LOCUS       DAOPCH010000001        51518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_766_length_51518_cov_5.707654, whole genome shotgun sequence.
ACCESSION   DAOPCH010000001 DAOPCH010000000
VERSION     DAOPCH010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 51518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51518
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_766_length_51518_cov_5.707654"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLU55_00005"
     CDS             complement(<1..237)
                     /locus_tag="PLU55_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86478.1"
                     /translation="MELVALLSSGKGTWGQVSGLMKHGEWDKIIILGDEFGAGFTHEK
                     KFEFIKVDLKSKIKDLRDEFLKKLKGKIDGTEVAL"
     gene            complement(239..496)
                     /locus_tag="PLU55_00010"
     CDS             complement(239..496)
                     /locus_tag="PLU55_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86479.1"
                     /translation="MDEYYGLRYKSSPEKVSYVLHKSEGNLDILGSLLSLQKMGIKLD
                     DNGDLSVPKCFLPPVQITKTSALEILTKNNALSIERLVEKL"
     gene            670..1302
                     /locus_tag="PLU55_00015"
     CDS             670..1302
                     /locus_tag="PLU55_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86480.1"
                     /translation="MAELEVELKKGLSELRKTKERKFDQTVDLIVNLQKFDVKKNNLN
                     LFVSVPHKVKDKKIAGFLEIKNKDVETITPDEFKKYSDKAKMKHLVSTYDFFIAQASL
                     MPSVATNFGRVLGPTGKMPSPQLGIILNADEKTINELKNKINTSIKIKVKEPSIKIPI
                     GKQSMKDEDIIENVMSVYNLLLKTLPRNKENIKNIEIKFTMTKPYKIYLR"
     gene            1304..2131
                     /gene="rplJ"
                     /locus_tag="PLU55_00020"
     CDS             1304..2131
                     /gene="rplJ"
                     /locus_tag="PLU55_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPJ86481.1"
                     /translation="MKSKTTTKTKTSKIPEKKKKAVSELSKLIQEKKTILVVSIKNIP
                     GSQFQEIVKKLRGKAIVKVPKKNLIIKALDSSKEEEIKKVKESITQDFALLFSDLDSF
                     ELAIDLVRKKSPSKAKAGQIAPEDIEIPAGPTDLVPGPAISELGALGIQIQIEGGKIT
                     IKAPKVVAKKGEKISQGAADMMGKLDIKPFSIGFIPVAAFDNTLKKFYPNIDIDPDKT
                     LEEFKEAYSKALPFAVNIGYATPETITFLIGKAGMHEKAINALLSQTPEINSTGEGN"
     gene            2133..2420
                     /gene="rpl12p"
                     /locus_tag="PLU55_00025"
     CDS             2133..2420
                     /gene="rpl12p"
                     /locus_tag="PLU55_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981366.1"
                     /note="similar to eukaryotic L12; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HPJ86482.1"
                     /translation="MEYIYAALLLHKLGKEVSEANIKSVVAATGAHADDSKIKSLVAS
                     LNGVDIANELANASLVAAAPSSSAPAEAKKEEAPKEEKHEAAAEGLSALFG"
     gene            complement(2537..3187)
                     /locus_tag="PLU55_00030"
     CDS             complement(2537..3187)
                     /locus_tag="PLU55_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPJ86483.1"
                     /translation="MIILDIETTGIDTGRCGIWQIASIDLDNPKNYFLEEGRIDNEDI
                     VAEGALKVTGKTEKYLRNTKKQPQKQLVSNYLEWRKSCKEKINIGQNVGWDFNFIQNK
                     CIKYGIMDKMCEVMGQRAIELHTLAQEKYREINGEYMKDEKGKSSMNLTKILKFCGIP
                     DERINVKGNEIIKEGKYHTALEDCVLEGEAVYRLKFGKNLFKEFKKFSIPDYLNRE"
     gene            complement(3237..4484)
                     /locus_tag="PLU55_00035"
     CDS             complement(3237..4484)
                     /locus_tag="PLU55_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPJ86484.1"
                     /translation="MMKGVFIILDGLGDLPHKMLEDMTPLEAAQTPNLDFLASRGQLG
                     YVHTVKPGFVPESDEAIVSLFGNELISSTRGQLEAIGSNLKLTRGDLAFRVNFGTIDS
                     LEKGNILDRRAGRTLTNSEANQLAKSLNKIKMPCKFVFKPTIQHRASLVFRGGFSDNV
                     SGNDSTYMDGMDLISNRIVKCKALDEEENSQYTANIVNEFLEKAFSILNTHPVNVERV
                     RRGLLPANFLLIRGAGIEPPKLKQYKNWVSASYMPLEIGFSNACGMKNYSFDYPELTG
                     IDAYENLWKGLRKACKHSVNVIKKNLKRADYAYIHIKETDLPGHDNKPIEKKLMIEYI
                     DITLFKYLRGVAPQNKIKVLVTGDHSTPCKLKGHSADPVPVLFYNCGKVPVAKKFCER
                     ECRKGELGEIMGPEILKKVGFVK"
     gene            4589..5830
                     /gene="ftsY"
                     /locus_tag="PLU55_00040"
     CDS             4589..5830
                     /gene="ftsY"
                     /locus_tag="PLU55_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPJ86485.1"
                     /translation="MFGKLKEKLKNWTQGLIKKTVEEEFEEEIKKTKEKEKKPKKQSK
                     EKFQEEKKEKKKLKKQEKEAEEIIQEIEQKEKAKEKIKPIEEHEYVKPSPKTFDKEER
                     AISEKIIQDIEAEEQEKKGFFKKVFSGISKVKISEEEFESYKEDLEMILLENNVALEV
                     SDKIIQQLKERIVGKEFLKKEIEGQIKESLKDIIYEILLEPFDLIEKIKDKKLSEKKP
                     YVILFCGINGTGKTTSIAKIGNLLKEKKISCVFAAGDTFRAASIEQLKKHGAKLNIGV
                     IAHEYGSDPASVGFDAIKYAEKNHIDVVLIDTAGRMHTEKNLMAQIEKIAKVCNPDTR
                     IFVGESITGNDIIEQIKTFNQSVKIDGIILSKADIDEKGGTALSVGYTTEKPILYLGT
                     GQEYKDLQKFDKNKFVDSLGL"
     gene            5974..7356
                     /locus_tag="PLU55_00045"
     CDS             5974..7356
                     /locus_tag="PLU55_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HPJ86486.1"
                     /translation="MLEKLGEALRKARDKLANALFLDKNLVESVVKELQRALIEADVN
                     IILVKDISDKIKKAAYDERIKEIEKKEHIIKLLHDELVKILGEYKTLELPSGKQSRIM
                     FLGLYGAGKTTTIAKLGNWYSKRGKKVIMVGLDVHRPAAKEQLEQLGKKNNIPCFVDF
                     DETDAVKTWKKFEKKHDLKKYDLILVDTAGRHTLDKELIKEIKELNKEIKTTETILVM
                     PADIGQAAKKQASEFQEALKISGVIITRMDSTAKGGGALTACAETKAGVYYITTGEKI
                     NDIEEFNPESFLSRLLGMGDLQALIEKIRSVTDEDQQEKMQKRLEEGKLSLEDVIEQV
                     KSMNQIGGFDKIKGMIPGLGKAKIPEGMLESQQEKIAKWEHILKSMTKEERENPELFD
                     KQTSRIGRVAKGAGVNNSDIRALLKQYKMLQDLVKGGMTDMDMSKGFSQKQMQRMMKK
                     FGKKKMMRFK"
     gene            7411..7818
                     /locus_tag="PLU55_00050"
     CDS             7411..7818
                     /locus_tag="PLU55_00050"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HPJ86487.1"
                     /translation="MENKTKYTIILTIFLISLIFSGILTFVSLEEACGGIQTTCYAVQ
                     TSQYESTFGIKNSNIGLVAFSIMAILVFLHIKQPTKYKKQMIVAGIIGGTIFALYFLY
                     IQFFVIDALCKYCMVIDIGMLLNLGIIIFWKDK"
     gene            complement(7841..8515)
                     /locus_tag="PLU55_00055"
     CDS             complement(7841..8515)
                     /locus_tag="PLU55_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86488.1"
                     /translation="MRFKDIFLTGALTLSTILPSFSQDKLNYEVRTKGGGFPATTSFI
                     NLSEKEIDFSFLLGIYSMKFNQINDSTYNETKNFMFKKEFFDYAKGSGYILLDYSVEM
                     GRLRNVKEKLINQKLDEKYKTFPEILKDFREDGFKDKDSLHFFLMGLPYSLKIDKIES
                     GDTTFYSSGLNEAIKFEPGDDFHFSYPIEMTVVEKEGKRVIIGFSTRFLNVKKRKEGS
                     LEGRLK"
     gene            8604..9212
                     /locus_tag="PLU55_00060"
     CDS             8604..9212
                     /locus_tag="PLU55_00060"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF011462.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex kinase/ATPase Bud32"
                     /protein_id="HPJ86489.1"
                     /translation="MKLIQQGAEAKIFLDEKKNQIVKERISKSYRIPEIDKKIIKQRT
                     KAETKLIEKANKTIHNFSPEISREENKIRMPYIDGEKLSEALNSFPLEKQKRILNKIG
                     KSISKLHKSDIIHGDLTTSNMILKDNKIFLIDFGLGYISKKIEDKAVDLHLLKQALEA
                     KHFQHWKELFEEFKKAYSKDYPESKQIFERIISVERRGRYRH"
     gene            9342..10196
                     /locus_tag="PLU55_00065"
     CDS             9342..10196
                     /locus_tag="PLU55_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPJ86490.1"
                     /translation="MFCSNVEEIKSIREIFDIHPAAEEDILSNQTRTKYEEFEENTAV
                     IMQGIKEIEEFNIKIYNLSFIFGENYLITSHFENNEAIDSLISNPKKLENLMKKGAGH
                     IFHYLLDKEIDKCMQIKSILLEEFKQTEKEFIKNPEKEVLEDLFQKELTLLETRQVME
                     SLTDLCLSLTRPTDNYLDNELLPYFRDIYDHSFRTTESLKSMLGRINGMRNAYQTITS
                     NRLNETMKILTMIMAIMMPITIVTGFYGMNVRLPLEDNYYSWMWILAIMIFVSLIMYL
                     IFRRIKIN"
     gene            complement(10217..10308)
                     /locus_tag="PLU55_00070"
     tRNA            complement(join(10217..10251,10271..10308))
                     /locus_tag="PLU55_00070"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10272..10274),aa:Gln,seq:ttg)
     gene            10506..10847
                     /locus_tag="PLU55_00075"
     CDS             10506..10847
                     /locus_tag="PLU55_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86491.1"
                     /translation="MSTEKDEKPKKEQGKMSLEEYEKEVIKLADKGLTSEKIGEELKR
                     RGIHPKEYNKKISKILKEKYINPDLKNVEEKLEKIEKHLKNNKQDKRAMRERSRVFSQ
                     VRKIKKYQKKL"
     gene            10864..12201
                     /locus_tag="PLU55_00080"
     CDS             10864..12201
                     /locus_tag="PLU55_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPJ86492.1"
                     /translation="MKKEDEIFEKELKSFAEEFLKKTENKEILIMSHFDTDGITSASI
                     IISTLKKLDRKFSIRILKNLEEKTIKGLPKDKIIFFLDLASGSLEHIKNNNLKDVFII
                     DHHEISQKIPENVRIINPHLTNKQKISTSGLAYLFAKQIIPEAKELSKLAILGMIGDC
                     LEKEIDSLNHGILEESGVQRKRGLLIYPSTRPLNRVLEYSSNPYIPEVTGNLKGVLEI
                     LRESGIMPERGKYKSIMELDEKEMKNLTTSILLRNPKIKSKDLVGDIFLIKMFNKIED
                     ARDLSAIINACSRSGRPELAIQYCLEIPKSKKEAELAHIKYKQNIITGLNFVQETKKI
                     QGKGFVIINAQDNVKDTMIGTIASIISNSSIYEEGTIITTMAYYEDKIKVSSRIVGRK
                     GRNLRELLASVTNLTGGESGGHEHAAGCLISRKKEREFIDILKKNLEIELIKI"
     gene            12249..12956
                     /locus_tag="PLU55_00085"
     CDS             12249..12956
                     /locus_tag="PLU55_00085"
                     /inference="COORDINATES: protein motif:HMM:NF019179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86493.1"
                     /translation="MVFEEGEMVLCTVDRIAGTVVFVKIEGEQREGSIILSEIAPGRI
                     RNLREYVIPKKKIVCKVLKDTGGTISLSLRRVTPKEKKEVMDQHNQENSYINILKSVL
                     KEKSEEVIEEISKRGRIYEFFEEARENPKILEKLVGKENSERVLDIILEQKKKRIIIK
                     RIVYLKTLDSEGISHLKKVLDTPKEIEVKYISAGKYSLKIETEDGRKGEQKMKNTLEE
                     IEKKAKEKGMIFEIKEK"
     gene            12958..13347
                     /locus_tag="PLU55_00090"
     CDS             12958..13347
                     /locus_tag="PLU55_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPJ86494.1"
                     /translation="MADKFNVKIIGILFNPPARKILLGRNIGERNLSFLEGDLKYEEE
                     LDVGLKRVAKEKTGYIIHNLGAVYAENRLKDKQALRLYFLCEATEGKETPGKNVAELK
                     WISPKEVEKQLKKKLPTRLKGYILGLG"
     gene            13344..13517
                     /locus_tag="PLU55_00095"
     CDS             13344..13517
                     /locus_tag="PLU55_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86495.1"
                     /translation="MKLKFYINSNKEKIYTLDEEIQGQKTQDAHYKFVKIKDASHSNR
                     LKIQKNTKTYSKR"
     gene            13553..13885
                     /locus_tag="PLU55_00100"
     CDS             13553..13885
                     /locus_tag="PLU55_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86496.1"
                     /translation="MKKREVYEKKYRGKQVEDYYKLISNIREISFEEPTGINSNSISN
                     TWGEKGYISFDGDFLVRVSKNQLNQSSKLINKAKITVEKIGPDISKESKIEKMLLERG
                     FKKIIERK"
     gene            complement(13888..14253)
                     /locus_tag="PLU55_00105"
     CDS             complement(13888..14253)
                     /locus_tag="PLU55_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86497.1"
                     /translation="MKNKISRNYEEELSFAVVDKNLLEGKSLPVYTIFHLKKNPFKGI
                     ENEVLIKDLEYKVFAGYIKGACFSKLDTEMVLYNGFSKKLKKSFYPETSIFPLNDSEM
                     KNLKEYMDLPKHIVIKKAK"
     gene            complement(14295..15512)
                     /locus_tag="PLU55_00110"
     CDS             complement(14295..15512)
                     /locus_tag="PLU55_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HPJ86498.1"
                     /translation="MEKTGVFADLHIHSRFSRACSKDITLENLEKWARVKGIGLLGTG
                     DFTHPIWLNELKERLTERDGILYSKSGFKFLLTGEVSLMYTQGRGRRVHLVLLVPNFE
                     IADKINAYLDKKGRRDYDGRPIFKISCEQFVKDMREISKDIEVIPAHVWTPYFGVFGS
                     KSGFDSLKEAFGEEFENIHAIETGMSSNPEMNWHLSELNNKAIVSFSDSHSFWPWRIG
                     REATIFEKADSFKDIIDGIRNNSILGTIETDPGYGIYHYDGHRDCGFSSSPEETKKLN
                     GICPVCKHPLTIGVANRALELKDQIKEENRKRKKYYTLLPLHELIASSIGSSIESKKT
                     WEVYNMLIEKFENEFNILLNVYKEDLIKELKDNEKLVKLILENREGKIKVKPGYDGKY
                     GELVDGEKQATLF"
     gene            15566..16030
                     /locus_tag="PLU55_00115"
     CDS             15566..16030
                     /locus_tag="PLU55_00115"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPJ86499.1"
                     /translation="MTEENKKERALILIKPDAIARKLSGIVIYDLDHLNLKMIGLKLV
                     SVQKHLAEKHYAVHKEKPFYNDLLKYLMGEYHNVHAIIAIAYEGENAIQKLREYAGKT
                     NPDDAEPSTLRGRYGKVNSKTNNFDNVMHISDSPENGEKEISIWFSKEELVD"
     gene            complement(16066..17088)
                     /gene="radA"
                     /locus_tag="PLU55_00120"
     CDS             complement(16066..17088)
                     /gene="radA"
                     /locus_tag="PLU55_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014122817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HPJ86500.1"
                     /translation="MAAKKEDIEEEKEEKEEKKSKKKETKEPTLMDLPGIGPAIAAKL
                     EGAGIFDLMSLAVSSPATISDISGVSPAVSRKAIQAAREMLDLGFQDGVEYAQRRSNV
                     SYITTGSKNFDELLGGRGIESRAITEAFGAYGSGKTQLGLTLAVRVQLPVEQGGANGK
                     CVFIDTEGTFRPVRIRQISEGLGANPEKVLKNIFVARAFNSDHQMLLLEKVTEMVKAG
                     EPIKLLIVDSLTAHFRAEFSGRGQLADRQQKLNRYMHDLMKLAETHNLAVYVTNQVMA
                     NPAQMFGDPTTAIGGNIVGHASTYRLYLRRGKQGSRVAKLIDSPNLPDNETVYYVTAG
                     GISDKE"
     gene            complement(17189..18118)
                     /locus_tag="PLU55_00125"
     CDS             complement(17189..18118)
                     /locus_tag="PLU55_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPJ86501.1"
                     /translation="MVLGNYGRIVEKIAKHSGLSVDEVNRKIEAKRAKLSGLISQEGA
                     AQIVAAELGINFENEKLKIEELLPGMRKVNISGKIIRLFPVRSFKTQKGEDGKVLNLV
                     LADETSNIKVVIWDTNHIALIEKGEITEGDVVDISNGSMRENEMHLGSFSEFKRSSKE
                     MGEVMTDRIIREKKISEFKLGDSISVRAFIVQTFQPRFFEVSKETGKKLTESEREKGV
                     ATDRKALINIVIDDGTETIRAVIFNDNLSVLGLNDLENPERLSYQIENLLGREMIFSG
                     NVRKNNFFNNEEFIVDGVKEVNLDELISQLEKK"
     gene            18217..18489
                     /gene="ef1B"
                     /locus_tag="PLU55_00130"
     CDS             18217..18489
                     /gene="ef1B"
                     /locus_tag="PLU55_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012939.2"
                     /note="EF-1-alpha; functions during elongation stage of
                     protein translation; forms a dimer; associates with
                     EF-1-beta-GDP complex and promotes exchange of GDP to GTP
                     resulting in regeneration of the active form of
                     EF-1-alpha; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HPJ86502.1"
                     /translation="MVGINGVKYKLMPESTEVDLKEMENAAKKIVEEFGGTNKEYSIE
                     NIAFGLRALIVFFFYHDDKNIDVVEERLSKIPNVASVQLIDMRKIA"
     gene            18529..19170
                     /locus_tag="PLU55_00135"
     CDS             18529..19170
                     /locus_tag="PLU55_00135"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPJ86503.1"
                     /translation="MPLSKEEIENFKKKIILNIETNFPEERKAAAVEKIKSMNDNEFI
                     EFLKNNDLIESEKEGKENRNNNSTPFRMIIEEKIPSYKIHENKEAVAVLEINPISEGH
                     TIVIPKKPIADSKKITKEIISLANKVAKTIKNKLKPKEVSISPSSVLGEIIINILPIY
                     TNETINSNKKQISQEKLEELKKILETKPKLKKEKKPTVKKINTSKIIIPRRIP"
     gene            complement(19167..19985)
                     /locus_tag="PLU55_00140"
     CDS             complement(19167..19985)
                     /locus_tag="PLU55_00140"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPJ86504.1"
                     /translation="MALKEVFLIVLCLIWIAFAVIQDLRKREIANWLNFSLIIFAIAF
                     RFFFSLFSEEGFGFFYQGLIGFAIFFVIGNMLYYGRVFAGGDAKLMIALGAIIPLSNN
                     FPENLNIMLKFLLLFLVSGALYGIIFGVALGLKDKKKFSKDFASRFKKHKKIFYISII
                     VSIVFLVLSFVESLFLYLGILFFISPYIYFFAKSIDESCMVKKVNTKELTEGDWLYKD
                     VKIGKRVIEAHWEGLSLSEIQFLRKNMKFVSIRQGIPFSPVFLISYLALIIFFI"
     gene            complement(20178..20513)
                     /locus_tag="PLU55_00145"
     CDS             complement(20178..20513)
                     /locus_tag="PLU55_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86505.1"
                     /translation="MINKKAQGLSTSTIILLVLGIIILVVLVLGFRSGWKPLSELMGG
                     KNNLDTIATSCNSACTTSSKYNYCSVMKEVKDGKNPKFEATCNDLATNPVYTSRNYGI
                     PTCPGLCTD"
     gene            complement(20572..20886)
                     /locus_tag="PLU55_00150"
     CDS             complement(20572..20886)
                     /locus_tag="PLU55_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86506.1"
                     /translation="MGLQKNSYSKNFKGDKKGDIPVTVLIIGVFGVCILAMVTFIASS
                     YRINISLIGVEIMEKANIQIESDNPSHLFLYKKVTKFSPEWGFNWFKEKVIFSVEYNP
                     RP"
     gene            complement(20888..21148)
                     /locus_tag="PLU55_00155"
     CDS             complement(20888..21148)
                     /locus_tag="PLU55_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86507.1"
                     /translation="MLNSKKAQVGETVTWIVATIVLIAILLIFIFASSILAKSKSLKV
                     NFKANSEEDVSWIDSKTQMAYSLNNANRNKIVTWISQKEDLG"
     gene            complement(21135..21674)
                     /locus_tag="PLU55_00160"
     CDS             complement(21135..21674)
                     /locus_tag="PLU55_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86508.1"
                     /translation="MDKRGTDKMLSIYWFLVLTLVAGGVFAMVYIFYGAPHDVREIES
                     ELLAQKLADCISKRGVIPSYFFVGERFNENLLKGFTERCNINFDVEEDYGDENEIQYF
                     YRVNFYLIRDLNNPVFSVYDGNLNWESECYIKKDNSKEYQRLAKCTESRFYAVDSKDN
                     QYLIKILSAIGKSKKNVKQ"
     gene            complement(21667..22083)
                     /locus_tag="PLU55_00165"
     CDS             complement(21667..22083)
                     /locus_tag="PLU55_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86509.1"
                     /translation="MKKRGAILVENVVFLILNLLFLSILVLFLVKQGSGATVLEEAYS
                     KQIAMVINSAKPGMVIRLDMDKGRKIAEEKGFDFGNSVIIENNVVYVQLTPDGRYSYA
                     FFNNVDVSSRALKDEKGKYSGTYLFTVKPKGGNLNG"
     gene            complement(22118..23041)
                     /locus_tag="PLU55_00170"
     CDS             complement(22118..23041)
                     /locus_tag="PLU55_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86510.1"
                     /translation="MRNIKGEITTQQIVLIVILLVSFIVILFLLFRLDLGKESDKEIC
                     HNSVVMRSASISKEAIPLKCSRTYICLSKDGSCERMSSPDVKKVKTEEEIYKVLAEEM
                     ADCWWMFGEGKLDYIGKDFFLRDNYCSICSQVGFDDSIKEINGVGETISKDKLYDYLS
                     KTKMPDSEVTYSEYLFGTNDINKLKSTALKVDNGREMVVGTFGTIDLSKQHFVLTGIT
                     SEVKGTGWKVVFGLGVAAVGLVTGYTWVGLISGALIVGAGVVGEGVTPEISAILVEGN
                     GVKNTFMAPTIQEANSNSLKKLNCYDILTST"
     gene            complement(23141..23656)
                     /locus_tag="PLU55_00175"
     CDS             complement(23141..23656)
                     /locus_tag="PLU55_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86511.1"
                     /translation="MVNLEKNKRGFLLAEETLKVIIAVICILFLVYILMSVYNSHNAD
                     KKIILAKEVLLGKEGESVPGIERIISSLDDGESEVKDISDPERWHIYSFVREEKPNSC
                     LDQKCLCICEASLIKKLNSQAKKCDEKGACLIVPNLASTDIDIKIKETRRNLVIEIKN
                     SGGRIFIEETR"
     gene            complement(23658..23822)
                     /locus_tag="PLU55_00180"
     CDS             complement(23658..23822)
                     /locus_tag="PLU55_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86512.1"
                     /translation="MELTIENLIKIVLGVLVVVAVSYAVYYFFSNNVFDFFKNFGANS
                     TSKFLASLII"
     gene            complement(23822..23980)
                     /locus_tag="PLU55_00185"
     CDS             complement(23822..23980)
                     /locus_tag="PLU55_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86513.1"
                     /translation="MKKRNKKGVISEYLPWILIALVVLTIVLISIFFLKNEGISLVDQ
                     IKNLFRRA"
     gene            complement(24012..26201)
                     /locus_tag="PLU55_00190"
     CDS             complement(24012..26201)
                     /locus_tag="PLU55_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86514.1"
                     /translation="MQPNKKPTVDEILKKYKDKIEGQIKTTDIGSFKESKSYTKFKEE
                     TQKELTTYERWCNTLGSIIKMNPSKKDEEKIRRDIEIAHMNIEPWQAITLSVVIFVLV
                     FFIGILFSVGYTFIGERTLADFPFIFFFLMMMIAMFLFYYFNGYPTRFANKWRLKASS
                     QMVPAILYVVVYMRHTPNLEKAIAFASEHLQYPLSLDFKKIFYNVEIGKYSTIKESLD
                     NYLEGWRDYSTEFIEAFHLIESSLYEPDRARRISTLEKAIQVVLDGVYTKMLKFTHDV
                     KAPLTNVYMLAIMLPILGITLIPLASAMVSGMVKWSHVFILYTIVIPFLAFFMLDKIL
                     LLRPGGYGETSYLEKNPLYPEFKSKGPYLYAFLICLPFLLLGLLPLIFQYTPLPDLLG
                     LEKDYSLADLGFSFEGAKDTMIFDFKKTEEGGLAGPFGGVALILSLFFPLGVALFFSI
                     AYKDRTANIILERNKTKQLENEFNNSLFQLGNRLGDGVPPELVFGKVANSSKGLVTGE
                     FFKRVNYNIQQMGMSVEKAIFDSRRGVILFYPSDLIATSMRVLVESSKKGLDIAAVSL
                     MSISEYVKNIQKITERLKDILAEIVSEMKSNMTFLAPLLSGIVVGLALMITSILNVLK
                     TYLDSSADVGSAVNMNILNIMRNFDVVSMIPPYYLQIAIGIYLIEVIFILTSTLVTID
                     SGEDKLERTNKIGQNLKKGIVFYFLTALLSSVALFGLATIVLQNVIG"
     gene            complement(26312..28846)
                     /locus_tag="PLU55_00195"
     CDS             complement(26312..28846)
                     /locus_tag="PLU55_00195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPJ86515.1"
                     /translation="MDKSLKNNFSFEDLLSLSTQLDKKGLFSLNSLLLKTVLSQDNIN
                     YFTFSKQALLPQDDIYKVDLKKPPINRIESNNSYFFNGGLFEKYYPLLLEKLKDSLYK
                     EGRYMESQGVNSVFNKEFNLSNDDKGLENKLYSDRKFVQENLYPSSSPESSYVLHFDN
                     NIENLKRTIDSITSFGKKKELILLVDNSKEVDSLRKEEFIKKNLLSIEDENSFQDIDS
                     KDNDSLNIRIVDLKGGFNKYTSYNLGALKSLSSNSKYVSFLKNGDVLDKGGFSNLISR
                     LEGGKLFAVGGISYFDTSGKKVDDVNLRWRDYLYKNYKRYHLDKFENYSLFDFSNHVI
                     SSKNLEELLLSDKFVFDPRLDKHSAENDFFRRFVNKYSDKGIDYLEEISAHKEKNLNF
                     NTKNGKKNILFITSTLENTHGVPVVVNNISNNLNKDKYNVFYFVNDFDSKKIRFFDSS
                     KKNFIEATSRKELFEILKTYNPSFFDDVNIIHSHTWHLADDFKHFHTKNIRTGVHEED
                     ETKFKDFLNYFSNAKFIFTDHSNPSEDLANINESYLKGKDYQKLSDLEKQKFLDNYKV
                     HSIDDKDWMNRWAFTSMSGKKQMAYLANLVINVSNSQHEEGKKIIPGYTFSNQKTVYN
                     GIDLIDITKDPSIDKEIGSLADSFKENYNLGKKDNVVLYAGRLSRRKGVFDLAESLKK
                     LRNKVGNLKLLVVGDARADIRDKLKELSGMNNIIYTGKIADRKELAALYRIADITVQP
                     THGECFNQVIGESLSQGTPVIASSVSGPKEVYVDNNWAYGVERTSDHQRNINNLSAAI
                     NDALSNKHILRYDVKKRIIPEIREHLGLAKMVREHEKIYDNLSSNK"
     gene            complement(28927..29340)
                     /locus_tag="PLU55_00200"
     CDS             complement(28927..29340)
                     /locus_tag="PLU55_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86516.1"
                     /translation="MPKHKRLNNLKYSVSLIPTSHNFSIDNSVGNISRLHFKRGISYF
                     DFKVFYNNDEFYGIKYHFKDGHYKKSYFFKEGDITINKKNILVNKQQDIKELNFFLIE
                     HFYNILENRYKLSKDSKLSFDYLTEDLRDILRFIS"
     gene            complement(29375..29896)
                     /locus_tag="PLU55_00205"
     CDS             complement(29375..29896)
                     /locus_tag="PLU55_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86517.1"
                     /translation="MYLGNLLGQWEAAGIFAYALPFLLIFAIVYAILSFVNVFKNNKA
                     VVTIISLAVALMALQFEIVSYFFADIFPRLGVALSIILVLLILGGMFLDWDNKGMKWF
                     LFAVVAIAIIIVVWGPLRNIGLFSGIGINIFGGNIGTIIAVVLIVGLIAWVISGGNKD
                     SHIRYPPERPYGY"
     gene            complement(29936..32224)
                     /locus_tag="PLU55_00210"
     CDS             complement(29936..32224)
                     /locus_tag="PLU55_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HPJ86518.1"
                     /translation="MQFGKEDELYSYEVKREGGEDVLYINYMGAPYVPSLAEHPEVME
                     RTIDSLIENPQVSRVVFVQQKNYNYDFKETGYLLEIAQFYVYLVKQEKILSQEKLITS
                     NPDFFPRRYNEMFSFLYLLKKDPLSAYFQLRKIILEGKIILDKLDGVLKLDQKNYLAL
                     VEKILENFSKTNFIQTFQQYSTQYKRGDREIYHKLFRPDVIANFTFTRLVSDLPRDAE
                     IISQYKISNGEDDESNVTILRKKNESKLIYHLVPPENTLTEDQSIILNLARGVLIEHQ
                     PKAEEFTDTERVRQVFMNISKDLIRDLSQSRDIKLDYSSLNKLSSILVRHTIGFGIVE
                     VLLQDKNLQDISLNAPIPQTPIFVRHQEYDQCTTNILPSQEDADSWAAKFRMISGRPL
                     DEANPILDTQLEIGTLRARISIVQQPLSCDGLAYSIRRHRESPWTLPLFIQNKMINPF
                     GAGLLSFLIDGARTLLVAGTRSSGKTSLLGSLMLEIVPKHRIIVIEDSLELPVEALRK
                     LNYDILRMKVRSALLKTTTEVSAEDGIRTSLRLGDSCLIIGEVRSVEAKALYEAMRVG
                     ALANVVAGTIHGASPYGVFDRVVNDLEVPITSFKATDSVIVCNPIKSPDGLHAWKRLI
                     QLSEVRKHWTKDPLAEKGFVDLMKYNIEKDELEPTEDLINGDSQIIKDVASNVKGWAG
                     NWDAVYDNILLRSKIKNELVNVAKKTSDYDLLESKFNTLSNHAFHRISDEIIQEVGLP
                     LGERVFPVWQEWLNKEIKKRII"
     gene            32302..32646
                     /locus_tag="PLU55_00215"
     CDS             32302..32646
                     /locus_tag="PLU55_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86519.1"
                     /translation="MAEEGYENQYFTEVSTKIRDLEDKQRIIKDRVLLIGQNLIEVKK
                     EMHSTFLNIKKEIENLKIDVERLKSFFETASGEFSKFAKKEELEILSKQAKMFQPLEF
                     VTRKDLENLKNK"
     gene            32661..33386
                     /locus_tag="PLU55_00220"
     CDS             32661..33386
                     /locus_tag="PLU55_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86520.1"
                     /translation="MGIIEDITNMKNQGMQENEIYRKLQEKGVSPKAVEDAFNQIKIK
                     NAVSAEYAGEEGMEPSIMKNELQPPTPQAPPLYAPKTQEIEHDPREFYSPQPMVPQFN
                     ENIQPPMYNAGPEYGQEQYIAQEGYVENPMGGGYDTDTIIEITEQVFSEKIKKEQKQI
                     ESLNEFATLVETRISNYNERIKRIESIIDKLQIAILEKIGSYGKNIESIKNEMGMMQE
                     SFEKMVPILHEKSEEKKTVKKRK"
     gene            complement(33431..33997)
                     /locus_tag="PLU55_00225"
     CDS             complement(33431..33997)
                     /locus_tag="PLU55_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86521.1"
                     /translation="MINEGIVLASYGFFGGGTIGDLLGQWEAAGIFAYALPFLLIFAI
                     VYAILSFVNVFKNNKAVVTIISLAVALMALQFEIVSYFFADIFPRLGVALSIILVLLI
                     LGGMFLDWDNKGMKWFLFAVVAIAVIAVVWGPLRNIGLFSGIGFNIFGGNIGTIIAVV
                     VIVGLIAWVINGNTKISVGDAPVRNPTR"
     gene            complement(34036..34485)
                     /locus_tag="PLU55_00230"
     CDS             complement(34036..34485)
                     /locus_tag="PLU55_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86522.1"
                     /translation="MFFILFAVLEKTKVFGSGTKQINALISFVISFIFVSAVFPKEVT
                     SNLILFLAIALVVIFVVLLLWGFIMGEEGLNVFKNAPKGLKWAIGIFVVITTLIAVLW
                     AAGVDTASFFDRLFNSSWSNQFWTNTVFIVLVVIALVVVLASGKSKG"
     gene            complement(34572..34835)
                     /locus_tag="PLU55_00235"
     CDS             complement(34572..34835)
                     /locus_tag="PLU55_00235"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HPJ86523.1"
                     /translation="MKIKKISEVLGKRVFTDSGEFFGEVEEANLFENKIDGWRIRVGG
                     NILSLMGGARGVIIPHQFVRSISDVFIISKSALPSQDTGFEEV"
     gene            34953..35606
                     /locus_tag="PLU55_00240"
     CDS             34953..35606
                     /locus_tag="PLU55_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86524.1"
                     /translation="MAKGKEIGYVILITIITAFAISLTKELSINLNLLLLSLLAVFIL
                     LTVNILIKKTAAYFLESEIEIKLWEIKRFGFKTGQHFKKAFPIGAFMPIIFKILLLPL
                     NSFVWMASLVFDVKAKSYRAAKRHGIYSFSEMTEEHIGKIAATGIIANLFLALIFYFI
                     GGNFGVLFAKLNIWFAFFNMIPISDLDGNKIFFGNLVLWSFLAIITLIAVGYTFFLI"
     gene            35656..37221
                     /locus_tag="PLU55_00245"
     CDS             35656..37221
                     /locus_tag="PLU55_00245"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPJ86525.1"
                     /translation="MEFLEKIKPRKYQQEIFETCKDKNCLVVLPTGLGKTLIALMLTI
                     ERMKLFPGEKVLFLAPTKPLAEQHLKYFKRNLPELFGEMQLFTGSVNTEKRKKIWQTA
                     DIIFSTPQCIANDLKKRMYSLKDVCLLIEDEAHRCIKNYDYRYIAEEYKNQSNHPRII
                     GLTASPGSEKSKIKEICKNLSIEEVELRTRDSSDVKEHLQELEFEKIMLDFPAEFQDM
                     RHILLKLFGEYVSELRERKVLFGPASKTDLINLQKKISSTISSNYKNFNYMRGASACA
                     QAIKIQHALELLETQTLEGFYKYLKGLFDQASKKQSKGVIKLVSKPEFNFIYSQANEL
                     LAKKVEHPKMQEIISIIKREREFNKNLKMIIFTQFRETAGNISRRINEIEGVKSKVFI
                     GQAKKDSLGGLNQKQQKKIIEEFSEGKINVLCATCIAEEGLDIPEVNIVVFYEPIPSA
                     IRTIQRAGRTARLMKGKLIILITKKTRDEAFFYVAKSREKKMKTAIESIKEDLSKEGP
                     IKTIKTEVQKKLE"
     gene            37218..37922
                     /locus_tag="PLU55_00250"
     CDS             37218..37922
                     /locus_tag="PLU55_00250"
                     /inference="COORDINATES: protein motif:HMM:NF014752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HPJ86526.1"
                     /translation="MKTVKSPIMLDIFSKRNKIKEKKQEDKAEKIIIDYREKNSLVAS
                     YIIKEGLEVEFKELKIGDYIVMDTIIERKTVSDFISSMINQRLLKQIEEIKQYENKLI
                     IVEGISEKELYGEENSGVNPNAIRGFLLSIALKHKIPLIFSKNSEDTAKFITILSKKK
                     DKEINLNAKKKTLGKKEQLQFIIEGFPGIGPKKARALLEKFGSIQNIILAPTEELREI
                     LGVKSEQIREIIERKY"
     gene            complement(37934..38749)
                     /locus_tag="PLU55_00255"
     CDS             complement(37934..38749)
                     /locus_tag="PLU55_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HPJ86527.1"
                     /translation="MFVVKVPGINGNSDTSGCEKSGNEILKILKKEGCVNESGNVVDI
                     TNLDLEEIHLDNSNLKLTNHLIYKNSFEIFESKPKTIFLGGDHSISYSLCKAFLDSSK
                     KRGKEPCLILFDAHPDCKPFLNSSQDFPKNEEWLRKLIEDGFPKKNILIVGIRNMSKE
                     EFKFLKDNSIKTLSLNSLLEDIEETCEIIMEFSDKKDLYLSVDIDVIDPLFAPATANP
                     EIGGLTSREFIYLISRLNKIKNLRAVDLVEINPLKDKEHLTLTLGAKIISELI"
     gene            complement(38792..39997)
                     /locus_tag="PLU55_00260"
     CDS             complement(38792..39997)
                     /locus_tag="PLU55_00260"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPJ86528.1"
                     /translation="MKVSFYRKVLKNGMTVILEKRNLPIVSVAFAVRNGGVNEKEEEK
                     GISHFIEHMLYKGTPSRNAKKIAEEIEKKGGDLNGFTSDEITAYWCKMPSKNLKCALD
                     VLSDMVKNPLFDEKELEKERKVIFEEIKMYHDNPRLHSMDEIQKLMYNGTMKINLAGT
                     FETMNSISREKMVKKFKEVYTSNNLILCVVGDADFKELVKFAERNFDNSNSKIKVEKF
                     GLERGIRTEKRVGIDQANVVFAYHVPLAGDKKSYSAIVLNALMAGGMSSRLFSEIREK
                     RNLAYAVKGDVNINKTFAYNIIYVGTMKENVEKVKGLIIEEFQKVSTSLIEKDLKEIK
                     EQLIGNYQISMEDSQDQMVNLLAHEVRGNAKEFYNFEKEISKVKLEDVKNLAKKVVKD
                     YSFFALIPK"
     gene            complement(40018..40809)
                     /locus_tag="PLU55_00265"
     CDS             complement(40018..40809)
                     /locus_tag="PLU55_00265"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86529.1"
                     /translation="MNREELIKKITTKNDFSELPKQDVELAFNKFNRPHYSDEEKVKL
                     TRDLLRKVFSVFTSGKLLNIKDKDCEWILNKHISTRERLKFYPEVYHRIFSDSSLDKV
                     NKRVDLTVIDLGAGVNGFSYKYFPKNINYLGVEGMNQLVNLTNHYFNRENIKGKVIQE
                     SLFNLDKIKGLILKVKGLKILFLFKVIDSLEMLQDNYSKSLIKELTPLVDFVVISFAT
                     RSLVKRERFKVNRKWILKFIEENFEVLEDFEIGNERYIKFRSRFK"
     gene            40843..41751
                     /locus_tag="PLU55_00270"
     CDS             40843..41751
                     /locus_tag="PLU55_00270"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPJ86530.1"
                     /translation="MPINAHPEYIAAEGEYLRAKSVEEKIEKLKKMISYAPSHKGGEN
                     LRAQLKTRLKKLLEEKEKSKKSGKSSRTGIKKEDLQAVIIGKSNSGKSSVLTELTNAK
                     AEVSHTHLSTTTPKIGMMNYQGANIQIIENPAIESEYYDKGITNTTDTLIIIINSIQD
                     LKEIETMIGNIQKNKIVLFNKSDLLNLEEKRKISSTLQSKKYNFVLFSSISKEGIELL
                     KEKLFSSFNKIRVYTKEPGKEKSKNPIILKPGATTREVAEKILKGFSEKVKETKIWGP
                     SSKFPGQIVGLNHKLRDLDVVEFKTK"
     gene            41820..42677
                     /locus_tag="PLU55_00275"
     CDS             41820..42677
                     /locus_tag="PLU55_00275"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPJ86531.1"
                     /translation="MKKKTIGILSCSREIEKEGKLFFEIAKKKGVKVILINLSDKFDI
                     KSFKRKVKQCDIIYCSVWDDFSVELTKTIEEWGIKIIESSKSYYCTEDKWMFYIKCKK
                     HNVPTPETILLSENINNAKSELKQFGRWPVILKRINGTMGEYVEKARGMAEAEQIIKK
                     FWKKGSEKLPIIAQEFIESPSYRVTVIGNKIVQTALKKNNAGWKATGVYAKRFEPFDI
                     DKKLERIIKKTVKFLDIKICGIDVLKKGGKWLLLEVNGQPSLDFFEEEHERIIEEVLN
                     LLIKAAEEK"
     gene            42746..43939
                     /locus_tag="PLU55_00280"
     CDS             42746..43939
                     /locus_tag="PLU55_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HPJ86532.1"
                     /translation="MIIEIIIAIILGIVVGIFTGLFPGIHINLVGTFLVTLSSSFSNH
                     SFMIYLVVFISSLAIAHTFIDFIPSIFLGCPDTDTELAVLPGHEMLKQGKGHEAVMRT
                     AYGGLSGVILLVLLFLPLSFLLSKTYPTIQKVIPYILILVCIIMIFSEKNKLQAFLVM
                     IMTGVLGYLVLDLNLKEPLLPLLSGLFGSSMLLLSIKQKTKIPKQKTNFKKIKLKNMM
                     RPLSGAIIASPFCGFLPGLGGGQAAVIGNQISRTNEKDFLILLGIVNVFVMGLSFVSL
                     YSISRTRTGAAAAVQSLLGQVTFQSLILIIIVCLISGIISFFLTKKLSILFIKYIEKI
                     DYNKLSKWTILFLSVLILIISGILGLFVFGISTLVGLYCIQLNVKKTQMMGCLLIPTI
                     LLYLL"
     gene            complement(43946..47410)
                     /gene="polC"
                     /locus_tag="PLU55_00285"
     CDS             complement(43946..47410)
                     /gene="polC"
                     /locus_tag="PLU55_00285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HPJ86533.1"
                     /translation="MDTKIYFENLKKEVDKVYSVANLARSKGYDPVHEVEIPLAMSMA
                     EKVVGLISTIYPQMKGAGIAERILELEKEYGKLDVTILFKIAEEVAKQKFCKFENLLQ
                     AMDAGIRIAFAYTTLGVVSSPIEGFTGLKIRKTRDGKEYLEASFSGPIRSAGTTASCV
                     ILIVIDYLREIFGFAKYDPTPDEIKRVYAELEHFHDRVTNLQYMPTEEEALFLAEHLP
                     IQIAGDASETIEVPNYKNLERVDTNYLRSGFCLIIGEGLAQKAAKGFRLLGRAKKNGI
                     QSTGFDFLEEYVKIHEKRDRGKADGSPTYIKDLVAGRPIFGHPSKSGGFRFRYGRGRV
                     SGFSATSLNPATMAVTDGFIAIGTQLKIEKPTKGCAVTVCDSIDGPIVKLFNGSVKKL
                     SSIEETKSIYPDIDEIIYLGDILFPFSDLANRNANLIKPGYVEEWWHLELIEKENSLK
                     NINPFSVSFDDAIELSEKYSLPLHPKYIFYWTQISRENLLDLLIWLENARIEKKIILP
                     YNKSEREKFSLGKRALEILGVEHQVTVENVVLTEDISRALLFNLGFELNKVDGNKINF
                     KEIFDFYNLNKDKSVLEIVNSFSKYLIKDKAGEFIGARMGRPEKAKLRKLVGSPNVLF
                     PVGKEGGRLRSLQAACKEGKVWGSFPIYYCDSCQKETIYLTCEECNLITKPMYYFMET
                     KEKLFSKSNENSSREGVRYCNKSIDINHYYEHALKRLGVSKEEAPLLIKGVRGTSSAN
                     HAVEDLSKGILRAKYDLQVNKDGTIRFDATELPLLYFKPKEAYVSVKKLREIGYHKDI
                     FGEELEREDQILELMPHDILLPSSFDSLDERADDVFFKVSRFVDEELEKVYGLEKIYN
                     VQNKEDLIGKLGVCMAPHNCAGVICRFVGFSNTQGLFASPYMHAAVRRDCDGDEAAIM
                     LLGDVLLNFSREFLPSHRGGTQDAPLVLNAKIDAGEVDDQILDFEFLMNYPLDLYRAA
                     EKGVHSSEVKVNTVREILKKGENPFLRAGFTHDTDNFNEGILCSSYKRLLTMKDKVNH
                     QMNLVEKLRSVDTSDTARLIIDRHFIRDMRGNLRKFSMQEFRCVGCNEILRRVPLNGV
                     CPFCRGKLIFTIHEGGIKKYLEPALDLAKKYNLSNYMQQNLELVKRYVESIFGRELEK
                     QENISKWF"
     gene            complement(47412..48380)
                     /locus_tag="PLU55_00290"
     CDS             complement(47412..48380)
                     /locus_tag="PLU55_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPJ86534.1"
                     /translation="MEIKDRVYGVQKIEENVILDLINSNSLKRLKDISQLGMPDTYLS
                     FPGFSRYEHSLGTMILLKKLGATLEEQVAGLLHDISHTPFSHVIDWVVGDPTKEDYQD
                     KIFQEFLEKSDAFDILDQHGLNPYFISNLDNFKLLERDAPKLCVDRLDYSLRQLRIEK
                     GKELTDKILNDLCVYDNQIMFKNFKPAKLFGDSYMGLQKSSWAGDEMRSRYHILSETL
                     KEALNKNLIDFSFFNGTEKPILEILNNSNNEFILLNLSMLKNGFSIIEDNEGIELKKK
                     FRYVDPEIKVNGGFVCLSEICKDYKKTVEYEKERNKEVKKVKIIPV"
     gene            complement(48370..50058)
                     /locus_tag="PLU55_00295"
     CDS             complement(48370..50058)
                     /locus_tag="PLU55_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPJ86535.1"
                     /translation="MDSKQLLKVCLENGLLVDKDVLNLFNGAKDINSVKLIIDKLKNY
                     TRQNIITKNIFYEHKEKVNEFFYELPQKSQKELENFKIKLGLSIEISREKESIVDEKN
                     SLSINSVKSNSSSSSLVYSDSSESSDSSGEVLILSSFPVVNKKLEVQDFVSYFRSRFV
                     EMRGILQSRPELDNPISISKIFGNKQKISIIGIVSKKSFTKNRNIIFDVEDTTGLIKI
                     IINKDKKELYEKAENVALDSVIGFKGSGNEERFFADDIIYPDSALPERKFSLNDEYAA
                     FIGDLHFGSKRFMRDNFIKFINYLNGELDEDKEAKKIKYLFLVGDIVTGVGNYPNQEK
                     DLEILDLEEQFFGLAELLSKIRKDVKIIISPGNHDGVRLMEPQPFLDQKYSWPLYEME
                     NVFITPNPCYVNIGKRENFSGFNVLTYHGFSYPYYANNIPSLIQKKAMNCPEEIMKYL
                     LKNRHLAPTHASTKYFPLEKDGLLIRNIPDIFVSAHTHKCGVTYYNNILVVSVSCWEE
                     MTPYQEKFGNIPDHCKVPIFNLKTRKVKILDFEDNEPVQENKLPSLETTNSNGN"
     gene            complement(50073..51287)
                     /locus_tag="PLU55_00300"
     CDS             complement(50073..51287)
                     /locus_tag="PLU55_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orc1/cdc6 family replication initiation protein"
                     /protein_id="HPJ86536.1"
                     /translation="MKLDIDKIFDSFDKNNIFKDKTILQSNYSPSEIPHREEQIKQIA
                     SILAPVLRGEKTSNLFLYGKTGTGKTLAMRFVQEELYKRVKKDADFKLKIEYLNCKMK
                     KVSDTEYRILAELVKKMGGDVPLTGLPTQAIYNKFIETIDSEKNLVVLILDEIDQTVK
                     KISSDFLYSLVRLNSELSKSQICLVGISNDLRFLEELDPRVRSSLSEEEILFHPYNAL
                     QIQDILNQRAELAFKEGVLQEGVINKCAALAAKEHGDARRALDLLRVAGELAERDNSK
                     KILLKYIDNANEKIEKDKILETISSEPKQFQLVLGSIIYLLEHQKNDLPVFTGEVYDF
                     YQQLCLKNRLEILTQRRVGEIIQELDMLGIINVRVISKGRGGRMREIKMAISKEITNK
                     AKEIIENSLNYV"
BASE COUNT        19762 a         8474 c         6472 g        16810 t
ORIGIN      
        1 aagtgcaact tctgttccat cgatttttcc ttttaatttt ttcaaaaatt catccctcaa
       61 atcttttatc tttgatttta aatcaacttt aataaattca aatttttttt cgtgagtaaa
      121 acctgcacca aattcatcgc ctagaataat aatcttatcc cactctccat gtttcattaa
      181 accagatacc tgtccccatg ttccttttcc agatgaaagt aatgccacta attccatttt
      241 aaagtttctc caccaatctt tctatagata aagcattatt cttagtaaga atttccaatg
      301 cagaagtttt agttatttgg actgggggta aaaaacattt tgggacagac aaatcaccat
      361 tatcatctaa tttaattccc atcttttgga gagagagtaa acttcccaaa atatccaagt
      421 taccctctga tttatgtaag acataagaaa ctttctcagg ggaagattta tatcttaatc
      481 cataatattc atccatatta aaacttaaaa ttctttgttt ttaagtatta ttgcaataca
      541 aaaacaaatt actttaacca gaagacttaa aaacccaaga gaatataaaa aatcaaccgt
      601 ttgaaccata tcggactgga atactttcta tatccagcac gaataatgtg ggaggttaaa
      661 aataagaaaa tggcagaact agaagttgaa ttaaaaaaag gtttatctga attgagaaaa
      721 accaaagaaa ggaaatttga ccaaacagtt gatttaattg taaatctaca aaaattcgat
      781 gttaaaaaaa ataatttaaa tttatttgta agtgttcctc acaaagtaaa agacaaaaaa
      841 atagctggat ttctagaaat taaaaataag gatgttgaaa caataactcc agatgaattc
      901 aaaaaatatt ctgataaagc aaaaatgaaa cacttggttt ctacatatga ttttttcata
      961 gctcaagcaa gtttaatgcc aagtgttgct acaaatttcg gtagagtatt gggtccaaca
     1021 ggaaaaatgc cctctcctca actaggaata atcttaaatg ctgatgaaaa aacaatcaat
     1081 gaactaaaaa acaaaataaa caccagcata aaaataaaag taaaagaacc aagcataaaa
     1141 atcccaatag gaaaacaaag tatgaaagat gaagatatta tagaaaatgt aatgtctgtt
     1201 tacaacttgc ttcttaaaac ccttccaaga aataaagaaa acataaaaaa tattgaaata
     1261 aaattcacaa tgactaaacc atataaaatt tatttaaggt aaaatgaaat ctaaaacaac
     1321 tacaaaaaca aaaacatcaa aaattcctga aaagaaaaag aaagcagttt cagaattatc
     1381 taaactaatc caggaaaaga aaacaatatt agttgtctct ataaaaaata ttccaggatc
     1441 acaatttcag gaaatagtta aaaaattaag aggaaaggca atagtcaaag ttccaaaaaa
     1501 gaatttaata ataaaggctt tagattcttc aaaagaagaa gaaataaaaa aagtaaagga
     1561 aagtataact caggattttg cattattatt ttcagattta gattcttttg aacttgcaat
     1621 agatttagta agaaagaaaa gtccctcaaa ggcaaaggca ggacaaatag ctccagaaga
     1681 tattgaaata cctgcaggac caacagattt agttccagga cctgcaataa gcgaactcgg
     1741 agcattagga attcaaatac aaattgaagg tggaaaaata acaatcaaag ctcctaaagt
     1801 tgtagctaaa aaaggagaaa aaatctctca aggtgctgca gacatgatgg gcaaattaga
     1861 tataaaacca tttagcatag ggtttatacc tgttgcagca tttgacaata ctctaaaaaa
     1921 attctatcca aatattgata ttgacccaga taaaactctt gaagaattta aagaagctta
     1981 tagtaaagca ttaccatttg cagtaaatat aggatatgct actcctgaaa caattacatt
     2041 cttaattgga aaagcaggaa tgcatgaaaa agcaataaat gcactattat ctcaaactcc
     2101 tgaaataaat tctacaggag aaggaaatta aaatggaata catatatgca gcacttttgt
     2161 tgcacaaact tggaaaagaa gttagtgaag ctaacataaa aagtgttgtt gcggctactg
     2221 gcgcacatgc agatgattca aaaatcaaat cacttgtcgc tagtttaaat ggtgtggata
     2281 tagcaaatga attagctaat gctagtttag ttgctgctgc tccttctagc tcagctcctg
     2341 ctgaagcaaa gaaagaagaa gctccaaaag aagagaaaca cgaagctgca gcagagggtc
     2401 ttagtgcact attcggttaa ttttaaattt tttaaaaagt aactaagccc tttacacttt
     2461 attagtttgc acacaaacta aacacatcaa agaactaacg gcaagaataa atgtattttc
     2521 aattctaaaa aatttattat tctctattta aataatctgg aatagaaaac tttttgaatt
     2581 ctttaaataa atttttccca aattttaacc tatatactgc ttctccctcc aagacacaat
     2641 cttctaaagc tgtatgatac tttccttctt taataatttc attgccttta acattaatcc
     2701 tttcatcagg aataccacaa aatttcaata tttttgtcag gttcatacta cttttaccct
     2761 tttcatcttt catatattcc ccatttatct cgcgatattt ttcctgagcg agagtatgta
     2821 attctattgc tctttgtccc ataacctcac acattttatc cataatccca tattttatac
     2881 atttattctg tataaaatta aaatcccatc ctacattttg cccaatattt attttttctt
     2941 tgcagctctt tctccattct aaataattag aaactaactg tttttgaggt tgttttttag
     3001 tattccttaa atacttttca gttttccctg taacttttag tgcaccttct gcaacaatat
     3061 cttcgttatc aattcttcct tcttctaaaa aataattttt tggattatcc aaatcaattg
     3121 acgcaatctg ccaaattcca catcttcctg tgtctattcc agttgtttca atatccaaaa
     3181 taatcatagt cctacaaata ataagttaat ttttatagat ttgtacgata aaaaagctac
     3241 ttcacaaacc caactttctt taaaatttct ggtcccataa tttcccctaa ttctcctttc
     3301 ctacattccc tttcacaaaa tttctttgct acaggaacct ttccacaatt ataaaataaa
     3361 acaggaacag gatcagcaga atgtcctttt aatttacaag gagtggaatg atctcctgta
     3421 acaaggactt ttattttatt ttgaggagca acacctctta aatatttaaa taaagttata
     3481 tcaatatact caatcattaa tttcttttca atcggcttat tatcatgccc tggcaaatct
     3541 gtttctttaa tatgtatata tgcataatct gccctcttga gatttttttt aattacattt
     3601 acagaatgtt tacatgcttt tctaagccct ttccacaaat tttcatatgc atcaattcca
     3661 gttaattcag gataatcgaa agaataattt ttcatcccgc acgcatttga aaaaccgatt
     3721 tctaaaggca tataacttgc agaaacccaa ttcttatatt gtttaagttt aggaggctca
     3781 attcctgctc ccctaatcaa taaaaaattc gcaggtaata atcctcttct aactctttct
     3841 acattaacag ggtgcgtatt taaaatagaa aaagcctttt ctaaaaattc attaacaata
     3901 tttgcagtat actgggaatt ttcttcttca tctaatgctt tacatttaac aatcctattg
     3961 gatattaaat ccataccatc catatatgtt gaatcatttc cagaaacatt atcagaaaaa
     4021 ccccctctaa aaaccaaaga agccctgtgt tgaatagttg gcttaaaaac aaatttgcaa
     4081 ggcattttta ttttattcaa tgattttgca agttgattag cctcagaatt tgttaaagtt
     4141 cttcctgctc tcctatctaa gatattccct ttttctaaag aatcaattgt accaaaatta
     4201 actctaaatg ctaaatctcc cctagttaat tttaaattac ttcctattgc ctccagttgt
     4261 cctcttgtgc ttgaaatgag ttcattccca aaaagagaaa ctattgcctc gtctgattct
     4321 gggacaaaac ctggtttaac agtatgaaca tatcctaatt gtcctcttga agccaaaaaa
     4381 tcaagatttg gtgtttgcgc ggcctctaaa ggagtcatat cctcaagcat tttatgaggt
     4441 aaatcaccca atccatccaa tataataaat actcctttca tcataatata atgtatatcc
     4501 ttttatttat aaaaatttag attaatcatt ctatctctaa aacaaactaa accaaaacct
     4561 taataactaa ttatttgctt taaaaaacat gtttggaaaa ttaaaagaaa aactaaaaaa
     4621 ctggactcag ggactaataa aaaaaacagt tgaagaagaa tttgaagaag aaattaaaaa
     4681 aacaaaggaa aaagaaaaga aacctaaaaa acaatctaaa gaaaaatttc aagaagaaaa
     4741 aaaagaaaag aagaaattaa aaaaacaaga aaaagaagct gaagaaataa ttcaagaaat
     4801 tgagcaaaag gaaaaggcaa aagaaaaaat aaagccaata gaagaacatg aatatgtcaa
     4861 accttctcca aaaacctttg acaaagaaga aagagcaata agtgaaaaaa taatacaaga
     4921 tatagaagca gaagaacaag aaaagaaagg attctttaaa aaagtatttt caggcataag
     4981 caaggtaaaa atatctgagg aagaatttga atcttacaaa gaagacctag aaatgattct
     5041 tttagaaaat aatgttgctt tagaagtttc agataaaata atacaacaat taaaagaaag
     5101 aattgtgggc aaagaattcc ttaaaaaaga aatagaagga caaataaaag aatctcttaa
     5161 agatataatt tatgaaatac tcctagaacc ttttgattta attgaaaaaa taaaggataa
     5221 aaagctttca gaaaagaaac cttatgtaat tttattctgt ggcataaatg gaacaggaaa
     5281 gacaactagc attgctaaaa ttggaaacct gctaaaagaa aaaaagatat cttgtgtttt
     5341 tgctgcaggg gacactttca gagcagcttc aatagaacaa ttgaaaaagc atggtgccaa
     5401 actaaatatc ggagttatag ctcatgaata tggttcagac cctgcttctg taggatttga
     5461 cgcaataaaa tatgctgaaa aaaaccacat agatgttgtt ttaattgata ctgctggaag
     5521 aatgcacact gaaaaaaacc taatggcaca aatagaaaaa atagcaaagg tctgtaaccc
     5581 agatacaaga atatttgtag gtgaaagtat aacaggaaat gatataattg aacaaataaa
     5641 aacttttaat caatcagtta aaatagatgg aataattcta agtaaagcag atattgatga
     5701 aaaagggggt acggccctct cagtaggata cacaacagaa aaaccaatat tatatcttgg
     5761 aacaggtcaa gaatacaaag acctacaaaa atttgataaa aataaatttg ttgatagctt
     5821 aggattataa acgccgtaac caggaatcga acctggaaat cctttcggat cacgatttcc
     5881 aatcgtgtgg agtaccattg tcccattacg gcataaaaat cccatttaat aacaaaccca
     5941 aactttatta acttataatt tgctattttt tctatgttag aaaagctcgg agaagcatta
     6001 agaaaggcaa gagataaact agctaatgct ttatttttag ataaaaacct agttgaaagc
     6061 gtagtaaaag aattacagag agcacttatt gaagcagatg taaatattat tcttgtaaaa
     6121 gacatctcag ataaaataaa gaaagctgct tatgatgaaa ggataaaaga aatagagaaa
     6181 aaagaacaca taattaaatt actacatgat gaattagtaa agattcttgg agaatataaa
     6241 actcttgaac tcccatcagg aaaacaatcc agaataatgt tcttaggttt atacggtgca
     6301 ggtaaaacaa ctacaattgc aaaactagga aattggtatt ctaaaagagg aaagaaagtt
     6361 ataatggttg gattagatgt tcatagacct gctgcaaaag aacaattaga acaattaggt
     6421 aaaaaaaata atattccctg ctttgttgat ttcgatgaaa cagatgctgt aaaaacttgg
     6481 aaaaaatttg aaaaaaaaca tgacttaaaa aaatatgatt taattttagt tgatacagct
     6541 ggacgccata ctctggacaa agaactaatt aaagaaataa aagaattaaa caaagaaata
     6601 aagacaacag aaacaatatt agttatgcct gcagatatag gacaagctgc aaaaaaacag
     6661 gcaagtgaat ttcaagaagc attgaagatt tctggagtta taattacaag aatggattca
     6721 acagccaaag gaggaggagc attaactgca tgtgcagaaa caaaagcagg agtatattac
     6781 ataacaacag gtgaaaaaat aaatgacata gaagaattca atccagaaag tttcctttca
     6841 agacttttag gtatgggaga tttacaggca ctaatagaaa aaatacgctc tgttacagat
     6901 gaagaccaac aagaaaaaat gcaaaagaga cttgaagaag gaaaactctc tcttgaagat
     6961 gtaattgaac aagtaaaatc aatgaaccaa ataggaggat ttgataaaat caaaggcatg
     7021 attccaggat taggaaaagc aaaaatacct gaaggcatgc tagaatctca acaagaaaaa
     7081 attgcaaaat gggaacatat ccttaaatct atgacaaaag aagaacgtga aaatccagaa
     7141 ttatttgata aacaaacttc aagaatagga agagtagcaa aaggggcagg agtaaataat
     7201 agcgatattc gtgcccttct caaacaatac aagatgcttc aagatttagt taaaggagga
     7261 atgactgata tggacatgtc taaaggtttc tcgcaaaaac aaatgcagag gatgatgaaa
     7321 aaattcggta aaaagaagat gatgagattt aaataaaatt caaaaaatta aaataaaaac
     7381 tattaaatac taaccctttc aaacaattca atggaaaata aaacaaaata cacaataatc
     7441 ttaacaatat tcctaatttc attaatattt tctggaatat taacatttgt atctcttgaa
     7501 gaagcttgcg gaggaattca aacaacttgc tatgctgttc aaacttctca atatgaaagc
     7561 acatttggaa ttaaaaattc caacataggt ttagttgctt tctctataat ggcgatactg
     7621 gtatttttac atataaaaca acctacaaaa tataaaaagc aaatgatagt agcaggaata
     7681 attggaggaa ccatctttgc attatacttt ttgtacattc aattttttgt aattgatgcg
     7741 ctatgcaaat attgtatggt aatagatatt ggaatgttac ttaatttagg aataataatt
     7801 ttttggaaag acaaataaaa gatattagtt taaataaaac ttattttaat ctaccttcta
     7861 aagaaccttc ttttcttttt ttaacattaa gaaatctagt tgaaaaacca attataactc
     7921 tcttgccttc tttttcaaca acagtcattt caataggata tgaaaaatga aaatcatctc
     7981 ctggttcaaa tttaattgcc tcattcaaac cagaagaata aaaagttgta tctccactct
     8041 caattttatc aattttcaat gaataaggca agcccatcaa aaaaaagtgt aaactatctt
     8101 tatctttaaa accatcctct ctaaaatctt taagaatctc tggaaaagtt ttatatttct
     8161 catcaagttt ttgattaatt agtttctctt taacatttct gagtctcccc atctctacag
     8221 aataatctaa aagaatatat ccagaacctt tagcataatc aaaaaattct ttcttaaaca
     8281 taaaattctt agtttcatta taagtagagt cattaatttg attaaatttc attgaataaa
     8341 tccccaacaa aaaagaaaaa tctatttctt tttcacttaa attaataaaa ctagttgtag
     8401 ctggaaatcc tccacccttt gttcgcacct cataattcag tttatcctga gaaaaagaag
     8461 gcaatatagt tgataaagta agtgctcctg taaggaaaat gtccttaaat ctcataaaaa
     8521 aaggattgaa aataagtata aaagcattta tatagaaatc ttagtaggac aaaacttaaa
     8581 atatatagaa gattaaattt taaatgaaat taattcaaca aggagcagaa gctaagatat
     8641 ttttagatga aaagaaaaac caaatagtta aagaaagaat atctaaatca tacagaatac
     8701 ctgaaataga taaaaaaata ataaaacaaa gaacaaaggc agaaactaag cttattgaga
     8761 aagcaaacaa aacaatacat aatttttcac ctgaaatctc aagggaagaa aataaaataa
     8821 gaatgccgta tatagacgga gaaaaacttt ctgaagcgct aaacagtttt cctcttgaaa
     8881 aacaaaagag aatattaaac aaaataggaa aatcaatctc taaattacat aaatcagata
     8941 ttatccatgg agatttaact acaagtaata tgattttaaa agacaataaa atttttctaa
     9001 tagattttgg gcttggatat atttctaaaa aaatagaaga taaagcagta gacctccatt
     9061 tactaaaaca ggctctagaa gcaaagcatt ttcaacactg gaaagaactt tttgaagaat
     9121 tcaaaaaagc atattcaaag gattatcctg aatccaaaca aatatttgaa agaattattt
     9181 ctgttgaacg aagaggacgt tataggcatt aaaacaagat ttaaataata aaaatatcta
     9241 agatagatat gataaagatt ttttcgtttt caaatggaaa gttaaaagaa gtagatccaa
     9301 caacattaaa aaaagaaaga aggacttctt attagataga tttgttctgt tcaaatgttg
     9361 aagaaataaa atcaataaga gaaatatttg atatacaccc tgcagcagaa gaggacatac
     9421 tttcaaatca aacaagaaca aaatatgaag aatttgaaga aaatactgca gtaataatgc
     9481 agggcataaa agagattgaa gaattcaaca taaaaatata caatttatct tttatctttg
     9541 gtgaaaatta tcttataacc tctcatttcg aaaataacga agcaatagat tctctaataa
     9601 gcaatcctaa aaaattagag aatttaatga aaaaaggagc aggtcatata ttccattacc
     9661 ttttagataa agaaatagat aaatgtatgc aaataaaatc tatattgtta gaagaattta
     9721 aacaaacaga aaaagaattt ataaaaaatc cagaaaaaga agttttagaa gatttattcc
     9781 aaaaagaatt gactctgtta gaaacaagac aagtaatgga atctttaact gacctatgtc
     9841 tcagcttaac aagaccaaca gataattact tagataatga attactccct tattttagag
     9901 atatttacga ccattctttt agaacaacag aatcactaaa aagcatgtta ggaagaataa
     9961 atggtatgag aaatgcttat caaacaataa cttccaacag gttaaatgaa actatgaaga
    10021 ttttaactat gattatggct ataatgatgc caataacaat tgtaacagga ttttatggaa
    10081 tgaatgttag actcccatta gaggataatt attacagctg gatgtggata ctagcaataa
    10141 tgatttttgt atctttgata atgtatctta ttttcagaag aattaaaata aattaataaa
    10201 atattaaaaa ttaaaatagc cccaccagga ttcgaacctg ggtcacggcc tctcttcgtt
    10261 tatctaaact ccaaagggcc gtatacttga ccgctgtact atggggcttt ttacacaata
    10321 aaagagggtt taataatttc ttaaacctta ctatttgaga aaaataaaga tataaggatt
    10381 tagtatcaac tcattctttc aaataaatct aaaacctcct ttcattattt aacaaatata
    10441 ttaaaaatat gataaaaaag agacaaaatc ataagcttta aaaaacccct ttacgaggta
    10501 tttagatgtc aactgaaaaa gatgaaaagc caaagaaaga acaaggaaaa atgagcttag
    10561 aagagtatga aaaggaagta attaaactag cagataaagg actaacttct gaaaaaatag
    10621 gagaagaact aaaaagaaga ggaatccacc caaaagaata caataaaaaa atctctaaaa
    10681 tactaaaaga aaaatatatt aatccagatt taaagaatgt agaagaaaaa ttagaaaaaa
    10741 tagaaaaaca tcttaaaaac aacaaacaag acaaaagagc aatgagagaa agaagcagag
    10801 tattctctca agttagaaag ataaagaagt atcaaaagaa attataaatt ttcttatatt
    10861 aaaatgaaaa aagaggatga aatattcgaa aaggaactta aatcatttgc tgaggaattt
    10921 ttaaaaaaaa cagaaaataa agagattctc ataatgagtc actttgacac agatggaata
    10981 acctctgcaa gcataataat aagcactcta aaaaaattag atagaaagtt ctctataaga
    11041 atattaaaaa atttagaaga aaaaacaatc aaaggattac caaaagataa aataatattt
    11101 ttcttagatt tagcatctgg aagtttagaa catataaaaa acaacaactt aaaggatgta
    11161 tttattattg accaccacga aatttcccaa aaaattccag aaaatgtaag aataataaat
    11221 ccgcatttaa caaacaaaca aaaaataagt acttctggac tagcatattt attcgcaaaa
    11281 caaataattc cagaagcaaa agaattatca aaattagcta tcttaggaat gataggagat
    11341 tgcttggaaa aagaaataga ttctttaaat catgggatac tggaagaaag cggagttcaa
    11401 agaaagagag gattacttat ttatccatca actagaccat taaacagagt attagaatac
    11461 tcatcaaatc cttatatccc tgaagtcact ggaaacctta aaggagtcct ggaaatatta
    11521 agagaatctg gaattatgcc tgaaagaggg aaatataaaa gtataatgga actagatgaa
    11581 aaagaaatga agaatttaac tacttctata cttcttagaa accctaaaat aaaaagcaag
    11641 gacttagtag gagatatttt cttgataaaa atgtttaata aaatagaaga tgcaagagat
    11701 ttaagtgcaa taattaatgc atgttcaaga tcaggtagac ctgaattagc aatacaatac
    11761 tgtcttgaaa taccaaaatc aaaaaaagaa gcagaattag cccacatcaa atataaacag
    11821 aatataatca ctggattaaa ctttgttcaa gaaacaaaga aaatacaagg aaaaggattt
    11881 gtcataataa atgctcaaga caatgtaaaa gatacaatga ttggaacaat tgcaagcata
    11941 atttcaaatt cttcaatata tgaagaagga acaataatca caacaatggc atattatgaa
    12001 gataaaataa aggtttcttc aagaattgtt ggaagaaaag gaagaaatct aagagaatta
    12061 ttagcaagtg taacaaattt aactggagga gaaagtggag gacatgaaca tgctgcaggc
    12121 tgtttaataa gcagaaaaaa agaaagagag ttcatcgata ttcttaaaaa aaatctcgaa
    12181 atagagctaa taaaaattta agaatctata aacacaacct ttaaaataga gtattctgtt
    12241 taatttttat ggtatttgaa gaaggagaaa tggtattgtg tactgtagat agaattgcag
    12301 gcacagtagt atttgtaaaa atagaaggag aacaaagaga agggagcata attttaagtg
    12361 aaattgcacc aggaagaata agaaatttaa gagaatatgt tattccaaaa aagaaaattg
    12421 tatgcaaggt tctaaaagat acaggaggaa caataagtct ttctctaaga agagtaactc
    12481 cgaaagaaaa aaaagaagtg atggaccagc ataatcagga aaacagttat ataaatatac
    12541 tcaaaagcgt cttaaaagaa aaatcagaag aagttataga agagatttcc aaaagaggaa
    12601 gaatatacga gttttttgaa gaagctagag aaaaccccaa gatattagaa aaactagtag
    12661 gaaaagaaaa ttcagaaagg gttttagata taattttaga gcaaaagaaa aaaaggataa
    12721 taataaaaag gatagtgtat ttaaaaacat tagattcaga aggcatatct cacttaaaaa
    12781 aagtgttaga tacacctaaa gaaatagaag taaagtatat ttcagcagga aaatactctc
    12841 tcaaaattga gacagaagat ggaagaaaag gagagcaaaa aatgaaaaac acactagaag
    12901 aaatagaaaa aaaggcaaaa gaaaaaggaa tgatatttga aataaaggaa aaataaaatg
    12961 gcagataaat ttaatgtaaa aatcatagga attttattca atcccccagc aagaaaaata
    13021 cttcttggca ggaatattgg agaaagaaat ttaagttttc tcgaaggaga tttaaaatat
    13081 gaagaagaat tagatgttgg attaaaaaga gttgcaaaag aaaaaacagg atatataatc
    13141 cataatctag gtgcagttta tgctgaaaac agattaaaag acaaacaagc attaagatta
    13201 tattttttgt gtgaagcaac agaaggaaaa gaaactcctg ggaagaatgt tgcagagtta
    13261 aaatggatat ctccaaaaga agttgaaaaa caactaaaaa agaagttgcc tacaaggcta
    13321 aaaggatata tactcggatt aggatgaaac tcaaattcta tattaattca aataaagaaa
    13381 aaatatacac tttagatgaa gaaatacaag gacaaaaaac tcaagatgct cattacaaat
    13441 ttgtaaaaat aaaagatgct tctcattcaa atagattaaa aatacaaaaa aacactaaaa
    13501 catattctaa aaggtaacaa tttttataag caataatttc gataatcatc tgatgaaaaa
    13561 gagagaggtg tatgaaaaga aatacagagg taaacaagta gaagattatt ataagttgat
    13621 ttcaaacata agagaaattt cttttgaaga accaactgga ataaatagca attctatatc
    13681 aaatacatgg ggagaaaaag gatatatcag ttttgatgga gattttcttg taagagtatc
    13741 taaaaatcaa ttaaatcaat ctagcaaatt aataaacaaa gcaaaaataa cagtagaaaa
    13801 aattggacca gatataagca aagagtcaaa aatagaaaag atgttgttag aaagaggatt
    13861 taaaaaaata atagaaagaa aataaaacta ttttgctttt tttataacta tatgcttggg
    13921 taaatccata tattccttta aatttttcat ttcagaatca tttaatggaa aaatagaagt
    13981 ttcaggataa aatgattttt taagtttctt tgaaaaacca ttatacaaaa ccatttctgt
    14041 atctaattta gaaaaacatg ctcccttaat ataccctgca aaaactttat attctaaatc
    14101 ttttatgaga acttcattct caattccttt aaatggattc tttttcaagt gaaaaattgt
    14161 gtaaactggt aaagatttcc cttcaagaag atttttatca acaactgcaa acgaaagttc
    14221 ttcttcataa tttcttgaaa ttttgttttt cataaaaaag aatactatat accactttta
    14281 aagattgtta tactttaaaa caatgtagcc tgcttttctc cgtctactaa ttctccatat
    14341 ttaccatcat accctggttt aacctttatt tttccttccc gattttccaa aataagttta
    14401 actaactttt cattatcttt taactcttta atcaaatctt ctttatatac attaagcaaa
    14461 atattaaatt cattttcaaa cttttcaata agcatattat atacttccca agttttttta
    14521 ctttcaatac ttgaaccaat actagatgca attaattcat gtaaaggtaa caaggtataa
    14581 tatttttttc tctttctatt ctcttcttta atctggtctt taagttctaa tgctctattt
    14641 gctacaccaa ttgttagagg atgtttacaa acagggcaaa taccatttaa ttttttagtt
    14701 tcttctggag aactagaaaa accacaatct ctatgtccat cataatgata aataccataa
    14761 ccaggatcag tttcaattgt acctaaaata gaattatttc ttatcccatc aattatatcc
    14821 ttaaaactat ctgctttctc aaaaattgtt gcttctctcc cgattctcca aggccagaaa
    14881 ctatggctgt cagaaaaact cactatagct ttattgttta attcagacaa atgccaattc
    14941 atctctggat ttgagctcat tcctgtttca attgcgtgta tattttcaaa ttcttctcca
    15001 aaagcttctt ttaaactatc aaaacctgat ttacttccaa aaactccaaa ataaggagtc
    15061 caaacatgtg ctggaataac ttcaatatct ttagatattt ctctcatgtc tttaacaaat
    15121 tgttcacagg aaatcttaaa tataggacgt ccatcatagt cccttcttcc ttttttatca
    15181 aggtaagcat ttattttgtc tgcaatttca aaattaggaa ccaataaaac taaatgaact
    15241 cttctccctc ttccctgagt atacattaaa cttacttctc ctgttagtag aaatttaaat
    15301 ccgctttttg aatacaaaat tccgtctctc tcagttaatc tttcttttaa ttcattcagc
    15361 caaattggat gagtaaaatc tcctgtcccc aataatccaa ttccttttac ccttgcccat
    15421 ttttctaaat tttccaaagt aatatccttg ctacatgccc ttgaaaacct tgaatgaata
    15481 tgtaaatctg caaaaacacc tgttttttcc attttcaaaa caataaataa aactttttaa
    15541 actatattgt aaaaatatat tcattatgac agaagaaaac aaaaaagaaa gagcattaat
    15601 cctaattaaa ccagatgcaa tagcgagaaa gctctctgga atagttattt atgatttaga
    15661 tcatttaaat ctaaagatga ttggattaaa attagttagt gttcaaaaac atcttgcaga
    15721 aaaacattat gcagtgcaca aagaaaaacc attctataat gatctcctaa aatatcttat
    15781 gggagaatat cataatgttc atgcaattat tgcaatcgca tacgagggag aaaatgcaat
    15841 acaaaaatta agggaatatg ctggaaaaac taaccctgac gatgcagaac catcaacatt
    15901 aagaggaaga tatggaaagg ttaattcaaa aacaaataac tttgataatg taatgcatat
    15961 ttcagattct cctgaaaatg gagaaaaaga aatttccatc tggttttcaa aagaagaatt
    16021 agttgattaa aaaattaatt cttatctaag aatttaatta aatagttact ctttatcact
    16081 tataccgcca gcagtaacat aatacactgt ctcattatct ggaagatttg gactgtcaat
    16141 taactttgca actctagaac cttgttttcc tctccttaaa tataatctat aagttgaagc
    16201 atgtccaaca atatttcctc caatagcagt tgttgggtcc ccaaacattt gtgcagggtt
    16261 tgccataacc tgatttgtaa cataaacagc caaattatga gtttcagcta gtttcattaa
    16321 atcatgcata tatctgttaa gtttctgttg tctatctgct aattgtcctc ttcctgaaaa
    16381 ttctgctctg aaatgagcag ttaaagaatc aacaatcaaa agttttattg gttcacctgc
    16441 tttaaccatt tcagtaactt tctctaataa caacatctga tgatctgaat taaatgctct
    16501 tgcaacaaaa atattcttca gaactttttc tggatttgca ccaagacctt cagaaatttg
    16561 tcttattcta acaggtctga aagtcccctc tgtatctatg aaaacacact taccattagc
    16621 acctccttgc tcaactggca actgaactct gactgccaaa gtcagaccca gctgagtttt
    16681 tccagaacca taagcaccaa aagcttctgt aatggctctg ctttcaatcc ctcttcctcc
    16741 caaaagttca tcaaagtttt tacttcctgt agttatatat gaaacattag atcttctctg
    16801 agcatattca actccatcct gaaatcctaa atcaagcatt tcacgtgcag cttgtatagc
    16861 ttttctagaa acagcaggac ttacaccaga aatatcagaa attgtagcag gacttgaaac
    16921 tgccaaagac attaaatcaa atattcctgc tccctcaagt ttagcagcaa tagctggtcc
    16981 tatacctggc aaatccatta aagtcggttc tttagtttcc ttctttttag atttcttttc
    17041 ttctttctcc tctttttctt cttcaatatc ttctttttta gcagccatat ttactcaaac
    17101 cccaaccagt ttataaagat tatttatata ataaatatat attaaacgca tattagtatt
    17161 cttataacct aaactattca ataaaaaatt acttcttctc aagttgagaa attaattcat
    17221 ctaaattaac ttctttaact ccgtcaacta taaattcctc attattgaaa aaattatttt
    17281 ttcttacatt tccagaaaat atcatttctc tgcctaacaa attttcaatt tgataagata
    17341 atctctcagg attttctaaa tcatttaaac ctaaaacaga aagattgtca ttaaaaataa
    17401 ctgccctaat tgtttctgta ccatcatcaa taactatgtt aataagtgct ttcctatctg
    17461 tagctactcc tttctccctt tcactctcag tcaatttctt cccagtttct ttactaactt
    17521 caaaaaatct aggttgaaat gtttgaacaa taaatgctct tacagaaata gaatctccta
    17581 atttaaactc ggaaatcttt ttctccctaa ttattctgtc tgtcattact tcacccatct
    17641 ctttgctact tcttttaaat tcagaaaagc ttccaagatg catttcattc tctctcatag
    17701 aaccattaga aatatccaca acatctcctt cagttatttc acctttctca attaatgcta
    17761 tatgattcgt gtcccaaata acaactttta tatttgaagt ttcatctgct aaaacaagat
    17821 taagcacctt tccatcttct cctttttgtg ttttaaaaga cctaacagga aaaagacgaa
    17881 ttatctttcc tgaaatatta acttttctca taccaggaag cagttcttca atcttgagct
    17941 tttcgttttc aaaatttatt cccaattctg cagcaactat ttgagcagca ccttcttggc
    18001 taataagtcc agagagttta gccctctttg cttctatttt ccgattaact tcatctacac
    18061 taagtccaga atgttttgct atcttttcaa ctattcttcc ataatttcct aaaaccataa
    18121 ccaaaaaaaa ttaatgtatt ttataagtat tttgatactt taattaaaaa caacagcact
    18181 caaaatattt ataaacctct ttctttaaac taaactatgg taggaataaa tggagtaaaa
    18241 tataagctaa tgccagaatc tacagaagta gatttaaaag aaatggaaaa tgctgcaaaa
    18301 aaaatagttg aagaatttgg aggtacaaat aaagaatatt ccattgaaaa tatagcattt
    18361 ggtttaaggg cattaatagt tttctttttt taccatgatg ataaaaatat agatgttgtg
    18421 gaagaaagac tctcaaaaat acccaatgtt gcttcagttc aactaataga tatgagaaaa
    18481 atagcttaaa gtgcataaac ttattaatgc agaatttctt gataatatat gcccctttca
    18541 aaagaagaaa tagagaattt taagaagaaa ataatattaa atatagaaac aaattttcca
    18601 gaagaaagaa aggctgcagc tgtagaaaaa ataaaatcaa tgaatgataa cgaattcata
    18661 gagttcttaa aaaacaatga tttaatagaa tccgaaaaag aaggaaaaga aaatagaaac
    18721 aataattcaa ctccttttag gatgataatt gaagagaaaa taccttccta caaaatacat
    18781 gaaaacaaag aagcagttgc ggtacttgaa ataaacccaa tatctgaagg acacacaatt
    18841 gtaataccaa aaaaaccaat agcagattct aaaaaaataa caaaagaaat aatttcattg
    18901 gcaaataaag ttgcaaagac aataaaaaac aaattaaaac caaaagaagt atcaatttct
    18961 ccttcgtctg ttttaggaga aataataata aatattttac caatatacac aaatgaaaca
    19021 ataaattcta ataaaaaaca aatatcacaa gaaaaattag aagaattaaa aaagatttta
    19081 gaaacaaaac caaaattaaa aaaagaaaaa aaacctactg taaaaaaaat aaatacatct
    19141 aaaataataa tacccagaag gattccttaa ataaaaaata taattaaggc caaatagcta
    19201 attaaaaaaa caggactaaa gggtatgcct tgtcttattg aaacaaactt catatttttt
    19261 cttaaaaatt gtatttcaga taagctcaat ccctcccaat gtgcttcaat aactctcttt
    19321 cctattttaa catctttata caaccaatct ccttcagtaa gttcttttgt attgactttt
    19381 ttaaccatac aagattcatc tatagattta gcaaaaaagt aaatataagg agagatgaaa
    19441 aaaagaatac ccaaatataa aaataaactc tcaacaaaag aaagaactaa aaaaactata
    19501 cttacaatta tagatatata gaaaatcttt ttatgttttt taaagcgcga ggcaaaatct
    19561 ttggaaaatt tctttttatc ttttaatccc aaagctactc caaatataat cccatataat
    19621 gctccagaaa ctaagaaaag taaaaggaac ttaagcatga tattcaaatt ttctggaaaa
    19681 ttattagata agggtataat tgctccaaga gcaatcatta atttggcatc tcctcctgca
    19741 aaaactcttc catagtaaag catattccca attacaaaaa agatagcaaa accaattaaa
    19801 ccttgataaa aaaaaccaaa tccctcttca gagaataaag agaaaaaaaa tctaaatgca
    19861 atcgcaaaaa taataaggga aaaattaagc caatttgcta tctctctttt tcttaaatcc
    19921 tgaataactg caaaagctat ccagattaaa cataaaacta ttaaaaacac ctcttttaaa
    19981 gccattaata aaaaatagca agtttaattt ataaacctat caaatcaact aaatttatac
    20041 agaaaattta cttaaggcta acaaccctat attttccacc ttttctataa ggatgttttt
    20101 tcttttcttt ccttttatct ttgcttttgt ttcttctttc agaattagcg tgattagaca
    20161 ttttaaatta aaaaaattta atcagtacat agaccgggac aagttggaat tccataattt
    20221 cttgaagtgt atacagggtt agtagctaaa tcattacaag tagcttcaaa tttaggattc
    20281 ttcccgtctt taacttcttt cattacagaa caataattat acttagaact tgttgtacaa
    20341 gcagaattac atgaagtagc aattgtgtct aaattatttt ttcctcccat caattcactc
    20401 aagggtttcc accctgacct aaaacctaaa actaaaacaa ctaaaataat tattcctaaa
    20461 accaacaaaa ttattgtgct tgtgctcaaa ccctgagctt ttttgtttat catcttcttt
    20521 tttcgaattc gagtaatcta attactcaat ctctatttaa aaatctttct ttcatgggcg
    20581 cgggttatat tctacagaaa agattacttt ttccttaaac caattaaatc cccattctgg
    20641 agaaaatttt gtaacttttt tatataaaaa taaatgactg ggattatctg attcaatttg
    20701 aatatttgct ttttccatta tctcaactcc aataagagat atgtttatcc tataagaaga
    20761 agcgataaaa gtaaccattg ctaaaataca cactccaaaa acccctataa ttaaaacagt
    20821 tacaggtata tctccctttt tatctccctt aaaattttta gaatagctat ttttttgaag
    20881 tcccatatta tcctaaatcc tccttttgag aaatccatgt aactatttta tttctattcg
    20941 cattatttaa agaatatgcc atttgtgttt tagaatcaat ccaactcaca tcttcttctg
    21001 aattagcttt aaaattaacc ttaagtgatt tagatttagc caatatagaa gaagcaaaaa
    21061 taaaaattaa aagtatagct atcagaacaa ttgtagcaac tatccatgta actgtttccc
    21121 ctacctgtgc ttttttactg tttaacattt ttctttgatt tacctatagc tgataatatt
    21181 ttaattaaat actggttgtc tttactatca acagcataaa acctgctttc tgtacacttt
    21241 gcaagtcgtt gatattcttt agaattatct ttttttatat aacattcgct ctcccaatta
    21301 agattgccat cataaacact aaaaacaggg ttattcaaat ctctaatcag ataaaaatta
    21361 actctataaa aatattgtat ctcattttcg tctccataat cttcttcaac atcaaaattt
    21421 atattacacc tctcagtaaa tcccttaaga aggttctcat taaatctttc acctacaaaa
    21481 aaataagaag gaataactcc ccttttagaa atacaatctg caagcttttg tgcaagtaat
    21541 tcagattcta tttcccttac atcatgagga gcaccataaa aaatatatac cattgcaaaa
    21601 acacctccgg caactaaagt taataccaaa aaccaataaa tagaaagcat tttatctgtt
    21661 cccctcttat ccatttaaat ttcctccttt tggtttaact gtaaaaagat acgttccaga
    21721 atattttcct ttttcatctt taagggctct agaagaaaca tctacattat taaaaaaagc
    21781 ataactatat ctcccatcag gagttagttg aacataaaca acattatttt ctattataac
    21841 agaatttcca aaatcaaatc ctttttcttc agcaattttt cttcctttat ccatatctaa
    21901 tctaataacc atccccggtt tagcagaatt tattaccatt gcaatttgtt ttgaatatgc
    21961 ttcttctaaa actgttgcac cacttccttg tttcactagg aataaaacca atatagaaag
    22021 aaaaagcaaa tttaaaatta aaaaaactac attctctaca agaatagctc ctcttttttt
    22081 cattataaaa ttatgaaagt ataattaata aaattatcta agtagaagtg agtatatcat
    22141 aacaattcaa cttctttaaa gaatttgaat ttgcttcttg aattgtagga gccatgaaag
    22201 tatttttaac tccatttcct tcaaccaaaa tagcactaat ctctggagtt actccttctc
    22261 caacaactcc tgctccaaca atcaatgctc ctgaaattaa gccaacccaa gtatatcctg
    22321 taacaagtcc aacagcggct acacctagtc caaaaacaac tttccacccc gtgcccttta
    22381 cttcactggt aatacctgtt aaaacaaaat gttgtttgct taaatctatt gttccaaaag
    22441 taccaacaac catttctctc ccattatcta ccttaagagc agtacttttt aatttattga
    22501 tatcatttgt cccaaaaaga tattctgaat aagttacttc actatctggc atttttgttt
    22561 tagataaata atcataaagc ttgtctttac ttattgtttc tccaacccca tttatttctt
    22621 taatactatc atcaaaacct acttgagaac aaattgaaca ataattatcc ctcaagaaaa
    22681 aatctttacc tatataatct aattttcctt ctccaaacat ccaccaacaa tcggccatct
    22741 cttctgcaag aactttatat atttcttctt cggttttcac ttttttaaca tctggactac
    22801 tcatcctctc acaactccca tctttactta aacaaatata agttctagaa cattttaagg
    22861 gaatagcttc cttagatata cttgcagacc tcataacaac agaattatgg caaatttctt
    22921 tatctgactc ttttcctaaa tccaatctaa aaagtaaaaa taaaatgact ataaaactaa
    22981 ctaaaagaat tacaattaaa acaatctgct gtgttgttat ctctcctttt atattcctca
    23041 ttttaaataa ctcctgtttt ctttagcata acaaacaaac ctgctaaaac aatcccaaaa
    23101 aagattatcc atattaaata ttttatgact tcatcaaagc tcatcttgtt tcctctataa
    23161 aaattctccc gccgctattt ttaatttcaa taactaaatt tcttctagtc tcctttattt
    23221 taatatctat atctgttgaa gcaagattag gtacaattaa acaagcccct ttttcatcgc
    23281 atttctttgc ttgagaattt aacttcttga ttaaggaagc ttcacatata cacaaacact
    23341 tttggtctaa gcaactattt ggcttttctt ctctaacaaa actataaata tgccatcttt
    23401 ctgggtctga aatatcttta acctcgctct ctccatcatc caaagaagaa ataattcttt
    23461 ctattcctgg aacactctct ccttccttac caagaagcac ttcttttgct aaaataatct
    23521 ttttatcagc attatgggaa ttatatactg acataagtat atatactaaa aagagtatac
    23581 aaattactgc gattatgact tttaaagttt cttctgctaa aagaaaccct cttttatttt
    23641 tttctaaatt aaccatatta aattattaaa gaagctaaaa atttactagt agaatttgct
    23701 ccaaaattct tgaaaaaatc aaagacatta ttactaaaaa aataataaac tgcgtatgaa
    23761 acagcaacta caaccaaaac ccccaagaca atttttataa gattttcgat tgtaagttcc
    23821 attaagctct cctaaataag tttttaattt gatctaccaa cgaaatccct tcatttttca
    23881 agaaaaaaat agatataaga acaatggtta aaacaactaa tgcaattaaa atccatggca
    23941 aatattctga aataactcct tttttattcc tctttttcat attaaataca aaaaaacaga
    24001 gttaataaaa tttacccaat tacattctga aggacaatgg ttgctaaacc aaataaagct
    24061 acagaagaaa ggagagcagt taaaaaataa aatacaatcc cttttttcaa attctgtcca
    24121 attttatttg ttctttctaa tttgtcctct cctgaatcta ttgttactaa agtactagtt
    24181 aagataaaaa taacttcaat taaatatatc cctattgcta tctgaagata gtatggggga
    24241 atcattgaaa caacatcaaa atttctcata atattcaaaa tattcatatt gactgcagaa
    24301 ccaacatctg cagaagaatc taaataagtt tttaatacat taagaataga agtaatcatt
    24361 aaagctaaac cgacaacaat tcctgaaagc agaggagcta agaaagtcat attactcttc
    24421 atttcagaaa caatctctgc taaaatatct ttcaatcttt ctgttatttt ttgaatattt
    24481 ttaacatact ctgatatact cattaaacta acagcagcta tatcaagtcc ttttttagaa
    24541 gactcaacta aaaccctcat actagtagca attaaatcag atggataaaa aagaataact
    24601 cccctacgcg aatcaaatat cgctttctca acactcatac ccatctgttg aatattataa
    24661 ttaactctct taaaaaactc ccctgtaacc aaaccctttg aagaattagc aacttttcca
    24721 aaaactaatt ctggaggaac accatctcca agcctatttc ctaattgaaa caaagaatta
    24781 ttaaactcat tttctaactg ttttgtcttg tttctttcta agataatatt agcagttcta
    24841 tctttataag ctattgaaaa aaacaaagca acacctaatg gaaaaaataa acttaaaatc
    24901 aaggctacac ccccaaatgg acctgcaagc cccccctctt cggtcttctt aaaatcaaaa
    24961 atcattgtat ctttagctcc ttcaaaagaa aaacctaaat ccgcaagact ataatctttt
    25021 tctagaccta ataagtcagg caaaggagta tattgaaaaa ttaaaggcaa taatcctaaa
    25081 agcaaaaaag gaagacaaat taaaaatgca taaagatatg gtccttttga tttaaactct
    25141 ggataaagag ggtttttctc taaataagaa gtttcgccat atcctccagg tctcaacaac
    25201 aatatcttat ctaacataaa aaatgcaaga aaggggatta ctatggtgta taaaataaat
    25261 acatgagacc attttaccat cccagaaacc atagcagaag caagaggaat taatgttata
    25321 cctaaaattg gaagcattat agctaacata tacacatttg ttagtggcgc tttaacatca
    25381 tgtgtaaatt taagcatttt agtatagacc ccatctaaaa caacttgtat tgctttttca
    25441 agagtagaaa ttctcctagc cctatctggc tcataaagac tactttctat gagatgaaat
    25501 gcttcaataa attctgttga ataatctctc catccttcaa gataattatc taaactctct
    25561 tttatagttg aatattttcc aatttcaaca ttgtaaaata ttttcttaaa atctaatgaa
    25621 agaggatatt ggagatgttc tgaagcaaat gctattgcct tttccaaatt aggggtatgt
    25681 ctcatataaa ctacaacata taatattgct ggaaccatct gagaagatgc ttttaatctc
    25741 catttatttg caaatctggt aggataacca ttaaaataat aaaacaaaaa cattgcaatc
    25801 atcatcatta aaaaaaagaa tataaaagga aaatcagcta aagtcctttc acctataaaa
    25861 gtatatccta ctgaaaaaag aattcctatg aaaaaaacaa gaacaaaaat caccacactc
    25921 aaagttattg cctgccaagg ttcaatattc atgtgagcta tctcaatgtc tcttcttatt
    25981 ttctcctcat ctttttttga gggattcatc tttatgatac ttccaagagt attgcaccat
    26041 ctttcataag tagtgagttc tttctgagtt tcttctttga atttagtata agatttactt
    26101 tctttaaatg aaccaatatc tgttgttttt atttgacctt ctatcttatc cttatatttt
    26161 ttaagaattt cgtctactgt tggtttttta tttggctgca taatttcctc tttttcaata
    26221 tttgaaaaat gtaataaatt tcacatttcc ctttattcta atttcattaa taaaaaagaa
    26281 ttaataaatc tttggagaaa tcaataaaaa actatttatt tgaactaagg ttatcgtata
    26341 tcttttcatg ttctctgacc atctttgcga gacctaaatg ctctcttatt tcaggaatta
    26401 ttcttttttt tacgtcatat cttaagatat gtttattaga taatgcatcg tttatagcag
    26461 cagataaatt atttatgttt cgttgatggt cactagttct ttctacacca tatgcccagt
    26521 tattatccac ataaacttct tttggtcctg aaacagaact agcaattact ggagttcctt
    26581 gagataaaga ttctccaatc acttgattaa aacactctcc gtgggttggt tgtacagtta
    26641 tatctgcaat tctataaaga gcagccaatt cttttctatc tgcaatcttt cctgtatata
    26701 ttatattgtt cattccagaa agctctttta atttatctct gatatctgct ctagcgtctc
    26761 ccacaactag caacttaaga ttaccaactt tatttctcaa tttctttaag gactctgcta
    26821 aatcaaaaac cccttttctt cgagataacc ttcctgcata taaaacaaca ttatcttttt
    26881 tccctaaatt ataattctct ttaaaagaat ctgctaaaga acctatttct ttatctatag
    26941 aagggtcttt tgtgatatca atcaaatcta taccattata aactgtcttt tgattagaaa
    27001 atgtataacc tggaataatc tttttgccct cttcgtgttg agaattagaa acattaatca
    27061 caagatttgc taaatatgcc atttgtttct ttccagacat tgaagtaaaa gcccatctat
    27121 tcatccaatc tttatcatca attgaatgta ctttataatt atctaaaaat ttttgttttt
    27181 ctaaatcact taacttctga tagtcttttc cttttaaata agattcattt atattcgcaa
    27241 gatcttctga aggattagaa tgatctgtaa aaataaactt tgcgtttgaa aagtaattta
    27301 aaaaatcctt aaacttggtc tcatcttctt catgaactcc tgtacgaata ttttttgtat
    27361 gaaaatgttt aaaatcgtct gccaaatgcc aagtatgaga atgaattata ttcacatcat
    27421 caaaaaaaga aggattataa gtttttaata tttcaaataa ttctttcctg cttgtagctt
    27481 ctataaaatt tttcttgctt gaatcaaaaa atcttatttt ctttgaatca aaatcattaa
    27541 caaaataaaa aacattatat ttgtctttat tcaaattgtt agagatatta ttcacaacaa
    27601 ctggaacacc atgagtattc tcaagagtag atgtaataaa gagtatattc ttctttccat
    27661 ttttagtatt aaaatttaaa tttttttctt tgtgcgcaga tatttcctct aaataatcaa
    27721 tccctttatc agaatattta ttgacaaatc tcctaaaaaa atcgttctct gcagaatgtt
    27781 tatctaacct tggatcaaaa acgaatttat cagaaagtaa caattcttct aaattttttg
    27841 aagaaattac atgattagaa aaatcaaaaa gagaataatt ctcaaattta tctaaatgat
    27901 atcttttata atttttgtaa agataatctc tccaacgcaa attaacatca tcaacttttt
    27961 tcccagaagt atcaaaataa gatatccctc caacagcaaa taacttgccc ccttctaacc
    28021 tagatattaa atttgaaaat cctcctttat ccaacacatc cccgtttttt aaaaaagaaa
    28081 catatttaga attagaagaa agagatttta atgctccaag attatatgaa gtatatttat
    28141 taaaacctcc ctttaaatca acaattctta tatttaggga atcgttgtct ttagaatcta
    28201 tatcctgaaa actattttca tcctctatag ataaaagatt ctttttaata aattcttctt
    28261 ttcttaaact atctacttct ttagaattat ctaccaataa aattaattct tttttcttcc
    28321 caaatgatgt aattgaatca attgtcctct ttaaattttc gatgttatta tcaaaatgta
    28381 aaacatatga tgattcggga gagctagagg gataaagatt ttcttgaaca aattttctgt
    28441 cagaatacaa cttattctca aggcctttat cgtcattgga taaattaaat tctttgttaa
    28501 aaacagaatt tactccttgg gattccatat atcttccctc cttatacaaa gagtctttga
    28561 gtttttctaa tagaagaggg taatattttt caaacaatcc tccattgaaa aaataagaat
    28621 tattagactc gattctgttt atagggggtt ttttcaaatc gactttatag atatcatctt
    28681 gaggcaataa tgcttgttta gaaaaggtga aataattaat attatcttga gacaaaactg
    28741 ttttaagaag taatgaatta agagaaaata atcctttttt atctaattgg gtagataaag
    28801 acaataaatc ctcaaaagaa aagttgtttt ttagggattt gtccaaaaaa aaaatctaaa
    28861 ttatttttaa ttaattcttc tttatgagaa accaatatct tcttcaaaga gttttccacc
    28921 gtcattttaa gaaataaacc tcaatatatc tcttaaatct tcagttaaat aatcaaaaga
    28981 taatttagaa tctttactta gcttataacg attctctaaa atattataaa aatgctctat
    29041 taaaaaaaag tttaattctt ttatatcttg ttgtttgttc accagaatat tctttttatt
    29101 tattgtaata tctccttctt taaaaaaata tgatttttta taatggccat ctttaaaatg
    29161 atattttatt ccataaaatt catcattatt atagaatacc ttaaaatcaa aataagaaat
    29221 ccctcttttg aaatgcaatc tagagatgtt tccaacagaa ttatctatag aaaaattatg
    29281 agatgtggga attaaagaaa cactatactt caaattattt agtcttttat gcttgggcat
    29341 attcaattaa aaattaaaaa ctatttaaag attctcaata tccataaggg cgttctggag
    29401 ggtacctaat atgggaatct ttattcccac cagaaataac ccacgcaatt aatccaacta
    29461 ttaacacaac tgcaattata gtgcctatat tgcccccaaa aatattaatt ccaattccac
    29521 taaacaatcc tatatttctt aaaggtcccc aaacaactat gataattgca attgccacaa
    29581 ctgcaaataa aaaccatttc atccctttat tatcccaatc taaaaacatt cctcccaaaa
    29641 taagcaaaac taaaataatt gaaagagcaa cacctaacct tggaaaaata tctgcaaaga
    29701 aatatgaaac tatttcaaac tgcaatgcca ttaatgcaac tgccaaagaa attattgtga
    29761 caactgcttt attgttttta aaaacattaa caaaactcaa tattgcataa acaatcgcaa
    29821 atatcaaaag aaatggcaat gcatatgcaa aaattcctgc agcttcccac tggccaagta
    29881 aatttcctaa atacataaaa gaaataacaa aaacagctta ataaattttt tcaaatcaga
    29941 ttattctttt tttaatttct ttatttaacc attcctgcca aacaggaaaa actctctctc
    30001 ccaaaggtaa tccaacttct tgaattatct catctgagat tctatgaaac gcatgattag
    30061 acaatgtatt aaatttagat tctagcaaat cataatctga agtttttttt gctacattaa
    30121 ctaattcatt ttttattttt gatctaagaa gaatattgtc ataaactgca tcccaattac
    30181 ctgcccaacc ctttacattt gaagcaacat ctttaattat ttgactatct ccattaatta
    30241 aatcctcagt gggttccaat tcatctttct cgatattata cttcataagg tcaacaaaac
    30301 ctttttctgc tagcggatct tttgtccagt gttttcttac ttcagaaagt tgaattaatc
    30361 ttttccaagc atgtaatcca tctggagatt ttattggatt gcatacaata actgaatcag
    30421 ttgctttaaa actagttatt ggaacttcca aatcattaac tactctatca aaaacaccat
    30481 aaggagatgc tccatggatt gtacctgcaa caacatttgc caatgctcca actctcattg
    30541 cttcataaag tgctttagct tcaacactcc taacttcccc tataatcaaa caactatctc
    30601 ctaatctcaa acttgttcta ataccatctt ctgctgatac ttctgtagtt gtttttaaga
    30661 gagcagaacg aactttcatt cttaaaatat cataattaag ttttcttaaa gcttcaacag
    30721 gtaattctaa agaatcttca ataactatta ttctatgttt aggaacaatc tcaagcataa
    30781 gacttcctaa aagagaagtt ttaccagaac tcctagttcc agcaaccaac aatgttcgag
    30841 caccatctat taaaaaacta agtaaccctg caccaaaagg attaatcatc ttgttctgaa
    30901 taaaaagagg caatgtccaa ggactctctc tatgtcttct tatagaataa gctaatccat
    30961 cgcaagataa gggttgttga acaattgaaa ttcttgctct aagagttcct atttctaact
    31021 gagtatctaa tatgggatta gcttcatcca agggtctacc agaaatcatt ctaaattttg
    31081 ctgcccatga atcagcatct tcctggctag gcaaaatatt tgttgtgcat tgatcatatt
    31141 cctgatgtct tacaaaaatt ggagtctgtg gaataggtgc atttaaagaa atatcctgaa
    31201 gatttttatc ttggagtaaa acctctacaa ttccaaatcc aattgtatgc ctaacgagaa
    31261 tagaagacaa tttattcaaa gatgaataat ctaattttat gtccctactt tgagataaat
    31321 ctcttataag gtctttagag atattcataa acacttgcct aactctttct gtatctgtaa
    31381 attcttctgc tttaggttga tgttctatta aaacccctct tgccaaatta agaattatac
    31441 tttgatcttc agtcagagta ttttcagggg gaactaaatg ataaataagc ttagattcat
    31501 tcttttttct aagtatagtt acattggatt catcatcttc tccattagag attttatatt
    31561 gagaaattat ttctgcatct ctaggcaaat ctgaaaccaa ccgggtaaat gtaaaattag
    31621 caataacatc tggtctgaaa agtttatggt atatttctct atctcctctt ttatattggg
    31681 tagaatactg ctgaaatgtt tgaataaaat tagttttaga aaaattttct aaaatttttt
    31741 ctactaaagc aagatagttt ttttggtcta atttcaagac cccatcaagt ttatccaaga
    31801 ttatctttcc ttctaagatt atctttctaa gttgaaaata tgctgaaagt ggatcttttt
    31861 taagaagata taaaaaagaa aacatttcat tatatcttct tggaaagaaa tcaggattag
    31921 atgtgattaa cttctcttga ctaagtattt tttcttgttt gacaagataa acatagaatt
    31981 gagctatttc taataaataa cctgtttctt taaaatcata attgtaattt ttttgttgaa
    32041 caaaaacaac tcttgaaact tgagggtttt caattaaaga atcaattgtt ctttccataa
    32101 cttctggatg ttctgctagg ctagggacat atggagcccc catataatta atataaagaa
    32161 catcttctcc tccttctctc ttgacttcat aggaatacag ctcatcctct tttccaaatt
    32221 gcatgaaaga aaaaatagtc tatagtttaa aatattttgt ataatccaaa caaatttaaa
    32281 gtagatttac ttctagcaaa tatggctgaa gagggatatg aaaatcagta ttttacagag
    32341 gttagcacaa aaataagaga tttggaagat aaacaaagaa taatcaaaga cagagtactt
    32401 ttgataggac aaaacttaat tgaagtaaaa aaagaaatgc attctacatt cttaaatata
    32461 aaaaaagaaa tagaaaattt aaagatagat gttgaaagat taaaatcttt ttttgaaaca
    32521 gcatcaggag agttttcaaa atttgcaaaa aaagaggaat tagaaatact ctcaaaacaa
    32581 gcaaaaatgt ttcaaccttt agaattcgtc acaagaaaag atttagaaaa cttaaaaaac
    32641 aaataaacac taaataaaaa atgggaataa tagaagatat aacaaatatg aaaaaccagg
    32701 gaatgcagga aaatgaaatt tatagaaaat tacaagaaaa aggagtttct cctaaagcgg
    32761 ttgaagatgc attcaatcaa ataaaaataa aaaatgcagt ttctgcagaa tatgctggag
    32821 aagaaggaat ggaaccttca attatgaaaa atgaactcca acctccaact cctcaagcgc
    32881 ccccattata cgctccaaaa acccaagaaa tagagcatga cccacgagaa ttttattcgc
    32941 cacaaccaat ggtacctcaa ttcaatgaaa atatacaacc tccaatgtat aatgcaggtc
    33001 ctgaatatgg acaagaacaa tatattgctc aagaaggata tgtagaaaac cccatgggag
    33061 gaggttatga tacagatact attatagaaa ttacagaaca agtattttca gaaaaaataa
    33121 aaaaagaaca aaaacagata gaatctctaa atgaatttgc aacacttgtt gaaacaagaa
    33181 taagtaatta taatgaaaga ataaaaagaa tagagagtat aattgacaaa ttacaaatag
    33241 caatattaga aaaaataggt tcatatggaa aaaatataga gtcaataaaa aatgaaatgg
    33301 gaatgatgca agaatctttt gaaaaaatgg tcccaatact tcacgaaaaa tctgaagaga
    33361 aaaaaacagt aaaaaaaaga aaataatctt ttaataatta aattgtctta ttaaaaatta
    33421 aatttaaaat ttatctagta ggatttctta ctggagcatc tccaacacta atctttgtat
    33481 tcccattaat aacccacgca attaatccga ctattactac aactgcaatt atagttccta
    33541 tattccctcc aaaaatatta aatccaattc cactaaacaa ccctatattt cttaaaggtc
    33601 cccagacaac tgcaataact gcaattgcca caactgcaaa taaaaaccat ttcattccct
    33661 tattatccca atctaaaaac attcctccca aaataagcaa aaccaaaata attgaaagag
    33721 caacacctaa ccttggaaaa atatctgcaa agaaatatga aactatttca aactgcaatg
    33781 ccattaatgc aactgccaaa gaaattattg tgacaactgc tttattgttt ttaaaaacat
    33841 taacaaaact caatattgca taaacaatcg caaatatcaa aagaaatggc aatgcatatg
    33901 caaaaattcc tgcagcttcc cactggccaa gtaaatcacc aattgtcccg cctccaaaaa
    33961 aaccataaga agctaaaaca atcccctcat ttatcattct ataaatttat ctaacattgt
    34021 ttaaatatgt ttctactatc ccttagattt tcctgatgct aaaaccacaa caagagcaat
    34081 aacaaccaaa actatgaaaa ccgtattggt ccaaaattga ttgctccaag aagaattgaa
    34141 taatctatca aaaaagctag ctgtgtctac tccagcagcc caaagaactg caattaaagt
    34201 agttataaca acaaaaattc ctattgccca tttcaatcct ttaggagcat tcttaaatac
    34261 atttaatccc tcttctccca ttataaatcc ccacaaaaga agaaccacaa aaataactac
    34321 taatgctatt gccaagaaca atattaaatt actagtcact tctttaggaa atactgcaga
    34381 aacaaaaata aaactaatta caaaagaaat taacgcatta atctgtttag tacctgaacc
    34441 aaaaacctta gtcttctcta aaactgcaaa aagtataaag aacataagta aaaaggggta
    34501 tacaaactta gttaaaagtg aattctgtaa aaaagttact tctgtcatta cctcttttta
    34561 ctccctttaa cttacacttc ttcaaaccct gtatcttggc ttggtaaagc tgatttactt
    34621 attatgaaaa catcactaat agacctaaca aattgatgag ggattatcac tcctctagct
    34681 ccccccatta aagaaaggat atttccacca actcttatcc tccatccatc aattttgttt
    34741 tcaaagagat ttgcttcttc cacttctcca aaaaattctc ccgaatcagt aaataccctt
    34801 ttacctaaaa cctctgaaat ttttttgatt ttcatattag gaaatacaat agtttattta
    34861 aatacttttc catgatggat tgaatattgt tgtaaaatag gataacttgt gaatatcata
    34921 agatttataa aaaaaaatat cttaaaaaaa acatggcaaa gggaaaagag attgggtatg
    34981 tgatactcat aacaataata accgcattcg caatatcatt aacaaaagaa ttgtccataa
    35041 atctaaatct cctgctcctt tctttacttg cagtatttat attactaaca gtaaatattt
    35101 taataaagaa aaccgctgca tattttttag aatctgaaat agaaataaaa ttatgggaaa
    35161 taaaaagatt tggttttaag acaggacaac atttcaaaaa agcatttcca ataggagctt
    35221 ttatgccaat tatattcaaa atactcctat taccattaaa cagctttgtc tggatggctt
    35281 cattagtttt tgatgtaaag gcaaagtcat atagggcggc aaaaagacac ggtatatatt
    35341 ctttttcaga aatgacagaa gaacacattg gaaaaatagc tgcaacagga ataattgcta
    35401 atctcttcct agcattaatc ttttatttca taggaggaaa ctttggagtt ttatttgcaa
    35461 agttaaatat ctggtttgct ttctttaata tgattccaat atctgattta gatggaaata
    35521 aaatattctt tggtaatttg gttctttgga gtttccttgc aataattact ttaattgcag
    35581 ttggatatac tttcttttta atataaaatt ttaatataaa aagaatttat aaaataaata
    35641 aaagttaaag caataatgga atttttagaa aaaataaaac caagaaaata tcaacaagag
    35701 atatttgaaa catgtaaaga caaaaattgt cttgttgttc ttccaactgg gctgggtaaa
    35761 acattaatcg cattaatgtt aacgatagaa agaatgaaat tgtttccagg agaaaaggtc
    35821 ttatttcttg caccaacaaa acctctagca gaacagcatt taaaatactt caagagaaac
    35881 cttccagaat tatttggaga aatgcagtta ttcactggaa gtgttaatac tgaaaaaaga
    35941 aaaaaaatat ggcaaacagc agatatcatt ttttcaactc ctcaatgtat tgcaaacgat
    36001 ttaaaaaaaa gaatgtattc tttaaaggat gtgtgtcttt taatagaaga tgaagcccat
    36061 agatgcataa aaaactatga ttacagatat attgcagaag aatacaaaaa ccagtccaat
    36121 catccaagga taattggatt aactgcttct ccgggaagtg aaaaatcaaa aataaaggaa
    36181 atctgtaaaa acctctcaat agaagaggtt gaattgcgta caagagatag ttctgatgtc
    36241 aaagaacatc tacaagaatt agaatttgaa aaaataatgt tagattttcc agcagaattt
    36301 caagatatga ggcatatctt attaaaacta tttggggaat atgtttcaga gctaagagaa
    36361 agaaaggtgc tttttggacc agcttcaaaa acagatttaa ttaatcttca aaaaaaaata
    36421 tcctctacta tctcctcaaa ttataagaat ttcaactata tgagaggggc aagtgcgtgt
    36481 gcacaagcaa taaaaataca acatgcatta gagttgttag aaactcaaac attagaagga
    36541 ttttataaat acctcaaagg attatttgac caagcctcaa aaaaacaaag taagggggta
    36601 ataaaattgg tatcaaaacc agaatttaat tttatttatt ctcaggcaaa tgaacttcta
    36661 gcaaaaaaag tagagcatcc aaaaatgcaa gaaataataa gcataataaa acgagaaaga
    36721 gaattcaaca aaaatctaaa gatgataata ttcacccaat tccgagagac tgcaggaaac
    36781 atatctagga gaataaatga gatagaagga gtaaaatcta aagtgttcat aggacaagca
    36841 aaaaaagatt ctttgggagg gttaaaccaa aaacaacaaa aaaagataat agaagaattt
    36901 tctgaaggaa aaataaatgt tttatgtgca acatgtattg ctgaagaagg attagacata
    36961 ccagaagtaa atatagttgt gttttatgaa ccaatacctt ctgcaataag gacaatacaa
    37021 agagcaggaa gaacagctag gttgatgaaa ggtaaattaa ttatactaat aacaaaaaaa
    37081 acccgtgacg aagcattttt ttatgttgca aaatcaagag agaaaaaaat gaaaacagct
    37141 atagagagta taaaagaaga tttatctaaa gaaggtccaa ttaaaacaat taaaacagaa
    37201 gtacaaaaga aattagaatg aaaacagtaa aatctcctat aatgttagat atattttcta
    37261 aaagaaataa aataaaagaa aaaaaacaag aagataaagc agaaaaaata atcatagatt
    37321 atagagaaaa aaattctttg gtagcatcct acataataaa agaaggatta gaagtggaat
    37381 tcaaagaatt gaaaatagga gattatatag taatggacac cataatagaa aggaaaacag
    37441 tttctgattt tatctcctca atgattaatc aaagactatt aaagcaaatt gaagaaataa
    37501 aacaatatga aaataaatta attatagttg aaggaatatc tgaaaaagaa ttgtatggag
    37561 aagaaaactc tggagtaaat ccaaatgcaa tcaggggatt tttactttca atagccctca
    37621 aacataaaat tcctttaatc ttttctaaaa attcagaaga tactgcaaaa tttataacaa
    37681 tattatctaa aaagaaggat aaagaaataa atctaaatgc aaaaaagaaa acactgggga
    37741 aaaaagaaca gttacagttt ataattgaag gttttccagg cattggtcca aaaaaggcaa
    37801 gggcattgtt agaaaaattc gggtccatcc aaaacatcat tcttgcaccg actgaagaac
    37861 taagagagat attaggagtt aaatcagaac agataagaga gataattgaa agaaaatatt
    37921 aaacaaccct ctctcagatt aattctgaaa taatttttgc tccaagtgtt aaagttaaat
    37981 gttccttatc ttttaatgga ttaatctcca ccaaatcaac tgctctaaga ttttttattt
    38041 tattcaatct agaaattagg taaataaact ctctacttgt taaaccccct atctctggat
    38101 tagcagtagc aggagcaaac aaagggtcaa tcacatcaat atctactgac aaatacaaat
    38161 ctttcttatc tgagaattcc ataattattt cacaggtttc ttcaatatcc tctaaaagag
    38221 agtttaaaga caaagttttt attgaattat ctttcaaaaa cttaaattct tccttgctca
    38281 tatttctaat ccctacaata aggatattct tttttggaaa accatcttct attaattttc
    38341 ttaaccattc ttcatttttt ggaaaatcct gactagaatt tagaaagggt ttgcaatctg
    38401 gatgtgcatc aaataaaatt aaacagggtt ctttacctct ctttttagaa gaatccaaaa
    38461 atgccttgca taaagaataa ctaatagaat ggtctcctcc taaaaagatt gttttaggtt
    38521 tactttcaaa gatttcaaaa ctatttttat aaataagatg attagttaat tttaaattag
    38581 aattatctaa gtgtatttcc tctaaatcca aattagttat atcaacaaca tttcctgatt
    38641 cgtttacaca accctctttt tttaaaattt tcaaaatttc gtttccagat ttttcacatc
    38701 cagaagtatc tgaatttccg tttattccgg gtactttaac tacaaacata acaatacaga
    38761 ataaattctc tttttaagat tatcttaaat gctatttagg tatgagtgca aaaaaactat
    38821 aatccttaac tacctttttt gctaaatttt taacgtcttc taatttaacc ttagaaattt
    38881 ctttttcaaa attataaaat tcttttgcat tacctcttac ctcgtgtgcc aaaagattca
    38941 ccatttgatc ttgagaatct tccatagaaa tttgatagtt tccaattaac tgttctttaa
    39001 tttctttaag atctttttca attaatgaag ttgaaacttt ttgaaattct tctataatta
    39061 acccttttac tttttctaca ttctctttca ttgttccaac ataaataata ttatatgcaa
    39121 aagttttgtt tatatttaca tcgcctttaa ctgcataagc caagtttctt ttttctctta
    39181 tttcactaaa taatctagaa gacattcctc cagccattaa ggcatttaaa actatggcag
    39241 aataactttt tttatctcct gctagaggaa catgataagc aaaaacaaca tttgcttggt
    39301 ctatcccaac tcttttttct gtcctaattc ctctctctaa tccaaacttt tccactttaa
    39361 ttttagagtt tgagttgtca aaatttcttt ctgcaaattt taccaactct ttaaaatctg
    39421 catctccaac tacgcataaa ataagattat ttgaagtata aacttcttta aattttttaa
    39481 ccatcttttc tctggaaata gaattcattg tctcaaatgt tcctgcaaga tttattttca
    39541 tagtcccatt atacattaac ttttgtattt catccataga atgtaatcgt ggattatcat
    39601 gatacatttt tatttcttca aaaattactt ttctttcttt ttctaattct ttttcgtcaa
    39661 ataatggatt tttaaccata tccgataaaa catctaaagc acatttcaaa ttttttgaag
    39721 gcattttaca ccaataagca gtaatttcat cagaagtaaa tccattaagg tctcctcctt
    39781 ttttctctat ttcctctgca attttcttgg catttcttga aggagttcct ttatataaca
    39841 tgtgttcaat aaaatgagaa atcccttttt cctcttcttt ttcatttacc cctccattcc
    39901 ttactgcaaa tgcaacacta actatgggaa gatttctttt ttcaagaatc acagtcatgc
    39961 catttttcag cacctttcta taaaaactaa ctttcatact ttaaaaaaga aaagaggcta
    40021 tttaaaccta cttctaaact taatgtatct ttcatttcct atttcaaaat cttctaaaac
    40081 ttcaaagttt tcttctataa actttaaaat ccacttacga ttaaccttaa atctctctct
    40141 tttaactaaa cttctagttg caaaactaat aacaacaaaa tcaactaaag gagtcaattc
    40201 tttaatcaaa gattttgaat aattgtcttg tagcatctcc aaagaatcta taaccttaaa
    40261 caaaaataaa atttttaatc ccttaacctt gaggattaat ccctttatct tatctaaatt
    40321 gaataaactt tcttgaataa cttttccttt aatattctcc ctattaaaat aatgatttgt
    40381 taagttaaca agctggttca ttccttcaac ccccagataa tttatatttt ttggaaaata
    40441 tttgtaacta aatccattta cccctgctcc caagtcaata actgttaaat ctactctctt
    40501 gttcacctta tctaaactag aatctgagaa aatcctatga taaacttctg gataaaactt
    40561 caatctctct ctggttgaaa tatgcttatt taatatccat tcgcaatctt tatcttttat
    40621 attcaacaac tttccactag taaaaacact aaaaaccttt ctaagcaaat ctctggttaa
    40681 tttaactttt tcctcgtctg aataatgagg cctattaaat ttattaaatg ccaattcaac
    40741 atcctgttta ggtaattcag aaaaatcgtt tttggttgtt atctttttta ttaattcctc
    40801 tctattcata aattatttaa gctcttcgac tttaaaagtt atatgccaat aaatgctcat
    40861 ccagaatata ttgcagcaga aggagaatat cttcgtgcaa agtcggtaga agaaaaaatt
    40921 gaaaaattaa aaaaaatgat ttcttatgct ccttctcaca aaggagggga aaacctaaga
    40981 gcgcaattaa agacaagatt aaaaaagctc ttagaagaaa aggaaaaaag taaaaaatct
    41041 ggaaagtctt caagaacagg aataaaaaaa gaagatttac aagcggttat aatcggcaaa
    41101 tctaattccg gaaaatcctc tgttttaaca gaattaacta atgcaaaggc agaggtttca
    41161 cacactcatc tttcaacaac tactcccaaa atagggatga tgaattatca aggggcaaat
    41221 atccaaataa tagaaaatcc ggcaatagaa tcagaatatt atgacaaagg cattacaaat
    41281 actacagaca ctttaattat tataataaac tctatccaag acctaaaaga aatagaaaca
    41341 atgattggaa acatacaaaa aaacaaaata gttttattta ataaatcaga cttattgaac
    41401 ttagaagaaa aaagaaaaat ttcttcaact cttcagtcaa aaaaatacaa ttttgtgtta
    41461 ttttcctcta taagcaaaga aggaatagaa ttattaaaag aaaaattatt ttcatcattc
    41521 aacaaaataa gagtatatac taaagaacct ggaaaagaaa aaagcaaaaa tccgataatt
    41581 cttaaacctg gagctactac cagagaagtg gctgaaaaaa tattaaaggg attttccgaa
    41641 aaagtaaaag agacaaaaat atgggggcca tcaagtaaat ttcctggaca aattgttgga
    41701 cttaaccata aactaagaga tttggatgtt gttgaattta aaacaaaata aatttaaaaa
    41761 cattatattt aaatacaaaa ttaaactaaa aataataaag tttaaattaa aataagaaag
    41821 tgaaaaagaa aacaattggg atactctctt gctcaagaga aatagaaaaa gagggaaaac
    41881 tattctttga gatagcaaag aaaaaaggag taaaagtaat tctcataaac ctttcagata
    41941 aatttgatat aaaatcattt aaaagaaaag tcaaacaatg tgacataatt tattgttcag
    42001 tgtgggatga tttttcagtt gagcttacta aaacaataga agaatgggga ataaaaataa
    42061 tagagtcatc aaaatcatat tactgtacag aagacaaatg gatgttttat ataaaatgta
    42121 aaaagcacaa tgttcctacc ccagaaacaa tacttttatc tgaaaatata aataatgcca
    42181 aaagtgaatt aaaacaattt ggaagatggc cagttattct caagagaata aatggaacaa
    42241 tgggggaata tgttgagaaa gctagaggaa tggctgaagc agaacaaata ataaaaaaat
    42301 tctggaaaaa aggaagtgaa aaattaccaa taatagctca agaatttata gaatctccaa
    42361 gttatagagt aactgtaata ggaaataaga tagttcagac agcattaaaa aagaataatg
    42421 caggatggaa agcaacagga gtatatgcaa aaagatttga accttttgac atagacaaaa
    42481 aattagaaag aataatcaaa aaaacagtta aatttttgga cattaaaatt tgtgggatag
    42541 atgtattaaa aaaaggagga aaatggttat tattagaagt aaacggacag ccatctttgg
    42601 acttttttga agaggaacat gaaagaataa tagaagaagt tttaaactta ctcataaaag
    42661 ctgcagaaga aaaataaaat aaattcaacc aaatatagtt tccaatatct taaattctta
    42721 aaaataatct cctgctcaat taggaatgat tattgagata ataattgcaa taattcttgg
    42781 aattgttgtg ggaattttca caggcttatt ccctggaata catattaatc ttgtaggaac
    42841 atttttagtg actctctcct cttctttctc taatcattcg tttatgatat atctggttgt
    42901 atttatctcg tctcttgcaa tagcccacac ttttattgat ttcattccaa gtatttttct
    42961 tggttgtcca gacacagata cagaattagc tgtacttcct ggacacgaaa tgctaaagca
    43021 aggaaaagga cacgaagcag taatgagaac tgcttatggg ggattaagcg gagttattct
    43081 tttagttctt ctttttttac cgctctcgtt tttattgtct aaaacttatc ctacaataca
    43141 aaaagtaatt ccatacatcc taatcttagt ttgtataatt atgattttct cagaaaaaaa
    43201 taaactccag gcattccttg taatgattat gactggagtt cttggatacc ttgtattaga
    43261 tttaaattta aaagaacctc tcttacctct tttaagtgga ttgtttggta gctctatgtt
    43321 attgttgagt ataaaacaaa aaacaaaaat acccaaacaa aaaactaatt tcaaaaagat
    43381 aaaattaaaa aacatgatgc gtcctctatc tggagcaata atcgcctctc ctttttgtgg
    43441 ttttcttcca ggattaggag gaggacaagc agcagttatt ggaaaccaaa tatctagaac
    43501 aaacgaaaaa gattttctta tacttttagg aatagtaaat gtatttgtta tgggactttc
    43561 cttcgtgtct ctatatagta tatctagaac ccgtacaggt gcagctgcag cagtgcaatc
    43621 tctccttggg caagtaacct ttcaaagttt aatcttaatt attattgttt gtttaatttc
    43681 aggaataatt tccttttttc ttacaaaaaa attatccata ttgtttataa aatatataga
    43741 aaaaatagat tacaataaac tctctaaatg gactatttta tttcttagtg ttttaatttt
    43801 aattatctct gggattcttg gattatttgt atttggaata tctacattag taggattgta
    43861 ttgtattcaa ctcaatgtta aaaaaacaca gatgatggga tgtttattaa ttcctacaat
    43921 tttattatat cttctttaat gaaaattaaa accatttgct tatgttctct tgtttttcta
    43981 attctctccc aaaaatactc tcaacatatc tttttacaag ttctaaattc tgttgcatat
    44041 aattagaaag attatatttt ttagccaaat ccaaggcagg ttctaagtat ttctttattc
    44101 ctccctcatg aattgtaaaa ataagtttgc ctctacaaaa aggacaaacc ccgtttaggg
    44161 gaactcttct taaaatttca ttacatccaa cacacctaaa ttcttgcata gaaaattttc
    44221 tgagattacc tctcatatct cttataaaat gtctatctat aattaatctt gcagtatctg
    44281 aagtatccac tgacctcaac ttctcgacca aattcatttg atgattcacc ttatctttca
    44341 ttgtcaataa acgtttatat gaagagcaca aaatcccttc attgaaatta tctgtatcat
    44401 gtgtaaaacc tgctctcaaa aaaggattct ctcctttttt taaaatctct cgtacagtat
    44461 ttactttaac ttctgaagaa tgaactcctt tttcagcagc acgatataaa tcaagaggat
    44521 aattcattaa aaattcaaaa tccagtattt gatcatccac ctctccagca tctatttttg
    44581 catttaaaac aaggggagca tcttgtgttc ctcctctatg acttggtaaa aattctcttg
    44641 aaaaattaag taaaacatct ccaagaagca taattgctgc ttcatctcca tcacaatctc
    44701 ttcttacagc agcatgcata tatggactag caaataaacc ctgtgtattt gaaaatccta
    44761 caaacctaca aataacccct gcacaattat gaggagccat acaaacaccc aatttaccaa
    44821 ttaaatcttc tttattttgg acattataaa ttttttcaag tccataaacc ttttccaact
    44881 cctcgtcgac aaaacgagaa accttaaaaa aaacatcatc tgctctttcg tctaaagagt
    44941 caaaggaact aggtaataaa atatcatggg gcattaattc caaaatttgg tcttctcttt
    45001 ccaattcttc tccaaaaata tctttatgat aacctatttc acgaagtttt tttacactca
    45061 cataagcctc tttaggttta aagtaaagta aaggtaattc tgttgcatca aaacgaatag
    45121 ttccgtcctt atttacttgt aaatcatatt ttgctcttaa aattccctta gataagtcct
    45181 ctacagcatg atttgcagaa gaagtgcctc taactccttt aatcaaaaga ggagcttctt
    45241 ccttagatac tcccaatctt tttaatgcat gttcataata atgatttata tctattgatt
    45301 tattacaata tcttactcct tctctagaag aattttcatt agatttacta aaaagttttt
    45361 cttttgtttc cataaaataa tacatcggct tagtaattaa attacattct tcacaagtta
    45421 gatagattgt ttctttttga catgaatcac aataataaat aggaaagctc ccccaaactt
    45481 ttccttcttt acaagctgct tgtaaactcc tcaaccttcc tccttctttc ccaacaggaa
    45541 ataaaacatt cggacttcca acaagttttc ttagtttagc tttttctggt ctacccatac
    45601 gcgccccaat aaattcccct gctttatctt taattaaata tttagaaaaa gaattaacaa
    45661 tctccaaaac agatttatct ttatttaaat tataaaagtc aaaaatttcc ttaaaattaa
    45721 ttttatttcc atcaacttta tttaattcaa aaccgaggtt aaataaaaga gccctactta
    45781 tatcctctgt taaaacaaca ttttctacag tgacttggtg ttcaacaccc aaaatctcca
    45841 atgctctctt tcctaatgaa aatttttctc tttcagattt attataaggt aaaattattt
    45901 tcttttcaat tcttgcattt tccaaccaaa taagtaaatc taatagattt tctcttgaaa
    45961 tttgagtcca ataaaaaata tatttaggat gaagaggtag agaatatttt tcgcttaatt
    46021 caatagcatc atcaaaagaa acagaaaaag gattaatgtt cttcaaagaa ttttcttttt
    46081 caattaattc caaatgccac cattcttcaa catatcctgg cttaataaga ttagcattcc
    46141 tgtttgctaa gtcactaaaa ggaaaaagaa tatctccaag ataaataatc tcatctatgt
    46201 ctggataaat agattttgtt tcttctatag aagataattt ttttacactt ccattgaata
    46261 attttacaat aggaccatca atactatcac atacagtaac tgcgcaacct tttgtgggtt
    46321 tttctatctt gagctgtgtt ccaattgcaa taaaaccgtc tgttacagcc atagttgcag
    46381 gatttaaaga agttgcacta aaaccagata ctcttcctct tccatatcta aatctaaatc
    46441 ctccagattt tgaaggatgt ccaaaaatag gtcttccagc aaccaaatct ttaatatatg
    46501 tgggagaacc atctgctttt cctctatctc ttttttcatg tatcttaaca tattcctcta
    46561 aaaaatcaaa accagtagat tgtatcccat ttttttttgc cctgccaagc aatctaaatc
    46621 cttttgctgc tttttgagca agcccttctc caataataag acaaaaacca cttctcagat
    46681 aatttgtgtc cactctttct aaatttttat aatttggaac ttcaattgtt tcagaagcat
    46741 ctcctgcaat ttgtatggga agatgttctg ctaaaaaaag tgcttcttct tcagttggca
    46801 tatactgtaa attagtcacc ctatcatgaa aatgctccaa ttcagcataa actcttttta
    46861 tttcatctgg tgtaggatca tattttgcaa aaccaaaaat ttctcttaaa taatctataa
    46921 caatcaaaat aacacaactc gcagtagttc cagcacttct aatcgggcca gaaaaactcg
    46981 cttcaaggta ctcttttccg tcacgagttt ttcttatttt taatcctgta aatccttcta
    47041 tgggacttga aaccactcct aatgtagtat aagcaaatgc tatccttatt ccggcatcca
    47101 tagcttgtaa taaattttca aatttgcaga atttttgttt agctacttcc tctgcaattt
    47161 taaataaaat agtcacatct agtttaccat actccttttc taactctaaa atcctttctg
    47221 caatacctgc tcccttcatt tgaggataaa ttgtagatat taatccaact accttttcag
    47281 ccatactcat agctaaagga atttcaactt catgaacagg atcataacct ttagaacgag
    47341 ctaaattagc aacagaataa actttatcca cttctttttt caaattttca aaataaattt
    47401 tagtatccat tttaaactgg aattattttt actttcttaa cctccttatt tctttctttt
    47461 tcatattcta ctgttttttt ataatcctta caaatttcac ttaaacaaac aaaacctcca
    47521 ttaactttaa tttcagggtc aacatatcta aatttctttt ttaattctat accttcatta
    47581 tcttcaataa tagaaaagcc attctttaac atacttaaat taagtaagat aaattcatta
    47641 tttgaattat ttaaaatttc taaaataggt ttttcagttc cattaaaaaa actaaaatca
    47701 attagatttt tgtttaaggc ctctttcaaa gtttcagaca agatatgata cctgcttcgc
    47761 atttcgtctc cagcccaact agatttttga agtcccatat atgaatctcc aaacaatttc
    47821 gctggcttaa aattcttaaa cataatttga ttatcataaa cacataaatc atttaatatt
    47881 ttatctgtta attctttacc tttttctatc cttaattgac gtaaagaata atctaatcta
    47941 tctacacaaa gttttggagc atctctctct agaagtttga aattatctaa atttgaaata
    48001 aaataaggat ttagtccatg ttggtccaat atatcaaatg catctgattt ttctaaaaat
    48061 tcttgaaata ttttatcttg gtaatcttct ttagtaggat ctccaacaac ccaatctata
    48121 acatgagaaa aaggagtgtg ggagatatca tgtaataacc ctgcaacctg ttcctctaat
    48181 gttgcaccta actttttcaa aagtatcatg gtgcctaaag aatgttcata tctggaaaaa
    48241 ccaggaaaag aaagataagt atcaggcatc cctaattgag aaatatcttt taatctttta
    48301 agagaattag aattaattaa atctaaaata acattttctt ctatcttctg aactccataa
    48361 actctatcct taatttccat ttgaattggt tgtttctaaa gaaggcaatt tattttcttg
    48421 aacaggttca ttatcttcaa aatccaatat tttaactttt cttgtcttta aattaaaaat
    48481 aggaacttta caatgatcgg gaatatttcc aaatttttct tgataaggag tcatttcttc
    48541 ccaacatgaa acagaaacaa ccaagatatt attgtaatat gtaactccac atttatgtgt
    48601 gtgagcagat acaaatatat ccgggatatt tcttattaaa agaccatctt tctctaaagg
    48661 aaaatatttt gtagaagcat gagttggagc aagatgtcga tttttaagaa gatacttcat
    48721 aatttcttct ggacaattca ttgctttctt ttgaataagc gaggggatat tatttgcata
    48781 ataaggataa gaaaaaccat gataagttaa aacattaaat ccagaaaaat tttctctttt
    48841 cccaatatta acataacaag gattaggtgt aataaaaaca ttttccatct catataatgg
    48901 ccaagaatat ttttgatcta aaaaaggctg gggctccata agtctaacac cgtcatgatt
    48961 tccaggagaa ataattattt ttacatcttt tcttatctta cttaaaagtt ctgctaatcc
    49021 aaaaaattgt tcttctaaat ctaatatctc taaatctttc tcttgattag gataatttcc
    49081 aacaccagtc acaatatcgc caactaaaaa gaggtacttt atcttttttg cttctttatc
    49141 ttcgtctaat tctccattta aataatttat aaatttaata aaattatctc tcataaatct
    49201 tttacttcca aaatgtaaat cgccaataaa tgcagcatat tcatcattta aagaaaattt
    49261 tctttcagga agagcagaat caggataaat tatatcatct gcaaaaaatc tctcttcatt
    49321 acctgaacct ttaaatccaa taacagaatc caatgcaaca ttttctgctt tttcatacag
    49381 ctctttttta tccttgttta ttattatttt aattaatcca gtagtatctt caacatcaaa
    49441 gataatattt ctattcttag taaaagactt ttttgataca atcccaatta tagatatctt
    49501 ctgtttatta ccaaatattt tacttataga aattggatta tctaattctg gccttgattg
    49561 caaaattccc ctcatttcaa caaacctgct cctaaaataa gacacaaaat cctgaacctc
    49621 taatttttta tttacaacag gaaaagaaga taaaattaaa acctctccag aagaatcaga
    49681 agattctgaa gaatcagaat aaactaaaga actagaagaa gaatttgatt tgaccgagtt
    49741 aattgaaagg gaattttttt catctactat agattctttc tctcttgaaa tttctatact
    49801 cagacccaat ttaattttaa aattttctaa ttctttttgt gatttttgag gcaattcata
    49861 aaaaaattca tttactttct ctttgtgttc ataaaagatg tttttagtta tgatattctg
    49921 acgagtataa tttttcagtt tatcaatgat taatttaaca gaatttatat cctttgctcc
    49981 attaaaaagg ttcaaaacat ctttatccac taataaacca ttctctaaac aaaccttcaa
    50041 aagttgcttt gaatccattt ttgaatataa gtttaaacat aattaagaga gttttctatt
    50101 atttctttag ctttgttggt aatttcttta gatattgcca tctttatttc tctcattctt
    50161 cctcctcttc cttttgaaat tactcttaca ttaattatgc ccaacatatc taattcttga
    50221 ataatctccc caactcttct ttgtgttaaa atctccaatc tatttttaag acaaagttgc
    50281 tgataaaaat cataaacctc tcctgtaaaa acaggtaaat catttttttg gtgttctaaa
    50341 agataaataa ttgaacctaa aaccaactga aattgctttg gttcagaaga gatagtttct
    50401 aaaattttat ctttttctat tttttcatta gcattatcta tatattttaa taaaattttc
    50461 ttagaattat ctctttctgc taattctcct gctactctta gtaaatccaa tgctctcctt
    50521 gcatcaccat gttcttttgc tgccagtgca gcacatttat tgataacccc ttcctgtaaa
    50581 accccttctt taaatgccaa ttctgctctc tgattaagaa tatcctgtat ttggagtgca
    50641 ttatatggat gaaaaagaat ttcttcttca gacaaagaac ttctaactct agggtccaac
    50701 tcttctaaaa atcttaaatc attacttatt ccaacaagac atatttgaga tttagataat
    50761 tctgagttta atctaacaag gctatataaa aaatcacttg aaattttctt aacagtttga
    50821 tctatttcat ctaaaataag cacaaccaaa ttcttttcag aatcaattgt ttctataaat
    50881 ttattataaa ttgcttgtgt tggtaaacct gttaaaggaa catctccccc cattttttta
    50941 actaattcag ctaaaatcct atattctgta tcagaaactt tcttcatctt acaatttaaa
    51001 tattcaattt taagtttaaa atcagcatct ttcttaaccc tcttatacaa ttcttcctga
    51061 acaaatctca ttgcaagagt tttccctgtt cctgtctttc catataaaaa aagattactg
    51121 gttttttcac ctcttaaaac aggggctaaa atagaagcta tttgctttat ctgttcttct
    51181 ctatggggga tttcactagg agaataattt gattgcaaaa tagttttatc tttaaagata
    51241 ttatttttat caaagctatc aaatatctta tctatatcta acttcatttt aattaattta
    51301 agctaagact cacacgtcta tttcctgtgg aaactttatt cctccttaag ttatcgatga
    51361 aaaaaaatta aagattatat aaaagattat aactactaac aaagaggaac gcctctattt
    51421 cctgtggaaa tatatattaa ttgagtatat ttaagaaaat tttacttgtt tgatattata
    51481 tattatttat ttaatttatt tatagattat ataattag
//
LOCUS       DAOPCH010000002        28320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_1824_length_28320_cov_6.127932, whole genome shotgun sequence.
