagc ggctctagcc
      841 gcttcttttt tggcttctgc ttctgctttt ttatctaact ctttaatttc ttcgcgataa
      901 cgttccattt cctcactatt tgctaagaca aagtgacctg gtaagatttc ttgtagtgtt
      961 ggtttttctt cacgataatc gtgagcggta aacgggttat ataaaatccg ttcacgggtt
     1021 ttttcactat aaggatctgg ttttggaatg gcgcttagga gtgcttttgt atatggatgt
     1081 aatgggtgtt taaataactc atcactcgtt gttaattcaa caatattacc ataatacata
     1141 actgcaatcc ggtcacagaa atatttaaca acacttaagt cgtgagcgat aaagagaatc
     1201 gttaagccaa gttcatcacg taagtcgtta agtaagttaa taatttgtgc gcgaatcgaa
     1261 acgtcaagcg cactgactgg ttcgtcggca ataatgaatt caggattcat aatcagcgct
     1321 ctagcaatcc caatccgttg ccgttgacca ccactaaatt cgtgcgggta ccgcgaagca
     1381 tagtcgctaa ttaatccaac cttttctaag gattcgataa ctttcttcga gttttcagcg
     1441 cggtctttca tcccttgaat ttggagacct tcttgaataa tatcttcaac agtcatccga
     1501 gggttaagac tatcaattgg atcttggaaa atcatttgca tcttattcat gatacttgta
     1561 tcaacatgtt tcatatcata tttaatttgt gcaatctttt ctttttgtgt cgagacgata
     1621 tccgcaagtt ttgcctttaa gtcagcaata acttcgtttg ttgctgtttt aagttcacta
     1681 acacgcaagt cataagcttc ttttgcttta acggcaaata cttttgtctc tgccgcaagt
     1741 tcttctttga ctgttggtga aagattttta tcaccagctt taataactgc ttttaattct
     1801 aaaacacgtt tactatcttt atcatgttcc ttaacaaaag cttttttaat tatttcaaac
     1861 ttatttgttt cacgtttaag ttgaccttca tactttttat taatttccgc aacgataata
     1921 tcattatcat cttgactatc gtcactacgg tcacggagtt cttctttaag tgcttgtttt
     1981 aagtcacgaa ctttagtttt atagttatat tcatcagtgg caaatgtatt tttgcgttct
     2041 ctaattgtca ctaaaagggc agcaaact
//
LOCUS       DAOPCD010000167         2066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_42460_length_2066_cov_2.727996, whole genome shotgun sequence.
ACCESSION   DAOPCD010000167 DAOPCD010000000
VERSION     DAOPCD010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42460_length_2066_cov_2.727996"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /gene="obgE"
                     /locus_tag="PLQ55_04245"
     CDS             complement(<1..779)
                     /gene="obgE"
                     /locus_tag="PLQ55_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPK29173.1"
                     /translation="MFIDQAKITLKAGNGGNGMVSFRREKYVPKGGPDGGNGGRGGSI
                     YFVATSNISTLTDFRFGLTIKANNGGNGATKNQYGKSGEDLYVKVPIGTVVYLEPSRE
                     VIADFKLEGETMLIAKGGKGGRGNAAFKSSTNRVPRIAEHGVPGEELLVTLELKLLAD
                     IGFIGLPNAGKSTLLTVISEARPQIDNYPFTTLRPELGVVKANSGNRFVAADLPGIIK
                     DAHLGRGLGLRFLRHAQRCKVLAFIIDGENSEDYYKEYKLLR"
     gene            complement(783..1745)
                     /locus_tag="PLQ55_04250"
     CDS             complement(783..1745)
                     /locus_tag="PLQ55_04250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK29174.1"
                     /translation="MAKKLVTVVVPMYNEEPMVPHFLTKIAEIIKENDGYNFEVVAVN
                     DGSRDNTLALLYDFQKKYKFLRVVNLSRNFGHEAAVAAGLSVAKGDAIIPIDADLQDP
                     PELIKPMLELFEEGYDVVNARRASRKEDTWIKRYTAGKFYRVIDKLSGKVRIPQNVGH
                     YRLISRRVLDEVLKLPESNRVFRVEVPYVGFKTAEVAFVRPKREHGKTHYNMKSMIDL
                     AINAITITSTKPLNFVFKFAMWLGIIFGLSAVTQLVLFILEMTKVLVLPFLNHGVWLL
                     INVSLFLTTVILLALTIISIYLGKTFNETQKRPFYVIESVKENK"
     gene            complement(1834..>2066)
                     /gene="rpmA"
                     /locus_tag="PLQ55_04255"
     CDS             complement(1834..>2066)
                     /gene="rpmA"
                     /locus_tag="PLQ55_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPK29175.1"
                     /translation="TRNGRVSTGRRLGAKRADGQLAKAGAILYRQRGTRIYPGLNARR
                     GSDDTIYATVEGIVRFERFGKTRKRVSVYPVN"
BASE COUNT          615 a          464 c          355 g          632 t
ORIGIN      
        1 cgcagtaact tatattcttt atagtaatct tcactatttt caccgtcaat gatgaaagcg
       61 agcaccttac aacgctgcgc atgccgtaaa aaacgtaaac caagtccgcg tcctaaatgt
      121 gcatctttaa taatgcctgg taagtccgca gccacaaaac ggttaccgct attagctttt
      181 acaactccta gttctggtct aagtgttgta aaaggataat tatcaatttg tggtcgtgct
      241 tcactgatca cggttaaaag tgttgattta ccagcgttag gtaagccaat aaaaccaata
      301 tccgcaagta attttaattc aagtgtcaca agtagttctt caccaggtac accgtgctca
      361 gcaatacgag gcacgcgatt cgtgctactt ttaaaagcag cattcccgcg accacctttc
      421 ccacctttag cgattaacat tgtttcgccc tcaagtttaa agtcggcaat tacttcgcgg
      481 cttggttcaa gataaacaac agtgccgatt ggtaccttga catagagatc ttcaccactt
      541 ttaccgtact gatttttagt tgcgccatta ccgccgttat tagccttaat cgttaaacca
      601 aagcgaaaat cagtaagtgt tgaaatatta cttgtagcga caaagtaaat ggaaccgccg
      661 cgacccccat tcccgccatc tggcccccct tttggcacat acttttcacg gcgaaaagag
      721 accatcccat taccgccatt acccgcttta agtgttattt tagcttggtc gataaacatt
      781 ctttatttat tttcctttac tgattcaata acataaaatg ggcgtttttg cgtttcatta
      841 aatgttttgc caagataaat tgagattatt gtaagcgcta aaaggataac tgttgttaaa
      901 aataaactaa catttatcaa taaccacaca ccgtgattaa gaaaaggcag cactaatacc
      961 ttagtcattt ctaaaataaa tagcacaagt tgcgtcacgg cacttaaacc aaagataatc
     1021 cccagccaca ttgcaaactt aaagacaaag ttaagtggtt tagtactcgt aattgtaata
     1081 gcgttaattg ctaaatcaat cattgacttc atattgtaat gcgttttacc atgttctctt
     1141 ttgggtctaa caaacgctac ttccgctgtt ttaaagccaa cataaggaac ttctactcta
     1201 aagacgcgat tgctttccgg taattttaaa acttcatcaa gcacgcgtct tgatataagc
     1261 cgataatgac caacattttg cggaatccgt acttttcctg ataacttatc aattactctg
     1321 taaaatttac cagcagtgta acgcttaatc catgtatctt ctttacgcga agcacgacgg
     1381 gcattaacga catcatatcc ttcttcaaaa agttcaagca tcggtttaat aagctccggt
     1441 ggatcttgta aatcagcgtc aattggaata atagcgtcac ccttggcaac acttagtcct
     1501 gccgccactg ctgcttcgtg gccaaagtta cgacttaaat ttacgacacg taaaaattta
     1561 tattttttct gaaaatcata aagtaaggcc agcgtattat cacgactccc gtcgttaacc
     1621 gctactactt caaagttgta gccatcattt tctttaataa tttcggcgat ttttgttaaa
     1681 aaatggggaa ccatcggttc ttcgttatac attgggacga caaccgtcac taatttttta
     1741 gccatgtgta cctcttttca ataatatatt ttatattata tatgaaaaaa agaccaccga
     1801 cttaagtgag cggtggtcat tttgtttctc taattagtta acaggataaa cgctaacacg
     1861 tttgcgtgtt ttaccaaagc gttcaaaacg gacaatacct tcaacagtcg cgtagattgt
     1921 atcatcacta cctcttctgg cgttaagtcc tggataaatc cgagtacctc tttggcgata
     1981 taaaattgcc ccagctttcg ctagttgacc atcagcgcgt ttagcaccta agcgacgacc
     2041 agtggagaca cggccgttac gcgttg
//
LOCUS       DAOPCD010000168         2060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_42631_length_2060_cov_3.243392, whole genome shotgun sequence.
ACCESSION   DAOPCD010000168 DAOPCD010000000
VERSION     DAOPCD010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42631_length_2060_cov_3.243392"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PLQ55_04260"
     CDS             <1..946
                     /locus_tag="PLQ55_04260"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HPK29176.1"
                     /translation="DKLESIPGTRIDIPNDDFIRQVNEVGCAIIGQTGHLVPADKKLY
                     ALRDVTATVEAIPLIASSIMSKKLASGSDTILLDVKFGSGAFMKTLKDAQELARAMVD
                     IGKSLNRDTRAILTDMDQPLGLAVGNALEVKEAIATLKGEGPRDLSELCIEAACIMLE
                     QAGVVKKAQDARKEVENKIKSGAALQKLVDMVKAQGGDESYITNPEKFAISQNVFEVL
                     ATSEGYVEHIDSMAIGISSMKLGGGRETYEDEIDMSAGIVLNKKVGDKVKVGERLALV
                     YTNKADEVSTPILVDIHHAFKLSDKHIDVPPIIHDYIK"
     gene            946..1389
                     /gene="dtd"
                     /locus_tag="PLQ55_04265"
     CDS             946..1389
                     /gene="dtd"
                     /locus_tag="PLQ55_04265"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012574408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPK29177.1"
                     /translation="MRVVIQIVKDAKLEIDNKVYSSIDYGMLLLVSFTLSDTKEVAKK
                     MAEKIALMRIFPDENGKTNLNINQVNGEVLSVSQFTLYGEFNSRRPSFTKVLSGEESS
                     QLYDYFNSELRKMLKVKTGVFGSDMNITFTNVGPVTYVVESDEKR"
     gene            1376..>2060
                     /gene="mnmA"
                     /locus_tag="PLQ55_04270"
     CDS             1376..>2060
                     /gene="mnmA"
                     /locus_tag="PLQ55_04270"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPK29178.1"
                     /translation="MKKDKANIKVMVGLSGGVDSAVALALLKKEGYDVEAAFMRNWDS
                     LLNNDVSGNPTLYDPTCPQEVDYNDAKAVAETLGVKLHRVDFVKEYWDNVFSYFLSEY
                     EVGRTPNPDIFCNKYIKFDAFLAWALEMGADYIATGHYAKLEEVNGELVMKKSADKNK
                     DQTYFLSQLSQAQLKRTLFPLGDIPKSKVREIAEKLDLSPAKKKDSIGICFIGERHFR
                     DFLKNYIPAQ"
BASE COUNT          680 a          321 c          459 g          600 t
ORIGIN      
        1 tgataaactt gaaagtattc caggtacgcg tattgatatt ccaaatgatg actttattcg
       61 gcaagttaat gaagtaggtt gtgcgattat tgggcaaacg gggcacttag tacccgctga
      121 taagaagtta tatgcgcttc gtgatgtgac agcaactgtt gaagctattc cgcttattgc
      181 tagttccatt atgtcaaaga aacttgcttc cggcagtgac acgattttac ttgatgttaa
      241 atttggtagc ggtgcgttta tgaagacact aaaagacgcc caagaattag cgcgagcgat
      301 ggttgatatt ggtaagagtt taaaccgtga tacacgggct attttaacgg atatggatca
      361 accactaggc cttgcagtcg gtaatgcact tgaagtcaaa gaagcaatcg ccacgcttaa
      421 aggcgaagga ccgcgtgact taagtgaatt atgtatcgaa gcagcgtgta ttatgcttga
      481 acaagcaggt gtcgtaaaaa aagcacaaga cgctcgcaaa gaagttgaaa ataaaattaa
      541 gtcaggtgct gcgctccaaa aacttgtcga tatggtgaaa gcacaaggtg gtgacgagag
      601 ttatattact aacccggaaa aatttgcgat tagtcaaaat gtgtttgaag tattagcgac
      661 tagtgaaggt tatgttgaac atattgatag tatggcaatt ggtatttcta gtatgaaatt
      721 aggtggtggc cgcgaaactt atgaagatga gattgatatg tccgccggaa ttgttttaaa
      781 taaaaaagta ggagataaag tgaaagttgg tgaacgccta gcgctcgtat atacgaataa
      841 agctgatgaa gtttctactc caatcttagt tgatattcat catgccttta aacttagcga
      901 taagcatatt gatgtcccac caattattca tgattatatt aagtaatgcg tgttgttatt
      961 caaattgtta aagatgctaa attagaaata gataataaag tgtattcaag tattgattac
     1021 ggaatgcttt tacttgtatc ttttacgctt agtgacacta aagaagtagc gaaaaaaatg
     1081 gcggaaaaga tagcacttat gcgtatattt cccgatgaaa acggaaaaac taatttaaat
     1141 attaatcaag ttaatggcga agttttaagt gttagtcagt ttactcttta tggtgaattt
     1201 aatagcagaa gaccttcttt taccaaagtc ttaagcgggg aagaaagtag tcagctctac
     1261 gactacttta atagcgaact tagaaaaatg ttaaaagtta aaactggggt atttggcagt
     1321 gatatgaata tcacattcac taatgttggc ccagtgactt atgtggtgga aagcgatgaa
     1381 aaaagataaa gctaatatta aggtaatggt tggccttagt ggcggcgttg atagtgccgt
     1441 tgctttagcg ctcttaaaaa aagaaggcta cgatgttgaa gcggctttta tgcggaactg
     1501 ggactcactt ttaaataatg atgttagcgg caaccccacg ctttacgacc caacttgtcc
     1561 gcaagaagtt gattataatg acgcaaaagc agtagcagaa acgcttggcg ttaaacttca
     1621 tcgtgtcgac tttgttaaag aatattggga caatgttttt agttattttt taagtgaata
     1681 tgaagttggt cgcaccccaa atccagacat cttttgcaat aaatacatca aatttgacgc
     1741 gtttttagcg tgggcattag agatgggcgc agattatatt gcaaccgggc actacgctaa
     1801 gctagaagaa gtaaacggcg aacttgttat gaaaaaaagc gcagataaaa ataaagatca
     1861 aacttatttc ttaagtcagc tctcacaagc gcaacttaag cgtactttat tcccacttgg
     1921 ggatatccct aaaagtaaag tacgggaaat tgctgaaaag cttgatttaa gtcctgctaa
     1981 aaagaaagat agtatcggga tctgttttat cggtgaaaga catttccgtg attttttaaa
     2041 aaattacata ccagcgcaag
//
LOCUS       DAOPCD010000169         2032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_43432_length_2032_cov_3.166414, whole genome shotgun sequence.
ACCESSION   DAOPCD010000169 DAOPCD010000000
VERSION     DAOPCD010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43432_length_2032_cov_3.166414"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1402
                     /gene="xseA"
                     /locus_tag="PLQ55_04275"
     CDS             146..1402
                     /gene="xseA"
                     /locus_tag="PLQ55_04275"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPK29179.1"
                     /translation="MRSILSISELNAYVKNVIQRDAFLSYVEVRGEIINLKRYNTAIY
                     FTLKEEDKARLSVVSFNTKAFPTTLKDGDEVIATGRITVYEKSGNYQLVATEVSYYGL
                     GAKLLELAKLKVKLDKMGLFDQEKKRPLPKFPKVIGIITPKDSAAQSDLVTNINRRYP
                     LVTLKIVNATFQGESAPSSLISAFEVLQNEHIDVLIIARGGGSSDDLWAFNDESLIMA
                     LSAKKVPLISAIGHEIDTTLVDYLADVRASTPTAAAELVVPDQNELRQTLTDYSVRLE
                     NAMSTVLIQREKELNHLIIRPVLVNFGAIVENLTTKLLTLSTKLGARSAENIARAEII
                     LHSLKERLNKAITRTYNVINETLVAKSTRLEALNPTNVLNRGYAFISKNEEIIKSAKK
                     LKVKDEVKITFSDGSVLAEVKGGEDE"
     gene            1395..1586
                     /gene="xseB"
                     /locus_tag="PLQ55_04280"
     CDS             1395..1586
                     /gene="xseB"
                     /locus_tag="PLQ55_04280"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPK29180.1"
                     /translation="MSKEKLSFEQKLARLNAIVSELESGKLSLDASLKLFEEGNALSK
                     ELATELNEAKLKIEELQGK"
     gene            1745..>2032
                     /locus_tag="PLQ55_04285"
     CDS             1745..>2032
                     /locus_tag="PLQ55_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPK29181.1"
                     /translation="MSKVILHIDLNAFFAACEVLRDPTLKGKPLIVGGANRRGIVSTA
                     SYEARKYGIHSAMPTYKALRLCPNLIIKEPDFKLYHHYSSLFFNYLKDHVSP"
BASE COUNT          681 a          330 c          401 g          620 t
ORIGIN      
        1 atagttacgc tgtttatttt tatactgaag ataaaggtaa aagtaaagca gtgattatta
       61 atgtcgctat taaattatca aaacttttta atccgaccga agattataaa tttattaatg
      121 ctgtgcttga tggggtttta atttaatgcg aagtatttta tcaattagtg aacttaatgc
      181 atacgttaaa aatgttattc agcgtgacgc atttttaagt tatgttgaag tacgcggaga
      241 aattattaac ttaaaacgct ataatactgc gatttacttc accttaaaag aagaagataa
      301 ggcacgcctt agtgttgtaa gttttaacac taaagcgttt cctacgacgc taaaagatgg
      361 tgacgaagta attgccacag gtcgtattac ggtttatgaa aaaagtggta actaccaact
      421 cgtggccact gaagttagtt attacggact tggtgcgaag ttgcttgaac ttgctaaact
      481 taaagtaaag ttagacaaga tggggctttt tgatcaagaa aagaagcgcc cattacctaa
      541 atttcccaaa gttattggta ttattacccc aaaagatagc gcggcacaga gtgatttagt
      601 gacgaatatt aaccgtcgct atccgcttgt gactttaaag attgttaacg ctacttttca
      661 aggggaaagt gcgccttctt cactcattag tgcctttgaa gttttacaaa atgaacatat
      721 tgatgtctta atcattgccc gtggcggtgg aagtagtgac gacttatggg catttaatga
      781 tgaaagtctc attatggcct taagtgcaaa gaaagtccca cttattagtg cgataggaca
      841 tgaaatcgat acaacattag tcgattatct tgccgatgtg cgcgctagta ccccaactgc
      901 tgccgccgaa cttgttgttc ctgatcaaaa tgaattacgg caaacgctta ctgattatag
      961 tgttcgtctt gaaaacgcta tgagtactgt gctcatacaa cgcgaaaaag aactgaatca
     1021 cttaataata agaccagtac ttgtaaactt tggcgcaatt gtggaaaact taacgacaaa
     1081 gcttttgaca ctgagcacta aattaggagc ccgtagcgcc gaaaacatcg ctcgagcgga
     1141 aattatcttg cattcattaa aagaacgctt aaataaggcg ataactcgta cgtataatgt
     1201 tattaatgaa acgcttgtcg ctaagagtac acgacttgaa gcacttaatc cgacgaatgt
     1261 attaaataga ggctatgctt ttattagtaa gaatgaagaa attattaaat cagcgaagaa
     1321 gttaaaagta aaagacgaag ttaagattac atttagtgac ggcagtgtac tagccgaagt
     1381 taaaggagga gaagatgagt aaagaaaaat tatcatttga acaaaaactc gcacgcttaa
     1441 atgctattgt tagtgaacta gaaagcggga aacttagttt agacgctagt ttaaaactct
     1501 ttgaagaagg caacgcttta agtaaagaac tagcgacaga attaaatgaa gcaaaactga
     1561 aaattgaaga attacaagga aaataacgct aaaacgaccc tttagtatgt tattagtaat
     1621 tagttagtag tcttttagtt gctttatagt gttataaaat aggacattcg ttcctatttt
     1681 ttattaggaa cttattattt acataattat tatgtaaacc aaaatagtga gttataatat
     1741 atttatgagt aaagttatct tacacattga ccttaacgct ttctttgccg cgtgcgaagt
     1801 actgcgtgac cctaccctaa agggtaaacc tttaattgtt ggtggggcta acaggcgcgg
     1861 aatcgttagc acggcgagtt acgaagctcg gaaatacggt attcattcgg ccatgccgac
     1921 atataaagcg ctgcgcttgt gtccaaattt aattattaaa gaacctgatt ttaagcttta
     1981 tcatcattat tcttcgctct tttttaatta tttaaaagat catgttagtc cc
//
LOCUS       DAOPCD010000170         2026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_43609_length_2026_cov_2.454084, whole genome shotgun sequence.
ACCESSION   DAOPCD010000170 DAOPCD010000000
VERSION     DAOPCD010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43609_length_2026_cov_2.454084"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..568)
                     /locus_tag="PLQ55_04290"
     CDS             complement(62..568)
                     /locus_tag="PLQ55_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29182.1"
                     /translation="MKIKIADGIKLHQTLTAEVDLSDVDLHANYSLKAIKPFTVTVDF
                     DTFTDVVEVHIRGKVTLILECAYTLEHFDYEVEMDDELTFNFVNPNIEAETDDCFYEK
                     GPEIFLDPYLYAIILSYIPLKVIKPGANLPKSGEGYEILSEEEFEAKHQTLDDEFGDL
                     FDKLDLDD"
     gene            complement(565..>2026)
                     /locus_tag="PLQ55_04295"
     CDS             complement(565..>2026)
                     /locus_tag="PLQ55_04295"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPK29183.1"
                     /translation="DAEKVLCLGGINGAKSSAVTNETKRIAIEVAAFDTAHIRMSVVR
                     HNLPSEASTRFGKGVNLNNTTEAMNLALSLIFDLVPGAEFSNTVTVEKSLPAQPVIYF
                     DKDKINKLLGTTFSEEVMLSTLNRLNITVNEDKSVNLPLYRQDFFSLADLAEEIIRLL
                     GFEHVGNEIPALFTTVGSYDEVQRKRYDIRKLLRSKGLYEVLTYALTDEEKNKTFVYL
                     NKDVSLKLLHPMTPVRSELRLNLLPSVIETLTYNASRQQSDFALFEISNITSENYKGE
                     HLAFAFYGNEVLSRELSKRPYDFFDAKGLIISVLEYLGINESRYEFVFNPHKNEEFHP
                     AQSALLKVQGKVVGVFGKLHPLAKEKALLNNEAIIGELNLSALLSLKTSLNKVNPPAR
                     FPFVRRDLALLVNEDLSVKELINVIRKAGGKLISDVSVFDVYTKLKDEPGKKSVALTL
                     TLQDANKTLVEDEIKEVMTKVIEELTKKLQVVVRGI"
BASE COUNT          629 a          399 c          308 g          690 t
ORIGIN      
        1 tgcaaaaaaa caaaaaagaa gagttgcccc ttcttttatg ctcattctat gagaataata
       61 attaatcgtc taaatctagc ttatcaaaga gatcaccaaa ttcatcatca agcgtttggt
      121 gtttagcttc aaattcttct tcacttaaga tttcataacc ttcgccactc tttggtaaat
      181 tagcgccagg cttaataact ttaagcggga tataacttaa gataattgca tataagtatg
      241 gatctaaaaa gatttccggt cctttttcat aaaaacagtc atccgtttct gcttcaatat
      301 tcggattaac aaagttaaaa gtaagttcgt catccatttc tacttcataa tcaaaatgtt
      361 ccaaagtata ggcacattct aaaataagtg tgactttgcc tctaatatga acttccacaa
      421 catcggtaaa tgtatcaaag tcaacagtta cggtaaaagg tttaatcgct ttaagcgaat
      481 aattagcgtg taaatcaaca tcacttaaat caacttctgc tgttagagtt tgatggagtt
      541 taattccatc agctattttt attttcatat tccgcgtacc actacttgta attttttcgt
      601 taattcttca ataactttag tcatcacttc tttaatctca tcttcaacta atgtcttatt
      661 agcgtcttgt aaggtaagtg tcagcgctac tgatttttta cccggttcat cttttagttt
      721 cgtgtaaaca tcaaatacac tgacgtcact tattaactta ccaccagctt tacgaataac
      781 attaattaat tctttaacac ttaagtcttc attaacaagg agcgctaagt cacgccttac
      841 aaacgggaac cttgccggtg ggttaacttt atttaagctt gttttaagac taagcaatgc
      901 acttaaatta agttcaccaa taattgcttc attattaagt aatgcttttt cttttgcaag
      961 ggggtgaagt ttaccaaaaa caccaactac tttaccttga acttttaaga gtgcgctttg
     1021 cgcaggatgg aactcttcat tcttatgcgg attaaacaca aattcataac gcgattcatt
     1081 aatccctaaa tactctaaca cactaataat gagcccttta gcgtcaaaaa agtcatatgg
     1141 tcttttactt aattcacgac ttaacacttc attaccatag aatgcaaacg ctaggtgttc
     1201 acctttataa ttttctgaag ttatgttact aatttcaaaa agcgcaaaat cactttgttg
     1261 ccgactagcg ttataagtaa gtgtttcaat aacgcttggt aaaagattaa gtcgtaactc
     1321 acttctgact ggtgtcatcg ggtgcagtaa ttttaaagat acatctttat ttaagtacac
     1381 gaatgtttta tttttttctt catcagttaa ggcataagtt aacacttcat aaagcccttt
     1441 actgcgtaat aatttacgaa tgtcataacg ctttctttgt acttcgtcat aactcccaac
     1501 agtcgtaaat aatgcgggaa tttcgttacc gacatgttca aagcctaaaa ggcgaataat
     1561 ttcttcggct aagtcggcta gagaaaagaa atcttgccgg taaagtggga gattaacact
     1621 cttatcttcg ttcaccgtaa tatttaaacg gttaagtgta cttagcatta cttcttcact
     1681 aaaagtagtg cccagtaact tattaatctt atctttatca aagtaaatta caggttgcgc
     1741 tggtaaactc ttttcgactg taactgtatt actaaattca gcaccaggca ctaagtcaaa
     1801 gataagcgag agcgctaaat tcattgcttc agttgtgtta tttaaattaa cacctttacc
     1861 aaagcgcgta cttgcttcac ttggtaaatt gtgacgcact acactcatcc gaatatgcgc
     1921 cgtgtcaaaa gctgctactt caatggcaat gcgttttgtc tcatttgtga ctgctgaact
     1981 tttagcgccg ttaattcccc ctagacataa tactttttca gcgtcg
//
LOCUS       DAOPCD010000171         2004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_44271_length_2004_cov_3.947152, whole genome shotgun sequence.
ACCESSION   DAOPCD010000171 DAOPCD010000000
VERSION     DAOPCD010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44271_length_2004_cov_3.947152"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..402
                     /locus_tag="PLQ55_04300"
     CDS             160..402
                     /locus_tag="PLQ55_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPK29184.1"
                     /translation="MSDTLTLDKLTIGQKARVLRINDDNPALKQRLLDMGITTGVIVK
                     IDKISPLGDPFCLELRNYRLLVRRADVSAIEVEVIE"
     gene            399..>2004
                     /gene="feoB"
                     /locus_tag="PLQ55_04305"
     CDS             399..>2004
                     /gene="feoB"
                     /locus_tag="PLQ55_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPK29185.1"
                     /translation="MKIALIGNQNSGKTSLFNLLTGNNQKVGNWPGVTLERKTGILRT
                     SNAEVIDLPGIYSLSPYSHEENIVRRFLLEEKVDLIINIIDSTAIERSLYLTTQLFNF
                     NIPMVIALNMTDLAFEQGIRIDVNKLSKALSVPVVPISCLKRTGIKDLIMLITKDTPT
                     STNYLTIYSNPLQETINKTAELLPIDAKEFLAIKLLENDIDLAPRIDLDVSKDIKKLN
                     DHYKLGISEVLANERYNFIEKVRDIAVSSREKPKLLTDKIDRILLNKYLAIPIFIIIM
                     LLVYFLAAGPVGIFLSSLVEQGFAALNEGLATFLTNVGASAWSVSLVVDGILTGVTSI
                     LSFVPQLLILFFMIDILEASGYMSRIAFFFDRVFRKVGLSGKALIPFIIGTGCSVPAI
                     MASRVIEDERERKLTVLLTPFIPCGAKLPIITLFAAYFFPDSMGLVSASLYFLSIIII
                     LLTALLLKRILYKQKYSPFIQELPTYKAPNMRYVLRDTGHKTWSFITNAGSVILFASV
                     ILWLLLNFNWKMQFGVAIEKSILSDIG"
BASE COUNT          626 a          339 c          361 g          678 t
ORIGIN      
        1 taaacattat aatgaagaca agaaggatgt aaatttaacc ttttccttta acggttaaca
       61 tatatttatt atgttaaata cttgataata agcggctatt agggtatatt ttggtgtgtg
      121 attatacttt gtataaccac tagaagggat tattcctaag tgagcgatac tcttactcta
      181 gataaactta ccatcgggca aaaagcccgt gttttaagaa ttaatgatga taatccagca
      241 ttaaaacaac gacttttaga tatgggaatt acgactggtg tcattgttaa aattgataaa
      301 atatcaccac ttggtgaccc tttttgccta gagttacgca actatcgctt attagtgcgt
      361 cgcgctgatg tgagtgcgat tgaagtcgag gttatcgaat gaaaattgca ctaattggta
      421 atcaaaatag tggtaaaact tcactattta atttgttaac tggtaataat cagaaggttg
      481 gtaattggcc aggagtaacg cttgagcgca aaactggtat tttacgcacc tcaaatgccg
      541 aagtgattga tttaccaggt atctatagtc tctctccata tagtcatgaa gaaaatattg
      601 tccggcgctt cttattagaa gaaaaagttg acttaataat taacattatt gatagtaccg
      661 cgattgaacg tagcttatat cttactacgc aattatttaa ttttaatatt ccaatggtca
      721 ttgcactaaa catgactgac ttagcgtttg aacaagggat tcggattgat gtaaataaac
      781 ttagcaaggc actaagtgtt cccgtagtcc caatatcatg tttaaaaaga acagggataa
      841 aagatttaat aatgttaatt actaaagata cccctacttc tactaattac ctaactatat
      901 atagcaatcc actgcaagaa actatcaata aaacagccga acttttaccg attgacgcta
      961 aagaattttt agcaataaaa ctattagaaa atgatattga tttagcgcca cgcatcgatc
     1021 ttgatgtaag caaagacatt aaaaaattaa atgatcatta caaacttggg ataagcgaag
     1081 tattagcgaa tgagcgctat aattttattg aaaaagttcg tgatattgct gtttcaagcc
     1141 gcgaaaaacc aaaactttta acggataaaa ttgatcggat tttactaaat aaatatctag
     1201 caattccgat atttattatt atcatgttac ttgtctattt cttagcagca ggaccagttg
     1261 gcatttttct ctcatcacta gttgagcagg gttttgccgc gttaaacgaa ggtttagcaa
     1321 cttttctcac taatgttggg gcttctgcgt ggtctgttag tttagtcgtt gatggcatct
     1381 taacaggcgt gacttcgatt cttagttttg tgccgcaatt actcatttta ttctttatga
     1441 ttgatatctt agaagcaagt ggttatatgt cccgcattgc tttctttttt gatcgtgttt
     1501 tccgtaaagt tggtttatcg ggtaaagcac ttatcccttt tattattggt acgggatgca
     1561 gtgtaccggc gattatggca agccgcgtta ttgaagatga acgcgaacga aaattaactg
     1621 ttttacttac gccttttatt ccgtgcggcg caaaattacc gattattacg ctctttgcgg
     1681 cctacttctt ccctgattca atgggactag taagtgcctc attatatttc ctatcaatta
     1741 tcattatttt actaacagct ttgcttttaa aacgcatcct ttataagcaa aagtattcac
     1801 catttattca agaactacca acttataaag caccaaatat gcgctatgta ttacgtgata
     1861 caggacataa aacatggtca tttatcacga atgccggctc agttatttta ttcgcctctg
     1921 ttatcttatg gcttttactt aactttaact ggaagatgca atttggcgtt gcgattgaga
     1981 agagtatttt aagtgatatt ggac
//
LOCUS       DAOPCD010000172         1972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_45259_length_1972_cov_4.663537, whole genome shotgun sequence.
ACCESSION   DAOPCD010000172 DAOPCD010000000
VERSION     DAOPCD010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45259_length_1972_cov_4.663537"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..582)
                     /locus_tag="PLQ55_04310"
     CDS             complement(34..582)
                     /locus_tag="PLQ55_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPK29186.1"
                     /translation="MKLATKRLILRPFTLNDVDAVQKYSSKESVTRYMMWGPNKYEDT
                     KRFVSSVVSRNSETPRDVFDFIVTLKDGTVIGACGLYFDEYPKPPTLGWVFDDTYWNQ
                     GYGTETGRALLAFGFNTLKVKSILATCDNENIASARIMEKIGMRYVETRPEELPKLGK
                     RFIKVYEITALEYKLNSNKVLL"
     gene            723..1385
                     /locus_tag="PLQ55_04315"
     CDS             723..1385
                     /locus_tag="PLQ55_04315"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HPK29187.1"
                     /translation="MKQKKINNLIIDYIFNNYDKETPIFTKDIYQKYSEISRSTIRSI
                     LSRLVKENNIVRIAEGIYSLPNYDCAIGTIAISNNEIINKKYIRNDGKIFGYRTGISF
                     ANKLGLTTQTAGVDSIISNCVANKKREVKLNKTRVIVNSPRVEVTANNYKLLQILDLL
                     NDYTSFSEINLEEGASTIFKYLEGVNLERKEVEKIVNSYPREAQVNFYKIRCQDAITS
                     KY"
     gene            1363..>1972
                     /locus_tag="PLQ55_04320"
     CDS             1363..>1972
                     /locus_tag="PLQ55_04320"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPK29188.1"
                     /translation="MLLHQNIKEFRKIVILTATHYGLQEYQIEKDYYISLFLKPLGNM
                     SKNIPIVFKGGTSLSKCYGVISRFSEDIDLSVIQNKQLTRKQKKDLKQLIIETAKEIG
                     AEVININEIESRKHFNRYILKFNSVFNSDVDTLQKLIIETMIAYNPFPAIYQKTENLI
                     LEYLKIQKKNDIIGQYQLDSFLFLTQSIERTFVDKIFALCDYH"
BASE COUNT          746 a          289 c          292 g          645 t
ORIGIN      
        1 ttaaaagtat taaaacacta ttaatcctta atttcataaa agcaccttgt tactatttaa
       61 tttatattca agcgcggtta tttcataaac tttaataaat cttttcccta gtttaggtaa
      121 ttcttctggt ctagtttcaa catagcgcat accgattttt tccataatgc gggcactagc
      181 gatattttca ttatcacagg tggcaagaat gcttttaact ttaagtgtat tgaagccaaa
      241 cgctaataga gctctgcctg tttccgtgcc ataaccttga ttccaatagg tatcatcaaa
      301 tacccaccct agcgttggtg gttttgggta ttcatcaaaa taaagaccac aagcgccaat
      361 aacggtgccg tcttttaatg taacaataaa atcaaaaaca tcacgcgggg tttcactatt
      421 tcgactaaca actgaagata caaatcgttt ggtgtcttca tatttatttg gtccccacat
      481 catatagcga gtaacacttt ctttacttga atatttttgc acagcatcaa catcgtttag
      541 tgtaaaaggt cttaagatta gtcgtttagt cgctaatttc atgatgttac ctccgtataa
      601 tgttaattat atcaatactt tattatttaa aattgatttt aaatgcaaca gcaagtaaac
      661 attaataaga aaaagtgttg caaagcttag tagagatgat aaaatgtaga cgcagaatag
      721 cgatgaaaca aaaaaagatt aataatttaa ttatagatta catatttaat aactatgata
      781 aagagacacc aatttttact aaagatatat atcaaaaata tagtgaaatt agtagaagta
      841 caatccgctc tattttaagt cgtttagtaa aggaaaataa tatcgttaga attgcagaag
      901 gtatttactc tttaccaaat tatgattgtg ctatcggtac catagctatt tccaataatg
      961 aaattattaa taaaaaatat attcgaaacg atggaaagat attcggttat cgtacgggca
     1021 taagtttcgc taacaaatta gggttaacaa cgcaaactgc aggagttgat tcaataatct
     1081 caaattgtgt cgctaataaa aagcgtgaag tgaaattaaa taaaacacgt gttattgtta
     1141 atagtccgcg tgtagaagtc acagcaaaca attataaatt attacagatc cttgaccttt
     1201 taaatgatta cactagtttt agcgagataa atttagaaga aggggcaagc accattttta
     1261 aatatttaga aggtgttaat ttagaaagaa aagaagtaga aaaaatagtt aatagctacc
     1321 cgcgtgaagc acaagttaat ttttataaaa ttaggtgtca ggatgctatt acatcaaaat
     1381 attaaagagt ttagaaaaat tgttattcta accgctactc attatgggct ccaagaatat
     1441 caaatagaaa aagattatta catatcatta tttttaaaac cgcttggtaa tatgagtaaa
     1501 aacatcccta tagtttttaa aggtggtacc tctttatcta aatgttatgg agttattagt
     1561 cgtttttctg aagatattga cctttctgta attcaaaaca aacaactaac cagaaaacaa
     1621 aaaaaggatc ttaaacaatt aataatagaa acagcaaaag aaattggtgc agaagtaata
     1681 aacataaatg aaattgaatc aagaaaacat tttaatcgct acattctaaa atttaacagt
     1741 gtctttaaca gtgatgtcga tacacttcaa aaacttatta ttgaaacaat gattgcctat
     1801 aatccatttc ctgcgattta ccaaaaaaca gaaaacttaa ttttagaata ccttaaaatt
     1861 cagaaaaaaa acgatataat tggacaatat caacttgatt cttttttatt tttaacgcaa
     1921 tctattgaaa gaacttttgt tgataaaata tttgcgttat gtgattatca tt
//
LOCUS       DAOPCD010000173         1970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_45322_length_1970_cov_3.910183, whole genome shotgun sequence.
ACCESSION   DAOPCD010000173 DAOPCD010000000
VERSION     DAOPCD010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45322_length_1970_cov_3.910183"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /gene="uvrA"
                     /locus_tag="PLQ55_04325"
     CDS             <1..1357
                     /gene="uvrA"
                     /locus_tag="PLQ55_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019715858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPK29189.1"
                     /translation="QRIRLATQIGSKLTGVLYVLDEPSIGLHQRDNNKLIQALRDMVD
                     LGNTLIVVEHDEETIRSADHIIDIGPGAGIHGGEVVVTGTVEDVINCKKSITGDYLSG
                     RKVIPLPPRRMPGNGNYLKIYGAAANNLRHIDVSIPLNTLTLVTGVSGSGKSSLVEEV
                     LFKAVDGHLRRVETYPGAYSKIEGLEHIDKVINIDQDPIGRTPRSNPATYTKVFDDIR
                     ELFAQTVDARARGYTKSQFSFNTKGGRCEHCEGAGVTRIAMNFLPDVYVKCEMCDGQR
                     YHADTLSIHYRGKNIVDVLNMTVEEALEFFANRPQIVRRLQTLYDIGLGYIKLGQPAP
                     ELSGGEAQRLKLAFELQKRPTGKTLYLLDEPSTGLHADDVGRLITSLQAIVANGDTVI
                     VIEHNLDIIKVADYIIDLGPGGGNYGGKVVASGTPEEIVKVKGSYTGEYLAPYLKKEK
                     K"
     gene            1359..>1970
                     /locus_tag="PLQ55_04330"
     CDS             1359..>1970
                     /locus_tag="PLQ55_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29190.1"
                     /translation="MDLTLVVVVIFGLIFIGAVTVLGLSLNEFVKKEEDINTLFKGKH
                     RLIAISVLSGAVSVLMLFLPLMVLSNSVLHSLLIGLGSFLFALMLLTFIAAFVLHYYK
                     FNVLQREWIKESKIVTIISGVLSIVFLFVLLEGLTLAEIIKFPLPRGIPFGDSPVIAF
                     YAIFILTGALLVLAITDHEFYKKYGRHGILENVFYVAFPAGIIG"
BASE COUNT          583 a          312 c          446 g          629 t
ORIGIN      
        1 gcagcgcata agacttgcga cgcagattgg tagtaaatta accggtgttt tatatgtgct
       61 tgatgaacca agtatcggtc ttcatcagcg tgataataat aaattaatcc aagcattacg
      121 cgacatggtt gatttaggga atacactaat tgttgtcgag cacgatgaag aaacaattag
      181 aagcgctgat catattattg atattggtcc aggagcgggg attcatggtg gtgaagtagt
      241 ggtaacgggc actgtggaag atgtgattaa ttgtaaaaaa tcaattactg gtgattatct
      301 ctcgggccgt aaagtaattc cgttaccgcc tagacggatg cctgggaacg gtaattactt
      361 aaaaatatat ggcgcagcgg cgaataactt aaggcatatt gatgtaagta tcccgcttaa
      421 taccttaaca cttgtcacgg gtgtgagcgg ttcaggcaaa tcaagtttag tggaagaagt
      481 actttttaag gcagtggacg gtcatttacg tagagttgaa acttatccag gtgcttactc
      541 taaaattgaa ggcttagaac atattgataa agtaattaat attgatcaag acccaattgg
      601 tagaacaccg cgcagtaacc ctgcgactta tacgaaagtg tttgatgaca ttcgtgaact
      661 ttttgcgcaa actgtggacg ctagagcgcg aggttacacg aagagtcaat tttcgtttaa
      721 cactaaaggt ggtagatgtg aacactgtga aggggcgggt gttacacgca ttgctatgaa
      781 ctttttacct gatgtctatg taaaatgtga aatgtgtgac ggacaaagat atcacgctga
      841 cacgctttcg attcattatc gcggcaaaaa cattgttgat gtacttaata tgacagtcga
      901 agaagcgctt gaattctttg caaataggcc gcagattgta cgccgtttac aaactttata
      961 cgatattggt ttaggttata ttaagcttgg acaacctgca ccagaactta gtggcgggga
     1021 agcacaacgc ttaaaacttg cctttgaatt acaaaagcgt ccgactggta aaacactata
     1081 tttacttgat gaaccgtcaa ccggcttaca tgcagatgat gttgggcggc ttattacttc
     1141 actccaagct attgtcgcta atggggatac tgttattgtt attgagcata atcttgatat
     1201 tattaaggtt gctgattata taattgattt aggtccaggc ggtgggaatt atggtggaaa
     1261 agttgtcgct agtgggaccc cagaagaaat agttaaagtt aaagggtcgt atactggaga
     1321 atatttagcg ccctacttaa aaaaggagaa aaagtaatat ggatttaaca ctcgtggtag
     1381 ttgtaatatt tggcttgatc tttattggag cagtgacggt ccttggttta agtcttaatg
     1441 aatttgtaaa aaaagaagag gatattaata ccttgttcaa aggtaaacac cgtttaatcg
     1501 ccattagtgt cttaagcggt gcagtgtcag tgttaatgct gtttttacca ctgatggtgc
     1561 tatcaaatag tgtgttacat tctttgttaa ttggtttagg tagttttctc tttgctttaa
     1621 tgttactcac ttttattgct gcttttgttt tacactatta taaatttaat gtgttacaaa
     1681 gagagtggat aaaagaaagt aaaatagtaa cgattataag cggtgtttta agtattgttt
     1741 tcctttttgt attgcttgaa ggcttaacac ttgcggaaat tataaagttc ccattaccac
     1801 gcggtatccc ttttggtgat agtcccgtta tcgcttttta cgcaatattc attctaactg
     1861 gtgcgctttt agtattagcg attactgacc acgaatttta taaaaaatac ggtagacacg
     1921 gtattttaga aaatgttttc tatgttgctt ttccagcggg aattatcggc
//
LOCUS       DAOPCD010000174         1966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_45463_length_1966_cov_4.552590, whole genome shotgun sequence.
ACCESSION   DAOPCD010000174 DAOPCD010000000
VERSION     DAOPCD010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45463_length_1966_cov_4.552590"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="PLQ55_04335"
     CDS             <1..1155
                     /locus_tag="PLQ55_04335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPK29191.1"
                     /translation="DLDLLRKALKVMDQGVEKHRQGQVGDVNDYLSDLLRDITKVAEE
                     RRIMDFESLGDIAKGLKAHLEKMRKSSGNKLTGNSTGFYDIDRYTHGFQEDNYIIIAA
                     RPAVGKTAFGLSLAYNMAKINNKTVGFFSLEMGNQQLMQRLVSKVSGVLHKNIMTGYL
                     SKEERVKVYETIENLKHIKLYIDETPSININDLLIKARKLKRENDDLCAIFVDYIGLI
                     TTTGHVESRQIELSEISAALKGLARELNITIVALSQLSRAVEATKDKMPQMSHLRESG
                     SLEQDADLVFLMYREDYYVKQGTIKEIDAVYQPYYQTLAQAKAEMKDPSKDLDVSPVD
                     IILAKNRSGETGIATLLFFKAISSFDNPGPDLTARFTEYRTKKGPSYKHE"
     gene            1152..1910
                     /locus_tag="PLQ55_04340"
     CDS             1152..1910
                     /locus_tag="PLQ55_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPK29192.1"
                     /translation="MKYFVLASGSKGNATIIKGPETKILIDLGLSLKEYKERLNVFKL
                     QEERVFAVLFTHKHGDHITSQFNKVANGNVYAPANTLPFEAPFKLVVPYTPFTINEFK
                     VTPLPTSHDVSDSVGYIIETTEYKLVYMTDTGYISTRNLEMMKNADAYIIESNHSPSL
                     LLKTNRPFDLIQRILSDTGHLSNEACAHYLAELVGPRTKHIVLAHLSEEANTPEVALE
                     TLDKVFRKYGIDQSKLTIVTAKQHVPIMGELNEN"
BASE COUNT          681 a          354 c          381 g          550 t
ORIGIN      
        1 gatttagatt tacttcgtaa agccttaaaa gtaatggatc aaggcgttga aaaacaccgt
       61 caaggtcaag ttggtgatgt taatgattac ttaagtgatt tattacgcga cattactaaa
      121 gttgcggaag agcgacggat tatggacttt gaatcacttg gtgatatcgc taaagggtta
      181 aaagctcacc ttgaaaaaat gcggaaatct tctggtaata aactcacagg aaattcgacg
      241 ggcttttatg atatagatcg ttacactcac ggtttccaag aagataacta tattattatt
      301 gctgcacgtc ctgcggtcgg taaaacggca tttggtttaa gcttagccta taatatggct
      361 aaaataaata ataaaactgt tggtttcttc tcgcttgaaa tgggtaacca acaactaatg
      421 caacggcttg tttctaaagt ttcaggtgtg cttcataaaa atattatgac ggggtacctt
      481 agtaaagaag aacgcgtaaa agtttatgaa acaatcgaaa acttaaagca tatcaaactc
      541 tatatcgatg aaacaccaag tataaatatt aatgacttac ttatcaaagc acggaaatta
      601 aaacgcgaaa atgatgattt atgcgccatt tttgttgact atattggttt aattacgaca
      661 acgggacatg ttgaaagtag gcaaattgaa cttagcgaaa taagtgcggc cctgaaaggc
      721 ttagcgcgcg aattaaatat tacaatcgtt gccttatcac aactttcacg ggcagttgaa
      781 gccacaaaag ataaaatgcc acaaatgagt caccttcgtg aatcaggtag tctagaacaa
      841 gacgcagatt tagtgttttt aatgtatcgc gaagattatt atgtcaaaca aggaacgatt
      901 aaagaaatcg atgctgttta tcaaccctat taccaaacac tcgcacaagc taaagcggaa
      961 atgaaagacc cgagcaaaga tcttgatgtt tcaccagttg acattatctt agcgaaaaat
     1021 agaagtggtg aaacgggaat tgcgaccctg ctatttttca aggctatttc aagttttgat
     1081 aatccaggac cagacttaac agcgcggttt actgaatatc gcactaaaaa aggaccaagt
     1141 tataaacacg aatgaagtac ttcgtcctcg ctagcgggtc caaaggtaat gccacaatta
     1201 tcaaaggacc tgaaacaaaa atattaattg atttagggct cagccttaaa gaatataaag
     1261 aacgactaaa tgtctttaaa ttacaagaag agcgtgtttt tgccgtgctt tttactcata
     1321 aacacggtga tcacattact tcacaattta ataaagtcgc taacgggaat gtgtatgctc
     1381 cggcgaacac tttgccgttt gaagcaccat ttaaactagt tgtgccctac acccctttta
     1441 cgattaatga atttaaagta acgcccctac caacaagtca tgatgtaagt gattcagttg
     1501 gttatattat tgaaacaaca gagtacaaac ttgtgtatat gactgacact ggttatataa
     1561 gcactcgtaa cttggaaatg atgaaaaacg ctgacgccta cattattgaa tcaaatcata
     1621 gtccgagttt attacttaaa acaaatagac cctttgattt aattcagcga atactaagcg
     1681 atactggtca cttatcaaac gaagcttgtg ctcactattt agcagaactt gttggcccgc
     1741 gcactaaaca catcgtttta gcgcacctta gtgaagaagc taatactccc gaagttgcgc
     1801 ttgaaacact tgataaggta tttcggaagt acgggattga ccaaagtaaa ctgacaattg
     1861 ttaccgcaaa acaacatgtg ccaatcatgg gagagttaaa tgaaaattga tgttattgtt
     1921 gtcgggaaaa caaaaggtgc acttctaatt actagcgacg aataca
//
LOCUS       DAOPCD010000175         1957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_45753_length_1957_cov_3.756046, whole genome shotgun sequence.
ACCESSION   DAOPCD010000175 DAOPCD010000000
VERSION     DAOPCD010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45753_length_1957_cov_3.756046"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   56..1916
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=56..84
                     /rpt_unit_seq="gtgttagtaaacaatgatgggatttaaag"
BASE COUNT          638 a          241 c          480 g          598 t
ORIGIN      
        1 caaccgatgg atacaccacc tatcttccta aacacaactt ttgtttatat ctcaggtgtt
       61 agtaaacaat gatgggattt aaaggcagtt tttccgcgta gctgacaagc ttggcaaggc
      121 gtgttagtaa acaatgatgg gatttaaagg atgcgggcgg cgtgtaggcg aagctccttg
      181 cctcgtgtta gtaaacaatg atgggattta aagacgccgc cggcctgatt gtgcaggtgg
      241 cgccgggtag tgttagtaaa caatgatggg atttaaagca taagccgtct gcgcccgcgt
      301 tttgtccatt tccgcgtgtt agtaaacaat gatgggattt aaagaaaggg taacatatgt
      361 taagcgacaa agaaaatata gtgttagtaa acaatgatgg gatttaaagt gcagatgttt
      421 cagcggtaaa ctatctcggg ttttcggtgt tagtaaacaa tgatgggatt taaaggcggc
      481 ttacgttatg atgaagatta tggcggttta aggtgttagt aaacaatgat gggatttaaa
      541 gaaggtacgt ctactggcac ggcacgaatt tatggtttgt gttagtaaac aatgatggga
      601 tttaaagata gtttaatatg gtttgtaaaa caatatagca aagtgttagt aaacaatgat
      661 gggatttaaa gcaatattat aactctccat agccttgcgc caataaggtg ttagtaaaca
      721 atgatgggat ttaaagacaa taaatacaga taattgcgac tacgttgata taagtgttag
      781 taaacaatga tgggatttaa aggaatacaa agtatctcta gtattaattc cagtcggtcg
      841 tgttagtaaa caatgatggg atttaaagat tagattctct accgacccca cgcaggaatt
      901 gacggtgtta gtaaacaatg atgggattta aaggatgaag ataaataagt tttacgtgtt
      961 tatctgcacc gtgttagtaa acaatgatgg gatttaaaga aaagtgggcg attgggtttc
     1021 ttgcgttttt agaattgtgt tagtaaacaa tgatgggatt taaaggtaat taatataatc
     1081 aataataata attctgaaat aagtgttagt aaacaatgat gggatttaaa gacaagttaa
     1141 gcaaagaaaa agagcttatg cgaattaagt gttagtaaac aatgatggga tttaaagcca
     1201 tcgattttat tgaaaacgct ctgggtcgcg tagcgtgtta gtaaacaatg atgggattta
     1261 aagcagggaa tgagcatcag gaatattgtt gattatatgt gttagtaaac aatgatggga
     1321 tttaaaggaa gtctttttga tgctgctaaa agatttaaaa ggggtgttag taaacaatga
     1381 tgggatttaa agaagggatt tttgcgcgga aattatggca gttatagcag tgttagtaaa
     1441 caatgatggg atttaaagcg gcttcagcgg ctttaaccac tctgggtttt tctggtgtta
     1501 gtaaacaatg atgggattta aagattgtgt ttgatgttat tcttttaagg ttttctgaag
     1561 tgttagtaaa caatgatggg atttaaagtg ggttttatcg cttttttctt ttgcggaaaa
     1621 agcggtgtta gtaaacaatg atgggattta aagatatctt ttttaaagaa acttaccgcc
     1681 gccgctcgcc gtgttagtaa acaatgatgg gatttaaagc tgctgccact gcctaaatgc
     1741 gtgtcaagta ttcgcgtgtt agtaaacaat gatgggattt aaagagagca ttcacagcga
     1801 atttaaagcc ggctgcgcga tgtgttagta aacaatgatg ggatttaaag cgtttaatag
     1861 tatcatatat ccgtattcgg ataattggtg ttagtaaaca atgatgaggg actacccccc
     1921 ggaagttgtg ccagaagtaa ttagagtgta aaataaa
//
LOCUS       DAOPCD010000176         1918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_47125_length_1918_cov_3.170692, whole genome shotgun sequence.
ACCESSION   DAOPCD010000176 DAOPCD010000000
VERSION     DAOPCD010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47125_length_1918_cov_3.170692"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLQ55_04345"
     CDS             <1..598
                     /locus_tag="PLQ55_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HPK29193.1"
                     /translation="SKIGPFVIVSESSIASGVRRIVALTGLAAYEHLKTYSDTVKRIS
                     KELGLNDKKDVVPHVRTLVEKNALLNSEVIALKTQELHRIASALTPKKHGDTNIYLEY
                     FTTSNREDLGVIADKVRSTDECALLVLVGNNTNDFPLLISVSKELSKSLNANKVMRAL
                     TSAFGGRGGGRPDFANGVLLNLPEKTKALATILKVLDE"
     gene            591..1025
                     /gene="ruvX"
                     /locus_tag="PLQ55_04350"
     CDS             591..1025
                     /gene="ruvX"
                     /locus_tag="PLQ55_04350"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPK29194.1"
                     /translation="MSKILGLDLGTKTLGIAISNVDATFAVAYENYVFPPHYYKKARA
                     HVLAICAKENISEIALGYPLNTDGTVGERAKSAERFRDDLLREGSPPIVLVNETYTTL
                     AATEELERLGYKKEQIKAQIDAVAAKYILETYLERKRSNERK"
     gene            1012..1260
                     /locus_tag="PLQ55_04355"
     CDS             1012..1260
                     /locus_tag="PLQ55_04355"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HPK29195.1"
                     /translation="MKENDVIVITTEEGGEEEFIILFTYENEERGTKYVFFYDENNDE
                     EVMCAKYFDDGTLEYVEDEEELAEMEEVFRAYESELEE"
     gene            1340..1819
                     /gene="greA"
                     /locus_tag="PLQ55_04360"
     CDS             1340..1819
                     /gene="greA"
                     /locus_tag="PLQ55_04360"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPK29196.1"
                     /translation="MTNKYTLTQEGVDALEEELSVLVNVERPLVLKEIEEARSFGDLS
                     ENADYDAARNKQANIESRIMEIEHILAHKEIVEYSKTSKTVAVGNYVEVHDRTNDDTF
                     SLRILGQFEGKPLNGTVSVDAPIAQAILGKKVGDRVNVKAQPPYEVEILNISTEPIE"
BASE COUNT          651 a          316 c          422 g          529 t
ORIGIN      
        1 aagtaaaatc ggtccgtttg tgatcgttag tgaaagtagt atcgctagtg gggtgcgtcg
       61 gattgttgct ttaaccggat tagcggccta cgaacaccta aaaacatatt ccgacactgt
      121 gaaaagaata agtaaagagc ttgggcttaa tgataaaaaa gatgtcgtgc cacatgtgcg
      181 cactctagta gaaaaaaatg cccttttaaa tagtgaagtt atagcgctta aaactcaaga
      241 acttcatcgc atcgcttccg ctctaacacc gaaaaagcac ggtgatacga atatatattt
      301 agaatatttc actacttcaa atcgtgaaga cttaggagtt attgccgata aagtgagaag
      361 cactgatgag tgcgctttac ttgtgttagt tggtaataat acaaatgact tcccactgct
      421 tattagtgtg agtaaagagt tatcaaagtc gctaaacgct aataaagtaa tgcgcgcatt
      481 aacaagtgct tttggtggac gcggtggcgg ccgacctgac tttgctaacg gtgtgctctt
      541 aaacttacca gaaaaaacaa aagcattagc gacaatatta aaggtgcttg atgagtaaaa
      601 tattaggctt agacttagga actaaaacgc ttgggatagc gataagtaat gttgacgcga
      661 catttgcagt tgcttatgaa aattatgtgt ttcccccgca ttactataaa aaggcgcgtg
      721 cccatgtctt agcaatatgc gctaaagaaa acattagtga aattgcgctt ggctacccgc
      781 ttaacactga tggcactgtt ggggaaagag caaagtctgc ggaaagattc cgtgatgatc
      841 tcttgcgtga aggttcgccg cctattgtat tagttaatga aacttacacg acgcttgcgg
      901 ctaccgaaga acttgaacga ctcggttata aaaaggagca aattaaagca cagattgacg
      961 ctgtcgcggc aaaatacata ttagaaactt atttagaaag gaaaagaagc aatgaaagaa
     1021 aatgatgtga ttgttattac aaccgaagaa ggcggagaag aagagtttat tattcttttt
     1081 acgtatgaaa atgaagaacg aggcacaaaa tatgtctttt tctatgatga aaataatgat
     1141 gaagaagtga tgtgcgcgaa gtattttgat gacgggacat tagaatatgt tgaagacgaa
     1201 gaagaacttg cggaaatgga agaagtattc cgcgcctacg aaagcgaatt agaagagtaa
     1261 ccgcttccct tttgtttttg gttgagaatt ataagcatta tgttataatg cattagcaat
     1321 ttatatagga gaaatgacta tgactaataa atatacttta acccaagaag gggtcgacgc
     1381 cttagaagaa gaattaagtg ttctcgtaaa tgtagaacgg cctctagtgc tcaaagaaat
     1441 cgaagaagcc cgtagttttg gtgacttaag tgaaaacgca gattacgacg ctgcccgtaa
     1501 taaacaagcc aatatcgaaa gccggattat ggaaattgaa cacattttag cgcataaaga
     1561 aatcgttgaa tattccaaaa cgagtaaaac agttgccgtt gggaactatg ttgaagttca
     1621 tgaccgcaca aatgatgata ctttctcgct ccgtatttta ggacaatttg aaggtaaacc
     1681 acttaatggt acagtttctg ttgatgcccc gattgcccaa gccattttag gaaaaaaagt
     1741 tggtgaccgc gtaaacgtaa aagcccaacc tccctatgaa gtggaaattt taaatatttc
     1801 gactgaacca atcgaataat taatattttt gtagtaaaaa gataaataac ttcctactta
     1861 ttagtaggag gttttttatt tatgttaaag ctaatgattg gtgtagtgat cgctacag
//
LOCUS       DAOPCD010000177         1902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_47720_length_1902_cov_2.356253, whole genome shotgun sequence.
ACCESSION   DAOPCD010000177 DAOPCD010000000
VERSION     DAOPCD010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47720_length_1902_cov_2.356253"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="PLQ55_04365"
     CDS             <1..1031
                     /locus_tag="PLQ55_04365"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPK29197.1"
                     /translation="GNFGRTRGAGSDFHSLWPSDVKANQQAHNNNNYGVPTSNITNYQ
                     SDHDTYVGRNGNIEGYTSKVFEPLDEYKGDIARAMFYMPARYYEYIDKLHPKLELVNG
                     SPAAREASATLTGQAGCLETLLQWHEEDPVSEYEIRRNNLIANNYQGNRNPFIDYPQW
                     ARIAYDEGYIGTGATNALGLSSAAGLRDPFEDVTLKSISIDTTNVKKEYALNEGYDPT
                     GLVVTAHYENGGKRKVADFTTSMAKGEPLTKGGTKTITVSVTHEGVTKTASYNIEVKE
                     PLPIYVYIGVAAVVLLIIIIYIFSAKSKKAKKKRASNVKKVIKKATKSSSSTKSNSKR
                     KTTKTKKK"
     gene            1152..>1902
                     /locus_tag="PLQ55_04370"
     CDS             1152..>1902
                     /locus_tag="PLQ55_04370"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPK29198.1"
                     /translation="MKFNKLISVSILALLISGTSLTTTKQKEKVSAATPMPTNINMTN
                     RTDAEVSAYYEGVNGKSGDALLAFLYTKIKNHNEYNYESVTHRTIYKIIDRNWALDAL
                     DPNGPANTSNFNYSTDNGFIRKLYADYNDNIATADRFKNDGASRVSFDKEHIWAQSLG
                     NFGRTRGAGSDFHSLWPADVKGNQDAHSNYNFAVPKTSITTYKNDYGTYVGRNGYISG
                     YSQKVFEPLDQYKGDIARAMFYMPARYYEYID"
BASE COUNT          666 a          307 c          366 g          563 t
ORIGIN      
        1 ttggtaactt tggtcgcact cgtggagcag gtagtgattt tcattcatta tggccaagtg
       61 atgttaaggc taatcaacaa gcgcataata ataataatta tggggttcca acctctaata
      121 ttactaatta tcaaagtgac cacgatactt atgttggccg caacggaaat attgaaggtt
      181 atacatcaaa agtgtttgaa ccacttgatg aatataaagg tgatattgct cgcgctatgt
      241 tttatatgcc agcgcgttat tatgaatata ttgataaact acatccaaaa ctagaactag
      301 taaacggttc tcctgctgca agagaagcta gtgcgacact aaccggccaa gctggttgtc
      361 ttgaaacatt actgcaatgg cacgaagaag atcctgttag tgaatatgaa attagaagaa
      421 acaacttaat cgctaataac tatcaaggta acagaaatcc gtttattgat tacccgcaat
      481 gggcacggat tgcttatgat gaaggttaca ttggcacagg agcgacaaac gcattaggac
      541 tttctagtgc tgcgggttta cgcgatcctt ttgaagatgt taccttaaag agtatttcaa
      601 ttgatacgac aaatgttaaa aaagaatatg cattaaatga aggttatgac ccaacaggac
      661 tagttgtaac cgctcattat gaaaatggtg gcaaacgaaa agttgcagat ttcactacta
      721 gtatggctaa aggcgaacct ttaaccaaag gtggaacaaa aaccataact gttagtgtca
      781 ctcatgaagg ggtaacaaag actgcaagtt ataatattga agtcaaagaa ccactaccaa
      841 tctatgttta tatcggtgta gcagcagtag ttcttttaat aattatcatt tatatcttta
      901 gcgcgaagtc taaaaaagca aagaaaaagc gtgcttctaa tgttaaaaaa gttattaaaa
      961 aagcgaccaa atcaagctct tcaacgaaga gtaatagtaa aagaaaaaca acaaaaacaa
     1021 agaaaaaata actaataaca tttatcttaa attaggcgta ttttacgaca aaacacgcct
     1081 tttttattat ttatggactt ttttaatgtt agtgctataa tcattattac aacataaggt
     1141 agatagttat tatgaaattc aataaattaa ttagtgtttc aatccttgca ctattaataa
     1201 gcggaacttc gcttactaca actaaacaaa aagaaaaagt ttcagcggct accccgatgc
     1261 cgacgaatat taatatgact aataggactg acgccgaagt tagtgcttat tacgaagggg
     1321 tgaacggtaa aagtggtgat gcacttttag cctttcttta tacgaaaatt aaaaatcata
     1381 atgaatacaa ttatgaaagt gtaacgcata gaacaattta taaaattatt gatcggaatt
     1441 gggctttaga tgcacttgat ccaaacggcc ctgcgaatac atctaatttt aactatagta
     1501 ctgataacgg ctttattcgt aagttatacg ctgattataa tgataatatt gctactgcgg
     1561 atcggttcaa aaacgacgga gcgagtcgtg ttagttttga taaagagcat atttgggcgc
     1621 aatcacttgg taactttggt cgcactcgtg gagcaggtag tgattttcat tcattatggc
     1681 ctgctgatgt taaaggtaat caagatgcac atagcaacta taattttgcg gttccaaaaa
     1741 caagtattac aacttataag aatgactacg gcacttatgt cggtcgtaat ggttatataa
     1801 gtggttactc gcaaaaagta tttgaaccgt tagatcaata taagggtgat atcgctcgtg
     1861 ctatgtttta tatgcccgcg cgttattatg aatatattga cg
//
LOCUS       DAOPCD010000178         1884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_48386_length_1884_cov_2.272280, whole genome shotgun sequence.
ACCESSION   DAOPCD010000178 DAOPCD010000000
VERSION     DAOPCD010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48386_length_1884_cov_2.272280"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLQ55_04375"
     CDS             complement(<1..255)
                     /locus_tag="PLQ55_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29199.1"
                     /translation="MKRKKQVFRASLTFIALSFLLIGSLLNNKEDYFYRLKGETTYSC
                     ALPAGYVEINTIASTALTLTSHGGGNDYHNKVNTDTFRGTV"
     gene            complement(275..919)
                     /locus_tag="PLQ55_04380"
     CDS             complement(275..919)
                     /locus_tag="PLQ55_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29200.1"
                     /translation="MKTLNEGESNTIKKPTSALEKALVILLVVFLGATITMLVFYHFA
                     RWGLGLNIFVFLLILIVPALLLLGVIIIIVNIAGKRNFEISWKRPLLPTFIVLGAGVF
                     SLLFVIGERATFKIRYTFTIEKWAKATPDERSVLVDSFLEQYDLNTFNDAMVIEYLGE
                     PDFKETLEVGEPPSFAGYYYIYNLGFVRDFIDPTFFDIKTDASGKVVYYNVRYT"
     gene            complement(1083..1214)
                     /locus_tag="PLQ55_04385"
     CDS             complement(1083..1214)
                     /locus_tag="PLQ55_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29201.1"
                     /translation="MFDKPILYSELRKKFTCRTKMAEYLLARTNALLYTPLPVAPSV"
     gene            complement(1181..>1884)
                     /locus_tag="PLQ55_04390"
     CDS             complement(1181..>1884)
                     /locus_tag="PLQ55_04390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPK29202.1"
                     /translation="RFFPVYYFVIPSLLGTLAQGLFYGISTGWMLTSIALLFVQLHIA
                     NQNAYEDDLSGLYNRKYFGWMVEKLTRSKNNAFIGAIMMDIDCFKSINDKFGHSVGDD
                     AIRSFGWILTNITTATTIAFRVGGDEFIVLHIGGTEEDLAKIKDAINEQLDEFNETEG
                     KPYTLAVSRGLQLVTQMIQALTFSFINLKNFIQALFFLRKWHYGAGLTFRFTQLISAR
                     RRMSLCLINQFFILN"
BASE COUNT          658 a          357 c          292 g          577 t
ORIGIN      
        1 aactgtacct cgaaaagtat cagtgtttac tttattatga taatcattac cgccaccgtg
       61 actagtaagc gttaaagcag tgctcgcaat agtgttaatt tcaacatatc ccgcagggag
      121 agcacatgaa taagttgttt cacctttaag tctataaaaa taatcttctt tattattaag
      181 taatgaccca ataagcaaaa aagaaagagc aataaaagtt aaggaagctc taaacacttg
      241 cttttttcgt ttcataaata gtacttctcc taatttacgt atatctaaca ttgtaataaa
      301 caactttacc actagcgtca gttttaatat caaaaaatgt cggatcaata aaatcacgca
      361 caaaaccaag attataaata taataataac cggcaaatga agggggttca ccaacttcaa
      421 gtgtttcttt aaagtcaggc tcacctaaat attcaataac cattgcatca ttaaatgtat
      481 ttaaatcgta ttgttcaaga aaagaatcaa ctagaacact acgttcatcg ggtgttgctt
      541 ttgcccactt ctcaatcgta aaagtgtacc ggattttaaa agtagcccgt tctccaatca
      601 cgaataaaag tgaaaatact cccgcaccaa gtacaataaa tgttggcaat agtggtcttt
      661 tccacgatat ttcaaaattt ctttttccag caatattgac gataataatg atgacaccaa
      721 gcagtaaaag cgctggtaca attaatatta ataaaaatac aaaaatatta agccctaacc
      781 cccagcgcgc aaagtgataa aagacgagca ttgttatagt cgcgcctaaa aaaaccacaa
      841 gtaaaataac taaggctttt tcaagtgcac tagtgggctt ttttatcgtg ttactttctc
      901 cttcattcaa agttttcata atcaaattat agcaaattaa taaatttgca cttttgctaa
      961 taatattttc ataacaaaga ctttttaatt aacttaacat aaaattcaat gaacaagtta
     1021 attgtttctt tcgtttatga attttactta atcatgattt agttaattat gatttacttt
     1081 ttttaaacac tcggtgcaac aggaagcgga gtgtagagca aagcatttgt tcttgctaaa
     1141 agatattctg ccattttagt gcgacatgta aacttttttc ttaattcaga ataaagaatt
     1201 ggtttatcaa acataaagac attcgtcttc tcgcggagat aagctgcgta aatcggaatg
     1261 tcaatcccgc gccgtaatgc catttccgca aaaagaacaa agcctggata aaattcttta
     1321 agttgatgaa agaaaaagtc aaggcttgta tcatttgtgt aacaagttga agcccccttg
     1381 aaaccgctaa ggtgtaaggt tttccttcgg tttcattaaa ttcatctagt tgttcattaa
     1441 tcgcgtcttt tatcttggct aagtcttctt cagtaccgcc gatatgcagc acaataaatt
     1501 cgtcaccacc aacacggaaa gcaattgtcg tcgccgtcgt aatatttgtt aaaatccagc
     1561 caaaactgcg gatggcatca tctccaaccg aatgaccgaa tttatcgtta attgatttaa
     1621 aacaatcaat atccatcata attgccccaa taaaggcatt gtttttacta cgcgtaagtt
     1681 tttctaccat ccatccaaaa tatttccgat tatataatcc cgagagatca tcttcataag
     1741 cattttggtt agcgatatgc agttgcacga ataaaagtgc aatacttgtg agcatccacc
     1801 ccgttgaaat accgtaaaag agaccttgcg caagtgtacc taaaagggac ggaatcacaa
     1861 agtaatacac agggaagaaa cgaa
//
LOCUS       DAOPCD010000179         1877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_48635_length_1877_cov_2.797475, whole genome shotgun sequence.