ACCESSION   DAOPCH010000002 DAOPCH010000000
VERSION     DAOPCH010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 28320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28320
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1824_length_28320_cov_6.127932"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PLU55_00305"
     CDS             <1..461
                     /locus_tag="PLU55_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86537.1"
                     /translation="KSSKQVSIKKRFKSSSCYMKVSVYPIKDYIIEIINTRGITLASH
                     EEKLDILFYTGTDLKELKELLNNSKAECVVLNNLKPKINSGFFWIYHEFNDWYSFLNP
                     DIIYTNTEVLAKELYRILGIQTKIKNLSFNKKKRMPNNFFEKVKEIMMWE"
     gene            480..14738
                     /locus_tag="PLU55_00310"
     CDS             480..14738
                     /locus_tag="PLU55_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPJ86538.1"
                     /translation="MKTHIVQPGEENLEIIAKKYNLNPGDLAIYNRIANNDNLVPGQE
                     IKLELPKDAKPIVVRLGKNDTLETVAKQFNTSVDRLMALNNLPNTNVSEGATLVAWDP
                     FRMNYTNISKAEDLDTLAKQLNTSKDLLLKSNPHVDPNDIKPNTSLFVPWQLNRYVEP
                     PKETKTYSPPKEAPKEEPVDEVPPKTEPLKVDKVPAYAGEKEGEFLLKNYYDHHVFLT
                     EKPTMKPSNWWDKLWGFNDLYREREKEKYIKDITDELLKATNDAFKAYKKAEKEGDRY
                     EMQKQLTWIDNTHKEFERITGIKKGFDPAVVVKDIMRPEARIAEEEANKMEKELALLR
                     AKETDIAGLPESVLSDDEALLDYSTDTFENLNEVQTEGIKKGYIVRKDIDPKTVNPES
                     IDAIINYSGFSLTDKAKSMLDDFKKANDIPYDISKLSENLTKYFGEFNTDISNALYEQ
                     DYNKRVKNQKEFLRKYEDLAIVHEQINPNFLKYNQLYLDYSDLNNKIENILLDLKSIN
                     NSDIILEALTSGNLEAYKERLDSLGIKQSDIAKVGNLLSEYEAYSDKIVAIQSGFSQI
                     NQDLENDLELFTKALNNFDTNLNIKEFFISEANPTSEFGKSIKSLATLSFEASKLRGS
                     TIEDNKRLVEFQDFLNWYQSYVEAATSNFNAISLLNIRNDMWQNISGLTEQRKSEAKN
                     FANWFNPKYNDITFGERAYTTFVESLVNLTKVPVVGDVAEKVFSALDWYNNVALVEYV
                     FPVAKTYRSTVDMAGWLLPRKLELEANLLVGNYSEEEKERQRREFEEKFQQQAETRFG
                     LKDYDLRNIDLVWSHFATGSLEKKVIPIYDSNGNMMKDSNGNIMTSEIFYNKIRLQRD
                     WDLFEVLKNGNRLDLLQDIRDLNEGKDLRNLKAGDYINLPKLDYGSRGPLIRGGEDFW
                     WSPQDYFENAITNPTATEGVNLLVEIFLDPGTYILKSPYALAKGSIVSPIKFLTGKNI
                     KGLSAITNEALIAGHKALEHNFSFPTFRKFFPKIQDLHASWNKKVIEGIEAFAPKEKK
                     ATSWWQIPAQIDAIQGKTLNIVKSYPVSRLAIEGISNTIDLFSKPVDQFSFKLLTEAN
                     SSVLNIFDNFKLDYLSDHVPTTKTVFNKIKEHQVVSVNLSSGKKIVGVVNRAPSGAGL
                     RGSIRTKGKDIILDSNSFDVKLKDGTVVTVYEKNVSDIFTFNSKEVREMTTSWKKTNP
                     KEKYFYDTIKYNLPGIKVNKVNTFNLKKGDYIVSGQDIGSVVSVKDNKVKIKTFDNNE
                     NLIEKEIIPTKEVSLIDEEYIKNLNFNWKNAYANTLLPLKKALAVTTGGIQWFIDYLA
                     LGTLLNRLPFELRARANNLINKATVTLIAKSSLSATEIWKYFADAFEDSQRRYLLTRK
                     LRIDNKWVELKWNEVWNSKTKKIEVVENPDKKFVLEEKFRSLLLEPGGASLDDWKILS
                     KYNVYPSDIYKAIQKLLIRLSRGDEVANLLKQKSDLINDLIKVLDVNNLNLEGRRISS
                     HFNEDWNKYLIKKGITSRKVREDIGHAANDFGKKIANIDDENLISTARNEIKQIYGEF
                     VSKEAEAVWNIYKEYVSDLLEKQKTQIDSVIVDLPAGVDITFESVEKKLGRLDEIVFQ
                     KRTFVNRGGEPVYVFEPGAKKADMIEAIGRLSYEEKLLQVNEKILEGFNVAKIIEDFY
                     EDAFIGNKKTYKLYKDYLAVNKKAELPDFIAWVDTYQSLKRSDFSSYKLLKDTISKQI
                     KGSSPSDKLRLELMELLDSKFPLLRTHDYTKTVDTLLNAKRMSDKAFSDAAKVLDHYL
                     NKRRHPVVLSNKKFMVIDTETTGLNVAKDDAFQFSWKIIDGDGKVIDTHNYYRAFDSK
                     FTEAKFLSEDALKAQKVHGITIEKLKESNPKDIKEIINAFAESISSVDSIIAHNSDFD
                     IEFIKKAIEEADGSPDAIFRGIDIIDTNKRNVIDKGWEGGKLDDLYRKYMGGSLVGAH
                     NALKDVDALIDIYKKALREGKLNERIFKSQEKNIYNNLVKLRDQLDVFQKRIELPVGI
                     GKQFNFVATKTLTPERIKVIREEVARNLFNQESERLFNLSTEDIVKELSIPGAISKAI
                     KDGQVAHSPRKNIIGDFKDKNIRDILFFNQDKFKEIDSKIKKINKDLEKGKLTSVEKK
                     KLIKELDTLNFEKSQYDPIKNNLPENSGIRVISEDYVPDEKELADLKKLIDDGWRFVV
                     YSKSRSRLSNDPPAVKLLRSLGLAEEYQMKSPEAAGKLFDFYVYNTKSNKLLSRDLVG
                     ITSEYVDEYTTVPGILGILYRSTDSKEYIVSLYKKLSKKLKTVDPEEDITKVINSSYF
                     FDTIYNFVIRNKNIFKGKEFEEFLPRFRFLKNLRKAKSSPDVSILMINSIENLKLNNV
                     NVDVVLLGKLNKEQLKAVNHFNDVIEMEELNLSPNIRLEDKINKTREIKKYNDEISIK
                     LEAEDKVAYPKIELTNEDLNAVKKIIDDVSSKVFDDLTPLKKFVKNQDIDIEKSLIDG
                     ENISIESYVNKLFKETEEDYRALRFSEFEITKKGSEEEPLAGSSGDLIKHTFDRWIDD
                     QLDILNIVSYEDLDTIKSLMKSIGAKEQIRENFQGKKVSVYEISLNDAYYYRVKGLDP
                     NALIHKPKVTKKEYLNVKTSYIVPGVYGGKTVNFVFGDGSFIIKGHYGHFKNVDNKIV
                     YSIDTSPENIKKLDLKDGDIIYTNTTMAENFKKFNATDAIFPVKVKEYNRDYLDEPSN
                     IVDLTRGSKDNVYDLYIIKDGKLKEIKSKIDKDNLTELIEKNIDTEKKSYYLLDDVGW
                     RNDKYSDVSFIVEEMPSLDLPNQHSEVIVPKRATITETIEVKEEFPDSFAIKKEDGTS
                     MFIEGNTKTKINNIIFKSKSTGDYSLFFPPKFLFDFDKIDLDRTRLIKKLLPEDSPKT
                     DYIVIDVPNNSYINKLKKDLDTLDLDALKKYDLEKDIEKAIKERDSADAFEKVIKETD
                     ELKGIKIYRISSIEGIALEENLESINKFRDSRFNKENGLFISSNPDKTKDFLDFKLFT
                     PRESKILENMNNVFTEIKDLVRSEKLHGKSAFYTKQFSIFSEPVNLDKSIESLVRNIE
                     RGAAVDDLEKVILETVTKTKRAWDGKNVVVIALDDATSLTGEFYEAINDIIEKNKRLK
                     TKTKILFPDHPNLLERDAKAYLLSRGFDENLIGVVDLDHVPVAKTYKDFKITDQTKAI
                     KDLINLQNEKMSRASYSLVIGKIEDDVAGIIPKRLLLAQNSKEAGILVMSDNLILKDQ
                     INVMKGFLVENTDSAYKFPSAGIRDELFNKKITFQRALEKTIIASSRKEFQKEWVKFI
                     NEETNAYRETVESQYKPYLAGGSNIELELYREMVEEQVQRFRDAFKPIEKYILKWDGS
                     EPVLDKYLRSARERVLKDLINDIAEGKRDRAIFENYLEKGDVPTVGYFLRKNLGYNTT
                     KFLIALPGYLQTIFIQSVLRLMPRWSINNALDDLIKTLIFDRDFKKFLSGIWLQGKIF
                     VNFYGTLLKHGKIDVSNSIMSNFFRLQKKLGIINNKEFNLKIRKLPKKGFYDLSELLK
                     EWGHEDWSKMIRYIDDITIVKKDKKGKSILDKEGREVSQTYSAIDLDYMMSTGIQEIY
                     SSPEEALLYFKRIIPKNANPLERIGFKAKEWNANLDMFYSQTERLRRAIALQRALQDQ
                     TIDMAKAKAFVSDFFFDYKAVNRAGVFFRKFFPFFTFSYFTARLYLKLLLGRYGYQTF
                     RAGVAIMQIWEANTAHLPEMYKDRISIKIGDREFLIRPNISLIDVMRFVFDPSKEIEQ
                     TFENPRKLFFGLGYGPIVGPIVEELTGATYFKPSESEMKAMGWNYGEIKKELQKYEDS
                     KAGETWIDTWWKLVYSIVPHIEIIEKLFFKADVEFQQTGNILKSSKFREISKMFAINI
                     LERDAVDAFTQKLYSLPPSVRNYYINQIKESDPELYEEWQRQSLISLNNNLIVKGVSE
                     EERIRTLDKRRAQNEYYRLEAQEKGAGSEWLDKNPYQKELLEEVWRDSPKSAYDEYRE
                     KLRLIDEDRRTIRQIVEETLPSNLENEKNIERYKALGYDYQPVNRDKLIKDLTNKDLN
                     IHQMNVLLAYKYPDVGLDFIRKSQIDQKRAESEEYKALSLEEKKELSLADQAYYKKIN
                     ILNRFLPDKDATEEEVQKAFELRKKQIDKIFTPEEKKRYLDSIPESQKILEKAMTDYI
                     ERWGKIIENSEDGKYWESLNSQPSWFKEFYFLNNPEVRVYLPLALEREKRLGEVIEME
                     KRGFSTIKERKALNDWFWAHEKEIKAWESHSPETIERYRERKEYDENFYFPLQSFANI
                     KNWQGYYNFLLSPSNKKYLDMWSRMGSSERTEEQIKEEAEQKIKMAKLSKEYYSLPST
                     TWNDQKKKQEWLNNNSELRDWWDRNKSKEELELSKKAQVYFDMKFDIEDEGSGYDYYL
                     KFREINVKRQDYLNNNPDVLKYINSNEEGTLLEPSTIKRKLAIYNTLSDEKKREYLDN
                     NKDLAKYFLDGVPSGIRKVRELQEEYFNIKESQFKTKDEFYDARNKFLKDNPNLKKYW
                     DAMAMPSSYFLDKQKFNKYQIDLSKVLKYYDSISANNLSDSMRISLLNMNSYMVPGNS
                     EEDKWMMNKVYESAMQTWIRLLDENKSVGMYFFRQLPTWIRARYYDNHPDKRVLSNYS
                     LNQWFGQPIWRYNKNNPSVNWALEQQRKYGKNIPISIKRQVEKILISAGEWEDRSSWT
                     SEKWKEWQTDRAARLNGLKQKDVDTNPLLRKELYRATVFFKRNAPIDPKIYNKKIKFM
                     NLPDNSVFLL"
     gene            14784..15413
                     /locus_tag="PLU55_00315"
     CDS             14784..15413
                     /locus_tag="PLU55_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86539.1"
                     /translation="MKIENFVKLCAESIKDTNFILIQQNEWLEILNSQINDLFPDIAI
                     IKNSLIDLSEYKRSPIQIDMGDGSKYPNIIGIKRIYIVNADGEKRLFDNWNFDKDIKL
                     LNLNVNPSSDYASFSDKILIVWIAEMEEKQKFSEEIELNNSQLQLLKKACSKEVISRI
                     LMDRIKLDRYKTLVGEANEYVLMAIQKNLSDEVEFSKRKRTNTLPVRSL"
     gene            15547..16008
                     /locus_tag="PLU55_00320"
     CDS             15547..16008
                     /locus_tag="PLU55_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86540.1"
                     /translation="MKEYSKLNKYKDYQKEYRKCDTYKSYQKRYSKSEKKKRYQNEYI
                     KTKYKNNLKYRLGCNMSSAVRSALKGKKGGRSWEDLVGYTVEDLMKCLESKFEPWMNW
                     ENHGEWQIDHIKPISLFNFTNIEDNEFKECWALSNLQPLGKIENRRKSNKY"
     gene            16067..18652
                     /locus_tag="PLU55_00325"
     CDS             16067..18652
                     /locus_tag="PLU55_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86541.1"
                     /translation="MIEPKAARVLPAPLMGNKFSTGSVNYSDLDFWQSAAMTNFTRGF
                     NQKFFSDVNSYWFSTGIDVSTPGEFCLERDIVTDVPLTSDPLLLNIYGKVTASYKTLT
                     HLYVGTSTGYVFSRAVDSSTWVLISAITSDSNPVTGFFEVSDQYSDSDKYLYVCKGEY
                     KGWILIDSEWMQLAPIIEWAPSLSNLEGVKLGNSGTLTKRKIAQSFILRSNERAKRFS
                     IAYKTIGNWTGDVTVNIHLSDPITGAIAADSIIETFTVTLPKSSTMTWFNYDISDTAK
                     QFVLSRCDKFFIEIVPLTAANDTACPEFGCTIGRDSYYEGDTLIVLDPGEDNYAFAVK
                     GEQLAFKIWSDLPTGVSLVSSQGFSAFGWVDNGIKSTANGVLWSPDSSKGLWTLPASQ
                     GKAKALGRTGRGTIVGGASSLWLFVGGSSAMNLWDFPDYINDNNFRGFHEWNNKIIFS
                     IENQGIFMTDGSTVGQSNLNNEREAFKFISCNGITTSGWDAFALCKNDKSEWYLLRSN
                     AYYDGSFIYWWAVKKLSKEPTHIASLNVNTILIFNEDKSIDKYCRNGTYQSTGYLETS
                     LFDAGLIKLDKLYKDIEVMFEPFETDTSCSIGYRTSIEDNYTTSEVFTSSGKHSVLYP
                     LYNPTSGNRFQVKVTLNSNTNKTKTPICSDIVWSYILQRSANEKNTLKTFYVTIICEP
                     SLENNIGNVEFINAEGSRSSKDILNDLWELSQKREVLNYIGMDNESNVAFKLEYNGEK
                     KYTIKIDRTNYLFSILNGTTVKYTFNYRDKTTNDIVDDLNERVLTGDTEMILCSLVDP
                     FKGTESVNMLEPIKDVYLTNETYLNIGTDVRAVLLNPQSPSQVKISLDKYGRDRIQLN
                     LREAN"
     gene            18743..19477
                     /locus_tag="PLU55_00330"
     CDS             18743..19477
                     /locus_tag="PLU55_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86542.1"
                     /translation="MSRKKTYNERLAEGVLAGRGYEVLDSTNKRKNIHENIAYDPTLY
                     IPGTLPEKIVLNHLISLQVNFNFQYHFMNNYATSYPEDVWVADFYLEDYNTYIEIYGT
                     YWHSTSDSAENDLIKKVYWLNSGYEILEKGLSMHPSGMATIGKVCIWWEEEIYRGVAQ
                     LFNRDLPEVMSNRIPGKMKELTFDVYAEWNAKEKREASINLKKIIPKRVTPKEDFKKL
                     RDRTLVQNIYGKSKNPRRIYRGKGLS"
     gene            19425..>21042
                     /locus_tag="PLU55_00335"
     CDS             19425..>21042
                     /locus_tag="PLU55_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPJ86543.1"
                     /translation="MENQKTPEEYTGVKGLVRSIYQETATSFVDSNNNYVTVFPDKIE
                     YIKTQDICTGGAKTSTGSVSATGSGNINPSAISSGRIASYLTVGDLVAIDGTRGLISL
                     GIPSSGVVGDNSYMYMNASGIFGYHGTDTFPWFFAATKDMVFGSRTYHGGDFFIGEID
                     SAYLWWQYDEHKLIIAGDISISGDIESSNFEYDPFTGTLVGYKLEYYGGNAYFGGAIV
                     SGNSFITGNTSIYGNNVTIAGQSIVNVGNVSDSTALTIPAGLLVDGTAITIANDGTIS
                     ANITLSWTAETDPRFDHYQIRYKKSSFTYYQYISSNSNTITIDGLTPNTSYDFAVSSV
                     NKYGIPSTYSSTVTYTTATSTTAPAKVSGVSAVGGIQYVIVEWISNSEKDLSSYNVYR
                     NTVNNSETATLIGNCKTNYFVDGGRTGGTEYFYWVKAVNTSGLVSTDFSTVASATPRN
                     VTNDDVVNLASSKVLIDGVTYLSNWRKSGDLTKIDGGSISTGSITTTQLNFTPVIDGT
                     IIASINASEEGIKISGEHIAISGSTTFSSGY"
     assembly_gap    21043..21142
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21143..22459
                     /locus_tag="PLU55_00340"
     CDS             <21143..22459
                     /locus_tag="PLU55_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86544.1"
                     /translation="ISGEHIAISGSTTFSSGYDPITKVNKLGGSYASASSGARVLIFP
                     DTNTGLQIIDNGGADVFKAIIGGTNVGDVIIGNYTGGQGIFYDKSEAKTIFKGSMIAG
                     NITGVTITGGTIQTAISGKRVEIKTEGKYENSIAIFDSTGNIGIHLFDNSILGGWTNS
                     AIIFDQSYGLRVAFPFPTSDQGYIYFLRNSSQSSGTAAPIIKGSPSLAIEVNSITLSG
                     TTRTSWPSSFSGNLSDLYINTTKSWGSYGITGLGTITPYSNNSYSLGSSSYKWNNVYS
                     TYGTFDYLGKSLNANSYGITGLGTITPYSNGSYNLGSSSYYWNYVYGINIIPSGKYTS
                     YLGNSSYHWDYFYVDTIRGAGGTDSIEIENNAFTRCLFPADNVKYDIGGSGFFYKNVF
                     TQSLRLRTGQSTNPANDGEIRYYDVEGGGYRGRVRGYLMQFDGTLK"
     gene            22465..22815
                     /locus_tag="PLU55_00345"
     CDS             22465..22815
                     /locus_tag="PLU55_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86545.1"
                     /translation="MEKYTDKNLSKEERISEYNKEIDKLMNPIPEEEFDPQTFDPIKI
                     EGMEDLVVRKRAIKKGKLPTYGLLPKEIREGQMIGMYESKQDIYLLLANKINELYDEI
                     KSLSDEIKKIKDNK"
     gene            22819..23181
                     /locus_tag="PLU55_00350"
     CDS             22819..23181
                     /locus_tag="PLU55_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86546.1"
                     /translation="MANEYQGTSIVDYLKSIGQNSSYQARASLASQYGRSRGTGIIGQ
                     DGSCIITPESHFILVTEPEGLVTVNLTATEEDQGLYVYHIEKNEYIEVRGKSGSSFMY
                     EILAIRKGYLNSKVEIDN"
     gene            23193..23486
                     /locus_tag="PLU55_00355"
     CDS             23193..23486
                     /locus_tag="PLU55_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86547.1"
                     /translation="MKINESLKTISGNYFTLDDGKTKLTFKDAAIICCETYKSEKSIP
                     GQALKIIAIGSKIFNAKDEVEFTEEEKALLREVIEGNKVYLTGVTGAMLKLLS"
     gene            23624..24259
                     /locus_tag="PLU55_00360"
     CDS             23624..24259
                     /locus_tag="PLU55_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86548.1"
                     /translation="MIPNKVTGAGNIDATMAYKVKFSKALSSTPKIEAWDNSETFPAV
                     GTTSTTTKEIFTGTAGNGNKPMLAGYIGGLVGAATKPGSNWHPASATAGAANPNLLKG
                     NTNYVMAAVVPGENDELVFNLSLRVPYDATVPSTNSMNALIRIVYNYTGMAPDITLAF
                     NEGTEAVPSWTSFIAGTHGIRFCNAEVVGTDYKLTLPEEGQVYAGSQYVTI"
     gene            24256..26997
                     /locus_tag="PLU55_00365"
     CDS             24256..26997
                     /locus_tag="PLU55_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86549.1"
                     /translation="MRIRCTNCGIYFFRDSQDEVYTLCDKCLKELIKEGSAPVVPVSA
                     SATLSFTGDINQGDTITINGRTYEFDIDGSIRPGNIPIDVTGKAVAATGTLTATQPVV
                     LGKKVTIGTDDYTIDDESGDFKINVGAGTKVAATGTLSLTGAITPGSHAETVLTSTGV
                     APTPITQATKIFNFTGVASDGETITLGARTYEFDNDSSILPSNVLVDISAGTKFQATT
                     KFSLALPILDGETVSIGDEVYEFDIDGLVTSGNIAVDVSSFSTISQGTITISGTPIDQ
                     EKMTIGAVEYTFVAARSGAGEITINANNDIQRDNIISAINADSTDVTAQSGGSGLVTV
                     LAAEDGAAGNLITFTENVTGIAMNGDGTLGGTQKAIDCDAIHAVATLLSTFNANTAYD
                     ITGVPYGTNAIDFTADAAGALDGSLGNISASTTTDGTISNFSGGADATGIEAATALVA
                     TVAADIIAAPEIVNVTAEAGGDGEFTAKALTAGTVGNAIVIGETTANGTFAGGATLLS
                     GGLEADTVTIGSIEYTAVASLSAPTVANEVMIGASAAEFLDNLKSAVNQSAGSGSAYS
                     VGTVKNPIVVATDNTDTTQKFIAREPGTAANTADTTTTAVTLSFEDTTLGGGTGSSVP
                     GVNAETVVIGGKTYSFVDVLSETNGATAIVNQVLFGSDSSVALDNLKLAVNAEGTAGS
                     SYSTGTIKNADVLATTNESNAQTFEALVSGSAGNAITTTTTIVNGSFGGATLSGGSDA
                     TAAEFIAAIVTTINAITGNIVTAENETGGVMKVTAAVAGAAGNSIVTISNVGGATFGA
                     ATLTGGKDAGSTDACAETKATINADTVSVVSAETIDASSIKIVAKTPGDIGNIIKVST
                     DGDNIAFDKASLSGGVNGTPGWTNQILSDGSKLYISSELSTSTDSNWKEVDINEVEE"
     gene            27012..27221
                     /locus_tag="PLU55_00370"
     CDS             27012..27221
                     /locus_tag="PLU55_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86550.1"
                     /translation="MRIQCLRCGLMITVDQQDIAMHPRLCDKCLTVAVQSSGEEIEDE
                     EVKEEITPEELGIEEEVVKNKGRNR"
     gene            27374..27787
                     /locus_tag="PLU55_00375"
     CDS             27374..27787
                     /locus_tag="PLU55_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86551.1"
                     /translation="MFIVQTKDGKTFVEGKEGFVWDNIPDDVEITSLSLTLPFKVSFK
                     TKSGDILLNPKFTIKDFDSYFFSNEETISILAVNSILGKSNRVLTAKIIGGIKGDNVF
                     EYRMDRHGNIKSRIFSFSELEKSYNLSAIRKGLTN"
     gene            27919..>28320
                     /locus_tag="PLU55_00380"
     CDS             27919..>28320
                     /locus_tag="PLU55_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86552.1"
                     /translation="MLDAAYNFAKTTISPFGSGATSATLTDPTRFLDPSTKQYNCVVW
                     CSSLYPNPIDDPNKEIVRFTAKSGSVYTFTRAQEGTEAKDHNIEDEIYSVAIVLTEKM
                     ISDIQDELDDKEPVLTKGNLSGSGVITIDNTR"
BASE COUNT        10809 a         3102 c         5164 g         9145 t
ORIGIN      
        1 agaagtcgag caagcaggtt agcattaaga agaggttcaa atcctcttct tgctatatga
       61 aagtatcagt ttatcctatt aaggattata taattgaaat tataaataca agagggatta
      121 ctcttgcaag ccatgaagaa aagttagaca ttttattcta tactggtaca gacttgaaag
      181 agttaaaaga gcttcttaat aattcaaaag ctgaatgtgt tgttttgaat aatttaaaac
      241 caaaaattaa ttctggtttc ttttggatat atcatgagtt taatgattgg tattcttttc
      301 ttaatcctga tataatatat actaacacag aagtattagc taaagagtta tataggatat
      361 taggtataca aacaaaaata aagaatcttt cttttaataa gaaaaaaaga atgcctaata
      421 atttctttga gaaagtaaaa gaaattatga tgtgggaata attgtaataa aataaaataa
      481 tgaagacgca tattgttcaa ccaggggagg aaaatttaga gataattgct aaaaagtata
      541 atttaaatcc aggagattta gctatttata atagaatagc taataatgac aatcttgtac
      601 ctggacaaga aattaagtta gagcttccta aagatgctaa acctatcgtt gttagacttg
      661 gaaagaatga tactcttgag actgttgcta aacagtttaa tacaagtgta gataggttaa
      721 tggctttgaa caatttgcct aatacgaatg tatcagaggg tgccacgctt gttgcttggg
      781 atccttttcg tatgaattat actaatataa gtaaagcaga agatctagat actcttgcta
      841 aacagcttaa tacaagtaag gatctcttat taaagagtaa tcctcatgtt gatcctaatg
      901 atattaagcc aaatacttct ttatttgttc catggcagtt aaatagatat gtagagccac
      961 caaaagaaac gaaaacatat tcgcctccta aagaagctcc taaggaagaa ccagtagatg
     1021 aggttcctcc taaaactgaa ccattaaaag tagataaggt tcctgcttat gctggagaaa
     1081 aagagggaga attcttatta aagaattatt atgatcatca tgtcttttta actgagaaac
     1141 ctacaatgaa accaagtaat tggtgggata aactttgggg atttaatgat ctttatagag
     1201 aaagagaaaa ggagaagtat ataaaagata ttactgatga gcttttaaag gcaactaatg
     1261 atgcttttaa agcttataag aaagctgaaa aagaaggaga tagatatgag atgcaaaagc
     1321 aacttacatg gatagataat actcataaag agtttgaaag aattactggt attaaaaagg
     1381 gctttgatcc tgctgtagtt gttaaagata taatgagacc agaagcaaga attgctgaag
     1441 aagaagctaa taaaatggaa aaggaattgg ctcttcttag agcaaaagaa actgatatag
     1501 ctggattacc agagtctgtt ttatcagatg atgaagcatt attagattat tctactgata
     1561 cttttgaaaa tttaaatgag gttcagactg aaggaattaa aaagggatat atagttagaa
     1621 aagatattga tccaaaaact gttaatcctg aaagtattga tgctattatt aattattctg
     1681 gtttttcttt gacagataag gcaaagagta tgcttgatga ttttaaaaaa gctaatgata
     1741 ttccttatga tatatcaaag ttatcagaaa atttaactaa gtattttgga gaatttaata
     1801 ctgatattag taatgcttta tatgaacagg attataataa aagagtaaaa aatcaaaaag
     1861 aatttcttcg taagtatgaa gatttagcta ttgttcatga acaaattaat ccaaactttt
     1921 taaagtataa tcagctttat ttagattatt cagatttaaa taataagatt gaaaatattc
     1981 ttcttgattt aaagtcaata aataatagtg atattatttt agaggcgtta acttctggta
     2041 atcttgaggc atataaagaa agattagatt ctcttggaat taaacaatca gatattgcaa
     2101 aagttggtaa tttgttatca gagtatgaag catattctga taaaattgtt gctattcaat
     2161 caggtttttc ccagataaat caggatcttg aaaatgattt agagcttttt actaaggctt
     2221 taaataactt tgatactaat ttaaatatta aagagttttt tatttctgaa gctaatccaa
     2281 ctagtgagtt tggaaagtca attaagtctt tagcaactct tagttttgaa gcatctaaac
     2341 ttagaggttc tactatagaa gataataaga gacttgtaga gtttcaggac tttttaaatt
     2401 ggtatcaaag ttatgtagag gcagctacat ctaattttaa tgctatttct ttgttaaata
     2461 tcagaaatga tatgtggcaa aatataagtg ggttaacaga acaaagaaaa tctgaagcaa
     2521 agaatttcgc taattggttt aatcctaaat ataacgatat aacttttgga gaaagagctt
     2581 atactacttt tgtagaatca cttgttaatc ttactaaagt tccagtagtt ggtgatgttg
     2641 cagaaaaggt tttctctgca ttagattggt ataataatgt tgcccttgtt gaatatgttt
     2701 ttcctgtggc taaaacttat agatcaacag ttgatatggc tggttggtta ttgccacgta
     2761 aacttgaatt agaagctaat ttattagttg gaaattattc agaagaggaa aaagaaagac
     2821 agagaagaga gtttgaagaa aagtttcaac aacaagcaga aacaagattt ggtttaaaag
     2881 attatgattt aagaaatatt gatttagttt ggagtcattt tgcaactggt agtttagaga
     2941 aaaaagttat accaatctat gattctaatg ggaatatgat gaaggatagt aatggaaata
     3001 ttatgacttc tgaaatattc tataataaaa taaggcttca aagagattgg gatttatttg
     3061 aagttcttaa aaatggtaat agattagatt tacttcaaga tattagagat cttaatgaag
     3121 gaaaagattt acgtaattta aaagctggag attatataaa tcttccaaaa ttagattatg
     3181 gatctcgtgg acccttaata agagggggtg aggatttttg gtggagtcct caagattatt
     3241 ttgagaatgc aataacaaat ccaacagcta ctgaaggagt aaatctatta gtagaaatat
     3301 ttttagatcc aggaacttat attcttaaga gtccatatgc tttagctaaa ggctctatag
     3361 tttctcctat taagttttta actggaaaga atattaaagg attatcagct attactaatg
     3421 aagcattaat agctggacat aaagctcttg aacataattt tagttttcca acatttagaa
     3481 agttctttcc taagattcaa gatttacatg cttcttggaa taaaaaagtt attgaaggaa
     3541 tagaagcttt tgctcctaaa gaaaaaaagg caacaagttg gtggcaaatt ccagctcaaa
     3601 tagatgctat tcaaggtaag acactaaata ttgtaaagag ttatccagtt agtcgtcttg
     3661 ctattgaagg aatttctaat actattgatt tgttttcaaa gccagttgat caattttctt
     3721 ttaagctttt aacagaagct aatagttctg ttcttaatat ttttgataat tttaaattag
     3781 attatttatc tgatcatgtt cctacaacta aaacagtttt taataaaatt aaagagcatc
     3841 aagttgtatc tgttaatctt tcttctggaa agaaaattgt tggagttgtt aatagagctc
     3901 catctggagc tggattaaga ggaagtataa gaactaaagg aaaagatatt atcttagatt
     3961 ctaatagttt tgatgttaaa cttaaggatg gaacagttgt aactgtatat gaaaaaaatg
     4021 tttcagatat ttttactttt aattcaaaag aagtaagaga gatgactact tcttggaaga
     4081 agactaatcc taaagagaaa tatttttatg atactataaa gtataatctt cctggaataa
     4141 aagtaaataa ggttaatact tttaatctta aaaaaggaga ttatatagtt tcaggtcaag
     4201 atattggatc agttgttagt gtaaaagata ataaagttaa gattaaaact tttgataata
     4261 atgaaaatct aatagaaaaa gagattattc ctacaaaaga agtatctctt attgatgaag
     4321 agtatattaa aaatcttaac tttaattgga aaaatgctta tgctaataca ttacttccac
     4381 ttaaaaaggc tcttgctgta acaactggag gtattcaatg gttcattgat tatcttgctc
     4441 ttggaacttt attaaataga cttccatttg aattaagagc tagggctaat aatttaatta
     4501 ataaagcaac agttacatta attgcaaaat ctagtttaag tgcaactgaa atttggaaat
     4561 attttgctga tgcttttgaa gatagtcaga gaagatattt attaactaga aaacttagaa
     4621 ttgataataa gtgggttgag ttaaaatgga atgaagtttg gaattctaaa acaaaaaaga
     4681 tagaagtagt tgaaaatcca gataagaagt ttgttcttga ggaaaagttt agaagtttat
     4741 tattagaacc aggaggagca tctttagatg attggaaaat cctttctaaa tataatgtat
     4801 atccttctga tatttataaa gctatacaga agttattaat aagactttca agaggagatg
     4861 aagttgctaa tttattaaaa caaaaaagtg atttaattaa tgatcttatt aaagttcttg
     4921 atgtaaataa tttaaatctt gaaggaagac gaattagctc tcattttaat gaagattgga
     4981 ataaatactt aataaaaaag ggaataacaa gtagaaaagt aagagaggat attggacatg
     5041 ctgctaatga ttttggaaaa aagatagcta atattgatga tgaaaattta atatctactg
     5101 ctagaaatga aattaaacaa atttatggag aattcgtttc taaagaagca gaagctgttt
     5161 ggaatattta taaagaatat gttagtgatt tattagagaa acaaaaaaca caaatagatt
     5221 ctgttattgt agatcttcct gctggagttg atattacatt tgaatcagtt gagaaaaagt
     5281 taggaagatt agatgaaatt gtttttcaaa aaagaacctt tgtaaataga ggaggagaac
     5341 cagtatatgt ttttgagcct ggagctaaga aagctgatat gattgaagct attggaaggt
     5401 tatcttatga agaaaaacta cttcaagtta atgagaaaat attagaaggc tttaatgttg
     5461 caaagattat tgaagatttt tatgaagatg cttttattgg aaataaaaag acctataagt
     5521 tatataaaga ttatttagct gtaaataaaa aggctgaatt accagatttt atagcttggg
     5581 ttgatactta tcagtcttta aagaggagtg attttagtag ttataaatta ttaaaagata
     5641 ctattagtaa gcaaataaaa ggatcatctc cttctgataa attgagactt gagttaatgg
     5701 aattattaga ttcaaagttt ccattacttc gtactcatga ttatactaaa actgtagata
     5761 ctcttttaaa tgcaaagaga atgtcagata aagctttcag tgatgctgct aaagttttag
     5821 atcattatct aaataaaagg agacatccag tagttttaag taataagaag tttatggtta
     5881 tagatactga aactactgga cttaatgttg ctaaagatga tgcttttcaa ttttcttgga
     5941 agataattga tggtgatgga aaagttatag atactcataa ttattataga gcttttgata
     6001 gtaagtttac tgaagctaaa tttttaagtg aagatgcttt aaaagcacaa aaagttcatg
     6061 gaattactat tgagaaatta aaagaaagta atccaaaaga tattaaagaa attataaatg
     6121 cttttgctga aagtattagt tctgtagatt ctattattgc tcataattct gattttgata
     6181 ttgaatttat taagaaggca atagaagaag ctgatggatc tcctgatgct atttttagag
     6241 gtatagatat tattgatact aataaaagaa atgttataga taaaggatgg gaaggaggaa
     6301 agttagatga tttgtataga aagtatatgg gaggaagttt agttggtgcc cataatgctt
     6361 taaaagatgt agatgcttta attgatatat ataagaaagc tcttagagaa ggaaaactta
     6421 atgagcgaat ctttaaatct caagagaaaa atatttataa taatttagtc aagttaagag
     6481 atcaattaga tgtttttcaa aaaagaatag agcttcctgt tggaatagga aagcaattta
     6541 attttgtagc aactaaaact cttactcctg aaagaataaa agtaattaga gaagaggttg
     6601 ctagaaattt atttaatcaa gaatctgaaa gattatttaa tttatctaca gaagatattg
     6661 ttaaggagct aagtattcct ggagcaatat ctaaagctat aaaagatgga caagtagctc
     6721 attctcctcg taaaaatata attggagatt ttaaagataa aaatattaga gatattcttt
     6781 tctttaatca agataagttt aaagaaatag attcaaaaat taaaaagatt aataaagatc
     6841 ttgagaaagg taaacttact tctgtagaaa agaagaaact tattaaagag cttgatactc
     6901 ttaactttga aaaatctcaa tatgatccta taaagaataa tcttcctgag aattctggaa
     6961 taagagttat tagtgaagat tatgttccag atgaaaaaga gttagctgat ttaaagaagc
     7021 taatagatga tggttggaga tttgtagttt attctaagag tagaagtcgt ttaagtaatg
     7081 atcctcctgc tgttaaattg cttagatctt taggattggc agaagaatat caaatgaaaa
     7141 gtccagaagc agctgggaaa ctttttgatt tttatgttta taatactaaa tcaaataaat
     7201 tattatctag agatttagtt ggaattacat ctgagtatgt agatgaatat acaactgttc
     7261 ctggaatttt gggtattctt tatagaagta ctgatagtaa ggaatatatt gtcagtttat
     7321 ataaaaaatt atctaaaaag cttaaaacag ttgatcctga agaagatata actaaggtta
     7381 ttaattcttc ttatttcttt gatacaattt ataactttgt tattaggaat aagaatatct
     7441 ttaaagggaa agagtttgaa gaatttcttc caagatttag atttctaaag aatttaagaa
     7501 aagcaaaaag ctctcctgat gtttctattc ttatgattaa ttctatagag aatttaaagc
     7561 ttaataatgt aaatgttgat gttgttcttt taggaaagct taataaagaa caattaaaag
     7621 ctgttaatca tttcaatgat gttattgaaa tggaagaatt aaatcttagt cctaatatta
     7681 gattagaaga taagattaat aaaacaagag agattaaaaa atataatgat gaaatttcaa
     7741 ttaaattaga agcagaagat aaagttgctt atcctaaaat agaattaaca aatgaagatc
     7801 ttaatgctgt taaaaagatt attgatgatg tttctagtaa agtatttgat gatttaactc
     7861 ctttgaaaaa gtttgtaaaa aatcaagata ttgatataga aaaatcttta atagatggag
     7921 aaaatattag tattgaaagt tatgtaaata aactttttaa agagactgaa gaagattata
     7981 gagctcttcg ttttagtgaa tttgaaatta ctaagaaggg atctgaagaa gaacctttag
     8041 caggttcctc tggagatctt ataaagcata cttttgatag atggatagat gatcaattag
     8101 atattttaaa tatagttagt tatgaggatc ttgatactat aaaaagttta atgaaaagta
     8161 ttggagcgaa agaacaaatt cgtgagaatt ttcaaggtaa aaaggtttct gtttatgaaa
     8221 taagtttaaa tgatgcttat tattatagag taaagggatt agatcctaat gcattaattc
     8281 ataaacctaa agtaacaaag aaagaatatt taaatgttaa aacttcttat attgttcctg
     8341 gtgtttatgg aggaaaaact gttaactttg tttttggaga tggtagtttt attattaaag
     8401 gacattatgg acattttaaa aatgttgata ataaaatagt ttattcaata gatactagtc
     8461 ctgaaaatat taagaagctt gacttaaaag atggagatat tatttataca aatactacaa
     8521 tggcagaaaa ctttaagaag tttaatgcta ctgatgctat atttcctgtt aaagtaaaag
     8581 aatataatag agattattta gatgaaccat ctaatattgt tgatttaact agaggttcta
     8641 aagataatgt ttatgatctt tatattatta aggatggaaa actaaaagaa ataaaaagta
     8701 aaatagataa agataattta actgaattaa ttgaaaaaaa tattgataca gaaaaaaagt
     8761 cttattattt acttgatgat gtaggatgga gaaatgataa atattctgat gttagtttta
     8821 ttgttgaaga gatgccatct cttgatttac ctaatcaaca tagtgaagtt atcgttccta
     8881 aaagagctac tattacagaa actattgaag ttaaagagga gtttccagat agttttgcta
     8941 ttaagaaaga agatggaact tctatgttta ttgaggggaa tacaaaaact aagataaata
     9001 atattatctt taagtctaaa tcaactggag attattcttt attctttcct ccaaaatttc
     9061 tctttgattt tgataaaatt gatcttgata gaactagatt aattaagaaa ttacttccag
     9121 aagattctcc taaaactgat tatattgtta tagatgttcc taataattca tatattaata
     9181 aattaaaaaa ggatttggat actcttgatc ttgatgcatt aaagaaatat gatcttgaaa
     9241 aagacattga gaaagcaata aaagaaagag atagtgcaga tgcttttgaa aaagttatca
     9301 aagaaacaga tgaattaaaa ggtattaaga tttatagaat tagttctatt gaaggaattg
     9361 cattagaaga gaatttagaa agtattaata agtttagaga tagccgcttt aataaagaga
     9421 atggattatt tatttcaagt aatccagata aaacaaaaga ttttttagat tttaagttat
     9481 ttactcctag ggaaagcaag atacttgaga atatgaataa tgtttttaca gagattaaag
     9541 atcttgttag aagtgaaaag cttcatggaa agagtgcatt ttatacaaaa caattttcta
     9601 ttttttcaga gcctgttaat ttggataaaa gtatagagtc tttggttaga aatatagaaa
     9661 gaggagctgc tgtagatgat cttgaaaaag ttatacttga aacagtaaca aaaactaaaa
     9721 gagcttggga tggtaaaaat gttgttgtta tagcattaga tgatgcaaca agtttaactg
     9781 gtgaattcta tgaagctatt aatgatatta ttgaaaagaa taaaagatta aaaactaaaa
     9841 ctaaaatatt atttccagat catcctaatc ttttagagcg agatgctaaa gcttacttat
     9901 taagtagagg ttttgatgaa aatctaattg gagtagttga tcttgatcat gttccagtag
     9961 caaaaaccta taaggatttt aagattacag atcaaacaaa agcaataaaa gatttaatta
    10021 atcttcagaa tgaaaagatg tccagagctt cttatagttt agttattggt aaaatagaag
    10081 atgatgttgc tggtattatt cctaaaagat tattattagc acaaaattct aaagaagctg
    10141 gaatcttagt tatgtctgat aatttaattt taaaggatca gatcaatgtt atgaaaggat
    10201 ttttggttga aaatactgat tcagcatata agtttccttc tgctggtatt agagatgagc
    10261 tctttaataa gaagataaca tttcaaagag ctttagaaaa aactataata gcatcttcaa
    10321 gaaaagaatt tcaaaaagaa tgggttaagt ttataaatga agaaacaaat gcatatagag
    10381 aaacagttga atctcaatat aaaccttatc ttgctggtgg atctaatata gaattagaat
    10441 tatacagaga aatggtagaa gagcaggttc aaagatttag agatgcattt aagccaatag
    10501 aaaaatacat attaaaatgg gatggaagtg agccagttct tgataagtat ttaagaagtg
    10561 caagagaaag agttttaaaa gatttaatta atgatattgc agaaggaaaa agagatagag
    10621 ctatttttga aaactattta gaaaaaggag atgttccaac agttggatat ttcttaagaa
    10681 agaatcttgg ttataataca actaaatttt taatagcatt gcctggctat ttacaaacaa
    10741 tatttattca atctgtttta agattgatgc ctcgttggag tattaataat gctcttgatg
    10801 atcttattaa gacacttatt tttgatagag attttaagaa atttctttct ggtatttggc
    10861 ttcagggaaa aatatttgtt aatttttatg gaacattatt aaaacatgga aagattgatg
    10921 ttagtaattc tataatgtct aatttcttta gacttcaaaa gaaacttgga ataattaata
    10981 ataaggaatt taatttaaaa ataagaaagc ttcctaagaa aggattttat gatttaagtg
    11041 aattattaaa agagtgggga catgaagatt ggagtaagat gataaggtat attgatgata
    11101 ttactattgt taaaaaagat aagaaaggta aatcaattct tgataaagaa ggaagagaag
    11161 ttagtcaaac atattctgct atagatctag attatatgat gtctactggt attcaagaaa
    11221 tttattcttc tccagaagaa gctttacttt attttaaaag aattattcct aagaatgcta
    11281 atcctttaga aagaattgga tttaaagcta aagaatggaa tgctaattta gatatgtttt
    11341 atagtcaaac tgaaagatta agaagggcta ttgctttaca aagagctctt caagatcaga
    11401 ctattgatat ggcaaaggct aaagcatttg tttctgattt cttctttgat tataaagctg
    11461 ttaatagggc aggtgttttc tttagaaagt tctttccttt ctttaccttc agttacttta
    11521 ctgcaagatt atatttgaag cttttattag gtagatatgg ttatcaaaca tttagagctg
    11581 gagttgctat aatgcaaata tgggaagcaa atactgctca tcttccagaa atgtataaag
    11641 atagaatctc tataaagata ggagatagag aatttcttat tagacctaat atatctttaa
    11701 ttgatgtaat gaggtttgtt tttgatccta gtaaggaaat agagcaaacc tttgaaaatc
    11761 ctagaaagtt attctttgga cttggatatg gtcctattgt tggtccaata gtagaagaac
    11821 ttactggagc tacatacttt aagccaagtg aatcagagat gaaagctatg ggttggaatt
    11881 atggagaaat aaaaaaagaa cttcagaagt atgaagattc aaaagctgga gaaacttgga
    11941 tagatacttg gtggaaactt gtttattcaa tagttcctca tattgaaatt attgaaaagt
    12001 tattttttaa agcagatgtt gagtttcaac aaactggaaa tatattaaaa agtagtaagt
    12061 ttagagagat ttctaaaatg tttgcaatta atatattaga aagagatgct gtagatgcct
    12121 ttactcaaaa gctatatagt cttcctccaa gtgttagaaa ttattatatt aatcaaatta
    12181 aagaaagtga tcctgaatta tatgaagagt ggcaaagaca atctcttata tctttaaata
    12241 ataatcttat agttaagggg gtttcagaag aagaaagaat aagaactcta gataaaagaa
    12301 gagcacaaaa tgaatattat agattagaag ctcaagaaaa aggagctggt agtgaatggt
    12361 tagataaaaa tccttatcaa aaggagttat tagaagaagt ttggagagat tctcctaaaa
    12421 gtgcttatga tgaatataga gaaaagttaa gattaataga tgaagataga agaactatta
    12481 ggcagattgt tgaagaaact cttccaagta atcttgagaa tgagaaaaat attgaaagat
    12541 ataaagctct tggatatgat tatcaaccag ttaataggga taaattaatt aaagatttaa
    12601 caaataaaga tttaaatatc catcagatga atgtattgtt agcttataag tatcctgatg
    12661 taggattaga ttttataaga aaaagtcaaa tagatcaaaa aagagctgaa agtgaagaat
    12721 ataaagcttt atcattagaa gagaaaaaag aactttcatt agcagatcag gcatattata
    12781 agaagattaa tatattaaat agatttcttc cagataaaga tgcaactgaa gaagaggttc
    12841 aaaaagcttt tgaattaaga aagaagcaaa tagataagat ttttactcca gaagaaaaga
    12901 aaagatattt agattctatt cctgaatctc agaagatttt agagaaagca atgactgatt
    12961 atatagaaag atggggaaag attattgaga attctgaaga tggaaagtat tgggagtctt
    13021 taaattctca accatcatgg tttaaagagt tctattttct taataatcca gaggttaggg
    13081 tctatttacc attggctcta gagcgagaaa aacgcctcgg agaggtcata gaaatggaga
    13141 aacgaggatt tagtactatt aaggaaagaa aagccttaaa tgattggttt tgggctcatg
    13201 agaaggaaat aaaagcatgg gagagccata gtccagaaac aatagaaaga tatagagaga
    13261 gaaaagaata tgacgagaac ttctattttc cacttcaatc ttttgctaac attaaaaatt
    13321 ggcaaggata ttataatttc ttattaagtc cttctaataa gaaatattta gatatgtggt
    13381 ctagaatggg tagctcagaa agaacggaag aacaaattaa agaagaagca gaacaaaaga
    13441 ttaaaatggc taagttatct aaggaatatt attctcttcc ttctactact tggaatgatc
    13501 agaaaaagaa acaagaatgg ttaaataata attctgaatt aagagattgg tgggatagaa
    13561 ataaatcaaa agaagaatta gagctttcta aaaaggctca agtatacttt gatatgaagt
    13621 ttgatataga agatgaagga agtggttatg attattattt aaagtttaga gagattaatg
    13681 ttaaaagaca agattattta aataataatc cagatgtttt aaagtatata aattcaaatg
    13741 aagaaggaac tttattagag ccttcaacaa ttaaaagaaa attagctatt tataatactc
    13801 tttctgatga aaagaaaaga gagtatttag ataataataa ggacttagct aagtatttct
    13861 tagatggagt tccttctgga ataagaaaag ttagagaact tcaagaagaa tattttaata
    13921 ttaaagaatc tcaatttaaa acaaaggatg agttttatga tgcaaggaat aaattcttaa
    13981 aagataatcc aaatcttaaa aagtattggg atgctatggc tatgccttct tcttatttct
    14041 tagataaaca aaagtttaat aaatatcaga tagatcttag taaggtatta aagtattatg
    14101 attctatttc tgctaataac ttaagtgatt ctatgagaat ttctttatta aatatgaatt
    14161 cttatatggt tccaggaaat tctgaagaag ataaatggat gatgaataag gtttatgaaa
    14221 gcgcaatgca aacatggatt agattattag atgaaaataa atctgttgga atgtatttct
    14281 ttagacaatt acctacttgg ataagagcaa gatattatga taatcatcca gataaaagag
    14341 tgttatctaa ttattctctt aatcaatggt ttggacaacc aatatggaga tataataaaa
    14401 ataatcctag tgttaattgg gctttagaac aacagagaaa gtatggaaag aatattccta
    14461 ttagcataaa gagacaagta gaaaagatat taattagtgc tggagagtgg gaagatagaa
    14521 gttcttggac tagtgagaaa tggaaagaat ggcaaacaga tagagctgca agattaaatg
    14581 gattaaagca aaaagatgta gatactaatc ctttattaag aaaagagtta tatagagcaa
    14641 cagtattctt taaaaggaat gctcctatag atcctaagat ttataataaa aagattaaat
    14701 ttatgaatct tccagacaat tcagtatttt tattatagtt ttttttgaca ctattgtatt
    14761 tactaggtat aattaaataa aatatgaaaa tagaaaactt tgtaaagttg tgcgcagaat
    14821 caattaaaga tacaaacttt atattaattc aacaaaatga atggttggag attttaaatt
    14881 ctcaaataaa tgatttattt ccagatatag ctataattaa aaattctcta attgatttat
    14941 ctgaatataa aagatctcca attcagatag atatgggaga tggatctaaa tatcctaata
    15001 ttattggaat taaaagaata tatatagtta atgctgatgg agagaaaagg ctttttgata
    15061 attggaattt tgataaagat attaaattat tgaatttaaa tgttaatcct tcttctgatt
    15121 atgcttcttt ttcagataaa atattaattg tatggatagc agaaatggaa gaaaagcaaa
    15181 agttttctga agaaatagaa ttaaataatt ctcaattaca attattaaaa aaggcttgtt
    15241 ctaaagaagt tatttctaga attttaatgg acaggattaa gctagataga tataaaacat
    15301 tagtaggaga agctaatgag tatgttctta tggctattca aaagaattta tcagatgaag
    15361 tagagttttc taaaagaaaa agaactaata ccttacctgt tagaagttta taaataaacg
    15421 aaaacgtttt cgcttatgaa gatatgtaca cattgcaaaa aacagaaaag taaattatcc
    15481 tttcataaag atattagata taaagatgga tttaatccat ggtgcattga ttgtcggaag
    15541 gaatacatga aagaatatag taaacttaat aaatataaag attatcaaaa agaatatagg
    15601 aaatgtgata cctataaatc ttatcaaaaa agatattcta agagtgaaaa aaagaaaaga
    15661 tatcaaaatg aatatataaa aacaaaatac aagaataatc taaaatatag gttaggttgt
    15721 aatatgtctt cggcagttcg ttctgcatta aaaggtaaaa aaggtggtag atcatgggaa
    15781 gatttagttg gatatacagt agaagattta atgaaatgtt tggaaagtaa gtttgagcca
    15841 tggatgaatt gggaaaatca tggagaatgg cagattgatc atattaaacc tatttcatta
    15901 tttaatttta cgaatataga agataatgaa tttaaagagt gttgggcgtt aagtaattta
    15961 caaccattag gaaagattga gaatagaaga aaaagcaata agtattaata ctaatagaat
    16021 gcaaatagaa aataaatatc atatttatct agactataag ggttatatga tagaaccaaa
    16081 agctgctaga gttttacctg caccattaat gggaaataaa tttagtactg gtagtgttaa
    16141 ctatagtgat ttagattttt ggcagtctgc tgctatgact aatttcacta gaggttttaa
    16201 tcagaaattt tttagtgatg ttaattctta ttggttttct actggaattg atgttagtac
    16261 tccaggagaa ttctgtttag aaagagatat agttactgat gttccactta cttctgatcc
    16321 tcttctttta aatatttatg gaaaagttac agcatcttat aaaactttaa ctcatttata
    16381 tgttggaact agtactggat atgtttttag tagagctgtt gattcttcta cttgggtttt
    16441 aatatctgca ataaccagtg attcaaatcc tgttactggt ttctttgaag tttcagatca
    16501 atattctgac tcagataaat atctttatgt ttgtaaaggt gaatataaag gttggatatt
    16561 aatagattct gagtggatgc aattagctcc tattatagaa tgggctcctt ctttaagtaa
    16621 tttagaagga gttaagcttg gaaactctgg aacattaact aaaagaaaga ttgctcaatc
    16681 tttcatttta agaagtaatg aaagagctaa aagattttct attgcttata aaactatagg
    16741 taattggact ggagatgtta ctgttaatat tcatttatca gatcctatta ctggtgcaat
    16801 agctgctgat tcaataattg aaacttttac agtaacatta cctaaaagct ctactatgac
    16861 atggtttaat tacgatataa gtgataccgc taaacagttt gttttaagta gatgtgataa
    16921 attctttata gaaattgttc ctttaacagc tgctaatgat actgcttgtc cagaatttgg
    16981 atgtactatt ggaagagatt cttattatga aggagatact ttaattgttt tagatcctgg
    17041 tgaagataat tatgcatttg cagttaaagg agaacaatta gcttttaaga tttggtctga
    17101 tttaccaact ggagttagtt tagttagttc tcaaggattt agtgcatttg gttgggttga
    17161 taatggaata aagagtactg ctaatggagt attatggtct cctgattctt caaaaggttt
    17221 atggacttta cctgcaagtc aaggaaaagc taaagcatta ggtagaactg gaagaggaac
    17281 tattgtgggg ggagccagtt ctttatggtt atttgttgga ggttctagtg ccatgaatct
    17341 ttgggatttt cctgattata ttaatgataa taattttaga ggatttcatg aatggaataa
    17401 taaaattatt ttctctatag aaaatcaagg aatattcatg actgatggaa gtactgttgg
    17461 acaatcaaat ctaaataatg aaagagaggc ttttaagttt attagttgta atggaataac
    17521 tacttctggt tgggatgcat ttgcactttg taaaaatgat aaaagtgaat ggtatttact
    17581 tagaagtaat gcttattatg atggatcatt tatttattgg tgggcagtta aaaagttatc
    17641 taaagaacca acacatattg cttctcttaa tgttaatact atattaattt ttaatgaaga
    17701 taaatctatt gataagtatt gtagaaatgg aacttatcaa tcaacaggat atttagaaac
    17761 ctctttattt gatgcaggat taattaaact tgataaacta tataaagata tagaagttat
    17821 gtttgaacct tttgaaactg atacatcttg ttctattgga tatagaactt ctatagaaga
    17881 taattatact acctctgaag tatttacatc ttctggaaaa cactctgtat tatatcctct
    17941 ttataatcca actagtggaa atagatttca agttaaagtt actttaaata gtaatacaaa
    18001 taaaacaaaa actcctattt gtagtgatat tgtttggtct tatatcttac aaagaagtgc
    18061 taatgaaaag aatacattaa aaactttcta tgttacaatt atttgtgagc cttctttaga
    18121 gaataatatt ggaaatgttg aatttattaa tgctgaagga tcaagatcaa gtaaagatat
    18181 tcttaatgat ttatgggaat tatcacaaaa gagagaagta ttaaattata ttggaatgga
    18241 taatgaatct aatgttgcct ttaaattaga atataatgga gagaaaaagt atacaattaa
    18301 aatagataga actaattatt tattctctat tttaaatgga acaactgtta aatatacttt
    18361 taattataga gataaaacca ctaatgatat agttgatgat ttaaatgaaa gagttttaac
    18421 tggagatact gaaatgatat tatgttctct tgttgatcca tttaaaggaa ctgaatcagt
    18481 taatatgtta gagccaatta aagatgttta tttaacaaat gaaacttatc ttaatattgg
    18541 aacagatgta agagcggtat tacttaatcc acaaagtcct agtcaagtta aaataagtct
    18601 tgataaatat ggaagagata gaattcaatt gaacttgaga gaagcaaatt aaaacagagc
    18661 tgcgatgcgg tgtatcgttt gaactccgta tatatgatgt atatacgcag ggcaattaga
    18721 ggttcgactc ctctctctgt gcatgagtag aaaaaaaaca tataacgaaa gattagcaga
    18781 aggagttctt gctggtagag gttatgaggt attagattca acaaataaaa gaaagaatat
    18841 tcatgaaaat attgcatatg atcctactct ttatattcca ggcaccttac cagaaaagat
    18901 agtccttaat catttaataa gtttacaagt aaactttaat tttcagtatc attttatgaa
    18961 taactatgct actagttatc cagaagatgt atgggttgct gatttctatt tagaagatta
    19021 taatacttat atagagattt atggaacata ttggcatagt acatctgatt ctgcagaaaa
    19081 tgatttaata aagaaagttt actggttaaa tagtggatat gagatattag aaaagggatt
    19141 atcaatgcat ccatctggaa tggcaacaat aggaaaagtt tgtatctggt gggaagaaga
    19201 aatatatcgt ggagtagctc aattatttaa tcgagattta ccagaagtca tgtctaatag
    19261 aattccagga aagatgaaag aactaacctt cgatgtatat gcagaatgga atgctaaaga
    19321 aaaaagagaa gcttctatca atttaaaaaa gataattcct aagagagtta ctcctaaaga
    19381 agattttaag aaattaagag atagaacatt agtacaaaat atttatggaa aatcaaaaaa
    19441 ccccagaaga atatacaggg gtaaagggct tagttaggtc tatttaccaa gaaacagcta
    19501 ctagttttgt tgattcaaat aataactatg ttactgtttt tcctgataaa atagaatata
    19561 taaaaactca ggatatttgt actggaggag ctaaaacaag tactggttct gtctctgcta
    19621 caggatctgg aaatataaat cctagtgcta tttctagtgg aagaattgct tcttatttaa
    19681 ctgttggaga tcttgttgca attgatggaa caagaggatt aatttctctt ggtattcctt
    19741 ctagtggagt agttggagat aattcatata tgtatatgaa tgcttctgga atatttggat
    19801 atcatggaac agatacattt ccttggtttt ttgctgctac taaagatatg gtttttggta
    19861 gtaggacata tcatggaggt gatttcttta taggagaaat agattcagct tatttatggt
    19921 ggcagtatga tgaacataaa ttaataattg caggagatat tagtatatcg ggggatattg
    19981 agtcaagtaa ctttgaatat gatcctttta ctggaacttt agttggttat aaattagaat
    20041 attatggagg gaatgcttat tttggaggag cgattgtatc tggtaatagt tttattactg
    20101 gaaatacttc tatttatgga aataatgtta ctattgctgg acaatcaatt gttaatgtag
    20161 gtaatgtttc agattcaaca gcattaacaa tacctgctgg attattagta gatggaacag
    20221 caataacaat tgctaatgat ggaacaatat ctgctaatat aacattaagt tggacggcag
    20281 aaacagatcc aagatttgat cattatcaaa taagatataa gaaatcatct tttacttatt
    20341 atcaatatat aagctcaaac tcaaatacaa tcacaataga tggacttaca cctaatactt
    20401 cttatgattt tgcagtgtca agtgtaaata aatatggaat accatcaaca tattcttcaa
    20461 ctgtaactta tacaacagca acatctacaa cagctccagc aaaagttagt ggtgtaagtg
    20521 ctgttggagg aatacaatat gttatagttg agtggataag taatagcgaa aaagatttat
    20581 cttcttataa tgtttatagg aatacagtaa ataattcaga aacagctact ttaattggaa
    20641 attgtaaaac taattatttt gtagatggcg gaagaactgg gggaacagaa tacttttact
    20701 gggtaaaagc agtaaatact tctggacttg tatcaactga tttttcaaca gtagcttctg
    20761 ctacaccgag aaatgtaaca aatgacgatg ttgtaaatct tgctagttct aaagttttaa
    20821 ttgatggtgt aacttattta tctaattgga gaaaatctgg agatttaact aaaatagatg
    20881 gtggctcaat atcaactggt tctataacaa caacacaatt aaactttaca ccagttattg
    20941 atgggactat tattgcttct ataaatgcct cagaagaagg aataaaaatt tctggagaac
    21001 atatagcaat tagtggttca actactttta gttctggtta tgnnnnnnnn nnnnnnnnnn
    21061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21121 nnnnnnnnnn nnnnnnnnnn nnatttctgg agaacatata gcaattagtg gttcaactac
    21181 ttttagttct ggttatgacc caattactaa ggtaaataaa cttggcggct cttatgcatc
    21241 agcatcttct ggagcaagag ttttaatatt tccagacacc aacactggtc tacaaataat
    21301 tgataatggt ggagcagatg tatttaaggc aataataggc ggaactaatg taggagatgt
    21361 tattatagga aattatacag gtggacaagg aatcttctat gataaaagtg aagcaaaaac
    21421 aatattcaag ggttcaatga tagcaggaaa tattactggt gtaacaatta ctggtggaac
    21481 aatacaaaca gcaataagtg gaaaaagagt agaaattaaa acagagggaa aatatgaaaa
    21541 tagtattgct atttttgata gcactggaaa cataggaata catctttttg ataactcaat
    21601 acttggtggc tggacaaact ctgcaataat atttgaccag tcatatggac ttagggtagc
    21661 atttccattt ccaacatcag accaaggtta tatatatttt ttaagaaatt ctagccaaag
    21721 ttctggaact gcagctccaa taataaaagg ttctccttct cttgctattg aagtaaactc
    21781 aataacatta agtggaacca ctagaacatc atggccatct tcatttagtg gaaatctaag
    21841 tgatttatat ataaatacaa caaaaagttg gggaagttat ggaataacag gtcttggaac
    21901 aataactcct tattccaaca attcatatag tttaggtagt agtagttata aatggaacaa
    21961 tgtttattca acttatggaa catttgatta tttaggtaaa agcctaaatg caaatagtta
    22021 tggaataaca ggtcttggaa caataactcc atactctaat ggctcatata atttaggaag
    22081 ttcaagctat tattggaatt atgtttatgg aataaatatt attccttctg gtaagtatac
    22141 ttcttattta ggaaactcaa gttatcattg ggattacttc tatgttgata caattcgtgg
    22201 agcaggtgga actgattcca ttgagataga aaacaatgca tttacaaggt gcctatttcc
    22261 agctgataat gttaaatatg atataggtgg tagtggtttt ttttataaaa atgtatttac
    22321 tcaatcttta agattaagaa ctgggcaatc aacaaatcca gcaaacgatg gagaaataag
    22381 atattatgat gtagaaggtg gtggttatag aggaagagtt agaggttatt taatgcaatt
    22441 tgatggaaca cttaaataaa aattatggaa aaatatacag ataaaaattt atcaaaagaa
    22501 gaaaggattt cagaatataa caaggaaata gataaattga tgaatcctat tcctgaagaa
    22561 gaatttgatc cacaaacttt tgatcctata aaaatagaag gaatggaaga tttagttgtt
    22621 agaaaaagag ctattaagaa aggaaaactt ccaacatatg gtttattacc aaaggagata
    22681 agagaggggc aaatgatagg gatgtatgaa tcaaaacaag atatttactt attattagct
    22741 aataaaataa atgaattata tgatgaaatc aaatcattat ctgatgaaat caagaaaata
    22801 aaagataata aataatatat ggcaaatgaa tatcaaggaa catcaattgt tgactaccta
    22861 aagtcaatag gtcaaaactc ctcttatcaa gcaagggcgt ctttggcatc tcaatatggt
    22921 agatcaaggg gaactggaat tataggacaa gatggttctt gtatcataac tccagagtct
    22981 cattttatat tagttacaga accagaagga ttggtaacag ttaatttaac tgctacagaa
    23041 gaagatcaag gactttatgt ttatcatata gaaaagaatg aatatattga agttagagga
    23101 aaaagtgggt catcttttat gtatgagata ttagcaataa gaaaaggata tttaaattca
    23161 aaggtcgaaa tagataatta aataaaaaaa atatgaaaat aaatgaatca ttaaaaacaa
    23221 ttagcgggaa ttactttaca ttagatgatg gcaaaacaaa attaacattt aaagatgcag
    23281 ctataatttg ttgtgagaca tataaatcag aaaaaagtat tcctggtcaa gcattaaaga
    23341 taattgcaat aggatctaag atctttaatg ctaaagatga agttgaattt actgaagaag
    23401 agaaggcttt attaagagaa gtaatagagg gaaataaagt ttatcttact ggagttacag
    23461 gagcaatgtt aaaattatta agttaacctt gtgtacttat cataaataag ataaaataaa
    23521 agaaaattag agagatgtcg actatcttta actattttaa aaagaactta ctgagtacct
    23581 tagagtatta cagccataat aataagtaat taaaaaaaaa tatatgatac caaataaagt
    23641 aacgggagcg ggaaacattg atgctactat ggcttataaa gttaagtttt ctaaggcatt
    23701 aagttctact ccaaaaatag aagcatggga taactcagaa acatttccag cagttggaac
    23761 tacttcaaca acaactaaag agatttttac tggaactgct ggaaatggaa acaaaccaat
    23821 gttagctgga tatattggtg gtcttgttgg tgctgctaca aagcctggtt caaattggca
    23881 tccagcatca gcaacagctg gtgcagctaa tccaaacctt ttaaagggaa atacgaacta
    23941 tgttatggct gcagttgttc cgggagaaaa tgacgagtta gtgtttaatt tatccttaag
    24001 agttccttat gatgcaacag ttccaagtac taattctatg aatgcattaa taagaattgt
    24061 atataactat acaggaatgg ctccagatat aacactagca tttaatgaag gaacagaagc
    24121 agttccatct tggacaagtt ttatagctgg aactcatgga attagattct gtaatgctga
    24181 agtagtggga acagattata aattaactct tccagaagaa ggtcaagtat atgctggaag
    24241 tcaatacgta acaatatgag aataagatgt acaaattgtg gcatttattt ctttagagat
    24301 agccaagacg aggtttatac tctttgtgat aagtgcttaa aagaattaat taaagaaggg
    24361 tctgctccag ttgttcctgt atcagcttct gctacacttt cttttactgg tgatattaat
    24421 caaggagata ctatcacaat aaatggaaga acgtatgaat ttgatattga tgggtcaatt
    24481 agaccaggaa atattccaat tgatgttact ggaaaagctg ttgcagctac tggaacatta
    24541 actgcaactc aaccagttgt tcttggtaag aaagtaacaa taggaacaga tgactatacg
    24601 attgatgatg aatcaggcga ttttaagatt aatgttggag ctggaactaa agttgcagct
    24661 actggaactt tatctttaac tggagcaatt actcctggta gtcatgctga aacagtacta
    24721 actagtactg gtgttgctcc tactccaata acacaagcaa ctaagatatt taattttact
    24781 ggtgtcgcaa gtgatggtga aacaattact cttggtgcta gaacctatga gtttgataat
    24841 gatagttcta ttttaccaag taacgtttta gtagatattt ctgcaggaac taaattccaa
    24901 gctacaacaa agttttcatt agcgcttcct atattagatg gtgaaacagt ctcaatagga
    24961 gatgaggttt acgaatttga tatagacgga ttagttactt ctggaaatat agcagtagat
    25021 gtttcttctt tttctacaat atctcaagga acaattacaa ttagtggcac tccaatagat
    25081 caagagaaaa tgacaattgg agctgttgag tatacttttg tagcagcaag atcaggagca
    25141 ggagaaatca caataaatgc taataatgat attcaaagag ataatattat ctcagctatt
    25201 aatgctgatt ctacagatgt tactgctcaa tctggtgggt ctggattagt tacagtgtta
    25261 gcagctgagg atggtgctgc tggtaactta ataacattca cagaaaacgt aacaggaatt
    25321 gctatgaatg gagatggaac acttggcgga acacaaaaag ctattgattg tgatgcaatt
    25381 catgcggtag caacattgtt atctactttc aatgctaata cagcgtatga tataactgga
    25441 gtaccttatg gaactaatgc tatagacttt acagcagatg ctgctggagc tttagatgga
    25501 tcacttggaa atatatcagc ttcaacaaca acagatggaa ctataagcaa tttctctgga
    25561 ggagcagatg ctactggaat agaggctgca actgctttag ttgctacagt tgctgctgat
    25621 ataattgctg caccagaaat agttaatgta acagcagaag ctggtggtga tggagagttt
    25681 acagcaaaag ctttaacagc tggaacagtt ggcaatgcta tagtaattgg agaaactaca
    25741 gctaatggaa catttgctgg tggagcaact ctattatctg gtggtttaga agctgataca
    25801 gttacaattg gatcaataga atatacagct gtagcgtcat tatctgcccc tacagtagct
    25861 aatgaagtta tgattggtgc tagcgcagct gaattcttag ataatcttaa atctgcagtt
    25921 aatcaatctg ctggaagtgg atctgcttat agtgttggaa ctgtaaaaaa tccaatagta
    25981 gtagccacag ataatacaga cactactcaa aagtttatag ctagagaacc tggaactgca
    26041 gcaaatactg ctgatacaac tacaactgca gtaacattaa gctttgaaga tactacactt
    26101 ggaggtggaa ctggatcaag tgttccaggt gttaatgcag aaactgtagt tattggagga
    26161 aaaacttatt catttgttga tgttttatct gaaacaaatg gagcaactgc aatagttaat
    26221 caagttcttt ttggatcaga ttcttcagta gcattagaca atttaaagtt agcagtaaat
    26281 gctgaaggaa ctgcaggaag tagttattca actggaacaa ttaaaaatgc agatgtattg
    26341 gctactacaa atgaaagtaa tgctcaaaca tttgaagctt tggttagtgg ttctgcagga
    26401 aatgctataa ctacaacaac tactattgta aatggaagtt ttggaggagc aactttatct
    26461 ggaggttctg atgctactgc agcagaattt atagctgcta tagtaacaac tataaatgca
    26521 ataacaggaa atatagttac agcagaaaat gaaacaggtg gagttatgaa agtaacagca
    26581 gctgttgctg gtgctgctgg aaattctata gtaacaattt caaatgtagg aggagcaact
    26641 tttggagctg caacattaac tggaggtaaa gatgcaggaa gtacagatgc ttgtgcagaa
    26701 acaaaagcaa caattaatgc agatactgta tcagttgttt ctgctgaaac tatagatgct
    26761 tctagtataa agatagttgc aaaaacacca ggagatattg gtaatataat taaagtatct
    26821 actgatggag ataatattgc atttgataaa gcatctttaa gtggtggagt taatggaaca
    26881 cctggatgga ctaatcaaat attatcagat ggatcaaaat tatatatctc ttcagagtta
    26941 agtacatcta cagatagtaa ttggaaagaa gtagacataa atgaagttga agaataataa
    27001 gtaaaaaaaa tatgagaata caatgtttaa gatgcggttt aatgattaca gtagatcagc
    27061 aagatatagc tatgcatcct agattatgcg ataaatgtct tactgtagct gttcaatcat
    27121 caggagaaga aatagaggat gaagaagtaa aagaagaaat tactcctgaa gaattaggaa
    27181 tagaagaaga agtagttaaa aataaaggtc gaaatagata attaaataaa aaaaatatta
    27241 tgatttttga tgatgaaatt gaagaagaag gaattgaaga agaagtagaa atagaagaag
    27301 aagtaggagg tgaagaagaa gaagtagaag agtaaactaa agatctgaat taatatttaa
    27361 caattataga tctatgttta tagttcaaac taaagacggt aaaacttttg ttgaaggcaa
    27421 agaaggattt gtttgggata atattccaga tgatgttgaa ataacttctt tgtctttaac
    27481 tttacctttt aaggtgagtt ttaaaactaa gagcggtgat attcttttaa atcctaagtt
    27541 cacaattaag gattttgatt catacttttt ttctaatgag gagactataa gcattcttgc
    27601 tgttaatagt atattaggaa aaagtaatag agtattaact gctaaaataa taggaggaat
    27661 aaaaggagat aatgtttttg aatatagaat ggatagacat ggaaatatta agagcagaat
    27721 cttttctttt tctgaactag aaaaaagcta taatttgtct gctattagaa aaggcttgac
    27781 taactagtgt tcctattgat ataatgaaaa aaataaacag ctgagaagtt gccaatatat
    27841 tgataggtag aacacttata gttatattgg ctaaaaagac tacctacaaa taaggtagtc
    27901 ttttaataaa aaaaataaat gttagacgca gcatataatt tcgcaaaaac aacaatctct
    27961 ccatttggtt ctggagcaac atcagctact ttgactgatc ctacaagatt tttagatcca
    28021 tcaaccaaac aatataattg tgttgtttgg tgttcttctc tttatcctaa tccaatagat
    28081 gatcctaata aagagatagt aagatttact gctaagagtg gttctgttta tacttttact
    28141 agagcacaag agggaacaga agcaaaagat cataatatag aagatgaaat ctattctgtt
    28201 gctatagtat taacagagaa aatgatttct gatattcagg atgagcttga tgataaagaa
    28261 ccagtactta caaaaggaaa tctatctggg tctggagtaa taacaataga taacacaaga
//
LOCUS       DAOPCH010000003        24920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_2157_length_24920_cov_4.720129, whole genome shotgun sequence.
ACCESSION   DAOPCH010000003 DAOPCH010000000
VERSION     DAOPCH010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 24920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24920
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2157_length_24920_cov_4.720129"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLU55_00385"
     CDS             <1..416
                     /locus_tag="PLU55_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86553.1"
                     /translation="WKFQEIVLDPRKMKYDWCKGETSPMILFPKDFISLKNKDLVVGI
                     INGREVKKIVNKEKIYGKFCPGLIIYNPETGKIPWIAPECLFEDPDARTITFGSDFLR
                     TEKQSGILYAHVNDSFIRAYKIDANELKKLLPKNF"
     gene            complement(413..1522)
                     /locus_tag="PLU55_00390"
     CDS             complement(413..1522)
                     /locus_tag="PLU55_00390"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 100 family protein"
                     /protein_id="HPJ86554.1"
                     /translation="MNEKLNLAYKKAIETVNECSNKYGLFASGGKRGYRGVWSRDSMI
                     TLISASCTSLDSKTIKEAFKRSLITLAKHQSKLGQIPNAVHGFDTNKINVDFQSIDSS
                     LWFILGEYYYKKFYGNELFVKHKDNIKRALTWLSYQDMGEDGLLEQLPTTDWQDAFPH
                     RYGHTINTQALYYHVLSFVGDKKEQERIKVSIEDEKNTGLWNGEFYYAYRWKNHNKYK
                     EIGEWFDSLGNLLAIVFGLADEKKSNSILDYISDKKINEPYPVKTIYPPIEKGSKYWQ
                     DYYLDCKAGTPNEYLNGGIWTFIGGFYVLALIKVKRFKEAEKELMKLAEANLKGNYPE
                     WINPLNKRTYGKYQAWSAGTYIAAYESFKAKKVLI"
     gene            complement(1519..2595)
                     /locus_tag="PLU55_00395"
     CDS             complement(1519..2595)
                     /locus_tag="PLU55_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86555.1"
                     /translation="MKKGVKLLITPEMIKPSSKYLKVIGTINPAAVRARNGKVVLYVR
                     VIEQLKKLEDKGYFYSPRMSGKSKYELKIDRFSKKIVENSSEFDILFKDGTKRLTFIS
                     HLRRVILDESGMNIISIDKKPSFYGIAHDAELGVEDPRIVEIEGRYVMTYVSLSRKEN
                     ISTSIAFSDDLTNWERKGIIFGEQDKDVVIFPEKIKGWYVAFDRPESNFQFSPPHIWV
                     AHSDDLLCWGKLKSVSVLNEGEKDYGRIGPGCPPIKTKYGWLLIYHTVVEEDKLFTYS
                     ASVALFDLNNPRKMISNSEPIIIPNMDYELELYQNKKIVFPTGIVVDKNGRDLLIYSG
                     GGDVVTSVRKLSIDKIMKALKKVK"
     gene            complement(2687..3364)
                     /locus_tag="PLU55_00400"
     CDS             complement(2687..3364)
                     /locus_tag="PLU55_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPJ86556.1"
                     /translation="MKIIEVKNYKELSRIASDIIIEEINKKPNIVIGFASGKTPKNTY
                     KNLVKAYKNKKVDFSKITSFNVDEFYPIKKESKKSYYRYLFTNLLNKVNVNKKKIFLI
                     NGDTKNSEKECFNYEKRIKENPIDLQLLGIGENGHIGFNEPGSSRNSKTRVVKLTHIE
                     GTAITMGIKTIMKAKKIILLASGKKKAKAIYGLVKGKITEEVPASFLREHKNFILIID
                     RKAGSLL"
     gene            complement(3361..4485)
                     /gene="pgk"
                     /locus_tag="PLU55_00405"
     CDS             complement(3361..4485)
                     /gene="pgk"
                     /locus_tag="PLU55_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPJ86557.1"
                     /translation="MKTLNDFDFKGKIVLLRSDLNSDVQNKKLLKSERISEASITIKE
                     LKKKKARVVVLAHQGRPGDEDFISLKQHCKQLNKYVRVKFVGDIIGKRAEREIKKLKD
                     GEAILLENVRNLKEEFDSNPNKLTEFFSKNCEIYVNDAFSVCHRAQASILLPKYIVNS
                     CAGRILEREVNALKKLKLSKTLYILGGAKPEDNIKLLGKNKVLSCGLFGQVCLINKGK
                     KLGEQENYLRSRKIFIKIPRVKLKNVETPLDFAVNIKGKRKEIPVEGFPNDNEIFDIG
                     DITQKKYIVEIKKAKAIYMKGPAGDCGKKQFSNGTFAILKAIAGSKAFSVIGGGHLSD
                     AIKASKVNVKKFGHISLSGGALLNYIAGEKLPGLEVLGIK"
     gene            complement(4515..5498)
                     /gene="gap"
                     /locus_tag="PLU55_00410"
     CDS             complement(4515..5498)
                     /gene="gap"
                     /locus_tag="PLU55_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPJ86558.1"
                     /translation="MVKVAINGLGRIGRSFLKIALDKGINVVAVNDLASIDSIVYLMK
                     YDSVYGNYKGIIEKGNGFIKLNRKKIMVFSEKQPENLPWAELGVDIVVECTGAFTDRE
                     GAMKHLWAGAKKVLISAPAKEPDITVVLGVNEKELKKEHKIISMASCTTNCLAPVVKV
                     LNDNFKIQSGYMTTVHAYTNDQEILDIPHKKFRRGRAGAINLVPTSSGASSSVGEVIP
                     ELRGKLDGLAIRAPVPCGSITDFVAVVGRAVTREEVNAALKNAAEKQMKGVLQYSEDE
                     LVSSDIIKNPNSSIVDSLLTQTNGNFVKVLSWYDNEWGYSNRLVEMIKLLK"
     gene            complement(5791..7449)
                     /locus_tag="PLU55_00415"
     CDS             complement(5791..7449)
                     /locus_tag="PLU55_00415"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPJ86559.1"
                     /translation="MAYVVKQKINGKEYFYLRKSVRKGNKVTSQHIAYLGSNKEEADK
                     KYAEIIKNMDSNNQEIKMEDKSNIKMENNLIQKKISIDELAKFCKEKGFVFRSSDIYG
                     GYSGFWDYGPLGVELFNNIKRDWWNFFVNKRDNMVGMEASIISHPKTWKASGHIANFN
                     DVAVVCKKCKTATKIDKSEVGKVNCKCGGEFDIRGEFSLMFKTKVGALDSSDTYLRGE
                     TAQGMFLDFKLISQTSRMQLPFGIAQIGRCFRNEIAPRDFLFRSREFNIAEFEFFLNP
                     EEKLCNDLEENHLGIKLKLLDAETQTAGKEDLKETTIGQMLSEKRMEEWQAYWLAEQI
                     KWYHSLNLFEIKIREHTKDELSHYSSATFDIDYEYPFGNVELGGNANRGQFDLTQHSK
                     ESGENMEVFDEKYKNKIIPRVIEPTFGVERVFLAILTKAYSDDGRGNIVLNLPARIAP
                     VKAAVFPLVKRGDFEKIADDIVKDLREEFNIAYDRSGSIGRRYARNDEIGTPFCITID
                     DDSLKNKDVTIRDRNSRVQKRVKIEKLKEILRDLISEKLNFQEI"
     gene            complement(7516..7587)
                     /locus_tag="PLU55_00420"
     tRNA            complement(7516..7587)
                     /locus_tag="PLU55_00420"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7553..7555),aa:Cys,seq:gca)
     gene            complement(7595..7669)
                     /locus_tag="PLU55_00425"
     tRNA            complement(7595..7669)
                     /locus_tag="PLU55_00425"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7632..7634),aa:Arg,seq:tct)
     gene            complement(7744..8646)
                     /gene="rnz"
                     /locus_tag="PLU55_00430"
     CDS             complement(7744..8646)
                     /gene="rnz"
                     /locus_tag="PLU55_00430"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HPJ86560.1"
                     /translation="MASKIKVIFLGTSAQIPSAKRNHTSILLTYKDENILIDCGEGTQ
                     RQFRKAQLNPCKLTAILITHIHGDHTFGLPGLISTLNFSGYNKHLKIYCPKGTKKIIE
                     DFIDLKHLKNGFKIKVEEVIGKFYENEEFYLEAESMQHGIPTNAYNFVLKSKHRIDKS
                     KLKKYKIPEGPLLKNLKQGKDIVYEGKKYKAKDLIYIEGGKKISFVLDTAFNNKIVKF
                     VKDANLLICDASFSSKNSKEAKEHMHLTASQDGKIAKSAKVKKLVLTHISQRYDNKLN
                     ELFEDAKKEFKNVVIAEDFSSFEI"
     gene            complement(8771..9022)
                     /locus_tag="PLU55_00435"
     CDS             complement(8771..9022)
                     /locus_tag="PLU55_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013683906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86561.1"
                     /translation="MANGIERPLDLLNTSKGKEVLVHLKGEKCFVGTLLAFDIHINLV
                     LDNVKEMQDGEVKKSLGLTFLRGDTIIYISPASTALKKD"
     gene            9121..9486
                     /locus_tag="PLU55_00440"
     CDS             9121..9486
                     /locus_tag="PLU55_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HPJ86562.1"
                     /translation="MKLGRKITGGRYIKSMKKKKHSLPGQKKTVKLAADEKRKNKRTR
                     GGNRKNYLLKGKFVNIQEKGKKSKKAEIKNVLETPSNKFLARQNILTKGTIVQTDLGK
                     VKITNRPSQEAMINGILIE"
     gene            9613..10548
                     /locus_tag="PLU55_00445"
     CDS             9613..10548
                     /locus_tag="PLU55_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HPJ86563.1"
                     /translation="MAGIRKCPECESINLTYDSHLGEIICNDCGLVVEEKMVDTGIDL
                     SGKFDKSEKKGRGGAPISIQKFDKGLTTNVGEISDIYRLEPKQTRKFLRLKKWQERVS
                     TSIERNLRLAMAELRVIASYLNLPNVVRDEAARIYNYVLQRGLVRGRSMESVIAACLY
                     AACRSYNIPRTLDEMATASDVERKEIGRTYRFIIRKLGIKVNQSSPKDYVSRFSSVLK
                     LSPKTQNDALKVLKQAEEVELTSGRGPAGIAAAALYVAALMNDEKKTQREVADIAGIT
                     EVTIRNRYKELIEKLKLEDKIKQKEADSKKKKTTG"
     gene            10628..10710
                     /locus_tag="PLU55_00450"
     tRNA            10628..10710
                     /locus_tag="PLU55_00450"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10662..10664,aa:Leu,seq:tag)
     gene            complement(10754..18790)
                     /locus_tag="PLU55_00455"
     CDS             complement(10754..18790)
                     /locus_tag="PLU55_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPJ86564.1"
                     /translation="MKKINQSFKILIFFLFGILLFVPLISAVYTESLTYSSKYNRAIG
                     QGVFSTGESLRIDESMCQQGTDFIIQINPLECIPSPVRSDLLEEENVNVFCKLTAIKI
                     NPFIEVTDVDGIRFDREYPREVLNIGYQPAQAALGYTEDNLDGSLINNNFGYVVITLR
                     RQPNESALTNCEKAAFGLSEVCWVEGNITATLRYDVRNSFGLGNAEFYLPVMNDKEWE
                     DNYLYYGFWDGRGYLRAESVTEDSAILSIYSASSVARPSGKGGFDYRLARYASNIKLG
                     IGDSSRKVFLPGINPCLGNLQLTLHGIERPDTAVMFKVNGDYVEVRKDGKFLDERCTV
                     IDIFKTGLSSQVKIRCKEKKQKVYTLSLSPQVNLSLDGGNPKEYSIGDFLYGYTDSKG
                     QYQKVYLGYIGSDGNEEDLNDLYVILVSLPISENIKKNSLDEEELYFIASYAEAKDGF
                     LSNLGVNTVRFFKWAITGESFKVVPYKNKKTGAISEGIEFADKKVALRGFAVGNYLDV
                     VQSDEELYQSYYDSAIEDFEKVIDSYSNEREYESDIQDFGERALIEEIYLAESVKEMR
                     TVLELCKKFNEEYPNSQARIGICDSNILLSNNKASIVEVTINDITNRISFLGVKEPSL
                     EEYNAEVVVKGPNGRTERYNLRKNKQIPLIGFRTEEDTQKLDSGEYILLESLDTGSAQ
                     ITFAVGKTTTNNKGDKEITVSTKSEKLNLNVQRPDIGDYSFTLVDTTLASVAKVSIDS
                     NIERAISNSSFKFKIGIEKRAIKLAPDKTKEKLRELEKRIDDLTKISSTLDSINRGMK
                     FACGATGAGLVVKNLIANRNGKAIAREKVMAGTGGWNEKCQEDYNSGLYKYKSVEDCL
                     LDNAEEIEKEVEDMANIISQQNNKIRELEATYTKDGKLNEKLFMVSYSNHVYSSVDSS
                     LSKMDRNSLISLTESLHKDSLSPDEIMGMSEEKMIELNRQKIQSILNNRGTSESKYGT
                     DYLVSEGAFTVNELKEIELYATYLNKNSGDIEASSRLSSAMSKVGINSDKYLNALETA
                     KTLGGVNPSHIVYIELKDENAVKYDYRGNTKGNTKTNLPDTIPKDTPIVITTIPAIEK
                     GNLVFVLEKSKGSGVYPIDKVYYTDGTEFTDKEILSKLRREVTIVKRDSLYKNRYLSS
                     YGSAKTGPLIKFFESSSHSCRPALVPLDTHNGWYAGMKSAEMPYYSDSGRIEMFYLCN
                     VGDNGIEEFSVSGFGDDFCTLLTAAGLSSSQFSQLEGKSIQDYFDLAVRSIAQAQQTC
                     MRGISPGSSVNINGNSLNIGEPPSEDFGFRCTDFMSVKDCNLLFNLCDPVICPSSRCD
                     LGGKYPVKNVISSGIIGSIFLCLPNWKVTGGDVYIPVCLTGVQAGIDNLISVQKAYSD
                     CLEKSLETGETVGICDEIHSIYLCSFFWEQATPIIKYFGSHLLERILGQKSQGGGEYK
                     GFASALKNSQNTVDYLKNYYAKESYRAFQVRSMDAVRTEACGRFFSAVYADSGSFISN
                     LIAPDSPSQFTGKFEEIPLTTSTNPPTSHYKVWYHIYAGKDSGAYYRVYLRGSASNYY
                     MDNVQRRVVNSSYIPKGGYADATIDFTAPAGYRELCIEVNGVEECGFKEVSTSFSVNY
                     LSDLFLKDQVKKKNIDSEEDCRSGTASIYSLLDLNVLSAAENLINPDLYAQGITRVCS
                     THNPGLGPEISSSDSEDLRWQKVGHCGDERVGCWIDTESVRKAIKNLNLEEDAIESIK
                     DETLSYLSEDYFTQKEFEAKKKDIQSEDDPLKRIDLIDSIFEIDKIFFNNHKAYLFFW
                     RGKSYAQLAMNNFGRCATNGNTKCEGTTHFVCEDNKWVNKGQVNGKCGYVEDSTTTSG
                     GDSWFNIKYTSPHLLPSDKNILTLDDRCYRYFNSKWQFAISCDPTVAWFNVDVLGNEY
                     TNPVSKRSKEIITSLQNYNGDYLGGLKVLIDKTMEDRNAKLSEKTFKVSSMDEEGLFT
                     VKFKDVNYEPMKKIYYKYEDGEWYWELDKMFEIGGVRHSVSNVAPGFNNLQKAIVLAL
                     EGKTFYEGAAILFNPTSKDIQERYFVGGSSTTSVATSLEANLQKRDLDKVTKLMKDLS
                     SSVYASTFKCYCGKECENYAKWIFEAANSNNIPDELLLLAIMIQESSCKAVKSSDGGD
                     IGLMQINVLHCGTKGLSSNSGNCISTLLNDNEKNIKIGAQILKEAYSPSSRVYTCVMP
                     VETYFGWEYALRGYNGWNTDCSKGDIHYVRNVKEKYFELVRLYNGGTPTTPTTPTTPT
                     TPSTPSTPTTPTTPSTPSTSDVYKHMMFSPINPHSSVVFYRYNTVWQWNVYTKSISDS
                     SWESVPSYTIKGGSYDGKTLNDKNQELILKLNGKNYEEGLKILKEFGLITLDGDEIRI
                     PSIPPTDCDYEKINPPCTSYKDVQLSMFSTAERKLFLAVKNCEECGDGLSNLCDRDEC
                     KAIALKLNKNCVHTNLVLGYGKCEEKSSTAPTTPTTPSTPTTPTTPTTPSTPTSPTTP
                     STLSPIRQKVLTSAQELDGTIVPEVYNNNCGVAANYIYQHASAGLSRCIYSTKKGTEY
                     SIFLRDGTTAKVGIGSQKDNAGRIIWVTADSCTYSSDTLTYSDLLKSLVPGDRIDIVY
                     NEDSPHSVVFINWVKGNEYIIANVSDWNGKTLIKNQKDRVGTVCTEKDLYWYAPRCKI
                     YQYTQYDLRVDRHPIYRVWNPVPI"
     gene            complement(18787..>20300)
                     /locus_tag="PLU55_00460"
     CDS             complement(18787..>20300)
                     /locus_tag="PLU55_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86565.1"
                     /translation="PSKDSMEKNLEMFMETKSRACFFDDYYSQGYWIDMEKPKAKITI
                     KDHEVVLDLKMDLIISRENENHVIREHKVVLDSELGSLYESAIKVYEEEQSSLFLEDY
                     AIDILRLYAPVDGFELTCSPKVWIANEIFRDLREAIEANTLALKNSNNKEDYFEVEIS
                     DIPSNHRVRFLNSKDWTYSFEVNPSDGPVMTANPVGNQPGLDILGFCYVPYHFVYSLR
                     YPVLIQIISGKGSEEIFQFPMAVVIERNLPRNASGESVPLIDSQELCTNKNTYMNIQV
                     YDTNLKPIEAYVSYSCLGSTCSIGTTKNGRISEKFPQCINGFINVKSEGYKPESIMYS
                     TVKEGVMSVYLSKLYNLSVQLKLNNQNYNRDAIIYFVSDDFSKTIYYPRQKYVELAEG
                     EYEVQVYVYKNSNLKVGNMTQKQCVEVPRSSIGGLIGLTKEVCFDVQVPAQTLSNALS
                     AGGKLNYTFSENQLRGSRVIDIYAETLPEPNTLEQIQKNYNLFENKELKINLR"
     assembly_gap    20301..20400
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20514..20720)
                     /locus_tag="PLU55_00465"
     CDS             complement(20514..20720)
                     /locus_tag="PLU55_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86566.1"
                     /translation="MARGGLFFFVLYLVIGAYFLNFGLNFIKLPEIAANINRWIIFAG
                     GILLIIGAVNYLRANRASRYNRAR"
     gene            complement(20905..21246)
                     /locus_tag="PLU55_00470"
     CDS             complement(20905..21246)
                     /locus_tag="PLU55_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86567.1"
                     /translation="MVPQLYLGNHLVYFKLPLLKSNKMRVKFSKGQQREFFKKVMETI
                     NCPSLRELINRGIDVNYSTLKNYYNEERLIPEYLFKELIGISGINISDFKFELIEENW
                     GKVKGGKISRR"
     gene            21288..21599
                     /gene="rpl44e"
                     /locus_tag="PLU55_00475"
     CDS             21288..21599
                     /gene="rpl44e"
                     /locus_tag="PLU55_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="protein component of the ribosomal E (exit) site
                     that binds newly deacylated tRNAs after peptide bond
                     formation; contains a zinc finger motif; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HPJ86568.1"
                     /translation="MKIPKTTKRYCPYCKKKTEQKIKLVGTGFQRGTLTRGSKYRAKL
                     RGLARGIGNKGKWGSKPAASKFKRKSKTTKKTNLMYTCKKCNKSKYQKKGIRLSKQVQ
                     E"
     gene            21634..22290
                     /locus_tag="PLU55_00480"
     CDS             21634..22290
                     /locus_tag="PLU55_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86569.1"
                     /translation="MAQAKRKKKFFDVEMPIINKQTQIFAYELAELEGKYIRYDLTRI
                     LRGKSILMQFRVQVKDNKAIAIPRGAILMPYFLRRMIRKGTDYVEDSFIAECKDASLK
                     IKPFLITRRKVSKRVRRGLREKAKQEIIKYLKDKSSQKIFEEILKNKMQKDLSILLKK
                     IYPLALCEIKSIEIKKDLEVKVKEKPKEEKQEKEEDIQEKKEKKSKKKEEKEADSKEN
                     "
     gene            22431..22502
                     /locus_tag="PLU55_00485"
     tRNA            22431..22502
                     /locus_tag="PLU55_00485"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22463..22465,aa:Thr,seq:tgt)
     gene            22557..23177
                     /locus_tag="PLU55_00490"
     CDS             22557..23177
                     /locus_tag="PLU55_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86570.1"
                     /translation="MVKKEGITLNKKVIQEAKKRHRDAIDTWKKNLEEAIITSYNHIK
                     KYPNSSEKKFESVKLLLKDGLALLEDRTYKENFSEGREIFYYMKDAIAKKESKAILSN
                     EIDKYRQDLALRGGLLAEKAHLKMDFVDGDAKILHYYDKYFGNRSPILEKSITIVSLL
                     SILLGIFIGYPALTGNLIAETVNGSLAYGAILFFVGLLGVFLANKR"
     gene            complement(23205..23519)
                     /locus_tag="PLU55_00495"
     CDS             complement(23205..23519)
                     /locus_tag="PLU55_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007539921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HPJ86571.1"
                     /translation="MIVSTTETIPGRKIVEVLGVVKGNTVRTRNVGVDIGAGLKNLVG
                     GEVKSYTSMMTQSREEAYNRMINQAIELGADAVVGVRFMTAMIMMGASEMLSYGTAVK
                     LA"
     gene            complement(23533..23733)
                     /locus_tag="PLU55_00500"
     CDS             complement(23533..23733)
                     /locus_tag="PLU55_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86572.1"
                     /translation="MKNERVIIGGIIFSIGLIWTIVCFFTLLFMLIYSIPILIIGLVI
                     ILNKKEDEIEQRQDLNKLKTKR"
     gene            complement(23765..24325)
                     /locus_tag="PLU55_00505"
     CDS             complement(23765..24325)
                     /locus_tag="PLU55_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86573.1"
                     /translation="MITYNDIYEAARKERYSEQLQQIPKNFVSEFSKYLKEKKEVASK
                     EEEDFSDTAIKTKKQLENATTLFKELIIRRRKKILNLVLIAAETGISKQDFENMLAIE
                     KELFEGLMRCMDYSEKRLNESLNGIGKGELVKNNDLVVFLEDVGEFLSADEDKMGPYK
                     KGDIANLPKEISKILSEGKKVEILEK"
     gene            complement(24404..24724)
                     /locus_tag="PLU55_00510"
     CDS             complement(24404..24724)
                     /locus_tag="PLU55_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86574.1"
                     /translation="MENNNKTLVNKIPWTEKSEDVFDSDKEGSLFYRCQQLENEGFGM
                     LASSFDISVIDPSNSVVCPDGKIFTLKQIDDYWHRRRGSYSSRNPDDVRMHKVRIYYL
                     GFKR"
     gene            complement(24734..>24920)
                     /locus_tag="PLU55_00515"
     CDS             complement(24734..>24920)
                     /locus_tag="PLU55_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86575.1"
                     /translation="KIKDLRDEFLKKLKGKIDGTEVALSIASGEGKEHMALISALINL
                     PVGIRFAALTKEGVIDL"
BASE COUNT         8688 a         4606 c         2889 g         8637 t
ORIGIN      
        1 cctggaaatt ccaagaaata gttttagacc caagaaaaat gaaatatgat tggtgtaaag
       61 gcgaaacctc tccaatgatt ctttttccaa aagattttat ttcattaaaa aacaaagacc
      121 tggttgtagg aataataaat ggaagagaag ttaaaaaaat tgtaaataaa gaaaaaatat
      181 atggaaaatt ctgtccagga ctaataatct acaatccaga aacaggaaaa attccttgga
      241 tagctcctga atgtttattt gaagacccag atgcaagaac aataactttc ggttctgatt
      301 ttttaagaac agaaaaacaa agcggcatat tatatgcaca tgtaaatgat tcttttataa
      361 gggcttacaa aattgatgca aatgaattaa aaaaattatt acctaaaaat ttctaaatca
      421 aaaccttctt agctttaaat gattcataag ctgcaatata tgttcctgca ctccaagctt
      481 gatattttcc atacgttcgt ttatttaaag gattaatcca ttcaggataa tttcctttaa
      541 gatttgcttc agctaatttc attaattctt tttcagcttc cttaaacctc tttactttga
      601 ttaaagccaa aacataaaat cctccaataa atgtccaaat tcccccatta agatattcat
      661 taggagttcc agctttacaa tcaagataat aatcctgcca atatttactt cctttttcta
      721 tagggggata aatagtttta acaggataag gttcatttat cttcttatca gaaatataat
      781 ctaatatact attagatttc ttctcatcag ctaaaccaaa aacaattgct aaaagatttc
      841 ctaaactatc aaaccactct ccaatctctt tgtatttatt gtgattcttc catctataag
      901 cataataaaa ttctccattc cataatccag tatttttttc atcctcaata ctaactttaa
      961 tcctctcctg ttccttttta tctccaacaa aagataatac atgataatat aatgcttgag
     1021 tatttattgt atgtccatat ctatgaggaa atgcatcctg ccaatctgtt gtaggcaact
     1081 gttctaaaag cccatcttct cccatatctt gataactaag ccaagtcaaa gctctcttta
     1141 tattatcttt atgtttaaca aacaattcat ttccataaaa ttttttataa taatattctc
     1201 ctaaaataaa ccacaaactt gaatcaatag actgaaaatc aacattaatc ttattggtat
     1261 caaatccatg aactgcattg ggtatctgtc ctaatttact ctgatgtttt gctagagtaa
     1321 tcaaacttct cttgaaagct tcttttattg tcttagaatc caaagaagtg caacttgcag
     1381 atattaaagt tatcatacta tctctagacc aaactcctct gtaacctctc tttccaccag
     1441 aagcaaaaag cccatattta ttagaacact cattaacagt ctctattgct tttttataag
     1501 ctaaattcaa tttctcattc attttacttt cttaagagct ttcattattt tatcaataga
     1561 caattttcgc acactggtaa caacatcccc tccaccagaa taaataagca aatctctccc
     1621 gtttttatct acaacaattc ctgttgggaa aacaattttc ttattttgat acaattccaa
     1681 ttcataatcc atattaggaa taataatagg ttctgaattt gatatcatct tccttggatt
     1741 atttaaatca aacaaagcta cagaagcaga ataagtaaat aatttatctt cttctacaac
     1801 agtatgataa atcaacaacc aaccatattt tgttttaatt ggaggacatc cgggtccaat
     1861 ccttccataa tctttttctc cttcattaag aacactaact gatttcagtt tgccccaaca
     1921 aagtaaatca tctgagtgtg ctacccaaat atgaggagga ctaaattgaa aattactttc
     1981 aggtctatca aatgcaacat accacccttt tattttttct ggaaaaataa caacgtcttt
     2041 atcttgttct ccaaaaatta tccctttcct ctcccaatta gttaaatcat ctgaaaatgc
     2101 aattgaagtt gagatgtttt cttttctaga caaacttaca taagtcataa catatctccc
     2161 ttctatctca actattcttg gatcttcaac tcctaattct gcatcatgag ctattccgta
     2221 aaaactcggt tttttatcaa tagaaattat attcattcca ctctcatcca aaataactct
     2281 cctcaaatga gaaataaatg taagcctttt tgttccatct ttaaataaaa tatcaaattc
     2341 ggaactattc tcaacaattt tcttggaaaa tctatctatt ttaagttcat atttagattt
     2401 accagacatt cttggagaat aaaaataacc tttatcttct aattttttaa gttgttctat
     2461 aactcttaca tacaaaacta ctttaccgtt tcttgctctc actgcagcag gatttatagt
     2521 ccctataacc ttcaaatatt ttgaagaggg cttaatcatt tcaggagtta taagtaactt
     2581 cacacctttt ttcatttttt acacctacaa gataacatgt cctcgatagt ttttaaatat
     2641 ttagaaaaaa atattttaaa gcatactcct aatatttgat aaaaatttac agtaaagagc
     2701 ctgcttttct atcaataatt aaaataaagt tcttgtgttc tctcaaaaaa cttgcaggaa
     2761 cttcctctgt aattttccct ttaactaaac cataaatcgc ctttgccttt ttctttccag
     2821 aagcaagcaa aattatcttc tttgctttca ttatagtctt aatacccatt gtaattgctg
     2881 ttccctcaat atgggtcaat ttaactactc ttgttttaga attccttgaa cttccaggct
     2941 cattaaaccc aatatgtcca ttctctccaa tacctaataa ctgtaaatca attgggtttt
     3001 cttttattct tttctcataa ttgaaacact ctttttcaga attttttgta tctccattta
     3061 taagaaaaat ttttttcttg tttacattta ctttatttaa tagatttgta aacaaataac
     3121 gataataact cttcttactc tcttttttga ttggataaaa ttcatcaaca ttaaaagaag
     3181 ttatcttaga aaaatcaact ttcttgtttt tatatgcttt aactagattt ttataagtgt
     3241 ttttaggagt ttttcctgat gcaaatccaa ttacaatatt aggtttttta tttatctctt
     3301 caataataat atcagatgca attcttgata attctttata atttttaact tcaattattt
     3361 tcattttatt cccaaaacct ctaatccagg taatttttca cctgcaatat aattaagtaa
     3421 tgctccacca cttaaactaa tatgtccaaa tttctttaca tttactttag aagctttaat
     3481 agcatcactt aaatgtcctc cccctataac agaaaaagct ttgctccctg caattgcctt
     3541 caaaattgca aaagttccat tagaaaactg ctttttaccg caatctcctg caggcccttt
     3601 catataaatt gccttagctt tttttatttc tacaatatat ttcttctggg taatatcccc
     3661 tatatcaaat atctcattgt catttggaaa tccttcaaca ggaatctctt ttctttttcc
     3721 tttaatatta actgcaaaat ccaaaggagt ttcaacattt tttaatttca ctctaggtat
     3781 tttaatgaat atctttctac tcctgagata attttcctgc tcacccaatt ttttaccctt
     3841 atttattaaa caaacttgtc caaacaatcc acaactcaat actttattct ttccaaggag
     3901 tttaatatta tcttcaggtt ttgcccctcc aagaatatac aaagttttac tcaattttaa
     3961 ttttttaaga gcattaacct ctctttccaa tattcttcca gcacaactat ttacaatata
     4021 tttaggaaga agtattgagg cctgagctct atgacaaaca gaaaatgcat cattcacata
     4081 tatctcacaa ttctttgaaa aaaattcagt taatttattg ggatttgaat caaattcctc
     4141 ttttaaattt ctaacatttt ccaaaagtat cgcctctcca tctttcaatt tctttatttc
     4201 tctctcagct cttttcccaa taatatctcc aacaaattta actctaacat acttattcaa
     4261 ttgcttgcaa tgttgtttca aagaaataaa atcctcatct ccgggtcttc cctgatgggc
     4321 taaaacaaca actcttgctt ttttcttttt taattccttt attgttattg atgcttcact
     4381 tattctctca gattttaata acttcttatt ttgaacatca gaatttaaat cagacctcaa
     4441 taaaacaatt ttacctttaa aatcaaaatc atttagagtc ttcatatttt acttaattta
     4501 cctaaattta ttttttactt caaaagctta atcatttcaa ctaatctatt ggaatatccc
     4561 cactcattat cataccacga aagtactttc acaaaattcc cattagtttg agttaaaaga
     4621 gaatcaacta tagaagaatt tggattcttt attatgtcag aagaaacaag ttcatcttct
     4681 gaatattgta aaactccttt catttgtttt tcagcagcat tcttcaatgc tgcattaact
     4741 tcttctcttg tgactgctct tccaacaact gctacaaaat cagttatact tccgcatgga
     4801 acaggtgctc ttatagccaa tccatctaat tttcctctca attcaggtat aacctctcca
     4861 actgaacttg aagcaccaga acttgtagga actaaattta ttgctcctgc tcttcctctt
     4921 ctaaatttct tatggggaat atctaatatc tcctgatcat ttgtatatgc atgaactgta
     4981 gtcatatatc cggattgaat cttaaaatta tcattaagaa ctttaactac aggagctaaa
     5041 caatttgttg tgcaagaagc catcgaaata atcttgtgtt cttttttcaa ttctttttca
     5101 tttactccca aaacaactgt tatatctggt tctttagctg gagcagaaat taaaactttc
     5161 tttgcacctg cccaaagatg cttcattgcc ccttccctat ctgtaaacgc accagtacat
     5221 tcaacaacaa tatctactcc cagttctgcc caaggaaggt tttcaggttg tttctcagag
     5281 aaaaccatta ttttctttct atttaatttt ataaaaccat ttcctttttc aataatcccc
     5341 ttataatttc cataaacaga atcatatttc ataagataca caatagaatc tatactagct
     5401 aaatcattaa cagcaacgac attaattcct ttatctaaag caattttcaa aaaagacctt
     5461 ccaattcttc ctaaaccatt aattgctact tttaccataa tcactccttt ttttatataa
     5521 aaaagaatta aattggttta ataaatctta ctaattaaaa ataaaaaaat ataaaaaata
     5581 aaaaaaagag aatcactttc tctttttctt aactggtttt ttcttaacaa cctttttctt
     5641 agctactttt ttcttaaccg gttttttctt agccgctttt ttcttagcca ttttatctcc
     5701 tctattttaa ttaatctttc tagacattaa aactttataa gtgttttgta aattaattta
     5761 aaattaaaat tactcgtcga caaaaataaa ttaaatttct tggaaattta acttttcgct
     5821 gattaaatct ctcaatatct cttttaattt ttcaatctta actctttttt gaactcttga
     5881 atttctatct ctaatcgtta catctttatt tttcaaagaa tcatcgtcta tagttataca
     5941 aaacggtgtc ccgatttcat cattcctagc ataccttctt ccaatacttc ctgatctatc
     6001 atatgcaata ttaaattctt ctcttaaatc cttaacaata tcgtctgcaa ttttttcaaa
     6061 atcccctctc ttaactaaag ggaatacagc agctttaaca ggagcgattc tcgcaggtaa
     6121 attcaaaact atatttcctc ttccatcgtc agaatatgct ttagttaata tagctaaaaa
     6181 tactctctca acaccaaatg taggttcaat aactctggga ataattttat ttttatattt
     6241 ctcgtcgaaa acttccatat tttctccact ctcttttgaa tgttgagtta aatcaaattg
     6301 tcctctatta gcattccctc ccaactctac atttccaaaa ggatattcat aatcaatatc
     6361 aaatgttgca gaagaataat gacttaattc atcttttgtg tgctctctta tttttatttc
     6421 aaataaattc aaagaatgat accacttaat ttgctcagct aaccaataag cttgccattc
     6481 ttccattctc ttttcactca acatctgtcc aatagttgtt tccttcaaat cttctttacc
     6541 tgcagtctga gtttcagcat ctaaaagttt caacttaatt cccaaatgat tctcctctaa
     6601 atcattacac aatttctctt caggattgag gaaaaattca aattcagcaa tattaaattc
     6661 tctgcttcta aataaaaaat ctcttggagc aatctcattt ctaaaacatc ttcctatctg
     6721 agcaatacca aaaggcaatt gcattcttga agtctgactt attaatttaa aatccaaaaa
     6781 cattccctga gcagtctctc ctcttaaata tgtatcagaa gaatccaaag cgccaacttt
     6841 agttttaaac atcaaactaa attcacccct aatatcaaat tctcctccac acttacaatt
     6901 aacctttcca acttcactct tatctatttt agttgcggtc ttgcattttt tacatacaac
     6961 tgcaacatca ttaaaattag caatatgccc tgaagctttc catgtcttag ggtgagagat
     7021 tatacttgct tccattccaa ccatattatc tcttttattt acaaaaaaat tccaccaatc
     7081 tcttttaata ttattaaaca attcaacacc taaaggccca tagtcccaaa aaccagaata
     7141 ccctccgtaa atatcacttg acctgaaaac aaatcctttc tccttacaaa attttgcaag
     7201 ctcatctatt gaaatctttt tttgaataag attattctcc atcttaatat ttgatttatc
     7261 ttccatttta atctcttgat tatttgaatc catattttta attatttctg catacttttt
     7321 atctgcttct tctttattgc tgcctaaata tgcaatatgc tggcttgtaa ctttatttcc
     7381 ttttcttaca cttttcctta aataaaaata ttctttacca ttaatctttt gtttaacaac
     7441 ataagccatc taaaattaac aactacaaaa cactttataa gttttattaa caactactaa
     7501 aaaatccaat aatcaagcac ggggtgggaa tcgaacccac gaaaattcgc tctgcaggcg
     7561 aacgcagtac cattgtgcca cccgtgcaaa caagtgcccg agacaggatt tgaacctgcg
     7621 acctactact tagaaggcag tcgctctatc cagactgagc tacccgggca ggataactta
     7681 gctactaata atccattcat ccaactattt aaaaggtttt gttttaaaga caaacataaa
     7741 aaatcaaatc tcaaaagaac tgaaatcttc tgcaataaca acattcttga actctttttt
     7801 tgcatcctca aaaagttcat taagtttatt atcgtatctt tgagaaatat gagttaaaac
     7861 caacttttta actttagcag atttagctat cttcccatcc tgacttgcag tcaaatgcat
     7921 atgctcttta gcttctttag aattttttga actaaaactt gcgtcacaaa tcaacaaatt
     7981 agcatctttt acaaatttca caatcttatt attaaaggcg gtatctaaaa caaaagaaat
     8041 tttctttcct ccttcaatat aaatcaaatc ctttgcctta tactttttac cctcataaac
     8101 aatatctttt ccttgtttaa gattttttaa taacggccct tcaggaattt tatatttttt
     8161 tagtttagat ttgtctatcc tatgtttaga tttaagcaca aaattatatg catttgtagg
     8221 tattccatgt tgcatactct cagcttctaa ataaaattct tcattctcat aaaacttgcc
     8281 tataacttct tccaccttta ttttaaatcc attttttaaa tgctttaaat caatgaaatc
     8341 ttctattatt tttttagttc ctttaggaca atatattttc aaatgcttat tatatccact
     8401 aaaattaagt gtagaaatta aaccaggtaa accaaaagta tggtctccat gaatatgagt
     8461 tattaaaata gcagttaatt tacaagggtt caattgagcc ttcctaaact gtctctgggt
     8521 tccttctcca caatctataa gtatattttc atctttgtat gttagtaata ttgaggtatg
     8581 atttcttttt gcactcggta tctgtgcaga tgttcctaaa aaaattactt ttatctttga
     8641 tgccatcaac atatatataa aaaacacttt atataattat caatctaaca aaaaaagctc
     8701 acgctaaatt taatacgccg agaaaacgcc cagcgaaaaa taaatcaacg cttaatttga
     8761 agttaaaggt ttaatccttt tttagtgcag ttgaagcagg agaaatatag atgattgtat
     8821 ctcctctaag gaaagtcaat cccaaactct tcttaacttc tccatcttgc atttctttca
     8881 cattatctaa aactagatta atgtggatat caaaggccaa taaagtgcct acaaaacact
     8941 tttcaccttt caaatgtact aaaacttctt tacctttaga tgtattcaat aaatccaaag
     9001 gtctttcaat tccattagcc ataatcaaaa tataataaaa tcgtttttaa acttttctta
     9061 tatttttaac aataaattct cataaaaaga tttataaata aaaaatcaac ctatcatagt
     9121 atgaaactcg gaagaaaaat cactggtgga agatatataa aaagtatgaa aaagaaaaaa
     9181 cattctttac caggacagaa aaaaacagta aagttagctg cagatgaaaa aagaaaaaac
     9241 aaaagaacaa gaggtggaaa tagaaaaaat tatctcttaa agggaaaatt tgtaaatatt
     9301 caagaaaaag gtaaaaaatc aaaaaaagca gaaataaaaa atgttcttga aactccttca
     9361 aataaattct tagctagaca aaatattctc acaaagggaa caatagttca aacagattta
     9421 ggaaaagtaa aaataacaaa tcgtccttct caagaagcta tgattaatgg tattctaatt
     9481 gaataaaact ctacatctta ataagaaaca cttaaaaacc caaatagaat ctccttataa
     9541 aatatacgaa agctttataa atagaaaaac tttataaacc ctattattaa acctggaaaa
     9601 tagtaaaaga ttatggctgg aataagaaag tgtcctgaat gcgaaagcat caacctaaca
     9661 tatgattctc atcttggaga aataatttgt aatgattgtg gattagttgt tgaagagaaa
     9721 atggtagata ctggaataga tctatcaggt aaatttgaca aaagcgaaaa gaaaggaaga
     9781 ggtggagcac ctatctccat acaaaaattt gataaaggac ttacaacaaa tgtaggagaa
     9841 atatctgata tatacagact tgaaccaaaa caaaccagaa aattcttaag attaaaaaaa
     9901 tggcaggaaa gggtctcaac ttcaattgaa agaaatttaa gacttgctat ggctgaatta
     9961 agagttattg catcttactt aaatcttcca aatgttgtaa gagatgaagc tgcaagaatt
    10021 tataactatg ttcttcaaag aggattagta agaggacgtt caatggaatc tgtaattgca
    10081 gcttgtcttt atgctgcatg tcgttcttat aatattccaa gaacacttga tgaaatggca
    10141 acagcttcag atgttgaaag aaaagaaata ggaagaacct atagatttat tataagaaaa
    10201 cttggaatca aggttaatca atcctctcca aaagactatg tttcaagatt ctcttctgta
    10261 cttaaactaa gtcctaaaac acaaaatgat gctcttaaag ttcttaaaca agcagaagaa
    10321 gttgaattga catcaggaag aggtcctgct ggaattgcgg ctgcagcact ttacgttgct
    10381 gctttaatga atgatgaaaa gaaaacacaa agagaagttg cagacattgc aggaataact
    10441 gaagttacaa taagaaatag atataaagaa ttaattgaaa aactcaaact tgaggataaa
    10501 ataaaacaaa aagaagctga ttctaaaaag aaaaaaacaa cagggtaaat taatcaaaca
    10561 actatttatt tactagcccc tacaattatc taaacctttt tatacaatca atttctaatt
    10621 tttcaatgcg ggggtgccgg agcggtcaaa cgggcgagat ttaggctctc gtagcttatg
    10681 cttacctagg ttcgaatcct agccctcgca cttgtaatta ataaaaaatt aggtaaaaaa
    10741 taagagttaa aaattagatt ggaacaggat tccaaactct atatattgga tgtctatcaa
    10801 ctcttaaatc atattgtgta tattgataaa ttttacatct tggagcatac caatacaaat
    10861 ccttttcagt acaaacagtt ccaactctat ctttttgatt cttaataagg gtcttaccat
    10921 tccaatctga aacatttgct ataatatatt catttccttt aacccaatta ataaatacaa
    10981 cagaatgagg actatcctcg ttataaacta tatctattct gtctccagga acaagacttt
    11041 tcaaaagatc agaataagtt aaagtatcgc ttgaataagt acaactatct gcagttaccc
    11101 aaataatcct gcccgcatta tctttttgag aaccaattcc aacttttgct gtggttccat
    11161 ctctcaaaaa tatagaatat tctgttcctt tcttagtcga ataaatacat ctagataaac
    11221 ctgcagaagc atgttgataa atataattag ccgcaactcc acaattatta ttgtatacct
    11281 ctggaacaat agttccatcc aattcttgag cagatgtcaa gaccttctgt cttattgggc
    11341 ttaaagtcga aggagtggtt ggagaagtag gagttgaagg tgttgttggt gtagttggtg
    11401 tggtaggggt tgaaggtgtt gttggagttg ttggtgcggt acttgatttc tcctcacatt
    11461 ttccataacc taaaacaaga tttgtatgaa cacaattctt atttaattta agagcaatag
    11521 ctttacattc atctctatca cataaattag aaagaccatc tccacattct tcgcaatttt
    11581 taacagccaa aaatagtttt ctctcagcag ttgaaaacat tgaaagttga acatccttgt
    11641 aagatgtgca aggaggatta atcttttcat aatcacaatc tgttggagga attgaaggta
    11701 ttcttatttc atcgccatct aaagttatta atccaaattc ttttaatatc tttaatccct
    11761 cttcgtaatt ctttccattc aatttaagaa ttaattcctg atttttatca ttaagagttt
    11821 ttccgtcata acttccccct ttaatagtat aagaaggtac agattcccaa gaagaatctg
    11881 aaattgattt agtataaaca ttccattgcc aaacagtatt atacctataa aatactacgc
    11941 tactgtgtgg attaatagga gaaaacatca tatgtttgta aacatcagaa gttgaagggg
    12001 ttgaaggtgt tgtaggggta gttggagttg aaggagttga aggagtggtt ggtgtggtag
    12061 gagttgtagg agttgttggt gtacctccgt tatataatct aactaattca aaatattttt
    12121 ctttcacatt cctaacataa tgaatatctc ctttgctaca atcagtattc cacccattat
    12181 aacctcttaa agcatattcc catccaaaat aggtctctac aggcattaca caagtgtaaa
    12241 ccctgcttga aggactataa gcttctttta atatctgagc accaatctta atattttttt
    12301 cattatcatt taataatgta gaaatacaat ttccagaatt actgctaaga ccttttgtgc
    12361 cacaatgaag aacattaatt tgcatcaaac ctatatctcc cccatcagaa gatttaactg
    12421 ctttacatga agattcttgt atcataattg ctaataaaag aagttcatct ggaatattgt
    12481 tagaattagc tgcttcaaaa atccattttg cataattttc acattcttta ccgcaataac
    12541 acttaaaggt agaagcataa acagatgaag aaagatcttt cattaattta gtcaccttat
    12601 ctaaatctct tttttgaaga ttagcttcca aagaagttgc aacagaggta gttgaagaac
    12661 cacctacaaa atatctttct tgaatatctt ttgaagttgg attaaataat attgctgctc
    12721 cctcataaaa tgtctttcct tctaatgcaa gtacaatagc tttttgtaga ttattaaatc
    12781 caggagcaac atttgaaaca ctatgtcgaa cccctcctat ctcaaacatc ttatctaatt
    12841 cccaatacca ctctccatct tcatatttat aataaatttt tttcataggt tcataattga
    12901 catccttaaa tttaacagtg aataatcctt cttcgtccat actagaaact ttaaatgttt
    12961 tttctgaaag ctttgcattt ctatcttcca tagttttatc tataagaacc tttaatcctc
    13021 caagataatc tccattgtaa ttttgaagag aagtaataat ttctttactt cttttagaaa
    13081 cagggtttgt atactcatta cctaaaacat ctacattaaa ccaagcaact gtaggatcac
    13141 atgaaattgc aaattgccat ttggaattaa agtatctata gcacctatca tctagtgtaa
    13201 gaatattttt atcactagga agaagatgtg gtgaagtata ttttatattg aaccacgaat
    13261 ctcctccaga tgttgtagtt gaatcttcaa cataaccaca ttttccatta acttgccctt
    13321 tgtttaccca tttattatct tcacaaacaa agtgtgttgt tccttcacac ttagtatttc
    13381 cattagttgc gcatcttcca aaattattca ttgctaattg ggcataagat tttcctctcc
    13441 agaaaaataa atatgcttta tgattattaa agaagatttt gtcaatttca aaaatagaat
    13501 caattaaatc tattctcttt aatgggtcat cttcactttg aatatctttt ttcttggctt
    13561 caaattcttt ttgagtaaaa taatcttcag aaagataaga taaagtttca tctttaatac
    13621 tttcaattgc atcttcttct aaattaaggt ttttgattgc ttttctaaca ctctcagtat
    13681 caatccaaca tccaactctc tcatcgccac aatgtccaac tttttgccat cttaaatctt
    13741 ctgaatcaga agacgaaatt tctggtccta gtcctggatt atgagtagag caaactcttg
    13801 ttataccctg agcatataaa tcaggattaa ttaaattttc agctgcactc aaaacattta
    13861 aatctaaaag actgtaaatg ctagctgttc cagacctaca atcctcttct gaatcaatat
    13921 tcttcttttt aacttggtcc ttcaaaaaca aatcacttaa ataattaaca gaaaaagaag
    13981 ttgaaacttc cttgaaccca cattcctcta caccattaac ctcaatacac aattccctat
    14041 aacctgcagg agcagtaaag tcaatagtag cgtcagcata tcctccttta gggatataac
    14101 ttgaattaac aactctcctc tgaacattat ccatataata attgcttgca gaacctctaa
    14161 gataaactct gtaataagct ccagaatctt ttccagcata aatatgatac caaaccttgt
    14221 aatgagaagt aggaggattt gtagatgttg ttaaaggtat ctcttcaaat ttacctgtaa
    14281 attgtgaggg agaatcagga gcaattaaat tactaataaa acttccagaa tctgcataaa
    14341 ctgcagaaaa aaatctccca caagcttctg tgcgtacagc atccattgaa cgaacttgga
    14401 aagctctata agactcttta gcataataat tcttaagata gtctacagta ttttgagaat
    14461 tttttaaagc agaagcaaat cctttatact ctccaccacc ttgagatttt tgtcctaaaa
    14521 ttctctccaa taaatgagaa ccaaagtatt ttattatagg agttgcctgc tcccaaaaga
    14581 aactacaaag ataaatacta tgtatttcat cacaaatacc gacagtttct cctgtctcta
    14641 agcttttttc aagacaatct gaatatgctt tttgaacaga aataagatta tctatacctg
    14701 cttgaactcc tgttaaacaa acaggaatat aaacatctcc tccagtaact ttccaattag
    14761 gtaaacacaa aaatatactt ccaattatcc cagaactaat aacattctta acaggatatt
    14821 ttcctccaag atcgcatctt gagcttggac aaataacagg gtcacacaaa ttaaacaaaa
    14881 gattacaatc tttaacagac ataaaatcag tacacctaaa tccaaaatct tcagaaggag
    14941 gctctccaat attcaaagaa ttaccattta tatttacaga acttccagga gatattcctc
    15001 tcatacatgt ttgctgtgct tgtgctattg acctaacagc taaatcaaaa taatcttgaa
    15061 ttgatttccc ctctaattga ctaaattgag aactagataa tccagcagca gttaaaagtg
    15121 tgcaaaaatc atctccaaat ccactaactg aaaattcttc tatgccatta tctccaacat
    15181 tacatagata aaacatctca atccttcctg aatcactata ataaggcatt tcagctgatt
    15241 tcattccagc ataccatcca ttatgagtgt ctaatggaac aagtgcaggc ctacaagaat
    15301 gagaagaact ttcaaagaat ttaattaatg gtcctgtctt agcacttcca taagaagaaa
    15361 gatatctgtt tttatataaa ctgtctcttt taactatagt tacttctctt cttaatttac
    15421 ttaaaatctc tttatctgta aactctgttc catcagtata ataaacttta tctataggat
    15481 aaaccccgct tcctttagat ttttcaagta caaaaacaag atttcctttt tctatagcag
    15541 gtattgttgt tataacgata ggagtatctt taggaattgt atcaggtaaa ttagtttttg
    15601 tgtttccttt tgtatttccc ctataatcat atttaacagc attttcatct ttaagttcta
    15661 tatagacaat atgggaagga ttaactcccc ctaatgtttt tgctgtttcc aatgcattaa
    15721 gatatttatc tgaattaatc ccaaccttag acattgcaga actcaatctt gaagaagctt
    15781 ctatatctcc gctattctta ttcaaataag tagcataaag ctctatttct tttaattcat
    15841 taactgtaaa tgctccctca gaaactaaat aatctgtgcc atatttgctt tctgaagttc
    15901 ccctattatt aagtatactt tgaatcttct gtctgttcaa ttcaatcatc ttctcttcac
    15961 tcattcccat tatttcatct ggacttaaag aatccttatg taaagactca gtcaaagaaa
    16021 tcaaagaatt tctatccatc ttagataaag aactatcaac agaactataa acatgattag
    16081 aatagctaac cataaaaagc ttttcattca attttccatc ttttgtatat gtggcttcta
    16141 attctcttat tttattattt tgttgggaga taatgtttgc catatcttca acttcttttt
    16201 caatctcctc agcattatcc aataaacaat cctcaactga tttatattta tataaaccac
    16261 tattataatc ttcttgacat ttttcattcc atcctcctgt accagccatt actttttctc
    16321 tggctattgc tttaccattt ctattcgcaa tcaaattctt aacaaccaat ccagcacccg
    16381 tagctccaca agcgaatttc attcccctat ttattgaatc taaagtgcta gatatttttg
    16441 ttaaatcatc tattcttttt tctaattctc ttaatttctc ttttgtttta tctggtgcaa
    16501 gttttattgc tcttttttca attcctattt taaatttaaa actagaatta cttatagctc
    16561 tctcaatatt tgaatcaata gaaactttag caacacttgc aagagttgta tctacaagtg
    16621 taaaagaata atcccctata tctggcctct gaacatttaa attcaatttt tcagattttg
    16681 tagaaacagt aatctcttta tctcctttat tatttgtagt tgttttgcca acagcaaaag
    16741 ttatttgtgc agaacctgta tctaaagatt ctaataaaat atattctcca gaatctaatt
    16801 tctgtgtatc ttcctctgtt ctaaatccaa ttaaaggaat ttgtttattc tttcttaaat
    16861 tatatctctc tgttctacca ttaggacctt taacaactac ttctgcatta tattcttcta
    16921 aacttggctc tttaactccc aaaaaagaaa ttctattagt aatatcattt atagttactt
    16981 caactattga agctttattg tttgaaagta aaatattgct atcacatatt ccaattcttg
    17041 cttgagagtt aggatattct tcattaaatt ttttacataa ttccaaaact gttctcattt
    17101 ctttaacaga ttcagccaaa taaatttcct ctattaatgc tctctctcca aaatcttgta
    17161 tatcactttc atactctctc tcatttgaat aactatctat aactttttca aaatcctcta
    17221 tagcagaatc ataataactt tgataaagtt cttcatcact ctgaactaca tcaaggtaat
    17281 ttcctactgc aaatcctcta agtgcaactt ttttgtctgc aaattcaata ccttcagaaa
    17341 ttgctccagt ttttttattt ttatatggaa caactttaaa agattctcct gtaatagccc
    17401 acttaaagaa acggactgta tttaccccaa gattacttaa aaacccgtcc ttggcttcag
    17461 cataacttgc aataaaataa agctcttctt catccaaaga atttttcttg atattttcag
    17521 aaataggtaa agaaacaagt ataacatata aatcatttaa atcctcttca tttccatcac
    17581 ttcctatata tcctaaataa actttttgat actgtccttt agaatcagta tatccatata
    17641 aaaaatctcc aatactatat tctttaggat ttcctccatc taaactcaaa ttaacttgtg
    17701 gagataaact taaagtataa actttttgtt tcttctcttt acatcttatt ttaacttgag
    17761 agcttaaacc agttttaaaa atatctataa ccgtacatct ttcatctaga aatttcccat
    17821 cttttcttac ttccacataa tctccattta ctttaaacat cacagcagta tcaggacgct
    17881 ctattccatg taaagttaat tgtaaatttc ctaaacaagg atttattcca ggcaaaaata
    17941 cctttcttga agaatctcca ataccaagtt taatgtttga ggcatatctt gccaatctat
    18001 aatcaaatcc tccttttcca gaaggtcttg caacagaact agcagaataa atagaaagaa
    18061 tcgcactgtc ctctgtaact gattctgctc tcaaataccc tctaccatcc caaaaaccat
    18121 aatatagata attatcttcc cattctttat cattcataac aggaagataa aattctgcat
    18181 tacctaatcc aaatgaattt ctaacatcat accttagtgt agccgtgata ttcccttcaa
    18241 cccaacaaac ctcagacaat ccaaaagctg ctttttcaca attagttaaa gcagattcat
    18301 taggttgtct tctcaatgta atcacaacat atccaaaatt attattaata agtgaaccgt
    18361 ctaaattatc ttcagtatat cctaatgctg cttgagcagg ttgataacca atattcaaaa
    18421 cttctctagg atattctcta tcaaatctaa tgccatctac atctgtaact tcaataaatg
    18481 gattaatttt tattgcagta agtttacaga atacattaac attttcttct tctaataaat
    18541 cgctcctaac aggagaagga atacattcta aaggatttat ttgtataata aaatctgttc
    18601 cctgttgaca catagattca tctattctaa ggctctctcc agtagaaaaa actccctgtc
    18661 ctatagccct attatactta ctggaataag taagagattc agtatatact gcacttatca
    18721 aaggaacaaa taacaatatc ccaaataaaa agaaaattaa aatcttaaat gattgattta
    18781 tttttttcat cttaagttta ttttcaactc cttgttttca aataaattat aattcttttg
    18841 aatttgttca agagtatttg gttctggaag agtctctgca taaatatcaa taactcttga
    18901 acctctcaat tgattctcag agaaagtata attgagtttt cctcctgctg aaagagcatt
    18961 tgaaagagtc tgtgcaggaa cttgaacatc aaaacaaact tcttttgtca aaccaattaa
    19021 tcctcctatt gatgaacgag gaacttcaac acactgcttt tgagtcatat tccccacctt
    19081 aagattagaa ttcttatata cataaacttg cacctcatat tctccctctg ccaattcaac
    19141 atacttttgt cttgggtaat atatcgtctt tgaaaaatca tctgaaacaa aataaattat
    19201 cgcatcacga ttataatttt gattatttaa tttaagttga acactaagat tatacaactt
    19261 acttaaataa acactcatta ctccctcctt aactgtagaa tacataatac tttctggctt
    19321 atatccctct gatttaacat tgataaatcc attaatacat tgagggaatt tttcagaaat
    19381 tctcccgttt ttagttgttc cgatgctaca agtagaaccc aagcaagaat aagaaacata
    19441 agcttcaata ggtttcaaat ttgtatcata cacctgtata ttcatgtaag tatttttatt
    19501 tgtacataat tcttgagaat caattaaagg aactgattct ccagaagcat ttctcggtag
    19561 attcctctca attaccactg ccataggaaa ttgaaagatt tcttctgaac cttttcctga
    19621 aataatctga attaatacag gatatctaag actataaaca aaatgataag gaacataaca
    19681 aaaacctaaa atatctaatc ctggttgatt tccaacagga tttgcagtca taacaggacc
    19741 atcagaagga tttacttcaa agctataagt ccaatcttta gaattcaaaa atctaactct
    19801 gtgatttgaa ggaatatctg aaatctcaac ttcaaaataa tcttctttat tgtttgaatt
    19861 ttttaaggcc aaagtatttg cttctattgc ttctctcagg tctctaaata tttcattagc
    19921 tatccacacc ttaggagaac aagttaactc aaacccatca acaggagcat ataatcttaa
    19981 tatatctatt gcataatctt ccaaaaataa actgctttgc tcttcttcat aaactttaat
    20041 tgcagattca taaagagaac caagctcaga atcaagaaca actttatgtt ctctaatcac
    20101 atgattttca ttttctcttg aaattattaa atccatcttc aaatccaata caacttcatg
    20161 gtcttttatt gttatttttg cttttggttt ttccatgtct atccaatatc cttgtgaata
    20221 ataatcatca aaaaaacaag ccctagattt tgtttccata aacatttcca aatttttttc
    20281 catagaatcc tttgatggaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 aagctcctgc aacaattaat attgccaaaa taataaaaat agtgacttgc cctcttttaa
    20461 acctcatgga aaactaatga aaaatatgta tataaagttt attgaaaatc caattatcta
    20521 gctctattat atcgactagc tctattcgct ctaagatagt ttacagcacc aatgattagt
    20581 aaaatccctc cagcaaaaat aatccaccta ttaatatttg cagcaatttc tggtaattta
    20641 atgaagttca atccaaaatt aagaaaatat gctcctataa ctaaatacaa tacaaaaaag
    20701 aacaatcctc ctcttgccat atttaatata aaaaaagtat atttaaatat tttactaatt
    20761 ataaaaaagc atacaaaggt cccatagtcc aatggctaag acatgtcctt gacgtggaca
    20821 agatccaggt ccgattcctg gtgggaccat ttaccattaa ttctctaata aatatagaaa
    20881 atagaaataa tctcaaaata gaaactatct cctacttatt tttccaccct taacctttcc
    20941 ccagttctct tcaattaatt caaatttaaa atcacttata ttaataccac ttattcctat
    21001 tagctcctta aaaagatact ctggaattaa tctttcttca ttatagtaat tcttaagagt
    21061 agagtaattt acatcaattc cccgattaat taattctcta agagaagggc agtttatagt
    21121 ttccatcacc tttttaaaaa attctctttg ttgacctttt gaaaacttaa ctctcatttt
    21181 gtttgatttc agcagaggta atttaaaata aaccaaatga tttcccaaat ataactgcgg
    21241 gaccatagta taaaaaactt tttaaaccaa ctaaaacaaa gacttctatg aaaataccta
    21301 aaacaactaa aagatactgt ccatactgta aaaaaaagac agaacaaaaa ataaaattag
    21361 ttggtacagg atttcaaaga ggtactctta ctagaggttc aaaatacaga gctaaattaa
    21421 gaggattagc gagaggaatt ggaaacaaag gtaaatgggg ttcaaaacct gctgcttcaa
    21481 aattcaaaag aaaatcaaaa acaactaaaa aaacaaactt aatgtatact tgtaaaaaat
    21541 gtaataaatc aaaataccaa aaaaaaggta taagactaag caaacaagta caagaataaa
    21601 aattaaataa gattaaacaa atacaaaaat aaaatggcac aagcaaaaag gaaaaaaaag
    21661 ttttttgatg tagaaatgcc tataatcaat aaacaaactc aaatatttgc atatgaactc
    21721 gcagaactag aaggaaaata cattagatat gatttaacaa gaattctaag aggaaaaagt
    21781 atattgatgc aatttagagt tcaagttaaa gataataaag caatagctat tccaagagga
    21841 gcaatattaa tgccctactt tttaagaaga atgataagga agggaacaga ttatgttgaa
    21901 gattctttta ttgcagagtg taaagacgca tctcttaaaa taaagccatt tttaataaca
    21961 agaagaaagg tttcaaaaag agtcagaaga ggattaagag aaaaggcaaa acaagaaata
    22021 ataaaatatc taaaagacaa atcctctcaa aaaatatttg aagaaattct aaaaaataaa
    22081 atgcaaaaag atttaagcat attattaaaa aagatttatc ctctagcact ttgtgaaata
    22141 aaatcaatag aaattaaaaa agatttagaa gtaaaagtaa aagaaaaacc aaaagaagag
    22201 aagcaggaaa aagaagaaga tattcaagaa aagaaagaga aaaaatcaaa aaagaaagaa
    22261 gaaaaagaag ctgattctaa agaaaactaa ggacaaatcc taaaatacga aatcttttaa
    22321 accaagataa ttacataatc ttatacataa ttaggtataa ataagcctgt atagctcagc
    22381 gataggctca gattacatct ggctaaatcg ctttgttcat gcataaataa gcctgtatag
    22441 ctcagcgata gagcaactga cttgtaatca gtaggtcggg ggtttgaatc cctctacagg
    22501 ctttttattt tttaaactct aataatattt ataaacaaac tatactaatc tatttaatgg
    22561 taaaaaaaga gggaatcaca ctcaacaaaa aagttattca agaagcaaaa aaaagacata
    22621 gggatgcaat tgatacttgg aaaaagaatc tagaagaagc tataattaca agttataatc
    22681 acataaaaaa atatccaaat agttctgaaa aaaaattcga atctgttaaa ttattattaa
    22741 aagatggatt agctctatta gaagacagaa catataaaga aaatttttca gaaggaagag
    22801 aaatattcta ttacatgaaa gatgcgattg ccaaaaaaga aagcaaagca attctatcca
    22861 atgaaataga caaatacaga caagaccttg ctttaagagg aggattatta gctgaaaaag
    22921 ctcatttaaa aatggatttt gttgatggag atgcaaaaat acttcattat tatgacaagt
    22981 actttggaaa taggtctcca attttagaaa aatcaataac aatagtttca ctactttcaa
    23041 tcctgttagg aatttttatt ggatatcctg cattaacagg aaatctaata gcagaaacag
    23101 taaatggttc tcttgcatat ggagcaatac ttttctttgt tggacttctt ggagtattcc
    23161 ttgcaaacaa aagataagaa ttcaaaagtt ttattttaaa aatatcaagc taatttaaca
    23221 gctgttccat aggaaagcat ctcagaagca cccatcatta tcatagcagt cataaatctt
    23281 actccaacaa cagcatctgc tcccaattca attgcctgat ttatcattct attatatgct
    23341 tcttctctag attgagtcat cattgaagta taacttttta cttcacctcc aactaaattt
    23401 tttaatccag ctccaatatc tacaccaaca tttcttgttc tgacagtatt acccttaaca
    23461 acacccaata cttcaacaat ttttctacca ggaatagttt cagttgtgct tacaataatt
    23521 tcctttgcca cattatcttt tagttttaag tttatttaaa tcttgtcttt gttctatttc
    23581 atcttctttt ttattaagaa taattactaa tccaatgatt aaaattggaa ttgaatatat
    23641 cagcataaac aatagagtaa aaaaacaaac aatagtccaa attaacccta tactaaaaat
    23701 aattccccca ataataactc tttcattttt catatagtca aaaaaagaat aatcttaata
    23761 aaacttactt ttcaagaatt tcaactttct ttccttcaga aagtatctta gaaatttctt
    23821 taggtaaatt tgcaatatct cctttcttat aaggacccat tttatcttca tctgcactta
    23881 aaaattcccc cacatcctct aaaaaaacaa ccaaatcatt atttttaaca agctctcctt
    23941 tgcctattcc atttagagat tcattcaatc gtttctctga ataatccata catctcatta
    24001 acccctcaaa aagttctttc tctatagcaa gcatattttc aaaatcctgt tttgagattc
    24061 ctgtttctgc agcaataaga actaagttta gaattttctt tcttcgtctt attataagtt
    24121 ccttaaataa agtcgtagca ttttccaatt gtttttttgt tttaattgct gtatctgaaa
    24181 aatcttcctc ctcttttgaa gcaacttctt ttttctcttt aagatatttt gagaattcag
    24241 aaacaaaatt tttaggtatc tgttgtaatt gctcggaata cctctctttt cttgctgcct
    24301 catagatatc attataagta atcataaaaa caaaaaacat caagtgtttt taataattat
    24361 tgtattttga aaattagtta caagatatag gatacaattt agattatctt ttaaatccca
    24421 gataataaat tctcacctta tgcattctaa catcatctgg gtttcttgaa ctataacttc
    24481 ctcttcttct atgccaataa tcgtctatct gttttaatgt gaaaattttt ccatcaggac
    24541 aaacaacaga attacttggg tcaataactg aaatatcaaa agaagaggca agcataccga
    24601 aaccttcatt ctctaactgt tgacatctat aaaataaact tccttcttta tctgaatcaa
    24661 aaacatcttc tgatttctct gtccaaggaa ttttatttac taaagtttta ttgttatttt
    24721 ccatacaaaa taattataaa tcaattactc cctctttggt caaagcagca aatctgatac
    24781 caacaggcag attaatcaaa gcagaaatca aagccatatg ctcttttcct tctccacttg
    24841 caatagaaag tgcaacttct gttccatcga tttttccttt taattttttc aaaaattcat
    24901 ctctcaaatc ttttatcttt
//
LOCUS       DAOPCH010000004        24754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_2174_length_24754_cov_6.712053, whole genome shotgun sequence.