ACCESSION   DAOPCD010000179 DAOPCD010000000
VERSION     DAOPCD010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48635_length_1877_cov_2.797475"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1128
                     /gene="trpS"
                     /locus_tag="PLQ55_04395"
     CDS             148..1128
                     /gene="trpS"
                     /locus_tag="PLQ55_04395"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPK29203.1"
                     /translation="MMRVLSGIKPTGKLTLGNYIGALKNFSQYQSDHEVFIFIADLHA
                     LTLPIDPDELRNNTKLLAAFYLAAGLDPNKVTIFKQSDVSAHAELNAILQNYLYMGEL
                     SRMTQFKEKSQSMDHSGIGLGLFAYPVLMASDILLYEANIVPVGEDQIQHIEITRDLV
                     VRFNNRYGKVLVMPEPQVTRVGKRIMSLSDPSVKMSKSESGKGDIYLTDDLKTMRKKI
                     MSAVTDSGSEIYVDEENKPGITNLLTIYAALTDKSLESCAAQFKDYRYGDFKKEVANV
                     VIDTLGPFQAKAMSFLESGEVDRVLSEGAKKATVIANKTLVKVKKAVGLL"
     gene            1125..>1877
                     /locus_tag="PLQ55_04400"
     CDS             1125..>1877
                     /locus_tag="PLQ55_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HPK29204.1"
                     /translation="MNKDKYLIAIDLDGTLLTDDKKITPKTRNYLNALHDEGHLIVIA
                     TGRPFRAAYGFQQQLGVNGPLVTYNGSLTSNPHDASFLTRTRKFDRFALKDMVDAIGF
                     DALDNLMIETEHVIYLLHEDEALNNFFWNDRERIIYGNPFLHLNEDPLALIFLFKNTD
                     PEYKKEVSRIVESFPDLHIRFWNLEGYAELWIGESTKKDGLEHIANFYNFPQKRIIAF
                     GDASNDKEMLAWANYGVWMKNGVPSVEAYANMK"
BASE COUNT          643 a          331 c          346 g          557 t
ORIGIN      
        1 acataaaatc attaaaacac ttaatcttgt taattttaat tattcatcta tccacctgaa
       61 atgaatttta attaattttt gacaatgtat gcgcttagtt gactataaaa ataatataga
      121 ttataatttt taagattaaa aggagtaatt atgcgagtat taagtggaat taaaccaacc
      181 ggaaaactaa cacttggtaa ctacattggg gcgttaaaaa acttctccca atatcaaagt
      241 gaccatgaag ttttcatatt cattgccgac ttacacgcct taacgctgcc gatagaccct
      301 gatgagttac gcaataatac aaagttatta gcagccttct atctagcagc cgggcttgat
      361 cccaataaag taacaatatt taaacaaagt gatgttagtg ctcacgctga attaaatgcg
      421 atcctacaaa actatttata tatgggtgaa cttagtcgta tgactcaatt taaagaaaaa
      481 agtcaatcaa tggatcatag cggtattggc ttaggtttat ttgcctaccc cgtcttaatg
      541 gctagcgata ttttacttta tgaggctaat atcgtgccgg tcggcgaaga ccaaattcaa
      601 catattgaaa ttacacgcga cctagttgtg cgctttaata accggtacgg aaaagtttta
      661 gtaatgcccg agccacaagt aacgcgtgtc ggtaaacgca ttatgtcact aagtgacccg
      721 agtgttaaaa tgagcaaaag cgaaagcggt aaaggtgata tatatctaac tgatgactta
      781 aaaacaatgc gcaagaaaat tatgagcgct gttactgatt ccggtagtga aatttatgtt
      841 gatgaagaaa ataaacctgg gattacaaat ttacttacaa tttacgctgc gctaactgat
      901 aaaagccttg aatcatgcgc tgcgcaattt aaagattacc gttatggcga ctttaaaaaa
      961 gaagtcgcta atgttgttat tgatacactt ggtcctttcc aagcaaaagc aatgtctttt
     1021 cttgaaagcg gggaagttga tcgcgtccta agtgaaggag caaaaaaagc aacagttatc
     1081 gctaataaaa cattagtgaa agtaaagaaa gcagtaggac ttttatgaat aaagacaaat
     1141 atttaattgc cattgatctc gatggcacac tcttaactga tgataaaaaa atcaccccta
     1201 aaactcgtaa ctatctaaat gcccttcacg atgaaggaca tttaattgtc atcgccaccg
     1261 ggcgaccatt tcgcgcagca tatggttttc aacaacaatt aggagtaaac gggccacttg
     1321 ttacatataa cggctcttta acatctaatc cacatgatgc ttcattccta acacgcacac
     1381 ggaaatttga tcgctttgcg cttaaagata tggtagacgc tatcgggttt gatgccttag
     1441 ataatttaat gattgaaact gaacatgtta tttatttatt acatgaagat gaagccctta
     1501 ataatttctt ctggaatgat cgtgaacgca taatttatgg taaccccttc ttacatttaa
     1561 acgaagaccc tttagcgctt atattcttat ttaaaaatac tgatcctgaa tataaaaagg
     1621 aagtatcgcg tattgtcgaa tcattccctg atttacatat tcgtttctgg aatttagaag
     1681 gttatgcaga attatggatt ggcgaaagca caaaaaaaga cgggcttgaa cacattgcta
     1741 acttttataa tttcccacaa aagcgcatca ttgcttttgg cgacgcatct aatgataaag
     1801 aaatgctagc gtgggcaaat tatggtgtgt ggatgaaaaa cggtgtacca agtgttgaag
     1861 cttacgcaaa tatgaaa
//
LOCUS       DAOPCD010000180         1870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_48891_length_1870_cov_2.558127, whole genome shotgun sequence.
ACCESSION   DAOPCD010000180 DAOPCD010000000
VERSION     DAOPCD010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48891_length_1870_cov_2.558127"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /gene="ruvA"
                     /locus_tag="PLQ55_04405"
     CDS             complement(<1..520)
                     /gene="ruvA"
                     /locus_tag="PLQ55_04405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPK29205.1"
                     /translation="MIYGLKGIVTKQTATTIVLDVRGVYYEVKVPSVPQQQQFSELFL
                     YTYQVIREDEHYLVGFLSLEEKSLFTELLNVSGIGPKTALGIMTATTPERFLQAVTSE
                     ELSYLKKLPGVGPKGASQIILDLKGKLQTDTSKGLNPYEQEVTLGLQSLGFKKSEITK
                     VLKKVSNKSLDAN"
     gene            complement(618..1346)
                     /gene="nadE"
                     /locus_tag="PLQ55_04410"
     CDS             complement(618..1346)
                     /gene="nadE"
                     /locus_tag="PLQ55_04410"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPK29206.1"
                     /translation="MGQESLQNYLQYLVRYLKRRARGFNGYILGLSGGLDSAVVALLA
                     QKAVKDDLLCVIIDIESEKCDMDDALELCKLHNLNYYYIDLTAEYRAVVKKLEESKKL
                     TELSKINTKVRLRMVTLYALGQTHSKLVLGTDNKAELFTGYFTKHGDGAADLFVLSSL
                     TKGEVFAAARLLGVTPNIINKIPSAGLYVGQTDEAEMGIKYEDLDAFISGKEVSPSVK
                     ARAIELHRISNHKRRPIPRPREKR"
     gene            complement(1356..>1870)
                     /gene="cgtA"
                     /locus_tag="PLQ55_04415"
     CDS             complement(1356..>1870)
                     /gene="cgtA"
                     /locus_tag="PLQ55_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HPK29207.1"
                     /translation="LLRNEVKEYGYKLDERPFFVAVSKDETVDTHKKVKALAKKLKDI
                     KVLGFSSILHENIDTLINTMEEYIVNAPEIEMVDEEGVAVKYREYDLTATIDPDLFTI
                     EKVRAGVYRIVGEKVEERYHKYILNTEEAILSLLNYLREIGVEDALDKMGVNDGDTVI
                     LVDFEFEYFR"
BASE COUNT          538 a          416 c          290 g          626 t
ORIGIN      
        1 cattagcgtc aagcgactta ttactaactt tctttaaaac tttagtaatt tcacttttct
       61 taaaccctaa gctttgtaat ccaagcgtta cttcttgttc ataaggattt aagccttttg
      121 aagtgtcagt ttgtaattta ccctttaagt cgagaataat ttgactcgcg ccttttggac
      181 caacgcccgg taatttcttt aagtaactaa gttcttcact cgtgactgcc tgtaagaagc
      241 gctctggtgt tgtggccgtc atgatcccta aagcagtctt tggaccaatg cctgaaacat
      301 ttaaaagctc ggtaaataag cttttttctt caagacttaa aaagccaaca agataatgct
      361 catcttctct aattacttga tatgtatata aaaatagttc actaaactgt tgttgctgtg
      421 gcacggacgg cactttaact tcgtaataga caccacgcac atctaagaca atcgttgtcg
      481 ccgtttgttt tgtaacaatg ccttttaatc cataaatcat actcccacca tccttataaa
      541 tataagtaac acgacgaata aaagtacggc agctaggcca ctaataatcg tgttactcac
      601 aaatttttgc tttttcacta tcttttctcc cgtggtcgag ggattggacg tcgtttgtga
      661 ttgctgatgc ggtgcagttc tattgcgcgt gctttaacgc ttggcgatac ttctttaccg
      721 ctgataaagg cgtcaagatc ttcgtattta atgcccatct cggcttcgtc agtctgtccg
      781 acataaagtc cagcacttgg aattttatta ataatgtttg gcgtaacacc aagtaagcgc
      841 gctgcggcaa agacttcacc ctttgttaag cttgaaagga cgaataaatc agcagcccca
      901 tccccgtgct ttgtaaagta accagtaaag agttccgcct tattatcagt gccgagcact
      961 aatttgctat gtgtttgccc taaagcataa agcgtcacca tacgtaaacg aactttcgta
     1021 ttaattttac taagttcggt taacttttta ctttcttcaa gttttttcac aaccgcgcgg
     1081 tattctgcag ttaaatcaat gtaatagtaa tttaaattat gtagtttaca aagttctaag
     1141 gcatcatcca tatcacactt ttcgctttca atatcgataa tgacgcataa taagtcgtcc
     1201 tttacggctt tttgggcaag aagtgccaca actgcactat cgagaccacc acttaaccct
     1261 aaaatataac cattaaatcc gcgtgctcta cgctttaagt aacgcactaa gtactgtaaa
     1321 taattttgta aactttcttg tcccataatt cctccctagc ggaaatactc aaattcaaag
     1381 tcaacgagaa tcacagtatc accatcatta actcccattt tatcaagggc atcttcgaca
     1441 ccaatttcac ggagataatt taaaagtgat aaaatcgctt cttctgtatt taagatgtat
     1501 ttatgatatc tttcctcaac tttttcccca acaatacggt acactccggc tctaactttc
     1561 tcaatcgtaa ataaatcagg gtcaatagtc gccgttaagt cgtactctcg atacttaacc
     1621 gcaacgcctt cttcatcaac catttcaatt tcgggagcgt taacaatata ttcttccatc
     1681 gtattaatta aagtatcgat attttcatgt aagatactac taaaaccaag cactttaata
     1741 tcttttagtt ttttcgctag tgcttttact tttttatgag tatctactgt ttcatcttta
     1801 cttacagcca caaagaaggg tctttcatct aatttatacc catattcctt cacttcatta
     1861 cgcagtaact
//
LOCUS       DAOPCD010000181         1858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_49325_length_1858_cov_3.995563, whole genome shotgun sequence.
ACCESSION   DAOPCD010000181 DAOPCD010000000
VERSION     DAOPCD010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49325_length_1858_cov_3.995563"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PLQ55_04420"
     CDS             complement(<1..308)
                     /locus_tag="PLQ55_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPK29208.1"
                     /translation="MYRTIENGNINEKLVGQTVKIIGWVAKKRDLGGLTFVDLRDRSG
                     IVQVLINEGVARPDIRNEYIIQIEGVVTLKEVPNKNLKTGKVEVVASKVTLINKAKNP
                     P"
     gene            complement(313..>1858)
                     /gene="hisS"
                     /locus_tag="PLQ55_04425"
     CDS             complement(313..>1858)
                     /gene="hisS"
                     /locus_tag="PLQ55_04425"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPK29209.1"
                     /translation="PKGTHDLIGNEDRAYAYISDTCGIVARLYGFDPLTTPIFEANEL
                     FARGVGDATDIVRKEMYVFTDKGNRLMALRPEGTAGVMRSIIANKLYATNDLPLRYYY
                     SGPMFRYERPQLGRYRQFHQFGVESVGSTSIIADLEVILLAIHSLQALGLENITTKIN
                     SLGKQEARDAYREALKKYYEPHLKDVCPDCKVRYEQNPLRMLDCKVPSDVELAKAAPK
                     VTDFLTEEDKKDLEFIKTSLEEYGVNVEVDYGLVRGLDYYSGIIFEFVYIPPSGKDYG
                     AIGGGGRYDKLVEELGGPSLEGVGFAFGLERLYHILNEEGKLEHTAVKDDFILVAIGE
                     EARTSGYSLLTALRANGLRGIMNFEDKSFKSLFKIAENRGARVALILGEAEIKNEVVS
                     VRNLETQEQVEVPFAEVINYILDLCEEYHACNCGCDHEDCDCDNHDHKCKCGCEHEDC
                     DCDHEECECDSHKENGECCGEHHHDEKHECCRKHEHEKNHECTGKGKGRGVNCKKHEE
                     KEEEKA"
BASE COUNT          505 a          433 c          315 g          605 t
ORIGIN      
        1 ggcgggttct tagctttatt aataagtgtg actttacttg ccacgacttc aactttccct
       61 gtctttaagt ttttatttgg cacttcttta agcgtgacaa cgccttcgat ttgaataatg
      121 tattcattgc gaatatcagg gcgcgctacg ccttcgttta ttaacacttg gacaatcccg
      181 cttcggtcac gtaaatccac aaatgttaag ccgcctaaat cacgtttttt agcgacccaa
      241 ccaatgattt taactgtttg tccaactagt ttctcattaa tatttccgtt ttcaattgtt
      301 cgatacataa gtttatgcct tctcttcttc tttttcttca tgttttttgc agttcacacc
      361 acgaccttta cctttgccag tgcattcatg atttttttca tgttcatgtt tacggcagca
      421 ttcatgcttt tcatcgtgat gatgttcgcc gcagcattca ccgttttctt tatgagagtc
      481 gcattcacac tcttcatggt cacagtcgca atcctcatgt tcacagccgc atttacattt
      541 atgatcatga ttatcacaat cgcaatcttc atggtcacac ccacaattac aggcgtgata
      601 ttcttcacaa aggtcaagta tataattaat cacttcggca aacggtactt caacttgctc
      661 ttgtgtttct aagttccgta cactgacaac ttcattttta atttcagctt cgccgagaat
      721 taaagcaaca cgtgctccac gattttcggc gattttaaag agtgatttaa atgatttatc
      781 ttcaaaattc atgattccgc gtagcccgtt cgcgcgtaaa gcagttagta aactataacc
      841 actagtccgt gcttcttcac cgatcgcgac aagaataaag tcatctttaa ctgcggtgtg
      901 ctcaagtttt ccttcttcat ttaaaatgtg atagagccgt tcaaggccaa aagcaaaacc
      961 aacaccttct aaacttggac cgcctaactc ttccacaagt ttatcatagc ggccaccgcc
     1021 accaatcgct ccgtaatctt tacctgaagg cgggatatag acaaattcaa agataatgcc
     1081 tgaataataa tcaagtccgc gcactaatcc gtaatcaact tccacattaa caccatattc
     1141 ttccagtgaa gttttaatga attcaaggtc ttttttatct tcttcagtta agaaatcagt
     1201 aacttttggt gccgctttgg caagttcgac gtcacttggt actttacaat caagcatccg
     1261 tagcggattt tgttcataac gcactttaca atcaggacat acatccttta aatgtggttc
     1321 ataatatttt ttaagtgctt cgcgataggc gtcacgcgct tcttgcttcc caagactatt
     1381 aattttagtt gtaatatttt caagtcctaa agcttgtaaa gaatggattg caagtaaaat
     1441 tacttcaaga tcagcaataa tgctcgtact accaacactt tcaaccccaa attgatggaa
     1501 ttgtcgataa cgcccaagct gtggtctttc atagcggaac attggtccac tgtagtaata
     1561 acgcagcggt aaatcattcg tagcgtaaag cttattagca ataatgctac gcattactcc
     1621 tgcggtacct tcaggacgca gtgccattaa gcggttacct ttatcggtaa atacatacat
     1681 ttctttccgt acaatgtctg tcgcatcacc gacaccacgg gcaaatagtt cattagcctc
     1741 gaaaatcggt gttgtcagtg gatcaaaacc ataaagtcgg gcaacgatgc cgcatgtatc
     1801 acttatataa gcataagcac ggtcttcgtt tccaattaag tcatgtgtgc ccttaggg
//
LOCUS       DAOPCD010000182         1857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_49373_length_1857_cov_3.556049, whole genome shotgun sequence.
ACCESSION   DAOPCD010000182 DAOPCD010000000
VERSION     DAOPCD010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49373_length_1857_cov_3.556049"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /gene="deoC"
                     /locus_tag="PLQ55_04430"
     CDS             complement(<1..582)
                     /gene="deoC"
                     /locus_tag="PLQ55_04430"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002963360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPK29210.1"
                     /translation="MKKNKLIDHTLLKADASPAQIEKLCQEALEYDFMSVCVNPGYVP
                     LASMLLAESDVRVCTVVGFPLGATSTEAKVLEAQIAVEEGADEVDMVINVGKLKAGLD
                     LEVFTEIRAIREALPDWVILKVIIETCLLTDDEKVRACLLSRKAGADFVKTSTGFSTG
                     GATVADVKLMRDTVGSDMGVKASGGVRTMEDLEA"
     gene            complement(802..1005)
                     /gene="rpmF"
                     /locus_tag="PLQ55_04435"
     CDS             complement(802..1005)
                     /gene="rpmF"
                     /locus_tag="PLQ55_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPK29211.1"
                     /translation="MAVQQRRGSKTRKRLRRTHFKLNVTGLTTCPNCGTLIRAHHVCP
                     ECHHYAGKDVITKEEPKKGKAKK"
     gene            complement(1144..>1857)
                     /locus_tag="PLQ55_04440"
     CDS             complement(1144..>1857)
                     /locus_tag="PLQ55_04440"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPK29212.1"
                     /translation="IVLTGIHTGAYGKDLGNIKFSNIVEMILEACPSLHKLRVSSIEA
                     NEIDDDFLRLMLKDKRIARHLHIPLQSGSDEVLRRMNRHYTTSEFIHKIENIREKVPN
                     IAITTDVIVGFPGESEKLFNETVAFIKKVNFTQLHVFPYSARTRTPAASFKEQVLGPV
                     KKERVRTLINLSEDLYRSYAQRFVGEKMQVLIETYDDKEEVYVGHTTNYLAVRVKSDS
                     DIKHEVITIIYDPNISKKI"
BASE COUNT          543 a          378 c          295 g          641 t
ORIGIN      
        1 cgcttcaaga tcttccatag tacggacgcc gccactagct ttaacgccca tatcactccc
       61 aacagtgtca cgcattaatt taacatcggc tactgtggct ccgccggtac taaaaccagt
      121 actagttttc acaaaatcag caccagcttt acggcttaaa agacacgctc taactttttc
      181 gtcatcagtt aataaacatg tttctataat aactttaaga atgacccaat caggaagtgc
      241 ttcacgaatc gcccggattt cagtaaagac ttctaaatct aagccagctt taagtttacc
      301 gacattaatt accatatcaa cttcgtcagc accttcttcg accgcaattt gcgcttcaag
      361 aactttcgct tcagtgcttg ttgcaccaag tgggaagcca acaacagtac acacacggac
      421 atcactttcc gctaaaagca tactcgcaag tggaacgtac cccggattta cgcatacact
      481 cataaaatcg tattcaagcg cttcttgaca aagtttttcg atttgtgcgg gtgaagcatc
      541 cgctttcagt aaagtatgat caattagttt attctttttc atattaccct ccaaatgata
      601 ttttaccatt ttagctatta tttaattact tttaatatat aaaacaccta aaacaactat
      661 ttttctactt tataagaagt tttgtaaaag gattttcaaa agtgctaaaa gttcaagagt
      721 tttgtaaatg gattttcaaa actttggatt atttaaaata aaaaaggaag tttccttcct
      781 catttattta gcggttaata cctatttctt tgctttgcct ttttttggtt cttctttagt
      841 aatcacgtct ttaccagcat agtggtgaca ttctggacaa acatgatgcg cccgaatgag
      901 tgttccacag tttgggcaag tggtaagtcc agtcacgttt aacttaaagt gagtccggcg
      961 taagcgtttg cgggttttac taccacgtcg ttgttgaaca gccataaact tatcctcctt
     1021 cttgtgacta gggttattat agcaaaagaa aaagtagtgt caagaaattt agacatttta
     1081 tagtatttat tttattatat tgattatatg ctcataattt agtgcattat ttttatacag
     1141 tgtctagatt tttttactaa tattcgggtc ataaataata gtaattactt catgcttaat
     1201 atcgctatcg cttttaactc taactgctaa ataattagtg gtatgcccaa catatacttc
     1261 ttctttatcg tcataagttt caattaaaac ttgcattttt tcgccgacaa atctttgtgc
     1321 ataacttcga tataagtctt cacttaagtt tattagtgta cgcacccgtt cttttttaac
     1381 tggaccaagc acttgctctt taaaactagc ggcaggcgtt ctcgtccgcg cactataggg
     1441 gaagacatgt agctgcgtga agtttacttt tttaataaac gcgactgttt cattaaataa
     1501 cttttcactt tctccaggaa agccaacgat aacatcagtg gtaatagcaa tattaggtac
     1561 tttttctcta atattttcga ttttatgtat gaattcactc gtggtataat gccgattcat
     1621 cctgcggagc acttcatcac ttccactttg cagcgggata tggagatggc gtgctatacg
     1681 cttgtctttt agcattaaac gaaggaaatc gtcatcaatt tcatttgctt caatacttga
     1741 tactcgtaat ttatgtaatg atggacaagc ttctaaaatc atctccacaa tattactaaa
     1801 tttaatattc cctaagtctt taccgtaggc tcctgtatgg attcccgtta aaacaat
//
LOCUS       DAOPCD010000183         1854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_49477_length_1854_cov_35.153419, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000183 DAOPCD010000000
VERSION     DAOPCD010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49477_length_1854_cov_35.153419"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1258)
                     /locus_tag="PLQ55_04445"
     CDS             complement(206..1258)
                     /locus_tag="PLQ55_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29213.1"
                     /translation="MLVDTLTIRLNDVVYHEPNLTTQAPSVDVRTGEVAPSLEICGYN
                     ASKAFLNTPHLQFTSKAKRLPTGDVIPLEAVKFSVPKVLERDEPCVNLDADDVRGALL
                     IVRDELENNGIFAELLDGSLWRVDATADVQTNAPFVAYTPIFESMHCARFHDANFGTT
                     FRIGGKEKQFCIYNKIAEFKMRGVDVTRMPENVARFEYRMLKAHACRKAGFGDVTALL
                     ENYDELRAQTLEAWNYVLERKELSKSAEAFSAFMQLAAHSGAMCEAGRSGTQSSIAEN
                     SGTKWIANAERALGLHYLLENGVTPNAYYKLLLELGASRKAASEHRKEMFAMQLVADG
                     VNLYDELKVKLNDAFT"
     gene            complement(1453..1650)
                     /locus_tag="PLQ55_04450"
     CDS             complement(1453..1650)
                     /locus_tag="PLQ55_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29214.1"
                     /translation="MLDVKRDERGRVMKRVPDEYSKRIYGFRVTEREYALIKRARASG
                     IEPREVMLKELRKLVENEKMQ"
BASE COUNT          516 a          451 c          299 g          588 t
ORIGIN      
        1 cttgtgttac caaacataat tcatcgctgt tatcccactg tattactgcg ttcatccata
       61 attccaagtg acatttggac atttacaaca ttcagctttt aaccagtttg taccagtaaa
      121 actggcacca aattcgcatt tctactaagt ggtggtgaat tatgcgtgct aacgtaaagc
      181 ttctaaaaag tagccaattt tcgctttacg tgaacgcatc gttaagctta actttcaatt
      241 catcatacaa attcacacca tcagctacta attgcatcgc gaacatttct ttacgatgtt
      301 cgcttgcagc tttgcgcgat gcaccaagtt ccaaaagaag cttgtaatat gcgtttggag
      361 ttacaccatt ttctaacaag taatgcaagc caagtgcacg ttcagcatta gctatccatt
      421 tcgttccgct attttccgct atgctacttt gcgtcccgct acgtcccgct tcgcacattg
      481 cgccgctatg cgccgctaac tgcataaaag cgctaaaagc ttcagcactt ttactaagtt
      541 cttttcgttc cagaacgtaa ttccacgctt ctaacgtttg tgcgcgtaat tcatcatagt
      601 tttccagcaa tgcagttaca tcaccaaaac cagcttttct acacgcgtgc gctttgagca
      661 ttctatattc aaagcgcgct acgttttctg gcattcgtgt aacatctaca ccgcgcattt
      721 tgaattcagc tattttgtta taaatgcaga attgcttttc ttttccacca atacgaaaag
      781 ttgtaccaaa attcgcatcg tgaaaacgcg cgcaatgcat actttcaaaa attggtgtgt
      841 aagctacaaa tggtgcgttc gtttgaacat cagcggtagc atccacgcgc cacaagctac
      901 catcaagcag ttcagcgaaa ataccattgt tttctaattc atcacgcaca attagcaacg
      961 caccacgcac atcatcagca tccaaattca cacacggttc atcgcgttct aacacttttg
     1021 gtacagaaaa cttcaccgct tctaatggta ttacatcacc agttggtaag cgctttgctt
     1081 ttgaagtgaa ttgtaagtgt ggagtgttca gaaatgcttt acttgcgtta tagccacaaa
     1141 tttctaagct tggtgcaact tcaccagtgc gcacatcaac acttggagct tgcgttgtta
     1201 agtttggttc gtggtaaacc acatcgttca agcgaatggt aagcgtatct actaacattg
     1261 cttaccacca caaaacgtag ctttgtaacc attttggtta ccaaaagccc aattgttata
     1321 cttggagctt ttggtaactt cactattgca caccaaaatt atgcgtgcta taattatgtt
     1381 aagaaatgga gcatatgaag cgttcatttc caacaccgct aaagcaacgc atccaacgcg
     1441 ttgcttttct tattactgca tcttttcatt ttccaccaac tttcgtaatt ctttaagcat
     1501 aacttcgcgt ggttcaattc cacttgcacg cgcacgtttt attaatgcat attcacgttc
     1561 cgtaacgcga aaaccataaa tgcgctttga atattcatct ggtacgcgct tcatcacgcg
     1621 accgcgttca tcacgcttca catccaacat aattcaccac cattccaact ttcatagcta
     1681 acaccaagta taacacggta attcaaaata agtaaatgct ttaccaattc atcaaagttc
     1741 atcaatagtt catctttttg ttacattgtt tgttcttttt acaacgcttg caattattac
     1801 ttgtgttacc aaacataatt catcgctgtt atcccactgt attactgcgt tcat
//
LOCUS       DAOPCD010000184         1844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_49902_length_1844_cov_3.321968, whole genome shotgun sequence.
ACCESSION   DAOPCD010000184 DAOPCD010000000
VERSION     DAOPCD010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49902_length_1844_cov_3.321968"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1716
                     /locus_tag="PLQ55_04455"
     CDS             <1..1716
                     /locus_tag="PLQ55_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPK29215.1"
                     /translation="DLTADESWNGSEENRKRFLGLIEEGTTYTAGGVTVKTGFDHIKE
                     LGINALQIIPFYDQANDEREPSFNWGYNPQNYDVLEGSYSSNPYDGLVRIREFKQVVK
                     AYAAEDIRIIMDVVYNHVANLGGHSFNKLVPGYYFRYNADGSPTSASGCGNDTASERI
                     MMENFMRDSTAFWTKEYKIGGFRFDLMGLHTVKSMNTVRNRLVEIRPDIFIIGEAWDM
                     GGGSVSANGANQNNIHKVPGVGAFNDKLRDSVKGDTNGKARGFVQQAASEIYQGTKNS
                     IKDGMLGKISGTQSSPVQTVNYVACHDNLALYDKLVNAGGHLFGLDQLAQQNIQAEAI
                     SLLSQGVSFIHAGSEILRSKPLGDGKFDHNSYQSPYSVNSIKWNEKVTNLNVFERYKE
                     LIAIKRDTPGFHYTTSAEISANVSVKFGSEIGYQDTLIKLVVVDGETTYTAYFLGAGT
                     RVRINDLEGQYVLFDSTNTMEKWSVLGKSVSLSSNATIVTVSGEGVTPPDSSEEPVTS
                     SSEEPSTPPATSTPDLPSSETLPSEEGEEANLTWLYITIPSLTVVGVGVGLILLFFRK
                     KKLSV"
BASE COUNT          568 a          361 c          390 g          525 t
ORIGIN      
        1 gaccttactg cggatgagag ttggaatggg agcgaagaaa atcgcaaacg cttccttggt
       61 ttaatcgaag aaggcacaac ttacaccgct ggtggtgtga cagtgaaaac cggctttgat
      121 cacattaaag aattaggaat taatgcccta caaatcattc cgttctacga tcaagctaat
      181 gatgaacgcg aaccatcctt taactggggt tataacccac aaaactatga tgttttagaa
      241 ggttcttact caagtaaccc gtatgacggt ctcgtgcgta ttcgtgaatt taaacaagtc
      301 gttaaagctt acgctgccga agatattcgt attatcatgg atgtcgttta taatcacgtt
      361 gcaaaccttg gtggtcactc ctttaataaa ttagtgcctg gctactattt ccgttataat
      421 gctgacggtt caccaacaag cgctagtgga tgcggtaatg acacggcaag tgaacgcatc
      481 atgatggaaa acttcatgcg tgattccact gctttctgga cgaaagaata taaaatcggt
      541 ggcttccgct ttgacttaat gggcttacac actgttaaat caatgaatac agtacgtaat
      601 cgtctcgtgg aaattcgtcc tgacatcttc attatcggtg aagcgtggga tatgggtggc
      661 ggtagtgtaa gtgctaatgg cgctaatcaa aataacattc ataaagttcc aggagtcgga
      721 gcatttaatg ataaattacg tgactctgtt aaaggtgata caaatggtaa agcacgcggt
      781 tttgtccaac aagccgctag tgaaatttat caaggaacaa aaaatagtat aaaagacggg
      841 atgcttggca agattagtgg cacacaaagt agtcctgtgc aaacagtaaa ctatgtggct
      901 tgtcacgata accttgcact atatgacaag ttagtcaacg ctggtggcca tttatttgga
      961 ctagaccaac tagcgcaaca aaatatccaa gctgaagcaa tttcactact ctcacaaggt
     1021 gtttccttta ttcacgctgg tagtgaaatc ttacgttcta aaccacttgg tgacggtaag
     1081 tttgatcata actcttacca aagcccgtat agtgttaata gcattaagtg gaatgaaaaa
     1141 gttactaatt taaatgtctt tgaacgctat aaagaattaa ttgccattaa acgtgatacc
     1201 cctggattcc actacaccac aagtgcggaa atcagcgcca atgttagtgt caaatttggt
     1261 tcagaaatcg ggtaccaaga tacacttatc aaactagtgg tcgttgatgg cgaaactact
     1321 tacactgctt acttcctagg tgcaggtacg cgtgttcgta taaacgattt agaaggacaa
     1381 tatgtactct ttgattcgac aaacacgatg gaaaaatggt cagtgctcgg taagagtgtt
     1441 tccttaagta gcaatgcgac aatcgtgact gtttctggcg aaggtgttac tccgcctgat
     1501 tcaagcgaag agccagttac ttcaagcagc gaagaaccaa gcactccgcc ggcaacaagt
     1561 actccggact taccaagtag tgaaacgctt ccaagtgaag aaggggaaga ggctaatctt
     1621 acttggctct atattactat cccatcactc accgttgttg gtgttggtgt tggcttaata
     1681 ttattattct tccgcaaaaa gaaattatca gtataattag ccgtttataa ctaatatttc
     1741 ggcaatttcc actcatttag gcaaatactt cgaatattat gcattataac tatgttatta
     1801 aacagcttta gcccttagca tattcttgac tgttaagggt gaaa
//
LOCUS       DAOPCD010000185         1838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_50146_length_1838_cov_2.844644, whole genome shotgun sequence.
ACCESSION   DAOPCD010000185 DAOPCD010000000
VERSION     DAOPCD010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50146_length_1838_cov_2.844644"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLQ55_04460"
     CDS             complement(<1..293)
                     /locus_tag="PLQ55_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HPK29216.1"
                     /translation="MTKRGLYVHIPFCTSLCHYCDFVKVIYQKKWVASYLEKLKHDLS
                     FFNVSRDLSTLYIGGGTPSSLSYYELRNLLELLKPYTANVKEYTVEANVESLT"
     gene            complement(286..537)
                     /locus_tag="PLQ55_04465"
     CDS             complement(286..537)
                     /locus_tag="PLQ55_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29217.1"
                     /translation="MIYLPTLAIDSPGLILLFLLLFFGLIALITFIIRRLIPGLKDKG
                     GRVNEQVAVQQELDRVLEPITDEKILAEFEKVDKETKND"
     gene            complement(548..>1838)
                     /locus_tag="PLQ55_04470"
     CDS             complement(548..>1838)
                     /locus_tag="PLQ55_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29218.1"
                     /translation="INDRLNQLDRRISRIQARIQEVYVDANHQKALKTLLTRDILKTI
                     AASYEINFHDGIVNQLINHNISVLNAWEMFLNDYYRGLELVAKSANLDFTEALLADVG
                     RLFNVPPLSNYIYRTTEIKTGRQVSVINHVHPHVPVNQISDAIADFYAFMATSTLPTI
                     VQSAAIIFYLDYIKPFEAHSEIIAILMAKAFLAERELALIAPYLNFELILERYRDARD
                     DVFAEVKKSADLTYFIDLLINVLNESLQTIEERIVFVVTHELEAEKHVLPPFEEETQE
                     ESVSATPPTDLFEHAQAEVAEKEETFKSEPVPVYTPKKSAIPLPPPREEEGVKVTPEE
                     ANLGITQLPPELSENDILRLERHIRESDPSLSRAQAYFYARHCTMGKYYSINDFKEVT
                     GCAYETARTSMEHLANSGYYRKEKFKNKFIYTPTRKA"
BASE COUNT          567 a          341 c          328 g          602 t
ORIGIN      
        1 aaggttaaac tttcgacatt agcctcgact gtatactctt ttacattagc cgtataaggc
       61 tttaataatt ctaaaagatt acgtaactca taatagctta aagacgaagg cgtcccgccc
      121 ccgatataaa gcgtacttaa gtcacgacta acattaaaaa aacttaagtc atgcttaagc
      181 ttttctaagt aagatgcaac ccactttttt tgataaatta ctttaacaaa atcgcaataa
      241 tggcatagtg aagtgcaaaa aggaatgtgt acataaagtc cacgtttagt catttttcgt
      301 ttctttatct actttttcaa attccgctaa gattttttca tcagtaattg gttcaagtac
      361 tctatctaat tcttgttgaa cagcgacttg ttcattaacg cgaccacctt tatcctttaa
      421 accaggaatt aagcggcgaa taataaaggt aataagcgca attaagccaa aaaatagtag
      481 taaaaataac agaattaatc ctggtgaatc aatcgcaagt gttggtaaat aaatcataat
      541 catcatccta tgctttccgt gttggtgtat aaataaattt gtttttaaat ttttctttgc
      601 ggtaatatcc gctgttagcg aggtgttcca ttgatgtccg cgctgtttca tacgcacagc
      661 ctgtcacttc tttaaagtca ttaattgagt aatatttacc catagtacag tgtctagcgt
      721 agaaataagc ttgagcgcga cttaaacttg gatcgctttc acgaatatgt cgctctaagc
      781 gtaatatatc attttcgcta agttctggtg gtaattgtgt aatccctaaa ttagcctctt
      841 caggtgtaac ttttacgcct tcttcttcgc gcggcggtgg aagcgggatt gcggattttt
      901 ttggtgtata aactggtact ggttcagatt taaaagtttc ttccttctcc gctacttcgg
      961 cttgggcatg ttcaaaaaga tcagttggtg gtgtagcact cacactttct tcttgcgttt
     1021 cttcttcaaa tggtgggagc acatgctttt ctgcttcaag ttcatgcgtc acgacaaaca
     1081 caatccgttc ttcaattgtt tgcagtgatt cattaagcac attaataagt aaatcaataa
     1141 aataagttaa atcagcactt ttctttactt cggcgaaaac atcatcacgc gcatcacggt
     1201 atctttctaa gataagttca aagttaagat aaggcgcaat aagcgcgagt tctctttctg
     1261 caaggaaggc ttttgccatt aaaatggcaa taatttcact atgagcttca aaaggtttaa
     1321 tataatcaag atagaaaata atcgctgcac tttgcacaat tgttggtaat gtacttgtgg
     1381 ccataaatgc gtaaaaatca gcgattgcat cactaatttg attgacggga acatgcggat
     1441 gaacatgatt aatgacggag acttgacgcc ccgttttaat ttcggtagtg cgataaatat
     1501 agttacttag cggcggaaca ttaaataggc gcccaacatc agctaaaagt gcttcagtaa
     1561 aatcaagatt agcgcttttt gctactagtt ctaacccgcg ataataatca tttaaaaaca
     1621 tctcccacgc atttaaaaca ctaatattgt gattaatgag ttgattaaca atcccatcat
     1681 gaaaattaat ttcataacta gcggcgattg tttttaaaat atcacgagtt aataaggttt
     1741 taagtgcctt ttgatgatta gcgtcaacat aaacttcttg aattcgtgct tgaatacgcg
     1801 agattcgccg gtcaagttga tttaagcgat cattaatt
//
LOCUS       DAOPCD010000186         1833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_50323_length_1833_cov_3.352081, whole genome shotgun sequence.
ACCESSION   DAOPCD010000186 DAOPCD010000000
VERSION     DAOPCD010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50323_length_1833_cov_3.352081"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLQ55_04475"
     CDS             complement(<1..319)
                     /locus_tag="PLQ55_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPK29219.1"
                     /translation="MKKRLAVIGGGVSGVTLARLVQGSFNVTIFERESSLLQKLLRTG
                     NGRANIYNRNIHSFAYNDEHFLSEHLPHIVKTLDDLFSAFNIVTYTDTEGRVYPYSRS
                     AKAL"
     gene            complement(316..>1833)
                     /locus_tag="PLQ55_04480"
     CDS             complement(316..>1833)
                     /locus_tag="PLQ55_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPK29220.1"
                     /translation="IRTIMKRKYVFLPLLLLGSVSLSSSPSPRVTHFAPHYAAPSTEP
                     SGPQTLRIYNWEEYISEEDIDDPDCIDVLAAFQEYYLENHGEEVTIEYNTFSTNEDMY
                     NIVKLNGAKYDLIAPSDYMIQRMIREDMLEKYDLDAEGNYKYVPNYNEYASPYLIDLF
                     KEDLGIYEYAPGYMWGTMGFLYNSEFNDTINDDVQSWMMLWDKKYSKKTSIKDSMREG
                     YLMGLFKYYEDELNDLAAQREAGLISDDYYNHELSDLLNDVDPDTIKEVIHVMNELKN
                     NIYGLEVDQGKIDMITGKIAANTAWSGDAVYAIEEDPNDVLRYAIPKEGSNIWFDGWI
                     MPKGANRVVAQRFVNFVSSPEMAIANMYYIGYTSFIAGDEVLEYVLDNDAVYEAAEAE
                     ETVELDFTYFFSDTIENPSDALITIDASYVGHTLTATYPTEEEIVRSVVMRDFGDSNK
                     LVIDMWANFKATDIQNWMIIFSVIIVLAVGSAVTLNQVQKHKAARRKRNLYKDKQ"
BASE COUNT          542 a          399 c          300 g          592 t
ORIGIN      
        1 ggagtgcttt agcacttctg ctatagggat atactcttcc ttctgtatcc gtgtaagtga
       61 caatattaaa agcactaaat aagtcatcaa gcgttttaac gatatgtggt aagtgttcac
      121 ttaaaaagtg ttcatcatta taagcaaagg aatgtatgtt gcgattataa atgttcgcgc
      181 gtccattacc cgtgcgtaaa agtttttgta agagactaga ttcacgctca aagatggtga
      241 cattaaagga accttgcaca agtcgcgcta aggtaactcc gcttactcca ccaccgatga
      301 ctgctaatct ttttttcatt gtttatcttt atataaattg cgcttacgcc gcgctgcctt
      361 atgtttttgg acttggttaa gggtgacggc actaccaact gctaaaacga tgataacact
      421 aaagataatc atccagtttt gaatgtccgt cgctttaaag tttgcccaca tatcaatgac
      481 gagcttatta ctatcaccaa agtcacgcat gacgacactg cggacgattt cttcttccgt
      541 cggataagtc gcggttaatg tatgaccaac gtaactcgca tcaatcgtaa ttaaggcgtc
      601 actaggattt tcgattgtat cactaaagaa atatgtaaag tcaagctcaa ctgtttcttc
      661 tgcttctgct gcctcataca ctgcatcgtt atcaagcaca tattcaagta cttcatcccc
      721 cgcaataaaa ctagtgtaac caatgtaata catattagcg attgccattt ccggactact
      781 cacaaagttc acaaagcgtt gtgcgacgac acgattagcg cccttgggca taatccagcc
      841 atcaaaccaa atattactac cttcttttgg aatagcgtaa cggagcacat catttgggtc
      901 ttcttcaatg gcatacactg cgtccccgct ccacgcggtg ttcgcagcga ttttcccagt
      961 aatcatatca atcttacctt ggtcaacttc taacccataa atattatttt taagttcatt
     1021 catcacgtga atcacttctt taatcgtatc agggtcaaca tcattaagta ggtcgcttaa
     1081 ctcgtgattg taataatcat cactaataag ccctgcttcc ctctgcgcgg ctaaatcatt
     1141 aagttcatct tcgtaatatt taaaaagtcc cattaaataa ccttcgcgca tactatcttt
     1201 gatcgaagtt ttcttactat attttttatc ccataacatc atccaacttt ggacatcgtc
     1261 attaatcgtg tcattaaatt cactattata gagaaaaccc attgttcccc acatataccc
     1321 tggggcgtat tcataaattc ctaaatcttc tttaaaaaga tcaattaaat atggacttgc
     1381 atattcatta taattaggaa catatttata attaccttct gcgtcaagat catacttttc
     1441 gagcatatct tcacgaatca tccgttgaat catatagtca cttggggcaa ttaagtcata
     1501 ctttgcgcca ttgagtttga cgatattata catatcttca ttcgttgaaa atgtattgta
     1561 ctcaattgtg acttcttcac catgattttc taaataatat tcttgaaagg ccgctaaaac
     1621 atcaatacaa tctggatcat caatatcttc ttcactaata tattcttccc agttataaat
     1681 gcgtaaagtt tgcggaccgc ttggctcagt gcttggagcg gcatagtgcg gcgcgaaatg
     1741 cgttactctt ggcgaaggtg aactacttaa acttacacta ccaagtaata atagtggtaa
     1801 gaaaacatat ttacgtttca ttatagtacg tat
//
LOCUS       DAOPCD010000187         1832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_50358_length_1832_cov_3.704558, whole genome shotgun sequence.
ACCESSION   DAOPCD010000187 DAOPCD010000000
VERSION     DAOPCD010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50358_length_1832_cov_3.704558"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PLQ55_04485"
     CDS             <1..763
                     /locus_tag="PLQ55_04485"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HPK29221.1"
                     /translation="ADKANTKRYVFGQLNKIDFTVFQFGVLLFGIELYKQLIFLNLYE
                     GLANYHWYGFPLQFCSIPIFLYPLTPFIKNEKIKEAIYSFISIFNLIGGLAVMILATG
                     VYTLQVSISIHTMIWHGVMVVVAFYLINAYKIGTKWRHYLGAVTVLFCLIVLAQLTNV
                     LFHYIGMKFPGPGDFDGFFISPWIDRRNMPILGDIRANMIAGGVPTLIIALVFPHIYF
                     VIFSLTGLLIYYLFHFIWKDVEKNKKEKALKTNTL"
     gene            768..1358
                     /gene="rdgB"
                     /locus_tag="PLQ55_04490"
     CDS             768..1358
                     /gene="rdgB"
                     /locus_tag="PLQ55_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPK29222.1"
                     /translation="MKKQIVIATNNANKLKEFKSLFANTPYEVLSLADINFTGVIKED
                     GESFSDNAVIKAMTIHKLTNLPVIADDSGLSVNALEGFPGVNSARFMEGSPYTEKNAA
                     IIKMLEGHSDKSAKFICAIALVGLDSFPQVFIGEVKGTIINEARGEEGFGYDPIFFHP
                     NSQKTFAEMTLEEKDEISHRGRATALLFEYLETHYF"
     gene            complement(1355..1759)
                     /locus_tag="PLQ55_04495"
     CDS             complement(1355..1759)
                     /locus_tag="PLQ55_04495"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HPK29223.1"
                     /translation="MEQKEIKNVVAALIFYDGKLFAASRSEKDERYGKYEFPGGKIEL
                     EENPSSAIVREIKEELMMDISVKDLFMHVSHEYPWFKLEMDVFICEVTNPQFTLLVHK
                     DGGFFTKEEILKLPFLGADLQVLAEISKRLDF"
BASE COUNT          575 a          306 c          319 g          632 t
ORIGIN      
        1 agccgacaag gcgaacacga aacgctatgt ttttggtcag ttaaataaga ttgattttac
       61 tgtttttcaa tttggcgttt tactatttgg cattgaatta tacaaacaac taattttctt
      121 aaatttatat gaagggcttg ctaattatca ttggtacgga ttccccttac aattttgttc
      181 aataccaatc tttttatatc cacttacacc atttattaaa aatgagaaga ttaaagaagc
      241 catttactcc tttatttcaa tcttcaatct tattggtggt cttgctgtga tgattcttgc
      301 cactggtgta tatactctac aagtttctat atcgattcat acgatgattt ggcacggagt
      361 aatggttgtt gttgctttct atttaataaa tgcttataaa attggcacta aatggcggca
      421 ttatcttggc gcggtcacag tattattttg tttgatcgtt ttagcgcaat taacgaatgt
      481 tttattccac tatattggta tgaagttccc tggtccaggg gattttgatg gtttctttat
      541 ttcaccatgg attgacagga gaaatatgcc gatacttggt gatattagag caaatatgat
      601 tgctggtggt gtccccacac taatcattgc actagtgttc ccgcatattt actttgtaat
      661 ctttagttta actgggctac ttatttacta tttattccat tttatttgga aagatgttga
      721 aaaaaataaa aaggaaaaag cattaaaaac taatacttta taaaattatg aaaaaacaaa
      781 ttgtcattgc cacgaataac gcaaataaac ttaaagaatt taagtcactt tttgctaata
      841 cgccttacga agttttaagt ctggcggaca ttaactttac tggcgttata aaggaagacg
      901 gcgaatcgtt tagtgataat gcggttataa aagctatgac tattcataaa ttgacgaatt
      961 tacctgttat cgctgatgat agtggactaa gtgttaacgc tttagaaggt tttcctggtg
     1021 ttaattctgc gcggtttatg gaaggctcac cttatactga gaaaaacgct gcaattatta
     1081 aaatgcttga aggccacagt gataaaagcg caaagtttat atgtgctatt gcccttgtcg
     1141 gccttgacag tttcccgcaa gtgtttattg gcgaagttaa aggcactata ataaacgaag
     1201 cgcgtggcga agaaggattt ggttatgacc cgatattctt tcatccaaac agtcaaaaaa
     1261 catttgccga aatgacgctt gaagaaaaag atgaaattag ccatcgcgga agggcgacgg
     1321 cattactatt tgaatattta gaaacgcatt atttttaaaa gtctaaacgc ttactaattt
     1381 cagcaagcac ttgaagatca gcccctaaga agggcaattt taatatttct tcttttgtaa
     1441 aaaaaccacc atctttatgc actaataagg taaattgcgg gttagtaact tcacaaataa
     1501 aaacatccat ttcaagttta aaccatggat actcatgact tacatgcata aataagtctt
     1561 tgacacttat atccatcatt aattcttctt taatttcgcg tacaatagca gaacttggat
     1621 tttcttctag ttcaattttt ccaccgggaa attcatattt accgtagcgt tcatcttttt
     1681 cacttcgtga agcagcgaaa agtttaccat catagaagat aagggccgcc acaacatttt
     1741 taatttcttt ttgttccata ctttattagt ataacaaagt aacatatttt tacgctgtaa
     1801 attgatatcg ggatatattt actgataaaa cg
//
LOCUS       DAOPCD010000188         1825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_50657_length_1825_cov_3.759322, whole genome shotgun sequence.
ACCESSION   DAOPCD010000188 DAOPCD010000000
VERSION     DAOPCD010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50657_length_1825_cov_3.759322"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..862)
                     /locus_tag="PLQ55_04500"
     CDS             complement(8..862)
                     /locus_tag="PLQ55_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013523906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPK29224.1"
                     /translation="MKKSNIKANRSIKRAKLFSDISTYVILVLGSVLMLAPLIWMLSA
                     SLKVNQFSVYGPLFHEWNFSNYIEVFKTIPVWKGLLNSVIVAVPSIIVGTFVSGLAAY
                     AFAKIKFKFCNIIFILMLAVMMIPFPVIMIPQYYIFSDLDWIGTLLPLIIPKLFGNIM
                     MIFFLRQFLTHIPDALVESAKIDGANHFQIFWIIILPLLKPALAAQTVLWFIGIWNDY
                     LAPNFFSAASPTLPVVIGGLVSDFPIQEPVHLNMAASIISMIPVLAIFAIFQKQIINS
                     FVLSGMKE"
     gene            complement(859..1767)
                     /locus_tag="PLQ55_04505"
     CDS             complement(859..1767)
                     /locus_tag="PLQ55_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPK29225.1"
                     /translation="MNTNIVEKPKKRKVKHVQENIIGYLFILPVFIGIAFFIVYPFFY
                     SLVSTFKNWNGLVPLSVAPTVGFEMYRRVLSDSLFWQAFGNTLITLIGIPIGMILSIA
                     IAMLLNRGLKFTGIFRVIFYIPVVSSITAIIILWRAMMNTQGPINDFLALFGVKAINF
                     LGEPGWARFSLIMMLVWKGLGTSILLYLAGLQGISDTYKEAAKIDGANDWQIFWHVTF
                     PLLGPTHFFIVVTGIIGGMQLYVEPELLLGGGPSRATTTLLIYLFERFGAHRISEASV
                     VAWVLGVFVFIITAIQFYTNYRREKA"
BASE COUNT          675 a          344 c          308 g          498 t
ORIGIN      
        1 ctccttctta ttctttcatt cccgagagaa cgaatgaatt aataatttgt ttttggaaaa
       61 tagcaaaaat tgcgagaact ggaatcattg aaataattga cgctgccata tttaaatgaa
      121 ctggttcttg gattgggaag tcacttacta atccaccaat aacaacgggt aaagttggtg
      181 aagccgctga gaagaaattg ggggctaagt aatcattcca aatgccaata aaccataaca
      241 ctgtttgcgc cgccaaagcg ggttttaaaa gcggtaaaat aattatccag aaaatttgaa
      301 aatgattagc gccatcaatt ttagcactct caaccaaagc atcgggaata tgtgtaagga
      361 attgccgtaa gaagaaaatc atcataatat taccgaacaa ctttggaata ataagtggta
      421 ataaagttcc aatccaatct aaatctgaga atatgtaata ctgcggaatc ataataacag
      481 gaaaaggaat catcataacc gcaagcatta aaataaagat tatattacag aatttaaatt
      541 taatttttgc gaaagcataa gccgctaacc cagagacaaa tgtacctact ataattgacg
      601 gaacggcgac tataacacta tttaaaagac ccttccacac tggaattgtt ttaaacactt
      661 cgatatagtt agagaagttc cattcatgaa ataaaggacc ataaacagaa aattggttaa
      721 cttttaatga cgctgatagc atccaaataa gtggcgctaa cattaacact gatccaagta
      781 ccaaaattac ataagtgcta atatcggaaa agagtttggc acgcttaatt gaacgatttg
      841 ctttgatatt cgactttttc atgctttttc cctccgataa ttcgtataaa actgaatagc
      901 agtaataata aagacaaaca cacctagcac ccacgcgaca actgaagctt cgctaatacg
      961 gtgggcacca aaacgctcaa ataagtaaat taagagagtc gtggttgctc tacttggccc
     1021 gccacctaaa agtaattctg gctcaacata aagctgcatt ccccctataa taccggtcac
     1081 tacaataaag aaatgcgtcg ggcctaataa agggaaagta acatgccaaa atatttgcca
     1141 gtcattagca ccatcaattt tagcggcttc tttataagta tcagaaatgc cttgtaaacc
     1201 cgctaaataa agtaagattg atgttcctag acccttccat acaagcatca taataagcga
     1261 gaaacgggcc catcctggtt caccaaggaa gtttattgct tttacaccaa agagagctaa
     1321 aaaatcatta attggtcctt gggtattcat cattgctcgc cataatataa taattgcagt
     1381 aattgaggaa acaactggaa tatagaaaat tacacggaaa atgccggtaa atttaagtcc
     1441 gcggtttaac aacattgcaa ttgcaattga aagaatcatc ccaattggaa tcccaataag
     1501 ggtaataagg gtattaccaa aggcttgcca aaataatgaa tcacttaaca ctcgccgata
     1561 catctcaaat ccaactgtcg gcgcaacact aaggggaaca agtccattcc agtttttaaa
     1621 agttgaaacc aatgaataga aaaaaggata tacgataaag aaagcaatcc caatgaaaac
     1681 tggcaaaata aataaatatc ctattatgtt ttcttgaaca tgtttaactt ttcttttttt
     1741 tggtttttct acaatatttg tgttcataat atattactca tctaatctat gcccgaacat
     1801 cgaaaaaatc gatgttcgag caatg
//
LOCUS       DAOPCD010000189         1819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_50887_length_1819_cov_3.291950, whole genome shotgun sequence.
ACCESSION   DAOPCD010000189 DAOPCD010000000
VERSION     DAOPCD010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50887_length_1819_cov_3.291950"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PLQ55_04510"
     CDS             <1..665
                     /locus_tag="PLQ55_04510"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HPK29226.1"
                     /translation="VILIHYSAVYVEFFQNQGVAYASASYILPVYPCHIMMWLLVIVA
                     FTRHKKSRFFEGLAEFVFVVGTLAALIGALFNMNFLNSSNPTFTNYFLFKGVFGHMVM
                     FFGTTYLFVFEYVKPRVEKTMISVIVGLLFFVADGIIINTLYAKFDIPPVNAMFLLEP
                     PFEDYPFINFFTIGSIGLILCFIGLLIYEHITLPKAEQWITKISTKSNKQDEDLTTDK
                     TN"
     gene            719..1519
                     /locus_tag="PLQ55_04515"
     CDS             719..1519
                     /locus_tag="PLQ55_04515"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HPK29227.1"
                     /translation="MFNLMHELFKDKDTGVFTFSAFSLCHIIYLLLIIGGIILVIFLY
                     KNKTQEAKTKLITTTVTIAFALYIADFFLMPFATGTIDIGKLPFHLCTLMSVMCFVVR
                     HNKKMEVFKTAFTLLGLIGGLMYITYPAGVAQADGYSYRIVQTVIYHGLMIAQGVFAL
                     VFDDLDLSWKNFKYDVIAVLAVAVWAYIGNTLYSGSNFWLSYEHDFNWFFVKHDGLYL
                     IDDEKDAFIAPLMSVSSVLAISALLRLIAIKLKSLFVKEQVLEAEKEA"
     gene            1635..>1819
                     /locus_tag="PLQ55_04520"
     CDS             1635..>1819
                     /locus_tag="PLQ55_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK29228.1"
                     /translation="MNNKTKSRRLRFRNILLLWLVGLAGQLCWNLENQWFNTFVYAEV
                     GPFPQIVNAMVAVSAIAT"
BASE COUNT          536 a          284 c          319 g          680 t
ORIGIN      
        1 tagttattct tattcactat agtgcagttt atgttgaatt ctttcaaaat caaggtgtag
       61 catatgcatc cgcttcttat attttaccgg tatatccatg tcacattatg atgtggttgc
      121 tcgtaatcgt tgcttttacg cgtcataaaa agtcccgctt ctttgaagga ctagcagaat
      181 ttgtattcgt tgttggcact cttgcggctt taattggagc actctttaat atgaattttt
      241 tgaatagtag taatccaaca ttcaccaact attttctttt taaaggtgta tttggtcata
      301 tggtgatgtt ttttggtaca acatacttat ttgtttttga gtatgtaaaa ccacgcgttg
      361 aaaaaacaat gattagtgtt atagttggac ttctcttttt tgtcgctgat ggtattatta
      421 ttaatacact ttatgcaaaa tttgatatcc cacctgttaa tgcgatgttt cttttagaac
      481 caccgtttga agattatccg tttattaatt tcttcactat cggttctatt gggctaattt
      541 tgtgctttat tggactgcta atttacgagc acatcaccct gcccaaagct gaacaatgga
      601 taactaaaat atcgactaaa tcaaataaac aagatgagga tttaactact gataaaacta
      661 attaagtcat ttttagaaag gaataattat tcactactaa taattaaaaa gatattatat
      721 gtttaatcta atgcatgaat tatttaagga taaagatacg ggtgtcttta ctttctcggc
      781 ttttagttta tgccatatta tttatttatt attaattatt ggtggaatca ttttagtaat
      841 tttcttatac aaaaataaaa cacaagaagc aaagacaaaa cttattacta ccactgtgac
      901 aattgccttc gcactttata ttgctgactt tttcctcatg ccttttgcta ccggcacaat
      961 tgatatcggt aagttaccgt ttcacctatg taccttaatg agtgttatgt gttttgtagt
     1021 gcggcacaat aaaaaaatgg aagtatttaa aacagccttt acccttcttg ggctaatcgg
     1081 cggattaatg tacattactt atcccgcagg tgtggcacaa gctgatggct atagttaccg
     1141 tattgtgcaa acagtaattt accacggtct tatgatcgca caaggggttt ttgcgctagt
     1201 atttgatgat ttagatttga gttggaaaaa ctttaaatat gatgttattg ccgtcttagc
     1261 ggtagcagta tgggcctata ttggtaatac gctctatagc ggaagtaact tctggctttc
     1321 ttatgaacat gactttaatt ggttttttgt aaagcatgat gggttatatt taattgatga
     1381 cgaaaaagat gcatttattg ccccattaat gtcggtgtca tctgtccttg ctattagtgc
     1441 cttacttcgt ttaattgcta ttaagcttaa aagcctcttt gttaaagagc aagttctcga
     1501 agcagaaaaa gaagcctaaa attcacttat atttcgtatt tttccgttta ttagagagct
     1561 tttttattta tttattaaag tagtttaaca tctagcaaaa ataagataaa atacatttaa
     1621 taggagaaaa aattatgaat aataaaacga aaagtcgtcg tttgcgtttt cgcaacattt
     1681 tactattgtg gcttgttggt cttgctggac aattatgttg gaacttagaa aaccaatggt
     1741 ttaatacatt tgtttacgct gaagttggtc cgtttccgca aattgtcaat gcaatggtcg
     1801 ctgtttcggc gattgctac
//
LOCUS       DAOPCD010000190         1818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_50928_length_1818_cov_3.401588, whole genome shotgun sequence.
ACCESSION   DAOPCD010000190 DAOPCD010000000
VERSION     DAOPCD010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50928_length_1818_cov_3.401588"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLQ55_04525"
     CDS             complement(<1..272)
                     /locus_tag="PLQ55_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016339053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPK29229.1"
                     /translation="MIGTNDLDNFEPVARIVVIGVGGAGNNAVNRMIDDQIDSVSFWV
                     ANTDKQALATSKAKNRIVLGEEFTHGLGAGGEPSVGRKAAEASRPE"
     gene            complement(275..1138)
                     /gene="pilM"
                     /locus_tag="PLQ55_04530"
     CDS             complement(275..1138)
                     /gene="pilM"
                     /locus_tag="PLQ55_04530"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HPK29230.1"
                     /translation="MALFRKNRVDPNKVIVAILPQVFKTDSDQVYRLLPLGEVSNTLT
                     VEAFLHVLPIWIYNSYIETVQKAGLKVRKVYPDKYALTELLNSPATATNTYFLVDFGA
                     KQTSITFVSNHNLYFSRIINEGSDDLTYLLAQKYGLELKEADRLKKDFGLDHRNVSVN
                     FPLATGIGERGEEIKVFLSDIYSTCQTFLNSHLQKVLSEIKIVGKSQNMPNLNKYPLL
                     FVGGGSTIYNYEEIIGSDRSFPDKYIVKLDTIGARDLTYAVNLGLIKAYSKYAQDIGD
                     EKASIGTLTRS"
     gene            complement(1096..1458)
                     /locus_tag="PLQ55_04535"
     CDS             complement(1096..1458)
                     /locus_tag="PLQ55_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29231.1"
                     /translation="MDIKISTLEISASAVKLVVGYELEGSPYVLYANKVALSQAISAG
                     EIVAPDQVINAIKVLAADAREALGETISEVVLALPTIDFEVFRGNKRQTRFRIKLIGL
                     ILKISWHFSVKTVLIQIK"
     gene            complement(1614..>1818)
                     /locus_tag="PLQ55_04540"
     CDS             complement(1614..>1818)
                     /locus_tag="PLQ55_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29232.1"
                     /translation="YKTNKKVGYYRFRAFLIGILLVAVGAAALTIPYPYVAKAINEAQ
                     ARRAQAEEESSEEAPLVTEDLTI"
BASE COUNT          562 a          373 c          299 g          584 t
ORIGIN      
        1 atttcgggac gactagcttc ggcggctttc cgcccaacac ttggttcacc gccagcacct
       61 aaaccgtgtg taaattcttc accaagcaca atacgatttt tcgctttact tgtggcaagt
      121 gcttgtttat cggtatttgc cacccagaaa gatacgctat cgatttgatc atcgatcatg
      181 cgatttacgg cattattacc agcgccaccg acaccaataa ctactattct agctacaggt
      241 tcaaaattgt ctaaatcatt cgttccaatc atagttaact ccttgttagt gtcccaatac
      301 ttgctttctc gtccccgata tcttgggcat acttactata agctttaatg agtcctaaat
      361 taacagcgta agttaagtct cttgccccaa tggtatcgag tttaacaata tatttatcag
      421 ggaatgaacg gtcactccca ataatttctt cataattata aatagtacta ccaccaccaa
      481 caaaaagcag tggatattta tttaagtttg gcatattttg actttttccc acaattttaa
      541 tttcacttaa taccttttgt aagtgactat ttaaaaatgt ttgacatgtc gaataaatat
      601 cacttaaaaa cactttaatt tcttcaccgc gttcaccgat ccctgtcgca agtgggaaat
      661 taacactaac attacgatga tcaagaccaa agtctttttt aagccgatcg gcttctttta
      721 gctcaagccc atacttctgc gctaaaaggt aagttaagtc atcacttcct tcattaataa
      781 tgcgtgagaa atataagtta tgattagaga caaaagtaat agaagtttgt tttgcgccaa
      841 agtcaactaa gaaatacgta ttagtggccg ttgcaggact atttaaaagt tcagttaagg
      901 catatttatc aggataaact tttcttactt taagtccagc tttttgcact gtttcaatat
      961 aggaattata aatccaaatc ggtaaaacat gtaagaaagc ttctaccgtt agcgtattac
     1021 ttacttcacc aagtgggagt aagcgataca cttgatcgct atctgtttta aaaacttgcg
     1081 gtaaaatcgc cacaattact ttatttggat caacacggtt tttacggaaa agtgccatga
     1141 tatttttaat atcaatccga tcaattttat tcgaaagcgt gtttgtcgtt tgtttcctct
     1201 aaacacttca aaatcaatcg ttggtaaggc tagcactact tcactaatcg tttcgcctaa
     1261 tgcttcacga gcgtccgctg ctaaaacttt aatcgcgtta atgacttggt caggcgcgac
     1321 tatttctccc gcacttattg cttgtgaaag cgccacttta tttgcataaa gcacataggg
     1381 actaccttca agttcataac cgacaacgag tttgacggca ctggcactaa tttctaatgt
     1441 actaatttta atatccataa ttctccaata atgtaattat tttacaataa ttaacgaata
     1501 ataacaattg caactaatta atatttgctt aattatcaca aatttaagaa aaaagcctta
     1561 gtgagagagg aaaaggggaa gagttcacta aggctttaaa gaaaggaaaa aaactaaatg
     1621 gttaaatctt cagtaactaa aggagcttct tcactgcttt cttcttcggc ctgagcacgg
     1681 cgtgcttgtg cctcgttaat cgctttagcg acatacggat aaggaatcgt taaagctgcc
     1741 gctccaaccg ctacaagtaa aatccctata agaaaggctc taaagcggta gtatccgact
     1801 tttttattcg ttttgtat
//
LOCUS       DAOPCD010000191         1816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_51003_length_1816_cov_4.982964, whole genome shotgun sequence.
ACCESSION   DAOPCD010000191 DAOPCD010000000
VERSION     DAOPCD010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51003_length_1816_cov_4.982964"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1755
                     /gene="argS"
                     /locus_tag="PLQ55_04545"
     CDS             136..1755
                     /gene="argS"
                     /locus_tag="PLQ55_04545"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPK29233.1"
                     /translation="MIDIRLQELIKDATVKLGLDIDQDTIVIETSKDSAHGDYATNIA
                     MQLARPLRKNPREVATLLINAMDQTPFEKIEIAGPGFINFFLKPETLSKYIATILKEG
                     SNFGNGSKKDTKVNIEFVSANPTGKLHLGHARGAAVGDTLARLFIKSGYEVIREFYVN
                     DAGVQIDNLGKSLYVRYLNLFGIEKELPKDAYHSTDLIAIAESLKAEIGDKYVKESKE
                     GLEYFKLEGTNKFLDIIKKDLQNFDVVFDVYSYETKVRAGNTIPKLLEKLAPFTYEED
                     GATFLKTSDFGDDKDRVIIKSNGDFTYFLPDIAYHLNKLSRGSSLLIDVLGADHHGYI
                     GRMKAALQMFNYPADTLEVQIIQLVRIIQDGKELKMSKRTGNAITLEELCEEVGVDAA
                     RYFFLAKSASTHFDFDLDLATESSSANPVYYAQYAHARLSAVLARGEKFSLDPSGEGL
                     KEAAELNLIKQLIAYPSAVSDATMTREPYKIANYIQKLASLVHSFYTEVRILDESNVQ
                     LTSARLGLSIAAQIVLKDALTLLGVSAPQTM"
BASE COUNT          624 a          279 c          353 g          560 t
ORIGIN      
        1 aatttaaaag tttcctaatc tttatttgta aagataagta tgtcatctaa caataatcac
       61 cttttttgct tgtgaaaagt ggatttactt tataatatac tttaaataaa gtatacttaa
      121 tcgttgaggt aagaaatgat tgatatccga ttacaagaat taattaaaga tgcaactgtg
      181 aaactgggac tagatataga ccaagacaca attgtgattg aaacgagtaa agatagtgct
      241 catggggatt acgctacaaa tattgcgatg caactagcgc gtccgttacg caagaatccg
      301 cgtgaagtag cgactttact cattaatgcg atggatcaaa cgccatttga aaaaattgaa
      361 attgctggtc ctggttttat taatttcttt ttaaaaccag aaacattatc aaaatatatt
      421 gcgactattt taaaagaagg cagtaatttt ggtaatggta gtaaaaaaga tacgaaagtt
      481 aacattgaat ttgttagcgc taatccgact ggtaaacttc atttaggaca tgcgcgcgga
      541 gccgcagttg gtgatacatt agcaaggctc tttattaaaa gtggctacga agttatccgt
      601 gaattttatg ttaatgacgc tggtgtacaa attgataatt taggtaaatc actttatgta
      661 cgatacttaa atttatttgg cattgaaaaa gagttaccaa aagacgctta tcatagtacg
      721 gacttaatcg ccatcgcgga aagtttaaaa gctgaaattg gtgataaata tgttaaagag
      781 agtaaagagg gtcttgaata ctttaagctt gaaggtacaa acaaattttt agatattatt
      841 aaaaaagatt tacaaaactt tgatgttgtc tttgatgttt atagctacga aacaaaagta
      901 agagcaggaa atacgattcc aaagttactg gaaaaattag cgccttttac ttatgaagaa
      961 gacggggcaa cattcttaaa gacaagtgac tttggtgatg ataaagatcg tgtcattatt
     1021 aaaagcaatg gtgactttac ttatttctta cccgacattg cttatcattt aaataagttg
     1081 agtcgtggct catcactttt aattgatgtc ttaggtgctg accatcacgg ttatattggt
     1141 cgcatgaaag cagcgttaca aatgtttaac tatcccgctg atacacttga agtgcaaatc
     1201 attcaactag tgcggattat tcaagacgga aaagagttaa agatgtctaa acggacaggt
     1261 aatgcgatta ctttagaaga attgtgcgaa gaagttgggg ttgatgcagc gcggtacttt
     1321 ttccttgcta aatcagcgtc cacccacttt gactttgacc tagatttagc aactgaaagt
     1381 tctagtgcaa atccagttta ttacgctcaa tatgcacacg ctagactcag cgccgtctta
     1441 gcgcgcggtg aaaagtttag tctagaccca agtggggaag gtttaaaaga agctgctgaa
     1501 cttaacctaa ttaagcaatt aattgcttac ccaagtgctg ttagtgatgc tacaatgact
     1561 agagagccat ataaaatcgc taactatatt caaaaactag cgagtttagt gcactctttc
     1621 tacactgaag ttcgtatttt agatgaaagt aatgtgcaac ttactagtgc acggttaggc
     1681 ttatcaatag ccgcacaaat tgtattaaaa gatgctttaa cacttttagg tgttagtgca
     1741 ccccaaacga tgtaaaatat aaaggagatt aactattctt aatcaagtta aatagtaaat
     1801 atatatcaaa aagatt
//
LOCUS       DAOPCD010000192         1801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_51675_length_1801_cov_3.403207, whole genome shotgun sequence.
ACCESSION   DAOPCD010000192 DAOPCD010000000
VERSION     DAOPCD010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51675_length_1801_cov_3.403207"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLQ55_04550"
     CDS             <1..453
                     /locus_tag="PLQ55_04550"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPK29234.1"
                     /translation="YLYSKGDKLPPGHFHIIVETLVKHSDGTYLVMQRDYSKKEYPGL
                     YEGSAGGSLISGETKKEGAIREVREETGLIINDVSPTYYFMSEKDGVISQGYIAIISG
                     TKDITYLKGETINHKWLTLKELKAFILTAEYNKAHASRLKTYLDTLED"
     gene            493..945
                     /locus_tag="PLQ55_04555"
     CDS             493..945
                     /locus_tag="PLQ55_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HPK29235.1"
                     /translation="MTNRKERTKGFFKTTILSFQHMFAMLGATVLVPMLVGLDVSITL
                     ITAGIGTIIFYFMTKKKVPVFLGSSFAYLPAMMAIMSGSGPIGSTLWQQKMGALVVAI
                     FFTRLVYLVFAGVITLIGVERVRKIFPPRVVGPIIIVLGMLLVPNFME"
     gene            1007..>1801
                     /locus_tag="PLQ55_04560"
     CDS             1007..>1801
                     /locus_tag="PLQ55_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HPK29236.1"
                     /translation="MTLITIIGISAFAKPKSFFKAAPILLGFFVGYIYAAIIGLVDFS
                     SLQNADIIVFQSIGDKLGFYRYLRFDWSAIIMMVPLAFVTLMEHIGDISANSTVCGKD
                     FFVDPGINRTLAGDGVAIMAASLLGGPPQTTYGENTAILVITENYEPKNIFYAAVLAI
                     FFGVFSIFGSVISTIPSEVIGGASIILFGMISASGLRMVIEHKADIGQTKDLLVVSIT
                     LAVGLGLGALSVASDILAQVGTDFDATLLKIMIGNIELSPLAVATFV"
BASE COUNT          529 a          315 c          385 g          572 t
ORIGIN      
        1 tacctatact ctaaaggtga taaactaccg cctggtcatt ttcatattat tgtcgaaaca
       61 cttgttaaac atagcgacgg cacttattta gtaatgcaac gtgattatag taaaaaagaa
      121 tacccaggtt tatatgaagg aagcgcaggt ggttctttaa taagtggaga aaccaaaaaa
      181 gaaggggcca tccgcgaagt aagagaagaa acgggactta taataaatga tgtaagtcca
      241 acatattatt ttatgagtga aaaagacggc gttataagtc aaggctatat tgcgataata
      301 agtggtacta aagatattac ttatttaaaa ggggaaacaa ttaatcataa atggttaacc
      361 ttaaaagagt taaaagcatt tattttaacc gctgaatata ataaagcgca cgcttcccgc
      421 cttaaaacat atttagatac attagaagat taataataaa cttagcaatt atttgctata
      481 gaagaggtat atatgacaaa tagaaaagaa cggaccaaag gattctttaa aacaacaatc
      541 ttaagtttcc aacatatgtt tgctatgctt ggcgcaacag tgttagtgcc gatgcttgtc
      601 ggtcttgatg tttcaatcac acttattacc gcgggtattg gtacaatcat cttttacttt
      661 atgacaaaaa agaaagtacc ggtattctta gggagtagct ttgcctatct tccagcgatg
      721 atggcaataa tgagtggcag tggcccgatt ggttcaacgt tgtggcaaca aaaaatgggt
      781 gctctagtgg tcgctatttt ctttaccagg ttagtttacc tcgtttttgc gggggtaatt
      841 acccttattg gtgtggaaag agtacgcaaa atatttcccc cacgcgttgt cggaccaatt
      901 attatcgtct taggaatgct tttagtgccg aattttatgg aataacatta ttgccccgta
      961 cgcgctttat tccggggaag cgtggaaatc atggacgact gcgcttatta ctttaataac
     1021 aattattggc attagtgctt tcgccaaacc aaagtccttt tttaaagctg ccccaatctt
     1081 gcttggtttt tttgtaggtt acatttacgc cgcgattatt ggcttagtgg acttttcttc
     1141 attacaaaac gctgatataa tcgtcttcca atcaattggg gataaattag ggttttatcg
     1201 ttatttacgt tttgattgga gtgcgattat tatgatggtg ccactcgcct ttgtaacttt
     1261 aatggaacat attggcgata taagtgcaaa tagtactgtc tgtggtaaag acttctttgt
     1321 cgatcctggc attaaccgta ccttagcagg cgacggcgtt gccattatgg ccgcaagctt
     1381 attaggtggt ccgccgcaaa cgacatacgg cgaaaacacg gctattttag taattaccga
     1441 aaactatgaa ccgaaaaata tattttatgc ggccgtgtta gctatcttct ttggggtctt
     1501 ctcgatattt ggttcagtta taagcactat tccgagtgaa gtaattggtg gcgcctcaat
     1561 tatcctattt gggatgatca gtgcttcggg cttacggatg gtaattgaac ataaagcgga
     1621 tattggacaa acgaaggatt tacttgttgt ttcgattact ttagcggttg gtcttggcct
     1681 tggcgctcta agtgtcgcaa gtgacatctt agcccaagtt ggtacagatt ttgacgccac
     1741 gctattaaag ataatgattg gaaatattga actttctccg ctagcggtcg ctacgtttgt
     1801 t
//
LOCUS       DAOPCD010000193         1778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_52675_length_1778_cov_5.374347, whole genome shotgun sequence.
ACCESSION   DAOPCD010000193 DAOPCD010000000
VERSION     DAOPCD010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52675_length_1778_cov_5.374347"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="PLQ55_04565"
     CDS             complement(<1..1526)
                     /locus_tag="PLQ55_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005344956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPK29237.1"
                     /translation="MARKQPFNINDLASVQIGLASPDMIREWSNGEILKPETVNYRSQ
                     KPELDGLFCERIFGPSKDYECHCGKYRRIRHRGTVCEKCGVEVISKYVRRERMGHIEL
                     ASPCTHIWYLKGTPARIATLLDIPAKQLEEIVYFISHIVTDPGTSSVLSYKQFIDETT
                     GRQVFAEAIKEIQENIEEGSFEHAKGSELLERLEVTQTFDFFATSRYISKHTGAKFGE
                     GAEVIKQLLKEVDLQKEFDEVNAALKTESKTKEKKLLRRLEVINAFLKSGNRPEWMVL
                     DVIPVIPPDLRPMLQLDGGRYATSDINDLYRRVISRNNRYKALVQMNAPKVILMNGQR
                     MLQEAVDSLIDNGRRGKPYTGANKRPLKSLSSSLKGKQGRFRQNLLGKRVDYSGRSVI
                     AVGPELKMYECGIPREMAIQLFRPFIAAELIKRDSRATNHTQADKIIDRHDEIVFDII
                     EEIIADHPVLLNRAPTLHRLGIQAFQPVLVSGRAIRLHPLVCAGFNADFDGDQMAVHV
                     P"
     gene            complement(1529..>1778)
                     /locus_tag="PLQ55_04570"
     CDS             complement(1529..>1778)
                     /locus_tag="PLQ55_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29238.1"
                     /translation="DLPTPGIPEAFKVLVRELQGLAINVELRMKDGEILDLDKLAKEQ
                     DADIRRSRRSTVVRDEQEVFDPEETGVIYRTEERGGDE"
BASE COUNT          469 a          393 c          353 g          563 t
ORIGIN      
        1 gggacgtgga cggccatttg gtcaccgtca aagtctgcgt taaagcccgc acacacaagt
       61 gggtgtaaac gaatcgcacg accactgaca agcacaggtt ggaacgcttg aatccctaaa
      121 cggtgcagcg tcggtgcacg gttaagtaat actgggtgat cagcgataat ttcttcaata
      181 atatcgaaga caatttcatc atgacgatca ataatcttat cggcttgtgt atgatttgtg
      241 gcgcgtgaat ctcttttaat gagttctgca gcaataaatg ggcggaataa ttgaatcgcc
      301 atttcgcgcg ggataccaca ttcatacatc ttaagttcgg gaccgacagc aataacacta
      361 cgcccactat agtcaacgcg tttaccgagt aagttttgac ggaaacgccc ttgtttacct
      421 tttaagcttg agcttaatga tttaagtgga cgtttattcg cgccagtgta tggtttgccg
      481 cgacggccgt tatcaatgag actatcaact gcttcttgga gcatccgttg cccgttcatg
      541 agaataactt ttggcgcatt catttgcact aaggctttat agcggttatt ccggctaatg
      601 acacggcgat ataagtcgtt aatgtcacta gtcgcgtatc tgccgccatc aagttgtaac
      661 attggccgta aatctggtgg aattacggga ataacatcaa gcaccatcca ttctgggcgg
      721 ttacctgatt taaggaaagc attaattact tctaagcgtc gtaataattt cttttctttt
      781 gttttacttt cggttttaag tgcagcgtta acttcatcaa attctttttg taagtcaact
      841 tcttttaaga gttgcttaat gacttccgca ccttcaccaa acttagcgcc agtatgttta
      901 ctaatataac gcgaagtcgc aaagaagtcg aaagtttgtg tcacttcaag tcgttctaaa
      961 agttcactac cttttgcgtg ttcaaagcta ccttcttcaa tattttcttg aatttcttta
     1021 atcgcttcag cgaacacttg acgccctgtt gtttcatcaa taaattgttt gtaagaaagg
     1081 acactacttg tgcctggatc agttacgata tggctaataa agtagacgat ttcttctagt
     1141 tgtttagcag ggatatctaa aagggttgca attcttgccg gtgtcccttt taagtaccaa
     1201 atatgtgtac acggactagc aagttcaata tgccccatcc gttcacgacg cacatattta
     1261 gaaataactt caacaccaca cttttcacaa actgtgccgc ggtggcgaat ccgtcgatat
     1321 ttaccacaat gacattcata atccttacta ggtccgaaga ttcgttcaca gaaaagacca
     1381 tcaagttcgg gtttttggct ccggtagtta accgtttctg gttttaaaat ttcaccattt
     1441 gaccattccc gaatcatgtc tggggaagca agtccaattt ggacacttgc taaatcatta
     1501 atattaaatg gttgttttct tgccataatt attcatcccc tcctctttct tctgttcgat
     1561 agataacacc tgtttcttct ggatcgaaca cttcttgttc gtctctaacc actgtgcttc
     1621 ttcgtgagcg acgaatgtcc gcgtcttgct ctttcgcgag tttatctaaa tcaagaattt
     1681 cgccatcttt cattcgtaat tcaacgttga tagctaaccc ttgaagttca cgcactaaga
     1741 ccttaaaggc ttctgggata ccaggagtgg gtaagtca
//
LOCUS       DAOPCD010000194         1774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_52849_length_1774_cov_2.995346, whole genome shotgun sequence.
ACCESSION   DAOPCD010000194 DAOPCD010000000
VERSION     DAOPCD010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52849_length_1774_cov_2.995346"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLQ55_04575"
     CDS             <1..295
                     /locus_tag="PLQ55_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPK29239.1"
                     /translation="IGGRASRVSLKSILRFSFLAYIIISVPTSVLGAVDLVHISEEIN
                     WTGYILAFIFTFGATFLTGLFVMKKLKVEHLIYFGIYCLVVGILAFSAFFIMR"
     gene            323..1048
                     /locus_tag="PLQ55_04580"
     CDS             323..1048
                     /locus_tag="PLQ55_04580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPK29240.1"
                     /translation="MSYRFFGRVRNNEVVLSDDDIFHLTKVLRLKKDETFEVVAGQSL
                     YLCTYESVNPFILNVLEKRKLPLEPLPRLTLGYALPKGDKLELVIQKAVEIGVSHIIL
                     IDSERSIGKIPATRLESRLARFNKIIKSAAMQANRNYLPTLTEPLAFKEVLDLEFDTK
                     LIAHETANLPLKAVLQQDLGDNLLILVGPEGGFSEAEVTLASTKGFNVITFGDNILRS
                     ETAAIYALSVLHHYQKGAHHENL"
     gene            1035..1742
                     /locus_tag="PLQ55_04585"
     CDS             1035..1742
                     /locus_tag="PLQ55_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29241.1"
                     /translation="MKIYELLQENRRGHLITPSREYLFFQDHEALLTQVPELIPFINS
                     KDDFDLICANIIQSALLNYEALTNYWNNEVKPDTELPVKALFTYNNVPIYCPLFSVSN
                     NILIGNNLGSKLNLTHDAIDLFEAYNFSLFESQLTSLVLVGEDIHTRAYYHYDFHAIY
                     IINDQGRLDLKICLFDKHIKRPDFRNVLERIKPALTEYYAGNRIAFINALVKEKLISH
                     KLYTKYINNLKRRKIIG"
BASE COUNT          572 a          256 c          336 g          610 t
ORIGIN      
        1 cattggtggt cgcgcaagtc gtgtgtcact aaaaagtata ttaagattta gtttccttgc
       61 ttatattatt attagtgtac cgacaagtgt gttaggcgca gttgacttag tacatattag
      121 tgaagaaatt aattggacgg gttacatctt agcgtttatc tttacttttg gtgcgacttt
      181 tctaacggga ctatttgtga tgaaaaagtt aaaggtggaa cacttaattt attttgggat
      241 ttattgttta gtcgttggta ttttagcgtt cagtgctttt ttcattatgc gttaatgcta
      301 aaatatatgt ggatttaaag ttatgagtta caggtttttt ggcagagtta gaaataatga
      361 agtagtctta agtgatgatg atatttttca tttaacgaaa gtattaaggc ttaaaaagga
      421 tgaaactttt gaagtagtgg caggacaaag tctttactta tgcacttatg aaagtgtaaa
      481 tccgtttata cttaatgttt tagaaaaaag aaagttaccg ttagagccct taccgcgctt
      541 aacactaggt tacgccttac caaaaggcga taaacttgaa ttagtgatcc aaaaagcagt
      601 ggaaattggt gttagtcaca tcattttaat tgatagtgag cgtagtatcg gtaaaatacc
      661 tgcaacgcgc ttagaaagtc gcttagcacg ctttaataag attattaaaa gtgcagcaat
      721 gcaagctaat cgtaactatc tgccaacgct tactgaaccc ttagcattta aagaagtttt
      781 agatttagag tttgacacta agcttattgc tcatgaaacc gctaatttac cgcttaaagc
      841 agtattgcaa caagatttag gcgataattt actcatttta gtcggtccag aaggcggttt
      901 tagtgaagcg gaagttactt tagcgagtac taaaggcttt aatgtcatta cttttggcga
      961 taatatactt agaagtgaaa ccgcagcgat atatgcttta agtgtgttac atcattatca
     1021 aaaaggagcg caccatgaaa atttatgagt tattacagga aaatcgccga gggcacctta
     1081 ttacaccgag tcgtgaatat ttatttttcc aagatcatga agcactgtta acgcaagttc
     1141 ctgaacttat tccttttatt aatagtaaag atgactttga tttaatctgt gcgaatatta
     1201 ttcaaagtgc actcttaaat tatgaagcac tcactaatta ctggaataat gaagttaagc
     1261 ctgatacaga attaccagta aaagcacttt ttacctataa taatgtaccc atatattgcc
     1321 cgctttttag tgttagcaat aacattttaa ttgggaataa tttaggaagt aaactaaatt
     1381 taacccatga cgcgatagat ttatttgaag cgtataattt tagtttattt gaatcgcaac
     1441 ttactagtct tgtgttagtt ggtgaagata ttcatacgcg cgcttattat cattatgact
     1501 ttcatgcgat ttatattatt aatgatcaag gacgacttga tttgaagatt tgtttatttg
     1561 ataaacatat taagcgccct gatttccgta atgtattaga acgaattaaa ccggcactta
     1621 ctgaatatta cgctggtaat cgcattgctt ttattaatgc tcttgtgaaa gaaaagttaa
     1681 ttagtcataa gttatacact aaatatatta ataacttaaa aagacggaag ataattggat
     1741 gaagaccttt actagtgtcg cacttggttg taaa
//
LOCUS       DAOPCD010000195         1769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_53017_length_1769_cov_3.603267, whole genome shotgun sequence.
ACCESSION   DAOPCD010000195 DAOPCD010000000
VERSION     DAOPCD010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53017_length_1769_cov_3.603267"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="PLQ55_04590"
     CDS             <1..862
                     /locus_tag="PLQ55_04590"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HPK29242.1"
                     /translation="LIACGSSNAEIETYPDWDRIVMETLYDHVDYISMHRYYWYEDSL
                     EDFFASYHDMDNFIHTIKSTADFVKAKKRSDKVMKISFDEWNVWYLNKWKPGGWKKAP
                     HILEDHYSLKDALVFSGLLNTLVNNVDRVEIAALAQLVNVIAPIFTKEGGGVFKQTIF
                     YPFYAVSNYGRGVALKAIKTGPKFNSKFGEVNYVSEAVTFNEEKREISVFLVNYAQEK
                     MEVELELRSFGRVEALTHKVMTHDDLEIKNTFDEENVKFVEGELPTVKNDVATLELGP
                     LSYHVYRFKY"
     gene            875..>1769
                     /locus_tag="PLQ55_04595"
     CDS             875..>1769
                     /locus_tag="PLQ55_04595"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPK29243.1"
                     /translation="MKRYLFLISVAILALTACSGGKTITSISNSDNIEEAIIYPTKPY
                     SDSDHIMQKDPYAFYNIAGNDTRYWGAANTHDPAIIKEGDTYYAFSTDANFGVTSQKG
                     IHIRKSNDLIKWSFVGTALDVTTLEDQIEYIGYNRDGEKVDFFWAPDIIKRGNEFWLF
                     YSISGFGQRNSMMGLAKSNNIEGPYTFDKEILRTSQSVGGTPNAIDSAIFVEEVDGEE
                     KMYMSYGSWSAGIYIIELDPVTGYPLIAQTLVEREVTVNTAEKGVTATATKLVPSSSD
                     DPAFGTKILSIRPREAPHTIKE"
BASE COUNT          580 a          279 c          363 g          547 t
ORIGIN      
        1 attaattgcc tgtggcagtt caaatgctga aattgaaaca tatcctgatt gggaccgtat
       61 tgtgatggaa acactttatg atcatgttga ttatatttcc atgcatcgtt attactggta
      121 cgaagatagt ttagaagatt tctttgcttc atatcatgat atggataatt tcattcatac
      181 aattaaaagc actgctgatt ttgtgaaagc taaaaaacgt tctgataaag taatgaagat
      241 ttcttttgat gaatggaatg tatggtattt aaataaatgg aaaccaggtg gttggaaaaa
      301 agcaccgcat attcttgaag accattattc acttaaagat gcattagtct tttcaggatt
      361 actaaatacg ctagtaaata atgttgaccg cgttgaaatt gctgctttag cgcaacttgt
      421 aaatgttatt gcccccattt tcacaaaaga aggtggaggg gtgtttaaac aaacaatttt
      481 ctatccgttt tatgctgtgt caaattatgg tcgtggtgtc gctttaaaag caattaaaac
      541 gggacctaaa tttaattcaa agttcggtga agtaaattat gtttcagaag cagtaacatt
      601 taacgaagag aaacgagaaa tttctgtatt ccttgtaaat tatgcgcaag agaagatgga
      661 agttgaactt gaattacgtt cgtttggtcg tgtagaagca cttactcata aagtaatgac
      721 gcatgacgac ttagaaatta aaaatacctt tgatgaagaa aatgtcaaat ttgttgaagg
      781 tgagttacca acagttaaaa atgatgttgc gaccttggaa ttaggcccgc tttcctatca
      841 tgtctatcgt tttaagtact agggaggtac tagtttgaag cgttatcttt tcttaattag
      901 tgtggcgatc cttgctttaa ccgcttgtag cgggggtaaa acaattacct ctatttctaa
      961 tagtgacaat attgaagaag caattattta tcctactaag ccatatagtg atagtgatca
     1021 cattatgcaa aaagatcctt acgccttcta taacattgcg ggtaacgaca ctcgctactg
     1081 gggagcggct aacacgcatg atccagcaat tattaaagaa ggtgatacat attacgcttt
     1141 ttcaacagac gctaattttg gtgtaacctc acaaaaaggg attcatatcc gcaaatcaaa
     1201 tgatttaatt aaatggagtt ttgtaggaac ggcgttagat gttacaacac ttgaagatca
     1261 aattgaatat attggttata acagagacgg ggaaaaggtt gatttctttt gggcgccaga
     1321 cattattaaa agaggtaatg aattttggtt attttattca atcagtggtt ttggacaacg
     1381 caattcaatg atgggattag caaagtctaa caatattgaa ggaccataca cttttgataa
     1441 agagatatta cgtacaagcc aaagtgtagg tggcacgcca aatgcgattg attccgctat
     1501 atttgtggaa gaagttgatg gcgaagaaaa gatgtatatg agttatggct catggtcagc
     1561 aggcatttac attatcgaac ttgatcctgt gacgggttat ccgcttattg cccaaacact
     1621 tgtcgaacgc gaagtaacag ttaacactgc tgaaaaaggt gtaacagcaa ccgctactaa
     1681 attagtgccc tcctccagcg atgatcctgc ttttggtacg aaaatattaa gtataagacc
     1741 tagagaagcg cctcacacca ttaaagaaa
//
LOCUS       DAOPCD010000196         1762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_53303_length_1762_cov_3.100176, whole genome shotgun sequence.
ACCESSION   DAOPCD010000196 DAOPCD010000000
VERSION     DAOPCD010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53303_length_1762_cov_3.100176"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLQ55_04600"
     CDS             <1..373
                     /locus_tag="PLQ55_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HPK29244.1"
                     /translation="ADMHNYGVVLNASTLIFTGESTIFENVKGAETHQTARIIVFDEN
                     SHAEANPILNIYHNDVVAASHAATVGQVNAEHLYYLKSRGLTEKEAKLLITKGYLMPI
                     LNYIEDKVLKENCITMLERVL"
     gene            373..1587
                     /locus_tag="PLQ55_04605"
     CDS             373..1587
                     /locus_tag="PLQ55_04605"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HPK29245.1"
                     /translation="MYDVYKIRKDFPVFDNNKTIYLDNAATTYKPQVVIDAVTNYLSK
                     ETANSGRGDYQNAYLVDKRVDETRALIHKFINSKHVQEVVFTQGTTASLNMVANCYGE
                     KFLKEGDEILLSIAEHASNTLPWMQVAKKTGAKIVYMPLSEDGKVTPEIVEKYMTEHT
                     KVVSVLHVSNVLGHVHGIKQIAKIVHDKGAIIVLDGAQSVPHMKVDVQDLDVDFLAFS
                     GHKMLAPTGIGVLYGKKELLEKMDPYQTGGGMNFSYDKEGNYELFKVPLKFEAGTLNI
                     EAIYGLHAAIEYINKIGIENISKHEHKLRTYAVSKLKEMDHIILYNENAETGIITFNV
                     KNVFAQDVATYFNSKNIAVRSGEHCAKLLKEHLREVATVRASLYLYTTKEEIDAFVEA
                     ARTAENYLDAYF"
     gene            1574..>1762
                     /locus_tag="PLQ55_04610"
     CDS             1574..>1762
                     /locus_tag="PLQ55_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPK29246.1"
                     /translation="MPIFNNELMRATILTHYENPYHKETPQNADEFMQIHLTSSGCID
                     NLTLYLKVKDNKVITGYFD"
BASE COUNT          623 a          263 c          342 g          534 t
ORIGIN      
        1 tgctgatatg cataactacg gtgtagtact taatgcatca actcttatat ttacaggtga
       61 atcaacaatt tttgaaaatg tgaagggcgc ggaaacccat caaaccgcac gcattattgt
      121 ttttgacgaa aatagccatg cagaggctaa tccaattttg aatatttatc ataatgatgt
      181 cgtcgctgca agtcacgccg ctactgtcgg acaagtaaat gctgaacatc tttattactt
      241 aaagtcccgc ggacttacag aaaaagaagc taaactttta attacaaaag gttacctgat
      301 gccgatatta aattatattg aagataaagt tttaaaagag aactgcatta ctatgcttga
      361 gagggtatta taatgtacga cgtttataaa attagaaaag attttccggt ttttgataat
      421 aacaaaacaa tatatttaga taacgctgct acaacatata aaccacaagt tgtaatcgat
      481 gcagtgacta attatttatc aaaagaaacc gctaatagtg ggcgcggcga ttatcaaaat
      541 gcttatttag tcgataaaag agttgatgaa acgcgcgctt taattcataa atttattaat
      601 agtaaacatg tgcaagaagt tgtttttacc caagggacaa cggcctcatt aaatatggtt
      661 gctaactgtt atggtgagaa gttcttaaaa gagggtgacg aaatattatt atcaattgct
      721 gaacacgcat ctaacacctt accctggatg caagtggcca aaaaaactgg tgctaaaatc
      781 gtctatatgc cacttagtga agacgggaaa gtcactccag aaattgtcga aaaatatatg
      841 acagaacata caaaagttgt cagtgtactt catgttagca atgttttagg tcatgttcat
      901 ggtattaaac aaattgcgaa aatagtgcat gataagggcg ctattattgt tttagatggt
      961 gcgcaaagcg tcccgcatat gaaagttgat gttcaagact tagatgttga ctttttagcg
     1021 tttagcggac ataaaatgtt agcgccaaca ggtatcggtg tactttatgg taaaaaagaa
     1081 ttattagaaa agatggatcc ttaccaaacg ggcgggggca tgaattttag ttatgataaa
     1141 gaagggaatt atgaattatt taaagtccca cttaagtttg aagcaggcac attaaacatc
     1201 gaggcgattt atggcttaca tgccgcgatt gaatatatta ataagattgg tattgaaaat
     1261 attagtaaac atgaacataa gttaagaaca tatgctgttt ctaaattaaa agaaatggat
     1321 catattattt tatataacga aaatgctgaa actggtatta ttactttcaa tgttaaaaat
     1381 gtgtttgcac aagatgttgc tacatatttt aatagtaaga atattgccgt tcgtagtgga
     1441 gaacattgcg ctaaattgct gaaagaacat ttacgcgaag tggcaactgt acgtgcaagt
     1501 ttatatttgt atacgacaaa agaagaaatt gatgcctttg ttgaggcagc tagaacagcg
     1561 gagaattatt tagatgccta tttttaataa cgagcttatg cgggcgacaa ttttaacgca
     1621 ttatgaaaac ccttatcaca aggaaacccc gcaaaatgcg gatgaattta tgcaaattca
     1681 ccttacttcc agtggttgta tagataattt aacactttat ttaaaagtta aagacaataa
     1741 agttatcacc ggatattttg at
//
LOCUS       DAOPCD010000197         1745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_54098_length_1745_cov_2.512426, whole genome shotgun sequence.
ACCESSION   DAOPCD010000197 DAOPCD010000000
VERSION     DAOPCD010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54098_length_1745_cov_2.512426"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1745)
                     /locus_tag="PLQ55_04615"
     CDS             complement(<1..>1745)
                     /locus_tag="PLQ55_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPK29247.1"
                     /translation="AHDANDFKIGERHNLSRPICLNKDATLNERGLELAGLDRFDARE
                     KLVAKMKELDLLIKVEKIVHNVGHSERSDAIIEPYLSKQWFLRMDHFAKKSIEGQKGD
                     DPIRFFPKRFNKTYLNWMEIAEDWCVSRQIWWGHRIPAYYHKETGEIVVSVTPPKDLE
                     NYRQDEDVLDTWFSSALWPFSTLDWPNTDSELFKRYYPTDVMVTGYDIIFFWVSRMIF
                     MGLELTNEKPFKDVVIHGLIRDEQGRKMSKSLGNGVDPIEVIAKYGVDSLRYFLAGAS
                     TPGQDARYSEARIQASVNYLNKIWNAARFLLLNLPEDFKPGKINHKKLHPVDAFILSR
                     YYETVRKVTSLMDKYELGMASNYLYAFVYDDYCSNYIEMVKVILNSDGDHTNTYNVMY
                     HTLHGILMMIYPYSPFISEEIYLALPNHLDSIMLASYPKASRLRFKREAEEVNTIINL
                     IKEIRNFKSTNNLAPNTKMNLYVADVSFDLTPYLDIIRRLGFINEFKYSDVPIEVTTV
                     SLPKATFGLELDVDRSVLISQLEKEIETLTKEVERSTKLLNNPGFKNKAPASKVAEEE
                     EKARHYAESLALAVD"
BASE COUNT          529 a          356 c          296 g          564 t
ORIGIN      
        1 ttatcaaccg cgagcgccag actttcagcg taatggcgag ctttttcttc ttcttccgca
       61 actttgcttg cgggagcttt atttttaaaa ccagggttat ttaaaagctt agtgctcctt
      121 tcaacttctt tcgttaaagt ttcgatttct ttttcaagtt gactaatcag cacactgcga
      181 tcaacatcta actctaagcc aaaagtagct tttggaagac taactgtcgt tacttcaata
      241 ggaacatcac tatacttaaa ttcattaata aaacctaaac ggcgaataat atcaagataa
      301 ggcgttaaat caaagctaac atcagcgaca tataaattca ttttagtatt gggcgctaag
      361 ttattagtgc tcttaaagtt acgaatttct ttaattaagt taataatcgt atttacttct
      421 tccgcttcgc gcttaaagcg taaacgacta gcttttggat aactcgctaa cattatagaa
      481 tcgagatgat ttgggagcgc taaataaatt tcttcactaa taaaaggtga ataaggataa
      541 atcatcatta agattccgtg taaagtgtga tacataacat tatatgtatt tgtatgatca
      601 ccatcactat ttaaaataac tttaaccatt tcaatataat ttgagcagta atcatcataa
      661 acaaaagcat agagataatt actagccata ccaagctcat acttatccat taaacttgtg
      721 actttacgca ctgtttcgta ataacgactt aagataaaag catcaacagg atgcagtttc
      781 ttatgattaa tcttacctgg tttaaagtct tctggtaagt ttaaaagtaa gaacctagct
      841 gcgttccaaa tcttatttaa gtaattgaca cttgcttgga tacgcgcttc actatagcgc
      901 gcatcttggc ctggggtact tgcgcctgct aagaaataac ggaggctatc aacaccatac
      961 ttcgcaatca cttcaattgg atcaaccccg ttgccgagtg attttgacat ttttcggccc
     1021 tgttcatcac gaattaaacc gtgaataacg acgtctttaa atggtttttc attagttaac
     1081 tctaacccca tgaaaatcat ccgtgatacc cagaagaaaa taatatcgta gcctgtcacc
     1141 attacatcgg tcgggtaata gcgtttaaag agttcgctat ccgtatttgg ccagtctagc
     1201 gttgaaaaag gccaaagtgc actgctaaac catgtatcaa ggacatcttc atcttgccga
     1261 taattttcta agtctttagg tggtgtaaca cttactacta tttcaccagt ttctttatga
     1321 taataagccg ggatacggtg accccaccaa atttggcgtg atacgcacca atcttcagca
     1381 atctccatcc aatttaaata agttttatta aagcgtttcg ggaaaaagcg aatggggtca
     1441 tcaccttttt gcccttcgat acttttttta gcaaagtgat ccatgcgtaa aaaccactgt
     1501 tttgataaat acggttcaat aatggcatca cttcgttcac tatgaccgac attatgcacg
     1561 attttctcca ctttaataag taaatcaagt tctttcatct tggcgacgag tttttcgcgt
     1621 gcatcgaagc gatctagacc cgctagttca agaccgcgtt cgttaagtgt tgcgtcttta
     1681 tttaaacaaa tcgggcgact taagttatgt ctttcaccga ttttaaagtc atttgcgtcg
     1741 tgcgc
//
LOCUS       DAOPCD010000198         1744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_54137_length_1744_cov_3.057430, whole genome shotgun sequence.
ACCESSION   DAOPCD010000198 DAOPCD010000000
VERSION     DAOPCD010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54137_length_1744_cov_3.057430"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1240)
                     /locus_tag="PLQ55_04620"
     CDS             complement(200..1240)
                     /locus_tag="PLQ55_04620"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPK29248.1"
                     /translation="MKIRRLLLALIGVTLLASCEPPTSQSIEVPESEIPSVSDISDTP
                     SIVSETSTTEEVTSVEESSEEEYSEWVEPEPVLKPAENWNFRPLPAYTGNFWNTIDFT
                     LRGEELRQALADYMWSKFYVPGVLDNRVTYDDALEAIKVMDADPNKTGNVLSLYDLRS
                     HPHAGYPGKWNREHVFPQSKLADGDKSLRASGNTKNISSDIANLFACDAALNEHKSNH
                     SYIEWNYAEDPERWFPYLSRNTAGQLVDAIVHYGYSPTPIVRGEIARAQLYMVLMYPD
                     RCSISENFQIADMIRWDQEWAPTIERDGQRQKGIEQYQKIRNPFIDNRNLSCYIWGNT
                     NRATRKLCADIY"
     gene            complement(1307..>1744)
                     /locus_tag="PLQ55_04625"
     CDS             complement(1307..>1744)
                     /locus_tag="PLQ55_04625"
                     /inference="COORDINATES: protein motif:HMM:NF021915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPK29249.1"
                     /translation="VNFKNTVIIMTSNLGSEFFLKNQTSKIDDLVKATFKPEFLNRID
                     EIVYFLPLSKDTQRQITRKMLDELMAHVAKNNFKLIYDEKIVDFVLDNAYAPEFGARP
                     LKRFIQKSLETFIAEQVVQGNIDSSITYRLTVTNNEVSISPFA"
BASE COUNT          514 a          349 c          319 g          562 t
ORIGIN      
        1 cccctgccag aatcgaacta gcgactgatc cttaccatgg atctgttatg ccatttaact
       61 aagggggcgt cgaattgaat tatagtacat aacgctaaat attacaataa atgtaataca
      121 tttaatgaat gaatagcctc tttttgcata aagaaaacgg cgagaatgac tcaccgttta
      181 attagtgcat taattatttc taataaatat ccgcacaaag tttgcgggta gcgcgattag
      241 tgttacccca aatatagcac gataaattac gattatcgat aaatgggtta cgaatctttt
      301 gatattgttc aatccctttt tgccgttgac cgtcgcgttc aatagtcgga gcccattctt
      361 ggtcccagcg aatcatatcg gcaatttgaa agttttcact aatgctacaa cgatctgggt
      421 acattagtac catatataat tgcgctctag caatttcacc acgcacaatt ggcgttggtg
      481 agtagccgta gtgcacaatc gcgtccacga gctgtccagc ggtattgcgt gataagtatg
      541 ggaaccaacg ctctggatct tcggcgtaat tccattcaat ataagagtga ttgctcttat
      601 gttcatttaa agcagcatca caggcgaata aattagcgat gtcacttgaa atatttttag
      661 tattaccact agcgcgtaaa cttttatcac catctgctag tttggattgc gggaaaacgt
      721 gctcacgatt ccatttaccg gggtacccag cgtgcggatg actacgtaag tcatacagtg
      781 ataatacatt acctgtttta tttggatcag cgtccattac ttttattgcc tctaaagcat
      841 catcgtatgt aactctatta tctaaaacac ctggcacata aaacttcgac cacatgtaat
      901 cggcaagtgc ttgacgaagc tcttcaccgc ggagtgtaaa gtcaatcgtg ttccaaaaat
      961 taccagtgta agcaggtagt ggtctaaaat tccaattttc agctggtttt aatactggtt
     1021 caggttcaac ccattcagaa tactcctctt cacttgattc ttcaacactc gtaacttctt
     1081 cagttgtaga tgtttcactg acgatacttg gtgtatcact aatgtcacta acgcttggaa
     1141 tttcactctc aggtacttca atactctgtg atgtaggtgg ttcacacgac gctaaaagcg
     1201 taactccgat cagcgctaat aataatcgac gtattttcat aattaaaaga cctcttttct
     1261 tatgtgattt aattataaca aagaggcctt tttttgtaaa ttaaagttag gcaaagggcg
     1321 aaatactaac ttcgttattt gttaccgtta agcgataagt aatactgcta tcgatattgc
     1381 cctgaacaac ttgttcagcg ataaatgttt ctaaactctt ttggataaag cgttttaatg
     1441 gtcgcgcccc aaattctgga gcgtaagcat tatcaagcac aaagtcaaca atcttttcgt
     1501 catatattaa cttgaagtta tttttggcaa catgagccat tagttcatca agcatcttgc
     1561 gcgtgatttg tcgttgtgtg tctttactaa gcggtaagaa atacacaatt tcatcaatcc
     1621 gatttaagaa ttcgggttta aaagtcgctt tcactaagtc atcaatcttt gatgtttgat
     1681 ttttaaggaa gaattcacta cctaagttac tcgtcataat aatgacagta tttttaaagt
     1741 ttac
//
LOCUS       DAOPCD010000199         1730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_54803_length_1730_cov_2.124179, whole genome shotgun sequence.
ACCESSION   DAOPCD010000199 DAOPCD010000000
VERSION     DAOPCD010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54803_length_1730_cov_2.124179"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..935
                     /locus_tag="PLQ55_04630"
     CDS             261..935
                     /locus_tag="PLQ55_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPK29250.1"
                     /translation="MSPLEIVLVSVLGALILIVAIYLFVVHTNAVNRLEIFEEVNKEA
                     KSGKIAFLGDSLTDFFPIQDFFPGVKIYNRGIAGDTTKNVLKRLDNVVALAPSKLFLQ
                     IGTNDLAKGSSPRKIVANIKKIITTFKKEVPNIEVICISLYPVSRTKKFLSTFIVGLR
                     TNKKIKKTNELLINFCAEEGLKYLDFYTLLADKKGRIDRRYTVEGLHISGKGYAVIAK
                     ALAPYL"
     gene            complement(937..>1730)
                     /locus_tag="PLQ55_04635"
     CDS             complement(937..>1730)
                     /locus_tag="PLQ55_04635"
                     /inference="COORDINATES: protein motif:HMM:NF015159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HPK29251.1"
                     /translation="LTLKLMKKIVIPLVMLLSLVGCKTEKLIRVATRGCIHEEILNAA
                     KTSLKEMGYRLNLETIDNALLANSSLSSGGIDANIIQRTDDLIAYNNRVSKRAELTSA
                     GRVYLSALGVYSGTKTTLEDYNPGDKIVISDRAEDYERIMSFIEEVFTVTVFTEQEKT
                     PNYRQTPLDFLTIEYMNPDLIPEAQKHPEEAFYIMTGSQAYNSGLSYEHILATETSND
                     YAFIVGIRRGEENNDRIRALMKVLYSDEIGDFIVEHFRGLIIPYR"
BASE COUNT          601 a          279 c          297 g          553 t
ORIGIN      
        1 aatataactg gcaaaatacc acaaaagtaa acaacaaaac accacaaaat atagtaatta
       61 ctttttaaaa caatcgtgta acgcataatt ttatgtgaaa accatttgtg ctcttaaggc
      121 ataaacaaag tacaaaaata gttttataat gtttgaagtg gaagagatta taatcaaaaa
      181 gtaaaaaagt ctttaattta taaaaagtct tatattatat ttgctaaaat aaagataatt
      241 gaaaagagga gctacttatt atgagtccac tagaaattgt gttagtaagt gtcttaggcg
      301 cacttatttt aattgttgcc atttatttat ttgtcgtaca cacaaatgca gttaatcgct
      361 tagaaatatt tgaagaagtt aacaaagaag ccaaaagcgg aaagattgct tttttaggtg
      421 atagtttaac agactttttt ccgattcaag atttctttcc gggtgtaaag atatataacc
      481 gcggtattgc tggtgatacg accaaaaatg tattaaaaag gcttgataat gtagtagcac
      541 tagcgccaag taagttattt ttacaaattg gcacgaatga cttagccaaa ggaagtagtc
      601 ctcgtaaaat agtcgctaat ataaaaaaga ttattacaac attcaaaaaa gaagtaccaa
      661 atattgaagt aatttgcatt tcactttatc ccgtatcacg cactaaaaag tttttatcca
      721 catttattgt tggattaaga acgaataaga aaattaaaaa gacaaatgaa ctcttaatta
      781 atttttgtgc tgaagaagga cttaaatatt tagatttcta cactctttta gcggataaaa
      841 aagggcggat tgaccgccgt tatacagttg aagggttgca tatttcaggt aagggctatg
      901 cggtaattgc aaaggcctta gccccttatt tataaattaa cgataaggaa taattaatcc
      961 gcggaaatgt tcaacgataa agtcaccgat ttcatcacta tataatactt tcattagtgc
     1021 gcgtattcta tcattatttt cttcgccgcg acgaataccg acaataaatg cataatcatt
     1081 actagtttca gtagcgagaa tatgttcata actaagtccg ctgttataag cttgtgaacc
     1141 agtcataatg taaaaagctt cttcgggatg cttttgggct tccggaatta aatctggatt
     1201 catatattca atggtcaaaa agtcaagtgg tgtttgtctg taatttggtg ttttttcttg
     1261 ttcggtaaat acagtaacag taaaaacttc ctcgataaaa ctcataatcc gctcataatc
     1321 ttcagcgcga tcactaatga caattttgtc gccaggatta taatcttcga gtgttgtttt
     1381 cgttcctgaa tatacaccaa gtgctgaaag atagacgcga ccagcactag taagctctgc
     1441 gcgttttgat acccggttat tataggcaat taaatcatct gttctttgaa taatatttgc
     1501 gtcaatcccg ccacttgata atgaagaatt agcgagcaaa gcattatcaa ttgtctcaag
     1561 atttaagcga taacccattt ccttaagtga agttttagcc gcgtttaaaa tctcttcatg
     1621 aatgcaacct ctcgttgcga cacgaattag tttctcagtc ttacaaccga ctaaggataa
     1681 gagcattact aacgggataa caattttttt cataagcttt aaggttagtg
//
LOCUS       DAOPCD010000200         1724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_55052_length_1724_cov_3.652487, whole genome shotgun sequence.
ACCESSION   DAOPCD010000200 DAOPCD010000000
VERSION     DAOPCD010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55052_length_1724_cov_3.652487"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1724)
                     /locus_tag="PLQ55_04640"
     CDS             complement(<1..>1724)
                     /locus_tag="PLQ55_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014094464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPK29252.1"
                     /translation="DSYRRFILMYFNVVKGQPRAEMEKMLEDMKVKAGVKLDSELKAE
                     HLRELVKQFKAHYKKVLGEEFPSDARVQLIEAVTAVFRSWDNERANIYRRMNHIPYEW
                     GTAVNVQSMVYGNKGDTSGTGVAFTRSPSTGDNHIFAEYLPNAQGEDVVAGIRTPLPI
                     SWLEENMPAVYKQFVETLDLLEKHYRDMQDIEFTVEDGKLYFLQTRSGKRTAAAALKI
                     VCDLIDEGLITEEEAVLRVDPKALDSLLHKGFDQAVLKTKKVIATGLPASPGAATGRL
                     SFDAADAAARKEKGETVLLVRSETSPEDIAGMVSAAGILTSRGGMTSHAAVVARGMGT
                     TCIVGAHDVIIDEVGHTMKVGDKVYTTKDVISIDGTTGNIYEGEIPLVEPQLTGYFGR
                     FMELADKYRRLEVRVNADTPLDATHGRDFGATGIGLCRTEHMFFDVDRVFFMRKMILS
                     ATKEEREAALAQILPYQENDFYEIFKVMKGLPVTVRLLDPPLHEFLPTEEAQIVALAK
                     ELKVDVGTLHERIELLSEFNPMMGHRGVRLAVSYPELAVMQTTAIISAALRAQKDTGK
                     KILPEIMI"
BASE COUNT          493 a          367 c          352 g          512 t
ORIGIN      
        1 aatcataatt tcagggagaa ttttcttacc agtgtctttt tgtgcacgta aggcagcact
       61 gataatagcg gttgtttgca taacagccaa ttctggataa gaaacagcta aacgaacacc
      121 gcggtgtccc atcatggggt tgaattcgga aagtaactca atacgttcat gaagagtacc
      181 aacatcaact ttgagttctt ttgccaaagc aacaatttgc gcttcttcgg ttggtaagaa
      241 ttcgtgtaac ggtgggtcaa gtaaacgaac agtgactggt aatcccttca tcaccttgaa
      301 gatttcatag aagtcatttt cttggtatgg aaggatttgc gctaaggctg cttcacgttc
      361 ttcttttgta gcggaaagaa tcattttacg cataaagaat acacgatcaa catcaaagaa
      421 catatgttcg gtacggcata agccgatacc agttgcacca aagtcgcgac catgggtggc
      481 gtcaagtggt gtatcagcgt taacacggac ttcaagacga cgatatttgt cggctaattc
      541 catgaaacga ccaaagtaac cagtaagttg tggttcgact agtgggattt caccttcata
      601 gatgttccct gtagtaccat caatactaat tacatcttta gtagtgtaaa ctttgtcacc
      661 aactttcatt gtgtggccga cttcatcaat gatgacatcg tgcgcaccaa cgatacatgt
      721 tgtacccatt ccgcgcgcaa caacagcagc gtgactagtc ataccaccgc gtgaggtgag
      781 aataccagca gcggaaacca taccagcaat gtcttctggt gaagtttcac tacgaactaa
      841 gaggacagtt tcgccttttt ctttacgggc agcagcatca gcggcatcaa agcttaaacg
      901 gccggtggca gcgcctggag aagctggtaa accagtagcg ataactttct ttgtttttaa
      961 gactgcttga tcaaagcctt tgtgaagtaa actgtcaagt gcttttggat caacgcgtaa
     1021 gacggcttct tcttcagtaa ttaagccttc atcaattaag tcacacacga ttttaagtgc
     1081 agcagcggcg gttcttttac cactgcgtgt ttgtaagaag tataatttac catcttcaac
     1141 agtaaattcg atatcttgca tatcgcgata gtgtttttct aataaatcta aagtttctac
     1201 aaattgtttg tagacggcag gcatgttttc ttctaaccaa ctaattggta gcggtgtacg
     1261 aataccagca acaacgtctt caccttgtgc atttggtaag tattccgcga aaatgtgatt
     1321 gtcaccagtg gatggtgaac gggtaaaggc aacaccggtt ccacttgtat cacccttgtt
     1381 accataaacc attgattgca cgtttacagc agtaccccat tcgtaaggaa tgtggttcat
     1441 tctacgatag atatttgctc tttcgttatc ccagctacgg aagactgcgg taacagcttc
     1501 aattaattgg acacgagcgt cacttgggaa ttcttcacca agaacttttt tataatgagc
     1561 tttaaattgt ttaactaatt ctcttaaatg ttcagccttt aattcactat caagcttaac
     1621 accagcttta actttcatgt cttcaagcat tttttccatt tcggcacgtg gttggccttt
     1681 aacaacgttg aaatacatta aaataaaacg acgatagctg tcaa
//
LOCUS       DAOPCD010000201         1718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_55331_length_1718_cov_4.349970, whole genome shotgun sequence.
ACCESSION   DAOPCD010000201 DAOPCD010000000
VERSION     DAOPCD010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55331_length_1718_cov_4.349970"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1522)
                     /locus_tag="PLQ55_04645"
     CDS             complement(77..1522)
                     /locus_tag="PLQ55_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPK29253.1"
                     /translation="MFKKKWMPLLLSIPLLLTGCKGKTSDSVTIFIYGQAHERSIYSK
                     IIEKFTEETGIKVNAELVNTDGYAETLTASLGTKNAPDVFYSNPASIGRYAMNGVILD
                     LKPYLEIEAEVEDGLDYKNFLDGVVDYYRYDVTTKKRGEGEAVWAIPKDVSSYPLGYN
                     RNIINLSVELGHWATHTKKGAWASREGGMPLPWENDPRTDKQIVYTFEEFTNACRATK
                     FIHNEIEYFGTALIDTWAMHSWIWAAGGNWLSDDGKTVTVNTPEFIAGFTEFIDLMDN
                     RGISQTREQMGVKPHYDRWMAGEVAFFNVGVWDVAAFESVPKELLDYGLMPTPRKDNA
                     SPEWYTYIGTLGYAVSSRTRVAQEAVKLALYMSMSTESYNRMTKYQTIQLPNNKLMVD
                     EYLSDETIKPENKGIFLDVIQKGHGKLHPTAMAYNSLWYDAFIAELSRVWKVTDDDPS
                     GEMSPAQYAAYVQPIMQAQLNIALADEAAGV"
     gene            complement(1536..>1718)
                     /locus_tag="PLQ55_04650"
     CDS             complement(1536..>1718)
                     /locus_tag="PLQ55_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29254.1"
                     /translation="EIYIPFSELGVTNVDSDYIKILHAVGSHEQNNMLPQEYLDLHPD
                     VNWDYPEDYPRISKPV"
BASE COUNT          506 a          371 c          296 g          545 t
ORIGIN      
        1 tgttcataat atattactca tctaatctat gcccgaacat cgagaggatc gatgtccgag
       61 caatgatata aataaattac acaccagccg cttcatcggc tagtgcaata ttaagttgag
      121 cctgcattat cggctgcaca taagcagcgt actgtgctgg cgacatttca ccacttgggt
      181 catcatcagt aactttccaa acgcgtgata attcagcgat aaaagcgtca taccataatg
      241 agttataagc catggctgtt ggatggagtt tcccgtgtcc tttttgaata acatcaagga
      301 aaattccttt gttttcaggt ttaattgttt catcactaag atactcatca accattagct
      361 tgttatttgg taattgaatt gtttgatatt tagtcatacg attatacgat tcggtgctca
      421 tactcatgta aagcgctagt ttaactgctt cttgcgcaac tcttgttcgt gaagaaacag
      481 cgtaacctaa tgtaccaata tatgtatacc actctggact agcattatct tttcgtggtg
      541 ttggcattaa accataatct aataattctt ttggtacact ttcaaatgcg gcaacatccc
      601 atacaccaac attaaagaag gctacttcac ccgccatcca gcgatcatag tgtggtttaa
      661 cccccatttg ttcgcgtgtt tgagaaatac cgcggttatc cattaaatca atgaattcag
      721 taaaaccagc gataaattct ggagtattga cagtaaccgt cttaccatca tctgataacc
      781 agttaccacc tgctgcccaa atccatgaat gcattgccca tgtatcaatt agcgcagtac
      841 caaaatattc aatctcgtta tgaataaact ttgtagcgcg acatgcattt gtaaattctt
      901 caaatgtata tacgatttgt ttgtcagtac gcggatcatt ttcccacggc aaaggcatcc
      961 cgccttcacg acttgcccat gctccttttt tcgtatgtgt tgcccagtga ccaagctcaa
     1021 ccgacagatt aataatatta cgattgtagc ctaaaggata agaggaaaca tcttttggga
     1081 ttgcccatac ggcttcccct tccccacgct ttttggttgt cacatcataa cgatagtaat
     1141 caacgactcc atctaagaaa tttttataat caagcccatc ctccacttct gcttctattt
     1201 ctaaataagg tttaagatcg agaataacgc cattcatcgc ataacgacca attgacgctg
     1261 ggtttgaata aaagacatca ggcgcgtttt ttgttcctaa agatgcagtt aatgtctcag
     1321 catacccatc cgtgttaacg agttcagcat tgactttaat tcccgtctct tcagtaaact
     1381 tttcaataat ctttgaataa atacttcgct catgagcttg gccataaata aagatagtaa
     1441 cactatcact tgttttacct ttacaaccag ttaaaagcag tggaatcgat agtaataatg
     1501 gcatccattt ctttttaaac atattattcc ctcctttaaa cgggttttga aatgcgtgga
     1561 taatcttcag ggtagtccca gtttacatct ggatgcaagt ctaagtattc ttgtggaagc
     1621 atattatttt gttcatgact gccaacagcg tgaagaattt ttatataatc ggagtcaaca
     1681 ttagtaacac cgagttcaga gaagggaatg taaatttc
//
LOCUS       DAOPCD010000202         1699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_56295_length_1699_cov_3.650243, whole genome shotgun sequence.
ACCESSION   DAOPCD010000202 DAOPCD010000000
VERSION     DAOPCD010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56295_length_1699_cov_3.650243"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1694
                     /locus_tag="PLQ55_04655"
     CDS             156..1694
                     /locus_tag="PLQ55_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HPK29255.1"
                     /translation="MKFLKNLKEALISSLPLLAVIILVAGIIFPVKTNTEIIQLVVGY
                     TGVVIGQAIFLTGLDYSVLPIGNLIGSSIVKLNKLVFVLMFAFLFSLLAAVAEPGITV
                     LAAQVGGIHPRINSTLFIWVTSFGMGIFTALAVYRLLKGVSMKKIFFFFYLAVIIIIF
                     FVPSQFQALGFDASGAATGAISVPFILALGVGVSHTVSRNKSAEDSFGIVGIAAIGPI
                     ITVYLYGLIVGNSDTLNVYTPGENSDLLAVMLGNLLPVALAVFPLMLIFLIFQAFFIK
                     LPRRKIGQIIIASLVVFVGLFIFIAATDFGLAFAGKYIGEQFMMSEKLWWLLLPLAFV
                     LGFAITLSEPSVVVFCEQVDELTSGSISKNTIKMALGISVGLATFFAVIKTILDINIL
                     WFIVPFYVITIGLIYFTPTLFVGLAFDGGTVSAGAITSAFLTPVTLGASQALGHTNIL
                     TSGLGILAFISVMPRIVVQILGIIYNYRLKKATPPVYEDISYDATTLSETETEIQEEA
                     TNDA"
BASE COUNT          485 a          257 c          349 g          608 t
ORIGIN      
        1 tttcaatatt aaaatcgtaa agtaatgata gtacgcatta tttaattatt aatgtaaaaa
       61 agttgtaagg agggcagtta taagttaatg ccatcttaaa atagtcgtaa tatgttatac
      121 ttacaaagta taaaaaagag gaagggtgta aatatatgaa gttcttaaaa aaccttaaag
      181 aagcacttat ctcaagttta cccctgcttg ccgttattat cttagtagcg ggaattattt
      241 tccctgtcaa aactaatact gagataattc aactagtggt aggatatacc ggtgttgtta
      301 ttggacaagc gatttttctt actggtctag attatagtgt cttaccaatt gggaacttga
      361 taggtagttc tatcgttaaa ctaaataaat tagtgttcgt cttgatgttt gcgtttttat
      421 ttagtttact cgctgctgtg gcggaaccag gcattactgt tttagctgca caagttggtg
      481 gtattcaccc acgtataaat agcacactct ttatttgggt aactagtttt ggtatgggaa
      541 tatttacggc cttagcggta tatcgtttgt taaaaggtgt aagtatgaaa aaaatcttct
      601 ttttctttta tttagcagtt atcattatta ttttctttgt tccaagtcaa ttccaagccc
      661 ttggctttga cgctagtggg gcggcaacag gtgcgattag tgttcccttt attttagcgt
      721 taggtgtagg tgtttcccat actgttagtc gcaataaaag tgcagaagat agttttggga
      781 ttgttggtat tgcggcaatt ggtccaatta ttactgttta cttatatgga ttaatcgttg
      841 gtaatagtga cacacttaat gtatatactc ccggtgaaaa tagtgattta ctagcggtaa
      901 tgcttgggaa tttattgcca gtcgcccttg cggtctttcc gcttatgtta atttttctta
      961 ttttccaagc attctttatt aagttgccgc gaagaaaaat tggtcaaata ataattgcat
     1021 cacttgttgt ttttgttggg ttatttattt ttattgcagc gacagacttt gggttagcgt
     1081 ttgctggtaa atatattggc gaacaattta tgatgagtga aaaactgtgg tggctacttt
     1141 taccactagc gtttgtactt ggctttgcaa ttactttaag cgaaccaagt gttgttgtat
     1201 tctgtgagca ggttgatgaa ttaactagcg gaagcatttc taaaaataca attaaaatgg
     1261 ctttaggtat ttccgttggc ttagcgacat tctttgcagt tattaaaaca attttagata
     1321 ttaatatctt atggtttatc gtaccttttt atgtaattac aattggttta atttacttta
     1381 caccaacatt attcgttggt ttagcctttg atggcggtac ggtaagtgcg ggcgcaatta
     1441 cgagtgcctt tttaaccccg gtgacactcg gggctagtca agctttaggg cacactaata
     1501 tattaacctc aggcttaggt attttagcct ttatcagtgt gatgccgcgt atcgttgtgc
     1561 aaatacttgg cataatttat aactatcgtt tgaagaaagc aacaccacca gtgtatgaag
     1621 atattagtta tgatgcgaca acgttaagcg aaacagaaac agaaatacaa gaggaggcca
     1681 caaatgacgc ataaagaag
//
LOCUS       DAOPCD010000203         1688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_56860_length_1688_cov_3.294550, whole genome shotgun sequence.
ACCESSION   DAOPCD010000203 DAOPCD010000000
VERSION     DAOPCD010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56860_length_1688_cov_3.294550"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PLQ55_04660"
     CDS             <1..611
                     /locus_tag="PLQ55_04660"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPK29256.1"
                     /translation="LIDNAGAWAWLIFSIIQIFSTVVLFIIPAQTTAFIVLALALFTP
                     LEAFLLVAINTLIASLLNFSVGKLFGKVIVQKIIGEETIIKYQEKLETKAKIYYPIMM
                     LFPFFPDDEITMLAGLTPMTYRYFIPVTFVTRSVGIATYVFLGSTFNYQNFTVLHWYQ
                     FIVIIGLLLYLVFFLADKLEKYLDSTPLSNFSFWRWSTRRKR"
     gene            760..1632
                     /locus_tag="PLQ55_04665"
     CDS             760..1632
                     /locus_tag="PLQ55_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPK29257.1"
                     /translation="MKKFSIVVNSSVDLTEELRKELDLVVAPLKFIMNGQEYINYLDY
                     RNLGVKEFYQKLRDGVVATTSQVNINEYVDIIEKEFKKGNDVLILSLSSALSSNYNSA
                     RLAVDMFKDHPEHKVYLVDTLTASLGEGLVAYAASMKRKEGATLEETYQFIEQFKYNV
                     STWFTVDDLMFLKRGGRLGGVSAIFGTLLAIKPILHVDLEGRLIPMEKTRGRKKALRI
                     IADKVKETYDPSLPNFCYVAHSDDEEAANLLKDMIEGLGIGLKVTLVNYVGPVIGAHV
                     GPGTIGAFFFAKRR"
BASE COUNT          515 a          262 c          315 g          596 t
ORIGIN      
        1 gacttattga taatgcggga gcatgggcgt ggttaatctt ttcaattatt caaatctttt
       61 caacggtcgt gctttttatt attcccgcac aaacgacggc ctttattgtc ttagccttag
      121 cgctttttac tccgcttgaa gcttttcttt tagtagcgat taatacactt atagcgtcgc
      181 tacttaattt ctctgttggt aagttatttg gcaaagtaat tgtacagaaa attattggtg
      241 aagaaacaat cattaaatat caagaaaaac ttgagacaaa agcaaaaatt tattatccaa
      301 ttatgatgct ttttccgttt tttcctgatg atgaaataac gatgcttgct ggtcttactc
      361 cgatgactta tcgctatttt atccccgtga cttttgtgac gcgttcagtg ggaattgcga
      421 cctatgtttt cttaggtagt acttttaatt atcaaaactt tacggtgctc cactggtatc
      481 aatttatcgt aattattggc ctccttttat atcttgtttt tttccttgcg gataaacttg
      541 aaaaatatct tgattccacg ccacttagta atttttcttt ttggcggtgg tcaacacgaa
      601 gaaaacgcta aatatataca cgtattaaaa taagctttta taatttgcgt aatgaacaat
      661 tatgaaaaat attcactttt gtggactttc aaattttttc tcgtatataa ttagatggct
      721 attaaatatt aatagcgcta caaaaggaga aatttattta tgaaaaaatt tagtattgtt
      781 gttaactcta gtgttgactt aactgaagag ttacggaaag agttagactt agtagttgct
      841 ccgcttaagt ttattatgaa tggtcaagag tatattaact atttagacta tcgtaattta
      901 ggagtcaaag aattttatca aaagttgcgt gacggtgttg tcgctacgac atcgcaagta
      961 aacattaatg aatatgttga tattattgaa aaagaattta agaaaggtaa tgatgtttta
     1021 attctttcac tttcaagtgc actttcaagc aactataata gtgcacgtct tgctgttgat
     1081 atgtttaagg atcatcctga acataaagtt tatcttgttg atactttaac agcatcactt
     1141 ggtgaaggct tagtcgctta cgctgcttca atgaaacgaa aagaaggagc gacacttgaa
     1201 gaaacttatc aatttattga acaatttaaa tacaatgtct caacgtggtt tacggttgat
     1261 gatttaatgt ttttaaagcg tggtggtcgt ttaggtggag tgtctgctat atttggtaca
     1321 cttttagcaa ttaaaccaat cttacacgtt gaccttgaag ggcgtctcat tccaatggaa
     1381 aaaacacgcg gtcgtaaaaa agctttacgc atcattgccg ataaagttaa agaaacttat
     1441 gacccaagtc tccctaattt ctgttatgtt gcgcatagtg atgacgaaga agcggcgaat
     1501 ttattaaaag acatgattga aggcttagga attggtttaa aggttacttt agtaaattat
     1561 gttggaccag ttattggggc gcatgtcggc ccaggcacga ttggtgcatt cttctttgca
     1621 aaacgccgtt aaatttgatt taaaagtaac gaaaggcttg caaattgcag gcctttttta
     1681 aataaata
//
LOCUS       DAOPCD010000204         1681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_57238_length_1681_cov_3.177122, whole genome shotgun sequence.
ACCESSION   DAOPCD010000204 DAOPCD010000000
VERSION     DAOPCD010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57238_length_1681_cov_3.177122"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PLQ55_04670"
     CDS             <1..793
                     /locus_tag="PLQ55_04670"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPK29258.1"
                     /translation="IKDLKAEVKLEHNVEEIVKVRDKYKVDGEEFDAVALTMGSSAGL
                     FKYPLDNGNAALLSALDLETTALVPTIKTIKVKENLKRIEHERVTARVTLMDDENVIA
                     SDVGEVLFKKDGLSGIVSFILSSYLEWAVVRYPKRNYYLTLDLMPEFSARYTAEIIKT
                     TDDLDNVFSPNISHYLKGRNEPLLDRMKALTFTPIPKNNPENTSAMHGGIKLSKLNTD
                     FEVRESPNLFALGEVINIDGICGGYNLAFAFYSAKVVSDALLRKL"
     gene            1059..>1681
                     /gene="nrdD"
                     /locus_tag="PLQ55_04675"
     CDS             1059..>1681
                     /gene="nrdD"
                     /locus_tag="PLQ55_04675"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPK29259.1"
                     /translation="MVTHIKKRDGRLQKFNAGKIANAINAAYGNTNDVQSLLYAAEIT
                     SNLNTNYDGTIDIEVVQDEVEKFLMKKDPNIAKKYILYRKDRERIRNAKSTVFNYKKL
                     VEDYLNIADWRVKENSTVNYSLGGLILSNSGAVTANYWLSEVYDEEVANAHRSAAIHI
                     HDLSMLSAYCAGWDIEKIIRDGVGGVEGKISSKPAKHLFTLANQLVN"
BASE COUNT          585 a          272 c          340 g          484 t
ORIGIN      
        1 cattaaagat ttaaaggcag aagttaagtt agaacataat gtcgaagaaa tcgttaaagt
       61 tcgtgataaa tataaagttg atggtgaaga atttgacgct gttgcgttaa caatgggcag
      121 tagcgcggga ctttttaagt atccgcttga taacggtaac gcggcgcttt taagtgcttt
      181 agatttagaa acaactgcgc tagtaccaac gattaaaaca attaaagtca aagaaaattt
      241 aaagcgcatt gaacacgaac gcgtcacggc aagagtgacg ctaatggatg acgaaaatgt
      301 tatcgctagt gacgtcggcg aggtgctatt taagaaagac gggttaagcg ggattgtaag
      361 ttttatttta agtagttacc ttgaatgggc tgtcgttaga tatccaaaac gaaactatta
      421 tttaacactt gatttaatgc ctgaatttag tgcgcgttac actgccgaaa ttattaaaac
      481 cactgatgac ttagataatg tctttagccc taatattagt cactatctaa aaggtagaaa
      541 tgaaccgtta ttagatcgga tgaaggcttt aacctttact ccaataccaa agaacaatcc
      601 agaaaacaca agtgcgatgc atggtggaat taagctttca aaattaaata cggactttga
      661 agtacgcgaa agccccaatc tctttgcttt aggtgaagtc ataaacattg acggaatatg
      721 cggtggctac aatttagcgt tcgcttttta ctccgcaaaa gtcgtaagtg acgcactctt
      781 acgaaaattg taaatatagc gcttccactt tatacaatgt ataaattact aattaaaacc
      841 tagcaaaaaa gggctatttt ggtcaaatta gtagacaatt agtagttatt tttctcgcga
      901 aatcacaaaa aatgaacaaa atttcgttac tgattaatta ctactttcga gaacaaatta
      961 ccgataagtg acaaaagttg caaagtcact ttttttgttt taatcttatg gacagaaatt
     1021 agaaataatt ttcttgagac acagagggag ggttttttat ggtcacacat attaagaaaa
     1081 gagacgggag attacaaaag tttaatgctg gtaaaattgc taatgctatc aatgcggcat
     1141 atgggaacac taatgacgtg cagtcattac tttatgcagc ggaaatcacc agtaatttaa
     1201 acacgaatta cgatgggaca atcgacattg aagtcgtcca agacgaagtc gaaaaattct
     1261 tgatgaagaa agatcccaac attgctaaaa aatatatttt atatagaaaa gatagagaac
     1321 ggattcgtaa cgctaaaagc accgttttta actataaaaa actcgttgaa gattatttaa
     1381 atattgccga ctggcgcgtt aaagaaaatt caaccgtcaa ttattcttta ggtggcctta
     1441 tcttaagcaa tagcggcgct gtaaccgcaa actattggtt aagtgaagtg tatgacgaag
     1501 aagtagcaaa tgcgcaccgc agcgccgcca ttcatattca tgacctaagt atgcttagtg
     1561 cttactgtgc gggttgggat attgaaaaga ttattcgtga tggtgttggt ggcgttgaag
     1621 gaaagatttc aagtaaacca gcgaaacact tattcacact cgctaatcaa cttgttaatt
     1681 t
//
LOCUS       DAOPCD010000205         1679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_57354_length_1679_cov_3.145936, whole genome shotgun sequence.