ACCESSION   DAOPCH010000004 DAOPCH010000000
VERSION     DAOPCH010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 24754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24754
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2174_length_24754_cov_6.712053"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..947)
                     /locus_tag="PLU55_00520"
     CDS             complement(144..947)
                     /locus_tag="PLU55_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86576.1"
                     /translation="MSERNLNFWLTDKEFISKSKYNDLYYLEGLCYRIASFLKNTPIH
                     VITILDNTEMSIGFSIGTNKKYYPISNDISFYDYITEVEKWLKTFYPSFDYMEELEVE
                     LSEEEMKTKIKEGMNLNDVLLLRKKSYVRHYGVVEKIYLKDDCFLLNYNGNLEKRTSI
                     CTTNAVPVSKFMEKLRTLSEKERYEYIKNDSVKLHDITEKQIEIDYAGKTMLNFFEIN
                     YDVLKEKSLIKVSEFVYTWGRFVINFNSPLLRDDCIKLYNKLLIGKNND"
     gene            complement(976..2043)
                     /locus_tag="PLU55_00525"
     CDS             complement(976..2043)
                     /locus_tag="PLU55_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86577.1"
                     /translation="MKVLKSELNFIDWYSKFANNKNLMNDKIQFIGKAETKEVFVIND
                     TGTSILYTLISKDIEKDFISVYPVNEFTSILSGIKDDVEIELADNTIKFGKSKYEFPL
                     FDVIPSEFNHYREKIENEEVAFTLDNISMFPELGNFVGAVEDNLNGVIHYSGHYIASN
                     QKKATAIFNRNSENVIPEGFSVAYDVFTFLSSLKIESIGIKEFEDFYSFKVNNTVFVL
                     KKSKNIRLPNLLEPDKKARYNNEFSIEIDKAEFKNILNRIKIISKDNIDTRLFFTCKE
                     NELVIESKDGKYAVEKLNVSYSTELEEFSFILSTEWVLKIINCITSKSLKMMCSLENK
                     VINFIGDDTNKLYIHNIFRRD"
     gene            complement(2061..4124)
                     /locus_tag="PLU55_00530"
     CDS             complement(2061..4124)
                     /locus_tag="PLU55_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPJ86578.1"
                     /translation="MKINILEIKNFKAIKHILFDNNSKVNLITGENGNGKSSILDAIR
                     YILTDELDDKISSFVRWGTEKFEILCVFEHKGIEYTLEIEGSKGAKKRLTFNDEEYFN
                     SEATSKLRDIIDFELTKFSAISEQGKTAQLLFQKKTERLNTLSRILKIDSINESSENV
                     KEEVRKIRENVKVLEAELISLENAEFSLMDEIALPDIDEVKKEFEVIEKEKEEYNKKQ
                     KELDKEISEIKENISEIKLNFEKKKNESISNIKTEIINLENKLKEIKRKKNEFDSAVI
                     SSKTIQDEIKDIDNSVEEIAKKIKEVINPSQSEEYVQQIYTKKIEVEQNIKSIEKEIK
                     SLKDRKCPYTFDCEKLEGVDVSKYEKDLESKNTELVELDTNYKQLSEEIKEYKETLKF
                     NSQIEKQILDADIKISLKKESIKKYQEVIDNYVEEDYSDTESIIKLKKEEIESIEHLL
                     TIERDKQIEVLQERINNLLEREAFSKEKKYTDLKNKILLYDNQVKELERIKLFNDEVL
                     KKKSKNERIVSVKKRELESKRKEINLMEEVRDAYKDFSAYRINKGAEHIKNRMNSIFQ
                     KGYGKYEITFEQDKSGIDFFYKEVGSDILNNVVMASGQEKAVLAVANRLALCSLQNLG
                     ILVLDEVDNNASDENSVKLFQTILNEKGFNQIWIISHCNATKEYLNNLVETTNYNIVD
                     GQVEN"
     gene            complement(4191..5348)
                     /gene="recA"
                     /locus_tag="PLU55_00535"
     CDS             complement(4191..5348)
                     /gene="recA"
                     /locus_tag="PLU55_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPJ86579.1"
                     /translation="MGDLKGLMNEASKKFGEGSLIQGSNCTVSPIICSTGSILLDYAL
                     GCGGIPEGKIIGITGMPSGGKSLLCLHMVREAQEAGYNCGYIDAENSYDPIWSEKMGV
                     DNEKLLISQPNSGDEAFALADMMAKSGDIKLIIVDSVAALTPKAVLEGEYDQNHIGQL
                     ARLMSQSLNKINSILVKNKTTLVLINQQRMKIGGYGNPITEPGGEAIKFYASVKLEVN
                     RSEVIGDKEDVDGFLTRIKVVKNKCGVPFRKVETNIYLGKNGKYGIDKNEEIFTLAID
                     NAIVKKAGSWFSYGDERLGQGKDNALKTVIDNPKWLEEITNAVYDSVLKITKPKENSF
                     NAKIKEAISTDTKTTKRRSKKEEGIIGVEEISTPVEVKTEEVIEAETVEEN"
     gene            complement(5336..5887)
                     /locus_tag="PLU55_00540"
     CDS             complement(5336..5887)
                     /locus_tag="PLU55_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86580.1"
                     /translation="MIEFEDFIKKCESGFCLRQTIKSKRCITKGKQEDCYKKYILKSE
                     KDVEKRKQKEVTPKWEELKREILIRDKNECRFYAIATKEEREVIDKMILENPKLAVRD
                     GAHVLARSKCPEMIYDTDNVYTLYRGVHSAIDHLIDPITQKPMSYEQSQNYWKRIIGE
                     QKYNELYERYMQIQIRRFYKWEI"
     gene            complement(5935..7344)
                     /locus_tag="PLU55_00545"
     CDS             complement(5935..7344)
                     /locus_tag="PLU55_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86581.1"
                     /translation="MSILQEQLEQRKKQEEQKKRAKENSGNFQGFDELSYCPITKEET
                     VFRIVGKPFPYNDPTNRKPTDIKMIMQSEIVKEDKKGYVKFNFPYHYDDTKGDYVLDK
                     DFILTKVINKVKEGRWEKYPEGKVNELTGKNGEYIHYYKDTEVYKIIVDKVGNAKIGE
                     KFPKNFYPSKRVVINVIDRHDSWCKDNKHTKILTSKHEGFSIKDSKTNQDITIFYTDT
                     GIPKTTYDKIVEYHAKGTGNFDVDTIVKKVEKDYTVADITDACPKFITQKSKDIGLDS
                     DLTDEEKSYELYDFDKYFPYASSATKIKKWLSWLIKKVDAQTGSNFMVELEALAKIEE
                     DERNAKKEAQAEKEQVEGEEVSDDVKEMEAPVKEEPKPEVKQEEPKRRRVEEPKKEET
                     KEEISYVKYFPSYDKLSDEEKASLLNALSEIKDGIPLFNNKEGELLGCKDEKCKFPNS
                     EIDTIFPESIFTCPVCGIR"
     gene            7979..9754
                     /locus_tag="PLU55_00550"
     CDS             7979..9754
                     /locus_tag="PLU55_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86582.1"
                     /translation="MPVSKHHNYVKNKYPEIIKFLESLGYNLKNNFIFQKTKKVRNHV
                     NTSWTENKTTPFDNNLIVLENDLYNGKTKEYYKKNIYVSKISLIINHIFSDILSTDYH
                     YSVIDNILESELAGKNINKINKNVGCYFKEKSNVLVIDIDNHDNKNTLLMLETYQSTL
                     DFFGKDNIIFQENSYSYGYHLFIKLNTALTISEKKKLIKDYKKSLHKYSKFVESPLKM
                     RLPISYDYTVLDNKYSTSADYNNNSQNCIQETILAYDTSKGFECESYSVNTTKEKAIN
                     EIYCRETINNSLSNMSYEQFLQDEKFDLYSGERFCKMPFFVSFAKSHNLSEEECFDLV
                     ILKGKGSKDVLSNHKTIKSEIKSLYKNYNSQSIKYDVNKVQEYFSNIDKIPLAVVNKV
                     THKKVLIQILVNTKQKVSNHNMEILKILLLEILGKFFYNLHNRKAIKSNNYTDKFLIG
                     EQYSRNMCNLLKEKYEILKNVNVYKRISDILLYSCLFSQQFSNKKGYYCDYENTGKSF
                     CRQFYLKANKDKEIKFDIDRLILFINESIDYLFNKIKTFSIVNWKECIFNSIKEINSI
                     VHIFSEGSTVNHNIMNDFQFQKMKT"
     gene            9824..10387
                     /locus_tag="PLU55_00555"
     CDS             9824..10387
                     /locus_tag="PLU55_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86583.1"
                     /translation="MGKAKKEKAKANFERKSKRDSGKTIKTLGDYYWSVAKEELAKEV
                     PYMMFENYMKKGFIGNYILNFAKKKFNLNTEKEWMSIKTISSVLFLHALEFDVEKDMF
                     TFNAESEYQGFIDYLESTIVVINEHAEKAKEMSKEDEEKLSDFVKFHTRSDLIYDKAV
                     FSYFLENFKNNTIKKEFIKEEVKEDKE"
     gene            10387..10881
                     /locus_tag="PLU55_00560"
     CDS             10387..10881
                     /locus_tag="PLU55_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86584.1"
                     /translation="MGREVAKEIFHRYRLTTDYKENFICLNRENLSKDFIDFHNERKD
                     FENYIRTKKNPEGILDYVKLDRISNNKEYDSFYYYVKEPEKYYSYQELCNMNRKELET
                     ISYIYGLPTIMVVNSIMIKNIMKEQEEWFSDEATKLREESKKDSNSFEDAFKDIVKQA
                     ESQA"
     gene            10910..11116
                     /locus_tag="PLU55_00565"
     CDS             10910..11116
                     /locus_tag="PLU55_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86585.1"
                     /translation="MVIEGTLKIKFTVKDGALALSDLSEMNKRLEGIIEQSASEIEEV
                     CDYLGCKIISNERPITIDEKLCIV"
     gene            11128..11484
                     /locus_tag="PLU55_00570"
     CDS             11128..11484
                     /locus_tag="PLU55_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86586.1"
                     /translation="MKYKLLSAKSGKSEQEIKEVHKQAIQEAKILGLKDNLLEEFCLT
                     YIEEEFDLDNIKKVTTNLNRAFEKSNHVCFKDFMDDLFVEEGEASSATMSTDFPVEDR
                     PQGFKKKKDEEEKVNV"
     gene            11477..11893
                     /locus_tag="PLU55_00575"
     CDS             11477..11893
                     /locus_tag="PLU55_00575"
                     /inference="COORDINATES: protein motif:HMM:NF017666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HPJ86587.1"
                     /translation="MSKNFNTNTDNHDEWLTPKYITDFFEFDLDPCSPIQRPWDTAKN
                     HYNKIDDGFSKEWEGFVFCNPPYGRETYRWLDKCAEHGNAIGLTFARTETIGFHNTVW
                     DKADALFFFKGRLAFHYVDGRKGSSANAPLSINSLW"
     gene            11980..13026
                     /locus_tag="PLU55_00580"
     CDS             11980..13026
                     /locus_tag="PLU55_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPJ86588.1"
                     /translation="MASKQKRYYDEVTIGEYIPYVYLAIVERLQIYFCGLFYPDEDFE
                     ESKKRFILADFVGGGNETAIRNSIEAYKTSQGKFPFTAYNIDDDEPVEYKSLTHLTGQ
                     FYDKELDCYIASVPAYLNISMMTVYTTEYDYFIGTSKLMYDLAALTRLEVPIVVNNKE
                     TFFEIDIDMDVTKGDLAFAYQEQKDKGSLFNISHNCKIKFPYLILSQRGFVSDDGKQK
                     PVPVYPVDNMILSLKNMKDKSVIDVLNVNVIPEVLSSTPSNNATNVNRTSAIEINFNT
                     SMNEASVINSMDIVPEIWGTKEWNDSGTTLAITPYDSMNANTEYNIFISKQAQNFLTH
                     PLKSDFELKFSTGS"
     gene            13028..13789
                     /locus_tag="PLU55_00585"
     CDS             13028..13789
                     /locus_tag="PLU55_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86589.1"
                     /translation="MYKDVFTEKTRINPKAISLKLQGYTCSHPVLTEAVIRPNNYAKM
                     LTNYFDYVCKRNKNKLEKGLPFFLFELNKRFREYNVIFEIENIGSPYNQRGIHVMSTS
                     TSTGKTIVKCNYKLLESLENKESYNRLLHDFFVLIGHELIHRGQFYYRKYKELLSYAY
                     YNETNYFEDKDEIMAYAWCIVEELRFGGYNNADILKMVSESKKFTVIKKYSDVFKNIE
                     EQLYHKNKKLFNLFLKYLYEYIKGNHFKNQIIGLK"
     gene            13791..14345
                     /locus_tag="PLU55_00590"
     CDS             13791..14345
                     /locus_tag="PLU55_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86590.1"
                     /translation="MQYSVVDKLSQKISPVIRKLSDINIKRLLGTETQLLRIVTSEPN
                     VMGEYDEQLESMIVENVLIKRPYATRVQMLQSLTEQNTTSVDGIDLWELLPIEVRVLF
                     KGDYNVEPVALKKNDILVEILQDENNTPVPFIMEVTKVHGSFHNRYIVGKFYECCLYR
                     GQISPEIQEKINEYVSNFTGEYNS"
     gene            14419..14901
                     /locus_tag="PLU55_00595"
     CDS             14419..14901
                     /locus_tag="PLU55_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86591.1"
                     /translation="MAKLILKKISSIFEEEAPKKKIDAKKTVKVLESSIKSLKTARMS
                     LVGIKSKLKEEDGENPNEAVDALADTIDSIESAISDIIDATSATNPAVQTLIGTVDTL
                     EAEKDNAEIGAMLSGEGDEFTEETEDEEDEEEETADEEDEKKEESIFKRDRKLLFPKK
                     "
     gene            complement(14974..15648)
                     /locus_tag="PLU55_00600"
     CDS             complement(14974..15648)
                     /locus_tag="PLU55_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86592.1"
                     /translation="MITEISNESKRLTAEGITFKQMYQKSYKFYKLKKGDNKDLRMRM
                     DVTRAKITARKKLEYDRSEKKWIQTGRDIKVEFLVSSEPISYKKRDSINVHKFPVTFI
                     IHDIDKGVNSTFKYRSGSLFKPRFPSKGMSSQQRQAIENYNLKKGIDLWFFFHLEWVL
                     RQENVLYGVNYAKYKPKETNPKSYIYFEKHSLFCFKKILFPLLTSRKQLLKRVLNEER
                     ENFIKK"
     gene            15704..16942
                     /locus_tag="PLU55_00605"
     CDS             15704..16942
                     /locus_tag="PLU55_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86593.1"
                     /translation="MKKITKEELIKEFKEIGKDFKNPLEITRKDYRKVAKNYNAYDNF
                     FKTFDAFKKETFSITEEDEKKFELEKKVLILENKNRELEKEKEALLKSSISEDTIIED
                     FEKLRLKEIIPQKINLTTIKPSSNREAVLVISDIHCGEVVKAEEVGGANEYNLEIMKE
                     RLDRVFKYACYYCKQTNVSIMNLIFDGDLLSGSIHDELARSNECSEVEGLFILQDYIS
                     NKLLEIESIFSKINVHFLVGNHSRLTKKPETKQNAILNWEYVLAKQLHILFNTIQKEK
                     NKKIFINYYQSPFTVLNITGHKFLVTHGHLMMGKGSGGFAGIPYYALSASSAKMYGVL
                     KQIGCDESVTFDSIIMGHLHSTGEINLFNGGTLYMNASVIGTSEYGLYNVRAIGKVEQ
                     TLLILEEGKVNTKYLLRGNE"
     gene            16982..>18935
                     /locus_tag="PLU55_00610"
     CDS             16982..>18935
                     /locus_tag="PLU55_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86594.1"
                     /translation="MKEVNNKLPSKILNQLQSWFGIGFKKNSKEPDKQLFPIVKGKTH
                     TEREKSEEFEKIPFDDNVQELFKYFLNNNRVILENYENRTKLFQDMDKMYMNCPIVNK
                     AIELTVDECMQADSNDQIIFIEAKRQLKKDIQEFFDKIELKDLIRPTLIDIIQYGNAG
                     WILGFDDEGISEVIPVDVYDIKDRMEFTPFEVERELKKGQSTFKKYSGYDRVKELIDS
                     ILEKNNNTSYYKKYLFGFEIGDTIIPPWKFLHFRNMTNKSPFKPFGIPQFINSMSPYM
                     QYDASMSLQQTARGLRFPKGVFKINIPSAVDPVTKINKAIEFMNLLTNSGFGTSKKEM
                     QGLGDIIITIQDLYEYTQEESNIDLGKIDDIQMLKDDIILSTFLPRKWLDPNDSGFGD
                     SGVAMIEQFKPFARVVYRFQSIFLQCLTQLVKLHFIYSGKYKPEEIDFTLSMKYPESQ
                     TNSDIISSQNSLIDLANSVIDNIQDKILGGGDLPTDIKRSIYTHFLPYDSNTIEAWID
                     TAIKEKEKAEKEAEEEGDTSFGNIPSESENNDTNNFEKSETDSETSEKLGSDDDVESK
                     LSGFDSVFEKRKGRLKNYYLLEKSLGKENLKEAINNVLLEKKQEELREYNFGNRHYYS
                     SRNRFSDFDPEMLATFDKRKAKRLKEG"
     assembly_gap    18936..19035
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19107..19250
                     /locus_tag="PLU55_00615"
     CDS             19107..19250
                     /locus_tag="PLU55_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86595.1"
                     /translation="MENMVEVIINNVSTAITMQKFTEYKNSPNYQIIMENGKYKILER
                     MNG"
     gene            19265..19639
                     /locus_tag="PLU55_00620"
     CDS             19265..19639
                     /locus_tag="PLU55_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86596.1"
                     /translation="MILQKDDDEFLNKLSAFSRQDKTVVYDVMRSFIFACGLEIRKKI
                     EDDSEGEVKITIPFLCNLFINFEDELTNKGVVSKVVMRAEPQDAIIDEINSIVGEFET
                     ITEKQLLKQMETKYRKILDISE"
     gene            19636..20001
                     /locus_tag="PLU55_00625"
     CDS             19636..20001
                     /locus_tag="PLU55_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86597.1"
                     /translation="MKWFVYNRIPNDFVVFVSSENQEEKQYKKYDDFLMILQENCNQK
                     LLPSLIEFLNCYRTVYVDLVNQEFKVIPLQDLIRNSGNAFETLVKLNTPEEVVDKGKK
                     IVEKSKEFLQNIFEKRRKL"
     gene            20001..20273
                     /locus_tag="PLU55_00630"
     CDS             20001..20273
                     /locus_tag="PLU55_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86598.1"
                     /translation="MAGSLTIDISEEEEKVYEFTGFEVIKYPDNTYWFMNEEEYFEIT
                     NIIFEKKEGYETVVVFSTTDTLSTFNFPVSEKDKEALVSLWERKKD"
     gene            20280..21389
                     /locus_tag="PLU55_00635"
     CDS             20280..21389
                     /locus_tag="PLU55_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HPJ86599.1"
                     /translation="MEKLNASEIVFCLDRLNIKREPSPNHKNWLGILCPNHTDTNFGS
                     CSINLSNGVISCFACGYKKHITKFISDKFQISYKEAVEYVKGIKTDLKNNPDLKIEVE
                     VEKPKELKTIKLESLLLQEFNPEDYIYTKSRGFTKEFCKEFEIKHCLSKVYKDYFITP
                     IKSKKLNVNTFEARKLRQKEHLAEYYNSGYSNELELRFDNYIDRSRIRLKKNKLLKNG
                     VPFYDEDIYYQLRTKVLYPINSNVCDTLFNYDNLEKNKILFLVEGIGSISKIWSNVSK
                     NVTCFFGVKLSFKQLNLLKEFDTIYHIPDFDEAGYNSVVLLNSELTKNNYNVIDTRVE
                     DTSEYYVSEIKNRKNIIKPSEYLLKYFQNFNTNRL"
     gene            21386..22618
                     /locus_tag="PLU55_00640"
     CDS             21386..22618
                     /locus_tag="PLU55_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86600.1"
                     /translation="MSFLYEIYAKTTPQAIVLGANTENRQVKKLLLKDSIFNFYPYAE
                     LELFDTNSEILNNIFFVEGMPFDIKFGTPEIKGDKNQIISGGYLENKFVWSENVINNG
                     KISNGIIGDNNIVLISSLFMNDRPETRAYNSNISDTVETLLNTQLSALKNDYKRNITP
                     TTGNDSKIFYQAGRKTSDFIKYLAERSYTTTNTKNSNFTSFFNLHNEFYFQSFFDLFN
                     QTPIMEITTEEGETASIDSKYIREVRLHNVGLPISFKNYNQNFHKLRSDGIFEESDKT
                     IKDLSFAINQGDCKIILQDQVNTTTNIKNNGVYENVKDIEFFNSKEQQNLFNTNLFYQ
                     IKVTMRFDRSFVSGKVLKINNKSDNTLPVSFLSGNWLIVESSHLYILDGSCYSTLSLV
                     KPNVKITDKYFNFKNILV"
     gene            22773..23186
                     /gene="tnpA"
                     /locus_tag="PLU55_00645"
     CDS             22773..23186
                     /gene="tnpA"
                     /locus_tag="PLU55_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HPJ86601.1"
                     /translation="MYRLDKKSNCVFSLCYHLIIVVKYRKKVFENIMLVSDVKTIVQE
                     ISESMQVEVIEQECGIDHIHILFRAKPTLDMTKYINLLKGNSSRNIRKKYKSFLKDKL
                     WGDHFWSPSYFLATTGNVTIDILKEYVENQRKEQE"
     gene            23186..24325
                     /locus_tag="PLU55_00650"
     CDS             23186..24325
                     /locus_tag="PLU55_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003353982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPJ86602.1"
                     /translation="MKQNKAYKFRLLPNKTQSNLIDRTIGCARFIYNQMLSDKIKHYK
                     DTKLSLKNTPAQYKNEFEFLKEVDSLALANAQMNLETAYTNFFRKVKQGKKDLGFPKF
                     KSKKKCNWSYTTNNQKESIRIENNKIKLPKIGFVKLIQHRGIANDCIIKSVTVSKTKT
                     NKYYISILVEYETQIINKSIEKVIGLDFSMKELYVDSNGYSSNYPRFFRLAQEKLAKE
                     QRIFSHRKVGSKRYEKQKLKVAKIHERITNQRKDFLHKESHKLANTYDMVCVEDLNMR
                     AMSQCLNFGKSVADNGWGMFRTFLKYKLENQGKQLFVVDKWFPSSKMCNVCGVINKQL
                     TLADREWTCDCGTFHNRDHNSAINLRNVGIKTTGTVGLAQECLCH"
     gene            complement(24437..>24754)
                     /locus_tag="PLU55_00655"
     CDS             complement(24437..>24754)
                     /locus_tag="PLU55_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86603.1"
                     /translation="STAETLTNKTFLGYPVYRKVVNFGALPNATTKYVAHGIQTPYTV
                     ISLTGIATNANDYLTLPHVYFGDNTYAVLVQINRNTNQVEVGTAVDRSAYNAKIIIEY
                     IKN"
BASE COUNT         9168 a         3256 c         3429 g         8801 t
ORIGIN      
        1 tttcttcaag attatatttt tcccaccatg ttctaaagtc ttccatatag tcaaagccta
       61 aaagttcttt tgccaaacct acagaaatta gaggttcttt cccttccatt gcaaggtaca
      121 ccaaaaattc aagattttcc ctatcagtca ttgttttttc caattagtaa tttgttatat
      181 agtttaatac agtcatctct caataaaggg gaattgaaat taattacaaa tctcccccat
      241 gtataaacaa attctgaaac tttaatcaaa gatttttcct tcaatacatc ataattaatt
      301 tcaaagaaat taagcattgt ctttccggca taatcaattt caatttgttt ttctgtaata
      361 tcatgtaatt ttacagaatc attttttata tattcatatc tttctttttc tgaaagagtt
      421 cttagtttct ccataaattt acttacagga acggcatttg ttgtacatat agatgttctt
      481 ttttctaaat taccattata gttaagaaga aaacaatcat ctttaagata tattttttct
      541 actacaccat aatgtcttac ataagatttt tttcttagca aaagaacatc gttaagattc
      601 attccttctt ttatttttgt tttcatttct tcttcagaaa gttcaacttc taattcttcc
      661 atataatcaa aggaaggata gaatgtttta agccattttt caacttctgt tatataatca
      721 taaaatgata tatcattact tataggataa tattttttat ttgtacctat ggaaaaacca
      781 atagacattt cggtattatc taaaattgta ataacatgaa taggtgtgtt tttaagaaag
      841 gaagctatgc gataacaaag tccttcaaga tagtataagt cattgtattt agactttgat
      901 ataaattctt tatcagtcaa ccaaaaattc aaatttcttt cactcataaa ccgcatagcc
      961 cccttgctct atatgttaat cacgtctaaa gatattgtgt atatatagtt tgttagtatc
     1021 atcaccaata aagttgatta ctttgttttc caaactacac atcatcttca aacttttact
     1081 tgtaatacaa ttaataattt taagtaccca ttctgtagaa agaataaaag aaaattcttc
     1141 aagttcagta ctataagaaa catttaattt ttctactgca tactttccat ctttagactc
     1201 aataaccaat tcattttctt tacaagtgaa aaacaaacga gtgtctatat tgtctttaga
     1261 tataatttta attcgattaa gaatattttt aaattctgcc ttatctattt caatagaaaa
     1321 ttcattgttg tatcttgctt ttttatctgg ttcaagaaga tttggtagtc taatattttt
     1381 tgattttttc aaaacaaaaa cagtattatt tactttaaaa gaataaaaat cttcaaattc
     1441 tttaatacct attgattcaa ttttcaatga agatagaaaa gtaaatacat cataagccac
     1501 actaaaacct tctggtataa cattttcaga atttctatta aatattgcag tagctttttt
     1561 ctgattgctt gcaatataat gacctgaata atgaatcacc ccattgagat tatcttccac
     1621 tgcacctaca aaatttccaa gttcaggaaa cattgagata ttatcaagtg tgaaagctac
     1681 ctcttcattt tcaatctttt ctctataatg attaaattct gacggaatta catcaaacaa
     1741 tggaaattca tatttgcttt ttccaaattt aattgtattg tctgccaatt caatttctac
     1801 atcatcttta attccactta gaattgaagt aaattcatta accggataaa cggaaataaa
     1861 atctttttca atatcctttg aaatcaatgt gtatagaatg cttgtgcctg tatcgtttat
     1921 aacaaacact tcttttgttt cagcttttcc tataaactga attttgtcat tcattaaatt
     1981 tttattgtta gcaaatttac tataccaatc aataaaattt aattctgatt ttaatacctt
     2041 catttatttt tctccttttt ttaattctct acttgtccat ctacaatatt gtagtttgta
     2101 gtttctacta aattattaag atattctttt gtagcattac aatgacttat aatccatatt
     2161 tgattaaatc ctttttcatt tagtatagtt tgaaataatt taacactatt ttcatccgaa
     2221 gcattattat ctacttcatc taacacaagt attccaagat tttgaagtga acataaagct
     2281 aatctatttg ctaccgctaa aactgctttt tcctgtccac ttgccattac tacattatta
     2341 agtatatcac ttccaacttc cttataaaag aaatctatac cggatttatc ctgttcaaaa
     2401 gtaatttcat attttccata acctttctgg aaaatactat tcattcgatt tttaatatgt
     2461 tcagcacctt tatttattct ataagctgaa aaatctttgt aggcatctct tacttcttcc
     2521 ataagattta tttcttttct tttactttca agctctcgtt ttttaacaga aacaatcctt
     2581 tcattcttag acttcttttt taaaacttca tcattaaaca atttaattct ttctaattct
     2641 tttacctgat tatcatataa aagaatttta ttctttaaat ctgtatattt tttttctttt
     2701 gaaaaagctt ctctttccaa aagattattt attctttctt gtagtacttc gatttgttta
     2761 tctctttcaa ttgttaaaag atgttcaata gattctattt cttctttttt taatttaatt
     2821 atagattctg tatcagaata atcttcttct acataattgt ctatgacttc ttgatacttt
     2881 ttaatacttt cctttttaag agaaatttta atatccgcat ctaaaatttg tttctctatt
     2941 tgagaattaa actttaaagt ttctttatac tcctttatct cttcagaaag ctgtttataa
     3001 tttgtatcaa gttctactaa ttcagtattt tttgattcca aatctttttc atatttagaa
     3061 acatccacac cttcaagttt ttcacaatca aaagtataag gacattttct atctttaaga
     3121 gattttattt ccttctcaat agattttata ttctgttcta cttcaatttt cttagtgtat
     3181 atttgttgta catactcttc agattgagaa gggtttatta cttctttaat tttttttgct
     3241 atttcttcta cactattgtc tatgtcttta atttcatctt gtattgtctt acttgaaatt
     3301 actgctgaat caaattcatt tttcttgcgc ttaatttctt taagtttatt ttcaagattt
     3361 attatttctg ttttaatgtt agaaatactt tcattctttt tcttttcaaa atttaattta
     3421 atttctgaaa tattttcttt tatttctgaa atttctttat ccaattcctt ttgtttttta
     3481 ttatattctt ctttttcttt ctctataact tcaaattctt ttttaacttc atcaatatcc
     3541 ggtagagcaa tttcatccat taaagaaaac tctgcatttt caagagaaat caattctgct
     3601 tcaagtactt taacgttttc tctaattttt cttacttctt cttttacatt ttcagaagat
     3661 tcattgattg aatcaatttt taatatccga gaaagagtat taagtctttc tgtttttttc
     3721 tgaaataata actgtgctgt tttcccttgt tcacttatag cagaaaactt tgttaattca
     3781 aaatctataa tatctctcaa tttagaggta gcctccgaat taaaatattc ttcatcatta
     3841 aatgttaatc tttttttagc acccttactt ccttctattt caagagtata ttcaattcct
     3901 ttatgttcaa aaacacaaag tatttcaaat ttttcagtac cccatcttac aaaagatgat
     3961 attttgtcat caagttcatc tgttaaaata tatcttatag catcaagaat agatgattta
     4021 ccattaccat tttctcctgt tataagattt accttactat tattatcaaa tagaatatgt
     4081 ttaattgctt taaagttttt aatttcaagt atgtttatct tcaccttttt tctccttgaa
     4141 tagtgttgaa caccaaataa catttctatt atttggtgtc aaaccatttt ttaattttct
     4201 tctactgttt ctgcttcaat aacttcttca gttttaactt ctacaggtgt tgatatttct
     4261 tcaacgccaa ttattccttc ttcttttttt gaacggcgtt ttgtagtttt agtatctgta
     4321 gaaatagctt ctttaatttt agcgttaaat gaattttctt taggttttgt tattttcaat
     4381 acagaatcat aaacagcatt tgtaatttct tcaagccatt taggattatc tattacagtt
     4441 tttaaagcat tatcttttcc ttgtcccaat cgttcatccc cataagaaaa ccatgaccct
     4501 gcttttttaa caatagcatt atctatagca agtgtaaaaa tttcctcatt cttatcaata
     4561 ccatattttc cattcttacc aagataaatg tttgtttcta cctttctaaa aggaacacca
     4621 catttatttt ttactacttt aatacgtgta agaaatccat ctacatcttc tttatcacca
     4681 attacttccg aacgatttac ttcaagttta actgacgcat aaaatttaat agcttcccca
     4741 cctggttctg taataggatt accatacccc ccaattttca ttctctgttg attaattaaa
     4801 acaagtgttg ttttattttt aacaagaatt gaatttattt tatttagtga ctgactcata
     4861 agccttgcta attgtcctat atgattttgg tcatattctc cttcaagtac tgctttaggt
     4921 gtgagagcgg ctactgaatc aacaataatt aatttaatat cccctgactt tgccatcata
     4981 tcagcaagag caaaagcttc atcaccacta ttaggctggc tgattaaaag tttttcatta
     5041 tcaaccccca ttttttcact ccatattggg tcataactat tctctgcatc aatataacca
     5101 caattatatc ctgcttcttg tgcttcccta accatatgca aacacagcaa acttttaccc
     5161 cctgaaggca ttccagtaat accaataatt ttaccttcag gaataccacc acaacctaag
     5221 gcataatcaa gtaaaattga gcctgtacta caaataattg gtgatacagt acaattactt
     5281 ccttgaatta aagaaccttc accaaatttt ttacttgctt cattcattaa cccttttaaa
     5341 tctcccattt ataaaatctc cttatttgta tttgcatata tctttcatat aattcattat
     5401 atttctgttc acctataatt cttttccaat aattttgtga ttgttcataa ctcataggtt
     5461 tttgtgttat agggtcaatc aaatggtcaa ttgcagaatg taccccccga taaagagtat
     5521 aaacattatc agtatcataa atcatttcag ggcattttga ccttgccaaa acatgagcac
     5581 catctcttac agctaattta ggattttcta aaatcatttt atctattact tctctttcct
     5641 ctttagtagc aattgcataa aaacggcatt catttttatc acgaattaaa atctctcttt
     5701 tcaattcttc ccacttaggg gtcacttctt tttgttttct tttttctaca tctttttctg
     5761 attttaaaat gtatttttta taacaatctt cttgtttgcc ttttgtaata caccttttac
     5821 tttttattgt ttgtcttaaa caaaaacccg attcacactt ttttataaaa tcttcaaatt
     5881 caatcattat accacctaaa atagtaaagg gggtatttaa cccccttgtt aaaattatct
     5941 gataccacat accggacaag taaaaatact ttctggaaaa atagtatcta tttcagaatt
     6001 tggaaattta catttttcat ccttgcatcc aagaagttcc ccctctttat tattaaaaag
     6061 aggaatacca tctttaattt ctgataaagc atttaaaaga gaagcttttt cttcatccga
     6121 aagtttatca taagaaggaa agtatttcac ataagaaatt tcttcttttg tttcttcttt
     6181 cttaggttct tcaactcttc ttcttttagg ttcttcttgt tttacttcag gttttggttc
     6241 ttctttaaca ggtgcttcca tttcttttac atcatcagaa acttcttccc cttctacctg
     6301 ttccttttct gcctgtgctt ctttttttgc atttctttca tcttcttcaa ttttagcaag
     6361 tgcttcaagt tcaaccataa aatttgaacc tgtttgagca tctacttttt ttattagcca
     6421 tgaaagccac tttttaattt ttgttgctga agaagcataa ggaaaatatt tatcaaagtc
     6481 ataaagttca taagattttt cttcatctgt caaatcagaa tccaaaccaa tgtctttact
     6541 tttctgagta ataaatttag ggcaagcatc tgtaatatcc gcaactgtat aatctttttc
     6601 tacttttttt acaattgtat caacatcaaa atttcctgtt ccttttgcat gatattctac
     6661 aattttgtca taagtagttt tagggatacc tgtatccgta tagaatattg taatatcctg
     6721 atttgtttta ctgtctttaa ttgaaaaacc ttcatgtttt gaagtaagaa tttttgtatg
     6781 cttattatcc ttacaccacg aatcatgtct atcaataaca tttattacta cacgttttga
     6841 gggataaaaa ttcttaggaa atttttcacc aatttttgca ttacctactt tatctacaat
     6901 tattttataa acttcggtat ctttataata atgaatatac tctccgtttt taccagttag
     6961 ctcatttact tttccttcag gatatttttc ccatcgacct tctttaacct tattaattac
     7021 tttggtgaga ataaaatctt tatctaaaac ataatcacct tttgtgtcat cataatgata
     7081 aggaaaatta aatttgacat accccttttt atcttccttt acaatttctg actgcataat
     7141 cattttaatg tcagtaggtt ttcggttagt agggtcattg taaggaaaag gctttccaac
     7201 aattctaaaa actgtttcct cttttgtaat gggacagtaa gagagttcat caaatccctg
     7261 aaaatttcct gaattttctt ttgctctttt tttctgttct tcctgctttt ttcgctgttc
     7321 aagctgttct tgtaggatac tcataaatgt atctcctcca gtttaaatta aaatttttag
     7381 tgcattttta gtacactatg ctaaggtggg tggggtcgaa ccaccgatga tagtaccaaa
     7441 aactattgcc ttaccacttg gcgacacctt aataattttg tactttttag tacaattaac
     7501 aaaacattga aaaaattgct tgaaaaaaat caagtacttt ttcatttttc ttcttaattt
     7561 tcagaaattt tgaggtattt ttcaatgtgt actaatgaaa agacataaaa aagtgaaaaa
     7621 aatttggatt tttgaaaaaa aatttaaaaa aatgaggata ttttgaaata tttaactttt
     7681 tggtgatact aatataaaag atagatttta gaactacaaa agaccaacac ccttttttgt
     7741 agttttaaaa ttttaaaaac gaataagcct tcaaaaacat agtgggtgta caagattaaa
     7801 agaaatttta attttggact atgtttttgg aggttttttt gtgttcattt ttaaattgag
     7861 gtgatgacaa acgagttacc aaacgaaata ttatgaaagt aaatgtccta tttatgatag
     7921 agaacatact gaggagtttt ctaagttcat aaaagacctt aataatgatt tgaaatcctt
     7981 gcctgtttca aaacatcata actatgtcaa aaacaaatat cctgaaataa ttaaattctt
     8041 ggaaagtctt ggttataatt taaaaaataa ttttattttt caaaaaacaa aaaaagtaag
     8101 aaatcatgta aacacttcat ggacagaaaa taaaacaaca ccttttgaca ataatttaat
     8161 tgtattggaa aatgatttat ataatggaaa aacaaaagaa tattataaaa aaaatattta
     8221 tgtaagtaaa atttctttaa taatcaatca tatcttttca gatatacttt caactgatta
     8281 tcattactcg gtaattgata atattcttga atcagaatta gccggaaaaa atataaataa
     8341 aataaataaa aatgtagggt gttatttcaa agagaaatca aatgttcttg ttatagacat
     8401 tgacaaccat gataataaaa acactttact tatgcttgaa acttatcaat ctacattaga
     8461 tttttttgga aaagataata taatctttca ggaaaattcc tattcttatg gctatcattt
     8521 atttattaaa cttaataccg ccttaactat atctgaaaag aaaaaattaa ttaaagatta
     8581 taaaaaatca cttcataaat attctaaatt tgtagaatca cctttaaaaa tgagacttcc
     8641 tataagttat gactatactg ttcttgataa taaatacagt acgagtgcgg actataataa
     8701 taattctcaa aattgtattc aagaaacaat tcttgcttat gatacttcaa aaggttttga
     8761 gtgtgaaagt tattctgtaa atacaacaaa agaaaaagct ataaatgaaa tctattgtag
     8821 agaaactata aacaattcac tttctaatat gagctatgaa caatttcttc aagatgaaaa
     8881 atttgattta tattcgggtg aaagattttg taaaatgcct ttttttgttt cttttgcaaa
     8941 atcacataat ttatctgagg aagaatgttt tgatcttgta attctcaaag gaaaaggttc
     9001 taaagatgtt ttatccaatc ataagaccat aaaatcggaa ataaaatctc tatataaaaa
     9061 ttataattct caatcaatca aatatgatgt taacaaagta caagaatact tttccaatat
     9121 agataaaatt cctttagcag ttgtaaataa agtaactcac aaaaaagtgc ttattcaaat
     9181 acttgttaat actaagcaaa aagtatctaa tcacaatatg gaaatattaa aaatacttct
     9241 tctggaaatt ttaggtaaat tcttctataa tttgcacaat cgaaaagcaa tcaaaagtaa
     9301 taattacacc gataaatttc tcataggtga acagtattca agaaacatgt gcaatcttct
     9361 caaagaaaaa tatgaaatat taaaaaatgt aaatgtttac aaacgaattt cagatattct
     9421 tctatactct tgcttattct cacaacaatt ctctaataaa aaaggatatt attgtgatta
     9481 tgaaaataca ggtaaatctt tctgtagaca attttatctt aaagctaata aagataaaga
     9541 aattaaattt gatatagata gattaatact attcattaat gaatccattg attatctctt
     9601 taataaaata aaaacctttt caattgtaaa ctggaaagaa tgtatcttta actctataaa
     9661 ggaaattaac tctattgttc acattttttc tgagggaagt actgttaatc acaatataat
     9721 gaatgatttt cagtttcaaa agatgaaaac atgaaagaaa ttagaaagag tactaaaaat
     9781 agtttaaaaa taatgaaatc tttaaaaaag gagagataaa aacatgggta aagctaagaa
     9841 agaaaaagca aaagcaaatt ttgaaagaaa gagtaaaaga gatagtggta aaactataaa
     9901 aactcttggg gattattatt ggagtgtagc aaaagaagaa cttgcaaaag aagtacctta
     9961 tatgatgttt gaaaactaca tgaagaaagg atttatagga aattatattc ttaattttgc
    10021 aaaaaagaaa tttaatctta atacagaaaa agagtggatg agtattaaga caataagttc
    10081 ggtacttttt ctccatgcac ttgaatttga tgttgaaaaa gacatgttta ctttcaatgc
    10141 agaatcggaa tatcaaggat ttatagatta tctggaaagt acaattgtag tcataaatga
    10201 acatgctgaa aaagcaaaag aaatgtcaaa agaggatgaa gaaaaactta gtgactttgt
    10261 taaatttcat actcgttctg atttgatata tgataaagca gtattttctt attttcttga
    10321 gaattttaaa aacaatacta ttaagaaaga gtttattaaa gaagaagtaa aggaggataa
    10381 agaataatgg gaagagaagt agcaaaggaa atatttcaca gatatagatt aaccacggat
    10441 tataaagaaa attttatatg tttgaataga gaaaatctat caaaagattt tattgatttt
    10501 cataatgaaa gaaaagattt tgaaaattac attagaacaa agaaaaatcc tgaaggtatt
    10561 ttggattatg taaaacttga tagaatttca aataataaag aatatgacag tttctattat
    10621 tatgtaaaag aacctgaaaa atattatagt tatcaggaac tttgtaatat gaatagaaaa
    10681 gaattggaaa caatatcata tatttatgga ttgccaacta ttatggttgt taattctata
    10741 atgattaaaa acatcatgaa agaacaggaa gaatggtttt cagatgaagc tactaaactg
    10801 agagaagaat ctaaaaagga ttccaatagt tttgaggatg cttttaagga tattgtaaag
    10861 caggcagaaa gtcaggctta attttaatac taataatagg agaattaaaa tggttattga
    10921 aggtacatta aaaattaaat ttacagtaaa agatggtgct ttggctctat ccgatctttc
    10981 agaaatgaat aaacgtctcg aaggtatcat agaacagtct gcttctgaaa tagaagaagt
    11041 ttgtgattat cttggctgta aaattatatc aaatgaaaga ccaattacaa ttgatgaaaa
    11101 actttgtatt gtctaggggt acaaaaaatg aagtataaac ttctttcggc taaaagtggt
    11161 aaatcagaac aggaaataaa agaagtacat aaacaagcaa tccaagaagc aaaaattctt
    11221 ggattgaagg ataatcttct tgaagaattt tgtttaactt atattgaaga agagtttgat
    11281 ttagataata ttaaaaaagt aacaactaat ttaaacagag cctttgaaaa aagtaatcat
    11341 gtttgcttta aagactttat ggatgatttg tttgttgaag aaggggaagc ttcttcagct
    11401 acaatgtcta ctgattttcc tgtagaagat agaccacagg gtttcaaaaa gaaaaaggat
    11461 gaagaagaaa aagtaaatgt ctaagaattt taacactaat actgataatc atgatgaatg
    11521 gttaacacca aaatatataa cagacttttt tgaatttgat ttagaccctt gctctccgat
    11581 tcaaagaccg tgggacacag ctaaaaatca ttataataaa atagatgatg gatttagtaa
    11641 agaatgggaa ggttttgttt tctgcaatcc tccatacggc agagaaacat ataggtggtt
    11701 agacaaatgt gctgaacatg gtaatgccat aggtctaact tttgcaagaa ccgagacaat
    11761 tggatttcac aatactgtgt gggataaagc tgatgctctt tttttcttca aaggaagatt
    11821 agcttttcac tatgttgatg gacggaaagg aagttcagca aatgcccccc tcagtattaa
    11881 tagcttatgg tgaaaatgct ttaaataaat taaaaaaatt tagtaatttg tattctggtg
    11941 cttttattaa attagttaaa aattagagga ttaataatta tggcaagtaa acaaaaaaga
    12001 tattatgatg aagttaccat tggtgaatat ataccttatg tttatcttgc tatagtagaa
    12061 agactacaaa tttatttttg tggtcttttc tatcctgatg aagattttga agaatctaaa
    12121 aaaagattta tacttgctga ttttgttggt ggtggaaatg aaactgctat aagaaattca
    12181 attgaagctt ataaaacttc acaaggtaaa tttccattta ctgcatataa tatagatgat
    12241 gatgaacctg tagaatataa atcactaaca catttaacag gtcaatttta tgataaagaa
    12301 cttgattgct atatagcctc agtaccggct tatttgaata taagtatgat gaccgtttat
    12361 actactgagt atgactattt tattggaaca tccaaattaa tgtatgattt agcggcatta
    12421 acaagacttg aagttcctat tgtagtaaat aataaagaaa cattttttga aattgacata
    12481 gatatggatg ttacaaaagg tgatttagcg tttgcatatc aggaacaaaa agataaaggt
    12541 tcacttttta atatttccca taattgtaaa attaaatttc cttatttgat actttcccag
    12601 agaggatttg taagtgatga tggaaaacaa aaacctgtac ctgtttatcc tgttgataat
    12661 atgattttgt ctttgaaaaa tatgaaagat aaatctgtta tagatgtttt aaatgtaaat
    12721 gttattccag aagtattaag ttcaacacca agcaataatg ctacaaatgt aaatagaact
    12781 tctgcaatag aaataaattt taatacttca atgaatgaag caagtgttat taattcaatg
    12841 gatattgtac cagaaatatg gggaacaaaa gaatggaatg atagtggtac tactcttgct
    12901 attacacctt atgacagtat gaatgctaat acagaatata atatatttat ttcaaaacaa
    12961 gcacaaaatt ttttgacaca tcctttaaaa tcagattttg aattaaaatt ttccacaggt
    13021 tcttgatatg tataaagatg tttttacaga gaaaacaagg attaatccga aagcaataag
    13081 tttaaaattg caaggttaca cttgctcaca tcctgtatta actgaagctg ttataagacc
    13141 taacaattat gcaaaaatgc taactaacta ttttgattat gtttgtaaaa gaaataaaaa
    13201 taaattagag aaaggtttac ctttttttct ttttgaatta aacaaaagat ttagagaata
    13261 taatgttatt tttgaaattg aaaatatagg aagtccttat aatcaaagag gaatacatgt
    13321 aatgagtact tccacttcta ctggaaaaac aattgtaaaa tgtaattata aacttttaga
    13381 atctttagaa aataaagaaa gttataatag attgttacat gatttttttg tacttatagg
    13441 gcatgagcta atacataggg gtcaatttta ttatagaaaa tataaagaat tactaagtta
    13501 tgcttattat aacgaaacaa attattttga agataaagac gaaattatgg cttatgcttg
    13561 gtgtatagtg gaagaattaa ggtttggtgg atataataat gctgatatat taaaaatggt
    13621 ttctgaatct aaaaagttca cagtaataaa aaagtatagc gacgttttta agaacattga
    13681 ggaacagcta tatcacaaaa ataaaaaatt atttaattta tttttgaaat atctttatga
    13741 gtatattaaa gggaatcact ttaaaaatca aataataggt ttaaaataac atgcaatatt
    13801 cagtagttga caaattatct caaaaaatat caccggtaat tcgtaaatta agtgacatta
    13861 atatcaaaag acttttagga acagaaactc aattacttag aatagtaaca tcagagccaa
    13921 atgttatggg tgaatatgat gaacaattag aatcaatgat agtagaaaat gtattaatta
    13981 aacgccccta tgctacaaga gttcaaatgc ttcaatctct tactgaacag aatacaacaa
    14041 gtgttgatgg tatagattta tgggaattat tacctattga ggttcgagta ctttttaaag
    14101 gtgactataa tgtagaacct gttgccttaa agaaaaatga tatacttgtt gaaatactac
    14161 aggatgaaaa caatacacct gttcctttta ttatggaagt tacaaaagtt catggttctt
    14221 tccataatag atatattgtt ggtaaatttt atgaatgttg tttgtataga ggtcaaattt
    14281 ctcctgaaat acaggaaaaa ataaatgaat atgtttcaaa tttcacaggg gaatataatt
    14341 cttaacaatt tattgaaaaa atacatacta ataaaatgta taaaattttg taatatttaa
    14401 aatgaggtaa ataataaaat ggcaaaacta attctcaaaa aaatttcttc tatatttgaa
    14461 gaagaagcac caaagaaaaa aatagatgct aagaaaacag taaaggttct tgaatcttct
    14521 attaagagtc ttaaaactgc aagaatgtcc cttgtaggta ttaaatcaaa acttaaagaa
    14581 gaagatggtg aaaaccctaa tgaagcagta gatgctcttg cagatactat tgattcaatc
    14641 gaaagtgcaa taagtgatat tattgatgct acttcagcga ctaatcctgc tgttcagact
    14701 cttataggaa ctgttgatac acttgaagcc gaaaaagata atgctgaaat tggtgcaatg
    14761 ctttcaggtg aaggtgatga gttcacagaa gaaacagagg atgaagaaga cgaggaagaa
    14821 gaaactgctg atgaagaaga tgaaaagaaa gaagaatcta tttttaaaag agacagaaaa
    14881 ttattgtttc caaaaaaata ataaatagta gtctatgtac catgagtaca aaagatagtg
    14941 aagccgatgt tcttttgaat gtcggcttct tttttatttc tttataaaat tttctctttc
    15001 ttcatttaac accctcttta ataattgttt tcttgatgtc aataaaggaa ataatatttt
    15061 tttaaaacaa aacaaactgt gcttttcaaa ataaatataa cttttaggat ttgtttcttt
    15121 aggtttgtat ttagcataat tcacaccata taaaacattt tcctgtctta atacccattc
    15181 aaggtgaaaa aagaaccaaa ggtctatacc ttttttcaaa ttataatttt ctattgcttg
    15241 cctttgttgt gatgacattc cttttgacgg aaatcttggt ttaaataaag agcctgaacg
    15301 atatttaaat gtactgttaa cccctttatc aatatcatga ataataaaag ttactggaaa
    15361 tttgtgaaca ttgatactgt ctcttttttt ataagatatt ggttcggatg aaacaagaaa
    15421 ttctacttta atgtctcttc ctgtttgaat ccattttttt tcacttctat catactctaa
    15481 tttttttctt gctgtaattt ttgctcttgt aacgtccatt ctcattctaa ggtctttatt
    15541 gtcacctttt ttaagtttat agaacttata agatttttga tacatttgtt taaatgtaat
    15601 cccttcagcg gttagtcttt ttgactcatt cgatatttct gtaatcatgg tttattagta
    15661 gggatactaa taatttatat tttattttgg agttaaatta ttcatgaaaa aaataacaaa
    15721 agaagaattg ataaaagaat ttaaggaaat aggtaaagat tttaaaaatc ctttggaaat
    15781 aacaagaaaa gattatcgaa aagtagcaaa aaactataat gcttatgata acttttttaa
    15841 aacatttgac gcttttaaaa aagaaacttt ttctataact gaagaagatg aaaagaaatt
    15901 tgaattagaa aaaaaagtat taattcttga aaataagaac agagaacttg aaaaagaaaa
    15961 agaagcactt cttaaaagta gtatttctga agatacaatt atagaagatt ttgaaaaact
    16021 aagacttaaa gaaattattc cacagaaaat aaatttaaca actataaaac cttcttcaaa
    16081 tagagaggct gtattagtaa tttcggatat acattgtggg gaagtagtta aggctgaaga
    16141 agttggtgga gctaatgagt ataatcttga aataatgaag gaacgtcttg acagagtttt
    16201 taaatatgct tgttattatt gtaaacaaac aaatgtttca ataatgaatc taatttttga
    16261 tggtgattta ctttcaggta gcatacatga tgaacttgca cggtctaatg aatgttctga
    16321 ggtagaaggg ctttttattc ttcaagatta tatttccaat aaacttttgg aaattgaaag
    16381 tattttctca aaaataaatg ttcattttct tgttggtaat cattcaaggc ttacaaaaaa
    16441 gcctgaaaca aaacagaatg ctatactgaa ttgggagtat gttcttgcaa aacagcttca
    16501 tattcttttt aatacgattc agaaagaaaa aaataaaaaa atatttataa attattatca
    16561 atctcctttt actgttctta atattacagg gcataaattt cttgttacac atgggcattt
    16621 aatgatgggt aaaggaagtg gtggttttgc cggaatacct tattatgctc tttcagcttc
    16681 ttcagcaaaa atgtatggtg ttttaaaaca gataggttgt gatgaatcgg taacttttga
    16741 ttcaattatt atggggcact tacactctac aggtgaaatt aatcttttta atggtggtac
    16801 actttatatg aatgcttctg tcataggaac atctgaatat ggattatata atgttagagc
    16861 aataggaaaa gtagaacaaa ctcttttaat tttggaagaa ggaaaagtaa atacaaaata
    16921 tcttttgagg gggaatgagt gatttagtac tttttaagta ctaataacag aaagaggaaa
    16981 tatgaaagaa gttaacaata aattaccaag taaaatattg aatcagttac aatcttggtt
    17041 tggtataggg tttaaaaaaa atagtaaaga accagataaa caattatttc caatagttaa
    17101 aggcaaaaca catactgaaa gagaaaaatc agaggaattt gaaaaaatac cttttgatga
    17161 taatgttcaa gaactgttta aatacttttt aaataataat agagttattc ttgaaaatta
    17221 tgaaaacaga acaaaacttt ttcaggatat ggacaaaatg tatatgaatt gtcctattgt
    17281 taataaagca atagaattga cagttgatga atgtatgcaa gctgattcta atgaccaaat
    17341 aatctttatt gaagcaaaaa gacaacttaa aaaagatata caagaatttt ttgataaaat
    17401 agaattaaaa gacttaatcc gtccaacact tattgatata attcaatatg gtaatgcagg
    17461 atggatttta ggatttgatg atgaaggaat tagtgaagta attcctgttg atgtttatga
    17521 tattaaagac agaatggaat ttaccccttt tgaagtagaa agagaattaa aaaaaggaca
    17581 atcaacattt aaaaaatatt cggggtatga cagagttaaa gaacttatag attcaatact
    17641 tgaaaaaaat aataacacat catattataa aaaatatctt tttggatttg aaattggtga
    17701 tactataatt cctccgtgga agtttctaca ttttagaaat atgacaaata aaagtccttt
    17761 caaacctttt ggtattccac aatttataaa ttctatgtca ccttatatgc aatatgatgc
    17821 ttctatgagt ttgcaacaaa ctgcaagggg tttacgtttt cctaaagggg tatttaaaat
    17881 aaatatacct tcagcagttg accctgtaac caaaataaat aaagctattg aatttatgaa
    17941 tttattgact aattcaggat ttggtacttc taaaaaagaa atgcaaggtc ttggagatat
    18001 aataattaca attcaagatt tgtatgaata tacacaagaa gaaagtaata ttgatttagg
    18061 aaaaatagat gatattcaga tgttgaaaga tgatataatt ctttctactt ttcttccaag
    18121 aaaatggctt gacccaaatg acagtggttt tggtgacagt ggtgttgcta tgatagaaca
    18181 atttaaacca tttgcaaggg ttgtatatcg ttttcagagt atatttttac aatgtttgac
    18241 tcaattagta aaacttcatt ttatttattc cggtaaatac aaaccagaag aaattgattt
    18301 tactttatca atgaaatatc ctgaatctca aactaattca gatattatct catcacaaaa
    18361 tagtttaatt gatttagcaa acagtgtaat tgataatata caggataaaa ttcttggtgg
    18421 tggtgactta cctactgaca ttaagagaag catctacact cattttcttc cttatgattc
    18481 aaacacaatt gaagcttgga ttgataccgc aattaaagaa aaagaaaaag ccgaaaaaga
    18541 agcggaagaa gaaggtgata caagttttgg taatattcct tctgaaagtg aaaacaatga
    18601 tacaaataat tttgaaaaaa gtgaaactga ttctgaaact tcagaaaaac taggtagtga
    18661 tgatgatgtt gaatcaaaac tttcaggatt tgatagtgtc tttgaaaaaa gaaaaggaag
    18721 attaaaaaat tattatcttt tggaaaaatc tttagggaag gaaaatttaa aagaagctat
    18781 aaataatgta cttcttgaaa aaaaacaaga agaattaaga gaatataatt ttggaaatag
    18841 acattattat tcttcaagaa acagattttc agattttgac cctgaaatgt tagctacctt
    18901 tgataaacga aaagcaaaaa gattgaaaga aggaannnnn nnnnnnnnnn nnnnnnnnnn
    18961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19021 nnnnnnnnnn nnnnngaaaa aatgtcttga ctttccctga cttatattaa agtattgttt
    19081 taaatttata tttagaggaa aaaataatgg aaaacatggt agaagttata atcaataatg
    19141 tttcaactgc tataacaatg cagaaattca cagagtacaa aaatagtcca aattatcaga
    19201 ttattatgga gaatggaaaa tataaaattc ttgaaagaat gaatggttaa aaaggagaaa
    19261 atttgtgata ctacaaaaag atgatgatga atttcttaat aagttatctg cattttccag
    19321 acaggataaa actgttgtat atgatgttat gcgcagtttt atttttgctt gtggtctgga
    19381 aataagaaag aaaatagaag atgattcgga aggtgaagtt aaaataacta taccttttct
    19441 atgtaatttg tttattaatt ttgaagatga attaacaaac aaaggtgtag ttagtaaagt
    19501 tgtaatgaga gcagagccac aggatgctat tatagatgaa ataaatagta ttgtaggtga
    19561 atttgaaact ataactgaaa aacaattatt gaaacaaatg gaaacaaaat acagaaagat
    19621 tttggatatt tccgaatgaa atggtttgta tataacagaa tacctaatga ttttgttgta
    19681 tttgtttctt cagaaaatca agaagaaaaa caatataaaa aatatgatga ttttcttatg
    19741 atacttcaag aaaattgcaa tcagaaatta cttccttctt taattgaatt tttaaattgc
    19801 tatagaactg tttatgttga cttagtaaat caagaattta aagttattcc tttacaggat
    19861 ttaattagaa attcaggaaa tgcttttgaa acacttgtaa aattaaatac acctgaagaa
    19921 gttgttgaca agggaaagaa aatagtagaa aagtctaaag agtttttaca aaatattttt
    19981 gaaaaaagga gaaaattgta atggcaggtt ctttaacaat tgacatttcg gaagaagaag
    20041 aaaaagttta tgaatttaca ggttttgaag taataaaata tcctgacaat acttattggt
    20101 ttatgaatga agaggaatat tttgaaataa caaacataat ttttgaaaaa aaagaaggct
    20161 atgaaactgt agtagttttt tcaactactg atactttgtc tacattcaat tttcctgttt
    20221 ctgagaaaga taaagaagca ttagtatcat tgtgggaaag aaaaaaggat tgattcattt
    20281 tggaaaaatt aaatgcttct gaaattgttt tttgcttaga tagacttaat attaaaagag
    20341 aaccctcacc taaccataaa aattggttag gaatattatg tcctaatcat actgatacaa
    20401 attttggttc atgttccatt aatctaagta atggtgtaat ttcttgtttt gcttgcggtt
    20461 ataaaaaaca tataacaaaa tttatttcag ataagtttca aatttcgtat aaagaagctg
    20521 ttgaatatgt taaaggaatt aaaacagatt taaaaaataa tcctgattta aaaatagaag
    20581 ttgaagttga aaaaccaaaa gaattaaaaa ctataaagct tgaaagtctt ttgcttcaag
    20641 aatttaatcc tgaagattat atttatacaa agtcaagagg atttaccaaa gaattttgta
    20701 aagaatttga aataaaacat tgtctttcta aagtgtataa ggattatttt attaccccta
    20761 ttaaaagtaa aaaattaaat gtaaatactt ttgaagcacg aaaattaaga caaaaagaac
    20821 atcttgccga atactataac agtggctact ccaatgaact tgaattaaga tttgataatt
    20881 atattgatag aagcagaata agattgaaaa aaaataaatt attaaaaaat ggtgtacctt
    20941 tctatgatga agatatttat tatcagttaa gaactaaagt tttataccct attaacagta
    21001 atgtgtgtga tactcttttt aattatgata atttagaaaa aaataaaatt ttattccttg
    21061 tggaaggaat aggaagtatt tctaaaatat ggtcaaatgt ttctaaaaat gttacttgtt
    21121 tttttggtgt taaattatct tttaagcaat taaacttatt aaaagaattt gatacaattt
    21181 atcatattcc tgattttgat gaagcaggat ataattcagt agtactttta aattcagaat
    21241 taacaaaaaa caattacaat gttattgata caagagtaga agacacaagt gaatattatg
    21301 tttccgaaat aaaaaacaga aaaaatataa ttaaaccctc agaatatctt ttgaaatact
    21361 ttcagaattt taatactaat agattatgag ttttctttac gaaatatatg caaaaacaac
    21421 accacaagca atcgttcttg gtgctaatac tgaaaatagg caagttaaaa aactcttgtt
    21481 aaaagatagt atttttaatt tctatcctta tgccgaatta gaattatttg atacaaatag
    21541 tgaaatttta aataatattt tctttgttga aggaatgcca tttgatatta aatttggaac
    21601 tcctgaaatt aaaggggata aaaaccaaat aatatccggt ggttacttag aaaataaatt
    21661 tgtttggtca gaaaatgtaa taaataatgg aaaaatttca aatggtatta ttggtgataa
    21721 caatattgtt ttaatttcat cattgtttat gaacgataga ccagaaacaa gagcatataa
    21781 ttctaacata tctgatacag tagaaacttt acttaataca caattaagtg cactcaaaaa
    21841 tgattataaa agaaacataa caccaactac aggaaatgat tctaaaatat tttatcaagc
    21901 aggaaggaaa acttctgatt ttataaaata tcttgcagaa agaagctata ctacaactaa
    21961 tacaaaaaat tctaatttta catctttttt taatttacat aatgaatttt attttcaatc
    22021 tttttttgac ctgtttaatc aaacacctat tatggaaata actacagaag aaggtgaaac
    22081 agcttcaata gatagtaaat atattagaga agtaagatta cataatgtag gtcttccaat
    22141 atcatttaaa aattataatc aaaattttca taaattaaga agtgatggta tatttgagga
    22201 aagtgataaa acaattaaag atttatcttt tgctataaat caaggggatt gtaaaataat
    22261 attacaagac caagttaaca caacaaccaa tataaaaaat aatggtgttt atgaaaatgt
    22321 aaaagatata gaatttttca attccaaaga acaacaaaac ttatttaata caaatttatt
    22381 ttatcaaata aaagtaacaa tgagatttga caggtctttt gtttcaggaa aagttttaaa
    22441 aataaataat aaaagtgata atacacttcc agttagtttt ctttctggta attggttaat
    22501 agtggaaagt tcacatttgt atatattaga tggaagctgt tattccacac ttagtttagt
    22561 taaaccaaat gtgaaaataa cagataaata ttttaatttt aaaaatattt tagtttaacc
    22621 tgaaaaacaa agaaatcccc tacctttagg tgggggatga atttggataa aaattgtaat
    22681 tttttcttga ttttttatac taataagggt atattaaaaa taaggttact aatattttga
    22741 aatgacctat actaatataa taaagaggaa aaatgtatag attagacaaa aaaagtaatt
    22801 gtgtattttc actttgttac catcttatta ttgtagttaa atatagaaaa aaagtatttg
    22861 aaaatataat gcttgtttct gatgttaaaa caatagttca agaaatatct gaatctatgc
    22921 aagtagaagt catagaacag gaatgtggaa tagatcacat acatattcta tttagagcta
    22981 aaccaacact tgatatgact aaatatatta atttattaaa aggaaacagt tcaagaaata
    23041 tccgaaaaaa atataaaagt tttctaaagg ataaattatg gggggatcat ttttggagtc
    23101 cctcatattt tttagcaact acaggaaatg tcactattga tatattgaaa gagtatgttg
    23161 aaaatcaaag aaaagaacag gaataatgaa acagaataag gcatataaat ttagattatt
    23221 accaaataaa acacagtcta atcttattga cagaacaata ggctgtgctc gttttatcta
    23281 taatcaaatg ctttctgata aaattaaaca ctataaagat acaaaacttt ctttgaaaaa
    23341 tactcctgca caatataaaa acgaatttga atttctcaaa gaagttgact cccttgcttt
    23401 agcaaatgct caaatgaatt tagaaaccgc ttatacgaat ttttttcgga aagtaaaaca
    23461 agggaaaaaa gatttaggtt tccctaaatt caaatcaaaa aagaaatgta attggagtta
    23521 tactacaaat aatcaaaaag agtctatacg aatagaaaac aataaaatca aattacctaa
    23581 aataggattt gtaaaattaa tacaacacag aggtattgca aatgattgta taataaaatc
    23641 tgtaacagtt agtaaaacaa aaactaataa atattatatt tcaatacttg ttgaatatga
    23701 aacacaaata attaacaaga gcattgaaaa agtaatagga ttagatttct caatgaaaga
    23761 gttgtatgtt gactcaaatg gttattcatc taattaccca agattcttta ggttagctca
    23821 agaaaaatta gcaaaagaac agagaatttt ttctcataga aaagtaggaa gtaaacgata
    23881 tgagaaacaa aaattaaaag ttgctaaaat acatgagcgc ataactaatc aaagaaaaga
    23941 cttcttgcat aaagaatctc ataaattagc aaatacttac gatatggttt gtgtagaaga
    24001 tttgaacatg cgggcaatga gtcaatgtct aaattttggt aagtcagttg cagataatgg
    24061 ctggggaatg tttagaactt ttctaaaata taaattagaa aatcaaggga aacaattatt
    24121 tgttgttgac aagtggtttc caagttctaa aatgtgtaat gtttgtggag taataaataa
    24181 acaactaact cttgccgata gggaatggac ttgcgattgt ggtacttttc ataatagaga
    24241 tcataattct gcaataaatc tacgaaatgt aggaattaaa accacaggaa ctgtggggtt
    24301 agctcaggaa tgcttatgtc attagacata tcgactgaga agcttctgcc tttaggcaga
    24361 agtgagttca caatacagtt gaaacaatca ttccgccgat agtgcaaatt tgtttgattt
    24421 ttttgtcaac aattatttaa ttctttatat attctattat gatttttgcg ttatacgcag
    24481 agcggtccac ggctgtacca acttccactt gattagtatt tcgattaatt tgcacaagta
    24541 ctgcatatgt gttatcgccg aaatatacat gtggtaaagt caaataatca ttggcattag
    24601 ttgcaatacc tgttaaactt ataactgtat acggcgtttg tataccatga gcgacatact
    24661 tagttgtagc attcggcaac gccccgaaat taacaacttt tcgataaaca ggataaccaa
    24721 ggaaagtctt gtttgtcagc gtctcggctg ttga
//
LOCUS       DAOPCH010000005        23301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_2343_length_23301_cov_5.129528, whole genome shotgun sequence.
ACCESSION   DAOPCH010000005 DAOPCH010000000
VERSION     DAOPCH010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 23301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23301
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2343_length_23301_cov_5.129528"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLU55_00660"
     CDS             complement(<1..376)
                     /locus_tag="PLU55_00660"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HPJ86604.1"
                     /translation="MEENKEIGDLFIPQGEQEKTVVEKDFMDVLKMVAPGTSIRVAMD
                     DLLNAGMGALIVIDNGNISSAYQKGFGIHSKFTPQKLVELAKMDGAIILSKNVKKILY
                     ANSFLSPNSEITTRETGTRHKSA"
     gene            complement(433..960)
                     /locus_tag="PLU55_00665"
     CDS             complement(433..960)
                     /locus_tag="PLU55_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HPJ86605.1"
                     /translation="MKVAIGTENKAKIKAVESIIKEVWKDVEFSYHKTNSLVKDQPLS
                     DEEAIKGAINRAKEAINKGDADYGIGLEGTVNTNNYGMFLHGWVTIVDKYGNIGFGQS
                     ASVHIPKTIEQRIKKGEELGPIIQELMNDEKNSIRHNEGTNGILTGGLYTRVKEFEDA
                     TRCALSRFVAPKWYS"
     gene            complement(1056..2057)
                     /locus_tag="PLU55_00670"
     CDS             complement(1056..2057)
                     /locus_tag="PLU55_00670"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013099824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HPJ86606.1"
                     /translation="MKKSIKSQESDVAKNILKKSDKVEGKIIEGYDFNKGVDYNKIID
                     SFSNTGFQASNLGEAIDIVNEMIKIKSKIYLGYTSNIVSSGLREVIRYLVEHKKVDVC
                     VTSGGGFEEDIIKCLGETVLGDFRLPGKELREKAINRIGNLLVPNNNYIKFEEFSMPI
                     LEEMYQEQKKTGKVYTPYEIIWKFGEKINNKKSICYWAWKNKIPIYCPAILDGSFGDM
                     VYFFKYKHEDFKVDIAEETRQLNESTMGIEKSGVLIVGAGIVKHAILNAHLYRNGADY
                     AVYINNAQEFDGSDAGALPEEAVSWGKLTPECKRVKVYGDATILLPLLVAKTFAKNN"
     gene            2141..2332
                     /locus_tag="PLU55_00675"
     CDS             2141..2332
                     /locus_tag="PLU55_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86607.1"
                     /translation="MNKWLELILGVILLVGVVALVFPGMPMQSWGYAAWTVLKGGLTW
                     IVAITGLVLIILGISEIKG"
     gene            complement(2339..2947)
                     /locus_tag="PLU55_00680"
     CDS             complement(2339..2947)
                     /locus_tag="PLU55_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HPJ86608.1"
                     /translation="MKKRRVNKKIKRNFFSENYKKCWDYLVESRKFIWAIVIIFFSFV
                     AIGFIFQPESISQLIMEMVNELLNKTEDMSYPELTSFIFFNNFKVGFMGVLYGFLFGI
                     PSVLFSLVNGYVVGFVAFFAVSSSGVSSLLSLVPHGIFELPAIFISFGLGFKFGTFWF
                     YKEKMKNFSLFFVESLRVFIFVVVPLLIIAAIIEGTFIHFLG"
     gene            complement(2978..3649)
                     /locus_tag="PLU55_00685"
     CDS             complement(2978..3649)
                     /locus_tag="PLU55_00685"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPJ86609.1"
                     /translation="MTYVYRINPKKKKNFFDSFSLNTLLIIVNVVLFIIFSVLIKLNF
                     LSVDDIAIMPSNILDGKYLWTFLTSMFMHAGFFHLLVNMLSLFFVGSLIEKIIGRRRY
                     FWFYIFSGIFAGAFFILVSFLFKVDLYSYAVGASGALFGLIGLLMLLTPNLPVYIMFI
                     PIPVKMKYAAPGMLILLWVISLAGNVSIGNTAHLGGLIAGLIYGLFLKSKYRNKTAYI
                     RRYFS"
     gene            complement(3667..4431)
                     /locus_tag="PLU55_00690"
     CDS             complement(3667..4431)
                     /locus_tag="PLU55_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86610.1"
                     /translation="MLKNFLNEVISIVVGKPSDSIVRLLEGDKYVNEFIIAKKLDLTI
                     NQTRNILYKISDYGLVSSIRKKDKKKGWYTYFWKIEVLKSLEFLKELTIKNITQLRNQ
                     IKSRETKGFYVCERCNIEFNEENAMLHDFTCNECGEVFTVKDNEKVIREFKKNEDKLV
                     NKLKLIEEEITTEKARLDKIKMKELKKIEAEKLKQREEKRRIQKEAREKLKKESSKKK
                     PSEKKSSKKKSSTPSKPKKNIKKPSKVKDKKKSKKK"
     gene            4557..5288
                     /locus_tag="PLU55_00695"
     CDS             4557..5288
                     /locus_tag="PLU55_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86611.1"
                     /translation="MGLFGKKDKKEEIKRPVQSVPELPELPKLPEFPPENTYQKEEEK
                     LPQLPTFPNNSIGNKFSQNTIKDAVTGRKEDEEVFEAEDFLEQHRGTMQKPPIKKSFS
                     KEYDEYEQSVSASKPEKKFSKEYDEDETFSAEEAMYERSIPAIPNITAETKVKEEPVK
                     EYAVRNYMTRKAEPVFIRIDKFEESMKIFQDIRSQISDIEKLIKNTKEIKAKEEEELT
                     SWEAEIQTVKNKIEKVNQEIFSRVE"
     gene            5285..5665
                     /locus_tag="PLU55_00700"
     CDS             5285..5665
                     /locus_tag="PLU55_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86612.1"
                     /translation="MKEQSIVHIKVDYEEALQSKRDILSSERDFLRIIKRIKRYKLLR
                     GEELNNRIKIQNKLKELNSNITKLNQTLPKVKLPNILKKKEEAKKKEIKEEKQSKIEI
                     KEIHEDDDLERQLIEIQEKLRKLG"
     gene            5693..7399
                     /locus_tag="PLU55_00705"
     CDS             5693..7399
                     /locus_tag="PLU55_00705"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPJ86613.1"
                     /translation="MNKMISREQLIKKYIDFFVSKNHKKIPNSSLIPENDPTALFISA
                     GMHPLVPFLLGEKHPLGKRLVGVQRCIRTTDIEEVGDEVHHTFFEMLGNWSLGDYWKE
                     ESIKYSLEFLTKELKISKEKLSVTCFKGDKDAPKDTESEKVWKSLGVNKIKFLPKESN
                     WWGPAGKTGPCGPDSEIFVNKVEIWNNVFMQYIKNEKQEYIQAKQKNVDTGMGVERTV
                     AILNNLKDNYLTECFLPIIKQIEQLSKKKYEDYKKEMRVIADHIKAAVFIINDGIVPS
                     NTEQGYILRRLIRRAIRYGQSINIKENFTHKLVDSIIKIYQDYPELKINRNKIIEELE
                     KEENRFRHTLERAINIFERITKDKKEISCKESFLLYQSYGFPVEMIEEECKSRKIKFS
                     KEEFENECEIHRELSRTAAEGKFKSGLADHSQETTKLHTASHLLLAAIKKIFKDDSIY
                     QKGSNITPERLRFDFNFPRKLTNEELKLIEDEVNKNIKKGCEVIREEMSLEEAKNKGI
                     SGVFEHKYGEIVSVYTIAGCSKEICTGPHVKNTCELGKFKIIKEESSSSGVRRIKAIL
                     EQ"
     gene            complement(7403..8509)
                     /locus_tag="PLU55_00710"
     CDS             complement(7403..8509)
                     /locus_tag="PLU55_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86614.1"
                     /translation="MGESKSKKRFFSFNYKSIFEKKYWVWTVFVFSLFIFFSGGFGII
                     ALIANDSHNPNSNKGYCGDDTVINGCSEVKPYFCLSEKLIELPFLCGCPDSFSSFNGT
                     CVSKHHTNPKTIRLKYFLNGKEDFIDFVAYEGFANYVSKIPRSIKYTNGYEFSRVDFK
                     LKAINDEEQRKFLLPLVIQIQNITDNKDDQAKIAISLVQNIPFGTSNKTTNFFGEVIN
                     HSRYPYEVLYDFQGVCGEKSDLLSFLLRELGYGVSFFYYHLENHEAIGIKCPIEESLM
                     ETGYCFVETTSPSLITDSQISYIGVGKLYSTPEIHIISDGLSLGRNLKEYKDADKLVK
                     IRNSIDKSGLLGPFNRKVLNNLKSKYGLTEMYYR"
     gene            complement(8555..11008)
                     /gene="leuS"
                     /locus_tag="PLU55_00715"
     CDS             complement(8555..11008)
                     /gene="leuS"
                     /locus_tag="PLU55_00715"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPJ86615.1"
                     /translation="MQETSGINFQKIEEKWQKKWQEKKIFEANRDDKKKKFYVLEMFP
                     YPSGSGLHVGHAFNYIIGDIFARFKLMQGFNVLHPIGYDALGLPAENAAIKDKIHPEE
                     YTNASIKNFMNQQKKLGLTYDWTRVINTANPEYYKWDQWIFLKMFEKGLVYEKEAPIN
                     WCPECGTIISNEQAQGGECERCGSKIEIRNMKQWFLKITDYADRLLEGHKKLNWPERT
                     IAMQKNWIGKSYGTEIDFEINGKKWPVFTTRPDTLFGVTFMVVSANHSKLNELISEKQ
                     KKDVEKFLKKLKSVSEKDLTDMEKEGVFTGSYAINPITKDKVPVYAGNFVVADYGSGM
                     VMAVPAHDQRDFEFAKKYKIKIKQVIEGKITKERAYTESGKLINSGNFDGFSNEEAKT
                     KITDYLITKNLARRVTNFKLRDWSVARQRYWGTPIPLLHCKKCGVVPVDEKDLPVVLP
                     KKVKFGKGNPLTTNEEWLNVKCPRCRGNARREANTLDTFVNSSWYFLRYCDPNNKKEI
                     FNKEDVNFWMPVDLYVGGAEHACMHLIYCRFYTMFLHDIGLIKFEEPVPRLFNQGMIN
                     DEYGEKMSKSKGNSVEPLKVMEEYGVDTTRFFMMSEASPDKGFNWSDKGIQGSLRFVN
                     KIFDTFEKLKFGKDSQDFLIKLNSAIKNISIQIEALDYRKSTIELRQLFDLIANQKEI
                     SKESFGKSLKLLSPFCPHITEELWEKLGHKNFISVSEWPKFDEAVFKFKTKEVDLNEK
                     YIKYIKELIEKFELNGTSVKKVFVYVVPFEIEKLNKSKLIKGIGKELDIFSVRDEKKY
                     DPENKSKKAIPGIPGIYFE"
     gene            11061..11579
                     /gene="endA"
                     /locus_tag="PLU55_00720"
     CDS             11061..11579
                     /gene="endA"
                     /locus_tag="PLU55_00720"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HPJ86616.1"
                     /translation="MKKITAYLVGEIVSTNEAEAFTLYKKSNFGQPTGQKVNYSLPET
                     LYLVEKNKIEIKKNKKSISFEELFRIFKKIDNRIQLKYPVFKDLRNKGYVVKTALKFG
                     SDFRVYEKGKTPDEEHAKWIVFCEHESKKFSWHEFSAKNRVAHSTKKNLLIAIIDEES
                     DVSYYEISWIKT"
     gene            11623..12711
                     /locus_tag="PLU55_00725"
     CDS             11623..12711
                     /locus_tag="PLU55_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86617.1"
                     /translation="MPTQDVSKIKERILSILRMKGPTLPVYISQEIGISMLFVSAFLS
                     ELSSDKLVKMSHMRVGNSPIYYIAGQEPQLEKFSNYLKSKEKEAFLLLQEKKFLRDSE
                     QPPAIRVALREIKDFAFPFRRNNETIWRYLTVKESEFKYDIKPIEEHQYVEAKPRVYN
                     QKERELGEKIVKDINIEDIEKIRKEKEELEKKKEVEKEEQKEIEDKVEEEIEEKPKIS
                     EEKEEKKDTAKEKKKEKTSKEEKQKTKEKSKPQKPSKKKAVQKQDDKFFDKIKEFLSK
                     ETIEILNVESFNKNEIHLRIKADGEEKLLVAHNKKRVAETDIINANKKASQLNLKYVI
                     LSMGEPTKKLSNLIEAAQNLDKIEKIEK"
     gene            12774..12974
                     /locus_tag="PLU55_00730"
     CDS             12774..12974
                     /locus_tag="PLU55_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17e"
                     /protein_id="HPJ86618.1"
                     /translation="MGKIKSKTVRKTANILIKEDLEFSKDFEKNKRILGKEMPSKRIR
                     NRLAGLISRLKKQEEKAKLEMQ"
     gene            13063..13183
                     /locus_tag="PLU55_00735"
     tRNA            join(13063..13100,13148..13183)
                     /locus_tag="PLU55_00735"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13097..13099,aa:Met,seq:cat)
     gene            13257..13634
                     /locus_tag="PLU55_00740"
     CDS             13257..13634
                     /locus_tag="PLU55_00740"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPJ86619.1"
                     /translation="MSNLFSKTRIFYISMTLIIALEIFLFSSIETPLGEQSGLNLATL
                     YHLAIFFAFTFFLTLSIKTKNLNKKTIVIIFLISLIYAISDEFHQLFVIGRFASIKDV
                     VIDMIGSSLAILILKILEIFKKI"
     gene            13772..15997
                     /locus_tag="PLU55_00745"
     CDS             13772..15997
                     /locus_tag="PLU55_00745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPJ86620.1"
                     /translation="MDEKEMQVFKDSKICFLSNFPPKECGIATFTQDLVNAMNKRFNP
                     KVKSRVIALNDESSIYNYDSHVIFEMDKDYLEDYINMAKRINRSDKIKIVCIQHEFGI
                     FGGEYGSYIIPFLETIEKPVVVTFHSVLPQPDNLRKNVVKFICDKCAATIVMAESAID
                     ILHDDYGVPKNKIHVIRHGIPDTPLKDTGPYKKKLRLENKIVLSTFGLLSRGKGIEYM
                     IKALPYLVKKYPNLLYIVIGETHPVVRKREGEEYRNYLTNLVKKLDLQNNVKFYNKYL
                     DLSEIIEYLLATDVYIMTNLERNQIVSGTLSYAMGCGKAVVSTPNLYAQEVLNNGRGM
                     IAKLQSPKSYADCVDKILSNPELKRNIEQSAYAYARSMIWSNVASGYLHLFNKVVKLR
                     EETTEKYPKIKLNHLKNLTDNIGCIQFSKLSVPDPSSGYTVDDNARALIVSTIHDRLY
                     NSEISKELSKTYLNFLERVQNEEGRFNDIEYENKNLVETSSDAIGRALWALGHLTNNS
                     SSPELISQAKKIFDKSYQLIETSSSPRTKAFTIIGLYHYYKKYNDEKILDKIKELVDS
                     LIESYKKEATEEWNWFESYLTYSNSKMPEALFLAYNLTKNPEYLEIAEKSLKFLTNLV
                     FFKDELSPIGQNGWYNRNGVRSFFDQQPIDASSMVQTYLTAYAITRKKSYYHNAVLAF
                     NWFLGKNHLKQMIYNDVTGGCHDGLSKSSINLNQGAESTIEYLISRLMLEEIKKRLPI
                     V"
     gene            complement(16003..16857)
                     /locus_tag="PLU55_00750"
     CDS             complement(16003..16857)
                     /locus_tag="PLU55_00750"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86621.1"
                     /translation="MKVKKFMAPVRIDFAGGTTDISPFKDEYGGCILNATINRYVVGE
                     LKADDKKTHLEYTGNIPTSSGLGTSGVMNLVWLSLITQQKGKLDSNAKIKLAETVYGI
                     EQAMGLVGGKQDQYAAAFGGINFMEFKKDKVKVHRLNLRKEFIKKLEDKLVLIYTGKP
                     HFSGNTNKAMMDNLRKGKNNANLLRIKNIAIEMKNALLKEDLERFAELMNEETRERKK
                     LHESIVPESTQKIISLAMKNGAIAAKVCGSGGGGSILFFGDKKKLKQKFKDKVIDFKF
                     DFEGLRAI"
     gene            complement(16873..17691)
                     /locus_tag="PLU55_00755"
     CDS             complement(16873..17691)
                     /locus_tag="PLU55_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPJ86622.1"
                     /translation="MAGKVIAVDLGGTNLRVSLVENNKVVKYIKNRTPKTQKELENLL
                     FKGVEELMEKDVKGIGVASAGPLEKGIIKNPPNLPLRNYNIKAALEKRFKKRVEVCND
                     AHSVAFAELKLGVKKKNFIVLTIGTGVGGGIIIDGKLYEGRGYGGEMGHIILDDGKSF
                     EKLVGWKYTKELSKKAFGKELIVSDLIKLNNPKSKAILNQITKYLGQGIGSLINVFDP
                     EVVVLAGGVKETGQVYLNMIKKEAEKYVILPRKTPIQWSKLDHPGVLGASLLIN"
     gene            complement(17681..18022)
                     /locus_tag="PLU55_00760"
     CDS             complement(17681..18022)
                     /locus_tag="PLU55_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HPJ86623.1"
                     /translation="MMKGVKIVKRPWGMFKQFVLNEKCTVKILEVKKNEELSLQKHKH
                     RSENWYFITPGYVQLGKKVKKVKEGELIKVGKNVAHRIIAKGSDVKVLEISFGVFNEN
                     DEIRLEDKYGR"
     gene            complement(18121..19914)
                     /locus_tag="PLU55_00765"
     CDS             complement(18121..19914)
                     /locus_tag="PLU55_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ86624.1"
                     /translation="MTHPIINAIKTEWGYLGKRRKWFIFYIFLFLIAGIVSLLTPYVI
                     GTIFNSIQESITTHEELVVLMKKIALLLFITVVFWMLHGPARFLETITGFHVKKNYVN
                     DKIRMVLKLPVKWHKDNHSGDTIDKINRASNALEEFSSHMTHDILYGLISFFGSIIIL
                     FFIDWRIGLFAFVYALIILFIISRVDKNLERKYKKLNEYNNKLSATIFDYLSNVITVI
                     TLRLRKTVSKEIESRQIASYKTFKSSSIINEIKWGFASVAIQIMIVISLIYKSYTEFN
                     LTGTILIGTLYMVYGYLNNVGQTFYKFAWLYGDVVRANARIIGANPLDEEYNKLEFPE
                     SGRLPRGWKEIKFKNLSFTYDTDGKRNHLDNVSFKLKKKEKIALVGESGSGKSTILSL
                     IRGLYKVEKGEIYCDGIPLEGGINRVKEQVTLIPQEPEIFNNTFRYNITMDLPASEED
                     LKKVIKIARLNPVLSKLPKGLDTSVQEKGVSLSGGEKQRLALARGLLASKKSEIILMD
                     EPTSSVDSMNEMKIHEQVFEEFQNKTIVSSIHRLHLLDKFDYIYLFDQGKIVGHGTFD
                     EIKKNPLFEKMWRRYHREKTSNKEKEEEKKR"
     gene            20065..20517
                     /locus_tag="PLU55_00770"
     CDS             20065..20517
                     /locus_tag="PLU55_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPJ86625.1"
                     /translation="MVKKQNKKANKKEDILLNIFHEEQDKKGYISIDFLKKISTKYNI
                     PISRLYGVVKFYTMLRTEPQGKYIIELCGSPTCVLHESREIENFLKKELKIDIGDTTK
                     DKMFSVYKTSCIGCCDEPPAMLLNGKPITNLTIEKVKKLIKELKSKKK"
     gene            20520..21656
                     /locus_tag="PLU55_00775"
     CDS             20520..21656
                     /locus_tag="PLU55_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPJ86626.1"
                     /translation="MQILKKTNLQALKLAKKLGPEKVLQILERKYLVGRGGAGFPLAK
                     KWKLALDAKKKNPSKEIFIICNADEGEPGTFKDKFILKNNPETLIEGMLIASYVLNTK
                     KAFIYLRNEYEHLLKDLRKTISSVLKKSKMENKVKIEIVRGAGAYICGEETAIMQSIM
                     GYRGQSQYKPPYPTEQGLWESPTVLNNVETLTCVPQALLYNDWNPNLRLFSTSGDIEK
                     PGIYEWELGVKMSTILETVKPTQEIKAISFGCFGGIMPINKNTLVTAETICEKDCQHG
                     AFTLIFIGKNRNIVDICLSIAKFYTYESCGKCTPCREGTIRILNLLKKVRDKKATQSD
                     LDLLKELSYHIKETSLCGLGQSSTNHILTSLQHFKEDYNYYLKK"
     gene            21658..>23301
                     /locus_tag="PLU55_00780"
     CDS             21658..>23301
                     /locus_tag="PLU55_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HPJ86627.1"
                     /translation="MKITLNNKQIKIDPNKTILQICKENNIKIPTLCYHKNLFPEARC
                     RVCLVEMNGKLVTSCSTKPVENATIITNSEKVIKARKMNLELMNQNIKLNELSDDFEA
                     REVYDEIGIENNRFQSLKDYYPDLGAAIVRDNNKCVNCGKCVQVCGKIQEIHAIDFAS
                     RAHNEHVTPYGEKKLEQVACIQCGQCLINCPVGAIIERTHLSEVLEALKNPKKHVVAQ
                     TAPSIRAALGELFGMPAGTLVTGKMVASLRRCGFDKVFDVDLGADMTIMEESAELLKR
                     LKEKNKKKLPMITTCCPAWILMMESFYPELIPNMSTCKSPHEMLGMLVKSYYAELANI
                     NPKDIVVVSIMPCTAKKFESTRSELNTGVDYVLTTRELGRLIKMKNIDFVNLPDEDFD
                     PTLGLASGAGDIFGAAGGVMEAALRTAYEISTNPPKKLEKVEFKNLRTDDSSIREGTI
                     KLGNKTVRYATTSGGANIRKLLKNKDKYDFIEMMACPGGCIGGGGQPIYSDDKVLTLR
                     AQALYKQDNMRKYRRSHENPIVKDIYKKYLGKPLSRKAEK"
BASE COUNT         8883 a         3863 c         3053 g         7502 t
ORIGIN      
        1 ctgcagattt atgccttgtt cctgtttctc ttgtagttat ttctgaattt ggtgaaagaa
       61 aagaatttgc atagagtatt ttctttacgt tttttgaaag aattattgct ccatccatct
      121 tagctaattc aactagtttt tggggtgtga atttagaatg aataccaaaa cctttttgat
      181 aagcagaaga aatatttcca ttatctataa caattaatgc tcccatacct gcattaagca
      241 aatcatccat tgctactctg atagatgttc ctggtgcaac catcttaaga acatccataa
      301 aatctttttc aacaactgtt ttttcctgtt caccctgcgg gataaacaaa tcacctattt
      361 ctttattctc ctccataaaa aatggataaa aaacgtatat ataagctttt atatagaatt
      421 aaacaaaata tattaactat accatttagg ggctacaaat cttgaaagag cacatcttgt
      481 agcatcttca aactctttaa ctctagtata taacccacct gtcaaaattc cattagttcc
      541 ttcattatgt cttatagaat ttttttcatc attcattaat tcctgtatta ttggacctaa
      601 ttcctctcct ttttttattc tttgttctat tgtctttggt atatgcacgc ttgcactctg
      661 tccaaaacca atattcccat atttatccac aatagtaacc catccatgaa gaaacatccc
      721 ataattattt gtgtttacgg tcccttcaag cccaatacca taatctgcat ctcctttgtt
      781 aatagcttcc tttgctctat taattgctcc tttaattgcc tcttcatcac taagtggctg
      841 atctttaaca agtgaatttg ttttatgata tgaaaactca acatccttcc aaacttcctt
      901 tataatactc tctactgcct ttatcttagc tttattttca gttcctattg caactttcat
      961 atacaataaa aatttgactt ctcttaaata ttttattgta taacattata gcaattacaa
     1021 caattaattt ttcataactt tactaaatta gaaactcaat tatttttagc aaatgttttt
     1081 gcaacaagta aaggcaacaa aattgttgcg tctccataaa ccttaactct tttacattca
     1141 ggcgttaatt taccccaaga aacagcttct tcaggaagag ctccagcatc acttccatca
     1201 aattcctgtg cattgtttat ataaacagca taatctgcac catttctata caaatgagca
     1261 ttaagaatag catgtttaac aatccctgct cccacaatca acactccaga tttttcaata
     1321 cccatagtag attcatttag ttgccttgtc tcttctgcaa tatcaacttt aaaatcttca
     1381 tgtttatact taaagaaata aaccatatct ccaaaacttc catctaagat tgctggacaa
     1441 taaataggaa tcttattttt ccaagcccaa taacaaatac tttttttatt gtttattttt
     1501 tctccaaatt tccaaattat ttcataagga gtgtaaacct ttcctgtttt cttttgctct
     1561 tgatacattt cttcaagaat tggcattgaa aactcctcaa attttatata attattatta
     1621 ggtacaagta aatttcctat cctgtttatt gctttctctc taagttcttt acctggcaat
     1681 ctaaaatctc ctaaaacagt ttctccaaga cattttatta tatcctcttc aaaaccccct
     1741 ccgcttgtaa cacaaacatc tactttttta tgctcaacta aatatcttat tacttctctc
     1801 aaaccagaac taactatatt tgaagtatat cccaaataaa ttttagattt aatcttaatc
     1861 atctcattaa ctatatctat ggcctctcct aaatttgaag cctgaaaacc agtattagaa
     1921 aaactatcaa ttattttgtt gtaatcaact cctttattaa aatcataacc ttctataatc
     1981 ttaccttcaa ctttatctga ttttttaagt atgtttttag ctacatctga ttcttgggat
     2041 ttaatggatt ttttcattaa atacaataat taaattaact taaaaatcta tctttgacta
     2101 tcaaaatctt tttaaagaaa aaccccatag ttaaaataca atgaataagt ggttagaatt
     2161 aatcttggga gttattcttt tagtaggagt tgtagcttta gtgtttccag gtatgcctat
     2221 gcaatcttgg ggatatgcag cttggacagt cctaaaaggc ggattaactt ggatagtcgc
     2281 aataacaggt ttagttctta taattcttgg aataagcgaa ataaagggat aaaaatattc
     2341 agcctaaaaa atgaataaag gttccttcaa ttattgctgc aattattaaa agaggaacta
     2401 ctacaaaaat aaatactctt aaagattcaa caaagaataa actaaaattt ttcatctttt
     2461 ctttataaaa ccaaaaagtt ccaaacttaa atcctaatcc aaatgagata aatatagcag
     2521 gtaattcaaa aataccatgt ggaactaaac ttaaaagaga acttactcct gaagaagaaa
     2581 ctgcaaaaaa agcgacaaaa ccaacaacat atccattaac caaagagaat aaaactgagg
     2641 gtataccaaa taaaaaacca tagagcaccc ccataaaacc aactttaaaa ttattaaaaa
     2701 agataaatga ggttaattca ggataagaca tatcttctgt cttatttaag agttcattta
     2761 ccatctccat aattaattgg gaaatacttt ctggctgaaa tataaaacca attgcaacaa
     2821 aagaaaaaaa tattataaca atggcccaaa taaattttct agattcaaca agataatccc
     2881 aacatttttt ataattttca gagaaaaaat tacgtttaat ttttttattt accctccttt
     2941 ttttcataaa aaaaacaaac aataattcta tataaaacta actaaaatac cttcttatat
     3001 aagctgtttt atttctatat tttgatttta aaaatagccc ataaattaat cctgcaatca
     3061 atcctcctaa gtgagctgta ttccctatag aaacatttcc tgctagagaa ataacccata
     3121 gtaatataag catacctgga gcagcatatt tcatttttac aggaatagga ataaacatta
     3181 tgtaaacggg aagattagga gttaacagca tcaacagacc aatcaaacca aataatgctc
     3241 cagaagcccc tacagcataa gaatataaat ctactttaaa caaaaagcta actaagataa
     3301 aaaatgctcc tgcaaatatt cctgagaaaa tataaaacca aaaatatctt cttctgccaa
     3361 ttatcttttc tataagagaa cctacaaaga ataatgaaag catattaacc aacaaatgga
     3421 aaaatcctgc gtgcataaac atgcttgtta aaaatgtcca caaatacttg ccatccaaaa
     3481 tatttgaagg cattatagca atatcatcaa cagaaagaaa atttaacttt attaaaacac
     3541 taaaaataat aaataaaaca acattcacga ttatcaacaa ggtgttcaaa ctaaaagaat
     3601 cgaaaaaatt tttctttttc tttggattaa ttctatagac gtaggtcatt ttaagaggaa
     3661 tctaatttac tttttcttag attttttttt atcttttact tttgaaggtt ttttaatatt
     3721 ctttttaggt ttagatggag tagaagattt tttctttgaa gattttttct ctgagggctt
     3781 tttctttgag gattcttttt taagtttctc tctagcttct ttctgaattc ttcttttctc
     3841 ttccctttgt ttaagttttt cagcttcaat cttttttagt tctttcattt taattttatc
     3901 caacctagct ttttcagttg taatctcctc ctcaattaac ttaagcttat ttaccaattt
     3961 atcctcattc tttttaaact ccctaataac cttttcatta tcttttacag taaaaacttc
     4021 accacattca ttacaagtaa aatcatgcaa catagcattt tcctcattaa attctatatt
     4081 gcacctctcg caaacataaa atcctttagt ttctctgctc tttatctggt ttcttaactg
     4141 agtaatattt tttattgtga gttcttttaa aaattcaaga gatttaagca cttcaatttt
     4201 ccaaaaataa gtataccatc cttttttctt atcttttttc cttattgaag aaacaagccc
     4261 ataatcagaa atcttatata aaatatttct agtttgattt atagttaaat ctagcttttt
     4321 tgcaattata aactcattca catatttatc cccttccaat aatcttacaa ttgaatcaga
     4381 tggtttacca acgacaatag aaattacctc atttaaaaaa ttctttaaca tcaaaataat
     4441 aaaaatagtt tgattatata tttttcgttt tattaaaaaa ataaaaaaga taaaagtttt
     4501 taaacctata ttgtatatta tatgtattaa aaagagatat atcaaacaag acaaaaatgg
     4561 gtttattcgg aaaaaaagat aaaaaagagg agataaaaag acctgttcag tcggttcctg
     4621 aactgccgga acttccaaag cttccagagt ttcctcctga aaatacttat caaaaagaag
     4681 aagagaaatt gcctcaactc ccaacattcc caaataattc tataggaaat aaattttcac
     4741 aaaatactat taaagatgcg gtaactggga gaaaagagga tgaagaggtt ttcgaagcag
     4801 aggacttcct tgaacaacat agagggacga tgcaaaaacc tccaatcaaa aaatcattca
     4861 gtaaagaata tgatgaatat gagcaaagtg taagtgcatc aaagccagaa aagaaattca
     4921 gcaaagaata tgatgaagat gaaacttttt cagcagagga agcaatgtat gaaagaagca
     4981 tccctgcaat accaaatatt actgctgaaa caaaagtaaa agaagaacca gttaaagaat
     5041 acgctgtaag aaattacatg actagaaaag ctgaacctgt tttcataaga attgataaat
     5101 ttgaagaaag tatgaagata ttccaagata ttcgatcgca aatatcggat atagaaaagc
     5161 tcatcaagaa tacaaaagaa ataaaggcaa aagaggaaga ggagcttact tcttgggagg
     5221 ctgagataca aacagtaaaa aataaaatag aaaaagtcaa ccaagaaatt ttctcaagag
     5281 tagaatgaaa gaacaatcta ttgtacatat aaaggttgat tacgaagaag cactgcaatc
     5341 aaaaagagat atactctctt ctgaaagaga tttcttaaga ataataaaaa gaataaaaag
     5401 atataaatta ctaagaggag aagaattaaa caatagaata aaaatacaaa ataaattaaa
     5461 agaattaaac tcaaacataa ctaaattaaa tcaaacgctc ccaaaagtta aattaccaaa
     5521 tatattaaag aaaaaagaag aagctaaaaa aaaggagatt aaagaagaaa aacaatcaaa
     5581 gatagagata aaggaaattc atgaagatga cgatttagaa agacaattaa ttgaaataca
     5641 ggaaaagctt agaaaacttg gataagcaat attttaaaaa taacctaatt ctttgaataa
     5701 gatgatatcc cgagaacaat taataaaaaa atatattgat ttctttgttt caaaaaatca
     5761 taaaaaaata cctaattcca gtcttattcc agaaaatgat cctacagcat tgtttatctc
     5821 tgctggaatg caccctctag tccctttttt attaggggag aaacatcctt taggtaaaag
     5881 acttgtagga gttcaaagat gtataagaac aactgatatt gaagaggttg gagatgaagt
     5941 tcatcacact ttttttgaga tgttgggcaa ctggagttta ggggactatt ggaaagaaga
     6001 atcaataaaa tacagtctag aatttcttac aaaagaactt aaaatatcaa aagaaaaatt
     6061 atctgtaact tgctttaagg gagataaaga tgctccaaaa gacacagaat ctgaaaaagt
     6121 atggaaatca ttaggagtaa acaaaataaa attcttgcct aaagaaagca attggtgggg
     6181 acctgcagga aaaacaggtc catgtggacc tgatagtgag atatttgtaa ataaagttga
     6241 aatatggaac aatgtattta tgcagtatat taaaaatgaa aaacaagaat acattcaagc
     6301 aaaacaaaaa aatgttgata caggcatggg cgttgaaaga acagttgcta tattaaacaa
     6361 cttaaaagat aactacctca cagaatgctt tcttccaata ataaaacaga ttgaacagtt
     6421 atctaagaaa aaatatgagg actataaaaa agaaatgagg gttatagctg accacataaa
     6481 agcagctgtt tttataataa atgatggaat tgttccaagc aacacagaac aaggatacat
     6541 tctaagaagg ttaataagaa gagctatacg ttatggacag tcaataaaca taaaagaaaa
     6601 cttcacacat aaactcgttg attcaattat aaaaatatac caagattatc ctgaattaaa
     6661 aataaataga aacaagataa ttgaagaatt agaaaaagaa gaaaatagat ttagacacac
     6721 tttagaaaga gcaataaaca tattcgaaag aataactaaa gacaaaaaag aaatatcttg
     6781 taaagaatca tttttattat accaatctta tggctttcct gttgagatga ttgaagaaga
     6841 atgcaaatct agaaaaataa aattctcaaa agaagaattt gaaaatgaat gtgagataca
     6901 tagggaactt tcaagaacag ctgcagaagg aaagtttaaa tcaggtttag cagaccattc
     6961 ccaagaaact acaaagttac acactgcatc acatctacta ttagctgcta taaaaaaaat
     7021 ctttaaagat gactcaatct atcaaaaagg ttcaaacatc actccagaaa gattaagatt
     7081 tgattttaat tttccaagaa agctaacaaa tgaagaattg aaattaatag aagatgaagt
     7141 aaacaaaaat ataaaaaaag gatgtgaagt aataagagaa gaaatgtctt tagaagaagc
     7201 aaaaaacaag ggaataagtg gagtatttga acacaaatat ggagaaattg tttcggtcta
     7261 taccattgca ggatgttcta aagaaatatg tacaggacca catgtaaaaa atacttgcga
     7321 attaggaaaa ttcaaaataa taaaagaaga atcaagtagt tctggggtta gaagaattaa
     7381 agcaatacta gaacaataat tattatctat aatacatttc agttaatcca tattttgatt
     7441 ttaaattatt gagaaccttt ctattaaaag gtcccaacaa tccactttta tctattgaat
     7501 ttctaatttt tactaattta tctgcatctt tatattcttt aagatttctt cctaaagata
     7561 atccatctga aataatatgt atttcaggag ttgaatataa ttttcctact ccaatataag
     7621 aaatttggct gtcagtaatt aaagaagggg aagttgtttc tacaaaacaa tatcctgttt
     7681 ccattaagct ctcttcaata gggcatttta tccctattgc ttcatgattt tccaaatgat
     7741 aataaaagaa agaaacccca taccctaatt ccctaagcaa aaaagaaagc aaatctgatt
     7801 tttcgccaca tactccctga aaatcataaa gaacttcata tggatatctg ctatgattaa
     7861 ttacttctcc aaaaaagtta gtagttttat tagaagttcc aaaaggaata ttttgaacaa
     7921 gacttattgc aatttttgct tgatcgtctt tattatcagt tatattttgt atttgaatta
     7981 ctaaaggcaa aagaaacttc ctttgttctt cgtcattaat agctttaagt ttaaaatcca
     8041 cccttgaaaa ctcgtatcca ttagtatact taatagagcg aggtattttt gagacataat
     8101 tagcaaaacc ttcatatgct acaaaatcaa taaaatcctc ttttccatta agaaaatact
     8161 ttaagcgaat tgtcttggga ttagtatggt gtttagatac acaagtccca ttaaaagaac
     8221 taaatgaatc tggacatcca caaaggaatg ggagttcaat taatttttct gataaacaaa
     8281 aataaggctt aacttctgaa caaccattaa taactgtatc atctccgcaa taacctttgt
     8341 ttgaatttgg attatgggaa tcattagcaa ttagtgcaat aatcccaaaa cctccagaga
     8401 aaaagataaa caagctaaat acaaaaacag tccagaccca gtattttttt tcaaaaatag
     8461 atttataatt aaaagagaaa aaacgttttt tagatttaga ttcacccata atataaatag
     8521 aaaaagtgca tataaaaacc tgattatttt aatttcactc aaaataaatc cctggaattc
     8581 ctggaattgc ctttttggac ttattttctg ggtcatattt cttctcatcc ctaacagaaa
     8641 atatgtccaa ttcttttcca atacctttaa tcaacttgga tttatttagt ttttcaatct
     8701 caaatggaac aacataaaca aaaactttct taacagaagt tccatttaac tcaaattttt
     8761 caattaattc tttaatatat ttaatatatt tttcattcaa atcaacttct tttgttttaa
     8821 atttgaaaac tgcttcatca aactttggcc attctgaaac agaaataaaa ttcttatgtc
     8881 ccagcttctc ccataactct tctgtaatat gtggacaaaa aggagaaaga agcttaagtg
     8941 atttaccaaa agactctttt gaaatttctt tttgatttgc aattaaatca aataattgcc
     9001 ttaattctat tgtagatttt ctataatcaa gtgcttctat ttgtatacta atatttttaa
     9061 tagcactgtt taatttaatt aaaaaatctt gagagtcttt tccaaatttc aatttttcaa
     9121 aagtatcaaa tattttatta acaaatctta aactcccttg aattccctta tcactccaat
     9181 taaatccttt atctggagaa gcctcagaca tcataaaaaa tcttgtagta tcaacaccat
     9241 attcttccat tacttttaat ggttctactg aattcccttt tgattttgac atcttttcac
     9301 catactcatc attaatcata ccctgattaa acaatctagg aacaggttct tcaaatttaa
     9361 ttaatccaat atcatgaagg aacatggtat aaaatctgca ataaattaaa tgcatacatg
     9421 catgttctgc accaccaaca tacaaatcaa caggcatcca aaaattaaca tcctctttat
     9481 tgaaaatctc ttttttatta ttagggtcac aatatcttaa aaaataccaa gaagaattca
     9541 caaaggtatc taaagtattt gcttctcttc ttgcattacc tctgcatcta ggacatttta
     9601 cattcaacca ttcttcattt gttgttaaag gatttccttt tccaaattta acttttttag
     9661 gaagaacaac aggcaaatct ttctcatcta ctggaacaac tccacacttt ttacaatgta
     9721 acaagggaat aggagttccc caatatctct gcctagcaac actccaatct ctaagcttaa
     9781 aattagtcac tcttcttgca agatttttag ttatcaaata atctgttatt tttgtctttg
     9841 cttcttcatt agaaaagcca tcaaaatttc ctgaattaat tagcttccca gattcagtat
     9901 aagctctttc ttttgtaatc tttccttcaa ttacttgttt tattttaatc ttatattttt
     9961 ttgcaaattc aaaatctctt tggtcatgtg caggaacagc cataaccatt ccagaaccat
    10021 aatctgcaac cacaaaatta cctgcataaa ctggaacctt gtctttggta ataggattta
    10081 tagcatagct tcctgtaaat acaccttctt tttccatgtc agttaaatct ttttcagaaa
    10141 ctgattttaa ttttttaagg aacttttcaa catctttctt ttgtttttca gaaataagct
    10201 cattaagttt tgaatggttc gcggaaacaa ccataaatgt aactccaaaa agagtatctg
    10261 gacgagtagt aaatacaggc cactttttcc cattaatttc aaaatcaatt tcagtcccat
    10321 aagattttcc aatccaattt ttctgcattg caattgtcct ctcaggccag tttaattttt
    10381 tatgtccttc taataatcta tctgcataat ctgtaatttt caaaaaccat tgtttcatgt
    10441 ttcttatttc tatttttgaa ccacatcttt cgcattctcc tccttgagct tgttcattcg
    10501 aaataattgt accacattct ggacaccaat taataggagc ctctttttca tacaccaatc
    10561 ctttttcaaa cattttaaga aaaatccatt gatcccactt gtagtactct ggatttgcag
    10621 tattaataac tcttgtccaa tcataagtta aaccgagttt tttctgttga ttcataaaat
    10681 tttttataga agcattagta tactcttctg gatgaatttt atcttttatt gcagcattct
    10741 cagcaggaag tccaagagca tcatatccta ttggatgaag aacattaaaa ccttgcatta
    10801 gcttaaatct tgcaaaaata tctccaataa tataattaaa tgcatgtcct acatgcaatc
    10861 cagacccaga aggataagga aacatctcaa gaacataaaa tttctttttt ttatcgtctc
    10921 tattagcttc aaatatcttc ttttcctgcc actttttctg ccatttttct tcaatcttct
    10981 gaaagttaat tccactagtt tcttgcataa attacctact attcaggaac ttttaaatat
    11041 tctcctaaca taaaccaatt atgaaaaaaa taactgctta cctagtgggt gaaatagttt
    11101 caacaaacga agcagaggca tttactcttt acaaaaaatc taattttgga caaccaacag
    11161 gacaaaaagt aaattactcc ctaccagaaa cattatatct agttgaaaaa aacaagatag
    11221 aaataaaaaa gaacaagaaa tcaataagtt ttgaagagct tttcagaata ttcaaaaaaa
    11281 tagataatag aattcaatta aaatatcctg tgttcaaaga cctgcgtaat aaagggtatg
    11341 ttgttaaaac tgctcttaaa ttcggttcag attttagagt atatgaaaaa ggtaaaaccc
    11401 cggatgaaga acatgctaaa tggattgttt tctgtgaaca cgaatccaaa aaattctctt
    11461 ggcatgaatt ttcagcaaaa aatagagttg cacactctac aaaaaagaac ctcttaattg
    11521 caataattga tgaggaatct gatgtgtctt attatgaaat aagttggatt aagacataat
    11581 tatcataaaa atctttataa tatgcagtat tctccataag ttatgccaac ccaagatgtt
    11641 tcaaaaataa aagagagaat cctttcaatc ttgagaatga aaggtccaac acttcctgta
    11701 tatatttctc aggaaatagg aataagtatg ctatttgtat ctgcattttt atcagaactt
    11761 tcatcagata aacttgtaaa aatgagccat atgcgtgtag gaaattctcc tatttattat
    11821 attgcaggac aagaacctca attagaaaaa ttttcaaatt atcttaaaag taaagaaaaa
    11881 gaagctttcc tgcttttaca agaaaagaaa tttttaagag attctgaaca accacctgca
    11941 ataagagttg cactaagaga aataaaggat tttgctttcc ccttcagaag aaacaatgaa
    12001 accatttgga gatatttaac agtaaaagaa tctgaattta aatatgatat aaaaccgata
    12061 gaagaacacc aatatgttga agctaaacca agggtatata atcaaaaaga aagggaactt
    12121 ggtgaaaaaa tagtaaaaga cataaacata gaagacatag aaaaaataag aaaagaaaaa
    12181 gaagaacttg aaaagaaaaa ggaagtagaa aaagaagaac aaaaagaaat tgaagataaa
    12241 gttgaagaag aaatagaaga aaaaccaaaa atatctgaag aaaaagagga gaaaaaggat
    12301 acagcaaagg aaaagaaaaa agagaaaacc tcaaaagaag aaaagcaaaa aacaaaagag
    12361 aaatcaaaac cccaaaaacc ttcaaaaaag aaagcagtac aaaaacaaga cgataaattc
    12421 tttgataaaa taaaagaatt cctttcaaaa gaaacaattg aaatactaaa tgtggaaagt
    12481 ttcaataaaa atgaaatcca tttaagaata aaagcagatg gtgaagaaaa actccttgta
    12541 gcccacaata aaaagagggt agcagagaca gacataatta atgcaaacaa aaaagcctct
    12601 caattaaacc ttaaatatgt aattttgagt atgggagaac caaccaaaaa actctcaaat
    12661 ttaattgaag cagctcaaaa cctagacaag atagaaaaaa tagaaaaata aatgaaaaaa
    12721 ttcaaaaatt tcttaatcaa aacctttaaa aacaaatttc tgcacaaatt aacatgggaa
    12781 aaataaagtc aaaaacagta agaaaaactg ctaacatctt aataaaagaa gatttggaat
    12841 ttagcaaaga ttttgaaaaa aacaaaagaa tattaggaaa agaaatgcct agtaaaagaa
    12901 taagaaacag gcttgcagga ctcatctcta ggctgaaaaa acaagaagaa aaggccaaac
    12961 ttgaaatgca ataaaatata aatccctgtt tttctaaagg ttaaacattt aaaagcacct
    13021 atttataata aagtatattg ctctgtagct cagtgttttg atggctctgt agctcagttg
    13081 tttagagcgc ggctctcatg aattaaatta gtccgacaaa tctgtatagg acttgtttga
    13141 ttgggtgagg ccgaggtcgg gagttagaat ctccccagag ccattttcta aaaataatct
    13201 aaacatatct tttaaattat caaagtgtat aaaaacaaaa acaagttctt ttcaaaatgt
    13261 caaatttgtt ttcaaaaacg agaatttttt atatttcaat gactctgata attgctttag
    13321 aaatattttt attttcaagt atagaaacgc ctcttggaga gcaatcaggc ctaaatctag
    13381 caacactcta tcatttagca atattctttg cattcacttt ctttttaact ctttcaataa
    13441 aaacaaagaa tctaaacaaa aaaacaattg tgattatctt tttaatttca ttaatctatg
    13501 caatctcaga tgaatttcat cagctgtttg tgattggaag atttgcgagc atcaaagatg
    13561 ttgtaattga tatgattgga agttcacttg caatcttaat tctcaagatt ctagagattt
    13621 ttaaaaaaat ataattatcg tcgataaaat aaaaaaacta tttaatctca atcttatctc
    13681 aattaaagaa caaataaaaa atcaaccaaa aagcgttgat tttataaagt ccaaagtttt
    13741 agttatagta tcgcaaaacg gattcgttaa aatggatgaa aaagagatgc aggtatttaa
    13801 ggactcaaaa atatgttttt taagtaattt cccgccgaaa gagtgtggta ttgcgacatt
    13861 cactcaagac ctagtaaatg caatgaataa gaggttcaat ccaaaagtta aatcaagagt
    13921 tatagcttta aacgatgagt caagtatcta caactatgac agccatgtaa ttttcgaaat
    13981 ggacaaggac tatcttgaag attatataaa catggcaaaa agaataaacc gttcagacaa
    14041 aataaaaatt gtttgcattc aacatgaatt tggaattttc ggaggagaat atggaagcta
    14101 tattattcca ttcttagaaa caatagaaaa accagttgtt gtaacatttc atagtgttct
    14161 tccacaacca gacaacctaa gaaaaaatgt tgttaagttc atctgtgata aatgcgctgc
    14221 aacaattgtc atggcagaga gtgctataga tattttacat gatgactatg gagttcctaa
    14281 aaacaaaatc catgtaatcc gtcatggtat cccagacact ccattaaaag atactgggcc
    14341 ttataaaaag aaattaagac ttgaaaacaa aatagttctt tcaactttcg gattattaag
    14401 tcgaggaaaa ggaattgaat atatgattaa agctcttcca tacctagtta aaaaatatcc
    14461 aaatctatta tacatagtaa taggagaaac gcatccagta gtgagaaaaa gagagggaga
    14521 agaatataga aattatttaa caaatcttgt taaaaagttg gacttacaaa ataatgtaaa
    14581 attttacaac aaatatcttg atttaagtga aataatagaa tatcttcttg caacagatgt
    14641 ctacataatg actaatcttg aaagaaatca aatagtttca ggaactcttt cttatgctat
    14701 gggatgtggt aaagctgttg tttctactcc taatctttat gctcaagaag ttttaaataa
    14761 tgggagagga atgattgcaa aacttcaaag cccaaaatct tatgcggatt gtgtagataa
    14821 aatactttca aatccagaat taaagagaaa tatagaacaa agcgcttatg cttatgcaag
    14881 gtctatgatt tggtcaaatg ttgcctcagg ttatttacat ttatttaata aagtcgtcaa
    14941 attaagagaa gagacaactg aaaaatatcc taagataaaa ctcaaccatc taaagaatct
    15001 tacagataat ataggatgta ttcaattttc aaaactttct gtaccagacc catcatcagg
    15061 ttatactgta gatgacaatg caagagcttt aattgtctca acaattcacg acagattata
    15121 taactctgaa atatcaaaag aactttcaaa gacatactta aacttcttag aaagagttca
    15181 aaatgaagaa ggaagattca atgacattga atacgaaaat aaaaatctag ttgaaacaag
    15241 ttcagatgca attggaagag cattatgggc attaggtcat ttaacaaata atagttcaag
    15301 tcccgaacta ataagccaag caaaaaaaat atttgacaaa tcctatcaat taatagaaac
    15361 aagtagttct ccaagaacaa aagcatttac aataattgga ttatatcact attacaaaaa
    15421 atacaatgac gaaaaaatat tagataaaat aaaagagcta gttgattctc ttatagagtc
    15481 ttataaaaaa gaagcaacag aagaatggaa ttggtttgaa agttacttaa cttattcaaa
    15541 ttccaagatg ccagaagctc ttttcttagc ttataactta acaaaaaatc cagaatatct
    15601 tgaaatagca gaaaaaagtt taaaattctt aacaaatttg gttttcttta aagatgaatt
    15661 gtctccaata ggacaaaacg gatggtataa tagaaatggg gtaaggagct tctttgacca
    15721 acaaccaata gacgcttctt caatggttca aacttatttg acagcatatg ctataacaag
    15781 aaaaaaaagc tattatcata atgctgtttt agcattcaat tggttcttag gcaaaaacca
    15841 tcttaaacaa atgatttaca atgatgttac aggaggatgc catgatggac tctctaagag
    15901 ttcaataaac cttaatcaag gagcagaatc aacaattgaa tacttaattt cgagattaat
    15961 gcttgaagaa ataaaaaagc gtttacctat tgtataaatc tattaaatag ctcttaatcc
    16021 ttcaaaatca aatttaaaat caataacttt atctttaaac ttctgtttta attttttctt
    16081 atctccaaaa aataaaatac tccctcctcc tccagaaccg cacactttag ctgcaattgc
    16141 cccatttttc atagctaagg aaattatctt ttgagtgctt tcaggaacta tactctcatg
    16201 taatttcttt ctttctcttg tttcttcatt cattaattca gcaaatcttt ctaaatcctc
    16261 tttcaataaa gcattcttca tttcaattgc tatattcttt attctaagta aatttgcatt
    16321 attctttcct tttcttagat tatccatcat agccttatta gtattgcctg aaaaatgagg
    16381 tttgcctgta taaattaaaa caagtttatc ttcaagtttt ttgataaatt cttttcttaa
    16441 atttaatcta tgaactttaa ctttatcttt tttaaactcc ataaaattaa ttccaccaaa
    16501 tgctgcagca tactggtctt gctttcctcc aactagtccc attgcctgtt caatcccata
    16561 aactgtttca gcaagtttaa ttttagcatt tgaatctaat ttaccttttt gttgcgttat
    16621 taaactcaac caaactaaat tcattactcc cgaagttcct aatccagaag aagtaggtat
    16681 attcccagta tattctaaat gagttttttt atcatctgct tttaattctc caacaacata
    16741 tctatttatt gtagcattaa gaatacatcc cccatattca tctttaaatg gagaaatgtc
    16801 agtagttcca ccagcaaaat caattcttac aggagccatg aattttttta ctttcatatt
    16861 cattaattta ttttaattta tcaacaaact tgcacccaat acacccggat gatcaagttt
    16921 actccattga ataggtgttt ttcttggcaa aataacatac ttttctgctt cttttttaat
    16981 catattcaaa taaacctgtc ctgtttcttt tactccccct gctaagacaa caacttcagg
    17041 atcaaaaaca ttaattaaac ttccaatacc ctgtccaaga tactttgtta tctgatttaa
    17101 aattgctttt gatttaggat tattcaattt aattaaatcg cttactatta attctttccc
    17161 aaatgctttt ttagacaatt ctttagtata tttccatcca actagttttt caaatgattt
    17221 accatcgtct aaaataatat gccccatttc tcctccatat cctctccctt catacaattt
    17281 tccatcaatt attattcctc ccccaactcc tgtgcctatt gtaagaacaa taaaattctt
    17341 tttcttaaca cccaacttca attcagcaaa tgcaacagaa tgagcatcgt tacacacttc
    17401 aactcttttc ttaaatcttt tttcaagagc agcttttata ttataatttc tcaaaggtaa
    17461 attaggagga ttttttatta ttcctttttc aagaggtcca gcacttgcaa ctcctatacc
    17521 cttaacatct ttttccatta gttcttcaac acctttaaac aatagatttt ccaattcttt
    17581 ttgagttttt ggagttctgt ttttaatata tttgacaacc ttattattct caactaatga
    17641 aactcttaaa ttagttccac ccaaatcaac tgcaataact ttacctgcca tacttgtcct
    17701 ccaatcttat ttcatcattc tcattaaaca caccaaatga aatttcaaga actttaacat
    17761 ctgaaccctt tgcaattatt ctatgggcaa cattctttcc aaccttaatt aattctcctt
    17821 ctttgacttt cttaactttt ttacctaatt gaacatatcc aggagtaata aaataccaat
    17881 tttcacttct gtgtttatgt ttttgtaaac taagctcttc atttttcttg acttcaagaa
    17941 tcttaacagt acatttctca ttaagaacaa attgcttgaa catgccccac ggtctcttaa
    18001 ctatcttcac ccctttcatc atttaaaaaa acaaatatgt tttaaatagt ttatgatgct
    18061 tcttcaaaat acttttttca gaacaattat ttgattgttg ttttaaagat tgtagaacct
    18121 tcatctcttt ttctcctctt ccttctcctt attagatgtc ttctccctat gataccttct
    18181 ccacatcttt tcaaacaaag gatttttctt tatttcatca aatgttccat gacctacaat
    18241 cttcccttgg tcaaataaat aaatataatc aaacttgtca agaaggtgta acctatggat
    18301 agatgaaact attgtcttat tttgaaactc ttcaaatact tgttcatgta ttttcatctc
    18361 attcatacta tcaacactac ttgtaggttc atccattaaa ataatttcac tttttttaga
    18421 tgcaagtagt cctcttgcaa gagccaatct ttgcttttct cctccactca atgaaacccc
    18481 tttctcctga acactggtgt caagtccttt aggaagctta gataaaacag ggtttaatct
    18541 agctattttt atcacttttt tcaaatcttc ttcagatgct ggaagatcca ttgttatatt
    18601 atatctaaaa gtattattga atatttcagg ttcctgaggt attaatgtga cttgttcttt
    18661 aactctatta atccctcctt caagaggaat accatcgcaa taaatttccc ctttctcaac
    18721 cttatacaat cctcttatta aagaaagaat tgtgctctta ccagaaccac tctctccaac
    18781 aagtgcaatc ttttcttttt tctttaattt aaaagaaaca ttatcaagat gatttctttt
    18841 tccatctgta tcatatgtaa aagaaagatt cttgaatttt atttccttcc agccccttgg
    18901 aagtcttcca gattcaggaa attctaattt attatattct tcatccaaag gattagcacc
    18961 tataatccta gcatttgcac gtactacatc tccatataac caagcaaact tatagaaagt
    19021 ctgtcctaca ttattaagat aaccatacac catatataat gtcccaatta aaatagttcc
    19081 tgtaagatta aattctgtat aagatttgta aataagcgaa attacaatca taatttgaat
    19141 tgcaacactt gcaaatcccc atttaatctc atttataatt gaagagcttt tgaaagtctt
    19201 ataacttgca atctgtctag attcaatttc tttagaaact gtttttctta atctcaaagt
    19261 tatcacagtt attacattac tcaaataatc aaatatagta gcagatagtt tattgttata
    19321 ttcatttaat tttttatatt ttctttcaag gtttttatct actcttgata taataaataa
    19381 gattattaaa gcatagacaa atgcaaataa tcctattctc caatcaataa aaaacaaaat
    19441 aattattgaa ccaaagaagc ttatcaatcc atagagaata tcatgagtca tatgagaaga
    19501 aaattcttca agagcatttg aagctctatt tattttatct attgtgtcac cagaatgatt
    19561 atctttgtgc catttaacag gcaatttcaa gaccatcctt attttatcat ttacataatt
    19621 cttctttaca tgaaatccag ttatagtttc caaaaatctt gctggaccat gaagcatcca
    19681 aaaaacaact gttatgaaca aaagaagagc gattttcttc attaaaacaa caagttcttc
    19741 gtgagttgta attgattcct gaatagaatt aaatattgta cctataacat atggagttaa
    19801 aagagaaaca attcctgcaa taagaaacaa aaatatgtaa aaaataaacc attttcttct
    19861 ttttcctaaa tacccccact ctgttttaat agcattaata atcggatgcg tcattttatc
    19921 cctctttctc ttcaaaaaga aaataggtta tttaaaagtt atttaaattc cactgcatta
    19981 tttttataag aaatttattt taatgctaac aaacttatca actcaaattt taaaaacaac
    20041 ccattcctat tattaaagta aaagatggtg aaaaaacaaa acaaaaaagc aaataaaaaa
    20101 gaagatattt tgcttaatat ctttcatgaa gagcaagaca aaaaaggtta tatctcaata
    20161 gatttcttaa aaaaaataag cacaaaatat aatattccaa tttcaagact ttatggggtt
    20221 gttaaattct atactatgtt aagaacagaa cctcaaggaa aatacataat agaattatgt
    20281 ggttctccaa cttgtgtttt acatgaaagc agagaaatag aaaacttcct taaaaaagaa
    20341 ctcaaaatag atataggaga tacaacaaaa gacaaaatgt tctctgttta caaaacatct
    20401 tgtataggat gttgcgatga acctcctgca atgcttctca atggtaaacc aataacaaat
    20461 ttgactattg aaaaagtaaa gaaattaatc aaggaattaa aatcaaagaa aaaataaaaa
    20521 tgcaaatcct aaaaaaaacc aatcttcaag cactgaaact tgcaaaaaaa ctaggtcctg
    20581 aaaaagttct tcagatttta gaaagaaaat atcttgttgg aagaggagga gcaggttttc
    20641 ctcttgcaaa gaaatggaaa ttagcattag atgcaaaaaa gaaaaatcct tctaaagaaa
    20701 ttttcattat atgcaatgca gatgaaggtg aaccaggaac atttaaagat aaattcatcc
    20761 tgaaaaacaa tccagagaca ctaattgaag gaatgctcat agcttcctat gtattgaata
    20821 caaaaaaagc tttcatttat ttaagaaatg aatatgaaca tctcttaaaa gaccttagaa
    20881 aaacaatttc ctctgttcta aaaaaatcaa aaatggaaaa caaggtaaaa attgaaatag
    20941 taagaggtgc aggagcatat atttgtggag aagaaactgc aataatgcaa tcaataatgg
    21001 gttatagagg acaatctcaa tataaacctc cttatccaac tgaacaaggt ttatgggaaa
    21061 gtccaactgt cctcaacaat gttgaaactc ttacttgtgt tccacaagct cttctataca
    21121 acgactggaa tccaaactta agactttttt caacatcagg agatattgaa aaaccaggaa
    21181 tttatgaatg ggaattaggt gttaaaatgt caacaattct tgaaacagtt aaaccaactc
    21241 aagaaataaa agcaatttcc tttggatgct ttggaggaat aatgccaata aacaaaaaca
    21301 cattagttac agctgaaaca atctgtgaaa aggactgtca acatggagca ttcactctaa
    21361 ttttcatagg aaaaaacaga aatattgtag atatatgttt aagtatagct aaattctata
    21421 cttatgaatc ttgtggtaaa tgcactccat gtagagaagg aacaatccgt atcctaaatc
    21481 tcctaaaaaa agtacgtgat aaaaaagcaa ctcaatctga tttagatctc ttaaaagagc
    21541 tctcatacca tatcaaagaa accagtctct gtggtttagg ccaatcttca acaaatcaca
    21601 tcctcacatc acttcaacac ttcaaggagg attataatta ttacttaaaa aaataaaatg
    21661 aaaataacat taaacaacaa acaaattaaa attgacccaa ataaaaccat ccttcaaatt
    21721 tgcaaagaga ataacataaa aattccaaca ctctgctatc acaaaaatct tttcccagaa
    21781 gcaagatgca gagtttgttt agttgaaatg aatggaaaat tagtaacttc ttgttcaact
    21841 aaacccgtgg aaaatgcaac tattattact aattctgaaa aagtaatcaa agcaagaaaa
    21901 atgaatcttg agcttatgaa tcaaaacatt aaattaaatg aactttcaga tgattttgaa
    21961 gcaagagaag tttatgatga aattggaatt gaaaataata gatttcaatc acttaaagat
    22021 tattatccag atttaggtgc tgcaatagtt cgtgataata acaaatgtgt aaattgtgga
    22081 aagtgtgttc aggtgtgtgg aaaaattcag gaaatacatg caatagattt tgcaagtcgt
    22141 gctcacaatg aacatgtaac accttatggt gaaaaaaagc tagaacaagt agcatgtatc
    22201 caatgtggcc aatgtttaat taattgccct gttggagcaa taattgaaag aactcacttg
    22261 tctgaagttc ttgaagcatt gaaaaatcct aaaaaacatg ttgtagcaca aactgctcct
    22321 tcaataagag ccgcattagg agaactcttt ggaatgcctg caggaactct tgtaacagga
    22381 aaaatggttg catccctaag aagatgcggt ttcgataaag tatttgacgt agatttagga
    22441 gcagatatga caataatgga agaatcagct gaacttctaa aacgtctcaa agaaaaaaac
    22501 aaaaagaaac tcccaatgat tacaacctgt tgcccagcat ggatattaat gatggaatct
    22561 ttctatccag aacttattcc taatatgtct acttgtaaaa gcccgcatga aatgttagga
    22621 atgttagtaa aaagttatta tgcggaactt gcaaatatca atcctaaaga tattgtagtt
    22681 gttagtataa tgccatgtac agcaaagaaa tttgaatcaa cacgttcaga attaaacaca
    22741 ggagtggatt atgttttaac aacacgagaa cttggaagat taataaaaat gaaaaacatt
    22801 gattttgtaa atcttcctga tgaagacttt gatccaacac taggattagc aagtggagca
    22861 ggagatattt ttggtgcagc tggaggagtt atggaagctg cattaagaac agcttacgaa
    22921 atttcaacaa atcctccaaa aaaattagaa aaagttgaat tcaaaaatct aagaacagat
    22981 gacagctcta taagagaagg aacaataaaa ctaggaaaca aaacagtgcg ttatgcaaca
    23041 acttcaggtg gagcaaatat ccgcaagcta ttgaaaaaca aagacaaata tgatttcatt
    23101 gaaatgatgg cttgtccagg aggttgcatt ggaggcggag gacaacctat ttactctgat
    23161 gataaagttc tcacactaag agcccaagca ctttacaaac aagataacat gcgtaaatat
    23221 cgtcgttctc atgaaaaccc aatagtaaaa gatatttaca aaaaatatct cggcaaacct
    23281 ctcagcagaa aagcagaaaa g
//
LOCUS       DAOPCH010000006        22673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_2426_length_22673_cov_4.971748, whole genome shotgun sequence.