ACCESSION   DAOPCD010000205 DAOPCD010000000
VERSION     DAOPCD010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57354_length_1679_cov_3.145936"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..>1679)
                     /locus_tag="PLQ55_04680"
     CDS             complement(134..>1679)
                     /locus_tag="PLQ55_04680"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HPK29260.1"
                     /translation="LHKALAEVSKRIADILFKNDIKFEMKFRVKSIYSIYKKMKEKDN
                     DFERIYDLLALRIITPTELNCYEILGLIHATYRPVTGRFKDYIAMPKSNMYQSLHTTI
                     FTGDGNALEVQIRTPEMDRIADSGVAAHWAYKEGTNYNPEREQREIENKLHWLRDFVA
                     TGYETGNTAKEYMDTITKEIFETSVYVFTPKGNVIELPAGATPLDFAYKIHTKVGDTA
                     VGALVNDKLVAMNTPLKTGDICEIRTSKTATGPTESWLNIATTNSARSHIRKVLAKKN
                     QALTRDDNIEKGRGSLNDAFKERGFAEHEVEPHVNKPEVLKHFNVNTYEDLLLLVYSR
                     NITASAVLDYLKLDRRDHMGKLLSKGRRVVEDAKNPVIVPGAGTVAVTLGNCCTPVPG
                     DNIVGYITKGKGITVHRINCPNIARNLDRTIEVLWNPNSETKLYPLDISIKCNDRDNL
                     LVDIMNALSQAKVSISKIKATYHPSTQTSVIDATILIPNVDKLNSVQNIILQVDSVYK
                     IDRVIH"
BASE COUNT          477 a          337 c          280 g          585 t
ORIGIN      
        1 gaatatttgt taaaatccat atcaatgact ttttaattat aaaataatta actttaatag
       61 tcaatgtaag cggcgactat tattcactaa ttatctacta ttaaactact aataaaatac
      121 ttttagcgtt attttaatga attacgcggt caattttata aactgagtca acttgcaaga
      181 taatattttg cacactattt aatttatcaa cattcggaat taaaatcgtt gcatcaatca
      241 cacttgtttg ggtcgatggg tgataagtag ccttaatttt tgagatacta acctttgctt
      301 gacttaaagc attcataata tcgactaata agttatcacg gtcattacac ttgatactaa
      361 tatctaacgg atataattta gtttcgctat ttggattcca tagtacttca atcgtgcggt
      421 caaggttacg cgcgatattt ggacaattaa tgcggtgaac ggtaatccct ttccctttcg
      481 tgatataacc aacgatatta tcacctggca ctggtgtaca acaattacca agtgtgaccg
      541 caacagtgcc cgcccctggg acgattacgg gatttttagc gtcttctact actcttcgtc
      601 ctttacttaa aagtttgccc atatggtcac ggcgatcaag ttttaagtaa tctaatactg
      661 cactagcggt aatattccgc gaataaacaa gtaatagtaa gtcttcataa gtattaacat
      721 taaaatgttt taagacttct ggtttattca catgtggttc aacttcatgt tcggcaaagc
      781 cgcgttcttt aaaggcgtca tttagtgagc cacgcccttt ttcgatatta tcatcgcgcg
      841 ttaaagcttg atttttcttc gctagcactt tacgaatatg actgcgtgca ctattcgtgg
      901 tggcaatatt aagccatgat tctgttggtc ctgtagccgt tttactcgtt ctaatttcac
      961 aaatatcacc ggttttaagt ggcgtattca ttgctacaag cttatcatta acaagtgccc
     1021 caaccgctgt atctccgact ttagtgtgaa ttttataggc aaagtcaagt ggtgttgctc
     1081 ccgctgggag ttcaataaca ttaccttttg gcgtaaagac atacacacta gtttcaaaga
     1141 tttcttttgt aatcgtatcc atgtattctt tcgcggtatt accagtttca taacccgttg
     1201 ccacaaaatc acggagccaa tgtaatttat tttcaatctc acgttgttcg cgttctggat
     1261 tataatttgt accttcttta tatgcccagt gcgctgcaac ccctgagtca gcgatacggt
     1321 ccatctctgg cgttcttatt tgcacttcta aagcattacc gtcccctgta aagatagttg
     1381 tgtgtaaact ttgatacata ttgctttttg gcatcgcgat ataatcttta aagcgtcccg
     1441 taactgggcg ataagtcgcg tggattagtc ctaaaatttc ataacaatta agttccgttg
     1501 gagtaatgat tctaagcgct aaaaggtcat aaatccgttc aaagtcatta tctttctctt
     1561 tcatcttctt ataaatacta taaatcgact tcacacgaaa cttcatttca aatttaatgt
     1621 catttttaaa taagatatcc gcaattcgtt tactaacttc cgcaagcgcc ttatgtaag
//
LOCUS       DAOPCD010000206         1670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_57794_length_1670_cov_3.840248, whole genome shotgun sequence.
ACCESSION   DAOPCD010000206 DAOPCD010000000
VERSION     DAOPCD010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57794_length_1670_cov_3.840248"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /locus_tag="PLQ55_04685"
     CDS             complement(<1..1455)
                     /locus_tag="PLQ55_04685"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPK29261.1"
                     /translation="MDKKDYIIIKGARDNNLKNISVNIPKNKLVVFTGLSGSGKSTLA
                     LNTIYNEGQRRYVESLSAYARQFLGNLAKPDVDSIEGLSPAISIEQKSTSNNPRSTVG
                     TITEIYDYLRLLYARIGMPYCPQHDIPISSSSPEQILNKIFEYPEKTRIQILAPLVKA
                     EKGTHKDTLAKIAKEGFVRIRINGEIKALEEVGNLDKNKRHNIDLVINRVVLKAENKS
                     RIFEGINTALDYGHGYVIILFNNEETLFSTHNACHICGFSVPRLEPRLFSFNAPMGFC
                     PECRGLGLKREVDINILVPDKIVSLAKGAIEYYKNIVGTKNIEWQEFEKLCELYNIPM
                     HVPFKELSDKHKEIALFGSPNEHNFKIITGSGNTMYRRGKIEGVKNKIERLYEETTSE
                     FMRQYYEKFMRDVTCSTCNGKRLNEHALAVYINGKNIHDLTMLQIGELKQFLNETRPK
                     LSVTDRTISQLILDEIDHRLTFLVKVGLDYLTLAR"
BASE COUNT          486 a          321 c          271 g          592 t
ORIGIN      
        1 cctcgcaagt gttaagtaat ctaagccgac ctttactaag aaagttaaac ggtgatcaat
       61 ttcatcaagt ataagttgac taattgtcct atctgtcaca cttagttttg gtcgcgtttc
      121 atttaagaat tgctttaatt caccaatttg taacattgtt aagtcgtgaa tgttcttacc
      181 gttaatatac accgctagag cgtgttcgtt aagacgcttc ccgttacatg tcgaacaagt
      241 aacatcacgc ataaacttct cataatattg acgcatgaat tcacttgttg tttcttcata
      301 aagccgttca atcttatttt taacgccttc aatctttccg cgtcgataca ttgtattgcc
      361 gctaccagta atgatcttaa agttatgttc atttggacta ccaaaaagcg ctatttcttt
      421 atgtttatca cttaattctt taaatggaac atgcattggg atgttataga gctcacataa
      481 cttttcaaat tcttgccatt caatattttt agtaccgaca atatttttat aatattcaat
      541 cgccccttta gcaagactta caattttatc cggcactaaa atgttgatgt caacttcacg
      601 cttaagtcct aacccgcgac attctggaca aaaacccatt ggggcattaa agctgaatag
      661 tcgcggttct aaacgcggaa cactaaaacc acagatgtga cacgcgttat gcgtagaaaa
      721 aagtgtttct tcattattaa aaagaataat aacgtaccca tgtccataat ctagcgcggt
      781 attaattcct tcaaagatgc gtgatttatt ttcggctttg agcacaacac gattaatgac
      841 taaatcaatg ttatgacgtt tatttttatc aaggtttcct acttcttcaa gtgcttttat
      901 ttcaccatta attcgaatcc gcacaaaccc ttcttttgca atctttgcta gtgtatcttt
      961 atgtgtacct ttttcagctt tgacaagtgg cgctaaaatt tggattcttg ttttctctgg
     1021 atattcaaag attttattta agatttgttc gggtgaggaa ctactaattg gaatatcgtg
     1081 ctgcggacag taaggcattc caatccgtgc atataaaagc cgtaagtagt cataaatctc
     1141 cgtaattgtt ccaactgttg aacgcgggtt attactcgtt gacttttgtt caatgctaat
     1201 tgcgggacta agcccttcaa ttgaatcaac gtcaggctta gcgagattcc ctaaaaattg
     1261 ccgagcgtaa gcacttaagc tttcaacgta acgcctttgc ccttcgttat atattgtatt
     1321 taacgctagg gttgattttc cgctaccgga aagaccagtg aacaccacta gtttattttt
     1381 ggggatattc acactaatgt tttttaagtt gttatcccta gcgcctttaa taataatgta
     1441 gtctttttta tccatagctt tcattttacc ttattcataa ggtatttaga caaattatgc
     1501 acggtaagta aaaaaggtta agacacaatg ccgttaacca ctttaaaata aaaggcgaaa
     1561 atttatatta atgttcaaga acttttaagt ttactttgcc ttcttcttta attttaatcg
     1621 tacatgttcc atttggtact aaaaatgcgg ggcatttaat attatttttg
//
LOCUS       DAOPCD010000207         1667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_57946_length_1667_cov_3.305211, whole genome shotgun sequence.
ACCESSION   DAOPCD010000207 DAOPCD010000000
VERSION     DAOPCD010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57946_length_1667_cov_3.305211"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLQ55_04690"
     CDS             complement(<1..348)
                     /locus_tag="PLQ55_04690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPK29262.1"
                     /translation="MIEIRELKDNEISDFWQEHIKYLIEDGLIKEQSEIDYYSGLNYH
                     NKFEGYLKEATDKLHMVWFIKDGVKIGATQYKINKSGDGECYIYDFWVFPSYRGHNTG
                     HECFAALENYTKID"
     gene            complement(496..1185)
                     /gene="araD"
                     /locus_tag="PLQ55_04695"
     CDS             complement(496..1185)
                     /gene="araD"
                     /locus_tag="PLQ55_04695"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HPK29263.1"
                     /translation="MYKTLKETTYNANIAIKKSGLVLLTWGNVSVKDDVLKVIAIKPS
                     GVAYEKMKADDIVVVDFDGNIIEGTLRPSVDLPSHLALYKAHQEIGAIVHTHSTFATA
                     WAQKGRAIPMYGTTHADYFSDDIPCAPYLTPEEMKDYEKNTGEVIAALIKKGRANETP
                     AAIIKGHGAFAWGKNAKDAVLHAIVLEEVAKIAYLTEAIPGQNNPLPEHIKKTHYERK
                     HGPNARYGQKK"
     gene            complement(1189..1575)
                     /locus_tag="PLQ55_04700"
     CDS             complement(1189..1575)
                     /locus_tag="PLQ55_04700"
                     /inference="COORDINATES: protein motif:HMM:NF023190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29264.1"
                     /translation="MGGKKAPARLVFEGKPGKAKAISLIDLGHRFRLIALDVECVAPT
                     KSMPNLPVARLMWRPEPNLEKSAACWLVAGGAHHTTLVYDLSLEFLRDFANLLDIEFV
                     HIGKDADPYILEEMMKNNDIRYRLRG"
BASE COUNT          464 a          350 c          280 g          573 t
ORIGIN      
        1 gtcaatcttt gtataatttt caagggcggc aaagcattcg tgtcccgtat tatgaccgcg
       61 gtaagatggg aacacccaaa agtcataaat ataacattcc ccgtcaccac ttttatttat
      121 cttatactga gttgcgccga ttttaacccc atctttaata aaccatacca tatgaagttt
      181 atcggttgct tcttttaagt aaccttcaaa tttattatga taatttaatc ctgaataata
      241 gtcgatttca ctttgttctt taattaatcc gtcttcaatt aagtatttaa tatgttcctg
      301 ccaaaagtca ctaatctcgt tgtcttttaa ttctctaatt tcaatcatgc aaaaatacgc
      361 tattttctcc tattttcgat aaaaataatt atactcttac taaataaaag tgtatatttt
      421 ttactcaaaa aaaacaccct tcttaatact tttttacaaa gggtgccttt ttataattat
      481 ttaaaagttt agtgcttatt tcttttgacc ataacgagcg ttaggaccat gttttcgctc
      541 ataatgggtt ttcttaatat gctctggtag cggattattt tgacccggga ttgcttctgt
      601 aagataggca atcttcgcta cttcttctaa cacgatagcg tgcagcacag catctttagc
      661 atttttaccc cacgcaaaag caccatgtcc tttgatgatt gccgctggtg tttcattagc
      721 ccgtcctttt ttaatgagcg ccgcaattac ttcaccagta tttttttcgt agtctttcat
      781 ctcttctggt gttaaataag gagcgcacgg aatatcatcg ctaaaataat cagcgtgtgt
      841 cgtaccatac attggtattg ctctaccttt ttgtgcccac gctgtcgcaa atgttgagtg
      901 agtatggaca attgcaccaa tttcttgatg tgctttatat aaggctaggt gtgaaggcaa
      961 atcaacagac ggtcgcaaag ttccttcaat gatattcccg tcaaagtcga ctacaacaat
     1021 atcatctgct ttcatctttt catacgcaac accagacggt ttaattgcta ttactttaag
     1081 tacatcatct ttaacagaca cattgcccca tgttaaaagt actaaacccg atttcttaat
     1141 ggcaatgtta gcgttatatg tcgtttcttt taaagttttg tacatatttt accctcgtaa
     1201 acgatagcgg atatcgttat ttttcatcat ttcttcaagt atgtaaggat cagcgtcttt
     1261 cccaatatgc acaaattcaa tgtcaaggag attagcaaag tcacggagaa attcaagtga
     1321 taggtcatac actagtgtgg tgtgatgtgc cccgcccgct actaaccaac aagctgctga
     1381 cttttctaaa tttggttctg gtcgccacat aaggcgcgct acaggtaagt ttggcattga
     1441 tttagttggg gcaacacatt caacgtcaag agcaataagc cggaaacgat gacctaaatc
     1501 aattagtgag attgctttag ctttaccagg cttaccttca aagacaagtc gcgccggtgc
     1561 ttttttgcca ccaatacctt agtgatggac ttcaattttc ggtctttcac gagcaattga
     1621 cggacatact tcaagcatgt gtgcgcctaa tattagccct tctttcc
//
LOCUS       DAOPCD010000208         1666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_58010_length_1666_cov_2.756052, whole genome shotgun sequence.
ACCESSION   DAOPCD010000208 DAOPCD010000000
VERSION     DAOPCD010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58010_length_1666_cov_2.756052"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..404
                     /locus_tag="PLQ55_04705"
     CDS             240..404
                     /locus_tag="PLQ55_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29265.1"
                     /translation="MAFFAERKYYVVTCKFGHVGRDKYLPLDLPIRATSKKEASAKAK
                     KTGGGKARSP"
     gene            382..696
                     /locus_tag="PLQ55_04710"
     CDS             382..696
                     /locus_tag="PLQ55_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29266.1"
                     /translation="MVKRDHPDWCLKGPTEITKEEYNELKAKLVNDPYWNKKTRQNTA
                     LFADKLIDEPNYTNRRGIKTNTATFKKPTTAEIKMFHQKKRKIRDKEIQEFYDEANDY
                     ES"
     gene            complement(751..1317)
                     /locus_tag="PLQ55_04715"
     CDS             complement(751..1317)
                     /locus_tag="PLQ55_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29267.1"
                     /translation="MKRIHYYIVGTIILFATVIFSNSVGATSGANKRMEKLEVKARDL
                     FVTLSERYTKIEELHDNLSALDETATALVNNIEERITIFNDNKLLSQTVWPALQDAKY
                     IDENFMTLILYLKDNKDTFGSTLPSSFYTEFDAITSVLLPKINAYNSVTADYNTHIDL
                     FPNRLYLKGRNPYIIFDLALYPENLPLF"
     gene            complement(1332..>1666)
                     /locus_tag="PLQ55_04720"
     CDS             complement(1332..>1666)
                     /locus_tag="PLQ55_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29268.1"
                     /translation="TDFENKLIQFDVNTNGKDHLFFISQKTHHYEGKDVEGITLSPIG
                     TYDPGEPLNTYQDLINHLKNDANIMAIAAARAVQVEENVTITVTISIDGREVAVNNYY
                     FVLVVERS"
BASE COUNT          579 a          267 c          282 g          538 t
ORIGIN      
        1 aagaaattac gttttccttt atgcataatc gttcctacct tctttgtaca gattagtttt
       61 tgtgaagtag taagtaaatt aataagtaat ttacttttta attaaaattt aattcatcac
      121 acttatcttt aatattacca tattaaacaa atatcacacc ttaaatttag catgtgtatt
      181 caaaaatacg cctattagtg ttagaataag agtgaattta atattagtta ggagtaatta
      241 tggccttttt tgcggagcgc aaatattatg ttgttacctg taaatttggt cacgttggtc
      301 gtgataagta tttaccactt gatttaccta ttagagcaac aagtaaaaaa gaagcaagcg
      361 ctaaagcaaa aaagactggg ggtggtaaag cgcgatcacc ctgattggtg tttaaaagga
      421 ccaacggaaa ttactaaaga agaatataat gaacttaaag caaaattagt aaatgatcct
      481 tattggaata agaaaacacg gcaaaacacc gctctttttg ctgataagct cattgatgaa
      541 cctaattata cgaataggcg cggcattaaa acaaatacag ctacctttaa aaaaccaaca
      601 accgctgaaa taaaaatgtt tcatcaaaaa aagcgcaaga ttcgtgataa agaaatccaa
      661 gaattttatg atgaagcaaa tgattacgaa agttaaaaaa taaaatccac ctagtctatt
      721 agtaaggaca ctaaaaggtg ggtttttttg ttagaaaagt ggtaaatttt ctggataaag
      781 cgctaaatca aagataatat acggatttcg tccttttaag tagagtcggt tcgggaaaag
      841 atcaatatgt gtgttgtaat cagcagtcac actattataa gcattaatct ttggtaataa
      901 aacacttgtt attgcatcaa actctgtgta gaaggaagaa ggaagggtac taccaaaagt
      961 atccttatta tcctttaaat aaagtatgag tgtcataaag ttttcatcaa tatacttagc
     1021 atcttgcaat gcaggccaca cagtttgaga gagaagctta ttgtcattaa aaatagtaat
     1081 ccgctcctca atgttattta ctaaagcagt agcggtttca tctaaagcgg ataaattatc
     1141 atgtaattct tctatttttg tgtagcgctc acttaaggta acaaataagt cgcgcgcttt
     1201 tacttcaagt ttttccatgc gtttatttgc tccgctcgta gcgccaacgg agttactaaa
     1261 aataactgtc gcaaaaagaa taattgttcc aactatataa taatgaattc gcttcattta
     1321 atcgtctctt tctaacttcg ctcaacaact aagacaaagt agtaattatt gacagcaact
     1381 tcgcggccat caatcgatat tgtaacagtt attgttacat tttcctcaac ttgtactgct
     1441 ctcgctgcgg caattgccat gatattagca tcatttttca agtgattaat taagtcttgg
     1501 taagtgttaa gtggttcacc tggatcataa gtgccaattg ggctaagtgt aatcccttca
     1561 acatctttac cttcatagtg atgggttttt tgtgagatga agaacaaatg atctttacca
     1621 tttgtattaa catcaaactg aatgagctta ttttcaaagt cagtat
//
LOCUS       DAOPCD010000209         1664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_58103_length_1664_cov_2.822250, whole genome shotgun sequence.
ACCESSION   DAOPCD010000209 DAOPCD010000000
VERSION     DAOPCD010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58103_length_1664_cov_2.822250"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PLQ55_04725"
     CDS             <1..761
                     /locus_tag="PLQ55_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29269.1"
                     /translation="QKISFKFDLYTIIIGALLGLSFVMSLVHNIIRQMGFIAFEFWLL
                     RYAFLALLFCIFLVARALKHKELMLITTTLIVAHYLIFPVGTVSFNGFLDFEIQTQII
                     VALVLIALILLIVASFIKVDIYEKVETFYKYVVAVLFALIGFVFLLDTFINGFRDYGD
                     FRWTRAFFALSGVFYYAALVGLFALPILDTGILTPKKKETVQEVEVEVKSEEEKPKEA
                     EKVEAAPKKTEVKEPKVKVEKETEKAEPEAEPKE"
     gene            966..>1664
                     /locus_tag="PLQ55_04730"
     CDS             966..>1664
                     /locus_tag="PLQ55_04730"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HPK29270.1"
                     /translation="MNQDFIYGADISSLLAVEELGGTFTSDSGEKQELFEILKSNGFN
                     LARIRLFVDPKSNKGVSYGAGHNDLETALTLAKRAKDHDFKVLLNLHYSDFWADENHQ
                     TLPKAWAKLDFADLEYQVYAYTKEVVTTFKEENITLDYIQVGNEVNKGILWPHGKLFR
                     RKKATQTEYDALAILLNAGIKAIREVTPNVKIMIHLGQGSNPEMYADFLHFMEPRLFK
                     FDAIGLSYYPYIHRQ"
BASE COUNT          540 a          244 c          317 g          563 t
ORIGIN      
        1 cacaaaagat ttcatttaaa tttgacttat atacgattat tattggtgca ttacttggct
       61 tatcatttgt catgtcactt gtgcataata tcataagaca aatgggcttt attgcttttg
      121 aattttggct actgcgttat gcttttttag cattactatt ttgtatcttt ttagtggcaa
      181 gagcgcttaa acacaaagag ttaatgctta ttacaacaac tttaattgtg gcacattatt
      241 taatctttcc ggttgggacc gtttctttta atggtttcct tgactttgaa atccaaaccc
      301 aaattattgt agcgcttgtc ctaattgctt taattctttt aattgtcgct tcctttatta
      361 aagttgatat ttatgaaaaa gttgaaacat tttataaata tgtggtcgcg gtattattcg
      421 ccttaattgg ctttgtcttt ttactcgata cctttattaa tggctttaga gattatggtg
      481 acttccgctg gacaagagcg ttctttgctt taagcggtgt cttttattac gctgctttag
      541 tgggattatt cgcgcttcca attcttgata ctggtatttt aacgccaaag aaaaaagaaa
      601 cagtgcaaga ggttgaagta gaagttaaaa gtgaagaaga aaaaccaaaa gaagcagaaa
      661 aagtagaagc tgctccaaaa aagacagaag taaaagaacc gaaagtaaag gttgaaaaag
      721 aaacagaaaa agcagaacct gaagcagaac caaaagagta atagttttac ttttattaaa
      781 tttatatgct actataaaaa tacaccttgg gcggcactag gcaggaacca aataatagct
      841 caaggtgggc gacactactt gttagtgtcg ttttttttat tactatgttg tgacttttaa
      901 tgaaagttaa gcattagtca tttataatta tagtgttgta ttgcttagtt ttaggaggga
      961 agataatgaa tcaagatttc atttatggtg cggatatctc ttcactttta gcagtcgaag
     1021 agttaggtgg tacttttacg agtgatagtg gtgaaaagca agaacttttt gaaatattaa
     1081 agagtaacgg ctttaattta gcgcggattc gcttatttgt cgatcctaaa tcaaataaag
     1141 gtgtttctta tggtgcgggt cataatgatt tagagacggc attaacactt gcaaaaagag
     1201 cgaaggacca tgattttaaa gtattactta atcttcatta tagtgatttt tgggcagacg
     1261 aaaatcatca aacattaccc aaagcatggg ctaaactaga ctttgcggat cttgaatatc
     1321 aagtctatgc ctacactaaa gaagttgtta ctacttttaa agaagaaaat ataacacttg
     1381 attatattca agttggtaac gaagttaata aaggaatctt atggccgcat ggtaagcttt
     1441 tccgtcgtaa aaaggcaaca caaacagaat atgacgcttt agcgatttta cttaatgctg
     1501 ggattaaagc aattcgtgaa gtaacaccga atgtgaaaat aatgattcat ttagggcaag
     1561 gttctaatcc agagatgtac gcagatttct tacattttat ggaaccaagg ctttttaaat
     1621 ttgatgctat tggattatct tattatccgt atattcaccg ccaa
//
LOCUS       DAOPCD010000210         1645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_59100_length_1645_cov_3.037107, whole genome shotgun sequence.
ACCESSION   DAOPCD010000210 DAOPCD010000000
VERSION     DAOPCD010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59100_length_1645_cov_3.037107"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..>1645
                     /gene="leuS"
                     /locus_tag="PLQ55_04735"
     CDS             10..>1645
                     /gene="leuS"
                     /locus_tag="PLQ55_04735"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPK29271.1"
                     /translation="MAYDFRAAEKKWQKYWEENNTFYTDMYDFSKPKYYVLDMFPYPS
                     GDGLHVGHVEGYTATDIVARFKRMQGYNVLHPIGFDAFGLPAEQYAIRTNNHPAIFTN
                     ENIANFRRQLKSLGFSYDFSKELSTSEPKYYKWTQWIFTELFKSDLAYVDNIPVNFCP
                     ELGTVLANEEVIDGKSEVGGFPVIRKPMRQWLLRITEYAERLLSDLDGLDWPASTLDM
                     QRNWIGKSEGTTITFKVANTNYEFDVFTTRVDTLYGATYACLAPEHPLVLKVVSDTQK
                     EEVKRYIESASHKSDLERTELNKDKSGVYLGVNAINPLNNKEIPLYVADYVLGSYGEG
                     AVMAVPAHDERDYEFAKRHGLPMVQVLAGDISENAFIDDAPHINSPLINGLNIEESKK
                     VLNEHLTNKGVGRVTTNYKLRDWLFSRQRYWGEPIPIMIKEDNTFIPVPLSDLPLTLP
                     PLKEYKPSGTGESPLALASSWINTTYEGEKVKRETNTMPQWAGSCWYYIRYLDPHNDE
                     KIADPELIKHWLPVDLYIGGAEHAVLHLLYARFWHKF"
BASE COUNT          499 a          284 c          373 g          489 t
ORIGIN      
        1 ggtaacatta tggcttatga ttttcgtgcc gcagaaaaga agtggcaaaa atattgggaa
       61 gaaaataaca cattttatac agatatgtat gactttagta aaccaaagta ttatgtgtta
      121 gatatgtttc cctatccttc aggcgacgga ctgcatgttg gtcacgttga agggtatacc
      181 gcgactgata tcgtcgctcg ctttaaacgg atgcaaggct ataatgtctt acacccgatt
      241 ggatttgacg cctttggttt accagcggaa caatatgcga ttcgcacgaa taatcatccg
      301 gctattttta ctaacgaaaa tatcgctaat tttagacggc aattaaagtc gcttggcttt
      361 agttatgatt ttagtaaaga actttctact agtgaaccaa agtattataa atggacacag
      421 tggattttca ctgaactttt taaaagcgac ttagcctatg tcgataatat ccctgttaac
      481 ttttgtcctg aactaggaac agtactcgca aatgaagaag taattgacgg taaaagtgaa
      541 gttggcggat tcccggtgat tcgtaaaccg atgcgccagt ggttattacg cattacggag
      601 tacgcggagc ggttattaag tgatttagac ggtttagatt ggcccgcttc tacgcttgat
      661 atgcaacgta actggattgg gaagagcgaa ggcacaacga ttacttttaa agtagcgaat
      721 acaaattacg agtttgatgt ctttacaacg cgtgttgata cgctttatgg ggcgacttat
      781 gcatgtttag cgcctgaaca tccgcttgtg ttaaaagttg ttagtgacac gcaaaaagaa
      841 gaagtaaagc gttatattga aagcgctagt cataagagcg acttagaacg gacagaactt
      901 aataaagata aatcaggggt gtatttagga gttaacgcta ttaatccgct taataacaaa
      961 gaaatcccgc tttatgttgc tgattatgtt ttagggtcat atggtgaagg cgcagtaatg
     1021 gcggttccag cgcatgacga acgtgattac gaatttgcta agcgccatgg cttaccgatg
     1081 gttcaagtgt tagcaggcga cattagtgaa aatgctttta ttgatgacgc cccccatatt
     1141 aatagtccgc ttattaacgg gcttaatatt gaagagagta aaaaagtact taatgaacac
     1201 ttaacgaata aaggagtagg tagagtaacg actaattata aattacggga ctggttattc
     1261 tcacgtcaac gctactgggg tgaaccaatt ccaattatga ttaaagaaga taatactttt
     1321 atccctgtac ctttaagtga tttaccgctt actttaccgc cgttaaagga atataaacct
     1381 agcggtactg gtgagtcgcc acttgcgctt gcaagtagtt ggattaatac aacttatgag
     1441 ggtgaaaaag ttaaacgcga aacaaatacg atgccgcaat gggcggggtc atgctggtat
     1501 tatattcgtt atttagatcc gcataatgat gaaaaaattg ctgatccaga acttattaag
     1561 cactggctcc ctgttgatct ttatattggt ggggcggaac acgcggtctt gcatttactt
     1621 tatgcgcgtt tttggcataa gtttt
//
LOCUS       DAOPCD010000211         1639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_59457_length_1639_cov_2.097222, whole genome shotgun sequence.
ACCESSION   DAOPCD010000211 DAOPCD010000000
VERSION     DAOPCD010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59457_length_1639_cov_2.097222"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..588
                     /locus_tag="PLQ55_04740"
     CDS             103..588
                     /locus_tag="PLQ55_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29272.1"
                     /translation="MITFEKSKRYLFYYVGVLLVTITLFISWVVMIVKNPGSTSGDIV
                     LLSFLFLILFGGEALFLIIYLIRPKVVLSVDEEGAYLHLSKKKTKEVAFSNFSSVGNM
                     RRNLVIATKDSELYVVRFLKDHKKAEETLKTMFNTFITNHPERYFDMTESADKQKHEE
                     K"
     gene            626..1066
                     /locus_tag="PLQ55_04745"
     CDS             626..1066
                     /locus_tag="PLQ55_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29273.1"
                     /translation="MQFNQDFDKIIGEDIYLSITEKNSGLIGGLPYYKYGIHLNDGTL
                     VGNILVRIGHNFNSYYSGNFVYQVSGAYRGQNLAGKALNAILVVAKYHKMEYVIFSAP
                     SSSKGYLRTFEKMGAKLLEIVTPPENYMYYYKGIEPLAIYRLDL"
     gene            1085..1486
                     /locus_tag="PLQ55_04750"
     CDS             1085..1486
                     /locus_tag="PLQ55_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29274.1"
                     /translation="MSDTAEFVKVEVIDEENKEGMSRRKKFEIRKLVMSTYVTLVVIA
                     YLAMVFFLDKWHPHWVIFLSIPVVDSLVTAILEKKIALFSVDALIILAFVVVGAVTGK
                     WHPAWMILLLVPLWRYILNTIKRIKRIKAMD"
BASE COUNT          524 a          234 c          318 g          563 t
ORIGIN      
        1 tgaagcgatg gtcaaagctg gcgctaatcg aattggcacg agtaatggtg ttgctttctt
       61 tactggtaaa aaaggtaccg gttactaaaa aacacttgat ttatgattac ttttgaaaaa
      121 tctaaacgat acttatttta ttatgtcggg gttttacttg taacaattac tttgtttatt
      181 tcgtgggtcg tgatgatcgt taaaaaccct ggtagcacaa gtggtgatat tgttttacta
      241 tcattccttt tccttatttt atttggtggc gaagcattgt ttttaattat ttacctcatt
      301 cgtcctaaag tagtactcag tgttgatgaa gaaggcgctt acttacattt aagtaagaaa
      361 aagacaaaag aagtagcgtt tagtaatttt agctctgttg ggaatatgcg ccgtaattta
      421 gtgattgcaa cgaaagatag tgaactttat gttgtgcgct tcttaaaaga tcataaaaag
      481 gctgaagaaa cgcttaaaac aatgtttaat acttttatca ctaatcatcc agaacgttat
      541 tttgatatga ctgaaagtgc ggataagcaa aagcacgaag aaaaataatc attccgttta
      601 taatgaaaca ggaggaatga taaatatgca gtttaatcaa gactttgaca agattattgg
      661 tgaagacatc tatttaagta ttactgagaa aaatagtggc ttaattggtg gtttacctta
      721 ttataaatat ggtattcatt taaatgacgg taccttagtc gggaacatct tagtgcgcat
      781 cggccataat tttaattctt actacagtgg gaactttgtt tatcaagtta gcggagcgta
      841 tcgcggacaa aatcttgcgg gtaaagcatt aaacgcaatt cttgttgtcg caaagtacca
      901 caagatggaa tacgttatct ttagtgcgcc aagttcgagc aagggttatt tacgcacttt
      961 tgagaaaatg ggtgcaaagt tattagaaat tgtcacacca cctgaaaatt atatgtatta
     1021 ttataaaggt atagagcccc tcgcgatata tcgcctagat ttataaaaag agaggaacac
     1081 atttatgagt gatacagctg aatttgttaa agttgaagtg attgatgaag aaaataaaga
     1141 gggaatgagt cgacgcaaga aatttgaaat tagaaaatta gtaatgtcga catatgtgac
     1201 gcttgtcgtt attgcttacc tcgcaatggt ttttttcctt gataaatggc atccacattg
     1261 ggttattttc ttatcaattc ctgtcgttga ttcattagtg acagccattt tagaaaagaa
     1321 aatcgcctta ttttccgttg atgctttaat tatcctcgcc tttgtcgtag ttggtgcggt
     1381 aacgggtaaa tggcatccag cgtggatgat cctattactt gtgccattat ggcgttatat
     1441 tttaaatacg attaaacgca ttaaacgtat taaagcaatg gattaagttt aattgttaga
     1501 taaattttaa cttatctaat tgtggtatca ataattttac tggtttatat taacctaaat
     1561 tatctttgaa aaatttagtt cttattgcac tagtcaacta aatgtagaca ctttaataac
     1621 ttgatagtca ctacttttg
//
LOCUS       DAOPCD010000212         1638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_59487_length_1638_cov_2.835123, whole genome shotgun sequence.
ACCESSION   DAOPCD010000212 DAOPCD010000000
VERSION     DAOPCD010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59487_length_1638_cov_2.835123"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1169)
                     /locus_tag="PLQ55_04755"
                     /pseudo
     CDS             complement(1..1169)
                     /locus_tag="PLQ55_04755"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
     gene            complement(1437..>1638)
                     /locus_tag="PLQ55_04760"
     CDS             complement(1437..>1638)
                     /locus_tag="PLQ55_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPK29275.1"
                     /translation="IGVAEYRHNNGFYDAEDAVNIADVAMYVSKSTGRGRVTYFNEEQ
                     ARKVMASGPLEKTLELLESRAR"
BASE COUNT          503 a          332 c          248 g          555 t
ORIGIN      
        1 cgattaatcg ggtaaacata ttcttcaact gctgggtgac ttattttaat gggtttacct
       61 aaaccttcac tcaagtgtcc cactaatgtg aatgtcttcg ttcttttatt aactttaagt
      121 acataaaggt gattaataac tacttgtgac catgtactaa tataattttc ttcgtcaaat
      181 agccagctta cgccaatatt cgtcgtagcg actcggagat gcccgttata gtaatccata
      241 gaaaattgat taagcatcga gccatcaaca attgcggcag cgacataatt aatatttgcc
      301 ttatctaaat caagttcaaa ttgactaatt gttaaagttg aataatagct attttccgta
      361 aataaccagt tagagtcaca tagatataga tcagtttgtg aaacatacac ttgcttatat
      421 ccataattag cacctaaaaa tcctttagcg ttaaaagtaa tcgcctgcgg gtcagcggct
      481 aatttaagtc caattatctt tgtcattccg tgcaccggtg tatcttcaaa ataataaata
      541 tcgttatatc caatattagt ttcagtgcca tctaataaac ttatttccgg acgcggatct
      601 tcttcatcaa tcgtaatata ttcataacca acaaagaata agttattatc aattacacgg
      661 tggtcgataa taatattgtc cttaatattt agttcataca ctacttgatg attcgtccgt
      721 tcaataacag taattgctgt cgtggcatac ccaaaccatc ctggcagcca aaactcttca
      781 tattctggaa gtaaaacata ttcatacttt tcactgataa ctacgaccta ttccggtagc
      841 aaatacatcc caataccgta cgaatccgca aaagtgagtg atttacttaa tgtaatgctt
      901 ttatcatcag caacatcaaa tacttgaagc gcgccgcgtt cacgcgctaa ataataaatt
      961 tgatagccat cagttttaat aatgtcagct tcactaacac ctgccacttg ttcgtttgta
     1021 ccagtgaaat cgcgaccttt cgtcccagcg cccgtatcag attctggaac agcatcaccc
     1081 ccagcgattg tatcgtaata ttcattcgtg gacttgttac cttctttgac taattcatta
     1141 aatttttgtt tcgtttttac ttgttgcatt ttcgttttgg taattaagtc gctattagtt
     1201 taacaactcg ctaaacttgt taacattccc aaaaagaata gtgatactat aaaccgtgaa
     1261 agttttggtt taaacgattt tttcatatta ttttcctttc agtaattaag tggtatacac
     1321 ttaactcgcg tttaaaacgg attactatct actattattt tgcatcgttt ttgaattttc
     1381 gtcaatatta ttaaaaaaga cactcgaagt gtctttaatt actagttaac agattattat
     1441 cgcgcgcgtg attcgagtaa ttctaaagtc ttttctaaag gtccagaggc cataactttt
     1501 cgcgcttgtt cttcattaaa atatgtcaca cggccgcgac cagtggattt tgatacatac
     1561 atggcaacat cagcaatatt gacagcatct tcagcgtcat aaaacccgtt attatgacga
     1621 tattccgcaa caccaatt
//
LOCUS       DAOPCD010000213         1633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_59749_length_1633_cov_2.796578, whole genome shotgun sequence.
ACCESSION   DAOPCD010000213 DAOPCD010000000
VERSION     DAOPCD010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59749_length_1633_cov_2.796578"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>993)
                     /locus_tag="PLQ55_04765"
     CDS             complement(<1..>993)
                     /locus_tag="PLQ55_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPK29276.1"
                     /translation="DIIGRQAKKDLRQKVYNKIVRLGVRTTDGMKVAGLTQVSLEGVE
                     QLDLYYSTYIPQFFYALLAPIILFFVTVWFDWRFSLVLLAAVPLIPLSIIAISKYAKR
                     IFAKYWGKYTAMGDKFLDSIQGLRELKIFKADAYQQTKMNEASEEFRVITMKVLVMQL
                     ASTTIMDIVAYTGAGIGVLIAIISVLKWGLSPFSALFLILVAVEFFLPLRALGSAFHV
                     AMNGASAGQKILSLLAEEETPWGTKEVTNANIEIKDVTFAYNGHANVLNDVSMTFKEQ
                     GMVSIVGESGSGKSTIVNLITGALRPQFGSVSVGGELLETLSRESYYSNLAVISY"
     assembly_gap    994..1003
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1004..1251)
                     /locus_tag="PLQ55_04770"
     CDS             complement(<1004..1251)
                     /locus_tag="PLQ55_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29277.1"
                     /translation="MINRKLLKLIDNHKHIIFTVILMVCGLITNIVITGSLVYLIKEV
                     ANHSDDSSVWTYLWPALIAIISIIIRYLLSISVGYLTD"
BASE COUNT          545 a          313 c          238 g          527 t
ORIGIN      
        1 gtaactaatg accgcaagat tactataata actttcacgc gacagagttt ctaataattc
       61 gccaccaaca ctcacactcc caaattgcgg tctaagtgca cctgtaataa gattaacaat
      121 tgttgatttt ccacttcctg attcaccaac aatcgaaacc atgccttgct ctttaaaagt
      181 cattgataca tcatttaaca cgttagcgtg accgttatac gcaaaagtta catctttaat
      241 ttcgatattt gcattcgtta cttcttttgt accccatggc gtttcttctt ctgcaagcag
      301 tgataatatc ttttgaccag cacttgcccc gttcattgca acgtgaaacg ccgagccaag
      361 cgcacgaagc ggtaagaaaa actcgactgc gactaaaatt aaaaataacg cactaaatgg
      421 tgataatccc cattttaaaa cactaattat tgctattaat acaccaattc cagcgccagt
      481 ataagcgaca atatccataa tcgttgtact cgctaattgc atgactaaca ctttcatagt
      541 aatcacacga aattcttcac ttgcttcatt catctttgtt tgctgataag catcggcctt
      601 aaagatttta agttcgcgta atccttggat actatcgaga aatttatcac ccattgctgt
      661 gtattttccc cagtatttag caaagatccg ttttgcgtat ttagatatag cgataatgga
      721 aagtggaatt aggggtaccg ctgctagtag cacgagtgaa aaccgccaat caaaccatac
      781 cgttacaaaa aacaaaatta ttggcgctaa tagtgcgtag aaaaattgtg gaatataagt
      841 tgagtaatat aaatctaatt gctccacgcc ttcaagtgaa acttgcgtta aaccagccac
      901 cttcatcccg tcagtagttc tcacgccaag tcttacaatc ttgttataca ctttttgacg
      961 caggtctttt tttgcttgac gaccaataat atcnnnnnnn nnnatatctg tcaagtaacc
     1021 aacactaatt gaaagtaaat aacgaataat aattgaaata atagcaatta atgcaggcca
     1081 taaataagtc catacactac tatcatcact atgattagcg acttctttta ttaaataaac
     1141 aagcgatcca gttattacaa tatttgtaat taatccgcat accatgagta tcacagtgaa
     1201 aataatatgt ttgtgattat caattaattt cagtaacttt cgattaatca tttatgcttt
     1261 ataactccta attacttaag cttattagtt agcgagcgct aacctttatg gtaaaaaaga
     1321 aggcggcgat attcttgtta gtcgccgcta acttcgtact aactatacat tccatcttgt
     1381 caattgtcaa tgataactat aatattcatc cttgagtttt gtctgtaaat tacaataaaa
     1441 gtaatgatac gagcattata aaaagatact attattaagt cactaatgta gaatacacta
     1501 ctttattaga attattgaag ttcacatcat tgatttttca gtagtgttat gttattttga
     1561 atgtagagaa tttatattta aggagattaa ctattcttaa tcaagttaaa tagtaaatat
     1621 atatcaaaaa gat
//
LOCUS       DAOPCD010000214         1621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_60393_length_1621_cov_2.345466, whole genome shotgun sequence.
ACCESSION   DAOPCD010000214 DAOPCD010000000
VERSION     DAOPCD010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60393_length_1621_cov_2.345466"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLQ55_04775"
     CDS             <1..316
                     /locus_tag="PLQ55_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29278.1"
                     /translation="NLNEVGLTAEALAPYQSRLVTGELSVKTVAKKDADNNISIVLTN
                     GTDDITLRLDSRLPKFAEFEFLMDLAVGDVVVLDGALIGWYNVPQLAADDPVQVVKKV
                     AP"
     gene            439..987
                     /locus_tag="PLQ55_04780"
     CDS             439..987
                     /locus_tag="PLQ55_04780"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPK29279.1"
                     /translation="MNRNQIEKVLMKDEKVLYTYKPMFIKTVIITVICYLFTLLFVLL
                     ALLIPASAESMEINVTGGIVALAVIGITVFFGVVLLLLALAYRNKFYAVTNKRFIIQS
                     GLLGIDFSSIPISGVKFISVNVSVLDKILGKGTGTVSFGTVSTPVTPGQGAKFNFLNI
                     YDVYENYRIFKELSDKAQTEDK"
     gene            1116..>1621
                     /locus_tag="PLQ55_04785"
     CDS             1116..>1621
                     /locus_tag="PLQ55_04785"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPK29280.1"
                     /translation="MEKKSHNLPMPKVHSWQDVKDVYFLYITNAEDRANIEEAYNFAL
                     QKHEGQFRKSGHPYITHLIEVAFILAELTAGPSTIIAGLLHDVVEDTDVTVSEIDKMF
                     GKDVAMIVDSVTKIQRLKLSKKVDDSDFVYEDHRKIFLGMAKDIRVIIIKLADRLHNL
                     RTLYFLSH"
BASE COUNT          543 a          256 c          296 g          526 t
ORIGIN      
        1 taacttaaat gaagttggtc ttaccgctga agctttagca ccatatcaat cacggttagt
       61 tactggtgaa ttatcagtaa aaaccgttgc caaaaaagat gcagataaca atattagcat
      121 tgtattaaca aatggaactg atgatattac cctacggctt gatagtcgtt tgccaaaatt
      181 tgctgaattt gaattcttaa tggatttagc ggttggtgat gtcgtcgtct tagatggcgc
      241 ccttatcggt tggtataatg ttccacaatt agccgctgat gatccagtgc aagttgtgaa
      301 aaaagttgct ccataattaa taattaatta ttaaatatac gactcacggt gtccccgtga
      361 gtcttttctt tactatttct ttacaagtaa cataaattta agcatggtaa aattaaaatg
      421 agaaaggaga acttaattat gaaccgcaat caaattgaaa aagtattaat gaaagacgaa
      481 aaagtattat atacttataa gccaatgttc attaaaacag taattattac cgttatttgt
      541 tatttattca cgcttttatt cgtcttactc gctttactta ttccagcatc agctgaaagc
      601 atggaaataa atgtaactgg gggaatagtg gcacttgccg ttattggtat tacagtattc
      661 tttggtgtcg tattactact tttagctcta gcgtaccgta ataagtttta tgccgtcacg
      721 aataaacgct ttattattca aagcgggcta cttggcattg atttttcaag tattccaata
      781 agtggtgtca aatttattag tgtcaatgtt agtgtgctcg ataaaatact cggtaaaggt
      841 actggtactg tgtcttttgg tactgttagc acaccagtaa caccaggcca aggcgctaaa
      901 tttaatttct taaatattta tgatgtctat gaaaattatc gaatctttaa agaattaagc
      961 gataaagccc aaaccgaaga taagtaatta gaaatattac ttacgaactc gactctcaac
     1021 agagagtctt tttttattaa aaacacctta ttaagtaaag cataatcaaa gcgattatta
     1081 atttgttata attaataaga aaggaaacgc atagtatgga gaaaaaaagt cataacctac
     1141 caatgccaaa agtccattcg tggcaagacg tcaaagatgt ctattttctc tatattacta
     1201 atgcagaaga ccgcgctaat attgaagaag catataattt tgcgcttcaa aaacatgaag
     1261 ggcaatttcg taaaagcggt catccttata ttacgcattt aattgaggtt gcctttattc
     1321 ttgctgaact aacagcgggt ccaagtacga ttattgctgg tttactgcat gatgtcgttg
     1381 aagacactga tgtaacagtt agtgagattg ataaaatgtt tggcaaagat gtggcgatga
     1441 ttgtcgatag cgtcactaaa attcaacgcc taaaacttag taaaaaagtt gatgatagtg
     1501 attttgtgta tgaagatcac cgtaaaatat tcttagggat ggcaaaagac atccgtgtta
     1561 tcattattaa acttgcggac agattacata atctgcgaac attatacttt ttaagccatg
     1621 a
//
LOCUS       DAOPCD010000215         1597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_61700_length_1597_cov_2.810636, whole genome shotgun sequence.
ACCESSION   DAOPCD010000215 DAOPCD010000000
VERSION     DAOPCD010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61700_length_1597_cov_2.810636"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLQ55_04790"
     CDS             <1..197
                     /locus_tag="PLQ55_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29281.1"
                     /translation="AKGSAKASFLRALGLTQEQNKLFLLGFVKTDQVPAIFSVLEYKY
                     KFKEPNSGFAFTFPLEKISC"
     gene            210..518
                     /locus_tag="PLQ55_04795"
     CDS             210..518
                     /locus_tag="PLQ55_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPK29282.1"
                     /translation="MTESMKALFIIVNAGFSEQIVEVVQNHGARGATIIPARGTGKKF
                     VKVLGIQYEPEREILLSVVEEDIALKITADVKENYGKNTPTNGLCFILPVADYMFKNE
                     "
     gene            570..1586
                     /locus_tag="PLQ55_04800"
     CDS             570..1586
                     /locus_tag="PLQ55_04800"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPK29283.1"
                     /translation="MGNFIRKHIDTLLLVLNVLVFVLFTLVYPFVTFKHTFLNALFYI
                     LLSAPLITFLVLRFLKYDRKTEESIFYYRRGFNLLALINNYLFMMSAESIFTLIKGHR
                     APLFIIYLIFFALMWLINIFALILELGYLDQLLPLREYATDTAKEETIALAKAAKFKR
                     FEEKVSTMYFFIALVAVLAVIFFIYPLVTEYFTIPLGGDFTQQQIPFYTNGYDDWWRF
                     LKTGEFPLWDSNTFLGVNNIGSNSFYYSMNPFFLPILIFPRELIPQGISILMIGKFVL
                     AAVTMRLYLKYMGVNEKEARFFAVIYAFSGWNTYYLWFNHFMEVAVIFPLIFLGIDTV
                     GKSA"
BASE COUNT          465 a          272 c          295 g          565 t
ORIGIN      
        1 atgcgaaggg gagcgctaaa gctagtttct tacgggcact tggtctaact caagaacaaa
       61 ataagctctt tttgctagga tttgtcaaga ctgatcaagt tccagcaatc tttagtgtat
      121 tagagtataa atacaaattt aaagaaccaa attctgggtt tgcttttaca ttcccacttg
      181 aaaagataag ttgctaggag gacattctta tgactgaaag tatgaaagct ttatttatta
      241 tagttaacgc gggctttagt gaacaaatcg ttgaagtcgt gcaaaatcat ggcgctagag
      301 gtgctactat tatccccgcg cgtggcactg gcaagaagtt tgtcaaagta cttggtattc
      361 aatatgaacc agagcgcgaa atcttactta gtgtcgtgga agaagatatc gcgcttaaga
      421 ttactgctga tgtgaaagaa aactacggta aaaacacacc aactaatggg ttatgcttta
      481 ttttgcctgt tgctgattac atgtttaaaa atgagtaaat aatagtttga taaccattaa
      541 tcttcaattt attttataat gataatacta tgggaaactt tattcgtaaa cacattgata
      601 ctttactcct agttttaaat gtcttagtct ttgttttatt tacgcttgtt tacccattcg
      661 tgaccttcaa acataccttc ttaaatgcac tattctatat tttacttagt gcaccgctta
      721 ttactttctt agtgcttagg ttccttaaat atgaccgcaa gactgaagaa agtatttttt
      781 attacaggcg tggcttcaac cttttagcgc ttattaataa ctatttattt atgatgagtg
      841 ctgagagtat ttttacgctt attaaaggtc atagagcgcc gttatttatt atttatttaa
      901 tattcttcgc tttaatgtgg ttaattaata tctttgcact aatccttgaa cttggttact
      961 tagatcaact tttaccgctt cgagaatatg cgactgacac cgcgaaagaa gaaacgatcg
     1021 cattagcgaa agctgcaaag tttaaacgct ttgaagaaaa agtttcaaca atgtacttct
     1081 ttattgcgct tgtggcagta ctcgcggtta ttttctttat ttatccactg gtgactgaat
     1141 actttacgat tcctttaggt ggtgacttta cacagcaaca aatccctttt tacacgaatg
     1201 ggtatgatga ctggtggcgc ttcttaaaga ccggtgaatt tccgctgtgg gatagtaata
     1261 cctttttagg tgttaataac attggttcaa atagttttta ctattcaatg aatcccttct
     1321 tcttaccaat tcttattttc ccgcgtgaac ttatcccaca agggattagt attttaatga
     1381 ttggtaagtt tgtactagcg gcagtaacaa tgcgtttata tttaaaatat atgggcgtta
     1441 atgaaaaaga agcacgattc tttgctgtaa tctatgcatt tagtggctgg aatacatatt
     1501 acttatggtt taaccacttc atggaagttg ccgttatttt cccgcttatt ttccttggta
     1561 ttgatactgt tggcaaaagc gcctgaatac gacatcg
//
LOCUS       DAOPCD010000216         1592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_61992_length_1592_cov_3.182173, whole genome shotgun sequence.
ACCESSION   DAOPCD010000216 DAOPCD010000000
VERSION     DAOPCD010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61992_length_1592_cov_3.182173"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLQ55_04805"
     CDS             <1..647
                     /locus_tag="PLQ55_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29284.1"
                     /translation="EEKKRLKIIGKPELIAYLLFNFFVIGVTVVTDNFFGIYLRTELG
                     VTTTQFGYIVAIAIAIEAGVMFLLALRKNNSFRVPAFSFFFIGTFLLIRPLVIVLNLS
                     PTLTMILSVFRGIAWGYYIVFNVRFLARVIPLKHLTKALLTGSIATTSSRIITSLIVG
                     ESLKHHSYQTVFFFSMFSVVFGMTISIIIAEIDRRKQKKVDMIVPFIGSDKDLN"
     gene            complement(644..1129)
                     /locus_tag="PLQ55_04810"
     CDS             complement(644..1129)
                     /locus_tag="PLQ55_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HPK29285.1"
                     /translation="MEIRDVTNVLFIKNNKVLLGYKKRGFGKKKYNGFGGKLKSGETI
                     EMAAIREAKEEAGLTMKTYYKAAEIDFAESYPLRMHLYVCTEWSGEITESDEMVPRWF
                     GFNEVPVDEMWDDDKYWLHLALSGQKFRASFVFENDDDVTGTAENKVISYSIKLVDTL
                     D"
     gene            complement(1266..1469)
                     /locus_tag="PLQ55_04815"
     CDS             complement(1266..1469)
                     /locus_tag="PLQ55_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29286.1"
                     /translation="MVKEFGLPWIAHLLLQFFIGPIWGAVIRLVRGRDLWAVIYLLTG
                     GFFVIGWIYDLVMLSIHRDYKLA"
BASE COUNT          500 a          290 c          260 g          542 t
ORIGIN      
        1 ctgaagaaaa aaagagactt aaaattattg gaaaacccga actaattgct tatttactct
       61 ttaacttctt cgtcattggg gttactgttg ttaccgataa cttttttggt atttatttaa
      121 ggactgaatt aggagttacg acaacacagt ttggatatat tgtggcgatt gcaatagcaa
      181 ttgaagcagg cgtgatgttt ttacttgcac tacgaaaaaa taatagtttc cgagtgccag
      241 ccttttcgtt tttctttatc ggaacatttt tgcttattcg tccgttagtg attgttctta
      301 atttatcgcc gacattgaca atgatattgt cagtctttcg cgggattgca tggggatatt
      361 acatcgtttt taatgtgcgc tttttggctc gtgttatccc cctaaagcac ttaacaaagg
      421 cacttcttac tggatcaatt gccacaactt caagtcgcat tattactagt ttaatcgttg
      481 gcgaaagtct taaacatcat tcttatcaaa cggtgttttt cttttcaatg ttctctgtag
      541 tttttgggat gacaattagt attattattg ccgaaattga tcggcgaaaa caaaaaaagg
      601 tcgatatgat cgtacctttt attggttcag ataaagattt aaattaatct agggtatcca
      661 ctaatttgat ggagtaactg atgactttgt tctcggcagt accggtaaca tcgtcatcat
      721 tttcaaaaac aaaactggcg cggaactttt gaccgctaag tgctagatgc aaccaatatt
      781 tatcatcatc ccacatttca tcaactggca cttcattaaa accaaaccat cttggtacca
      841 tttcatcaga ttcggtgatt tcaccgctcc attcagtaca tacatataaa tgcatccgta
      901 atggatagct ttccgcaaaa tcaatttcag cggctttata atatgttttc attgtaagac
      961 ccgcttcttc tttagcttct ctaatcgcag ccatttcaat tgtttcacca ctctttaatt
     1021 taccaccaaa gccgttatat ttctttttac caaaaccgcg ttttttataa ccaagtaaaa
     1081 ccttgttgtt ttttataaat aatacgtttg taacatctct aatttccata tttttttgtc
     1141 tcttttcaat aatagtgtgt taagcacatt gcttaatata cgctaataag ggggaaataa
     1201 caagaaaaag gaataaaaaa aacacaccag aggtgtgttc ctttaacttc actaatttta
     1261 attaattagg caagtttgta gtctctgtgg atgctaagca tcactaagtc gtaaatccag
     1321 ccaataacga aaaatccgcc agtgagtaga taaatgacgg cccaaaggtc acgtccgcga
     1381 actaagcgaa taactgctcc ccaaataggt ccaatgaaaa attggaggag taagtgagca
     1441 atccatggta atccaaattc tttaaccatt ttgtacctcc tatggtttat gattttatga
     1501 tatatcaatt aatataaaat ttcaatctaa taaatgtaaa taatagaaga aaaggttaaa
     1561 ttgacaagat aaaaaactga aagaatcatt tt
//
LOCUS       DAOPCD010000217         1582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_62561_length_1582_cov_2.952849, whole genome shotgun sequence.
ACCESSION   DAOPCD010000217 DAOPCD010000000
VERSION     DAOPCD010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62561_length_1582_cov_2.952849"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLQ55_04820"
     CDS             <1..772
                     /locus_tag="PLQ55_04820"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK29287.1"
                     /translation="IPYVLFMVLFVLLPLFFVAFYAFTNSAGRFSFEPMVNFFTSSAK
                     WRVLFVSFIFGILNTALCLVIGYPLAMILANRKYNTNAVLVMLFVMPMWINFIIRSWA
                     MRDVLVFLGIRGGEYPELATLLGLTYNYLPFTILPLYSTMLKMDKNQIEASYDLGAGP
                     VTTFFKVIVPMTMPGIISAATMVFMPTMTSYVIADILGEGKVTLFGKYIEIYFTQNIW
                     NEGSFLALVMLALIGISVLVPKLLTRKEAKVKGVGIYD"
     gene            765..>1582
                     /locus_tag="PLQ55_04825"
     CDS             765..>1582
                     /locus_tag="PLQ55_04825"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK29288.1"
                     /translation="MIKKILAKVYVYLILFLLYLPILFLIIYSFTPAETTGVWEGFSF
                     ELYGRVFLNEKIMRALLNTLIIALSSATVATILGTLGAIGVFYMKKRPQRIMEGINEI
                     PVVNAEIVVAISLAILFRVFANFLEARGVATNSPNFVTLLIGHVVLSISFVYLNVKPK
                     LQQMDPNDYEAALDLGAKPMYAIRKVVLPAIVPGIISGFMLAFTLSLDDFIITNFLRN
                     QSFDTLSTYVQGVIVRAAIPAEMRALTTYIVLVTLAVVLVSGIKRESRSSRKAML"
BASE COUNT          419 a          290 c          331 g          542 t
ORIGIN      
        1 gattccctat gtgctcttca tggtgctctt cgtcttatta ccgcttttct ttgtagcatt
       61 ttacgccttc actaacagtg cggggcgctt tagttttgag ccaatggtga acttctttac
      121 aagtagtgcg aaatggcgtg ttttattcgt ttcctttatt tttggcatat taaatacagc
      181 actatgctta gttatcggct atccgttagc gatgatttta gcgaatcgaa aatataacac
      241 taacgcagta ttagtgatgc tctttgttat gccaatgtgg attaacttca ttattcgctc
      301 atgggcgatg cgtgatgtac ttgtgttcct tgggattcgt ggcggtgagt atccagaact
      361 tgccaccctt ttagggttaa cttataacta tttacctttt accatcttgc cgctttattc
      421 aacgatgcta aaaatggata aaaatcaaat tgaagcaagt tatgacttag gcgctggccc
      481 agtaacaact ttctttaaag taattgtacc aatgacgatg cccgggatta ttagtgcagc
      541 gacaatggtc tttatgccaa cgatgacaag ttacgttatc gcggatattt taggagaagg
      601 taaagtgaca ttatttggga agtatattga aatttatttc acccaaaaca tttggaatga
      661 aggctcattc cttgcgcttg taatgcttgc gcttatcggt attagtgtcc ttgttcctaa
      721 actcttaaca agaaaagaag caaaagtgaa gggggtcggt atttatgatt aagaagattc
      781 tcgcaaaagt atatgtatac ttaattttat tccttttgta cttaccaatt ttatttctca
      841 ttatttatag ctttactccc gcggaaacaa caggagtatg ggaaggcttt agttttgagc
      901 tttatggacg cgtcttttta aatgaaaaga ttatgcgcgc cttacttaat acactgatta
      961 ttgcgcttag tagtgcaacg gttgcgacaa tcttaggcac tcttggggca attggtgtct
     1021 tttatatgaa aaagcgtccg caaagaataa tggaaggaat taatgaaata cctgttgtta
     1081 acgcggaaat cgttgtagcg attagtttag ctattttatt ccgggtgttc gctaatttct
     1141 tagaagcacg tggtgtagcg acaaatagtc caaactttgt cacactttta atagggcatg
     1201 ttgtgcttag tatttctttt gtgtatctca atgttaaacc taaactccaa caaatggatc
     1261 caaatgatta tgaagcagcg cttgacttag gtgcaaaacc gatgtacgcg attcgtaaag
     1321 tcgtgttacc cgcaattgta cctggtatta tcagtggctt tatgcttgct tttactttaa
     1381 gtttagatga ttttatcatt acaaacttct tacgtaatca gtcttttgac accctttcaa
     1441 cttatgtaca aggtgtgatt gtgcgggcag caattcccgc cgagatgcgc gccctaacga
     1501 cttatattgt gttagtcaca ctagcggttg tgcttgtgtc tgggattaaa cgcgagtcac
     1561 gctcttcgcg caaagcgatg ct
//
LOCUS       DAOPCD010000218         1570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_63268_length_1570_cov_2.776898, whole genome shotgun sequence.
ACCESSION   DAOPCD010000218 DAOPCD010000000
VERSION     DAOPCD010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63268_length_1570_cov_2.776898"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>237)
                     /locus_tag="PLQ55_04830"
     CDS             complement(<1..>237)
                     /locus_tag="PLQ55_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HPK29289.1"
                     /translation="SLESTHHIFSVRGPLSFMFGGYAFWDMQDLENPNLEQYQSAVES
                     VQNFIVNAITQYNLEKKDITLLGFSQGAVLVQSIA"
     assembly_gap    238..247
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            515..985
                     /gene="ybeY"
                     /locus_tag="PLQ55_04835"
     CDS             515..985
                     /gene="ybeY"
                     /locus_tag="PLQ55_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPK29290.1"
                     /translation="MMLKLTVEFYDDYAPTYKNYRAIFKRIYAKTMKLLDYMTGEFLV
                     EVTITDEERIRALNKEYRAKDAATDVLSFAFNDIKPGHKFKEPFVHLGSIIICAPVAQ
                     RQALELGHDARREFKFLFTHGLLHLLGYDHTNEKEEQVMITLQDQIIGKRGGKK"
     gene            982..1380
                     /gene="cdd"
                     /locus_tag="PLQ55_04840"
     CDS             982..1380
                     /gene="cdd"
                     /locus_tag="PLQ55_04840"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPK29291.1"
                     /translation="MTKEELIKKAREARALAYVPYSKFKVGAALLTKSGKVYVGANVE
                     NASYGLCNCAERNALFHAMMNGEKKEDFVALAVTGKTAGPISPCGACRQVMSELLPKD
                     APIYLTNLEDAFLETTIAELLPFAFDDSDL"
     gene            1377..>1570
                     /locus_tag="PLQ55_04845"
     CDS             1377..>1570
                     /locus_tag="PLQ55_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPK29292.1"
                     /translation="MIKSGFVSIAGRPNAGKSTLLNTILGEKRAIVTPKPQTTNKNIQ
                     GVYNDLRGQIVFIDTPGIHKP"
BASE COUNT          518 a          256 c          293 g          493 t
ORIGIN      
        1 gcaattgatt gcacaagtac tgcgccttga ctaaaaccta aaagcgtgat gtctttcttt
       61 tctaaattat attgagtgat agcattaact ataaaatttt gtacactttc cactgcgctt
      121 tgatattgtt caagattagg gttttctaag tcttgcatgt cccaaaatgc gtatccacca
      181 aacatgaaac tgagcggtcc gcgcacacta aaaatatgat gggtgctttc taaacttnnn
      241 nnnnnnnttt tcatcgcttc gatagccgtg aaataaaaaa accgctggta atttttcatc
      301 tgtaactttt ggtgcggaat atataaaggt aaagtcagat ttcattgcta tttcctttct
      361 tttaatgctt tgtttttaag aatgtaacaa ataagaattt tatatacatt atcttaacat
      421 tgtactatgt atatttcaaa taccacttta gtggtttact ttattactcg attaaagcac
      481 tgcgttaatt taatcaatct atggtaaaat taatatgatg cttaaactta ctgttgaatt
      541 ttatgatgat tatgcaccga cttataaaaa ctatcgggct atttttaaac gaatttatgc
      601 aaaaacaatg aaattacttg attatatgac aggtgaattc cttgttgaag ttacaattac
      661 tgatgaagaa aggattaggg cattaaataa agaatatcgc gctaaagatg cagcaactga
      721 tgtattatca tttgccttta atgatattaa acctggtcat aagtttaaag aaccctttgt
      781 acatttaggt tccattatta tttgtgcccc agtagcgcaa cgtcaagcgc ttgaattagg
      841 acacgatgcc cggcgtgaat ttaaattttt atttacgcac ggacttttac atttattagg
      901 gtatgatcac actaatgaaa aagaagagca ggtgatgatc actttgcaag accaaattat
      961 tggtaaaaga ggagggaaaa agtgacaaaa gaagagttaa ttaaaaaagc acgagaagca
     1021 cgtgccttag cctatgttcc ttattctaaa tttaaagtag gcgcagcgct attaacaaaa
     1081 agcgggaaag tatatgtcgg cgcgaatgtt gaaaatgcta gttacggatt atgtaattgt
     1141 gcggagcgaa acgctttatt tcacgcaatg atgaatggcg aaaagaaaga agactttgtc
     1201 gcactagcgg tcactggtaa aacagcggga ccaattagcc cgtgtggagc gtgccggcaa
     1261 gtgatgagtg aacttttacc aaaagacgct ccgatttact taacaaattt agaagatgca
     1321 tttttagaaa caacaattgc ggaactttta ccattcgcct ttgatgatag tgacttatga
     1381 ttaaaagtgg ctttgtttcc attgctggac gacctaatgc cggtaaatcg acacttttaa
     1441 acacgatttt aggtgaaaaa agagcgattg ttaccccaaa accgcaaaca actaataaaa
     1501 atattcaagg tgtgtataac gacttacgtg gtcaaattgt ttttattgat acaccgggta
     1561 ttcataaacc
//
LOCUS       DAOPCD010000219         1566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_63484_length_1566_cov_2.293845, whole genome shotgun sequence.
ACCESSION   DAOPCD010000219 DAOPCD010000000
VERSION     DAOPCD010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63484_length_1566_cov_2.293845"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PLQ55_04850"
     CDS             <1..651
                     /locus_tag="PLQ55_04850"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HPK29293.1"
                     /translation="TNFSVDLIYGLPGQTLSDLENDLMNIIKLGAPHISTYSLTIEKG
                     TMAALKKWEVPSDDTLREMYDFILKTLRNNNYIRYEVSNFALKGYESIHNKLYWENRN
                     YYGIGLGASGYIDGKRYLISGGLTKYLKGESIINYDEITSEEYVEEYLMLNLRLAQGF
                     SLQDFKTRVGFDFLEKYGTKVKSLIERGLLNVTDDKVYATDEGIMLLDQVVLTLIM"
     gene            731..1015
                     /locus_tag="PLQ55_04855"
     CDS             731..1015
                     /locus_tag="PLQ55_04855"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPK29294.1"
                     /translation="MDKVVDIERLRDFFKVLGDDTRMRIILLLEESAKNVTEIANALN
                     MTQSAISHQLRTLYDHRVVTYSKAGQERIYEILDPHIYEIIKQARDHIRE"
     gene            1020..>1566
                     /locus_tag="PLQ55_04860"
     CDS             1020..>1566
                     /locus_tag="PLQ55_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPK29295.1"
                     /translation="MIQDNKQSKFQKIFHELGLYAYFIGLLLFLFALLAFIPGVSFLS
                     DKAVMILNLIALILAGYKVISEGLVETVKESIKSKRFRPNIHILMALGAIGAIIIGDY
                     GDAALLILIFAGAYFLEEYAEAKSAKEITNLLKITPTTARLLLQDGTQQEVSVESLNV
                     GDIVLVFNGEQIPTDGSIVSGS"
BASE COUNT          542 a          228 c          313 g          483 t
ORIGIN      
        1 acaaacttta gtgttgattt aatttacgga ttacctggcc aaactcttag cgacttagaa
       61 aatgacttaa tgaacatcat aaagctagga gcaccacata tttctaccta tagcttaacg
      121 attgaaaaag gcacgatggc tgcacttaaa aagtgggaag ttccaagtga cgacacttta
      181 cgcgagatgt atgactttat tttaaaaaca ttaagaaata ataattatat acgctatgaa
      241 gttagtaatt ttgcgttaaa gggatatgaa agtatccaca ataaactata ttgggaaaac
      301 cgtaattatt acgggattgg ccttggcgct agtgggtata ttgatggtaa acgctactta
      361 ataagtggcg gtttaactaa gtatttaaaa ggcgaaagta tcataaatta cgacgaaata
      421 acaagcgaag aatatgttga agagtactta atgcttaact tacgactcgc acaaggtttt
      481 agtttacaag actttaaaac tcgtgtcggc tttgattttt tagaaaagta tggcactaag
      541 gtaaaaagcc tcatagaaag aggactttta aatgtcactg atgacaaagt atatgcgaca
      601 gatgaaggga ttatgctcct tgatcaagtc gttttaacat taataatgta aatatcttta
      661 catatattag atatattttt tataatgtat atgaacactt gctcaaatat tcaagtgtta
      721 ggagaaaatt atggataaag tcgtggatat tgaaagatta cgagacttct ttaaagtctt
      781 aggagatgat acgcgtatgc gtattatttt actcttagaa gaaagcgcaa agaatgtcac
      841 tgaaatcgct aatgcattaa atatgacgca aagtgcgatt agtcatcaat tacggacact
      901 ttatgaccat cgcgtagtca cgtattcaaa agctggccaa gagcggattt atgaaattct
      961 tgacccgcat atttatgaaa ttattaaaca agcacgtgac catattaggg agtaaaaata
     1021 tgatacaaga taataaacaa agtaaatttc aaaaaatatt tcacgagctt ggtctctatg
     1081 cttattttat cgggctattg ctctttttat ttgcactttt agcatttatc cccggtgtga
     1141 gttttttaag tgataaagcg gtaatgatat taaacttaat agcgcttatt cttgcgggtt
     1201 acaaagttat tagtgaagga ctagtcgaaa cggttaaaga gagtattaaa agtaaaagat
     1261 ttcgtccaaa tattcatatc ttaatggcac taggggcgat tggggcgatt attattggtg
     1321 attatggcga cgctgcttta cttatattaa tattcgcggg cgcgtatttt cttgaagaat
     1381 atgctgaagc aaaaagtgcc aaagaaatta cgaatctttt aaagataaca ccgacaaccg
     1441 ctaggctttt acttcaagac ggaacacaac aagaagtaag tgttgagtca ctaaacgttg
     1501 gtgatatagt acttgtgttc aatggtgagc aaatcccgac agatggctcg atagttagtg
     1561 gtagtg
//
LOCUS       DAOPCD010000220         1547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_64620_length_1547_cov_3.247319, whole genome shotgun sequence.