ACCESSION   DAOPCH010000006 DAOPCH010000000
VERSION     DAOPCH010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 22673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22673
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2426_length_22673_cov_4.971748"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PLU55_00785"
     CDS             <1..284
                     /locus_tag="PLU55_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86628.1"
                     /translation="EKDYSTVVYLYEYFSKDLTQKAKQNVWSKILSDLKQEQGRNILL
                     IPIASDTNLSSLNALTAKFNITSYPVVIINEKHVISDLSSVQELERYLK"
     gene            356..1180
                     /locus_tag="PLU55_00790"
     CDS             356..1180
                     /locus_tag="PLU55_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86629.1"
                     /translation="MKECFRCGVSEERERLFDAISGKGVIKLCKNCAHDEGLPLVQPV
                     DLNRPEKTRTVYERLSDMAKLDPEKHRSILLEKAKQDSMRRYKRQDTTLKGVVDTNFQ
                     KNKPQPRKDLIPNFHWVIMRARRAKKLTQKQLAENLGEPESLIISAESGVILNNADSL
                     VRKLENYLGIKIRNIESPYTSDVSKDLAVGTNEDPALREARERFEREEGSFDKETTEN
                     LTISDLQEINKKKESEGKGGLFSFFRRKKKKSQEEAVPNPEEGISSEEADRILFGN"
     gene            1235..1498
                     /locus_tag="PLU55_00795"
     CDS             1235..1498
                     /locus_tag="PLU55_00795"
                     /inference="COORDINATES: protein motif:HMM:NF025040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpoL/Rpb11 RNA polymerase subunit family
                     protein"
                     /protein_id="HPJ86630.1"
                     /translation="MEMDILKDSKEEIEVEMESLTLVEILRVYLNKDSAVTFAAWKRE
                     HPTKKPILAVKTKGKSAKKALSDAVDAITKDLNKIEDDFKKLK"
     gene            1540..2274
                     /gene="radB"
                     /locus_tag="PLU55_00800"
     CDS             1540..2274
                     /gene="radB"
                     /locus_tag="PLU55_00800"
                     /inference="COORDINATES: protein motif:HMM:NF020014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadB"
                     /protein_id="HPJ86631.1"
                     /translation="MKTEKISTGSYDFNTWLYGGYEKDIITMIAGPPGCGKTNFVILA
                     SCSQAKKGNKVIFVDTEGGFSVERVRQIVGSENYESVLNNIFLLKPTSFEEQKKDFVK
                     LLGLVKKDDIGLIVVDGMAMLYRLEIGDAYNSGDDPRVKVVNREIAKQMRIFAEISRK
                     QNIPIIITNQVYLEFLPEGDFKKGVERNTNIVGGDLFKYWSKCIIELKNDNGKRKAVL
                     LKHRNLPQKEIGFIIKNEGIFKKRWI"
     gene            complement(2271..2837)
                     /locus_tag="PLU55_00805"
     CDS             complement(2271..2837)
                     /locus_tag="PLU55_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86632.1"
                     /translation="MEERGNSEGMSKEDISKKLERFQTTSEKIEFLQYIEPKINSTNP
                     NTQKAYYETLGDLFLKKENFQEAAGYYKKAGLDEKAEKIWEKLGDIAKIYHEDDKAIE
                     YYKKSNSSEKEEELLKKKETHSLEDKFLVMLAFCTFLFSFVFFSGRITGNTIAQFPLS
                     SHNLIGIGLFIMGMIVTFLYSERKNKNN"
     gene            2950..4161
                     /locus_tag="PLU55_00810"
     CDS             2950..4161
                     /locus_tag="PLU55_00810"
                     /inference="COORDINATES: protein motif:HMM:NF016765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86633.1"
                     /translation="MTEAIKFETVKKVADVSFKIKDLSKISKVDSISPPSVFIGSKLK
                     YPLVNVGILSPLNKDDNAWLYDDAKYWAENNLNIEEVMSLRNGLLNSRFQAKVSDIRL
                     NKKFVEVAKDVAISSKQVDVEIELKNRLIVGREKDKILTPHGMRAGLKNVAITGNVKV
                     EKQVDKVINDEIKASEAIEFLYKNEFNEYTLSKILSVGVIGLAKNKKLVPTRWSITAT
                     DDILGKKFLENIRQYPWIENYELFFGEFMGNQYIILMFPSSWSYELFELYLPHSSWNP
                     SQEIKASTDIEGYGGRKSYAFDTAGGYYASRFSILEYLNLIKRQASVIAIRVETPSYW
                     ASLGVWVVRESVKKALKREMKFADRSELLESVKKIGKIKYNFDFENILNRSKLLREKK
                     EQKSLKDFVKI"
     gene            complement(4163..6562)
                     /gene="topA"
                     /locus_tag="PLU55_00815"
     CDS             complement(4163..6562)
                     /gene="topA"
                     /locus_tag="PLU55_00815"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HPJ86634.1"
                     /translation="MAIKKKKKKTKEELAIEGNFRVDKRDLKYATEKKVKKALPKQNI
                     LKNITEKPVKTTPTEIITQGAIEKHYKKPSKKQIEKVEKFIVLKKKVKKSKKAEEYSH
                     PKIKLRENGYELIITEKPQAALKIASALGSPTQKNFNKVPHYEVKRGNKKILVACAVG
                     HLFTLKQKNGGYQIPVFDISWIPNFLAKRGDFTKRYYDSILKLAKEASSITVATDYDI
                     EGEVIGLNIVRYICNQQDASRMKFSTLTKTELEKAYDNKSPNINWGQAIAGETRHYLD
                     WFYGINLSRALMNAIKTTGKFKIMSIGRVQGPALHLIVQKEKEIAAFKPKPYWQIFIT
                     ISDKKNKLELKHTKDIFNKSQLEKFKDLKGKEVDVELIKKQEKVSPPSPFNLTTLQTE
                     AYKFYGITPSRTLQIAQSLYLAGLISYPRTSSQKLPPSIGYKEILNKLAKIFKTEKLI
                     KRDKPIEGSKTDPAHPSIYPTGNTQILSGDEEKIYKLIVKRFLSLFCEDALIDNKTVK
                     ATLNDLVFSKRGQSIARKSWMEIYPTKLKEEEIPDMQGKAQIIDSRTEQKETQPPKRY
                     SPASIISELEKRNLGTKATRSSILETLYDRGYVQGQSIQATPLGISLINTLKKYSPII
                     IDEKLTRDFEIGMENIQRMHIKTKQEKKEEEIINKAKTTITEISKDFEKNKNKIGQEL
                     IDANIEYREQQKRENALIRCPKCGKGDLSINYSKKTRRFFVSCNAYPDCRNTYSLPPN
                     GNITKSDKICEECNWPMLTRLSKGKKPWTFCFNPQCPTNKEWAEKSAAKKEEAEAEKQ
                     E"
     gene            complement(6618..7241)
                     /locus_tag="PLU55_00820"
     CDS             complement(6618..7241)
                     /locus_tag="PLU55_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPJ86635.1"
                     /translation="MKKRNKKTKILAVGDIHGDTKFVERLAKKAKDEEVDLVILAGDL
                     TFLEKSTKNLIGPFVKEKKEVVLIPGNHETMSTINTLTQIYEGTKHVHGYSVRKGDLG
                     IFGSGYEPSTGPFFIEDEEIFKVLKKGNEKIKDSKKKIMVTHAHPKGSVAEFSGFPGS
                     NAVKKAIKEFKPDILISAHIHEAGGLQEKIGKTKIIHVGRKPKIFEI"
     gene            7416..7694
                     /locus_tag="PLU55_00825"
     CDS             7416..7694
                     /locus_tag="PLU55_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HPJ86636.1"
                     /translation="MEQGKKDNFDIFFRRKPALMLVSLKKNTRMRYGSILAKEVDCTY
                     SHAVKILQTLEELKLVEFEKKGRIKVIKLTKKGIEVAEAIENIQKLIR"
     gene            7800..8150
                     /locus_tag="PLU55_00830"
     CDS             7800..8150
                     /locus_tag="PLU55_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86637.1"
                     /translation="MVSSSYKGIKFPPLTNKEIEEKYKEAEEEMQEVLEWKKEEEARL
                     KDKKSKPQAISAAKRALIKVERRINTVNGNLIYWKLRKEGKSHFYANLERNEYWDKLK
                     NGSSGNDDKESEDD"
     gene            complement(8159..8233)
                     /locus_tag="PLU55_00835"
     tRNA            complement(8159..8233)
                     /locus_tag="PLU55_00835"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8196..8198),aa:Lys,seq:ctt)
     gene            complement(8317..8622)
                     /locus_tag="PLU55_00840"
     CDS             complement(8317..8622)
                     /locus_tag="PLU55_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014011749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HPJ86638.1"
                     /translation="MEFCPKCGAVLIQKRKNYGCARCDYSTKEKLKIKTSEKIEEKSK
                     VAVVKKEMNTLPLVVEKCKKCGNDKAYFWTSQTRAGDEAETKFFKCTKCNHTWREYR"
     gene            complement(8695..9333)
                     /locus_tag="PLU55_00845"
     CDS             complement(8695..9333)
                     /locus_tag="PLU55_00845"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPJ86639.1"
                     /translation="MTRGGNKSKFRKFLKRFWEIVWKDDSFKGWLISLLFIFIVIKFI
                     FFPLLNLVTGTTLPLAIVESCSMYHSGNVFGNFNSWWDRHEIKYSSLNIDKTQFQEFP
                     LKKGFSKGDIVFIVGTKPEKLEVGDIIIFESDQKNPVIHRIIEIRQENGKYIFSTIGD
                     NNYGQISGEKSISEDQLVGKAAANILPSVGWIKLIFYEHLRTPSERGMCNEN"
     gene            9533..9697
                     /locus_tag="PLU55_00850"
     CDS             9533..9697
                     /locus_tag="PLU55_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86640.1"
                     /translation="MEYVINNYSIGKSSFNYDIANYREMYSQNSFAQQMYKNIVRYVP
                     TTINIGDYIK"
     gene            9759..10202
                     /locus_tag="PLU55_00855"
     CDS             9759..10202
                     /locus_tag="PLU55_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86641.1"
                     /translation="MKKTKFVKNGFVLRGVVCPNESCNEKIIHPIDEQEYNKFINLRN
                     KEFKVKMRLVGNSYAVSIPKEIVSFMNEQEKIMNDMVKLCFEDFGKLSLSFGDYVDGD
                     SRVIKSRECKVIKNNKPVLHTKQFYDSANPENNRKMIIKEDGGDE"
     gene            10205..12535
                     /locus_tag="PLU55_00860"
     CDS             10205..12535
                     /locus_tag="PLU55_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HPJ86642.1"
                     /translation="MTTKKIQLKVVEALQDDAYKGIARIDADLMKELDLKRGDAIIIK
                     GNRETVAIVDRSYPADVGEGIIRIDGIQRKNAKTSLGEVVSISKAEIKEAKKIMIAPA
                     QKGIMVQGDPQGLRTGLLGRTVVKGDIVVLGGVQRRKDLMSEEFGDLEDVFGNLNELL
                     GGMGFGNLGGRISQIKFLVVNVNPNQPVRITENTEVTLNSKAVEINEENIPEINYEDI
                     GGLSEEIRKIREMVEIPMKHPEIFERLGIEPPKGVLLHGPPGTGKTLLAKAVANESEA
                     NFILLNGPEIMSKFYGESEKKIRDIFEEAEKTAPTIIFIDEIDAIAPKREDVQGEVER
                     RVVSQILTMMDGLKGRGKVIVIGATNRVNAIDPALRRPGRFDREIEVGVPSKDGRLQI
                     LKIHTRGMPLTKTVKLEELASLTHGFVGADLESLAKEAAMNVLRKYLPQMKLDGEDQI
                     PKEILEKLVIDQEDFREALRVVRPSAMREVLVETPNVNWEDVGGLENIKQELKEAVEW
                     PMKYPESFARMGIRPSRGILLYGPPGTGKTLLAKAVAKESEANFIQVKGPSLLSMWVG
                     KSEEGMRKIFERARQVSPCVIFFDEIDSLAGKRGVEMGTKVTERVLNQMLAEMDGLED
                     LKDILVMGATNRPDMLDPALLRPGRFDKILLVNAPDSKGRLNILKIHTKKMPLGDGKK
                     IFGEKEKENFLKELSEKTEGYTGADLESLAREAALLSLRESIESKFVTEKHFEGALKK
                     VKPSITKSVIDAYRKIEENFLKSAKSAVYEGNSYLG"
     gene            12600..13397
                     /locus_tag="PLU55_00865"
     CDS             12600..13397
                     /locus_tag="PLU55_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86643.1"
                     /translation="MEKLGVLVGYGFDFELGGQNKICAALKNNGYFVKSIGEPWPRGK
                     YVLFGKKYISSGELDDSGNVFGEGGNVQLGKDFVLVSDNAFHFNEIKKDVDLKRLISD
                     RGYYESSKKIISNKGKEQYNVPVHVAPTGYFFGNSSAQGHIDLFTLLLPNSKILIFDK
                     YFGKDANLNYDYNKICEKECLKFLEYDGSKEEVWFPLNSLVLPNSQKEKVVIDSKAKS
                     LNRILEKEGIERILVDMPQRNYPAGKINCQTNVFLLKDKNKLDKLLV"
     gene            complement(13394..14281)
                     /gene="map"
                     /locus_tag="PLU55_00870"
     CDS             complement(13394..14281)
                     /gene="map"
                     /locus_tag="PLU55_00870"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HPJ86644.1"
                     /translation="MEKKEIILAGKIAQDVKEWIKPQIKKGVPLLEIAEKIEKKIIEL
                     KGKPAFPTNLSINEIAAHYTPFHNDETLASGLLKVDLGVHVNGFVSDTSFSLDLENSE
                     ENKKLIEASKKALENAKKTIEKGIQINKIGKVIQNTIESYGFSPIINLSGHQIEQYNL
                     HAGLTIPNIDDKRTTEITAGLYAIEPFATNGYGKIKDGKPSGIYSLINHKNIRNNDAR
                     KILEYIIEEYNQLPFCSRWIVNVFGTRALISLSQLEQNGNLHQYAQLVEVGKGIVSQA
                     EDTILIEENGNKIITTSKD"
     gene            complement(14263..14496)
                     /locus_tag="PLU55_00875"
     CDS             complement(14263..14496)
                     /locus_tag="PLU55_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86645.1"
                     /translation="MHKTLKTIWVSTGILIFLWTAFWYFKYLEEPGITGNVLNCTFGL
                     YLLFLYVTVAIVYWINKWLYKSKDPNMKWKKRK"
     gene            complement(14535..16997)
                     /locus_tag="PLU55_00880"
     CDS             complement(14535..16997)
                     /locus_tag="PLU55_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase"
                     /protein_id="HPJ86646.1"
                     /translation="MKTNFIPLDYQPFDYEGKNYIQVFGRDENGKKVCVIDSCPVYLW
                     AILKDNIGKKELGDLIEEVDKISLDKKSRKTRIEKIEIHDKKFLGKTVEALKIFATNY
                     KDLHDLADNLDFQGIEKRRGYDLGFITHYIIERKLNPLEWYEIEGEMLNNSDKFGGID
                     SALEVDFCIELKSLKKIPEDKHKTKFFPKILAYDIEAKELELGKGEILMISLYGENFK
                     KVLTFKEPSKKTFVEKFKDEKEMLEGFVNEVKKYSPDILVGYFSDGFDLPYIKARTEK
                     LKIKLNLGLDNSQPIFSRGIFLTGKIKGIVHIDLLRFIKNAYSQYLQSETLSLNEVSN
                     ELLGERKIELSPLEESRKNKVDWDKFYEYNLKDSELTYKILEKIWADLEEITKITKEP
                     IFSVSRSTMAGNFEDYVLHNLERFNEIPEKKPYRPEIIERRNREKYQGAFVFQPTPGL
                     YENIAFFDFSSMYGSVIVTYNLSKSTQTEDKKNSYVGEFQNEKVYFSKEQGFVPELLK
                     EIIMKRRKYKEDYNKNPNPILKVRSNAFKLIANAAYGYQGFFGARYYSVEAASSTAYF
                     ARESIQKAIKIFEDEGFKPVYSDTDSIAVILGNKTKKQALDLLKKINKELPGIMELDL
                     EGFFKRGLWVTKRTGDFGAKKKYALIDEQDKIKIRGFETVRRDWCDLAREVQNKVIKL
                     VLEDGDEKQAIEYLKEIIKKIKSRKILKKEIIIRTQLKKSISEYKAISPHVIAAKKMK
                     ELSIAPNEGDLIEYYIAETAGKKSKLVREKVKLPQEEGEYNIEYYLKRQILPAVENIF
                     QVFNIDVKEIIEEKKQKKLSDF"
     gene            17140..17529
                     /locus_tag="PLU55_00885"
     CDS             17140..17529
                     /locus_tag="PLU55_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86647.1"
                     /translation="MVNKYTVFNYAPETYEKDKYLVDAIHRRVPYLSRGRPQDALELA
                     KILKEYGYKGLRVASNDSPNAFVSLDKLVSINPWRIIRVANDMYVQANKRMSFFGDPS
                     KNPESPFYKKGPDLEGRIEDDGQGRLF"
     gene            17586..18626
                     /gene="fen"
                     /locus_tag="PLU55_00890"
     CDS             17586..18626
                     /gene="fen"
                     /locus_tag="PLU55_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HPJ86648.1"
                     /translation="MGLNIREIIPRRELEESELKDKIVCVDAFNALYQFLSSIRQPDG
                     TPLMDNKKRITSHLSGIFYRNINLMEEGIKLVYVFDGRAPDLKYKTHKERETSRDIAK
                     ERYEIAKGELDIESMKRYSSQLTRLNREMIEESKELLEAMGIPVIQASSEGEAQAAYM
                     ARTIPRVYASVSQDYDSLLFGAPRLIRNLTVSRKRKTYSGYVEIKQEIIELDKVLNIL
                     EINLEQLICLGILVGTDYNPKGIPGIGQKKALDIVKKYKFPYEIFKSVEEQIMSLSEE
                     DRFDWQEIFSLFYKPKVDNVDFEFKGFDENKIREILVERHDFSEERVQKQINRLKDLK
                     NKNQQKSLGKWF"
     gene            complement(18629..19093)
                     /locus_tag="PLU55_00895"
     CDS             complement(18629..19093)
                     /locus_tag="PLU55_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86649.1"
                     /translation="MGVDIFYNLDAELIIFGLIFVLFFAIIRLALSKTIKDKGPLGVI
                     SLSIALLSVYGLSKTNLDFPGFFYKIGINNNLLYSVIPIIILVGLVYMILAVTARVIF
                     SFFGVLFIVGSFFLHNLVWWFRIIGIIFLIIGIVLIKREFKRRVDKRIYLRR"
     gene            19177..19965
                     /locus_tag="PLU55_00900"
     CDS             19177..19965
                     /locus_tag="PLU55_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86650.1"
                     /translation="MVNHRSFNILGNIAVVNFPYGTKLSDKKKFALEILNKNKFIKTV
                     VEKSGNFAGRLRKMETKHLAGEKNKEVLYKENNCSFRFNLDTTYFSPRLSNERKEIAS
                     CIKNNSSVLVMFAGVAPFSIVIAKNSKPKEVYSNEINRAANKYGELNAEINGVKNKIK
                     FVNGDIKKVAVKLKEEGKKFDFIVMPRPNLKESFLEQAFMLSKKGTIIFYYDFCKVDE
                     VDKILEKINKKALESGNRIKILNKKPAGEIAPYKIRIRVDFKVL"
     gene            complement(19952..20560)
                     /locus_tag="PLU55_00905"
     CDS             complement(19952..20560)
                     /locus_tag="PLU55_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86651.1"
                     /translation="MDKAINIAKEIISEVNKKVKRVYWTNEEIDKYFGRRSVEEILSS
                     GETCFMNPCLDLTLVSSHLISKKKIPHKLVIEEHLPSNGFDFNRLHFVIEFQNNDKQS
                     KEYFLNYKRANEVYLLEGKYNGRQDLPLAQILKIQGLKLNPKKPLYVNLGYKTLEEFS
                     KENFKGYSLEKNISRLKKDNSIENYQKYKQKFGEDFLIITRP"
     gene            complement(20595..21905)
                     /locus_tag="PLU55_00910"
     CDS             complement(20595..21905)
                     /locus_tag="PLU55_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HPJ86652.1"
                     /translation="MSYEIITGRNSSDKKKFEGKGLAYIGKGFVKMGQYTSLSNKIFM
                     DVIRSHVVLVAGKRGSGKSYTLGVIAEELSNLPKDVSQNIASLMFDTMGIYWTMKYAN
                     EKDKELLHEWDLKPKNLNVKIFIPFGFYDDYVRRGIPADEKFALDVTEMNAEDWILTF
                     GLDIIHPVAVLIQRIINKLRTKKIFDIDDIVNEIEKDQKTDREIKNAATGLFEAANTW
                     GIFSKKGISPTKITHLVNAGTTTVLDLSVYNSVGSFNVRALVISLISRKLFNQRMAAR
                     KKEEIKSISRGLDMGYYVEKKENPLIWLFIDEAHEFLPLEGKTIASDALTQLLREGRQ
                     PGISLVLATQQPGQIHKDVMTQSDIVISHRVTSAPDLEALNKIMQSYLLESIKQYMDE
                     LPSLNGSAIILDDNSERIYPMRIRPRFTWHGGEAPTALKAEKKF"
     gene            22002..>22673
                     /locus_tag="PLU55_00915"
     CDS             22002..>22673
                     /locus_tag="PLU55_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86653.1"
                     /translation="MENQPKEHIVHVNRERRHGIRDKPWVVSTIALIILVVLLLSLNV
                     FNITGKVVSANKAGEKLADFAESQGLTVEVSSVEKEGNFYLVTLLIEGQELPMYVTKD
                     GKYFTNYLYPLEEEEEKSNNNQNTNTQTQEIPKSEKPTVDLFVMSHCPYGTQAEKGII
                     PAIEALGDTVNFNLRFVYYIMHPSAGEVEEQTRQYCIQKEQEAKLLPYLKCFLGKTGT
                     AADSEA"
BASE COUNT         7518 a         2920 c         3695 g         8540 t
ORIGIN      
        1 gcgaaaaaga ttattctact gtggtttatc tctatgaata tttttctaaa gatttaactc
       61 agaaagcaaa gcaaaatgta tggtcaaaaa tattgtctga tttaaaacaa gaacaaggaa
      121 ggaatatact tttaatacct atagcttcag atactaacct ttcttctttg aatgctttaa
      181 ctgctaaatt taatattaca agttaccctg ttgttatcat caatgaaaag catgtaattt
      241 ctgaccttag ctctgttcaa gagcttgaaa gatatttgaa gtagtttttt gtttggtttt
      301 tttattaatt ttaataatac aaatattaaa aaagggataa ttactaagat tttttatgaa
      361 agaatgtttt cgctgtggtg tttctgagga aagagaaaga ttgttcgacg ctatttcagg
      421 aaaaggggtc ataaaattat gtaaaaattg cgctcatgat gagggtttgc ctttagtcca
      481 accagttgat ttaaataggc ctgaaaagac aagaacagtt tatgaaagac tttcagatat
      541 ggctaaatta gatcctgaaa aacataggag tattcttctt gaaaaggcta agcaggattc
      601 aatgagaagg tataaaagac aggatactac cttaaaagga gttgtggata ctaattttca
      661 gaaaaataaa cctcaaccta gaaaagattt gattccaaat tttcattggg ttattatgag
      721 agccagaaga gctaagaaac ttactcaaaa acaattagca gaaaatcttg gagaaccaga
      781 atctttgatt attagtgctg aatcaggagt tattttgaat aatgcagata gtcttgtaag
      841 gaaacttgaa aattatcttg gaataaagat taggaatatt gaaagccctt atacttctga
      901 tgtttctaag gatttagctg ttggaacaaa tgaggaccct gctttaagag aagccagaga
      961 gagatttgaa agagaagagg gtagctttga taaggaaaca acagagaatt taactatttc
     1021 tgatttacag gaaataaaca agaaaaaaga atcagaaggt aaaggaggat tattttcatt
     1081 ttttagaaga aagaaaaaga aatcacaaga agaagctgtt ccaaatcctg aagaaggaat
     1141 ctcaagtgaa gaagcagata gaatactttt tggtaactaa tcactaaaaa atactataag
     1201 taaagttttt aaactaattt tatgataaac tatcatggaa atggatattc ttaaggactc
     1261 aaaggaagaa attgaagttg aaatggaaag tcttactttg gtggaaatat tgagggttta
     1321 tttaaataag gattctgcag ttacatttgc tgcttggaaa agagaacacc ctacaaaaaa
     1381 acctatctta gctgttaaaa ctaagggaaa gagtgctaag aaagctttaa gtgatgctgt
     1441 tgatgcaatt acaaaagatt tgaataaaat tgaagacgat tttaaaaaat taaagtaaat
     1501 acaataatac ttattaattc tatagttgat tagattttta tgaaaacaga aaaaatatct
     1561 acaggaagtt atgattttaa tacttggttg tatggaggat atgaaaaaga cattataact
     1621 atgattgcag gccctcctgg ttgtggaaaa actaattttg taatattggc ttcgtgtagt
     1681 caggctaaaa aaggaaataa agttattttt gtggatacag aaggaggatt tagtgttgaa
     1741 agagttagac agattgtagg ttctgagaat tatgaatctg ttctgaataa tatttttctt
     1801 ttgaaaccaa ctagttttga agaacagaaa aaagattttg ttaaattact tggacttgtt
     1861 aaaaaggatg atattggttt aattgttgtt gatggaatgg ctatgcttta tagattggaa
     1921 attggagatg catataattc tggagatgat ccaagagtta aagtagtcaa cagagagatt
     1981 gccaaacaaa tgaggatatt tgcagagatt tcaaggaaac aaaacatccc cataatcatc
     2041 acaaatcaag tttatctgga atttcttcca gaaggagatt ttaaaaaagg agttgaaagg
     2101 aacacaaata ttgttggagg agatttgttc aagtattgga gtaaatgtat aattgaatta
     2161 aagaatgata atggaaagag aaaggcagtt cttttaaaac ataggaattt gcctcaaaaa
     2221 gaaataggat ttataattaa aaatgaaggt atattcaaga aaaggtggat ttagttgttt
     2281 ttattttttc tttcagagta aaggaaggta acaatcattc ccattataaa taatccaatt
     2341 cctattaagt tgtgagagct taaaggaaat tgtgctattg tatttcctgt tattcttcca
     2401 gagaagaata caaaagaaaa taaaaatgtg caaaaagcaa gcataacaag gaatttatct
     2461 tcaagagaat gagtttcttt tttctttaat agttcttctt ctttttctga agaatttgat
     2521 tttttataat attctattgc tttatcatct tcatgataaa tttttgctat atctcctagt
     2581 ttttcccaaa ttttttctgc cttttcatct aatccagctt tcttataata accagctgct
     2641 tcttgaaaat tctctttttt aaggaataaa tctcctagag tttcataata tgctttttga
     2701 gtgtttggat tagttgaatt tatttttggt tctatatatt gcaaaaattc tattttttca
     2761 gaagttgttt gaaatctttc taattttttg gagatatctt ccttactcat cccctccgaa
     2821 tttccccttt cttccattat ttaactaacc ttttttcatt taaataggtt atgaattttt
     2881 attttttgtt ttgttgtaat ttattaagta gttacaaact catttaaaga ttctttttgt
     2941 ttatgactta tgacagaggc gataaaattt gaaacagtta agaaagttgc agatgttagt
     3001 tttaaaatta aagatttaag taagatttct aaggtagatt ctatctctcc tccaagtgtg
     3061 tttattggaa gcaaattaaa atatcccttg gttaatgttg gaatattaag tccattaaac
     3121 aaagacgata atgcctggct ttatgatgat gcaaaatatt gggcagaaaa taatcttaat
     3181 atagaagagg ttatgagttt gagaaatggg cttcttaact caagatttca ggctaaagtt
     3241 agtgatatta gattaaataa gaaatttgtt gaggttgcta aagatgttgc tatatcttca
     3301 aaacaagttg atgttgaaat tgaattaaaa aacagactta ttgttggaag agagaaagat
     3361 aaaatcttaa ctcctcatgg aatgagggct ggcttaaaga atgtagcgat aacaggtaat
     3421 gttaaagttg aaaagcaagt agataaagtg ataaatgacg agattaaggc atcagaagca
     3481 attgaatttt tatataaaaa tgaatttaat gaatatactt taagtaaaat attgagtgtt
     3541 ggtgttatag gtttagcgaa aaataaaaag ttagttccca caaggtggag catcaccgca
     3601 acagatgata ttttaggtaa gaaatttttg gagaatatac gtcaatatcc ttggatagag
     3661 aattatgaat tattttttgg agagtttatg gggaatcaat acattatcct tatgtttcca
     3721 agttcttgga gttatgagtt atttgaatta tatttacctc atagttcatg gaatccaagt
     3781 caagaaatta aagcatctac tgatattgag ggttatggag gaagaaagag ttatgcattt
     3841 gatactgcag gaggatatta cgcttctcgt ttttcaattc ttgaatattt aaatttaatt
     3901 aaaaggcaag caagtgtaat tgcaataaga gttgaaactc ctagttattg ggcgtctcta
     3961 ggagtgtggg tagtaagaga atctgttaag aaggctttga aaagagagat gaagtttgca
     4021 gatagaagtg aacttttaga gagtgttaaa aaaataggta aaataaaata taattttgat
     4081 tttgaaaata ttttgaatag gagcaaactt ttaagagaaa aaaaggaaca aaaaagttta
     4141 aaggattttg tcaagatata atttattctt gtttttctgc ttcagcttct tctttttttg
     4201 ctgctgattt ttcagcccat tctttatttg ttggacattg aggattaaaa caaaatgtcc
     4261 acggtttttt acctttggat aatcttgtta gcataggcca attacattct tcacatattt
     4321 tatctgattt tgttatgttt ccatttggag gtaaactata agtgtttcta caatcaggat
     4381 aagcattaca acttacaaag aatcttcttg ttttcttgga atagtttata gataaatctc
     4441 cttttccaca ttttggacat cttatcaatg cattctctct tttttgctgt tctctgtatt
     4501 ctatgtttgc atcaattagt tcttgtccta ttttattttt atttttctca aaatcttttg
     4561 aaatctctgt tattgttgtt tttgctttat ttattatttc ttcttctttt ttttcttgtt
     4621 ttgtttttat gtgcattctt tgaatgtttt ccattcctat ttcaaaatct cttgttagtt
     4681 tttcatctat aattattgga gagtattttt ttaaagtgtt tatgagagat atgcctaatg
     4741 gggttgcttg tatgctttgt ccttggacat atcctctgtc atataatgtt tctagtattg
     4801 aagaacgagt ggcctttgtt cctagatttc ttttttctaa ttctgagatt attgatgctg
     4861 gagagtatct ttttggaggt tgtgtttctt tttgttctgt tcttgaatca attatctgtg
     4921 cttttccttg catgtctggt atttcttctt cttttaattt tgtcggataa atctccatcc
     4981 aagattttct tgctatggac tgtcctcttt ttgaaaaaac taaatcattt aatgttgctt
     5041 tgactgtttt attatctatt aatgcatctt cacaaaataa actcaagaat ctctttacta
     5101 ttaacttgta tatcttttct tcatctcctg aaagaatttg tgtgtttcct gttggataaa
     5161 ttgaaggatg tgctgggtct gttttacttc cttctattgg tttatctctt tttattaatt
     5221 tttcagtttt gaatattttt gcaagtttgt ttagaatttc tttatatcca attgatggag
     5281 ggagtttttg agaagaagtt cttggataag agattaatcc tgcaagatat aaagattggg
     5341 ctatttgtaa tgttcttgaa ggagttatcc cataaaactt atatgcttct gtttgaagtg
     5401 ttgttaaatt aaatggagag gggggagaaa ccttttcttg cttttttatt aattcaacat
     5461 ctacttcttt tcctttcaaa tctttgaatt tttctaattg tgatttgttg aatatgtctt
     5521 ttgtgtgttt taattcaagt ttattttttt tatcagaaat tgtaataaat atttgccaat
     5581 aaggttttgg tttgaatgca gctatttctt tttctttttg tacaatcaag tgaagtgcag
     5641 gaccttgaac tcttcctatt gacattatct taaacttgcc tgttgttttt atagcgttca
     5701 ttaatgctct tgaaagattt ataccataga accaatctaa gtaatgtcta gtttctcctg
     5761 caattgcttg cccccaattt atatttggag atttgttatc gtatgctttc tcaagttcag
     5821 ttttggtaag ggttgagaat ttcatacgag atgcatcttg ttggttgcaa atatatctta
     5881 cgatatttaa tcctattact tctccttcta tatcataatc tgtagccact gttattgatg
     5941 aagcttcttt tgctagtttt aatattgaat cataatatct ttttgtgaag tcccctcttt
     6001 ttgctaaaaa attaggaatc caagaaatat caaatactgg aatttggtat cctccgtttt
     6061 tttgtttaag cgtgaataaa tgtccaactg cacatgcaac taatattttt ttgtttcctc
     6121 ttttaacttc ataatgaggt actttattga aatttttttg agttggactt ccgagagcac
     6181 ttgcaatttt aagtgctgcc tgtggttttt ctgttatgat taattcatat ccattttctc
     6241 ttaattttat ttttggatga gaatattctt ctgctttttt ggattttttt actttctttt
     6301 ttaacacaat aaatttttca actttctcta tttgtttttt tgagggtttt ttgtaatgtt
     6361 tttcaattgc tccttgagtt attatctctg ttggagttgt tttaacaggt ttttcagtta
     6421 tattttttag aatattctgc tttggtaaag ctttttttac ttttttctca gttgcatatt
     6481 ttaaatctct tttatcaact ctgaagttcc cttctatagc caactcttct tttgtctttt
     6541 ttttcttttt ctttatcgcc atttttgttg ctttcttaga attaatgtgt tttttgtgtt
     6601 tttataaatt ttgtgtgtca gatttcaaat atttttggct ttcttcctac atgaattatt
     6661 tttgttttac ctattttttc ttgtaatcct cctgcttcat gtatatgtgc tgaaattaaa
     6721 atatctggtt tgaattcttt tattgctttt ttaactgcat ttgacccagg aaaacctgag
     6781 aattcagcta cacttccttt aggatgagca tgagttacca ttatcttttt tttagaatct
     6841 tttatttttt catttccttt ttttaatact ttaaatattt cttcatcttc tatgaaaaat
     6901 ggtcctgtag atggttcgta tccagaaccg aagattccaa ggtcaccttt tcttacagaa
     6961 tatccatgca cgtgttttgt tccttcgtag atttgagtta atgtatttat tgtggacata
     7021 gtttcatgat ttccaggtat taatacaact tctttttttt cttttacaaa aggtcctata
     7081 agattttttg ttgatttttc aaggaaagtt aagtctcctg caagaataac taaatcaact
     7141 tcttcatctt ttgctttttt tgctaatctc tcaacaaact ttgtatctcc atgaatatct
     7201 cctactgcaa gaatttttgt tttcttattt ctctttttca ttgattataa aatataagtg
     7261 agttaataag attttttatt tttagtttgt tgtaattatg aaatcattaa attgttaaat
     7321 tttcatcaaa attatgtagt gaatatatac attacaaaac atttaaaagg atgatattgt
     7381 ctggatatat acattaaata agacacctca ataagatgga acaaggaaag aaagacaatt
     7441 ttgatatctt tttcagaagg aagccagctt taatgcttgt ttctttgaag aagaatactc
     7501 gaatgaggta tggtagtatt ttggctaagg aagtggattg cacttatagc catgcagtga
     7561 aaattctgca gactttggaa gaacttaagt tagtggagtt tgaaaaaaag gggagaatta
     7621 aggtgataaa actaactaag aagggaattg aagttgcaga ggctattgaa aatattcaaa
     7681 agctaataag ataagattgc attgaggtga ttactaaata cccctgttgc gaatggaatt
     7741 caaatagttt tatttgaaat gatagcaatt tttattaact ctttttattt tttttattca
     7801 tggttagcag tagttataaa ggaataaagt tccctccttt aacaaataaa gaaatagaag
     7861 aaaaatataa agaagccgaa gaagagatgc aggaagtttt ggagtggaaa aaagaagagg
     7921 aagcaagact taaagataaa aaatcaaaac ctcaggcaat tagtgctgct aaaagagctt
     7981 taataaaagt tgaaagaaga ataaatactg ttaatggaaa tttaatttat tggaaattaa
     8041 gaaaagaagg aaagagtcat ttttatgcaa atcttgaaag aaatgaatat tgggataagc
     8101 tgaagaatgg aagttctggg aatgatgata aagaaagtga agatgattaa aaaagaagtg
     8161 accccgatgg gatttgaacc cacgacacct agattaagag tctagtgctc taaccaggct
     8221 gagctacgga gtcctggtat ataaattaat tagaaataaa tcgtttaaaa agatgttgtt
     8281 aaatttaata tttaagcatt ctctattttt taacatctat ctatattctc tccaagtgtg
     8341 attacactta gtgcatttga agaattttgt ttctgcttca tctcctgcac gtgtctggct
     8401 tgtccagaaa taagctttat catttccaca tttcttgcat ttttcaacaa ctagaggtaa
     8461 tgtattcatt tcttttttaa ctactgcaac tttgcttttt tcctctatct tttcagaagt
     8521 ttttattttt aatttttctt ttgtagaata atcacatctt gcacaaccat aattctttct
     8581 tttttggatt aaaactgctc cgcatttagg acaaaattcc attgtttatt tttactctcc
     8641 tttaacttct ttttttaaat ttattttctt tataaaactt cttgttgttt acttttagtt
     8701 ttcattacac attccccttt cagatggggt tcttaaatgt tcataaaata tcaatttaat
     8761 ccaacctact gaagggagta tgtttgcagc agcttttcct actaattggt cttcacttat
     8821 agatttttct ccacttattt gtccgtaatt gttatctcct attgttgaaa agatatactt
     8881 tccgttttct tgtcttattt caattattct atgtattact ggattttttt ggtcgctttc
     8941 aaaaattatt atgtctccta cttctagctt ttcaggtttt gtccctacta taaatactat
     9001 atctccttta ctgaatcctt ttttaagagg aaattcttga aattgagttt tgtctatatt
     9061 taatgaagag tattttatct cgtgcctatc ccaccaacta ttgaaatttc caaatacatt
     9121 tcctgagtga tacatgctac aagattcaac tattgcaaga ggaagagttg ttcctgttac
     9181 taaatttaat agagggaaga aaatgaattt tataactata aatataaaaa gaagagaaat
     9241 taaccatcct ttgaaactat cgtctttcca gactatttcc cagaaccttt ttaagaattt
     9301 tctaaattta cttttgttcc cacctctagt cattagagta aagatgaatt aaggttttta
     9361 aaatgttagt ttgtttttta tttgatttat atttttcaag aatagaaata tttaaatagt
     9421 atacatttct ttgtgtatac gtaatgtata tacaaaacct cttttcaacc cagccacact
     9481 cagggtaaaa ccttgagtta gtggctttta tatttatcaa ataaaaaaca aaatggaata
     9541 cgtgattaat aattatagta ttggaaagtc atcttttaat tatgatattg ctaattatag
     9601 agaaatgtat tctcaaaatt cttttgctca acaaatgtat aaaaatattg taagatatgt
     9661 tccaactaca attaatatag gagattatat taaataaaac taaaatgata aacgacatat
     9721 ttgataacac tattttatgc aggaaatgcg gtaaaaaaat gaaaaaaaca aaatttgtaa
     9781 aaaatggttt tgttttaaga ggagtggttt gtcctaatga atcttgcaat gaaaaaataa
     9841 ttcatcctat tgatgaacaa gagtacaaca aatttataaa tcttagaaac aaggaattca
     9901 aagtcaaaat gagactagtt ggaaatagtt atgctgtttc aatcccaaaa gaaatagttt
     9961 cgtttatgaa tgaacaagaa aaaattatga acgatatggt aaaattgtgt tttgaagatt
    10021 ttggaaaact gagtttaagt tttggggact atgttgatgg agattcaaga gttatcaaat
    10081 ccagagagtg taaagttata aagaataata aacctgtttt acacacaaaa caattttatg
    10141 attcagcaaa tcctgaaaat aatagaaaaa tgataattaa agaagatggg ggagatgaat
    10201 aaaaatgaca acaaagaaaa ttcagcttaa agttgtagaa gcattacaag atgatgcata
    10261 taaaggtatt gcaagaatag atgcagattt aatgaaagaa ttagacttga aaagaggaga
    10321 tgctataata attaaaggga atagagagac agttgcaata gttgataggt cttatcctgc
    10381 agatgttgga gaaggaatta taagaataga tggtattcaa agaaaaaatg ctaagacaag
    10441 tcttggagaa gttgtttcta tttctaaggc agagataaaa gaagcaaaaa aaataatgat
    10501 tgctcctgct caaaaaggaa taatggttca gggagatcct caggggttga ggactggttt
    10561 gctgggaaga actgttgtta aaggagatat tgttgtattg ggtggagttc agagaagaaa
    10621 agatttgatg tctgaagaat ttggagattt agaagatgtt tttggaaact taaatgaatt
    10681 attaggagga atggggtttg gaaatcttgg aggaagaatt tctcagatta aatttttagt
    10741 tgtgaatgta aatccaaatc aacctgtaag aataactgaa aacactgaag ttacattgaa
    10801 ttcaaaagca gttgagataa atgaagaaaa tattccagaa ataaattatg aagatattgg
    10861 tggtttgagt gaagaaataa gaaaaataag agagatggtt gagattccta tgaaacatcc
    10921 tgagattttt gaaagattgg gaattgaacc tccaaaagga gttcttttac atggacctcc
    10981 tggaacaggt aaaaccttac ttgctaaagc tgttgcaaat gaatcagaag caaattttat
    11041 tttacttaat ggtccagaaa taatgtcaaa gttttatgga gaatcagaaa agaaaataag
    11101 agatattttt gaagaagctg aaaaaacagc ccctacaatt atttttatag acgaaataga
    11161 tgcaattgct cctaagagag aggatgttca gggagaagtt gaaaggagag ttgtttcaca
    11221 aatattaact atgatggatg gtcttaaagg aagaggtaaa gttattgtta ttggagcaac
    11281 aaatagagtt aatgcaattg accctgcatt aagaagacca ggaagatttg acagagaaat
    11341 tgaagttgga gttccaagta aagatggaag attacagata ttgaaaatcc atacaagagg
    11401 aatgccttta actaaaacag ttaaattaga ggaattagct tcacttactc atggttttgt
    11461 tggagcagat ttagagtcct tagctaaaga agctgcaatg aatgttctta gaaaatattt
    11521 gcctcaaatg aaattagatg gcgaagacca aattccaaaa gaaatacttg aaaaattagt
    11581 tattgaccag gaagatttca gagaagcatt gagagttgta agaccaagtg caatgagaga
    11641 agttttagtt gaaactccta atgttaactg ggaagatgtt ggaggattag aaaatattaa
    11701 acaagaatta aaagaagctg ttgagtggcc aatgaaatat cctgaaagct ttgcaagaat
    11761 gggtataaga ccttcaagag gtattttatt atatggacct cctggaacag gaaaaacctt
    11821 gcttgctaaa gctgttgcaa aagaatcaga agcaaatttt atacaggtta agggtccttc
    11881 tttattgagt atgtgggtag gaaaatctga agaaggaatg agaaaaatat ttgagagagc
    11941 aagacaagta tctccttgtg ttatattctt tgatgaaata gattcattag caggtaagag
    12001 aggggttgaa atgggaacaa aagttacaga aagagttttg aatcaaatgc ttgcagaaat
    12061 ggatggatta gaagatttga aagatatttt ggttatgggt gcaacaaata gacctgatat
    12121 gttggaccct gcgttgttaa gaccaggaag atttgataag attcttttag ttaatgctcc
    12181 tgattcaaaa ggaagattaa acattcttaa gatacacact aagaaaatgc ctttaggaga
    12241 tggtaaaaaa atatttggtg aaaaagaaaa agaaaatttc cttaaggaac tttctgaaaa
    12301 aacagagggt tatactggtg cagacctaga atctttagca agagaagcag ctttactctc
    12361 attgagagaa tcaattgaat ctaagtttgt gactgagaaa cattttgaag gtgctcttaa
    12421 gaaagttaaa ccaagtataa ctaaatcagt tattgatgct tacagaaaaa tagaggaaaa
    12481 tttcttgaaa tctgcaaaga gtgctgttta tgaaggaaat agttatcttg gataaattgt
    12541 tatttctgta tagttacaac aatagtaagt tttaaatatt ctttttgttg cattccttta
    12601 tggaaaaatt aggagtatta gtaggatatg ggtttgattt tgaacttgga gggcaaaata
    12661 aaatttgtgc tgctcttaaa aataatggat attttgttaa atcaataggc gagccttggc
    12721 ctagaggtaa atatgttctt tttggaaaaa aatacatatc ttcaggagaa ttagatgatt
    12781 ctgggaatgt ttttggagaa gggggaaatg ttcaattagg taaagatttt gttctagttt
    12841 cagataatgc ttttcatttt aatgaaataa aaaaagatgt tgatttgaaa agattaatta
    12901 gtgatagagg ttattatgaa tcttcaaaaa agattatttc aaataaagga aaagaacaat
    12961 ataatgtgcc tgttcatgtt gccccaacag gttatttttt tggaaattct tctgctcaag
    13021 gacatattga tttattcact cttttacttc caaatagcaa aatattaatt ttcgataaat
    13081 attttggtaa ggatgcaaat ttgaattatg attataataa aatttgtgag aaagaatgtc
    13141 ttaaattttt agagtatgat ggaagtaagg aagaagtttg gtttccttta aactctttgg
    13201 ttttacctaa ctcacaaaaa gaaaaagtag ttattgatag taaggccaaa tcattaaata
    13261 ggattttaga aaaagaagga attgaaagaa ttttggttga tatgccacag agaaattatc
    13321 ctgcaggtaa aattaattgt cagacaaatg tttttctctt aaaagacaaa aataaattag
    13381 acaaactttt agtttaatct ttacttgttg ttattatttt atttccgttt tcttcaataa
    13441 gtatagtatc ttcagcttga gagactatac cttttcctac ttcaactaat tgagcatatt
    13501 ggtgtaaatt tccattttgt tctaattgag ataaagaaat taaagccctt gttccaaaga
    13561 catttacaat ccatcttgag caaaaaggga gttgattgta ttcttctatg atatattcta
    13621 gtatctttct tgcatcatta tttcttatat tcttatgatt aatcaaagag taaattccag
    13681 aaggttttcc atcttttatt tttccataac cgtttgttgc aaaaggttct attgcatata
    13741 atccagcagt aatttcagtt gttcttttat catctatatt tggaattgtt aatcctgcat
    13801 gaaggttgta ttgttctatt tggtgtcctg aaagatttat tattggagag aatccataag
    13861 attcgattgt attttgtatg acttttccta ttttatttat ttgaattcct ttttcaattg
    13921 ttttttttgc attttcaagg gctttttttg atgcttctat taacttttta ttttcttctg
    13981 aattttctaa gtccagagag aaagaagtgt cagatacaaa tccatttaca tgaactccta
    14041 aatctacttt aagcaaaccg cttgcaagtg tttcatcatt gtgaaaagga gtataatgtg
    14101 cagctatttc atttattgag aggtttgtag gaaaagcagg ttttcctttt aattcaatta
    14161 tttttttttc tattttttct gctatttcaa gtaatggaac cccttttttt atttggggct
    14221 taatccattc ttttacatct tgtgcaattt taccagcaag aattatttcc tttttttcca
    14281 tttcatattt gggtctttac ttttatacaa ccacttattt atccaataaa caatagcaac
    14341 tgttacatat aaaaagagta aataaagccc aaatgtacaa ttaagaacat ttcctgtaat
    14401 tcctggttct tctaaatatt taaaatacca gaatgctgtc cagagaaaga ttagtatccc
    14461 agtggatacc cagattgttt ttaatgtctt atgcatattt ttattagtag aagttagttt
    14521 aattaatttt tcttttagaa atcacttagt tttttctgtt ttttttcttc tattatttct
    14581 tttacatcta tgttgaatac ttgaaaaata ttttctactg caggaagtat ttgtctttta
    14641 agatagtatt ctatgttgta ttctccttct tcctggggga gcttaacttt ttctcttact
    14701 aatttagatt tctttcctgc ggtttctgct atatagtatt caattaaatc tccttcattt
    14761 ggtgctattg aaagttcttt catttttttt gcagcaatta catgaggaga gattgctttg
    14821 tattctgaaa ttgatttttt taactgagtc cttattatta tttctttttt gagtattttt
    14881 ctagatttta ttttttttat tatctctttt aaatattcta ttgcttgttt ttcatctcca
    14941 tcttctaaaa ctagttttat gactttgttt tgcacttctc ttgctaaatc acaccaatct
    15001 ctccttactg tttcaaatcc tcttattttt attttatcct gttcatctat taatgcgtat
    15061 tttttctttg ccccaaaatc tcctgttctt tttgtaaccc ataatcccct cttgaaaaat
    15121 ccttctaaat ctaattccat tattcctgga agttctttat ttattttctt aagtaaatca
    15181 agagcttgtt tttttgtttt atttcctaag attactgcta tactatctgt atcactataa
    15241 actggtttga acccttcatc ttcaaagatt tttattgctt tttgtatgct ctctcttgca
    15301 aaataagctg ttgatgaagc agcttcaaca gaataatatc ttgctccgaa aaatccctga
    15361 tatccataag ctgcatttgc aatcagtttg aaagcattac ttctaacttt taaaatagga
    15421 tttggatttt tattataatc ttctttgtat tttcttcttt tcattattat ttcttttaac
    15481 aattctggaa caaatccttg ttcttttgaa aaatagacct tttcgttttg aaattctcca
    15541 acataagaat tttttttgtc ttctgtttga gttgattttg agagattata tgtaacaata
    15601 acacttccat acatgcttga gaaatcaaag aatgcaatgt tttcatataa tcccggagtg
    15661 ggttgaaaaa caaatgctcc ctgatatttt tctctgtttc ttctttcaat tatttctggc
    15721 ctgtaaggtt ttttttctgg tatttcattg aatctttcta agttgtgcaa aacataatct
    15781 tcaaaatttc ctgccattgt gcttcttgat acactaaaaa ttggctcttt tgttattttt
    15841 gttatttctt ctaaatctgc ccaaattttt tcaagaattt tgtaagttaa ttcagaatct
    15901 ttgagattat attcataaaa tttatcccaa tctactttat tttttctgga ctcttctaaa
    15961 ggacttaatt ctatttttct ttctccaagc aattcattag atacttcatt tagagaaaga
    16021 gtttctgatt ggagatattg agaatatgca ttttttatga atcgtaataa gtcaatatga
    16081 actattcctt ttatctttcc tgttaagaat attcctcttg aaaatatagg ttgtgaattg
    16141 tctaatccaa gatttaattt gatttttagt ttttctgttc ttgcctttat gtaaggtaag
    16201 tcaaatccat ctgagaaata tccaaccaat atatctggag agtatttttt tacttcattt
    16261 acaaatcctt caagcatttc tttttcatct ttgaattttt caacaaatgt tttttttgag
    16321 ggttctttaa atgtcaatac ttttttgaaa ttttctccat ataatgaaat cattaatatc
    16381 tctccttttc ctaattctaa ttccttagct tctatatcat atgcaagaat ttttggaaag
    16441 aattttgttt tatgtttatc ttctggtatt ttttttaaag attttaattc tatgcaaaaa
    16501 tcaacttcaa gggcagaatc tattcctccg aatttatcag aattatttag catttctcct
    16561 tcgatttcat accattctaa agggtttaat tttctttcaa ttatataatg tgtgataaat
    16621 cctaaatcat accctcttct tttttctatt ccttgaaagt ctaaattatc tgctaaatca
    16681 tgtaaatctt tgtaatttgt tgcgaatatt ttaagagctt caactgtttt tcctaaaaat
    16741 tttttatcgt ggatttcaat tttttctatc ctagtttttc tggatttttt atctaaactt
    16801 attttatcta cttcttctat taaatctcct aattcttttt tgcctatgtt atcttttaat
    16861 attgcccaga gataaacagg gcaggaatct attacacaaa ctttttttcc attttcatct
    16921 ctaccaaata cttgaatgta attttttcct tcataatcaa atggttgata atctaagggt
    16981 ataaaatttg ttttcatgaa tagattaaga gatagaatta tttaaagctt aggaatattc
    17041 atttttctat atttagtttt tacaaatgtt tgcatttgtt actacttata agatactata
    17101 tatagaaaag tttataaagt agaaggagtt taattaggta tggtaaacaa atatactgtt
    17161 tttaattatg ctccagaaac atatgagaaa gataaatatt tagttgatgc tatacatagg
    17221 agagtgcctt atttgagtag aggaagacct caagatgctt tagaattagc aaagattcta
    17281 aaagagtatg gttataaggg attaagagtt gcttctaatg attcacctaa tgcctttgtt
    17341 tctctggata aattagtaag tattaatcct tggagaatta ttagagttgc taatgatatg
    17401 tatgttcaag caaataaaag aatgagtttt tttggagatc catctaaaaa tcctgaaagc
    17461 cctttctata agaaaggacc tgatttagaa ggaagaattg aagatgatgg acaaggacgt
    17521 ttattttaga ttttagataa ttatttatat ttctgcagtt tagtttcaga tattttatat
    17581 tagaaatggg actaaacata agagaaataa tacctagaag ggaacttgaa gaatctgaac
    17641 ttaaagataa aattgtttgt gttgatgcat ttaatgctct ttaccaattt ttatcaagta
    17701 taaggcaacc tgatgggact ccacttatgg acaataaaaa aagaataact agccatttgt
    17761 caggtatctt ttatagaaat attaatctta tggaagaagg cattaaattg gtttatgttt
    17821 ttgatggtag agctccagat ttaaaatata aaacacataa agaaagagaa acttctaggg
    17881 atattgcaaa agaaagatat gaaatagcaa aaggtgagtt agatattgaa agtatgaaaa
    17941 ggtatagttc tcaattaaca agacttaata gagagatgat tgaagagagt aaagagttgt
    18001 tggaagcaat gggaatacct gtaatacaag cttcttctga aggagaagct caagcagcat
    18061 atatggcaag aacaatcccc agagtttatg catctgttag tcaggattat gatagtttac
    18121 tttttggagc tccaaggtta ataagaaatt taactgtttc aagaaagagg aaaacttata
    18181 gtggctatgt tgaaataaaa caagaaatta ttgaattaga taaagttctt aatattcttg
    18241 agataaatct agagcaatta atttgtttag gtattcttgt tggaacagat tataatccta
    18301 aaggtattcc gggtatagga cagaaaaaag cattggatat tgttaaaaaa tataaatttc
    18361 cttatgagat attcaagagc gttgaagaac aaattatgag tttgtcagaa gaagatagat
    18421 ttgactggca ggagatattt tctctttttt ataaacctaa ggtagacaat gttgattttg
    18481 aatttaaggg atttgatgaa aataaaatca gagagatttt agttgaaaga catgactttt
    18541 ctgaagaaag agttcaaaag caaattaaca gattaaagga tttgaaaaat aaaaaccaac
    18601 aaaaaagttt aggtaaatgg ttttaaattt atcttcttaa atatattctt ttatcaactc
    18661 ttctcttgaa ttctcttttt attaatacta ttccaataat taagaagatt attcctatta
    18721 ttctaaacca ccaaactaag ttgtgcaaga aaaagcttcc aactataaat aaaactccga
    18781 agaaagagaa aataaccctt gcagttactg ctaaaatcat gtatactagt ccaactaaaa
    18841 ttattattgg gataacactg tatagaagat tgttgtttat tcctattttg taaaaaaatc
    18901 ctgggaagtc aagatttgtt ttacttaaac catatactga caaaagagca atagataaag
    18961 agattactcc tagtggacct ttatctttta tagttttaga gagtgctaat cttattatag
    19021 caaagaaaag cacaaagatt agtccaaata tgattaactc tgcatctaag ttatagaaaa
    19081 tatcaacccc catatctaat atatggataa tctgtttata aatttatttt tataagtttt
    19141 aggaagattt aaatcctttt tttaatattg ttgtatatgg taaatcatcg ttcatttaat
    19201 attttgggaa atatagctgt agttaatttt ccttatggaa caaaactttc agataaaaag
    19261 aaatttgcat tagaaatatt aaataaaaat aaattcataa aaactgttgt ggaaaagtca
    19321 ggtaatttcg ctggcagact taggaagatg gaaacaaaac atcttgcagg agagaaaaac
    19381 aaggaagttt tgtataaaga aaataattgc tcttttagat ttaatttaga tacaacttat
    19441 ttttctccaa gattaagtaa tgagagaaaa gaaattgctt cttgtataaa aaataattcc
    19501 agtgttttag ttatgtttgc aggagttgca cctttttcca tagttatagc taaaaattct
    19561 aaacctaaag aggtttattc aaatgagata aatagagctg ctaataaata tggggagctt
    19621 aatgctgaaa taaatggagt taaaaataaa attaaatttg ttaatggaga tataaaaaaa
    19681 gtagcagtta aattaaagga agaaggtaaa aaatttgatt ttatagttat gccacgtcca
    19741 aatcttaagg agagcttttt agagcaagca tttatgttaa gcaaaaaagg aacaataatc
    19801 ttttattatg atttttgtaa ggtggatgaa gtagataaaa ttcttgaaaa gataaataag
    19861 aaggccttgg aatctggaaa tagaataaaa atattaaaca agaagcctgc aggagaaatt
    19921 gctccttata aaataagaat aagggttgat tttaaggtct tgtgattatt aaaaaatctt
    19981 ctccaaattt ttgtttgtat ttttggtagt tttcaattga attatctttt tttaatctgc
    20041 tgatattttt ttctaaagaa tatcctttaa aattttcttt agaaaattct tctagtgtct
    20101 tgtatccaag attaacatat aggggttttt ttggatttaa ttttaatcct tgtattttta
    20161 atatttgagc gagtggtaaa tcttgtcttc cattgtattt tccttctaat agatatactt
    20221 cattggctct tttgtaattg agaaaatatt cttttgattg tttatcattg ttttggaatt
    20281 ctataacgaa atgcaatctg ttgaaatcaa atccatttga aggaaggtgt tcttctatta
    20341 ctagtttatg gggtattttc ttttttgaaa ttaaatgcga agaaaccaag gttaaatcca
    20401 aacaaggatt cataaaacaa gtttctccac ttgataatat ttcttctact gagcgtctcc
    20461 caaaatattt atctatttct tcatttgtcc aatacactcg ttttaccttt ttgtttactt
    20521 ctgagattat ttcttttgct atgttaattg ctttatccat aaaaagatgt aaaacctttg
    20581 gtttaaaaat attattaaaa tttcttttct gcttttaatg cagtaggtgc ttctcctcca
    20641 tgccaagtaa atcttggtct tattctcata ggatatattc tttcagaatt atcatctaat
    20701 ataattgctg aaccatttag tgagggtaat tcatccatat attgtttaat actttcaaga
    20761 agataacttt gcattatctt attgagtgct tctaaatctg gtgcagaagt aactctatgc
    20821 gagattacta tatcggattg agtcattaca tctttgtgta tctgacctgg ttgttgagtt
    20881 gcaagaacca gagatattcc tggttgtctt ccttctctta ataattgtgt taatgcatca
    20941 cttgctattg tttttccctc taaaggtaag aattcgtgtg cttcatctat aaataaccag
    21001 attaagggat tttctttttt ttctacatag tagcccatat ctaatcctct tgatatagat
    21061 tttatttctt ctttttttct tgcagccatt ctttgattaa agagttttct tgatattaga
    21121 cttataacta atgctcttac attaaaactc cctacagaat tataaacact taaatctaaa
    21181 acagttgttg ttccagcatt tactaaatga gttatttttg taggtgaaat accttttttt
    21241 gagaatattc cccatgtgtt tgcagcttca aataaccctg ttgcagcgtt ttttatctct
    21301 ctgtctgttt tttggtcttt ttcaatttca tttactatat cgtctatatc aaagattttt
    21361 tttgttctca gtttgtttat tattctttgg atgagaacag caacaggatg gattatatct
    21421 aaaccaaatg ttaatatcca atcttcagca ttcatttcag ttacatctaa tgcaaacttt
    21481 tcatctgcag gtatacctct tcttacatag tcatcataaa acccaaatgg gatgaatatt
    21541 tttacattca aattttttgg ttttaaatcc cattcatgta aaagttcttt atctttttcg
    21601 tttgcatatt tcattgtcca gtaaattccc atagtgtcaa acataagaga cgcaatattt
    21661 tgtgaaacat ctttgggtaa attagacaat tcctctgcta taactcctag agtatagctt
    21721 ttaccgcttc ctcttttacc tgcaacaagt acaacatggc tccttattac atccatgaat
    21781 attttattac ttaaagaagt gtattgcccc atttttacaa acccctttcc aatataagct
    21841 aaaccctttc cctcaaattt ttttttatct gaggagtttc ttcctgttat aatttcatat
    21901 gacatttata aataagttta cattaatttt taattgtttg tttttgttgc aataaattaa
    21961 agaaccacaa tatttttaaa gcatttattc atcttttaaa aatggaaaat caacctaaag
    22021 aacatattgt tcatgttaat agagaaagaa gacatggtat aagagacaaa ccttgggttg
    22081 tttcaacaat tgctttaata atcttggttg ttttacttct ttcattgaat gtatttaata
    22141 ttactggaaa ggttgtttca gctaataaag ctggagagaa gcttgctgat tttgcagaat
    22201 cacaaggatt aacagttgaa gtttctagtg ttgaaaaaga aggtaatttt tatttagtta
    22261 ctcttttgat tgaaggtcaa gagttgccaa tgtatgttac taaagatgga aaatatttca
    22321 caaattatct atatccttta gaagaagagg aagaaaaatc taataataat caaaatacta
    22381 atactcaaac tcaagaaata cctaaatctg aaaaaccaac tgttgattta tttgttatga
    22441 gtcattgtcc atatggaact caagcagaaa aaggaataat ccctgctatt gaagctttag
    22501 gagatacagt taattttaac ttaagatttg tatactatat tatgcatcct tctgctgggg
    22561 aagttgaaga acagacaaga cagtattgta tacaaaaaga acaagaagct aagttacttc
    22621 catatttgaa atgtttctta ggaaaaacag gaactgctgc tgattcagaa gct
//
LOCUS       DAOPCH010000007        22341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_2470_length_22341_cov_4.598537, whole genome shotgun sequence.
ACCESSION   DAOPCH010000007 DAOPCH010000000
VERSION     DAOPCH010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 22341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22341
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2470_length_22341_cov_4.598537"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PLU55_00920"
     CDS             complement(<1..829)
                     /locus_tag="PLU55_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86654.1"
                     /translation="MGSVVSDLIEKISPNYKEGPAAKHTLVYDSAVETLEPVYFFIID
                     LMADMGLKTEKLIDNFSSSPGSGHFAELGQRATIMQNQASKIMGDINTVLRSVLNIVY
                     DLKEFRTRLQSYDDLKNPSKKEGAILSLKQIWMDKVDIQKGNSSIKAMALGQSGFVTL
                     IDAFLASKDENHAKSLDLNERVKRILIPRIAEFNIWLKQSEQELRKRYELERNYLKSQ
                     VNSLKLYSRWVKPYLRAAYDLESKENLREPALVKTFNTIMLNLTLIGKREINPKDAAM
                     "
     gene            939..1352
                     /locus_tag="PLU55_00925"
     CDS             939..1352
                     /locus_tag="PLU55_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86655.1"
                     /translation="MGLFRRNKIVDYTENYHYQRKKAKANMKDNLSENSDTSSASSSK
                     TQESSLGGFFNFFGGSSPSSQSTSNISDNLSTSDNSGLSSSTEILDAEEKRRRLALRL
                     KNMTDRIEDLSNQLYQLQQRMEVVEKKINLGRYEQ"
     gene            1349..2563
                     /locus_tag="PLU55_00930"
     CDS             1349..2563
                     /locus_tag="PLU55_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPJ86656.1"
                     /translation="MKDFEKKAKAHALKNAIRYNGKASSGAVISGLFAEGLKKEEVKE
                     YSKKINEIVVSVNKLSLDEQKKEYEKLQGIISEREVREGLEELPNVPKSGVVMRFAPA
                     ASGPMHLGHAITGTISSLYVKKYGGKFYLRIEDTNPDKVFSDCYKSFKEDSDWLFGNV
                     TEYIIQSDRMEIYYKYAEKFLRKGAAYICTCSPEKFKGFVNLKENCPCRNNDLKENLY
                     RWKKMLSKDSKENYKQGSAILRFKSSMQDSNPAMRDFPLARINENEHPRQKKKYRVWP
                     LMNLSVTVDDIEYGMTHIIRAKEHMDNAKRQKMMYKVLGLEKKFPWTYFMGRIKFNDL
                     PLSKRKIVAAIESGDYEGWEDIRLPTLFALKKRGYKSEAFFKFAEQRGLTDVDKVMDS
                     KDLFKILDTFNK"
     gene            2652..3155
                     /locus_tag="PLU55_00935"
     CDS             2652..3155
                     /locus_tag="PLU55_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86657.1"
                     /translation="MKSNKKGLSTIVATLLIILLTLVAVGIIWVVIRGVVRDSTDQVD
                     IDSKCLAANVEATRVVLDTTPDPDEYLVTITRTGGDDALGGVKLVFTASDGSSNAVID
                     WDLTGTGTHAPLPALGSRQISIPVGGGENQIPSDLNPTMVSVVVYFLDTSNNPQLCST
                     ANAYKFG"
     gene            complement(3245..4465)
                     /locus_tag="PLU55_00940"
     CDS             complement(3245..4465)
                     /locus_tag="PLU55_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPJ86658.1"
                     /translation="MQKNTKNKERYELLAPAGNFAMLQTAVNSGADAVYFGLKEFSLR
                     ANARNFQLKDLNKINEICNKNKKQGKIVKKYLTLNSIIYSDELKKIEEIIKKVKGKVD
                     AIICWDLGVIQLCKKYKIPFHISTQASISNIESAKFYEKLGAERIILARELNLKQIKE
                     IIKNIDIKVETFVHGAMCLAVSGRCLMSQFLFNKSANRGECIHPCRRSYTIKDTQEGY
                     ELEIENNHVLSAKDLCTLPFIEDLKKAGITCFKIEGRNRDPRYLDKVIQVYKKALNKK
                     LTPNEIKESMEELQKVYNKGFSTGFYLGKPTPKDFSPIEHSNATEKKQFIGKITNYYP
                     KIQVATIKLVSGIKVGDKIIVIGKSTGIINSKIERIEIKKSPVEKAEKGQEIGVKLPL
                     VRKNEEVYKIVPSK"
     gene            4572..4979
                     /locus_tag="PLU55_00945"
     CDS             4572..4979
                     /locus_tag="PLU55_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86659.1"
                     /translation="MKNLSKILGISLLGALSFNSHAQNKSFVKEDYHQYCMNYALADL
                     NGDSIYDVVAVDYDLNLNGKKDVRGLFIITGKREEPGQGVVYTTKNKAFMLVIDYNED
                     GEDDEILVDRDLDGNLDFYKNLKDIRKPKPLIL"
     gene            complement(5026..5355)
                     /locus_tag="PLU55_00950"
     CDS             complement(5026..5355)
                     /locus_tag="PLU55_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86660.1"
                     /translation="MNKKLVLLVGVMLALLVVGTAYAAGEKSASKEVYGTCVSKNAEV
                     KKTCFGVSKDALKKCKTSIPDGDRKKEAKKQCKLGYKKAKKQCKAGFKTGKEVCKKAR
                     DLAKEFS"
     gene            5527..6315
                     /locus_tag="PLU55_00955"
     CDS             5527..6315
                     /locus_tag="PLU55_00955"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2080 family transposase-associated protein"
                     /protein_id="HPJ86661.1"
                     /translation="MHNNMEEKIVRKVTEIGNGAHIFAPKEWLGEEVVIVRTPRKDPK
                     EEVIKVLYPYLDKIIAVFLFGSYARGEQEKNSDMDVFVISNEKFKVNSNNIDLIIVPE
                     NKLEIAKEINPILFYSMLNEAKQIINPSYLEKLKKEKVNFGYFKWFIEDTKKSIDSNK
                     EIIEMDKKLKNRGVSESVIYSLILRLRGVFIINLLLNNKKYSKKIFKEWVIKNSSINY
                     DDAYSIYQSVRDNKKPDKEVLMEQGETLLSLLIEETENLSKKIK"
     gene            6337..6522
                     /locus_tag="PLU55_00960"
     CDS             6337..6522
                     /locus_tag="PLU55_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86662.1"
                     /translation="MKRKKRLKKGIDSLKEQIEIHEKKKKKAEEADMKELVGYYEKEI
                     LSKEKALKEKKALLDKQ"
     gene            6635..7312
                     /locus_tag="PLU55_00965"
     CDS             6635..7312
                     /locus_tag="PLU55_00965"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HPJ86663.1"
                     /translation="MKRLSSSEIFLIVANLAPILGIFFFDWSLFSVLIAYWVETGVVF
                     LYALAKRIYTVRNSKRKEDRSEALASIFTAVAAYGSFIIIHFALILDLAYPGINNTLS
                     GVFKEYINEIGLMIVIFFISHGQSFYINFIKNRESEKIEVKELLFDDIKNVLNRFLLM
                     QLIVLAGFYLLEFTNGSIYLYAIFIFAKIVVDLNAHRRKHELESYKSFIPDPNTPKPL
                     YVPPNSY"
     gene            7388..7458
                     /locus_tag="PLU55_00970"
     tRNA            7388..7458
                     /locus_tag="PLU55_00970"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7420..7422,aa:His,seq:gtg)
     gene            7562..7840
                     /locus_tag="PLU55_00975"
     CDS             7562..7840
                     /locus_tag="PLU55_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86664.1"
                     /translation="MKNKRWILTLIIVLIILILGYFFYPLCRFHKIGSDFYINADSTP
                     TLTCHCSISGAVCVSEGKHVDLIEGEYIDLTEDELKYRVDDAFNEDNL"
     gene            7953..8420
                     /locus_tag="PLU55_00980"
     CDS             7953..8420
                     /locus_tag="PLU55_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HPJ86665.1"
                     /translation="MGINGRLTNKNLERKIKDMEVGEEGFAVPWALGFNEYKIPYLNV
                     EFSIGKSPYGSRSMPIKRIGRGLADYDINLEFDYFGGAYKWDTSSEPFEGVIGVNPDS
                     IVMLNYNSQISDKDRYPKTYEIRQKLTEAINKEDYELASKLRDEINKTDEPLK"
     gene            8531..9448
                     /locus_tag="PLU55_00985"
     CDS             8531..9448
                     /locus_tag="PLU55_00985"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPJ86666.1"
                     /translation="MKQYDIAIIGAGPAGLTAALYSARNGLSVGVISKDIGGTVNSVL
                     RIENWPGFSGSGPELIKKFYEQVKRYKIDFILEEVASIEHKDNKGKNKEFIVKTKQQE
                     IKCKSVILATGAEKKKLKIPGEAEFLGRGVSYCATCDSFFFKNKDVAIISESDCTATS
                     ALVLSDIAKKVYLIYRGEKLKCEDVNQEKLKEKKNVKIFYNTTPIEITGKNKVEELKI
                     FCKGKRDVLKVDGVFIEIGSAPLAEFTKNLGLKMDNEKFIRVDEDMKTSVEGVYAAGD
                     VTHTKLKQVVVAASQGAIAANSAYNWLNK"
     gene            9493..9678
                     /locus_tag="PLU55_00990"
     CDS             9493..9678
                     /locus_tag="PLU55_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86667.1"
                     /translation="MATSIEQFTAPGKYYYELSKENIEAQLRIPVNNICTYYTLGNKP
                     KSFRENTYYNSFKSRKK"
     gene            9730..10716
                     /gene="dph2"
                     /locus_tag="PLU55_00995"
     CDS             9730..10716
                     /gene="dph2"
                     /locus_tag="PLU55_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HPJ86668.1"
                     /translation="MAFNFETEKLRKHLEKIKPKRVLVQLPEGVKQNSFELLEFFEKL
                     KIEAIFSGETCWGGCAVAVEEAKSVKADLIVHFGHSEFMKVNFPVIYIPIKDELNLES
                     LLKKSLSELKFFKNIGLSCSVQHIHDLKNILEFYENNGKKVIVSKKKGNVSGEGQVVG
                     CQYFGLKEIQDKVDCFVVVGNNFHSMGAALAVDKPVFLIDVYNNEIKNMNGVKDKIIR
                     QRILSVEKFKDAKKIGIIIGVKQGQQFGSYKYLKDRFIKLGKEVVVLTINEFSPDKLV
                     NFYNIDGFVELACPRIATDDFARYKKPIITFKEALVVVGEKSWDDLIKEGLL"
     gene            10781..11254
                     /locus_tag="PLU55_01000"
     CDS             10781..11254
                     /locus_tag="PLU55_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86669.1"
                     /translation="MNILFVCKYNRFRSRVAEAYFNQINTKKNIKAFSRGVIRGDYPL
                     NRTEVAISKKCGLDISGKPKGLEIELLKKIDLIVIVADNVPKEVFYTTFKGRIIHWRI
                     RDIEHGDGKDLIERKVKRIMAKVRKLLKILDKESFIRKIQFQKKKAREDHLNRRK"
     gene            11271..12440
                     /locus_tag="PLU55_01005"
     CDS             11271..12440
                     /locus_tag="PLU55_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HPJ86670.1"
                     /translation="METVRGFKDYTGEEARKRAEIRKILVDTFEKYNFEPVETPIVEY
                     REFVQGDNKQDEAVSDIFKLKDKGGRDLALRYEFTFQLKRLMKGKKLPYKRYQIGEVF
                     RDEPVSSNRFRQFVQCDIDVIGSKIRDEVDILSLAKEALNKMGIKSVIYFNNRKLLNE
                     ILDKEGVSEKDKVFVIKEIDKLDKLSEKEIKENLKKYNAEKILEVFKKPENYFSKYDS
                     YKDVKEFVDYLSLYKVEAKFSPSLARGLSYYNGTVFEIKTDKMKDTICGGGSYEFNGI
                     QCTGISFGLDRLAILADFDFNKNNFLVVSLDEDRQAIKIAEMLRQRGDAVSIYYGKPS
                     KALEYANSCNIKQVIFVGAQEVKSKKFKVKDMVTGKEKAITLGKITKKNVILKRK"
     gene            12455..14140
                     /locus_tag="PLU55_01010"
     CDS             12455..14140
                     /locus_tag="PLU55_01010"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPJ86671.1"
                     /translation="MGKFKGVGVNPELPLVLTITFLLFLIGFLLLFFQGNPDFNFYSS
                     KIVIDGQNITEELHFKPDKDYHTLYRNFENKVYFFHHENIYSKNYFLIKSVDCSHGNY
                     YFKDYDGVPYPKSYPYTENNEYGCTFGYDTGFLKNQEYTIGAEYEIHPENLFKIGNNY
                     YIKFVAYSPKNHVKLTNKNFEVYGDVVKDDKYLADQNVILYIPYSGEISGFNIIEAKD
                     FEFDSNFKYKIKYLIFFFPSIFFFIVWFIFGKENTYESIPKELSSFPNKRVFWEVATY
                     FNPPFSKIDKNFFAATLLNFYNKKIIDIREKDKEIYIKLNKFEGDDLERKIYNLLGEA
                     ERLLKDKANKGLLLGGLTSKQKQKSFDGEYFNLKMSMMDSLLRTDFSEIRNYVQSEGK
                     KYIHNNSGIVILVLTLSFSFAFILQYKLNIYIDFLDLIYVLFFVVFLFTIPLTRGTLF
                     SKYKGNYYVEYQKWKAFKRYLKNSFSIKTATHKTVVMWNEYLVYATALGVPNRVIKEL
                     QANNLIDEKQVNMYNGVILASSASFSSSSGGAGGGGFGGAGGGGAGGGGGGGR"
     gene            14189..14920
                     /locus_tag="PLU55_01015"
     CDS             14189..14920
                     /locus_tag="PLU55_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86672.1"
                     /translation="MNKRGQVTIFIILAILIVASVVLFFVFRDNLGIEETKDIQSSLV
                     VNFVEGCMDKTLNNSIYAVAANGGYSGYSYFSRQSTESGVRYYFFRESNYMPSKSLVE
                     KQIEEYFKRKFFICINDFSDFNGYSVEEGLLKVSVRIKEEKVVLEADYPLTIVQGKNK
                     VRVRDFKSEIPSRLNIIYSSVKYFIDNHKNRKSLCLGCLKMAIENDIYIDMESSYDGT
                     VVFIFRDNSPELNSKPIEWVFANRY"
     gene            14922..17933
                     /locus_tag="PLU55_01020"
     CDS             14922..17933
                     /locus_tag="PLU55_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86673.1"
                     /translation="MDKKKLLLFVGIFLFLILGLSVLSTEEVNKNGINIIKDFGNLFM
                     NLPEGSIIGSEDGVKYDRLKEGGRFTFTGEESSLIIQGSSFEGVLPKDSDGHSSFIEV
                     NLLGNITKADFAVGENGGEYLIGGVKFDLPPNSRVYYDNENGFRFPEGTKITSLSEFY
                     NLGRELQFEGNNLNFIDEVIVNGQLKLNKYGYLIGKGEATYEGMKIDNFFGHSDVAIL
                     KNNLDKLKEEDISELSWIKKDSNGLEFSSSSTGSIFVEFLEGNDILNVGKHGKLELRI
                     DGGDKLKISKFSGVPRFNYIPSENGFTNIINDGLSLELDKGGKHYVDISPVTLKDFSL
                     DKYAAVPAIIESDVLDEEIRINSRGTLYGVLVDNTGGKKVEEIVYDLENPIMSLKTEE
                     LNGLMGSLKENIKEEGNLDSAFRASQFFLLSAKNNHFTTSENIDLAKKIVHSSGEYSD
                     SVLYKVKFGLDKLSKDDVKISYEKFSQFTLKNLDNLDFKYGDGLDKLDAVYSVLNNGL
                     KENKDFNLIEDFSLKIIKSDFNSDKSILLLSKNINEYNGANFESMKLGAEVVGRYNMV
                     PGVVDFLDLKYSNIKDKKTFGKEYALALNQYFKDKDSIQKSDSQMASFTLDQMHLLES
                     QIEHSDIIRNSIISNLDANSKYYLIANSEGNNNLFTSTFEKIYSSIDKKDVSELMKSV
                     DPNGDYWLKFTTELSARGKLPELYKQDTVFYRKLIQDSFDNHDEDILFENAVLMTETF
                     QDLYNRPDIYPGEKEAFEEFLISKYKSSESNNEKGVYAYLLKLNTNPNTQKAEKIIND
                     LPELRDAILPSSFSQRDEIAAKLYFYSDENWFSIATNDFNSNYKMDLEWNNGRQARLS
                     KPINGKKIIIDLELNDPSVDFVPSIEEDIKGQTYLYIAHRGHSFNTEKTYPDFLSSDT
                     TKIIHLAGCGSHGALADYQKKFPNSFFIADKDAGEGFPSDKVAYFVLEGIAEGKLNWE
                     DLTPDFAEEKGLVFPEQESLPQYLKRYESEM"
     gene            complement(17936..18598)
                     /locus_tag="PLU55_01025"
     CDS             complement(17936..18598)
                     /locus_tag="PLU55_01025"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPJ86674.1"
                     /translation="MEKLKKISIATAAICGLIIALSPHKTIPITNKKLQAISSLENII
                     DTNKTISINSIKLDSSDNFKELIKYNLWVDSTLNESKANNSNSIIVNKERRKLYLVKN
                     GKIDSEYNVDLGFNPYADKQAEGDGCTPEGMYIVEKKLPPGSTNFYKAFLINYPNKED
                     KAKGKTGGLIEIHGYGGKGYDWTLGCISPSNEDMDKIFPYIEKGDRITIIKRTTKELS
                     KQ"
     gene            complement(18744..19511)
                     /locus_tag="PLU55_01030"
     CDS             complement(18744..19511)
                     /locus_tag="PLU55_01030"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPJ86675.1"
                     /translation="MGKNNAIIITSIIAGVVLVIALLTLSTFNSVVPSSKNSVTVQGS
                     STIKAMPDLVSVYITIKTNGTTSAEATSENTKIYDKLVEEMVIAGFNKNDLKTESFSV
                     YEHTYWDYKEKKSKTEGYIANHYIRVELTSDNMNKVSKVVDAAVKAKTEISYINFELS
                     PESQKRYKAQALKEASEDATIKAESIASGFNKKVGKLLSVETSDFGYSPWNVYASRNT
                     DGVYMEEIAKEATQAATTIQPGEEKISASVYATFQIK"
     gene            complement(19559..20197)
                     /locus_tag="PLU55_01035"
     CDS             complement(19559..20197)
                     /locus_tag="PLU55_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPJ86676.1"
                     /translation="MDENHFMLISLEDSKSKAISEVLGSKTCKKIIAYLSERKEASQK
                     DLSDALNIPMNTLDYNIKKLVESGFVQKKKEFFWSKKGKKIIMYELSNKSIIISPKKS
                     TSQKFKSIIPAFILTAAGTFAVWAYEKIKYTTNYTQEIIQQPVMDCVERNSHELLSPE
                     SLSKGADVVCSTIASSDYTSLTASASPLIWPWFLAGALLVILIFSIVNWKKI"
     gene            complement(20240..21442)
                     /locus_tag="PLU55_01040"
     CDS             complement(20240..21442)
                     /locus_tag="PLU55_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86677.1"
                     /translation="MGILNLLKNIFKEAEEKEPKRIKVNLSEIKPILDKNKEKIQERE
                     DSIISLIKTNIDSFSKDIQEKVEIVEKVDIESKEKNNKIKAIVYEGRKKYIEFIERLM
                     DNLEEHKNIDNFQEIIENINYSFSKFNESSMKSYERATVLIGKEMGAIKESLKKFSKE
                     ILEIYNENKNIINEKKSILFSESKLNENLEIQDKLNKIDEESNELKTKIKEKENILNE
                     KLTILNKIKNSEEYIKNIQLKESIIIKEEEIKNQISQLRQDIDFKSLSSFFHIFPEKM
                     NYIKRYKESFLLEFKEDKGKSLVNLLNEAGLNNDKISEQIKNISMKEEILEKQKKEIK
                     EDPTNLVSSEIETLKENIKNWNDELDWIEKRKEKLKEDEKRNTQKIKEEFKKINIDIE
                     EKDNFKQI"
     gene            complement(21522..22190)
                     /locus_tag="PLU55_01045"
     CDS             complement(21522..22190)
                     /locus_tag="PLU55_01045"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HPJ86678.1"
                     /translation="MKLASLFSGGKDSVYSIYLAKNQGNQISCLITLCSENKESYMFH
                     TPSIKKTKHQAKSMDIPLIIQKTKGKKEEELKDLKKAIIKAIKKYKIQGVVTGVLYSE
                     YQNSRIQKICDELNIKCINPLWHKEEIPYLKELLRENFKVIITGVFAYPLNQSWLGRN
                     IDEKFIEEAKILNQKYKIHPAGEGGEFETFVLDCPLFKTPLKIKSFKDFKEGENSWRR
                     EIII"
BASE COUNT         7616 a         2572 c         3645 g         8508 t
ORIGIN      
        1 ccatagcagc atcttttggg tttatttctc tttttccaat taaagttagg ttcaacatta
       61 ttgtgttaaa tgttttaact aatgcaggtt ctcttaaatt ttccttgctt tctaaatcat
      121 atgctgctct taagtaaggt tttacccatc ttgaatataa ctttaaacta tttacctgac
      181 tcttcaaata atttctttca agttcatatc tttttcttag ttcttgttct gattgtttta
      241 gccatatatt aaattcagct attcttggaa ttagtattct ttttactctt tcatttaaat
      301 ccaaggattt agcatgattt tcatctttgg aagctaagaa tgcatctatt agagttacaa
      361 atccactctg tcccaatgcc attgccttta tacttgaatt tcctttttga atgtctactt
      421 tatccatcca tatttgttta agagaaagaa tagctccttc tttttttgaa ggatttttta
      481 aatcatcata gctttgtaac cttgttctaa attcttttaa atcataaact atattgagaa
      541 cacttcttaa aacagtgttt atatccccca ttattttgct tgcttgattt tgcattattg
      601 ttgctctttg tcctaattca gcaaaatgtc ctgaaccagg tgatgaagaa aagttatcta
      661 ttagtttttc agtttttaat cccatatctg ccattaaatc aattataaaa aagtatactg
      721 gctctaatgt ttctacagca gaatcatata caagggtatg ttttgctgca ggaccctctt
      781 tgtaatttgg ggatattttt tctatcaaat ctgatactac tgaacccatg taaatctaaa
      841 gaatttccca tttaaaaaat gtttgatgaa ttatttaaat acttttttga ttaactgtta
      901 ataataactt ttaaaaataa tctttgtctt tattgattat gggtcttttt agaagaaaca
      961 agattgtgga ttacactgaa aattatcatt atcagagaaa gaaagctaag gcaaatatga
     1021 aagataatct ttctgagaat tcagatactt cttctgcttc ttctagtaaa actcaagaaa
     1081 gttctttagg aggttttttt aatttttttg gaggttcatc tcctagttct caatctactt
     1141 caaatatttc agataatctt tctactagtg ataattcagg attaagttct tcaacagaaa
     1201 ttttagatgc tgaagaaaaa agaagaagac ttgcactaag acttaagaat atgacagata
     1261 ggatagagga tttatccaat caattatatc aattacaaca aagaatggaa gttgtagaga
     1321 aaaaaattaa tttggggaga tatgaacaat gaaagatttt gaaaaaaaag caaaagcaca
     1381 tgcacttaaa aatgcaatcc gttataatgg aaaggcaagt tctggagcag ttatttctgg
     1441 tttgtttgca gagggtttga aaaaagaaga agttaaagaa tattctaaaa aaataaatga
     1501 gattgttgtt tctgtaaata aattatcttt ggatgaacag aaaaaagaat atgaaaagtt
     1561 acaaggtatt atttctgaga gagaagtaag agaaggatta gaagaacttc caaatgttcc
     1621 taaatcaggg gtagttatga gatttgcacc tgcagcttct ggaccaatgc atttaggaca
     1681 tgctattact ggaacgatta gttctttata tgttaaaaaa tatggaggta aattttattt
     1741 aagaatagaa gatacaaatc ctgataaagt ttttagtgat tgttataaat cctttaagga
     1801 agattctgac tggctttttg gtaatgtaac cgaatatata attcaatcag atagaatgga
     1861 aatttattat aagtatgctg aaaagttttt gagaaaaggt gctgcttata tttgtacttg
     1921 ttctcctgaa aagttcaaag gatttgttaa tttaaaagaa aattgtccat gtagaaataa
     1981 tgatttgaaa gagaatcttt atcgttggaa aaaaatgcta tctaaagatt ccaaagagaa
     2041 ttacaaacaa gggagtgcaa tattaagatt taaatcttct atgcaagatt caaatcctgc
     2101 aatgagggat tttccattag cgaggataaa tgagaatgaa cacccgaggc agaaaaagaa
     2161 atatagagtc tggcctctta tgaatttatc tgttacagtg gatgatattg aatatggaat
     2221 gactcatatt atacgtgcta aagaacatat ggataatgca aagagacaaa agatgatgta
     2281 taaggtttta ggtttagaaa aaaaattccc ttggacatat tttatgggaa gaataaaatt
     2341 taatgatttg cctctgtcta aaagaaaaat agtagctgca attgaatcag gagattatga
     2401 aggatgggaa gatataaggc ttcctacact ttttgctctt aaaaagagag gatataaatc
     2461 agaagctttt tttaaatttg cagaacaaag gggcttaaca gatgtagata aagtgatgga
     2521 tagcaaggat ctttttaaga ttcttgatac ttttaataaa tagttcttaa acaaaaaggt
     2581 ttttataggt gttttttctt attaatgtag accataaatg gtcattttaa attgtttgaa
     2641 aggaggttga tatgaaaagc aataaaaaag gtttgtctac aattgttgca acactattaa
     2701 tcatattgct tacacttgtg gcagttggta taatttgggt tgttataaga ggtgttgttc
     2761 gagatagcac cgaccaagta gatattgact caaagtgttt agctgctaat gttgaagcta
     2821 caagagttgt tttagatact actcctgatc cagatgaata tcttgtaact attacaagaa
     2881 ctgggggtga cgatgcatta ggtggagtta aattagtctt tactgcttca gatggttcta
     2941 gtaatgcagt tattgattgg gatttaacag ggacaggtac ccatgctcct ttacctgctt
     3001 taggatctag acagatttct ataccagtcg gaggtggaga gaaccagata cctagtgatt
     3061 taaatccaac aatggttagt gttgtagtgt atttcttaga tacatctaac aatcctcaac
     3121 tttgtagtac tgcaaacgcg tataaatttg gataaatttt tatttttttt atttttcttt
     3181 tttaattatt ttatttttaa attagtgaat actttattta ttttatatag aaaataaatt
     3241 agaattattt agaaggaact attttgtaga cttcttcatt ttttcttaca agtggaagtt
     3301 ttaccccaat ttcctggccc ttttctgctt tttcaactgg agattttttt atttcaattc
     3361 tttctatttt tgaattaatt atccctgttg attttccaat aacaattatt ttatctccta
     3421 ctttaattcc agaaacaagt ttaattgtgg caacttgtat ttttggataa tagttggtta
     3481 tctttccaat aaattgtttt ttttctgttg cattagaatg ttctattgga gaaaaatctt
     3541 ttggagttgg ttttcccaaa taaaatccag tagaaaatcc tttgttgtat actttttgca
     3601 attcttccat tgactctttt atttcattag gtgttaattt tttgtttaat gcttttttgt
     3661 atacttgtat aactttgtca aggtatcgtg ggtcacgatt tctcccttct attttgaaac
     3721 atgttattcc tgcttttttt aaatcttcaa taaatggaag tgtgcataaa tcttttgcag
     3781 aaagaacatg gttgttttca atttcaagtt cataaccctc ttgtgtgtct tttattgtat
     3841 aacttcttct acagggatgg atacattctc cacgatttgc agatttgtta aataagaatt
     3901 gggacattaa acatcttcca cttacagcta ggcacattgc tccatgaaca aatgtttcaa
     3961 cctttatgtc tatatttttt attatttctt ttatttgttt aagatttaac tctcttgcaa
     4021 ggattatcct ttcagctcct agtttttcat agaactttgc agattcaata ttggatattg
     4081 atgcttgagt tgaaatatgg aaaggtattt tgtatttttt acatagttga attacgccca
     4141 aatcccaaca gattattgca tctacttttc cttttacctt ttttattatt tcttctattt
     4201 tttttaattc atctgaataa ataattgaat ttaatgttaa gtattttttt actatttttc
     4261 cttgtttttt atttttgttg cagatttcgt ttattttgtt taaatctttt aattggaaat
     4321 ttcttgcatt tgctcttaat gaaaattctt ttaatccgaa ataaaccgca tctgctcccg
     4381 aattaacagc agtttgtagc attgcaaaat ttcctgctgg ggctagaagt tcatatcttt
     4441 ctttgttttt agtgtttttt tgcatattat cagaaagtaa taagagtttt taagagtgat
     4501 gatttgtttg gagtatctta tatcttgttt tgtataaata cttaaaaatc ttttaaatct
     4561 taaatcgttc tatgaaaaat ttatcaaaaa tactcggaat ctctttatta ggagctttga
     4621 gttttaattc tcatgctcag aataaatctt ttgttaaaga agattatcat caatattgta
     4681 tgaattatgc tttagcagac cttaatggag attctattta tgatgttgtt gcagttgatt
     4741 atgatttaaa tttaaatgga aaaaaagatg ttagaggttt atttataatt acaggcaaga
     4801 gagaagaacc tgggcagggg gttgtttata caaccaaaaa taaggctttc atgttagtga
     4861 tagattacaa tgaagatgga gaagatgatg aaatattagt agatagagat ttagatggga
     4921 atttggattt ttataaaaat cttaaagata ttaggaaacc taaacctttg attttgtgat
     4981 ttttattgag ttaaaataat aaaaaaataa aataaaattt tgtttttaac taaactcttt
     5041 agctaagtct ctcgcttttt tacatacttc ttttcctgtt ttgaaacctg ccttacattg
     5101 tttttttgct tttttatatc ctagtttgca ttgttttttt gcttcttttt ttctatcacc
     5161 atctggtatt gatgttttac attttttcaa tgcgtcttta gatacaccaa aacaagtctt
     5221 ttttacctct gcatttttag acacacaggt accatacact tcttttgatg cagatttttc
     5281 acctgcagca taggcagttc ccacaacaag taatgcaagc attactccaa ctaagagaac
     5341 taatttttta ttcatcttat aatcctccga tataatgaca tttagtatgt ttataaaata
     5401 tatgttttac accatatatg tttttttaac aaataaaaag aattatttat ttctttttga
     5461 gattcttctt tattcttttc gaaaattatc tttcaaaaca tttaaatatt agtatataca
     5521 tggtatatac ataataacat ggaagaaaaa atagtaagga aggtaactga aataggaaat
     5581 ggggcacata tatttgcacc aaaagaatgg cttggagaag aagttgtaat agttagaact
     5641 ccaagaaaag atcctaagga agaagtaatt aaggttcttt atccgtatct tgacaagatt
     5701 atagctgttt ttttatttgg aagttatgca agaggggagc aggaaaagaa ttcagatatg
     5761 gatgtttttg ttatttctaa tgaaaagttt aaagttaatt caaacaatat tgatttaatt
     5821 attgtacctg aaaataaact tgaaattgca aaggagatta atcctatatt attttattca
     5881 atgttaaatg aagcaaagca aataattaat ccttcttatc tggaaaaact gaaaaaagaa
     5941 aaagttaatt ttggttattt taaatggttt atagaagata caaagaaatc aatagattct
     6001 aataaagaaa taatagaaat ggataagaag cttaaaaata gaggagtctc tgagagtgtt
     6061 atttattcac ttatattaag acttagagga gttttcatta ttaatcttct tttaaacaat
     6121 aaaaaatatt cgaagaaaat atttaaagaa tgggtcatta aaaattcaag tataaattat
     6181 gatgatgctt atagtattta tcaatctgta agagataata aaaaaccaga taaagaggtg
     6241 ttaatggaac aaggagaaac tttgctttca ttacttatag aagaaacaga gaatctttca
     6301 aaaaagataa aataaacaag aagagttatc tttcaaatga aaaggaaaaa aagattaaag
     6361 aaaggaattg attctttaaa agagcagatt gagattcatg aaaaaaagaa aaagaaggca
     6421 gaagaggcag atatgaaaga attagttggt tattatgaaa aagagatact ctctaaagag
     6481 aaagctttga aagaaaagaa agctcttcta gataaacaat aatttttgta aacaaaaaga
     6541 attaaaagtc cttcaattag catctatact ggtgattata gtatggtgtt ttctaaaaca
     6601 ataaattaat aaaatataaa ttgaattatt taatatgaaa agattaagtt cttctgagat
     6661 attcttaatt gttgctaatc ttgcgcctat tttgggtatt ttcttttttg actggagttt
     6721 attttcagtt ttaattgctt attgggtaga gacaggggtt gtttttttat atgctctggc
     6781 aaaaagaatt tatactgtaa gaaatagcaa gagaaaagaa gatcgttctg aagcactggc
     6841 atctattttt actgctgttg ctgcttatgg ctcttttatt ataattcatt ttgcacttat
     6901 ccttgattta gcatatcctg gaataaacaa tactttatct ggagtgttta aggaatatat
     6961 aaatgagatt ggtttaatga ttgtaatttt ctttataagt catggacagt ctttttatat
     7021 taattttata aaaaatagag aatcagaaaa gattgaagta aaagaattgt tatttgatga
     7081 tataaaaaat gttttaaata gatttttgct tatgcaatta atagttttgg caggttttta
     7141 tttacttgaa tttactaatg gctctattta tttatatgca atatttatat ttgcaaagat
     7201 agtagttgat ttaaatgccc atagaagaaa acatgaactt gaatcttata aaagttttat
     7261 acctgacccc aatactccta aacctttgta tgttcctcct aattcatatt aacctaaaca
     7321 aaaagaatta aaagtcctct aaattagtat ttttagtagc tgtcttatgg cagttatgaa
     7381 ttccatggcg gttgtagtat aacggtagta catacggttg tggtccgtat agagagggtt
     7441 cgattccctc catccgccct gtacaatgat gctcggttta tgtaactgta tgagtcagag
     7501 ctttcaatat tataaaaatt taaatgacaa aatatttaaa ttataaaaaa ttgattaaat
     7561 aatgaaaaat aagaggtgga tattaactct aataattgtg ttgattatac ttattttggg
     7621 atatttcttt taccctttgt gtcgttttca taaaataggt tctgattttt acattaatgc
     7681 agattcgact ccaacattaa cttgtcattg ttctatttca ggagctgtgt gtgtttcaga
     7741 agggaagcat gttgatttaa ttgaagggga gtatattgat ttaactgaag atgagcttaa
     7801 atacagagta gatgatgcat ttaatgaaga taatctctaa ttaatttttt atattcttca
     7861 ataatctcat atcttctcca tccgctattt tttgttattc tatgatagta acaaatagga
     7921 agatttataa ataggggttt ctttagataa atatgggaat aaatggaaga cttactaata
     7981 aaaatctaga aagaaaaatt aaggatatgg aagtcggaga agaaggattt gctgttccat
     8041 gggctttagg ttttaatgaa tataaaattc cttatttgaa tgttgaattt agtattggga
     8101 aatctcctta tggaagtaga tctatgccta taaagagaat tggaagagga ttagcagatt
     8161 atgatattaa tctagagttt gattattttg gaggagctta taaatgggat acaagttcag
     8221 aaccttttga aggggtaatt ggagttaatc cagacagcat cgtgatgttg aattataaca
     8281 gtcaaatttc agataaagat aggtatccga aaacttatga aattaggcag aaattaacag
     8341 aagctattaa taaagaagat tatgaacttg catctaaact aagagatgaa ataaacaaaa
     8401 cagacgaacc tcttaagtaa aacaactctg aaaataagtt ttttatctgt cctaaataaa
     8461 ccaatgaaac tcctttagat aattttatta attatattaa ttatatttta attagataat
     8521 ggggattaaa atgaagcaat atgatattgc aattataggt gcaggtcctg ctggtttaac
     8581 tgctgcatta tattctgcaa gaaatggatt aagtgtggga gttatatcta aagatatagg
     8641 tggaactgtg aattctgttc ttagaattga gaactggcca ggatttagtg gttctggtcc
     8701 tgaattaata aagaaatttt atgagcaagt aaagagatat aaaattgatt ttatacttga
     8761 agaagttgct tctattgagc ataaagataa taaagggaaa aataaagaat ttattgttaa
     8821 gacaaaacaa caagagatta aatgcaagtc tgttattttg gccacaggag ctgaaaaaaa
     8881 gaaacttaaa atacccggtg aagcagaatt tttgggtaga ggtgtgagtt attgtgcaac
     8941 atgtgattca tttttcttta aaaataaaga tgttgcaatt ataagtgaga gtgattgcac
     9001 agcaacttca gcacttgttt tgtctgatat tgcaaaaaaa gtttatctga tttatagagg
     9061 agaaaaactt aaatgtgaag atgtaaatca agaaaaactt aaagaaaaaa agaatgttaa
     9121 aatattttat aatactactc ctattgaaat tactgggaaa aataaagtag aagaattaaa
     9181 gattttttgc aaaggtaaaa gagatgttct taaggtagat ggagttttta ttgagatagg
     9241 ttctgctcct cttgcagagt ttacaaagaa tcttggtttg aagatggata atgagaaatt
     9301 tattcgtgtt gatgaagata tgaaaacttc tgttgaagga gtatatgctg ctggagatgt
     9361 tacacacact aaattgaagc aagtagttgt tgctgcttct caaggagcaa ttgcagctaa
     9421 ttctgcttat aactggttga ataagtagtt tatcataatt aaatttaaaa ataataaaat
     9481 aaactaattt ttatggcaac atcaattgaa caatttacag ctccaggaaa atattactat
     9541 gaattatcta aagaaaatat tgaagcacaa ttaaggattc ctgttaataa tatttgtacc
     9601 tattatacat tggggaacaa accaaaatct tttcgtgaaa atacatacta taattctttt
     9661 aaatctagaa aaaagtgaat ttttactcat ttaaataagt cttttaaact agctatttct
     9721 ttgataatta tggcatttaa ttttgagact gaaaaactga gaaaacatct tgaaaaaata
     9781 aaacccaaaa gggttttagt tcagttacct gaaggggtaa aacaaaattc ttttgaatta
     9841 cttgaatttt ttgaaaaatt aaaaatagaa gctatatttt ctggtgaaac ttgttgggga
     9901 ggttgcgcag ttgcagtaga agaagctaaa tctgttaaag cagatttaat tgttcatttt
     9961 ggacactctg aatttatgaa agttaatttt cctgttattt atattccaat aaaagacgaa
    10021 ttaaatttag agtctttact taagaaatct ctttctgaat tgaaattttt taaaaatatt
    10081 ggattgagtt gttcagttca acatattcat gatttaaaaa atatccttga attttatgag
    10141 aataatggga agaaagtaat tgtttcaaaa aagaaaggaa atgtttcagg tgagggacag
    10201 gttgtgggat gtcagtattt tggattaaaa gagattcaag ataaagtaga ttgctttgtt
    10261 gttgttggga ataattttca ttctatgggt gcagctcttg cagtggataa acccgttttt
    10321 ttaattgatg tttacaacaa tgaaattaag aatatgaatg gagttaagga taaaataatt
    10381 agacagagaa ttttatctgt tgaaaaattt aaggatgcta aaaagattgg gattattatt
    10441 ggagttaaac aaggacagca atttggttca tataaatatc taaaagatag gttcattaaa
    10501 ttgggtaaag aagttgttgt tttaacaatt aatgagttct ctccagataa acttgttaat
    10561 ttttataata ttgatgggtt tgtggaatta gcttgtccaa gaattgcaac agatgatttt
    10621 gcaagataca aaaaaccaat aataactttt aaagaagccc ttgttgttgt aggtgaaaaa
    10681 tcttgggatg atttaataaa agaaggatta ttgtaatttt tatcaagtgt aaagtttttt
    10741 ataggaatat ttcataatta aattatgaaa agaggtaaaa atgaatatac tttttgtttg
    10801 taaatataat agatttagaa gcagggtagc tgaagcttat tttaatcaga taaatacaaa
    10861 aaagaatatt aaagcattta gtagaggggt tattagaggg gattatcctc tcaatagaac
    10921 agaagttgct atttctaaaa aatgtggttt agatatttct ggaaaaccaa aaggtttgga
    10981 aatagagctt ttgaaaaaaa tagatttaat tgttattgtt gcagataatg ttcctaaaga
    11041 agtgttttat actacattta aaggaagaat tattcattgg agaataaggg atatagaaca
    11101 tggagatgga aaagatttaa tagaaagaaa ggttaagaga ataatggcta aagtcagaaa
    11161 acttttgaaa atattagata aagaatcttt cataagaaag atacaatttc aaaagaaaaa
    11221 agccagagaa gaccatttaa acagaagaaa gtaaaaacta ggttattaaa atggaaacag
    11281 taagaggatt taaagattat acaggagaag aagctcgcaa aagagcagag ataaggaaaa
    11341 tactagtgga tacttttgaa aaatataatt ttgaacctgt agagacacct atagtagaat
    11401 acagagaatt tgttcaagga gacaataaac aagatgaggc tgtttcagat atatttaagc
    11461 ttaaagataa aggggggaga gatttagcat taagatatga atttactttt cagcttaaaa
    11521 gattgatgaa aggtaaaaaa cttccatata aaaggtatca aataggagag gttttcagag
    11581 atgaaccagt tagttcaaat aggtttagac agtttgtgca atgtgatata gatgttatag
    11641 gtagtaagat aagagatgaa gttgatatac tttctcttgc aaaagaagca ttaaataaaa
    11701 tgggtataaa atctgttatt tattttaata ataggaagtt acttaatgaa attttagata
    11761 aagaaggtgt tagtgagaaa gacaaagttt ttgttataaa agaaattgat aagttggata
    11821 agctttctga aaaagaaata aaagaaaatt taaaaaaata taatgcagag aaaattttag
    11881 aagtattcaa gaaacctgaa aactattttt ctaagtacga ctcttataag gatgtgaaag
    11941 aatttgtaga ttatttgagt ttatataaag ttgaagcaaa gttttctcct tcacttgcta
    12001 gaggattaag ttactacaac ggaactgttt ttgagataaa gacagataaa atgaaagata
    12061 ctatatgtgg agggggtagt tatgagttta atggaattca gtgcacagga atttcttttg
    12121 gattagatag acttgctatt cttgcagatt ttgactttaa taaaaataat ttcttagttg
    12181 tttctttgga tgaagataga caagcaatta agattgcaga gatgttaaga caaagaggag
    12241 atgctgtttc aatatattat ggtaagcctt caaaggcatt ggaatatgca aattcttgta
    12301 atataaaaca agtcatattt gttggagctc aagaagttaa aagtaaaaag tttaaggtta
    12361 aagacatggt tacaggcaaa gagaaagcaa tcacacttgg aaaaataact aaaaagaatg
    12421 ttattttaaa gaggaaataa aatttaatta aaaaatggga aaatttaaag gagtgggtgt
    12481 aaatccagaa ttacccctag ttttgacaat tacttttctg ctttttttaa taggattttt
    12541 attattgttt tttcaaggaa atcctgattt taatttttat tcttcaaaaa ttgtgattga
    12601 tggacagaat ataaccgaag agctgcattt taaaccagat aaagattatc atactcttta
    12661 tagaaatttt gaaaataagg tttatttttt ccatcatgag aatatttatt caaagaatta
    12721 ctttcttatt aaaagtgtag attgttctca tggaaattat tattttaaag attatgatgg
    12781 tgttccttat cctaaatcct atccctacac tgaaaataat gaatatggtt gtacttttgg
    12841 atatgatact ggttttttaa aaaatcaaga atatacaata ggtgcagaat atgaaattca
    12901 tcctgaaaat ttgtttaaga taggcaataa ttactatatt aaatttgttg catattctcc
    12961 taaaaatcat gtaaaactta caaataaaaa ctttgaggtt tatggagatg ttgtaaaaga
    13021 tgataaatat cttgcagacc aaaatgttat tttatacata ccttattctg gagagattag
    13081 tgggtttaat ataatagaag caaaagattt cgaatttgat tcaaatttca aatataaaat
    13141 aaaatacctt atttttttct ttccaagtat tttctttttt attgtttggt ttatatttgg
    13201 taaagagaat acctatgaat caatacctaa agaattgagt tcttttccaa ataaaagagt
    13261 attttgggaa gttgctactt actttaatcc tcctttttcc aaaatcgata aaaacttttt
    13321 tgctgctaca ctcttaaatt tttataataa aaaaataatt gatataagag aaaaagataa
    13381 agaaatctat attaaattaa acaaatttga aggagatgat ttagaaagaa aaatatataa
    13441 tttacttgga gaagctgaaa gattgcttaa agataaagct aataaaggat tgcttcttgg
    13501 aggtttaact tctaaacaaa aacaaaaatc ttttgatgga gaatatttta atcttaagat
    13561 gtctatgatg gactctttat taagaactga tttttcggaa attagaaatt atgttcaaag
    13621 tgaaggcaag aaatatattc ataacaattc tggaattgtt attcttgttt taactttatc
    13681 attctctttt gcatttattc ttcagtataa attaaatatt tatattgatt ttttggattt
    13741 gatttatgtt ctgttttttg ttgtattttt atttacaatt cctttaaccc gtggaacatt
    13801 attttctaaa tataagggaa attattatgt tgaatatcag aaatggaaag catttaagag
    13861 ataccttaaa aattcttttt caataaaaac tgcaacacat aagacagttg ttatgtggaa
    13921 tgaatattta gtttatgcta ctgctcttgg tgttccaaat cgagtaatta aagagcttca
    13981 agccaacaat ttaattgatg aaaagcaagt taatatgtat aatggagtta ttctcgcttc
    14041 ttcagctagt ttttcaagct cttctggggg tgcaggtgga ggtggttttg gaggagccgg
    14101 aggaggaggt gcaggtggtg gaggtggtgg aggaagataa ccttatttta tctatatatt
    14161 ttataaataa taatatatta ttacaaatat gaataaaaga ggtcaagtta ctatatttat
    14221 tattctagct atattaatag ttgcgagtgt tgttttattt tttgtattta gagataattt
    14281 gggtattgaa gaaactaaag atattcaatc ttctttggtt gttaattttg ttgaagggtg
    14341 tatggataaa acacttaata atagtatata tgctgttgca gctaatggcg gatattcagg
    14401 atattcttat ttttcaagac agtcaactga atctggagtt agatattatt tttttaggga
    14461 gagtaattat atgccttcaa aaagtcttgt tgaaaaacag atagaagaat attttaaaag
    14521 aaaatttttt atttgtataa atgatttttc tgatttcaat gggtattcag tagaagaagg
    14581 gttgttaaaa gtttcagttc gtataaaaga agagaaagtt gttttagaag cagattatcc
    14641 tttaacaata gttcagggta aaaataaagt tcgagtaagg gattttaaat cagagattcc
    14701 ttcaagactt aatataatct attcttctgt taaatatttt atagacaatc ataaaaacag
    14761 aaaaagcctt tgtttaggat gtttgaaaat ggctattgaa aatgatattt acattgatat
    14821 ggaaagttct tatgatggaa cagttgtatt tatatttaga gataattctc cagaattaaa
    14881 ttccaaacct attgaatggg ttttcgcaaa tagatattaa aatggataaa aagaaattac
    14941 ttttatttgt gggtatattt ttatttctta ttttaggatt gagtgtttta agcacagagg
    15001 aagttaataa gaatggtatt aatataatca aagattttgg taatttattt atgaatttac
    15061 ctgaaggaag tataattggt tcagaagatg gtgttaaata tgatagattg aaagaaggag
    15121 gtagatttac ttttactgga gaagagtctt ctttaattat tcaagggagt agttttgaag
    15181 gagttttgcc taaagacagt gacggacatt caagttttat tgaggttaat ttattaggta
    15241 atataactaa agcagatttt gctgttggag agaatggagg agagtatctc attggaggag
    15301 ttaaatttga tttacctcct aattcaagag tttattatga taatgaaaac ggttttagat
    15361 ttccagaagg tacgaagata actagcctca gtgagtttta taatttgggt agagaattac
    15421 aatttgaagg aaataacctt aattttattg atgaagttat tgtaaacgga caattaaaat
    15481 taaacaaata tggatattta attggaaaag gggaagctac ttatgagggc atgaagatag
    15541 ataatttctt tggacatagt gatgttgcaa ttttgaagaa taatttggat aaattaaaag
    15601 aagaagatat ctcagagtta agttggatta aaaaggattc aaatggtctt gaattttcat
    15661 cttctagtac tggttctatt tttgtagagt ttttggaggg aaatgatatt ttgaatgttg
    15721 ggaaacatgg aaagttggaa ttgaggatag atggaggtga taaattaaag ataagtaaat
    15781 tttctggtgt tccaagattt aattatattc cttctgaaaa tggatttaca aatataataa
    15841 atgatggtct tagtttagaa ttagataaag gaggcaaaca ttatgtagat atttctcctg
    15901 ttactctcaa agacttttct ttagataaat atgctgctgt tcctgcaata atagagtcag
    15961 atgttcttga tgaagaaata agaatcaatt ctagaggtac tctttatgga gttttggttg
    16021 ataatacagg cggtaaaaaa gttgaagaaa ttgtttatga tttagagaac ccgattatga
    16081 gtttgaaaac agaagaatta aatggattaa tgggttcttt gaaagagaat attaaagaag
    16141 agggtaattt ggactctgct tttcgtgcat ctcagttttt tttgttgagc gctaaaaata
    16201 atcattttac tacttcggaa aatattgatt tagcaaaaaa gatagtacat tcttcaggag
    16261 aatattctga ttctgtttta tacaaggtta aatttggttt agataaactt tctaaggatg
    16321 atgttaaaat aagctatgaa aagttttcgc agtttactct aaaaaatttg gataatcttg
    16381 attttaaata tggagatggt ttagataagc ttgatgctgt ttattctgtt ttaaataatg
    16441 gattaaaaga aaataaagat tttaacctaa tagaagattt tagtttaaag ataataaaat
    16501 ctgattttaa ttcagataag agtattttac ttcttagtaa gaatataaat gagtataatg
    16561 gagcaaattt tgagagtatg aaacttggag cagaagttgt tggtagatat aatatggttc
    16621 caggagtagt tgattttttg gatttgaaat actctaatat aaaagataaa aaaacatttg
    16681 gaaaagagta tgctcttgct cttaaccaat atttcaaaga taaagattcg attcaaaaga
    16741 gtgactctca gatggcttct tttactttag atcaaatgca tttactggaa tctcagatag
    16801 aacattcaga tattataaga aattctatta tctctaattt agatgctaat tctaaatatt
    16861 atcttattgc taattcagag ggaaataata atctttttac ttctacattt gaaaaaattt
    16921 attctagtat tgataaaaaa gatgtttctg aattgatgaa gtctgttgat cctaatggag
    16981 attattggtt aaaatttaca acagagttgt ctgctagagg caaattacct gagttatata
    17041 aacaagacac tgttttttac agaaagctta ttcaagattc ttttgataat catgatgaag
    17101 atattttatt tgagaatgct gtattaatga ctgaaacatt ccaagatctt tataatcgtc
    17161 cagatatcta tcctggagaa aaagaagctt ttgaagaatt tttaatttct aaatacaagt
    17221 cctctgaatc taataatgaa aaaggagttt atgcttatct cttaaaatta aatacaaatc
    17281 ctaatactca gaaagcagaa aagattatta atgatttgcc tgaacttcgc gatgctatcc
    17341 ttccatcttc tttttctcaa agagatgaaa ttgctgctaa attatacttt tatagcgatg
    17401 aaaactggtt tagtatagca actaatgatt ttaattctaa ttacaaaatg gatttggagt
    17461 ggaacaatgg aagacaagct agattgtcaa aaccgattaa tggcaaaaaa ataataattg
    17521 atttagaact taatgatcct agtgtggatt ttgtaccttc aattgaagaa gatataaaag
    17581 gtcaaactta tctttatatt gctcacagag gacattcttt taatacagag aagacttatc
    17641 cagatttttt atctagtgac acgactaaga taattcatct tgctggttgt ggaagtcatg
    17701 gtgcgcttgc tgactatcag aagaaatttc ctaattcttt ttttatagca gacaaagatg
    17761 ctggagaagg ttttccaagt gataaagtcg cttattttgt tttggaggga atagcagaag
    17821 gtaaattaaa ttgggaggat ttaactcctg attttgcaga agaaaagggc ttagtttttc
    17881 cagaacaaga gagtttacca caatatctta aaagatatga atcagagatg tgaaattatt
    17941 gtttagaaag ttcttttgtt gttcttttta ttattgttat tctgtctcct ttttctatat
    18001 agggaaatat tttgtccata tcttcatttg agggagaaat acatcctaaa gtccagtcat
    18061 atccttttcc accatatcca tgtatttcaa ttaatcctcc tgtttttcct ttggctttat
    18121 cttctttatt tggataattt attaagaaag ctttgtaaaa attggtgctt ccaggtggaa
    18181 gttttttttc tacaatatac attccttcag gagtgcaacc atctccttct gcttgtttgt
    18241 ctgcataagg attaaatcct aaatcaacat tgtattctga atctatttta ccatttttaa
    18301 ctagatataa ttttcttctt tctttattga ctattattga attagagtta tttgcctttg
    18361 attcatttaa agttgaatct acccataaat tgtatttgat taattcttta aaattatctg
    18421 agctatctag ttttatacta tttattgaga ttgtcttgtt tgtatcaata atattttcta
    18481 atgatgagat ggcttgaagt tttttgtttg ttattggtat agttttatga ggagataaag
    18541 ctataataag tccacatatt gctgcagtag caatggatat ttttttaagt ttttccatag
    18601 atttcattaa cctacttatt atataagttt ttctagtgtt atcattttag aataatatct
    18661 agtttattaa tattgtagta taaaattatt tattataata aaaaataaaa aaagataaaa
    18721 taaaaacaaa ataaaaaatt aatttatttt atctggaatg ttgcatatac tgaagcagat
    18781 attttttctt ctccaggttg gatagttgtt gctgcttgtg ttgcttcttt tgctatttct
    18841 tccatgtata ccccatcagt gtttctagat gcgtaaacat tccatggaga atatccaaaa
    18901 tcacttgttt ctactgacaa taattttcca acttttttgt taaaccctga agctattgat
    18961 tctgccttta ttgttgcatc ttctgaagct tcttttagag cttgtgcttt gtatcttttt
    19021 tgagattctg gacttaattc aaagtttatg tagcttattt cagtttttgc ttttacagca
    19081 gcatcaacta cttttgaaac cttattcata ttatcagaag taagttcaac tcttatatag
    19141 tgatttgcaa tatatccttc tgttttagat tttttttctt tgtagtccca ataagtgtgt
    19201 tcataaacac tgaaactttc tgtctttaaa tcatttttat taaatcctgc aattaccatt
    19261 tcttcaacta atttatcata gattttagtg ttttcagatg ttgcttcagc actggtagtt
    19321 ccatttgttt ttatagttat atagactgaa actaaatcag gcattgcttt tattgttgag
    19381 ctaccttgaa cagtcacact gttttttgaa gatggaacaa cagaattaaa tgtactcaaa
    19441 gtaagaagcg caattaccaa tacaactcct gcaattatag atgtgattat tattgcattg
    19501 ttttttccca ttgtttctta tttacctcct ttcaattcta ttaatcatat ttatttcatc
    19561 aaattttttt ccaatttaca attgaaaata ttaatatcac taataatgct cctgctaaaa
    19621 accaaggcca tattaaagga gaagcagatg ctgttaaaga ggtataatca gatgaggcga
    19681 tagttgagca aacaacatct gctccttttg ataatgattc aggtgatagt aattcatgtg
    19741 aatttctttc aacacaatcc ataactggct gttgtataat ttcttgtgtg taatttgtag
    19801 tatattttat tttttcataa gcccatactg caaatgttcc tgctgctgtt aatataaatg
    19861 caggtattat tgatttgaat ttttgagaag ttgatttttt tggagagatt ataatagatt
    19921 tgttagataa ttcgtacatg ataatctttt ttcctttttt gctccagaaa aattctttct
    19981 ttttttgtac aaatcctgat tcaacaagtt tttttatgtt gtaatccaga gtgttcatag
    20041 gtatgtttaa tgcatctgat aaatcttttt gacttgcctc ttttctttca cttaaataag
    20101 cgattatttt tttacaggtc ttgcttccta aaacttcaga gattgctttt gattttgaat
    20161 cttcaaggga tataagcata aagtgatttt cgtccatgta aaatttaaga ataacttatt
    20221 tataaataat ttgaagagtt caaatttgtt tgaagttatc tttttcttct atatctatgt
    20281 ttattttttt gaattcttct tttatttttt gagtatttct tttctcatct tcttttagct
    20341 tttcttttct cttttcaatc caatctaatt catcattcca attctttatg ttttctttta
    20401 gtgtttcaat ttcagaagag actaggttag ttgggtcttc tttaatttct tttttttgtt
    20461 tttccaatat ttcttctttc atagatatat tttttatttg ttcagatatt ttgtcattat
    20521 tcaatcctgc ttcatttaag agatttacca gtgattttcc tttatcttct ttaaattcca
    20581 ataaaaaact ctctttgtat ctttttatgt aattcatttt ttcaggaaat atatgaaaga
    20641 aacttgagag agatttaaag tctatatctt gtctaagctg agatatttgg tttttaattt
    20701 cttcttcttt tataataata gattctttta gttgaatatt ttttatgtat tcttcactat
    20761 tttttatctt gtttaatatt gttaattttt cattaaggat attttctttt tcttttatct
    20821 tagtttttag ttcattggat tcctcatcta tcttatttaa tttatcctgt atttctagat
    20881 tttcatttaa ttttgattct gagaatagaa tgcttttctt ttcatttatt atattcttat
    20941 tttcattgta tatctctaat atttcttttg aaaatttctt taaactttct tttatagcgc
    21001 ccatctcttt tcctattaat actgtggcac gttcataact tttcattgaa ctttcattga
    21061 attttgaaaa tgaataattt atgttttcta ttatttcttg aaaattatct atgtttttgt
    21121 gttcttctaa attatccatt aatctttcta taaattcaat atattttttt cttccttcat
    21181 atacaattgc cttgatttta ttgttttttt cttttgattc tatgtctact ttttctacaa
    21241 tttcaacttt ttcttgaata tcttttgaga aagaatctat gtttgttttt attaaagata
    21301 tgattgaatc ttctctttct tgtatttttt ccttattttt atctagaatt ggtttaattt
    21361 cagataagtt tacctttatt cttttaggtt ctttttcttc tgcttcttta aagatattct
    21421 ttaaaagatt taatattccc ataatatttc atatatattc tgcttaaaaa acctttttta
    21481 atacattaca gcatagaaat gttaccttat tttgtatttg gtcaaataat tatttctctt
    21541 ctccaggaat tttcaccttc tttgaagtct ttaaatgact ttatcttaag aggggtttta
    21601 aatagaggac aatctaaaac aaaagtctca aattctcctc cttctcctgc aggatgtatt
    21661 ttgtattttt gatttagtat tttcgcttct tcaatgaatt tttcatctat atttctacct
    21721 aaccaagatt gatttaaagg atatgcaaaa actcctgtta ttataacttt gaaattttct
    21781 cttaaaagtt ctttaagata aggtatttct tctttatgcc aaagaggatt tatgcatttt
    21841 atatttaatt catcacatat tttttgtatt cttgaatttt gatattctga atataaaacg
    21901 cctgttacaa ctccttgaat tttgtatttt tttattgctt ttattattgc tttttttaaa
    21961 tcttttaatt cttcttcttt ttttcctttg gttttttgta ttattaatgg aatgtccata
    22021 gattttgctt ggtgtttggt tttttttatg cttggtgtgt gaaacatata agattctttg
    22081 ttttctgaac agagagttat cagacagctt atttgatttc cttggttttt tgcaagataa
    22141 attgaataaa cagaatcttt tccacctgaa aataaagaag ctaatttcat atttttatta
    22201 atttgatgtt atttttaagt ttatttgaaa actgcaattc cctggagaac tatttctata
    22261 agtcccccgt tttcttgatc atagtttgtt ctttgttgta ttccaacaat ttttcttaat
    22321 ttaagtcttg gtttttttag t
//
LOCUS       DAOPCH010000008        19381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_2931_length_19381_cov_4.720687, whole genome shotgun sequence.
ACCESSION   DAOPCH010000008 DAOPCH010000000
VERSION     DAOPCH010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 19381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19381
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2931_length_19381_cov_4.720687"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..599)
                     /locus_tag="PLU55_01050"
     CDS             complement(45..599)
                     /locus_tag="PLU55_01050"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPJ86679.1"
                     /translation="MGVERLIKARRSIRKFKSSKPDWREIIECIDSTRYAPMAGNNFS
                     LKFILVEDGEKIQKLAEAAQQNFITKAKYVIVVCTTPGRTETAYGERGSKYLKQQAGA
                     AIQNLLLKLTECKLATCWVGHFVDEQVKDILDVPDEIDVEAMFPIGYPYEKPKEKRYI
                     ELDSILYFNRYDNKRMEEESKLSA"
     gene            complement(645..1766)
                     /gene="mnmA"
                     /locus_tag="PLU55_01055"
     CDS             complement(645..1766)
                     /gene="mnmA"
                     /locus_tag="PLU55_01055"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPJ86680.1"
                     /translation="MKEKVLVAMSGGVDSSVAALLLKKQGYEVIGAFMKNWSDTKDPL
                     TGRCAWRSERKMALKIASKLNIPLITLDFEKQYKKEVINKMFDKYKKGITPNPDIDCN
                     QKIKFPLLLKEAQKRKINLIATGHYARVKKSKNKDKKDMYELLRGKDETKDQSYFLYK
                     ITQEELSHCLFPVGEYTKEKVREIAKENNFPNYNKKGTVGICFVGKVNLKKFLQKKIK
                     PKKGKILNPDGKIIGEHDGIYYYTIGQRLGPRYGFEIDRGNKNKPSKWYVAKKDVKTN
                     TITAAPENHPLNFRKNIIIKNLHLISNKKETFQNKPTKVFSRIRHVGELLPSIFHYNK
                     KTKQFQITLNEAITGVSEGQAVVIYEKDKVIGGGEIIFQ"
     gene            complement(1801..1874)
                     /locus_tag="PLU55_01060"
     tRNA            complement(1801..1874)
                     /locus_tag="PLU55_01060"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1838..1840),aa:Asn,seq:gtt)
     gene            complement(1913..2455)
                     /locus_tag="PLU55_01065"
     CDS             complement(1913..2455)
                     /locus_tag="PLU55_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPJ86681.1"
                     /translation="MKKIISEKVIRIIKNKANLEKELDVKLNIIGKEITIIGEPEEEY
                     IAEKVIEAIDFGFPISSALSIKKEEFLFEILNIKEYTNQKDFERVRGRIIGKDGKALK
                     TISTLSNCHIELSGNKLGIIGEAECIRNAEEACKLLIKGTKHSNVYSYLEKHGHSEPL
                     LDLGLKEAKSTGKNKKINKQ"
     gene            complement(2484..3719)
                     /locus_tag="PLU55_01070"
     CDS             complement(2484..3719)
                     /locus_tag="PLU55_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86682.1"
                     /translation="MKRGDKKGLSTVIATLLIILLVLVAAGIIWVVVKKVISKNSELV
                     AFESTTVDLEIISVTQPKNPATGNIISTKVKVRRNPGEGEIVGLIFSITNKNGESYVF
                     EKNESIKQLEVKLFELDYNGSIVSISVAPIFLTESGKKVIGNIGDTHYEPDYRGAGSG
                     TGEDDDCTPDCIRGAAGYVLECGPSENNCGSCGTCSSPLLCDYGICCEIGHHNYQGTC
                     QPDCDPSDCGTRQCGPAPAKPGCGENFCGTCNEQNGEVCNENTWTCDVCVPTCGPTQN
                     CGLDPTCHSSCGECNEQNGEVCNTSNMCEVFVEPIENTGYVNSYWPRPDGRMLFDSDD
                     LPKDKTVIDFYRHYVRFPENQNMKNECLMIYDFVTPINPNTYNMSYVKISENPTKIED
                     GDKYEIYQTIQGCCQGGYC"
     gene            complement(3784..4155)
                     /locus_tag="PLU55_01075"
     CDS             complement(3784..4155)
                     /locus_tag="PLU55_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HPJ86683.1"
                     /translation="MTDKFKENNQEFSEEENEVNENENSEEIKIARAKIPRGEEVIGI
                     IEERLGGNKMKVKCLDGKTRNSRVPGRLKRKLWLRPGDTVLVEPWELDKSKGDVIFKY
                     PSNQIEWLKRNGYLKTEEKEF"
     gene            4257..4493
                     /locus_tag="PLU55_01080"
     CDS             4257..4493
                     /locus_tag="PLU55_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86684.1"
                     /translation="MSADYNKNDLEFRAKYLLKTKIKGKDKINLDILKGNIHEINYLV
                     REGYLVEKGDTVGSFHYELTNKGNCFISICKKFK"
     gene            complement(4530..4826)
                     /locus_tag="PLU55_01085"
     CDS             complement(4530..4826)
                     /locus_tag="PLU55_01085"
                     /inference="COORDINATES: protein motif:HMM:NF016157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 family protein"
                     /protein_id="HPJ86685.1"
                     /translation="MFVNVIKSYRDVVAICDKELIGKKFEEGNFQLDVKENFFKGREA
                     STEEVSKIMKEMKLEDSTFNIVGEKSIKIALETGIITKQGIGKIDGIPFALVLI"
     gene            complement(4829..5023)
                     /locus_tag="PLU55_01090"
     CDS             complement(4829..5023)
                     /locus_tag="PLU55_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HPJ86686.1"
                     /translation="MVEAYCVKCKKKGQKMKEAVMTRTAKGGFMAKGKCPDCGTIMCA
                     MMSAEKAEKAIKDGKAKKGY"
     gene            complement(5112..5522)
                     /locus_tag="PLU55_01095"
     CDS             complement(5112..5522)
                     /locus_tag="PLU55_01095"
                     /inference="COORDINATES: protein motif:HMM:NF003067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HPJ86687.1"
                     /translation="MTDYEKLLDEAYKKVKVVEGKSDRFEVPVIEGHIEGKKTILTNF
                     LIIASYIRRNPEHFQKFILKELATSGQREGERLILNNKIASSKINAKIDRYVKEFVIC
                     KECGKPDTELKKENRLSFMQCLACGAKHPVREKI"
     gene            complement(5567..6202)
                     /locus_tag="PLU55_01100"
     CDS             complement(5567..6202)
                     /locus_tag="PLU55_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPJ86688.1"
                     /translation="MAEEGQFKRNIAYKLKIGDILLGKPIIDNERFSFLELGDKRIVR
                     VNIIGNIVDKYESDGERKYIFFKLDDGSGQISLKVFGDDIEKFKEINQGLTVLVIGTL
                     RHWNNETYIQPEIIKEQNTKYLLVRKLELEKGGDSMKKAPPMERSKIVAIKDSIINLI
                     KEAEENGGIEKEQIILKLKETSPDIINQEIKKFLEEGIIFEPRPGKVRYLG"
     gene            complement(6251..8329)
                     /locus_tag="PLU55_01105"
     CDS             complement(6251..8329)
                     /locus_tag="PLU55_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minichromosome maintenance protein MCM"
                     /protein_id="HPJ86689.1"
                     /translation="MGDGTNYSEEDYSAASNPEEGQKKRPKSEELIIEAKNFFNVNKE
                     DLGESIRKKTNVVLVDFMKLTEFSTRLSDEIIVNPEETLSIIELAVEELGLVKNARVR
                     LKNLSETHQIKIRNIRSKHLNELVILEGIIRQASDVRPQVVNAKFECPSCGTIISVLQ
                     IEKKFREPTRCSCGRRGGFRLISKDMVDTQRLVIEESPESLSGGEQPKRMNVFVKEDL
                     VEPKMEDKTTPGAKVKVIGILKEVPVPLSTGGLSTRFELAVEVNNIIPMEETFEELDI
                     TDEDERQIHELSEDPKIFENLAKSIAPSVWGYEEIKKALILQLFGGVQKQMSDGSKSR
                     GDIHILLIGDPGVAKSVTLVFMSNISPKGRYVVGKSASGAGLTATVVRDEYLKGWSLE
                     AGAMVLANKGLVCIDELEKMDPQDRSAMHEAMEQQTVTISKANVQASLKAQTSVLAAA
                     NPKFGRFDPFMPIPQQIDLPPTLINRFDIIFTLRDLPDKNKDEKIAAHVLREHQKETK
                     EVLIDSNLLRKYVAYAKQKVNPVLSTEAVEELKKFYVELRNKPVMSESAMRPIPISAR
                     QLQALVRMSEASARLRLSEEVSKEDAKEAIELMKYYLMQVGYDYESKTFDIDRISGKF
                     SSSQRNRIFMVKEVITALESKMGKMIPYEEIEKELQGKMEKSELEDIIGRLVKDNEIY
                     RPKKGYIGVF"
     gene            complement(8387..8944)
                     /locus_tag="PLU55_01110"
     CDS             complement(8387..8944)
                     /locus_tag="PLU55_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HPJ86690.1"
                     /translation="MAQKQSNYKIQNIVATASLGKPVPLTKLARTQPNTEYNPETFPG
                     LVLRIKEPKSAVLVFSSGNLVCTGTKSLTQVREVIDAVIKQLKKISVNITGKPKITVQ
                     NIVASGSIELKLNLNFLALEMENTEYEPEQFPGLVYKLIEPTATFLLFSNGKLVCTGT
                     KNRQQLEDSMDQLLKNVKAALKNYK"
     gene            complement(9013..10920)
                     /locus_tag="PLU55_01115"
     CDS             complement(9013..10920)
                     /locus_tag="PLU55_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86691.1"
                     /translation="MKKRGSKKPKKEDEIPPILICLILLALILFILIFKNLVLTTNVI
                     QNHLTPKVIENPPTPENCSNESIKKVWEFIFKGSSENLTITSAPYNNTNSSEEESEIW
                     SLLLNEKCPAYKVYQINGTNIRTLIGIDVWFLMNMKMAMAIDGNYPPEEMENIIQRIS
                     SSEQNEMENAPEYLLLNFLANRTIKRNINTSQEAKTEFESVFNISSSNWTKEEFKVDG
                     ITENLTAFSFFENGSLENITLFGESIDPMGIIAKAGMVLQNYSMNIYLYTDISLLAFT
                     KELERLYGNWTSPINTSLKNIEVKINDSKLKMSEEIGLFSESRSGIQRIEINQDEQTI
                     IETEINFTEEGSLDWTKIILKKQETDSTRGYIIINGLNYTKNVTVDRLNDKSESVCIK
                     DKDIYSIDEISPECNAIDEYILECPGNLTSTARNLTCNISGNKFIITGLEHSAILEII
                     PPEIPCVQNWQCDEWSETSKSCGYRTCTDLNNCGNITGKPLEYQECPVCIPNWKCTQF
                     LPEKCPREEKRTRTCTDINDCGTEKGKPELTQTCERKNILVYILITLLGIAVIILIVI
                     ILKRIIIRREKLKERPRTEQGQYKNDPNYPSQIEQYYGSDQTHNFKEQPETNPQSENY
                     QEKFPDKYWPK"
     gene            complement(10963..12666)
                     /locus_tag="PLU55_01120"
     CDS             complement(10963..12666)
                     /locus_tag="PLU55_01120"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HPJ86692.1"
                     /translation="MLYSKLAELYENLGNTTKRLEKIEILSKFLEYLSEKDKDILYLL
                     NGEIYPTYDERKIGISNQLVIKAISKASGNSPDKVVQEWKKIGDLGKVAEKLINTKKQ
                     STLFLKSHLTTDKVIENLRKLPELEGEGTIEKKLILITELLVSASPIEALYIVRTLLG
                     DLRIGIQESTIRESLAKAFFNSDKESSKIIQEAIDHSNDLAKVFLISKKGKISEFDKI
                     GLEVGKPIKAMLAQKVKNIEEGFKAVGKPCAIEYKYDGFRLIIHKKDSKIKLFTRKLE
                     NVTKQFPEAVEYIQKYVRGESFILDSEAIGYNKKTKEYTPFQAISQRIRRKYNIEKIR
                     EELPIEINVFDILYYNGKSLIKEPFEKRTQLIRKIIKETPYKIVSSKQIITDDLNKAE
                     EFYKKALKDNQEGIMMKNLSTAYQPGNRVGHMLKVKPSEKDLDLVITGAEYGTGKRSG
                     WLSSFILSCMGKEEQEFLEIGRVGTGIKEKDEQEGVSFNELNDLLKPLIIKEQGKDVK
                     IKPKIVVAVTYQEIQESPNYSSGWALRFPRITSLRPDKHLSEIASLQEIEKEVKGQKS
                     N"
     gene            complement(12691..13068)
                     /locus_tag="PLU55_01125"
     CDS             complement(12691..13068)
                     /locus_tag="PLU55_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HPJ86693.1"
                     /translation="MVEKKEIYKCEICGNIVEVVHEGRGVLICCGQPMKLQKENTVDA
                     SQEKHVPIIRGKKVKVGSIAHPMVTEHYIEWIEATSETGETAKVFLKPDSKPEAEFSF
                     KPVSAREYCNLHGLWKVKINKNN"
     gene            complement(13077..13658)
                     /locus_tag="PLU55_01130"
     CDS             complement(13077..13658)
                     /locus_tag="PLU55_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPJ86694.1"
                     /translation="MELKGSETEKNLLKAFAGESQARMRYNYFASVAKKEGYEQISAI
                     FTETADNEKEHAKVFFKYLEKSQGLPVEITASYPAGKIGTTKENLLAAANGEQEEWEE
                     LYPEFEKVALKEGFKDIAESFHEIAEVEEQHEKRYRKLLKNIEEKRVFQKEEIVKWKC
                     RNCGYIHEGKEAPDECPACKHPQSYYEILEENY"
     gene            complement(13706..14296)
                     /locus_tag="PLU55_01135"
     CDS             complement(13706..14296)
                     /locus_tag="PLU55_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15e"
                     /protein_id="HPJ86695.1"
                     /translation="MYYYIKKAWKKPDSKVLMARMKEWRESPTQIKVEKPLRLDRARA
                     LGYKDKKGFVVIRVKVKRGGHKRPRPIKGRRGKRMHTRKNLKMSYKWIAEQRVANKHT
                     NLEVLNSYKIGKDGINYFYEVICVDPQRPEIKNDKTINWIVNRKNKNRVFRGLTSSAK
                     KSRGLRDKSPTNKNRPSRRAGQKPNPPSGRRYILHR"
     gene            14480..15010
                     /gene="dut"
                     /locus_tag="PLU55_01140"
     CDS             14480..15010
                     /gene="dut"
                     /locus_tag="PLU55_01140"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPJ86696.1"
                     /translation="MKKSVAKKAVKNKKKKKARHEVTLNFLKISKSAEAPEYALDTDA
                     GFDLKALESVTIFPFDQKKVSTGIAIEVPEGYVGIVRDRAGIVQKMNVHTVAGTFDSG
                     FRGEVSIMLVNMNDKTIEIEKGMRIAQIILVPIVKAKITEVKKLSSTERGDKGFGSTG
                     MKEIIRELNKISEKSI"
     assembly_gap    15123..15222
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15313..15387
                     /locus_tag="PLU55_01145"
     tRNA            15313..15387
                     /locus_tag="PLU55_01145"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15348..15350,aa:Ile,seq:gat)
     gene            complement(15395..16075)
                     /locus_tag="PLU55_01150"
     CDS             complement(15395..16075)
                     /locus_tag="PLU55_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPJ86697.1"
                     /translation="MFSKKTCQRCGKKTGKGNNFCPSCGFPLGKKGKKEEFGMLGEND
                     SFNETDMISNSLFGGMMGNVMNKMISNTIRMLEKEMEKEMRMSKQSPTNNNFPKTKIK
                     LMINGKEVDLNNGMQKQGENKKEKQESIPLRFKKFSKEQIKKFSKLNKKEPKTELRRI
                     ADKIIYEIEMPGVESIENVTITPLESSIELKAIAEEKAYSKSIPLNLPIIGYEFSKGL
                     LVLEFKGN"
     gene            16168..16560
                     /locus_tag="PLU55_01155"
     CDS             16168..16560
                     /locus_tag="PLU55_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86698.1"
                     /translation="MAIKAIKEIPLKSRHVRWNFEKSEDNFYMGNPLTRYYFAIIDNR
                     EANRKELSNKIANQLMKSNYWVIPVEQGFNLKVKNSEWGLSFVVYDALASYENLNFSK
                     LNVVSKRDYKLCKKEVLSIEDYLTRIYE"
     gene            16636..17430
                     /locus_tag="PLU55_01160"
     CDS             16636..17430
                     /locus_tag="PLU55_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86699.1"
                     /translation="MKKEKRLKKFNISNRLAYTLMVILSIALLGIGVWAAAPNPGHSA
                     NELELDWGSKSFCEGSVNPCDSLTPSQCSSQSTCYMSCTGSGGYVSCSAYTDESICRD
                     HGCMWEGSSRYCYGSGGETVSCSSFYSQSDCDRYTMVGCYWGCSGNAHSCSTFTNPTY
                     CNRQSGCKWVTRQRSITSNDRGINISNLQVNSLCDISGSNCVQVRGKEASKEGNYNTG
                     DDSTRRMRINQYASCPTGYYVSAVRSVSYSASTSALRVTCTPLPIR"
     gene            17519..17908
                     /gene="metG"
                     /locus_tag="PLU55_01165"
     CDS             17519..17908
                     /gene="metG"
                     /locus_tag="PLU55_01165"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HPJ86700.1"
                     /translation="MGDRDNKESKESKENNIEGIVEFNEWQVIDLRVAQIKKVEDISG
                     ADKLYKLTLDVGEEIGERVICAGIKQYYSKEELEDKKIVVFVNLKPRLMRGIESQGML
                     LAAVSEDHSEVVLVCPEKSIKNGCKIS"
     gene            complement(17947..18747)
                     /gene="minD"
                     /locus_tag="PLU55_01170"
     CDS             complement(17947..18747)
                     /gene="minD"
                     /locus_tag="PLU55_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATPase MinD"
                     /protein_id="HPJ86701.1"
                     /translation="MNKIIVVTSGKGGVGKTTTAINLGAAINYFGKDVLIIDGNLSTP
                     NVGLHLNSPEVPINLNHVLRGKAEPKEAIYEHVSGLKVMPSSLSIKELKKIKPEKISD
                     FKKEFQTLSEYIIVDSSAGLGDESIAAINLADEIIVITNAEMPAITDALKTIKLAEQM
                     KKTILGVIVTRVRKDSIEMSPESVKDMLEAPILGMIPEDITVKKALNKKDAVVHTHPH
                     SNASRAYKEIAARITSTEYNSKKDRYPFWRRILKRENPYFWKMTMKKG"
     gene            complement(18775..19218)
                     /locus_tag="PLU55_01175"
     CDS             complement(18775..19218)
                     /locus_tag="PLU55_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86702.1"
                     /translation="MEEIIQEKISADHLLYVSLKYTKTCDVIKNLILRWRKMIETSIE
                     EILKSAKKKKKISSIPSNPLGKIEEIKKLFKKDSNFLEVIELYEMFRKIDELRTERIG
                     EFRKNVALRIFYQGKEINVDLEKLKEYAEKLEKFISTTKQFLLSK"
BASE COUNT         5336 a         3030 c         2713 g         8202 t
ORIGIN      
        1 tataatttta gaaatgtttt tatggaggat gtagattaag cagattaagc gcttaactta
       61 gattcttctt ccattctttt gttatcatat ctgttaaagt ataaaatgct gtctaattca
      121 atatatctct tttcttttgg tttttcatat ggataaccta ttgggaacat tgcttcaaca
      181 tctatttcat ctggaacatc caatatatct ttaacttgtt catctacaaa atgccctacc
      241 caacatgttg ctaatttgca ttctgttaat tttaaaagta aattttgtat tgctgcacct
      301 gcttgctgtt taagatattt gcttcctctt tcaccataag ctgtttcagt tcttccagga
      361 gttgtacaaa caactataac atattttgct ttagtgatga aattttgttg tgctgcttct
      421 gcgagttttt gtattttttc tccatcttca actaaaataa atttcaaaga aaaattgttt
      481 cctgccattg gagcatatct tgtgctatca atacattcaa ttatctctct ccagtcaggt
      541 ttagaagatt tgaatttcct tatacttctt cttgccttga ttaatctttc aacacccata
      601 tttaaattaa gagagtgtag tttaaaaatt attcttattt ttatttattg gaagattatc
      661 tctcctccac caataacttt gtctttttca tatattacta ctgcttgtcc ctcacttact
      721 cctgttattg cttcatttaa ggttatttga aattgctttg tttttttgtt ataatgaaag
      781 atactaggaa gtaattcccc tacatgacgt attcttgaga aaacttttgt tggcttattt
      841 tgaaatgttt cttttttatt tgaaattaaa tgtaaatttt ttattattat gttttttcta
      901 aaatttaagg gatgattttc aggagctgca gtgattgtat ttgtttttac atcttttttt
      961 gcaacatacc atttggaggg tttattttta tttcccctgt ctatttcaaa tccatatctt
     1021 gggcctaatc tctgtcctat agtataataa taaattccat catgttctcc aataatcttt
     1081 ccatcagggt taagaatctt tccttttttt ggttttattt tcttttgcag gaattttttt
     1141 aggtttactt ttcctacaaa acatattcca acagttcctt ttttgttata atttggaaaa
     1201 ttgttttctt ttgcaatttc tcttactttt tctttagtat attctccaac tggaaataaa
     1261 cagtgtgaga gttcttcttg ggttattttg taaagaaaat aagattggtc ttttgtttcg
     1321 tcttttcctc ttagaagttc atacatatct tttttatctt tgtttttact ttttttaact
     1381 ctagcataat gtcctgttgc aattaagttt atttttcttt tttgagcttc ttttaaaagg
     1441 agagggaatt ttattttttg attgcaatca atatctggat taggagtaat accttttttg
     1501 tatttatcaa acattttatt gataacttct tttttgtatt gcttttcaaa gtctaatgtg
     1561 ataagaggaa tatttaattt agatgctatt tttaatgcca tttttctttc acttctccaa
     1621 gcacatcttc ctgttaaagg gtcttttgtg tcagaccaat ttttcatgaa ggcccctatt
     1681 acttcatatc cttgtttttt aagaagaaga gctgcaacac ttgaatctac tccacctgac
     1741 attgctacta aaactttttc tttcataaaa taatgagtaa ttgtatattt aaaaataaag
     1801 cgacccgggc aggactcgaa cctgcgactt cacggttaac agccgtgcgc tctaaccaac
     1861 tgagctacca ggtcattttt ataaaagatt tagcttagaa ccttatttaa aattattgtt
     1921 tgttgatttt tttatttttt cctgtgcttt ttgcttcttt taatcctaaa tctaggaggg
     1981 gttctgaatg tccgtgtttt tctaaataag aatatacatt agagtgtttt gttcctttaa
     2041 ttaagagttt acaagcttct tctgcatttc ttatacattc tgcttctcct attattccta
     2101 atttatttcc agaaagttca atatgacagt tgcttagggt tgaaatggtt tttaatgctt
     2161 ttccatcttt tcctattatc cttcctctta ctctttcaaa atctttttga ttagtgtatt
     2221 cttttatatt taaaatttca aataaaaatt cttctttttt aattgaaagt gcacttgata
     2281 ttgggaaacc aaagtcaata gcttctatta ctttttctgc aatgtattct tcttcaggtt
     2341 caccaattat tgttatttct tttccaatta tatttagttt tacatctaat tctttttcta
     2401 aatttgcctt attttttatt atccttatta ccttttcaga gattatcttt ttcatgtttt
     2461 attaagagag ttttttatag tatttaacaa taaccccctt gacagcatcc ttgtatagtt
     2521 tggtatattt catatttgtc cccatcttct attttagtag ggttttcaga aattttaaca
     2581 taactcatgt tatatgtatt aggatttatt ggtgttacaa agtcatatat cattaaacat
     2641 tcgtttttca tattttgatt ttctgggaat cttacataat gtctataaaa gtcaattact
     2701 gttttgtcct ttggtaaatc atcactatca aaaagcatac gtccatctgg tcttggccaa
     2761 taagaattaa cataccctgt attttcaata ggttcaacaa atacctcaca catattagat
     2821 gtattacata cttctccatt ttgttcatta cattctccac agcttgagtg acatgtaggg
     2881 tctaatccac aattttgtgt tggtccacat gtaggaacac aaacatcaca agtccaagtg
     2941 ttttcattac atacttctcc gttttgttca ttacatgttc cacagaaatt ttctccacat
     3001 cctggttttg ctggagcagg accacattgt cttgttccac aatcagatgg gtcacaatct
     3061 ggttggcatg ttccttggta attatgatgt cctatctcac aacaaattcc ataatcacat
     3121 aaaagaggag aagaacatgt accacaactt ccacagttat tttctgaggg cccacattct
     3181 aatacatatc ctgctgctcc acgaatacaa tctggagtac aatcatcatc ctctcctgtc
     3241 ccacttcctg ctcctctata atctggttca taatgggtgt ctcctatgtt ccctattact
     3301 ttttttcctg attctgttaa gaatataggt gctacagata tactaactat tgaaccatta
     3361 taatcaagtt caaataattt tacttctaac tgttttatac tttcattttt ttcaaaaaca
     3421 taagattctc cattcttatt tgttatagaa aatattaatc ctactatttc tccttctcct
     3481 ggatttctcc taacttttac ttttgttgaa attatatttc ctgtagcagg gttttttggc
     3541 tgtgtcacag aaattatttc taaatctact gtagttgatt caaaagcaac caactctgaa
     3601 tttttactta ttaccttttt tacaactacc cagattattc ctgctgcaac aagaactaga
     3661 agaattatta aaagagttgc aataactgta gagagtcctt ttttatctcc tcttttcata
     3721 attttaaata gaaatttagg ttaataaata ttgagtttgg tatattttag taatattgga
     3781 accttagaat tctttttctt ctgttttcag atatccattt cttttaagcc attcaatttg
     3841 atttgaggga tatttgaaga ttacgtctcc tttagattta tctaattccc agggctcaac
     3901 aaggactgta tctcctggtc taagccatag ttttcttttt aatcttccag gaacacgaga
     3961 atttcttgtt tttccatcaa gacatttaac cttcatttta tttcctccaa gtctttcttc
     4021 aataatacct ataacttctt ctcctcttgg tatttttgct cttgctatct ttatttcttc
     4081 agaattttca ttctcattaa cttcattttc ttcttctgag aattcttggt tattttcttt
     4141 aaatttgtct gtcatatttt agataaaaga ttatgtttta taaatatata gtttaaatta
     4201 agagaatatt tataatctat ttgttgtttt taataaaact tattggagat gtttgtatga
     4261 gtgcggatta taataaaaac gatttggaat ttagagctaa atatttattg aagacaaaaa
     4321 ttaagggaaa ggataagata aatttagata ttttgaaagg taatattcat gaaatcaatt
     4381 atcttgtaag agaaggatat cttgtagaaa aaggagatac tgtgggttct tttcattatg
     4441 aacttacaaa taaaggtaat tgctttattt ctatatgtaa aaaatttaaa taaattttaa
     4501 attaaagatt tgtattgctg ttaactatat tatataagta ctaatgcaaa aggtatccca
     4561 tctatttttc ctataccttg ttttgttatt atacctgttt ctagtgctat ttttattgat
     4621 ttttctccca caatattaaa agtggaatct tcgagtttca tttctttcat tattttagag
     4681 acttcttctg tggaagcttc tcttccttta aagaaatttt ctttaacatc taattgaaaa
     4741 ttaccctctt caaatttttt tcctattaat tctttatcgc aaatagctac tacatccctg
     4801 taggatttta ttacgtttac aaacattttt agtaaccttt ttttgcttta ccatctttta
     4861 tggctttttc tgctttttct gcagacatca tagcacacat aatagttcca caatcaggac
     4921 attttccttt tgccataaat ccacctttag cagttcgagt cattacagct tctttcattt
     4981 tttgtccttt ctttttacac tttacacaat aagcttcaac cattttttac ctcccttttt
     5041 aatttaattg ggacagggtt ttcaagaaat tttgttttat aaatatttag attttatttt
     5101 tttaatattt tttatatttt ttctctaaca ggatgctttg caccacatgc taaacattgc
     5161 ataaaactta atctattttc tttttttaat tctgtatctg gttttccaca ctccttacag
     5221 attacaaatt ccttaacata tctatctatt tttgcattta ttttagaact tgctatttta
     5281 ttgttaagaa ttaatctttc tccttctctc tgtcctgatg ttgcaagttc tttaagaata
     5341 aatttttgaa aatgttctgg atttcttctt atgtatgatg caattattaa gaaatttgtc
     5401 agtatagttt ttttcccttc tatatgtcct tctattactg gtacttcaaa tctatctgat
     5461 ttgccttcta caactttaac ttttttatat gcttcatcaa gaagtttttc atagtctgtc
     5521 attaatttct aaaaaggaat gtctttataa ggattttgat gagtaattaa cctaaatatc
     5581 ttacttttcc agggcggggt tcaaagatta ttccttcttc aaggaatttt tttatttctt
     5641 ggtttattat atctggagag gtttctttta attttagaat tatttgctct ttctcaattc
     5701 ccccattttc ttcagcttct ttgattaaat ttattatact atcttttatt gcaacaatct
     5761 tgcttctttc cattgggggt gcttttttca tagaatctcc tcctttttca agctctaatt
     5821 ttcttacaag aaggtatttt gtgttttgtt cttttattat ttcaggttga atgtatgttt
     5881 cattgttcca gtgccttaat gtaccaataa ctaaaacagt taatccttga tttatttctt
     5941 tgaatttttc tatatcatct ccaaatactt taagactaat ttgtcctgaa ccatcatcaa
     6001 gcttaaagaa gatatatttt ctttccccat cactttcgta tttatctact atatttccta
     6061 ttatatttac acgcactatt cttttatctc ctaattctaa aaaagaaaat ctttcattat
     6121 ctataattgg ttttccaaga aggatatccc ctatttttag tttatatgca atgtttcttt
     6181 taaattgtcc ttcttctgcc atctttttat gctctctaaa aatttttgag tgtttataag
     6241 tttttatggg ttagaaaacc ccaatatatc cttttttagg tctgtaaatt tcattgtctt
     6301 ttacaagcct acctattata tcttccaatt cacttttttc catttttcct tgaagctctt
     6361 tttcaatttc ttcataagga atcattttac ccattttact ttcaagagca gttattactt
     6421 cttttaccat aaatattctg tttctttgtg atgaagaaaa ttttcctgaa attctatcta
     6481 tgtcaaaagt ttttgattca tagtcatatc ctacttgcat taagtaatat ttcattaatt
     6541 ctattgcttc ttttgcatcc tcttttgaaa cttcttcact taaccttaat cttgccgatg
     6601 cttcagacat tcttactaat gcttgcaatt gtcttgccga gattggaatc ggacgcatcg
     6661 cgctttcaga cattactggc ttgtttctta gttcaacata aaattttttt agttcttcaa
     6721 ctgcttctgt gcttaatact ggatttactt tttgttttgc atatgctaca tattttctca
     6781 ataaatttga atcaattaaa acttcttttg tttctttttg atgttctctt aatacatgtg
     6841 ctgcaatttt ttcgtctttg tttttgtcag gtaaatctct tagtgtgaaa attatatcaa
     6901 acctatttat aagggttgga ggtaaatcta tttgttgagg aataggcatg aatgggtcaa
     6961 atcttccaaa ttttggattt gcagcagcaa gaacagatgt ttgtgctttc aatgaagctt
     7021 gaacatttgc ctttgaaata gtcactgttt gttgttccat agcctcgtgc atggcacttc
     7081 tatcttgagg gtccattttt tctaattcat caatacaaac caatccttta tttgctaaaa
     7141 ccattgctcc tgcttctaga ctccatcctt tcagatattc atctcttaca acagttgctg
     7201 taagtcctgc tcctgaagca gatttaccaa caacatatct tccttttgga gagatatttg
     7261 acatgaaaac tagagttaca ctttttgcta cacctgggtc tcctattaaa agaatatgaa
     7321 tatctcctct tgattttgaa ccatcagaca tttgtttttg aactcctcca aataactgaa
     7381 gaattaatgc tttctttatt tcttcataac cccaaacact tggagcaata ctctttgcta
     7441 aattttcaaa tattttagga tcttcagata attcgtgaat ttgcctttca tcttcatcag
     7501 ttatgtctaa ttcttcaaag gtttcttcca taggaataat gttgtttact tcaactgcta
     7561 attcaaacct tgttgataat cctcctgttg agagaggaac aggaacttct tttaaaattc
     7621 caataacttt tacttttgct cctggagttg ttttatcttc catttttggt tcaactaaat
     7681 cttcttttac aaacacattc attctttttg gttgttctcc tccagaaaga gattcaggag
     7741 attcttcaat tactaatctt tgagtatcaa ccatatcttt ggagattaat ctgaatcctc
     7801 ctcttcttcc acatgaacat cttgtaggtt ctctgaattt tttttctatt tgtaatacag
     7861 agataattgt tccacaactt ggacattcaa atttagcatt tacaacttga ggacgcacat
     7921 ctgaagcttg tctgattatt ccttcaagaa ttactaattc atttaagtgt tttgaacgta
     7981 tgtttcttat ttttatttga tgtgtttcag agaggttttt taaacggact ctcgcatttt
     8041 taactaatcc tagttcttct acagctaatt ctattatgct taaagtttct tcaggattaa
     8101 ctattatttc atcagaaagt cttgttgaga attctgtaag tttcataaaa tctactaaaa
     8161 ccacgtttgt ttttttacgt atagattctc ctaaatcttc tttatttaca ttaaagaaat
     8221 tttttgcttc aataattaat tcttctgatt taggtctctt tttttgtccc tcttctggat
     8281 tacttgctgc agaataatct tcttctgaat aattagtacc atctcccatt aattttagat
     8341 agaaaggggt tttttataaa tttgtgtgta atataaagtg tattctttat ttgtagtttt
     8401 ttaaagcagc tttaacgttt tttagaagtt ggtccatact atcttctagc tgttgcctgt
     8461 tttttgtacc agtacatact aactttccgt ttgagaaaag caggaatgtt gctgtgggct
     8521 caattagttt gtaaactaga cctggaaact gttcaggttc atattctgtg ttttccattt
     8581 ctaatgccaa aaaattaaga tttaatttta attcaatgct tccagatgca actatatttt
     8641 gaacagttat ttttggtttt cctgtgatat tgacacttat tttcttaagt tgttttatta
     8701 cagcatctat aacttctctt acttgggtta aagattttgt acctgtgcaa actaaatttc
     8761 ctgaagagaa aactaaaact gcagatttag gttctttaat tcttaatact agtcctggga
     8821 atgtttctgg attatattct gtgtttggtt gtgttcttgc aagttttgtt aaaggaacag
     8881 gttttcctaa agaagcagtt gctacaatat tttgtatttt gtaattgctt tgtttttgtg
     8941 ccattttaat gatttataaa gagaaaatgg gtttataaag gtttttaaag agttcgtcga
     9001 gaggttttta gtttatttag gccagtattt atcaggaaac ttttcttgat aattttcaga
     9061 ttgtggattt gtttctggtt gttccttaaa attatgggtc tgatcagatc cgtaatattg
     9121 ttctatttgt gaagggtaat tagggtcatt tttatattgt ccttgttctg ttcttggtct
     9181 ctcttttaat ttttctcttc taattattat tctttttaaa ataattacta ttaaaattat
     9241 aactgctatt cctaataaag ttattaaaat atatactaag atattttttc tttcacaagt
     9301 ttgagttaat tcaggttttc ctttttctgt accacaatca tttatatctg tgcatgttct
     9361 cgttcttttt tcttctctag ggcatttttc aggtaaaaac tgagtgcatt tccaattagg
     9421 aatacataca ggacattctt gatattctag aggtttacct gtaatatttc cacaattgtt
     9481 taaatctgta caagttctat atccacatga tttagaagtt tcactccatt catcacactg
     9541 ccaattttga acacaaggaa tttcaggagg gattatttcc aatattgcag agtgttctaa
     9601 tcctgttatt atgaatttat ttcctgagat attacatgtg agatttcttg ctgttgaagt
     9661 taaatttcca ggacattcaa ggatatattc atcaattgca ttacactctg gacttatttc
     9721 atctattgaa tagatgtctt tatcttttat acaaacagat tcagatttgt cgtttaatct
     9781 atctactgtt acatttttgg tatagtttaa tccatttatt ataatataac ctcttgtgga
     9841 atctgtttcc tgttttttaa gaattatttt tgtccaatcc aaacttcctt cttctgtaaa
     9901 gttaatttct gtttctatta ttgtttgttc atcttgattt atttctatcc tctgtattcc
     9961 tgaacgcgat tcggaaaaga gacctatttc ttcagacatt tttaatttag agtcatttat
    10021 ttttacttct atatttttta aagaagtgtt tattggagat gtccaatttc catataatct
    10081 ttctagttct tttgtaaatg ctaaaagtga aatatctgtg tataagtata tattcatact
    10141 ataattttgc aacaccattc cagcttttgc gattattccc atagggtcaa ttgattctcc
    10201 aaagagagtt atattctcta aacttccatt ttcaaaaaag ctaaatgcag ttagattctc
    10261 tgtaattcca tctactttaa attcttcttt tgtccagttt gatgaagaaa tattaaatac
    10321 agattcaaat tctgtttttg cttcttgact ggtgtttata tttcttttaa ttgtcctgtt
    10381 tgctaaaaaa tttaaaagta aatattctgg agcattttcc atctcgtttt gttcagatga
    10441 acttattctt tgtattatat tttccatttc ttctggagga taattgccat ctatggccat
    10501 agccattttc atattcatta ggaaccatac atcaattcca ataagtgttc taatatttgt
    10561 cccatttatt tgatatacct tatatgccgg acacttttca tttaataata aactccaaat
    10621 ttcactttct tcttctgagg aattagtatt attgtatgga gcagaagtaa ttgttaaatt
    10681 ttcagaactt cctttgaaaa taaattccca tacttttttt atgctttcat tggaacaatt
    10741 ttcaggtgtt ggaggatttt ctattacctt tggagttaaa tgattttgta ttacatttgt
    10801 tgttaatact aaatttttaa aaattagaat aaataatatc aaagcaagta atattaagca
    10861 tattagaatt ggaggtattt catcttcttt ttttggtttc ttgcttcctc tttttttcat
    10921 atttttttat taggattttg tgttttaaaa gcttatttat tattaattgc ttttttggcc
    10981 tttaacttct ttttcaattt cttgtaaact tgctatttca gaaagatgtt tatctgggcg
    11041 tagagaagtt attcttggga atcttagagc ccatccagaa ctgtaatttg gagattcttg
    11101 aatttcttga taagttacag caacaactat tttaggtttt attttaacat cttttccttg
    11161 ttcttttatt attaatggtt ttaagaggtc gtttaattca ttgaatgaaa ctccttcttg
    11221 ttcatctttt tcttttatac ctgttccgac tcttccgatt tctaagaatt cttgttcttc
    11281 ttttcccata catgataaga tgaagctact taaccaacca gaacgctttc ctgttccata
    11341 ttctgcacct gttataacta aatctaaatc tttttcagag ggtttaactt ttaacatgtg
    11401 tcctactctg tttcctggtt gatatgctgt tgaaagattt ttcatcataa tcccttcttg
    11461 attgtcttta agtgctttct tgtaaaattc ttctgccttg tttaaatcat cagttataat
    11521 ttgttttgaa gaaacaattt tgtaaggagt ttcttttatt atcttcctta ttaattgtgt
    11581 acgtttttca aaaggttctt tgattaaaga ttttccatta taatataaaa tatcaaaaac
    11641 atttatttca attggaagtt cttctcttat tttttcaata ttatattttc ttcttattct
    11701 ttgagagatt gcttgaaatg gagtgtattc ttttgttttt ttgttatatc ctattgcttc
    11761 actatctaat ataaatgatt ctcctctaac atatttttgg atatattcaa ctgcttctgg
    11821 gaattgttta gttacatttt ctagttttct tgtaaataat tttattttag aatctttttt
    11881 atggattata agtctaaatc catcatattt atattctatt gcacatggtt ttccaactgc
    11941 cttaaatcct tcttcaatgt tttttacttt ttgtgcaagc atcgccttta ttggctttcc
    12001 aacttcaaga cctattttgt caaattcact tatttttcct ttttttgaaa ttaagaatac
    12061 ttttgctaaa tcattggaat ggtcaattgc ttcttgaatt atctttgaag attctttgtc
    12121 tgaattgaaa aatgcctttg cgagtgattc tcttattgtt gattcttgga ttcctattct
    12181 taaatctcca agaagtgttc ttacaatata taatgcttct attggagaag ctgaaactaa
    12241 taattcagtt ataagaatta attttttttc aattgttcct tctccttcaa gctctggtag
    12301 ttttcttaaa ttttcaatta ctttgtctgt tgttagatgg gatttgagaa ataatgttga
    12361 ttgttttttt gtgttaatta gtttttcagc tactttacct aagtctccta tttttttcca
    12421 ttcttgaact actttatcag gagagtttcc agatgctttt gagattgctt ttattacaag
    12481 ttgattactt attccaattt ttctttcatc ataagttgga tatatttctc catttagtaa
    12541 atataatatg tccttgtctt tttcagaaag atattctaaa aattttgaga gaatttcaat
    12601 tttttcaaga cgctttgtgg tatttccaag attttcatat aattctgcaa gttttgaata
    12661 tagcatagat tataatatta caagaagttt ttaattattc ttatttattt tcactttcca
    12721 caacccatgt agattacaat attctcttgc tgaaacaggt ttaaatgaaa attctgcttc
    12781 tggttttgaa tctggtttta gaaatacttt ggcagtctct cctgtttctg aagttgcttc
    12841 aatccattca atgtaatgtt ctgttaccat agggtgggca attgaaccta cttttacttt
    12901 tttaccacga ataattggaa catgtttttc ttgagaagca tcaacagtgt tttctttttg
    12961 caatttcatt ggttgtccac aacagattaa tacacctctt ccttcatgca cgacttcaac
    13021 tatatttcca catatttcac atttataaat ttcttttttt tctaccattt tataggttaa
    13081 taattttctt ctaaaatctc gtaataggat tgaggatgtt tacatgcagg gcattcatca
    13141 ggagcttctt ttccttcatg aatatatcca caatttctac atttccactt tacaatttct
    13201 tctttttgga atactctttt ctcttcaata tttttgagta gttttctgta tcttttttcg
    13261 tgttgttctt caacttctgc aatttcatgg aaagattctg caatgtcttt gaatccttct
    13321 tttaatgcta ctttttcaaa ttcagggtat agttcttccc attcttcctg ttctccattt
    13381 gcagctgcaa gaagattttc ttttgttgtt cctatttttc ctgcaggata acttgctgtt
    13441 atttcaacag gtaatccttg tgatttttct aaatatttaa agaatacttt tgcatgttct
    13501 ttttcattat ctgctgtttc tgtgaatata gctgagattt gttcataacc ttcttttttt
    13561 gcaacagatg caaaataatt atatctcatc cttgcttggc tttcaccagc aaatgccttt
    13621 agcagatttt tttctgtttc acttcctttt aattccataa ttatatttgt ttgttgtagt
    13681 ttaaatagat tatgcaattt tataattatc tatgtaaaat atatcttctt ccagaaggag
    13741 ggtttggttt ttgtccagct cttctacttg gtctgttctt gtttgttgga gatttatctc
    13801 ttaatcctct tgatttctta gctgaacttg ttagtcctct aaagaccctg ttcttgtttt
    13861 ttctatttac tatccaattt attgttttat cattttttat ttctggtctt tgagggtcta
    13921 cacaaataac ttcataaaaa taatttattc catcttttcc gattttataa gaatttaata
    13981 cttcaaggtt agtgtgttta tttgcaactc tttgttctgc aatccatttg taagacatct
    14041 ttagattttt tcgagtgtgc attctttttc ctcttcttcc ttttatgggt cttggccttt
    14101 tgtgtcctcc tctttttact tttactctta taaccacaaa tccttttttg tctttatatc
    14161 ctaaagctct tgctctgtct aatcttaaag gtttttcaac tttaatctga gttggacttt
    14221 ctctccattc tttcatcctt gccataagga cttttgaatc tggttttttc caagctttct
    14281 taatataata atacattcca tgaacacttt tttgaggttc ttgttttgtt tcttttttat
    14341 ttccttttgg tttcttttct gccatgagta ttttagaaaa agttcattta aaaagcttga
    14401 ggttcagggt tttgtaacca cttatctgaa gacataattt agcagaataa tttataaacc
    14461 ccaatagttt ttttagtcta tgaaaaagag tgttgctaaa aaagcagtta agaataaaaa
    14521 aaagaaaaag gcgagacatg aggtaactct taattttctt aaaatatcta aatctgcaga
    14581 agctccagaa tatgctttag atacagatgc aggttttgat ttaaaagctc ttgaaagtgt
    14641 gactatattt ccttttgatc aaaaaaaagt cagtactgga attgcaatag aagttccaga
    14701 aggatatgtt ggaattgtaa gagatagggc tggaattgtt cagaaaatga atgttcacac
    14761 tgttgctgga acttttgata gtggttttag aggagaagtt tcaataatgt tggttaatat
    14821 gaatgataag acaattgaga ttgagaaagg gatgagaata gctcagataa tcttggttcc
    14881 aattgttaag gccaaaataa ctgaagtgaa aaaactttca tctacagaga gaggagataa
    14941 gggctttggt tctacaggta tgaaagagat tataagagaa ttgaataaga tttctgaaaa
    15001 aagcatttaa gtaactttta taaattgttt gagtttgttt ttgttaccag aaatagatat
    15061 gttttggggc tgtagctcag tctggttaga atgtttaaaa cataaaccat cctgagaaga
    15121 tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntagctcag tctggttaga
    15241 atgtttaaaa cataaaccat cctgagaaga tagtcttgtt ataaagcatg aacaaaacat
    15301 aaacgaaaca atgaggctgt agctcagtct ggttagagca ctgctctgat aaggcagggg
    15361 tccacagttc aaatctgtgc agcctcattt aaatttaatt tcctttgaat tcaagaacaa
    15421 gcaacccttt tgaaaattca tatcctatta taggtaagtt taagggtatt gattttgaat
    15481 aagctttttc ttctgcaatt gcttttaatt ctatgctgct ttctaatgga gttattgtaa
    15541 cattttctat tgattcaact cctggcattt ctatttcata gattattttg tcagcaattc
    15601 ttcttaattc tgttttaggt tcttttttat tgagtttaga gaattttttt atttgctctt
    15661 ttgagaattt tttgaatctt aaaggaatag attcttgttt ttctttttta ttttctcctt
    15721 gtttttgcat tccattgttt aaatccactt cttttccatt tatcattaat tttatttttg
    15781 ttttaggaaa attattgttt gttggagatt gtttagacat tctcatttct ttttccattt
    15841 ctttttcaag catccttatt gtatttgaaa tcattttatt catgacattt cccatcattc
    15901 ctccaaacag agaattagaa atcatatctg tttcattgaa agaatcattt tcacctaaca
    15961 ttccaaattc ttcttttttg cctttttttc ctaatgggaa accacagcta ggacaaaagt
    16021 tgtttccttt tcctgttttt tttccacatc gttgacaagt ctttttagag aacatattta
    16081 atttatatga gatttattta aaaggtttgt ggatagcata ttatgtagaa taataatgat
    16141 tatatatgtt gtttttaaag attatttatg gcaattaagg ctattaaaga aatacctttg
    16201 aaatctcggc atgttaggtg gaattttgaa aaatctgaag ataattttta tatggggaat
    16261 cctttgacta gatattactt tgctattata gataataggg aagcaaacag gaaagaactt
    16321 tcaaataaga ttgctaatca attgatgaaa tctaattatt gggttattcc tgttgaacaa
    16381 ggatttaatc ttaaagttaa gaatagtgag tggggtttat cttttgttgt gtatgatgct
    16441 ttagcaagtt atgagaattt gaatttttca aaactaaatg ttgtttcaaa aagagattat
    16501 aaattgtgta agaaagaagt tctcagcatc gaggattatc ttacaagaat ttacgaatag
    16561 tttaattttt tcataaaaaa tggttttaga tgatttggcg aaaagtattt aaatacaatt
    16621 tattttatct ttggtatgaa gaaagaaaag aggttaaaga aatttaatat ttcaaacaga
    16681 cttgcttata cattaatggt tattctttca atcgcattat taggaattgg agtttgggct
    16741 gcagctccaa accctggaca tagtgcaaat gaattggaac tagattgggg ttcaaagagt
    16801 ttttgtgaag gttctgtaaa tccttgtgat tctcttactc ctagccagtg tagttctcaa
    16861 agtacatgtt atatgagttg tactggaagt ggagggtatg taagttgttc tgcgtatact
    16921 gatgagtcaa tatgtaggga tcatgggtgt atgtgggagg gtagtagtag gtattgttat
    16981 gggagtggtg gagaaacagt ttcctgtagt tctttttata gtcagtccga ttgtgatcgt
    17041 tatacgatgg taggttgtta ctgggggtgt tctggaaatg cacattcttg ttctactttt
    17101 actaatccaa cttattgtaa taggcagtct ggttgtaaat gggtaacaag acaaaggtca
    17161 attacaagta acgacagagg aattaatatt tctaatttac aagttaattc cttatgtgat
    17221 atatctggga gtaattgtgt tcaagttaga ggcaaggaag catcgaaaga aggtaactat
    17281 aacactggag acgatagtac aagaagaatg aggataaatc agtatgctag ttgccctaca
    17341 ggatattatg tatctgcagt tcgttcagta tcatatagtg cgagtacttc tgcgttaaga
    17401 gttacatgta ctcctcttcc aataagataa ataaaaatct atttgtttct tatttataca
    17461 aattttttta aactttattt tattatcttt ttttaaataa taattggtaa tatttatgat
    17521 gggagataga gacaataaag aaagcaagga aagcaaggaa aacaatattg agggaattgt
    17581 tgaatttaat gagtggcagg taattgactt gagagttgct caaatcaaaa aagttgaaga
    17641 tatttctggt gcagacaaac tctataaatt aaccttagat gttggtgaag aaataggtga
    17701 gagagttatt tgtgcaggaa ttaaacagta ttattctaaa gaagaacttg aagacaagaa
    17761 aatagttgtt tttgttaatc ttaaacctag attaatgaga ggtattgaaa gccaaggaat
    17821 gcttcttgct gctgtttctg aggaccattc tgaggttgtt ttggtttgtc cagaaaaaag
    17881 tattaagaat ggctgtaaaa taagttaaat taaattgacc tgtttttgtt atttcttttt
    17941 gtagctttat ccttttttca tagtcatttt ccagaaataa gggttttctc ttttcagaat
    18001 tcttctccag aaaggatatc tatctttttt tgagttatat tctgtacttg ttattcttgc
    18061 agcaatttct ttgtaagctc ttgaagcatt tgaatgagga tgagtatgaa caactgcatc
    18121 ttttttgttt aaagcttttt taacagttat gtcttcagga atcattccta aaataggagc
    18181 ttcaagcata tcttttactg attctggact catctctata ctgtcttttc ttactcttgt
    18241 aacaatcact cctaatattg tttttttcat ttgttctgct agttttattg ttttaagggc
    18301 atcagttatt gcaggcattt ctgcatttgt tatgactatt atttcatctg ctagatttat
    18361 tgctgcaatt gattcatctc ctaatcctgc tgaactatca actattatat attcagaaag
    18421 tgtctgaaac tcttttttaa aatcagaaat tttttctggt tttatttttt taagttcttt
    18481 tatagataaa gaagagggca ttacctttaa accagaaaca tgttcatata ttgcttcttt
    18541 tggttcagct tttcctctta aaacatggtt taaattgatt ggtacttctg gagagttcaa
    18601 gtgtaatcca acatttggag ttgataagtt tccatcaatt ataagaacat cttttccaaa
    18661 ataatttata gctgcaccta gatttatagc ggtggttgtt ttacctacac ctccttttcc
    18721 agaagttact acaattattt tgttcatttt gtaacctttt ataagcttct tggcttactt
    18781 tgatagaaga aattgttttg tagtgcttat aaacttttcc agcttttctg catattcctt
    18841 tagtttttcc aaatcaacat ttatttcttt tccttggtag aatatcctta atgctacatt
    18901 tttcctaaat tcgcctattc tctctgttct taattcatct attttcctga acatttcata
    18961 taattcaatt acttctagga aattagaatc ttttttgaat agttttttta tttcttctat
    19021 ttttcctaat ggattcgaag gtattgaact tatttttttc tttttctttg cactttttaa
    19081 tatttcttct atgcttgttt caatcatctt tctccatcta agaataagat ttttaattac
    19141 atcacatgtc tttgtgtatt tcaaacttac atataaaaga tgatctgcgc ttattttttc
    19201 ttggatgatt tcttccatta attgatttgc cctttcccct cctgtttaat cccatttact
    19261 tagaagtgta atttacaagg aggcacttgt ttatatatct tttgctgttc tagaggggtg
    19321 aaagtttttt acttacttaa ttctaaaatt gtttcagcta aatctccatt gtttttttca
    19381 a
//
LOCUS       DAOPCH010000009        19345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_2940_length_19345_cov_3.636651, whole genome shotgun sequence.
ACCESSION   DAOPCH010000009 DAOPCH010000000
VERSION     DAOPCH010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 19345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19345
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2940_length_19345_cov_3.636651"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="PLU55_01180"
     CDS             <1..1170
                     /locus_tag="PLU55_01180"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HPJ86703.1"
                     /translation="KDGKHTRECKNIGDCKGTGTKPIEEMKCSETLFNIILDLSDITV
                     TEDKTIIFKVNFTEVRGKEKIDILIKYSIINEDGYEIFSQIETKAVEGKLEYEKKLSD
                     IKLLNGEYTLKVDALYGNLQRSFAEEKFRVVSGEIIFGERPDEVEPSLSEKFNYKLLF
                     MILGLILILILILIIIFSRRKSAKERGEEVIKQRLKEGWAALDKGDVSEARKKYKDIK
                     SIYNLIDYSNTRVYDKVMKFYDSLKKVSLIGIIILFPLLFAPSVLNKLGFTGNFIGNT
                     PGKISLMFILVLSFFILFLLVFSIGQLTRLVGKKRYENSISSLISKQVYSSNGYLIGK
                     VKEVILKDNKIHSLVLDLDKKYNSSKGIVIEYKAVKNIKSVVIIDEKVIPHLPSF"
     gene            complement(1214..1522)
                     /locus_tag="PLU55_01185"
     CDS             complement(1214..1522)
                     /locus_tag="PLU55_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HPJ86704.1"
                     /translation="MKKVAKEFKEFLNEYKVVPLAIAFIMGAAATALVQAVVNYLIMP
                     ILTPFIPGGAWQTATVTVWKIVISWGALLGAIINFVVIAWVVFLIAKYVFKEQKVSKK
                     "
     gene            1651..2583
                     /locus_tag="PLU55_01190"
     CDS             1651..2583
                     /locus_tag="PLU55_01190"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HPJ86705.1"
                     /translation="MKEIRYPNVPTYKKDGRMPITEIYRKLSRLKESLWIDEVICSQR
                     VDMKEYKRKFLPILGFRTRLKGDALWIIAGIHGEEPAGPNAIAKNIRFLNKLAKKIPI
                     VLIPLANPSGYRRDWRYPTLKRRPKCPNAEVPSVGSSEHVLINPKTGKAQSPKPVNKE
                     AEEISKYVIENFKNYPPLVVLDFHEDESAKKQYIYSHGKLKIYDPIARKVVRILKKQG
                     FKFYGSGKTAFNEKILKGVVADINDGSIDELLSSEKIIVNGKKIKGPDAKSVVVIETK
                     TIGVPLKKRVKAHSYIIKDLKSIYTLAKDIFDEG"
     gene            complement(2637..3506)
                     /locus_tag="PLU55_01195"
     CDS             complement(2637..3506)
                     /locus_tag="PLU55_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPJ86706.1"
                     /translation="MELKKVHYIGLGAGVLILAFSLLFRETKLFMLFVGIGIIAAILP
                     FVLSLMREAREEVEKEEMFLEFTRNLVESVKTGTPISKSIMNLRDKGFGVLGIHIKKL
                     ANQISMGIPLNVALQTFAKDVDNQTVSRTIILIGQAEKAGGDIGEILEAVADAVSISD
                     KLKKERKAAISTLVVQGYIIFLVFMMIILIMQYQILPMVSGISELGSVGTGTFSQVGV
                     SATQVDEGDISDSFFYLLMIQGFFTGLTVGKLGGGGFKSGIKHSFALMLIGFVVSTGA
                     GIILGTPAATPTP"
     gene            complement(3508..4872)
                     /locus_tag="PLU55_01200"
     CDS             complement(3508..4872)
                     /locus_tag="PLU55_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPJ86707.1"
                     /translation="MSRNKEDFQKLREIIAKEKKICKEMIALADYLKRAEVEEQRIIE
                     SQISLLQRTLKKTNDEIPEVLSKINLLVMPTAKSSGPNEDPEKVKQDFTESAKRMKEQ
                     ATLEKNLTKIEEQSSKSEKKGYELKELKPVGLEKLTIKRMKKKEKKVEVKKEKKASMY
                     VKTANKLFSKLSDKIISEKTFMTMERDLVKANLQFTSRGYISVIFLSTLIALFVGAFL
                     GVFFFFFNIGSELPIITKATGDIAGRFLRAIIIFILAPTLTFIFLYTYPGMEKKATES
                     RIDDELPFATIHMSAISGSMIDPTKIFSIMIVTHEYPNLEKEFKKLINEINLYGYDLV
                     SALRNSSYNSPSKKLSELFNGLATTINSGGNLPDFFDKRSQTLLFENRLEQEKKTKAA
                     ETFMDMYISLVVAAPMILMILLMMMKISGLGIQMSTKMITIIMVGAVTLINIVFLGVL
                     QLRK"
     gene            complement(4869..5060)
                     /locus_tag="PLU55_01205"
     CDS             complement(4869..5060)
                     /locus_tag="PLU55_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86708.1"
                     /translation="MSGEGKKEGDQSGSYAFLNKLIITLEEGELKLEEAYKRNNPEQV
                     KAIKEYLIKIYKKIDEEVA"
     gene            complement(5098..6690)
                     /locus_tag="PLU55_01210"
     CDS             complement(5098..6690)
                     /locus_tag="PLU55_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HPJ86709.1"
                     /translation="MAEKVKKEVKVEKKPDTTYIKDYPRPPINFSPVIPPLKRIKDKT
                     QVDVRYCLIAPFAFVHIYWDPELYEVVYDLEEPLLTEEEEKKKEEIIVAMSNMIDHET
                     VVEKEKTKLLEYIDKRLKILAIELGIELSYESYKKIFYYLCRDFVGFNEAEPLLRDYF
                     VEDIECNGANSPVYVVHRVYRNLRTNLVFRDIDRLQSFVEKLAQRCGKYISYAKPILD
                     ASLPDGSRVNATYTKDITSKGPTFTIRKFTKTPWTPPQLIAFGTLSPEILAYLWLLVQ
                     YKMNILIAGGTSAGKTTLLNAIAFFIPPESRVVSIEDTRELNLPRDNWLPSVARNATG
                     VGNSGEISLFDLLRSSFRQNPDYVVVGEVRGAEASVLFQGMASGHSSISTIHADSVDT
                     LIKRLETPPIELSPTLLNVLDCVCIMTHAIVNKEETRKLKEVVEIINVTLDGIALTNT
                     PFVWNALENEFYSKKNSKIFEKIRKRYGIKVEDLEVEFKRRVQLLYKIYQQGIFKFEE
                     VQEIVNEYYKKPQEILKRFEIE"
     gene            complement(6692..7255)
                     /locus_tag="PLU55_01215"
     CDS             complement(6692..7255)
                     /locus_tag="PLU55_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86710.1"
                     /translation="MKKKGDIKRNTFSKNYRGQVWIETVIYLLIAFVMMGLVLSYVKP
                     KIEDLRDKAIIEQSLDVINEIDNTISTIGSTGNKRLIEIGVKKGVFNIDSENDMITFE
                     LETKYQYSEFGKEVYIGKVKAITQGQNQYSKVILTRDFSEEYNLIYDSSDTNKALPKA
                     STPYRVFIENKGVGVDQDKLIINFNIE"
     gene            complement(7303..7884)
                     /locus_tag="PLU55_01220"
     CDS             complement(7303..7884)
                     /locus_tag="PLU55_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86711.1"
                     /translation="MHKMISKKSKNKKALSTVVATVIIILLTMAAITIVWVFTKNIVN
                     KNKDRVESCFEVESSEKVNLNPLYTCYTNKSEVGGDAGFEEVQFSINIADVEIDSLVV
                     AIEFGGESRSIILNNTPVQKSNMKMYNSANNWGVDLIALPDKNAGKTYVVNFDGTTAT
                     KVDLIRIAPVINGEQCGVTDRISNVVNCNLLIV"
     gene            complement(7877..8374)
                     /locus_tag="PLU55_01225"
     CDS             complement(7877..8374)
                     /locus_tag="PLU55_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86712.1"
                     /translation="MKRGVKKGHRKNTRALSPVIATVLLIVIVVIIALIVFFWIKGMT
                     DEAITKFGNRNIQMVCEDVDFEASYTPTTGLYITNFGNVPIFGMDVKEESAGSHTTID
                     LRENPDWPEFGLITGAIFSDPDFEAPGVQEMVLIPVLIGESDSGRKTYVCDERQHGVR
                     ISANA"
     gene            complement(8412..8975)
                     /locus_tag="PLU55_01230"
     CDS             complement(8412..8975)
                     /locus_tag="PLU55_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86713.1"
                     /translation="MKQTKNKLNKKAVSVMVGYVLLIVFAVVLGGIVYAVLKTYVPQD
                     KMECPEGTSLIIESYNCNDNSLEFTIRNNGKFDVGGYYIYATDKLGQKKATINLAKYN
                     LNEYSILTPLRIDGIKLGITPENHILDFENEFAVNSEAQIERYDLSTVDKIYAIEIVP
                     LRWQEEEGRIQTVSCADQKIRKSLECL"
     gene            complement(8992..9726)
                     /locus_tag="PLU55_01235"
     CDS             complement(8992..9726)
                     /locus_tag="PLU55_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86714.1"
                     /translation="MSTLGKENRKKKISKKAMSVIIGYVLLIVIAAVMGVIVYKWQKT
                     YTPQDPHGMCPDESSLMITSLNYDCDNDVLTFELKNTGQFSIGGYFVYVKDNPEKQIA
                     TVDISRNNTDEFSPMLHQLGINGIKLGMDSEDPRLTQENDFLPGEVETERYTMVGVPE
                     PIYTLEIVPIVWKKDKGKIVIASCKEIRIVEKIPGCTQRCIPEDPCGDRECGKMLNNC
                     YQEVTCGDGCGAGEFCDSTGHCVNSL"
     gene            complement(9710..10180)
                     /locus_tag="PLU55_01240"
     CDS             complement(9710..10180)
                     /locus_tag="PLU55_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86715.1"
                     /translation="MKKGDKRGQFYLIAAIIISGILISLAYLANYSTKNVSYEAEEIA
                     KELKIEAEYVLDYELKNDKEVLDDFSMRYSDYARDKEIYYIVVDNNPATPVKEAYMFN
                     GNQKIILNDRLFVGPKTIEFNLDEKTYSFPKEEGKNFYFVIIYDKGGERYVYTG"
     gene            complement(10197..11333)
                     /locus_tag="PLU55_01245"
     CDS             complement(10197..11333)
                     /locus_tag="PLU55_01245"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPJ86716.1"
                     /translation="MGEKKSNMTVNPYEVKGNVDYGRLINEFGLEKIDEDILKKIKKH
                     TKNELHPMLRRGIFFAGRDLKWLLGEYEKGNKFFIYTGCGPSGPIHIGHLMVWYFTKW
                     LQDKFDCELYFQFTDDEKFLYKDLSYEEVQKWTEENMLDVAAIGFNPKKTHFIIDTKH
                     AGIMYPEAVKIAKKITFSTIKSSFGFNDSNNIGSIFYTAMQAVPAILPSILKKKNIPC
                     LIPHAIDQDPHFRITRDILPKLGYYKPASIQCSFLPPLTGFSGKMSSSSEGVKSILST
                     DNPAEVKNKINKYAFSGGRDTIEEHRKLGGNPEIDVSFQYLKHFFEEDDKKLAQIEKD
                     YKSGKLSTGELKKYTIEKINKFLGEHQERRKKAKKLVSQYILKT"
     gene            complement(11389..11676)
                     /locus_tag="PLU55_01250"
     CDS             complement(11389..11676)
                     /locus_tag="PLU55_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86717.1"
                     /translation="MKKYVTYDEEEMLRNLNSIYRSLDNLAKYLALVSQQLDYKRSNL
                     TSKYLKESVSTELSNITSILNNFNLESEKKETPSIEKKIKELEEKMKQDID"
     gene            complement(12126..12395)
                     /locus_tag="PLU55_01255"
     CDS             complement(12126..12395)
                     /locus_tag="PLU55_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86718.1"
                     /translation="MDDEGFWDDEYERFWEQEFRKLDFSPAGRRLEHKIAELYQKEIS
                     KLDASDKKKKELTDMIKDYILCYEMEYARYYQAFVFYGLPYPELN"
     gene            complement(12430..13212)
                     /locus_tag="PLU55_01260"
     CDS             complement(12430..13212)
                     /locus_tag="PLU55_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HPJ86719.1"
                     /translation="MVRKRENILIFFAFALLMLLGNVYATSVCGNGILEEPYEECDDG
                     NNIDGDGCSALCKIEFCGDMICNNYETVFNCPCDCGDCPIIKFCGDGICSPSEGENCA
                     TCPEDCGECPTSENKYCYSCPVGECNLNENCGNGIIEEGEECDDGNTKNFDGCSKVCF
                     IEKEEGYGEKAIKKNHFVQYCEPNWKCSSWGECVHGTTTRKCIDENRCSYSYNKPIET
                     TSCVDEILSPVYLENKSVRNVWILIQLIIFFILLGIVIKLLL"
     gene            complement(13281..17318)
                     /locus_tag="PLU55_01265"
     CDS             complement(13281..17318)
                     /locus_tag="PLU55_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HPJ86720.1"
                     /translation="MKNRKHKVLLSLVVVFLMFVTIGSVAAKDIAYITRGASYTDPAI
                     INILNNANYTYDVIYQNDLSLIDFSKYSVIIVGEGNFNNPSLIPVNQKNSVIFNSYHL
                     GTWGWTGRSVSTKASNYPSEIYVRDSESLITQNVPDAFIPYYKDNVMISYRLKYISRF
                     YPAPGLEVMVTDKPNYFNGAIVAAINNGSALRGGQVSNARGVFIGFTETNLLTPDAEE
                     IIYNSIEWAMGGEDRDGDGFFSDEDCNDKDPNINPDAEEIPYDGIDQDCDGSDLNDLD
                     GDGFIAVIAGGDDCDDDNPLINPGSSDPYMNCVNDAPIIEPISKISVHEGEIVEVYII
                     AYDAEEDEITYEINDSRFVQDEEDPTHFSWQTGYEDEGDYSFFVLTSDGELTSSKRFS
                     VKVWNRNKAPELIMDIPEQEWDEDTTHTLDLTEYFYDLDGDKLYYLFSESSDNSNIIL
                     EKIEGGVAHFKSKKDWSGEDWIIFKVTDGLNVVYSNNITLRVLPVNDAPVILGNIEVI
                     NMYEDGVYELILEGLVYDPDSELEYSFENTSHITLQLSEDLFSISIIPEENWYGEEEV
                     EIIVNDEEFQVELKFIVKVNSVNDAPIIEDIEEQFVLAGDTLSFSVFASDIEGDEITY
                     EINDSRFVSDENNFEWETTEEEFGIYEFLIGAYDGEDYGYKTVKVNVMQKIVINEIVT
                     GSEGWVELYNPEEIPFSMNNCFISNGIEELEMYGRLNNKGFAAFGWNALQNSGYIELR
                     CSNIIVNRVEYENFNSGFNSIVSLARIPDGSDNWEILYYPTKGVSNLDDVTKPEVELD
                     SPVENTLFTENREVLFEFYVRDNMAEEIKCSIIVNDRILETSKFENDTLGSFYIDYLQ
                     DGIHSWNIKCDDGSNTNTATEDWVINISAPDNPVLNKIGNKVTSEGNELKFIVSATDQ
                     DKDPITLYTNKLPEGASFVDNGDGKGTFTWIPNYNQSGSYKIEFTAEDNTGLKDSEEI
                     TILVGNTKEPPKFSDAETCSSKDKDDLIEITIKEPSKGDNFEIGEVIKGKIRLKNRLK
                     DDVDFDVEIHLYDVEEEESIESIEKSLDIDRGKSGEIEFEFKIPTDVESKEFAIYVYV
                     ESEDGECNSNYVEIDVKRKKHEVVISEMTLNRESASPGDEIEVRIKAENLGRGDEDIR
                     IIVEIPSLNILEKSEEIEIEKYGDDNKETKTFYISIPQQASYGKYEIKATVIYKDGED
                     SKTEKFEIVSGTKKEIITQNAPINLNGNIVIAGSASGSSGKPLNLGSTSDSSTTTKTP
                     VVLKSTSNTKSLRLFPTTSSVNLGIKEKPSKEYNPQLKVEFDRQGAKKPLNAERYQWI
                     LLSIVLAAVIIIVFVLIVYIRKNPY"
     gene            complement(17321..17932)
                     /locus_tag="PLU55_01270"
     CDS             complement(17321..17932)
                     /locus_tag="PLU55_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86721.1"
                     /translation="MRNKKTLVFAFAIVVLMTGITMISAVTGSIGNGRMVLYPEVNGW
                     TNTVIPKTILVNNVNSIPVNITLRLDSNASKFIELVDETFILEAGESKKAEITIKVKK
                     EGTYEGRVNVFFKPVEGKEAGVVLSSTIIVIAKKDQGYKEKEEPKESQEETNPENPGE
                     QTNPSKLVKIFGMNTLILFVVLLILIYIWGKKRKKSKRKVRRK"
     gene            complement(18016..18894)
                     /locus_tag="PLU55_01275"
     CDS             complement(18016..18894)
                     /locus_tag="PLU55_01275"
                     /inference="COORDINATES: protein motif:HMM:NF021615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HPJ86722.1"
                     /translation="MEKQKRGDEDKFTFGSLFDKSVNEYKRNFKPILKLVLIFLGIIL
                     VLAFLFNSITLITDERIFNIMSNPLLIGQYNQGTIKLPIYYDIVSISLNIVYFFLALF
                     VGVGLIAVSLKKDKFSYKEILENGKTNYWRYFGFTIVVTIFIMLLFLLLIIPGVIFAV
                     YWVFAAYVFLEKKQKIIDSLKESKRIVKKRWWKTFGYMILFGLVTLAFVLILSIIKLP
                     IGIPVLLKTISSSNISLGLLLGSLTLNLIYDFVIALVVTPMFILFLKNFYFEMKNSVK
                     ESPKKDLESARKKKKR"
     gene            complement(18963..>19345)
                     /locus_tag="PLU55_01280"
     CDS             complement(18963..>19345)
                     /locus_tag="PLU55_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86723.1"
                     /translation="DMFISGTDFYDSSSSGAKCPTTNKLDLSNFAYYATNGAYSTKQD
                     MEVGRTCDDEGYCSLNYGTGFNDPFRFYNRNEIIQANNNYGYYFSNILSPGAEMTLIF
                     RLNLPEPCNGDFDTGQIYFWGEAI"
BASE COUNT         6226 a         3469 c         2372 g         7278 t
ORIGIN      
        1 aaagatggaa aacacacaag agaatgtaag aatataggcg attgtaaagg aacaggaact
       61 aaacctattg aagaaatgaa atgttctgaa actttattta atattatttt ggatttaagt
      121 gatataactg taacagaaga taaaacaata atctttaagg taaattttac agaggtgagg
      181 ggtaaggaga agatagatat tctcatcaaa tattctataa taaatgaaga tggatatgaa
      241 atatttagtc aaatagaaac aaaagcagtt gaaggaaaat tagaatatga gaaaaaatta
      301 tcagatataa aactcttaaa tggagagtat acacttaaag tagatgcact ttatggaaat
      361 ttacaaaggt catttgcaga agaaaagttc agagttgtta gtggggaaat aatttttgga
      421 gagagaccag atgaagtaga accttcttta tcagagaaat ttaattacaa acttcttttc
      481 atgattttgg gattaattct aattttaatc ttgatattga ttataatatt ttcaagaaga
      541 aagagtgcta aggagagagg agaagaagtt attaaacaaa gattaaaaga aggatgggcg
      601 gcattggata aaggggatgt tagtgaagct agaaagaagt ataaagatat taaatccatt
      661 tacaatctta ttgattattc aaatacaaga gtttatgata aagtaatgaa attctatgat
      721 tctttaaaga aagtatctct tattggaata atcatcttat ttccattatt gtttgcacca
      781 agtgtcttaa ataaactagg atttactggt aatttcatag gaaacactcc tggaaaaata
      841 agtttaatgt tcatcttggt attatcattt ttcattttat tcttattagt attctctata
      901 ggccaattaa ccagattagt aggtaaaaag agatatgaaa attccataag ctctcttatc
      961 tcaaaacaag tttatagttc aaatggatat ctaataggaa aagtaaaaga agttatatta
     1021 aaggataata aaattcacag cttagttcta gatttagata aaaaatacaa ttcttctaaa
     1081 ggaattgtaa ttgaatacaa agcagtgaaa aacattaaga gtgttgtgat tatagatgag
     1141 aaagtaattc ctcatttacc tagtttttag tttcctctaa gatttaaaaa ataaaaaaaa
     1201 gaaattgttt tgtctacttt ttagagactt tttgttcctt aaatacatat tttgcaatca
     1261 aaaacacaac ccatgctatg actacaaaat ttattatagc acctagaagt gctccccaac
     1321 ttataacaat cttccataca gtaactgttg cagtttgcca agctccacct ggtataaaag
     1381 gggttagtat aggcattatt aaataattaa caacagcctg gactagtgca gtagcagcag
     1441 cacccattat aaaagcaatt gcaagtggta cgactttgta ttcattaagg aattccttaa
     1501 attctttcgc cacttttttc atatatttgt tacttaaatt ctatttaaaa acattatctt
     1561 ttgcatttta atctcgagga gaaatttaaa aacatttatt aatgattttt tctatctcta
     1621 atcaaatcta aagaattatg gggtgtttat atgaaagaaa ttcgttatcc aaatgttcca
     1681 acttataaga aagatggtag aatgcctata acagagattt acagaaaact ttcaagactt
     1741 aaagaatctc tttggataga tgaagttatt tgttctcaaa gagtggatat gaaagaatat
     1801 aaaagaaaat ttctccctat tttaggtttc agaacaagac ttaaaggaga tgcactctgg
     1861 attattgcag gaatacacgg agaagaacct gcaggaccta atgcaattgc aaaaaatatc
     1921 agatttctaa ataaattagc aaaaaaaatt ccaatagtcc ttattcctct ggctaatcct
     1981 tcaggatata gaagagattg gcgttaccct actcttaaaa gaagacccaa atgccctaat
     2041 gctgaagttc caagcgtggg ttcttcagaa catgttctta tcaatcctaa aacaggaaaa
     2101 gcccaatctc caaagccagt caacaaagaa gcagaagaaa tatctaaata tgttattgag
     2161 aattttaaaa attatccacc attagttgtt ttggattttc atgaggacga gagtgcaaaa
     2221 aaacaatata tttattctca tggaaaatta aaaatttatg accctattgc aagaaaggtt
     2281 gtaagaatct tgaaaaagca aggatttaaa ttctatggaa gtggaaaaac agctttcaat
     2341 gaaaagatat tgaaaggagt tgtagcagat ataaatgatg gttcaataga tgaattgctt
     2401 tcttcagaaa aaattattgt taatggaaag aaaataaaag gaccagatgc aaagagtgtt
     2461 gttgtaatag aaacaaaaac aattggtgtt cctcttaaaa aacgtgttaa agcccattct
     2521 tatataataa aggatttaaa atcaatttac actctcgcaa aagatatttt tgatgaaggt
     2581 taagattatt aattccttat aaaaatctaa ttaattctca tacaaataaa ttttatttaa
     2641 ggtgtaggtg ttgctgcagg tgtacctaaa attattccag ctcctgtgga tacaacaaat
     2701 cctattaaca tcaaggcaaa tgagtgcttt ataccagatt taaatcctcc tccacctaat
     2761 ttacctacag ttaatcctgt aaagaaaccc tgtatcatta aaagataaaa gaatgaatca
     2821 gaaatatctc cttcatcaac ttgagttgct gacactccaa cttgagagaa agtacctgtt
     2881 ccaactgagc ccaactcaga aattccagat accattggaa gaatctgata ctgcattatc
     2941 aagataatca tcataaaaac aaggaaaata atatatccct ggacaactaa tgttgaaatt
     3001 gcagcttttc tttctttctt aagtttatca gatatactta cagcatctgc aactgcttca
     3061 agtatttcac caatatctcc tccagctttt tcagcctgtc ctataagtat aattgtcctt
     3121 gatactgttt gattatctac atcctttgca aatgtttgaa gagcaacatt taaaggaata
     3181 cccatagaaa tttgattagc taatttttta atatgaattc ccaaaacacc aaacccttta
     3241 tctctcagat tcataatact cttacttatt ggagttcctg tttttacact ctcaactaaa
     3301 tttcttgtga attctaaaaa catttcttct ttttcaactt cttctcttgc ttctctcata
     3361 agagacaata caaatggaag aattgcagca ataataccaa tacctacaaa caacatgaat
     3421 aatttcgttt ctctgaataa caaagagaaa gctaggatta aaactcctgc tcccagtcca
     3481 atgtaatgta ctttttttag ttccatttta tttccttaat tgcaatactc ctaaaaatac
     3541 tatatttatc agtgtgactg cacctaccat tattattgtt atcatctttg tactcatttg
     3601 tattcctaat ccacttattt tcatcatcat aagtaaaatc attaatatca taggtgctgc
     3661 aacaacaaga ctaatataca tatccataaa tgtctctgca gcctttgttt tcttttcttg
     3721 ttctaatctg ttctcaaata acaatgtttg tgacctttta tcaaagaaat ctggaaggtt
     3781 tccaccggag tttattgttg tagcaagccc attaaataat tctgaaagtt ttttacttgg
     3841 agaattataa gagctgttcc ttaatgcact cactaagtca taaccatata aatttatttc
     3901 attaataagt ttcttgaatt ctttttcaag attaggatat tcatgtgtta ctatcataat
     3961 cgagaatatc tttgtagggt caatcattga accagagata gctgacatat gaattgttgc
     4021 aaaagggagt tcatcgtcta ttcttgattc agttgctttc ttttccattc caggataagt
     4081 ataaaggaaa atgaaagtca aagtaggagc caatataaat ataattattg ctctcaagaa
     4141 tcttcctgca atatctcctg tagctttagt tataatagga agttcagaac ctatattgaa
     4201 aaagaaaaaa aacactccaa gaaaagcacc aacaaaaagc gcaattaaag tagataaaaa
     4261 tataacagaa atatatcctc ttgaagtaaa ttgaagattt gctttaacca aatctctttc
     4321 catagtcata aaagtttttt cacttattat tttgtcagat aatttagaga aaagtttgtt
     4381 tgctgtttta acatacatac ttgccttttt ttctttttta acctctactt tcttttcttt
     4441 tttcttcatc ctcttaatag ttaatttttc aagtccaaca ggtttcagct cctttaactc
     4501 atatcctttc ttttcagatt ttgaactttg ttcttctatt ttagttagat ttttttcaag
     4561 agtagcttgt tccttcattc tctttgcact ctctgtaaaa tcttgtttta ctttttcagg
     4621 gtcttcatta ggaccagaag attttgctgt aggcataacc aaaagattta tcttagataa
     4681 cacttcagga atttcatcgt tggttttttt aagagttctt tgcaacaaag aaatttgtga
     4741 ttcaattatt ctttgctctt caacttctgc acgtttcagg taatctgcta atgcaatcat
     4801 ctctttgcaa atcttttttt ctttagcaat tatttctctt aatttttgaa aatcttcttt
     4861 gtttctgctc atgctacttc ctcgtctatt tttttataaa tcttgattaa atattccttt
     4921 atagctttga cttgctctgg attatttctc ttgtaagctt cttcaagctt caattctcct
     4981 tcttcaagag ttataattaa tttatttaga aaagcataac ttccagattg atcaccttct
     5041 ttttttcctt caccactcat gtaagattga tgtaaattgt attattaaat attttgatta
     5101 ttcaatttca aaccttttta gaatttcctg aggtttttta taatactcat ttactatctc
     5161 ttgtacctct tcgaatttga aaataccttg ctggtaaatc ttgtatagca actggactct
     5221 tcttttaaat tcaacttcta aatcttcaac tttaatacca tatctttttc ttattttttc
     5281 aaatattttg ctgtttttct ttgaataaaa ctcattttca agagcattcc atacaaaagg
     5341 ggtgtttgta agtgcaattc catcaagagt tacatttatt atttcaacaa cttctttcaa
     5401 ttttcttgtt tcttctttat ttacaatcgc gtgagtcata atacacacac aatcaagaac
     5461 attaagcaat gtaggcgata attcaattgg aggtgtctcc aatcttttaa tcagagtatc
     5521 aacagaatct gcgtgaattg tacttataga tgaatgtcct gaagccattc cctggaatag
     5581 aactgatgct tctgcacctc taacttcacc tacaactaca taatctgggt tctgtctaaa
     5641 tgagcttcta agtaaatcaa acaaacttat ttcacctgaa tttccaactc ctgtggcatt
     5701 tcttgcaaca ctaggtagcc agttatctct aggtagattt aattctcttg tatcttcaat
     5761 tgatactact cttgattctg ggggaataaa gaatgctatt gcattaagta atgttgtttt
     5821 acctgcagaa gtacctcctg caataagaat attcatcttg tattgcacta aaagccataa
     5881 ataagcaagt atttcaggac ttaatgttcc aaatgcaatt agttgaggag gagtccaagg
     5941 tgtttttgta aactttctga ttgtgaatgt tggaccttta cttgtaatat cttttgtata
     6001 tgttgcatta actctcgaac catctggaag acttgcatct aagattggtt ttgcatatga
     6061 aatatatttt ccacatctct gagctaattt ttcaacaaaa ctctgtaacc tatctatatc
     6121 tctgaataca aggtttgtcc ttaggtttct ataaactcta tgtacaacat aaacaggaga
     6181 atttgcacca ttacactcaa tatcttcaac aaaataatct ctaagcaaag gctctgcttc
     6241 attaaaccct acaaaatctc tacacaaata atagaatatt tttttgtagc tttcatatga
     6301 aagttctatt cccagttcaa ttgcaagtat tttcaatctt ttatctatgt attctaagag
     6361 cttggtcttt tctttttcaa caactgtctc atggtcaatc atattgctca tagcaactat
     6421 tatttcttct ttctttttct cttcttcttc agttaaaaga ggctcttcaa ggtcatatac
     6481 aacttcatat aattcagggt cccaatatat atgcacaaat gcaaaaggag ctatcaaaca
     6541 atatcttaca tcaacctgtg ttttgtcttt tattcttttt aatggaggaa ttactggact
     6601 aaaatttatt ggaggtcttg gataatcttt tatgtaagtc gtatctggtt tcttttctac
     6661 tttcacttct tttttaactt tctctgccat tttattctat attgaaattt ataatcaatt
     6721 tatcttggtc tactccaact cctttatttt ctataaatac tctgtaagga gtagatgctt
     6781 ttggtaaagc tttatttgta tctgaactat cataaatcaa attatattct tcagaaaaat
     6841 ctcttgtaag tatgactttg ctatattggt tttgtccctg agttattgcc tttacttttc
     6901 caatataaac ttcttttcca aattcagaat attgatattt tgtttctaat tcaaatgtaa
     6961 tcatatcatt ttctgaatct atattaaaca cccctttctt aacacctatt tcaattaatc
     7021 ttttgtttcc agtgcttcct attgtagata tagtgttgtc aatctcattt attacatcta
     7081 aagattgttc aattattgct ttatctctta aatcttctat ttttggtttt acataagata
     7141 gtactagtcc catcataacg aatgctatta aaagataaat aacagtttca atccatacct
     7201 gtcctcggta attttttgaa aatgtatttc tctttatatc cccctttttt ttcatattgt
     7261 attaactaaa tcttggttta taatattttc tttgtgtatt atttacacaa ttaacaaatt
     7321 gcaatttaca acattactta ttctatctgt aactccgcat tgttcaccat ttattactgg
     7381 agctattctt attaagtcca cttttgtagc agttgttcca tcaaagttta caacatatgt
     7441 ctttccagca tttttatctg gaagtgcaat taaatctact ccccaattat tagctgaatt
     7501 atacatcttc atattgctct tctgtacagg tgtattgttt aatattatac ttcttgattc
     7561 tcccccaaat tcaattgcca caacaaggga atctatctcc acatctgcta tattaattga
     7621 gaattgaact tcttcaaaac cagcatctcc tcctacttca cttttatttg tataacaagt
     7681 ataaagaggg ttcaaattaa ctttttcaga agattcaact tcaaagcaag attcaactct
     7741 gtctttattt ttattgacta tattctttgt aaaaacccaa actattgtta tagccgccat
     7801 agttaaaaga ataattataa ctgttgctac aactgttgaa agagcttttt tatttttgga
     7861 ttttttgctt atcattttat gcatttgcag aaattcttac tccgtgttga cgttcatcac
     7921 aaacataagt ttttcttcca ctatcagatt cccctattaa aacagggatt aaaaccattt
     7981 cctgcactcc aggggcctca aaatcaggat ctgaaaatat tgcaccagta attaatccaa
     8041 attctggcca atcaggattt tctctcaaat caattgtagt gtgacttcca gctgattctt
     8101 ccttaacatc cattccaaat atagggacat ttccgaagtt agttatatac aatccagttg
     8161 tgggagtata gcttgcttca aaatcaacat cttcgcaaac catttgaata tttctgtttc
     8221 cgaattttgt tattgcctca tcagtcattc ctttaatcca gaaaaaaact ataagtgcaa
     8281 ttataacgac gataacaata agaagaacag tagcaattac aggtgaaaga gctctggtat
     8341 tctttctatg cccttttttt actcctcttt tcatattaat attaccattt ctgtattttt
     8401 aaattctttg attacaagca ctctaggctt tttcttattt tttggtcagc acaactcaca
     8461 gtttgtatcc ttccttcttc ctcctgccat ctcaaaggaa ctatttctat tgcatatatt
     8521 ttgtctactg tacttaaatc atatctttct atttgagctt cactgttaac tgcgaattca
     8581 ttctcaaaat ccaagatatg attttcaggt gttattccta attttattcc atctattctc
     8641 aatggagtga gtattgaata ttcatttaaa ttatatttag ctaaatttat tgttgctttt
     8701 ttttgtccca acttatctgt tgcataaata taatatcccc caacatcaaa tttaccatta
     8761 ttccttattg taaattccaa actattatca ttacaattat aactttcaat tataagtgaa
     8821 gttccttctg gacattccat tttatcttga ggaacatacg tctttaaaac agcataaaca
     8881 attcctccaa gcacaacagc aaatacaatt agcaaaacat accctaccat aacactaact
     8941 gcttttttat ttagtttatt ttttgtttgt ttcatttttt atattatata cttaaagaga
     9001 atttacacaa tgtcctgtag aatcgcagaa ttctcctgca ccacaaccat ctccacaagt
     9061 aacttcctga tagcaattat ttaacatttt tccacactct ctatctccac atgggtcttc
     9121 aggtatgcac ctttgtgtgc atcctggaat tttttcaact attctgattt ctttgcaact
     9181 cgcaattaca atttttcctt tatctttctt ccagactatt ggaactattt caagagtgta
     9241 tattggttct ggaactccaa ccatagtgta tctttcagtt tctacttctc caggtaaaaa
     9301 atcattttct tgagttaaac gagggtcttc agaatccatt cctaatttta ttccatttat
     9361 acctaattga tgtaacatag ggctaaattc atctgtatta tttctagaaa tatctactgt
     9421 tgctatttgc ttttctggat tatctttaac ataaacaaaa tatcctccta ttgagaattg
     9481 ccctgtattt ttaagttcaa aagttaatac atcattatca caatcataat ttaaacttgt
     9541 tatcataagt gaagattcat ctggacacat tccatgaggg tcttgagggg tgtaggtttt
     9601 ctgccattta taaacaataa ctcccattac agcagcaatt acaattaaca gaacatatcc
     9661 tattatcaca ctcattgctt tttttgaaat ttttttcttt cgattctctt tacccagtgt
     9721 agacatatct ttcacctcct ttgtcataaa taatcacaaa ataaaagttt ttcccttctt
     9781 cttttggaaa agagtaagtc ttttcatcca gattaaattc aattgtttta ggacctacaa
     9841 ataatctgtc atttaaaatt attttttggt ttccattaaa catatatgct tctttaacag
     9901 gtgttgcagg attattatca acaacaatat aataaatttc tttatctctt gcataatcag
     9961 agtatctcat tgagaaatca tcaagaactt ctttatcatt ttttaattca taatccagaa
    10021 catattcagc ctctattttt aactcttttg caatttcttc tgcttcatat gaaacatttt
    10081 ttgtagaata attagctaaa tatgcaagac ttattagaat ccctgaaatt attattgctg
    10141 caattaagta aaattgacct cttttatccc cttttttcat ttgtatacaa tgtttcttat
    10201 gtttttaaaa tatactgaga aacaagtttt ttggcttttt tccttctttc ctgatgttct
    10261 cccaaaaatt tgtttatttt ttctatagta tattttttta attcacctgt agataatttt
    10321 cctgatttgt aatctttttc tatttgtgca agttttttat catcttcttc aaaaaaatgt
    10381 ttaagatatt ggaaagaaac atcaatttca ggatttcccc caagttttct gtgttcttca
    10441 attgtatctc ttcctcctga aaatgcatat ttgtttattt tgtttttaac ttcagcagga
    10501 ttgtcagttg ataagatact tttaacacct tcactgctac tgctcatctt tccactaaaa
    10561 ccagtgagtg gaggtaaaaa agaacattgt atacttgcag gcttataata tcctaatttt
    10621 ggaagtatat ctcgtgttat tctgaaatga gggtcttggt caattgcatg aggaatcaag
    10681 caaggaatgt ttttcttttt cagaatagaa ggaagaattg caggaacagc ctgcattgct
    10741 gtatagaaaa tgcttccaat attattgctg tcattaaacc caaaacttga ttttattgtt
    10801 gagaaagtta ttttctttgc tattttaact gcttcaggat acattattcc tgcatgtttg
    10861 gtatcaataa taaaatgagt ttttttagga ttaaatccta ttgctgcaac atccagcata
    10921 ttttcttctg tccatttttg gacttcttca taacttaaat ctttatacaa aaatttctca
    10981 tcatctgtaa actgaaaata taattcacaa tcaaatttat cttgcaacca ttttgtgaaa
    11041 taccaaacca ttaaatgtcc tatatgaatt ggtcctgaag gtccacatcc agtataaata
    11101 aaaaatttgt tgcctttttc atattctcca agcaaccatt ttaaatctct tccagcaaag
    11161 aagattcctc ttcttaacat aggatgaagt tcattctttg tgtgcttctt tattttctta
    11221 agaatatcct catcaatctt ttctaaacca aattcattaa ttaatctccc ataatctaca
    11281 tttcctttaa cttcataagg attaacagtc atattgcttt tcttctcacc cattcttata
    11341 aataataaga actaatttat taatgttttt atttaaagaa aaattgattt aatcgatgtc
    11401 ttgtttcatt ttttcttcta attcttttat tttcttttct atacttggag tttccttttt
    11461 ttctgattct aaattaaaat tatttaatat agatgtaata tttgaaagtt cagtagaaac
    11521 actctcttta agatattttg aagttaaatt gcttctctta taatctaatt gctgagaaac
    11581 cagtgccaga tactttgcaa gattatctaa acttctataa atactattta agtttcttag
    11641 catctcttct tcatcatagg ttacatattt tttcatgtct tcccaaacta tttttcattt
    11701 ataagcatta tagtcgtaaa aagtatattt tatcaaatat atgcaagatt aatttttatc
    11761 aataattaaa tatctcttca tctaatcaca aaattatttt agaataattt cagtgtagac
    11821 aagtggttag ctactaaggg cctcaaagaa ttgccttcat tcttcctcag ctctgcaacc
    11881 cgctaagcat tccaataggt gtttagggct gctccgatca aggcctgacc cagttcgcat
    11941 ctacgaaatc aaaacggaca aagcgtcaac gtaggaatca agattccttg acacgcttat
    12001 cacgccccac tcgtagtctg ccataaagcc cgtttgcaag cagcggaggg ctaacccgcc
    12061 gacatagtct acaaaaattc aagataatta tcatttaaat tacttttggt ttgattcttt
    12121 caagattaat tcaattctgg ataaggtaat ccatagaata caaaagcttg gtaatatctg
    12181 gcatactcca tttcataaca caaaatataa tcttttatca tatctgtaag ttccttcttt
    12241 tttttatctg atgcatcaag tttacttatt tctttttgat ataattctgc tattttgtgt
    12301 tcaagtcttc ttcctgcagg agaaaaatcc aattttctga attcttgctc ccaaaatctt
    12361 tcatattcat catcccaaaa tccctcatca tccataaata aatctagaaa actacctata
    12421 tatttttttc taaaggagta attttattac aattcctaaa agaataaaga atattattaa
    12481 ttgaattaat atccagacat ttctaacaga tttattttct aaataaacag gagaaaggat
    12541 ttcatcaacg caggaagttg tttcaattgg tttattgtaa gaataagaac atctgttctc
    12601 atcaatacat tttcttgttg ttgttccatg aacacactct ccccaagaag agcatttcca
    12661 gtttggttca caatattgga caaagtgatt cttttttatt gctttctcac catatccttc
    12721 ttctttttca ataaaacata ctttagaaca tccatcaaaa ttctttgtgt ttccatcatc
    12781 acactcttct ccttcctcta ttattccatt tccacaattc tcatttaaat tacattctcc
    12841 aacaggacaa ctataacaat acttattctc tgaggttgga cactctccac aatcttcagg
    12901 acaagttgca cagttttctc cttcagatgg actacatata ccatctccac agaatttaat
    12961 tataggacaa tccccacaat cacaaggaca attaaatact gtttcataat tattacatat
    13021 catatctcca cagaattcaa ttttacaaag agcagaacat ccatctccat caatattatt
    13081 tccatcatca cattcttcat agggttcctc caatatccca tttccacata cagaagtagc
    13141 ataaacattt cctaatagca taagtagtgc aaatgcaaaa aatatgagta tattttccct
    13201 ttttctcacc ataaaattct atagttaaaa ccgcttaaaa gcatataggt tataaagaag
    13261 ataagttatt ttacttgtat tcagtaagga ttttttcgta tatatacaat taaaacaaat
    13321 acaataatta taacagcagc aaggacaatt gaaagtaaaa tccattggta tctctctgca
    13381 tttaatggtt tctttgctcc ttgcctatca aattcaactt taagctgagg attatattct
    13441 tttgaaggtt tttcctttat tcctaaatta actgaacttg tagttgggaa taatcttaaa
    13501 ctctttgtat tgcttgtgct cttaagaaca acaggagttt tagttgttgt gcttgaatca
    13561 cttgttgaac ctaaatttaa tggttttcca gaagaaccac ttgcactacc tgcaattaca
    13621 atatttccat ttaaattaat aggagcattt tgtgttataa tctctttctt tgttcctgaa
    13681 actatttcaa atttttctgt tttactgtct tctccatctt tgtatataac tgttgcttta
    13741 atttcatact ttccataaga agcttgctga gggatggaaa tgtagaaagt ttttgtttct
    13801 ttgttatcat caccatattt ctctatttct atctcctcgc tcttctcaag tatatttaac
    13861 gaaggtattt caacaattat gcgaatatct tcgtctcctc ttcccaggtt ctccgcttta
    13921 atccttactt ctatttcatc acctggagaa gcactctctc tattaagagt catttcactt
    13981 ataacaacct catgcttctt ccttttaaca tctatttcaa cataatttga attgcattct
    14041 ccatcttcac tctcaacata aacataaatt gcaaattctt tactctcaac atctgttggt
    14101 attttgaatt caaactcaat ttccccagat tttcctctat caatatctaa actcttttca
    14161 atagattcaa tgctttcctc ttcttctaca tcataaaggt gtatttcaac atcaaaatcc
    14221 acatcatctt ttaatctatt cttcaacctt atttttcctt ttattacttc tcctatttca
    14281 aaattatctc ctttactagg ttcttttatt gttatttcaa ttaaatcatc tttatcttta
    14341 gatgagcatg tttcagcatc agagaattta ggaggttctt ttgtgtttcc aactaatatt
    14401 gttatttctt cagaatcttt taatccagta ttatcttctg cagtaaattc aatcttgtat
    14461 gaaccagatt gattgtaatt cggtatccaa gtgaatgttc cttttccatc tccattatca
    14521 acaaaactcg ctccttcagg taatttgttg gtatataatg ttattgggtc tttatcttgg
    14581 tctgtagcac tcacaataaa tttgagttca ttaccttcac ttgtaacttt atttcctatt
    14641 ttgtttaata caggattatc tggagcactt atattaatca cccaatcctc tgtagctgta
    14701 tttgtgtttg agccatcatc acatttaata ttccagctat gtatcccatc ctgaagataa
    14761 tcaatgtaaa agcttccaag agtatcattc tcaaatttag aagtttcaag aattctatca
    14821 ttaacaataa tactacactt aatttcttca gccatattat ctcttacata aaactcaaac
    14881 aaaacttctc tgttctctgt gaaaagtgtg ttttcaacag gagaatcaag ttcaacttca
    14941 ggttttgtta catcatctaa attagaaaca cctttagtag gatagtataa tatttcccaa
    15001 ttatctgaac catcaggtat tcttgcaaga cttacaatgg aattaaaccc actattgaaa
    15061 ttttcatatt caactctgtt tacaattata ttactacatc ttaattcaat atatccacta
    15121 ttttgcaatg cattccatcc aaatgcagca aatcctttat tgtttaatct tccatacatc
    15181 tctaattctt caatcccatt tgaaataaag caattattca tgctaaatgg aatttcttct
    15241 ggattataca attcaaccca tccttcactt cctgttacaa tttcatttat gacaattttt
    15301 tgcattacat tgactttaac tgtcttatat ccataatcct ctccatcata agctcctatt
    15361 aaaaattcat aaattccaaa ttcttcttca gttgtctccc attcaaaatt attttcatca
    15421 gatacaaatc ttgaatcatt tatttcatat gtaatttcat cgccttcaat atcacttgca
    15481 aatacactaa agcttagagt atctcctgca agcacaaatt gctcttctat atcttctatt
    15541 ataggcgcat cattaactga attaaccttt acaataaatt tcaattcaac ttgaaattct
    15601 tcatcattaa ctataatttc aacttcttct tcaccatacc agttttcttc aggaattatt
    15661 gatattgaaa ataaatcttc acttaattgg agagttatat gtgaagtgtt ctcaaaagaa
    15721 tactccaatt cactgtcagg gtcataaact aatccttcta agattaattc ataaactccg
    15781 tcttcataca tatttattac ttcaatattc ccaagaataa caggagcatc atttacaggt
    15841 aaaactctta atgttatgtt attactgtaa actacattta gcccatcagt aactttgaat
    15901 attatccaat cttctccaga ccaatctttt ttagatttaa agtgtgcaac acccccttct
    15961 atcttttcaa gtattatatt tgagttgtct gaactctctg aaaacaaata atataattta
    16021 tctccatcta aatcatagaa atactctgtt aaatctaatg tgtgagtagt atcttcatcc
    16081 cattcttgtt caggtatatc cataattaat tcaggagctt tgtttctatt ccagacttta
    16141 acactaaacc tcttgcttga agttaattct ccgtcgctgg ttaagacaaa gaaactataa
    16201 tctccttcat cttcatatcc tgtttgccaa ctaaaatgag ttgggtcttc ttcatcctgc
    16261 acaaatcttg aatcatttat ttcataggtt atttcatctt cttcagcatc atatgcgatt
    16321 atgtaaactt ctacaatctc accctcatga acagaaattt tagatattgg ctctattata
    16381 ggagcatcat taacgcaatt catataaggg tcacttgaac caggattaat caatggatta
    16441 tcatcgtcac aatcatctcc tccagcaata acagcaataa atccatctcc atctaaatca
    16501 tttaaatcac ttccatcaca atcttggtca attccatcat acggtatttc ttctgcatct
    16561 ggatttatat ttgggtcttt atcattgcaa tcttcatcac tgaaaaaacc atccccatct
    16621 ctatcttctc cacccatagc ccactcaatt gaattgtaaa ttatttcttc tgcatctgga
    16681 gtcaataaat ttgtttctgt aaacccaata aacactcctc ttgcatttga aacctgtcct
    16741 cctcttaatg cagaaccatt atttattgct gcaacaatag ccccattaaa ataatttggt
    16801 ttatctgtaa ccattacttc aagtcctggt gcaggataaa atcttgaaat atattttaat
    16861 ctgtaagaaa tcataacatt atctttataa taaggtatga aagcatcagg aacattctgc
    16921 gttattaaac tttcagaatc tctaacatat atttcacttg ggtaatttga agcttttgtt
    16981 gaaacacttc tccctgtcca tccccaggtt ccaagatgat aactgttaaa tataactgaa
    17041 tttttttgat taacaggaat aagtgaagga ttgttgaaat ttccctctcc tacaattatg
    17101 acactatact ttgaaaaatc aattaatgat aaatcattct gataaataac atcataagta
    17161 taatttgcat tattcaatat attaattatt gcaggatcag tatagcttgc acctctggtt
    17221 atgtatgcaa tatcttttgc tgcaacactt cctattgtta cgaacattaa gaatactact
    17281 acgaggctta agaggacttt gtgttttcta tttttcattc ttattttctt cttacttttc
    17341 ttttactttt cttcctcttt ttaccccaaa tgtaaattaa tattaacaat actacaaata
    17401 atattagtgt gttcattcca aatattttga ctaattttga aggatttgtc tgttctccag
    17461 gattttctgg atttgtttct tcctgactct ctttaggttc ttctttttct ttatatcctt
    17521 ggtctttctt tgcaataact ataattgttg aagataatac aactcctgct tcctttcctt
    17581 caactggttt aaagaaaaca ttaactctcc cttcataggt tccttctttt tttactttta
    17641 ttgttatctc agctttttta ctctcccctg cttctaaaat aaatgtttca tcaactaatt
    17701 caataaattt agaagcattt gaatctaatc taagagttat atttacagga atactattaa
    17761 cattgtttac aagaattgtt tttggtataa ctgtgtttgt ccaaccatta acttctggat
    17821 atagtaccat ccttccattt ccaatagagc cagtaactgc agaaatcatt gtaattcctg
    17881 tcatcaaaac tacaattgca aatgcaaaca ccagtgtttt tttatttctc atgtgacact
    17941 ttagaatgaa tacactattt aaagattgtt gtcgtcgaga aacttattat ttgttattac
    18001 ttttttttaa tgaatttatc tttttttctt ttttcttgca gattctaagt cctttttagg
    18061 gctctcttta acactattct tcatctcaaa gtaaaagttc tttaagaata atataaacat
    18121 aggtgttaca accaatgcaa ttacaaaatc ataaattaaa ttcaatgtaa gtgaacctaa
    18181 aagcaatcct aaagagatat tagatgagga aattgttttt agtaaaactg gaatacctat
    18241 tggaagttta ataattgata atattaacac aaaagccaaa gttacaagtc caaaaagtat
    18301 catgtatcca aaagttttcc accatctttt tttcactatc cttttacttt ctttaagaga
    18361 atctattatc ttctgttttt tctccaagaa aacatatgct gcaaaaaccc agtaaaccgc
    18421 aaaaataact ccaggtatta taagtaaaag aaacaaaagc ataataaata tagttactac
    18481 aatagtaaat ccaaagtatc tccagtaatt agtttttcca ttttcaagta tttctttgta
    18541 agagaattta tcttttttaa gtgaaactgc tattaaaccc acacctacaa ataaagctaa
    18601 gaaaaaataa acaatattca aactaattga aacaatatca taataaatag gtaactttat
    18661 ggttccttga ttatattgtc caatcaaaag aggattagac attatattga aaattctttc
    18721 atctgtaatc aatgtaattg aattaaacaa aaaagcaaga acaagtatta ttccaagaaa
    18781 aattaaaaca agttttaata ttggtttgaa atttctttta tattcattaa cacttttatc
    18841 aaacaaactt ccgaatgtga acttatcttc atcacctctc ttttgttttt ccatatgtaa
    18901 aaataacaaa atatgtttat ttaaatatta ctattttgat aaaactaaaa attctttctt
    18961 aattatatag cttcacccca gaagtaaatc tgtccagtat caaaatcacc attacatggt
    19021 tctggaagat taagtctgaa tatcaaagtc atttctgctc caggagaaag gatatttgaa
    19081 aaataatatc catagttatt atttgcctgt attatttcat ttctgttata gaatctgaaa
    19141 gggtcattga aacctgttcc ataatttaaa gaacagtatc cttcatcatc acaagttctt
    19201 ccaacttcca tatcttgttt tgtagagtaa gctccatttg ttgcatagta tgcgaaattg
    19261 cttaaatcta atttgtttgt agttggacat ttagctcctg agcttgatga atcataaaag
    19321 tctgttccag atataaacat atcaa
//
LOCUS       DAOPCH010000010        19145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_2971_length_19145_cov_5.719068, whole genome shotgun sequence.