ACCESSION   DAOPCD010000220 DAOPCD010000000
VERSION     DAOPCD010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64620_length_1547_cov_3.247319"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..>1547)
                     /locus_tag="PLQ55_04865"
     CDS             complement(184..>1547)
                     /locus_tag="PLQ55_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPK29296.1"
                     /translation="HLQLMPVYDFKTVDETRKWQSYNWGYDPQQYFVLEGSYARDPLH
                     PYNRISDFKLMVSRYHEVGIRIIMDVVFNHVYDHETSVFEKIVPNYYFRKTSEGVISN
                     GSGCGNDLATERPMVRRLVVDACRYYAEKLGVDGFRFDLMGLIDIKTMEEVTKAVRQV
                     KPDFLILGEGWEMMTPHPKEKLAHHGNASKTPEIGFFNDAFREIVKGGSFADNLHERG
                     YMLGATNYRLGFYYAYAGSVLNTTFTPRYLNAGQSINYVECHDNGVLVDKMTVSNASE
                     CKETHLARLLSMNTIVMLSFGVPFFHRGQEIGVSKYGDLNSYKSSDKVNQFPYTLALK
                     RKEMVEYFKALTALRKDCKFLHETDPKVIKNIIEFKDLPGGASQIVYNPDMDLSPYSH
                     FNVYVNPTSEPIFISLEKPLKTILSSGGYIAARSKEYFERTMVAPYSVIVLGLRKGDS
                     GPK"
BASE COUNT          464 a          332 c          267 g          484 t
ORIGIN      
        1 aaggctgaag gtgtattcga ccaatgtttg actttaaagt agtcatactt tgctctttct
       61 tcatcatcct cataatctaa ccctggcact ttctcgacaa gtaaatagcg ataatcattt
      121 tcgtttttac ctagggtacc aacgataaat acggatgtta acataatcct tcctcctatt
      181 agtttatttt gggccagaat ctcctttccg tagtcctagc acaattacac tatagggggc
      241 aaccatcgtt ctttcgaaat attctttcga gcgagcggcg atatatccgc cactacttaa
      301 aattgtttta agtggttttt ctaaactaat aaagattggt tcactagtgg gattaacata
      361 cacattaaag tgactatacg ggcttaaatc catatcggga ttgtacacga tttgacttgc
      421 gccacctggt aaatctttaa attcaattat attcttaatg actttaggat cagtttcatg
      481 taaaaattta caatctttgc gtagcgctgt taaagcttta aaatattcaa ccatttcttt
      541 acgtttcaag gctaaagtat acgggaattg gttaacttta tcgctgctct tatatgaatt
      601 taagtcacca tattttgaca caccaatttc ttgtccgcga tggaagaaag ggacaccaaa
      661 agagagcatg acaatcgtat tcatactaag gagtctcgct aaatgagttt ccttacattc
      721 actagcgtta ctaaccgtca ttttatcaac taaaacaccg ttatcatgac attcaacata
      781 gttgatactt tgtccagcgt taagatagcg gggcgtaaat gttgtattaa gcacactacc
      841 agcgtaagca taataaaagc ctaagcgata atttgtggcg cctaacatat aaccgcgttc
      901 atgaaggtta tcggcaaaac taccgccttt aacaatttcg cggaacgcat cattaaagaa
      961 gccaatctct ggtgtcttac tagcgttacc gtgatgcgct aatttttctt taggatgcgg
     1021 ggtcatcatc tcccagcctt cacctaaaat taagaaatca ggttttactt ggcgcacggc
     1081 ttttgtgact tcttccattg ttttaatatc aattaaaccc attaagtcaa aacggaaccc
     1141 gtcaacccct aatttttccg cgtaatagcg acaggcatca acgacaaggc gccgtaccat
     1201 cgggcgttcc gttgctaaat cattaccgca accactgcca ttactaatca caccttcact
     1261 tgttttacgg aagtagtaat ttggtacaat cttttcaaaa acactcgttt catggtcata
     1321 aacatgatta aaaacaacgt ccataataat acggattccc acttcgtgat aacgactgac
     1381 cattagttta aaatcactaa tacggttata gggatgaagc ggatcacggg cataacttcc
     1441 ttctaacaca aaatattgct gcgggtcgta gccccagtta tagctttgcc atttacgcgt
     1501 ttcatcaacg gttttaaagt cataaacagg cattaattgt aagtgtg
//
LOCUS       DAOPCD010000221         1541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_65009_length_1541_cov_2.288022, whole genome shotgun sequence.
ACCESSION   DAOPCD010000221 DAOPCD010000000
VERSION     DAOPCD010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65009_length_1541_cov_2.288022"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1102
                     /gene="tgt"
                     /locus_tag="PLQ55_04870"
     CDS             2..1102
                     /gene="tgt"
                     /locus_tag="PLQ55_04870"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006526259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPK29297.1"
                     /translation="MHECTQSGARFGFLHTPHGKVEIPMFMPVGTLANVKTITPEEIV
                     ALGSGVILANTYHLSLRPGTDVIKKAGGIHSFMNYHGPILTDSGGFQVFSLADNRKIT
                     EEGVTFRSHLDGKELFFSPEEAIRLQEEIGADIIMSFDECIHYPADYDYVKESTERTL
                     RWAKRGKEAHKRKDQALFGIVQGGEYPDLRKYSAEETVKIGFHGYAIGGTSIGEPKAI
                     FIKMIESAVPYLPKDKPRYLMGIGSIDMILQGIARGVDMFDCVLPTRIARHGSLFTRT
                     GRLNIRRAEFKEDFAPIEEGCDCFTCQKFTRAYLRHLYISEEALAQRLLSIHNLRFLI
                     KLMEDARVAIKENRFLDFKNEVIKTFGDKRGF"
     gene            1102..>1541
                     /locus_tag="PLQ55_04875"
     CDS             1102..>1541
                     /locus_tag="PLQ55_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HPK29298.1"
                     /translation="MDVNLFDFNLPKELIAQYPTISRQNARLLVFNKEKNELKDEHFY
                     DIIKYFNTGDVLVLNNTKVIPARLRGIKVPTGAKIEVILLKALKNDEYEVLIGNAKAI
                     KVGTIVSFGGGKLLMECLTKYKEGIHIVRFKYEGVFLEILEKIGT"
BASE COUNT          497 a          250 c          320 g          474 t
ORIGIN      
        1 actgcatgaa tgtacccaaa gcggggcacg atttggtttt ttacatacac cacatggtaa
       61 agttgaaatt ccgatgttta tgccagtggg aacgctcgct aatgtaaaaa caataacccc
      121 cgaagaaata gtcgctttag gtagcggtgt tattttagca aacacgtatc acctttcttt
      181 aagaccaggt actgatgtaa ttaaaaaggc tggtggcatc cactctttta tgaattacca
      241 tggtcctata ttgactgatt cgggcggttt tcaagtcttc tcgttagcgg ataatcgcaa
      301 aatcactgaa gaaggcgtta cttttagatc gcatttagat ggtaaagaac ttttcttttc
      361 gcctgaagaa gcaattagac ttcaagaaga aattggcgcg gacattatta tgagttttga
      421 tgaatgtatt cattatcccg ctgattatga ttatgtaaaa gaaagcacgg aaagaacttt
      481 acgttgggca aagcggggaa aagaagcaca taaacgtaaa gatcaagcgt tgtttgggat
      541 tgttcaaggg ggagaatatc ctgatttacg caaatatagc gccgaagaaa cagttaaaat
      601 tggatttcat gggtatgcta ttggcggcac aagtattggt gaacccaaag ccatctttat
      661 taaaatgatt gagagtgccg ttccctattt accaaaagat aaaccgcgtt atttgatggg
      721 tattggttcg attgatatga ttctccaagg aatcgcgcgc ggtgttgata tgtttgattg
      781 tgtcttacca acaaggattg cccgtcacgg atcgctattt acgagaacag gccgccttaa
      841 tatccgccgt gctgaattta aagaagattt tgctcctatt gaagaaggat gcgactgctt
      901 tacttgccaa aaatttacac gcgcttattt acggcacctc tatattagtg aagaagcact
      961 tgcacaacgc ctactctcaa ttcataactt acgattttta attaaattaa tggaagacgc
     1021 gcgagttgct attaaagaaa atcgtttttt agattttaaa aatgaggtta ttaaaacgtt
     1081 cggcgataag cgggggttct aatggatgtt aacttatttg actttaattt acccaaagaa
     1141 ctaattgcgc aatatcccac tatttcgcgg caaaatgccc gtcttttagt atttaataaa
     1201 gaaaaaaacg aattaaaaga tgaacatttt tacgatatta ttaagtattt taatactggt
     1261 gatgttttag ttttaaacaa tacaaaagtt atacccgcaa gactgcgcgg tattaaagtt
     1321 cctacaggtg caaagattga agttatactg ttaaaagctc ttaaaaatga tgagtatgag
     1381 gtactaattg gaaatgctaa agcaattaaa gtcggtacca ttgtttcttt tggcggcggt
     1441 aaacttttaa tggaatgcct tactaaatat aaagaaggaa ttcatatagt aaggtttaaa
     1501 tatgaaggag tttttcttga aattttagaa aaaatcggga c
//
LOCUS       DAOPCD010000222         1535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_65390_length_1535_cov_2.256757, whole genome shotgun sequence.
ACCESSION   DAOPCD010000222 DAOPCD010000000
VERSION     DAOPCD010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65390_length_1535_cov_2.256757"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1481)
                     /locus_tag="PLQ55_04880"
     CDS             complement(132..1481)
                     /locus_tag="PLQ55_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29299.1"
                     /translation="MKNKKAIALILINVLALGVMGVLHSLRKNGLLGGVDANPLSPAY
                     TRTLTALDGPNGTMPMRGTIVAKDDTYYYSSASSQNDDSFLVLSASDAKNPGVFMNTT
                     VINGIGEVRVTFSGSGNLYAIVSETLFERFTPSASDALTSGVKKEFATVGGYLLILND
                     SLSPITIESLEIYFHCAHDIDSKFYFEQGVNLYTGARSLGSNIVIGHDELSFKTNPTA
                     TTNNYAGETIVPPAERPNAWYRWNGVSMRNYGGTLETPNYTTTRFGEFYSSSFKISTT
                     AFVDPSVFYDTDEWFCVAPWVSLSASGKPRTERHYLQSYIGNDNYDPLTTVNELYKPY
                     FSGRFYTNYGYTESAWEFQNPDTVTVVDDATMTLREAYEAINLPFFNVTYEISGNTYK
                     VFINGFYVYTDTILETYTDETFALETFELQAVNYGNEDGSPKAGYNVTYSNPVIKTI"
BASE COUNT          479 a          298 c          257 g          501 t
ORIGIN      
        1 ttattgtaac agttttaaat taaagagggt cgttactttt atttagaaat gtgaatatat
       61 ttaacacatt atatatttta aaaaagcagg cctacttatt aagcctgctt gccatgtatt
      121 tttaaaatat tttaaattgt tttaatgaca gggttagaat aagtaacatt gtaaccagct
      181 tttggtgaac catcttcatt cccataattg acagcttgta attcaaaagt ctcaagcgca
      241 aaagtttcat ccgtgtaagt ttcaagaatc gtgtcagtat atacataaaa tccattaata
      301 aacactttat atgtattgcc acttatttca taagtaacat tgaaaaacgg taaattaatt
      361 gcttcatatg cttctcttaa tgtcattgtc gcatcatcaa caacagtaac agtatctggg
      421 ttttggaact cccaggcact ttcggtataa ccataattag tgtagaaacg accggaaaaa
      481 tacggcttat aaagctcatt aaccgtcgtt agtgggtcat aattatcgtt accgatatat
      541 gactgcaagt aatggcgttc tgttcgcggt tttcccgatg cacttaaact tacccatggc
      601 gccacacaaa accattcatc agtgtcataa aacacacttg gatcaacaaa agctgtcgta
      661 cttatcttaa atgaagaact ataaaactct ccaaacctag tggtcgtata atttggtgtt
      721 tctaatgtgc ccccataatt acgcatactt acaccattcc atcgatacca tgcattaggg
      781 cgctcggccg gagggactat tgtttccccg gcataattat ttgtcgtggc ggtcggatta
      841 gttttaaagg agagttcgtc atgaccgata actatattac ttcctaatga ccgcgcacca
      901 gtgtagagat taacgccttg ttcaaagtaa aatttactat caatgtcatg cgcacaatgg
      961 aaatatattt ctaaactttc aattgtaatt gggcttagtg aatcatttaa aattagtaag
     1021 taacccccaa ctgtcgcaaa ttctttttta acgccacttg ttagagcatc acttgcacta
     1081 ggggtaaaac gctcaaataa tgtttcactt actattgcat aaagatttcc gctgcctgaa
     1141 aatgttactc ttacttcccc aattccgtta atgacagttg tgttcataaa tacaccaggg
     1201 tttttagcat cacttgcgga taaaacaagg aaactatcgt cattttggga acttgcactt
     1261 gaataataat aagtatcatc tttagccaca attgtcccgc gcattggcat agttccgttt
     1321 ggtccatcaa gcgcggttaa agttcgagta tacgccggtg aaagcggatt agcgtcgact
     1381 ccacctaata atccattttt gcgtaatgaa tgtaagaccc ccattacacc aagggctaaa
     1441 acgttaatga gaattaaggc tattgctttc ttgtttttca taatatttat tccttttttc
     1501 ttaagtatat aataacttgc aataaatgtc aaagt
//
LOCUS       DAOPCD010000223         1528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_65828_length_1528_cov_3.219280, whole genome shotgun sequence.
ACCESSION   DAOPCD010000223 DAOPCD010000000
VERSION     DAOPCD010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65828_length_1528_cov_3.219280"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1528)
                     /locus_tag="PLQ55_04885"
     CDS             complement(<1..>1528)
                     /locus_tag="PLQ55_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29300.1"
                     /translation="KYKFFDTNRQYQDALKRKLGNLKDKYFSLLKKAVSFSPITDITT
                     FITEYVTEAQGNIDIAPMQENILQYKKLEDEAALMRFKVERLNEIAKNYEVYKGHKEQ
                     LTLFEYVLEKATLQNDLNRLATYDNQYLGYQKRLTDIESELADLDVDLAELNKRRVKL
                     IQDSSSNDTYRITDEINEHKKVAEEKIKSLKFLNMQVRSDLENYVLTFDRLANEVLEK
                     VSAMDEKRLKKDGLYAREELLESAETIIKENHKITSVLNKDINELSETMLNDWRSAQA
                     KFKQHLGAFNLSLGRLINDYQVNEAILKQQELDLRGGGKPYEYSLVQIRNELQNKLSA
                     KYGHKVEVSIFSDLIDIRDAKWTNAIEGYLGGQRFHLFVEGEYFLDAYEFLKGLLQQY
                     NFYGTNLVDVDAIIERRFSAMPNSVAEEILTDHIGARAYADFLLGRLQKCNTIQEARN
                     SGNGITPNTDLYRNFTFGRINPRLYQQKYIGRRVEEADVNAKKAELAAHAETLRSYQE
                     L"
BASE COUNT          449 a          283 c          236 g          560 t
ORIGIN      
        1 taactcttga taagaccgta atgtttcagc gtgagcagca agttcagctt tcttcgcgtt
       61 aacatccgcc tcttctacgc ggcgtccaat atatttttgt tggtataagc gcggattaat
      121 acgcccaaaa gtaaagttcc gataaagatc agtattaggc gtaataccat taccactatt
      181 gcgtgcttct tgaattgtat tacatttttg taaacgacca agcaagaaat cagcgtatgc
      241 tcttgcccca atgtggtcag ttaatatttc ttcagcaaca gaatttggca ttgcactaaa
      301 gcggcgctca attatcgcgt caacgtccac taaatttgtg ccatagaaat tatattgttg
      361 taaaagtcct tttaaaaatt cataagcatc aaggaaatat tcaccttcga caaaaagatg
      421 gaagcgttgg ccacctaagt aaccttcaat cgcatttgtc cacttcgcat cgcgaatatc
      481 aattaagtca ctaaaaatac ttacttcgac tttatgacca tattttgccg aaagtttatt
      541 ttgtaattca ttgcgaattt gcacaagact atattcatat ggtttaccac caccgcgtaa
      601 atctagttct tgttgtttta aaattgcttc atttacttga tagtcattaa ttaaacgacc
      661 aagacttaag ttaaaagccc caagatgttg cttaaatttc gcttgagcac tgcgccaatc
      721 attaagcatt gtttcactaa gttcattaat gtctttattt aaaacactag tgattttatg
      781 attttcttta ataattgttt cggcgctttc aagtagttct tcgcgcgcat ataatccgtc
      841 tttctttagc cgtttttcat ccatcgcact aactttttct aaaacttcat tagcgagtct
      901 atcaaatgtt aggacataat tttctaagtc actacggact tgcatattta agaatttaag
      961 acttttaatt ttttcttctg cgactttttt atgttcatta atttcatcag tgatgcgata
     1021 agtatcatta cttgaactat cttgaattaa tttaacacgg cgtttattaa gttcagcaag
     1081 gtcaacatct aaatcagcaa gttcactttc aatatctgtt aagcgttttt gataacctag
     1141 atattgatta tcataagttg ctagtcgatt taagtcattt tgtagtgttg ctttttctaa
     1201 gacatattca aaaagtgtta attgttcttt atgacctttg tatacttcat agtttttagc
     1261 tatttcattt aagcgttcaa ctttaaaacg catcagcgct gcttcgtctt caagcttttt
     1321 atattgcaaa atattttctt gcattggtgc aatatcaata ttgccttgtg cttcagtgac
     1381 atattcagta ataaaagtcg taatatccgt aatgggtgag aaactaacag ctttctttaa
     1441 gagactaaaa tacttatctt ttaaattacc aagtttccgt ttaagggcat cctgatattg
     1501 acggttcgtg tcaaagaatt tgtattta
//
LOCUS       DAOPCD010000224         1527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_65885_length_1527_cov_3.740489, whole genome shotgun sequence.
ACCESSION   DAOPCD010000224 DAOPCD010000000
VERSION     DAOPCD010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65885_length_1527_cov_3.740489"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1374
                     /locus_tag="PLQ55_04890"
     CDS             <1..1374
                     /locus_tag="PLQ55_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK29301.1"
                     /translation="ITMLVIHLWDAVNDPIIGMLIENSRFKLGKYKPWILIGGVLNGL
                     LLMVLFSFRPFGGWGYVIFFIVTYLLWEISFTLNDIGYWGMVPSLTRDPKQRNQITML
                     MAIAINIGAFTVGGLVPSLYPGRAIQVFRTLSIIIGSVLIFSQLLLVFLCKERDRSEV
                     DSEEIIKVKDIFKVVFQNKPLLVMLGAIVLHYLGGSMFNNLGQNYMHFTFGYAEGGQA
                     FFYFLVGYAVGSIAANFIFGFLVKKYTRAQILRGAFTISAIGNSLLFVIGSTHAFGWL
                     QIPNSIYQWILPVLALIIFTPNGVFYLTLLVQITNTVEYNEWKFGERKEGVVFAARPF
                     AAKISNAITVIVVFLTLTVGGVYDITNKISDYEVLGGLPEGDARRLITEEVQDLAQAA
                     IDGANLGRARFVLILGMALLPLICQTLAYLLVSRKYPIDEKMYDQMVKEIDERRETAA
                     EEQVVEA"
BASE COUNT          422 a          262 c          323 g          520 t
ORIGIN      
        1 attacgatgt tggtcattca cttgtgggat gcggttaatg acccaattat tgggatgtta
       61 attgaaaatt cacgctttaa actcggtaaa tataaaccgt ggatcttaat tggtggggta
      121 ctaaacggtt tgttactcat ggtgctcttt agttttcgac catttggtgg ttggggctat
      181 gttattttct ttattgttac ttatctctta tgggaaatta gctttacgct taatgatatt
      241 gggtattggg ggatggttcc tagtcttacc cgtgacccaa agcaacgtaa tcaaattacg
      301 atgctgatgg ctattgcgat taatattggt gcttttaccg ttggcggact cgttccaagt
      361 ttatacccag gacgcgcgat tcaagtattt agaacactta gtattattat tggtagtgta
      421 ttaattttct cccaattatt acttgtgttc ttatgtaaag agcgagatcg tagtgaagtt
      481 gatagcgaag aaattattaa agttaaagac atctttaaag ttgtcttcca aaataaaccg
      541 cttttagtaa tgcttggggc aattgtcctt cattacttag gtggttcaat gtttaataac
      601 cttggtcaaa actatatgca ctttactttt ggctacgctg aagggggcca agctttcttt
      661 tatttcttag tggggtatgc tgttgggtca attgccgcga actttatttt tggcttcctt
      721 gtgaaaaaat atacaagagc acaaatctta cgcggcgcct tcactattag cgcaattggt
      781 aactcactgc tctttgtgat tggaagtaca catgcttttg gttggttaca aattccaaat
      841 agcatctacc aatggatttt acctgtttta gcgctcatta tttttacgcc aaacggtgtg
      901 ttttatctta cactgctcgt gcaaattaca aatacggttg aatataatga atggaaattt
      961 ggcgaacgga aagaaggtgt tgtctttgcc gcgcgtcctt ttgccgcgaa gattagtaac
     1021 gcaatcaccg ttattgttgt gtttttaaca ctcactgtcg gcggtgttta cgatattact
     1081 aataaaatta gtgattacga agtcttaggc gggttaccag aaggagacgc acggcgactt
     1141 atcactgaag aagtgcaaga cctcgcccaa gccgcaattg acggcgcaaa tttaggcaga
     1201 gcacgctttg tgttaatctt agggatggca cttttaccac ttatttgtca aacacttgcc
     1261 tatctccttg tcagtcgtaa atacccaatt gatgaaaaga tgtacgacca aatggtcaaa
     1321 gaaatcgacg aacggcgtga aaccgcagcg gaagaacaag tcgttgaagc ataagcgtta
     1381 attagaatta ccgaaaagtc caagttatca agacttgggc ttttttgtta aaaattcaat
     1441 ctttcaccat aaattgttag ttattttgta cttttttgtt gtgaggactt attttaagtt
     1501 attaaataaa tgagtatatt atagata
//
LOCUS       DAOPCD010000225         1525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_66020_length_1525_cov_2.361224, whole genome shotgun sequence.
ACCESSION   DAOPCD010000225 DAOPCD010000000
VERSION     DAOPCD010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66020_length_1525_cov_2.361224"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLQ55_04895"
     CDS             <1..429
                     /locus_tag="PLQ55_04895"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HPK29302.1"
                     /translation="RQITELQATLLFIKTYYPYERFIVETSYPFENGDETSIVNKDSF
                     PPKRGFLAYTPLGQERVLETILDAAVEEQITGLCYFEPGWINVNGLTVATIEGIKYLK
                     EKNIKAGSTLFNEALFSRSGRPNPALSVKNNMDAFKKSDN"
     gene            567..1463
                     /locus_tag="PLQ55_04900"
     CDS             567..1463
                     /locus_tag="PLQ55_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK29303.1"
                     /translation="MDIILQTNNLSKRFKNKLALKNVNMTIYRGDIYGFVGENGAGKS
                     TLIRLVTNILRPSTGSYSLNLPKRLGVVGAIVESPAFHPGLNALQNLRYQSDLLGYKK
                     TDEELLVLLDKVGLEEQRNSKKVVKNFSLGMKQRLSIAVTLLSNPEFILLDEPMNGLD
                     PAGIKEMRDLILELNRKGTTFLISSHILTELDKVATRYGFISHGELVEEISVRDLHKK
                     AKSYTEVKLGSEVNAKVRELLSGLNFKEASATTLHFATVAEAQNALKLLVANDISVTS
                     FEEIHETIEDYYLQKINKGVGA"
BASE COUNT          525 a          222 c          312 g          466 t
ORIGIN      
        1 cgccaaatta ctgaattaca agcgacactt ttatttatta aaacttatta tccttatgaa
       61 cgctttatcg ttgaaactag ttatcctttt gaaaatggtg atgagacaag tattgtaaat
      121 aaagatagtt tcccaccgaa aagaggtttc ttagcctaca caccgttagg acaagagcgt
      181 gtccttgaaa caattttaga tgcagcggtt gaggagcaaa ttacagggtt atgttatttt
      241 gaaccaggat ggattaatgt taatggttta actgtggcaa caatagaagg tattaaatac
      301 ttaaaagaaa aaaatatcaa ggccggcagt acattattta atgaagcttt gttttcaaga
      361 agcggccgac ctaatccagc acttagcgtt aaaaataata tggatgcttt taaaaagagt
      421 gataattaaa agggagagtc ctcgcaagta gcgagggctt ttcatttgta aagaaaagtt
      481 tactaacacg cttacgagtt tttcgtttag tgttaagtag tgtataatta caaagtaatt
      541 aaaagtgaaa gtagagaagt gcatttatgg atattatctt acaaacgaat aatctctcca
      601 aacggtttaa aaataaacta gcgctaaaaa atgtaaatat gacgatatat cgcggtgata
      661 tttacgggtt tgttggtgaa aacggcgctg gtaaatcaac actgattcgc ctagtgacga
      721 atattttacg gccttcgact gggagttatt cattaaactt accaaaaaga cttggcgtag
      781 tgggagcgat tgtcgaaagt cctgctttcc atcccggctt aaacgcttta caaaatttac
      841 gctatcaaag tgatttatta ggttataaaa agactgatga agagttatta gtgttactcg
      901 ataaagttgg gttagaagaa caacgcaata gtaaaaaagt cgttaaaaac tttagtttag
      961 ggatgaaaca acggttaagt atcgccgtga cccttttaag taatcctgaa tttattttgc
     1021 ttgatgaacc aatgaacggg cttgaccccg cgggcattaa agagatgcgt gacttaattt
     1081 tagaattaaa tcgaaaaggt acgacatttt taatttctag tcacatttta actgagttag
     1141 ataaagtagc gacgcgttac gggtttatta gtcacggtga attagtcgaa gaaatttccg
     1201 tgcgtgactt gcataaaaaa gctaagagtt ataccgaagt taaattaggt agtgaggtaa
     1261 acgctaaagt gcgtgaatta cttagcggtt taaattttaa agaagctagc gcgacaactt
     1321 tacactttgc gactgttgca gaagcacaaa acgctttgaa acttttagtc gctaacgata
     1381 ttagtgttac ttcttttgaa gaaatccatg aaacaattga agattattac ttacaaaaaa
     1441 ttaataaggg agttggtgcc taattatgaa aaaattatta attgctgatt tacggcggat
     1501 cttcaaaggt aaaggtattt atgtc
//
LOCUS       DAOPCD010000226         1511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_66913_length_1511_cov_3.269918, whole genome shotgun sequence.
ACCESSION   DAOPCD010000226 DAOPCD010000000
VERSION     DAOPCD010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66913_length_1511_cov_3.269918"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /gene="pheS"
                     /locus_tag="PLQ55_04905"
     CDS             <1..712
                     /gene="pheS"
                     /locus_tag="PLQ55_04905"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010288230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPK29304.1"
                     /translation="HPFHLVKDEICDIFIGMGYIIAEGDEVEEDLFNFTLLNMPENHP
                     ARDMQDTFYINEKLLLRTHTSPVQARVMQKNKNQPLKMISPGKVFRRDDDDNTHSHQF
                     GQVEGLVIGEGISFSHLKATLDLFAKKMFGPKREIRLRSSYFPFTEPSVEVDVSCHVC
                     NGKGCSTCKGTGWIEILGAGMVNPNVLTMNGYDTEKFTGFAFGVGIERVAMLRYGIDD
                     IRRFYQNDIRFLKEFPKR"
     gene            722..>1511
                     /locus_tag="PLQ55_04910"
     CDS             722..>1511
                     /locus_tag="PLQ55_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HPK29305.1"
                     /translation="MKISRNALERYIDLSTLSDKEIADGLTFAGIETEDYYKLASGTN
                     LVIGHVLTCIKVEGSDHLSYCTVDVGRLGTREIICGAPNVRSGQKVIVALEGAILPVG
                     EIKRVTIKGHNSEGMICSLSELGVPSELQSHDDLVGIHVLSEDAPVGNTQVLEYLGLD
                     DTIFDIRPLANRSDMLALYNVVRELGAIFDLHYNIPEIEVNPSFKTKLKLEVNTPLVP
                     IFALTEVRNVAIKPSPQWMATFLRKHGHRPVNNIVDIGNYVMLVT"
BASE COUNT          477 a          260 c          332 g          442 t
ORIGIN      
        1 acaccctttc cacctcgtta aagatgaaat ttgcgatatc tttatcggga tgggctacat
       61 tattgcggaa ggcgacgaag ttgaagaaga tctctttaac tttactcttt taaatatgcc
      121 ggaaaaccat ccagcgcgtg atatgcaaga taccttctat attaatgaaa agttattact
      181 tagaacacat acttcaccgg tacaagcacg cgtaatgcaa aaaaataaaa atcaaccact
      241 aaaaatgatt agtccgggga aagtattccg cagagatgat gatgataata cccattccca
      301 tcaatttgga caagttgaag gcttagtaat tggggaaggc atttctttta gtcacttaaa
      361 agcaacactt gacttatttg cgaaaaaaat gtttggtccg aaacgagaaa tacgcttacg
      421 ttcaagttat ttcccgttta ctgaacctag tgtggaagtt gatgttagtt gtcacgtatg
      481 taacggtaaa gggtgctcga cttgtaaagg cactggttgg attgaaatct taggagcggg
      541 aatggttaat cctaatgtgt taacgatgaa tggctacgat acggagaagt ttaccggatt
      601 tgcctttggt gtcgggatcg aacgcgtagc gatgctccgc tacgggattg atgacattcg
      661 ccgcttttat caaaatgaca tccgtttctt aaaggaattc ccaaaaaggt aggtaataat
      721 tatgaaaatt tcacgaaatg cattagaacg atacattgat ttaagtactt taagtgataa
      781 agaaattgcg gacggactca ctttcgcggg aattgaaacc gaagactatt ataaactcgc
      841 tagtggtacg aatttagtaa ttgggcatgt tttaacttgc attaaagttg aaggtagtga
      901 tcacctttct tactgcacgg ttgacgttgg tagattagga acacgcgaaa ttatatgcgg
      961 ggcgcctaat gtgcgaagcg gacaaaaagt tattgtcgcg cttgaaggag caattttacc
     1021 agtcggtgaa attaaacgcg tcacgattaa aggacataat agtgaaggga tgatttgttc
     1081 acttagcgaa ttaggagtgc ctagtgaatt acagtcgcac gacgatttag taggtatcca
     1141 tgttctaagt gaagacgcac cagttggaaa tacacaagtt ttagaatatt taggattaga
     1201 cgacactatt tttgatatta gaccactcgc taacaggagt gatatgcttg cgctatataa
     1261 tgttgtccgt gagcttggcg cgatttttga cttacattat aatattccgg aaattgaagt
     1321 taatccgtct tttaagacaa aacttaaact agaagttaac acaccgcttg tgccaatctt
     1381 tgctttaaca gaagtaagga atgtggcaat taaaccatcc cctcagtgga tggcaacttt
     1441 cttacgtaaa cacggacaca gacctgttaa taatattgtt gatattggaa actatgtgat
     1501 gcttgtcaca g
//
LOCUS       DAOPCD010000227         1494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_68042_length_1494_cov_2.850591, whole genome shotgun sequence.
ACCESSION   DAOPCD010000227 DAOPCD010000000
VERSION     DAOPCD010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68042_length_1494_cov_2.850591"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /gene="yihA"
                     /locus_tag="PLQ55_04915"
     CDS             complement(<1..392)
                     /gene="yihA"
                     /locus_tag="PLQ55_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPK29306.1"
                     /translation="MINFQNTLFVGSFTNKNEGAIEALPEIIFIGRSNVGKSSLINAI
                     INRKNLAYTSKKPGFTKLLNYYNVDDTFYLVDAPGYGFAKREKDLSASFATMMNNYFN
                     KNTNLSMIYLLIDSRRTLTADDEAFLDFV"
     gene            complement(400..>1494)
                     /locus_tag="PLQ55_04920"
     CDS             complement(400..>1494)
                     /locus_tag="PLQ55_04920"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HPK29307.1"
                     /translation="PIFVLDEVDKLGKSNRGDPSSALLEILDPEQNKGFIDHYIDEPY
                     DLSKVVFIATANYYWDIPRPLRDRMEMIELKGYTEEEKVQIAEKHLIPNGIESHGLKG
                     YDIKFTDEALRFIIGGYTWEAGVRMLNQKISAIFRKLSVEVLSDVESNFTITPEEVQK
                     HLGKPRNFFTKKEPEPIVGVVTGISVAGNIGDIMKIEVTTFKGKGVVNVTGNLEKMLK
                     ESGEIAASHVRSYAEKYGINPDVFLDTDINVHFPQASTKDGNSAGVAMTIGIISVLTD
                     RKINPNVAMTGEVTLTGRVLAIGGLYEKILGCIRAGIETVLFPEENVVDLDEIPQEVK
                     DKVKLVPIKNIDEAVAIALLPKKVKKKATA"
BASE COUNT          433 a          317 c          240 g          504 t
ORIGIN      
        1 acaaagtcta aaaaagcttc gtcatccgcg gttagtgttc gtctactatc aattagtaaa
       61 taaatcatgg ataaattagt gtttttatta aagtaattat tcatcatagt tgcaaaacta
      121 gcgcttaaat ctttttcacg ctttgcaaac ccgtagccag gagcgtcgac taaataaaat
      181 gtatcatcaa cattataata gtttaataat ttcgtaaatc caggtttttt ggaagtatac
      241 gctaagtttt tacgattaat aatcgcgtta attaaactac ttttaccaac attactacgc
      301 ccaatgaaaa taatctcggg caaagcttcg attgcccctt cgtttttgtt tgtaaaacta
      361 ccaacgaaca atgtattttg aaaattaatc atagtacttt taggcagtcg cttttttctt
      421 tacttttttc ggtaaaagcg caatggctac tgcttcatca atatttttaa tcggcacaag
      481 tttaacttta tctttcactt cttgcgggat ttcatcaagg tcaacaacat tttcttcagg
      541 gaataatact gtttcaatcc ccgctctaat acagcctaaa atcttttcat aaagcccgcc
      601 gatggcaagt actcggccag ttaaagttac ttcaccagtc atcgccacat ttggattaat
      661 cttacgatca gttaatacac taataatgcc aatcgtcatt gcgacacccg cactattgcc
      721 atctttcgta cttgcttgtg ggaagtgtac atttatatca gtgtcaagga agacatcagg
      781 attaatccca tacttttcag cataacttct aacatggctt gcagcaattt cccctgattc
      841 tttaagcatt ttttcaaggt tccctgttac attaacaaca cctttacctt taaatgttgt
      901 tacttcaatc ttcatgatgt caccaatatt accagcgaca ctgatgccag tgacaacacc
      961 gacgattggt tctggttctt ttttggtaaa gaagttacgc ggtttaccta aatgcttttg
     1021 cacttcttca ggggtaatcg taaagttact ttccacgtca cttagtactt caacacttag
     1081 cttacggaaa atggcgctga ttttttgatt aagcatccgt acaccagctt cccaagtata
     1141 gccaccgata ataaagcgta aggcttcatc agtaaactta atgtcatagc ccttaagccc
     1201 gtgactttca atcccgttag gtattaagtg cttttcagca atttgtactt tttcttcttc
     1261 ggtataccct tttaattcaa tcatttccat ccggtcacga agtggacgcg ggatatccca
     1321 gtaatagtta gcggtggcaa taaagacgac tttacttaag tcatatggtt catcaatata
     1381 gtgatcgata aaacctttat tttgttcagg gtcaagaatt tctaaaagtg cactgcttgg
     1441 gtcaccacga ttgctcttcc ctaatttatc gacttcgtca aggacgaaaa ttgg
//
LOCUS       DAOPCD010000228         1487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_68492_length_1487_cov_3.886872, whole genome shotgun sequence.
ACCESSION   DAOPCD010000228 DAOPCD010000000
VERSION     DAOPCD010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68492_length_1487_cov_3.886872"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..570
                     /gene="spx"
                     /locus_tag="PLQ55_04925"
     CDS             124..570
                     /gene="spx"
                     /locus_tag="PLQ55_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009488107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HPK29308.1"
                     /translation="MIKIYTSPSCSSCRKVKQWFDEQQIPYEEHNIFSASLSEAELKE
                     ILYKSENGTDDIISTRSKVIKEKNIDVNDLTMSELVQFIKDNPSVLKRPIMVDTNRIQ
                     VGYNSEEIMAFIPRERRAQMEAELMMGVRQQNEDTEICLDDDCPTK"
     gene            complement(586..1164)
                     /locus_tag="PLQ55_04930"
     CDS             complement(586..1164)
                     /locus_tag="PLQ55_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HPK29309.1"
                     /translation="MATSIEIEAKALLTKKDYNKVLKHFKLDVVDGYIQKNYYIDTEN
                     KDLRKLGLSLRVRRLNGYVISFKLPMAEGLLEKTQTLSREQFEDFETHGKFPEGDISD
                     FIESLYISPSDLKIIATLTTLRIEQEYEDLTLAIDENRYGELQDFELEMEATSMKKAQ
                     YLLKKVCEEVGIDYKENLVSKQKRAMDALSAV"
     gene            1220..>1487
                     /locus_tag="PLQ55_04935"
     CDS             1220..>1487
                     /locus_tag="PLQ55_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPK29310.1"
                     /translation="MALKINVIVREDEKSLSVSKSLCELALQKGFLIDEVNPDFVFSV
                     GGDGTFLKAVHRYLTKVKLIKFVGIHTGSLGFFADFRPEDINNLL"
BASE COUNT          483 a          242 c          248 g          514 t
ORIGIN      
        1 aaacattata atgaagacaa gaaggatgta aatttaacct tttcctttaa cgaattcatt
       61 tcaggtattg cttttacata gtaaataagt tatattaata cgcggaacaa ggaggtcctg
      121 aatatgatta aaatttatac atcaccaagt tgttcgtcat gtcgtaaggt taagcaatgg
      181 tttgatgagc aacaaatccc atatgaagaa cataacatct tctctgcttc acttagtgaa
      241 gccgaactta aagaaatctt atataagtcg gaaaacggca ctgatgatat tatcagcact
      301 cgtagtaaag ttattaaaga aaaaaatatt gatgttaatg acttaacaat gagtgaatta
      361 gtgcaattta ttaaagataa tccgtccgta ttaaaaagac cgattatggt tgatactaat
      421 cggattcaag ttggctacaa tagtgaagaa atcatggcat ttatcccacg tgaaagacgc
      481 gcccaaatgg aagcggaatt aatgatgggt gtccgtcaac aaaatgaaga caccgaaata
      541 tgtcttgatg acgattgtcc tactaaataa ggtttattta ttttattaaa ctgcagaaag
      601 agcatccatt gctctttttt gtttgcttac aaggttttct ttataatcaa tcccaacttc
      661 ttcacatact tttttgagca gatattgggc ttttttcatc gatgttgctt ccatctcaag
      721 ttcaaagtct tgtagttcac cataacgatt ttcatcgatc gctaaagtta ggtcttcata
      781 ttcttgctca atgcgtaatg ttgttaaagt agcaataatc tttaaatcac ttggactaat
      841 atataaactt tcgataaagt cactaatatc gccttcaggg aatttaccat gagtttcaaa
      901 atcttcaaat tgttcacggc ttaatgtttg cgttttttca agtaatcctt ccgccatcgg
      961 tagtttaaaa gaaataacgt atccatttaa gcggcgaaca cggagactta atcctagttt
     1021 gcgcaaatct ttattttcag tatcaatata ataatttttc tgaatataac catcaacaac
     1081 atctagctta aagtgtttta atactttgtt ataatctttc ttcgtcagta gtgcttttgc
     1141 ttcaatttca atactcgtgg ccatagtaat tacctctttc tatcaatatg atacaatata
     1201 aacgaagatt tataaagtta tggcccttaa aattaatgta attgtgcgtg aagatgaaaa
     1261 gagtcttagt gtaagtaaaa gtttatgtga acttgcacta caaaaaggct ttttgattga
     1321 tgaagttaat cctgactttg tctttagtgt tgggggtgac ggaacatttt taaaagcagt
     1381 ccatcgctat ctcactaaag ttaaacttat taagtttgtt ggcattcata ctggttcact
     1441 tggattcttt gctgatttcc gtccggaaga tattaacaat cttttag
//
LOCUS       DAOPCD010000229         1463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_70165_length_1463_cov_4.472301, whole genome shotgun sequence.
ACCESSION   DAOPCD010000229 DAOPCD010000000
VERSION     DAOPCD010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70165_length_1463_cov_4.472301"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..>1463)
                     /locus_tag="PLQ55_04940"
     CDS             complement(144..>1463)
                     /locus_tag="PLQ55_04940"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HPK29311.1"
                     /translation="LVPVTMSSVERADMIKMYNPLLKDEFASKTKTFNLLKVAEALVG
                     QDVDKVVAMNLDFIDKFEEIVNDANFELIKAWMVVSNILTFSAELTEELRVAGGAFGR
                     ALQGIAEAQSQEKAAFYQAYDRLSQAVGLYYGLTYFGPEAKKDVEDMVNTIIGVYKDR
                     INKNTWLNAETKAKAILKLNTMKVHIGYPRELPKYYDKYKVGSYAEGSNLVKEKLKYS
                     RLVQKENFKKYNKKPNRNLWAMPASMVNAYYQPMNNQIVFPAAILQEPYYSLSQGRSA
                     NYGGIGAVIAHEISHAFDNNGAKFDEYGSLNNWWTDEDLKTFEEKAQKMIALFDGVES
                     GYGPCNGTLTVSENIADSGGIRCALEASKKEKDHNYEEFFKNWARVWRQKSSPEFMQL
                     LLTVDVHSPSNLRANMQLKNLVEFQEFYNLTEQDEMYLAKEQMVEIW"
BASE COUNT          424 a          293 c          236 g          510 t
ORIGIN      
        1 cgacctcttc cgtgtgaagg aaacgctctc ccatctgagc taagtgtccg gaagggacga
       61 ccttgtaata taatagcaaa atcatttata aatagcaatt aaaaaagagg gacaagccct
      121 cttagtttac aagatttgat tgtttaccaa atttcaacca tttgctcttt tgctaaatac
      181 atttcgtctt gttccgttaa attataaaac tcttggaatt caacgaggtt tttaagttgc
      241 atatttgcac gtaagtttga tggactatgc acatcaacag ttaagagtaa ttgcataaat
      301 tctggactag atttttgccg ccagacacgc gcccaatttt taaagaattc ttcataatta
      361 tgatcttttt cttttttgct tgcttctaag gcacagcgga ttccgccact atcagcgatg
      421 ttttcggaaa cggttaaagt accattacac ggcccgtatc ctgattcaac accatcaaat
      481 aatgcaatca ttttttgtgc tttttcttca aatgttttaa gatcttcatc tgtccaccag
      541 ttattaagtg agccgtattc atcaaacttt gcgccattat tatcaaaggc gtgcgagatt
      601 tcatgggcaa taactgcacc aattccgccg taattagcgc tacgaccttg acttaaacta
      661 taatatggtt cttgtaaaat cgcagcaggg aaaacgattt gattgttcat tggttgatag
      721 taagcattaa ccattgaagc tggcattgcc cataaattac gatttggttt tttattatat
      781 ttcttaaagt tttctttttg cactaaacgg ctatatttaa gtttttcttt cactaaattt
      841 gacccttcag cgtaactgcc aactttgtat ttatcataat attttggtaa ttcacgcgga
      901 taaccaatat gtactttcat ggtgttaagt ttaagaattg cttttgcttt tgtttcagcg
      961 ttaagccatg tgtttttatt aatccggtct ttataaacac caataatagt attaaccata
     1021 tcttcaacat cttttttagc ttctggccca aagtaagtta agccgtaata gagcccaaca
     1081 gcttgtgaaa gacggtcata agcttgataa aacgctgctt tttcttgtga ttgtgcttcc
     1141 gcaatacctt gtaaagcgcg gccaaaagca ccaccagcaa cccgtaattc ttccgttaat
     1201 tcagcgctga atgtaaggat gttagagaca accatccacg ctttaattag ttcaaaatta
     1261 gcgtcattaa caatttcttc aaacttatca ataaagtcta aattcattgc tactacttta
     1321 tcaacatctt gcccgactaa tgcttccgca actttgagta aattaaatgt ttttgtttta
     1381 ctggcaaatt catcttttaa taatgggtta tacattttaa tcatgtcggc tctttcaaca
     1441 ctcgacatcg tcactggcac tag
//
LOCUS       DAOPCD010000230         1455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_70764_length_1455_cov_3.717857, whole genome shotgun sequence.
ACCESSION   DAOPCD010000230 DAOPCD010000000
VERSION     DAOPCD010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70764_length_1455_cov_3.717857"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PLQ55_04945"
     CDS             <1..276
                     /locus_tag="PLQ55_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29312.1"
                     /translation="KESTKFQKVIAAISLLFLIFLLLRAFNSLFDNEIVLFTNNFFQI
                     GTFLALVLLMLTNGVGFLLIMYDRSYALVKESSNLDPLTRIYNRRFL"
     gene            273..719
                     /locus_tag="PLQ55_04950"
     CDS             273..719
                     /locus_tag="PLQ55_04950"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPK29313.1"
                     /translation="MNKAESYFKRAQQEDGYLSFLFIDIDFFKKVNDTYGHLFGDEVL
                     KVVAKTIAENIRPLDLCCRFGGEEFLIYLHDTSKEEAITIGNRIREVVQLLEFKQVKE
                     YKCTISVGVYSAKPNTETNLQQFIDNADQALYKAKASGRNCVMSFD"
     gene            742..>1455
                     /locus_tag="PLQ55_04955"
     CDS             742..>1455
                     /locus_tag="PLQ55_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPK29314.1"
                     /translation="MKTAMMTGHVTRFGDLEFALRLIKELGFEAVDLTMYDTFGGVAV
                     RNRELFTGDIIANAKRIKSIAEEINLPIVQAHTPFHVYRKDDEEYNAYMLETQKLCLQ
                     IAGIIGIPYVVIHPVMNADNEFNKKFFDELLPLAKKMNVVIATENMWEWQVGAIHCDY
                     STCSTPESFLSLLEYINHPNLKACVDVGHAQMFNGFDKSVSPKLMIEKLAHKYCVALH
                     LHDNDCVNDYHKIPFDGVIN"
BASE COUNT          473 a          214 c          278 g          490 t
ORIGIN      
        1 aaagagagca cgaagtttca aaaagtcatt gccgcaataa gtctgctctt ccttatcttc
       61 ctcttattac gagcatttaa ttccctattt gataatgaga ttgtactttt cactaataat
      121 ttcttccaaa ttggtacatt ccttgcctta gtgttattaa tgttaactaa tggcgtagga
      181 tttttactta taatgtatga ccgctcttat gcattagtga aagaaagttc taatttagat
      241 ccgttaacac gcatttataa tcggcgcttt ttatgaataa agctgagtcc tattttaagc
      301 gggcacagca agaagatggt tacttatcat ttctttttat tgatattgat tttttcaaga
      361 aagtaaatga tacttatgga cacttatttg gggacgaagt attaaaggtt gtagcaaaga
      421 caatcgcaga aaacattcga ccattagatc tttgctgcag atttggcggg gaagagtttt
      481 taatttattt acatgacact agtaaagaag aagcgatcac aatcggtaat agaattcggg
      541 aagtagtgca actattagaa tttaaacaag ttaaagaata taaatgtaca attagtgtcg
      601 gtgtatattc cgctaagcca aacaccgaaa caaacctcca acagtttatt gataatgctg
      661 accaagcttt atataaagca aaagcttctg gtcgtaattg tgtaatgagt tttgattaaa
      721 taaatataga ggagcaaagt tatgaaaaca gcaatgatga caggccatgt gacacgcttt
      781 ggtgatttag agtttgcttt acgacttatt aaggagttag gttttgaagc tgttgattta
      841 acaatgtatg acacctttgg tggtgttgct gttcgtaatc gtgagttatt tactggtgac
      901 ataattgcaa acgcgaaacg cattaaaagt atcgctgaag agattaatct tccgattgtg
      961 caagcacata ccccgtttca tgtttatcgt aaggacgatg aagaatataa tgcatacatg
     1021 ttagaaacac aaaaattatg tttacaaata gcgggcatca ttggcattcc ttatgttgtt
     1081 attcatccag tgatgaacgc tgataatgag tttaataaaa agttttttga tgaactactt
     1141 ccgctagcga aaaaaatgaa tgttgtcatt gcaaccgaga atatgtggga atggcaagta
     1201 ggagcaattc attgtgatta ctcaacttgt tcaactcctg aatcattttt aagcctttta
     1261 gaatatatta atcatcctaa cttaaaggcg tgtgttgatg ttggtcatgc gcaaatgttt
     1321 aatggttttg ataaaagtgt atcaccgaaa ttaatgattg aaaaacttgc tcataaatat
     1381 tgtgttgcac tgcatcttca tgataatgat tgtgttaatg attatcataa aattccgttt
     1441 gatggtgtaa ttaat
//
LOCUS       DAOPCD010000231         1455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_70799_length_1455_cov_2.410714, whole genome shotgun sequence.
ACCESSION   DAOPCD010000231 DAOPCD010000000
VERSION     DAOPCD010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70799_length_1455_cov_2.410714"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1281)
                     /locus_tag="PLQ55_04960"
     CDS             complement(73..1281)
                     /locus_tag="PLQ55_04960"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HPK29315.1"
                     /translation="MKNKKFKRVFVVVMDSVGVGAMPDAHLYGDAGSHTLKHTAESKK
                     NGLHVPNLEKYGLGNLDDIKGVKKVKNHPNSYVMKMLEASTGKDTMTGHWEFMGIHTE
                     KPFKTFTDTGFPQALIDELSAKTGYKIIGNYSASGTEIIKELGEEQMRDNSLIVYTSA
                     DSVLQIAAHEEVTGLDELYRCCEIAREICLRPEYLLGRIIARPYIGSDPSNFKRTANR
                     HDYALSPPEKTALNILDDNGITVSAIGKINDIYNGFGITQAQKTISNEDGMDKTIAQA
                     KANEFNGLIFTNLVEFDSEYGHRRNPVGYADAIERFDKRLGELMEVVTEDDLVLLTAD
                     HGNDPTWHGTDHTREMVPLIAISKAFTKGRVLEHRKSFADIGATILANYNLKLTPTLI
                     GEVIDELLTE"
BASE COUNT          422 a          290 c          249 g          494 t
ORIGIN      
        1 aaatcatgtg gccacatgat tttattgtgt gtctacattt agggtatcag tacaaagtga
       61 ggataatatg agttattcgg ttagtaattc gtcgattact tcaccaatta aggttggggt
      121 taactttaaa ttgtaattag cgaggattgt cgcaccaatg tcggcaaagc ttttacggtg
      181 ttcaagcaca cgccctttag taaatgcttt gctaatagca ataagcggta ccatttcacg
      241 ggtatggtca gtgccgtgcc atgttggatc gttaccgtgg tcagcagtta aaagcactaa
      301 atcgtcttca gtaacgactt ccattaattc accgagacgt ttatcaaagc gttcaatggc
      361 atctgcatag cctactggat tgcgtctatg accgtattca ctatcaaatt caacgagatt
      421 agtgaaaatt aaaccgttaa attcattagc cttcgcttgg gcaatcgttt tatccattcc
      481 gtcttcatta cttatagtct tttgtgcttg tgtaatgcca aaaccgttat aaatatcatt
      541 aattttaccg attgcactga cggttatgcc gttatcatca agaatattaa gtgccgtttt
      601 ttccggtgga gaaagtgcat aatcatgccg attagcggtc cgtttaaagt tgcttgggtc
      661 actgcctatg taaggacgag caataattct acctaaaagg tattcaggcc gtaaacaaat
      721 ttcacgggca atttcacaac agcgatataa ttcgtcgagt cctgttactt cttcatgcgc
      781 tgctatttgt aaaacactat ccgcggatgt ataaacaatt aagctattat cacgcatttg
      841 ttcttcaccg agttctttaa taatctccgt accacttgct gaataattac caatgatctt
      901 ataacctgtt ttcgcactta attcatcaat tagtgcttgc gggaaacctg tatcggtaaa
      961 tgttttaaac ggtttttccg tatgaatccc cataaattcc cagtggccag tcattgtatc
     1021 tttaccagtg ctagcttcta acattttcat gacatatgaa ttagggtggt ttttaacctt
     1081 tttaacacct ttaatatcat caagattacc taagccatat ttttctaaat taggaacatg
     1141 taatccattc tttttacttt ccgctgtatg ttttaaggta tgactaccag cgtcaccata
     1201 taaatgtgca tcaggcatag caccaacacc gacactatcc ataacgacaa caaagacgcg
     1261 tttaaacttc ttatttttca taatttaatc ctttcgattt caggtttatt ataacaaata
     1321 ataaaacatt atatatatgg ttaagcacta atccatttag cgtatatttt aagttcagtg
     1381 ttttcggcaa ttgtgggcgc tatgtcaaaa tcaaatttat tggtacattc actttccgaa
     1441 taccaaccat caaat
//
LOCUS       DAOPCD010000232         1454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_70838_length_1454_cov_3.473195, whole genome shotgun sequence.
ACCESSION   DAOPCD010000232 DAOPCD010000000
VERSION     DAOPCD010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70838_length_1454_cov_3.473195"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLQ55_04965"
     CDS             complement(<1..293)
                     /locus_tag="PLQ55_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HPK29316.1"
                     /translation="MLTKKEMRYLRGLSNKLKAQYQLGKNEITDSFLSLLEKALVAKE
                     IIKVHVLQSVDKEVTSLALEVAARLNATVVETKGKTFTLYRQNVKAPKIKFPL"
     gene            complement(298..1425)
                     /gene="yqeH"
                     /locus_tag="PLQ55_04970"
     CDS             complement(298..1425)
                     /gene="yqeH"
                     /locus_tag="PLQ55_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HPK29317.1"
                     /translation="MTENLKKIKKCYGCGVVLQSENEEEIGYVPSNVLKRDDVFLCQR
                     CFKTHHYSADKLHSPVVTLDFMNLIKDIKAKNALVVYVVDVFNFESSFNPEINTALSG
                     LDIILVANKFDLLPKSTNEAKLKDYVATKVEREGLNVKKIIVASSTRNYNIDEVLEEM
                     ANFRKKGDVYFIGAVSSGKSSLIKTLLRNFKNETSRYITTSIYPNTTLAVQQIPISET
                     TTIYDTPGIAINNSLLGLAEKEVVKVIVPRKEIKPRTYQLLAKQSILIGGIARFDYVD
                     GPFTGFTFYFAEGIKLTRTHIKLADRTFNNMIAHQKTRPISYLVTGVQDLDAFEFTTY
                     ENKKYDVSWNGLGFVSFKGSGRVVRIYAPRGVAISYNESKI"
BASE COUNT          428 a          280 c          238 g          508 t
ORIGIN      
        1 cataacggga atttaatttt cggcgcttta acattttgcc gatataaagt aaatgtttta
       61 ccttttgttt caacaacagt agcgttcagg cgtgccgcta cttctagcgc tagacttgtt
      121 acttctttat caacgctttg taagacatgg actttaatta tttctttagc gacaagtgct
      181 ttttctaaaa gacttaagaa actatcagta atttcatttt taccaagttg atattgcgcc
      241 ttaagtttat tacttaggcc acgtaaatag cgcatttctt tcttcgttaa catataatta
      301 aatcttactt tcgttataac ttatggcaac accacgcgga gcgtaaatgc ggacgacacg
      361 gccactacct ttaaaactaa caaagcctaa gccgttccaa ctaacatcat actttttatt
      421 ttcatatgtt gtaaattcaa aggcgtcaag atcttggaca ccggtgacaa gataagaaat
      481 cggtctagtc ttttgatggg caatcatatt attaaatgtg cgatcagcga gtttaatatg
      541 tgtacgcgta agttttatac cttccgcaaa atagaaagta aaaccagtaa aaggtccgtc
      601 aacataatca aaacgcgcaa tcccaccgat taaaatgctt tgtttagcga gtaattggta
      661 agttcttggc ttaatttctt tacgcggcac aattaccttt acgacttctt tttcggcaag
      721 gcctaagaga ctattattaa tcgcaatccc tggtgtatca taaatcgttg ttgtttcact
      781 gattgggatt tgttggaccg ctagtgtggt attaggataa atacttgtcg taatataacg
      841 actagtttca tttttaaagt tccgcagtaa tgttttaatt aaactagatt tcccgctgct
      901 gacagcacca atgaaataaa catcaccttt tttccggaaa ttagccattt cttcgagtac
      961 ttcatcaata ttatagtttc ttgtactact agcgacgata atctttttaa cattaagtcc
     1021 ttcgcgttca actttagtgg cgacatagtc tttaagctta gcttcatttg tcgattttgg
     1081 taataagtca aatttatttg cgactaaaat tatatctaga cctgaaagag ccgtatttat
     1141 ttctggatta aaacttgatt caaagttaaa gacatcaacg acatatacca caagggcatt
     1201 tttggcttta atatctttaa ttaagttcat aaaatcgagc gtgacaactg gtgaatggag
     1261 tttatcagcg ctatagtgat gcgttttaaa acagcgttga cataagaaaa catcgtcgcg
     1321 cttaagaaca ttacttggta catatcctat ttcttcttca ttttcacttt gaagcacgac
     1381 tccgcatcca taacactttt taattttctt taagttctct gtcatcttcg ttccactcct
     1441 ttaatttgcc cttg
//
LOCUS       DAOPCD010000233         1453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_70928_length_1453_cov_3.092990, whole genome shotgun sequence.
ACCESSION   DAOPCD010000233 DAOPCD010000000
VERSION     DAOPCD010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70928_length_1453_cov_3.092990"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1453)
                     /gene="thrS"
                     /locus_tag="PLQ55_04975"
     CDS             complement(<1..>1453)
                     /gene="thrS"
                     /locus_tag="PLQ55_04975"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017144149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPK29318.1"
                     /translation="DAAHLMAQAILSIYPGAKFGIGPDISEGFYYDVDFGDVVINDGD
                     LAVIEAKMMELSRADHRIERQVVSKEKALEIFAKNEYKVELINEIEERITIYSQGDFV
                     DLCYGPHMPSTRYVQHFKLLSLAGAYWRGDASRKQLTRIYGVAFFSKDALDKHLYNLE
                     EAKKRDHRKLGRELGLFMLSEYGPGFPFWLQKGMTLYHELEHWWIKKQREAGYEFVKT
                     PQILSRELWETSGHWLNYRENMYTTLIDERDFAIKPMNCPGSILIYNNGLHSYKDLPI
                     RMAEHGHVHRHEPSGSLNGLFRVRSFTQDDAHIYCRKDQLQSEIKRLLKLYDEVYKTF
                     NLSYHIELSTRPEKKYIGDLKTWNLSEKALAKACIASGIPFKINEGDGAFYGPKLDFK
                     LRDSLGRIWQCGTIQLDMNLPERFDISYIDSNNQKIRPIMLHRAVFGSIERFIGILIE
                     HYAGAFPLWLSPIQVAILPVNNEAHLKHAQSVAR"
BASE COUNT          438 a          294 c          267 g          454 t
ORIGIN      
        1 acgcgcaaca ctctgtgcgt gttttaaatg cgcttcatta tttacaggga gaatcgctac
       61 ttggattggc gataaccaaa gggggaaggc gccagcgtag tgttcaatta aaatgccaat
      121 aaagcgctca atcgaaccaa agaccgcgcg gtggagcata attgggcgaa ttttctgatt
      181 atttgaatcg atataagaaa tatcaaagcg ttcgggtaaa ttcatatcta attgaatcgt
      241 tccgcattgc caaattcgtc ctaaactgtc acgcaattta aagtcaagtt ttgggccgta
      301 aaaagcacca tctccttcat taattttaaa aggaataccg ctagcaatac acgctttcgc
      361 tagtgctttt tcgcttaaat tccatgtttt taagtcacca atatatttct tttcaggtct
      421 tgttgaaagt tcaatatgat aactaagatt aaatgtttta tatacttcgt cataaagttt
      481 aagcaaacgt ttaatttcac tttgtaattg atctttacgg caataaatat gcgcatcatc
      541 ctgagtgaaa ctccgcactc tgaataatcc gttaagtgaa ccgcttggtt catgacggtg
      601 gacatgacca tgttcagcca tccggattgg caaatcttta tatgaatgta aaccattatt
      661 ataaataagg attgaaccag gacaattcat cggtttaatg gcaaaatcgc gttcatcaat
      721 aagagttgtg tacatatttt cacggtaatt aagccagtgt ccgcttgttt cccatagttc
      781 acgacttaag atctgcggcg ttttgacaaa ttcgtaacct gcttcgcgtt gttttttaat
      841 ccaccagtgt tcaagttcgt gataaagagt cattcctttt tggagccaga aggggaagcc
      901 tggaccatat tcactaagca taaatagtcc aagttcgcga cctaatttac ggtggtcacg
      961 tttttttgct tcttcaaggt tatagagatg cttatcaagt gcatctttgg agaagaaggc
     1021 gaccccataa attctcgtta attgtttacg acttgcgtca ccacgccaat aggctcctgc
     1081 taaactaagc agtttaaaat gttggacata acgcgtactt ggcatatgtg gaccgtaaca
     1141 taaatcaaca aagtcacctt gcgaataaat agtaattcgc tcctcaattt cattaataag
     1201 ttcaacttta tattcatttt tagcaaagat ttctaatgct ttttctttac taacaacttg
     1261 gcgttcaata cggtggtctg ctctagagag ttccatcatt ttggcttcaa taaccgctaa
     1321 atcgccatcg tttataacaa catcaccaaa atcaacatca taataaaagc cttcactaat
     1381 atcgggacca atcccaaatt tagcgccagg ataaatactt aaaattgctt gtgccatgag
     1441 gtgggcagcg tca
//
LOCUS       DAOPCD010000234         1449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_71199_length_1449_cov_5.852224, whole genome shotgun sequence.
ACCESSION   DAOPCD010000234 DAOPCD010000000
VERSION     DAOPCD010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71199_length_1449_cov_5.852224"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLQ55_04980"
     CDS             <1..292
                     /locus_tag="PLQ55_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK29319.1"
                     /translation="ISKPKILILDEATSSVDTRTEYAIQSVMNQIMKDRTTFIIAHRL
                     STIKNAKKILVMNKGNIIETGTHEELLAANGFYAGLYNAQFLGIDDSQETVD"
     gene            complement(350..>1449)
                     /locus_tag="PLQ55_04985"
     CDS             complement(350..>1449)
                     /locus_tag="PLQ55_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPK29320.1"
                     /translation="ENEKDVWEKTAYYVNFSTYINYKLIGELIDSVANQTGHFPIHFK
                     KGKWYGDNALKNIYRVPRTKLAKIVKQGTVMGVISEAASKATGLPQGLKIIAAGTDKG
                     SEAFGTGAYRKDIASISYGTASSISVPNKKYIEPELFLPAYSAPIDGLYQMEVQIYRG
                     YWMIGWFIKQFGTHETLEAKIETMATEEVLNNKMLEIPPGSQGLVLQPYWGPGLKRPE
                     ARGAIVGFSDYHTRIHVYRAIIEGIAFGLKEGLLGIEKRQHKKVKEIRVSGGGSQSDA
                     ICQITADIFGVPVSRTQTYETATIGTALVTFYYLGVFDSLETATKAMVHIEKTFIPNP
                     KNVKQYNKLFKDVYAKLYPKLKGIYKKLKYF"
BASE COUNT          425 a          275 c          237 g          512 t
ORIGIN      
        1 tatttctaaa cctaaaatcc ttattcttga tgaagctact agtagtgttg atacgcgtac
       61 ggaatacgcg attcaaagtg ttatgaatca aattatgaaa gaccgcacta catttattat
      121 tgcccaccgc ttaagcacca ttaaaaatgc taagaagatt ttggtaatga ataaaggtaa
      181 cattattgaa acgggaactc acgaggaact tttagcagca aacggatttt acgctggtct
      241 ttataatgca caattccttg gtattgatga cagccaagaa actgtggatt aatcaattat
      301 ttacagcaaa aaagagttaa tttgtcgatt aaccctttta ttttttcttc tagaagtatt
      361 ttaatttctt atatatgcct tttaattttg gataaagttt agcgtataca tctttaaata
      421 atttattata ctgtttaaca tttttaggat ttggtataaa tgttttttca atgtggacca
      481 tcgcttttgt ggctgtttca agtgaatcaa acacacctaa ataatagaaa gtaacaagcg
      541 ctgtaccaat tgttgcggtt tcataagttt gtgtccgact aactgggaca ccaaatatat
      601 cagcggtaat ttgacatatc gcatcacttt gcgaaccgcc tcctgatact cttatttctt
      661 ttactttttt atgttgtctt ttttcaatac cgagtaaccc ttcttttaaa ccaaaagcaa
      721 tcccttcaat aatcgcccga taaacatgaa tgcgtgtgtg ataatcacta aatccaacaa
      781 tcgcaccacg agcttccggg cgtttaagtc ctggacccca atatggttgt aagacgagtc
      841 cttgtgagcc tggtggtatt tcaagcattt tattatttaa cacttcttca gtggccattg
      901 tttcaatttt ggcttcaagt gtttcatgcg taccaaattg tttaataaac cagccaatca
      961 tccagtagcc acgatatatt tgcacttcca tttgataaag accatcaatt ggtgcgctat
     1021 aggctggtaa aaataattcg ggttcaatgt attttttatt cggaacagat attgaacttg
     1081 cagtgccata ggaaattgaa gcaatatctt tgcggtacgc acctgttcca aatgcttcgc
     1141 ttcctttatc agttcccgct gcaatgattt ttaatccttg cggaagtcct gttgctttac
     1201 ttgcggcttc gcttataaca cccattaccg ttccttgttt tacaattttc gctaatttag
     1261 tgcgcggaac acgataaata ttctttaatg cattatcacc ataccatttc ccttttttaa
     1321 aatggattgg gaagtgacct gtttgattag cgacgctatc aataagttca ccaattaatt
     1381 tatagttaat gtaagtagag aaattaacat aatacgctgt tttttcccaa acatcttttt
     1441 cattttctt
//
LOCUS       DAOPCD010000235         1429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_72718_length_1429_cov_5.713246, whole genome shotgun sequence.
ACCESSION   DAOPCD010000235 DAOPCD010000000
VERSION     DAOPCD010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72718_length_1429_cov_5.713246"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   101..1428
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=167..185
                     /rpt_unit_seq="ctttaaatcccatcattgt"
BASE COUNT          449 a          359 c          187 g          434 t
ORIGIN      
        1 ccttatgaga ttttgaagca aaaaatgcaa ccgatttaac atcgtcaagc gaggtccatt
       61 tggtcacatg taataccagt tgttcgaggc gcaccagatg ctttaaatcc catcattgct
      121 tactaacacc tgatcgatgc caaaccggat tttgtcaata tcggcgcttt aaatcccatc
      181 attgtttact aacactcaca aaattaaccg acgatacctt ctccattccg gctttaaatc
      241 ccatcattgt ttactaacac tgcaaggtcg ctataaatta tttccgcttc ataatacttt
      301 aaatcccatc attgtttact aacacggatg agtagagttg aactaataca cggcgattgt
      361 actttaaatc ccatcattgt ttactaacac gggcgggtgg cgcctgcagc tcgacctata
      421 cgaatcgctt taaatcccat cattgtttac taacacagat agcaaaaccc attcgtaaaa
      481 tccacgaaaa gctctttaaa tcccatcatt gtttactaac acgaaagtaa aaacaacaaa
      541 agaatacaat aaaattatct ttaaatccca tcattgttta ctaacacgga gtataggggg
      601 actgccgcat aacatcaaac gtactttaaa tcccatcatt gtttactaac acagaaaaga
      661 cggtggattt tatatctaaa tatcagattc tttaaatccc atcattgttt actaacaccg
      721 aaaataataa aaatacacga gaacgatgct ttttacttta aatcccatca ttgtttacta
      781 acaccgctga agggatgacg ataatctgtg ttctgcttgc tttaaatccc atcattgttt
      841 actaacactt tttcaagata taatttccca gacataattc cacgccttta aatcccatca
      901 ttgtttacta acaccgggat tataagctcc cgatccgcta ttttccgaac tttaaatccc
      961 atcattgttt actaacacgc caaataaaag aggcttacgg cctcccctct aaaactttaa
     1021 atcccatcat tgtttactaa cacatccttt aagtgctatc caagatattg cgtcaccacc
     1081 tttaaatccc atcattgttt actaacactg cgctctgcgt gtttgtgcgg atgcgtcccc
     1141 cgcctacttt aaatcccatc attgtttact aacactcaca ccctgcgctg ttcgcaacgc
     1201 cccttgaatt tcctttaaat cccatcattg tttactaaca cccagatgaa agaaaaacac
     1261 actacgtaaa atttaaaact ttaaatccca tcattgttta ctaacacgag acggtgcaat
     1321 aagccaacgc ccgcttcccg aaatctttaa atcccatcat tgtttactaa cacccttgtc
     1381 taaatgcttc atggctgcgt cagaatcacc tttaaatccc atcattgtt
//
LOCUS       DAOPCD010000236         1426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_72947_length_1426_cov_2.998541, whole genome shotgun sequence.