ACCESSION   DAOPCH010000010 DAOPCH010000000
VERSION     DAOPCH010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 19145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19145
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2971_length_19145_cov_5.719068"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..309)
                     /locus_tag="PLU55_01285"
     CDS             complement(163..309)
                     /locus_tag="PLU55_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86724.1"
                     /translation="MKLNKKCKCGCEIIFEVDKTYKLPKTKECFKCGNKVEIVSDEKE
                     GENK"
     gene            complement(306..722)
                     /locus_tag="PLU55_01290"
     CDS             complement(306..722)
                     /locus_tag="PLU55_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86725.1"
                     /translation="MKVWIKLKLIKLFKLEHLSDVISKERDKQKDIDKKFYDNRVREI
                     IEKKDREIEYLRTSYDYDIKTLEEEIRTLKIKEKEVDEKDLRAKNQINANVHIATRMY
                     SEMKNCSDYLVKTTAKINRAMNEALDHKKVVEKIEK"
     gene            complement(806..>2036)
                     /gene="nrdD"
                     /locus_tag="PLU55_01295"
     CDS             complement(806..>2036)
                     /gene="nrdD"
                     /locus_tag="PLU55_01295"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPJ86726.1"
                     /translation="EVIMMSLGYVSIINESDRTDEPVIIRDQEFNRNYVLYCIRYYTP
                     NTKRTQKNIYKVINNSVAFKIKSIEVIKDYSKENVFCFEMDNKEEPYFTLPNGIITHN
                     CRLINNVEDLINATQNEYMNLIGGASIKVGSFGVTTLPLVRIALKAKKNEELFFKLLE
                     EEAQNCYKINHCRRYLISEKIEQGQMPLYTEGFIDLKNQYSTMGINGFYECLLYMGYD
                     ITKSEGIEFGIKILKVLDTVSKDKLKKYGYRTNIEQIPAEQTSSKLAKADKILYSQND
                     FYIYGNQFIPLTIEEGLYKRIEIQADLEKYFSGGTILHVNVNEKIPTKSLMKKIIKFV
                     IKSGVKYFAINYIMNLCENKHYTVGDYSLCPDCQGKIVDHVTRIVGFPVPVSAWSSER
                     QKEFYDRKKYKIEDLNM"
     assembly_gap    2037..2046
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2047..3796)
                     /gene="nrdD"
                     /locus_tag="PLU55_01300"
     CDS             complement(<2047..3796)
                     /gene="nrdD"
                     /locus_tag="PLU55_01300"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPJ86727.1"
                     /translation="MYIKQDYDDGFEHLMLDLKEKYPKAIFELSGISVEDLDHTKFAK
                     KYFSKKNVADASVDANANVQIKNIATFMVERHKGSDKLDSLFLFWKTAKKLFSEREAN
                     ILIEKEINKDLNVQDCGNFFLPYCIAFDTYDLLLKGLPFVTNYPSAPAKHSDVFWQHT
                     VQLLQSAAPQMMGATAIPNFIVIYSALLKYDSLNEEYPIPNYNTHKKLFERYVTQRFQ
                     ELVFTLNQPLRQVQSTFSNITIFDSFFMKELCSKYIVNDTLIDSDFAMYIQKLFLLTV
                     KELNKKQIGTFPILTAQFKKDENGEVEDTEFLDLIAEVNLDSAHLNIYSDVSLTSLSS
                     CCFDGEQKILVKSSTKGVFLTSFEDFKNLPYTGNKENLKIAHNGNWVTGKFLEIPRSN
                     KLMYKITTVNNKELLVTSDHKHLTTEGDKITSNLTESDYLVFSNRPFSAIPENNQNLT
                     YEQGVLIGAYLGDGSRRKLKDVNSASVYFSLNKDKYEKLLTLFKKALNDFGVDSHICL
                     ATPYNNVYPVQITSLKLFDILNYWVSGNYCYTKEINLNCLTQNEEFRKGIIDGIYLTD
                     GGNSNRIYTTSKNLVNQ"
     gene            complement(3816..4316)
                     /locus_tag="PLU55_01305"
     CDS             complement(3816..4316)
                     /locus_tag="PLU55_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HPJ86728.1"
                     /translation="MIQHTFIDYPHPEKDAVIYYMSGCQHNCLNCHSPELQEFERYSR
                     PIMDTIDLLSKALQKYKTNNLVISGGDCLHPYNLQLTKCLCNWFKKIQYINICLYTGF
                     DIEYIEKCGIVNFDFIKGGKYIEKLKQKSEKTDEYIQFASKNQFLIDTNKNVISKNGR
                     YVFPKN"
     gene            complement(4313..4819)
                     /locus_tag="PLU55_01310"
     CDS             complement(4313..4819)
                     /locus_tag="PLU55_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86729.1"
                     /translation="MSVGCVKIYEDKIVLGTDSIIVYGGSQEKKKDAKIKKIDENFGF
                     VSTGVVKEIEFFFIFCKTHKPSHNTIEGIIDYFFEFSIWKKKKIDDDKIENTYIIVYE
                     GKSYYFYDYDVTPINDYSAIGAGWQYATTSLYLGHDVEEAIKTACELSIYCELPSNII
                     EFKKETSK"
     gene            complement(4829..5113)
                     /locus_tag="PLU55_01315"
     CDS             complement(4829..5113)
                     /locus_tag="PLU55_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86730.1"
                     /translation="MINNRIKKKYILKCMCSSPNHKIFLTKEYSGIGFISIVKNFSEK
                     SSFWERVKSAIKYVLSEEEPMIDKEFVIRKEDVIKLENFVIDLKESDIYR"
     gene            complement(5103..5822)
                     /locus_tag="PLU55_01320"
     CDS             complement(5103..5822)
                     /locus_tag="PLU55_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HPJ86731.1"
                     /translation="MKEIKKYIEKVEDGEIVLYDNPEYIKYKVEKLYEVYLKKSGIPQ
                     YYWNINFEDYVGDKNSKAFKMLYNYANNILDKKYDFVNLYIQGSSGTQKTAIGINILK
                     SAMKQGLRAKFILAGQLINKLMKLQSYNVDDRIQEEVNLIRDADIILVDDIFDSEKSL
                     YWKGESNSLIISEWDTFFRDMIAQGKKIILTSNFSLEIIQQKYGDYLYELIHRNFKEI
                     ELRDSVKEKRKLTVEGAFDDK"
     gene            complement(5809..6255)
                     /locus_tag="PLU55_01325"
     CDS             complement(5809..6255)
                     /locus_tag="PLU55_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86732.1"
                     /translation="MKDHRFLGHILNYDCEKDIITIKVDFLDMEKQEIIEQIAKEKTI
                     FSFCFSRPFRKSKAYYQLKYYFKLLKQIVIGFGVTPSSEVIKSLDNEHKKRCFPSQNI
                     DLGNQVIPVVKSKADMTFEEMKYLTDELKDTYSEILIQEEFEGEGN"
     gene            complement(6316..7290)
                     /locus_tag="PLU55_01330"
     CDS             complement(6316..7290)
                     /locus_tag="PLU55_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86733.1"
                     /translation="MIERFKNKSVYGVFDHDLDGNTSLIVYKSIVEPHVKSSRVTSTG
                     NREMEDFSFDYMNESDIIIFVDIAPTLSLYNKIKELNKEVYIFDHHETSFNILKDIVT
                     ENYYYSEVFCGAKVFFDALTKGKRYNKVLSQMVELCNVYDTWQKTSLLWKTAKDLNNV
                     MFGYVNWATDKDNPKKFDKFIEEQLNKIKTGRFFYFTSYEEDKIKLAIKKEQDALRQA
                     RTCLKHRIDNQGNNYIYLEINSKISYVSNQLLEEMEKVKYVIGHSTYEDSNGIKPQIS
                     LRTINDRNIDVSKIAERWGGGGHPCSSGVLFKDLADFIRLQKGLIHLI"
     gene            complement(7320..8804)
                     /locus_tag="PLU55_01335"
     CDS             complement(7320..8804)
                     /locus_tag="PLU55_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPJ86734.1"
                     /translation="MKNIKEGFLKDSILTVIEKTCRILSRKVGTIISVSPIPTDVISG
                     SRKLSGVDCYFLDGRKIRWNWYSNDTSSNIVSVSYWTKMKIQPDIEIDLHDYNIIQSI
                     NAVAEVINNNKLGTVVLEESVSKYNEAEISDAIKQAIKGWSEHMKIDDDKLTNTRMKD
                     LWRSFRFWYEEEGGAQQGFRDMSEASFRNYMLAYFDKYGIKNIYVKVIKSGTASKEKL
                     AFDSIKAKKFNAEIYKMTMDEQREFILNSLYMLAQGLVNSLIIGGQAGAGKTHIVESA
                     LGRVKGMNIVYLKGGMKNTEELFKFLQSNNSEKTMIVFDDFDFVFDKKNLDIMKAVMS
                     PDKKRTVTWYSGKINKDNIYDVSDDLDVAIATQKGIDIKKSNKKSYVPTFEFKSKILI
                     ITNRPKAKVDSALVSRGTYIEVNFTKEDIIADIRKNLFVLFPEYKDKVTDAMKLEVID
                     FLSEYISNIDFIDYRIFRTALGIRAVDPYNPLWKKFVIMALSVL"
     gene            complement(8868..9146)
                     /locus_tag="PLU55_01340"
     CDS             complement(8868..9146)
                     /locus_tag="PLU55_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86735.1"
                     /translation="MSITKTWYPQKGVRYTVNAEEAGSGLVAIPYETAPTKNLLSVVQ
                     VSTSSGVTKAGFKKTYNSGLGTLTIENSAGSFTFAENDIIELLGCWYV"
     gene            complement(9219..9341)
                     /locus_tag="PLU55_01345"
     CDS             complement(9219..9341)
                     /locus_tag="PLU55_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86736.1"
                     /translation="MLKFVNLNKEKYCKDEFEIKTLLKFKGYALQLVKSKVKQN"
     gene            complement(9343..9765)
                     /locus_tag="PLU55_01350"
     CDS             complement(9343..9765)
                     /locus_tag="PLU55_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86737.1"
                     /translation="MELKENVKLFDEKRKAIETIDSEIASIQEKYNAEIAELKSKKDV
                     IAKEMGDTFSLIESEVKAEFEKNPKVKKFFGGFGVQERKKYIYDEKLAFAFAKEKDMF
                     LQLDVKAFEKAIEGLNLDFVKEDKEVKVTTPKVIVFED"
     gene            complement(9781..10473)
                     /locus_tag="PLU55_01355"
     CDS             complement(9781..10473)
                     /locus_tag="PLU55_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86738.1"
                     /translation="MKLIIRTHVKNEERLQLLKQSIVSAQSQDFKYIVIVDDQSPLKE
                     KVKEMVKSLNVSDYVLTEGKPDTKNGFYYSLKQMTDSPAILAVDDFVCSKYLRKEFDC
                     VAEDIKEIKDIYGCIGFFACYPSEIRKRYGNTNLWNINIDALYALLFHIISPKLKEIM
                     IKEWESVLKGEFPYPEMCDDLWLATVCKREGLLAFNTLMDYIDHTGIKQRSFGDVVED
                     DNSCYTSPAFVK"
     gene            complement(10476..11129)
                     /locus_tag="PLU55_01360"
     CDS             complement(10476..11129)
                     /locus_tag="PLU55_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86739.1"
                     /translation="MLNLINIIKQNRFVVITGQAGAGKTYLASKCKEQGFANVYSTDF
                     RFFGDSDYRKTLLETKKNNFFDYVEAMNQYSWWDWNIIEKEVLYHLKDKSKFVLETIY
                     NRDTGNIIDILEEDKKETLILEGAIISESLLKKCDKIVCVVADDELRFNRLLKKDRTR
                     RHFIEIINRYMITNYSENLYFNNVLSLFKDKIIFTDENYSIINYKFTNRKNYLPLEV"
     gene            complement(11113..11568)
                     /locus_tag="PLU55_01365"
     CDS             complement(11113..11568)
                     /locus_tag="PLU55_01365"
                     /inference="COORDINATES: protein motif:HMM:NF037735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD domain-containing protein"
                     /protein_id="HPJ86740.1"
                     /translation="MKKLLNLVQKRIFKQQESFGKKQFDKSKTIFCDMDGVICYWKKD
                     NYNKDEVLIMENVNFLKKLINEKNVKIVLTTARSKKKCKQVLKLLKHQGLNFYKCVYD
                     LGVGQRLVINDKKSTGLKSAFSLNTERNKSFFDLYKELIEGSDFDVKLD"
     gene            complement(11495..12232)
                     /locus_tag="PLU55_01370"
     CDS             complement(11495..12232)
                     /locus_tag="PLU55_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPJ86741.1"
                     /translation="MLGQVFSLYGDKYNVVILAGGAGTRMGEQSDYICKALTPLGERR
                     AIDFIIERYNNVAHKFIIGTGYHADLLKSYILGNYNQNKFVFSHEKPNEIKNNAYSFM
                     YCLDYCDVRYPTIVLFCDLLLLGNFNIEKNSFMLATTKTKGQIGTFRHTYNFSENWFC
                     YNENNPVKPTEEDLGVMGNFVFGDTYHLKKITYSTPFKDLKDITNDVVIPYHKDIYMK
                     GVCCEKVIEFGTEADIQTARELWEKTV"
     gene            complement(12237..12863)
                     /locus_tag="PLU55_01375"
     CDS             complement(12237..12863)
                     /locus_tag="PLU55_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPJ86742.1"
                     /translation="MPYCDHNYLKYYKPKKEDIVLDLGASLGEFENDNLSLLKEKNCK
                     IICVEPTLWCLDKLANYMSENFALDKFIILSLGTSKENTCLSFENTSSHVLNCISSLD
                     STKNKYDVTSKVSKVPVISLDTLLDITGEIDFCKCDIEGGEIDTFLYSKNIRKIKNLA
                     IASYHVVNGEETYIKLKSFFEKNGFEVKYEKEKYLDFEPQSMLYCTRR"
     gene            complement(12863..13891)
                     /locus_tag="PLU55_01380"
     CDS             complement(12863..13891)
                     /locus_tag="PLU55_01380"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPJ86743.1"
                     /translation="MTKSILILGGYGLIGQEIAKRYYNEGWNTYIWDNKSNPYIDYTD
                     DKLVGYNIFDNYDGSLFALLDNVKFDVISNQASLVSVGESQFQIQKYMDNNIGIVSEL
                     MQAMIDTKNFPEFLFHASSMGIYGDTLFSDDYTLLEESIKIPSSFYGISKYCQEEMFR
                     VFSDLYNVKTIALRYFSVYGNINPINPLTGIISVIVNKLLNSDVVELNDDGNQTRDFV
                     HAEDISNLHYSLMNKPEVISSNFNFFNVCTGESHSLKEIAYYIKGVLQSDKEIIFNNK
                     SRKGDIYYSRGSSTLSIIYPEFKYNKNLYEEIDKYIKYVLKNKDKFIVGYDSCKEADE
                     KLKEKGLF"
     gene            complement(13888..14919)
                     /locus_tag="PLU55_01385"
     CDS             complement(13888..14919)
                     /locus_tag="PLU55_01385"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPJ86744.1"
                     /translation="MENKKVALVTGSRGMDASNLIKFLLNKNYTVIGTDRYSSSPDFW
                     RHEELGIKGKFTHVIMDITDFSNVSNVVKTYQPDEIYNLCAMSFVAESFNTPLSTFQI
                     NTNGHLNLLEAVRQFSPQSKIYFASTSEIFGKVQEVPQRETTPFYPRSPYAVAKLSSF
                     WLTKNYREAYGIFSCSGVLFNHSGILRGESFVTRKITKNIAKVLTGKIDFFELGNINS
                     KRDWGSSEDYIEAMWLMLQRETPEDYVISSGETRTIREFIQECFDVLNIKIEFKGEGL
                     NEEVYIVGSKTPIIKISAKYFRPAEVNLLIGDCSKAEKELGWKRKISFKELVKSMMLF
                     DAKKEGYIV"
     gene            complement(14919..16040)
                     /locus_tag="PLU55_01390"
     CDS             complement(14919..16040)
                     /locus_tag="PLU55_01390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPJ86745.1"
                     /translation="MKKILLVAPVGKDNAINGGYNKIANNLDYIFKLKKHEYNYNYTS
                     CHVSEFENIDEEFDIVLFQCHPNMFLFDKKFLSIYKSKKDLFKKTYLHIAWETSPFPN
                     YWKVLNNFFDGYATTSDFCKTEMKSLTQKICYKIPFYIEDSFYSLYKGRLSLTDKKEE
                     KQFTVLFMGQNTKRKGIEDALTAFSIFSNGKEDDVRMICKYHNLSDKEIPVPVLAKNI
                     IETNSYTLNRKPNIFLLDYDIDDKTIIQLYKDSSLLLQCSRGEGFGLCGLESNLVGIP
                     HTYTNFSSTKEVGEYCKSINAPIDCDLVPSYGMSQYHYKINSNYGLPKLNSILYNLEM
                     YYGAWKANKMKYYEDALKGTEKLPEYLNGMKNEFLEGVL"
     gene            complement(16040..17695)
                     /locus_tag="PLU55_01395"
     CDS             complement(16040..17695)
                     /locus_tag="PLU55_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86746.1"
                     /translation="MNYNKDVFEILPTLKDNSISFVFSDPPYNMGSKYRINPETGKPQ
                     EVILSEFMGDKWNGLTCEQLEIFFKECFRICKYGAYVVLYGIDRVQMPFEYFAQLAGF
                     EPQQYLYSYKIKSFPKASDVGKLLDKREGAEREIIGKRTGRASKPIMDIRNNNFAKGG
                     SGRIDCSDITAPSCNLAKKYDGYKVSISPFKQVLETIMIFKKPYPKGMSNIDAIYKYE
                     NGNKEIHPSLINIDGGRVLTEENLQRECLGLASSANEGYKRPFHEFAIKQNYGSTSGR
                     YPAQLLIVDDERLKEEIGYNTSDIIDNQSGIQKSSDSIRHNNQEEYAEKDYRKYGKYK
                     NSDSSGFNDIGGASRILHKCNFTEEEYILQDCLYEQGNFDILTYCTKVSNAERNAGCE
                     ELNNKKRYGQGIYSQSPICKTCNKTINGTNNHDGCSGEIYYNIKEENLTQKNNHPTLK
                     NLELNKKITQLFILPKENLRDFTVFNPFSGTRSERIGLLSNGVLEENIISCELNSDYF
                     LIGEKREKYWKEHNFKFMKEERKELEKVKKENKTKCENEEGWF"
     gene            complement(17697..18992)
                     /locus_tag="PLU55_01400"
     CDS             complement(17697..18992)
                     /locus_tag="PLU55_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86747.1"
                     /translation="MKILFCSDSLNHVTGMGHVILNIIAGLNKEYKDLEIVYCTFTGQ
                     PSKKEDYIFHGEQYKNLFDKMDFEFISPMDANKQDIFDRVVEHYKPDLVFSFVDAWLL
                     EPIAYSDYRENYKWITYLTFEVNDYPVWCYFPCFKFKQARINIRELLAKADMNIPVAK
                     IGENNLSNFGVKFCENIYLGLDIEILNSIKDKDKLKKSDVFGEAVRDDDFLFMTLGKN
                     TERKRIDFVIDAFHKFLMCKEEQDREFYKLYVHSNINETYDSGTDLLTMISNLGITKN
                     IVVPDCYRKNEDLPKEELYKRYLVSDCYVSLSVGEGFGLGAIDALLCGKPLIYSRETV
                     IEEIGKRCGLPVNTIESVSARNNFIKFKYPSIRLASTMMQSMEDIIKSNSGIYRDFLE
                     RCNKATKDLTWENCVNKIKVQIDEVMKNSKKVKYFLRKV"
BASE COUNT         6350 a         3286 c         1954 g         7545 t
ORIGIN      
        1 tttcttttgt aaagcactta tttaaatccc aatctataat atcaaaaaca tctaaaaaag
       61 tccccaattc tttattgact tttctcatta catattgttg gtaataactt atagtatcat
      121 caaagtctac tgatatgttc aatgactttc ttaaagcatt actcatttat tttctccttc
      181 tttttcatca ctcactattt caaccttatt tccacacttg aaacattctt ttgtttttgg
      241 aagtttgtat gttttgtcca cctcaaaaat aatctcacac ccacacttgc actttttgtt
      301 taatttcatt tctcaatctt ctccactact tttttatggt caagtgcttc attcattgcc
      361 ctatttattt tagctgttgt tttaactaaa taatcagaac aatttttcat ctctgaatac
      421 attcttgttg ctatatgaac attagcattg atttgatttt tagctctcaa atctttttca
      481 tcaacttctt tttcttttat tttaagagtt cttatttctt cttctaatgt tttaatatca
      541 taatcataag aagttcttaa atattctatt tctcggtctt ttttttctat aatttctctt
      601 actcggttat catagaattt tttatctatg tctttttgtt tatcacgttc ttttgatata
      661 acatcgctta ggtgttctaa cttaaataat ttaattaatt ttaattttat ccagactttc
      721 aatttctttc tcctttttta aacttttaat aatttactaa aaatcagtca agactttttt
      781 tgaatcctga ctgatttttt tataattaca tattcaaatc ctctatttta tacttctttc
      841 tatcataaaa ttctttttgt ctttctgatg accaagccga aacaggaaca ggaaatccca
      901 caattcttgt aacatggtca acaatctttc cttgacaatc tggacaaaga gaataatccc
      961 ctactgtata atgtttattt tcacatagat tcattatata gttaatagca aagtatttaa
     1021 cacctgactt aataacaaac ttaattattt ttttcatcaa gctttttgtt ggtatctttt
     1081 cgttaacatt tacatgaaga atagttccac ctgaaaaata tttttccaaa tctgcttgaa
     1141 tttctattct tttatataat ccttcctcta ttgttaaagg aataaactga ttaccataaa
     1201 tataaaaatc attttgacta tatagaattt tatctgcttt tgctaattta gatgatgttt
     1261 gctccgcagg tatctgttct atatttgttc tataaccata ttttttaagt ttatctttag
     1321 aaacagtatc taaaacttta agaattttaa taccaaattc aataccttca gattttgtta
     1381 tatcataccc catataaagt aagcactcat aaaaaccatt aattcccata gtactatatt
     1441 ggttttttaa gtcaataaac ccttctgtat ataaaggcat ttgtccttgc tctatttttt
     1501 cacttatcaa atatcttcta caatgattta tcttataaca attttgtgct tcctcttcca
     1561 aaagtttaaa aaacaattct tcattttttt tagccttcaa tgcaattctt acaagaggaa
     1621 gagtagttac cccaaaagaa cctactttta tagaagcacc accaatgaga ttcatatact
     1681 cattttgtgt tgcatttatt aaatcttcta cattgttaat caatcgacaa ttatgtgtaa
     1741 ttattccatt aggaagtgta aaatagggtt cttctttatt atccatttca aaacaaaaca
     1801 cattttcttt tgaataatcc ttaataactt ctattgattt tattttaaat gctacagaat
     1861 tatttattac tttataaata ttcttttgtg ttcttttagt gttcggagta taatatctaa
     1921 tgcaatataa aacataattt ctattaaatt cctgatccct aataataaca ggttcatctg
     1981 ttctatcaga ttcatttatt attgaaacat aacctaaaga catcattata acttcannnn
     2041 nnnnnngctg atttactaaa tttttagaag tagtataaat tctattagaa ttaccaccat
     2101 ctgttaaata aataccgtct ataatcccct ttctaaattc ttcattttgt gttaaacaat
     2161 ttaaatttat ttccttagta taacaataat ttcctgatac ccaataattt aaaatatcaa
     2221 aaagttttaa agatgttatc tgaacaggat aaacattgtt atatggtgta gccaaacaaa
     2281 tatgtgagtc caccccaaaa tcattcaagg cttttttaaa caaagtaaga agtttttcat
     2341 acttatcttt atttaacgaa aagtaaacac ttgccgaatt aacatccttt aactttcttc
     2401 tactaccatc ccccaaataa gccccaatca gcacaccctg ttcataagtt aaattctgat
     2461 tattttcagg aatagcagaa aaaggtctgt tagaaaaaac aagatagtca ctttctgtaa
     2521 gatttgaagt tattttgtca ccttccgttg ttaagtgctt atgatccgag gtaaccaaaa
     2581 gttctttatt attgactgtt gtaattttat acattaattt attacttctt ggtatttcca
     2641 agaatttacc tgtcacccaa ttgccattgt gtgctatttt taaattttct ttatttcctg
     2701 tatatgggag atttttaaaa tcttcaaaag aagtaagaaa cacacccttt gttgaacttt
     2761 ttacaagtat cttttgttca ccatcaaaac agcaggaact taacgaggtc aaacttacat
     2821 cagagtatat gttcaaatga gcactatcta aatttacttc tgcaattaaa tcaagaaatt
     2881 cagtatcttc aacttcacca ttttcatctt ttttaaattg tgctgttaaa ataggaaagg
     2941 tacctatctg ttttttattc aattctttta cagtaagcaa aaataatttt tgaatataca
     3001 ttgcaaaatc agaatcaatt aaggtgtcat taactatata tttagaacac aattctttca
     3061 taaaaaaaga atcaaatata gttatatttg aaaatgtact ttgaacttgt ctcaaaggtt
     3121 gattaagagt gaacaccaat tcttgaaatc gctgtgttac atatctttca aataattttt
     3181 tatgtgtatt ataatttggt attgggtatt cttcattgag agaatcatat tttagtaatg
     3241 cagaataaat tacaataaaa ttaggaatag cagtagcccc catcatctga ggagcggctg
     3301 attgtagaag ctgtactgtg tgttgccaaa acacatctga atgctttgca ggagcacttg
     3361 gataatttgt aacgaatgga agtcctttca ataataaatc ataagtatca aaagctatac
     3421 aataaggaag aaagaaattc ccacaatctt gaacatttaa gtctttattt atttcttttt
     3481 caatcagaat atttgcttct ctttcactaa aaagtttttt ggctgttttc caaaaaagga
     3541 aaagagagtc aagtttatct gaacccttat gtctttctac cataaatgtt gctatatttt
     3601 taatctgaac attagcattt gcatctactg aggcatccgc tacattcttt tttgagaaat
     3661 attttttagc gaattttgta tggtctaaat cttctacact tataccacta agttcaaaaa
     3721 ttgctttagg atatttttct tttaagtcaa gcatcaaatg ttcaaaacca tcatcataat
     3781 cctgtttaat atacattaac caccctcttt attatttaat tttttggaaa aacatatctg
     3841 ccatttttag agatgacatt tttattagta tcaattaaaa actgattctt agaagcgaac
     3901 tgaatatatt catcagtctt ttcagacttt tgctttaatt tttcaatgta ttttccacct
     3961 tttataaaat caaaattaac tataccacat ttttctatat attcaatgtc gaatcctgta
     4021 taaaggcaaa tattaatata ctgtattttt ttaaaccaat tacataagca ttttgttaat
     4081 tgaaggttat aagggtgtaa gcagtcacca cccgaaataa ctaaattgtt agttttatat
     4141 ttttgaagtg ctttacttag taagtctata gtatccatta taggtctact atatcgttca
     4201 aactcctgta attcaggaga atgacaattt aagcaattat gttggcaacc tgacatataa
     4261 tatattactg cgtctttttc agggtgtgga taatcaataa aagtatgttg tatcattttg
     4321 atgtttcctt tttaaattca ataatgtttg aagggagttc acaatatata gaaagttcac
     4381 aagctgtttt tatagcttct tctacatcat gtcctaaata aagggaggtt gtagcatatt
     4441 gccatcctgc cccaatagca ctataatcat ttataggagt aacgtcataa tcataaaaat
     4501 aataactttt accttcataa acaattatgt atgtattttc tattttatca tcatctattt
     4561 ttttcttttt ccaaatagaa aattcaaaaa aataatcaat gataccttct atagtattat
     4621 gagaaggttt gtgtgtttta caaaaaataa agaaaaattc aatttctttt acaacacctg
     4681 ttgaaacaaa accaaagttt tcatctattt tcttaatttt tgcatccttt ttcttttctt
     4741 gtgaaccacc ataaactata atactatcag tacctaaaac aattttatcc tcataaatct
     4801 taacacatcc tacactcata tatcctcctt atctataaat atcagattct ttcaagtcta
     4861 tcacaaaatt ttcaagttta attacatctt ctttcctaat tacaaattct ttatctatca
     4921 taggttcttc ttcggaaaga acatatttaa ttgccgattt tactctttcc caaaatgaag
     4981 atttttctga aaagttttta actatactta taaaacctat accagaatat tcctttgtta
     5041 aaaagatttt atggttagga gatgaacaca tacactttaa aatatacttt tttttaattc
     5101 gattatttat catcaaacgc cccttctact gttagttttc ttttttcttt tacactatct
     5161 cttagttcaa tttctttaaa atttctatga attaattcat aaagataatc cccatatttc
     5221 tgttgaatta tttctaaact aaaattactt gttaatatta ttttttttcc ttgtgcaatc
     5281 atatcacgga aaaacgtgtc ccactcactt ataataagac tattactttc acctttccaa
     5341 tatagacttt tttctgaatc aaatatatca tctacaagta taatatctgc atctcttata
     5401 agatttactt cttcttggat tctgtcatct acattatatg attgtaattt cattaattta
     5461 ttaatcaatt gtcctgcaag aataaattta gctcttagtc cttgcttcat agcagatttt
     5521 agaatattta tcccaattgc agttttctgc gttcccgaag aaccctgaat gtataaattt
     5581 acaaaatcat attttttatc taaaatgtta tttgcataat tatataacat tttgaaagct
     5641 ttagaatttt tatcccctac ataatcttca aaattgatat tccaatagta ttgagggatt
     5701 ccactttttt tcaaatatac ttcataaagc ttttctactt tatatttaat gtattcagga
     5761 ttatcataaa gtactatttc accatcttct actttttcaa tatacttttt aatttccttc
     5821 accttcaaac tcctcttgta tcaaaatttc cgaataagtg tctttaagct catcagtcaa
     5881 gtatttcatt tcttcaaatg tcatatccgc tttagactta accactggaa ttacttgatt
     5941 acctaaatca atattctgtg acggaaaaca tcgcttttta tgctcattgt caagtgactt
     6001 aattacttcg gaagaaggcg ttacaccaaa accgattaca atttgtttta gtaatttgaa
     6061 ataatatttt aattgatagt atgcttttga ttttctaaaa ggtcttgaaa aacaaaaaga
     6121 aaatatagtt ttctcttttg ctatttgctc tataatttcc tgtttctcca tatcaagaaa
     6181 atctacttta atagttataa tgtctttttc acagtcataa tttaatatat gacctaaaaa
     6241 tctatgatct ttcacttgtc tttctccttt cttaattcac tctaaaatat taaagtacat
     6301 tgtcaagata ttttttcaaa tcaaatggat taaccctttc tgtaatctga taaaatcagc
     6361 aaggtcttta aacaatacac ccgaactgca aggatgtcca ccaccacccc atctttcagc
     6421 tatttttgat acatcaatat ttctatcatt tatagttctt aaagatattt gtggttttat
     6481 tccattacta tcttcataag ttgaatgacc tattacatat tttacttttt ccatttcttc
     6541 taaaagttga ttactcacat aactaatttt ggaatttatt tctaagtata tgtaattatt
     6601 tccttgatta tctattctat gttttaaaca tgtccttgct tgtctaagag catcctgttc
     6661 tttttttatg gctaatttaa ttttatcctc ttcataagaa gtaaaataaa agaatcttcc
     6721 agttttaatt ttattaagtt gttcttcaat aaatttatca aacttttttg gattatcttt
     6781 gtctgtagcc caattaacat agccaaacat tacattattc aaatcttttg cagttttcca
     6841 taatagagaa gttttttgcc atgtatcata aacattacag agttctacca tttgagaaag
     6901 gactttatta tatcttttcc cttttgttaa agcgtcaaaa aatacttttg caccacaaaa
     6961 tacttcggaa taataataat tttctgttac aatatctttt aaaatattaa aagaagtttc
     7021 gtgatggtcg aatatatata cttctttgtt aagttctttt attttattat atagacttag
     7081 tgttggtgct atatcaacaa aaattattat atcactttca ttcatgtaat caaaagaaaa
     7141 atcttccatt tccctattgc ctgtggaagt tactctggaa gattttacat gaggctctac
     7201 aatactttta tatacaatta aagaagtgtt tccatctaaa tcatgatcaa aaacaccata
     7261 tacactttta tttttaaatc tctctatcaa acatagccat cctttttctt ttgttagtgt
     7321 taaagtacac ttagtgccat tatcacaaac tttttccaaa gtggattgta agggtcaact
     7381 gctctaatac ccaatgctgt tctaaaaatc cggtagtcta taaaatcaat atttgatatg
     7441 tattctgaaa gaaagtcaat aacttcaagc ttcattgcat cggtaacttt gtctttatat
     7501 tcaggaaaca atacaaaaag attctttctt atatctgcaa taatatcttc tttagtaaaa
     7561 ttgacttcaa tataagttcc tcttgataca agagcactat caactttagc tttaggtcta
     7621 tttgtaataa taagtatttt tgacttaaat tcaaaagtag gtacataaga tttcttattt
     7681 gacttcttaa tatcaattcc tttttgtgtt gcaattgcta catccaaatc atccgaaaca
     7741 tcataaatat tatctttatt tattttgcct gaataccaag tgactgttct tttcttatct
     7801 ggtgacatta ctgctttcat aatatcaaga ttctttttgt caaacacaaa gtcaaaatca
     7861 tcaaaaacta tcattgtttt ttcggaatta tttgactgaa ggaatttaaa tagttcttca
     7921 gtatttttca ttccaccttt caaataaaca atattcattc ccttaactct acctaaagca
     7981 gattcgacta tatgtgtttt gcctgctcct gcctgaccac caataataag ggaattgacc
     8041 aaaccttgtg caagcatata aagtgaattt aatataaact ctctctgttc atccattgtc
     8101 attttataaa tttcagcatt gaattttttt gctttaatgg aatcaaatgc taatttttct
     8161 ttacttgctg ttcctgattt tattactttt acataaatat tttttatacc atacttatca
     8221 aaataagcaa gcatataatt tctgaaagaa gcttctgaca tatcacgaaa tccttgttgt
     8281 gctccacctt cttcttcata ccaaaatcta aatgaacgcc ataaatcttt cattcttgtg
     8341 ttggtaagtt tatcatcatc aatcttcatg tgttctgacc aacctttaat agcctgtttg
     8401 attgcatcag atatttctgc ttcattatat ttacttactg attcttccaa aacaactgta
     8461 cctaatttat tattgtttat tacttctgct accgcattaa tagactgtat aatattataa
     8521 tcatgcaaat caatttctat atctggttgt attttcattt tagtccaata agacactgaa
     8581 acaatattag aagaggtatc attagaatac caattccatc taatttttct accatctaaa
     8641 aaataacaat caacaccaga aagttttcta cttccagaaa taacatctgt tggaatagga
     8701 cttacactta taatagttcc aacttttcgt gacagtattc tacaagtttt ttcaattact
     8761 gtgagaatac tatcttttaa aaacccctct ttaatatttt tcatatatat ttattttcct
     8821 caaaaattaa tttaaaaaca aaaccccctt tttatcgggg gcattcttca cacgtaccag
     8881 caaccaagaa gttcaataat atcattttca gcaaatgtaa aactaccagc tgaattttca
     8941 atagtgagtg taccaagtcc tgaattataa gttttcttaa atcctgcttt agttacacct
     9001 gaacttgttg acacctgaac tacagaaagt agattcttag ttggtgctgt ttcataagga
     9061 atagcaacaa gaccacttcc tgcttcttct gcgtttacgg tgtatcttac acctttctgt
     9121 ggataccaag ttttagttat agacatttaa cctccatgcc ttattctttt taacaagaat
     9181 aataacttgg tttattagtg tcttttttta actaaaactt aattttgttt tactttactc
     9241 ttaaccagtt gaagagcata ccccttgaac tttaaaagag ttttaatttc aaattcatcc
     9301 ttacaatatt tttctttatt taaattaaca aattttaaca ttttaatcct caaaaactat
     9361 gacttttggt gttgttacct taacttcttt atcttcttta acaaaatcaa gattcaatcc
     9421 ttcaatagct ttctcaaatg cttttacatc caattgcaaa aacatgtctt tttctttagc
     9481 aaaagcaaaa gcaagttttt catcgtaaat gtacttcttt ctttcctgaa caccaaaacc
     9541 accaaagaat tttttaactt taggattttt ttcaaactct gctttaactt cagattcaat
     9601 caaagaaaaa gtatcaccca tttcttttgc aattacatct ttttttgatt taagttcagc
     9661 aatttcagca ttatattttt cctgaatact tgcaatttca ctatcaatag tttcaattgc
     9721 ttttctcttt tcatcaaaca attttacgtt ttctttcaat tccattttat tctccttttt
     9781 tcatttcaca aatgctggtg aggtataaca agaattatca tcttccacta catcaccaaa
     9841 agaacgctgt tttatacctg tatggtcaat atagtccatc aaagtattaa aagccaacaa
     9901 tccttctctt ttacaaactg ttgccaacca caaatcatca cacatttcag gatatggaaa
     9961 ttcccctttt aatactgatt cccattcttt aatcataatt tctttaagtt taggggaaat
    10021 tatatgaaat aataaagcat ataaagcatc aatattaata ttccacaagt ttgtatttcc
    10081 atatcttttt cttatctctg aggggtaaca agcaaaaaaa ccaatacaac cataaatatc
    10141 tttaatctct ttaatatctt cagcaacaca atcaaattct tttcttagat atttactgca
    10201 aacaaaatca tccactgcaa gaatagccgg tgaatcagtc atctgtttta aagaataata
    10261 aaatccattt tttgtatctg gttttccttc agtcaataca taatcagaaa catttaagga
    10321 ttttaccatt tctttaacct tttcttttaa aggtgattgg tcatctacaa taacaatata
    10381 cttaaaatcc tgactttgtg ctgacactat actctgtttt aaaagctgaa gtctttcttc
    10441 atttttaaca tgtgttctaa ttataagttt caattttaaa cctctagtgg taaataattt
    10501 tttctatttg taaatttata attaataata ctatagtttt catcagtaaa aattatttta
    10561 tctttgaata atgatagtac attattaaaa tataaattct ctgaataatt tgtaatcata
    10621 tatctgttta ttatttcaat aaaatgtcgg cgtgttctat ctttttttag aagtctgtta
    10681 aaccttaatt catcatcagc aacaacacat acaattttat cacatttttt taaaagtgat
    10741 tcagatataa ttgcaccttc taaaattaaa gtttcctttt tatcttcttc taaaatatct
    10801 attatatttc cagtatctct gttgtatata gtttccaata caaatttaga tttatccttt
    10861 aaatgataaa ggacttcttt ttctattatg ttccaatccc accaagaata ttgattcata
    10921 gcttcaacat aatcaaaaaa attatttttt ttagtttcca ataaagtttt tctataatca
    10981 gaatccccaa agaacctaaa gtcagttgaa tacacattag caaatccttg ttccttacat
    11041 ttagaagcta aataagtttt tcctgctccg gcttgtcctg ttataacaac aaatctattt
    11101 tgttttatta tgttaatcaa gtttaacatc aaaatcactt ccttcaatca attctttata
    11161 taaatcaaaa aaacttttat ttctttctgt attcaaagaa aaagcagatt taagtcctgt
    11221 tgatttttta tcattaatca ctaatctttg acctactcct aaatcataaa cacatttata
    11281 aaaattcaaa ccctgatgct ttaataattt taaaacttgt ttacactttt tcttgcttct
    11341 tgcagttgtc aagacaattt taacattctt ttcattaatt aactttttaa gaaaattaac
    11401 attttccatt attaaaactt catctttgtt atagttatct tttttccaat agcaaataac
    11461 accatccata tcacaaaaaa tagttttaga tttatcaaac tgttttttcc caaagctctc
    11521 ttgctgtttg aatatccgct tctgtaccaa attcaataac tttttcacaa caaactcctt
    11581 tcatataaat atctttatga tatggtatta caacatcatt agtaatatct ttcaaatctt
    11641 taaatggggt gctatatgta atttttttta aatgataagt atcaccaaaa acaaaattac
    11701 ccataacgcc taaatcttct tccgttggtt ttactggatt attttcatta tagcaaaacc
    11761 aattttctga aaaattataa gtatgtctaa atgtacctat ttgtcctttt gtttttgttg
    11821 ttgcaagcat aaaagaattt ttttcaatat taaaattacc aagcaaaagt aaatcacaaa
    11881 acaaaacaat tgtaggatat ctaacatcac aataatctaa gcaatacata aaggaataag
    11941 cattgttttt tatttcattt ggtttttcat gtgagaatac aaatttattt tgattataat
    12001 ttcctaaaat ataagattta agtaaatctg catgataccc tgttcctata ataaatttat
    12061 gtgctacatt attatatctt tctatgataa aatctattgc tcttctttct cctaaagggg
    12121 ttaaagcttt acaaatataa tcactttgtt cccccattct tgttcctgca ccaccggcaa
    12181 gaataacaac attatattta tcaccataaa gactaaaaac ttgaccaagc atattattat
    12241 ctccttgtac aatacaacat tgattgtggt tcaaaatcta aatacttttc tttttcatat
    12301 ttaacttcaa aaccattttt ttcaaaaaac gatttaagtt taatataagt ttcttcccca
    12361 ttaactacat gataagaagc aattgctaaa tttttaattt ttcttatatt ttttgaatat
    12421 aagaaagtat caatttcacc accttcaata tcacatttac aaaaatcaat ttcacctgta
    12481 atatctaata aagtgtctaa actaataaca gggacttttg atactttaga agttacatca
    12541 tatttatttt ttgttgaatc caaagaacta atacaattta aaacatgaga tgaagtattc
    12601 tcaaaagata aacaagtatt ttcttttgat gttcctaaag ataaaataat aaatttatca
    12661 agggcaaaat tctctgacat ataatttgct aatttatcta aacaccataa agtaggttca
    12721 acacaaatta ttttgcaatt tttttccttt aataaagaaa gattatcatt ctcaaattca
    12781 cctaaagaag cacccaaatc aagaacaata tcttcttttt ttggtttata atattttaaa
    12841 taattatggt cacagtaagg catcagaata atcctttttc ttttaatttt tcatcggctt
    12901 ctttacaaga atcatagcct acaataaatt tatccttatt ttttaaaaca tatttaatgt
    12961 acttatctat ttcttcatat agattcttat tgtacttaaa ttcagggtat attatagaaa
    13021 gagtacttga ccctcttgaa taataaatat caccttttcg ggatttattg ttaaaaataa
    13081 tttctttatc actctgcaat acacctttta tataataagc tatctctttc aaagaatggc
    13141 tttcaccagt gcacacattg aaaaaattaa agttacttga aattacttcc ggtttgttca
    13201 tcaaagaata atgaaggtta gatatatcct cagcatgaac aaaatctctt gtttgattac
    13261 cgtcatcatt taattcaaca acatcgctat ttaaaagctt attcacaata acagatataa
    13321 ttccagttaa aggattaatt ggatttatat ttccatacac tgaaaagtat cttaaagcta
    13381 ttgtttttac attgtacaaa tctgaaaaaa ctctgaacat ttcttcctga caatatttac
    13441 ttattccata aaaggatgaa gggattttaa tactttcttc caataaagta taatcatccg
    13501 aaaataaggt gtcaccataa attcccatac tacttgcatg aaataaaaat tcaggaaaat
    13561 ttttagtatc tatcattgct tgcataagct ctgaaactat tcctatgtta ttatccatat
    13621 atttttgaat ttgaaattgt gattcaccca cacttactaa tgaagcttga tttgaaataa
    13681 catcaaattt tacattatct aataatgcaa aaagacttcc atcataatta tcaaaaatat
    13741 tataacctac aagtttatca tctgtataat caatataagg attgcttttg ttatcccaaa
    13801 tataagtgtt ccacccttca ttgtaatatc ttttagcaat ttcctgacct ataagaccat
    13861 agccaccgag gataagtatt gattttgtca tacaatatat ccttcctttt tagcatcaaa
    13921 tagcatcata cttttaacta attctttaaa acttattttt cttttccatc ctaattcttt
    13981 ttctgcttta ctacaatcac caataagtaa attaacctct gccggtctaa aatactttgc
    14041 tgatatttta attataggtg ttttacttcc tacaatataa acttcttcat ttaatccttc
    14101 ccctttaaat tcaattttaa tattaagaac atcaaaacat tcctgtataa actctctaat
    14161 agttcttgtt tcacctgaac taattacata atcttcaggt gtttctcttt gaagcattaa
    14221 ccacattgct tctatataat cctctgaact cccccaatcc cttttagaat taatattacc
    14281 taattcaaaa aaatcaattt tacctgttaa gacctttgct atattttttg taatttttct
    14341 tgttacaaaa gattcccctc tcaatatacc actatgatta aatagtacac ctgaacaaga
    14401 aaaaatacca taagcttccc tataattttt tgttaaccaa aaagaactta gcttagccac
    14461 cgcataaggt gagcgagggt agaaaggtgt tgtttctctt tgtggcactt cttgaacttt
    14521 tccaaatatt tctgaagtac ttgcaaagta aatttttgac tgcggagaaa attgtcttac
    14581 tgcttctaaa aggttcaagt gaccatttgt gtttatttga aaagttgaaa ggggggtatt
    14641 aaatgattca gcaacaaaag acattgcaca caaattatat atttcatctg gttgatacgt
    14701 tttaacaaca ttggaaacat ttgaaaaatc tgttatatcc ataatcacat gagtaaattt
    14761 tcccttaata cccaattctt catgtctcca aaaatcaggg gaagaagaat atctatctgt
    14821 tcctattact gtataatttt tattaagaag aaatttaatc aaattacttg catccattcc
    14881 tctacttccg gttactaaag ctactttttt attttccatc acaacactcc ttctaaaaat
    14941 tcatttttca taccatttag atattcaggt aatttctctg taccttttag tgcatcttca
    15001 taatatttca ttttatttgc tttccaagca ccataataca tttcaagatt atacagtatt
    15061 gaatttaatt ttggtaatcc ataattagaa ttaattttat aatgatattg actcatacca
    15121 taagaaggaa ctaaatcaca atctattgga gcatttatac ttttacagta ttcacctact
    15181 tcttttgttg atgaaaaatt tgtgtaagtg tgtggaatac ctacaagatt tgattcaaga
    15241 ccacataatc caaaaccttc acctcttgaa cactgtaaaa gaagagaact atccttatat
    15301 aactgaataa ttgttttatc atcaatatca taatccaata aaaaaatatt tggttttcta
    15361 tttaaagtat atgagtttgt ttctattatg ttttttgcta aaacaggaac aggaatttct
    15421 ttatctgaaa gattatgata tttacaaatc attcttacat catcttcttt tccatttgaa
    15481 aatatagaaa aagctgtcaa ggcatcttca attccttttc tttttgtatt ttgccccata
    15541 aataaaactg taaattgttt ttcttctttc ttatctgtta aagatagtct tcctttatac
    15601 aaagaataaa aactgtcttc aatatagaat ggtattttat aacaaatctt ttgagtaaga
    15661 cttttcattt cagttttaca aaaatcagaa gtagttgcgt atccatcaaa gaaattattc
    15721 aatactttcc aataatttgg aaaaggagag gtttcccacg ctatatgcaa atatgttttt
    15781 ttaaacaaat cctttttact tttatatata gaaagaaact ttttatcaaa tagaaacata
    15841 ttaggatgac attgaaacaa tacaatatca aactcttcat ctatattctc aaattcagaa
    15901 acatgacaag aagtataatt ataattatac tcatgttttt ttaatttaaa gatataatca
    15961 agattatttg ctattttatt atatccacca ttaattgcat tatctttacc tacaggagca
    16021 acaagaagta tttttttcat tagaaccatc cttcttcatt ttcacatttt gttttatttt
    16081 cttttttaac tttttctaat tcttttcttt cttctttcat aaatttaaaa ttatgttctt
    16141 tccaatactt ttctcttttt tcacctatca aaaaataatc agaatttaat tcacaagata
    16201 ttatattttc ttctaaaaca ccattggaca acaaaccaat tctttctgac cttgtaccag
    16261 aaaaaggatt aaatactgtg aagtctctaa gattttcttt aggtaaaata aataattgtg
    16321 taattttttt attaagctcc aaatttttta aagtagggtg attatttttt tgtgttaaat
    16381 tttcttcttt aatattataa taaatttccc cactacaacc atcatgatta tttgtaccat
    16441 taattgtttt gttacatgtt ttacaaattg gtgattgtga ataaattccc tgaccataac
    16501 gttttttatt atttaattct tcacaaccag catttctctc agcattgcta accttagtac
    16561 aatatgtgag tatatcaaaa tttccttgtt catataaaca atcttgtaat atatattctt
    16621 cctctgtaaa attacattta tgaagaatac gagaagcacc acctatatca ttaaaacctg
    16681 aagaatctga atttttatat tttccatatt ttctataatc cttttcagca tattcttctt
    16741 gattattatg tctaatagaa tcagaacttt tttgtatacc actttgatta tctattatat
    16801 cacttgtatt atatcctatt tcctctttaa gtctttcatc atctactata aggagttgag
    16861 ctggatatct gcctgaagta gagccatagt tttgttttat agcaaattca tggaaaggac
    16921 gtttatatcc ttcatttgca gaacttgcta aacccaaaca ttctcgctgt aaattttcct
    16981 ctgtaagaac tctccctcca tctatattta tcaaggaagg gtgtatttct ttattaccat
    17041 tttcatattt atatattgca tctatatttg acataccttt tggataaggc tttttaaaaa
    17101 tcattattgt ttcaagaact tgtttaaatg gtgaaataga aactttatac ccatcatact
    17161 ttttagcaag gttgcatgaa ggtgctgtaa tatcactaca atcaatacga cctgaccccc
    17221 ctttagcgaa attattattt ctaatatcca taattggctt acttgctctt ccagttctct
    17281 ttcctataat ttccctttca gcaccttctc tcttatccaa caacttacca acatcgcttg
    17341 cctttggaaa actcttaatc ttataagaat acaaatattg ttgtggttca aatcctgcta
    17401 attgtgcaaa gtattcaaaa ggcatttgaa ctctatcaat accatacaaa actacataag
    17461 caccatattt acaaatacga aaacattctt taaaaaatat ttctaattgt tcacatgtta
    17521 aaccattcca cttatccccc ataaattcag aaagaattac ttcttgaggt ttacctgttt
    17581 cgggatttat tctatattta ctccccatat tataaggagg gtcactaaaa acaaaactta
    17641 tagaattatc ttttaatgtg gggagtattt caaaaacatc cttattataa ttcaacttaa
    17701 accttcctta aaaaatattt aactttttta gaatttttca taacttcatc tatttgaact
    17761 tttattttat ttacacaatt ttcccatgtc aaatcttttg ttgctttatt acacctttct
    17821 aaaaaatctc tataaatacc cgaattactt tttattatat cttccatact ttgcatcatt
    17881 gtagaagcta atcttatact tgggtatttg aattttataa aattatttct tgctgataca
    17941 gattctattg tattaacagg taaaccacat cttttcccta tttcctcaat cacagtctcc
    18001 cttgaataaa ttaaaggttt accacacaat aaggcatcaa tagctccaag accaaacccc
    18061 tcacctactg ataaagaaac ataacaatca gaaactaaat atcttttata caattcttct
    18121 tttggtaaat cctcattctt tctataacaa tcaggcacta caatattttt tgttattcct
    18181 aaatttgaaa tcattgtcaa aaggtctgtt cctgaatcat aagtttcgtt tatgttggaa
    18241 tgaacatata atttataaaa ctctctgtct tgttcttctt tacacattaa aaatttatga
    18301 aaagcatcta taacaaaatc aattcttttc ctctctgtat tttttccaag tgtcataaac
    18361 aaaaaatcat catcccgtac tgcttcccca aatacatctg attttttaag tttatctttg
    18421 tcttttattg aattaagaat ttctatatcc aaaccaagat aaatattttc acaaaatttt
    18481 acaccaaaat tactaagatt attttctcct atttttgcaa ctggaatgtt catatcagcc
    18541 tttgctaaaa gctctcttat gtttattctt gcttgtttaa atttaaaaca aggaaaataa
    18601 caccataccg gataatcatt aacttcaaaa gttaaataag taatccattt ataattctct
    18661 ctataatctg agtatgctat tggttcaagt aaccaagcat caacaaaact aaaaaccaaa
    18721 tcaggtttat aatgttctac tactctgtca aaaatatcct gtttgtttgc atccattggt
    18781 gaaataaatt caaaatccat tttatcaaaa agatttttat attgttcacc atgaaaaata
    18841 taatcttctt ttttagaagg ttgtcctgta aaggtacaat aaacaatttc taaatcttta
    18901 tattctttat ttaaaccagc aataatattt aatattacat gccccatacc tgtaacatgg
    18961 tttaatgaat cagaacaaaa tagtattttc atttaataat tctccttcaa aataaataat
    19021 aactgtcttt gcttttttgt ttattttcaa attcttcttt tgtttgattt gagaataaac
    19081 attcatttac tttagtactt cctaatggag tggcattcat tatagtctca tactcaaaaa
    19141 ctttt
//
LOCUS       DAOPCH010000011        18917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_3013_length_18917_cov_62.359877, whole genome shotgun
            sequence.
ACCESSION   DAOPCH010000011 DAOPCH010000000
VERSION     DAOPCH010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 18917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18917
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3013_length_18917_cov_62.359877"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2410)
                     /locus_tag="PLU55_01405"
     CDS             complement(<1..2410)
                     /locus_tag="PLU55_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86748.1"
                     /translation="MYIYFDSNGVLREIINDKAVRQGDLNANEVLVYIENVEPADIWI
                     TFRKPGGAFSTEKSFITNKITKEIPYNKNRDMRYFKDLTEYTFYKFVISDEITEAGTY
                     EATIRFTYADSLVALGKFVFNAEPSVIKQDNLITQAQYNYLIWYVGSWQGQIGNLASG
                     IATLNSEITTIKSRLDTNEANITNLLSNYVKKTSLTTNSSQSIKPVVNGDFITFEIRN
                     LQSSDNLIKTDNENGIYVSANDVISLVSARIVSDSNLRNAIKGVVYEVGTTKDWQSGT
                     QVNSKITTHDENTTAHAYLRGLINDISQEIARIDARGRSYGELDKTLSELLLESDKDL
                     YIYNYLTNKYSGYENQVGNLIYTKVSDNEPEHELEYNGTNWVDNGAYLISKADNTQFG
                     MVKGDNEYISILNGLIQVLKSDYATRLGTSGANYTYSDLLNTLTQFATDIANRYTKAE
                     TLFELEQRITDLLSASVLQDTRITYNGQDTFTYTNQTPVVFPHSVFSEFSWAIMRVEE
                     SGIVEYTLLKPAQAVNGIKLELAMFSKDEYASFQLVNGNWQFSVYTKNGNLANTTATL
                     RIWGVKLGNINANEVDYTSDKTVEEALDEIFVPTYVTATHEALQTEITATDAFEINDE
                     TGDVIKITGNEPTQLLTNPNFDNGTTGWIAEKSSLSVSNGILTITPLGTASYAGFYQT
                     IQWTAGEEFYNKVKIRTTTPNIARFRFYIWTDENRDYIPAVINPVQNQWYTVSAIYTP
                     NVTGIQLKIHGSKNDIITNEQFVNEIIEIDYVMSYNITDFKKAGIKNDNGIPFANLTN
                     EEIKS"
     gene            complement(2415..3275)
                     /locus_tag="PLU55_01410"
     CDS             complement(2415..3275)
                     /locus_tag="PLU55_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86749.1"
                     /translation="MKEQLTTSKLLDKLYKHISWIIIAISIFGYLAYRTLRVEGSIEM
                     ILTDLNTWLNIFFVTWLNLNIQNSAINSGISAGIESQEFKLADELNNKIIKSVNNELK
                     DFRHYVRQLNKEAKTQVEDDYLFKCGVDTYDELTDKQKKKFNRLKPIRHDISGFNLPL
                     YYDITHNGEIKYESTFNKQKGIWQKRVGKILNGILFGVMSVNVVLNVKGLGEALISVG
                     VISSGLIITYLLSFLPPMFKLKFVIPKGVLLKQTLYKSYVEYKTQTHSLSEFNTNNIS
                     NDKIKTTSEV"
     gene            complement(3287..4033)
                     /locus_tag="PLU55_01415"
     CDS             complement(3287..4033)
                     /locus_tag="PLU55_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86750.1"
                     /translation="MKQSTKKLLYKIVSSLTLVLPISITLVVSAIYSKPIPNVVILFE
                     SATPVDKIADIETHFVIIQDDKYLEIDNSRGAMDVQYLNNDFTLNKNVSEFDGIKKLT
                     LKSGSYMRFITDDFDEIVEKDKQNKLVAVSIFNVETGNKLSLAFIVSILSTLIVVAVI
                     SGKMQLQKKYPKTAVFIALLSGTIVLSVINAIIGNILAVFIVATVSWGLYCLEHYYFY
                     NVIDNQIEAEGKATFSKLAEQLANELKESK"
     gene            complement(4017..4517)
                     /locus_tag="PLU55_01420"
     CDS             complement(4017..4517)
                     /locus_tag="PLU55_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86751.1"
                     /translation="MSKQQLVNKKRKDIKGTIAKIVRIVSLIISFGFVALAGIEVISG
                     YLGDVSLFGFNLTVAQQQITHYITSGGLALTGGFGIGLNEYLNRKLNQARTLVNEAMK
                     ITLETLNQVNEYQKSIGVKYDKIKMELENNNKLLTAFLETRLESKLISQETRAIIEGV
                     LNETKH"
     gene            complement(4510..4782)
                     /locus_tag="PLU55_01425"
     CDS             complement(4510..4782)
                     /locus_tag="PLU55_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPJ86752.1"
                     /translation="MAKIVLDVDLSQVQDGSILVYNIKSKHLVATNKSAFLNDLYSKI
                     NRQQQEIEVLKAEKNILEAELESLRNKVNDLVVIFENTIKEYLGNE"
     gene            complement(4800..8981)
                     /locus_tag="PLU55_01430"
     CDS             complement(4800..8981)
                     /locus_tag="PLU55_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86753.1"
                     /translation="MIYINNKQVSNLINNFVLSDEFNETLDSGIIKLHNIDKLSSLKP
                     YDDVRIEIAKDLISIPFTIEKGFEIIETKTGSLSNQVSYDAFNYVSVSNYETKTSFSG
                     NAIDGCIRYLKGLQTDYLLDTPLTIFINVEKLSDHTITSVEFVGSYSQTNPNIITFTS
                     SVYGWSINAIIDYNNDKYSFYILKIPIMRYSGSTYIVRDAYIDPQATGQYATEITYER
                     TKEITTLTIDSIINTYEMVDNVNNRISIDNIEYQLKRVNDTDYQAVYNSTTLLFNNRV
                     LQLEGGDHVIDYIYIERTINTPILLPIIVKRYLVDNYNEVFNKATNKYSYTINLMSET
                     KGLEVIQLPNISITQPIKNSKKISVWQYLNRFLTQYNPKIKVIKNSSEWEYQGKYKLD
                     PALENIYKKVYAPDFSLNNPNLRDLFTNLMITKDRLPTVRNNVIGAMDLTERKGIFNE
                     KGLIDIQSSMSSETYTQNLKKSYQNALSQDNAIRLVEKLGFRNSDEALLTLSNLRLET
                     RYPIYNINKVLMCYYKRVRIKATSDGTETIAQILCKQDITKIVIPNEKRNLLSKDWQD
                     FETDMPNTIDELAQYRLATIGYDIGSNFITGWGTSYEFPSGWWSTTEKTYIENIVRVV
                     DLSISPLGVNAYNLIRDNLEKGSIIEPYSTTNYHLNIISPFSDNNKVGALKLKGIFFE
                     IDYNGFYNGSLIHSKDNDFGNITINDNPSSSLTLLESDGVFQKEKVNRLGNEIVALAS
                     NHESLQDIQELGSVYGDDIVIFKRDISFNNNIIKANYYGSKDYVLKNYFTSVYAKHRT
                     TNLIPFDESVIKAENKKVYLYLSKEHYIVDNYLDIKFTQFDDFVEFDILSAFSPSELL
                     TSKDDFKTPKKINSGMFYKSGKGYLSDISRFVSGNSLGFNIKMYDNVSGGIYIKEKEL
                     SQVGSMQDWFILVDDIETGQIENIGIYFRHIDDINEYETLPKEYDYNKVVNAYNKLFK
                     LPEQNITLSNCTNLIGNNYKVNKDNKELLDYTLQIETITKDKDIIITPKLMELSDLIG
                     NINKVDKTYTIQDVKGGGFTDQVYVSTHQFVRVVGSEPTYAYDGTLQLNIPNNQINNL
                     AVNNVVNFIAHWNNDDGFILDEPLKSKLEIIAEKIISISTNEIKISGKATAYTRTYLF
                     FIPLKWQLVYQNKDITMTFKKLGTGTSSSNPLFYNQSHRDSNTTSYGCVIDRVNFQLI
                     AISNGLYNGYQNPSANNCETITGFYVLENLDTITTTYQKNMFVINRKEHIKKHAISEE
                     LTDSDIDKNNPNGSSIYYMNTTKQISDIFTYDVYNGKPCINIDLTSIQFNENLKTMEF
                     WFYDLKSSSYKLVFAVNVKDVDRARGFIRIYISMLNNGDLRVYNQSHDLIGEVKKLTD
                     NEFNNKIQNYCIIEL"
     gene            complement(8988..9503)
                     /locus_tag="PLU55_01435"
     CDS             complement(8988..9503)
                     /locus_tag="PLU55_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86754.1"
                     /translation="MAETRKITIEITQSGEKGQDHVLDKLNNSGDTKATGKASSESLV
                     STILVNQAFNQVKGIVKNVATYSINKHFTLKENYLMQQALDNTINTISRVGSFALTMV
                     GGAKLGSAGGVWGAIIGGTIASVGWGINEGFNIYKKLDQSQMALNQTNAEIVFSRIRA
                     GLTNESRGTIN"
     gene            complement(9517..10338)
                     /locus_tag="PLU55_01440"
     CDS             complement(9517..10338)
                     /locus_tag="PLU55_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86755.1"
                     /translation="MSNYSYNEKVILLIETNLKRIVIGSPNIFNNDIEIVVGNERYIE
                     SELTRNEPNRIYVVVQFLESAITAGHTVQPIQLVVLSEHDNLSKVQTLLRTFAEIYNT
                     KLNDDGSITQFYLTPVLLSAFNEVYDGYRGLYTLNGTLLISPNANHLTNVNVYNELTQ
                     EWEELELLSSDIDYTIQLSPDTYFDEKGQTKAHAIQATLTINLVFYLTDNNYLTNAIL
                     DDAFNETLNAFTTFKFNFVYKNKAYNNIEMQLANFLNSNKLGQLPLITITLTRGK"
     gene            complement(10331..10672)
                     /locus_tag="PLU55_01445"
     CDS             complement(10331..10672)
                     /locus_tag="PLU55_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86756.1"
                     /translation="MNNINNLDLASEIINGLKDEFAEVFLSGNLRDTIKVKINANSIE
                     IDIPGEMYDIEKYKDEGVIIYTGKGSYAQAVNITGGFSGKHTSYLENQITNAINRWIK
                     KHGLKARVYYE"
     gene            complement(10669..11031)
                     /locus_tag="PLU55_01450"
     CDS             complement(10669..11031)
                     /locus_tag="PLU55_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86757.1"
                     /translation="MISIVSKREYNEVGLLLRKVENNNKDSDNNNENSTFQYRFRFRE
                     ITATTKDNNIVGNLILTTATNITIKTSDDWGYRVNDKIRLRGSEWRITNLQKTAKISE
                     YMTRPKYIYYLQLKGIGK"
     gene            complement(11038..11949)
                     /locus_tag="PLU55_01455"
     CDS             complement(11038..11949)
                     /locus_tag="PLU55_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86758.1"
                     /translation="MEFKTRFITKDEFKLYFGIDLEIELPESDNSSDRVNAFLCRIEN
                     LLEAWINTNYFYRLNTFWETFTDYQKEQYKLALLEQAYYVLKNGDISSDSGYDKDKGI
                     VADRRSLNVIAISPATYNHLDNGGFLNKHIYKKGTGTINAMSDTYAIQTQQVIQQKGD
                     PGKDGLTPFIADNGNWWLGGVDTGVKAQAIDGAKGDTGLGFVAIFNQSVENWLTSNMF
                     SSDTIKNYLILYSGWYGDKKIEIKDNENQQTTTLTGDELVGKVLTLQLTSQRIYIRLN
                     GVLQKEYEIEDFDIDLTLTNIKLLELA"
     gene            complement(12081..13352)
                     /locus_tag="PLU55_01460"
     CDS             complement(12081..13352)
                     /locus_tag="PLU55_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86759.1"
                     /translation="MANAIELIKKYLSQAVVLTFAQKSKSRVLETDGKRIQLDFAGGN
                     TVKILNLQMEGLSDYKRANNSQITAAGGYSEFGAGDQGGRGYLGSDVGATWEAKTLRY
                     DRGAQLKIDAMDDEELAGQLMGTVVGAYIDQHIIPEVDTLRFATLAAQTTTLIGNRKE
                     AAIGDNAIISEFNLAIEFMSEAEVDGENQVFFVNPSVMSQIRNTTELQRKLTQVEYKS
                     AVEGVSFTITKYEGRVIEEVPTSRFYTGAVAGVRGFYPSATSKIINFLLVDKSVVLPV
                     VKVDNVRVFTPDQVQDFDGYKINFRVYHDLFVPERLRTGVYAHVSNTLATTKTAKLNV
                     ALSAGASGKTKITDYATLPLGHTGSIYGLAGDSGFEVGKGISEYASAVAITVSTDFTA
                     FSGDKGYFALVNARGVIIAVSTKYTNIPKGT"
     gene            complement(13363..13560)
                     /locus_tag="PLU55_01465"
     CDS             complement(13363..13560)
                     /locus_tag="PLU55_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86760.1"
                     /translation="MEKIKELLAALLEQGLTDDEVVKVLDQKLADQEITQEEHDWAIQ
                     ELAKLKEQTELKQALELLGVE"
     gene            complement(13573..14214)
                     /locus_tag="PLU55_01470"
     CDS             complement(13573..14214)
                     /locus_tag="PLU55_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86761.1"
                     /translation="MTLEEIKSGQSKVETELPIPKIEDKLEPPKETAPEPKGDEKPNP
                     QPQQEPQPQAEQPKSADNDDNLDDPKGSEKTFTKTDVNEIIQRRLKRQKEKFHEQLGV
                     DTDEAVVELVNKLKGYDELLNSVETLKTENNALKEKMLFIDNNISQKRYDDVRTYFKG
                     KEIELTTENLLKELKTHPEWVASTKPVQIGSQKQEAPVNDEEKLAMRLFGVRK"
     gene            complement(14261..15232)
                     /locus_tag="PLU55_01475"
     CDS             complement(14261..15232)
                     /locus_tag="PLU55_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86762.1"
                     /translation="MKAQLIQIQRNKAKETKRLLSVKNKIIVSIYEMVINNKNNNALH
                     TRLKKETLQDTFLYKPALRISKQIKREIDRSKNTTIKSSGDDKKLDNQEWVATLFLLA
                     INKLQVSNKLSKCVYKETRKQESEAKEKLLKEMLSSKRSINKDDPKVFFLVSKHSDCA
                     KDHIEYQGQIYIDENWKKLISNSNNELKVAIENYIRINNIKSYQWVIDKPVWMITRPN
                     CRHFAKVLSVSEVLNNPTWQLLVKNKMTMKEGDRELRQTLRHNMSSKKGNYNEKNINA
                     IIDKYKERLELHTELNKVKSTQELRHAITKDKFLIKKWQNLLKSLTN"
     gene            complement(15198..16799)
                     /locus_tag="PLU55_01480"
     CDS             complement(15198..16799)
                     /locus_tag="PLU55_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86763.1"
                     /translation="MLIIDWIREKIKSFLQIEHIHNNPNTSTFQYISDQEKNRIAKAH
                     ELKLWYMGNSAEIEYFYMKKDFIKPSDYATTNYFWARALREAAVKKVHSGLANATVIT
                     LVNVIGDHIIKSQNTEVQKLIDNIMDYNDFTKMVYQEQLPLTIAQGWGAFKFNIDKQY
                     KYPLIEYYEAENVRFIGKNRRIEAIIYLNYYEIDKKKYVLFETRSLKQDNETKGIKAG
                     SYVEYNLFLLGTDNNITPVKLSEVPELSNLQPIFIPSYKNILGVPCRILHNPNDKYYG
                     LGIFESKIDILDDIDQALSQASQTVRVSTPVEYYPTSLLEKTPDGNPVLPKAYNRQFI
                     EAPSNLPGPDGTVGSERIQTTQPQLNFNQYQEEVKNKIDIFLTGILSPATMGIDIAKK
                     DNAEAQREKEKITLMTRNNIIASQRKIIKEVMEIALDLTEFMQTDKITFDKEYDFSVI
                     YNEFGSPSFESKLQALTPAYNSGAISTERYVEALWGDMLNDSEKQEEINKLNEIRARD
                     NLDLGSFESDDIYLDESSIDTNTTE"
     gene            complement(16864..18345)
                     /locus_tag="PLU55_01485"
     CDS             complement(16864..18345)
                     /locus_tag="PLU55_01485"
                     /inference="COORDINATES: protein motif:HMM:NF016359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HPJ86764.1"
                     /translation="MLKTSSNSNDNNSYTLSMQKIIGKGYNNGWWTNCKARYRLYKGA
                     RNSKKTYNMLGQESINKILTQPLRNILIIRQTENSHKITTWNTILSLINQPDFRNPEI
                     SLSRFFKVNSVDKIITYIPTGQVIVFRGFDNADKLTSLKATVGFFTDVYIEEAFEIKD
                     YDEFRKLDGSIRGKLPKGYFFQITFLFNAWNKEHWLYDKFFKGNLEDDYQELLLNDYM
                     DYHDPNYIGDYGKGLYLHISTYKVNEFRDTNIYDLAMDNLRIKAPEIYKVEALGMWGV
                     VGDRLYPEWNDSLIAEPQKIMLTRFGRYAIGIDTGLSDATKNKTSKSATTMVLSGLTW
                     DRKRIIATDEYYHSNAVKKKTEPEIMQEIINALKMWQIKYQSHQDLMKGQVFVYVDSA
                     DIGFREGLEYLARQNNMYNFVFLGSTKYPISRRVYFIRVLMAHGNYLVSSLCPNLIRE
                     HKTAVSGEKGEIRLIGDDHTQDAMEYSWSSYVNELQLWHTIKI"
     gene            complement(18329..18868)
                     /locus_tag="PLU55_01490"
     CDS             complement(18329..18868)
                     /locus_tag="PLU55_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86765.1"
                     /translation="MEQQKEIKKDNTIKTKTTNKPKVSKKTTTNTKAKKTTTKKSTTT
                     KKKVDKRGNKALLVAHPKKVKLFLKAVSLGMSDADACRRSGIAVATIQDYKWKGNQDL
                     EKGKDTIYSQFMELYNNAYSSFIEVNLQNIMNAGLQDWRASHALLKMRDAKNYGDKQE
                     ISINADELGIKIINDVKDK"
BASE COUNT         5775 a         3320 c         2245 g         7577 t
ORIGIN      
        1 gtgatttgat ttcttcgttc gttaagttag cgaatggaat accattatca tttttgatac
       61 cggctttttt gaaatcagtg atgttataag acataacata atctatttct attatctcat
      121 taacaaactg ctcattcgta ataatatcgt ttttactacc gtgaattttt aactgtattc
      181 cagttacatt tggagtataa atagcactga ccgtatacca ttgattttgc acaggattta
      241 taacagcggg aatataatct ctattttcat cagtccagat ataaaaccta aatctagcaa
      301 tattcggtgt agtcgttcta attttaactt tgttataaaa ttcttcacca gcagtccatt
      361 ggatagtttg atagaaacca gcgtaagaag cagttcctaa tggtgtaata gttaaaatac
      421 cattgctaac agataaagat gatttttcag caatccaacc agttgttccg ttatcgaaat
      481 taggattggt taatagttga gtaggttcat tcccagtaat cttaatcaca tcgccagttt
      541 catcattaat ctcaaaagcg tcagtcgcag ttatttctgt ttgtagtgct tcatgtgtgg
      601 cagtgacata tgtaggaaca aagatttcat cgagtgcttc ttcaactgtt ttatcacttg
      661 tataatcaac ttcgtttgcg ttgatattgc ctaacttaac accccaaatg cgtaaagttg
      721 cagttgtgtt tgctaaatta ccattttttg tataaaccga aaattgccaa ttaccattta
      781 ctaattgaaa acttgcatat tcatcttttg aaaacatagc aagctctaat ttaatgccat
      841 taactgcttg agctggttta agtaaagtgt attcaacaat tccgctttcc tcaacacgca
      901 taatcgccca actaaactca ctaaaaacac tatgcggaaa tacaaccggt gtttgatttg
      961 tgtatgtaaa tgtatcttgt ccgttatatg taattcttgt atcttgtaaa accgaagcac
     1021 ttaatagatc cgttattctt tgttctaact caaataatgt ttctgctttt gtataacgat
     1081 tagcaatatc cgttgcaaat tgcgttaatg tatttagtaa atcactataa gtataatttg
     1141 ccccacttgt gccaagtcgt gttgcataat cactttttaa tacttgaatt aaaccattaa
     1201 gaatacttat atattcatta tcgcctttaa ccataccaaa ttgcgtgtta tcggctttac
     1261 taattaagta tgcaccatta tcgacccaat ttgtgccgtt atattcgagc tcgtgttccg
     1321 gctcgttatc actaactttt gtatatatta aattgccgac ttgattttca taaccactat
     1381 atttattagt taaataatta taaatatata aatctttatc gctttctaaa agtaattcgc
     1441 ttaaagtttt atctaattca ccataagaac ggccgcgagc atcaattcgt gctatttctt
     1501 ggctaatatc atttattaac cctcttaaat aagcatgtgc cgtagtattt tcatcatgtg
     1561 ttgtaatttt agagtttact tgtgtcccac tttgccaatc tttagttgtg ccaacttcat
     1621 acacgacacc tttaattgca ttgcgtaaat tactatcgct tacaattctc gcacttacta
     1681 aactaataac atcattagca cttacatata ttccattttc attatctgtc ttaattaaat
     1741 tatcactgct ttgtaagttt ctaatttcaa aagtaataaa atcaccattt acaactggtt
     1801 taatactttg actactattt gttgttaaac ttgtcttttt aacataatta cttaataaat
     1861 tagtaatgtt tgcttcgtta gtatctaatc ttgatttaat agtagtaatt tcactattta
     1921 atgtagcaat tccacttgct aaattaccaa tttgaccttg ccaactgcca acataccaga
     1981 tcaaataatt atattgagct tgagtaatta aattatcttg tttaataaca cttggttcag
     2041 cgttaaaaac aaacttacct aacgcaacta aactatcagc ataagtaaat ctaattgttg
     2101 cttcatatgt tcccgcttct gtaatttcat cactaataac aaacttgtaa aaagtatatt
     2161 ccgttaaatc cttaaaataa cgcatatctc tatttttgtt atatggtatt tcttttgtta
     2221 tcttattagt aataaaactt ttttcagttg agaacgcacc acctggcttt ctaaatgtta
     2281 tccaaatatc tgccggctcg acattttcaa tgtaaactaa tacctcattt gcattcaaat
     2341 cgccttgtct aactgcttta tcgtttataa tctctcttaa aacaccgttg ctatcaaaat
     2401 aaatatacat aattttatac ctcgcttgtt gtttttattt tatcattact aatgttatta
     2461 gtgttaaact cacttaatga gtgtgtttgt gttttatatt ctacataact tttataaagt
     2521 gtttgtttta acaaaactcc tttaggaata acaaacttta acttaaacat tggtggtaaa
     2581 aatgataata aataagtaat tattaaacca cttgagataa caccaacact tatcaatgct
     2641 tcgcctaacc ctttaacatt taagacaaca ttaacactca tcactccaaa taaaatacca
     2701 tttaatattt ttcccactct tttttgccag atcccttttt gtttattaaa tgtgctttca
     2761 tacttaatct caccgttatg ggttatatca taataaagtg gtaaattaaa gccgcttata
     2821 tcatgtctaa ttggttttaa tctattaaac tttttctttt gtttatctgt taattcatcg
     2881 taagtatcaa ctccacactt aaacaagtaa tcatcctcaa cttgtgtttt tgcttctttg
     2941 tttaattgtc ttacataatg tctaaaatct tttaattcat tattaacact tttaataatt
     3001 ttgttattta attcatcggc caacttaaac tcttgacttt caataccagc actaattcca
     3061 ctatttatag cactattttg aatatttaag tttaaccaag ttacaaaaaa gatattcaac
     3121 caagtattaa ggtcagttaa aatcatttct atagaccctt caactcgaag tgttctatat
     3181 gctaaataac caaaaatact aatcgcaata attatccacg atatatgttt atataatttg
     3241 tctaataatt tacttgtcgt taattgttct ttcataattt atttatttat ttgctttctt
     3301 ttaattcgtt tgctaattgt tcggccaact tgctaaatgt tgctttgccc tctgcttcta
     3361 tttgattatc aattacatta taaaaatagt aatgttctaa acaatataaa ccccagctga
     3421 ccgttgccac gataaacacg gccaaaatat taccgataat tgcattgata acacttaaaa
     3481 ctattgtgcc acttaataaa gcgataaaaa cggctgtttt aggatatttc ttttgtaatt
     3541 gcattttgcc actaataact gctacaacaa ttaaagttga taaaatagat acaataaatg
     3601 cgagtgataa tttattacca gtttcaacat tgaaaatact aactgcaact aacttatttt
     3661 gtttatcttt ttctactatt tcatcaaaat catcggtaat aaaacgcata taactaccac
     3721 tctttaatgt taacttttta atgccatcaa actcgctaac atttttattt agtgtaaagt
     3781 cgttgtttag atattgaaca tccattgcac cccgtgaatt atcaatttct aaatatttat
     3841 catcttgaat aattacaaag tgtgtttcaa tatcagcaat tttatcaacc ggtgttgcac
     3901 tttcaaataa aattacaaca tttggtattg gtttagaata aatagcacta acaacaagtg
     3961 taatacttat tggtaataca agcgttaaac tgctaactat tttataaagt aattttttag
     4021 tgctttgttt catttaacac accctcaatt atcgctcttg tttcttgtga tattaacttg
     4081 ctttctaatc ttgtttctaa aaaagccgtt aataatttat tgttattttc taattccatt
     4141 ttaattttat catatttaac accaatgctt ttttgatatt cattaacttg atttaatgtt
     4201 tcaagtgtaa ttttcatcgc ttcattaact aatgttcgag cttgatttaa ttttctattt
     4261 aagtattcat ttaatccaat tccaaaacca ccagttaaag ccagtccacc actggttata
     4321 taatgtgtaa tttgttgctg tgctaccgtt aaattaaagc caaaaagtga tacatcacct
     4381 aaataaccac ttataacctc aattccagca agtgcgacaa atccaaagct aattattagt
     4441 gaaacaatac gcactatttt tgcaatcgtt ccttttatat cttttctttt tttattaact
     4501 aattgttgtt tactcattgc ctaaatactc ctttattgta ttttcaaaaa taactactaa
     4561 atcatttact ttattgcgta aactttctaa ttcggcttct aaaatgtttt tttcggcttt
     4621 taatacttca atttcttgtt gctgtctatt gatctttgaa tataaatcgt ttagaaaagc
     4681 actcttgttt gtcgccacta aatgcttact tttaatgttg taaactaaaa tagatccatc
     4741 ttgcacttgc gacaaatcaa catctaatac aatttttgcc ataacttttt attctccttt
     4801 tataattcta ttatacaata attttgtatt ttgttattaa attcattatc agttaatttt
     4861 tttacttcgc caattaaatc atgactttga ttataaactc gtaaatcacc attgtttaac
     4921 attgatatat atatgcgaat aaaccctctt gctctatcaa catcttttac attaactgca
     4981 aacactaatt tgtatgatga actttttaag tcataaaacc aaaactccat tgtttttaag
     5041 ttttcattaa attgaatact cgttaaatca atatttatac acggcttgcc attataaaca
     5101 tcataagtaa atatatcact tatttgtttt gttgtattca tataataaat gctactgccg
     5161 ttaggattat ttttatctat gtcgctatca gtcaactcct cacttattgc atgtttcttt
     5221 atatgttctt ttctatttat aacaaacata tttttttgat aagttgtggt aattgtatcg
     5281 agattttcca aaacataaaa accggtaatt gtttcacaat tattagcact tggattttga
     5341 taaccattat aaagaccatt tgagattgca attaattgaa aattcactcg atcaataaca
     5401 caaccataac tcgttgtatt actatctcga tgagattgat tataaaataa cggattgctt
     5461 gaacttgtcc cagtgcctaa tttcttaaaa gtcattgtta tatctttatt ttgatatact
     5521 aattgccatt tcaaaggaat aaaaaacaaa taagttcttg tatacgccgt tgccttacca
     5581 cttatcttta tttcattagt tgatatacta ataatttttt cagcaataat ttctaatttt
     5641 gacttaagtg gttcatcaag tataaaacca tcatcgttgt tccagtgtgc tataaaatta
     5701 acaacattat taactgctaa attatttatt tgattatttg gtatatttaa ttgtaatgtt
     5761 ccatcatatg catatgttgg ttcacttcca acaactctaa caaattgatg agttgacaca
     5821 tacacttgat ccgtaaagcc gccgccttta acatcttgaa ttgtataagt tttatctact
     5881 ttatttatat taccgattaa atcgcttaac tccattaact tgggcgtaat tattatatcc
     5941 ttatcttttg ttattgtttc aatttgtaaa gtataatcca acaactcttt attgtcttta
     6001 ttcactttat aattattacc tattaaatta gtgcaattac ttaatgttat attttgttct
     6061 ggtaatttga ataatttatt ataagcatta accactttat tataatcgta ctcttttggt
     6121 aatgtttcat actcatttat atcatcaatg tgtctaaaat aaatgcctat attttcaatt
     6181 tgaccggttt caatatcatc gactaaaata aaccaatctt gcatagagcc aacttgtgat
     6241 aactcttttt cttttatata tattccacca gaaacattat catacatttt tatattaaaa
     6301 cctaatgaat taccacttac aaatcggctt atatcactta aataaccttt accactttta
     6361 taaaacatac cactatttat ctttttaggt gtcttaaaat catctttgct tgttaataat
     6421 tcacttggtg aaaatgcact caaaatatca aactcgacaa aatcatcaaa ttgtgtgaac
     6481 tttatatcta aataattatc aactatgtaa tgttctttac ttaaatataa atatactttt
     6541 ttattttctg ctttaataac actttcatca aaaggtatta aattagttgt tcggtgtttt
     6601 gcgtagacac ttgtaaaata attctttaat acataatctt tactaccata ataatttgct
     6661 ttaattatat tattattaaa acttatatct cgcttaaaaa taactatatc atcgccatat
     6721 acactgccta actcttgtat atcttgtaaa ctttcatgat tacttgccaa tgcgacaatt
     6781 tcattaccta acctattaac cttttctttt tgaaaaactc catcactttc taaaagtgtt
     6841 aaactcgagc ttggattatc atttatagta atattaccaa aatcattatc tttgctgtgt
     6901 attaaagaac cattataaaa gccgttataa tcaatttcaa aaaagatacc ttttaatttt
     6961 aatgcaccaa ccttattatt atcgctaaat ggactaatta tatttaagtg ataattagtt
     7021 gttgagtatg gttcaataat agatcctttt tctaaattat cacgaataag attataagca
     7081 ttaactccaa gtggactgat acttaaatca acaactctta ctatattttc aatatatgtt
     7141 ttttcagtag tagaccacca gccagatgga aactcataac ttgtccccca tccagtaata
     7201 aaattactgc caatatcata accgattgtt gccaatctat attgtgctaa ttcatcaatt
     7261 gtgtttggca tatcggtttc aaaatcttgc caatctttac ttaataaatt gcgtttttca
     7321 tttggaatta caattttagt tatatcttgc ttacatagta tttgtgcgat agtttccgtg
     7381 ccatcacttg ttgccttaat tcttactctt ttgtaataac acataagcac tttatttata
     7441 ttgtaaattg ggtagcgagt ttctaatctt aaattactta aagttaataa tgcttcatca
     7501 ctatttctaa accctaattt ttcaacaagt ctaattgcgt tatcttgact taaagcattt
     7561 tgataactct ttttaagatt ttgcgtatat gtttcactac tcattgagct ttgaatatca
     7621 attaaacctt tttcattaaa tatgcctttt ctttcggtta aatccatagc accaataaca
     7681 ttatttctaa cagttggtaa tctatcttta gttatcatta aattagtaaa tagatctcgc
     7741 aaattaggat tgttaagtga gaagtccggt gcataaactt ttttatatat attttcaagt
     7801 gctggatcta atttatactt accttgatat tcccactcac tactattttt tattacttta
     7861 atctttgggt tatattgagt taaaaatcta tttaagtatt gccataccga tattttctta
     7921 ctatttttaa tcggttgtgt aattgatata ttcggtaatt gaataacttc gagccctttt
     7981 gtttcactca ttaaattgat agtgtatgaa tatttattag tcgctttatt aaatacttcg
     8041 ttataattat caactaaata tcttttaaca attattggta ataaaatagg tgtatttatt
     8101 gttctttcaa tataaatata atcaattaca tgatcgccac cttctaattg taaaactcta
     8161 ttattaaata ataatgttgt tgaattataa actgcttgat aatctgtatc gttaactctt
     8221 ttaagttgat attctatatt atcaatacta atgcgattat taacattatc taccatttca
     8281 taagtattta taatgctatc aatagttaat gtagttattt cttttgttct ttcataagta
     8341 atttcagttg catattgtcc ggttgcttgt ggatctatat atgcatctct cactatataa
     8401 gtactgccac tatatctcat tatcggtatt tttaatatat aaaatgaata cttatcattg
     8461 ttataatcaa ttattgcatt gatagaccaa ccataaacac tgcttgtaaa tgtaataatg
     8521 tttgggttag tttgcgaata actaccgaca aactcaacac ttgtaattgt atggtcgctt
     8581 aatttttcta catttataaa aatcgttaat ggcgtatcta acaaataatc agtttgtaaa
     8641 cctttcaaat aacgaataca accatctatt gcatttccac taaagctcgt tttagtttca
     8701 taattactaa cacttacata attaaaagca tcataactta cttgatttga taatgaaccc
     8761 gttttcgttt ctataatttc aaaacctttt tcaatagtaa atggaattga tattaaatct
     8821 tttgcaattt caattcgtac atcatcataa ggttttaatg atgataattt atctatattg
     8881 tgtaatttaa ttattccact atctaaagtt tcattaaact catcgcttaa aacaaaatta
     8941 tttattaaat tgcttacttg tttattattt atataaatca taatctttta atttattgtt
     9001 cccctactct catttgttaa accagctcta attctactaa acactatttc agcattagtt
     9061 tgattaagtg ccatttgtga ttggtctaat tttttataaa tgttaaagcc ctcatttatt
     9121 ccccaaccaa cacttgcaat tgtgccacca ataatagcac cccatactcc accagcactg
     9181 cctaatttag caccaccaac catagttagt gcgaaactac caacacgact aattgtattt
     9241 attgtattat caagtgcttg ttgcattaag tagttttctt taagtgtaaa atgtttgttt
     9301 atgctatatg tagcaacatt ttttacaatt cctttaactt gattaaatgc ttgattaact
     9361 aaaattgtag atactaaact ttcactactt gccttaccag tcgcctttgt atcaccacta
     9421 ttgtttaatt tatcaagaac atgatcttgt cctttttcac cggattgcgt tatctcaata
     9481 gtgatttttc ttgtttctgc cataatttat tatttattat ttgcctctcg ttaaagttat
     9541 tgttattagt ggtaattgtc ctaacttgtt agagttaaga aaattagcaa gttgcatttc
     9601 aatattatta tatgctttat tcttataaac aaaattaaac ttaaatgtag taaatgcgtt
     9661 tagtgtttca ttaaatgcat catctaaaat tgcattagtt aaataattat tatcagttaa
     9721 ataaaatact aaattgatag ttaatgtggc ttgtattgcg tgagctttag tttgcccttt
     9781 ttcgtcaaaa tatgtatctg gtgataattg aatagtgtaa tcaatatcac tgcttaataa
     9841 ttctagctct tcccactctt gcgttaattc attataaaca tttacatttg ttaagtggtt
     9901 tgcatttggt gagattaaaa gtgttccgtt gagtgtatat aaccctctgt agccgtcgta
     9961 gacctcatta aatgcactta ataaaaccgg tgttaaataa aattgcgtta tggatccatc
    10021 atcatttaat tttgtgttat atatttctgc aaaagttctt aataaagttt gaactttgct
    10081 taagttatcg tgttcgctta aaactactaa ttgtataggt tgcaccgtgt ggccggccgt
    10141 tattgcactt tctaaaaatt gaacgacaac ataaatccgg ttaggttcgt ttcttgttaa
    10201 ttcactttca atatatcttt cattacctac tacaatttca atatcattat taaaaatatt
    10261 tggtgaacca attacaattc tttttaagtt agtttctatt aacaatataa ctttttcatt
    10321 ataactataa ttactcatag taaactctcg ctttcagtcc gtgcttttta atccaacgat
    10381 ttattgcatt tgtaatttga ttttctaaat acgaagtatg tttaccactg aatccaccag
    10441 ttatattaac tgcttgtgcg tatgaaccct taccagtata aataattact ccctcatctt
    10501 tatatttttc aatatcatac atttcacctg gaatgtcaat ttcaatacta tttgcattta
    10561 ttttcacttt aattgtgtcc cttaaattac cacttaaaaa tacttcagca aactcatctt
    10621 taagcccatt tataatctca cttgctaaat ctaaattatt tatattattc atttcccaat
    10681 accttttaat tgtaaataat aaatatattt tggtcgtgtc atatactcgc taatctttgc
    10741 tgttttttgt aagttagtaa ttcgccactc actccccctt aaccttattt tatcattaac
    10801 tctataaccc cagtcatcac ttgtcttaat agtgatatta gtcgctgttg ttaaaatcaa
    10861 attaccaact atattattgt cttttgttgt tgccgttatt tctctaaatc taaagcgata
    10921 ttgaaaagtg ctattttcat tattattatc gctgtcttta ttattattct caactttacg
    10981 caataataaa ccaacttcgt tatattctct ttttgaaaca atactaatca taattattta
    11041 tgctaactcc aataatttta tgtttgttaa tgttaaatca atatcaaaat cttctatttc
    11101 atattctttt tgaagcacac catttaatct tatatatatt ctttgacttg ttaattgtag
    11161 tgttaatact ttaccgacta attcatcgcc agttaatgta gttgtttgtt gattttcatt
    11221 atcttttatt tctatttttt tatcaccata ccaaccgcta tataaaatta gataattttt
    11281 aattgtatca ctactaaaca tattacttgt taaccaattc tcaacacttt gattaaaaat
    11341 agcaacaaaa ccaagtccag tgtcgccctt tgctccatct atcgcttgag ctttaactcc
    11401 agtatcaacc ccaccaagcc accaattacc attatcagca atgaaaggtg ttaatccatc
    11461 tttgcccgga tctccttttt gttgaataac ttgttgtgtt tgaattgcat atgtatcact
    11521 cattgcattg atagtgccag tgcctttttt gtaaatatgt ttgtttaaga aacctccgtt
    11581 atcaagatga ttataagttg ctggacttat tgcaattaca ttaagtgatc tcctatctgc
    11641 tacaattcct ttatctttat cataaccact atcacttgat atatcaccat tttttaacac
    11701 ataatatgct tgttctaaaa gtgccaattt atattgttct ttttgataat cagtaaatgt
    11761 ttcccaaaat gtattaagtc tataaaagta attagtgtta atccaggctt ctaataagtt
    11821 ttcaattcta cataaaaacg cattaactct atcacttgaa ttatcgcttt ctggtagttc
    11881 gatttctaaa tcaataccaa aatataattt gaactcatct tttgtaataa atcttgtctt
    11941 gaattccata agtttatttt atactcaact ttcaactttc atatttttat tttagcatat
    12001 aaaaaaagga attgtatatt tttcaatttt tatacaactc ctcttataaa tatttggtta
    12061 gttaattagt tagttaataa ctatgttcct tttggaatat tagtgtattt tgtactaact
    12121 gcaataatta caccacgagc attaacgagt gcaaaatatc ctttatcacc actaaatgct
    12181 gtaaagtcag tgctaaccgt aattgcaact gcacttgcat actcgctaat tcctttacca
    12241 acttcaaaac cactatcacc agcaagaccg taaattgatc cagtgtgtcc taatggtaat
    12301 gttgcataat cagtaatctt tgtcttacca cttgcaccag ccgataaagc aacatttaat
    12361 ttagctgttt tagttgtagc aagtgtattg cttacatgtg cgtatacacc agttcttaat
    12421 ctttctggaa caaataaatc gtgataaaca cggaagttga ttttgtagcc atcaaaatct
    12481 tgcacttgat ctggtgtaaa cactcttaca ttgtcaactt tgactactgg taaaactaca
    12541 cttttatcaa ctaataagaa gttaataatt ttggatgttg cacttgggta gaacccacga
    12601 actccagcaa ctgccccagt ataaaatctg cttgttggga cttcttcaat tactcgacct
    12661 tcatattttg taattgtaaa tgatactcct tcaactgcac tcttatactc tacttgagtt
    12721 agttttcttt gtaattcagt tgtatttcta atttgcgaca taacacttgg attgacaaag
    12781 aatacttgat tttcgccatc aacttctgct tcactcataa actcaattgc taaattgaat
    12841 tcgctaataa ttgcattatc accaattgcg gcttcttttc tattaccaat taaggttgtt
    12901 gtttgagcgg ctaaagttgc aaagcggagt gtatcaactt ctgggataat atgttggtcg
    12961 atatatgcac caactaccgt tcccattagt tgaccggcca attcctcatc atccattgca
    13021 tcaattttta attgagctcc acgatcatat cgtagtgtct ttgcttccca agtagcacca
    13081 acatcgctac caagatagcc tcgcccgcct tggtcgccag ctccaaattc gctataacca
    13141 ccagcggctg taatttgaga gttattagcc ctcttataat cgcttaaccc ttccatttgt
    13201 aagttaagga tttttacggt atttccaccg gcgaagtcaa gttgaattcg ctttccatca
    13261 gtttctaata cccgtgattt tgatttttga gcgaaagtta aaacaactgc ttggctcaaa
    13321 tattttttaa ttagttcgat tgcgtttgcc ataataattc tcctattcta cacctaaaag
    13381 ctcaagtgct tgtttgagtt ctgtttgttc ttttagtttc gccaactctt gaatagccca
    13441 atcgtgttcc tcttgggtga tttcttgatc ggctaacttt tggtctaaaa ctttaacaac
    13501 ttcatcatca gtaagccctt gttctaaaag tgcggccaat aattctttaa ttttttccat
    13561 aagtttattt tcctactttc taacaccaaa taatcgcatt gctaattttt cctcatcgtt
    13621 aactggtgct tcttgctttt gacttccaat ttgcaccggt ttagtggaag ccacccactc
    13681 tggatgagtt tttaattctt tcaatagatt ttcagttgtt aattcaattt ctttgccctt
    13741 aaaatatgtt cgcacatcat cgtatctttt ttgagagata ttattatcaa tgaatagcat
    13801 tttttcttta agtgcattgt tttccgtttt taaagtttca acggaattaa gtaattcatc
    13861 atacccttta agtttattaa ctaattctac aacggcttca tcggtatcca ctcctaattg
    13921 ttcgtggaat ttttcttttt gcctttttaa cctacgctgt ataatttcgt taacatctgt
    13981 tttggtaaat gttttttcac taccttttgg gtcatccaaa ttatcatcat tatctgctga
    14041 ttttggttgt tctgcttggg gttgtggttc ttgttgtggt tgtggatttg gtttttcatc
    14101 accttttggc tctggtgctg tttcctttgg tggttctaac ttatcctcaa tttttggtat
    14161 tggtagttcc gtttctactt tgctttgtcc ggatttaatt tcttcaagtg tcattgttta
    14221 acctccaatg attaagtaat tttaatataa catatttatt ttagtttgtt aaacttttca
    14281 ataagttttg ccattttttg attaaaaact tatctttagt aattgcatgt cttaactctt
    14341 gagtagattt aactttgttt aattcagtat gtaattctaa tctttcttta tatttatcaa
    14401 taatcgcatt tatgtttttt tcattataat tgcctttttt agatgacata ttatgtcgta
    14461 atgtttgcct taattctcgg tcgccctctt tcatagtcat tttattttta actaataatt
    14521 gccaagttgg attgtttaac acttcactaa cacttaacac ttttgcaaag tggcgacaat
    14581 tcggccgagt aatcatccac actggtttat caataaccca ttgataactt ttaatattgt
    14641 taattcgtat ataattttca attgctactt ttaattcatt attactatta ctaattaact
    14701 ttttccaatt ttcatcaata tagatttgac cttggtattc tatatggtct ttcgcacaat
    14761 cactatgttt gctaactaaa aaaaagactt taggatcatc tttatttatc gatctttttg
    14821 agcttaacat ttctttaagt aatttttctt ttgcttcact ttcttgtttg cgcgtttctt
    14881 tataaacaca tttacttaac ttattactaa cttgtaattt gttaatcgcc aataaaaaaa
    14941 gtgtcgccac ccactcttga ttatctaatt ttttatcatc accactactt ttaatagtag
    15001 tatttttaga tcgatctatt tctcttttta tttgtttact aattcttaat gctggtttat
    15061 ataaaaaagt atcttgtaaa gtttcttttt tcaagcgagt gtgtaaagcg ttattgtttt
    15121 tattgttaat aaccatttcg taaatactaa caataatttt attcttaacc gacaaaagcc
    15181 gtttagtttc ttttgcttta ttccgttgta tttgtatcaa ttgagctttc atctaaataa
    15241 atatcatcac tttcaaaaga tcctaaatct aaattatctc ttgcccttat ttcatttaat
    15301 ttgtttattt cctcttgttt ttcactatca tttaacatat caccccataa tgcctcaaca
    15361 tatctttcgg tagatatcgc accactatta tatgctggag tgagtgcttg tagtttgctt
    15421 tcaaagctgg gactgccaaa ctcattataa ataacactaa aatcgtattc tttatcaaat
    15481 gttattttat ctgtttgcat gaactcggtt aaatcaagtg caatttccat tacctcttta
    15541 ataatctttc tttgtgaagc aataatatta tttcgtgtca ttaaagtaat cttttctttt
    15601 tctctttgag cctctgcgtt atctttttta gcaatatcaa tacccattgt cgctgggctt
    15661 aaaataccag ttaagaatat atcaatttta tttttaactt cctcttgata ttgattaaag
    15721 ttaagttgtg gttgcgttgt ttgtattctt tcactgccaa ccgttccatc tgggccaggt
    15781 aaattactcg gtgcttcaat aaattgtcta ttatatgctt ttggtaaaac tgggttgcca
    15841 tctggtgttt tctcaagcaa actcgtgggg taatattcta ccggagtgct aactctaaca
    15901 gtttgacttg cttgacttaa tgcttggtct atgtcatcta aaatatctat tttgctttca
    15961 aaaataccaa gaccataata tttatcattt ggattatgca aaatacgaca tggaacaccc
    16021 aatatatttt tataacttgg aataaaaatc ggttgtaaat tagaaagttc gggaacttcg
    16081 cttaacttaa ctggtgtaat attattatct gttcctaata aaaataaatt gtattctaca
    16141 tacgaaccgg ctttaattcc ttttgtttca ttatcttgct ttaatgatct tgtttcaaat
    16201 aatacatatt tctttttatc aatttcataa tagttcaaat aaatgattgc ttctattctg
    16261 cgatttttac caatgaacct tacattttct gcctcataat attcaatgag tggatattta
    16321 tattgcttat caatgttaaa cttaaatgcc ccccaacctt gagcgattgt taatggtaat
    16381 tgttcttgat ataccatttt tgtaaaatcg ttataatcca taatattatc aattagtttt
    16441 tgaacttccg tattttgact tttaataata tggtcgccaa taacattaac aagtgttata
    16501 accgttgcat ttgctaaacc actatgcact tttttaacgg ccgcttctct taatgctctc
    16561 gcccaaaaat aattagtagt agcataatcg ctcggcttta taaagtcctt tttcatataa
    16621 aaatattcaa tttcagcact attacccata taccataatt tgagttcatg tgcttttgct
    16681 attctgtttt tttcttgatc gcttatatac tgaaaagtcg aagtattagg attattgtgt
    16741 atatgttcta tttgtaaaaa tgatttaatt ttttcacgga tccaatcaat aattaacata
    16801 atttataatt tccccttttg tgttctcata attttattat acactaaaca aaataaaaaa
    16861 ttattagatt tttattgtgt gccataattg taattcatta acatacgaac tccaactata
    16921 ttccatcgca tcttgtgtat gatcatcccc aataagtcta atctcgcctt tttcaccact
    16981 aactgctgtt ttatgttctc taattaaatt aggacataaa ctactaacaa gataatttcc
    17041 gtgtgccatt aacactctta taaaataaac tcgccgtgat attgggtatt ttgtgctacc
    17101 taaaaataca aaattataca tattattttg tcgagctaaa tattctaaac cctctctaaa
    17161 tccaatatca gcactgtcga cataaacaaa tacttgacct ttcattaagt cttggtgcga
    17221 ctgatattta atctgccaca ttttaagagc attgattatt tcttgcataa tttctggttc
    17281 ggtttttttc ttgactgcgt ttgagtggta atattcatca gttgcaataa ttcgctttct
    17341 atcccaagtc aagccactca aaaccattgt tgtagcactc ttacttgttt tatttttagt
    17401 tgcatcactt aaaccagtat caataccaat tgcatatcgg ccaaaccgag ttaacattat
    17461 tttttgtggt tcagcaatta aactatcgtt ccactctgga taaagtctat caccaaccac
    17521 accccacata ccgagtgcct caactttata tatctcgggt gctttaattc ttaagttatc
    17581 cattgctaaa tcataaatgt ttgtatctcg aaactcatta actttatatg ttgatatgtg
    17641 taaataaagt cccttaccat aatcaccaat ataatttgga tcgtgataat ccatataatc
    17701 gttaagtaat aactcttggt aatcatcctc taaattaccc ttaaaaaact tatcgtataa
    17761 ccaatgttct ttattccaag cattaaataa aaatgtaatt tgaaaaaaat aaccttttgg
    17821 taatttgcca cgaatagatc catctaactt tctaaactca tcataatctt taatttcaaa
    17881 tgcttcttca atgtaaacat ctgtaaaaaa accaacggtg gctttcaagc tcgttaattt
    17941 atctgcatta tcaaaaccac gaaaaacaat tacttgtccg gttggaatat aagttattat
    18001 tttatctaca ctattgacct taaaaaatct gcttaatgat atttcgggat ttctaaaatc
    18061 tggctggtta ataagcgata gtattgtatt ccaggtggta attttatggc tattctctgt
    18121 ttgtctaata attagtatat ttcttaaagg ttgagtaagt attttgttta tactctcttg
    18181 acctaacata ttataagttt tctttgagtt tcgagctcct ttatataaac gatatcttgc
    18241 tttacaatta gtccaccaac cattgttata tcctttacca ataatctttt gcattgataa
    18301 agtataagaa ttattatcat tactattact acttgtcttt aacatcgttg ataattttaa
    18361 ttcctaattc atcagcattt atactgatct cttgtttatc accatagttt ttagcatctc
    18421 gcattttcaa aagagcatgt gaagctctcc aatcttgcaa tccagcattc attatatttt
    18481 gcaagtttac ttcaataaaa ctactatacg cattattata taattccata aattgtgaat
    18541 atatggtatc tttccctttt tctaaatctt gattaccttt ccacttataa tcttgaatag
    18601 tcgcaactgc aattccacta cggcgacaag catctgcatc actcatccca agtgatacgg
    18661 ctttcaaaaa taatttaact ttttttggat gagctactaa taatgcttta ttacctcttt
    18721 tatcaacttt ctttttagta gtagtagatt ttttagtagt agtctttttt gctttagtat
    18781 tagtagtggt ttttttacta actttaggtt tattagtagt tttagtttta atagtattat
    18841 ctttctttat ttctttttgt tgttccataa taatatacac tccctttaca taatttttaa
    18901 tatattattt tgtatcc
//
LOCUS       DAOPCH010000012        17708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_3289_length_17708_cov_5.638985, whole genome shotgun sequence.
ACCESSION   DAOPCH010000012 DAOPCH010000000
VERSION     DAOPCH010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 17708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17708
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3289_length_17708_cov_5.638985"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..409)
                     /locus_tag="PLU55_01495"
     CDS             complement(140..409)
                     /locus_tag="PLU55_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86766.1"
                     /translation="MFKTYDEYVQAREAQNRILEVLTKIFKSFPQSPMGGVADEARKT
                     AKYQKVKKEWDIEFKKYQDMNKFASKHFKKEIKADQRKKRSAWRK"
     gene            complement(655..1140)
                     /locus_tag="PLU55_01500"
     CDS             complement(655..1140)
                     /locus_tag="PLU55_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86767.1"
                     /translation="MRTKIISAFPGTGKSMYHQKHRETTLDSDSSNFSWTIDENGNKV
                     RNPEFPKNYIRHIKENIGKYEFIFVSSHKDVREALLDECVFFYLVYPNSARKDEFIER
                     YKNRGNDEQFIKFVSDNWDSWMKEVWFIPDGCKYINMVLNNLEDEIEHLKAVEHGENL
                     A"
     gene            complement(1256..2092)
                     /locus_tag="PLU55_01505"
     CDS             complement(1256..2092)
                     /locus_tag="PLU55_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86768.1"
                     /translation="MSKPLRNFDKFEEGHYYVYTGEKTDKWANAMEAVLERKIHKCLE
                     TPCSNKEGAIFEGVEDGYWNYKDGFDNWVEVESPDAVFEKGDDCLVFRKSSKEWHIAP
                     LINSGFFSCKNMFENTSFVIYILKRKNKKEESVTKNKRYLCPYCKNDRTGFLGLTSKK
                     GESVKCGNCGTKVICVNKGCEDDYINDLVYITSVWNNYEIIKEPTYRPIYTLEEYIEA
                     TKDRKDLRAISKDGTRILSLHEVDANSGEGIIYFSGNSSRYIYENYVWLDDRTLVGVK
                     CS"
     gene            complement(2239..2469)
                     /locus_tag="PLU55_01510"
     CDS             complement(2239..2469)
                     /locus_tag="PLU55_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86769.1"
                     /translation="MTTNNLSHIELKIGDKFRDKNYLYHVVEIIEDGEEAIIVYKYFG
                     KHKRWWHYLVKSKRDINIWFEAGLLRKENKKH"
     gene            complement(2631..2936)
                     /locus_tag="PLU55_01515"
     CDS             complement(2631..2936)
                     /locus_tag="PLU55_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86770.1"
                     /translation="MSEVKRKIDVILDDSQKQYDDAMRPIYEGKAETSFRKAILNAQG
                     LIFDLEKQRAELYLNIEDFDEIDVDTMDDAECAIKDQKDKLERIKAQYKEWFGVDYK"
     gene            complement(2940..3452)
                     /locus_tag="PLU55_01520"
     CDS             complement(2940..3452)
                     /locus_tag="PLU55_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86771.1"
                     /translation="MLRNFEKFEVGHYYVYTGSKTEKTWCPFGGMNFAIEHKILQCCE
                     VHGHTNANFKENERLGGSIWAWKDGFDNWVEVESPEAVFEIGDKVLQYVKNSKTWKER
                     KLSDCLLVNNQRKNGYDTFDCFYYILKRKPKEKDDKILGVDNPFKKYIRQKKYLTFGK
                     KKSILNKKEI"
     gene            complement(3523..3867)
                     /locus_tag="PLU55_01525"
     CDS             complement(3523..3867)
                     /locus_tag="PLU55_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86772.1"
                     /translation="MTTNTELEKEVEELKNFPVKLSWKQIWCGIGIFLTIIGSSFGAG
                     FKVQSEIDKVERAKLESEYIKKIGVLDEKTIELNRQLKESQENCIFFQNRYKFVSEKM
                     KELEKELEEKID"
     gene            complement(4075..4494)
                     /locus_tag="PLU55_01530"
     CDS             complement(4075..4494)
                     /locus_tag="PLU55_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86773.1"
                     /translation="MEIINTIKSIILNKTGAQIKNELGIPQHFTVVIQRIINQPNLIY
                     LLTYNSRVPTMDLTTSRLKQNKAMQLKRPKKSLFGMSLSGMKLTPAVDIDYVKPSITS
                     QSLFILYDMAGEYMRDKSIKENTKNRLNLILYKDLED"
     gene            complement(4593..5042)
                     /locus_tag="PLU55_01535"
     CDS             complement(4593..5042)
                     /locus_tag="PLU55_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HPJ86774.1"
                     /translation="MGVNEVKMMQYKTTTMPLVEWFFSQFDSEDDGGFNHTLPNFPKM
                     NIKSHKEKGWKIEMALAGYSKDEISINLEGGVLTVKGKSEKENDDYTFLRDCRGITSQ
                     NFSQSFRLSEEILDLKEISSKFENGLLTITIPLKAEAIPEKRTIEIS"
     gene            complement(5482..6384)
                     /locus_tag="PLU55_01540"
     CDS             complement(5482..6384)
                     /locus_tag="PLU55_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPJ86775.1"
                     /translation="MIVVGDLHIKNSEPHMKAIGKLFDYLEVNYPNENMMLLGDVFDS
                     TGVPFNVVTYFIERTKKFGKHWYIISGNHDESVLKKNILEPLSHLEHITVFFEKTEID
                     IDGLDCLMLPYVHKAKEVYADIEWQGSVCFLHVTNIEDQWLDEGVNTDKINALQIFGH
                     THTHKEYPNKIILGVTLPTRNGEVSNRILFIDEKTKEHKYIDKDFSEFLSYETIEYGN
                     FPVNKNNILNVIKAPSVPSVHSMYKDYYIREEGIELLIDEAQYEGESVFEFDSADNKK
                     SFVEYGKEKGIRNEIVEKGLEYLV"
     gene            complement(6381..6542)
                     /locus_tag="PLU55_01545"
     CDS             complement(6381..6542)
                     /locus_tag="PLU55_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86776.1"
                     /translation="MKVDLEKLEVCTNCKHLYHNRIIKKECNCGKGKYTSIFEYLENS
                     TYKNKEKTK"
     gene            complement(6553..7563)
                     /locus_tag="PLU55_01550"
     CDS             complement(6553..7563)
                     /locus_tag="PLU55_01550"
                     /inference="COORDINATES: protein motif:HMM:NF021631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPJ86777.1"
                     /translation="MKINANNRDYEGVLQIRTGSHLYGTNTETSDEDFSGIFLPSKYE
                     VFGFETIEEIDNSVVSKDSEGKNTKDAIDFKYYEFRKFCKLALDCNPNILEHLFVNEE
                     NVVYISEVGRELLNLKNYFLGKQSIFTKYSSYAKSQGHKMRLKPENYENLKIVLEYLY
                     NYIGTDTIINMSNKEEQVKSRQLLVELATDINEKKMPIEFKDSFIKIGDLNFNKNINI
                     KNTINSIETRISSAGSRKVLWEQFGFDTKFGANLIRLLLEGIELLTDECLTFPLKEKE
                     LILDIKQGKYTLDEVLKLSEQLSNKLEIAYACSNLPLKSNYKIIQEFVIDTIQSRIDI
                     GR"
     gene            complement(7553..7708)
                     /locus_tag="PLU55_01555"
     CDS             complement(7553..7708)
                     /locus_tag="PLU55_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86778.1"
                     /translation="MKDFNPDYSKCEKSQKQVFHSICDKIRKSTFMFCGDSQIKCIAW
                     EGYKHED"
     gene            complement(7710..9068)
                     /locus_tag="PLU55_01560"
     CDS             complement(7710..9068)
                     /locus_tag="PLU55_01560"
                     /inference="COORDINATES: protein motif:HMM:NF020968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HPJ86779.1"
                     /translation="MKLEIKGSENYSAIIAKIESKIPLDGCDNICGTIIQGSHVIIGK
                     DVKEGDVGIFFPVETQISDFFLRDNNLYEDKSLNSDNTKKGFFNHKGRVRCMRLRGNK
                     SEGFWIPLSTFCNLSKEDIDSFEVGTVFDTIDGITLCKKYVIVKNEKNSSKNKEKQAN
                     KNFNRIIDEQFHFHINTTHLKNNMHCLNFDSNITITQKLHGSSFIVSNILCNKKITWK
                     DKIAQKLGVNVITKDYDNVYASRCVIKNKYINEKVSEGYYGVDLWGVINEELKPFLEK
                     GMTIYGEVVGYLPDSNRMIQKNYDYGCKKGEHKNYIYRITSTNPDGVVFEWSMNQIQM
                     WCKLKGLNAVPLLYSGSVKNLYISLHNNYCESLDGIDFSTFKPDLFLQMITDEYLDIK
                     CDKDNFEVYCNHDVWDEGIVVRVDSLDIECYKHKSFNFLENETKALDAGETNIEDNQE
                     GE"
     gene            complement(9079..9321)
                     /locus_tag="PLU55_01565"
     CDS             complement(9079..9321)
                     /locus_tag="PLU55_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86780.1"
                     /translation="MRKEYYTFLDRTGIRDFFSLIDEIPYSKKLKILEKLNKDYSVGT
                     INKALNEYEINYFRLHRVRLHIIENVLKGLCIKNNL"
     gene            complement(9318..9641)
                     /locus_tag="PLU55_01570"
     CDS             complement(9318..9641)
                     /locus_tag="PLU55_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86781.1"
                     /translation="MKNDKIIKIQQGDIIIKTTVLPKKQWGKKINHLILQKSDVSGHS
                     HKIVGKAVLYSTDKEGEFFLDVLEKSKVLHEEHKTLSVPKGKYFVYGVRELDHFEEEI
                     RRVRD"
     gene            complement(9662..10246)
                     /locus_tag="PLU55_01575"
     CDS             complement(9662..10246)
                     /locus_tag="PLU55_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86782.1"
                     /translation="MFKYYEYYNNVIGFKAKNEKLLNILISISKNSHILFFFKDLVVV
                     IEKPVRIEMEGNQLHSFDKMAVEYSNGKGFYLSHNVKMPEKYIKTSAEKISVNTILKE
                     ENVEVRRELLRKVGIENFIVKSKAKVLDTFEDYTLYKVKLGKDKFGNYLKMKNPSLDN
                     TYHFEGVNNECDTVEKALAWRDSEFDYIKPISLS"
     gene            complement(10213..>10481)
                     /locus_tag="PLU55_01580"
     CDS             complement(10213..>10481)
                     /locus_tag="PLU55_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86783.1"
                     /translation="QLIRQFIRQFIRQFIRQLIRQFIRQFIRQLIRQFIRQFIRQFIR
                     QFIRQFVRQLIRQFIRQFVRQFIRQFIGGNILLICLNIMNITIM"
     assembly_gap    10482..10581
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10582..10893)
                     /locus_tag="PLU55_01585"
     CDS             complement(<10582..10893)
                     /locus_tag="PLU55_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86784.1"
                     /translation="MIERLTKKQIEKMYEYRDEGIKIARDTSLINHSKLEGDIKSLYC
                     ECGLPHDLNFKYFPFFEDFIKDSSIYSSIYSSIYSSIYSSIRSSIDSSIYSSIYSSIY
                     SS"
     gene            complement(10918..11196)
                     /locus_tag="PLU55_01590"
     CDS             complement(10918..11196)
                     /locus_tag="PLU55_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86785.1"
                     /translation="MKTNQDTIIIPKRLSTIPDICFCGVKLEKPKEYLYHYDDEFLQR
                     IVSPRDPHTYYNTNNIFQCYNCGILFINDYDIMGYYVRIKKFKLYKTN"
     gene            complement(11279..12367)
                     /locus_tag="PLU55_01595"
     CDS             complement(11279..12367)
                     /locus_tag="PLU55_01595"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPJ86786.1"
                     /translation="MAENLYSKYRPLTFNEVFGHKTIIKEFKKRAKEDSFNQAILLSS
                     FSGCGKTTISNIIAKTLLCTHKDEDGNPCNVCENCKSVMFEQNTFSYKMLNASNIGID
                     EMRSIEEFADTETFTLNSRKVIVIDELQEISGSKAQKNILKILEKKYKDVYFILLTMN
                     EGKIDTAIKNRCIPYRLKNLTVEEIAEYLCGVCDREAINIDTKEKADVLITIAENSYG
                     SMRTAMSYLERVIYSEIWNKDEVIKELNLISAEDLSNILNMLLSGDINFLSISFEKEI
                     LDRIKYLFVLLYKGLSGIKLNGYENAQVSNIRKVESNILKFALSSIYELNKYSYLTKD
                     LIEFTLINICNFAKDNVTVKAEPIKRRA"
     gene            complement(12377..12943)
                     /locus_tag="PLU55_01600"
     CDS             complement(12377..12943)
                     /locus_tag="PLU55_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86787.1"
                     /translation="MKTNSKSIGNNFERETAKTLSLWLTKGESDDVLWRDLSSGARAT
                     TRKKQNKTTINDGDLVVTDLKYKWFTDCFFIDTKSYKECNFYFINSKNIKSNAILNQW
                     IKVCGDCPKNKIPMMICNIRDRKSTQFVVLPSMFRFIGLNILKLNLITVSFSEELGYN
                     NNDFVIIEFDNFLNNMDAESLCGVNTKK"
     gene            complement(13018..13266)
                     /locus_tag="PLU55_01605"
     CDS             complement(13018..13266)
                     /locus_tag="PLU55_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86788.1"
                     /translation="MYFKDFKCNDCGHVEEVSKARMIDDFIYTECVKCKSKNVSMIFA
                     VGDTDICEGMAGNASTGYKTGIAYHPSKYGRFSGTRIK"
     gene            complement(13268..14686)
                     /locus_tag="PLU55_01610"
     CDS             complement(13268..14686)
                     /locus_tag="PLU55_01610"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HPJ86789.1"
                     /translation="MSELTMFKNTLPSTDKEQEKLIAYLYKNKNEVKEFNSEIFTNAL
                     CHNLYLIFDSFVDNNVPFNIDNIMDSFSDFTYSKEQIENILKSEYLEENVPIIRSKVE
                     EIFSRTKKLYDLERIAEGLINNQKYDEAKIKKYAENILSDTYSVEKDKKLLFADELMT
                     YYKEKYEQRNNGKDIFRSFGFGCIDRLLTRPAGAGETTILEGMKGSAKSLICKSIENS
                     NINKKICVFSINLEMTAESNCDRLMSMRTNLSLSKEILNKELDRRNKERLMKEIERFS
                     KYNNYIYYNSSEMTIDDYDKEIYKAKRMFKSRGVLPEDGYIFVTCDLLDMIEEFSNAS
                     NSYELKHAMNRVHKINMKHGIHLLGLVQANENDIRNGKKFKDPEDCDNYFLQPENIEG
                     GAVYGARARVILALNRPLLLKRRFFPTRSEEWDLEDDIITLAVVKQNDGMVGVSAKFV
                     FDYHSFRLHEYRKQTIENEVGA"
     gene            complement(14696..15679)
                     /locus_tag="PLU55_01615"
     CDS             complement(14696..15679)
                     /locus_tag="PLU55_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86790.1"
                     /translation="MAYKNIAIDCSNLYWRCVCTCLKSAPDKVKEGNTSINVYTSVIQ
                     DFIDRVNNLKKEYLFDNGKVYLLFDNPKSTLTLRQIIDEEYKSPRMHRIIPKIFWDTL
                     KVLKQVLLHYDDNFVFLQYPFCEADDLVKPLIDNICIDENNRLLLVSADMDWSRGISK
                     NIDWFNFNKVYNIKEFYEKYNFKPERKNVVVYKCFRGDASDNIESGLPNFPEKLLLQI
                     LEKNEVNDYHSFREFLKSLQSCDFLSHDWKLKLKEAEERLLKNFQLTNYMCVEASFKE
                     MKKDCKEYISVLKSYYKILQLPFETKMLSEEEYEDSFFQEAKFTRKRKFFR"
     gene            complement(15697..16137)
                     /locus_tag="PLU55_01620"
     CDS             complement(15697..16137)
                     /locus_tag="PLU55_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86791.1"
                     /translation="MKNKELVAKVENITELTNSQKDRLNIQETAHENLCNFLNSHLHK
                     LQSKNELISAIESKFLNSVMGKTDEEIPLIAVIKIYEILKRAETDADSAVLSLFNKNP
                     DIFVNVNQNALDKDEPKLTKDEMNMAKDLLTAIHKLEKSEHSEK"
     gene            complement(16127..16588)
                     /locus_tag="PLU55_01625"
     CDS             complement(16127..16588)
                     /locus_tag="PLU55_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86792.1"
                     /translation="MENIVKEKLKKYLKVEEETASSLFEQLALYIYALEAGKHDLYIL
                     AKLLPEKEISKLVAYFDGDTIKLPTKKEHRTLLLLGVCYYLKEIKRFTWEEIKTFLDL
                     PENNQDLVSSISLGGKINKLNEKISKEWVEMLRNIKVSGLDEFAKGVKNEK"
     gene            complement(16649..17428)
                     /locus_tag="PLU55_01630"
     CDS             complement(16649..17428)
                     /locus_tag="PLU55_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86793.1"
                     /translation="MASGKPITCKYCKNQFKFDSDDDRKKWRDNLYLCPICKIDYCIL
                     PETERELKKLQKLYIDSGKKQKYLNEIAKILYKYVPSLIKRYFTKALYEARRNEGEDA
                     LDNYVHKTVIYVIENYLKRDDFYISVSFAGYCKDAILWSFREKKERQSNDISMDFEMD
                     EEGHTAQYADNKLNLLEKIEQDEYRKDIVKTLMAYIKEMKNFCSQREYYVLLVVLNNY
                     LYKGDKYVDKFFHVYNDNVGKLKYMQFLDILKKEIDCLFFE"
     gene            complement(17496..>17708)
                     /locus_tag="PLU55_01635"
     CDS             complement(17496..>17708)
                     /locus_tag="PLU55_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86794.1"
                     /translation="KYIFKRRLSIMAKPTEVRLNKKEQKTVLSRVKAGVSNAKTIAKE
                     LGKSRYDIMFFLETQGLKKYSEGSYL"
BASE COUNT         5656 a         3112 c         1849 g         6991 t
ORIGIN      
        1 ataaaagggc ggcttacgcc acccctttgc tcctgaaatt tgcctgaaca atttctcttt
       61 cattgttatc cataatcttt tttactcctt aatttatatt taatacctat aacactatag
      121 gtattatttt gtcaactaat tattttctcc atgcacttct ttttttacgc tggtctgctt
      181 ttatttcttt cttaaaatgt ttactagcaa acttattcat atcctgatat tttttaaatt
      241 ctatatccca ttcttttttt actttctgat actttgctgt ttttcttgct tcatcagcta
      301 ctccccccat aggactttga ggaaaagact tgaatatttt tgtcaaaact tctaatattc
      361 tattttgtgc ttcccttgct tgtacatatt catcataagt tttaaacata ttattcctct
      421 tagtcaactt cgatggagca tctaccttct acacttagaa tccattcacc tgtaatacca
      481 ttgtaaaata ctacacgtct aatgatacgg aaattgtctg catcctgaga cagattgtaa
      541 gacgctactg aagcatctga ttgacagttt gttataccta ttatagatat aaccattaga
      601 agcattaacc atagtctttt cataattctt ttattctcct tttaattttt taattcaagc
      661 cagattttcc ccatgttcaa cagcttttaa atgctctatt tcatcttcaa gattatttaa
      721 aaccatgttg atatatttac agccatcggg aatgaaccat acttctttca tccaactatc
      781 ccaattatct gaaacaaatt ttatgaattg ctcatcatta cctctatttt tatatcgttc
      841 aataaattca tcttttcttg cactattagg gtaaactaag taaaagaaaa cacattcatc
      901 taaaagtgct tcacggacat ctttgtgaga agaaacaaat ataaattcat acttacctat
      961 attttctttg atatgtctaa tataattttt tggaaattct ggatttctca ctttgtttcc
     1021 attttcatca atagtccaac taaaattaga tgaatcgcta tccaaggttg tttccctatg
     1081 tttttgatgg tacatacttt ttcctgtacc agggaatgct gaaataatct ttgttctcat
     1141 tttgtaacct ttcttattat taaataaatt actcttcaaa attatcatca ttatcacagt
     1201 actctaacag tttagtagca ataatatgta tgtttattgc ttaccttaca atatgtcaag
     1261 aacattttac acctactaga gttctatcat caagccagac ataattttca taaatatatc
     1321 tacttgaatt accactaaaa tatattattc cttcacctga attagcatca acttcatgca
     1381 aagagagtat tcttgtacca tctttactta tagctcttaa gtcttttctg tcttttgtcg
     1441 cttcgatata ttcctcaagt gtatagattg gtctgtatgt aggttctttg attatttcgt
     1501 aattattcca tacacttgtt atatatacta aatcgtttat gtagtcatct tcgcaacctt
     1561 tattgacaca tatcactttt gtgccacaat taccacattt aacagattcc cccttttttg
     1621 atgttaaacc tagaaaacct gttctatcat ttttacagta aggacataaa tatctcttat
     1681 tttttgttac agactcttcc ttcttatttt ttctttttag aatgtagatt acgaatgaag
     1741 tattctcaaa catattttta catgaaaaaa aaccggaatt gattaaaggg gctatatgcc
     1801 attccttgga agatttacgg aaaacaagac aatcatcacc tttttcaaat acagcatcag
     1861 gactttcaac ttccacccaa ttgtcaaatc catccttata attccaatac ccatcttcaa
     1921 caccttcaaa tattgctcct tccttattag aacaaggggt ttcaagacac ttgtgtattt
     1981 tacgttctaa aacagcttcc atcgcattag cccatttatc tgttttctct ccggtgtaca
     2041 cataataatg accttcttca aacttatcaa aatttcttag tggtttactc ataacactta
     2101 ttactccttg tataatattc acacctaatt taactatctg acaataatat tagtatgcca
     2161 actctttaaa acccaaatct ttaatttttt ttcaaagttc ttttacccat aaagtattca
     2221 aatttgtata aaatatgttt aatgcttttt gttttctttt ctaagcaatc ctgcttcaaa
     2281 ccaaatattt atatctctct tactttttac aagataatgc caccatcgct tatgttttcc
     2341 aaaatattta taaacaataa tagcttcttc accatcttct attatttcta ctacatgata
     2401 caaatagttt ttatctctga atttatctcc tatttttaat tctatatgtg agagattatt
     2461 tgttgtcatt gtttgtttta tattccttta taatttcata tatttttgtt gccatttctt
     2521 ttcgctgttg tacattctct gcaattaacc acataaaata ataactattt gggacaccac
     2581 atggctctaa aggtatcagt atatttcaac tgataccttt ttgtaatggc ttacttgtaa
     2641 tctacaccaa accattcttt gtactgtgct ttaattcttt ccagtttgtc tttctggtct
     2701 ttaatagcac attcagcatc atccattgta tcaacatcaa tttcatcaaa gtcttcaatg
     2761 ttaagataca actcagctct ctgtttttca aggtcaaaga taagcccctg agcatttaaa
     2821 attgcctttc ggaatgatgt ttcggctttc ccttcgtaaa tagggcgcat tgcatcatca
     2881 tactgtttct gagagtcatc caaaatcaca tcaattttac gttttacttc tgacatatac
     2941 tatatctcct ttttatttag tatgcttttt ttcttaccaa atgtcaagta ttttttttga
     3001 cgaatatatt ttttaaatgg attatctact cccaaaattt tgtcgtcctt ctcttttggt
     3061 tttcttttta gaatataata aaaacaatca aaagtatcat acccattttt tctttggtta
     3121 tttactaaaa gacaatcact taatttacgt tctttccatg ttttactatt ttttacatat
     3181 tgtagtactt tatctcctat ctcaaatact gcttcaggtg attctacttc cacccaatta
     3241 tcaaatccat ctttccaagc ccaaatacta ccacctaatc tttcattttc tttaaaatta
     3301 gcattagtat gtccatgaac ttcacaacac tgtagtattt tatgttctat ggcaaaattc
     3361 atgcccccaa atggacacca ggttttttct gttttacttc cggtatatac ataataatgc
     3421 ccaacttcaa atttttcaaa atttcttaac attttaattc actccttaat catcaactta
     3481 tataaccgtt aaatactact ctttgaaaaa gtcaacactt ttttaatcaa ttttttcttc
     3541 taattccttt tctaattctt tcattttttc agatacaaat ttatatctat tctgaaaaaa
     3601 tatacaattc tcttgactct cttttaactg tcgattaagt tctattgttt tttcatcaag
     3661 aacacctatc ttttttatgt actctgattc aagttttgca cgttctactt tatctatttc
     3721 actttgtact ttgaaccctg caccaaaaga tgaacctata atggtcaaaa atattcctat
     3781 gccacaccaa atttgtttcc aactcaattt gacaggaaaa ttctttagct cttccacttc
     3841 tttttctaat tctgtatttg ttgtcatcct tcctcttaaa attaaagtac tttaacttta
     3901 tttagctgtt tatacaaatc cacaaaattg tagaaatgct ttttcatatc ttctttagta
     3961 aatttaacta acggtgtttc tattacaagt attattccat atacaaataa aaatataaaa
     4021 gtaaatagat agtagagaga aagcaaaaca aaagcaatta gactgtgtat catattaatc
     4081 ctccaaatcc ttatataaaa ttaaatttaa tctatttttt gtattttcct tgattgattt
     4141 atctctcata tattctcctg ccatatcata tagaataaaa agagattgac ttgtaataga
     4201 aggtttaaca taatctatat ccaccgccgg tgtaagcttc ataccactta aagacattcc
     4261 aaataaactt ttctttggtc ttttaagttg catagctttg ttttgtttta acctacttgt
     4321 ggtcaaatcc attgtaggta ctcttgaatt gtaggttaaa agatatatta aattaggttg
     4381 atttattatt ctctgaatta ccactgtaaa atgttggggg atgcccaatt cattttttat
     4441 ttgagcacct gttttattta atataatgct tttaattgta tttattattt ccatgctata
     4501 ttagtattcc tctaaagata aaacaaaaag agggggttaa ccagttgaaa tgattaaccc
     4561 ccttaacaaa atattttaag aggtattgat tattagctta tttctatagt tcttttttca
     4621 ggaattgctt cagctttcaa aggtattgtt attgtcaata aaccattttc aaactttgaa
     4681 gatatttctt tcaaatcaag tatttcttcc gaaagtctaa aagactgtga aaaattctgt
     4741 gaggttattc ccctacagtc acgaagaaaa gtataatcat cattttcctt ttcactttta
     4801 cctttaacag taagaactcc tccttcaaga ttaattgaaa tttcatcttt tgaataccct
     4861 gcaagagcca tttcaatttt ccatcctttt tccttatgac tcttaatatt cattttagga
     4921 aagtttggaa gagtgtgatt aaacccccca tcatcttcag aatcaaattg actgaagaac
     4981 cattccacta aaggcattgt tgttgttttg tactgcatca ttttaacctc gtttaccccc
     5041 atttagggca gtataaattt aaaatttatt tttaaaaaac aaactccaag cggacatttg
     5101 tttttaaaaa ccataatatt agtatatcaa gcaagaatat ctcttaaagt attaaaatat
     5161 ctaccatccg tatgttcttt aatctttgta gaaaatttag catcatctat tgagagcatt
     5221 ttttctgtta cactatcaag ggcagtagtg aactacccaa gtctaaagac ttgggcttcc
     5281 tgcttcagtg aaatctgctt tctaaaatca taagatttta gaaagtctga taatttctcc
     5341 acaggcattg tattggtatg ttccatacca atttgtttta aaccttctag tcttatattg
     5401 cttgcactat gagtatctct atctttttgg taaaaattac agcaggaaaa acaacacatt
     5461 taaaaaagct ttctatgata atcatactaa atactccaac cccttttcta ctatttcgtt
     5521 tcttatgcct ttctctttac catattcaac aaatgatttc ttattatctg ctgaatcaaa
     5581 ttcaaataca ctttcacctt catattgagc ttcatcaatt aaaagttcaa taccttcttc
     5641 ccttatgtaa taatctttat acattgaatg tactgaagga actgaaggtg cttttataac
     5701 atttagtata ttatttttat ttacaggaaa attaccatac tctattgttt cataggataa
     5761 aaactctgaa aagtccttat caatatattt atgttctttt gttttttcat ctataaacaa
     5821 aattctatta gatacctctc cgtttcgggt tggaagtgta acccccaaaa ttattttatt
     5881 tggatattct ttatgtgtat gagtatgacc aaatatttga agagcattta tcttatcagt
     5941 attaaccccc tcgtctaacc attgatcttc aatattggtt acatgtaaaa aacaaaccga
     6001 gccttgccat tcaatatcag catacacttc ttttgcctta tgaacataag gaagcattaa
     6061 acaatcaaga ccatcaatat ctatttcagt cttctcaaaa aatactgtta tatgttctaa
     6121 gtgtgataaa ggttctaata tgtttttctt caagacactt tcatcatggt ttcctgatat
     6181 tatataccaa tgcttaccaa acttttttgt tctttctata aaataagtaa caacgttaaa
     6241 gggaacacct gtactatcaa acacatcccc aagcaacatc atattttcat taggataatt
     6301 tacttctaaa taatcaaata gttttcctat tgctttcatg tgtggttctg aatttttaat
     6361 gtgcaagtca ccaactacta tcatttcgtt ttctccttat tcttataggt actgttctca
     6421 agatactcaa aaatagaagt atatttgccc ttaccacaat tgcattcctt tttgattatt
     6481 cgattatgat ataaatgctt acaattagta cacacttcta atttttccaa atcaactttc
     6541 atttttattc tcctatctac ctatatcaat tcttgattgt attgtgtcaa taacaaattc
     6601 ttgaattatt ttataatttg attttaaagg caaattagaa caagcataag caatttccaa
     6661 tttatttgac agttgctctg atagtttcaa gacttcatct aaggtatatt ttccttgttt
     6721 aatgtcaaga attaattctt tttcttttaa agggaaagtt aagcattcat cagttagtaa
     6781 ttcaattcct tctaaaagaa gtctaattaa gttagcccca aattttgtat caaatccaaa
     6841 ctgttcccat agaacttttc tacttccagc actactaatt cttgtttcaa tagagttaat
     6901 tgtattttta atatttatat ttttattaaa atttaaatcc cctattttta taaatgaatc
     6961 tttaaactca ataggcattt tcttttcatt aatatccgta gcaagttcaa caagtaattg
     7021 ccgtgattta acctgttctt ctttatttga catattaata attgtgtctg ttcctatata
     7081 attatataaa tattctaaaa caatttttag attttcataa ttttcaggct ttaatctcat
     7141 tttatgtcct tgtgatttag cataggaaga atatttagta aatatgcttt gtttacctaa
     7201 aaaataattt ttaagattaa gaagctctct acctacctcc gaaatatata caacattttc
     7261 ttcattaaca aagaggtgtt ctaaaatatt aggattacag tccaaagcaa gtttacaaaa
     7321 ttttctaaat tcatagtatt taaaatcaat agcatctttt gtatttttac cctctgaatc
     7381 tttagaaaca acactgttgt ctatttcttc tatagtctca aatccaaaaa cttcatattt
     7441 ggatggtaaa aatattcccg aaaaatcttc atcacttgtt tctgtatttg taccatacaa
     7501 atgactacct gttcttattt gaagtacacc ttcataatct ctattattag cattaatctt
     7561 catgtttata cccttcccat gctatacatt taatttgaga atccccacaa aacataaaag
     7621 tagactttct tattttatca caaatagagt gaaaaacttg tttttgtgat ttttcacatt
     7681 ttgaataatc aggattgaag tctttcaaat tattctcctt cctgattatc ttcaatgttc
     7741 gtttcccctg catctaaagc ttttgtttcg ttttccaaga aattaaacga tttgtgtttg
     7801 tagcattcaa tatctaaaga atcaacacgt actacaattc cttcatccca tacatcatgg
     7861 ttacaatata cttcaaaatt atctttatca cattttatgt caagatattc atcagtaatc
     7921 atttgcaaaa acaaatcagg tttaaatgta gaaaagtcta taccatcaag agactcacaa
     7981 taattattgt gtagggatat atataagttt ttaacacttc cagaataaag taaaggtaca
     8041 gcattcaatc ctttaagttt acaccacatc tgtatttgat tcattgacca ttcaaacact
     8101 acaccatcgg ggtttgttga tgttattcga tatatataat ttttatgttc tcctttttta
     8161 caaccataat cataattctt ttgaatcatt ctgtttgaat ccggcaaata tccaactact
     8221 tctccatata tggtcatacc tttttcaagg aaaggtttta attcttcatt gataacaccc
     8281 cataaatcca caccataata tccttcagat actttttcat ttatatattt attttttatt
     8341 acacatcttg aagcatacac attatcataa tcttttgtta taacatttac accaagcttc
     8401 tgtgcaattt tgtccttcca tgtaatcttc ttattgcaaa gaatatttga tacaataaat
     8461 gaactaccat gaagcttttg agttatagtt atattagaat caaaattcaa acaatgcata
     8521 ttatttttca aatgtgttgt gttaatatga aagtgaaact gttcatctat tattctatta
     8581 aaatttttat ttgcttgttt ctctttattt tttgatgaat ttttttcatt tttaactata
     8641 acatattttt tacacaaagt tataccgtca atagtatcaa atacagtacc aacttcaaaa
     8701 gagtcaatat cttctttact taaattacag aatgttgaaa gtggaatcca aaaaccctcc
     8761 gatttattcc ctctaagacg catacatcta acacgtcctt tatgattgaa aaatcctttc
     8821 ttggtattat cagaatttaa agacttatct tcatataaat tattatctct caaaaagaaa
     8881 tctgatattt gagtttcaac tggaaaaaat attccaacat ccccctcttt tacatccttt
     8941 cctataataa catgactacc ctgaatgatt gtaccacaaa tgttatcaca accatcaaga
     9001 ggtattttac tctcaatttt tgctataata gctgaataat tttctgaacc tttaatttct
     9061 aatttcattt tatttctcct ataaattgtt tttaatacaa agtcctttta aaacattttc
     9121 aattatatga agtcttactc tatgcaatct aaaatagttt atttcatatt cattcaatgc
     9181 tttattgata gtaccaacac tataatcttt atttaatttt tcaagtattt ttaatttttt
     9241 agaataagga atttcatcta taagactgaa aaaatccctt attccggttc tatctaaaaa
     9301 agtgtaatac tcttttctca atcccttact cttcttattt cttcttcaaa atggtcaagc
     9361 tctcttacac catatacaaa gtattttcct ttaggaacag aaagtgtttt atgctcttca
     9421 tgtagtacct tacttttttc taaaacatca agaaaaaact caccctcttt atctgttgaa
     9481 taaagaaccg ctttcccgac aattttgtga gaatgtcctg aaacatcaga tttctgaaga
     9541 ataaggtgat taatcttttt gccccattgt tttttaggaa gaacagttgt ctttatgatt
     9601 atatctcctt gctgaatttt aattatttta tcatttttca ttttatttct ccttattatt
     9661 tttagcttaa actaattggt ttaatataat caaattctga atctctccaa gccaacgctt
     9721 tttctacagt atcacattca ttattaacac cttcaaaatg ataagtattg tctaatgaag
     9781 gatttttcat ttttaaataa ttcccaaatt tatcttttcc aagcttcact ttatataaag
     9841 tgtagtcttc aaaagtatca agaactttag ccttgctttt aacaataaag ttttcaatcc
     9901 caacttttct taaaagttct ctcctaactt ccacattttc ttctttaaga attgtgttta
     9961 cagatatttt ttctgctgat gttttaatat atttttcagg cattttaaca ttatgactta
    10021 aataaaatcc ttttccatta gaatattcaa ctgccatttt atcaaatgag tgcaattggt
    10081 ttccttccat ttcaattcta accggttttt caataacaac caccaaatct ttaaaaaaga
    10141 aaagaatatg agagttctta gaaatagaaa ttaaaatatt taataatttt tcattttttg
    10201 ctttaaaccc tattacatta ttgtaatatt cataatattt aaacaaatca atagtatatt
    10261 gcccccaata aattgacgaa taaattgacg aacgaattga cgaataaatt gacgaatcaa
    10321 ttgacgaacg aattgacgaa taaattgacg aataaattga cgaataaatt gacgaataaa
    10381 ttgacgaatc aattgacgaa taaattgacg aataaattga cgaatcaatt gacgaataaa
    10441 ttgacgaata aattgacgaa taaattgacg aatcaattga cnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn nnnnnnnnnn ntgacgaata aattgacgaa taaattgacg aataaattga
    10621 cgaatcaatt gacgaacgaa ttgacgaata aattgacgaa taaattgacg aataaattga
    10681 cgaataaatt gacgaatctt ttataaaatc ttcaaaaaaa ggaaagtact taaaattcaa
    10741 atcatggggt aagccacatt cacaatataa tgactttata tcaccttcta attttgaatg
    10801 attaattaag gaagtatccc ttgcaatttt aataccctca tcccgatact catacatttt
    10861 ttcaatttgt ttttttgtta atctttcaat cattttattt ttctcctatt ttcttcttca
    10921 atttgtttta tataacttaa atttttttat tctaacataa taccccatta tatcataatc
    10981 attaataaat aaaattccac aattataaca ttggaatata ttgtttgtat tataatatgt
    11041 atgtgggtct cttggggata caattctttg taaaaattca tcatcatagt gatataaata
    11101 ttcctttggt ttttctaatt ttaccccaca aaaacatata tcaggaattg tagatagtct
    11161 ctttggaata attatagtat cttggtttgt tttcatcaaa aaaatccttt ttattctatc
    11221 ttcattttct cctacatata accattaaac tcccctatta aaaaatgtca agactttttt
    11281 atgcccgtct tttaattggt tctgctttaa cagttacatt atctttagca aagttacata
    11341 tattaattaa agtaaattca attaagtcct ttgttaaata actatattta ttcaattcat
    11401 atatagaaga taaagcaaat tttaatatgt tactttccac tttcctaata ttgcttactt
    11461 gtgcattttc atatccattt agttttattc cactaagtcc tttgtatagc agaacaaata
    11521 agtattttat tctatcaagt atttcttttt caaaagaaat tgaaaggaag ttaatgtccc
    11581 ctgataaaag catattaagt atattactta aatcttctgc acttatcaaa ttaagttctt
    11641 ttataacttc atccttattc caaatttcag aatagataac acgctctaaa tatgacatag
    11701 ctgttcgcat actaccataa ctattttctg ctatagtgat aagtacatca gctttttctt
    11761 ttgtatcaat attaattgct tctctatcac acacaccaca taaatattct gcaatctctt
    11821 ctactgttaa atttttaagt ctgtaaggaa tacatctgtt tttaatagca gtgtcaattt
    11881 ttccttcatt catagtcaaa agaataaaat aaacatcctt atatttcttc tccagaattt
    11941 taagtatatt tttctgtgct ttactaccgg atatttcttg aagttcatct attactataa
    12001 cttttctgct attaagagtg aatgtttcag tgtcagcaaa ttcttcaatg cttctcattt
    12061 catctatacc aatgtttgaa gcatttaaca ttttataaga aaatgtattc tgttcaaaca
    12121 taactgactt acaattttca caaacattac aagggtttcc atcttcatcc ttatgtgtac
    12181 aaagtaaagt tttagcaata atgtttgata ttgtagtttt accacatccc gaaaaagaac
    12241 ttaaaagaat agcctgatta aaactatctt cttttgctct ttttttaaat tccttaataa
    12301 ttgttttgtg tccaaacacc tcattaaaag ttaaaggacg atatttgcta taaagatttt
    12361 ctgccatttg tttctcctac ttttttgtat taacaccaca tagtgattca gcatccatat
    12421 tgttaagaaa attgtcaaac tctattataa caaaatcgtt attgttataa ccaagttctt
    12481 cactaaaaga aactgtaatt aaatttaatt ttaatatatt taaacctatg aatctgaaca
    12541 tagaaggaag tactacaaat tgagtagact ttctatccct aatattacaa atcatcatag
    12601 gtattttatt tttaggacaa tcaccacaaa cttttatcca ctgattaaga attgcatttg
    12661 attttatatt ttttgaattt ataaaataaa aattacactc cttatatgat tttgtatcaa
    12721 taaaaaaaca gtcggtaaac catttatatt tcaaatctgt tacaaccaaa tcaccatcat
    12781 ttattgttgt tttattctgt ttttttcttg ttgttgccct tgctcctgaa gaaaggtcac
    12841 gccacaaaac atcatctgat tctcctttag tcaaccacaa agaaagagtt tttgctgttt
    12901 cacgttcaaa attatttccg atagatttag agtttgtttt catctaagcc accttaacga
    12961 tatttaattt tttgtttaaa tttagaaaat atatatcttg taaattcttt cttcatgtca
    13021 cttaatcctt gttcctgaaa atcttccata cttagaaggg tggtaagcaa ttcctgtttt
    13081 ataccctgta cttgcgtttc ctgccatacc ttcacatata tctgtgtcac ctactgcaaa
    13141 aatcattgaa acattttttg atttacattt aacacattca gtgtaaataa aatcatcaat
    13201 cattcttgcc tttgatactt cttctacatg tccacaatca ttacatttaa aatctttaaa
    13261 atacatacta agcacccacc tcattttcta tagtttgttt tctatattca tgcagtctaa
    13321 aactatgata gtcaaataca aatttagctg aaacacctac cataccatca ttttgtttta
    13381 caactgcaag agttataata tcatcttcta aatcccattc ttcacttcgg gtaggaaaaa
    13441 atcttctttt tagtaacaaa ggtctattaa gtgctaaaat tactcttgct cttgctccat
    13501 aaacagcacc accttcaata ttttcaggct gaagaaaata attatcacaa tcttcagggt
    13561 ctttaaattt tttaccattt cttatatcat tttcatttgc ctgaaccaac cccaataaat
    13621 gaataccatg tttcatattt attttatgta ctcgattcat tgcatgtttt aattcataag
    13681 aatttgaagc atttgaaaat tcttcaatca tatctagtaa atcacatgtt acaaaaatat
    13741 aaccatcttc aggaagaaca cctcgactct taaacatcct ttttgcttta taaatttctt
    13801 tatcataatc atcaatagtc atttctgaag aattataata aatataatta ttatatttag
    13861 aaaatctttc aatctccttc atcaatcttt ctttgtttct tctatccaat tctttattta
    13921 aaatctcttt actaagagaa agatttgtcc gcatagacat caatctatca caattagatt
    13981 ctgctgtcat ttcaagatta atggagaaaa cacatatctt tttatttata tttgaatttt
    14041 caatagattt acatatcaag ctttttgcac ttcccttcat tccttcaagt attgttgttt
    14101 ctcctgctcc tgctggtctt gtaagtagtc tatcaataca tccaaaacca aaggaacgaa
    14161 aaatatcttt tccattatta cgctgttcat acttctcttt atagtatgtc atcaactcat
    14221 cagcaaacaa taacttttta tctttttcaa ctgagtaagt gtcacttaaa atattttcag
    14281 catatttttt tattttcgct tcatcatatt tttggttgtt tattaatcct tcagcaatcc
    14341 tttctaaatc ataaagcttt ttagttctac taaaaatctc ttcaacttta cttcttataa
    14401 tcggtacatt ttcttctaaa tattctgatt taagtatgtt ttcaatttgt tctttagaat
    14461 aggtaaaatc agaaaaacta tccataatgt tgtcgatatt gaaaggaaca ttattatcaa
    14521 caaagctatc aaatataaga taaaggttat ggcacaaggc gtttgtaaat atttccgaat
    14581 taaattcttt aacttcattt ttatttttat ataagtatgc aattagtttc tcctgttcct
    14641 tatcagtaga aggtagtgta ttcttaaaca tggtgagttc tgacatttat ttctcctatc
    14701 taaaaaactt tctttttctt gtaaacttag cttcctgaaa aaaagaatcc tcatattctt
    14761 cttctgacaa cattttagtt tcaaaaggta attgtaatat cttataataa ctttttaata
    14821 cacttatata ctccttacaa tcttttttca tttctttgaa tgaagcttca acacacatat
    14881 aattagtaag ctgaaaattt ttcaataatc tttcttcagc ctctttaagt ttaagtttcc
    14941 aatcatgact taaaaaatca caagactgta aacttttcaa aaattcacga aaagaatgat
    15001 agtcatttac ttcatttttt tctaatattt gtagtaacaa tttttctgga aaattaggta
    15061 aaccactttc aatattatct gaggcatccc cacgaaagca tttatatact actacatttt
    15121 ttctttctgg tttaaaatta tatttttcat aaaattcttt aatattatat accttattaa
    15181 agttaaacca atcaatattc ttggaaatac ctcttgacca atccatatct gcacttacaa
    15241 gaagtagtct gttattttca tctatacaaa tattatcaat caaaggtttt acaaggtcat
    15301 ccgcttcaca gaaaggatat tgaagaaaaa caaaattatc atcataatgt aataaaactt
    15361 gtttgagaac tttaagagtg tcccaaaaaa tctttggtat aatcctgtgc attcttggag
    15421 atttatactc ttcatcaatt atctgtctaa gtgttaaagt acttttagga ttatcaaata
    15481 gtaaataaac tttaccatta tcaaaaagat attctttttt taaattgttt actctatcaa
    15541 taaaatcttg aataactgag gtatatacat tgatagaagt gttgccttct tttactttat
    15601 caggtgcact tttaagacaa gtacacacac atctccaata taaatttgaa cagtctattg
    15661 caatgttttt atatgccaat gttaaccttc ccttttctat ttctctgagt gttcagattt
    15721 ttctaattta tgtatagctg tcaacaaatc ttttgccata ttcatttcat ctttagtcaa
    15781 tttaggttca tctttgtcca gtgcattttg attaacattg acaaatatat cgggattttt
    15841 gttgaacaat gaaagtactg cactgtctgc atctgtctca gctcttttaa gaatttcata
    15901 tattttaatt acagctatta atggtatttc ttcatctgtt tttcccataa cactattcaa
    15961 aaattttgac tcaatagcag aaatcaattc atttttggat tgaagtttat gtaaatggct
    16021 attcaaaaaa ttacacaaat tctcatgtgc tgtctcttgg atgtttaatc tatctttttg
    16081 agagtttgta agctctgtta tattttctac ttttgcaaca agttctttat ttttcatttt
    16141 taacaccttt agcaaattcg tctaatcctg aaactttaat atttcttaac atttcaaccc
    16201 attctttaga tattttttca tttaatttgt ttatttttcc acccaaagaa attgacgaaa
    16261 caaggtcttg attgttttct ggtaagtcaa gaaaagtttt tatttcttcc caagtaaatc
    16321 ttttaatttc tttcagataa taacaaacac caagcaacaa tagagttcta tgttcttttt
    16381 ttgttggaag ttttattgta tcaccatcaa aatatgctac caatttactt atctcctttt
    16441 cgggaagcaa tttagcaaga atatacaaat catgtttccc tgcttccaat gcatatatgt
    16501 ataatgctaa ttgctcaaac aaactagagg ctgtttcctc ttctactttt aaatactttt
    16561 taagtttctc tttaactata ttttccattt aatttttcct cttatgtact ctatacttag
    16621 tacacaaaga aaaaacttat gaaagttttc attcaaaaaa caaacaatca atctcctttt
    16681 ttaaaatatc aagaaactgc atatatttta acttacctac attatcattg taaacatgaa
    16741 aaaacttgtc aacatattta tcacctttat acaaatagtt attaagaact acaagtaata
    16801 cataatattc tctttgtgaa caaaagtttt tcatttcttt tatataagcc atcaaagttt
    16861 ttactatatc ttttctatat tcatcttgtt ctattttttc aagcaaattt agtttattgt
    16921 cagcatactg ggctgtgtgt ccttcttcat ccatttcaaa atccattgaa atatcatttg
    16981 actgtctttc ttttttttcc cgaaacgacc aaagaatagc atctttacaa tagccagcaa
    17041 atgaaactga aatataaaag tcatcacgtt ttaaataatt ttctataaca taaattactg
    17101 tcttgtgtac ataattatca agagcatcct caccttcatt tcttcttgct tcatataaag
    17161 cttttgtaaa atatctttta attaatgaag gaacatactt atatagtatt ttagcaattt
    17221 catttagata cttttgtttt tttccactat ctatatacaa tttctgtaac ttttttaatt
    17281 ctctttcagt ctcaggaaga atacaataat caattttaca tataggacat agatataaat
    17341 tatcacgcca ttttttacgg tcatcatctg aatcaaattt aaattgattc ttgcagtatt
    17401 tacaagttat tggtttccct gaagccattt atttctcctc aaattgaaaa aataaacacc
    17461 ctcttttcag aaggtgtttg tgaaactaaa aaatattaaa ggtaagaacc ttcagaatac
    17521 tttttaagcc cctgtgtttc aagaaaaaac ataatgtcat atcttgattt tccaagttcc
    17581 tttgcaattg ttttagcatt agaaactcct gccttaactc ttgaaagaac agttttttgt
    17641 tctttcttgt tcaatcttac ttctgttggt tttgccatta ttgacaatct ccttttaaaa
    17701 atatattt
//
LOCUS       DAOPCH010000013        16822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_3475_length_16822_cov_5.554721, whole genome shotgun sequence.
ACCESSION   DAOPCH010000013 DAOPCH010000000
VERSION     DAOPCH010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 16822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16822
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3475_length_16822_cov_5.554721"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="PLU55_01640"
     CDS             complement(<1..1333)
                     /locus_tag="PLU55_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86795.1"
                     /translation="MGANNYGNANITFRYKHPAKAENFNSVLRGVCPSGIYKGGTVSY
                     IGNILTLAPYTAIFESSNDKATTITTTSSIDLSTVTTFGGNASSATPYVIMRYTYSNV
                     ISNYPDYYQVAFEDIQSTDIIICKVLFDGGGVVNGIDYSEAHTAPQYNKDLNTFNIAC
                     DTFIKNKKINKDITTGIDVNTTTNELICNTSLFFTPTADRTLDITAGAHFADIDLYIY
                     NNSDTYKVTLTYYMSNTFDVLPNGYVALRWNGTRWIIFDSSEMKKEIIRELDANYTAS
                     KFENNTDLFFVPTADRTINLTAGARFVGCEVLISNDASSTYKLTVSFGGVETIDIHFE
                     CKVKFTYSSTGWTYEVLTDFSRETETIIKGTFLGYQKYRKVVNFGALPNAGQKEVAHG
                     IQTPYTIVSISGIAFDGNTDLTLPHTNYGSTAYSIYVFIRHTDHKVAVGTGT"
     gene            complement(1375..2598)
                     /locus_tag="PLU55_01645"
     CDS             complement(1375..2598)
                     /locus_tag="PLU55_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86796.1"
                     /translation="MRNTRWGELLDVVQDLWVQLKQEKVLPIFNQFDMNKATRDEKIE
                     IAKTFGFHLLTFDGYTSTDYFLNKEIQTIVPRIKYKNAIPSYTYTGYVFDLLINLYAI
                     SATNTGGLTTSKLSTIYNPDTQITYAVTELDQEGDNILYYVNFDPWELDCDSEDCDFT
                     LDSDNDLYTLDAQFEFYDDPKETGKEATTLDSDDFISLDPATGEETGGGESIITQITR
                     NLNFNYKHKFVENENEFMSLNTLKVLKNDIDQIHKVTEVIYYTPKLEINVKKDLTPVT
                     QTYSNYNVNLTANQYNILISDDIEDLYYLEFGTGTQSISGTITGVNNFSYRYPDYISG
                     VIPLSGIINYFSETNNSGCIAFDFNIDERNRFTEFTEIAMYNKNSGLVLYSTFPKIQW
                     DENMYNNIQFEICYV"
     gene            complement(2646..4460)
                     /locus_tag="PLU55_01650"
     CDS             complement(2646..4460)
                     /locus_tag="PLU55_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86797.1"
                     /translation="MIWTFDSIKEAVKTKLSLMSQWNTIIYFGVYERILDAIAYVMEK
                     LAYTADILYSNASFIKTNDLKSLNIMSKSFSYTVHRKVGANGVVLVSKDEDMSPAYIY
                     TGKNVYIPKYTQFTDSSGNKKVYAIQDYYYFNGTEGNLEVQVRQGTPKTFFYTASGVA
                     NEEIKIYSPDIDNDIFDVYIVNNDNEILYEVNIVDKMYFVNDTVNYNCSSVSFDDLQG
                     VTFTFGDGISSKKLEANDKVMIKYVETDGANGNIESANTITVIKDTLKDVDDNIITLY
                     CNNVDAISDGADYENIESIRNNAPNLFFAGYRCGTANDWKTILENFSFIYKAVVWSVN
                     DIGGSTLMSEQNKVFATALTNTGDNLTPAQKTYLENQIYYTYKSPTEILQFTNTQKIY
                     IMFNVNAKISNSTTSIVSSQIKDLIYDAYSTLNTNYQTNIYSSNYINDIDDLTYIDYH
                     ETTIKLMDYNVNAIQTDRLVNAFFVSSLPSQEISLVNNSLELWIKRKIGGVWQEPVQI
                     AYSLGTSFIGMNGYTISSSNINYSTGYYNYVIQTIVDNPTQYPIQNPSESQNDGYLIV
                     MAYNTKNGYGLQTQDVRLPYRYQITDIDKDFIFTTLSN"
     gene            complement(4477..5067)
                     /locus_tag="PLU55_01655"
     CDS             complement(4477..5067)
                     /locus_tag="PLU55_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86798.1"
                     /translation="MAINYFDYDIEGNTFIDGNAIVYENDEAIKNAISQWLVSKRGDY
                     LKNPSEGGILDYFLFKNMTNSNLELIGFAIQNLFTINLVSLVKLVNISIQPDYTNRSI
                     DIDVVYKINGTNTVVTEQLSMDKVIDKKSNTYLDIDYIEENLFEFVKIQKYSHPSQKL
                     IYDNDVLSWVWGKYKFINLVDTDEYFERILYVCNVS"
     gene            complement(5083..5754)
                     /locus_tag="PLU55_01660"
     CDS             complement(5083..5754)
                     /locus_tag="PLU55_01660"
                     /inference="COORDINATES: protein motif:HMM:NF016595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HPJ86799.1"
                     /translation="MNGLGFKLHYADVIDNNDSEKKGRIKIKIPYLHKNTTNDLLPWA
                     TQFCIGGGGSNEYGKSFIPEKDSKVWIGFYDEPDMQKPFYIADIQLSEIHPHNLFEDS
                     IKSEIESSSSYPNTKYTYYKNGICIGVDSSDDSPEIFVYHPSGSYFKIDNDGCYLKSG
                     SVAYEKMLKGETVKQFFDSLLTAIQGITVTVTGGLTGGVSNAPSFEILRAQLDTLLSE
                     KNYNN"
     gene            complement(5766..6065)
                     /locus_tag="PLU55_01665"
     CDS             complement(5766..6065)
                     /locus_tag="PLU55_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86800.1"
                     /translation="MRELVYNVLQDDNNVTIYDSRSLNWSTFKFSSKFKRHTLTKDEI
                     LRPYLVSAFYYGTIDYEDILLILNNIEDIFDVIAGTEIIIPDKQEIDDFILANKK"
     gene            complement(6062..6955)
                     /locus_tag="PLU55_01670"
     CDS             complement(6062..6955)
                     /locus_tag="PLU55_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86801.1"
                     /translation="MNRLFVEVLKSNIQPESFLLQEGFTIDAKSEFSSFGELLPGGIT
                     NLVNLAISYSSTGSTVGAGTVYMSNVMDLKRWMKTYPASINCTLKFYTREDVVTDVWL
                     PMNFFLSLGILSRIEEGTGDKKRGSFVTPAPSLGSCGQISRALNGDISTTATPKEKKP
                     LSEFLSSNAKQAGFGLDFSEIKSKILSVLIPGVIYLPYCFVEEVTPTYSNQVVVADNG
                     KEYPLWGEIQLRLSGIYSAIDDMLDDSNKIDIISKDWEKNGLVSSNENAYPTINFESD
                     DATKAKEAQQASHKEEIGETK"
     gene            complement(6952..>7566)
                     /locus_tag="PLU55_01675"
     CDS             complement(6952..>7566)
                     /locus_tag="PLU55_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86802.1"
                     /translation="EVFSKMVITGFKILAVIIKALIAPFRFFVFLIREVVKGFQKLKT
                     GGFTKDMITGAFTSVKDAFKNAFSSVFDTFLGLFDMLIYHISQYIPNMEDRGDAYKVS
                     SAEAYITEQAYIRAYSEKYKVDDDRLHNIAKGKVSEESIKAGAEMDFYKSIKNLKIMG
                     EGQQIAHLKELVKAVREGNMKKETNLTVSTGGTQTEASLKKEKQ"
     assembly_gap    7567..7666
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7667..8405)
                     /locus_tag="PLU55_01680"
     CDS             complement(<7667..8405)
                     /locus_tag="PLU55_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86803.1"
                     /translation="MATVVLPKPTPSPDETLSDIKGFVEDVNDTFKSDKRAQRKADKE
                     QLDVTERMSENMEWFGSMYEQTNTGKKAKIRDKLSEGWTKVFKNKWFEGIFGFMRRMS
                     LGGIGDFLGLLLGIFVLIQTGLLDVLIPILINMAQVLITAIIKYLPRFIKFLIHILKD
                     VLPPFFNTIFNTILDAMGVAKDSPLRKIIKPLSKILPWLLAFGLLIGKFLPMIMTAVR
                     FLQPVISGLMKFLPHIGKLLAGLNPVGI"
     gene            complement(8413..8541)
                     /locus_tag="PLU55_01685"
     CDS             complement(8413..8541)
                     /locus_tag="PLU55_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86804.1"
                     /translation="MKQQESEIMNMFYYVIKKRYSQLVEYQKKVNEEMEKAHKGKK"
     gene            complement(8543..9652)
                     /locus_tag="PLU55_01690"
     CDS             complement(8543..9652)
                     /locus_tag="PLU55_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86805.1"
                     /translation="MSEFIKRRENKPQEVEGVVDKKEIFNTAGTAVVDFETEGRFSLP
                     KQMNFYDFKGKDVSDMTLCKENERLETLVTLLNKNKHEKYKDVDLQEATPEELLEILV
                     NIKANYQGNYFPFYWLCDCQNDVPEKDRVANTDNIELSTLKFASISDADKKLREFYKD
                     MFENPENFSTYLKMKYKTSDIDISKYKIEDELEKIEIKEPFIVADGKNVYEFEFPRIK
                     HMIIANELVEKKYYNVVKNIQTRKEHSVPLAELQRKKENELEKVEREKSKDFIAYVKA
                     LSLLTKNGEPLSNEQKVKELEEMSSSVFVDLTYSLDQIKFGLDDERELSCPLCGKKSK
                     RVLRREIDIQYFLPTNIATTRESRKSTKLNIFIGA"
     gene            complement(9701..10411)
                     /locus_tag="PLU55_01695"
     CDS             complement(9701..10411)
                     /locus_tag="PLU55_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86806.1"
                     /translation="MLPESIVTALDYTRKPFGLINMGIKEIFSLINFQSLNQFEIIFI
                     QKYTKDASKKPQASSTSSTGVASQLFSSVGNVLERVNTSAILDQAVATFLANSITPQF
                     SGIEYERNSHKNYVKELVHPEEISITFIETEMGTVRTWIDAWQDEIFEVTTDGRVRWK
                     NNQEASKKDCWIIPQTRMGLPTFAIKVCGMKFKNIDPPTFAQDEGEPMKLNVIFSVDM
                     CWFLRFEDVKNAVTGIFT"
     gene            complement(10471..10983)
                     /locus_tag="PLU55_01700"
     CDS             complement(10471..10983)
                     /locus_tag="PLU55_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86807.1"
                     /translation="MGIASDLMKLGSDQLQTQFEIIFPKGIPGGGNTDLLSLRADTQF
                     DPPERTVGSYEVFKKGVKMVYTNMVQDMDKSSLTIEFRLDQEWAVYDALNSWHKLCYD
                     YETGTALPEIATRTDIYVIAEDKQLNTVKTLKFIDAKIKSLKVGTFDNQGADPVRVTA
                     IFIFNDMDME"
     gene            complement(11058..12590)
                     /locus_tag="PLU55_01705"
     CDS             complement(11058..12590)
                     /locus_tag="PLU55_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86808.1"
                     /translation="MPYRSKTQERDYSGFVQPNIFEIGAMVIKAPKGKKTPVLCTSEE
                     DILREYGAPSSSYPDIFEAIAFCSVAPCYISAPYHSDALFGGMDVQISGVNGFGTGRN
                     IDTFSFDAFPDVSHAFFATSPYTDNLVGKITYISGKKFKLDLYKSSSVGNELLNSYVY
                     SLNIEKDAFGKSLYIMDVFDNNYYVIPKVNSNFTGTAYNLSGLTANFSGGTRGSEIDS
                     ADILAGWNNFRFANKYPVRIMMDTRGDSAVTVNNLIQTYQPYAQGICAIPMGNNATEA
                     VAFRQGLGIDSDDIGFYTNWAKIKDNYNNSFAWISNIGSVGKKFAKMADVFDSGSPAG
                     IDENGHGGQLADWEVIEVEYDYTDSDFNSELELLDDAQINPIIKDINYGLMIYGDKTS
                     QVTLSDTSYVGTRRVYKYIIKNIAEQVLRLQEFKNNDIPHRRTAKVKTEAILNPVRRE
                     GGITDFKVVCDASNNTADVLDQRQFLLDVYVVAMANSQEVKLRFTRMPQGTINIEISA
                     VS"
     gene            12698..13459
                     /locus_tag="PLU55_01710"
     CDS             12698..13459
                     /locus_tag="PLU55_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86809.1"
                     /translation="MPIFIEDTYLNQIKACLAFPDVDNLLLSDDQIKDICIAPSLREY
                     FSKFPIKHEYQEPVGFNEEKTVSFPDSYVFGVLTCCVSDIGNNVVGSGGDFWTIVAYN
                     SFGMGNINVMRGSGGMYGIRGYNPNNAMEMNEMYRDKWKSYQNKYVTLKFHPDYVKKE
                     IKVYSTANGMLNITWAKHSENFQDVQFQFINDVIDLSKARLLDHLADTSGILSDTNLE
                     ITINAEDLKDKASALRDKVIEKWSAIPDIVYLHVV"
     gene            complement(13456..13800)
                     /locus_tag="PLU55_01715"
     CDS             complement(13456..13800)
                     /locus_tag="PLU55_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86810.1"
                     /translation="MVTIIQSVFNKIKSIVFRESTKATKTKKSISNNKELVESKKWYH
                     LSFFCRGTDFQPVYFYIFCLFWVLMFFCFLKARFIFIEDLTKDVSETFILSLLGTFVT
                     TFGMFNIFNKKD"
     gene            complement(13809..14042)
                     /locus_tag="PLU55_01720"
     CDS             complement(13809..14042)
                     /locus_tag="PLU55_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86811.1"
                     /translation="MNLFEDDKTKIKVKIKPKFNTKKLIKEHEVSLEELQLGAEMDYI
                     FDDTTSMNMKGKIIINNKDIKVNQASINFTKKF"
     gene            complement(14146..14880)
                     /locus_tag="PLU55_01725"
     CDS             complement(14146..14880)
                     /locus_tag="PLU55_01725"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HPJ86812.1"
                     /translation="MITFKEGLKNLKFNKEEILLFLQVCINSYDGKHGKVMDVLQDEK
                     RISVNGVTIILGKYLDYDVICFRGTKGDDEWALDFNAEFAKPPFITINNKIKTHLGFT
                     VRYKTLNEWLKRYIKENNLKSILFLGHSLGATTAMLASDEIKQTYTYIKTTVITFGSP
                     RVGNQEYVDFHFKSVPNTLNFRNKNDIVCRVPTEKMGYKHCPYYVWIAKPSFWQKLLH
                     PINYFKGCKEDHRPTNYYANIKKEKV"
     gene            complement(14890..15615)
                     /locus_tag="PLU55_01730"
     CDS             complement(14890..15615)
                     /locus_tag="PLU55_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86813.1"
                     /translation="MEAIITTYITATILNKFIVLLTAVLFFGFVYWFTFKVLSFFKNT
                     KKVKIGNVEFSNLEEKKINYPKNHLECPNIASLFLLEELRDRYHEDKMEIADRYTINL
                     EQMIVARTTIDSVFVNWGEEIKEDDIPYKRLILENIRNMVLNKVTEVLQENHLAEMSE
                     EDFLKRTKQRADAISSIIFTSSLNNKIEEDMKGMLKYCIMDIIKKARDIQVNRNNKVE
                     KIKREYKEKREKILKGEILEVCA"
     gene            complement(15627..16238)
                     /locus_tag="PLU55_01735"
     CDS             complement(15627..16238)
                     /locus_tag="PLU55_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86814.1"
                     /translation="MEFNTIKDKLYKIYTEKEIKKQTVNIFGVRKSDSNSLRNMYEDV
                     IGFDINGEILIFEGTTNPGKYYTVNPINKLGCAHVKEGYHPDIWQVGIHLPDTRNAHE
                     AFIQTGNKITVLRDKNENGIIDNDEVEYVGYFGINLHGGALSEDDNINGWSAGCQVLR
                     RRSELLNIVLPKIKSLPEFKSNPKMKFSYLLLDEKILPEVFVF"
     gene            complement(16240..16365)
                     /locus_tag="PLU55_01740"
     CDS             complement(16240..16365)
                     /locus_tag="PLU55_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86815.1"
                     /translation="MGLRTIFFKEKPSKNYVQTGEKTTASVDNIIKVYNLEIGVS"
     gene            complement(16356..16736)
                     /locus_tag="PLU55_01745"
     CDS             complement(16356..16736)
                     /locus_tag="PLU55_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86816.1"
                     /translation="MESCILLQEDKNSKSLKDKIESLEIELIKLLDQLDDLKDDILDI
                     NEQIDKEAKRRKELRKKDDDKNKEEIQNSLDKEDALEEKRRSILEKSVPILERAKKVF
                     QKYKDAKEKNKPKKSEKDKEDSWD"
BASE COUNT         5441 a         2743 c         2141 g         6397 t
ORIGIN      
        1 cagtgcctgt accaacagca actttgtgat cagtatgtct aataaataca tatattgaat
       61 atgcggtact gccataattt gtatgtggta aagtcaaatc agtattgcca tcgaacgcaa
      121 taccggaaat gcttacaatt gtatacggcg tttgtatacc atgcgcgact tccttttgtc
      181 cagcattcgg caacgccccg aaattaacaa cttttctgta tttttgatac cccaaaaatg
      241 ttccttttat aatcgtttca gtttcccttg aaaaatcagt taaaacttca taagtccatc
      301 ctgtagaaga gtatgtaaat tttactttac attcaaaatg aatatctatt gtttcaactc
      361 caccaaaaga aacagttaat ttgtatgttg atgaagcatc attacttatc aaaacttcac
      421 aacctacaaa ccttgcacca gcagttaaat taatggttct atcagcagta gggacaaaaa
      481 ataagtcagt attattttca aattttgaag ccgtgtaatt tgcatcaagt tcacgaatta
      541 tttctttttt catttcactt gagtcaaaaa taatccaacg tgtcccattc cagcgtaacg
      601 caacataacc atttggcaaa acgtcaaaag tgtttgacat ataataagtc aaggtaactt
      661 tataagtatc actattgtta taaatatata aatcaatatc tgcaaaatgt gctcctgctg
      721 taatatcaag agttctatct gctgtaggag taaaaaataa agaagtatta caaataagct
      781 catttgttgt ggtattaaca tcaattccag ttgtaatatc cttatttatt tttttatttt
      841 taataaaagt atcacaagca atattgaaag tattaaggtc tttattgtat tggggggctg
      901 tgtgcgcttc actataatca ataccattaa caacccctcc accatcaaac aagactttgc
      961 atataattat atctgttgac tgaatatcct caaaagctac ttggtaataa tcaggataat
     1021 ttgaaataac atttgaatat gtatatcgca taataacata aggtgttgct gaacttgcat
     1081 ttcccccaaa agttgtaaca gttgaaaggt caattgaaga agttgttgtt atagtagtag
     1141 ctttatcatt acttgactca aatattgctg tatatggtgc aagagtaagt atatttccga
     1201 tataagagac tgtacctcct ttataaattc ctgaaggaca aaccccccta agaacagaat
     1261 taaaattttc tgctttagca ggatgtttat atctaaaagt tatatttgca tttccataat
     1321 tgtttgcgcc cattggtttt atcctctttt cttatattag tataattttt ttaatcaaac
     1381 ataacaaatt tcaaattgaa tattgttgta catattttcg tcccattgta tttttggaaa
     1441 agtgctataa agtaccaaac cactattttt gttatacatg gcaatttcag taaattcagt
     1501 aaatctattt ctttcatcta tattaaaatc aaaagcaatg catcctgaat tgttagtttc
     1561 actaaaataa ttaataattc cacttaaagg tataactcct gaaatgtaat ctggataacg
     1621 ataagaaaaa ttattaaccc ctgttatagt accactaatg ctttgtgtac ctgttccaaa
     1681 ttctagatag tataaatcct caatatcatc agaaattaaa atattgtatt gattagctgt
     1741 taaattaaca ttataatttg agtatgtttg tgttacagga gttaaatctt ttttaacatt
     1801 tatttctaat ttaggtgtat aataaattac ttcagtaact ttgtgtattt ggtctatatc
     1861 atttttcaaa acttttaaag tatttaaact cataaattca ttttcatttt caacaaactt
     1921 atgtttataa ttaaaattta aatttcttgt tatttgagtt atgattgact cccctcctcc
     1981 tgtttcttca ccagtagcag ggtcaagaga tataaaatca tcagagtcta aagtagttgc
     2041 ttcttttcct gtttctttgg ggtcatcata aaattcaaat tgagcatcaa gtgtgtataa
     2101 atcattgtca gaatctaagg taaaatcaca atcttctgaa tcacaatcaa gttcccaagg
     2161 gtcaaaatta acatagtaaa gaatattatc cccttcttgg tcaagttctg taaccgcata
     2221 agttatttgt gtgtcaggat tataaattgt agataatttt gaagttgtta atcctcctgt
     2281 gttggttgcg gatatagcat aaaggtttat aagtaaatca aaaacataac ctgtataagt
     2341 gtaagaaggg atagcatttt tatatttaat tctaggtact attgtttgaa tttctttatt
     2401 taaaaaataa tctgttgaag tataaccatc aaaagttaat aaatgaaatc caaaagtttt
     2461 agctatctct attttttcat ctcttgttgc cttattcatg tcaaattgat taaatatagg
     2521 aagcaccttt tcctgtttaa gttgtaccca taaatcttgg acaacatcaa gaagttcccc
     2581 ccatcgggtg tttctcattt ttagcggaag taattttcta aaacttaaat tcattcaaaa
     2641 acctatcagt ttgaaagagt tgtaaatata aaatctttat ctatatctgt aatttggtat
     2701 ctatatggca atcgaacatc ctgtgtttgt aaaccatatc catttttagt attataagcc
     2761 ataactatca aataaccatc attttgggat tcacttggat tctgaatagg gtattgtgtt
     2821 ggattatcta ctattgtttg tattacataa ttataatatc ctgtagaata atttatattt
     2881 gatgaactta ttgtataacc attcattcca ataaaacttg ttccaagtga atatgcaatt
     2941 tgaacaggtt cttgccaaac ccctcctatt tttcttttaa tccataattc aagactgtta
     3001 tttactaaag atatttcttg tgatggtaaa gatgaaacaa aaaatgcatt tacaagtcta
     3061 tctgtttgga tagcattaac attatagtcc ataagtttaa tagttgtttc atggtagtca
     3121 atataagtta agtcatctat atcattaata taattagaac tataaatatt agtttgataa
     3181 tttgtattta atgtagaata ggcatcataa atcaaatctt taatttggct tgacactata
     3241 gaagtggtgc tgttagatat ttttgcattc acattaaaca ttatatatat tttttgagtg
     3301 tttgtaaatt gtaatatctc agtaggtgat ttatatgtat aatatatttg attttctaaa
     3361 taagtttttt gtgccggtgt taaattatca ccagtgtttg taagtgctgt tgcaaatact
     3421 ttattttgtt cactcattaa agtagaacca cctatatcat taacactcca aacaactgct
     3481 ttataaataa aactgaaatt ttcaagaatg gttttccaat cattagcagt gccacatcta
     3541 tatcctgcaa aaaacaaatt aggtgcattg tttcttatac tctctatatt ttcataatca
     3601 gcaccatcgg aaatagcatc aacattatta caatataatg taattatatt atcatccaca
     3661 tcttttaatg tatctttaat tactgtaatt gtgttagcac tttctatgtt accattagca
     3721 ccatctgttt ctacatattt aatcattact ttatcatttg cttcaagttt tttgcttgat
     3781 ataccatcac caaaagtaaa agtaacccct tgaaggtcat cgaaagaaac agaggaacaa
     3841 ttataattaa cagtgtcatt aacaaagtac attttatcta ctatgttcac ttcatatagt
     3901 atttcattat cattatttac tatataaaca tcaaaaatat cattgtctat atcaggagaa
     3961 tatattttta tttcttcatt agctacccca cttgcagtat agaaaaaagt ttttggtgtt
     4021 ccttgtcgaa cttgtacttc taaatttcct tcagtaccat taaaataata atagtcctga
     4081 attgcataaa cttttttatt tcctgaagag tcggtaaact gtgtatattt agggatataa
     4141 acattttttc ctgtataaat ataagcagga ctcatatctt catctttaga aacaagaaca
     4201 acaccattag ctcctacttt tctatgtact gtataagaaa aagatttaga cattatattg
     4261 agggatttta aatcgttagt tttaataaaa gaggcattag aatataaaat atctgctgta
     4321 taagctaatt tttccataac ataagcaatg gcatcgagta ttctttcata aacaccaaag
     4381 tatatgattg tattccattg agacattaaa cttaatttag ttttaacagc ttctttaatt
     4441 gaatcaaacg tccaaatcat tttttaaacc ctcattttaa gaaacattac acacataaag
     4501 tattctttca aaatattcat cagtatctac taaatttata aatttatatt taccccaaac
     4561 ccatgaaaga acatcattat cataaattaa tttttgagaa gggtgtgaat acttttgaat
     4621 ttttacaaat tcaaataaat tttcttctat ataatcaata tcaaggtaag tattactttt
     4681 tttatctatt actttgtcca ttgataattg ttctgtaact actgtattag taccgttgat
     4741 tttatacact acatctatat ctatacttct atttgtatag tcaggttgta ttgaaatatt
     4801 tacaagtttg actaaagaaa caagatttat tgtaaataga ttttgaatag caaaaccaat
     4861 tagttctaaa ttagagtttg tcatattttt aaatagaaaa taatctaaaa ttccaccttc
     4921 tgatggattt tttaaatagt cacctctttt agaaactagc cattgagaaa ttgcattttt
     4981 aatagcttca tcattctcat aaacaatggc gtttccatct ataaaagtgt ttccttcaat
     5041 gtcataatca aaataattta ttgccataag atattagtac tgttaattat tataattttt
     5101 ttctgaaagt aaagtatcta attgtgctct taatatttca aaggatgggg cattagaaac
     5161 accccctgtt aatccaccag taactgtaac agttattcct tgaatagcag tcagtagact
     5221 atcaaaaaac tgttttactg tttcaccttt taacattttt tcataagcaa cactacctga
     5281 ttttaaataa caaccatcat tatctatttt aaaatagctt cctgaaggat gatatacaaa
     5341 tatttccggt gaatcatctg aactatcaac accaatacaa attccatttt tataataagt
     5401 gtattttgtg tttgggtaag aagaactact ttctatttca ctttttatac tatcttcaaa
     5461 taaattatga ggatgaattt ccgacagttg tatatccgct atataaaaag gtttttgcat
     5521 gtcgggttca tcataaaaac caatccacac tttagaatct ttttcaggaa tgaaagattt
     5581 tccatattca tttgagccac caccaccaat acaaaattgt gtagcccaag gtagtaaatc
     5641 atttgttgta tttttatgaa gataaggaat tttaatttta attctgcctt ttttttcaga
     5701 atcattatta tctataacat cagcataatg taatttaaac cccaaaccat tcattaaact
     5761 tctccttact ttttatttgc taaaataaaa tcatcaattt cctgtttatc gggaattata
     5821 atttctgttc cggcaataac atcaaatata tcttctatgt tattcagtat tagtaatata
     5881 tcttcataat ctatagtgcc atagtaaaaa gcacttacta aataaggtct taatatttca
     5941 tctttagtta aggtgtgtct tttaaattta gaactgaatt taaaagtact ccaatttaaa
     6001 gaacggctat cataaatagt gacattatta tcatcttgaa gaacattata aactaattct
     6061 ctcattttgt ttcccctatt tcttctttat gtgaagcctg ttgagcttct ttagcttttg
     6121 tagcatcatc actttcaaaa tttattgtag ggtaagcatt ttcatttgaa gaaacaagac
     6181 cattcttttc ccaatcttta gaaataatat caattttatt tgaatcatca agcatatcat
     6241 ctatagctga gtaaattcct gaaagtctta attgtatttc cccccataaa gggtattctt
     6301 ttccattatc agcaacaaca acttgatttg agtatgttgg ggtgacttct tcaacaaaac
     6361 agtaaggaag ataaataacc ccagggatta atactgaaag tattttagac tttatttctg
     6421 aaaaatctaa accaaatcct gcttgtttcg cattacttga taaaaattca cttagtggtt
     6481 ttttttcttt aggtgttgct gtggtggaaa tatcaccatt taaggctctt gatatttgac
     6541 cacaagaacc taaacttggt gcaggagtaa cgaaagaacc tcgtttttta tctcctgttc
     6601 cttcctctat tcgagaaaga ataccaagac ttaaaaagaa attcatagga agccaaacat
     6661 cagtaactac atcttctctt gtataaaatt ttaaagtaca gtttattgaa gcaggatatg
     6721 ttttcatcca acgttttaaa tccataacat ttgacatata cacagtacca gcacctacag
     6781 tacttccagt agaggaataa gaaattgcaa gatttactaa atttgttata ccccctggaa
     6841 gcaattcacc gaaagaagaa aattctgatt tagcatcaat agtaaatcct tcttgtaaaa
     6901 gaaaggattc tggttgtatg ttacttttta atacttctac aaataatctg ttcattgttt
     6961 ttccttttta agagaagctt ctgtttgtgt tccacctgta ctgacagtaa gatttgtttc
     7021 ttttttcata tttccttctc ttactgcttt aacaagctct tttaaatgtg caatttgttg
     7081 accttctccc ataattttaa gattttttat actcttataa aaatccattt ctgcaccagc
     7141 ttttatagat tcttcactaa cttttccttt tgcaatatta tgtagtctat catcatcaac
     7201 tttatatttt tcagaataag cacgaatata ggcttgttca gttatgtatg cttcagcaga
     7261 tgatacttta taagcatccc ctctatcttc catatttgga atgtattgag atatatgata
     7321 tattagcata tcaaataatc ctaaaaatgt atcaaataca gaagaaaagg catttttaaa
     7381 agcatcttta actgaagtaa aagcacctgt tatcatatct ttagtgaaac ctccagtttt
     7441 taatttttga aatcctttta ctacttctct aattaaaaag acaaaaaatc taaaaggtgc
     7501 tattaatgct ttaattataa ctgcaagtat tttaaaacct gttataacca ttttggaaaa
     7561 gacttcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncaat acctactgga
     7681 ttaagccctg caagaagttt tcctatatgt ggaagaaatt tcattaaacc acttataacc
     7741 ggttgtaaga atctaaccgc tgtcataatc ataggcagaa attttcctat aagtaatcca
     7801 aaagcaagta accacggtaa aattttagaa agaggtttta ttatttttct taaaggtgaa
     7861 tcctttgcta ctcccatagc atcaagtatg gtgttaaata tagtattaaa gaaaggtggt
     7921 aaaacatcct taagtatatg aattaaaaat ttaataaatc ttggtaagta ttttattatg
     7981 gctgttatta atacttgtgc catattaatt aaaataggaa ttaaaacatc aagtaatcct
     8041 gtttgaatta gaacaaatat accaagaagt aaaccaagaa aatctcctat accaccaaga
     8101 gacattcttc tcatgaatcc aaaaatacct tcaaaccatt tattcttaaa tacttttgtc
     8161 catccttcag aaagtttgtc tcttattttg gctttttttc cagtatttgt ttgctcatac
     8221 atactaccaa accattccat attttcagac attctttcag taacatctaa ttgttcttta
     8281 tcggctttac gttgtgcacg tttatccgat ttgaaggtgt cattaacatc ctctacaaac
     8341 cctttaatgt ctgaaagtgt ttcatccggt gaaggtgttg gttttggtaa tactactgtt
     8401 gccatttaac ctctatttct tacccttatg agctttttcc atttcttcat ttactttttt
     8461 ttgatactca accaattgac tatatctttt ctttatcaca taataaaaca tgttcataat
     8521 ttcactttct tgctgtttca attcacgctc caataaaaat attaagcttt gtagactttc
     8581 ttgattctct tgtggtagca atgtttgtag gaagaaagta ttgtatatca atttccctcc
     8641 gaagtaccct cttgcttttc ttgccacata aagggcagga aagctctctt tcatcgtcta
     8701 agccgaattt aatctggtct aatgaatatg taaggtcaac aaaaacagaa gaactcattt
     8761 cttctaattc ttttactttc tgttcatttg aaagtggttc accatttttt gttaatagag
     8821 aaagtgcttt aacataggca ataaagtctt tacttttttc tctctctact ttttcaagtt
     8881 cattttcttt ctttctctgt aattcagcaa gaggaacact atgctctttt cttgtttgaa
     8941 tatttttaac cacattataa tatttttttt caaccaattc atttgctata atcatgtgtt
     9001 taattcttgg aaattcaaat tcataaacat tctttccatc tgccacaatg aaaggttctt
     9061 taatttctat tttttctaac tcatcctcta ttttatattt tgaaatatca atatcacttg
     9121 ttttatattt cattttaaga taagttgaaa agttttcagg attttcaaac atatctttat
     9181 aaaattctct aagtttttta tctgcatcag aaatagaagc aaactttagt gtacttaatt
     9241 ctatattatc tgtattagct actctatctt tttcaggaac atcattttga caatcacaca
     9301 accaataaaa aggaaagtaa ttcccctgat aattagcttt aatgtttaca agtatttcaa
     9361 gaagctcttc aggtgttgct tcctgtaaat ctacatcctt atatttttca tgtttatttt
     9421 tatttaaaag tgttacaagt gtttcaagtc tttcgttctc tttacacaaa gtcatatcac
     9481 taacatcttt acctttgaaa tcataaaaat tcatctgctt tggaagagaa aatcttccct
     9541 ctgtttcaaa gtcaacaacc gctgttcctg ctgtattaaa aatttctttt ttatcaacaa
     9601 ccccttctac ttcttgtggt ttgttttctc ttcttttaat aaattcactc attcttttaa
     9661 tctcctatat aaaaaatagt atttcaagta ttagtaggga ttaagtaaat atacctgtta
     9721 ctgcattttt aacatcttca aaacgtaaga accaacacat atcaacacta aatataacat
     9781 ttaacttcat tggctcacct tcatcctgtg caaaagtagg tgggtctata tttttaaatt
     9841 tcattccaca aactttaatt gcaaaagtag gtaagcccat tcttgtttgt ggtataatcc
     9901 aacaatcttt tttactcgct tcctgattat ttttccaacg tactctacca tctgttgtta
     9961 cttcaaatat ttcatcttgc cacgcatcaa tccatgttct aactgtcccc atttctgttt
    10021 caataaatgt aatagatatt tcttcagggt gaactaattc tttaacataa tttttatgtg
    10081 agtttctttc atactctata cctgaaaact ggggtgttat agagttcgct aaaaatgttg
    10141 caactgcttg gtcaagaatt gctgaagtgt taactctttc taaaacattt cctactgaag
    10201 aaaataattg agaagcaacc cctgttgaac ttgttgaaga agcttgtggt tttttacttg
    10261 catcttttgt gtatttttgt ataaatataa tttcaaattg atttagactt tgaaaattaa
    10321 tcaaagagaa tatttctttt attcccatgt tgattaatcc aaaaggtttt cttgtgtagt
    10381 ctaaagctgt aactatactt tcaggtaaca aattaaaact cctcttgtaa ttaaagcaca
    10441 cccaaaatta attgagtgtg cttgtgtttc ttattccatg tccatatcat tgaaaatgaa
    10501 tattgcagta actctcactg ggtctgcccc ctgattatca aaagtaccta ctttcaatga
    10561 ttttatttta gcatcaataa attttaaagt tttaacagta ttaagttgtt tatcttcagc
    10621 tataacatat atatctgttc ttgttgctat ttctggaaga gctgttcctg tttcataatc
    10681 ataacaaagt ttatgccatg aattaagtgc atcgtaaaca gcccattctt ggtcaagcct
    10741 aaattcaatg gtaagactgg atttatccat atcctgaacc atattagtgt aaaccatctt
    10801 tacacctttt ttgaaaacct cataactacc aactgttctt tcaggtgggt caaattgtgt
    10861 gtctgctctc aaagaaagca aatctgtatt accaccacca ggtattcctt tcggaaaaat
    10921 aatttcaaat tgtgtttgta attggtcact tccaagtttc atcaaatcgc ttgctattcc
    10981 catattgtat aatcctcttt aattaatcta tatttttaaa gagaatggaa ggtttaattt
    11041 ccattctcct tttcttttta agaaactgca cttatttcaa tgttaatagt tccttgtggc
    11101 attcttgtaa atctaagttt aacttcctgt gaatttgcca ttgcaacaac atatacatca
    11161 agaaggaatt gtctttggtc aagaacatcc gctgtattgt ttgaagcatc gcaaacaact
    11221 ttgaaatcag ttatacctcc ttcccttctt acaggattaa gaatagcttc tgtttttact
    11281 ttagcagttc ttctgtgtgg aatatcgttg tttttaaatt cctgaagtct aagaacttgt
    11341 tctgctatgt tttttataat gtatttatat acacgtcttg ttcctacata agaagtatca
    11401 ctaagtgtaa cttgtgatgt tttatctcca taaatcataa gaccataatt gatgtccttg
    11461 attataggat taatttgagc atcatcaaga agttcaagtt ctgaattaaa atcagaatca
    11521 gtgtaatcat attcaacttc aataacttcc caatctgcaa gctgtccacc atgtccattt
    11581 tcatcaattc ctgctggtga acctgaatca aaaacatctg ccattttagc aaatttttta
    11641 cctactgaac ctatgtttga aatccatgca aaactattgt tatagttatc tttaattttt
    11701 gcccagttag tatagaatcc aatatcatcg gaatcaattc caagtccctg tctaaaagct
    11761 actgcttctg ttgcattatt tcccatagga attgcacaaa ttccctgtgc gtaaggttga
    11821 taagtctgaa taagattatt aactgttact gctgaatcac ctcttgtgtc catcataatc
    11881 cttacaggat atttgttagc aaatctaaaa ttgttccatc ctgctaaaat atcagcagaa
    11941 tcaatttcgc tacctcttgt accaccagaa aagttagcag taagtccaga aagattatat
    12001 gctgttcctg taaagtttga atttactttt ggaataacat aatagttatt atcaaaaaca
    12061 tccatgatat aaagtgattt accaaaagca tctttttcaa tgttcaatga atacacataa
    12121 gaattaagta attcatttcc aacacttgaa gatttatata aatcaagttt gaatttttta
    12181 cctgaaatat aagttatttt tcctacaaga ttatctgtgt aaggtgatgt tgcaaagaaa
    12241 gcgtgtgata catcagggaa agcatcaaaa gaaaaagtgt caatatttct tcctgtacca
    12301 aaaccattta cacccgaaat ttgaacatcc ataccaccga aaagtgcgtc tgaatgataa
    12361 ggggctgata tataacaagg agctacacta caaaaagcaa ttgcctcaaa tatatcaggg
    12421 taacttgaag aaggtgcacc atattctcta agtatatctt cttctgaagt acaaagtacg
    12481 ggtgtttttt tcccttttgg tgctttaata accattgcac caatttcaaa gatattaggt
    12541 tgtacaaaac ctgaatagtc tctttcttgt gttttacttc tatacggcat tatttttaaa
    12601 cctcttttaa tttttaaatt taaagttttt tctcttaata ttagtattca aaaaattgaa
    12661 aattctaata ctaataaatc aaagaggatt ttaaattatg ccaattttta ttgaagatac
    12721 atatcttaat caaattaaag cttgtttggc ttttccagat gttgacaatt tactattaag
    12781 tgatgaccaa attaaagata tttgtattgc cccttctcta agagaatatt tttctaaatt
    12841 tccaataaag catgagtatc aagaacctgt tggttttaat gaagaaaaaa cagtttcttt
    12901 tccagacagt tatgtttttg gagttttaac ttgttgtgtt tcagatatag gaaacaatgt
    12961 agtggggagt ggtggggatt tttggactat tgttgcttat aactcttttg ggatgggcaa
    13021 tattaatgtt atgaggggaa gcggtggaat gtatggcata agaggttata atccaaataa
    13081 tgctatggaa atgaatgaaa tgtatagaga taaatggaag agctaccaaa acaaatatgt
    13141 tacattaaaa tttcatcctg attatgttaa aaaagaaatt aaagtttatt ctactgctaa
    13201 tggtatgcta aatattacat gggcgaaaca tagtgaaaat tttcaagatg ttcaatttca
    13261 atttataaat gatgttattg atttgtcaaa agcaagactt cttgaccatt tagctgatac
    13321 ttcgggaata ctatctgata caaatttaga aataacaatt aatgctgaag acttaaaaga
    13381 taaagcatca gcattgagag ataaagtcat agaaaaatgg agtgcaatac cagatatagt
    13441 atatcttcat gtagtttaat cttttttgtt gaagatatta aacataccaa aggttgtaac
    13501 aaaagtaccg agtagggaaa gaataaaagt ttctgaaacg tcttttgtta aatcttcaat
    13561 aaagataaat cttgctttta aaaaacaaaa aaacataaga acccaaaata gacaaaaaat
    13621 ataaaaatat accggctgaa aatcagtacc acgacagaag aaagacaagt gataccactt
    13681 tttactttcg actaattctt tgttgtttga tatactcttt tttgtttttg ttgcttttgt
    13741 actttcacga aatactatac ttttgatttt attaaatact gattgaatta ttgtaaccat
    13801 gtgtcctcct aaaatttctt tgtgaaattt attgatgctt gattaacttt aatgtcttta
    13861 ttatttatta ttatttttcc tttcatattc atgcttgttg tatcatcaaa aatgtaatcc
    13921 atttctgccc caagctgaag ttcttctaaa ctgacttcat gctctttaat caattttttt
    13981 gtattaaact taggtttaat cttaacttta atttttgttt tatcatcttc aaataaattc
    14041 atttaaaaaa actcgtgtcc cccaagctgt ctaatttaat tatagtgatt gtcattacta
    14101 ttaaacctaa aacacatcca cctaattgta gaattatttc taacattata ctttttcctt
    14161 ttttatgtta gcataataat ttgttggtct atgatcttct ttgcaacctt tgaaataatt
    14221 tatagggtgt aaaagctttt gccaaaaaga aggttttgca atccatacat aatatggaca
    14281 gtgtttatat cccatttttt cagtaggtac tctacaaact atatcattct tatttctaaa
    14341 atttaaagtg tttggaacac ttttgaaatg aaaatcaaca tactcttgat ttccaactct
    14401 tggacttcca aaagtaataa cagttgtctt aatataagta tatgtctgtt ttatttcatc
    14461 acttgctaac attgcagtgg tagctccaag agaatgacca agaaataaaa tactttttaa
    14521 attgttttct ttaatgtaac gttttaacca ctcattaaga gttttatatc ttacggtaaa
    14581 gcctaaatgt gttttaattt tattattaat tgttataaaa ggtggttttg caaattcagc
    14641 attaaagtct aaagcccatt catcatctcc ttttgttcca cgaaaacaaa ttacatcata
    14701 gtcaagatat tttcctaata ttattgtaac accattaaca cttattcttt tttcatcctg
    14761 aagaacatcc ataacttttc catgttttcc atcataactg tttatacaca cttgaagaaa
    14821 aagtaaaatt tcttctttat taaattttaa atttttaagt ccttctttga atgtaatcat
    14881 aagacctctt taggcacaaa cttcaagaat ttcgcctttt aatattttct ctcttttttc
    14941 tttatactct ctttttattt tttctacttt attgtttctg tttacttgta tatctcttgc
    15001 ttttttaatt atatccataa tacaatattt aagcattcct ttcatatctt cttcaatttt
    15061 attattgaga ctgcttgtaa agattataga ggatatagca tcagctcttt gcttagttct
    15121 ttttagaaaa tcttcttctg acatttcagc caaatgattt tcttgtaaaa cttctgtaac
    15181 tttattaagc accatatttc ggatattttc taaaattaat cttttataag gtatatcatc
    15241 ttcttttatt tcttcccccc aattcacaaa aacactatca atagttgttc ttgctacaat
    15301 catttgttca agattgattg tgtatctatc cgcaatctcc attttatctt catggtatct
    15361 atccctaagt tcttcaagca aaaacaaaga agcaatgttg gggcattcta agtgattttt
    15421 aggataattt attttttttt cttctaaatt agaaaattca acattcccta ttttaacttt
    15481 ttttgtgttt ttaaaaaaag ataagacttt aaatgtaaac caatatacga aaccaaaaaa
    15541 tagtaccgct gttaatagta ctataaattt gtttaaaatt gttgctgtaa tatatgttgt
    15601 tataatagct tccatgctta ccttttttaa aaaacaaaga cttcaggcaa aattttttca
    15661 tccaataaaa gataagaaaa tttcatttta ggattacttt taaattcagg tagacttttg
    15721 attttaggca aaacaatatt aagaagttca gaacgtctgc ggagtacttg acatcctgcc
    15781 gaccaaccat tgatattatc atcctccgaa agcgcaccac catgaaggtt aattccaaaa
    15841 taacctacat attctacttc atcattatct ataattccgt tttcattttt atctctaagt
    15901 actgtaattt tatttcctgt ttgaataaat gcttcatgtg cgtttctagt atctggaagg
    15961 tgaataccta cttgccaaat atcagggtga tacccttctt taacatgagc acaacctaat
    16021 ttattaatag gattaactgt gtaatatttc cctggattgg ttgttccttc aaatataagt
    16081 atttcaccat ttatatcaaa accgataaca tcttcataca tgtttctaag tgagttacta
    16141 tctgattttc ttacaccaaa aatatttact gtttgttttt taatttcttt ttcagtatat
    16201 attttataaa gcttgtcttt aattgtatta aattccattt taactaactc ctatttctaa
    16261 attataaact ttaattatat tatcaactga agctgtagtc ttttcccctg tttgaacata
    16321 attctttgaa ggtttttctt taaagaaaat agttctcaat cccatgaatc ctctttgtct
    16381 ttttcggact tctttggttt gtttttttct ttagcatctt tatatttttg aaaaactttt
    16441 ttagctcttt caagaatggg tactgatttt tctaagatac ttcttctttt ttcttcaagg
    16501 gcatcttctt tatctaaaga attttgtatt tcttctttat ttttatcatc atcctttttc
    16561 cgaagttctt ttcttctttt agcttctttg tctatttgtt cattaatgtc aagtatatca
    16621 tcctttaaat catctaactg gtctaaaagt ttaataagtt ctatttcaag actttctatt
    16681 ttgtctttaa gactcttgct atttttgtcc tcttgtaaaa gaatacaact ttccatgtgc
    16741 ctctgttaat aacgataatt tataaattca ataattaaat ctgaagaggc tgtagcttct
    16801 cttgctttaa taagaaattt at
//
LOCUS       DAOPCH010000014        15941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_3736_length_15941_cov_4.549540, whole genome shotgun sequence.
ACCESSION   DAOPCH010000014 DAOPCH010000000
VERSION     DAOPCH010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 15941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15941
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3736_length_15941_cov_4.549540"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1472
                     /gene="lysS"
                     /locus_tag="PLU55_01750"
     CDS             15..1472
                     /gene="lysS"
                     /locus_tag="PLU55_01750"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPJ86817.1"
                     /translation="MGREEQIISERIRKLEELRKEGINPYPNKYDKKQTCLECSKSKI
                     KSKVKTAGRLMTKRDLGKIAFCVLRDGTGDIQLVIQEKETPEKFREFFKKYLDSGDFV
                     GVEGEIIKTKTGEISILVKKIEIISKSILPLPEKWKGLQDEEERYRKRYLDILMNKDV
                     KEMFVKKAKFWNSIRNFLLEKGFLEVETPVLESSAGGAAAKPFITHHNALDLDVYLRI
                     SMGELWQKKLMVAGYEKTFEIGRQFRNEGMDMEHLQDYTQMEFYWGYANYQDGMKLVE
                     EMYKKVAKEVFGTMKFDIHGYKVDFSKKWEIYDFETLIKKETKIDIYKADVNEIKKKL
                     NELKIEYNPSVDKWRLVDQLWKYCRKKISGPGFLTGQPVELSPLAKRNPEDKRKVEQF
                     QVIIAGSEVGNGYSELNDPLDQEERFKEQSKQKEAGDEESMEHDKGFVEALKYGMPPT
                     CGFGLSERLFAFLMGKTLRECVIFPLMKPEINKEK"
     gene            1475..1660
                     /locus_tag="PLU55_01755"
     CDS             1475..1660
                     /locus_tag="PLU55_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFYB/HAP3 family transcription factor subunit"
                     /protein_id="HPJ86818.1"
                     /translation="MGLIIKSNIRPRVKELDKENVISSVADEVEIALERKVEEILDEA
                     IKRAKANGRRTLQARDL"
     gene            1672..1875
                     /locus_tag="PLU55_01760"
     CDS             1672..1875
                     /locus_tag="PLU55_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86819.1"
                     /translation="MINPVFFYILGIIIFVLLLIFGLKFSKYKIKYIKYGGYLLIIIG
                     LLGLVIDLYNIFNKYLMPSIINK"
     gene            1877..2065
                     /locus_tag="PLU55_01765"
     CDS             1877..2065
                     /locus_tag="PLU55_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86820.1"
                     /translation="MAVRRYRRTTYRRRFPVLGFIILIFATLWLLREMDIIDLYVPWL
                     PVVLIIIAIGIIFNRLIG"
     gene            complement(2106..2921)
                     /locus_tag="PLU55_01770"
     CDS             complement(2106..2921)
                     /locus_tag="PLU55_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86821.1"
                     /translation="MRELTDILGQPIESRTDFNKWNSKSSCDTYLPNSPIYLKKINNA
                     DSEQSQKIDYFIERELKWLDSNEYISRKKEATGMSESKIKKDIQKIKEQLAKTKLNIS
                     NKKDDLAKGVYYQDKKNPVINIFKDGSRYRTLNAIDHEIKHAVSEQALETMDDLIKVF
                     TSNYRRYPKINNKRVIDYLIPVETLGKWASKAPEQQVISKRIMDIMEIDYGIKRGSKL
                     TTDNLKKLISDLNHQIKTRDERNSDIIAMLYKMKQKHKDSYQSKLCEMVNNAY"
     gene            3109..3588
                     /locus_tag="PLU55_01775"
     CDS             3109..3588
                     /locus_tag="PLU55_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86822.1"
                     /translation="MVAKKGVDIKKQTKSAHSSQGKKNRAAGRRFEAKVRENLEEMGW
                     IVNKWMNNIDYEKNKIVPAKRKYNPFLKALSIGTGFPDFVCFKKDETGRYEVIGLEVK
                     SNGYLDQSEKGMCLWLIENKIFSRILIAKKNMKGRKIEIEYIDFSEKYNSKQQNTEQ"
     gene            3620..>3985
                     /locus_tag="PLU55_01780"
     CDS             3620..>3985
                     /locus_tag="PLU55_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPJ86823.1"
                     /translation="MRKKFLFFIVFFIFSIINFSLISASCQKNQININTASLEELDKL
                     AGIGPVKAQAIINSRPFNKLDDLLNVVGIGDVTLSNIKQQGLACVSGEEEEEEEQEDN
                     EEEDEEKEDKKETIKQIKKI"
     assembly_gap    3986..4085
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4086..4356
                     /locus_tag="PLU55_01785"
     CDS             <4086..4356
                     /locus_tag="PLU55_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86824.1"
                     /translation="EDEEKEDKKETIKQIKKISVNPEENKIQEEQEIKPIILSPISPK
                     SIKTEPYKKELDKNKFAIYGLVFLGILVFFLFKMKKQKNEKTEFK"
     gene            4334..4810
                     /locus_tag="PLU55_01790"
     CDS             4334..4810
                     /locus_tag="PLU55_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86825.1"
                     /translation="MKKQSSNKEDSGKKVISSTIILEIIGRPAEQLVTTLEGLIKKMG
                     EEKGVKVTNKKINEPVLIKDTKDFYTTFAEVDLEVEDILYLAIIMFKYMPAHVEVVEP
                     ELIALTNNGWTDILSELTRRLHGYEEVARVLQFQNSEMQKKLKELLPENKDQEKKK"
     gene            complement(4814..7837)
                     /locus_tag="PLU55_01795"
     CDS             complement(4814..7837)
                     /locus_tag="PLU55_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86826.1"
                     /translation="MKREVTLLLVFTLFLIPLSTINAADELKGLESAYTCLDKRINST
                     GCSALKFEEKVFSYLASGNCGNELSLENRSNECWPKSGCTLKSTAQAILALNSRADTK
                     KAENWLLKQTTSPENIEWFLEIESKSGATLCDISYSGATYKVTIGEDRKISSNAGNHL
                     TLAQDGYLLSISNKIHNKEIEISCDKPFLTTLLFIKKGTSTIHVSNRVINSGPGGKTI
                     EKVDSLCFAQAGKCNYEGSLWAALVLYSLGYDEEVLRFTPYLITMKDEISNQIYIPES
                     FLYYVTGKFQIDLLQLQRLDSYWEESGNKYYDTAIALLPLSGNNLPQKSSSKSWLLSV
                     QDKNGCWNNGNIRDTAFLLYSVWSKNPLSPVGRCDYYYDCPDIDCKSKSCVNGKCVYE
                     SDGSCVYAKCTKDSQCDEYDSTSKEKICGSKNTVYREKYDYSCNLAKGICEAEVTDEV
                     VETCSDSKRCEDGKCIQDSCIVSLFCDEGYKCVKGTCIKTGGSCTDDSDCSDYEYYGT
                     PYCSKDKSAVYQDYYPYYCDTKKELCSPKTKIEEELEVCGTSKECYEGTCLIIECSDS
                     NPCESGYKCHEGSCVPETVCYTKSDCPKKECYEVSCNSGTCLYSYVCDDKECNEDKDC
                     SHMNTEGITFCGGKDDNDVYIEVFEHTCKDNLCVSSTTEKLVKECDDATEECNWGVCE
                     EADNKNYCSGDIDCYGYEYCDVSGVCSPRSCIDDYDCYGGVCSTSGYCESPDSCEDAG
                     YYCTTPSTCKGNIMQEYSCNKGILKCCDTEPPLMPCEDLGGKICSEEQSCVGGAEMRS
                     SDDLDYGETCCVGGICDEGGSTEDINCIDKGGECRSSDSCKKGESEKDYICGDNQICC
                     IKDNDKPKKGWVLIVILLILIFLVVIGIVFRDKLRTEWIKLKDKMSGKKSKKKPDMPM
                     TMQPTSQGRILPRRILPPGQQPSGPPQQTRFPMRTMGQPISGQSPAQPLPVPKPTFTG
                     QTKSSTPQPSTPKKAEEKSKGELDDVLKKLREMEK"
     gene            complement(7881..9440)
                     /locus_tag="PLU55_01800"
     CDS             complement(7881..9440)
                     /locus_tag="PLU55_01800"
                     /inference="COORDINATES: protein motif:HMM:NF016030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86827.1"
                     /translation="MESKKISFKEERIRKITTLYYSQPEIQKAIFEFSKNREICPRYF
                     EGFGKRPDSFEYPGDIFELVKRGATSFNCSEELWSDPLKIQTGMNEKQLNELRIGWDL
                     LIDIDCKYIDFSKKATQAILKVFKNHHIKNYGIKFSGNKGFHIILPFKSFPKEIAGEK
                     TKDLFPELPRKILAYVRFKAEEEMRDLISEEELEAFKDTKIKKGIKCNNCKEIAQEYI
                     LTEYLCPKCFRRELKKIFHNEKKEFKCPDCRTQFKINRADKIYECKKCNISSKDKPDN
                     FSRHIEIDLFDLMGLDLILISPRHLFRMPYSLHENSGLSSIVLSEDELEKFDLKDASP
                     LNLKVKNFMPKCKENEADYLVREALDWTKNNQISSGESKEILKGKYADFKPIILESIK
                     DEDFPPCIKNILKGISDGKKRGLFALINFFRSVGMNKEDMEKRIYEWNKKNEVPLREG
                     YLQTQLLWAYRKKPIMPPNCKEFYQGFGACQPNEFCNLIKNPVNYLVRKNYISNNKKE
                     KKITKKINKNN"
     gene            9526..10245
                     /gene="pcn"
                     /locus_tag="PLU55_01805"
     CDS             9526..10245
                     /gene="pcn"
                     /locus_tag="PLU55_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030337 - DNA polymerase processivity
                     factor activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proliferating cell nuclear antigen (pcna)"
                     /protein_id="HPJ86828.1"
                     /translation="MKIKLDNATILSKSIDLISELVTEVKIKINEFGLSITAIDPANV
                     AMVSLKIPKKAFSEFESGNESLGVNLDSLKRILKRSGGQLVLERKENLLYIQMDDRIR
                     RNFVLSLINIESEDKEIPNLEYSARVELKSQDLIDCVEDCAVVADACSFIVKDEKFVI
                     EAKGLNSTRTEFSSDEANINAETCKARYSLEYLQKFVKASKLTDKTILNFANDHPLKM
                     DVRTESMELSYILAPRVETED"
     gene            10310..11152
                     /locus_tag="PLU55_01810"
     CDS             10310..11152
                     /locus_tag="PLU55_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86829.1"
                     /translation="MSLKEGERGKLKIFPENFRGQVTIFIIIAIIIVAFGVLVYLYYP
                     KIFPNSGGGSKNPALFIQDCLEEDIEDNLKIISSQGGNYVMNKNSGYFYKKNYEDEGK
                     YVRYLCYTDDNYNVPCVNQEPFLKEHIEQEVLDSINESIKTCFDNMVKDYESKGYRTN
                     LKRGKTNIQIIPGEVYTDFNSTLTLTKGDESETYRNFEIRMKSSFYEIIEVSKNILIW
                     EMNIGDSIPEAYMYNNPYIRVEKHRKDNDIKIYVVSEIKSGEDFRFAVRSFASPVGFG
                     DINI"
     gene            11154..13451
                     /locus_tag="PLU55_01815"
     CDS             11154..13451
                     /locus_tag="PLU55_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86830.1"
                     /translation="MKKKCVITVFALALVLFGSMYVSAICCEKLKESEMWCKAATSQS
                     QCDSSYQIWLYKETCNLVPECYGTCVNPKTGECSQYTPKAQCVDSGGVWSEKDIDEVP
                     ACQEYCCVLGQNAYFMTETECKNLMSQHGIQGIIRKDISSRLECEAMQTAVKRGACVV
                     SSLNEKVCNITTNIECTPEKVNELSKYLQDPPTGVNQIDVRFYENHLCTASINGVSIS
                     NCAKSKNTVCKDNKVYYIDLCGNLANIYDSSKYDNVDYWNYMIEEYDDSLCKVGARGS
                     TTCGNCDTTANTVCQNYKDVEKSALPNKPQNNEDGFVCGDLSCKYKGRTYQHGESWCE
                     GTAGTLIINRNLTTGAIFKKDRDELKNASKYNLPGSRYYKLVCSFGEVIVEECGDYRN
                     SVCIQGKDDYSKRSIASCVYNPWRTCSVIDRRSQCESNNTLCKWIPGYRWDLQISSEE
                     ERKEVQGSCVPLIAPGFDFWNPTTQGNTICTSATVRETAFFEVRWHKSRENMGNRENG
                     WGNKGLAHWCLNGCYALPYYGMEFNQIAGEEKKYPEEIECGFRGRGCTSYELLTEFYD
                     KYQIALPKKISDYHLSLRDGQYCHKDGKPDEWSTGKINYVEGSYNTVYDCTGGLGGKQ
                     KSLDKMRDYPIYLTNDEWIKSITERGKSMGDCGYKASINGRYSDPETEMITSIFQKLK
                     QSGEVKENVTVEQIIYKGNAYVQGDLQRYETEMATSVVSYSCMDTPGATCMPAESSEN
                     PCEGGEAGTGTCPTNMVCCVFPELQ"
     gene            13366..>15941
                     /locus_tag="PLU55_01820"
     CDS             13366..>15941
                     /locus_tag="PLU55_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86831.1"
                     /translation="MKILVKGEKLEQEHALQIWFVVCSQNYNKLKIKMKKEKQKKETK
                     AIAILQIFILVIATIAFSWIVGSSIKTVSANPGDDEKKTPPTNSQTDNRPLCGSGGKS
                     FQSCTTLSDCKTIDYTQKCLNYGEYCCAVKKYSIDEGKKDSLFSKAEDIALNIGEGYA
                     TQKAYSFLDNKVFGKKAGEKVVQKAVEEGVSSGTKPIWEFADPGIKPGPHSTPVKPGS
                     ISSWLTGKGGGGGYFGGGGGMVDLFQNLVSGAIWATAAVIFVNYFAKWVGMSDRNSKD
                     IVNAAIAGGTLGVITVVVIGASSSGPIGWIAGAIALAIAALWTGIAYKKYAQEAYTYR
                     IALWQPPTGGAECSKCNLLKVGTGKNQVSGCSEYICHSYGASCEWVNDKTEYETCIEV
                     NKGDISPPVITPAKEIYGEPVFKDNKFKYVSSAAGTRIVYEGEGGGAAGCVPAFTQIK
                     LALITNEEAHCKISLKSSDETAANAFGAMQDMAEGNVYTKNHTLQIPSSILADKESFE
                     NAGYILNNGGKYNFYVRCKDKRENINTQDYEIRFCVQKDLDINPPKIVGTNPAKDSFI
                     PFNVSVIENFQVYTDRPADCKWDIKEASYNYMSYDFDKCSQNVDDYLIGFDYGCQTNL
                     TGFKDGVENKYYVACKNYPEFKGNPEKESKRTPSRPYEIVLKGTNKLVIQSVRINDRA
                     NGTTIIGREENAKIELSVVTIGGAEEGKSRCLYSENQVTPIYSLFSNGRSREYLNTNF
                     EEFYMPQGFYEYLIKCYDSAENTATTLINFTVEVDLLPPIVVRAFKDDSSNSLKIITD
                     ENAKCVYSTTSCNYNVEEGNELSTENGKEHYIEWNPEIDLYVKCIDDYHNYPNEGECS
                     IVV"
BASE COUNT         5785 a         2083 c         2723 g         5250 t
ORIGIN      
        1 tatttttaat aatcatggga cgagaagagc aaataataag tgaaagaata aggaaacttg
       61 aagagttaag aaaagaggga attaatcctt atccaaacaa atatgacaaa aaacaaactt
      121 gccttgaatg ttctaaaagt aagattaaaa gcaaagtcaa aacagcagga agattaatga
      181 ctaaaagaga tttaggtaaa atagctttct gtgttttaag agatggaact ggagatatac
      241 aattagttat tcaggaaaaa gagacacctg aaaagttcag agaatttttt aagaaatatt
      301 tagattctgg agattttgtt ggggttgaag gagaaataat aaaaacaaag acaggagaaa
      361 tatccatact tgtaaaaaag atagagatta tctcaaaatc aatattacct ttacctgaaa
      421 aatggaaagg attacaagat gaagaagaaa gatatagaaa aagatatttg gatattttaa
      481 tgaacaaaga tgttaaagag atgtttgtta aaaaagcaaa attctggaat tcaataagga
      541 attttcttct tgaaaaagga tttttagaag ttgaaactcc tgttcttgag agttctgctg
      601 gaggagcagc tgcaaaacca tttataactc atcataatgc tttagattta gatgtttatt
      661 tgagaatttc aatgggagaa ttatggcaga aaaaattaat ggttgcagga tatgaaaaaa
      721 catttgagat aggaagacaa ttcagaaatg aaggaatgga tatggaacat ttacaggatt
      781 acactcaaat ggaattttac tggggttatg caaattatca agatggaatg aaacttgttg
      841 aagagatgta taagaaagtt gcaaaagaag tatttggaac aatgaaattt gatatacatg
      901 ggtataaagt tgatttctct aaaaaatggg aaatttatga ttttgaaaca ctcataaaaa
      961 aagaaacaaa aatagatatt tacaaggcag atgtaaatga aataaagaaa aaactaaatg
     1021 aattaaaaat agaatataat ccttcagttg ataaatggag acttgtggat cagctttgga
     1081 aatattgtag aaaaaaaata tctggacctg gatttttaac agggcaacct gttgaactct
     1141 ctcctcttgc aaagagaaac ccagaagata aaagaaaggt tgaacaattt caagttataa
     1201 ttgctggaag tgaggtagga aacggatatt cagaattgaa tgatccttta gaccaagaag
     1261 aaagatttaa agaacaatca aaacaaaaag aagcaggaga tgaagagagt atggaacatg
     1321 ataaaggatt tgttgaagct ttgaaatatg gtatgcctcc tacatgtgga tttggattaa
     1381 gtgaaagatt gtttgcattt ttaatgggaa aaacattaag agagtgtgtt attttccctt
     1441 taatgaaacc tgaaataaat aaagagaaat aaaaatggga ttaataataa aatcaaatat
     1501 acgtccaaga gttaaagaac tagataaaga aaacgtaata tcttctgttg cagatgaagt
     1561 tgaaattgct cttgaaagaa aagttgaaga gatattagat gaagcaataa aaagagcaaa
     1621 ggcaaatggg aggagaactc ttcaagcaag agacttgtga tataaataat tatgataaat
     1681 cctgttttct tttatatttt gggaataatt atatttgttt tacttttaat ctttggactt
     1741 aaattctcaa aatacaagat aaaatatata aagtatggag gatatctttt aattataata
     1801 gggttattag gattagttat tgatttatac aatatattca ataaatacct aatgccttca
     1861 ataataaaca agtaaaatgg cagttcgtag atatcgtaga acaacttaca ggagaagatt
     1921 tcctgtgctt ggatttataa ttctaatatt tgcaacttta tggctattaa gagaaatgga
     1981 tattattgat ttatatgttc cttggttacc agttgtttta attataatag caattggaat
     2041 tatttttaat agattgattg gataaaaaaa ttaactagta gattagagca acttagatgt
     2101 tttttctaat atgcgttatt taccatttca cacaatttgc tttgataaga atctttgtgt
     2161 ttttgtttca tcttgtaaag cattgctatg atatcactat ttctttcatc ccttgttttt
     2221 atctgatggt ttaaatcaga tattaatttt tttagattat ctgttgtcaa tttagaacct
     2281 cttttgatgc cataatctat ttccataata tccattattc ttttagaaat aacttgctgt
     2341 tctggtgctt tacttgccca tttccccaat gtttctacag ggattaaata atctattact
     2401 cttttattat ttattttagg gtatctccta taattgcttg taaatacctt gattaaatca
     2461 tccatagttt caagagcttg ttctgaaaca gcatgtttta tctcatggtc gatagcattt
     2521 aaagtgcgat atctgctacc atctttaaat atattaataa ctggattttt tttgtcttga
     2581 taataaaccc cttttgctaa atcatctttt ttatttgaaa tatttagttt tgtttttgca
     2641 agttgttctt taatcttttg gatatctttt tttatcttgc tttctgacat tccagttgct
     2701 tctttttttc tagaaatata ttcattgctg tctaaccact ttaattctct ttcaataaaa
     2761 tagtctattt tttgtgattg ttcactatct gcattattga ttttttttag ataaattggt
     2821 gaattaggca gataagtatc acaagaactt ttagaattcc atttattgaa atcagttctt
     2881 gattcaattg gttgtcctaa tatatctgta agctctctca taaatatact acacgttgct
     2941 agtatataag tctttctatt tgttaccact tgttagatac taaacaatac tatttaataa
     3001 gacataatca aaattattta gatactctga attaaaaaca acagaaatat atacatggga
     3061 ttctacaaaa gatttatcaa aaataaaact ctataaataa attgtaaaat ggtagccaaa
     3121 aaaggagttg atataaaaaa acagactaaa tcagcacatt caagtcaggg taagaaaaat
     3181 cgtgctgcag gaagaaggtt tgaagctaaa gtacgtgaga atctagagga aatgggttgg
     3241 attgtaaata aatggatgaa taatattgac tatgaaaaaa acaagatagt tcctgcaaaa
     3301 agaaaataca atcctttttt gaaagctttg agtattggaa cagggttccc agattttgtc
     3361 tgttttaaaa aagatgaaac tggaagatat gaagttattg ggctggaagt taaaagtaac
     3421 gggtatttgg accaatctga aaaagggatg tgtttgtggc ttattgaaaa taaaattttc
     3481 tcaagaatat taattgcaaa aaagaatatg aaaggaagaa aaatagaaat agaatatatt
     3541 gacttctctg aaaagtataa ttcaaaacaa cagaatacag aacaataatc cttaaaacaa
     3601 actttattta attatttaaa tgagaaagaa atttcttttt ttcattgtat ttttcatatt
     3661 ttcaattata aattttagct tgatatctgc ttcctgtcaa aagaatcaaa ttaatattaa
     3721 tactgcttca ttagaagagt tggacaaact tgcaggaata ggtcctgtta aagcacaggc
     3781 aataattaat tctcgtcctt tcaataaatt agatgacttg ttaaatgttg ttgggatagg
     3841 tgatgttaca ctttctaata taaaacagca aggacttgct tgtgtaagtg gtgaagagga
     3901 agaggaggaa gaacaagaag ataatgaaga agaggatgaa gaaaaggaag ataaaaaaga
     3961 aacaataaaa caaataaaaa aaattnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnagagg atgaagaaaa ggaagataaa aaagaaacaa taaaacaaat aaaaaaaatt
     4141 agtgtaaatc ctgaagaaaa taaaatacaa gaggaacaag aaataaagcc aataatactc
     4201 tctccaatat ctccaaaaag tataaaaaca gaaccgtaca aaaaagaatt agacaagaac
     4261 aaatttgcaa tttatgggct agtattttta ggcattttgg tttttttctt gtttaaaatg
     4321 aagaaacaaa aaaatgaaaa aacagagttc aaataaagaa gattcaggaa aaaaagtaat
     4381 tagttctaca atcatacttg aaataatagg aagaccagca gaacaacttg taactacatt
     4441 agaaggttta ataaaaaaga tgggagaaga aaaaggagtt aaagtaacta acaaaaagat
     4501 aaatgaacct gtattaataa aagacacaaa agatttttac actacttttg ctgaagttga
     4561 tttagaagtt gaagatattt tatatttagc gattataatg tttaagtata tgcctgcaca
     4621 tgttgaagtt gttgaaccag aattgatagc tcttacaaat aatggttgga cagatatatt
     4681 aagtgaatta acaagaaggc ttcatggata tgaagaagtt gcaagagttt tacagtttca
     4741 aaattcagag atgcaaaaaa agcttaaaga attattacca gaaaataaag accaagaaaa
     4801 aaagaaataa aatctatttt tccatttctc tcaatttctt taaaacatca tctaattcac
     4861 ctttagattt ttcttctgct tttttaggtg tagaaggttg aggtgtagaa gatttagttt
     4921 gtcctgtaaa agttggttta ggtactggaa ggggttgggc tggagattgt cctgaaatag
     4981 gctgtcccat tgttctcata gggaatctgg tttgttgagg aggtcctgaa ggctgttgcc
     5041 ctggaggtaa aattcttcta ggtaaaattc ttccttgaga agttggttgc attgtcatag
     5101 gcatatcagg ctttttcttt gattttttcc cactcatctt atcttttaat tttatccatt
     5161 cagttctcaa tttatctctg aaaactattc ctataacaac taaaaatatt aaaatcagca
     5221 agataactat caacacccat ccttttttag gtttatcatt atccttaata caacaaattt
     5281 gattatcccc gcaaatataa tctttttcag attctccctt tttacaagaa tcagaggacc
     5341 tgcattctcc ccctttatct atacaattga tatcttctgt tgaacctcct tcatcgcata
     5401 ttcctccgac acaacaggtt tcaccataat ctaggtcatc tgatgatctc atttctgctc
     5461 caccaacaca agattgttct tcagaacata tttttccacc caaatcttca caaggcatta
     5521 aagggggttc tgtatcacaa cactttaaaa tacctttatt acaagaatat tcctgcatta
     5581 tatttccctt acaagttgat ggagtggtac aataatatcc tgcatcttcg caactatcag
     5641 gagattcaca atatccgctt gttgaacata ctcctccata acaatcataa tcatctatgc
     5701 aagacctagg agaacaaact ccagaaacat cacagtattc ataaccataa cagtcaatat
     5761 cgccactaca ataattttta ttatccgcct cttcgcatac tccccaatta cactcttctg
     5821 ttgcatcgtc acattctttt actaattttt ctgttgtact tgaaacgcaa agattatctt
     5881 tacaagtgtg ctcaaaaacc tcaatataaa catcattatc atctttccct ccacaaaatg
     5941 ttattccttc tgtattcatg tgagaacagt ccttatcctc attacactct ttatcatcgc
     6001 aaacataaga gtaaaggcag gttccagaat tacaagaaac ctcataacat tctttcttag
     6061 gacaatcact cttagtgtaa cagactgttt caggtacaca gcttccttca tggcatttgt
     6121 aacctgattc gcaaggattt gaatcagaac attctataat taaacaagtt ccttcataac
     6181 attcttttga tgtaccacaa acttcaagct cctcttctat tttagtttta ggagaacata
     6241 actctttttt tgtatcgcaa taataagggt aataatcttg ataaacagca gatttatctt
     6301 tactacaata tggagttcca taatattcat aatctgaaca atcagaatca tcagtacacg
     6361 aacctcctgt tttaatacaa gttcctttta cacatttata tccctcatca caaaaaagag
     6421 atactataca actatcttga atacattttc catcttcaca tctttttgaa tcagaacaag
     6481 tttcaactac ttcatctgtt acctctgctt cacatattcc cttagcgaga ttacaagaat
     6541 aatcatattt ttcgcgataa acagtatttt tgcttccgca aattttttct tttgaagtag
     6601 aatcatattc atcacattga gaatcttttg tacatttagc ataaacacaa cttccatcag
     6661 attcatagac gcatttccca tttacacaac ttttagattt acaatctatg tctggacaat
     6721 cataataata atcacaacgt ccaacaggag aaaggggatt ttttgaccat actgaataaa
     6781 gtaaaaatgc agtatctctt atatttccat tgttccaaca tccattttta tcttgtacag
     6841 ataaaagcca ggattttgaa ctagattttt gaggtaggtt attaccagag agaggaagta
     6901 atgcaatggc agtgtcataa tatttatttc ctgattcttc ccaatacgag tcaagtcttt
     6961 gtagctgaag aaggtcaatt tggaattttc ctgtgacata atataaaaaa gattctggga
     7021 tataaatttg gttagatatt tcgtctttca ttgtgattaa ataaggggtg aatcttaaaa
     7081 cttcttcatc ataacccaaa gaatataaaa caagagcagc ccataaactt ccttcataat
     7141 tacactttcc tgcttgtgca aaacaaagcg aatcaacttt ttctattgtt tttccacctg
     7201 gtcctgaatt aataactcta tttgaaacat gaattgtaga agtacctttt tttataaaaa
     7261 gaagtgttgt taaaaatggt ttatcacaag aaatttcaat ttctttgttg tgtatcttat
     7321 tagaaatgct taataaatat ccgtcttgtg ctaaagtcaa atgatttcct gcattagaag
     7381 aaatttttct atcttcccct attgtaactt tataagttgc tccagaataa gaaatatcac
     7441 ataaagtagc tcctgattta gattctattt ccaaaaacca ttcaatattt tcaggtgatg
     7501 ttgtttgttt taaaagccag ttttcagctt tttttgtatc tgctctggaa tttaaagcca
     7561 atattgcctg cgcagtggat tttaatgtac atcctgattt tggccaacac tcattagaac
     7621 gattttctaa agataattca tttccacagt ttcctgaagc tagataagaa aaaacttttt
     7681 cttcaaattt aagagcagaa catcctgttg aattaattct tttatcaaga caggtataag
     7741 cagattcaag accttttaat tcatctgctg cattaatagt tgataaagga attagaaata
     7801 atgtaaaaac taacaacaat gtcacttctc ttttcatatg ttctgaagca atttcttatt
     7861 tataaatatt tttataaaaa ttaattattc ttatttatct ttttagttat tttcttttct
     7921 tttttattgt ttgaaatata attttttctt accaaatagt ttactggatt tttaattagg
     7981 ttgcaaaatt catttggttg acatgctcca aatccttgat aaaattcttt gcaattagga
     8041 ggcattattg gtttttttct gtatgcccat aagagttggg tttgtagata tccctctctt
     8101 agaggaacct catttttctt gttccactcg taaattcttt tttccatatc ttctttattc
     8161 ataccaacag aacgaaaaaa gtttattaaa gcaaataatc ctctcttttt accgtctgaa
     8221 attcctttca ggatattttt tatgcatgga ggaaaatctt catctttaat tgattctaaa
     8281 attattggtt taaaatctgc atatttcccc ttaagaattt cttttgattc tcccgaggat
     8341 atttgattat tttttgtcca atccaaggcc tctctcacaa gataatctgc ttcgttttct
     8401 ttgcattttg gcataaaatt ttttactttt aaatttagag gagatgcatc tttcaagtca
     8461 aatttttcta attcatcttc gcttaaaaca atgctggata aaccactatt ttcgtgaaga
     8521 gagtaaggca ttctgaataa atgtcttgga gagattaaaa ttaaatccaa ccccattaaa
     8581 tcaaacaaat ctatttcaat atgtcttgag aaattatctg gtttatcttt agaagaaata
     8641 ttgcattttt tacattcata aatcttgtct gctctgttta ttttaaattg cgttctgcaa
     8701 tcaggacact taaattcttt tttttcgtta tgaaatattt tttttagttc tctcctaaaa
     8761 cattttgggc aaagatattc tgttaatata tattcctgag caatctcttt gcaattgttg
     8821 cattttattc ctttttttat ttttgtgtct ttaaatgcct caagttcttc ttcagaaatc
     8881 aaatctctca tctcttcttc tgctttaaat cttacataag ctaaaatctt tcgtggtaat
     8941 tctggaaaga ggtcttttgt tttttctcct gcaatctctt tgggaaatga cttgaatggg
     9001 agaataatat gaaatccttt attccctgaa aatttaattc cataattttt tatgtggtga
     9061 tttttgaaaa ctttgagtat tgcctgtgtt gcttttttac tgaaatcaat atatttacag
     9121 tcaatgtcta tgagtaaatc ccatcctatt cttaattcat ttaattgctt ttcattcata
     9181 cctgtttgta ttttaagagg gtctgaccac aattcttcag aacagttaaa ggaagttgct
     9241 ccgcgtttta caagttcaaa tatatctccg ggatattcaa aagaatctgg tctttttcca
     9301 aatccctcaa aatatctcgg acaaatctcc ctattttttg aaaactcaaa aatagctttt
     9361 tgaatttctg gttgagaata gtatagtgta gttattttcc ttattctttc ctctttaaat
     9421 gaaatttttt ttgattccat tgttttataa gtgggaaagg attttaataa ttatcttata
     9481 cttaagaata gcaatgctta aaaaacagga gtatctttta tgattatgaa aattaaactt
     9541 gataatgcaa ctatattatc taaatccatt gatttaatct ctgaattagt aacagaggta
     9601 aaaataaaaa taaatgaatt tggactgagt ataactgcca tagaccctgc aaatgttgca
     9661 atggtaagtt tgaaaatacc aaaaaaagct ttttctgaat ttgagagcgg aaatgaaagt
     9721 ttgggtgtaa atttagatag tcttaaaaga atattgaaaa gaagtggagg acagcttgtt
     9781 cttgaaagaa aagaaaattt actttatata caaatggatg acaggataag aagaaatttt
     9841 gttttaagtt taattaatat tgaaagtgaa gacaaagaaa tacctaattt agaatatagt
     9901 gcaagagttg aattgaaatc tcaggattta attgattgtg ttgaagattg tgcagttgtt
     9961 gctgatgctt gctcatttat tgtgaaagac gaaaaatttg tgattgaagc taaaggactt
    10021 aattcaacaa gaacagagtt ttcaagtgat gaagctaata taaatgcaga aacatgcaag
    10081 gcaagataca gcttagagta tcttcagaaa tttgtaaaag catctaaatt aactgataaa
    10141 accattttga attttgctaa tgaccatcca ttaaaaatgg atgttagaac agaaagtatg
    10201 gaattgtcct atattcttgc tccaagagtt gaaactgaag attaaataaa tttatttaat
    10261 ataatttaaa ttaagaaatg tttttaaaca actaaaacat taaaaaaaga tgagtttaaa
    10321 agagggagaa agaggcaaac taaaaatttt tccagaaaat tttagaggcc aagttactat
    10381 ttttataata attgcaataa tcattgttgc atttggggta ttagtttatt tatattatcc
    10441 aaaaatattc cctaattcag ggggtggatc aaaaaatcct gcattattta tccaggattg
    10501 tcttgaagaa gatattgaag ataatttaaa aataataagt tctcaaggag gaaattatgt
    10561 tatgaataaa aatagtggtt atttttacaa aaaaaattat gaagatgaag gtaaatatgt
    10621 gagatacctt tgttatactg acgataatta caatgttcct tgtgtcaatc aagaaccatt
    10681 tttaaaagaa catattgaac aggaagtatt agattctata aatgaaagca ttaaaacatg
    10741 ttttgataat atggttaaag attatgaatc caagggttat cgtactaatt taaagagggg
    10801 aaaaacaaat attcaaataa ttccaggaga agtttatact gattttaata gcacacttac
    10861 attaacaaaa ggagatgaga gtgaaaccta cagaaatttt gagataagaa tgaaaagtag
    10921 cttttatgaa ataattgaag tttctaagaa tatacttatt tgggaaatga atataggaga
    10981 ttcgatacct gaagcatata tgtataataa tccttatatt agagttgaaa aacacagaaa
    11041 agacaatgat ataaaaattt atgtggtatc tgaaataaaa agtggagagg atttcagatt
    11101 tgcagtaaga agctttgctt ctcctgttgg atttggagat ataaatattt aagatgaaaa
    11161 aaaagtgtgt gataacagtt tttgctttgg cattagtttt atttgggagt atgtatgttt
    11221 ctgctatttg ttgtgagaaa ctaaaggaga gtgaaatgtg gtgtaaagct gcaacaagtc
    11281 aaagtcaatg tgactcatca tatcagatat ggttatataa agagacatgt aatttagttc
    11341 cagaatgtta tggaacatgt gttaatccaa agacagggga gtgtagtcaa tatactccta
    11401 aagcgcaatg tgttgattct ggaggagttt ggagtgaaaa agatatagat gaagttccag
    11461 catgtcagga gtattgctgt gttttaggac aaaatgctta ttttatgaca gaaactgaat
    11521 gtaaaaattt aatgtctcaa catggaattc aagggataat aagaaaagat attagttctc
    11581 gactagaatg tgaagctatg caaacagctg ttaaaagagg ggcttgtgtt gtgagttctt
    11641 taaatgaaaa agtgtgtaat ataaccacaa atattgaatg tactcctgaa aaagtaaatg
    11701 aactttccaa atatttgcaa gatcccccta caggagttaa tcaaatagat gtaagatttt
    11761 atgaaaacca tttatgcact gcttcaataa atggtgtttc tatttcaaat tgtgctaaaa
    11821 gtaaaaatac cgtgtgtaaa gacaataaag tttattacat agatttgtgt ggaaatttag
    11881 ctaatattta tgattctagc aaatatgata atgttgatta ttggaattat atgatagaag
    11941 aatatgatga ttctttgtgt aaagtgggtg ctcgtggaag tacaacttgt ggaaattgcg
    12001 atacaactgc aaatacagtt tgccagaatt acaaagatgt tgagaaaagt gctttaccta
    12061 ataagcccca aaataatgaa gatgggtttg tgtgtggaga tttaagttgc aaatataaag
    12121 gaagaacata tcaacacgga gagagctggt gtgaaggaac tgcaggaaca ttaataatta
    12181 atagaaattt aactacagga gcaattttca aaaaagatag agatgaatta aaaaatgcaa
    12241 gcaagtataa tttacctgga agcagatact ataaattagt gtgttctttt ggggaagtta
    12301 ttgttgaaga gtgtggagat tatagaaata gtgtgtgtat acaagggaaa gatgattatt
    12361 caaaaagaag tattgcttct tgtgtatata atccttggag aacttgttct gttatagata
    12421 ggagaagtca atgtgaatca aacaacactt tatgtaaatg gattcctgga tataggtggg
    12481 atttacaaat ttcttctgag gaagaaagaa aagaagttca aggttcatgt gttcctttaa
    12541 ttgctcctgg ttttgatttt tggaacccta caacccaagg aaatactata tgtacatctg
    12601 caactgttcg agaaactgct ttttttgaag ttcgttggca taagagcaga gagaatatgg
    12661 gaaacagaga aaatggttgg ggaaataaag ggcttgctca ttggtgttta aatggatgtt
    12721 atgctcttcc ttattatgga atggaattca atcaaatagc aggggaagaa aaaaaatatc
    12781 ctgaagaaat agaatgtgga tttagaggaa gagggtgtac ttcttatgaa ttattaactg
    12841 aattttatga taaatatcaa atagcattac caaagaaaat ttcagattat catttatctt
    12901 taagagatgg ccaatactgt cataaagacg gtaaaccaga tgagtggtca actggaaaaa
    12961 taaattatgt agagggttct tataatacag tttatgattg cactggggga ttaggaggta
    13021 aacaaaaaag tttagataag atgagagatt acccaatata cttaacaaat gatgaatgga
    13081 ttaaatcaat aactgaaaga ggtaaatcta tgggagattg tggatataaa gcaagtataa
    13141 atgggagata tagtgaccct gaaacagaaa tgataacttc tatatttcaa aaattaaaac
    13201 agagtggaga agttaaagaa aacgttacag ttgaacaaat aatctataaa ggaaatgcat
    13261 atgtccaagg agatttacaa aggtacgaaa ctgaaatggc tacaagtgta gtaagttatt
    13321 catgtatgga tactcctgga gcaacatgta tgcctgcaga aagtagtgaa aatccttgtg
    13381 aagggggaga agctggaaca ggaacatgcc ctacaaatat ggtttgttgt gtgttcccag
    13441 aattacaata aactaaaaat aaaaatgaaa aaagaaaaac aaaaaaagga aacaaaggca
    13501 attgcgattt tgcagatatt tatattggta attgcaacaa ttgcattttc atggattgtt
    13561 ggaagttcaa ttaaaacagt gagtgcaaat cctggtgatg atgagaagaa aactcctcct
    13621 acaaatagtc aaacggataa tcgtccttta tgtggttctg gaggaaaatc attccaatct
    13681 tgtactacat tatctgactg taaaacaata gattatactc agaaatgttt aaattatgga
    13741 gaatattgtt gtgctgttaa gaaatatagt atagatgaag gaaaaaaaga tagtttattt
    13801 agtaaagcag aggatatagc actaaatata ggagaaggtt atgctactca aaaagcttat
    13861 tcattccttg ataacaaagt atttggaaaa aaagctggtg aaaaggtcgt tcaaaaagca
    13921 gttgaagaag gggtatcttc tggtacaaaa cctatttggg aatttgctga tccaggaatt
    13981 aaaccaggcc cacattcaac ccctgtaaag ccaggttcaa tctctagttg gttaacaggt
    14041 aaaggaggtg gcggaggata ttttggaggt ggcggaggaa tggtggattt atttcaaaat
    14101 ttagtttctg gagctatctg ggctactgct gctgttattt ttgttaatta ttttgctaag
    14161 tgggtaggta tgagcgatag gaattctaaa gatatagtaa atgcagcgat tgctggagga
    14221 acattggggg taattacagt tgttgtaatt ggagcatctt catcaggacc aataggatgg
    14281 attgctgggg ctattgctct agcgatagct gctctttgga caggaattgc atataaaaaa
    14341 tatgcgcagg aagcttatac ttatagaatt gcattatggc aacctccaac aggaggagct
    14401 gaatgtagta aatgtaattt attaaaagtt ggaacaggaa aaaatcaagt gagtggatgt
    14461 agcgaatata tttgccattc atatggtgca tcttgtgaat gggtaaatga taaaacagaa
    14521 tatgaaactt gtattgaagt aaataaagga gatatttctc cacctgtaat aacacctgca
    14581 aaagaaattt atggtgaacc tgttttcaaa gataataaat ttaaatatgt tagttctgct
    14641 gcaggaacaa gaattgttta tgaaggagag ggtggaggag ctgcaggatg tgtccctgct
    14701 ttcacccaaa ttaaattagc tttaattaca aacgaagaag ctcattgtaa aataagtctt
    14761 aaatcaagtg atgaaacagc agcaaatgca ttcggtgcaa tgcaagatat ggctgaagga
    14821 aatgtttata caaagaatca tacattacaa attccttcta gtattttagc agataaagaa
    14881 tcctttgaaa atgcaggata tatattaaat aatggaggaa aatataactt ttatgtgaga
    14941 tgtaaagata aaagagaaaa cattaacact caagattatg aaatacgatt ctgtgtacaa
    15001 aaagatttag atataaatcc tcctaaaata gtaggaacaa accctgctaa agattctttt
    15061 atcccattca atgtaagtgt tatagaaaat ttccaagttt atacagatag gcctgcagat
    15121 tgtaagtggg atattaagga agcaagttat aattacatga gttacgactt tgataagtgt
    15181 tctcaaaatg tcgacgatta tttaattgga tttgattatg gatgtcaaac taatttaact
    15241 ggatttaagg acggtgttga aaataagtat tatgttgcat gtaaaaatta tccagaattt
    15301 aaaggaaatc ctgaaaaaga aagtaaaaga actccttcaa gaccttatga aatagttttg
    15361 aaaggaacaa acaaattagt tatacagagt gtaagaataa atgatagagc aaatggtaca
    15421 actataatag gaagagaaga gaatgcaaag atagagcttt cagttgtaac tattggagga
    15481 gcagaagaag gtaaatctag atgtttatat tctgaaaatc aagttactcc tatatattct
    15541 cttttcagta atggtagaag tagagaatac cttaatacaa actttgaaga attttatatg
    15601 cctcaaggat tttatgaata tttaataaaa tgttatgatt ctgctgaaaa cactgcaaca
    15661 acattgataa atttcactgt agaagtagat ttacttcctc caatagttgt gagagcattt
    15721 aaagacgata gttctaattc tcttaagatt ataactgatg agaatgctaa atgtgtatat
    15781 tctacaactt cttgtaatta taatgtagaa gaaggaaatg aattaagcac agaaaatgga
    15841 aaagagcact atattgaatg gaatccagaa atagatttat atgttaaatg tattgatgac
    15901 tatcataatt atccaaatga aggagaatgt agtatagtag t
//
LOCUS       DAOPCH010000015        13982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_4382_length_13982_cov_5.537445, whole genome shotgun sequence.
ACCESSION   DAOPCH010000015 DAOPCH010000000
VERSION     DAOPCH010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 13982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13982
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4382_length_13982_cov_5.537445"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PLU55_01825"
     CDS             <1..433
                     /locus_tag="PLU55_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86832.1"
                     /translation="GILTLVLPATSPIFCSVVACANVGHTSLNRLSHLVQGSQSIKVQ
                     STKSVAPVASTIALSTCHKFGTCPLCVAVGTIGLFNKVLTQSIIWFPVSFTTLLSIFL
                     PSISGYVLKSFQAQSYSHSFICGFVGRSSALFSSPIFLPCA"
     gene            complement(1689..1931)
                     /locus_tag="PLU55_01830"
     CDS             complement(1689..1931)
                     /locus_tag="PLU55_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86833.1"
                     /translation="MKLLIKVLEHEFIDTVLTTEIIDSEVVIEEDIGTFAVARFSMPL
                     IDIEEYNIVEIYEVENTDTRIFRGYVYKIEPVRNQF"
     gene            1954..2361
                     /locus_tag="PLU55_01835"
     CDS             1954..2361
                     /locus_tag="PLU55_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86834.1"
                     /translation="MSLYVIYTIGVSFRLSVNTAIWFNQGSRNISAQLFLCALIFTPL
                     SVTIKFSNGLLFKFLLSNTRFVVVQFILYLFPFAVLIIFGLVAVQTICILQGAIQFQA
                     FVTGIAQAKLQKVVGSVVLNVQPPINAFVPVIV"
     gene            complement(2933..3256)
                     /locus_tag="PLU55_01840"
     CDS             complement(2933..3256)
                     /locus_tag="PLU55_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86835.1"
                     /translation="MTEEEIEALQLKIDAQKEYDALTEIGKIKADYEAKKAIAEQELL
                     DLQNKLSEEEALFEAYTQAKEQMERDYTEQFKGMIEEQKSAIDGLISKYNALARAKSS
                     AMGSG"
     gene            4047..4988
                     /locus_tag="PLU55_01845"
     CDS             4047..4988
                     /locus_tag="PLU55_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86836.1"
                     /translation="MFLYSSDANFIASANVLKFQEPANIVFLNLNSVIHSSSNIRPAS
                     VQAELIHSNTFTKASQVLFCTNHLNSSAVIQATVANFCNSAHHLAAASFICQKNFTID
                     VPQAAAFIQEATKAVDIHSISVAVKLDNNQAVCASFAKFKISCSLAAKLLPNQTKALH
                     KFSISVIGTHNTSENLFNDVAASSALKTVAIPNQAATSVNFIKLSLHLTHNCQATSAI
                     QANSSKVKENCLDNLLNHFSNISYHSAVSSNVFCTHTKAVSKLLAVFVPFTMALPKAY
                     IEDIAIVIGLTRLFNIQPPKLLNFACNLLTVVSPVFL"
     gene            complement(5659..5943)
                     /locus_tag="PLU55_01850"
     CDS             complement(5659..5943)
                     /locus_tag="PLU55_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86837.1"
                     /translation="MQRTIRKSIEISLIIEKRLLFWKYDKVIKLKFKQATVMEAIDFG
                     ETIKGNMFNIVIWLLDFITKHSDKKITKRDKQCIMVKWGEILDLIKKHYL"
     gene            complement(6188..6313)
                     /locus_tag="PLU55_01855"
     CDS             complement(6188..6313)
                     /locus_tag="PLU55_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86838.1"
                     /translation="MDVLEKVLGISSVDVSGTTDYTAYGSNLQVKELPKVVVKIV"
     gene            complement(8546..8686)
                     /locus_tag="PLU55_01860"
     CDS             complement(8546..8686)
                     /locus_tag="PLU55_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86839.1"
                     /translation="MYFREVKGDVEVSKPNTDIIETSKPETTLDKKSLIKLYIEKFGK
                     RP"
     gene            complement(8890..9393)
                     /locus_tag="PLU55_01865"
     CDS             complement(8890..9393)
                     /locus_tag="PLU55_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86840.1"
                     /translation="MRKTALAGTVNVDYKDLGTLAMEDLFVLRGLMNKYSTNLQDLVL
                     IMDYKAYNKALTISEFLEFQKNGVNSTAITGALSNIAGVDLFVHRDFPSTEADGKASA
                     TASNNTKGSALYVWRPAVQRGYGKNGLDITVYKIPGKGYQFVGTMDAGFAIANKKAGE
                     TDPAVVL"
     gene            complement(9427..10017)
                     /locus_tag="PLU55_01870"
     CDS             complement(9427..10017)
                     /locus_tag="PLU55_01870"
                     /inference="COORDINATES: protein motif:HMM:NF016923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HPJ86841.1"
                     /translation="MDKLLEQLAELKSIEITDEVKELSEKKISDMRAKANEVVHTTNT
                     GYGKELIPVNVLTDTVLEMIPTYAGILNAFMVGFHGNSMGKSEKVPIVGEIPFAQGNS
                     EWTTGAGAIAQGKQLLPTGDVTINQYSMIVSVDVSNEELAYSVADLQTMILRKMSQSF
                     VRTFESAIVNADPETSSVGNVNSDDQAPATTFAATG"
     gene            complement(10193..10360)
                     /locus_tag="PLU55_01875"
     CDS             complement(10193..10360)
                     /locus_tag="PLU55_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86842.1"
                     /translation="MEKRFNQIEKSLETIEETKGLSIETKSQIELLQKDLSDLKSETK
                     KFKEDMSVVVE"
     gene            complement(10433..10960)
                     /locus_tag="PLU55_01880"
     CDS             complement(10433..10960)
                     /locus_tag="PLU55_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86843.1"
                     /translation="MNDEEYAKNFQISKNDEYTLYKSIEDLRIKSEDLVEKEEVVEGK
                     EKIIEEKEEIKEEITEVEEENTDTEEITEVEEENTDTEEKEEENPVDTDTEEEIKEVE
                     KENEDNVGEEEKTDEIETEKEVVEEKSLEIEEKTDEDVSIEEISEITEETESEQNIEE
                     DKETIEEPEIPENVV"
     gene            complement(11198..11335)
                     /locus_tag="PLU55_01885"
                     /pseudo
     CDS             complement(11198..11335)
                     /locus_tag="PLU55_01885"
                     /inference="COORDINATES: protein motif:HMM:NF016471.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
     gene            complement(11874..12143)
                     /locus_tag="PLU55_01890"
     CDS             complement(11874..12143)
                     /locus_tag="PLU55_01890"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HPJ86844.1"
                     /translation="MSDTESSKRQFYFFENNSVPNALFMLDPNITMDKDKLSQIKEDV
                     NNKYKGSENAHKIMIAGGIQDVKILELSNKDLDLINLRNFIVKKW"
     gene            complement(12285..12464)
                     /locus_tag="PLU55_01895"
     CDS             complement(12285..12464)
                     /locus_tag="PLU55_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86845.1"
                     /translation="MKKVYDVFKDNTRSTLKDKYFTNHFCSGDIYLYPRKTLSNDIAC
                     QIVDSRTIIKEVDKF"
     gene            complement(13271..13528)
                     /locus_tag="PLU55_01900"
     CDS             complement(13271..13528)
                     /locus_tag="PLU55_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86846.1"
                     /translation="MFDENIEFDYIEAGIDPAFSEKTKSDSFSITVTGFKKVENVLYR
                     YVLEEISLKGEEKNNQNICRTVLNMYLNYKPRNIKVEKNNG"
BASE COUNT         3846 a         2610 c         1660 g         5866 t
ORIGIN      
        1 tgggatactt actctagtgt tacctgctac atctccaata ttctgttctg ttgttgcttg
       61 tgcaaatgtt ggtcatacct ctttgaatag actatctcat ttagtacaag gttctcagag
      121 cataaaggtt caatcaacta aatctgttgc acctgtcgcc tctacaatag cactttcaac
      181 ttgtcataaa tttgggactt gtccactttg tgtcgctgta ggtactattg gactttttaa
      241 taaagtttta actcaatcta ttatttggtt tcctgtgagt tttactacat tactatctat
      301 ttttttacca tctatttctg ggtatgttct taaatcattc caagctcaat catattccca
      361 tagttttata tgtgggtttg ttggtcgttc atcggctctt tttagttctc caatattctt
      421 accctgtgca taatctgtgc ttggataagt atcctcaatc agtgttgggt catcaatcaa
      481 agtctggtct aattctatat atatctttgt cccagttcca gtcaatgtct ttgtatctga
      541 taattgatac cttactgcta ttttcttgtt agcgataggt gtgcttgtcg cccttgttgc
      601 tacgattata gcatttcaag cacttatagt aacatcacca ctggttcaat tcaaatcaaa
      661 ctcggttttt acatcaacat catcaaataa tacccctcaa gtattatctg tgagtcgtaa
      721 ttcaaaagct gaaaagtcat aatctgaatt aagcagacta tcattactat ctaaaagtga
      781 taccctttcg attgctgtca ttttatctta ttagttataa actaaaattt atatatttat
      841 attgttggat tttacaaact aacttatgtt tttgttttat tttaaacaat ttatttgctt
      901 ttcaataatt ttactgattt ttgtatccca tacaaccagt tttctatatt tatcacatct
      961 aaatctaatt cccccacctt atagcttgtt attttacttc aattagtgta atttattgtc
     1021 ttacctatca catatacatc actatctata tcaaagaaac tgtttgtatt ttctactact
     1081 aatttcattt tatcatatat atttgctatt attgcatttt gttcaacagt tatttcatat
     1141 attctttggt ttttattttg tttttctagt tctttatctg ttttctctat aatatctcat
     1201 tccctaaatc tttcaaaagc tactcaatat aatctatcta cataatcgtt gcttgtatgt
     1261 tttgtgtctt cttgtcattt acctataact atattatttc ttgttgctgt cccaattact
     1321 cttatatttg atatattagc attattaggg aatagtcaat tataatttat ctcttgataa
     1381 tttattcaag aagtcctatc acttcaaaaa tttctcttaa ataatatctt tttatctact
     1441 atatctcgtt gtgcttcaca ttgctcagct agttcatcaa gaattcaaaa ataatcatct
     1501 cattcattta gttttaagtt aattgtttta cttaaatcaa cctcataagt ccaagtttca
     1561 tatattgaat aatcacttat caaatcgttt agtataaatc atatatcttt atcaatataa
     1621 tctttatcta ataatacttt cctatcattc attattgctt tttcgcttct acattctata
     1681 tttactattc aaaactggtt tcgtactggc tctattttat aaacatatcc cctaaatatc
     1741 cttgtatctg tattttcaac ctcataaatc tctactatat tgtattcttc tatatctatc
     1801 aaaggcatag aaaacctagc gacagcaaat gtccctatat cttcttcaat aacaacctca
     1861 ctatctatta tctctgtagt gagtactgta tctatgaact cgtgttctaa tactttgatt
     1921 aaaagtttca tacaatatat ctaataaata aaggtgtctc tatatgttat atacacaata
     1981 ggagtttcat ttagattatc tgttaatact gctatatggt ttaatcaagg gtctaggaat
     2041 atatcagctc aactattcct ttgtgctttg atatttactc cgttatctgt tacaataaaa
     2101 ttttctaatg gattattatt caagttccta ttatctaata caagatttgt tgtagttcag
     2161 tttattctat atttatttcc gttcgctgta ttgattatct ttggattggt ggctgttcaa
     2221 actatctgta tcttacaagg tgcaatccaa tttcaagcat tcgttactgg tatagctcaa
     2281 gcaaaacttc aaaaagttgt tggtagtgtt gtgcttaatg ttcaaccacc tataaatgct
     2341 tttgtacctg tgattgtatg agtagtcgcc ccatatatct tttcgctttc acttaataac
     2401 tcaaaactaa aatctattac tggactatct aatcaatttt cagcactagg cattgaatag
     2461 actttacaat atgctaccct ttcatttcac ccgtcatctt gaaactgtaa tggataaaat
     2521 catctattta ctatgtttgg atttcattca ggtttcaaag cattcaataa aagacttcgt
     2581 gctgtatgtc tttttgcttt cgttgtccct attacctttc attcaaaact aaataatcta
     2641 ggtcaagcta atgtttcact agcatatcat caatgaaaat ttgctttgtc tgttagattg
     2701 gttcttaata ctgtgttcct tacattattt tggattatat aggaatatca attacttctt
     2761 tgtgaagcaa cctgcactga gtcagtgaaa tctagtcatt tatatcttcg tgcttttcct
     2821 atcattattt gcttagttta ttaactaaat aatctacaag tgatggagat gttactgtcg
     2881 catttacagt agttgtcttt tggttgttat tcactacagt tcttgtagaa cttcaaccac
     2941 tacccatagc agaacttttt gctcttgcta gtgcattata ttttgatata agcccatcta
     3001 ttgcactttt ttgttcctct atcatacctt tgaattgttc ggtataatcc ctttccatct
     3061 gttcttttgc ttgtgtgtat gcctcaaata atgcttcctc ttcgcttagt ttgttttgta
     3121 aatctaaaag ctcttgttca gctatcgctt tttttgcttc ataatcagct ttgattttac
     3181 ctatctctgt caatgcgtca tactcttttt gtgcatcaat ctttaattgt agagcttcaa
     3241 tttcttcttc tgtcaatcaa acaaaagctt ctgccctttc agcctcaagt ttcgcccttt
     3301 ttgctgggtc atcttctcaa gcaagttctg tatctatctt tgataccctt tcagctatcg
     3361 atatgtctct atctttttct aggttcttta attctctacc tatttctcat attctttttg
     3421 cataatcttc aactcttgtt ttactctctt ctagtttttt atcataatct tctatatttt
     3481 ttattttatt agaagttgat ttatcatcta atgatgttat tttatctaat aatcaagttt
     3541 gtagttctat ttcttttttt atgctatctt ctgctaattt taattgttgt tttttattat
     3601 aatcatattc atcagttatt ttctgtattc attcagttaa actcttaaat catctctttt
     3661 ctactagatt taattcttta gtatctatta ttgataattc ttttaatcat aattgacttg
     3721 ctaatttatt atatgttttt ataattcaat tcacaaaatc agttactcag tttctagcct
     3781 tctgaaatca attaactatt caagctgcta aattttgtgc agctgtatct atattataaa
     3841 atatattttt tataaattga aaagatactt ttgtatttaa aacaaagttt tctaaaagtt
     3901 ttgttattca ctcaaataca ggaactcgca ccttattaaa tcaagctcat atactagcag
     3961 aaacatctct ccatttattt tttgatattt gtaatcttca atctgtacta gctaatcttt
     4021 tattcacctc ttcttgtaga gcattattgt ttttatattc ttcagatgct aattttattg
     4081 cttctgctaa tgttttaaag tttcaagaac ctgctaatat tgtttttctt aatcttaatt
     4141 cagttattca tagttcatct aatatacgac cagcatcagt tcaagcagaa cttattcact
     4201 ccaacacctt tacaaaagct tctcaagtat tattttgtac taatcattta aactcttctg
     4261 ctgtcattca agctactgta gcaaattttt gtaattctgc tcatcatctt gcagcagcat
     4321 catttatttg tcaaaagaat tttactatag atgttcctca agccgcagca tttattcaag
     4381 aggcgaccaa agctgtagat attcatagta tttctgtagc agtcaaatta gacaataatc
     4441 aagctgtttg tgcttccttt gcaaaattca aaatttcttg ttcattagct gcaaaattat
     4501 tacccaatca aactaaagca cttcataaat tttctatttc tgtgattgga actcataata
     4561 catcagaaaa tctttttaat gatgtagcag cctcttcagc actcaaaaca gtggctatac
     4621 ctaatcaagc tgctacctct gtaaatttta ttaaattatc tcttcatcta actcataatt
     4681 gtcaagctac ttctgctatt caagctaact cttcaaaagt taaagaaaac tgtctagata
     4741 atcttcttaa tcatttttcc aatatatcat atcattcagc tgtatcatca aatgtttttt
     4801 gtactcatac aaaagctgtt tcaaaacttc ttgctgtttt tgttccattt acaatggctc
     4861 tacctaaagc atatatagaa gatatagcta tagtcatagg attaacaaga ctctttaata
     4921 ttcaaccacc caaactatta aactttgctt gtaatctact gactgtagta tctcctgtat
     4981 tcttataatt tgtcatctgt ctagctgctt ctgttgtagc ttttgagatt ttaccaaaag
     5041 ctaactctgc ttgtgcgagt tgtttgttgt ctactattca agcatcatag agttttctta
     5101 tattctttag ttctgtttgt gcatctcttt tttttgccaa taatctacct cgttcatctt
     5161 caagtttttt tactttattc gggtctaaag cctttcaagt gtcattagca aatttctgta
     5221 tctctctttg agcttgtttt atctcctttg tgtttacttt ttttgcttgt gtgtatgcgt
     5281 ctaaagcctt tcaagtgtca ttagcaaatt tctgtatctc tctttgagct tgttttatct
     5341 cctttgtgtt tactttgaca tctattttaa taggttccat tatcaagaga tagtagataa
     5401 aatttgcttg attttctgat tttcttcttc gcttctattc tcggctcttt gtttcactag
     5461 gaataattta ttttttcttt gtcattcttt gcttgtttcg ttagcattcc aaactaatca
     5521 ctctgtaatc cattctaact gctcaaaagt atagttctcc attaaagcca aagggtgtaa
     5581 tcaagtcttt tcacttagta ttgttatata actactgaat ggtggatttt ctcccttagt
     5641 ttcgtttttc ttatcaaatc ataagtagtg tttcttaatt aaatccaata tctcccccca
     5701 tttcaccatt atacattgtt tatccctctt tgttatcttt ttatcactat gttttgtgat
     5761 aaaatccaat aaccatataa caatgttaaa catattccct tttatagttt ccccaaaatc
     5821 tatcgcttcc attactgtcg cttgtttgaa ttttaatttt ataaccttat catatttcca
     5881 aaataacaat cttttttcta tgattagact tatttcaata gattttctta ttgttctttg
     5941 cataatattg gttatatttt aaataaagca ggcaggtcac cccacctgct tatgtattta
     6001 attaagctcc ttgatagtta atctcatctt taacaagtcg tagccctcat ctattaccca
     6061 taaattcaaa cggactattt ggaacatctc aagctcttac tacatctaca aacccttgga
     6121 tcatatcact attcaatcaa gcatcgtata agtagatagt cttgttttta ccattagcat
     6181 cattgtttca tacaatcttt actactactt ttggaagctc ttttacttgt agattagaac
     6241 cataagcagt gtaatcagtt gtaccactta catctactga actaatacct aatacttttt
     6301 ctaatacatc catctcaccg atttcgtatc ggtttcaaga tatttttgca tttggagtat
     6361 atgcttgata aattactcag ttatcatctg tttcaatctt gatttggttg tcagaagtat
     6421 ttactgcgaa ctgtacatca gttacagaag ccatcctttc ataagaaaga gcatctaatt
     6481 cagatattac agttgctaca ttacttaaac tgtcatcaag tgctgtaatt gttgcccagt
     6541 ttgctacata tacttgtcaa gctagaactg ataatgcctt ttgatttgtt gctgtcattt
     6601 tttaattgtt tataatttaa aaagtttttt gtatgcgttt attggtagtt caaccactca
     6661 aggtcaatat gctactcaat ctattatagt cagtcttttc aaagttactt gctttgtttt
     6721 ttctgactta atctcttttg ttatgaattt tgttttctct atcttcatag atttgttctt
     6781 gtaatttttt tgcatttttt attctcaaga aataaaatca tctaatttat aaactggaac
     6841 acaaagtctt gaataagtag gtttctattt ttatcatcaa caaacattct atatgttcca
     6901 ccctctctaa tcttataaac ctcaaaatct cataatttat agacttgaaa gctgtttgta
     6961 gtgactaaat tatcaataag caatttagag attgcgacca gttccttttt tctcacattc
     7021 tcattatgtc atatcaatct aaactctact aatgcttgtt tatctactgc atttataacc
     7081 tgtgatactg tgtttatagt cagatatatt caggtctgct ctctcatagg ttctcaataa
     7141 taaatcctat cactcaaaac agtggctata cttgttgtag ctttcagata atcacatatt
     7201 tggtctattt ctacgacttt tgccattatt gattaagttt tctgataaat ttatttattt
     7261 caccctctat aatactttta acctcttttt cgttctcaaa ctgtgctttt gaatacatcc
     7321 tcgcacctac tcagctataa aaagggcttc aaccattcct tctaccacct catttatagt
     7381 aattatattt cttaccactt accccatatt ctacatacgg agcatagggt gttttattta
     7441 tcagtcatca agagattata tcgccttgct gtcttattgg ttgctttttg tttccctctt
     7501 gtagttcata agtatcctct ggagtgtttt tctctgcttc tttgagtaaa agctctactc
     7561 atttttctat tcaagtttta atataagttt ccaacatctc tttcacttac ttttaaaacg
     7621 atattatcta tttttcagtt tatgtcttgg tatgggaaca cactatctat aacatagctt
     7681 caaaaatcca tcatatcagt agataaatct attgtcatcc ccctacgaac taatgtcttg
     7741 tctcattgta ataccacttc taatgtttcg ttttcaaact ctctagcttg attattactt
     7801 ctatatctgt tacttgggac atagaaatca caagcaatag actgataaac aggaaaatag
     7861 cttttagtat cctctcaatc tatcgtcact atatctacat catatataga gcatatctta
     7921 tctaaaaaca ttatactgtg atggttgaat aattaaaaat agtggatttt actgtatcaa
     7981 aatcttttat atccttgaag gtcacacttc taggtcataa attatatgat tttatttctt
     8041 gtcattgtgt tttcgctaat tctcaagata ccatcaaata ttgcatatac ttaatatcat
     8101 tcggtatagt ctgatatcaa gctacatatt tgatagtgaa atatggaaaa tctagtcaag
     8161 acatatactc aactaggttt tttatatata atctactgtt tttagggtct tgtatcctat
     8221 aatcagtatc caatacccct gtatatgctt ttcagtttat ctctgtgata ctggcaacct
     8281 caagattaga taattggatt atagaatatc cgaacttata atcaacttcg cataccttta
     8341 tatcctcttg tttgtttcaa tatgataaat ctcatatcaa tcaatctaat atgtctttgg
     8401 tgctatcaat caatagacta acctgtgtgc ttgtttgtgc atcaagcgat gttactcaag
     8461 caaactgcat atactctgca ttagttatat atcccatagt ttatgagttt agtttttcta
     8521 aaataacttt ttcactccat ttaggtcaag gtcttttacc aaacttctct atatatagtt
     8581 taatgagtga ttttttgtca agagttgttt caggtttaga tgtttcaata atatctgtgt
     8641 ttggttttga tacttccaca tctcctttaa cttctctaaa atacatattt ttaatctcta
     8701 attcactttc aaagatttct cattcaacta cttttacttt atcactattt ttgtgagttt
     8761 ttaccattgt atgctctttt caagtgtatt gatatttttt taattcagcc ataatgaatt
     8821 gttaattata aagattgggg gattttatgg caaccccccg aagccattat ttaactagga
     8881 tatatttatt cataatacta ctgctgggtc tgtttcacct gcttttttgt tagcgatagc
     8941 aaaacctgca tccatagtac caacgaattg atatccttta cctggtattt tatacacagt
     9001 aatatctaat ccatttttac cgtaacctcg ttgtactgct ggtctccata catatagagc
     9061 agaaccttta gtgttgtttg atgctgttgc acttgctttt ccgtcagctt ctgttgaagg
     9121 gaaatctctg tgaacaaata aatcaactcc tgctatatta gataatgcac ctgttatagc
     9181 tgttgaattt actccattct tttggaattc taggaattca gagatagtta atgctttgtt
     9241 gtatgcttta taatccataa tcaatactaa atcttgtaga ttagtagaat atttattcat
     9301 aagccctctc aaaacaaata agtcttccat tgctagagta cctaaatctt tgtagtctac
     9361 atttacagtt cctgctagag ctgtttttct caaaccagtc catcaaagaa gtctgtggtc
     9421 tgatgctcaa ccagttgccg caaatgttgt agctggagct tggtcatcag agtttacatt
     9481 tccaacactc gaagtttcag ggtctgcatt tacgatagca ctttcaaatg ttctaacaaa
     9541 tgattgagac atttttctca aaatcattgt ttgtaaatct gcaacactgt aagccaattc
     9601 ttcgtttgat acatcaacag aaactatcat tgaatattga ttgattgtta catcaccagt
     9661 aggcaataat tgttttcctt gtgcgattgc acctgctcca gtagtccatt cagaattacc
     9721 ttgtgcaaat gggatttctc ctacgattgg tactttctct gatttaccca tagagtttcc
     9781 gtggaaacct accataaacg cattcaatat tcctgcataa gtaggaatca tttctaaaac
     9841 tgtgtcagtc aatacattta ctgggataag ttctttacca tatcctgtat ttgttgtatg
     9901 aactacttcg tttgctttcg ctctcatatc agatattttc ttttctgata attcttttac
     9961 ttcgtctgta atctctatag atttcaactc tgctaattgc tctaataatt tatccatttt
    10021 tttaaatata aatatataaa aggttttatg acttaatttt agcaatagtt tttgctaatc
    10081 aagttcataa cattacatct tcctcaggct ttttctcctc tacataaggc attgcgctgt
    10141 caatataatg gtttttgaat gctgtgtaaa gctcttttac tttacttaga catcattcta
    10201 ctactacact catatcctct ttgaatttct ttgtttcaga tttcaaatca gataaatctt
    10261 tttgaagcaa ctctatttga cttttggttt caatagatag cccctttgtt tcctctattg
    10321 tttcaagaga tttttctatt tgattgaacc ttttttccaa atcttcgttg atttcaacct
    10381 caacatcatt ctcaggtgtt tcagtatctt caacttcgga tttgtcgcta tctcaaacga
    10441 cattttcagg tatttcaggc tcttcaatag tttctttgtc ttcttcaatg ttttgttcgc
    10501 tttcagtttc ttctgtaatc tctgatattt cttcaatact tacatcttca tcagtttttt
    10561 cttcaatttc tagtgatttt tcttcaacaa cttctttttc tgtttcaatt tcatctgttt
    10621 tttcttcttc gcctacatta tcctcatttt ctttttcaac ctcttttatt tcttcttctg
    10681 tatctgtatc aactgggttt tcttcctctt tttcttctgt atctgtgttt tcttcttcaa
    10741 cttctgtaat ttcttctgta tctgtgtttt cttcttcaac ttctgtaatt tcttctttga
    10801 tttcctcttt ttcttcaatt attttctctt ttccctctac tacctcttct ttttctacca
    10861 aatcttcgct tttaatcctc aaatcttcta ttgatttgta taatgtatac tcatcattct
    10921 tagatatttg gaagttttta gcatattctt catcattcaa tcatttttct aataactctt
    10981 ttttgctttt tatctttgct tttgggttca ttggtacact taccaaacta acctcaaaca
    11041 attctagtca gtttatctgt cggtatactc aatcaggtgt atctattgtt tcatattcag
    11101 ttattctata tccaaatgac atagttttga ttacccctgt cctcaaatct tggaatatat
    11161 tatccttatc agtctttact atacctttaa catacaatca aacatcatca atagtagctt
    11221 caattatggt tcctatcgct ttgtttgagt cgtgttgtaa aaagattatt gggttctcca
    11281 taaactgttc aatagagttc ctaaatgctt ctggcattat cacatctcta cccctatcta
    11341 aatcttttgt gcttgcgtat cactcgaatt gtaatcagtt ataagtattt cagtcagcac
    11401 ccttataggt tacttctgtg atacttggtt tgttgtcctc ttgtttcaaa gacatcttga
    11461 aaaatgattg tagttttttc atattatatt atttaaaagt taaattggtt attaagattt
    11521 tctaaaaacg acatattact tcatattaga ggtttatcag ctccctctat ctcgaacttt
    11581 tttaaatccc tttcgcccct agcttcattg attgttatta ttcacaactg cacatctttt
    11641 ctttgcgact cctctatttc tttacggtct gtaaatagtt cgctatctac ttttatccta
    11701 taatcagctt ttgggtttac aaactgttta taaaagttgt ttatatcatc ctcaaactgc
    11761 atagatagat tgtttattgt ttcacttgcc tcttgtctta tcgctgtgat actcctgtca
    11821 gcacctcact ccatcataaa tcagataatt ctagggtcta tctgaaacac cattcaccat
    11881 ttctttacta taaagtttct taaatttatt aaatccaaat ctttattact tagttctagt
    11941 atcttcacat cttggatacc acctgctatc attattttat gagcattctc agaaccttta
    12001 tatttgttgt ttacatcttc tttgatttga cttagtttat ctttgtccat agtaatatta
    12061 gggtctagca taaacaaagc attaggaaca ctattatttt caaagaaata gaactgtctt
    12121 tttgagcttt cagtatcact caaagcgtca tatactatcc cttgatatat agattttcag
    12181 tagttaggat tgtttgagtc atatcttaca atactattat atagtcaatc ttttggtatg
    12241 tttctcatat tcataccact gatttgttta tatcatacta tatttcagaa tttatccacc
    12301 tcttttatta tcgttctact atccactatc tgacaagcta tatcgtttga taatgttttt
    12361 cttggataga ggtaaatatc tccactacaa aaatgattcg tgaaatattt atcttttagt
    12421 gtgcttcgtg tattatcttt gaatacatca taaacctttt tcaactcctc ttcattatct
    12481 aatacctctc aatccttttg taagtataat caatttttac ctatccagtt tactatttta
    12541 cttttatatg cttctgcttc tagtgagtag ttttgtattc tataataagt atttttatca
    12601 ataacagttt ttcaacaaga cgcaaaatcc tgtcctatca cttgaaatca aaccgagttt
    12661 atagattttt tcgagcttgt ctttgctttt ttatctttca tatacaaaaa aagataataa
    12721 taaaattatc tttactataa tatgtttttt taaaaaataa atagatattt gttatttact
    12781 ggattttaaa caattttttg agtttgaata aaattttgaa tatatttttt tgccctattt
    12841 ttcatactat aatatcacca ttcagtattt atatataata tttccataat ctcttctcta
    12901 tccttatttt ctaaataata tagtcttaca aatcaagcaa gtcttctgtt tcattttaat
    12961 tttagttctt ttattatatc ttcatacatc tttatatggt gtcaaaataa aatcaaactc
    13021 aagacaatcc gaagctatga accatactat caactgcatc atctttgttt ccactctcac
    13081 cagtaaaata tagtagttgc tctattacat tatcagtttt tcatacctta aaaaatatct
    13141 ctcctctttg gaaacaaccc tcaaactctt ttagtctagt ccgtttatct tttgttgcat
    13201 tgattgtagt aattggtaga ttatagcctc acatattcgc agggtcttta aatagtttag
    13261 cataaatctc tcaaccatta ttcttttcaa cttttatatt ccttggctta tagttaagat
    13321 acatattcaa gactgttcta catatatttt ggttattttt ctcttcgcct ttcaaactta
    13381 tctcttctag tacatatcta tataatacat tttctacttt cttaaatcca gtcactgtaa
    13441 tagaaaaaga gtcggatttt gtcttttcac tgaatgcagg gtctatacca gcctcaatat
    13501 aatcaaactc tatattttca tcaaacatcc ttatatattc tgatttgatt agtctatctt
    13561 tatttgagaa tggttttaat agatagtttt ggttaaaaga tatttctcat aaatcattta
    13621 gctttttttc taatgaaatc ttatgcttct ttggatcttc tatatcctta ttatactcct
    13681 cagcttcttt gtttgttttt gtgtatctat cccatactgg ctctccattt tcatctacta
    13741 ccttttgtga ttgtatgctc caggaagcat tgtttttata tttctgtttt agacgaggat
    13801 tgagtcaatc ttccattatc acattaccta gtatccttat ctgacaataa tcagctagac
    13861 ctcaaaacac ttcatcctct aaaaatctca tattttggtc tatgatagct ttattcttta
    13921 cgctgttgat attatcaata tcatcaagaa gtaatgagtc tggtcttacc ataccatctt
    13981 ta
//
LOCUS       DAOPCH010000016        13833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_4449_length_13833_cov_5.009798, whole genome shotgun sequence.