ACCESSION   DAOPCD010000236 DAOPCD010000000
VERSION     DAOPCD010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72947_length_1426_cov_2.998541"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..351)
                     /gene="rpmG"
                     /locus_tag="PLQ55_04990"
     CDS             complement(202..351)
                     /gene="rpmG"
                     /locus_tag="PLQ55_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPK29321.1"
                     /translation="MRVNIQLRCTECNEENYLSSKNRKNNPERIEKMKFCPRCNKQTL
                     HREKR"
     gene            complement(429..1322)
                     /locus_tag="PLQ55_04995"
     CDS             complement(429..1322)
                     /locus_tag="PLQ55_04995"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015009864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HPK29322.1"
                     /translation="MSDKLILGSHVGMSAPDYYIGAINEALSYGANTFMIYTGAPQNT
                     RRLATEQMHALEGIKYLKEKGMDIKHIVAHAPYIINLGNSVKPETYELAVSFLQDELQ
                     RVADFGITMVVLHPGSHVGAGVEAGTEQIIKGLNTVLDNDTSGTIIALETMAGKGTEI
                     GRTFEEIRTIIDGVHKKSQLGVCLDTCHINDAGYDVHDIDGVLDEFDRVIGLEYLKVV
                     HVNDSKNERGAAKDRHENIGFGTIGYDTLMKYIYSPRLAHLPKILETPYINGVAPYGH
                     EITMIKNKVWNTTLKDELGEK"
BASE COUNT          396 a          298 c          224 g          508 t
ORIGIN      
        1 gctgttgtat gtttgttcgg taatcgcacc agtgcggaca tttttaactt taagtttcgc
       61 aatcatttta cgcattgctg tcttattaag taagatgtca agcacgttat aaatttcacc
      121 agcttcggta aaataattac cagggcgtaa ttctgtaaca ttaatcataa tttaaccctc
      181 ctctaattaa ttttaaatag tctagcgttt ttcgcggtgt agcgtttgtt tattgcaacg
      241 tgggcaaaat ttcatttttt caatccgttc gggattgttt ttcctgtttt tactggaaag
      301 ataattttct tcattacatt ccgtacatct taattgaata tttacacgca taattcttca
      361 tctcctctat ttgcttgtat atcatatcat aaatttagaa agtattacta aaagtaatat
      421 aattcgtctt atttttcccc taattcatct ttaagcgtag tgttccatac tttattttta
      481 atcattgtaa tctcatgacc ataaggtgcg accccattaa tataaggtgt ttctaagatt
      541 ttcgggaggt gtgctaagcg gggactataa atatatttca ttaaagtatc atagccaatc
      601 gtcccaaagc cgatgttttc atggcggtct ttagcggcgc cacgctcatt tttggaatca
      661 ttaacatgca cgacttttaa gtattcaaga ccaatcacac ggtcaaattc atctaagacg
      721 ccatcaatat catggacatc atatcctgca tcattaatat gacatgtatc aagacagacg
      781 cctagttggc tctttttatg aacgccatca ataattgtgc gaatttcttc gaaggtacgg
      841 ccaatttccg tgcctttgcc tgccattgtt tctaacgcta tgatcgtccc acttgtgtca
      901 ttatcaagca cagtattaag gcctttaata atttgttcag taccggcttc cacacctgca
      961 cccacgtgtg aaccaggatg aagcacaacc attgtaatcc caaaatcagc aacgcgttgt
     1021 aattcatctt gtaaaaaaga taccgctagt tcataagttt caggttttac actgttgcct
     1081 aaattaataa tataaggggc atgagccacg atatgcttaa tatccatccc tttttctttt
     1141 aaatatttaa taccttcaag tgcatgcatt tgctcagtcg ctaagcgtct tgtattttgc
     1201 ggtgcaccag tataaatcat aaatgtattt gcgccatagc ttaaagcttc attaattgcg
     1261 ccaatataat aatcgggagc actcatccca acatgactac ctaaaattaa tttatcactc
     1321 atgaatccct ccttttttcc gctttataac gttcgacacg ttgttcacgg attgactttt
     1381 taatcatctt acggcgatgt tttcttttta cattttccac cgctgt
//
LOCUS       DAOPCD010000237         1416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_73730_length_1416_cov_3.036738, whole genome shotgun sequence.
ACCESSION   DAOPCD010000237 DAOPCD010000000
VERSION     DAOPCD010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73730_length_1416_cov_3.036738"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PLQ55_05000"
     CDS             <1..264
                     /locus_tag="PLQ55_05000"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HPK29323.1"
                     /translation="HVESVAKEIGVKYTYDMLTAGGTDSGEIHKVHDGVINMTLSLPC
                     RYFHSHVSLVHSDDIDAAIDLLVAVIKGVDSKLIERLKATKYE"
     gene            361..1371
                     /locus_tag="PLQ55_05005"
     CDS             361..1371
                     /locus_tag="PLQ55_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29324.1"
                     /translation="MKLKKLLPLSFIFLVITSCDSGIKVSKQKANKAVFAAKEKSQFH
                     NFDSVNFTYIGEVLRGKVVYNLDKTTESEVSHFSLDVKAIATDIGSDDLKMAVNYIHY
                     EPSEDDENVFEPLYKRDNYYTEEWFYTKKESIPPPTNAGVSDKLNKKLKCKMGKLPAT
                     DVLYFFNNYYGSLLIERVPRGIAEYPGFLSIVNTVEARKTKDKLVVTYTMSKEELAFF
                     LKVSFGKNIDEMTSEEIEWYKTHPWYSENIERALNGLNITSHTTTIEINKDGLISRVT
                     NDINYTVTYYETTHSVIEKTTRHYKFKNEANFVFNSKTTITFPEFIDYLDLTNVYYPE
                     NT"
BASE COUNT          522 a          203 c          256 g          435 t
ORIGIN      
        1 catgttgaaa gtgtcgcaaa agaaattggc gttaaatata cttacgatat gttaactgct
       61 ggtggcactg atagtgggga aattcataag gtgcatgacg gtgtcattaa tatgacgcta
      121 tctctaccgt gccgctattt ccatagtcat gtctcacttg tgcatagtga tgacattgat
      181 gcagctattg atttactcgt cgcggtcatt aaaggcgttg atagtaagtt aattgaacgc
      241 ttaaaagcga ctaaatacga atagttaaat aatttaaagt gtaagagtac cgagggtaaa
      301 acttcggtac tttttattta tagaataaat agtggtacaa taaattatgg agtatgagtt
      361 atgaaactta agaaattatt gcctttatct tttatctttt tagttattac tagctgcgat
      421 agcggcatta aagtatctaa acaaaaagct aataaagcgg tatttgcggc gaaagaaaag
      481 tcacaattcc ataattttga ttctgtaaat tttacatata ttggggaagt attaagagga
      541 aaagtagttt ataaccttga taaaacgact gaaagtgaag taagtcattt ttcactagat
      601 gtaaaagcaa ttgcgaccga tataggaagc gatgacctaa aaatggccgt aaattatatt
      661 cattatgaac ctagtgaaga tgatgaaaat gtttttgagc cactttacaa gcgtgacaat
      721 tactacacgg aagaatggtt ctacaccaag aaagaatcaa taccaccacc aacaaatgct
      781 ggtgtaagtg ataagttaaa taaaaaactc aaatgtaaaa tgggaaaact tcccgcgacg
      841 gatgttttat atttttttaa caattattat ggttcattat taatagaacg tgttccgcgc
      901 ggaattgcgg aataccctgg ctttttatca atagtaaata ctgttgaggc gcgtaaaact
      961 aaagataaac tagtggtgac ttatacaatg tcaaaagaag aactagcgtt tttcttaaag
     1021 gtaagttttg gtaaaaacat tgatgaaatg acaagtgaag aaattgaatg gtataaaact
     1081 catccgtggt attcagaaaa cattgaacga gcactaaacg gtttaaatat tactagccat
     1141 acaacgacta ttgaaattaa taaggatggt cttattagta gagtcactaa tgacattaat
     1201 tatacagtta catattacga aacaacgcat tcggtaatcg aaaaaacaac aagacattat
     1261 aaattcaaga atgaagcaaa ttttgtattt aatagtaaaa ccactataac atttcccgaa
     1321 tttattgact atctcgatct tactaatgtg tattaccccg aaaacacgta aataaaaagt
     1381 gctgtcatta tttattcttc aaaaatatta attact
//
LOCUS       DAOPCD010000238         1387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_76184_length_1387_cov_2.903904, whole genome shotgun sequence.
ACCESSION   DAOPCD010000238 DAOPCD010000000
VERSION     DAOPCD010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76184_length_1387_cov_2.903904"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..73)
                     /locus_tag="PLQ55_05010"
     tRNA            complement(1..73)
                     /locus_tag="PLQ55_05010"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37..39),aa:Glu,seq:ttc)
     gene            complement(77..151)
                     /locus_tag="PLQ55_05015"
     tRNA            complement(77..151)
                     /locus_tag="PLQ55_05015"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:Asn,seq:gtt)
     gene            complement(218..442)
                     /locus_tag="PLQ55_05020"
     CDS             complement(218..442)
                     /locus_tag="PLQ55_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPK29325.1"
                     /translation="MTNLERIEQIFTELLKVEKIEPEMELKALGLDSLDLVEVMMRLE
                     EEFGIEFSNDEMLGFSTVADVVAEIERKTK"
     gene            complement(544..681)
                     /locus_tag="PLQ55_05025"
     CDS             complement(544..681)
                     /locus_tag="PLQ55_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29326.1"
                     /translation="MSELKGQILGLLLVVVIFATIAAAFKASVNNQLSEIASHESALV
                     I"
     gene            complement(698..1036)
                     /locus_tag="PLQ55_05030"
     CDS             complement(698..1036)
                     /locus_tag="PLQ55_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29327.1"
                     /translation="MSNIMSLVYSFILIFPTILLGVDFFIIGKIIVELETRATIVSYI
                     ISEEGGIRPSLVNELAEEGITITCEGECSYISVGETIKYQLIKTYTPIIIKNEDINIY
                     VTRTTIVGYL"
     gene            complement(1033..>1387)
                     /locus_tag="PLQ55_05035"
     CDS             complement(1033..>1387)
                     /locus_tag="PLQ55_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29328.1"
                     /translation="LMTTSGAMSALLESLINALENDHSVTPFINFSLPFKHPLVSQIM
                     INVYMLVNHGVDPKRLWQFNYLFETLVKEHNDEEIALHESSYERFNISLFIGTGIMIM
                     TLMISIMGMIGEYIV"
BASE COUNT          457 a          278 c          204 g          448 t
ORIGIN      
        1 gacccgtacg ggattcgaac ccatgaatgc atgcgtgaaa ggcatgtgag ttaaaccgct
       61 tctccaacgg gccagctggc gcctagtgta ggatttgaac ctacgaccga tcggttaaca
      121 gccgattgct ctgccgctga gctaactagg catgttagac ctaggcagtc ataagaatat
      181 cacacttcct aagtcttggc aataataatt attattatta tttagtttta cgctcaattt
      241 cagcgacaac atcagcgact gtggaaaatc caagcatttc gtcgttacta aattcaatac
      301 caaactcttc ttcgagtctc atcatgactt ccactaaatc aaggctgtca agtcctaatg
      361 cttttaattc catttcaggt tcaatttttt caaccttgag caattcggtg aatatttgtt
      421 caatacgttc taaatttgtc atagtattct ccttagaatt caaattttaa caaatagcac
      481 gcattatttc aaataaaaac gattccaagg tttcttcact gctaagcgac gaataaaagt
      541 taattaaatt actaacgcgc tttcgtgact agcaatttct gaaagttgat tattaactga
      601 tgctttaaag gccgcagcaa ttgtggcaaa aataacaaca acaagtaata accctaaaat
      661 ttgtcctttt agttctgaca taaaattacc tccttaatta taaataacca acaattgttg
      721 ttcttgtcac ataaatgtta atgtcttcat ttttaataat aatcggagtg tatgttttaa
      781 ttaattgata tttaattgtt tcccctacac tgatgtaaga gcattcgcct tcacaagtaa
      841 tcgttatgcc ttcttccgct agttcgttaa ctagacttgg cctaatgccc ccttcttcac
      901 taataatata gctaacaatt gttgcccttg tttctagttc aacaatgatt ttcccaatga
      961 tgaaaaagtc aacaccaagc aaaattgtcg ggaaaattag gatgaatgaa taaacaaggc
     1021 tcataatatt actcatacta tatattctcc aatcatcccc ataatactta tcatcagtgt
     1081 cataatcata attcctgtac caataaataa actaatgtta aagcgctcat aactactttc
     1141 atgtagcgct atttcttcat cattatgttc tttcactagt gtttcaaata aataattaaa
     1201 ttgccacaac ctttttggat caacaccgtg attaacaagc atataaacat taatcataat
     1261 ttgcgaaaca agcggatgct taaagggtaa ggaaaaatta ataaaagggg taacactatg
     1321 atcattttct aaagcattaa tcaatgattc taaaagagcg gacatcgcac cacttgtcgt
     1381 cattaat
//
LOCUS       DAOPCD010000239         1387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_76220_length_1387_cov_1.999249, whole genome shotgun sequence.
ACCESSION   DAOPCD010000239 DAOPCD010000000
VERSION     DAOPCD010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76220_length_1387_cov_1.999249"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PLQ55_05040"
     CDS             complement(<1..884)
                     /locus_tag="PLQ55_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPK29329.1"
                     /translation="MLLEFSISNFMSFEEKNTLSMIPSIQSNRGTFNISGINVLKFAT
                     IYGGNASGKSNLIKALSTLTYIVRLGLDERHRKLYCRVHEDNKDKPTIFSVTFYANEK
                     FYNYYFEVLLAERKVIFESLSELNPKTGNGKEIYTYTKGELDYKKVFNLKNIDEFNKN
                     RLKTYLFDNNMTQHKLLITDLISKEWDDSQLSFIKDVIHWFSHININMADFPIHDMKY
                     LEKVDERNKVEDILRELNTGVSKIDFTKISEDEFASRTNPHFAKAIIKDVKERYSEIV
                     KSGKKNIREFTLRGDSYYR"
     gene            complement(1110..>1387)
                     /locus_tag="PLQ55_05045"
     CDS             complement(1110..>1387)
                     /locus_tag="PLQ55_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HPK29330.1"
                     /translation="QLLKLSAAQLEYFKTGAQDETATLLTDARARYVAWSVYLGESNI
                     YGAGGGSPRIDINTEKDNTISVAVAIGVIGLSALLGFYFLQKKKKLA"
BASE COUNT          462 a          241 c          166 g          518 t
ORIGIN      
        1 cgataatatg aatcacctcg caaagtaaat tcgcgaatat tctttttacc gctcttcaca
       61 atttcactat atcgctcctt aacatcctta atgattgctt ttgcaaaatg aggattagtt
      121 cgcgaagcaa attcatcttc actaattttt gtaaaatcga tttttgacac accagtattt
      181 agttctctta aaatatcttc tactttattt cgttcatcaa ctttttctaa atatttcata
      241 tcatgaatgg gaaaatcagc catattaata ttaatatggc taaaccaatg aataacatct
      301 ttgataaaag ataattgact gtcatcccat tctttactaa ttaaatcagt aattaataat
      361 ttatgttgtg tcatattatt atcaaataaa taagttttaa gtctattttt attaaattca
      421 tcaatatttt taagattaaa tactttttta taatctaatt cccctttggt gtatgtataa
      481 atttctttac cgttaccagt tttagggttt aattcactta gcgattcaaa aatcactttt
      541 cgttccgcta aaaggacttc aaaataataa ttataaaatt tctcattagc gtaaaaagta
      601 acactaaaaa tagttggttt gtctttatta tcttcatgga cacgacaata taatttccga
      661 tgtctttcat ctaacccaag ccgtacaatg taagttagtg ttgatagtgc tttaattaaa
      721 ttactctttc ctgaagcatt accaccataa attgtcgcaa attttaagac attaattccg
      781 ctaatattaa aagtaccacg attactttga atacttggaa tcatacttaa tgtatttttt
      841 tcttcaaaag acatgaagtt actaatcgaa aattccaata acatatcact accctctttc
      901 tgtcttaata ttaccatgct cttttcactt ttacaatatt tttgtaattg ttctttatat
      961 tttcttatct actaattata aatacaatat atttttacat tttatctgta aatttattaa
     1021 aaataataat ttctgcattc gtactcattc atagtaataa taaaaagccg agcacaaagt
     1081 ctcggcttaa aactctagtt agtagagggt taggctagtt tctttttctt ttgtaagaaa
     1141 taaaagccaa gtaaggcact tagaccaata acaccaatcg ctaccgctac agagatagtg
     1201 ttatcctttt cagtattgat atcaatacgg gggctaccac cgccagcacc ataaatattt
     1261 gattcgccaa ggtaaactga ccaagcaaca taacgtgcac gtgcatctgt taataatgta
     1321 gcggtttcat cttgagcacc agttttaaag tattcaagtt gagctgcact taatttaagc
     1381 aattgtg
//
LOCUS       DAOPCD010000240         1380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_76748_length_1380_cov_3.292075, whole genome shotgun sequence.
ACCESSION   DAOPCD010000240 DAOPCD010000000
VERSION     DAOPCD010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76748_length_1380_cov_3.292075"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..447)
                     /locus_tag="PLQ55_05050"
     CDS             complement(91..447)
                     /locus_tag="PLQ55_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29331.1"
                     /translation="MRKITEFITITELAPLLSITRPTLYKYVVDYEAGDYRNIKYDIV
                     VIFDYIAKEAKNKVDIINFIKAQSEEKDSPLIKEIKALLKSDAAFKELLTFLVKHIRS
                     YEEALITLKEGEIKHE"
     gene            complement(614..1147)
                     /locus_tag="PLQ55_05055"
     CDS             complement(614..1147)
                     /locus_tag="PLQ55_05055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010289358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPK29332.1"
                     /translation="MRKIGIIVGSLRKDSYSGKVAKNIETLLKGKAEVKYIDISKLPL
                     YNQDYDGVIETPKAYVEFRSELSSVDAIVVVTPEHNRTMPAALKNALDVGSRPYGKNS
                     WKHKTYLVLSQSPGNLSGFGASNEVKMVLSFLNGRVMHQPEVYLANINTLLDEKGVFI
                     PDTLKFIDSVVAKFLEF"
BASE COUNT          411 a          237 c          203 g          529 t
ORIGIN      
        1 cccatttctt tttcgtgata ggcaagatca tttttaatcc gtaaaagatt aaatgaatac
       61 gtaatggttt gcacttcttt aatattaaaa ttattcatgc ttaatttctc cttcctttaa
      121 ggtaataagt gcttcttcat aacttcttat atgtttcact aagaaagtta ataattcttt
      181 aaacgctgca tcacttttta gtaatgcttt tatttcttta ataagcggac tatctttttc
      241 ttcactctgc gctttaataa agttaatgat atcaactttg ttttttgctt ctttagcaat
      301 atagtcaaat ataacgacaa tatcgtattt aatattgcga tagtcccctg cttcgtaatc
      361 aacgacatat ttatataagg ttggtcgcgt aatactaagt aagggcgcta gttcggtaat
      421 ggtaataaat tctgttatct ttcgcatagt tcctcctaga aacattatat gctggtgttt
      481 aaaatatgtc aagtcttttt tacacttatt ttacatattc tttgttatga ctgctttttg
      541 taatgtttta atgattgatt atataaaaaa aagcccgtca tcgacgggct taatactttg
      601 ttaagtttac ttattaaaat tcaagaaact ttgccactac tgaatcaatg aattttaagg
      661 tatctggaat aaagacgcct ttttcatcaa gtaaagtgtt aatgtttgct aagtatactt
      721 ctggttgatg catgacgcga ccatttaaga aacttaatac catttttact tcattagaag
      781 caccaaagcc acttaagtta ccaggtgact gacttagtac taaatatgtt ttatgtttcc
      841 atgagttttt accatatggc cgactgccaa catcaagggc gtttttaagt gccgccggca
      901 tggtgcggtt atgttctggg gtgacaacga caatcgcgtc aacactactt agttcactac
      961 gaaattcaac gtatgctttt ggtgtttcga ttacaccatc ataatcttga ttgtaaaggg
     1021 gaagcttact aatatcgata tatttaactt cagcctttcc ttttaataat gtttcaatgt
     1081 ttttggccac tttgcctgaa taactatctt tacgaagtga accaacgata attccaatct
     1141 ttctcatcta taattcctcc ttttttacct tattaattat acaatgacat ggctttttaa
     1201 aatggttatt tgcttacaga caaaaatcca taaaatttcc atgttaaata aataaaatag
     1261 ggttaggcta tttcttttac ttcatcttcc gcttttactt cattttctaa aataggagtt
     1321 tctgctttta cttttggttc aggaattact aagttaagaa atacagcaac aaacgtagcg
//
LOCUS       DAOPCD010000241         1378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_76910_length_1378_cov_3.811036, whole genome shotgun sequence.
ACCESSION   DAOPCD010000241 DAOPCD010000000
VERSION     DAOPCD010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76910_length_1378_cov_3.811036"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..>1378)
                     /locus_tag="PLQ55_05060"
     CDS             complement(85..>1378)
                     /locus_tag="PLQ55_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HPK29333.1"
                     /translation="ADYNYLFDPFVYLRRFFDSKRGNYIALVDEAHNLPRRVSDNYSL
                     VLDFTPFYSFRKVLKGAEHKKIRRIINRIVKDLDEYKEGIISEYETITEFPHELRSSL
                     DRFLVVGSEYMQEQGNNLAEGFSDFFFDVNKFIKILDLAYEDFILYTEIDEARKFFPK
                     FFIKCLSGAKFINEFLKVIDHAIFFSATLAPAPYFKTMLSIDDDIHQIPNPFSREQLL
                     IMVDGSTSLYYKDREQTLSVVAEKIRAAISVKTGNYIVYTPSFMYLENLEQELRNSED
                     YDLIVQRPSMSDIEKEKYLETFKLSPTKTTLGLAVLGGSFSEGVDLVSDRLSGVIILG
                     VGYPPPSFEKELEKDYFENKLNDGISYAYVYPALNKILQTMGRVIRSEKDYGFILLMD
                     KRYNYSPYRQQLESYDGLVVQVKSAERISYLLARFFLN"
BASE COUNT          452 a          270 c          205 g          451 t
ORIGIN      
        1 tttgttttta aaaatttaat ctttatttgt aaagataagt atgttatcta accaacattc
       61 atataagcac ttaaaaatag tgagttaatt taagaaaaaa cgcgctaaaa gataactgat
      121 tctttccgca ctttttactt gcacgactag gccgtcataa ctttctaatt gctggcgata
      181 gggactgtaa ttataacgct tatccatcaa aaggataaaa ccgtaatctt tttcacttct
      241 aattacgcgg cccatagttt gaagtatctt attaagcgca ggataaacat aagcataact
      301 tattccgtca ttaagtttat tttcaaaata atctttttct agttcttttt caaaagatgg
      361 tggcggatac cctactccta gaataattac accacttaag cggtcactaa caaggtcgac
      421 tccttcacta aaagagccgc ctaataccgc tagaccaagt gttgtttttg ttggcgagag
      481 tttaaatgtt tctagatact tttctttttc gatatcactc atacttggtc gttgcacaat
      541 taaatcatag tcttcgctat ttcttaactc ttgctccaaa ttttcaagat acataaaact
      601 gggcgtataa acgatataat taccagtttt aacacttatc gctgcccgaa ttttctccgc
      661 tactacactt agagtttgtt cgcggtcttt atagtaaaga ctagtgctac cgtcaaccat
      721 aattaaaagc tgttcacggc taaacgggtt aggaatttga tgaatatcat catcaatact
      781 aagcatcgtt ttaaagtaag gcgctggtgc aagtgtcgca ctaaaaaata tcgcgtggtc
      841 aattactttt aaaaattcat taataaactt tgcgccacta agacacttaa taaaaaactt
      901 tgggaaaaac ttccgtgctt catcaatttc ggtgtaaagg ataaaatctt catacgccaa
      961 atcgagaatt tttataaatt tattcacatc aaaaaagaaa tcgctaaagc cttccgctag
     1021 gttattgcct tgttcttgca tatattcact acctacaact aaaaagcggt caagactact
     1081 ccgtaattca tgtggaaatt cagtgattgt ttcatactca ctaataatcc cctctttata
     1141 ttcatcaagg tctttaacaa tccgattaat aattcgtcta attttcttat gttccgcgcc
     1201 ttttaacact tttctaaaac tatagaacgg cgtaaaatca agcactaaag agtaattatc
     1261 actcacacgg cgtggtaagt tatgcgcttc atcaacaagg gcaatataat taccgcgttt
     1321 actatcgaaa aagcgcctta aatacacaaa aggatcgaat agataattgt aatcagca
//
LOCUS       DAOPCD010000242         1365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_78086_length_1365_cov_2.003053, whole genome shotgun sequence.
ACCESSION   DAOPCD010000242 DAOPCD010000000
VERSION     DAOPCD010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78086_length_1365_cov_2.003053"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1365)
                     /locus_tag="PLQ55_05065"
     CDS             complement(<1..>1365)
                     /locus_tag="PLQ55_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPK29334.1"
                     /translation="LVEKDSDLASGQSSRNGGVVHTGLSYPKGSLRLKYCVEGNKMYK
                     AMCEDLHVPFYNTGQVTFARRPLEMLALRIAAKGGKKKGIDKLKILNREELLKYEPSV
                     PEWSIGGLYMGTGGITCPHTLTIALAENAVENGAEVSLDTAVIDMTVKDHNIVEVLTN
                     RGRIYPKVVINAAGVYADKIAELAFDRTFTIHPRSGTYLVTDKKIGHIVNGSMGKTPF
                     TLAPSTIAKLPKKHFAVLKATIKNLKSPSKGIGLIKTVDDNVLIGPNAKEVINREDYA
                     TERLVADTIFEVQQEVQPSLKKSDIIAYFTGVRAATYEEEFVVRQGIKTKNIFEVAGI
                     QSPGLTAAPAIAVDAAKWTVSFLEEQGFKVEENKTFNPKRKYQPILKHLSLEERDKLI
                     KENKDYGEIVCRCEEISKGEIIASLNSPIPVYTVDATKKRVRPGMGRCQGTFCLSHVM
                     KIIA"
BASE COUNT          371 a          296 c          233 g          465 t
ORIGIN      
        1 tggctattat cttcattaca tgtgaaagac agaaagtacc ttgacatctt cccatacctg
       61 gtctaactcg cttcttagta gcgtccactg tataaacagg aataggagaa ttaagtgacg
      121 ctattatttc cccttttgaa atttcttcac agcgacatac tatttcaccg taatctttat
      181 tttctttaat taatttgtct ctttcttcaa gacttaaatg tttaagtatt ggctgatatt
      241 ttcttttagg attaaatgtc ttattttctt ctaccttaaa tccttgttct tctaagaaag
      301 atactgtcca cttagcggca tctaccgcaa tcgcgggagc agcggttagt ccaggtgact
      361 ggataccagc aacttcaaag atgttcttag tcttaatgcc ttgtcttacc acaaattctt
      421 cttcataagt tgcagcgcgt actccagtaa aataagcaat gatatcactt ttctttaatg
      481 aaggttgtac ttcttgttgg acttcaaata tagtatccgc gacaagtctt tcagtagcgt
      541 aatcttctct gtttattact tcttttgcat tagggccaat taagacatta tcgtccactg
      601 ttttgattag accaatacct tttgaaggtg attttaagtt tttaatcgtg gctttaagta
      661 cagcaaaatg tttcttcggt aatttagcaa tggtactcgg tgcaagggta aagggcgtct
      721 ttcccatact accatttacg atatgtccga ttttcttatc agtaactaaa taggtgccag
      781 aacgcggatg aatagtaaac gtgcggtcaa atgcaagttc ggcaatctta tcagcgtata
      841 ctccagcagc gttaatgacg actttcgggt aaatccgccc gcgatttgtt agtacttcca
      901 ctatattatg gtcttttaca gtcatatcga taaccgcggt atctaaacta acttctgcac
      961 cattttcgac agcattttcc gctagtgcaa tagttaaagt atgcggacaa gtaataccgc
     1021 ctgtccccat atataaacca ccaatgctcc actctggaac ggacggttca tatttcaaaa
     1081 gttcttctct attaaggatt ttaagtttat caatcccttt tttctttccg cccttcgcgg
     1141 caatgcggag cgctaacatt tctagagggc gtctcgcaaa agtaacttga ccagtgttat
     1201 aaaagggcac atgtaaatct tcacacatcg ctttatacat tttgttgcct tcaacacaat
     1261 actttagcct aagtgaaccc ttaggataac ttagtcctgt gtgaactact ccgccattac
     1321 gacttgattg accacttgca agatcactat ctttttccac aagta
//
LOCUS       DAOPCD010000243         1344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_79889_length_1344_cov_3.858805, whole genome shotgun sequence.
ACCESSION   DAOPCD010000243 DAOPCD010000000
VERSION     DAOPCD010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79889_length_1344_cov_3.858805"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLQ55_05070"
     CDS             <1..191
                     /locus_tag="PLQ55_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HPK29335.1"
                     /translation="DTDFILSVINEVINENLNVIDDIKAGKDRAMGFLVGQVMKKTKG
                     KVNPALASKLMKQEIDKR"
     gene            249..755
                     /locus_tag="PLQ55_05075"
     CDS             249..755
                     /locus_tag="PLQ55_05075"
                     /inference="COORDINATES: protein motif:HMM:NF017941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HPK29336.1"
                     /translation="MKKKKIIRQITDNALIAAIYYVLTIILGGFAFNDVQFRISEILL
                     FLIFFRKDFVIGVTLGCLLANLHSLLQPWDVIFGTLATLLSGVLIAFSKRLYWGVIWP
                     TIINAVVVGLMLHIILDLPLFATMGLVAIGEFVVLLLGHAVFSLLAKQPAFLNLIMAE
                     QRKEEKDG"
     gene            748..>1344
                     /locus_tag="PLQ55_05080"
     CDS             748..>1344
                     /locus_tag="PLQ55_05080"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006526259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPK29337.1"
                     /translation="MAKKPPVYLEIIHECTQSGARYGFLHTPHGKVEVPMFMPVGTLA
                     NVKTITPEEIVALGSGVILANTYHLSLRPGTDILKKAGGIHSFMNHHGPILTDSGGFQ
                     VFSLADNRKISEEGVTFKSHLDGRELFFSPEEAIRMQEEIGADIIMSFDECIHYPADY
                     EYVKASTERTLRWAKRGKAAHKREDQALFGIVQGGEYPD"
BASE COUNT          415 a          225 c          289 g          415 t
ORIGIN      
        1 gtgatactga ctttatcctt agtgttatta acgaagtgat taatgaaaat ttaaatgtaa
       61 ttgatgacat taaagcaggt aaagatcggg ctatggggtt cttagtcgga caagttatga
      121 aaaagacaaa aggaaaagtt aatcccgctc ttgctagcaa actgatgaag caagaaattg
      181 ataagcgcta agcaagcgga gttgaggtgt tccttgccta accaccacca tcaaaacaag
      241 gagaaaatat gaagaagaaa aagattattc ggcagattac agataatgcg ctgattgctg
      301 ccatttatta tgtattaaca attattcttg gcggatttgc ttttaatgat gtgcaatttc
      361 ggatctcaga aatattatta tttttaatat tttttagaaa agactttgtc attggtgtta
      421 cactcgggtg tttgctcgct aatttgcata gtctgcttca accgtgggat gttatctttg
      481 ggacgcttgc tactttatta agtggagtgt taattgcttt tagtaaaaga ctatattggg
      541 gtgtaatatg gccgacaata attaatgctg ttgtcgtcgg actaatgtta cacattattc
      601 tcgatttacc attatttgct acaatgggac ttgtcgcaat tggtgaattt gttgtactct
      661 tattagggca tgctgtgttt tcgctcctag cgaagcaacc tgcatttcta aatctcatta
      721 tggctgaaca aagaaaagag gaaaaagatg gctaaaaaac cacccgttta tctagaaatt
      781 attcatgaat gcacacaaag cggtgcgcga tacggttttt tacatacacc gcacggcaaa
      841 gttgaagtgc cgatgtttat gccggtaggt acgcttgcta atgttaaaac aattactcct
      901 gaagaaattg ttgccttagg cagcggagtc atcttggcga atacttatca cttatcactt
      961 agaccaggca ctgatatttt aaaaaaggct ggtggaatcc actcttttat gaatcatcac
     1021 ggaccaattc ttactgattc aggcggcttt caagtattct ctttagccga taatcgtaag
     1081 attagcgaag aaggtgtgac ctttaaatca catttagatg gacgcgaact tttcttctca
     1141 ccagaagaag caattaggat gcaagaagaa attggtgcgg atattattat gagttttgat
     1201 gaatgtattc attatcccgc agattatgaa tatgtaaaag caagtactga acgtacttta
     1261 cgctgggcta agcgcggaaa agcagcgcat aagcgtgaag accaagcttt atttggtatt
     1321 gtacaaggcg gagaataccc cgac
//
LOCUS       DAOPCD010000244         1317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_82492_length_1317_cov_1.910460, whole genome shotgun sequence.
ACCESSION   DAOPCD010000244 DAOPCD010000000
VERSION     DAOPCD010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82492_length_1317_cov_1.910460"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="PLQ55_05085"
     CDS             <1..1059
                     /locus_tag="PLQ55_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29338.1"
                     /translation="KAPYAGVLLPLGFGEGPGQAGNMGDVYEKLTDYNALAGGRDFGL
                     SIAAMGFVVAAVVGTVILNVVARKGLVTRRKAEVTETFGGLADSQYAVVDHPEEIPVV
                     ESVDKFTIQVAFVIAIYLIAFGIMSLVSFLVVDLAKFASIRGIIWGFNFLFAIITTMI
                     VKAVLNLLRKKKIMKRKYINNFMQNRIAGLAFDVMIAAGIMSINFGKLNDPSFWVLLA
                     LMSVFGTIVSYYYINFVAKRYFPKTRWYTFFGFFGMLMGTMSEGVALLREIDPYFETG
                     VAEDLVNGSGTAALFGVPMLLITTVVYRGWVGFWVSFALLIVLFAAMILVLHFVTKSR
                     NKKLGAETNGEKEVLETE"
BASE COUNT          389 a          205 c          290 g          433 t
ORIGIN      
        1 aaagcacctt acgctggtgt cttattaccg cttggttttg gtgaaggccc tggtcaagct
       61 ggtaatatgg gtgatgttta tgaaaaatta acggattata atgcccttgc tggtggtcgt
      121 gactttggtc taagtattgc cgcaatgggc tttgttgtcg ccgctgttgt tggaacggta
      181 atcttaaatg tcgttgctcg caaagggcta gtaacacgaa gaaaagctga agttacagaa
      241 acttttggcg gtttagcgga tagtcaatat gcggtggtag atcatccaga agaaatacca
      301 gttgtggaat cagttgataa atttactatc caagtagcgt ttgttattgc tatttattta
      361 attgcatttg gtattatgtc actcgttagt tttcttgttg tagatttagc aaaatttgcg
      421 tctatacgcg gaattatttg gggatttaac ttcctttttg cgattattac gacaatgatt
      481 gttaaagcag tactaaattt attacgcaag aaaaagatta tgaagcgtaa atacattaat
      541 aactttatgc aaaatagaat tgctggttta gcctttgatg tgatgattgc cgcaggtatt
      601 atgtcaatta actttggtaa attaaatgat ccaagtttct gggtgttact cgcgcttatg
      661 agtgtatttg gcacgattgt cagttattac tatattaact ttgttgctaa gcgttatttc
      721 ccaaaaacga gatggtatac tttctttggt ttctttggga tgttaatggg aacaatgagt
      781 gaaggcgtcg ccttgctacg ggaaattgat ccatattttg aaacaggagt agcagaagac
      841 ttagttaacg gcagcggaac cgctgcgcta tttggtgtac cgatgcttct aattactact
      901 gttgtctatc gtgggtgggt tggattctgg gtatcttttg cccttctaat cgtcttattt
      961 gccgcaatga ttttagtgct gcattttgtt actaaatcac gcaataagaa actcggcgcg
     1021 gaaacgaatg gtgaaaaaga agtactagaa acagaataaa acaatttaaa actacaaata
     1081 agcactgcat ggcggtgctt attttttact aatttactca aatgattaca tttgtagtaa
     1141 aatatcaacg tacgctattt ttggacttat tagatgaatt gtcactagtc ctggaggatc
     1201 atatggaaca aaaatcaatt aaacaaacac tcgaacaact aaattcgtgt cgcgaaaatg
     1261 gtttaacgac ggaagaagca cttaaacgtc aagaagtgca cgggaaaaac gaattac
//
LOCUS       DAOPCD010000245         1315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_82654_length_1315_cov_2.732540, whole genome shotgun sequence.
ACCESSION   DAOPCD010000245 DAOPCD010000000
VERSION     DAOPCD010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82654_length_1315_cov_2.732540"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /gene="yqeK"
                     /locus_tag="PLQ55_05090"
     CDS             <1..1063
                     /gene="yqeK"
                     /locus_tag="PLQ55_05090"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HPK29339.1"
                     /translation="GSFDPLHNGHLLIARHAKREIGADLVLFLLSPSTVWKKVQTSFN
                     HRANMISAALKDEEGFELSRIEEGNEGKLNYTYLTFMRLRELYPHDELYLLLGEDQAM
                     AFDQWKHPELIAEQAKILVYKRKGSKVSNENFERFDMLEITGPLSSTSSTKVRNFESI
                     DVPSVVLEYIGNNKLYFSGLVAKRMSDERYYHSLSVASLSRLIAIANDVDPLKAFKAG
                     LFHDIGKEVPAAKSLKIMEEHFPKYLNMPLWSHHQFVGAYLMKEEYLEKDKKLIDAVK
                     YHATGHKKMSKLAKVIYAADKIEPSRGFDSSQYIAECVKDINKGFVIVLQANKDYLIS
                     KGKDIDNALTNACFKKYLK"
BASE COUNT          452 a          208 c          239 g          416 t
ORIGIN      
        1 tggtagtttt gatcccttgc acaatggtca tctcttaatt gcccgtcacg caaaacgcga
       61 aattggtgcc gaccttgtgt tatttttact ttcaccttca acagtgtgga aaaaggtgca
      121 aacgagcttt aatcatcgcg caaacatgat cagtgccgct ttaaaagatg aagaagggtt
      181 tgaactttcg cgtattgaag aagggaatga aggtaaatta aattatactt acctcacatt
      241 tatgcggcta cgtgagcttt atccgcacga tgaactatat ttactgcttg gtgaagacca
      301 agcaatggct tttgaccagt ggaaacatcc tgaactcatc gccgaacaag ctaaaattct
      361 cgtttataaa cgcaaaggtt ctaaagtatc aaatgaaaac tttgaacgct ttgatatgct
      421 tgaaattact ggtcccttaa gtagcacaag tagtacaaag gttcgtaatt ttgaaagtat
      481 tgatgtccca agtgttgtat tagagtatat tggcaacaat aaactttact ttagtggcct
      541 agtcgctaaa cggatgagtg atgaacgcta ttatcattcg ctttcagtcg ctagtcttag
      601 tcgcttaatt gctatcgcta atgatgttga tcccttaaaa gcatttaaag cagggttatt
      661 ccatgatata ggtaaagaag taccagcagc aaaaagttta aaaattatgg aagaacattt
      721 cccaaagtat cttaatatgc cactctggtc acatcatcag ttcgttggtg cttatttaat
      781 gaaagaagaa tacttagaga aagataagaa attaatcgac gctgttaagt atcacgcaac
      841 agggcataag aagatgtcca agttagcaaa agtaatttac gctgctgata aaattgaacc
      901 ttcccgcggt tttgatagta gtcagtatat tgctgaatgt gttaaggata ttaataaagg
      961 ctttgtgatt gtcttacagg caaacaaaga ttatttaata agtaagggca aagatattga
     1021 taacgcgtta actaatgcat gctttaaaaa gtatttaaaa taacctacta atattaaatt
     1081 gatgaagagc aggccaatga gcctgttttt ttatttttgg ttataaaatc gaaaaatata
     1141 ccactttcaa taaggtttat tatgtaaact aaatatgtaa aatagcgtga ttatacgatt
     1201 attaagttaa aaattatttg attatttatt taattaaact ttgcgcaaag caaagagtta
     1261 tccacaattt cacacatact aaataactac taattaatta ctacaattga ttatt
//
LOCUS       DAOPCD010000246         1309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_83243_length_1309_cov_4.259171, whole genome shotgun sequence.
ACCESSION   DAOPCD010000246 DAOPCD010000000
VERSION     DAOPCD010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83243_length_1309_cov_4.259171"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..412)
                     /locus_tag="PLQ55_05095"
     CDS             complement(110..412)
                     /locus_tag="PLQ55_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29340.1"
                     /translation="MDELVLFSNKSEAILFKAIGFLVHVVKDEEEITEILKELPKEVK
                     VIAYDTFFTSFFTEYTKNQKQLYPLYLPLPFNTEDEGKAISEMKESIRKSIGIDLL"
     gene            complement(416..1132)
                     /locus_tag="PLQ55_05100"
     CDS             complement(416..1132)
                     /locus_tag="PLQ55_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HPK29341.1"
                     /translation="MNNVYLKGVLDAKISNLLTESDFATFANLSGMDRLNFFINNELV
                     STSIVTNFEALCKLALSDLKEEVSLYTKNDPLYVNYFFAHDVIKKDQGALRDFYNETY
                     EKALQRKEEALVNYLDYTHALLNILTLVRGKKRGDTTASLLANYLKQGVLSEETFSAL
                     VNGDTHTIYAYLKNNFNIDASESESTVELEEKLDEYLFNKLKILAMDSDFKPTLIYYI
                     KRKQLQIERLRNIVYTKRNG"
     gene            complement(1125..>1309)
                     /locus_tag="PLQ55_05105"
     CDS             complement(1125..>1309)
                     /locus_tag="PLQ55_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29342.1"
                     /translation="LLNKKDAKYNFTLSKEPTHVAEGFLLSTPQYDLIFDFKEIVINY
                     QKKHEQRIYNELFKDE"
BASE COUNT          382 a          236 c          191 g          500 t
ORIGIN      
        1 caacaagcac aacgtcataa acttgcgctg ttttcatccc gcttgctacg acaagcggac
       61 cagatacttt aacaatttta ccagttactt ttgccatatt catttcctct tatagaagat
      121 caatcccaat tgattttcta atactttctt tcatctcact tattgcttta ccttcatctt
      181 ccgtattaaa tgggagcggt aaataaaggg gatataattg tttttgattt ttagtgtact
      241 cagtaaagaa cgatgtaaaa aaggtgtcat aggctattac ttttacttct tttggtaatt
      301 cttttaagat ttcagtaatt tcttcttcgt ctttgacaac atggactaaa aagccaattg
      361 ctttaaaaag aattgcttcg cttttattac taaataacac tagttcgtcc atcttttatc
      421 cgttcctttt cgtgtacaca atattgcgta acctttcaat ttgtaattgc ttacgtttaa
      481 tgtaataaat taatgttggt ttaaagtcac tatccatcgc tagtattttt aacttattaa
      541 ataagtattc atctaacttt tcttctaact ccactgttga ttcgctttca ctagcgtcaa
      601 tattgaagtt gttttttaaa tacgcataga tagtgtgagt atcaccattt actaaagcgc
      661 taaaagtttc ttcacttaac acaccctgtt ttaagtaatt cgctaataaa ctcgcggtgg
      721 tgtcgcctct ttttttacca cgcactagtg ttaatatgtt taagagcgcg tgcgtataat
      781 ctaaataatt tactaaggct tcttcttttc tttgtagcgc tttttcatat gtttcattat
      841 agaaatcacg aagtgcacct tggtcttttt taataacatc atgggcaaag aaataattaa
      901 cgtataaggg gtcgtttttc gtataaagac taacttcttc ttttaaatca ctaagcgcga
      961 gcttacatag cgcttcaaag ttcgtaacga tacttgtcga aacaagttca ttattaataa
     1021 agaagttaag tctatccata ccgctaaggt ttgcaaaagt tgcaaaatca ctctctgtta
     1081 agagatttga tattttagcg tcaagtactc cctttaaata aacgttattc atctttaaat
     1141 aattcgttat aaatcctttg ctcgtgtttc ttttgatagt taatgacgat ttctttgaag
     1201 tcaaagatta agtcatactg cggtgtactt agcaaaaagc cttccgcaac atgtgtcggt
     1261 tctttactta atgtaaagtt atatttggca tctttcttat ttaataagt
//
LOCUS       DAOPCD010000247         1306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_83542_length_1306_cov_4.148681, whole genome shotgun sequence.
ACCESSION   DAOPCD010000247 DAOPCD010000000
VERSION     DAOPCD010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83542_length_1306_cov_4.148681"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..>1306
                     /gene="gpmI"
                     /locus_tag="PLQ55_05110"
     CDS             100..>1306
                     /gene="gpmI"
                     /locus_tag="PLQ55_05110"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPK29343.1"
                     /translation="MNKRPIILTILDGYGYSEETKGNAVKKAKTPNINAFYKNNPTVL
                     IEASGEAVGLPAGQMGNSEVGHQNIGAGRIVYQSLTYINQLIKDGRFFENEVFVNAVK
                     HTLKYNSKLHLMGLVSDGGVHSHINHIKAFLDLAKMHGAKEVYLHALMDGRDVAPQAG
                     PEYIQELVDYMAKINFGKLATIGGRYYGMDRDKNLNRTNVHYDAIVNRKGLFFSDFRE
                     YFKEQYETLPKNGFDPSDEFLVPGINKNVNGQLGDNDAVIFFNFRPDRAIQFGTIVTN
                     PYFYQNPPLKDGKPEYKAFVPSPILKNIYYACTMKYADSVHGHIALSLPKLDNGLGVF
                     LADKGYTQLRIAETEKYAHVTFFLDGTVNYDGVEKPELKGARRILVPSPKVATYDLQP
                     EMSAPGVTDA"
BASE COUNT          443 a          231 c          246 g          386 t
ORIGIN      
        1 aaggttaaaa cgccttacta aaatatagac aattaagcat ttttacacac aaagggccat
       61 tatagtaata taattactat cggaaagagg ttttttatta tgaataaacg tcctattata
      121 cttacaatct tagatggcta cggctatagc gaagaaacaa aagggaatgc cgttaaaaaa
      181 gcaaaaaccc caaacattaa cgcattctat aaaaataatc cgaccgtcct aattgaagcc
      241 tcaggcgaag cggtaggttt acccgctggg caaatgggaa atagtgaagt cggtcaccaa
      301 aatattggtg caggccgcat tgtttatcaa tcactaactt atattaatca actaattaaa
      361 gatggtcgct tttttgaaaa tgaagtattt gttaatgccg ttaaacatac acttaaatac
      421 aattctaaat tacatttaat gggtttagtt agtgatggcg gtgttcattc acatattaat
      481 cacattaaag cctttttaga tttagcaaaa atgcatggcg ctaaagaggt ttatttacac
      541 gctttaatgg atggacgcga tgtagcgccc caagctggcc cagaatatat ccaagaatta
      601 gtggattata tggcgaaaat taattttggt aaactcgcta ccatcggtgg ccgttattac
      661 ggaatggatc gtgataagaa tttaaatcgt acgaatgtcc attacgatgc gattgtgaat
      721 cgtaagggct tattctttag tgattttaga gaatatttca aagaacaata cgaaacatta
      781 cctaaaaacg gctttgatcc aagtgatgaa tttttagttc ccggaattaa taaaaatgtc
      841 aatggccaat taggagataa tgacgctgtt attttcttta atttccgtcc agatcgtgcg
      901 attcaatttg gaacgattgt cactaatcca tatttctatc aaaacccacc actaaaagat
      961 ggaaaaccag aatataaggc atttgtgcca agcccaatct taaaaaatat ttattacgca
     1021 tgtacgatga aatatgccga ttcagttcat ggacatatcg ctttatcact acctaaactt
     1081 gataatggct taggtgtctt tttagcggat aaaggttaca cacaactacg aatcgctgaa
     1141 acagaaaaat atgcgcatgt tacatttttc ttagatggta cggttaatta tgacggtgtt
     1201 gaaaagcctg aacttaaagg tgcgcgtcgt atcctagtgc cgtcccccaa agtagcgact
     1261 tatgacttac aaccagaaat gtcagcgcca ggagtcacgg atgctt
//
LOCUS       DAOPCD010000248         1305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_83640_length_1305_cov_4.012800, whole genome shotgun sequence.
ACCESSION   DAOPCD010000248 DAOPCD010000000
VERSION     DAOPCD010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83640_length_1305_cov_4.012800"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="PLQ55_05115"
     CDS             complement(<1..1268)
                     /locus_tag="PLQ55_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004462370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPK29344.1"
                     /translation="MALSIMLLVGFLLTRLTKPLKLPNVTAYIFAGIILGPLAQLILG
                     GKPLFPDELRKGMEFITDLALAFIAFGVVRYFKFDILKKSGPKILIITLFESLMAVAF
                     SFGAMMLMNLIPGVKMDWELAILLAAIASATAPASTMMTIRQTGAKGDFVNTILQVVS
                     LDNAIALILFSVAMAIITGNKESGGINFMQLAKPILLNLALIVAGYLMGLLLYWVTPP
                     KRTKDNRLIIAVAFMLGFSGVASLLDISPLLGVMAMGMAYTNIAPDESLFRQIDAFSP
                     PILTIFFVLSGMRLQLHMLASIGVVGVVYFIVRIIGKYTGATLGSIVARSTENNKKYL
                     GLALIPQAGVSLGLAALGSRILMGYGHNDYADMLTTIIVAAGVLYEIFGPPAAKYSLS
                     ITKSYTTEKTVEMIGPKPSEIPVSAEPKEVP"
BASE COUNT          416 a          288 c          258 g          343 t
ORIGIN      
        1 ggtacttctt taggttcagc cgaaacaggg atttcgcttg gttttggtcc aatcatttcg
       61 accgtctttt cggtcgtata agacttcgta attgagagtg agtacttcgc tgctggtggt
      121 ccaaagattt cataaagcac accggcggca acgataatag tcgtcaacat atcagcgtaa
      181 tcattatggc catatcccat taaaatccgt gaaccaagcg cggcgagccc tagactaaca
      241 cccgcttgcg ggattaaagc aagacctaag tacttcttat tattctcggt gctacgcgcc
      301 acgatactac caagcgtcgc acctgtatac ttaccaataa tacggacgat aaagtaaaca
      361 acacctacaa caccgatact agcaagcata tgtaattgta accgcatccc acttaatacg
      421 aagaaaatcg tcagaatcgg tggtgaaaag gcatcaatct gccggaaaag tgattcgtct
      481 ggtgcaatat tagtgtaagc catccccatt gccataacgc ctaaaagtgg gctgatgtct
      541 aagagtgaag cgaccccgct aaagccaagc ataaaggcaa cagcgataat aagccggttg
      601 tctttcgttc tttttggcgg tgttacccaa tataaaagta aacccattaa gtacccagcg
      661 acaattaaag cgaggtttaa aagaataggt ttagcaagtt gcataaagtt aatgccccca
      721 ctttctttat taccagtaat aattgccatc gccacactaa aaagaattaa agcaatggcg
      781 ttatcaagcg acactacttg taagattgta ttaacaaagt cccctttagc gcctgtttgt
      841 ctaattgtca tcatcgtgga agcaggtgct gtagcgctag caatcgctgc gagtaaaatc
      901 gctaattccc agtccatttt aactccggga attaagttca tcagcatcat cgctccaaag
      961 ctaaaagcaa cagccattaa gctctcaaat aatgtaatta tgagaatttt tggtccgctt
     1021 ttctttaata tgtcgaattt aaagtaacgc acaacaccaa aagcaataaa cgctaaagca
     1081 aggtcagtaa taaactccat tcctttacgg agttcatcgg ggaataaggg tttaccacct
     1141 aaaataagtt gcgcaagtgg tccaagaata ataccagcaa aaatataagc cgtcacgttt
     1201 ggtaacttta ggggttttgt gagccgcgtt aaaagaaagc caacaagtaa cataatgctt
     1261 aaagccatca gtactgttgg tacacttgtg tctttaaacg gactt
//
LOCUS       DAOPCD010000249         1293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_84830_length_1293_cov_3.277060, whole genome shotgun sequence.
ACCESSION   DAOPCD010000249 DAOPCD010000000
VERSION     DAOPCD010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84830_length_1293_cov_3.277060"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1014
                     /locus_tag="PLQ55_05120"
     CDS             91..1014
                     /locus_tag="PLQ55_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPK29345.1"
                     /translation="MKYEKISYFVSKWGITERRIRVLCTEGRIEGAVKVGKTWLIPKD
                     ASKPRDKRYKNINDLFFDDINTDAIDNKKAIIDESRPFSQTMIRQLNDKLLVEWTYHS
                     NAIEGNTLTMSETKVVLENGITIKGKPLKDHLEAINHKEAIEYVEELVKKDTKLTEYD
                     IKSIHYLILKEIDNLNAGKYRAQNVLISGSKHIPPSYINVPLEMQMLMEQYEDWAVYH
                     PLIRACLLHGEFVKIHPFVDGNGRTARLLLNFELIKNGYPPVIIKNEDRADYYDALEM
                     ASTTKNYTLFINLVLNLVNEAADSYLLLLGK"
     gene            974..>1293
                     /locus_tag="PLQ55_05125"
     CDS             974..>1293
                     /locus_tag="PLQ55_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
                     /protein_id="HPK29346.1"
                     /translation="MKPLTVICYCSVSNNKYITELKSSKVSHSTTKYKREVWQCNEKF
                     ENGKKGNKCSTPHLFEREIKEAFIKAFNLLCQKKNSVLNNCKDFITILNNTKELDEKI
                     MVQET"
BASE COUNT          486 a          189 c          231 g          387 t
ORIGIN      
        1 ttgcgatatc ggtataatgt tgtcttattt ctttgaaatg ctataatatt attgtgccga
       61 tatcggtata ataacattgg aggtgattat atgaaatatg aaaaaatatc gtattttgtc
      121 tcaaagtggg gtattacaga acggcgaata cgcgtgcttt gtaccgaagg aagaattgag
      181 ggcgcagtaa aagttggcaa aacatggtta attcccaaag atgcatcaaa accacgtgat
      241 aaacgttata aaaatatcaa tgacttattt tttgatgata taaatactga tgctattgat
      301 aataaaaaag caataataga tgaatcacga cctttttcac aaacgatgat ccgacaatta
      361 aatgacaagc ttcttgtcga gtggacatat cactcaaatg cgattgaagg caatacttta
      421 acgatgtcag aaacgaaagt tgtgctagaa aacgggatta caattaaagg caaaccatta
      481 aaagaccatc ttgaagcaat caatcataaa gaagcaattg aatatgttga agaactagtg
      541 aagaaagaca ctaaactcac agaatacgac ataaaatcaa ttcattattt aattttaaaa
      601 gagattgata atttaaatgc tggcaaatat cgcgcccaaa acgttttaat aagtggttca
      661 aaacatattc cgccctccta tattaatgtg ccgcttgaaa tgcaaatgtt aatggaacaa
      721 tatgaagatt gggcagttta tcatccgctt attcgcgcct gtttactgca cggtgaattt
      781 gtgaaaattc atccatttgt tgatggtaac gggcgaactg cgcgcttact tttaaatttt
      841 gaactaatta aaaatggcta tccacctgtg attattaaaa atgaagatcg cgctgattat
      901 tatgacgctc ttgaaatggc tagtaccacg aaaaattaca ctctttttat taatcttgtg
      961 cttaatttag ttaatgaagc cgctgacagt tatttgttat tgctcggtaa gtaataataa
     1021 gtacatcacc gaactcaaga gttcaaaagt ttcgcattca acaactaaat ataaaagaga
     1081 agtgtggcag tgtaatgaga aatttgaaaa tggtaaaaaa gggaataaat gtagtactcc
     1141 tcatttattt gaaagagaga ttaaagaagc atttattaaa gcctttaact tactttgtca
     1201 aaagaagaat agcgttttaa ataattgtaa agacttcatc acaatactta ataatacaaa
     1261 agaactagat gaaaaaataa tggttcaaga gac
//
LOCUS       DAOPCD010000250         1287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_85472_length_1287_cov_2.300325, whole genome shotgun sequence.
ACCESSION   DAOPCD010000250 DAOPCD010000000
VERSION     DAOPCD010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85472_length_1287_cov_2.300325"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /gene="priA"
                     /locus_tag="PLQ55_05130"
     CDS             <1..1181
                     /gene="priA"
                     /locus_tag="PLQ55_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPK29347.1"
                     /translation="VAQMRAKSFNAKVLVGSATPSIETMARARKEVYGYYYLGERINL
                     QPLPETTVVNMLDYKNVTTTSPYISKLLATEIRKAIDAKTQVILLVNRRGYATFVSCR
                     ECGESKKCPACAISLTYHKVGNNLKCHHCGLSFPKNDPCIKCGSTNFSYTGFATQKAE
                     EDIQKMFPDARILRLDSDVSRKDKTPQVVLNKFMNHEADILIGTQMVSKGHDFPLVSL
                     VGVIGTDANLSIPSFRSSERTFQLITQAIGRSGREKIKGKAIIQTMMPEHYVIQHAKQ
                     QDYFGFYEVEIRHRYLTSNPPFYFLLSITMSHQNERTVKDTILTLKLNLIKALGEDAT
                     VIGPTATFYPASGKTFNETLIVRYKNYFKIKPALLTTLSPFREQSSLYVHINVDPYDV
                     "
BASE COUNT          449 a          217 c          233 g          388 t
ORIGIN      
        1 ctgtagcgca aatgcgggcg aaaagcttta acgctaaagt attagttggt agtgcaacac
       61 caagcattga aacgatggcg cgcgctagaa aagaagttta tggttattat tacctaggtg
      121 aaagaattaa cttgcagccc ttaccagaaa cgacagttgt taatatgtta gactataaaa
      181 atgttaccac tacttcacca tatatttcta agttattagc gaccgaaatt agaaaagcta
      241 ttgatgccaa gacacaagtg attttattag taaaccggcg ggggtacgcc acttttgtaa
      301 gttgtcgtga atgtggcgaa tctaaaaagt gccctgcctg tgcgatatca ctcacctatc
      361 ataaagtagg aaataaccta aaatgccatc actgtggcct ttctttccct aaaaatgatc
      421 cttgcatcaa atgtggtagc actaatttct catatactgg atttgccaca caaaaagctg
      481 aagaagatat tcaaaaaatg tttcctgatg cacggatctt gcggcttgat agtgatgtga
      541 gtcgtaaaga taaaacacca caagtagtgt taaacaaatt tatgaaccac gaagcagata
      601 ttttaattgg tacgcaaatg gtttcgaaag gtcatgattt cccattagtt agtttagtgg
      661 gagtaattgg cactgatgct aatttatcaa taccctcatt tagaagtagt gaacgaacat
      721 ttcagctaat cacccaagcg attggtagat caggtcgcga aaaaataaaa gggaaggcga
      781 ttattcaaac aatgatgcca gaacattatg ttattcaaca tgcaaaacaa caagattatt
      841 ttggatttta tgaagttgaa attagacatc gttatttaac aagtaatccg cctttttatt
      901 ttttattatc aattacgatg agccatcaaa acgaaagaac ggtaaaagat actatattaa
      961 cattaaaact aaatcttatt aaagcactgg gtgaagatgc gactgtgatt ggtccaaccg
     1021 ctacttttta cccagcaagt ggtaaaacct ttaacgaaac acttattgtg cggtataaaa
     1081 attattttaa aattaaacct gcacttttaa ctacattaag tccatttaga gaacaatctt
     1141 ctctttatgt acatataaat gttgatccat atgatgtata gaaaggaaaa atattatggt
     1201 cattattaaa atttattctc aagacattta tgtcgctgct gaaatcagtg ttaaatacgc
     1261 tgatgctatt gctaaaacac taggttt
//
LOCUS       DAOPCD010000251         1286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_85575_length_1286_cov_2.238830, whole genome shotgun sequence.
ACCESSION   DAOPCD010000251 DAOPCD010000000
VERSION     DAOPCD010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85575_length_1286_cov_2.238830"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PLQ55_05135"
     CDS             complement(<1..535)
                     /locus_tag="PLQ55_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29348.1"
                     /translation="MKRKGSLLILIIPMLFSLTACNSGLNEYRENAKAEIARFVTDWQ
                     HIFNDHQEALNIAVQKGEEAINKARNKKEIDSIVKNTKADIDALLINDDYEFSISTEM
                     VVKKSGEDFSVEVKLKNNSGADIEMSYFWSPFWPFAPRWQYPLFIEPSEFPETVTFLN
                     NETYTETWTLGANFEVGK"
     gene            complement(674..>1286)
                     /locus_tag="PLQ55_05140"
     CDS             complement(674..>1286)
                     /locus_tag="PLQ55_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPK29349.1"
                     /translation="EVHGYYDFNLKEFKVTSREFTEAADIKRDYTEDTELLNLFNEKY
                     AEEIERVKGEVIGSLTNYINKSTLVKLANKVMYNFASITHNPDTIIAVHNYGGVRDGI
                     DAGEVTYGDIYKAFPFDNEIKMFEDVLGSTASSFIGSNASYPIGKFDIQNDQEYTIVT
                     IDYVSDSKYSNIRDYPHTVTGEYVRELIAEYFRTNGEIDPYTI"
BASE COUNT          408 a          237 c          178 g          463 t
ORIGIN      
        1 gctttccgac ttcaaaatta gcgcctaaag tccatgtttc cgtgtatgtt tcattattta
       61 agaaagtaac tgtttcagga aattcactag gctcaataaa aagtgggtat tgccatctag
      121 gggcaaaggg ccaaaatggt gaccaaaaat atgacatttc aatatctgcg ccactattat
      181 tttttaattt aacttcaaca ctaaaatctt caccgctttt tttaacaacc atctctgttg
      241 atatactaaa ttcataatca tcatttatta aaagagcatc aatatccgct tttgtatttt
      301 ttacaatact gtcaatttct tttttattcc ttgctttatt aatagcttct tccccttttt
      361 gtacagctat atttaaagct tcttgatgat cattaaagat atgctgccaa tcagtgacga
      421 aacgcgctat ttcagcttta gcattttcgc gatattcgtt gagaccgcta ttacacgcag
      481 ttagactaaa aagcattgga attataagaa taagtaaact tccttttcgt ttcataagat
      541 gtcacagcct ttcattttag ttataatttg cggcaatatt aaagttttat ctctaccctt
      601 aatttattac aaggtgggga atttacaata cgaaaaaagc tataaagtat tctaataaaa
      661 ttaatttatc tatttaaata gtgtaagggt ctatttcacc gttagtgcgg aaatattcag
      721 caattaattc acgaacatat tcaccagtaa cagtatgtgg gtaatcacga atatttgaat
      781 acttgctatc actaacataa tcgatagtaa cgattgtata ttcttgatca ttttgaatat
      841 caaatttacc gattggataa gaagcattgc taccaatgaa acttgaagca gttgagccta
      901 aaacatcttc aaacatctta atttcattat caaacgggaa ggctttataa atatcgccat
      961 atgttacttc tcctgcatca atcccgtctc gcacaccacc ataattatgg acagcaataa
     1021 ttgtatcagg gttatgcgta atactcgcga agttatacat caccttatta gcgagtttta
     1081 caagtgtact cttattaata taatttgtca gtgaacctat aacttcacct tttactcttt
     1141 caatttcttc agcatacttt tcattaaata agtttaataa ttcagtgtct tctgtgtaat
     1201 ctcttttaat gtccgctgct tcggtaaatt cacggctagt aactttaaat tcttttaagt
     1261 taaagtcata atagccatgc acttcc
//
LOCUS       DAOPCD010000252         1276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_86561_length_1276_cov_3.650287, whole genome shotgun sequence.
ACCESSION   DAOPCD010000252 DAOPCD010000000
VERSION     DAOPCD010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86561_length_1276_cov_3.650287"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1276)
                     /locus_tag="PLQ55_05145"
     CDS             complement(<1..>1276)
                     /locus_tag="PLQ55_05145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK29350.1"
                     /translation="VSAIATTFATFFFGTLSDRAGKRKPFISIGYIIWGIFTIAFGLT
                     GLLTKFTMNIWALATVIVLADTVMSFFGSMGNDAGFNAWTADLISDDNKGAIGTALAV
                     QPVIGTIIGTVLGGILIEAFGYIAFFSMMGGFVSLIGVLSLFLLKDSPKLEPSKRGSF
                     WRQFAEAFNFKYFFSQKELVLVNVVAAIYFIGFNVYFVHIGNLFISNYGFNAGDAGII
                     QGIGLIIAILFTIPGSRLINRGKSPRVALISILMTIVGLIYLGLIGGYHDPANLFGAK
                     NLPLLLGVIVVGAGYILIMQTLGVWSKVLYPVGSKGQFEGLRIIFFVLIPMVLGSSIS
                     QPIIRHFGIETVVEVLEDGTKVMGYIPTEALFYFASIIMVLALIPLIFLRKEYYKRMR
                     NTKISEAVSTAKEELGVDELYYTTPTPVFDKQK"
BASE COUNT          445 a          267 c          232 g          332 t
ORIGIN      
        1 gtttttgttt atcaaatact ggggtaggag ttgtgtaata tagttcatca acacctaatt
       61 cctctttagc ggtactgact gcttcactaa tttttgtatt acgcatccgt ttataatatt
      121 ctttgcgtaa gaagataagc ggaataagcg ctagtaccat aataatagaa gcgaaataga
      181 aaagtgcttc agtgggaata tagcccatca cttttgtgcc gtcttctaaa acttcaacga
      241 ctgtttcaat cccgaaatgg cgaatgattg gttgactaat acttgaacct aacaccatcg
      301 gtattaacac aaagaagata atgcgtaatc cttcaaattg tcctttacta ccaacagggt
      361 agagcacttt gctccagaca ccgagcgttt gcataattaa aatataacct gcaccaacaa
      421 caataacacc gagtagaagc gggagatttt tggcgccaaa taaattcgct ggatcatgat
      481 aaccaccaat taatcctaaa taaatgaggc caacaatcgt cattaaaatc gaaattaacg
      541 caacgcgcgg actcttgccg cgattaatta agcgactgcc aggtatggta aagagtatcg
      601 caataattaa gccgattcct tgaataatac cagcatcccc agcgttaaac ccatagtttg
      661 aaataaataa attaccaata tggacaaaat aaacattaaa accaataaag taaatagcag
      721 cgacgacatt aacgagtaca agttcttttt gactaaagaa atatttaaag ttaaaagctt
      781 cggcgaattg acgccaaaaa ctaccgcgtt tacttggctc taattttggt gaatctttaa
      841 gtaagaaaag cgaaaggaca ccaattaagc tcacaaagcc gcccatcatt gagaagaagg
      901 caatatagcc aaaagcttca attaaaattc cgcctagtac tgtaccaata atcgtgccga
      961 tgacgggttg aaccgcaagt gcggtaccga ttgccccttt attatcatca ctaattaagt
     1021 cagcggtcca ggcattaaac ccggcatcat ttcccattga tccgaaaaat gacataactg
     1081 tatcggcaag gacaataaca gtcgctagtg cccaaatgtt catcgtaaat tttgtaagta
     1141 gaccagttaa accaaaagca atcgtgaaga taccccaaat gatatagcca atgctgatga
     1201 atggtttcct tttaccagcc cggtcactaa gtgtcccaaa gaagaatgtg gcaaaagttg
     1261 tagcaatcgc cgaaac
//
LOCUS       DAOPCD010000253         1268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_87396_length_1268_cov_2.431987, whole genome shotgun sequence.
ACCESSION   DAOPCD010000253 DAOPCD010000000
VERSION     DAOPCD010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87396_length_1268_cov_2.431987"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /gene="lepA"
                     /locus_tag="PLQ55_05150"
     CDS             complement(<1..1129)
                     /gene="lepA"
                     /locus_tag="PLQ55_05150"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002381601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPK29351.1"
                     /translation="MNYNKDLIRNFSIIAHIDHGKSTLADRLIETTGTLEKREMKAQV
                     LDSLELERERGITIKLNAVALDYLAKDGKTYRLNLIDTPGHVDFTYEVSRSLAACEGA
                     ILVVDATQGVEAQTLANMYLAIDNDLEIIPVINKVDLPSARVEEVREEIYKRIGLDPS
                     KVIGVSAKTGFNIEEVLEAIVKYVPAPSGNVKAPLKALIFDSYYDPYRGVIIMMRVKE
                     GEVRVGDEVLMMATNRKYLVTELGIRTPQEVKRDVLRAGEVGYLAAQIKDIKDISTGD
                     TVTLVNNKAKEALPGYREITPMVYCGLYPVDSKDYGDLKDALEKLSLNDAALKYEPET
                     SVALGFGFRCGFLGLLHMDVIQERLEREHDLNLILTAPSVIY"
BASE COUNT          393 a          274 c          190 g          411 t
ORIGIN      
        1 gataaataac acttggcgct gttaagatta aatttaagtc gtgttcccgt tcaagccgtt
       61 cttgaattac atccatatgt aaaagcccta aaaatccgca gcggaaacca aaacctaagg
      121 caacactagt ttctggctca tattttaagg ctgcgtcatt tagtgaaagt ttctctaaag
      181 cgtctttaag atcaccataa tcctttgaat caactggata gagaccgcaa tataccatcg
      241 gcgtaatttc gcggtaacct ggtaaagctt cctttgcttt attattaact aaagtaacag
      301 tgtcccccgt actaatatct ttgatatctt taatttgcgc agctaagtac cctacttccc
      361 cagcgcgtaa tacatcacgc tttacttctt gcggtgttct aatccctaat tcagtgacta
      421 aatactttct attcgtcgcc atcattaaca cttcatcacc tacacgcact tccccttctt
      481 taacgcgcat cataataatg acaccgcgat aagggtcata ataactatca aaaataagtg
      541 ctttaagtgg tgctttaaca tttccgcttg gtgcaggcac atatttaaca attgcttcaa
      601 gtacttcttc aatattaaaa cctgttttag cgctgacgcc aataacttta gagggatcaa
      661 gaccaatccg tttgtaaatt tcttcacgca cttcttccac tcttgcactt ggtaaatcaa
      721 ctttattaat cactgggata atctctaaat cattatcaat tgctaaatac atattcgcta
      781 gtgtctgtgc ttcaacacct tgggtcgcgt caacaactaa aatcgctcct tcgcacgccg
      841 ctaaagaacg actaacttca taagtaaaat caacatgccc tggcgtatca attaaattta
      901 aacgatatgt tttaccgtct ttcgccaaat aatcaagggc aacagcatta agtttaatcg
      961 taatcccgcg ttctctttct aattctaaag aatcaagcac ttgcgctttc atttcacgct
     1021 tttcaagtgt gcctgttgtc tcaattaagc gatctgcaag ggtcgattta ccatgatcaa
     1081 tatgggcaat aattgagaag ttacggatta aatctttgtt gtaattcata atacttactc
     1141 ttgagatagt attataccaa tttttattaa aattgactaa tgagaaaaga ctaagtacaa
     1201 aacattcata taagtgttag atgacatact tatctttaca aataaagatt aggaaacttt
     1261 taaatttt
//
LOCUS       DAOPCD010000254         1243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_90095_length_1243_cov_2.966330, whole genome shotgun sequence.
ACCESSION   DAOPCD010000254 DAOPCD010000000
VERSION     DAOPCD010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90095_length_1243_cov_2.966330"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PLQ55_05155"
     CDS             <1..475
                     /locus_tag="PLQ55_05155"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPK29352.1"
                     /translation="GVHLLTKPKIEENIKQKQLSGYKAVLDLAQSDVIDLKKETVSDE
                     LAGIGVTNKFSVTIDTTLVGVVYDAEITGYNSGLIFQVGFKGTKYAGFNVISSNETPG
                     YGADYLETVHEQIKGVEIGDPILQDPSKTGKTITANALRKALEACATDYLEGRAG"
     gene            478..1167
                     /gene="rsxE"
                     /locus_tag="PLQ55_05160"
     CDS             478..1167
                     /gene="rsxE"
                     /locus_tag="PLQ55_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HPK29353.1"
                     /translation="MKKRKIFIDGIFANNAAFMLFLGMCPALATTSSFMSALGMGIAV
                     LATLILTNVVISAIRRWVPNEIRIPVLIVIIATTVTIIEMLMQAYMFDLSQTLGVYLS
                     LIVVNCIILGRAEAFAMKNNVLDSLIDAVGTGIGFMLGLLALAFAREFLGTGGIAFTS
                     LLTGQPIWGFQIIPDNYTIGLFQESSGAFIMFGLIVALINFVVAKISENKKKKEAAAK
                     AAAKLAAEGGK"
BASE COUNT          374 a          191 c          271 g          407 t
ORIGIN      
        1 cggagtgcat ttattaacta aaccgaaaat tgaagaaaat attaagcaaa aacaactttc
       61 gggttataag gctgtgcttg acttagcgca aagtgatgtt attgatttaa aaaaagaaac
      121 agtcagtgac gaattagcgg gtattggtgt cactaataaa tttagtgtga caattgatac
      181 cacgctagtt ggtgttgttt atgatgctga aattactggt tataattcag gcttaatttt
      241 ccaagtcggc tttaaaggta caaaatacgc tgggttcaat gttatttcat caaatgaaac
      301 acctggatat ggcgctgatt atttagagac agttcatgag caaattaaag gtgtggaaat
      361 tggtgatcca attttacaag atccaagtaa gacaggtaaa acaataaccg cgaatgcact
      421 gcgtaaagca cttgaagcct gtgctacaga ttacttagaa gggagagcgg gttaattatg
      481 aaaaaacgta aaatctttat cgatggtatt ttcgctaata atgctgcatt tatgctattt
      541 ctaggtatgt gtcctgcctt agcgactaca agttcattta tgagcgcgct cgggatgggg
      601 attgccgtat tagcaacact tatcttaaca aatgtagtga tttctgcgat tagacgctgg
      661 gtccctaatg aaattagaat tcccgtatta attgttatca ttgctacaac cgtaacgatt
      721 attgaaatgc tgatgcaagc ttatatgttt gatctttcac aaacacttgg tgtgtattta
      781 tcactgattg ttgttaactg tattatctta ggtagagccg aagcctttgc aatgaaaaat
      841 aacgtattag atagtcttat tgacgcggtt ggaacgggca ttggctttat gttaggattg
      901 ctagcgttag cgtttgcccg tgaattttta ggaacaggcg ggattgcctt tacttcactt
      961 ttaacagggc aaccaatttg gggtttccaa attattcctg ataactacac aattggttta
     1021 ttccaagaat cttctggtgc ctttattatg tttgggctca ttgttgcctt aattaatttt
     1081 gtggtggcaa agattagtga aaataagaag aagaaggaag ccgcagcgaa agctgccgct
     1141 aaacttgccg cagaaggggg taaataatta tgcaatttct tttaatgggt attggcgctg
     1201 ttttaattaa taatattatt ttaacgcgct tcttaggtat ttg
//
LOCUS       DAOPCD010000255         1237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_90788_length_1237_cov_2.782572, whole genome shotgun sequence.
ACCESSION   DAOPCD010000255 DAOPCD010000000
VERSION     DAOPCD010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90788_length_1237_cov_2.782572"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLQ55_05165"
     CDS             <1..223
                     /locus_tag="PLQ55_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HPK29354.1"
                     /translation="FAKDEEYATLNLNKLHIFISNGINDNVVPYAWGKGSADYFKKRG
                     ADVTFKSYFSGHYVSRDNARDLIDFIVSN"
     gene            complement(242..>1237)
                     /locus_tag="PLQ55_05170"
     CDS             complement(242..>1237)
                     /locus_tag="PLQ55_05170"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HPK29355.1"
                     /translation="ELLGYKLDAIEEVKKVKGPFVMHLSKDDTNHFVLAEFKKGKLSI
                     ADPSGEFYVLSLKNIKKYFISNILVVNCVEKVENPPLVKFKQRYGALIFHSLFLIFLL
                     IGFAFLGHASLDLISYVSFTLAAILKIIEQQVIMKNLQKFDDLYIAPRLVNIKDNFKY
                     KFKVLQAAKTVVFSRPLQLFSAISSTLLITIILVLNNYYFVIISVLLMIVALIEVKRG
                     IKGSAKTFELEVKLSSLAKENNEKRMQLYKEIINDSTHIAAWRVYRSIIIHFSLGVLI
                     FVLMYFTAVYSLNFLIFYFFGFSYYLYELKKLFSLALNTHHYYSAINAINDIPVN"
BASE COUNT          442 a          208 c          174 g          413 t
ORIGIN      
        1 ttttgctaag gatgaagaat atgcgacact aaatcttaac aaactacata tttttatctc
       61 taacggcatt aatgataatg ttgtgcctta cgcttggggt aagggaagcg cggactattt
      121 taagaagcgc ggcgctgatg tgacttttaa gtcttatttt agtggccact atgtttcgcg
      181 tgacaacgcc cgcgatttaa tcgattttat agtaagtaat taatagttaa tcatacatct
      241 tttaattaac cggaatatca ttaatagcgt tgatagcact ataataatga tgagtattca
      301 gcgctaaact aaagagcttt ttaagttcat ataagtaata actgaagcca aagaaataaa
      361 atattaagaa attaagtgaa taaacagcgg taaaatacat taagacaaag attaagaccc
      421 caagactaaa atgaataata atcgaacgat atacccgcca cgcggcaata tgcgttgaat
      481 cattaatgat ttccttatat aactgcattc ttttttcatt attttctttt gctaaactac
      541 ttaatttaac ttctaattca aatgttttag cgctaccttt aatccccctt ttaacttcaa
      601 ttaatgctac aatcatcaaa agcacactga tgattacaaa gtagtaatta ttaagcacta
      661 aaataatcgt tataagtaat gtgctagaga tagcactaaa aagttgtaaa ggtctactaa
      721 aaacaacagt tttagccgct tgcaatactt taaacttata cttaaagtta tctttaatat
      781 ttacgaggcg tggcgctata tataagtcat cgaacttttg taagttcttc attattactt
      841 gttgttcaat aatctttaat atcgctgcta aagtaaatga cacataactt attaaatcta
      901 aacttgcatg acctaggaaa gcaaaaccaa ttaataaaaa gattaaaaac aaagagtgaa
      961 agattaatgc accatatcgt tgtttgaatt tgactaaagg tggattttca accttctcta
     1021 cacaattaac tactaatata ttactaataa aatacttttt aatgtttttt agacttaaaa
     1081 cataaaattc accacttgga tcagcaatgc tgagtttgcc ctttttaaac tctgctagga
     1141 cgaaatgatt cgtatcatct ttacttaaat gcatcacaaa aggtcccttt actttcttta
     1201 cttcttcaat cgcgtcaagc ttataaccaa gtaactc
//
LOCUS       DAOPCD010000256         1231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_91531_length_1231_cov_1.619048, whole genome shotgun sequence.
ACCESSION   DAOPCD010000256 DAOPCD010000000
VERSION     DAOPCD010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91531_length_1231_cov_1.619048"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PLQ55_05175"
     CDS             <1..338
                     /locus_tag="PLQ55_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29356.1"
                     /translation="KGEPNPVAKFINEDINKMVQLIAFMNKHNKVTNLTFKKMTDLIN
                     AFVEHMGGQRELPEGKGFPELFAELNDFALKTLQDAENNWRALFIPIAKEYQDEINKR
                     ERKNPEDLS"
     gene            341..1201
                     /locus_tag="PLQ55_05180"
     CDS             341..1201
                     /locus_tag="PLQ55_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPK29357.1"
                     /translation="MRKTALIFDSTAIIAEEHIKKYDISFVSLNVAIEGVNFRALDIN
                     EEEFKNKFYTYKSVKSGSPSVFEFISAINDKFDKGYKEVVVITLSSGLSSTHAVAACA
                     IENLSPERKAKTFLHDSNNASVGIEPLMYSLLELLDKDPSAAELIKALEVRAPQSRVL
                     FEIDNLRHLYRGGRLNPLKYFIGMALKIKPLIEFENGELKVIGQNRSRRKTMDTILGK
                     IDGFVQKFKNVYVGLFTYATDDNIFKAIHDIIKERWPQIKINFTKKICPVFTTHLGID
                     GYSVAVVSYN"
BASE COUNT          434 a          192 c          235 g          370 t
ORIGIN      
        1 ataaaggcga accaaaccca gtcgctaagt ttattaatga agatatcaat aaaatggttc
       61 aactcattgc gttcatgaat aagcataata aagtgacaaa tttaaccttc aaaaaaatga
      121 cggacttaat taatgctttt gtcgaacata tgggcggaca acgtgaattg ccagaaggta
      181 aaggattccc agaattattc gcggaattaa atgattttgc gcttaagaca ctccaagatg
      241 ctgaaaacaa ctggcgcgcc ttatttattc caattgccaa agaatatcaa gatgaaatta
      301 ataaaagaga aaggaaaaat cccgaagatt tatcgtaaag atgagaaaaa cagcacttat
      361 atttgattcc actgctatta ttgctgaaga acatatcaaa aaatatgata ttagtttcgt
      421 ttcgttaaat gttgcaattg aaggcgtaaa tttccgggct ttggatatta atgaagaaga
      481 atttaaaaat aaattttata cttataaaag cgttaaaagt ggctcaccat ctgtttttga
      541 atttattagt gctatcaacg ataaatttga taagggatat aaagaagttg tcgttatcac
      601 tttgtcgagc gggctaagtt caacccatgc ggttgcggct tgtgccattg aaaatttaag
      661 tcccgaacgc aaagctaaaa catttttaca tgattctaat aatgcctcag ttgggattga
      721 accactgatg tattcacttc ttgaactctt agataaagat ccaagtgccg cggaacttat
      781 taaggccctt gaagttcgtg ctccccaaag tcgcgtatta tttgaaattg ataacttgcg
      841 gcacttatac cgtggtgggc ggctcaatcc tcttaaatat tttattggca tggctttaaa
      901 aattaaaccg cttattgagt ttgagaatgg tgaattaaaa gttattgggc aaaaccgaag
      961 tcgacgcaaa acgatggata caattttagg taagattgat ggctttgtcc aaaaatttaa
     1021 aaatgtgtat gttggtttat ttacatacgc gactgatgat aatatattta aagcaatcca
     1081 tgatataatc aaagaacgat ggccgcaaat taagattaac tttactaaga aaatatgtcc
     1141 ggtctttaca acccaccttg gtattgatgg ctattcagtt gcggttgtaa gctacaatta
     1201 ggaggaaaat atggcgaaaa gaaaaaataa t
//
LOCUS       DAOPCD010000257         1207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_94285_length_1207_cov_3.035590, whole genome shotgun sequence.
ACCESSION   DAOPCD010000257 DAOPCD010000000
VERSION     DAOPCD010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94285_length_1207_cov_3.035590"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..>1207)
                     /locus_tag="PLQ55_05185"
     CDS             complement(41..>1207)
                     /locus_tag="PLQ55_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPK29358.1"
                     /translation="EDSGNLNFISQIALAFIAFSIGAEFSIKSIKKVGKPILVMTSTE
                     VLGAVLVVFFAMFFLPKPQAIMPDGYNPFSKPNVAFGLILASMSAATAPAATLMVIRQ
                     YRAFGPVTRAILPITALDDIFGIVVFGFFISFAQILASTGPLPPVWLMILKPFIEVFG
                     SVALGMIIGLLLSLLANKFDRIRDDLQVLSLIAVFGTIGLTYLINYYLEPHGLSFSQL
                     LSNIMIGSMIANIAKRPEKTFNAINDFATPFYILFFTLAGAGLDLAILGTTPLLLLIA
                     GVYILARGFGKWLGIFVGANVVKAEPTVKKYLGIALLPQGGVSIGLLAIVYTQMAPMY
                     PAISTVIMLSVLVYETMGPVFAKFAISKAGEIGGLDRLEQLSSLDGIELTEEGA"
BASE COUNT          422 a          238 c          239 g          308 t
ORIGIN      
        1 taagataagt catttaaaac aataaataag atttgcataa ttatgctcct tcctcggtta
       61 attcaatacc gtcgaggctt gataattgtt caaggcgatc aagacctcca atttcgccgg
      121 ctttactaat ggcaaatttt gcaaagactg gacccatcgt ttcatacact aatactgata
      181 acattataac agtagaaatg gcaggataca tcggggccat ctgtgtgtag acaattgcca
      241 gtaatccaat tgagacacca ccttgtggta aaagggcaat gcctaagtac tttttcaccg
      301 ttggttcagc tttaaccaca ttagcgccta caaagatacc aagccactta ccaaagccgc
      361 gcgctaggat atagacacca gcgattaata gtaagagcgg ggttgttcct aaaatggcta
      421 aatcaaggcc tgcaccagca agggtgaaga aaagaatata aaatggggta gcaaagtcgt
      481 taatcgcatt aaatgtcttt tcgggacgtt tagcaatatt agcgatcatt gacccaatca
      541 taatgtttga taataattga ctaaatgata aaccgtgtgg ttctaagtaa taattaatca
      601 aatatgttaa tccgattgta ccaaagacag caataagtga taagacttgc aagtcatcac
      661 gaatgcggtc aaacttattc gctagaagcg ataagagtaa accgattatc attccaaggg
      721 caaccgagcc aaacacttca ataaatggct ttaaaatcat gagccacact gggggtaaag
      781 gaccagttga cgctaagatt tgcgcaaaag aaataaagaa cccaaagaca acaataccaa
      841 agatatcatc aagcgcggta attggtaaaa tagcgcgtgt cacaggacca aaagcccgat
      901 attgccgaat aaccataagg gtagcggctg gggcagtcgc cgcactcatt gacgctaaaa
      961 ttaaaccaaa ggccacatta ggtttgctaa atgggttata accatcaggc ataattgctt
     1021 gtggttttgg taaaaagaac attgcaaaga atacgactaa taccgcacct aatacttcag
     1081 ttgaagtcat aactaaaatt ggtttaccga cctttttaat tgatttgata ctaaattccg
     1141 caccaattga aaaagcgata aacgcaaggg cgatttggct tataaagttg agattgccac
     1201 tatcttc
//
LOCUS       DAOPCD010000258         1204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_94646_length_1204_cov_3.792863, whole genome shotgun sequence.
ACCESSION   DAOPCD010000258 DAOPCD010000000
VERSION     DAOPCD010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94646_length_1204_cov_3.792863"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /gene="eno"
                     /locus_tag="PLQ55_05190"
     CDS             <1..1058
                     /gene="eno"
                     /locus_tag="PLQ55_05190"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPK29359.1"
                     /translation="GMDADDQVGIDKVLLELDGTPFKKKLGANALLGVSLAAARAAAD
                     YFGVSLYNYIGGVNAKMLPVPMMNVINGGAHADSSVDFQEYMIMPVGAPSLREALRWG
                     AEVFHNLKKVLKAKGHITAVGDEGGFAPNLSSNEEPLQVIVEAIKKAGYVPGKDFVLA
                     LDVAASEFFDEKTKKYVLKKSGGAVKTSEEMVTWYEELCEKYPIISIEDGLAEGDWDG
                     WKLLTDRLSHKIQIVGDDLFVTNPAILKEGIEKGIANSILIKLNQIGTLTETLDAMQM
                     AERAGYTCVVSHRSGESEDTTIADLAVALNAGQIKTGSMSRTDRIAKYNQLIRIEDDL
                     GERAIYPGLSAFYNLRK"
BASE COUNT          394 a          198 c          260 g          352 t
ORIGIN      
        1 tcggaatgga tgccgatgac caagtgggaa ttgataaagt tttattagaa cttgatggta
       61 ccccatttaa aaagaaatta ggcgctaacg cattacttgg tgttagttta gctgctgctc
      121 gtgccgctgc agattatttc ggtgtaagtt tatataacta cattggtggt gttaatgcca
      181 aaatgttacc agtcccaatg atgaatgtta ttaacggtgg cgcacatgct gatagcagtg
      241 ttgatttcca agaatatatg attatgccag ttggggcacc aagcttacgc gaagcactcc
      301 gctggggcgc tgaagttttc cataacttaa agaaagtttt aaaggccaaa ggtcatatta
      361 ccgcagttgg ggatgaaggc ggctttgcac caaacttaag tagtaacgaa gaacctttac
      421 aagtaatcgt tgaagctatt aaaaaagcag gatacgttcc aggtaaagac tttgttctag
      481 cgcttgacgt cgcggcgagt gaattctttg atgaaaaaac aaagaaatat gtgcttaaga
      541 aatcaggcgg agccgttaaa acaagtgaag aaatggttac atggtacgaa gaattatgtg
      601 aaaagtaccc aattatttcg attgaagacg gtttagcaga aggcgactgg gatggctgga
      661 aacttttaac tgaccgttta agccataaga ttcaaattgt tggtgacgac ttattcgtta
      721 caaacccagc aattcttaaa gaaggtattg aaaaaggcat cgcaaactcc attcttatta
      781 aattaaatca aattggtacc ttaactgaaa cactagacgc tatgcaaatg gcggaacgcg
      841 ctgggtacac ttgtgttgtt agccaccgtt ctggtgaaag tgaagataca actattgccg
      901 acttagcagt cgctttaaac gctggtcaaa ttaaaactgg ttcgatgagc cgtactgacc
      961 ggattgctaa atacaaccaa ttaattcgca ttgaagacga tcttggtgaa agagcaatct
     1021 atcctggttt aagtgctttc tataatttac gcaaataatt gcgtaatatt acttgtcaca
     1081 ttaaaagagg ggaaaccctc tttttttata aatctatatt tttttaatta ttaattatgt
     1141 ataatttaag caggaggttt taactattaa taaatcaagt taaatgataa gagcatagta
     1201 aaaa
//
LOCUS       DAOPCD010000259         1200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_95138_length_1200_cov_3.623581, whole genome shotgun sequence.
ACCESSION   DAOPCD010000259 DAOPCD010000000
VERSION     DAOPCD010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95138_length_1200_cov_3.623581"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /gene="sufB"
                     /locus_tag="PLQ55_05195"
     CDS             <1..1177
                     /gene="sufB"
                     /locus_tag="PLQ55_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HPK29360.1"
                     /translation="FDSYTYFNRVVKDEARSWDDVPDTIKETFEKLGIPEAERKFLAG
                     VATQYDSEVVYASMISEFKEKGVIFSSTDEALKEHPEIFKKYFNTVVKYTDNKYTALN
                     GAVWSGGSFIYVPKGVELDKPLQSYFRLNAAKMGQFERTLIIVDEGASVHYVEGCTAP
                     VYSEDSLHAAVVEIIVHKDAKCRYTTIQNWSNNIINLVTKRADVYENGQMEWIDGNIG
                     SSINMKYPACILRGEGARGTTISIAVAGKGQYQDAGARMIHLAPNTSSTIISKSVVHS
                     GGTANYRGLVRHTKNAPNSQTHIECDTLILDGISKSDTIPTNEIHNNESFIEHEATVS
                     KISEEQLFYLMSRGLSEEDATQMIIMGFIEPFSRELPMEYAVELNRLIKLDMEGSLG"
BASE COUNT          401 a          185 c          255 g          359 t
ORIGIN      
        1 ttttgattct tatacttatt ttaaccgtgt tgtaaaagat gaggcacgct cgtgggacga
       61 tgttccggac acgattaaag aaacttttga aaagttaggt attcctgaag ctgagcgtaa
      121 atttctcgct ggtgtggcga cgcaatatga ttccgaagtt gtttacgcta gtatgattag
      181 tgaatttaaa gaaaagggtg tgattttttc atcaactgat gaggcactta aagaacatcc
      241 tgagatattt aaaaaatatt ttaataccgt tgttaaatat accgataata aatatacagc
      301 cttaaatggg gctgtatgga gcggtggttc atttatttat gtgccaaaag gtgtggaact
      361 agataaacca ctccaaagtt actttagact taatgcggca aagatgggtc aatttgaaag
      421 aacattaatt attgtcgatg aaggggcaag cgttcattat gttgagggtt gtactgctcc
      481 agtttatagt gaagactcat tacacgctgc tgttgttgaa attattgtac ataaagacgc
      541 taaatgtcgc tacactacga ttcaaaactg gtcaaataac attattaatc tcgttacaaa
      601 aagagcagat gtttatgaaa acggacagat ggaatggatt gatgggaata ttggtagctc
      661 tattaatatg aagtatcctg cttgtatctt acgcggtgaa ggagcacgcg gcacaacaat
      721 ttcgattgct gttgcaggga aaggtcaata tcaagatgca ggtgcaagaa tgattcactt
      781 agcaccaaat acatcaagta ccattatttc taaatcagtc gtacatagtg gcggcacggc
      841 aaattatcgc ggtttagtgc gacatacaaa aaatgctcct aattcacaaa cacatattga
      901 atgtgacact ttaatactag acgggatatc aaaaagtgac acaattccta ctaatgaaat
      961 acataacaac gagtcattta ttgaacacga agcaacggta agtaaaatta gtgaagaaca
     1021 gttattttat ttaatgagtc gcggtttaag tgaagaagac gcaacgcaaa tgattattat
     1081 gggctttatt gaaccattct ctcgtgaatt accgatggaa tatgctgtgg aattgaatcg
     1141 tctgattaaa ctagacatgg aaggctcact tggttaatga agtttgatta tgacataatc
//
LOCUS       DAOPCD010000260         1196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_95653_length_1196_cov_2.865907, whole genome shotgun sequence.
ACCESSION   DAOPCD010000260 DAOPCD010000000
VERSION     DAOPCD010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95653_length_1196_cov_2.865907"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /gene="rsmH"
                     /locus_tag="PLQ55_05200"
     CDS             complement(<1..836)
                     /gene="rsmH"
                     /locus_tag="PLQ55_05200"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPK29361.1"
                     /translation="MSTHYPVLLNEVIRGVNVKDDSIIVDLTLGRAGHSSALLFKARN
                     GYLYGFDQDETAIKESREILSKTFQNFTLFHQNFKDFDVALEALGVTGADIILLDLGV
                     SSPQFDVSDRGFSYRYEGPLDMRMDRTNNTLTAAIIVNTYSEQKLKEIFYRYAEHRYS
                     ARIAREIVNARNEQEIRTTTELVEIIKRALPSKELRKKGHPAKTIFQALRIEVNNELG
                     VLEKVLERAPHFLNEGGRLAVISFHSLEDRLVKNVFKKLATLSDEGLHPYTLPDKNRV
                     AP"
     gene            complement(836..>1196)
                     /locus_tag="PLQ55_05205"
     CDS             complement(836..>1196)
                     /locus_tag="PLQ55_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29362.1"
                     /translation="LKDLKTLYILQGFDGAISVYPEEAYHQELSFLEAQNFKNADARA
                     YIRTIYASIEELEVDAAGRITLPVRIQKKYDIGQKVIVIGVGDHLEIWDEERYLSYER
                     DACGNLEDLAAKLQEKA"
BASE COUNT          288 a          248 c          247 g          413 t
ORIGIN      
        1 aagggtgcta ctctattttt atcaggtagt gtgtaaggat gcagtccttc gtcactcagt
       61 gtcgctaact ttttaaagac attttttact aagcggtctt caagactatg gaagcttatt
      121 actgcgagtc tccccccttc atttaagaag tgtggagcgc gttccaagac tttttcaagt
      181 actcctaatt cgttatttac ttcaatgcga agtgcttgga agatcgtttt agcgggatgg
      241 cctttcttgc gtaattcttt gcttggtaag gctcttttta taatttcgac taactcggtt
      301 gttgttctta tttcttgctc gtttctcgca tttacgattt cgcgagcaat gcgggcactg
      361 tagcgatgtt ccgcgtagcg gtagaatatt tcttttagtt tttgttctga ataagtgttg
      421 acaataatcg ctgctgttaa ggtattgttc gtccggtcca ttcgcatatc aagtggacct
      481 tcatagcggt aactaaagcc gcggtcactt acatcgaatt gcggactact gacacctaag
      541 tcaagtaaga tgatgtcagc gcctgtgact ccaagtgctt ctagcgcaac atcgaagtct
      601 ttgaagtttt gatggaagag cgtgaagttt tggaatgttt tactgagtat ttcccgcgac
      661 tcttttatcg cagtttcatc ttggtcgaag ccgtaaagat agccgttgcg ggctttgaag
      721 agtaaggcac tgctatgccc cgctcgtcct aaggttaagt caacaatgat actatcatct
      781 ttgacattta cgcctcttat tacttcatta agtaatactg gataatgcgt actcattagg
      841 ctttttcctg taacttagca gctaggtctt ctaagttacc gcacgcatct ctttcgtaac
      901 ttaagtagcg ttcttcatcc cagatttcaa gatgatcacc gacgccaatt acaattactt
      961 tttgcccaat gtcatatttc ttttgaatac gaactggtaa ggtaatacgg cctgctgcat
     1021 ctacttctaa ctcttcaata cttgcgtaaa ttgtacgaat atacgctcta gcgtcagcat
     1081 ttttgaagtt ttgtgcttca aggaaagaaa gttcttggtg gtaagcttct tcgggataca
     1141 cacttattgc cccatcaaag ccttgtaaga tgtaaagcgt ttttaagtct ttaagt
//
LOCUS       DAOPCD010000261         1186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_96925_length_1186_cov_3.573828, whole genome shotgun sequence.
ACCESSION   DAOPCD010000261 DAOPCD010000000
VERSION     DAOPCD010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96925_length_1186_cov_3.573828"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..>1186
                     /locus_tag="PLQ55_05210"
     CDS             47..>1186
                     /locus_tag="PLQ55_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29363.1"
                     /translation="MKKLRYLLILLSASLVLSGCKGGKVSEEETLPELTNMTVDGVIN
                     EEAYNVIPGRTAGVKAGVLVQYAVGREGVYFGLTVTDTKHQVSAPSGIVASDYVGLAV
                     DALAVPKSDDGVSESTKLFRVDTMGRYTYTTGNEYGAWVDVDSGVYEGVISGEEIPTI
                     AVNIVKDQYYVVEIFFTWEHLGTTEEAAKDANCLMYYIEHRDMGVDIHADANILAPAM
                     YNRLVYLGNRKGSNLPADPPEITIDGVLDETLWDTAFVTNSGNFKERVPDETAGDYRT
                     LAIWGEKGIYLGVEVEDPHIEAPNGTGAAYKNAGMEMRIHVYNKDGLPLNSLKWLFDV
                     WGPQWHETGAGGLASSFAPYAEYKFVISGTIDNNEDTDEGWSFEIY"
BASE COUNT          354 a          192 c          283 g          357 t
ORIGIN      
        1 tccagtgatc ccctcgctgg aagaactttc agtaaaggag ttttaaatga aaaaactacg
       61 ttatttactt atattgctct ctgctagtct tgtattaagt ggttgtaaag gtggtaaagt
      121 ttctgaagaa gaaacattac ccgaattaac aaatatgaca gtagatggcg taataaatga
      181 agaagcttat aatgtcattc ctgggcgcac agcgggtgtt aaagcaggag tattagtgca
      241 gtatgcagtt ggtagagagg gtgtctattt tggcttaact gtgacagaca caaaacatca
      301 agtttctgca ccaagtggga ttgttgctag tgactatgtt ggtcttgcgg ttgatgccct
      361 tgctgtccca aagagtgatg atggtgtgtc tgaatcaaca aagttgttcc gcgtcgacac
      421 gatgggccgt tatacttaca ctacaggtaa tgaatatggt gcatgggttg atgttgatag
      481 cggagtttat gaaggcgtta taagtggaga agaaattccg acaattgctg ttaatatcgt
      541 taaagatcag tattatgttg tggaaatatt ctttacctgg gaacatttag gcacaactga
      601 agaagcagca aaagacgcta attgtttaat gtattatatt gaacaccgtg atatgggtgt
      661 tgatattcac gctgatgcaa acattcttgc gccggcaatg tacaatcgcc ttgtttatct
      721 tggtaatcgt aaaggtagta accttccagc agatcctcca gaaataacaa ttgatggcgt
      781 cctcgatgaa acattatggg acacagcttt tgtcactaac tctggcaatt ttaaggaacg
      841 cgttcctgat gaaaccgctg gtgattatcg cacattagca atttggggtg aaaaaggtat
      901 ttatcttggt gttgaagttg aagatccaca tattgaagca ccaaatggta ctggagccgc
      961 ttataaaaat gctggtatgg aaatgcgtat acatgtttat aataaggacg gtttaccact
     1021 taattcactt aaatggctct ttgatgtatg gggaccacaa tggcacgaaa ctggcgctgg
     1081 gggtctagca tcatcttttg cgccatatgc tgaatataaa tttgtgatta gcggcacaat
     1141 tgataataac gaagatactg atgaaggatg gagttttgaa atttac
//
LOCUS       DAOPCD010000262         1185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_97067_length_1185_cov_3.864602, whole genome shotgun sequence.
ACCESSION   DAOPCD010000262 DAOPCD010000000
VERSION     DAOPCD010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97067_length_1185_cov_3.864602"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PLQ55_05215"
     CDS             <1..761
                     /locus_tag="PLQ55_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPK29364.1"
                     /translation="PLMWGYFTWMRPYARRPDKYPPELRMAKGQKLARRVLRILKVDV
                     EIRNMPDFKRDEIYYIVSNHTSVLDLVVLLAYLPIPVSFLSKVENKKIPFVRTLMRVY
                     KGLYLERNNLRQEIKVMQMTRDSLANKEVSWLIFPEGTRSKDYHGNLSDYKAGAFKAP
                     LATNTTIVPVVMWGNQMVLPLRTRARRYKVIVEFLPLLKPEGTTLELANKIYEASNAT
                     ASKLREEYGSVQKINKYGRKIMTLQLSENIKTSK"
     gene            873..>1185
                     /gene="deoA"
                     /locus_tag="PLQ55_05220"
     CDS             873..>1185
                     /gene="deoA"
                     /locus_tag="PLQ55_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418497.1"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HPK29365.1"
                     /translation="MRMYDIILKKRDGFALTEEEIKFFIKGVTDGTIPDYQTSSLLMA
                     IVWRGMNKEETAALTLAMMHSGDVIDLSAIKGVKVDKHSTGGVGDKTSLVLGPLVASF
                     GV"
BASE COUNT          383 a          200 c          256 g          346 t
ORIGIN      
        1 tccccttaat gtggggttat tttacgtgga tgcgccccta cgcacggcgt cctgataagt
       61 accctcccga attgcggatg gcaaaaggac aaaaacttgc acggcgtgtg ctacgcattt
      121 taaaagttga tgtggagatt cgtaatatgc cagactttaa gcgtgacgaa atttattaca
      181 tcgttagcaa tcacacgagt gtgttagatc ttgtcgtctt acttgcgtat cttccaatcc
      241 ccgtgtcctt tttatcaaaa gtcgaaaata agaaaattcc gtttgtgcgg acactgatgc
      301 gggtgtacaa aggtttatat cttgaacgca ataacttacg ccaagaaatt aaagtaatgc
      361 aaatgactcg tgactctctt gctaataaag aagtaagttg gcttattttc cccgaaggaa
      421 cgcgcagtaa agattatcac ggtaatcttt cagattataa agccggtgct tttaaagctc
      481 ccttagcgac aaatacaacg attgtccccg ttgtgatgtg gggtaaccaa atggtcttac
      541 cactaagaac aagagcgcgg cgttataaag taattgtcga atttttacca cttttaaaac
      601 ctgaaggcac aacgcttgaa ctcgctaata agatatatga agcaagtaat gcgaccgcta
      661 gtaaacttcg tgaagaatac ggaagtgtcc aaaaaattaa taaatacggg cgtaaaatta
      721 tgacgctgca acttagtgaa aatattaaaa catctaaata atttgcttaa tttaatgaaa
      781 aaaatgacga taaaaagtat agtttactct atctgagtag agtaagcgct ttttagtatg
      841 gtaaaatagt tttaataaag gagatattaa ctatgcggat gtatgacatt attttgaaaa
      901 aacgcgatgg tttcgcactt actgaagaag aaattaaatt ctttattaaa ggagtaactg
      961 acgggactat tcctgattat caaacgagta gtttactaat ggcgattgtg tggcgcggaa
     1021 tgaataaaga agaaaccgcc gccttaacac tagcgatgat gcatagtggt gatgttattg
     1081 atttaagtgc cattaaaggt gttaaagttg ataaacatag cacgggtggt gttggtgata
     1141 aaacaagctt agtgcttggt ccgcttgtcg ctagttttgg ggtta
//
LOCUS       DAOPCD010000263         1184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_97202_length_1184_cov_3.782108, whole genome shotgun sequence.
ACCESSION   DAOPCD010000263 DAOPCD010000000
VERSION     DAOPCD010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97202_length_1184_cov_3.782108"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLQ55_05225"
     CDS             <1..555
                     /locus_tag="PLQ55_05225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242913.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPK29366.1"
                     /translation="YMYTDLASLYERAGRVKGLKGSITQIPILTMPEDDKTHPIPDLT
                     GYITEGQIILSRPLSMKGLTPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFSAYA
                     RGKDAKELSLILGDSALSEIDQKYAAFADAFEREYVSQGFDTNRSIKETLDLGWKLLG
                     MLPRQELRRVDPKLVDKYYKESKE"
     gene            559..>1184
                     /locus_tag="PLQ55_05230"
     CDS             559..>1184
                     /locus_tag="PLQ55_05230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPK29367.1"
                     /translation="MSTAQINPTRMELQRLRDKLATTTRGHKLLKDKQDELIHEFIPL
                     LPEYRALREKVESALSATLIRYQFACVKMSKEAINDHINDSVTKHELSAKFDRVMGVV
                     IPNLETKASHTEVNYDLLSTTSAFDDLITAIQDLFALLIELANLENTIKILVAEIEKS
                     KRRVNAIENIVIKEIEEQIRVIRMKLTDLERSNTIRMMKSKEIIMEKK"
BASE COUNT          412 a          210 c          239 g          323 t
ORIGIN      
        1 tatatgtaca cagacttagc ctcgctctat gaacgcgcgg gtcgcgtaaa gggacttaaa
       61 ggttccatta cccaaattcc gattttaacg atgcctgaag atgataaaac gcacccaatt
      121 ccagacttaa cgggttatat taccgaagga caaattattt tgtcccgtcc cctttcaatg
      181 aaaggcttaa cgccaccgat tgatgtttta ccttcacttt cacggcttaa agataaaggg
      241 attggtaaag gtaaaacgcg tgaagatcac gctgatacaa tgaatcagct ctttagtgcg
      301 tatgcgcgcg gtaaagatgc aaaagaatta tcacttattt taggtgatag tgcactttct
      361 gaaattgacc aaaaatacgc ggcttttgcc gatgcttttg aaagagaata cgtttcacaa
      421 ggctttgaca ctaaccgtag tattaaagaa acacttgatc ttggatggaa acttttagga
      481 atgttaccac ggcaagaatt acggcgtgtt gatccaaaac tcgttgataa atattataag
      541 gaaagtaaag agtaattaat gagcactgcg caaattaatc caacccggat ggagttacaa
      601 cggttacggg ataaactagc gaccacaacg cgtggtcaca agcttttaaa agacaaacaa
      661 gatgaattaa ttcatgagtt tattccgctg ctacctgagt accgcgctct gcgggaaaag
      721 gttgaaagtg cattaagtgc aactttaatt cgctatcaat ttgcgtgtgt caaaatgagt
      781 aaagaagcaa ttaatgacca tattaatgac agtgtaacga aacatgaatt aagtgcaaag
      841 tttgatcgcg taatgggtgt agtcattcct aacttagaaa caaaagctag tcacactgaa
      901 gttaattatg atttactatc gacaactagt gcctttgatg atttaatcac agcgattcaa
      961 gatttatttg cgttactcat cgaacttgct aacttagaaa ataccattaa aatcttagtg
     1021 gcggaaattg agaaatcaaa acgccgtgtt aatgcgattg aaaatattgt cattaaagaa
     1081 attgaagaac aaattcgtgt gatacgcatg aaactaacag acttagaacg cagcaataca
     1141 attcggatga tgaagagtaa agaaatcatc atggaaaaga aatt
//
LOCUS       DAOPCD010000264         1162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_100147_length_1162_cov_3.262873, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000264 DAOPCD010000000
VERSION     DAOPCD010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100147_length_1162_cov_3.262873"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..>1162
                     /locus_tag="PLQ55_05235"
     CDS             174..>1162
                     /locus_tag="PLQ55_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPK29368.1"
                     /translation="MSREKLANIIFPEITTTIADLEERYPHRSLKDGAEVTRFAPSPT
                     GFLHTGSLFAALVSRTVAHRSGGVFFVRLEDTDTKREIAGAGENLLNEMKIFKVYPDE
                     GYLGDSELGDYGPYKQSERALIYKTVIKQMIKDDLAYPCFCTTDDLALLRATQEASKV
                     VSGYYGHYATCSKLSADEAIAKIEAGVPYVIRFRSKGNHENRVSFDDEVKGTIEIAEN
                     DQHIVILKSDGLPTYHFAHVCDDHFMRTTLVSRGEEWISSVPLHLEMFAAVKFNQPRY
                     AHLPTIDKLDDGNKRKLSKRRDPEAAVTYFLELGYPVDAVLTYLMSIANSNFE"
BASE COUNT          385 a          187 c          228 g          362 t
ORIGIN      
        1 gaacccgcgt atctaccttg gcaaggtagt gttctaccat tgaactacac ctgcgtgctt
       61 gattatatta tcactcttat gaaatttatt caattaatgt ccttataaat tagtaaaatt
      121 gatgaaaaat atacatagta tggtatattt atatgtgtta aaagaggtta attatgagca
      181 gagaaaaatt agcgaatatt atatttccag aaattactac aacaattgcc gatttagaag
      241 aaagatatcc gcatcgtagt ttaaaagatg gtgccgaagt tacacgattt gccccctcac
      301 ctacaggatt tttacacact ggtagtttat ttgcggcttt agtttctcgc actgttgcgc
      361 accgaagtgg tggggtgttc tttgtaagac ttgaagacac tgatacaaaa cgtgaaattg
      421 caggcgctgg tgaaaattta cttaatgaaa tgaaaatatt taaagtatac cctgatgaag
      481 gctacttagg tgatagtgaa ttaggagact acggcccata taaacaaagt gaacgtgctc
      541 ttatttataa aacagtaatt aaacaaatga ttaaggacga tttagcttac ccttgttttt
      601 gcactactga tgatttagcg cttttacgtg caacacaaga agctagtaaa gtagtctctg
      661 gttattacgg acattacgca acatgtagta aattaagtgc agatgaagcg atcgctaaaa
      721 ttgaagcagg cgttccttat gttattcgtt ttagaagcaa aggaaatcat gaaaatcgtg
      781 tttcatttga cgatgaagta aaaggtacga ttgaaattgc ggaaaacgat caacatatcg
      841 ttattttaaa gagtgacgga ttaccgacat atcactttgc ccatgtttgt gatgatcact
      901 ttatgcgtac aacactcgtc tcacgtggtg aagaatggat tagtagtgtc ccattacatt
      961 tagaaatgtt tgcagcagtt aaatttaatc aaccgcgtta tgcacactta ccaacaatcg
     1021 ataaattaga tgatggtaat aaacggaaat taagtaaaag gcgtgatcca gaagccgctg
     1081 ttacatattt cttagaatta ggatatccag ttgacgctgt tcttacttat ttgatgagca
     1141 tcgctaattc aaactttgaa ga
//
LOCUS       DAOPCD010000265         1158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_100664_length_1158_cov_3.361741, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000265 DAOPCD010000000
VERSION     DAOPCD010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100664_length_1158_cov_3.361741"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PLQ55_05240"
     CDS             complement(<1..893)
                     /locus_tag="PLQ55_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPK29369.1"
                     /translation="MSKKFKFNKGLIPKLIVIGIFLALVIIAIISDKVLTGTAFGDFV
                     NNTIGKFFNIIDILKNHWITIAESIVIIVFFWLLNKLFEYILLPLGKKRNKHSTWWVL
                     FRSIFKYLSSAVAIFLVLSAWGVDTPTLLIGAGIVGLAISFGAQSLIEDVIAGLFIIF
                     EKQFKIGDVIQVGETRGYVIDIGIRTTSIEDVYGDILIVNNSDLRMILNTSANLSPAI
                     SEIYISYDQPLENVENIVKKALPTLKERIPDIIEGPSYRGVQKLDSNGVLIRVYAKVE
                     DLKKFQVVRDMNRELKIVFEK"
     gene            complement(908..>1158)
                     /locus_tag="PLQ55_05245"
     CDS             complement(908..>1158)
                     /locus_tag="PLQ55_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29370.1"
                     /translation="DVVDEGSIISGAYAECFARDEYGELVPMYINSFENVSATAGESN
                     QYECMVFYTEQNIVEIRVYINKSYNYQYENELIYIVTI"
BASE COUNT          374 a          251 c          153 g          380 t
ORIGIN      
        1 tttttctcga acacaatttt taattcacga ttcatatcac gcactacttg gaacttcttt
       61 aagtcttcaa ctttcgcata cacacggatt aaaaccccat tactatcaag cttttgcaca
      121 ccgcggtaac ttggtccttc aataatgtca ggaattcttt ctttaagtgt tggtaaagct
      181 ttctttacta tattttcaac attttctaaa ggttgatcgt aacttatata aatttcacta
      241 attgcgggtg ataagtttgc gcttgtattt aaaatcatcc gtaagtcact gttattcaca
      301 attaaaatat caccatagac atcttcaatt gaagttgttc taattccgat atcaataacg
      361 tatcctcttg tttctccaac ctgaattacg tcaccgatct taaactgttt ttcaaagatg
      421 ataaataacc cagcaatcac gtcttcaatt aagctctgcg caccaaaact aatcgctaac
      481 ccgacaattc cggccccgat taagagtgtt ggggtatcaa caccccacgc acttaagaca
      541 aggaaaatcg ctactgcact actaagatac ttaaagatac tgcgaaataa tacccaccat
      601 gttgagtgtt tattacgttt ctttcctagt ggcaataaaa tatactcaaa gagtttattt
      661 aagagccaga aaaacacaat aatcacaata ctttccgcaa tggtaatcca gtgattcttc
      721 aagatgtcaa taatgttaaa gaacttccca atcgtgttat taacaaagtc accaaacgct
      781 gtacctgtta gaaccttatc actaataatt gcaataatca ctagcgctaa aaagatgcca
      841 ataacaatta gcttcgggat tagtccttta ttaaatttaa acttcttact cataatcgtt
      901 ccctccttta aatggtaact atataaatta attcgttttc atattgatag ttatatgact
      961 tatttatata cactctaatt tcaacaatat tttgttcagt gtaaaatacc atacattcgt
     1021 attgattact ctcacccgca gtagctgaca cgttctcgaa actattaatg tacattggca
     1081 ctaattcgcc gtattcgtcg cgtgcaaagc attcagcata agcgccgcta atgatgcttc
     1141 cttcatcaac aacatcaa
//
LOCUS       DAOPCD010000266         1157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_100810_length_1157_cov_3.022686, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000266 DAOPCD010000000
VERSION     DAOPCD010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100810_length_1157_cov_3.022686"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1157
                     /locus_tag="PLQ55_05250"
     CDS             <1..>1157
                     /locus_tag="PLQ55_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK29371.1"
                     /translation="LVVWSGFTNGYGIYVMREIIDKSILDFDINGLNKSLIKLAILFG
                     SGALSALIYNPMMVKYSQTVIADIRNELFAHSQKLPLSYFDRTPRGEIMNYFTNDVDT
                     LNQALNTGFTTMIYSVVTGIGTIVFLFLINFYLSLIVVFFIVVMFFMVLRNSQKMRVG
                     FASVQKDTAHLNSYIEEMMLGLKVSAMFSHQKEDMKEFKRRNDALTKSGIYANTLVLR
                     KTPMIVSLSYFNYAISAIAGALFVLNGWLSLGALGSYLIYVRQSSNPFNNFMTELNAL
                     YNGLSGAERIFAFLDLEQEIDEGKVRLVSAVEIDGNIVETEHPTNSLAWKIPTANGEF
                     TYEIFKGRVDFTNVVFGYKQDERVLNNISLHAEKGEKVALVGSTGAGKTTI"
BASE COUNT          353 a          176 c          237 g          391 t
ORIGIN      
        1 tccttgttgt gtggagcggg tttactaatg gttacggtat ttatgtaatg cgtgaaatca
       61 tcgataaaag catattagac tttgatataa atggtttaaa taagtcactt attaaattag
      121 cgatcctttt cggttcaggt gctttaagtg cacttattta taacccgatg atggttaaat
      181 attcacaaac tgttatcgcc gacattcgca atgaactttt cgctcatagt caaaaattac
      241 cgctatctta ttttgacagg acaccgcgcg gtgaaattat gaactatttc actaatgatg
      301 ttgacacact taatcaagca cttaatactg ggtttacaac gatgatttat tcagtagtaa
      361 caggcattgg cacaattgta tttcttttcc ttattaattt ttatttgtca ctgattgttg
      421 tctttttcat cgttgtaatg ttttttatgg tactgcgtaa tagtcaaaaa atgcgggttg
      481 gttttgctag tgttcaaaaa gatactgcgc atttaaatag ttatattgaa gaaatgatgt
      541 tagggcttaa agtatcagca atgttttcac accaaaaaga agacatgaaa gaatttaagc
      601 ggcgcaatga tgcattaacg aaaagcggaa tatacgcgaa tactttagtg ttacgaaaga
      661 caccaatgat tgttagtttg tcgtatttta attatgcgat tagcgcgatt gctggtgcgc
      721 tttttgtatt aaatggctgg ctaagtttag gagcactagg atcatactta atttatgtcc
      781 gccaaagttc aaatccgttt aataacttta tgactgaatt aaatgctctc tataacgggc
      841 tttcaggggc agaacggatt tttgcttttc ttgacttaga acaagaaata gatgaaggta
      901 aagtccggct tgtcagcgct gttgaaattg atggtaatat cgttgaaact gaacacccga
      961 ctaatagttt agcgtggaag attccgacgg ctaacgggga attcacctat gaaatattta
     1021 aagggcgagt cgattttact aatgttgttt ttggttataa gcaagatgaa cgcgtcttaa
     1081 ataatattag tttacatgcc gaaaaaggtg aaaaagttgc gcttgttggt tcaactggtg
     1141 ccggtaaaac aacaatt
//
LOCUS       DAOPCD010000267         1152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_101524_length_1152_cov_2.621696, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000267 DAOPCD010000000
VERSION     DAOPCD010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101524_length_1152_cov_2.621696"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PLQ55_05255"
     CDS             <1..715
                     /locus_tag="PLQ55_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK29372.1"
                     /translation="ILTMELKEQDPDYIAVSDEDIKLLEKVVKDVNKKSTVTKIDVTG
                     LYIEHLLESPNSKTRYTAYTLLRLLLDEVAGIPLKVLYLDTDVLANDDISLLYNTDIS
                     NYELAGVKDRYGRFFMGPRYLNAGVLLLNLKLIKETNLFAKARHYLNKKKVFLSDQTA
                     INKYVRKKLILKRVYNEQKKTKPDTVIRHFSMQFRLFPKFHFVNIKQWDIERLHSVYK
                     CHAFDDILYKYQQIINKEI"
     gene            717..>1152
                     /locus_tag="PLQ55_05260"
     CDS             717..>1152
                     /locus_tag="PLQ55_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK29373.1"
                     /translation="MKKHEIPVFFACDDNYIPFLGTALHTMIKNASKDYHYKAIVLNA
                     GIKEENKNRIHALENENLTIEFFNISELITKHYENLALRLRDYYSVAIYYRMFIPTLF
                     PQYEKAIYLDADLILVGDISELYNTNIEGELVGAINDRLVLDV"
BASE COUNT          404 a          186 c          203 g          359 t
ORIGIN      
        1 tattttaaca atggagttaa aagaacaaga tccagattac attgctgtta gtgatgaaga
       61 tattaaatta ttagaaaaag tagtgaaaga tgttaataaa aagtctactg tcacgaagat
      121 tgacgtaaca ggactttata ttgagcatct ccttgaaagt ccgaatagta agacgcgcta
      181 cactgcttat acattgttac gtttattatt agatgaagta gcgggtatcc ctcttaaagt
      241 gctttattta gacactgatg ttttagcaaa cgatgatata agccttcttt ataacaccga
      301 tattagtaat tacgaactcg cgggtgttaa agatcgttat ggtcgtttct ttatggggcc
      361 acgctattta aacgctggtg tcttattact taacttgaaa cttattaaag aaacaaactt
      421 atttgcgaag gcaagacatt acttaaataa gaagaaagtg tttttaagtg atcaaaccgc
      481 gattaataaa tatgttcgta aaaagcttat tttaaaaaga gtgtataacg aacaaaaaaa
      541 gacaaagccc gacactgtta tccggcactt cagtatgcag tttcggctct ttccaaaatt
      601 ccatttcgtt aatataaaac aatgggatat tgaaagatta cattccgttt ataaatgtca
      661 cgcctttgat gacatcttat ataaatatca acaaattata aataaggaga tttaatatga
      721 aaaaacatga aataccagtc ttttttgcct gtgatgacaa ctacattccg tttctaggca
      781 ctgctttaca tacaatgatt aaaaatgcga gtaaagatta tcattataaa gcgattgtct
      841 taaacgcggg catcaaggaa gaaaacaaaa accgcattca cgcgttagaa aatgaaaact
      901 taaccattga attctttaac attagtgaac taattacaaa gcattatgaa aacttagcgc
      961 tcaggcttcg tgattactat agtgtcgcta tttattatcg gatgtttatt ccgactttat
     1021 ttccgcagta tgaaaaagcc atctacttag atgcagactt aatcttagtg ggcgacatta
     1081 gtgaactata taacacaaac attgaaggcg agcttgtcgg ggcgattaat gaccgtctcg
     1141 tccttgatgt gc
//
LOCUS       DAOPCD010000268         1152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_101562_length_1152_cov_2.186873, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000268 DAOPCD010000000
VERSION     DAOPCD010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101562_length_1152_cov_2.186873"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1152)
                     /locus_tag="PLQ55_05265"
     CDS             complement(<1..>1152)
                     /locus_tag="PLQ55_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPK29374.1"
                     /translation="TYTASQGLLLMVPNIYKWVGELLPAVLHVSARSIAARSLSIFGD
                     HQDIYAVRQTGVTMIASHSVQEVADLAHAAHLTAIGSSAPVLHFFDGFRTSHEIQKVE
                     VIDEAEWKKLLPEDKVAEFRARALNPHANPVTRGGAENDDICFQGREAQNAHYARVLG
                     VAEDYFAKVSKLTGRHYAPFVYRGAKDATKVIIAMGSVTEAISEVVDDLTKKGEKIGL
                     VKVHLYRPFSAKHLVSVLPETVKKIAVLDRTKELGALGEPLYIDVLTALKQEGRKVDV
                     IVGGRYGISSRDTQPKHIKGVYDFLDKKPWHSFTVGINDDVTKLSVPYDDNYHIDNGH
                     TECLFYGLGSDGTVSANKSSVAIIGEHTDKYAQAYFAYDSRKAGGPTRS"
BASE COUNT          337 a          258 c          222 g          335 t
ORIGIN      
        1 gactacgagt tggtccgcct gctttccgcg agtcataagc aaagtaagct tgcgcatatt
       61 tatcagtatg ttcaccaata atagcaacac ttgatttatt agcgctgaca gtgccatcac
      121 tccctaaacc gtagaataag cattcggtat gaccattatc aatatgatag ttatcatcat
      181 aaggaacact aagtttagtc acatcatcat taatgccaac ggtaaaggag tgccacggtt
      241 tcttatctaa gaagtcataa acacctttaa tgtgtttggg ttgggtatca cggctactaa
      301 taccgtaacg gccaccaacg atgacatcaa ctttgcgacc ttcttgctta agggccgtta
      361 aaacatcaat ataaagaggt tccccaagag cacctaattc tttagtgcga tctaaaactg
      421 caattttttt aactgtttct ggtaaaacac taactaaatg tttagcgcta aacgggcgat
      481 ataagtgaac tttaacaagt ccaatcttct cacctttttt cgttaagtca tctactactt
      541 cactaattgc ttcggtaaca ctacccatcg caatgataac tttggttgca tctttagcgc
      601 cgcgataaac aaagggggcg taatgacgac ctgttaattt acttacttta gcaaagtaat
      661 cttccgcaac acctaagacg cgagcgtaat gcgcgttttg ggcttcgcga ccttggaaac
      721 aaatatcatc gttttccgct ccaccacggg taacgggatt cgcgtgcggg ttaagcgcac
      781 gagcgcgaaa ttccgctact ttatcttctg gtaaaagttt tttccattcg gcttcatcaa
      841 tgacttcaac tttttgaatt tcatgtgatg ttctaaaacc atcaaagaag tgtaagactg
      901 gagcacttga tccaattgct gttaagtgtg ctgcgtgcgc taaatcagcg acttcttgga
      961 cactgtgact tgcaatcatc gtcacaccag tttgccgtac agcataaata tcttgatgat
     1021 caccaaaaat ggataaggaa cgcgcggcaa tactacgggc actaacatgc agtactgctg
     1081 gtaaaagttc accaacccat ttgtaaatat ttggcaccat taaaagtaaa ccttgtgacg
     1141 cggtatatgt tg
//
LOCUS       DAOPCD010000269         1146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_102368_length_1146_cov_2.367553, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000269 DAOPCD010000000
VERSION     DAOPCD010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102368_length_1146_cov_2.367553"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="PLQ55_05270"
     CDS             complement(<1..1133)
                     /locus_tag="PLQ55_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK29375.1"
                     /translation="MLFYYDPENPTINYLYRPLWEKLIKAGHEINIITPNPTRGIPLE
                     EQEKYNKSSVEVNGKITIYRIKCYTYSPKRFSKFKLLQRYISVARRLAKKLRKVKSDA
                     VFVSSNPPLLYSYLVTKYCARKKIPVVYNVQDIYPDNVFSKKHLAYKLFNPFQVKSLK
                     RATEVITISETMRRTLLAKGDFNDKISVIYNFDISEVAKAPTLKKYTFFDPLQFNVVY
                     AGNIGYVQDIDVILSAAKLLVHEPRIHFHIVGEGSQAARIAARIHDENIVNATYYPPH
                     SVEDSAQLYKNADVNIISILPGIIKTALPLKTASCLAAQKPIIFIGLETFYHHEEWVN
                     TIPELYPVNYRNYHKVAEIIEKMANKKTKPKLNYSPLVIFDKK"
BASE COUNT          355 a          202 c          206 g          383 t
ORIGIN      
        1 ttctttttat caaatattac aagcggacta taatttaatt taggctttgt ttttttattc
       61 gccatctttt caataatctc ggcaacttta tgataattac ggtaattaac gggataaagt
      121 tcggggatag tattaaccca ttcttcatgg tgatagaaag tttcaagccc aatgaagata
      181 attggttttt gggccgctaa acaactcgcc gttttaaggg gtaaggcggt tttaataatg
      241 cccggtaaga ttgagataat gttaacatca gcgtttttat aaagttgcgc ggagtcttca
      301 acactatgcg gcggataata cgtcgcgttt acaatatttt catcatgaat ccgcgcggca
      361 atacgagcgg cttgactccc ttcaccaaca atatggaaat ggatacgtgg ttcatggact
      421 aaaagtttag ccgcacttaa gattacatca atatcttgga catagccaat attaccagcg
      481 tagacgacat taaactgtaa gggatcaaag aacgtgtatt tttttaaggt cggggctttc
      541 gctacttcac taatatcaaa attataaata acactgattt tgtcgttgaa atcgcctttt
      601 gctaataaag tgcgccgcat tgtttcacta atagtgataa cttcagttgc ccgttttaaa
      661 cttttaactt ggaagggatt aaataattta taagcaagat gtttctttga aaaaacatta
      721 tctggataaa tatcttgcac attgtaaaca actggaattt tcttacgggc gcaatactta
      781 gtgacaagat aactataaag taggggcggg ttacttgaca caaacacagc gtcactttta
      841 actttccgta atttcttcgc taaacgacgt gcgacactaa tataacgttg aagcaattta
      901 aacttactaa agcgtttagg ggaatatgta taacacttaa tccgataaat tgtaattttt
      961 ccgttgactt caacactact tttattatat ttttcttgtt cttctaaagg tataccccgg
     1021 gttggatttg gcgtaataat attaatctca tgccccgcct taattaactt ctcccaaagt
     1081 ggccgatata aataatttat cgttggattc tcagggtcat aataaaaaag cataaatgtt
     1141 attttc
//
LOCUS       DAOPCD010000270         1145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_102466_length_1145_cov_5.911009, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000270 DAOPCD010000000
VERSION     DAOPCD010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102466_length_1145_cov_5.911009"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..>1145)
                     /locus_tag="PLQ55_05275"
     CDS             complement(64..>1145)
                     /locus_tag="PLQ55_05275"
                     /inference="COORDINATES: protein motif:HMM:NF037085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HPK29376.1"
                     /translation="EASLDNLNYHLTNINKSYTIIPLEVVVNWSEADNFVYNGISQKD
                     KVNAYIMGAALEGEIPVTISVDDFTNVGTYEFEASLDNLNYHLTNINKSYTIVKAPLS
                     VIIHEKSSIYFDEITNFTYQIDGTVYQNDDLGIVLSSSVEKGSEVGLYDIVGTFDNDN
                     YELTFIKGTYSILQAEQTLTPLVIDDLSITFHSITVRLIEGVVYSIDGVNYDTNYLFD
                     ELIPLTEYTIYAKLSETRNYHESLPVSIKVTTLADTREFDSIVNGLEDIKIYKSFTMI
                     KRAFAILDEIGAEHFDQELIVDLEAYALSYNQYVDNLTNEIATITEMGNQITKPFDII
                     VVVTGLLNVLFGFLTIMFVKRGRKE"
BASE COUNT          397 a          181 c          145 g          422 t
ORIGIN      
        1 tatgaaagcg gcttatttat tatcagatga aactttttct ttaaattttt tcatccttat
       61 cacctattcc tttcgtcctc ttttaacaaa catgattgtt aaaaacccaa ataagacatt
      121 taataaacct gttactacaa ctatgatatc aaatggttta gtgatttgat ttcccatttc
      181 agtaatcgtt gctatttcgt ttgttaaatt atcaacatat tgattataac ttaaggcata
      241 tgcttctaaa tcaacaatca gttcttgatc aaaatgttca gcaccaattt catcaaggat
      301 agcgaaagca cgcttaatca tggtaaagga tttataaatt ttaatatctt ctaaaccatt
      361 tacaatacta tcaaattcac gggtatcagc tagcgtagtt acttttatgc ttactggaag
      421 tgattcatga taatttcttg tttcactaag tttagcgtaa atggtatatt cggtaagcgg
      481 aattaattca tcaaataaat aattggtatc gtaattaacc ccatcaatac tataaactac
      541 accttcaatt aatctaacag taatagaatg gaaagtgatt gataagtcat ctataacaag
      601 tggtgttaaa gtttgttcgg cttgaagaat agagtatgtc ccttttataa atgttaattc
      661 ataattatca ttatcaaaag ttccaacaat atcatataaa ccaacttcag aacctttttc
      721 aacacttgaa cttaaaacaa ttcccaagtc atcattttga taaactgtac catcaatttg
      781 atatgtaaag tttgtaattt cgtcaaagta gatacttgac ttttcatgaa taatgacact
      841 aagtggtgct ttaactattg tgtatgattt attaatatta gttaagtgat aatttaagtt
      901 atcaagactt gcttcaaatt cataggttcc aacatttgta aaatcatcta ctgatatagt
      961 tactggaatt tcaccttcta aagcagcacc cataatataa gcattaactt tatctttttg
     1021 gctaataccg ttataaacga agttatcagc ttctgaccaa tttacaacta cttctaatgg
     1081 aataattgta tatgatttat taatattagt taagtgataa tttaagttat caagacttgc
     1141 ttcaa
//
LOCUS       DAOPCD010000271         1139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_103316_length_1139_cov_3.011993, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000271 DAOPCD010000000
VERSION     DAOPCD010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103316_length_1139_cov_3.011993"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PLQ55_05280"
     CDS             complement(<1..548)
                     /locus_tag="PLQ55_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29377.1"
                     /translation="MNWLKRNIRYFILSLFLLTNGLIIFEATLAGGTSGARSSLLSLI
                     LSVFVNKTVDGPETAYVPIESLTLKTKDDTTLTEGMHFYIPLGITRRITATVLPENAT
                     DKGVNWTSSDSSALEVYPGGYLEARALSENVLVTAMTNSPLHRISFYVTIHEKVAPPE
                     YEVALTKDRITIDTTTQLEVSV"
     gene            664..>1139
                     /locus_tag="PLQ55_05285"
     CDS             664..>1139
                     /locus_tag="PLQ55_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29378.1"
                     /translation="MKKCQLILLPLLTMLLSGCTPLSLSEVLKPSDNSLSETDVPSTE
                     SSSEIEGAQTWSVDDDFTWQTYEYALDSYNVPLYVPTENPYIDIISDADKETFYTNNY
                     KRASSYEDAMFRTEKGLISGDIIDTPNTANYPLNHLPNRVYRTLANYRINEGVYEYT"
BASE COUNT          387 a          206 c          204 g          342 t
ORIGIN      
        1 tcaacactaa cttcaagttg tgtagtagtg tcaatagtaa tcctatcttt agtaagagcg
       61 acttcgtatt cagggggcgc aactttttcg tgaatagtga cataaaaact aatgcgatgc
      121 agcggactat tagtcatagc ggtgactaat acattttcac ttagtgcgcg cgcttctaag
      181 taaccacccg gataaacttc aagcgcacta ctatcactac ttgtccaatt taccccttta
      241 tccgtagcat tttctggtaa tacagttgca gtgatgcgtc tagtgatgcc tagcggtata
      301 tagaagtgca taccttcagt taaagtagtg tcatctttag tttttagtgt taatgattca
      361 ataggaacat aagctgtttc aggaccgtcc actgttttat taacaaacac acttaaaata
      421 agcgagagta aacttgagcg cgctccagaa gtaccacccg cgagtgttgc ttcaaaaata
      481 ataagcccgt ttgttaataa aaataggctc aagataaagt aacgaatatt tcttttaagc
      541 cagttcatgt ttatattata gcaattagat tgattaaaaa tagtcgcata ctacaactaa
      601 agttgtaatc taacatgttt tagttaataa taaatgacag acttgtcata aagaggttag
      661 atcatgaaaa aatgtcaatt aatattatta ccattattaa ctatgctttt aagcggctgt
      721 acgcctttaa gtttaagtga agtattaaag ccttctgata actccctaag tgaaacagat
      781 gtaccgtcaa ctgaaagtag tagcgaaata gaaggagcac aaacttggtc agtggatgat
      841 gactttactt ggcaaacata tgaatacgct cttgatagtt ataatgtgcc tctatatgtt
      901 ccaacagaaa atccgtatat agatattata agtgacgctg ataaagaaac attttacaca
      961 aacaattata aaagagcatc atcttacgaa gacgcaatgt ttcggacaga aaaaggttta
     1021 atctctggcg atataattga cactccaaac acggcaaact atccgcttaa ccaccttcct
     1081 aatcgagtat atcgcactct agcgaattac cggattaatg aaggagtgta tgaatatac
//
LOCUS       DAOPCD010000272         1104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_108494_length_1104_cov_3.917064, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000272 DAOPCD010000000
VERSION     DAOPCD010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108494_length_1104_cov_3.917064"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="PLQ55_05290"
     CDS             complement(<1..1067)
                     /locus_tag="PLQ55_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HPK29379.1"
                     /translation="MVEILVEKLLAYAELKLALNSEDINYYRNVLLNKFGLKKPYKGV
                     INVSEIQSMNNPQMLIDEMQHLIETKGLPAHLSFADPEEVIIDVLGMMTPIPSVVSQR
                     FAEIHKKDSNKAIKYLYDLSIANNYIQEAKVAENIRWRAELSDKYFEMTINLAKPEID
                     NRETAKLLKEKQDDKYPSCPLCLENVGFGGNESTPPRQNLRAVPLTLDKEEWYLQFSP
                     FVYYKDHIIVISKDHNPMTITPRIFSKLVAFVDQFPDLFIGSNSDIPIVGGSILNHEH
                     FQGGAKVMPMFSAKDRFVLKHKHDRKLKISILEWDSNVIKLESRTKSLLLAKAAEITG
                     NWLEYTNEELGIIPATSGERHA"
BASE COUNT          326 a          210 c          163 g          405 t
ORIGIN      
        1 gcgtggcgtt caccgctagt tgcgggaata atccctaatt cttcattcgt gtattctaac
       61 caattaccag taatttccgc ggcttttgct aaaagaaggc tctttgttct tgattctaat
      121 ttaatgacat tactatccca ttcaagaatg ctaatcttaa gtttacggtc atgtttatgt
      181 tttaagacaa accgatcttt tgcactaaac attggcatca ctttcgcgcc accttggaag
      241 tgttcatgat taagaattga accaccaaca atcggaatat cactattact accaataaat
      301 aaatccggga attgatcgac aaaagccacg agttttgaaa agatacgtgg ggtaattgtc
      361 atcggattat gatctttact aataacgatg atatggtctt tataatacac aaatggtgaa
      421 aattgtaagt accattcttc tttatctaaa gtaagtggca ctgctcttaa attttgccgt
      481 ggtggggttg actcattacc accaaagcca acattttcta aacaaagcgg acaacttgga
      541 tacttatcat cttgtttttc ttttaataat tttgctgttt ctctattatc tatttctggt
      601 tttgctaagt taatcgtcat ttcaaaatat ttatcactta actctgctct ccagcgaata
      661 ttttccgcca ctttagcttc ttgaatatag ttattagcga tacttaaatc atataaatac
      721 ttaattgctt tattactatc tttcttatga atttccgcaa agcgttgact aacaacactc
      781 gggattggtg tcatcatccc taacacatca ataattactt cttcagggtc tgcaaaactt
      841 aaatgtgctg gtaatccttt tgtttcaatt aaatgttgca tttcatcaat taacatttgt
      901 gggttattca tactttgaat ttcactaaca ttaatgacac ctttatatgg tttttttaga
      961 ccaaatttat ttaagagtac attgcggtaa taattgatat cttcgctatt aagagcgagt
     1021 ttaagttccg catacgctaa gagtttttcc actaaaattt ctaccatagt aaatacctcg
     1081 tttccttcct attatcgcaa atat
//
LOCUS       DAOPCD010000273         1095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_109945_length_1095_cov_2.997115, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000273 DAOPCD010000000
VERSION     DAOPCD010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109945_length_1095_cov_2.997115"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..>1095
                     /locus_tag="PLQ55_05295"
     CDS             268..>1095
                     /locus_tag="PLQ55_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPK29380.1"
                     /translation="MKLDTHYNHLKVEKGRYEHWKKSGYFKSGDTSKPKFSVVIPPPN
                     VTGVLHLGHAWDNTFQDIIARYKKAQGFDVLYLPGMDHAGIATQARIDGRLRERGLSR
                     YDLGREKFLEEAWKWKEEYASTIRSQWAKLGLMLDYDHERFTLDKGLNDAVKHTFVEL
                     YNRGYLYLGERIINWDPVFQTALSNIEVIHKDVEGSFYYFKYKLEGSDFYLSVATTRP
                     ETMFADVALVINPRDERYTKYLGKNFVNPVNNELIPLIADDYVDLEFGTGVMKCTPAH
                     "
BASE COUNT          350 a          193 c          245 g          307 t
ORIGIN      
        1 gcataagtaa cttgttgcaa ttaagtgctt aagagtgata aaataaacag gcattgtcat
       61 tacagaagta agattacgag gcgacacaga gagtcgagca aggtgaaagt cgacacgcag
      121 ccacttactg ggtcatagtg actaaagatg tccgggtaat gtccgttatc acatctaatg
      181 aagtgcgtat acatatatac gaaataaggt ggtaccacgg agcaattcgt ccttaaagtg
      241 gtactttttt atttattgga gggaaatatg aaacttgata cccattataa ccatctaaag
      301 gtggaaaaag gtcgttatga acattggaaa aaaagcggct attttaaaag tggtgataca
      361 tctaaaccaa aattcagtgt cgtgatccca cccccgaatg ttactggtgt tttacactta
      421 ggacatgctt gggacaatac attccaagat attattgctc gctataaaaa agcgcaaggg
      481 tttgatgttt tatatttacc aggaatggat cacgctggta ttgctacaca agcgcggatt
      541 gatggacggc ttcgggaacg cggcttatca cgttatgact taggacgcga aaaattctta
      601 gaagaagcat ggaaatggaa agaagaatac gcgagtacga tccgtagtca atgggcaaag
      661 ctcgggctca tgcttgatta tgaccatgaa cgttttacgc ttgataaggg actaaatgac
      721 gctgttaagc atacatttgt ggaactttat aaccgcggtt atctctatct tggtgaacgg
      781 attattaatt gggaccccgt tttccaaaca gcgcttagta atattgaagt cattcataaa
      841 gatgttgaag gctcatttta ttactttaaa tataaacttg aaggcagtga cttttattta
      901 agtgtcgcga caacgcgacc ggaaacaatg tttgccgacg tcgcccttgt cattaaccca
      961 cgcgacgagc gatacacaaa atacttaggt aaaaacttcg taaatccagt aaataatgaa
     1021 ttaattccgt taatcgctga tgattatgtt gatttagaat ttggtactgg tgtgatgaaa
     1081 tgtacgcccg cgcac
//
LOCUS       DAOPCD010000274         1093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_110373_length_1093_cov_1.431599, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000274 DAOPCD010000000
VERSION     DAOPCD010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110373_length_1093_cov_1.431599"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..>1093)
                     /gene="glmU"
                     /locus_tag="PLQ55_05300"
     CDS             complement(138..>1093)
                     /gene="glmU"
                     /locus_tag="PLQ55_05300"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HPK29381.1"
                     /translation="RAPNGEVTRIVEQKDLTGKEGDIREVNAGMYVVNNQKLFAELKN
                     LKPNNKQGELYLTDIIGLFKVKGYKVGAFILANPDEMLGTNDRAQLTEAAELLKKRIN
                     LGHMLSGVTIEDPNNTYIGPDVKIGQDTIIAPGTTILGNSVIGIANEIGPNTYLKNMK
                     IGNNNTIIFSHLVDSEICNNIKIGPFARMRQNALVKDGSRIGNFVELKNTTFHEGVKA
                     GHLAYMGDAELGAHTNVGAGAITANYDGVNKSKTIIGKNVFVGSNTTLVAPLNVGEGS
                     YLAAASIINKDVPKDSLAIARSRQENKEGYAIKIREKQQKK"
BASE COUNT          298 a          220 c          169 g          406 t
ORIGIN      
        1 tatcaattag atatgtaatt attccactat ttgattataa caccttttat tatcacacaa
       61 taggcgatct cctgccgttt taaagtatta aaaaagacgc tttgcaaagg cgtcttttgt
      121 tatttatttc gtttttttta tttcttttgt tgtttttcgc gaatcttaat agcataacct
      181 tctttatttt cttgacgaga acgcgcaatc gcaagactat cttttggtac atctttatta
      241 ataatggatg cagccgcaag ataagagcct tcaccaacat taagaggtgc aactaatgta
      301 gtgttactac caacaaagac atttttacca attatcgttt ttgatttatt aacaccatca
      361 taattggcag ttatcgctcc tgctcctaca ttagtgtgtg ctccaagttc ggcgtccccc
      421 atataagcaa ggtgtcccgc tttaactcct tcgtggaacg ttgtgttttt aagttccaca
      481 aagtttccaa tccgtgaacc atctttcact aaggcatttt gacgcattct agcaaatggt
      541 ccaattttta tattgttaca aatttcacta tccactaagt ggctgaagat aattgtattg
      601 ttattaccaa tcttcatatt ctttaaatat gtgtttggtc caatttcgtt tgcaatccca
      661 attacactgt ttcctaaaat agtagtgcct ggcgcgatta ttgtatcttg gccaatctta
      721 acatctggcc caatatatgt gttattagga tcttcaatgg taacaccact taacatatga
      781 ccaagattaa ttcgtttttt aagaagttcc gcggcttccg tgagttgggc gcggtcattt
      841 gtgcctaaca tttcatcagg attagcaagg atgaacgctc cgactttata accctttact
      901 ttaaataaac caataatatc agttaaatat aattcgcctt gtttgttatt tggttttagg
      961 tttttaagtt cagcaaataa tttttgatta tttacaacat acattcccgc attaacttcg
     1021 cggatatcgc cttctttacc tgtcaaatct ttttgctcaa caatacgtgt tacttcgcca
     1081 tttggcgctc gaa
//
LOCUS       DAOPCD010000275         1081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_112268_length_1081_cov_2.643275, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000275 DAOPCD010000000
VERSION     DAOPCD010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112268_length_1081_cov_2.643275"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PLQ55_05305"
     CDS             complement(<1..1005)
                     /locus_tag="PLQ55_05305"
                     /inference="COORDINATES: protein motif:HMM:NF021352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2357 domain-containing protein"
                     /protein_id="HPK29382.1"
                     /translation="MTDEKKVKKENEEKAIAETEVTTEASAAPKKAKPKTVKKAAPKE
                     KVTKKADAKVASPKEVTKKDTSSKADKKPKKTKTTASKKADDKIAAPKSPKKEIKPET
                     KVLKAKEVKAESEGPAVKEEAPPKAPASKAKSTPRKTRRLSPMELKKREALKGYENFK
                     KVRTRYYSFLRRTVKQSKPTTSLFNTIANVRPELTGITRNEVKRFDAEFIEKIERVIP
                     SLEEIVVNPHKFINEFAEVVQVEKARKLTPRAVKYMAQNAEHVAEVLDDGRVVPKKVL
                     NVFVDDDLKIYENRFIMTLIKRLQIFIELRHKYIREHGDTRNTDQITIQKEVTVDETV
                     F"
BASE COUNT          242 a          217 c          192 g          430 t
ORIGIN      
        1 aaaaactgtc tcatcaaccg ttacttcttt ttgaatcgta atttggtcag tattacgtgt
       61 atcaccatgt tctctaatat atttatgccg caattcaata aagatttgta atcgtttaat
      121 tagtgtcata ataaaacggt tttcataaat ctttaagtca tcatccacga aaacattaag
      181 tactttttta ggcacaaccc gcccatcatc aagaacttca gcgacatgtt cagcattttg
      241 tgccatatat ttgacagcgc gcggggtaag ttttcgcgct ttttccactt gcacaacttc
      301 tgcaaattcg ttaataaatt tatggggatt aacaacaatt tcttcaagcg aaggaataac
      361 ccgttcaatc ttttcaataa attcagcatc aaagcgttta acttcgttac gcgtaatccc
      421 cgttaattct ggtctaacat tggcgattgt attaaataaa ctagtcgtcg gtttactttg
      481 tttaactgtc cgccgtaaaa aagaataata acgagttcgt actttcttaa aattttcata
      541 acccttcaaa gcttcgcgtt tttttaattc catcggtgaa agacgccgcg tttttcgggg
      601 cgtgcttttt gctttcgacg cgggggcttt gggaggcgct tcttctttaa ctgctggtcc
      661 ttcgctttcc gcttttactt cttttgcttt taagacttta gtttctggct ttatttcttt
      721 tttaggagat tttggggccg cgattttatc atctgctttt ttactagcgg tcgttttcgt
      781 ctttttgggc tttttgtctg ctttggaaga agtatctttt ttcgttactt cttttggcga
      841 agcaacttta gcgtcagcct tttttgtaac cttttcttta ggcgcggctt ttttaaccgt
      901 tttgggttta gctttcttcg gcgcagcgct tgcttctgtg gttacttccg tctccgcgat
      961 agctttttct tcattttctt ttttaacttt tttttcgtct gtcattattt acctacgatc
     1021 tggttaagaa ttcatcaatt gtctcgagtg aaagtttaaa attgttttta ccgaatgtgc
     1081 g
//
LOCUS       DAOPCD010000276         1074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_113412_length_1074_cov_5.153091, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000276 DAOPCD010000000
VERSION     DAOPCD010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113412_length_1074_cov_5.153091"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PLQ55_05310"
     CDS             complement(<1..761)
                     /locus_tag="PLQ55_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HPK29383.1"
                     /translation="MFLILSEMLSELQIQVIQLLVVFVFIVTLDVLLLITLKRIFPKV
                     TLIVLSVVLLVLGLFDLTYAFYLVALIYMLCAMTFIFGNIVEFRWLISNVATPKDRRF
                     QRDFRKIYDQEDLVNQLVSAVFSLSKTKTGALITIERTNQLTELIKNGSPIDAPVSAP
                     LLTTIFYKGTALHDGAVVIRDDTILAASVYFTPSTKALAGKVGSRHRAALGISEISDA
                     VTIVVSEETGRISIAYRAALQPVSPEDFRKVLVDH"
BASE COUNT          379 a          197 c          174 g          324 t
ORIGIN      
        1 aaatgatcga ctaatacttt tctaaagtct tcaggactca caggttgcaa agcagcgcga
       61 taagcaatgg aaatacgacc agtttcttca ctaacaacaa tcgtaacagc atcactaatt
      121 tcgctaattc ctaatgctgc gcggtgccgt gagccaacct ttcctgcaag tgctttggtt
      181 gatggtgtaa agtagacact agccgctaaa atggtatcgt cacgaattac aacggcaccg
      241 tcatgaagtg ccgtcccctt atagaaaata gtcgttaata aaggtgcact aaccggtgca
      301 tcaatcggac tgccattttt aattaattcc gttaattgat tagtgcgttc aatagtaata
      361 agcgcaccag ttttagtttt tgataacgaa aataccgcag atacaagttg atttactaaa
      421 tcctcttggt cgtaaatctt acgaaaatca cgctggaagc gacggtcttt tggtgtggcg
      481 acattggaga ttaaccatct aaattcaaca atattaccaa aaataaacgt catagcgcat
      541 aacatataaa ttaaagcaac taagtaaaag gcatatgtta aatcaaataa accgagcact
      601 aacaagacaa ctgaaagtac gataagcgta actttgggga atatacgttt aagcgtaatt
      661 aataaaagta catcaagtgt aactataaaa acaaaaacaa ctaagagttg tataacctga
      721 atttgcaatt cactaagcat ttctgaaagt attaaaaaca taataaatcc cccgtctatt
      781 tgtataaata ttttagcatg taaacacaac cattttcaat ttaaaatgtg taaattgtcg
      841 ctatttttta acgaaaacta atcccaaatt aaaaagagtg cctttttgca aggttatcgc
      901 aataaacatt ataattattt cggcaatata atactaaaca ttatgttcga atgttattta
      961 aaatatctga gcattaagcg cgtcttttaa gtaattcact tttaattttg tccgcagctt
     1021 ctctttgttt aagccaaaac tctttacttt cttcgaggtt aaattcacgg ggta
//
LOCUS       DAOPCD010000277         1074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_113480_length_1074_cov_2.435721, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000277 DAOPCD010000000
VERSION     DAOPCD010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113480_length_1074_cov_2.435721"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PLQ55_05315"
     CDS             complement(<1..602)
                     /locus_tag="PLQ55_05315"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPK29384.1"
                     /translation="MIRIGDLIDNRYRIVSLLGEGGMGTVYEAVDIITKRNVALKFLK
                     ANVARDKVNLARFEIEAKATATLNHPNIVRVISVGKYENLPYMVNELIVGNNLEDELQ
                     TRGRYTFLEAADIIDQLCNAVSLAHKNGVIHRDIKPSNIYITKEGLVKLGDFGIARYS
                     DSKRVTSNTNIVGSAHYLAPEVCRGGEASPRSDIYALGIN"
     gene            complement(595..>1074)
                     /locus_tag="PLQ55_05320"
     CDS             complement(595..>1074)
                     /locus_tag="PLQ55_05320"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HPK29385.1"
                     /translation="IFEESILSPDMNNMSTTLNAVVLTRSKLCNVNIGDSRFYLCTDD
                     EMYLMSEDQTVVGYLVRTNQITPEEALVHPKRHILTNALGTMPTVTFDFRKFQYRGER
                     ILVCSDGLYNMLDSEDILGILRTTQTAEFKVDALVALANHRGGADNIGVALWEPDND"
BASE COUNT          327 a          236 c          174 g          337 t
ORIGIN      
        1 aaattaatac ctaaagcata aatatcactg cgtggtgaag cttcgccacc acgacacact
       61 tcgggcgcta agtaatgggc gctaccaaca atatttgtgt tacttgtgac acgctttgag
      121 tcactataac gagcaatacc aaagtcccca agtttaacaa gtccttcttt agtaatataa
      181 atattacttg gttttatatc acgatgaatt accccgtttt tatgcgctaa actaacagcg
      241 ttacacagct gatcaataat atcagcagct tctaaaaatg tatagcgacc gcgcgtttgc
      301 agttcatctt ctaagttatt accgactatt aattcattca ccatgtaagg taaattttca
      361 tacttgccta cactaatgac acgaacaata tttggatgat ttaaagtcgc agttgctttg
      421 gcttcaattt caaaacgggc aagattcact ttatcacgag cgacattagc ctttaaaaac
      481 ttcagcgcga cattacgttt ggtaatgatg tcgactgctt cataaactgt tcccattccg
      541 ccttcaccaa gcaaacttac aatccgatat cgattgtcaa ttaaatcacc tattctaatc
      601 attatctggc tcccataatg ctactcctat attgtcggct ccaccgcgat gattggcgag
      661 agcgacgagt gcgtctactt taaattccgc tgtttgtgtt gtgcgtaata ttcctaaaat
      721 atcttcgcta tcaagcatgt tgtaaagacc atcactacac actaaaattc gttcaccccg
      781 atattgaaac ttcctaaaat caaaagtaac agttggcatc gtaccaagcg catttgttaa
      841 gatgtgacgt ttggggtgca ctagtgcttc ttcgggggta atttgatttg tgcgcactaa
      901 ataaccgaca actgtttggt cttcactcat taaatacatt tcatcatccg tacataaata
      961 aaaacgacta tcaccaatat taacattaca caattttgac cgcgttaata ctacagcatt
     1021 taatgttgtt gacatattgt tcatatctgg ggataaaata ctttcttcaa agat
//
LOCUS       DAOPCD010000278         1069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_114336_length_1069_cov_2.399408, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000278 DAOPCD010000000
VERSION     DAOPCD010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114336_length_1069_cov_2.399408"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLQ55_05325"
     CDS             <1..336
                     /locus_tag="PLQ55_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPK29386.1"
                     /translation="FILSLFPNVPLWVQIIAFIATSGILLLLSFLFFRKSFLNAKSVQ
                     TNVDAMLEEEVILLSSCDKDTLGEVKYRDVIWKVTPKSHDASFEKGETAIVDGIKGNR
                     LVIKKKGTK"
     gene            339..>1069
                     /locus_tag="PLQ55_05330"
     CDS             339..>1069
                     /locus_tag="PLQ55_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009272133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPK29387.1"
                     /translation="MKTVKFLLTDGQIFGIIALAVLLLFIFIVLIRSIKIVRQATVLV
                     VERLGKYNRICHAGIHFIIPFFERASQPIFLKERVSDFPPQPVITKDNVTMSIDTVVY
                     WQVTDPKLFMYGVISPQSALENLTATTLRNLVGDLELDDTLTSRDTVNHQMWEVLDLA
                     TDPWGIKVTRVELKNILPPRDIQEAMEKQMRAERQKRQEILEAEGKKQAAILIAEGEK
                     EAVILRAEAAKQKMIKEAEGEAAALL"
BASE COUNT          355 a          188 c          206 g          320 t
ORIGIN      
        1 tttattctta gtttattccc aaatgtccca ctttgggtac aaataattgc ctttattgcc
       61 acaagcggaa tattattact acttagtttt ctctttttcc gcaagtcatt tttaaatgct
      121 aaaagtgtgc aaactaatgt tgatgctatg cttgaggaag aagtaatctt actatcttca
      181 tgcgataaag acacacttgg cgaagtaaaa tatcgtgatg ttatttggaa agtgacacca
      241 aaaagtcatg acgctagttt tgaaaaaggc gaaaccgcaa ttgttgatgg aatcaaaggt
      301 aatcgcttag ttataaaaaa gaaaggaact aaataaatat gaaaactgtt aaattcttac
      361 ttactgatgg gcaaattttt ggaattattg cccttgctgt cttattacta tttattttta
      421 ttgtgctaat tcgttcaatt aaaattgtac gccaagcaac tgtcttagtc gttgaaagac
      481 taggtaaata caatcgtatt tgtcacgctg gcattcactt tatcattccc ttttttgaac
      541 gagcaagtca accaatcttc ttaaaagaaa gggtgagtga cttcccaccg caacctgtta
      601 ttactaaaga caatgtcaca atgagtattg acactgttgt gtattggcaa gtaactgatc
      661 caaaattatt tatgtacgga gttatctctc cgcaaagtgc attagaaaac ttgaccgcta
      721 cgacattacg taacttagtg ggggacttag aattagatga tacactcaca agtcgtgaca
      781 ctgttaatca tcaaatgtgg gaagttcttg accttgcaac tgacccatgg ggtattaaag
      841 taacacgtgt tgaacttaaa aacattcttc cacctagaga tattcaagaa gcaatggaaa
      901 aacaaatgcg cgcggaacgg caaaaacgcc aagaaattct tgaagctgag ggtaaaaagc
      961 aagccgctat tttaattgct gaaggggaaa aagaagctgt tatcttacgc gctgaagccg
     1021 caaagcaaaa aatgattaag gaagcggaag gtgaagctgc tgcccttct
//
LOCUS       DAOPCD010000279         1062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_115521_length_1062_cov_2.370407, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000279 DAOPCD010000000
VERSION     DAOPCD010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115521_length_1062_cov_2.370407"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLQ55_05335"
     CDS             complement(<1..332)
                     /locus_tag="PLQ55_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29388.1"
                     /translation="MSKLKIKSLNKKSFAQQSQYYLIIGLIIIVSFLFIKPLFSAQAQ
                     EDAQTEASEESTILNIKKVIQDKQVELGQGEQRTGQLKQAYLAQVKRVSAETITVLNR
                     NVNVVIPLT"
     gene            complement(334..852)
                     /locus_tag="PLQ55_05340"
     CDS             complement(334..852)
                     /locus_tag="PLQ55_05340"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29389.1"
                     /translation="MRKEVLLAILIGASLGLLITFAVYQSRRSVSEAQKDQVQEVLNN
                     HTDNEEVDTSQLAITSPEDNSIVDSQRLIVSGNTQANNFIVIFVNNEEIITNADDSGN
                     FSKEVRLDEGANVLVIYAINQDGLVSQNTRTVIVDADFLNEDFTNPTTAEEAIDDNQA
                     TAEADQEKDTEE"
BASE COUNT          297 a          205 c          188 g          372 t
ORIGIN      
        1 gttaggggaa tgaccacatt aacatttcta tttaggacag tgatagtttc ggcgctgact
       61 cgtttgactt gcgctaaata cgcctgtttg agctgtcccg tgcgttgctc accttgacct
      121 agttccactt gtttatcttg tataactttt ttaatattta aaatagtcga ctcttcactg
      181 gcctcagttt gtgcatcttc ttgtgcttgc gcgctaaaca gcggcttgat gaataaaaaa
      241 ctaacaatga tgattaagcc aattattaga taatattgac tctgttgtgc gaaacttttt
      301 ttatttaaac tttttatctt caactttgac atattattct tcggtgtctt tttcttgatc
      361 agcttcagct gttgcttggt tatcatctat cgcttcttca gcagttgtgg gattggtaaa
      421 atcttcattc aaaaaatcag catcaacaat tacggttcgt gtgttttggg aaactaagcc
      481 gtcttgatta atggcataga taaccaaaac attggctcct tcatcgagac gaacttcttt
      541 agaaaaatta ccagaatcat cggcattggt aatgatttct tcattattta caaaaataac
      601 tatgaagtta tttgcttgcg tattgccact gacaatcaaa cgctgactat caacgatcga
      661 attatcttct ggagaagtaa tggccagttg actagtgtct acctcttcat tatcagtatg
      721 attattcagc acttcttgga cttgatcttt ttgcgcttca ctaacgctac gacgactttg
      781 ataaacagca aaagtaatta acagacctaa actggccccg attaagattg ctaaaagaac
      841 ttcttttcgc atatgactat tataattgaa gagtcatcct atgttaaatc ttgtaaaaag
      901 tcaactttaa cggtaatatg cgattatgca aattagctat tttggtcatt cttgtttcaa
      961 gttaaaaggt aaacacggta cactggtcac tgatccttat agtgccgaag gagtttatgg
     1021 tattagtatg cctcagctca ccgccgactt agtgaccgtt tc
//
LOCUS       DAOPCD010000280         1062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_115548_length_1062_cov_2.096326, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000280 DAOPCD010000000
VERSION     DAOPCD010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115548_length_1062_cov_2.096326"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..791)
                     /locus_tag="PLQ55_05345"
     CDS             complement(3..791)
                     /locus_tag="PLQ55_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPK29390.1"
                     /translation="MKDIFAALRPKVNENIKKAIAAGDLNAKVMPGDHVVTKAERAKY
                     VVTYDLEKKTLRSKVKRIFGNIAANKKTRDIAKYIETNGLENALPLKGKAFILTSNHY
                     SPYDNLIARHLTNKLGYKNKLSIIINESNIFMPGKLGKILRSVDVLPYAQDFEYLGKR
                     FNPAIGNALKKKRVVLIYPEQEMWLDYPRTRPLKPGAYHYAAKFNVPILPIFVTWKKD
                     EEGVTKYIINIGKPIYSDTKLSLAVNKKNMLTADDSFKKSISPK"
     gene            complement(796..>1062)
                     /locus_tag="PLQ55_05350"
     CDS             complement(796..>1062)
                     /locus_tag="PLQ55_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29391.1"
                     /translation="DPEFDYTTLKLVHYNSFEKPWHHDHVLYADKFWEAAEGSPFFED
                     LKKIRENVGEAHLQKEEKGVKALVEKATYLATTEDVTFKKVLAL"
BASE COUNT          302 a          196 c          170 g          394 t
ORIGIN      
        1 atttactttg gactaatact ctttttaaag gagtcatcag cggtaagcat attcttctta
       61 ttaactgcaa gactaagctt agtgtcacta tatatcggct taccaatatt aataatgtac
      121 ttagtaacgc cttcttcatc ttttttccat gttacaaaaa taggaaggat cggaacatta
      181 aacttagcag cgtaatgata cgcgcctggt ttaagtggtc tagtacgcgg gtaatctaac
      241 cacatttctt gttctggata gattaagact actctttttt tctttagagc atttccaata
      301 gcgggattaa atcttttgcc taaatattca aaatcttgag cgtaaggtaa aacatcaaca
      361 ctgcgaagta ttttacctaa tttccctggc ataaagatat tgctttcatt aataataatg
      421 cttaacttat ttttataacc gagtttattt gttaagtgtc tcgcaattaa gttgtcgtag
      481 ggagaatagt gattgcttgt taaaataaac gctttacctt ttaaaggcaa ggcgttttct
      541 aaaccattag tttcaatata tttagcgata tctcttgttt ttttattagc ggcaatattt
      601 ccgaatatcc gtttaacttt actgcgtaag gttttctttt ctaagtcata agtaacaaca
      661 tattttgctc gttctgcttt agtaacaacg tggtctccag gcattacttt cgcatttaaa
      721 tcaccagcgg caatggcttt ttttatgttt tcatttactt tgggacgtaa ggccgcgaaa
      781 atatctttca taaaactata acgctaagac ctttttaaaa gttacatctt ccgtagtcgc
      841 taagtaagta gctttttcca ctaaggcctt cactcctttt tcttcttttt gtaaatgagc
      901 ttctcccaca ttttctctaa tcttttttaa gtcttcaaag aaagggctac cttcagccgc
      961 ttcccaaaac ttatctgcgt acaagacatg atcatggtgc caaggttttt caaatgaatt
     1021 atagtggaca agttttaatg ttgtgtaatc aaactctggg tc
//
LOCUS       DAOPCD010000281         1058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_116146_length_1058_cov_3.977069, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000281 DAOPCD010000000
VERSION     DAOPCD010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116146_length_1058_cov_3.977069"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PLQ55_05355"
     CDS             complement(<1..556)
                     /locus_tag="PLQ55_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HPK29392.1"
                     /translation="MKKLTSNDIRKMWLSFFKSKNHAVVEGASLIPVNDKTLLWINSG
                     VAALKQYFDGSKVSADKRLTNVQKSIRTNDIENVGKTNRHHTFFEMLGNFSIGDYFRT
                     EAITWGFEFLTSKDYLALPLEKLYFTYHPSDTETRDLWISLGVPKSHVIANEDNYWEI
                     GEGPCGPNTEIFFDLGEKIDARGPE"
     gene            complement(786..>1058)
                     /locus_tag="PLQ55_05360"
     CDS             complement(786..>1058)
                     /locus_tag="PLQ55_05360"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPK29393.1"
                     /translation="EHLKSDKITLTDVNFINEELKSGTPLNVKLRYRQKDLPATYYKN
                     KDGAYLLFPKPYLAVTPGQAAVFYIDEVCLGGAIIGDLFYKGKKIN"
BASE COUNT          321 a          219 c          170 g          348 t
ORIGIN      
        1 attctggacc acgcgcatca attttttccc ctaaatcaaa gaaaatttcg gtattgggac
       61 cacacggtcc ttccccaatt tcccaataat tatcttcatt agcgataaca tgactcttcg
      121 gcacacctaa actaatccat aagtcacgcg tttctgtgtc acttgggtga taagtaaaat
      181 aaagtttctc aagcggtaaa gctaagtaat ctttactcgt taaaaattca aagccccagg
      241 taattgcttc cgtacggaaa tagtcaccaa tactaaaatt acctaacatc tcaaaaaagg
      301 tgtggtgacg atttgtttta ccgacatttt caatatcatt cgtgcgaata cttttttgga
      361 catttgttaa acgtttatcc gcacttacct tactaccatc aaaatattgt ttaagcgctg
      421 cgacgcccga attaatccaa agtagtgtct tatcattaac cggaatcaaa ctagcgcctt
      481 ccactacggc atgatttttg cttttaaaaa aggacagcca cattttgcga atgtcattac
      541 ttgttaattt tttcataaaa aacctcaact aacataggaa cgactctcgc cgcggtacca
      601 tcctaattca tcgttgtgat gcgctttatt ttaattttgt aacggtaatt aaccggcgat
      661 gattagtcgc tcgaaggaag tggcacgttt tacttttgag tcctttcacc aactaactct
      721 cgcttttaag tttgtaaaac ttagtttcct tattgatttc ggtaatatta tagcatagtt
      781 aacgattagt taatcttttt gcctttataa aataagtcac caataatagc accgccaaga
      841 cacacttcgt caatatagaa aactgctgct tgacctgggg tcacggctag ataaggtttg
      901 gggaataata agtaggcacc gtctttattt ttataataag tggcaggcaa gtctttttgc
      961 cgataacgga gcttaacatt taagggagtt ccactcttta attcttcatt aataaaatta
     1021 acatcagtca aagtaatctt atcacttttt aaatgttc
//
LOCUS       DAOPCD010000282         1056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_116519_length_1056_cov_2.907093, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000282 DAOPCD010000000
VERSION     DAOPCD010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116519_length_1056_cov_2.907093"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..>1056)
                     /locus_tag="PLQ55_05365"
     CDS             complement(142..>1056)
                     /locus_tag="PLQ55_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29394.1"
                     /translation="VQKSFGFGSLAKYAATRADNEFDIETTVDYVAVVLEADVIKNLR
                     LDTVQLYAKIAEEAVVLKTETNGDVKSKWELLDGYGMKVASPIEKEWYEQAEAFENWA
                     VGKTVAEVKAGMTGTKLTDGATIGNTIHVNAWVAALEDAVLNKVTFEGEVKGLGVGGV
                     NAVSKDGATGAVEGQDWTIGGAVFTEGKKVLAARIDTFQVRYENSEGKAVIKSGLKQV
                     DYDNKRVKGKQELKEEYGMKVASPIEKEWYEQAASLVEYIKGKTVSEALGTAAKLANG
                     AEVGCTMNIGGYRTALLEAEHTAFNSRK"
BASE COUNT          293 a          235 c          162 g          366 t
ORIGIN      
        1 aggaaggtgg taaaatctat cttgttttat atcaaaaatg tttttttctt tttctttccc
       61 atagtaatat tatcgcatat taacaaaaaa ggaactactt tgaagtagtc ccttgtgtat
      121 cttttcttcg taactaatta attatttacg actattaaag gctgtgtgtt cagcttcaag
      181 taaagcagtg cgatagccac caatattcat agtacaacca acttcagcac catttgctaa
      241 tttagcagca gttcctaaag cttcgctaac ggttttacct ttaatgtatt cgactaagct
      301 tgcagcttgt tcgtaccatt ctttttcgat tgggcttgca actttcatgc catattcttc
      361 tttaagttct tgtttacctt ttacgcgttt attatcataa tcaacttgtt ttaagccaga
      421 tttaataact gctttacctt cactattttc atagcgaact tggaaagtat caatgcgtgc
      481 cgctaagact tttttgcctt cggtaaagac agcaccacca atggtccagt cttgtccttc
      541 aacagcacca gttgcgccgt ctttgctaac agcgttaaca ccaccaacac ctaatccttt
      601 aacttcacct tcaaaagtaa ctttattaag gacagcgtct tcaagggcgg caacccaagc
      661 gttaacatgg attgtattac caattgttgc accatcagtt aatttcgtac cagtcatgcc
      721 agctttaact tcggcaactg ttttaccaac ggcccaattt tcaaatgctt cagcttgttc
      781 gtaccattct ttttcgattg ggctcgcaac tttcatgccg taaccatcta aaagttccca
      841 tttagattta acatcaccat tagtttcggt tttaagtaca actgcttctt cagcaatttt
      901 tgcataaagt tggacagtgt caagccgtaa gtttttaata acatctgctt ctaagacaac
      961 agcgacataa tcaacagttg tttcaatatc aaattcatta tcagcgcgtg ttgcggcata
     1021 ttttgcaaga ctgccaaaac caaatgattt ttgcac
//
LOCUS       DAOPCD010000283         1054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_116886_length_1054_cov_2.648649, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000283 DAOPCD010000000
VERSION     DAOPCD010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116886_length_1054_cov_2.648649"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PLQ55_05370"
     CDS             <1..632
                     /locus_tag="PLQ55_05370"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPK29395.1"
                     /translation="EIGVNNSIDPMVFVDEDERVYMFWGSFRGIYAIELTADGLGFKD
                     GSVEQAANNKVHIAGFETNRNFDISTFEGVYIIKKDNYYYMFLSTGTCCSGDYTYNVV
                     VGRSESILGEYFDSYNRSMKNANVGHSVVHQNDFFIGVGHNSILRDDAGTYWILYHGF
                     DATVETRNARKLLIDKLIWNADNWPSTLGNVPSNNYRPGPELYLSDENN"
     gene            864..>1054
                     /locus_tag="PLQ55_05375"
     CDS             864..>1054
                     /locus_tag="PLQ55_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29396.1"
                     /translation="MKKHRYLVLALLSLVLLGSCKGDGSESGKTSEPQVSTTSTVGSE
                     EGERTITWYGTDDIVITIG"
BASE COUNT          359 a          139 c          219 g          337 t
ORIGIN      
        1 aagaaattgg tgtaaataat agtattgacc caatggtgtt tgtcgatgaa gatgaacgtg
       61 tttatatgtt ttggggtagt tttagaggta tatacgcaat tgaattaaca gcggatggcc
      121 ttggttttaa agacggaagt gttgaacaag cagcgaataa taaggtgcat atcgctggtt
      181 ttgaaacaaa tcgtaacttt gatatatcga ccttcgaagg tgtatacatt attaaaaaag
      241 ataattacta ctatatgttt ttatcgactg gtacatgttg ttctggtgat tatacttata
      301 atgttgtcgt tgggcgaagt gaaagtattt taggggaata ctttgattca tataatcgta
      361 gtatgaaaaa cgctaatgtt ggacattccg tcgtccatca aaatgatttc tttattggtg
      421 ttggtcataa ttcaatcttg cgtgatgatg ctggaacata ttggattctt tatcacggat
      481 ttgatgcaac agtggaaacg cgtaatgccc gcaagttatt aattgataaa ctcatatgga
      541 atgctgataa ttggccgagt acattaggta atgtgcctag taataattat cggccaggac
      601 ctgaattata cttaagtgat gaaaataatt aatgacgaat taattagttt gtccttgact
      661 atttttgcta cgctttataa aataaaggtg taagtaagat taacgataat ctaaaaaaca
      721 aaatcatacc aataattcaa taaaaataga tcgaaattag tgccccacaa cttaaacttc
      781 agaaactacc atgatagttt cgtatcctta tgtttaatta aataattgat ggtaaataaa
      841 tcaaaggaaa aggagacttg attatgaaaa aacatcgcta tctagtgttg gcactacttt
      901 cattagtgtt acttggctct tgtaaaggtg atggtagtga aagcggaaag acaagcgaac
      961 cgcaagtaag tacaacatct acagttggtt cagaagaagg tgaacgaaca attacgtggt
     1021 acggcactga tgatattgta attacgattg gaga
//
LOCUS       DAOPCD010000284         1040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_119495_length_1040_cov_1.924873, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000284 DAOPCD010000000
VERSION     DAOPCD010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119495_length_1040_cov_1.924873"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="PLQ55_05380"
     CDS             complement(<1..981)
                     /locus_tag="PLQ55_05380"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPK29397.1"
                     /translation="MKIKRHVRHLMVLVFMMVISSCALFPTSSEKDSLPIASSSSETF
                     SSANESSEELSTSEVPSSESEVQSSEPEPSSKELSSSEEPSISVELEMKRSTYTNSVY
                     FYDFPDPSGVYDPSQRMWFLYATGGQYLRSSDGVTWGQKGWVFGHLPDWGTKGAGLWA
                     PDVQYIYGQYVMYYSLSTWGDPNPGIGYATAPKPNGPWTDHGKLFLSQEIGVNNSIDA
                     NAFVDPNTNRVYLVWGSMRGNYLVELTRDGLGFKAGSVEQAALSKIRVAGLDTVHGWD
                     AETYEAAYIRYWDGYYYLFLSMGTCCEGLSSTYKVIVGRALSPYGPYYDDT"
BASE COUNT          305 a          249 c          177 g          309 t
ORIGIN      
        1 tgtatcatca taataaggac catatggtga aagggcgcgc ccgactatta ctttgtaagt
       61 agagctaagt ccttcacaac atgtacccat tgataagaat aaataatagt agccatccca
      121 ataacgaata taggctgctt cgtaagtttc agcgtcccag ccgtgcaccg tatctaagcc
      181 cgcgactctt attttactaa gtgccgcctg ttcaacgctt cccgctttaa acccaagacc
      241 gtcacgcgta agctccacta aatagttacc acgcattgaa ccccacacta aataaacacg
      301 gttagtgtta ggatcaacaa aagcatttgc gtcaatacta ttattcacac caatttcttg
      361 tgataaaaat aatttaccgt gatcagtcca cggcccattt ggttttggcg cagtagcgta
      421 accaatcccc ggattaggat cgccccatgt tgaaagtgag taatacatta catattgtcc
      481 gtatatatat tgcacatctg gtgcccaaag tcctgctcct tttgtccccc aatctggtaa
      541 atgaccaaag acccaacctt tttgacccca agttacgcca tcgctcgagc gtaaatattg
      601 gccacctgtt gcatataaaa accacatgcg ttgacttggg tcatatactc ctgaagggtc
      661 agggaaatca taaaaataca cagagttcgt gtatgtactt cttttcattt caagttccac
      721 gctaatacta ggctcctcac tacttgataa ctctttacta cttggttcag gttcactgct
      781 ttgcacttcg ctttcacttg agggtacttc gcttgttgat agctcttcac tactttcatt
      841 tgcactcgaa aacgtttcac tactacttga agcaattggg agcgaatctt tttcactact
      901 tgtagggaaa agtgcacaac tagaaattac catcataaat actaagacca ttaaatggcg
      961 aacatgtcgt tttattttca taagcgcccc ctattatagt ttttcaaagg ttatcgttaa
     1021 tctaattata aaacgaaaaa
//
LOCUS       DAOPCD010000285         1037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_119939_length_1037_cov_3.000000, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000285 DAOPCD010000000
VERSION     DAOPCD010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119939_length_1037_cov_3.000000"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..>1037
                     /locus_tag="PLQ55_05385"
     CDS             66..>1037
                     /locus_tag="PLQ55_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPK29398.1"
                     /translation="MMETKHGTTYNSYLIKEEGGVTIIDGVKAGFTDKWLDNIRSLVD
                     IKDIKYLVVQHTEPDHSGAIPALLALNPNIEVYGSFMAMANLKEINNAPFISNVIKPK
                     QELKLSERQSLFFYPVPNVHWPDTIITHDPVNAIAFTCDFLGQHIAPQQLLYSKEPNK
                     KIIEEEIKLYYDAIMAPFKSDVLKSVATLKSLDVSMVLNSHGYILDTKEAVDWAINNY
                     IKWSTVNKSEKLTVTVVFASAYGYTRSMSEAVISELEKASALVHVFEVPRDDLATIVA
                     RIEESHALLVASPTFVGDGIKPVYDVLNSLNPVMCFGKYFSAFGSYGW"
BASE COUNT          339 a          152 c          213 g          333 t
ORIGIN      
        1 ttaatgtaaa aaataatgtt tattatattg gagttagtga tagaggactt cgtgtgtttg
       61 atattatgat ggagacaaaa cacggcacaa catataactc ttatttaatt aaagaagaag
      121 gtggagtgac gattattgac ggagttaaag caggatttac tgataagtgg ttagacaata
      181 tacgttcatt agtcgatatt aaagatatta aatatttagt cgtgcaacat actgaacctg
      241 accatagcgg cgctattcct gcccttttag cgcttaatcc aaatattgaa gtttacggtt
      301 catttatggc aatggctaat ttaaaagaaa ttaataatgc accatttatt agtaatgtaa
      361 ttaaaccaaa acaagaactt aaacttagtg agcggcaaag tttatttttc taccctgtgc
      421 caaacgttca ttggccggat actattatta cccatgaccc agttaacgcg attgctttta
      481 cttgtgactt tttagggcaa catattgctc cacaacaatt actttatagt aaagaaccta
      541 ataaaaagat tattgaagaa gaaattaaac tatattacga cgcaattatg gcacccttta
      601 aaagtgacgt gcttaaaagt gtggcgactt taaaaagttt agatgtaagt atggtgctta
      661 atagtcacgg ttatatttta gacacaaaag aagctgttga ttgggcgatt aataactata
      721 ttaaatggtc aactgtaaat aaaagtgaaa aattaacggt tactgtcgtc tttgctagtg
      781 cttatgggta tacgaggagt atgagtgaag ccgttattag tgaattagaa aaagcgagtg
      841 ccttagttca tgtttttgaa gtacctcgtg atgacctagc aacaattgtg gcacgcattg
      901 aagaaagtca tgcgctccta gttgcttcac caacctttgt cggggacggg attaaaccag
      961 tgtatgatgt cttgaatagt cttaatccgg tgatgtgctt tggtaaatat tttagcgctt
     1021 ttgggagtta tggctgg
//
LOCUS       DAOPCD010000286         1037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_119950_length_1037_cov_2.817719, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000286 DAOPCD010000000
VERSION     DAOPCD010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119950_length_1037_cov_2.817719"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1037
                     /locus_tag="PLQ55_05390"
     CDS             <1..>1037
                     /locus_tag="PLQ55_05390"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29399.1"
                     /translation="AELDFEKARDYKEIVDAMLHIYSSQNIELKNKVDLDVIAFTQIN
                     DYIGLNIVSYRAGVLLNEQFDAFPIFSDLHEQIINLIGQYYTKNPLPTKLMVGNSFIA
                     EALTLYYDKEVIVPSRGQNLEILNRAYQNATSALQKHLLYHQALGTENESLTALSETL
                     GIKYPSHIDFVDIAHLFGSNAQGVVITYINGRPFKKLYRKYNIENENSFDDYASIKEV
                     LTRHYKRKLIEGKHLPDLLIVDGGKGQLGVALSVKDELNLDFNIISLVKDDKHTTKAL
                     INEEGDEISVNKNLLTFLAAIQNEGHRYAITSHRAKRGKGTYTSALDGIKGLGKKRKM
                     LLLNTYKSIDV"
BASE COUNT          362 a          164 c          191 g          320 t
ORIGIN      
        1 tgcggaactt gactttgaaa aagcgcgtga ttataaagaa atagttgacg cgatgctcca
       61 tatttatagc agtcaaaata ttgaactaaa aaataaagtt gatttagatg taattgcctt
      121 tacccaaatt aatgattata ttgggttaaa tattgtctct tatcgtgctg gagtattact
      181 taatgaacag tttgatgcct tccccatatt tagcgacctt catgaacaaa ttattaattt
      241 aattgggcaa tactacacaa agaatccatt gcccactaaa ttaatggtcg gtaattcatt
      301 tatcgctgaa gcgttaacgc tttattacga taaagaagtt attgtcccta gtcgcggaca
      361 aaatctagaa atccttaatc gggcttatca aaatgccaca agtgcattgc agaagcattt
      421 actatatcat caagctttag gcactgaaaa tgaaagttta acggcgctaa gtgaaacgct
      481 tggtattaag tatcctagtc atattgattt tgttgatatt gctcatcttt ttggtagtaa
      541 tgcccaagga gtcgttatta cttatattaa cggtcgacca tttaagaagt tatatcgtaa
      601 atataatatt gaaaatgaaa attcttttga tgattacgct agtattaaag aagtactcac
      661 acgccactat aaacgaaaac tcattgaagg aaaacattta cctgatttac taatcgttga
      721 tggcggtaaa ggtcaacttg gtgttgcgct tagtgtgaaa gacgaattaa atcttgattt
      781 caacattatt agccttgtga aagatgataa acatacaaca aaagcactta ttaatgaaga
      841 aggtgacgaa ataagtgtta ataaaaattt actcactttt ctcgccgcga ttcaaaatga
      901 agggcaccgt tacgcgatta ctagtcacag agctaaaaga gggaaaggga catacacaag
      961 cgctttagac gggattaaag gtttaggcaa gaaacggaag atgctcttac taaatactta
     1021 taaaagtatt gatgtct
//
LOCUS       DAOPCD010000287         1032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_120979_length_1032_cov_4.138178, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000287 DAOPCD010000000
VERSION     DAOPCD010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120979_length_1032_cov_4.138178"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..>1032
                     /locus_tag="PLQ55_05395"
     CDS             18..>1032
                     /locus_tag="PLQ55_05395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK29400.1"
                     /translation="MKILIVFNHPAPYKVALFNGLSAKHDVHVIFERFKASNRNQSFY
                     NESNYKFTLHKIKGISLGDENHLSFGVVRHLKKHKYDLIIMNGYSTLTEMLALRYLKR
                     KKIPYAFYINGGFANEKECKLKREIKTYFISGAEAYFSPAKKANEYLTYYGAPADRIY
                     NYPYSTVYEKDVLEVKTPTATKVLYWKEKGIEATNFTICITSFIKRKNNEALIKAWKE
                     IPKDYALILVGEGYEEPMYREIIANEKLTNVLLLPFAPKHLVFEYLSHSDNAVYISNY
                     DIYGHVINEALAHGLNVLTNTKMVAAIDLIRDGQNGLIAYPEDDLVSRILLLLATDFF
                     NEAI"
BASE COUNT          364 a          159 c          175 g          334 t
ORIGIN      
        1 ttgtataatt tatacatatg aaaatattaa ttgtgtttaa tcatcctgcc ccatataaag
       61 tcgcactctt taatggttta agtgcaaaac atgatgtgca tgttattttt gaacgcttta
      121 aagcaagtaa tcgtaatcaa agtttttaca atgaatcaaa ttataaattt accctccata
      181 aaataaaggg tatctctctt ggcgacgaaa atcatctttc gtttggtgta gtacgtcact
      241 taaagaagca taaatacgac ttaattatta tgaatggtta ctcaacatta acagaaatgc
      301 ttgcacttcg ttatttaaag cgtaaaaaaa ttccttatgc tttttatatt aatggcggtt
      361 tcgctaatga gaaagaatgt aagcttaaaa gggaaattaa aacttatttt attagcggcg
      421 cagaagccta ctttagtcct gctaaaaagg caaatgagta cttaacttat tacggagcgc
      481 ccgctgatag gatttataat tatccttact ccacagttta tgaaaaagat gttttagaag
      541 ttaaaacacc aaccgctact aaagttttgt attggaaaga aaaaggaatt gaagcaacta
      601 attttacgat ttgtataact agttttatta aacgtaaaaa taatgaagcg cttattaaag
      661 cgtggaaaga aataccaaaa gactatgccc tcattctagt aggtgaaggc tatgaagaac
      721 cgatgtatcg cgaaattatt gcaaatgaga aattaacaaa tgtacttctg cttccgtttg
      781 cgcctaaaca ccttgtattt gaatatttat cacatagtga taatgctgtt tatattagta
      841 attacgatat ttatggtcat gtcattaatg aagcactagc gcacgggctt aatgttttaa
      901 caaatacaaa aatggtggcc gcaattgact taattcgtga cggacaaaac ggtttaattg
      961 cttatccaga agacgattta gtaagcagaa tattactact gcttgcgact gacttcttta
     1021 atgaagcaat ca
//
LOCUS       DAOPCD010000288         1028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_121766_length_1028_cov_4.164440, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000288 DAOPCD010000000
VERSION     DAOPCD010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121766_length_1028_cov_4.164440"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLQ55_05400"
     CDS             complement(<1..418)
                     /locus_tag="PLQ55_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPK29401.1"
                     /translation="MAEKRYVFAFKCKEGEKLGRNYLGGKGANLAEMTRIGVPIPQGF
                     TVTTEACNLYYNEGKKLPQFVIDQIYNAVTELEKETGKTFGGKKNPLLVSVRSGARVS
                     MPGMMDTVLNLGLNDEVAEVLANETGNAAFAFDSYRR"
     gene            586..>1028
                     /locus_tag="PLQ55_05405"
     CDS             586..>1028
                     /locus_tag="PLQ55_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPK29402.1"
                     /translation="MEKKEKLVLAVDFGTQSLRVSIVNQFGEIKAIIKNKYNEAYFSS
                     EPSFAEQNPNYYWYELCNATYKLKEKHDDLLQKVLAMSVTTFRDSPVFLDKNYEPTRP
                     MVLWLDQRQAALHKKPPFLRRLIFAIVGMSETVTLNMKRTPAIWV"
BASE COUNT          349 a          195 c          175 g          309 t
ORIGIN      
        1 aacgacgata gctgtcaaac gcaaatgctg cgttaccagt ttcatttgct aaaacttcgg
       61 caacttcatc attgagtcct aagtttaaaa cagtgtccat catcccaggc attgatacac
      121 gagcaccaga acgaacactt acgagtagtg gatttttctt accaccaaat gttttaccag
      181 tttctttttc taattctgta actgcattgt aaatttgatc aatcacaaat tgtggtaatt
      241 ttttaccttc attgtaataa aggttgcaag cttcagttgt gacggtgaac ccttgtggaa
      301 taggaacgcc aatgcgtgtc atttcagcaa ggttagcacc tttaccgccc aagtaattcc
      361 ggcctaattt ttcgccttct ttacacttga acgcaaagac atatcttttt tctgccatat
      421 ttttcctcct tttaataagc agtatttaac ataccatcgc taattataat atcatagata
      481 ttaaggttta ttacaataaa tcactcaaaa acagcgaaaa ttgaagagaa aacactataa
      541 tagtaataat ttgctaaaat agtaatgcta aaggaaaaat cgaatatgga aaagaaagaa
      601 aaacttgtac tagcagtcga ctttggtacc caatctctaa gagtctcgat tgtaaaccaa
      661 tttggagaaa tcaaagcgat cattaaaaat aaatataacg aggcttattt tagtagtgag
      721 cctagttttg ctgagcaaaa tccaaactac tattggtatg aattatgtaa cgccacatat
      781 aaattaaaag aaaagcatga tgacctttta cagaaagtac tagcgatgag tgtcacgact
      841 ttccgtgata gcccagtatt tcttgataaa aactatgaac caactagacc aatggtgttg
      901 tggcttgatc aacgtcaagc agcgctccat aagaaaccac catttttaag acgcttaatt
      961 ttcgctattg ttgggatgag tgaaacggtc actttaaata tgaaacgcac accagcgata
     1021 tgggtgca
//
LOCUS       DAOPCD010000289         1027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_122077_length_1027_cov_1.936214, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000289 DAOPCD010000000
VERSION     DAOPCD010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122077_length_1027_cov_1.936214"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..>1027)
                     /locus_tag="PLQ55_05410"
     CDS             complement(36..>1027)
                     /locus_tag="PLQ55_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPK29403.1"
                     /translation="GLRRAIARTIIREQIRCGVGVIHLHRKINIAEVYLQKARVASEF
                     IVHENRIMAIYLYDDDMDKVVQREDYLRKELLDVSFEPGHEDKLRLVFQPIFNLQTGA
                     VSGFEALARFTSDKYGPISPLEFIPIVENNRIIMPFGKKIIDLAIQFIKDLSALGIDN
                     CPVSINISVSQLLDTNFAGDFFLRIESAGISPSLINVEVTETIFSTNHEQLNEALTEL
                     NEYGIETAIDDFGTGYSSLERLEKLHFTTVKMDRIFVETIDNKEKKACIVPEIINICK
                     KFNIKSLAEGIETEEQYNTLKFLGCDFGQGYYMSRPLEVKDAKAFIIKSRKAK"
BASE COUNT          331 a          190 c          166 g          340 t
ORIGIN      
        1 ttcgcggcta atatttatgg aatatgaaat aaatattatt ttgcttttcg gctcttaata
       61 ataaacgctt ttgcatcctt aacttcaagc ggcctactca tgtaataacc ttggccaaaa
      121 tcacaaccta agaattttaa agtgttatat tgttcttcgg tctcaatacc ttcggcaaga
      181 gatttaatat tgaacttttt acaaatatta ataatttcag gaacgataca agcctttttt
      241 tctttgttat caattgtttc aacaaaaata cgatccatct taactgtcgt aaagtgtaac
      301 ttttcaagtc tttctaacga cgagtagccg gtgccaaagt catcaatggc cgtttcgata
      361 ccatattcat ttaattcggt caatgcctca tttaattgtt catggtttgt cgagaaaatt
      421 gtttcagtaa cttcaacgtt gatgagtgag ggcgaaatac cagcgctttc aattcgtaag
      481 aaaaaatcac ctgcgaagtt tgtatctaaa agttgtgata ctgaaatatt aattgaaact
      541 ggacaattat caattcccag cgctgaaaga tctttgataa attgaattgc taaatcaatt
      601 attttcttgc caaaaggcat gatgatacga ttgttttcaa caatcggaat aaattcaagt
      661 ggcgaaatag gaccatattt atcactagtg aaacgcgcga gtgcttcaaa accactgact
      721 gcgccggttt gtaaattgaa aattggttga aaaacaagac gtaatttatc ttcgtgacct
      781 ggttcaaatg agacatcaag cagttctttt cgtaaatagt cttcccgttg aacaacttta
      841 tccatatcgt catcatataa atatatcgcc attattctat tttcatgaac aataaattca
      901 ctagcgactc gggctttttg taaataaact tcagcgatat taattttacg atgtaaatgg
      961 ataaccccaa caccacaacg gatttgttca cgaataatag tacgtgcaat tgctctccgc
     1021 agtccag
//
LOCUS       DAOPCD010000290         1019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_123671_length_1019_cov_1.821577, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000290 DAOPCD010000000
VERSION     DAOPCD010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123671_length_1019_cov_1.821577"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..611)
                     /locus_tag="PLQ55_05415"
     CDS             complement(78..611)
                     /locus_tag="PLQ55_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HPK29404.1"
                     /translation="MSINGKWIKIQSYKHDGRLHRFWRKSYVLEDNENWLVVASRRTQ
                     VIEGNGRKWFTKEPAVTFFNKKDWFNVIAMLKKEGIVYYTNIASPTLIDYGMAKYIDY
                     DLDVKKFADGTIKNLDKNEFLRNIENYQYSNELSTIVEYKQTQVVDMMNNKMFPFCDE
                     EVKKLYNRFIEETKVKQ"
     gene            complement(668..>1019)
                     /locus_tag="PLQ55_05420"
     CDS             complement(668..>1019)
                     /locus_tag="PLQ55_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29405.1"
                     /translation="VVIYIKPREESYQGSNGPDDVEDLELLVDTAEYRLYASNKELKK
                     AVSTKALNALLKVRPDKELADVSVSLYQNKFAVALTYSDALMVVPYKEEVPVHAIKKY
                     QEHLTNLNKFIALL"
BASE COUNT          316 a          177 c          144 g          382 t
ORIGIN      
        1 tttctaaaaa tacaattatt tagtaataat ttatgttttc taacattaaa cactttaaca
       61 agaaataatt actaaaacta ttgtttaacc tttgtttctt caatgaagcg gttataaagt
      121 ttttttactt cttcatcaca aaatggaaac atcttattat tcatcatatc cacgacttgt
      181 gtttgtttat attctacaat cgtcgataat tcatttgaat attgatagtt ttcaatgttg
      241 cgaagaaatt cgtttttatc gagattttta atcgtaccat cagcgaactt ctttacatct
      301 agatcgtaat caatgtattt tgccatacca taatctataa gtgttggcga tgcaatgttt
      361 gtgtaataaa caattccttc tttttttaac atggcaataa cattaaacca gtctttttta
      421 ttaaaaaaag taaccgctgg ttctttggtg aaccatttac gaccgtttcc ttcaataact
      481 tgcgtgcgac gcgatgccac tactaaccaa ttttcattat cttctaaaac atatgactta
      541 cgccaaaaac ggtgtaaacg accatcgtgt ttatagcttt ggatcttaat ccactttccg
      601 ttaatactca taaaaaactc aactctcgat tgttagttag attttaacat aaaaaagcga
      661 gtttttacta aagtaaagcg ataaatttat taaggtttgt taaatgttct tgatatttct
      721 tgatagcgtg tactggaact tcttctttat aaggaacaac cattagggca tcactgtaag
      781 taagtgccac tgcaaattta ttttgatata aggaaacgct gacatccgcg agttctttat
      841 cagggcgcac ttttaagagc gcgttaagtg cttttgttga tacagctttc ttaagttctt
      901 tatttgatgc atataatctg tattcagcag tatcgactaa taattctaaa tcttcaacat
      961 catctggtcc attgctacct tgataactct cttcgcgtgg tttaatgtag attacaaca
//
LOCUS       DAOPCD010000291         1014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_124602_length_1014_cov_2.332638, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000291 DAOPCD010000000
VERSION     DAOPCD010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124602_length_1014_cov_2.332638"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLQ55_05425"
     CDS             <1..290
                     /locus_tag="PLQ55_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPK29406.1"
                     /translation="STPTITVKGLEEDLTSVKPEINVIGMRVEEALNVIGPYLDRALL
                     KRYPSVRIIHGFGTGALRRGIHDYLAKLSFVKSYTLAGEYEGQGGATIVTF"
     gene            291..>1014
                     /locus_tag="PLQ55_05430"
     CDS             291..>1014
                     /locus_tag="PLQ55_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPK29407.1"
                     /translation="MKNKKLQFLIDHLPSDPGVYLMLDSHGRIIYIGKAKDLKSRVSQ
                     YFTRPQPPKVAAMVKRTAKFETIVTNSEKEAFILELKLIQTHYPRYNIMLKDDSHYPY
                     IALTKKGDPVVSIKRKTSDDKYIYFGPFPASQKAYQIVDLINKIFMTRKCKGKSKDPC
                     FYFHLGQCPGYCFKDISDLEKDKVRSDILTFLNGKDKDTLKRYETKMKIASAELDFEK
                     ARDYKEIVDAILHIYSSQNIELK"
BASE COUNT          356 a          174 c          204 g          280 t
ORIGIN      
        1 aaagcacacc aacaattacg gttaaaggtt tagaagaaga cttaactagt gtcaaacccg
       61 aaattaatgt gattgggatg cgtgttgaag aagcgcttaa tgttattgga ccctaccttg
      121 accgcgctct acttaaacgc tatccaagtg tccggattat tcacggcttt ggtaccgggg
      181 cactgcggcg cggtattcat gactacttag cgaaactttc atttgtaaaa agttacactc
      241 tcgccggaga atacgaaggc caaggagggg cgacgatcgt caccttctaa atgaaaaata
      301 agaagttaca atttttaatt gaccatttac cgagtgaccc tggtgtgtat ttaatgctag
      361 atagtcacgg tcgcattatt tatattggca aggcaaaaga cttaaaaagt agagtaagtc
      421 aatattttac gcgtccccaa ccaccaaaag tcgcggcaat ggtaaagcgc accgctaaat
      481 ttgaaacaat tgtgacaaac agtgaaaaag aagcatttat tcttgagctt aaattaattc
      541 aaacgcatta tccgcgctat aacattatgc ttaaagatga ttcgcattac ccatatattg
      601 cacttactaa aaagggtgat ccggtcgtta gtataaaacg caaaacaagt gacgataaat
      661 atatttattt tgggccgttt cccgcgagtc aaaaggcgta tcaaatcgtt gatttaatta
      721 ataaaatctt tatgacacgg aagtgtaaag gtaaaagtaa agatccgtgc ttttactttc
      781 atttagggca gtgtccgggg tattgcttta aagatataag tgacttagaa aaagataaag
      841 tacgaagtga tattctcact tttttaaatg gtaaagataa agatacatta aaacgctatg
      901 aaacaaagat gaaaatagca agtgcggaac ttgactttga aaaagcgcgt gattataaag
      961 aaatagttga cgcgatactc catatttata gcagtcaaaa tattgaacta aaaa
//
LOCUS       DAOPCD010000292         1009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_125517_length_1009_cov_5.081761, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000292 DAOPCD010000000
VERSION     DAOPCD010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125517_length_1009_cov_5.081761"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..616)
                     /locus_tag="PLQ55_05435"
     CDS             complement(206..616)
                     /locus_tag="PLQ55_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK29408.1"
                     /translation="MCALDGVVDCFNISFYNQRTLPKQADFKSDLTLHALIHYKQLAT
                     RDELDRFIDGHQENGHLKFSTLLPCNEWATKHQRVEYIDDLECEWVVLFNELLGQIYR
                     GAIIKRYDRIINKRARQSFKAHVDGQITQSWNRN"
BASE COUNT          268 a          196 c          216 g          329 t
ORIGIN      
        1 ttgataaagg caagtcaaat cttcaattcg cggcaaattt cttttgtgcc gccccaaagt
       61 gtgtagtttg acccttcaat tttcttttat ggtaataaac cgcactacga gaacatccag
      121 tttccttaat gatacgtgcc ggcgaaacac cctgcgcgat taactcctct accttagaat
      181 taaatttatt gttcatgaaa ttaaattaat ttcgattcca tgattgagtg atttgaccgt
      241 caacgtgtgc cttaaaagat tgacgcgccc gtttatttat aatgcggtca tatcgtttaa
      301 ttatcgcgcc gcgataaatc tggcctagta actcgttgaa aagcactacc cattcgcatt
      361 cgaggtcgtc aatgtattct actcgttggt gtttagtcgc ccattcatta catggcagta
      421 atgtcgaaaa tttaagatgt ccattttctt ggtgtccatc aatgaagcga tctagttcgt
      481 cacgcgttgc tagttgttta tagtggataa gcgcgtgaag tgtcagatcg gatttgaaat
      541 cggcttgttt ggggagcgtt cgctgattgt aaaaggagat gttgaagcag tccaccactc
      601 catctagcgc gcacacagtt tccggcttag tatagttcgt agtcattatc actcggtggc
      661 ctgcgtcttt caccatcgcg gcgattattt ccacggcggg gtgtagcgtc ggttccccac
      721 caattagcag aatgtctttg atttggtgat cacgcgcaaa tttcaaagcg ttacgcacat
      781 tatctagtga tataatttca ccacttccac gataagtatc tacgcaaaaa gggcaacgtt
      841 tattacattc gtgtgttagc actaaactca tgtaatcatt tgttttttga tcaattgaaa
      901 acataaaaat tataaatttg ttggattttt agatgtttat cggggataga tgtgaagtgt
      961 gtagtttgaa cgggaaaagt caagaagaat gtgtagtttg atgtgtagt
//
LOCUS       DAOPCD010000293         1007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_125918_length_1007_cov_3.292017, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000293 DAOPCD010000000
VERSION     DAOPCD010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125918_length_1007_cov_3.292017"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>1007
                     /locus_tag="PLQ55_05440"
     CDS             21..>1007
                     /locus_tag="PLQ55_05440"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPK29409.1"
                     /translation="MMGITAKVAFSFENKSGFSYMYQREYVIYYQAIMLFISYLVLRL
                     ARNTKHEYYLRNAFVFFEIAVMGTITMIGHGFISYKDSVSGGYQNYKDDLTTIREIKA
                     LDGSYYRIFNTRAYRGNDNLPMRENYNGLSAFHSLYNFELMPFNKWSKINYNHKGWSL
                     GVFEKRSMLNDFLQVKYYMLNNTNHVIYWSNAAPSVAPYRNVPNTYEFMEELSTDKRF
                     VYKMKEGFTLGFGVDNVISYENRLSDGTYFDLIEKDRALSPIQNEELYLTSALLSYED
                     HYEVTRTYSDLVSHDINAFPHQHYTRLSSTNGGLKVKYYEFTGRRYPYEAQTR"
BASE COUNT          329 a          157 c          197 g          324 t
ORIGIN      
        1 gtattatctt taacttaatt atgatgggaa taaccgctaa agtagcgttc tcttttgaaa
       61 ataagagcgg ctttagttat atgtatcaaa gggaatatgt aatttactac caagcaataa
      121 tgctttttat tagttattta gtccttcgtt tagcgcgtaa cacaaaacac gaatattatt
      181 tacgcaatgc ttttgtgttt tttgaaatag cggtaatggg gacgataaca atgattgggc
      241 acggctttat tagctataaa gatagtgtgt caggtggtta ccaaaattat aaagatgatt
      301 taacaacgat tagagaaatt aaagccttag atggttctta ttaccgaatt tttaacactc
      361 gtgcctatcg tggtaatgat aatttaccaa tgcgtgagaa ttataacggg ttaagtgcct
      421 tccatagtct ttataacttt gaactgatgc catttaacaa atggtcaaaa attaactata
      481 accataaggg ctggagttta ggtgtctttg aaaaacgtag tatgctaaat gacttcttac
      541 aagttaagta ctatatgctt aataatacga accatgttat atattggagt aatgctgcgc
      601 caagcgtagc gccttaccgt aatgttccaa acacttatga atttatggaa gagttaagca
      661 ctgataaacg ctttgtctac aagatgaaag aaggttttac gcttggtttt ggagttgata
      721 atgttataag ttatgaaaat aggctgagtg acggtactta ttttgactta attgaaaaag
      781 atcgcgccct ttcccctatt caaaatgaag agctctattt aacaagtgcg cttcttagtt
      841 acgaagatca ttatgaagta acaaggactt atagtgattt agtgagtcat gatattaacg
      901 ctttcccgca tcaacattac acgcggctta gtagcacaaa cgggggctta aaggtaaagt
      961 attacgaatt tactggccgc cgctatccct atgaagcaca aacaaga
//
LOCUS       DAOPCD010000294         1006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_3468
            NODE_126231_length_1006_cov_1.440589, whole genome shotgun
            sequence.
ACCESSION   DAOPCD010000294 DAOPCD010000000
VERSION     DAOPCD010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865203
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:54:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,064
            Genes (coding)                    :: 1,051
            CDSs (with protein)               :: 1,051
            Genes (RNA)                       :: 25
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 3 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126231_length_1006_cov_1.440589"
                     /isolate="AMR_MDS_3468"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1006)
                     /locus_tag="PLQ55_05445"
     CDS             complement(<1..>1006)
                     /locus_tag="PLQ55_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPK29410.1"
                     /translation="MIITSVIGSLQAFDQMYLMTGGGPEGSTTTFMLTVYKTMFEYEE
                     AGVASAMSYFLFFIIIAITIIQSKIVPQGYQTELQRGGDRPVKEVNEEVSKIGRKDDF
                     VPTLLVIKSEESSAERLQELLEKRKRENKRKIILHKVGVIFKYIILTLLAFAFIFPYL
                     WLISNSFKEPAAIFSAEQFSLIPRDVNGKIKLVFSNYKNAIEHLNLGQVFLNTLIVCG
                     VNTAVNLFLNALAGYAFARLKFKGRDFTFRVMILSIMIPGTIMTIPNLLICRLLKIDD
                     TLFVLILPFIMSVYNVFLMRQQFYNLPDELEEAAILDGAGPFKIFFKISLPLVTPMMV
                     V"
BASE COUNT          346 a          194 c          168 g          298 t
ORIGIN      
        1 cacaaccatc attggggtga caagtggcaa actgatttta aaaaatattt taaagggtcc
       61 agcgccatct aatatcgccg cttcttctaa ttcatcaggc aagttataaa attgttgccg
      121 cattaagaag acattgtaaa cactcatgat aaaaggcaaa attaaaacaa acagtgtgtc
      181 atcaattttt aataaccgac aaataagtaa gttcgggatt gtcataattg taccaggaat
      241 cataattgat aaaatcatca cgcggaaagt aaagtcgcgc cctttaaact tgagacgggc
      301 aaatgcataa cccgcaaggg cgtttaaaaa taagttcaca gcggtattaa ctccacacac
      361 aattagtgta tttaaaaata cctgtcctaa attaaggtgt tcaatcgcat ttttatagtt
      421 actaaataca agtttaattt tcccatttac atcacgagga ataagggaga actgctccgc
      481 actaaagatt gccgctggtt ctttaaatga attagaaata agccataaat aagggaaaat
      541 aaaagcaaaa gcaagaagag taagtattat atacttaaag ataacaccca ctttgtgtaa
      601 tataattttg cgtttattct cccgttttcg tttttcaagc agctcttgca gacgttccgc
      661 ggatgattct tcacttttaa taacgagcag agtgggcaca aaatcatcct ttcgtccaat
      721 tttactaact tcttcattaa cttctttaac tggtcggtcc ccaccacgtt gaagttctgt
      781 ttgataacct tgcggcacaa ttttagattg aatgatggta atcgcaataa tgataaagaa
      841 taagaaataa ctcatcgctg acgcaactcc ggcttcttca tattcaaaca ttgttttata
      901 aaccgtaagc ataaaagtag ttgtgctgcc ttctggcccg cctcctgtca ttaaatacat
      961 ttgatcaaaa gcttgaagtg aaccaatgac actcgtaata atcata
//