ACCESSION   DAOPCH010000016 DAOPCH010000000
VERSION     DAOPCH010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 13833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13833
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4449_length_13833_cov_5.009798"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..830
                     /locus_tag="PLU55_01905"
     CDS             87..830
                     /locus_tag="PLU55_01905"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HPJ86847.1"
                     /translation="MVEKKRKNNNKVILGIIILIVLGFFIYSYLTKGFFYSIVNLDQE
                     SLVNTLSSLGAFSFLAYIFLIVIGIIFAPIHPFIFYVTGGIVFGPYLSWGLTMVGVAI
                     GSSIAFKLSQKYGREFVDKHVSKKHTEKFDKISEKYGSIAIFLLRLNPLTSSDVWSYI
                     AGLTKIKFWRFLGYTLLGLAPAIFVQVYFGKEIGDNPVLFKIFVGIAILYLTVLILGL
                     TYFYFIKRKDKLSKKKENKDNREIRKKKE"
     gene            869..1618
                     /locus_tag="PLU55_01910"
     CDS             869..1618
                     /locus_tag="PLU55_01910"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HPJ86848.1"
                     /translation="MKKKSRFEIFLKKNWLILLIILIVSGLFIYSYYSRGIIYSLSNS
                     DSQSVVDFVNSFGAFSYFVFVVIVVLEVVLAPIPALALYIAGGALFGAFFGGILTIIG
                     NVIGAFIAFWLARRLGRKFVERKVDVNLRKKFDKFSQKYGVFSLFLLRVNPLTSSDLF
                     SYLSGLTNMKARTFLIGTTLGLAPMIFAHTYFGETFVNSYPILYTILLWVSVAYILVF
                     FYLIWLSTRKNKSNKGKVKNSKNKSKKKLSP"
     gene            complement(1596..3539)
                     /locus_tag="PLU55_01915"
     CDS             complement(1596..3539)
                     /locus_tag="PLU55_01915"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HPJ86849.1"
                     /translation="MTWNLYEKGKFLEPLKFSNGKTQEDVVKEVIQAVKEGNKIIFIH
                     GKCGTGKSAIALNIARELGKSSIVVPSKALQQQYKRDYEENKYILNSKNKKLKISVIT
                     GRNNHKCKFLEDNKNAIPEQKKEVNSKLYDIFEGRREKLQELISKDVSADNKNIPCKI
                     ELKEKNFNRLKEYLKQNKKINIKNFEKVSDIKRMSVAPVCPYWSPVVPEEYDVKNLDE
                     KEKIKYRGLKNKNFYFYKRKPGCGFYEQFNSYVDSDIIVFNSLKYKLESALNRKPCTE
                     VEIIDECDEFLDSFSNQRNINIDRLQNSISHARVSEEESFQIIREINEILNHLKRNNK
                     VNELIRNRGIIELKKTGLYDLFKIFIRNSEFAEEIDEESYVHDVVSTIRMFDEFLDET
                     YVLFDKREDNLIASVVTINLAKKFQEMVNKNKVVVLMSGTLHSSDVLSEIFGIDKFKI
                     IEAETENPGKINILRTGEEMDCKYSNFFSGKAERKKYLQTLDKCIEIAEKPVLIHVNS
                     FGDLPDRFEREKFDLRYVITREDLIELQIRDKENNMVKKFKEGRMNILFSTKCSRGVD
                     FPGDECKTIVFTKFPNPNVQDAFWKILNKTNPQKYWSFYKDKARREILQKIYRGVRFK
                     EDEVTLMSPDLRVLNFFKEKASS"
     gene            complement(3579..4583)
                     /gene="twy1"
                     /locus_tag="PLU55_01920"
     CDS             complement(3579..4583)
                     /gene="twy1"
                     /locus_tag="PLU55_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-demethylwyosine synthase TYW1"
                     /protein_id="HPJ86850.1"
                     /translation="MLDKNIKQLLKKQHYEIAGKNEHSAVQICRWTKKSLRDEGVCYK
                     EKFYGIKSHLCCQMTPSLYCPNKCVHCWRAIEYTTGEKIPGKIDSPSEIIDSCILSQR
                     KLLQGFKIDKNSKKIQLSKANQKKYKEAQEPMQFAISLSGEPTTYPYIGELIAELRKR
                     GKTSFLVTNGLYPEKIKELLGKNQLPTQIYISINSSNENQYVKFHRSIKKDAWKKLNE
                     TLDILPKLNGKTRTVFRMNLVKELNMFPEQAKEYAQLIKKSQPRFIELKSYMAVGNSR
                     KRLGYERMPTEKDMKNFIDVLLKELQGTGYKLLGEHSFSRAYVLGKDKKELKIKDSEL
                     "
     gene            complement(4627..6063)
                     /locus_tag="PLU55_01925"
     CDS             complement(4627..6063)
                     /locus_tag="PLU55_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPJ86851.1"
                     /translation="MNYFTAIDLTLFGIFVIFISIFLYRKRKNLKKEGLLLLYKTSWG
                     MKVIDKVSKKYKKTLGKLSYVVIGIGYLLMGFMIYLLGKIIYIYVAYPSIVKAIKIPP
                     ITPLVPYLPQAFKLNFLPDFYFIYWIIILAIIAITHEFMHGIFMRRYGIKIKSTGFGF
                     FPFFFPIFLAAFVEQDEKSMNKSKNFHQMAVLAAGTFANLITGILFFIIMILFFSWTF
                     APAGAEFNTYSYSIVSIPAIVSVNNVTIDNTNYNKIFSLIDEEGLNEIRTENNTYLIN
                     KKGLEEQRNSKTNYLLLYDDSPAIKSGINGTIVAVNGIPIRDWNEFGYELMKYSPGET
                     ISITTKSGEEKINYEIVLGENPEDSEKPWIGVGYIEQTRRGPIGKVIETISSFKKQYI
                     HYEPKFKAATFIYNLLWWLVMISISVALMNMLPMGIFDGGRFFYLTILSITKNKKKAE
                     LWFKISTNILLFMFVLLMVFWVLSFFVA"
     gene            complement(6111..6275)
                     /gene="rpl40e"
                     /locus_tag="PLU55_01930"
     CDS             complement(6111..6275)
                     /gene="rpl40e"
                     /locus_tag="PLU55_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="contains a zinc-finger motif; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HPJ86852.1"
                     /translation="MAKIPEAQNRLFKNVFVCKNCKVKMKADIQKILKGKVKCRKCGK
                     SAFRPIKKGK"
     gene            complement(6320..6649)
                     /locus_tag="PLU55_01935"
     CDS             complement(6320..6649)
                     /locus_tag="PLU55_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86853.1"
                     /translation="MKKTIERKFHPRFERILQFVVNYEDFQKLAIGDCLGILAQGRGN
                     EFSAYFEGREVYKRHQIILLQQEENLNDGPIIDKKTSKVVEYKLPLSGKINIKDFYKG
                     KVKEYPV"
     gene            complement(6710..7102)
                     /gene="eif5A"
                     /locus_tag="PLU55_01940"
     CDS             complement(6710..7102)
                     /gene="eif5A"
                     /locus_tag="PLU55_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HPJ86854.1"
                     /translation="MVLKIIDATEAKVGTNIIVDGMPCTVKSIDISKTGKHGHAKCRI
                     EAVGIITGTKKVFVVPGHERLEVPSVEKRKAQILSKSDGKASLMDLENFETIEVPCPD
                     PAVYEEIQENGNCEYWDIEGERLIKRKL"
     gene            7153..7806
                     /locus_tag="PLU55_01945"
     CDS             7153..7806
                     /locus_tag="PLU55_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPJ86855.1"
                     /translation="MALQKNDFIEIEFTGKIKDGEVFDSNIPEELKKLNPNKKAKPFV
                     FPLGQDMFLKGIEQYLMGKEVGSYEIELSPKDAFGFRDPALIQMIPMRVFIEHKINPV
                     PGAVLTFDNRFGKVLTVSGGRVMIDFNGPLAGKDVVYTVKVSRKVEDINEKIKALIEF
                     LFRRELKYEIQDKKVILNVDKGFKNFAELFKDKFKEILDLELEVHELESPNKEKEDK"
     gene            7920..9023
                     /gene="ftsZ"
                     /locus_tag="PLU55_01950"
     CDS             7920..9023
                     /gene="ftsZ"
                     /locus_tag="PLU55_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPJ86856.1"
                     /translation="MANSYEEGESFDSLSSNIQEIDRELEELLKKQSAKIKIMGIGGG
                     GNNSLSRMREIGIKGGELIAINTDAQDLLYANADQKILIGKELTQGLGAGSNPQVGQE
                     AAKESESEIKKRITGSDMIFITCGLGGGTGTGAAPVVAGIAKKQGALTIAIVTLPFTI
                     EGKKRIENAMNGLEKLEALVDTLIVIPNDKLLELAPDLPLQTAFKIADEVLTNAVKGV
                     TELVTKTGLVNLDFADVKAVMVNGGVSLIGMGEADSHNRALEAVEKAINNPLLDVDVS
                     NATGALVNVIGGPNMSLDEYKTIIGAVGDKLSPDAKLIGGAQIADDMDKSIRVLLIVT
                     GVKSSQILGSTDTLDNIRRKEIEEELGIEFFEN"
     gene            9013..9261
                     /locus_tag="PLU55_01955"
     CDS             9013..9261
                     /locus_tag="PLU55_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86857.1"
                     /translation="MKIKMSIEMDVTKMIINEKLKEEESFSLDELLKDISSQGGSNYV
                     AEDLTVKQYLDTLKEVGLLAYNPSTNRYAVLKNPNSKN"
     gene            9301..9471
                     /locus_tag="PLU55_01960"
     CDS             9301..9471
                     /locus_tag="PLU55_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase SEC61 complex subunit gamma"
                     /protein_id="HPJ86858.1"
                     /translation="MLGKTKSFLLKCKRVWQVLKKPTKKEFQLTAKVSAIGIGILGLL
                     GFLISLVMNFFK"
     gene            9541..9864
                     /locus_tag="PLU55_01965"
     CDS             9541..9864
                     /locus_tag="PLU55_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86859.1"
                     /translation="MNQHLDYGKIYRLILSDEGYNGMYLGEKVSKKRLKTNHIILVDN
                     SDFINRYKKKLDRNFLLIKFEKYWFDTNNKLILENMNYIKNLSKGEISYLEELVKKIP
                     KQNNN"
     gene            9948..10403
                     /locus_tag="PLU55_01970"
     CDS             9948..10403
                     /locus_tag="PLU55_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HPJ86860.1"
                     /translation="MIFIIKVTTNKEERAVDMISDRAKKKNLNVYSVLRPHGLRGYIF
                     LESEDRDSAEEAVFNLPYVKGIIGKTIEYSEIQGMVQPTIESVSIKEGDIVEMIGSTL
                     KGEKAKVLRIDKQKEEVVVSLFGAVVPLPVTVKIDNVKVIRREDLEDDN"
     gene            10393..11079
                     /locus_tag="PLU55_01975"
     CDS             10393..11079
                     /locus_tag="PLU55_01975"
                     /inference="COORDINATES: protein motif:HMM:NF002232.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPJ86861.1"
                     /translation="MIIKILADGGAMKPGPALSQKLGPAGIPINQVIQKINEATSDFQ
                     GLKVPVELDVNVKTKTFDVKVFSPPVSGLLKKELGIAKGSGTQLKNIVGNASIEQIIS
                     IAKTKMQNLLCKDLKSAVKVVAGTCVSLGILIENKPAKEIEKDIEAGKYDKEISQVKT
                     ETSPEKKKMLNDYLKEVQKKQDLMLKQEQAAKEAAEQAAPAATATPAATASSSKAAPA
                     TKAKEPAKKK"
     gene            11129..11202
                     /locus_tag="PLU55_01980"
     tRNA            11129..11202
                     /locus_tag="PLU55_01980"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11164..11166,aa:Pro,seq:tgg)
     gene            11225..11440
                     /locus_tag="PLU55_01985"
     CDS             11225..11440
                     /locus_tag="PLU55_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010885965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37ae"
                     /protein_id="HPJ86862.1"
                     /translation="MTTKSKKTKSAGRFGARYGKRVRDRLVQVESKQRKKQRCPFCKK
                     LGVKRLASGIWNCTKCNKKFAGDSYTV"
     gene            11451..11588
                     /locus_tag="PLU55_01990"
     CDS             11451..11588
                     /locus_tag="PLU55_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010478759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit P"
                     /protein_id="HPJ86863.1"
                     /translation="MATYKCFKCGKKVTDKNLEKRFLCPFCGSKIFFKPRTKVKVVKA
                     E"
     gene            11616..11930
                     /locus_tag="PLU55_01995"
     CDS             11616..11930
                     /locus_tag="PLU55_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016272 - prefoldin complex [Evidence
                     IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit"
                     /protein_id="HPJ86864.1"
                     /translation="MKEKNIQEIQFLEQGLQNILFQKQAFQMELSEAKEALTELDNSK
                     DEVFKIIGQIMIKSDKKKIKDEISSKIKLFELRIGELEKQESSLTSRIEKIRDENLKK
                     SK"
     gene            12031..12420
                     /gene="sepF"
                     /locus_tag="PLU55_02000"
     CDS             12031..12420
                     /gene="sepF"
                     /locus_tag="PLU55_02000"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HPJ86865.1"
                     /translation="MVFKKIKKAFSMSGGRDGDEYIEIDLTKEEKDNKVMVKLFTLKQ
                     YEDINEVLNALREGYTITVVEIKTLKQKDPIELKRAISKLKKTTDALEGTIAGFGENL
                     VIATPSFAKIQKVEKPKPEPKDKYEDY"
     gene            complement(12532..12918)
                     /locus_tag="PLU55_02005"
     CDS             complement(12532..12918)
                     /locus_tag="PLU55_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86866.1"
                     /translation="MKSHENFVGAWLFLIGVILAVLIGIATAFFIPISKIATFSTPIY
                     LILVILGIIVGIKINVSGKDSQNFLISSAVLVIVSRFGMESVRGSLIGIGMGDLVSST
                     FSALLVLFVPATIIVAIKSLFSIANV"
     gene            13103..>13833
                     /locus_tag="PLU55_02010"
     CDS             13103..>13833
                     /locus_tag="PLU55_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86867.1"
                     /translation="MKTKTFNLLFASVFALVFLIGFSSATISLTSQVTTLSQSSGSFD
                     FTVSSDQNETIDLSIPSISDGSGHTIIFSLSSEQITIDTSVEDSVLITVDYVVESGFN
                     FQFEKTYTAKLNATGSVSGEVTKAFNFEGSSFCEFSNQGNLKVTVKDIKVTSDSFGKD
                     NKWFVFDTVEVKVEIRNSGREDIEDVVLEWGLYNTQSKKWTIEVDEEDEFDIDKGDRE
                     SITIKFKLDDSMDEDLEDLSKGKYI"
BASE COUNT         4937 a         1968 c         2070 g         4858 t
ORIGIN      
        1 agcaagtgag cagggccctt tgggcaaccc ggttcgactc cgggcggggg cgtttcattt
       61 ttaaacatca tttcatatgg agttatatgg tggaaaaaaa gaggaagaat aacaacaagg
      121 ttattcttgg aataattatc ttaattgtat tgggtttttt catctattct tatttaacaa
      181 aaggattttt ctattcaatt gtaaatcttg accaggagag tttagttaat actttaagtt
      241 ctttaggagc attttccttc ttagcttata tttttttaat tgtcataggg attatttttg
      301 ctccaattca cccattcata ttctatgtta caggaggaat agtatttgga ccctatcttt
      361 cttggggatt aactatggtt ggggttgcaa ttggctcttc aattgcattt aaactctccc
      421 agaaatatgg aagagaattt gttgataaac acgtttcaaa aaaacacaca gaaaaatttg
      481 ataaaatttc agaaaaatat ggttcaatag caattttcct tttaagatta aatcctctaa
      541 catcctcaga tgtctggagc tacatagcag gattaacaaa aataaaattc tggagatttt
      601 taggatatac tcttttaggt ttagcaccag caatatttgt tcaggtttat tttggtaaag
      661 aaataggaga caaccctgtt ttattcaaaa tatttgtagg aatagcaata ttatatttga
      721 cagtcttaat tttaggttta acctatttct attttataaa aagaaaagat aagctctcta
      781 aaaagaaaga gaataaagat aatagagaga ttagaaagaa aaaagaataa ttaagtatat
      841 ttatataata tattgagttt attatcaaat gaaaaaaaag agtagatttg aaatttttct
      901 taagaaaaat tggttaatcc ttttgataat tttaattgtc tcaggattat ttatttattc
      961 ttattattca agaggaataa tctactctct atctaacagc gattcacaat ctgtcgtgga
     1021 ttttgtaaat tcttttggag cattttctta ttttgtattt gtggttatag ttgttttaga
     1081 agtagtgctt gcaccaatac ctgctcttgc attatacatt gcagggggag cattgtttgg
     1141 agcttttttt ggagggatat tgactattat aggcaatgta ataggagcgt ttattgcatt
     1201 ttggttagca agaagattgg gaagaaaatt tgttgaaagg aaagtagatg ttaatttaag
     1261 aaaaaaattt gataaattct ctcagaaata tggagtattt tcactttttt tactaagagt
     1321 aaatccccta acatcttcag atttgtttag ttatctttca ggtttgacaa acatgaaagc
     1381 taggacattt ttaattggaa caactcttgg actagctcct atgatatttg cacatactta
     1441 ttttggtgaa acttttgtga atagttatcc tatcttgtat accattcttc tttgggtaag
     1501 tgttgcttat atcttggtat tcttttattt aatttggttg tcaacaagga aaaataaatc
     1561 taataaaggc aaggttaaaa attctaaaaa taaatctaag aagaagcttt ctccttaaag
     1621 aaattcaata ctcttaaatc aggactcata agtgtaactt cgtcctcttt aaacctaacc
     1681 cccctataaa ttttttgaag tatttctctt cttgctttat ctttgtagaa actccaatac
     1741 ttttgtggat tagttttatt taatatcttc cagaatgcat cttgtacatt aggatttgga
     1801 aactttgtaa atacaattgt cttgcattca tctccaggaa aatcaactcc tctagaacac
     1861 tttgttgaaa aaagaatatt cattcttcct tctttgaact ttttcaccat attattttct
     1921 ttgtctctga tttgcaattc aattaaatct tccctagtta ttacatatct taagtcaaat
     1981 ttttcccttt caaatctatc tggtaaatct ccaaaactat ttacatgaat taatacaggt
     2041 ttctctgcaa tttcaataca tttatccaat gtttgaagat atttttttct ttctgcttta
     2101 ccagaaaaaa aattagagta tttacaatcc atttcttcac ctgttcttaa gatatttatt
     2161 tttccaggat tttcagtttc agcttctata attttgaatt tatcaattcc aaatatttct
     2221 gaaagaacat cgctggaatg aagtgttcca gacataagaa caaccacttt gtttttattt
     2281 accatttcct gaaatttttt tgcaagattt attgttacaa cactggcaat aagattgtct
     2341 tctcttttat caaataatac ataggtctcg tccaaaaatt catcaaacat tcttatcgta
     2401 ctaacaacat catgaacata gctttcttca tctatctctt ctgcaaattc agaatttctg
     2461 ataaatattt taaacaaatc atacaatcct gtttttttta attcaattat tcctctattt
     2521 cttattaatt catttacttt attatttctt tttaggtggt ttaatatttc atttatttct
     2581 cttattattt gaaaactttc ttcttcactc actcttgcat gggaaataga attttgcaat
     2641 ctgtcgatat taatatttct ctgatttgaa aaactatcta aaaattcatc gcattcatct
     2701 attatttcaa cttctgtgca tggctttctg ttaagagcac tttctaattt gtatttgagg
     2761 gagttaaata caattatatc tgaatcaaca taagaattga attgttcata aaaaccgcat
     2821 ccaggttttc ttttgtaaaa atagaaattt ttatttttaa gtcctctgta ttttattttt
     2881 tctttttcat ccaaattttt tacatcatat tcttcaggaa caaccggact ccaataagga
     2941 caaacaggag caactgacat tctttttata tcagacactt tctcaaaatt ttttatattt
     3001 atttttttat tttgttttaa atattctttt aatctattga aattcttttc tttaagttct
     3061 attttgcatg gaatattttt gttatctgca gaaacatctt tagaaattaa ttcctgtaat
     3121 ttttctcttc tcccttcaaa tatatcatat agttttgaat tgacttcttt tttttgttct
     3181 ggtattgcat ttttattgtc ttctaaaaat ttacacttgt gattatttct tccggttatt
     3241 acagatattt ttaatttctt gtttttacta ttaagaatat atttgttctc ttcataatct
     3301 ctcttgtatt gttgctgtaa tgctttactc ggaacaacaa tagacgattt tccaagttct
     3361 cttgcaatat ttaacgctat tgcactttta cctgtaccgc atttaccgtg gataaatatt
     3421 atcttatttc cctctttaac tgcctgtata acttctttta caacatcttc ctgcgtttta
     3481 ccattcgaga attttaatgg ttcaagaaat ttcccctttt catataaatt ccatgtcata
     3541 agttatgaga aatttaagtg caattataaa atttgctgtt ataattcgct atcttttatc
     3601 tttaattctt ttttatcttt gcctagaaca tatgctcgtg aaaatgaatg ttctcccaat
     3661 aatttatatc ctgttccttg aagttcttta agtaaaacat caatgaaatt tttcatatct
     3721 ttttctgttg gcattctttc ataacctaat ctttttcttg aattcccaac agccatataa
     3781 ctttttaatt caatgaatcg aggttgtgat ttttttatta attgagcata ttcttttgcc
     3841 tgttctggaa acatattcag ttccttgact aaattcattc taaaaacagt tctggttttt
     3901 ccatttaatt ttggaagaat atctagagtt tcatttaatt tcttccaagc atcttttttt
     3961 atgcttctat ggaattttac atattgattt tcatttgaag aattaattga gatataaatt
     4021 tgagttggaa gttgattttt tcctaaaagt tcttttattt tttctggata aagtccattt
     4081 gtaacaagaa aagatgtttt acctcttttt cttaattctg ctattaattc tccaatataa
     4141 ggataagtag ttggttctcc acttaaacta attgcaaatt gcattggttc ttgagcttct
     4201 ttgtattttt tttgatttgc tttacttaat tgaattttct ttgaattttt atctatctta
     4261 aacccctgaa gaagttttct ctgagagagt atgcatgaat ctattatttc agatggagaa
     4321 tctatttttc caggtatttt ttcccctgtg gtatattcaa ttgctctcca acaatgcaca
     4381 catttatttg gacaataaag cgagggagtc atctgacaac ataaatgaga ctttattcca
     4441 taaaattttt ccttataaca aactccttca tcacgcaaag atttttttgt ccatctacaa
     4501 atctgaacag cagagtgttc attctttcca gctatttcat agtgctgttt ctttaagagt
     4561 tgttttatat ttttatcaag cataattaaa ttaactatat cttgtttaaa agagtaatta
     4621 tttagattag gctacaaaaa aactcaatac ccagaatacc attaaaagaa caaacatgaa
     4681 aagcaatata tttgtagata tcttgaacca taattcagct ttctttttgt tttttgtaat
     4741 acttaatatt gtaagataaa agaatcttcc gccatcaaat attcccattg gaagcatatt
     4801 cattaaagca acacttatac ttatcataac aagccaccaa agtaaattat aaataaatgt
     4861 agctgcctta aatttaggtt cataatgtat gtattgtttc ttaaaagaag atattgtttc
     4921 aataactttt cctattggtc ctcttctggt ttgttcaata tatccaaccc caatccaagg
     4981 tttttcagaa tcttctggat tttctcctaa aactatctcg taatttattt tttcctctcc
     5041 actctttgta gttatactta ttgtttctcc tggagaatat ttcataagtt catacccaaa
     5101 ttcgttccaa tctcttattg gtatcccatt taccgctact attgttccat ttattcctga
     5161 ttttattgca ggagagtcat catataaaag aaggtagttt gttttagaat ttctttgttc
     5221 ttccagacct tttttattta tgagataagt attgttttct gttctaattt catttaatcc
     5281 ttcttcatct atgagagaga atattttatt ataattagta ttatctatag ttacattatt
     5341 gactgaaact attgcaggta ttgaaactat agaatatgaa taagtattga attctgcacc
     5401 agcaggagca aacgtccaac tgaaaaacaa aatcattatt ataaagaaca atatcccagt
     5461 tattaaatta gcaaatgttc ctgcagctaa tactgccatt tgatgaaagt tcttgctctt
     5521 attcatactc ttttcatctt gttcaacaaa tgctgctaag aatattggga agaaaaatgg
     5581 aaagaatcca aatcctgtag attttatttt tattccatat cttctcataa atataccatg
     5641 catgaattca tgagttatag caattatagc aagaattatt atccaataaa taaaatagaa
     5701 atcaggaaga aaatttaatt taaatgcttg gggcaaataa ggaacaagag gtgttattgg
     5761 aggtattttt atagctttaa caattgaagg ataagcaaca taaatataaa ttatttttcc
     5821 aagtaaataa atcataaatc ccatgagaag ataaccaata cctataacaa catagcttaa
     5881 ttttccaaga gtttttttgt attttttact taccttgtct attaccttca taccccaact
     5941 ggttttatat aataaaagca atccttcttt tttcagattt tttctttttc tgtaaagaaa
     6001 aatagatata aaaataacaa atataccaaa caatgtcaaa tcaatagcag taaaataatt
     6061 catcttctcc tctcagttta aagtaaaatt aggtttaaaa gtgtatggtt ttacttgcct
     6121 ttctttatag gtctaaaagc actctttcca cattttctac acttaacttt tcctttaagg
     6181 attttttgaa tatctgcttt catttttacc ttgcaattct tacaaacaaa aacattcttg
     6241 aacaatctat tttgtgcttc aggtatcttt gccatattaa aacattgaaa aattagtttt
     6301 taaatcttgt gagggtttat taaacaggat attctttaac tttgccttta tagaaatctt
     6361 taatgtttat ttttccagat aatgggagtt tatattccac aactttagaa gtttttttat
     6421 ctattattgg accatcgttt aaattttctt cttgttgtaa caaaattatc tggtgtcttt
     6481 tatatacttc tcttccttca aaatatgctg aaaactcatt tcctctacct tgagcaagaa
     6541 tacctaaaca atctccaata gctagttttt ggaaatcttc ataatttaca acaaactgta
     6601 aaatcctttc aaatctagga tgaaattttc tttcaattgt tttcttcatt gaaacaaaaa
     6661 actattttaa tttttaaata attatattcc caaaaataaa tttcaaagat tatagttttc
     6721 ttttgattaa tctttcacct tcaatatccc aatattcaca atttccattc tcttgaatct
     6781 cttcataaac tgcagggtct ggacaaggaa cttctattgt ttcaaaattt tctaaatcca
     6841 ttaaactagc tttaccatct gatttagaga gaatctgggc ctttcttttt tcaacacttg
     6901 gaacttccaa tctttcatgt cccggcacaa caaaaacttt ttttgttcca gttattattc
     6961 caacagcttc tattctacat ttagcatgcc catgtttgcc tgttttagat atgtcaatac
     7021 tcttcactgt acatggcatt ccatcaacaa taatatttgt acctactttt gcttcagttg
     7081 catcaataat ttttaacacc ataatctaat caaaaaaaat acatttataa acgtttttaa
     7141 ttaacagact tcatggcact tcagaaaaat gattttattg aaattgaatt tacaggaaaa
     7201 atcaaagatg gagaggtgtt tgattctaat attccagaag aattaaagaa gttgaatcca
     7261 aataaaaagg caaaaccatt tgtatttcca ctaggacaag atatgttctt gaaaggaatt
     7321 gaacaatatt taatgggaaa agaagttgga agttatgaaa ttgaactctc tccaaaagat
     7381 gcttttggtt ttagggaccc tgccctgata caaatgattc caatgagagt tttcatagaa
     7441 cataaaataa atccagttcc aggtgcagtt ttaacttttg ataataggtt tgggaaagta
     7501 ttaaccgttt ctggaggcag agttatgatt gacttcaatg ggcctctagc aggaaaagat
     7561 gttgtttata ctgtaaaggt ttcaagaaaa gtagaagata taaatgagaa aattaaagct
     7621 ttaattgaat ttttattcag gagagaactt aaatacgaaa tccaagacaa aaaggtaatt
     7681 ttaaatgtag ataaaggatt caagaatttt gcagaattat ttaaggataa attcaaagaa
     7741 atacttgatt tagaattaga agttcatgaa ttagaatccc caaacaaaga gaaagaagat
     7801 aaataattat atttttataa tattttatct atattaaatc atcctgcaat cagaaagaag
     7861 agatatttca gtaaaagata acaacaatac ttaaaaagca aaataacgtt tctttatgta
     7921 tggcaaactc atatgaagaa ggtgaatctt ttgactctct ttcctcaaat atccaagaga
     7981 tagatagaga attggaagaa cttttgaaaa agcaatcagc taaaataaaa ataatgggta
     8041 ttggaggcgg aggaaacaat tccttgagta gaatgaggga aattggaata aaaggaggag
     8101 aattaatagc aataaatact gatgctcaag atttacttta tgctaatgca gaccagaaaa
     8161 ttcttatagg taaagagttg actcaaggct taggtgctgg aagcaatcct caagtaggac
     8221 aagaagcagc taaagaatct gaatctgaaa tcaaaaagag aattacaggc agcgatatga
     8281 tattcataac ttgtggtctt ggaggaggga caggaacagg tgcagctcca gttgtagcag
     8341 ggatagcaaa aaaacaagga gcacttacaa tagcaattgt tacattacca tttacaattg
     8401 aaggtaaaaa aagaattgaa aatgcaatga atggtttaga gaaattagaa gcactagtag
     8461 acacattaat agttatccct aatgataaac ttcttgagct tgctccagat ttgcctctcc
     8521 aaactgcatt taagatagca gatgaagttt tgacaaatgc agttaaaggc gtaacagaat
     8581 tagtcacaaa aaccggtctt gttaatttgg attttgcaga tgttaaagct gttatggtta
     8641 atggaggagt ttctttaata ggaatgggag aagcagacag tcataatcgc gctttagagg
     8701 ctgttgaaaa agcaataaat aatccccttt tagatgttga tgtttcaaac gctacaggag
     8761 ctctagttaa tgttattgga ggtccaaata tgagtcttga cgaatataaa acaataattg
     8821 gagcagttgg agataaatta agtccagatg caaagttaat tggaggagca cagattgcag
     8881 atgacatgga taaatcaata agggtattat taattgttac aggagttaaa tcctctcaga
     8941 tattaggaag cacagacact cttgataaca tcagaagaaa agaaattgaa gaagaattgg
     9001 ggatagaatt ttttgaaaat taaaatgtca attgagatgg atgtaaccaa aatgattatt
     9061 aatgaaaagc ttaaagaaga ggaaagcttt tcattagacg aacttttaaa agatataagt
     9121 tctcagggag gttcaaacta cgtagcagaa gatttgactg ttaaacaata tctggacact
     9181 cttaaagaag taggcttgtt ggcttataat ccttcaacta acagatacgc tgttcttaaa
     9241 aatccaaata gcaaaaacta aattgaataa ttttaaaaac cctttacatc ttttaataat
     9301 atgttaggaa aaacaaaatc atttttattg aagtgcaaaa gagtttggca agtcttaaaa
     9361 aaaccaacca aaaaagaatt tcagctcaca gctaaagttt ctgcaatagg tattggaata
     9421 ttagggcttt taggtttttt aatatcttta gtaatgaatt tctttaaatg attgattatt
     9481 tatgctctta tagtccaaaa ccacataaat ttttattaat cattattctt aaattaaatt
     9541 atgaatcagc atttagatta tggaaaaata tacagattaa tcctttctga tgaaggatat
     9601 aatgggatgt atcttggaga aaaagtatct aaaaagagac taaaaacaaa tcacataata
     9661 ttagttgata attctgattt tattaaccga tataagaaaa agcttgatag aaatttttta
     9721 ttgattaaat ttgaaaagta ttggtttgat actaacaaca aattaattct ggaaaatatg
     9781 aattatataa agaatctatc taaaggagaa attagttatc ttgaagaatt agtaaagaag
     9841 attccaaaac aaaacaataa ctaaaacttt ataaatacac aattttttaa actagcatct
     9901 gcttcttaga tattaagagg tagttggaac ataactagga aaacaaaatg attttcataa
     9961 taaaagtaac aacaaataaa gaagaacgtg ccgtcgatat gatttcagac agagcaaaaa
    10021 agaaaaatct taatgtatat tctgtgctaa gacctcatgg attaagagga tatatttttc
    10081 ttgaatcaga agacagagat tctgcagaag aagcagtctt taatttgcct tatgtgaaag
    10141 ggataatcgg aaaaacaatt gaatattctg aaatacaagg aatggttcaa cctacaattg
    10201 agagcgtttc tataaaagaa ggagacattg ttgaaatgat aggttcaaca cttaagggtg
    10261 aaaaggcgaa agttttaaga atagacaaac aaaaagaaga agtagttgtt agcttgtttg
    10321 gagcagttgt tccattacct gttacagtta aaatagataa tgttaaagtt atcagacgtg
    10381 aggatttaga agatgataat taaaatctta gcagatggag gggcaatgaa gcccggtcct
    10441 gctttaagcc agaaattagg tccagcagga atcccaataa atcaagttat ccaaaaaata
    10501 aatgaagcaa cttctgattt tcagggatta aaagttcctg tagaattaga tgtaaatgtt
    10561 aaaacaaaaa cttttgatgt caaggttttc tctcctcctg tatcaggact tcttaagaaa
    10621 gaattaggaa tagccaaagg ttcagggacc caacttaaga atatcgttgg aaatgcttct
    10681 attgaacaaa taatttctat agcaaaaaca aaaatgcaga atttattatg taaggattta
    10741 aagtctgctg ttaaagttgt tgcaggcact tgtgtaagtt taggaatatt aattgaaaat
    10801 aaaccagcta aagaaattga aaaagatatt gaagcaggta aatatgacaa agaaatctct
    10861 caagttaaaa cagaaacttc tcctgaaaag aaaaaaatgt taaacgatta cttaaaggaa
    10921 gttcaaaaga aacaggattt aatgcttaaa caggaacaag cagctaaaga agcagcagaa
    10981 caagctgccc ctgcagcaac tgcaactcct gcagcaactg catcctcttc aaaagcagct
    11041 ccagcaacaa aagctaaaga gcctgcaaag aaaaagtaaa caagctaatc aaatacttta
    11101 taaattatca ttagctattt tttcataggg ggttatgggg taacctggtt agccttcgag
    11161 gtttgggact tcgcgatccc ggttcaaatc cgggtagccc cacttaaaca aaagacaata
    11221 agaaatgaca acaaaatcca agaaaacaaa atcagcagga agattcggag caagatatgg
    11281 aaaaagagtt agagatagat tagttcaagt tgaatctaaa caaagaaaaa aacaaagatg
    11341 ccctttttgt aaaaagctag gagttaaaag attagctagt ggaatttgga attgcactaa
    11401 atgtaataaa aaatttgcag gagatagtta tactgtttaa ataatttaaa atggcaacat
    11461 ataaatgttt taaatgtgga aaaaaggtta cagacaaaaa ccttgaaaaa agatttcttt
    11521 gccctttttg tggaagcaag atttttttca aaccaagaac aaaagtgaaa gtagttaagg
    11581 cagagtaatt tttcattaaa ttcacaacaa ataaaatgaa agaaaaaaac atacaagaaa
    11641 tacaattttt agaacaaggt ttacaaaata ttttgtttca aaaacaagca tttcagatgg
    11701 aattatctga ggcaaaagag gctttaacag aattagataa ttccaaagac gaggtattta
    11761 aaataatagg acaaataatg attaagtcag ataaaaaaaa gattaaagac gagatttctt
    11821 ctaaaataaa gttatttgaa ttaagaatag gtgaacttga aaagcaggaa tcctctctta
    11881 catccagaat agaaaaaata agagacgaaa atttaaagaa atctaagtga aatctgttgt
    11941 ttttgtcttt ttgttattgt tattatataa agataaaaat agctaaaaaa aatccgaaat
    12001 ctttataaat ctgtcaatta taaatttatc atggtgttta aaaaaataaa aaaagctttt
    12061 tcaatgtctg gtggaagaga tggagacgaa tatattgaaa tcgatttaac caaggaagaa
    12121 aaggataata aagtaatggt taagcttttc actctaaagc aatatgaaga tatcaatgaa
    12181 gtcctcaatg ctttaaggga aggttataca attactgtag ttgaaataaa aactttaaag
    12241 caaaaagacc ctattgagtt gaagagagcg atttcaaaac ttaagaagac aactgatgct
    12301 cttgagggaa caattgcagg atttggagag aatttggtta tagcaacacc ttctttcgca
    12361 aagatacaga aggtagaaaa accaaaacca gaaccaaaag ataaatacga agattactaa
    12421 tttttctttt tgacatagaa aattgaaatt ctgtgggaat gatgttttaa ttgaaattag
    12481 ttaatattct cctattgtct attatctatt aatatgtaga acttctttgt tttatacatt
    12541 tgctatagaa aataaggatt ttatagctac aattatagta gcaggaacaa aaagcacaag
    12601 taaagctgag aaagttgaag aaactaaatc tcccatacct atccctatta atgaacccct
    12661 tacactttcc atcccaaatc tgcttacaat aactaatact gcactagata ttaagaaatt
    12721 ttgagaatct ttacctgaaa cattaatctt tattcctact attataccaa gaattacaag
    12781 tatgagataa ataggggtgc taaaagtagc tattttagat attggtataa agaatgcagt
    12841 tgcaattcca ataagcacag ccaaaataac tcctataaga aataaccaag ctcctacaaa
    12901 attttcatga gatttcatta ccattttaat ttacttgttt gattaaatac cttagtttac
    12961 aaattatata aactttttga aatatgttgt atcgacgtaa atttaactaa atttaatttt
    13021 ttggtcaata tgctccaaaa aaagatatat cttgaaaaat agaaatgttt ataaaccata
    13081 gtaactccag aatgattaca aaatgaaaac gaaaacattc aaccttttgt ttgcaagcgt
    13141 ttttgcatta gtattcctaa taggattttc aagtgcaaca atttctttaa cttcacaagt
    13201 tactactctt tcacaatcaa gtggttcttt tgatttcact gtttcaagtg atcagaatga
    13261 aactattgat ttatctatac cttctatttc agatggttca ggacatacaa ttatattttc
    13321 attatcatct gagcagatta caatagatac aagtgttgaa gattctgttc ttataacagt
    13381 tgattatgtt gttgaatctg gatttaattt tcaatttgaa aagacctata cagctaaatt
    13441 aaatgcaact ggaagtgttt caggtgaagt aacaaaagca tttaattttg aaggttcaag
    13501 tttttgtgaa ttttcaaatc aaggaaattt aaaggtaact gtaaaggata taaaagtcac
    13561 atcagacagc tttggaaaag acaataaatg gtttgtattt gatacagtag aagttaaagt
    13621 tgaaataaga aattcaggac gtgaggatat agaagatgta gttttggaat ggggattata
    13681 taatactcaa tcaaaaaagt ggacaataga agttgatgaa gaagatgaat ttgatataga
    13741 taagggagat agagaatcaa ttacaataaa attcaaatta gatgattcta tggatgaaga
    13801 tttagaagat ttaagcaaag gaaagtatat ttt
//
LOCUS       DAOPCH010000017        13668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_4501_length_13668_cov_4.388673, whole genome shotgun sequence.
ACCESSION   DAOPCH010000017 DAOPCH010000000
VERSION     DAOPCH010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 13668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13668
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4501_length_13668_cov_4.388673"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..656)
                     /gene="pcm"
                     /locus_tag="PLU55_02015"
     CDS             complement(48..656)
                     /gene="pcm"
                     /locus_tag="PLU55_02015"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HPJ86868.1"
                     /translation="MDREGLIKSLRAKGFKDNIIQAFCNVKREDFVNEGFQRLAYEDI
                     ALPLTNDQTISQPSTIALVLSLLKLRENQKVLEIGSGCGYTLALITEIIGEQGKAYGA
                     EIVKELADLSVEKLKKYSNVKIYNKNGKEKFEEAPFNRILISAALKEIPHEILDQLAD
                     NGIIVAPLGSGFLQTLTAIRKISGKYIIEKEIPGFMFVKFID"
     gene            complement(720..3578)
                     /locus_tag="PLU55_02020"
     CDS             complement(720..3578)
                     /locus_tag="PLU55_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007076 - mitotic chromosome condensation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HPJ86869.1"
                     /translation="MAYIKRLVMHGFKSFPRKTEIPFTSGINVILGPNGSGKSNISDA
                     LCFVLGRLSIKSMRAAKARNLIFLGTKAASPAKEAIVEIVFDNTDKTFSIESEEISIK
                     RIVRLNGQSIYKINNETKTRQEVLGLLAQAGIDPNGFNIILQGEIQNFVRMHTEERRK
                     IIEEVSGISVYESRKEKSLKELEKTEEKQKEVFSILRARTAYLNNLEKERQQALKYKK
                     LEGDIKKFKASIIKVDINKRKSHLKEIEESIAKKNKEIEKIKQEISKLSEGIKNYEEK
                     IVAINKKIQESTGLEQEKLNQEITSIRADIAGLNVKIENSENKINSILKQKLENQESI
                     RKEELELKELQKESPSVVKKEKEIVSKKLELEKLEERRKRFYMIKSELKSIREKFEDK
                     KSALQGYDNESNFLVKQIESLSGQLFDKRTSPESVEELKSSLYNKKSLLENLNRRQIE
                     LEKISFSSEKEIEKEKKITETISKMDICPLCKSKITKEHIENIHKEIYPKISSLTKEL
                     EDSDKELSQIGMKKEILSQEIEKISSEVSKREMDLIKISNINEKKNAIKSLNDKIIIS
                     RKELAEIEKLKNNLEKELEENSNVEQKYETARIELQEISLRTKENVNSEIAFKQREYE
                     RLKISLKQLSREEEDVKEELTGFKKKFDEKEKILEKKKKQEEELIQKFQAMIKERDDL
                     QAKIRKDDQDTLIKKNFIYNIEQEINNVKIEKAKIDAEIENLELDMLNFPDIETIRGS
                     REFLTEKLAKIQEAFSQIGPVNMRSVEVYDEIKKEYDVINEKANKITKEKESILKIIH
                     DIDVKKKKVFLKTLGELNEIFSRNFAQISVKGQVSLELENPKEPFEGGVGILVKTGHG
                     KYFDVTSLSGGEQTMVALSLIFAIQELNPYCFYILDEIDAALDKRNSERLGGLLRKYM
                     QKGQYIVITHNDEVITNATNLYGISMHEGISKVVSLKV"
     gene            complement(3696..4367)
                     /gene="rnhB"
                     /locus_tag="PLU55_02025"
     CDS             complement(3696..4367)
                     /gene="rnhB"
                     /locus_tag="PLU55_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPJ86870.1"
                     /translation="MGDFILGIDEAGKGPVIGPMVIAGCLIDTSIEREFKRLGIKDSK
                     ELTPKRREFLANIVREKAETFEIIVVHPKEIDKSLNSGMNLNNLESRKIAEIINKINK
                     GYKKIKVVIDCPSVGVTKWVDALKMKINNLSNLEITCEHKADKNYVAVSAASILAKSI
                     REKEMNILREKYGDEIGSGYTSDPATIHFLELHADNPKNASIFRKTWETWKKASEKLT
                     QKKLI"
     gene            complement(4442..4897)
                     /locus_tag="PLU55_02030"
     CDS             complement(4442..4897)
                     /locus_tag="PLU55_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86871.1"
                     /translation="MEKVLEKALKTKVEEIEQNLELDFVKGPAYPVSVKIKEQGFDIA
                     GHGKVRTKYPLSSFIGILEDKEPIKKSFFGIPYSKHSKAEYLGTLWFNNSKKGANEYS
                     KWVLNVNGRENLSKMTKLGKMLSKEYKIPVKLSLGTEYAVEENYILDLL"
     gene            4998..5073
                     /locus_tag="PLU55_02035"
     tRNA            4998..5073
                     /locus_tag="PLU55_02035"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5033..5035,aa:Met,seq:cat)
     gene            5323..5850
                     /locus_tag="PLU55_02040"
     CDS             5323..5850
                     /locus_tag="PLU55_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86872.1"
                     /translation="MGRGVKIYFAFLFLILIAGIVCAYPSFSLVNEDTNSKKAVIVYN
                     VDPIEVINDYSTVIQLNYLYGDFADLIEYLRNTKIMTILYYNGDINSETVKYLEEESG
                     VNFRRFLKNVNKAQKLNSLNEEEILNTPEIIISGKSIEQQNSVKFTFVTSLTIIIIIS
                     MIVVVKSMSKEKKKR"
     gene            6034..6804
                     /locus_tag="PLU55_02045"
     CDS             6034..6804
                     /locus_tag="PLU55_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPJ86873.1"
                     /translation="MKRKMPLIALAQIKYNHTGRHNVDRIKKYIVLAAEKKADIVCFP
                     ETCINKAQKLPLNHKLIKEIQETCKEHSIWAIITDNFISKGTHYKMAILINREGKIKG
                     KYKKIHLYGDGVEENGKRIFVRDTDFGKIGIAICWDLAFPKLFRKMKKAGAEIVFCPS
                     YWGYEAKAHKEEPKLNELNLLKSMVLSRAFENLNFVCVCNPYKKEWDLIDYSAIASPH
                     KILTELYDKEGLVMSRINLNEIEKFTKLYPGKKPGEEK"
     gene            complement(6807..7370)
                     /locus_tag="PLU55_02050"
     CDS             complement(6807..7370)
                     /locus_tag="PLU55_02050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPJ86874.1"
                     /translation="MNKTLKLLIISDIFVLSGFGLISPILAIFIKDNLVGGTITAAGV
                     ASTIFMITHALLQLLFAYKFNPKDRLWMLRLGTALFVLVPIGYIFSTHIYHIYIIQFV
                     YGIAAAFAYPSWSSLFTSNLEKGKRGFQYSIYSSSVSIGTAITAGVGALLAERIGFEL
                     VFATTGLFAIIGFFILMKLEKKILKKI"
     gene            7492..7572
                     /locus_tag="PLU55_02055"
     tRNA            7492..7572
                     /locus_tag="PLU55_02055"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7524..7526,aa:Ser,seq:gct)
     gene            7607..8068
                     /locus_tag="PLU55_02060"
     CDS             7607..8068
                     /locus_tag="PLU55_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86875.1"
                     /translation="MSVKKRRFNIWITLSILFIIFIVLILIIRLTEKNYTTDDISSEC
                     LSIEITNVVDKGENNYEVELITKEKIKNKNFGGVALVFTNSTSILNDMKYVDTYYISN
                     REYSLIKLMNEVTIPVSISNLYNVSTIYVIPYFFDDSGNIIHCLFANQYVI"
     gene            8115..8273
                     /locus_tag="PLU55_02065"
     CDS             8115..8273
                     /locus_tag="PLU55_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86876.1"
                     /translation="MAMVDLEDIHSIERAKKKNSVSRKHIYCQKCGRAKRCHDPECTQ
                     LHCNCGQL"
     gene            8296..8580
                     /locus_tag="PLU55_02070"
     CDS             8296..8580
                     /locus_tag="PLU55_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86877.1"
                     /translation="MDKIDITDIEEDDFEDEEELDDEIEDEEYEDESEEEKIENERYE
                     QYANLIKQKTDRELIESISVRLFSLETDIKHIRDDLVDLYNLVLENQEID"
     gene            complement(8606..9976)
                     /locus_tag="PLU55_02075"
     CDS             complement(8606..9976)
                     /locus_tag="PLU55_02075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPJ86878.1"
                     /translation="MEEERLLKEQGKKYSLKDGASSSVMEGMGLKYITPYALSLGLSN
                     KLIGILEVLPTLIANILRIFLSKAYYKKSRKNMVLPFIFIQAFFWIPLLLVGVAYFFF
                     QLKLIYASLFLIICYSIIIISGLIASPAWTSWMQDLVETNRGEFFSKRSKINGFVIAI
                     SMITASIILDSFKGEKVFLGFILLFSLAAIGRYFSFHFLKKQYEPQPTRDDKAFFSFL
                     QFAKKMHSNNFGRFVIFTSLMSFAVVIASPFFSVYMLKELQLSYFAFTLIVLSSLVSP
                     IFFLSFIGKKIDKYGTVKVMKISGFLISFVPLLWIASLLLMNSPQWVLIGYLFAVEFF
                     SGFVWAAYNLSVSNFIYDAVTKPKIILCFTYFGFVNSIGSFLGGLIGGQLSGSASFSI
                     FGLGAILSVFLLSTVLRILPSIFILPKLKEVRQVETSKAIIQLKVKEKLEYFIKLITS
                     YNPRPT"
     gene            complement(10049..10873)
                     /locus_tag="PLU55_02080"
     CDS             complement(10049..10873)
                     /locus_tag="PLU55_02080"
                     /inference="COORDINATES: protein motif:HMM:NF016312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPJ86879.1"
                     /translation="MFQKYLVLASKKDKAGMNITNHLSQFRENPLLSSWGNKPSFDLC
                     FVEEETIYTNNLDLEKINQYDLIIFASKHQSEKKEKSLSVHAPGNFRTADFGGEPEKV
                     CPASALFNKLLFTKLQENAENASLDYKVTLECTHHGPLISKPCVFIEIGSTETEWVDR
                     RAGFILAKTIKETIEEFQENKYREIAVGLGGPHYCPGFNKLQLKSNVAFAHIIPEYVM
                     PISETFILETIKKTVEPVDFVVLDWKGLGKSEQRQAVIDILEKNYIQWKKTSDITR"
     gene            complement(10928..11233)
                     /locus_tag="PLU55_02085"
     CDS             complement(10928..11233)
                     /locus_tag="PLU55_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86880.1"
                     /translation="MVDRRYEGFSPKDKAVEYFLEIVSDCRELHPTAALDKFDDLEYL
                     FRQEGFNGDLIGKLNKAKNLLDSLESYNTHSKEYFEQFKEFKNHLAKVQEEFCDKYR"
     gene            11325..11579
                     /locus_tag="PLU55_02090"
     CDS             11325..11579
                     /locus_tag="PLU55_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86881.1"
                     /translation="MKNKQENMVKITYKDKVERIYGILEECEAKGNAERESSIKLLQE
                     SFSLYNEVISFKGTRYCSRNLSDVPTRINNLVTKLMEYNS"
     gene            complement(11626..11811)
                     /locus_tag="PLU55_02095"
     CDS             complement(11626..11811)
                     /locus_tag="PLU55_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HPJ86882.1"
                     /translation="MALGIGGTLVGIVALICAVWIIYDVWANQKKMSSGYKILWTVAA
                     IVFSIFTAIVYYFVNRK"
     gene            11893..12534
                     /locus_tag="PLU55_02100"
     CDS             11893..12534
                     /locus_tag="PLU55_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPJ86883.1"
                     /translation="MGIISEILGQIALFCISAISSMGYLGIGFLMILESMIFPIPSEL
                     VMPFAGFLIAEGKMTFFWVVFSSIIGSLIGSLLSYYIGFKGGKKFLVKYGKYFLLNEE
                     HLAKTEKWFSKKGELTIFIGRFIPIVRHFISIPAGFGRMNLKRFILYTAIGAGIWNSF
                     LMYVGFVLGNNWESISHYSDYLSWGVLIILIILGIYFVIKRLNKKSRKYKKHR"
     gene            12546..12821
                     /locus_tag="PLU55_02105"
     CDS             12546..12821
                     /locus_tag="PLU55_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86884.1"
                     /translation="MNEVIKLALGIFVLILGIPIGNVLARNTPEELKDGRKWFYLIIL
                     VCFVGAIVSLIIRNDALLFTFLFIAIVTSRSIKKDKKEVKRRRKNKK"
     gene            12901..13665
                     /locus_tag="PLU55_02110"
     CDS             12901..13665
                     /locus_tag="PLU55_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86885.1"
                     /translation="MGKRGQVTIFIIVAVLIIAGVALFFAFRHNLSEKEETVTKDYAP
                     LYNYLQECLEQSLIEVIYINSMQGGYYDVQQDFVEFDSSSEMEGSSIPYYLIDDKIQI
                     LSKEELENQISVGLVSKFTSCLNFDEFEYNISYNQEEIFASSFITENKIRAKLYSSVY
                     INEMENTLRLKNFTAEVETNYLEYYNFAKYLTEKQRLSGEELCISCLVKECEAKNYNL
                     YLYPAVSDNSYILINTLNSKEDDLFFSRMSSRNDIA"
BASE COUNT         4563 a         1922 c         1894 g         5289 t
ORIGIN      
        1 tcttttttta ttttttattt agtctttctc catttctttt ttaaaattta atcaataaat
       61 ttgacaaaca taaatccagg tatttctttt tcaataatat attttcctga gatttttctt
      121 attgcagtta gagtctgaag aaatcctgag cccagaggag caactattat tccattatct
      181 gctaattggt caagtatttc atgaggtatc tcttttagag ctgcacttat taaaattcta
      241 ttaaatgggg cttcttcaaa tttttctttc ccatttttat tatatatttt aacattagag
      301 tattttttta gtttttcaac tgagaggtct gctaattctt ttacaatctc tgcaccatat
      361 gcttttcctt gttctcctat tatctctgtt atgagtgcca gagtgtatcc gcatccagaa
      421 cctatttcca atactttttg attttctcta agttttaaaa gagagagtac tagtgctatt
      481 gttgaaggtt gtgagattgt ttggtcattt gtaagaggca gagcaatatc ttcataagct
      541 aatctctgga atccttcatt tacaaaatcc tctcttttaa cattgcaaaa tgcttgaatt
      601 atgttatctt taaatccttt agccctcaaa ctcttaatta atccctcacg atccattaaa
      661 tatagaagca taacttttat ttaattgttt tgaaaaaatt gcatacttaa gaatataaat
      721 tagactttaa ggctcacaac tttacttata ccttcatgca tcgagatacc ataaagattt
      781 gtagcatttg tgataacttc atcattgtga gttataacaa tatattgtcc tttctgcatg
      841 tattttctta agagtcctcc taaacgttct gagtttcttt tatctagagc tgcatctatt
      901 tcatccaata tatagaaaca ataaggatta agctcttgta ttgcaaatat taatgagagt
      961 gctaccattg tttgttctcc tcctgaaaga gaagtaacat caaaatactt accatgccct
     1021 gttttaacta atattcccac tcctccttca aaaggttctt taggattttc aagttcaaga
     1081 gaaacctgtc ctttgacact tatttgtgca aaatttctag aaaatatctc atttaattct
     1141 ccgagagtct taaggaaaac tttctttttc tttacatcaa tatcatggat tatctttagg
     1201 atgctttctt tttcttttgt tatcttgttt gctttttcat ttattacatc gtattctttt
     1261 tttatttcat catagacttc aactgaacgc atatttacag gacctatctg agaaaatgct
     1321 tcctgtatct ttgctaattt ttcagttaaa aattccctag aacctctaat agtttctata
     1381 tcaggaaaat taagcatatc taattctaaa ttctctatct ctgcatctat ctttgctttc
     1441 tctattttga cattatttat ttcctgttct atattatata tgaaattctt ttttataaga
     1501 gtgtcttgat catcttttct tatttttgcc tgaaggtcat ctctttcttt tatcattgcc
     1561 tggaactttt gaattagttc ttcttcttgt ttctttttct tttcaagtat cttctctttt
     1621 tcatcaaatt tctttttaaa acctgtaagc tcttctttaa catcctcttc ttctcttgag
     1681 agttgtttca aggaaatctt taaacgttca tactctctct gtttaaaagc aatttctgaa
     1741 tttacatttt cttttgtacg caaagatatt tcctgaagtt caattcttgc agtctcgtat
     1801 ttttgttcta cattagaatt ttcttctaat tctttttcaa gattattttt taatttctct
     1861 atctcagcta attctttcct tgagataatt atcttgtcat taagactctt tattgcattt
     1921 ttcttttcat ttatatttga tatttttatt aagtccattt ctctcttaga aacctctgaa
     1981 gatatttttt ctatttcttg agataaaatt tcttttttca tccctatttg agagagttct
     2041 ttgtctgaat cttctagttc ttttgtaagt gaagatattt ttggatagat ttctttatga
     2101 atattttcta tatgttcttt tgttatcttg cttttacata aaggacaaat atccatcttg
     2161 gatattgtct cagttatttt tttctctttt tcaatctctt tttcacttga gaaagaaatt
     2221 ttttctaatt ctatttgtct cctatttaaa ttttctaaaa gagatttttt attatataaa
     2281 gaagatttca attcttcaac tgattcagga gaagttcttt tgtcaaataa ttgtcctgat
     2341 agtgattcta tttgtttgac taagaaattt gattcattgt cataaccttg gagagcagat
     2401 tttttatcct caaatttttc tcttattgat tttaattctg atttaatcat ataaaatctt
     2461 tttcttcttt cttcaagctt ttcaagttct aatttttttg atactatttc tttttctttt
     2521 ttaacaacag aaggagattc tttttgtagt tctttaagtt caagttcttc ttttcgtatt
     2581 gactcttgat tttctaattt ttgtttgaga atagaattta ttttattttc actattttct
     2641 attttaacat ttaatcctgc aatatctgct cttatagaag ttatttcttg atttaatttt
     2701 tcttgttcaa gtccagttga ttcttgtatt ttcttattta ttgctactat cttctcttcg
     2761 taatttttta taccttcgct aagcttggat atttcctgct ttattttttc tatttcctta
     2821 tttttctttg ctattgattc ttctatctct ttcagatgtg attttctttt atttatgtct
     2881 acttttatta tacttgcttt gaattttttt atatctcctt caagtttttt atatttgagt
     2941 gcttgctgcc tttctttttc aaggttattc aagtaagctg ttcttgctct taatatagag
     3001 aaaacttctt tttgtttttc ttctgttttt tcgagctctt taagggattt ttcttttctt
     3061 gattcatata ctgaaattcc tgaaacttct tctattattt ttcttctttc ttctgtatgc
     3121 attcttacaa aattttgtat ttctccctgt aggattatat taaatccatt agggtctata
     3181 cctgcctgag caagcaatcc aaggacttct tgtcttgttt ttgtttcatt atttattttg
     3241 tagatgctct gtccgtttaa tcttactatt ctttttatag aaatttcttc tgattctatt
     3301 gaaaaagttt tatctgtgtt atcaaaaaca atttctacaa ttgcttcttt tgcaggagaa
     3361 gctgctttag ttcctaaaaa aataagattt cttgctttag ctgcacgcat agattttatg
     3421 cttaatcttc ctaaaacaaa acataatgca tctgatatat tagattttcc tgaaccattt
     3481 ggtcctaata taacatttat acctgatgta aaaggtattt ctgtttttct tggaaaactc
     3541 ttgaaaccat gcattaccag tcttttaatg taagccatta aaaataacct ccaagttttt
     3601 ttctatttgc atcaccagtt ttttattctt agtcatttaa aataaccctt aaaatcagaa
     3661 gttttttggg tttttaagca ttaatataga atatcttaaa tcaatttttt ttgagtgagt
     3721 ttttctgaag cttttttcca ggtttcccaa gtttttctaa agatgcttgc attttttggg
     3781 ttatctgcgt gcaattctaa aaaatgaatt gtagctgggt cagaagtgta tcctgaacct
     3841 atttcatctc catacttttc tcttaagata ttcatttctt tttcacgtat tgattttgct
     3901 aatattgatg ctgctgaaac tgcgacatag tttttgtctg ctttgtgttc acaagttatt
     3961 tcaaggtttg ataaattatt tattttcatt tttaaagcat caacccattt agttacacca
     4021 acagaaggac aatcaataac cacttttatt tttttgtaac ccttgtttat cttatttatt
     4081 atctctgcta tttttcttga ttcaagatta tttagattca ttcctgaatt taaactctta
     4141 tcaatttctt ttggatgaac tactataatc tcaaaggttt cagctttttc tcttactata
     4201 tttgcaagaa attcccttct ttttggagtt aattctttgc tgtcttttat tcctaatctt
     4261 ttaaattctc tttcaattga agtgtcaatt aagcaaccag caataaccat tggccctata
     4321 acaggtcctt ttcctgcttc gtctatacca agaataaaat cacccataat attcttaaaa
     4381 caagtttgtt tttaaaaatt gttgttgtaa atagtgtaaa acaagaaaat atgtgataaa
     4441 cttaaagaag atctaaaata taattttctt caacagcata ttctgttcct aagcttaatt
     4501 ttacaggtat tttgtattct tttgaaagca tctttcctaa cttagtcatt tttgagagat
     4561 tttctcttcc attaacattc aaaacccatt tggaatattc gtttgctcct tttttagagt
     4621 tattgaacca taatgttcct agatattctg ccttagagtg tttagaatat ggtattccaa
     4681 aaaatgattt ttttattggt tctttgtctt ctaaaattcc aataaaactt gagagaggat
     4741 atttagtcct tacttttcca tgccctgcta tatcaaaccc ctgttctttt atttttactg
     4801 atacaggata tgctggaccc tttacaaaat ccaattctaa attttgttct atttcttcta
     4861 cttttgtctt tagtgctttt tctaatactt tttccatata attacagtct tttagatgtt
     4921 ttataaacat ttatatgagt taatcaaagc cattatattc gaagaattta aaaacgaaaa
     4981 acatctttga aatagatagc ggggtagagc agtctggagt gctcgtcggg cccataaccc
     5041 ggaggtcgtg tggttcaaat cccacccccg ctacttttga ttttaaaatg aaccattgga
     5101 taaagatact aattcaccta ttgatatttg tggatttact ttttctttct actttaagtg
     5161 atttttttgc agtgttattt gtattgtata ttgcctatct tattctttta ggggttaaat
     5221 tatctataat ccatcttgga aaaattatga gaggagagaa taaaggcaga aaaaaagcaa
     5281 aaaaccctaa aacttaaata taagttaaac cttaaataaa tgatgggaag aggggtgaaa
     5341 atatattttg ctttcttgtt tttaatttta attgcaggga ttgtttgtgc ttatcctagt
     5401 ttctctttag taaatgaaga tacaaattca aaaaaagcag ttattgttta caatgttgac
     5461 cctatagaag tcattaatga ttattcaaca gtaattcaac ttaattatct ttacggagat
     5521 tttgcagatt taatagagta tttaagaaat acaaaaataa tgactattct ttattataat
     5581 ggagatatta attctgaaac tgtaaaatat cttgaagaag aatctggagt taatttcaga
     5641 agattcttga aaaatgtgaa caaagctcaa aaattaaatt ctttaaatga agaagagata
     5701 cttaatactc ctgaaataat aattagtgga aagtcaattg aacaacagaa ttctgttaaa
     5761 ttcacttttg tgactagttt aactattata attataattt ctatgattgt ggttgtaaag
     5821 tccatgtcta aggaaaagaa aaaaagataa ggtgatgtat tagtaactac atattaaaaa
     5881 ataaaattaa tttagtaact acatatgtaa attttgatta tacgaacttt aattttcaag
     5941 aagcctttaa aaatctttgc tttatcgacg acaaatttaa ataccctgaa ggcgagttaa
     6001 aattgattta aatgaattcc tatacttttt acaatgaaaa gaaaaatgcc tctaatcgca
     6061 cttgcacaaa taaaatacaa tcatacagga agacataatg ttgacagaat aaagaaatat
     6121 attgttctag ctgctgaaaa aaaagcagat attgtgtgtt ttcctgaaac ttgtataaat
     6181 aaagctcaaa aattaccttt aaatcacaaa ttaataaaag aaattcaaga aacctgtaaa
     6241 gaacattcta tttgggcaat aataacagat aattttattt ctaaaggaac acattataaa
     6301 atggctattt taattaatag agaaggcaaa ataaaaggaa aatacaagaa gatacatctt
     6361 tatggtgatg gagttgaaga aaacggcaaa agaatttttg taagagatac tgattttggt
     6421 aagattggaa ttgctatttg ttgggacttg gcttttccaa aactattcag aaaaatgaaa
     6481 aaagcaggtg ctgaaattgt tttttgtcct tcttattggg gatatgaagc taaagctcat
     6541 aaagaagaac ctaaattaaa tgaattgaat ttattgaaat caatggtgct ttcaagagct
     6601 tttgaaaact tgaattttgt atgtgtgtgt aatccttata aaaaagaatg ggatttaatt
     6661 gattattctg ctattgcttc accacataaa attcttacag agttgtatga taaagaaggt
     6721 ttagttatgt caagaattaa tttaaatgaa atagaaaaat tcactaaact ttaccctggt
     6781 aaaaaacctg gtgaagaaaa ataaatttat attttcttta aaatcttttt ttcaagtttc
     6841 attaaaataa aaaaacctat aattgcgaat aaaccagttg ttgcaaatac taattcaaat
     6901 cctattctct cagctaacaa tgctccaact cctgctgtta ttgctgttcc tatacttaca
     6961 gaagatgaat aaattgagta ctgaaatcct ctttttcctt tttcaagatt agatgtgaat
     7021 aaacttgacc aagaaggata tgcaaatgct gctgcaatac cataaacaaa ttgaattata
     7081 taaatatggt aaatatgtgt tgagaaaata tatcctattg gaactaaaac aaacaaagca
     7141 gttcctaatc tcaacatcca taatctatct ttaggattaa atttgtaagc aaataaaagc
     7201 tgaagaagtg catgagttat catgaaaatt gtactggcta ctcctgctgc agttattgtt
     7261 cctccaacaa gattatcttt tatgaatatt gcaagaatag gagaaattaa accaaaacca
     7321 cttaaaacaa atatgtctga tattattaaa agttttaatg ttttattcat atttgtcttt
     7381 tacctcaggg taattagaaa attttgatta taaatctttt gaaataagat tattgaaaaa
     7441 ccatatattt ataaggattt taggcaaata ttttaatata ctcaaatatg agccgccttc
     7501 gcatagtggt attgcagtgg attgctaatc cacgcccttc ggggctggca ggttcgattc
     7561 ctgcaggcgg cgttgttttt ataaatatct aattattagt tcatttatga gtgttaagaa
     7621 aagaagattt aatatttgga taactttatc aatattattt attatattta ttgttcttat
     7681 tttaataatt cgtttgactg aaaaaaatta tacaacagat gacataagtt cagaatgttt
     7741 atctattgaa ataacaaatg ttgtagataa aggagaaaac aattatgaag tggaattaat
     7801 aaccaaagag aaaattaaaa ataaaaattt tggaggagta gctttagtat tcactaattc
     7861 tacaagtata ttaaatgata tgaagtatgt tgatacctac tatatatcta atagagaata
     7921 ttctttgatt aaattaatga atgaagtgac aatacctgtt agtattagca atttatataa
     7981 tgtaagtaca atttatgtta tcccatattt ttttgatgat tctggaaata ttatacattg
     8041 tctatttgcc aatcaatatg tgatttaaga caatttatga taggttttta aacaatcatt
     8101 tcgtatactt atttatggca atggttgatt tagaggacat ccattcaata gagagggcaa
     8161 agaaaaaaaa ttctgtaagt cgtaagcaca tttattgtca aaagtgtggt cgtgcaaaac
     8221 gctgtcatga tccagagtgc actcaattac attgtaattg tggacaactt taaatttcaa
     8281 agagtattgg ataacatgga taaaatagac ataacagata ttgaagaaga tgactttgaa
     8341 gatgaagaag aattagatga tgaaattgaa gatgaagaat atgaggatga gagtgaagaa
     8401 gaaaaaatag aaaatgaaag atatgaacaa tacgcaaatt taataaaaca aaagacagac
     8461 agagaattaa ttgagagtat ttctgttaga ttattttcat tagaaacaga tataaaacat
     8521 ataagagatg atttagtaga tttgtataat cttgtacttg aaaatcagga aattgactaa
     8581 ttgaattgca taaataaaat aatctttaag ttggtcttgg gttataagat gttattaatt
     8641 ttatgaaata ttctagtttt tcttttactt tcaattgtat tattgcctta gaagtttcaa
     8701 cttgtctaac ttcttttaat tttggtaata taaatattga aggtaaaatt cttaatactg
     8761 tacttaaaag aaatacagat agtattgctc ctaatccaaa tatagaaaat gatgctgaac
     8821 ctgaaagttg tcctccaatc aatcctccta aaaatgaacc tattgaatta acaaatccaa
     8881 aataagtgaa acagagtatt atctttggct ttgttactgc atcataaatg aagttagata
     8941 ctgagagatt atatgctgcc catacaaacc ctgaaaagaa ttcaactgca aaaagatacc
     9001 ctattaaaac ccattgtgga gagttcatca ataataacga agctatccat aataaaggca
     9061 caaatgaaat caagaatccg gatattttca taactttaac tgttccatac ttatcgattt
     9121 tttttcctat aaatgaaagg aaaaatatag gtgagacaag tgaacttaaa actattaaag
     9181 tgaatgcaaa ataagaaagc tgtaattctt ttaacatata tactgagaaa aacggagagg
     9241 ctattacaac agcaaaagac atcaaagatg taaatataac aaatcttcca aaattattag
     9301 aatgcatttt ttttgcaaat tgtaaaaagc tgaaaaatgc tttatcgtct cttgttggtt
     9361 gtggttcata ctgctttttt aagaaatgaa atgaaaaata tcttcctatt gcagctaaag
     9421 agaaaagtaa aataaatcct aagaaaactt tttctccttt aaaagaatca agtataatac
     9481 ttgctgtaat catgcttatt gctataacaa atccatttat cttactcctt ttactgaaaa
     9541 attctcctct atttgtttca actaagtcct gcatccatga agtccaggca ggagaggcaa
     9601 ttaaccctga gattattatt attgaataac atattatcaa aaataatgaa gcataaatta
     9661 gttttaattg aaaaaagaaa tatgcaactc caaccaaaag caggggtatc cagaaaaatg
     9721 cctgaatgaa tatgaaaggc aaaaccatat tctttctaga ttttttgtaa taagctttac
     9781 ttaaaaaaat tcttaatata tttgcaataa gagttggaag aacttctaat atacctatta
     9841 atttgtttga gagtcctaat gaaagtgcat aaggagttat gtattttaac cccatacctt
     9901 ccattacact agaagaagca ccatctttga gactatattt ctttccttgt tccttcaata
     9961 atctttcttc ttccatatat ttccatatct atccttccct cctcggttaa taagtctgag
    10021 aaatagataa attgtattat ttaaaaacct atctcgtgat atcagaagtc tttttccatt
    10081 gaatataatt cttttcaagt atatctatta cagcttgtct ttgttcactt ttacctaagc
    10141 ctttccaatc caagacaaca aaatctactg gttccactgt tttttttatt gtttcaagaa
    10201 taaatgtttc tgatattggc ataacatact ctggaattat gtgggcaaaa gcaacatttg
    10261 attttaattg aagcttatta aaaccagggc aataatgcgg tcctcctaaa cctactgcta
    10321 tttctctgta tttgttttct tgaaattctt ctattgtctc ttttattgtt tttgcaagta
    10381 tgaatcctgc tcttctgtct acccattctg tttcagttga acctatttca atgaaaacac
    10441 agggtttgga aattaatgga ccgtgatgag tgcattcaag agttaccttg taatctaaag
    10501 aggcattctc tgcattttct tgaagttttg tgaacaaaag tttattgaat aatgcagaag
    10561 ctggacagac tttttctggt tctcctccaa aatctgcagt tctaaaattt ccaggagcat
    10621 gaacagataa acttttttct tttttctctg attgatgttt tgaagcaaat attattaagt
    10681 catattgatt tattttttct aaatccaaat tattcgtata tattgtttct tcttcaacaa
    10741 aacataaatc aaaactaggt ttatttcccc aggatgataa aagaggattt tctctaaatt
    10801 gagaaagatg atttgtaata ttcatgccag ctttatcttt tttacttgct aggaccaagt
    10861 atttttgaaa catagaagta aaataaacaa accatttaaa ttatttttga ttataaaatt
    10921 caacaattta tctatattta tcacagaatt cttcttgtac ttttgcaaga tgattcttaa
    10981 attctttaaa ttgttcaaag tattccttgg aatgtgtatt ataagattct aatgaatcta
    11041 ggagattttt tgctttattt aattttccta ttaaatctcc attaaaacct tcttgtctaa
    11101 ataaatattc taaatcgtca aatttatcta gtgcagctgt tggatgaagt tctctgcaat
    11161 cagaaactat ttccaaaaaa tactccactg ctttatcttt tggactgaaa ccctcatatc
    11221 ttctatctac catattagat tttagtgaaa cactcttttt ttatgtatgt gtactaatat
    11281 aataactttt ttaaagtatt ctatacagga aaataaacaa taaaatgaaa aataaacaag
    11341 aaaatatggt taaaataaca tacaaagata aagttgaaag aatatatggc attttagaag
    11401 aatgtgaagc aaagggcaat gctgaaagag aaagttctat aaaattactt caagaatctt
    11461 tctcattgta taatgaggta atatctttta aagggactag atattgttca agaaatttat
    11521 ctgatgttcc aacgagaata aataatcttg taacaaaatt aatggaatat aattcttaaa
    11581 ctaatataaa aaataaaaag gagattaaag aaaaataaag ttctcctatt ttctattaac
    11641 aaaataataa actattgcag taaatatact aaatactatt gctgcaactg tccagagtat
    11701 tttataccca cttgacattt ttttctgatt tgcccagaca tcataaataa tccagacagc
    11761 acagattaaa gcaacaatac caactaaagt tcctccaatt cctaatgcca tattatacct
    11821 cctaaaataa ttaaataagg agtgtattta aaattatgtt aaatataaaa agtgtttttt
    11881 acttaaaaaa agatgggaat aatatcagaa atactaggac agatagcact attttgtatt
    11941 agtgcgattt cttcaatggg ttatttaggt atcggatttt taatgattct cgaaagtatg
    12001 atttttccaa ttccttcaga attagttatg ccttttgctg gttttttaat tgcagaaggt
    12061 aaaatgactt tcttttgggt tgtattttct tctataattg gaagtttaat tggttctttg
    12121 ttgtcttatt atattggatt taagggagga aagaaattcc ttgttaaata tggaaaatat
    12181 tttttattga atgaagaaca tttagctaaa acagaaaaat ggttttctaa aaaaggtgaa
    12241 ttaaccatat ttattggaag atttatacct attgtaagac atttcatttc aatacctgct
    12301 ggatttggaa gaatgaatct taaaagattt attttgtata ctgcaatagg agcaggaata
    12361 tggaactcat ttttaatgta tgttggattt gttctaggaa ataactggga gagtataagc
    12421 cattattctg attatttatc ttggggtgtt ttgataattt tgattatact tgggatttat
    12481 tttgttataa aaagattaaa caagaaatca agaaaatata aaaagcatag ataataatat
    12541 aatttatgaa tgaggtgatt aaattagctt taggaatttt tgttcttata ttggggatac
    12601 ctataggaaa tgttcttgca agaaatactc ctgaagaact taaagatggt agaaagtggt
    12661 tttatttgat tatacttgtg tgctttgttg gggcaatagt gagtttaata attagaaatg
    12721 atgctctact atttactttt ctttttattg caattgtaac ttcaaggagc ataaaaaaag
    12781 ataagaaaga agttaagaga agaaggaaaa ataaaaagtg atatattgaa gaaaatcaaa
    12841 taaaatttaa aattaaccga gtatagaaaa tattaaaaga tatattttat tgatataaag
    12901 atgggtaaaa ggggacaagt tactattttt attatagttg ctgtattaat aattgctggt
    12961 gttgctttgt tctttgcttt tcgccataat ttatctgaaa aagaagaaac tgtaacaaaa
    13021 gattatgcac ctctttataa ttatcttcag gagtgtttag aacaatcctt aatagaagtg
    13081 atttatataa atagtatgca aggaggatat tatgatgttc agcaggactt tgtagaattt
    13141 gatagttcta gtgagatgga aggctcttct attccatatt acttaattga cgataaaatt
    13201 caaatattat ctaaagaaga acttgaaaat caaatatctg ttggtttagt ctcaaaattt
    13261 acctcttgtc ttaattttga tgaatttgaa tacaacataa gttataacca agaagagatt
    13321 tttgctagct catttataac tgaaaataag ataagggcga aattatattc ttcagtttat
    13381 ataaatgaaa tggaaaatac tttacgatta aaaaatttta ccgccgaggt tgaaacaaac
    13441 tatcttgaat actataattt tgctaaatat ttaactgaaa aacaaagact atcaggagaa
    13501 gaattgtgca tttcttgttt agttaaagag tgtgaagcaa agaattataa tctttattta
    13561 tatcctgcgg tttcagataa ttcttacata ttaataaata ctttaaacag caaagaagat
    13621 gacttgtttt tttcacggat gtcttcgcga aatgatattg cataatgc
//
LOCUS       DAOPCH010000018        13421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_4601_length_13421_cov_55.907527, whole genome shotgun
            sequence.
ACCESSION   DAOPCH010000018 DAOPCH010000000
VERSION     DAOPCH010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 13421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13421
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4601_length_13421_cov_55.907527"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLU55_02115"
     CDS             complement(<1..290)
                     /locus_tag="PLU55_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HPJ86886.1"
                     /translation="MKNINIVYKKIDELKPYKNNPRKNDKAVKAVADSIKEFGFKVPI
                     ILDSNMEIIAGHTRLKAAIKLKYKEVPCIIADDLTPDQVRAFRLVDNKVGELA"
     gene            complement(265..1320)
                     /locus_tag="PLU55_02120"
     CDS             complement(265..1320)
                     /locus_tag="PLU55_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HPJ86887.1"
                     /translation="MEKRLYSNEIVFRGHPDKVCDQISSAILDACLRQDPYTRAGIEV
                     VGGKGKIFITGEITTKAKFNAKAIAKRVLSDVGYESDKYTIINNIGLQSADIALGVDN
                     GGAGDNGMMFGYACNDTEKYLPTAMVILQELAKGYDELRQNLSGLFYPDGKAQITGVY
                     DDDFNLIKIKDFTICYQNSENDRLKTDAIIKKLADNICEKHGVKVDKYLFNPTGKFLI
                     GGFDGDAGLTGRKIVVDAYQSFANVGGGCMNGKDPTKVDFSAAHKARQIAVRYLKEHN
                     LKWCEVQLSYAIGVKQPLAIYIVSDKGNIEAPESLYKECEPQEIIKDLKLREISYEEL
                     AQFGHFGGSNEKYKHCI"
     gene            complement(1516..2067)
                     /locus_tag="PLU55_02125"
     CDS             complement(1516..2067)
                     /locus_tag="PLU55_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86888.1"
                     /translation="MSIYFILRNYRARDILVQIRGEKAYFITIDKPLLTIDDDNCEYY
                     IYLRPIKSEGLIYHKKLGYIIQNNKNPIVNQNKVIENKIIGYYTIENYIKLITKEVLN
                     AFDKFNNESMTINIKHNKYLSTSMTIYKEDIPKNSEYFYLNIIKEIRLFNHPLKLDLF
                     SKLNGKPITTTNPRDMMRIKLFN"
     gene            complement(2445..>2690)
                     /locus_tag="PLU55_02130"
     CDS             complement(2445..>2690)
                     /locus_tag="PLU55_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86889.1"
                     /translation="ITMNKTHRIYTKDELQTLNDLRKKKAEVSNLIKNLYSEKAIRDR
                     RKQYAKIKKEIARIHNRAIIRYKERYGNSIIKYENNN"
     assembly_gap    2691..2790
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2907..3206)
                     /locus_tag="PLU55_02135"
     CDS             complement(2907..3206)
                     /locus_tag="PLU55_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86890.1"
                     /translation="MNKIIWVVEIRDYLDDLTLPLITFAFDSYDEAKGYVDEYIERLF
                     EKIKKPYRHSQVSEISTYDERKRQFIAVMYELYNPEIIKYVARATISKVMFYEEV"
     gene            complement(3347..3826)
                     /locus_tag="PLU55_02140"
     CDS             complement(3347..3826)
                     /locus_tag="PLU55_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86891.1"
                     /translation="MNTNTNNNNNNNNETPTLLTEHHKSLYQHLEISTNFGFEVNVEM
                     IARAFPHVYNFKSQKGNYSNCPLLYQHIDELNASNEIDKIIIKNNNKFKLGTEQEAID
                     YYNKMISKAMRLLVKASVVKRKISKHGQAKLIDNDFNYNDPLSFIETFITTNKGEKN"
     gene            complement(3828..4697)
                     /locus_tag="PLU55_02145"
     CDS             complement(3828..4697)
                     /locus_tag="PLU55_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86892.1"
                     /translation="MAKKRMFTNDILSSDAFVDLPLSAQALYIHLCMKADDDGFCDKP
                     RQIMRNIGATLGDIQLLIQKRFILTFENSSVVVIKHWKMHNTIQKDRYSPTNYQRQYA
                     LLKTNKNKSYTLVLNNNTNNKKCIHNVSKMDTDCFQNGNTDIDLDIDLDIDLDIDKNK
                     DYKDIKDINTTEVVVVSQKNNNNDKIIEAKEKRIKLEKRLTRDKTALTKELIKRKFIN
                     SDDDFAIMQLDELYYQIDLNRLFPISYKQFIIVVHNILKTYDPEKVDNKIAWFKVSLI
                     NNLEKLKERGKKK"
     gene            complement(4974..5408)
                     /locus_tag="PLU55_02150"
     CDS             complement(4974..5408)
                     /locus_tag="PLU55_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPJ86893.1"
                     /translation="MYNKIVIVGRLVRDPILRRTQNGIAVFTESVVVNDRPKQGGNYK
                     DIETTFIDVVFWDKTAENVAKYQKKGSLLFIEGVLEQRRYTNHAGEKRTTYEVIADRV
                     LFLEPKPAETQDSDENTKAPEQQPVPNSDISDKMAEYDDLLF"
     gene            complement(5470..5991)
                     /locus_tag="PLU55_02155"
     CDS             complement(5470..5991)
                     /locus_tag="PLU55_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86894.1"
                     /translation="MKLTANNIYINYLPDGKSAIGFTLDEPRLAKLAVEQYKDKAIDL
                     TISEIKSKRTQAQNRNLWQIIESISTKLYGDRTAENLNKVYCYLLKQTNIKRVLIATT
                     KEALPLIEQQFRAILLTGQTIKTINTITNKESELITLWAYSGSSSFNTKEMSELIDLA
                     TDYAYKVKALTIE"
     gene            complement(5972..6610)
                     /locus_tag="PLU55_02160"
     CDS             complement(5972..6610)
                     /locus_tag="PLU55_02160"
                     /inference="COORDINATES: protein motif:HMM:NF018751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HPJ86895.1"
                     /translation="MLTKINEQKLQIEKKLQPYYDLIVSQENYTEITKTRAELNKERT
                     KIDKERKDINKVVNDAYKDITALYDDLLTDIDTKLETSENERKAVLLEDIKLEYALSG
                     LSLPFDVFFDEKWLSRTFITNWQTDFAEKVQKVKDEIMAVKQVSDEAYNRYLECGDIT
                     KALTPTTEYSDNIAVGIPPIITIKLINPAPGQVDKVEQLLVALGVEYEINGQ"
     gene            complement(6635..7201)
                     /locus_tag="PLU55_02165"
     CDS             complement(6635..7201)
                     /locus_tag="PLU55_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86896.1"
                     /translation="MTNNIKFIDETHSYYVKLPSVSELLSKCVFPNKYKNVPKDRLKN
                     AAQFGIDLHEAIEKQSDIMLCELGQQKYQEYLELSKQFNLKVLSAEKAVYYKNLYAGT
                     YDQLCDMLGAIVLVDIKATYNLDKDWLSHQLSFYAMAIEWLLNIKIDSLWCMWLPKRK
                     KGQFVKIDRLPNELLLTIIHRYYNEHLN"
     gene            complement(7219..7890)
                     /locus_tag="PLU55_02170"
     CDS             complement(7219..7890)
                     /locus_tag="PLU55_02170"
                     /inference="COORDINATES: protein motif:HMM:NF016302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HPJ86897.1"
                     /translation="MEKNIYLKIAELRSKIKNSSLRKSGFNKFANFDYYELGDFLPLT
                     IDFEKELGLLSMFSCNSERADLIVVNCENTNEKVIFTTHVKEADLKGVIEIQKAGAEN
                     TYAKRYVYLNYLNLTEPDQVDRLDQSVSAKTPTTTTKKATSKEKQTPQVATKGVEAEK
                     TSATPRKLKEREQMIDALEKWENQEIKDKLLAFFKTNSFAEMSDNDLIRSFDRMTKEP
                     QKTKK"
     gene            complement(7931..8161)
                     /locus_tag="PLU55_02175"
     CDS             complement(7931..8161)
                     /locus_tag="PLU55_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPJ86898.1"
                     /translation="MKGGDMISLGFYIRVLRATKELTQKQLAKLSGVSESTIVAIERD
                     QRKPNDITLIKLNNVLTNGDQDILNKLFLYSN"
     gene            complement(8221..9570)
                     /gene="dcm"
                     /locus_tag="PLU55_02180"
     CDS             complement(8221..9570)
                     /gene="dcm"
                     /locus_tag="PLU55_02180"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR00675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HPJ86899.1"
                     /translation="MNDNIYQQMSLFEFVYPTYKIKKPIRLIELFAGYGSQFLALKYL
                     GANVESHKIVEWAIPAILAYKDIHYKNDKTNYAKGLTKNELVNYLYKIGVSSDYEKPM
                     TLQQLNRKSKKCLEAVYNAIKSTHNLVNIKSVAGGDLNIVNTDKYEYILTYSFPCQDL
                     SIAGNRKGMKKGGSTRSGLLWEVERILNELKDNNLELPQILLMENVPQIASSTHSKDF
                     GDWQHALQKLGYKSYCNNLIATDFGIPQTRDRTFMISILGDYFYRFPQPIKLTTRLSD
                     YLESEVDEKYYLSQKQIIGMLKTNFESYKLENKIIDRNGISKTIVKRFDGAPLVVFDK
                     DRRNHKEKLAEIVRYKNYVSWKNKKGEYNTQCNRASLVNENDNALTISTDNIPKIIFT
                     EPLRFRKLTPLECFRLMGVKDDDYSKLRSELSNSQRYCLAGNSIVVNVLMAIFNTIL"
     gene            complement(9599..9862)
                     /locus_tag="PLU55_02185"
     CDS             complement(9599..9862)
                     /locus_tag="PLU55_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86900.1"
                     /translation="MLNVIDPLLQAKQHENKIVYTRVSFFHNCFVPINSKAKITIQRN
                     NKNVLSKKVVAMFFIKDENNTINVFGVDIPNDNFFNSMFSDKK"
     gene            complement(9875..10534)
                     /locus_tag="PLU55_02190"
     CDS             complement(9875..10534)
                     /locus_tag="PLU55_02190"
                     /inference="COORDINATES: protein motif:HMM:NF017663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HPJ86901.1"
                     /translation="MENKLNQVSFIVPGKITSKGRPRARVIKSGSKGGGSYASIYTPQ
                     DTIIYENLIKMCYKEQANNYHFGNSPLSVSIYASFQAPKEVRDLRALGYGYIACFGRQ
                     DLDNIAKIVLDALNKIAFFDDKQITDLRIMKMYDKQEQLEINIYRLSNLSLAEAKELK
                     RADNIKKARIKKLKKEQRILTERIARLLNYENQTPKRKQDLQLNIDKLNIVEQELKKE
                     E"
     gene            complement(10623..11024)
                     /locus_tag="PLU55_02195"
     CDS             complement(10623..11024)
                     /locus_tag="PLU55_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86902.1"
                     /translation="MIKNLKAFDSELKALLNRRVILDNINENTLAYIEILVKEHEFVP
                     TLFDEKVRLNDFLKVVQKDTPLLYVYQVLEYVLDRELNYSYPTFINASVENHTLRDRI
                     KNEIERDLRLLIHNMFICDILDRSLIVKSTE"
     gene            complement(11065..11733)
                     /locus_tag="PLU55_02200"
     CDS             complement(11065..11733)
                     /locus_tag="PLU55_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86903.1"
                     /translation="MQKILAYTFIYAIIKIGKEVNKMTNKINNQQEKVKVIDHKSNLL
                     NKLHLEHTKKVGDCVIELLKEQTDNNDFNNIDSQQLGQKIVKKVDVLNYHYEKEKTHI
                     KKMFETPSFKRLSIMVFDNSETTCNFIIKFYRKANNNDLDNIMIAITKDNVLKRDLTE
                     NLQYALKETALAVAPFFRKGINYKLNTTYNGFHEYLTELKKIGYKVENNAHSATHFGW
                     EITK"
     gene            complement(11779..12177)
                     /locus_tag="PLU55_02205"
     CDS             complement(11779..12177)
                     /locus_tag="PLU55_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86904.1"
                     /translation="MFIIIDIDNMIDRIVNYLRPFTNLRFVFCFLIAWMITNGIWYVF
                     AFIPLKIFPNWLVWFSRSYIAFLYVPFTPEKLITIPIAIFLQRLFFKKHKKTRDQLDN
                     MYKQAHDDWAKIKSKFKRKKKTDQENKQHK"
     gene            complement(12190..>13421)
                     /locus_tag="PLU55_02210"
     CDS             complement(12190..>13421)
                     /locus_tag="PLU55_02210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPJ86905.1"
                     /translation="LVNPTDTTIVYNDIYKMTAPDGEIPTPDVSFNTATATSDTTLTL
                     NLGITYGQEFFNYRFRLMIHDKSTDFGYVKYQRDLIGSFNLGANEKGTLTFNALPFGI
                     LEFIADSSSLNLLFGDSLSDLRYSNLQYNAGIVSPNRKISELSERITTLDNPFRNKII
                     AIWGDSRESNNPTSDPSGVGDQRDTSYPALLAQKLHATVLNYGLSGGAWAENTVQQDA
                     AAAIVNRVQTEDPTASADIIIISSMNDFKLATPLGSPLPNNKDKTTFYGAMRLTYDRL
                     ATKYPNKMIWLVIPQKRYDENTDYGGGIYHQYLKAQVDVAKEYGIPTIDLYNNFPNSK
                     PTFYNTYMLNDTHFNEYGNDYVAELIARSMIGGGNSAFGFSFDLMKTSPDGNVWKLDW
                     SIDNNGNITWSPVKLTT"
BASE COUNT         3953 a         2199 c         1653 g         5516 t
ORIGIN      
        1 gcaagttcgc caaccttatt atcgacaagt cggaacgctc tcacttgatc aggcgttaaa
       61 tcatcagcta taatacatgg cacttcttta tattttaatt ttatagccgc tttaagtctt
      121 gtgtgtccag cgataatttc catattgcta tcaaggataa ttggtacttt gaaaccgaat
      181 tcttttattg agtcggcaac cgcctttact gctttgtcgt tttttctcgg attatttttg
      241 tatggtttta attcatcgat ttttttatat acaatgttta tatttttcat tgcttccccc
      301 aaaatgaccg aattgtgcca actcctcata cgatatttct cttaatttta agtcttttat
      361 aatctcttgt ggctcacatt ctttataaag cgattcaggt gcttcaatgt ttcctttatc
      421 actcactata taaatggcta acggttgttt taccccgatt gcatacgata attgaacctc
      481 gcaccatttt agattatgtt cttttaggta tctaaccgct atttgcctcg ctttatgggc
      541 tgcactaaaa tcaactttag tcgggtcttt cccgttcata caaccaccac caacattagc
      601 aaatgactga taggcatcaa ccacgatctt tcgcccggtt aatccggcat cgccatcaaa
      661 accaccaatg agaaatttac cagttggatt gaaaagatac ttatcaactt taactccatg
      721 tttttcgcaa atattatctg ctaatttttt tatgatagca tcggttttta atctatcatt
      781 ttcgctgttt tgataacata tagtgaaatc tttaatcttt ataagattaa agtcatcatc
      841 atatacgccc gttatttgtg ctttcccatc cggataaaat aaccctgata agttttgtcg
      901 caattcatca taacctttgg ctaattcttg taatataacc attgcggttg gtaaatattt
      961 ttcagtatca ttacaggcat aaccgaacat catcccgttg tcgcctgctc ctccattatc
     1021 aacacctaac gcaatatcag ccgattgtag cccgatatta ttgatgatgg tgtatttatc
     1081 gctttcgtaa cctacatcgc ttaaaacacg ctttgcgatt gctttggcgt tgaattttgc
     1141 tttagtagtt atttctccgg tgataaaaat tttaccttta ccgccgacga cttcaattcc
     1201 agcacgagtg taaggatctt gtcttaaaca agcatctaat atcgcactac ttatttggtc
     1261 acatacttta tcgggatgcc ccctgaatac gatctcattt gaatataatc tcttttccat
     1321 aataatatac actcccttta cataattttt aatatattat tttgtatcca ccacttttca
     1381 tttagttatt gttgttattt catatagata aagtgtaaaa actatatgtt atttatttct
     1441 aaactcatta taacataaaa taaagaaaca atactaattg ctaaaattac tcatacttat
     1501 caataattaa atcaattagt taaataattt aattctcatc atatctcttg gattagtagt
     1561 tgttataggt ttaccattta attttgaaaa tagatctaac tttaatggat gattaaacaa
     1621 tctaatttct ttaataatgt ttaagtaaaa gtattcacta ttctttggta tgtcttcctt
     1681 ataaattgtc atacttgtag ataagtattt attatgttta atgtttattg tcatactctc
     1741 attattaaac ttatcaaaag catttaatac ctctttagta attaacttta tataattctc
     1801 tattgtataa taacctataa ttttattttc aataacttta ttttgattaa ctataggatt
     1861 tttattattt tgaataatat aacctaattt tttatgatat attaaaccct cacttttaat
     1921 aggtcttaaa tatatataat attcacaatt atcatcatct atggttaata atggtttatc
     1981 tatagtaata aaatatgctt tttcacctct aatttgaacc aatatatctc tcgctctata
     2041 attccttaat ataaaatata tactcatata tatagtattt ctcctttctt acttattctt
     2101 attataatat ttattactat cattactaca tgtatatatt agtattgatg ctaataaagt
     2161 tatagtagac ataaatattc ttatatgaaa attagataac caggctttaa tattatcttt
     2221 atcatacatt agtaaactac taataatagg taataatacg attattgctg ttaatatata
     2281 tgatttatat tgataactat atttttgttt aatttttttg cttttcattg tcgttattat
     2341 tatttagatc ctccaatggt ttagtaaaat aagtaatagt atttattttg tttgttccta
     2401 ctggcaaaag taataacata aaagttgtaa ataatcgttt gattttagtt attgttttca
     2461 tatttaatta ttgaattacc gtacctttct ttataacgaa taattgctcg attatgtatt
     2521 cttgcaattt ctttctttat tttagcatat tgcttacgcc tatctctaat tgctttttca
     2581 ctatataagt ttttaattaa attagatact tcggcttttt tctttcgcaa atcatttaat
     2641 gtttgcaatt catcttttgt gtatattcta tgtgttttat tcattgttat nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttaatgtttg caattcatct tttgtgtata
     2821 ttctatgtgt tttattcatt gttatcgtta tttccctgct ctttccaatt aaaactaaat
     2881 aaattaaaat gctttattag ttcccattac acctcctcat aaaacataac tttactaatt
     2941 gtcgcccttg ctacatattt aataatttct ggattatata attcatacat aacggcgata
     3001 aattgtcgtt ttctttcatc ataagttgat atttcgctta cttgtgagtg tcgatatggt
     3061 ttttttattt tttcaaataa tctttcaatg tattcatcta catatccttt tgcttcatca
     3121 taactatcaa aagcaaaagt aatcaatggt agtgtcaaat catccaagta atcacgaatt
     3181 tcaacaaccc atataatttt attcattttt cctctccttt atgcgagaat tttgcatagg
     3241 agaattatta tggcaaacgc aatcaaacta attaaaaaat atttgagcca aattaacgca
     3301 tatccctctt tcttgcaatc atcgttcata attcctacct cttttcttaa tttttttcac
     3361 ctttatttgt tgtaataaat gtttcaataa atgacaacgg atcattgtaa ttaaaatcat
     3421 tatcaattaa ctttgcttgt ccgtgtttgc tgattttcct ttttactaca cttgctttaa
     3481 caagtaatcg cattgcttta cttatcattt tgttatagta atcaattgct tcttgctctg
     3541 ttcctaactt aaacttattg ttattcttaa ttataatttt atcaatttca ttactcgcgt
     3601 tcaattcatc aatgtgttga taaagcaacg gacaattaga gtaattccct ttctgcgact
     3661 taaaattata gacatgtgga aacgcacgag ctatcatttc tacatttact tcaaaaccaa
     3721 aattagtgct tatttctaaa tgttggtata aacttttgtg atgttctgtc aataatgttg
     3781 gtgtttcatt attgttattg ttgttgttat tagtattagt attcatttta ttttttcttt
     3841 cccctttctt ttaatttttc taaattgttt attaaactaa ctttgaacca agcaatttta
     3901 ttatctactt tctctggatc ataagttttt aatatattat gaactacaat tatgaattgt
     3961 ttataactaa ttggaaacaa cctatttaaa tcaatttgat aatacaattc atctaattgc
     4021 attatagcga aatcatcatc gctatttata aactttcttt ttatcagctc ttttgtcaat
     4081 gctgttttat ctcttgttaa tcttttttct aatttaatgc gtttttcttt cgcctcaata
     4141 attttatcat tattattatt tttttgagaa acaaccacaa cttcggttgt gtttatatct
     4201 tttatatctt tataatcttt attcttatct atatctaaat ctatatctaa atctatatct
     4261 aaatctatat ctgtgtttcc attttggaaa caatctgtat ccattttgga aacattgtgt
     4321 atacattttt tattattagt attattgttt aatactaatg tataggattt atttttatta
     4381 gtttttaata acgcgtattg cctttgataa ttcgtaggtg aatatcgatc tttttgtatt
     4441 gtattatgca ttttccaatg tttaataaca acaacacttg aattctcaaa agttaatata
     4501 aatctttttt gaattaaaag ttgtatatcg cctaatgttg ccccaatgtt tcgcattatt
     4561 tgtcttggtt tatcgcagaa accatcatca tctgctttca tgcacaagtg tatatataat
     4621 gcttgagctg ataatggtaa gtcgacaaac gcatcgctac ttaaaatatc gttagtaaac
     4681 atccgttttt ttgccataat aaattaaacc ctgttttgca taaagcaatt atgatttgtt
     4741 aaggttattt ttaatttcat atacattgct ttttagatcc tttcttatat ttttaatgtt
     4801 ttatttgtat tatttttaat tgttgtttat aataataatg gttattgagt atttataaat
     4861 caattttatt cataacctcc tttctcacga gcgatcggtt ttacaaataa ttgtctgctt
     4921 tcccgtaatc gctcgtgttt tttttatttt tttatttagt aatgattttg ttattaaaac
     4981 agtaaatcat catattcagc cattttatcg ctgatatcac tatttggaac tggttgttgt
     5041 tctggtgctt tcgtgttttc atcactatct tgtgtttcag ctggtttcgg ttctaagaac
     5101 aaaactctat cagcaataac ttcataagtg gtgcgttttt caccagcatg atttgtatat
     5161 cgcctttgtt ctaaaacacc ttcgataaat aacaaactac ctttcttttg gtatttagca
     5221 acattttcag ccgttttgtc ccaaaagaca acatcaataa aagttgtttc aatgtcttta
     5281 taattaccac cttgtttagg ccgatcattg actacgacac tttcagtaaa tactgctatt
     5341 ccgttttgtg ttctccttaa aattggatct cgcaccaagc gaccaacaat tacaattttg
     5401 ttatacatga tacttttata ctcctttcta ctttttatag tatatatata tatattatat
     5461 agtataaatc tattctattg taagtgcttt tactttataa gcataatcag tggctaaatc
     5521 tattaactcg ctcatttctt ttgtgttaaa agatgatgaa ccactataag cccataatgt
     5581 tattagttca ctttctttat tagttatagt atttatagtt ttaattgttt gtccggttaa
     5641 taaaatcgcc cggaattgtt gctctataag tggtaatgct tcttttgtag tggcaattaa
     5701 cactctttta atatttgttt gtttaagtag ataacaataa actttgttaa ggttttcagc
     5761 ggtcctatca ccatataatt tagttgatat actttcaatg atttgccaca aattacgatt
     5821 ttgagcttgc gttctttttg acttaatttc actaattgtt aaatcaattg ctttgtcttt
     5881 atattgttct actgccaatt tcgcaagtcg tggttcatca agtgtaaaac caattgcact
     5941 ttttccatct ggcaaataat ttatataaat attattggcc gttaatttca tactctacac
     6001 ctaaagcaac taataattgc tctactttat caacttgacc tggtgctgga ttgataagtt
     6061 taattgttat gattggtgga ataccaactg caatgttatc gctatattct gttgttggtg
     6121 taagtgcttt tgtaatatct ccacattcta aatagcgatt atatgcttca tcactaactt
     6181 gtttaactgc cattatttca tctttaactt tttgcacttt ttctgcaaaa tcagtttgcc
     6241 aattagtaat aaaagttctt gataaccatt tttcatcaaa aaacacatca aacggtaatg
     6301 acaaaccact taatgcatat tctaatttaa tgtcttcgag caaaacggct ttgcgttcat
     6361 tttcgcttgt ttctaatttt gtatcaatgt cagtaagtaa gtcatcatat aatgcggtta
     6421 tatctttata agcatcatta acgactttat tgatgtcctt tctttcttta tcaattttag
     6481 ttctttcctt atttaattcc gctcttgttt tagtaatttc agtataattt tcttgtgaaa
     6541 ctattaaatc ataataaggt tgtaattttt tttcaatttg taatttttgt tcgttaattt
     6601 ttgttaacat agtatttttt cctttctttt taagttaatt caagtgttcg ttgtaataac
     6661 gatgtattat cgttaataat aattcatttg gtaaacgatc aatttttacg aattgtcctt
     6721 tttttctttt aggaagccac atacaccata aactatcaat ttttatattt aataaccatt
     6781 caatagccat tgcgtagaac gatagttgat gacttaacca atctttatct aaattatagg
     6841 ttgcttttat atcaactaaa actatagctc ctaacatatc gcatagttga tcgtatgttc
     6901 cagcatatag atttttgtaa taaactgctt tttcagcact taatactttt aagttaaatt
     6961 gtttacttaa ttccaagtat tcttggtatt tttgttgacc taattcacaa agcattatat
     7021 cgctttgctt ttcaatcgct tcatgtaaat caataccaaa ttgagctgca ttttttaacc
     7081 tatcttttgg aacattttta tatttgtttg gaaacacaca tttacttaat aattcactta
     7141 cacttggtaa tttaacatag taagagtgtg tttcatcaat aaacttaata ttatttgtca
     7201 taaaacaata tcactttatt atttttttgt tttttgtggt tcttttgtca ttcgatcaaa
     7261 tgaacggatt aaatcattat cgctcatttc ggcaaaagaa ttggttttga aaaatgctaa
     7321 taatttatct ttgatttctt ggttttccca tttttcaagt gcatcaatca tttgttctcg
     7381 ttcttttaat tttcttggtg tagcagatgt tttttctgct tcaactccct ttgttgcaac
     7441 ttgtggtgtt tgtttttctt ttgatgttgc ttttttagta gttgtagttg gtgtctttgc
     7501 acttacactt tgatctaatc ggtcaacttg gtctggttcg gtcaagttaa gataatttaa
     7561 gtaaacatat ctttttgcat aagtgttttc tgcaccggct ttttgaattt caataactcc
     7621 tttcaaatca gcctctttta catgtgtcgt aaaaataact ttttcgtttg tgttttcaca
     7681 attaacaacg attaaatcgg ctctttcact attacaacta aacatactta ataaccctaa
     7741 ttctttttca aaatcaattg ttaaaggtaa aaagtcgcct aattcgtaat aatcaaaatt
     7801 agcgaacttg ttaaaaccac tctttcttaa cgatgagttt ttaatttttg atcgtaattc
     7861 agcaattttc aaataaatgt ttttttccat attttcctcc tttcatttat tttatttatt
     7921 tgcattatgt ttaattagag taaagaaata atttatttaa gatatcttga tcgccgtttg
     7981 ttaacacatt attaagtttg ataagtgtta tatcgtttgg ctttctttga tctcgctcaa
     8041 tagcaactat ggtgctttca ctaacaccag atagtttagc aagttgcttt tgtgtgagct
     8101 cttttgttgc tcttaatact ctaatgtaaa atccaagact tatcatgtcg cctcctttca
     8161 ttttcctaat ttcattatac tacatataaa agtaaacgca agtaaaatta ttaaaaactt
     8221 ttatagaatt gtattaaaaa ttgccataag cacattaacg actatgctat taccagccaa
     8281 acaatatctt tgtgaattgc ttaattcact tcttaactta ctataatcat catcttttac
     8341 tcccattagt ctaaaacact ctaaaggtgt taattttcta aatcgtaaag gctcagtaaa
     8401 tataatctta ggtatattat ctgttgaaat tgtaagtgcg ttatcatttt cattaactaa
     8461 tgaagcacga ttacattgtg tgttatattc accctttttg tttttccaac taacataatt
     8521 tttataccta acaatttcag ccaatttctc tttatggttt ctgcggtctt tatcaaaaac
     8581 aacaagaggt gcaccatcaa accttttcac aattgtttta cttattccgt ttctatctat
     8641 tattttattt tctagtttat aactctcaaa attggtttta agcataccta taatttgctt
     8701 ttgtgataaa tagtattttt catcaacttc gctttccaaa taatcgctta atcgtgttgt
     8761 taactttatt ggttgtggaa accgataaaa ataatcacct aaaatactaa tcataaaagt
     8821 tctatctctc gtttgtggaa taccaaaatc ggtcgcaatt aaattattac aataactttt
     8881 ataacctaat ttttggagtg cgtgttgcca atcgccaaaa tctttactat gcgttgaact
     8941 tgcgatttgt gggacatttt ccattagtaa gatttgtggt aattctaaat tattatcttt
     9001 taattcatta agtatgcgtt ctacttccca taaaagacca ctacgagttg agccgccttt
     9061 tttcataccc ttacgatttc cagcaattga taaatcttga cacggaaacg aataagttaa
     9121 aatatattcg tatttatcag tattcacaat gtttaagtcc ccccccgcca cactcttaat
     9181 gttaactaaa ttatgagtgc ttttaattgc attataaact gcctctaaac actttttgct
     9241 ttttctattt agttgttgta aagtcatcgg cttttcataa tcgctactaa caccaatttt
     9301 atataaatag ttaaccaatt catttttagt aagacctttt gcataattag ttttatcatt
     9361 tttgtaatga atatctttat aagccaaaat tgcgggtata gcccactcga caattttatg
     9421 actttcaaca ttagcaccta aatatttaag tgctaaaaac tgcgaaccat acccagcgaa
     9481 taattcaatt aacctaatcg gttttttaat tttataagtt gggtaaacga actcaaataa
     9541 gctcatttgt tgataaatat tatcgttcat gcttattaac aaccttttta actacttttt
     9601 attttttatc actaaacata ctattgaaaa agttgtcgtt tggaatatca acaccaaaaa
     9661 catttattgt attgttttca tctttgataa aaaacattgc gacaactttt ttacttaata
     9721 cattcttgtt gtttctttga atagtaattt ttgctttact atttattgga acaaaacaat
     9781 tgtgaaaaaa tgatactctt gtatatacta ttttattttc gtgttgttta gcctggagta
     9841 atggatctat tacatttaac ataaacaatt tttattattc ctcctttttt aattcttgtt
     9901 ctacaatatt taatttatca atatttaatt gcaaatcttg ttttcttttt ggtgtttgat
     9961 tttcataatt taataatctt gctatgcgtt cagttaaaat gcgttgttct ttttttaact
    10021 ttttaattct cgctttttta atattatctg ctcgtttaag ctcttttgct tctgctaatg
    10081 ataaattact taaccgatat atgttaattt ctaattgttc ttgtttatca tacattttca
    10141 taatgcgtaa gtcagtaatt tgtttatcat caaaaaacgc aattttgttt aatgcatcta
    10201 aaacaatttt ggcgatattg tctaaatctt gcctaccaaa acaagcaata tacccatagc
    10261 ccaacgctct taaatctctt acttcttttg gagcttgaaa cgaagcgtaa atacttacac
    10321 tcaatggaga attaccaaag tgataattat ttgcttgttc tttataacac attttaatta
    10381 gattttcata aataatagtg tcttggggag tgtaaatact cgcataacta ccacccccct
    10441 tactaccact ctttattact cttgctcgtg gtctaccttt acttgttatc ttacccggca
    10501 cgataaaact tacttgattt aatttatttt ccatatttgc ccctttcttt gcttatggtt
    10561 tggtttttaa tattaagtta gtgtatttat acctttttat tagtttgttg cttaaaactc
    10621 gtttattcag tgcttttaac aataagtgaa cgatctaaaa tgtcgcagat aaacatattg
    10681 tgtattagta gtcttaaatc tcgttcaatt tcatttttaa tgcgatctct taatgtgtga
    10741 ttttcgacac ttgcgtttat aaatgttgga tatgaataat taagttctct atccaacaca
    10801 tattctaaca cttgataaac ataaagtaat ggtgtgtctt tttgaactac tttcaaaaaa
    10861 tcatttaatc ttactttttc atcaaataat gttggcacaa actcatgctc tttaacgagt
    10921 atttcaatat aagcaagtgt attttcattt atattatcta aaattactcg cctatttaat
    10981 agtgctttaa gttcactatc aaatgctttt aagttcttaa tcatttttat ttatttgatc
    11041 cttctttctc gaattatttt tttattattt tgtaatttcc caaccaaaat gcgtggcact
    11101 atgtgcgtta ttttcaactt tataaccaat cttttttaat tcagttaaat attcatgaaa
    11161 accattataa gttgtattta acttatagtt aattcccttt ctaaaaaacg gtgcaacggc
    11221 taatgctgtt tcttttaatg catattgtaa gttttcagtt aaatctcttt ttaagacatt
    11281 atcttttgta atcgcaatca taatattatc taaatcatta ttatttgctt tcctataaaa
    11341 cttaattata aaattacaag ttgtttcgct gttatcaaac accataattg ataatcgctt
    11401 aaagctcggt gtttcaaaca tttttttaat gtgcgttttt tctttttcat aatgataatt
    11461 taagacatct acttttttaa caattttttg tcctaattgt tgactatcaa tgttattaaa
    11521 atcattatta tcggtttgtt cctttaataa ttcaataaca caatcgccta cttttttagt
    11581 gtgttctaaa tgcagtttgt ttaatagatt acttttgtga tctattacct ttactttttc
    11641 ttgttggttg ttaattttgt ttgtcatttt atttacctct tttcctatct ttattatagc
    11701 ataaataaaa gtatatgcaa gtattttttg cattttcttt tacttttttt aataaaaagg
    11761 actaattgat agtcctaatt acttatgttg tttattttct tgatccgttt tcttttttcg
    11821 tttaaacttt gatttaattt tagcccaatc atcatgagct tgtttataca tattatctaa
    11881 ttgatccctt gttttcttat gtttcttaaa gaataatctt tgtaaaaata ttgcaatagg
    11941 aattgtaatt aacttttctg gcgtaaatgg aacatataaa aatgctatat aactgcgact
    12001 aaaccaaaca agccaatttg gaaatatttt aagtggaata aaagcaaaaa cataccagat
    12061 cccatttgtt atcatccaag caatcaaaaa acaaaataca aatcttaaat tagtgaacgg
    12121 ccgcaaataa ttaacaatgc gatcgatcat attatctata tcaattatga taaacataaa
    12181 attaagttat taagtagtca acttaacggg cgaccaagta atgttgccgt tattgtcaat
    12241 agaccaatct aacttccaaa cattgccatc tggacttgtt ttcattaagt caaaactaaa
    12301 gccaaatgca ctattgccac caccaatcat tgagcgagcg attaactctg ctacataatc
    12361 attaccatac tcgttaaagt gcgtgtcatt taacatatat gtattgtaga aagtcggctt
    12421 actatttggg aagttgttat aaaggtcaat agtaggtatg ccatactctt tagcaacatc
    12481 gacttgtgct tttaagtatt ggtgatagat gccgccccca tagtctgtgt tctcatcata
    12541 gcgtttttgc ggtattacaa gccaaatcat tttgttagga tactttgttg ctaacctatc
    12601 ataagtaagt cgcattgctc cataaaacgt agtcttatct ttattattag gtaatggact
    12661 acctaatgga gtggctaact taaagtcgtt catcgaacta atgataatga tatcagccga
    12721 cgcagttggg tcttccgttt gcacacggtt gacaattgca gccgccgcat cttgttgaac
    12781 tgtattttca gcccaagcac cgccacttaa gccatagttt aatacggttg cgtgtaattt
    12841 ttgagcgagc agtgctggat aacttgtatc tctttggtcg cctacaccac ttgggtcgct
    12901 tgtaggatta ttactctcac gactatctcc ccatatagca atgattttgt ttctaaacgg
    12961 attatcaaga gtagtaatgc gttcgcttaa ttcactaatc ttgcgatttg gacttacaat
    13021 gccagcgtta tattgtaaat tactatatct taaatcgctt aaactatcgc caaagagtag
    13081 gttaagtgaa ctactatcag cgatgaattc aagtattcca aaaggtaaag cattaaaagt
    13141 taatgttcct ttttcattag cacctaaatt aaacgaaccg attaaatctc tttggtattt
    13201 gacatagcca aaatcagttg acttgtcgtg tatcattagt ctaaatctgt agttaaaaaa
    13261 ctcttgtccg tatgtaattc ctaagttaag agtaagtgtt gtatcgcttg ttgctgtcgc
    13321 tgtattaaac gatacatcgg gagttggtat ttcgccatct ggtgctgtca ttttatatat
    13381 atcattatag actattgtag tatcggttgg attgactaat t
//
LOCUS       DAOPCH010000019        13331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_4638_length_13331_cov_4.772898, whole genome shotgun sequence.
ACCESSION   DAOPCH010000019 DAOPCH010000000
VERSION     DAOPCH010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 13331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13331
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4638_length_13331_cov_4.772898"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLU55_02215"
     rRNA            <1..711
                     /locus_tag="PLU55_02215"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02540"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02540"
     gene            820..1047
                     /locus_tag="PLU55_02220"
     CDS             820..1047
                     /locus_tag="PLU55_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86906.1"
                     /translation="MKIGHYILIFLVIGAIVGFALGLYYQPSCPIDPSTGMRTECMGD
                     PRSYGITGAILGALIGTVIGLILGKIVKKKK"
     gene            1194..1544
                     /locus_tag="PLU55_02225"
     CDS             1194..1544
                     /locus_tag="PLU55_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPJ86907.1"
                     /translation="MKVYVGNLPFSVDDEKLKELFSSYGEIEEVSVIKDKFSGRSKGF
                     GFVTFKNDDEAKKAIEEMNDKEVEGRALKVNEAKPLDPDAPRKPRRNFGGRRRDSRGP
                     RRDFGNSDGNRRRF"
     gene            complement(1677..1868)
                     /locus_tag="PLU55_02230"
     CDS             complement(1677..1868)
                     /locus_tag="PLU55_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86908.1"
                     /translation="MAKVVNAKKEKHKEEKGALFIPAGLFLGMGWGFLTGLFVAGLFM
                     GLGIGFLAFAIVNLLSKKK"
     gene            2297..2368
                     /locus_tag="PLU55_02235"
     tRNA            2297..2368
                     /locus_tag="PLU55_02235"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2329..2331,aa:Phe,seq:gaa)
     gene            2420..2749
                     /locus_tag="PLU55_02240"
     CDS             2420..2749
                     /locus_tag="PLU55_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86909.1"
                     /translation="MKVKNRDSKEADAYILGTVSLILALFIPFSALLLKIFENNSKIS
                     SIIISAGIPFLAVILGIFGLIKSLKCDGKRAKKAKIFSILSILIGFFLVAINLYTFIK
                     YSVIGIR"
     gene            2753..3313
                     /locus_tag="PLU55_02245"
     CDS             2753..3313
                     /locus_tag="PLU55_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86910.1"
                     /translation="MVMKNMKSKRAAMEMSVGTIVTIVLLMSALVLGLILIKIMHKSS
                     ANAINSIDEGVRNQLDQAFQKDDSKKVVISPGRTVSVKKGGEDAGFGFSIRNIGATAT
                     FSYTIKYMDSDCSTTANVANKLISLGSTGQGISIPGGNVMDNPILVKFRIPEDFPPCE
                     IRYNLEVKRGASVYDTVQVDIRVVSR"
     gene            3341..3889
                     /locus_tag="PLU55_02250"
     CDS             3341..3889
                     /locus_tag="PLU55_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86911.1"
                     /translation="MKRKTKIIILVIVSLVIIAILVNELILKEGVERDPAKERVLPLT
                     EQQSANEIGKAFGSDEDKSAIYPKSRSLIIKQGQEEAIALGIKNLLENSSDVSIFSYN
                     IIVAESTCGDENVLDWISNGKSEENIPISFGDIYATKIHILIPKKAPPCTVRYHIEIK
                     NEIDGIYQEYDNILFTVTSKAR"
     gene            3905..4396
                     /locus_tag="PLU55_02255"
     CDS             3905..4396
                     /locus_tag="PLU55_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86912.1"
                     /translation="MKIKEIILGIAIAIIFLMFCVFGTKLIYDEPKYEDYCDYQEFSE
                     TDYINESYYTQVYRECSDKYNEANKDYSKKMFIISLIFGILVIVGCTIFISTNSISGG
                     LMFGSLMFIIYGTGRYWNYMDDLVRFIILSIALVVLIYVSYWTSKKMKDGNKRTRKSE
                     KRK"
     gene            4441..4632
                     /locus_tag="PLU55_02260"
     CDS             4441..4632
                     /locus_tag="PLU55_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86913.1"
                     /translation="MQDKIEENEEYFIPEERSKSKIKNEETFNPKCRCPELSCPRHSN
                     CKECKAFHSKRMELTYCGK"
     gene            4721..5986
                     /gene="rbcL"
                     /locus_tag="PLU55_02265"
     CDS             4721..5986
                     /gene="rbcL"
                     /locus_tag="PLU55_02265"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018153747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III ribulose-bisphosphate carboxylase"
                     /protein_id="HPJ86914.1"
                     /translation="MQNKFLNLKYKPKKTDVICQYKITPSKGYTLKKVSEEVAGESSI
                     GTWTNIGTMNKKVAEKLSPKIYYMNEKEKRVRIAYPIELFELGNVPEILSSIGGNIYG
                     MKSVKGLMWEDLRIPEKMLKSFKGPRYGIYGLRKLMKIPKRPLVGTIIKPKVGLDEKN
                     HARVAYEAWIGGCDIVKDDENLTNQNFNNFKKRFLLTIKACKDAEKKTGEKKAYLINC
                     TAESEEMKRRIKFVEENGGNYIMLDILTLGWGALQTARNFTKLPIHAHRAGHAIYDRN
                     PDHGMSMEVIAQLARMVGVDTLHIGTVYGKMTGNKEEILHIQEEIEMQFTPQTKRNLE
                     QRWYNIKPILGVASGGVYPGIVPKILKAMGNNIVVQAGGGVHGHPDGTIAGAKAMRQA
                     IDAFMEKQSLKKYSENHEELKRALENWGE"
     gene            6023..6322
                     /locus_tag="PLU55_02270"
     CDS             6023..6322
                     /locus_tag="PLU55_02270"
                     /inference="COORDINATES: protein motif:HMM:NF039182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HPJ86915.1"
                     /translation="MERLRAKKIKERAKTGVKVFKREFKKQMSTAILAAFGFLIALVW
                     KDVVTETVTKLSGNATLGGTIIGAIIVTIICVLGIMIVSRMLKEKEVVKDEQTNK"
     gene            6303..6806
                     /locus_tag="PLU55_02275"
     CDS             6303..6806
                     /locus_tag="PLU55_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPJ86916.1"
                     /translation="MNKQTSKKINNKKNKIKDEKYRKAVFAVTYYITPEGKIEYLILK
                     RKKHWKGWEFPKGKIEKFETKKAAARREVNEETGLKVLKVKRFKEHGSYLYLKKIWDR
                     PDKIGQTYHLFAVQVEKGDGKLKLDPIEHNGHKWASFNEAIKILTWPNQKKCMKIVND
                     WLIKKKR"
     gene            6838..7233
                     /locus_tag="PLU55_02280"
     CDS             6838..7233
                     /locus_tag="PLU55_02280"
                     /inference="COORDINATES: protein motif:HMM:NF017482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HPJ86917.1"
                     /translation="MKPREYILDSGTKIFLGKNSENNDELVKSYCGKPNLILHTAEPG
                     SPFCVIDKIKPKPTKQEIKISAIICASKSQDWRDNKGDVKVHQFTGKEVYKKKFMKSG
                     TWGINKKPKIIKVKKKEIEEYIEKNKIKE"
     gene            7236..7520
                     /locus_tag="PLU55_02285"
     CDS             7236..7520
                     /locus_tag="PLU55_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HPJ86918.1"
                     /translation="MPAIRQIQLGKNGVTEQFISNLKHHFNNTKNVKVSVLKSCCRDR
                     EELREINEKILRELGNNYTSRIIGYTIVIKKWRRDRRENLKENPEEEKEV"
     gene            7630..8067
                     /locus_tag="PLU55_02290"
     CDS             7630..8067
                     /locus_tag="PLU55_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="40S ribosomal protein S19"
                     /protein_id="HPJ86919.1"
                     /translation="MENPVYDMRAQEYNIKLAEALKKIPEFNEPEWAKFVKSGAGKER
                     PIDDEDFWHKRAASVLRCIYKKGSIGVQRLRTKYGSKKDRGFKPEKFSKGSGKIIRTI
                     LQQSDKAGFTEIQKPIRGVRSKKPGRILTDKGKKFLEGIGGGK"
     gene            8064..8324
                     /locus_tag="PLU55_02295"
     CDS             8064..8324
                     /locus_tag="PLU55_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86920.1"
                     /translation="MIEYSLMKNNKPLTIDELMEVSGVVGALFQETETHKMERTVRKT
                     YGVNGQAFHIYYNERASKIFLPEKYEKQIRNMLSEKGGLKCL"
     gene            8315..8518
                     /locus_tag="PLU55_02300"
     CDS             8315..8518
                     /locus_tag="PLU55_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86921.1"
                     /translation="MPIKNKNKKRILAKKGRQTKWAPVWAVLKKYGTGKRVHPSIMTK
                     YKRSWRRTKLHVKPGKRRKSHFG"
     gene            8511..8993
                     /locus_tag="PLU55_02305"
     CDS             8511..8993
                     /locus_tag="PLU55_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86922.1"
                     /translation="MVKMTLEENPRYKKYCVYSNMKSEITGMDLPKSDEPDHKIFIIN
                     GKYDFNGLDLSSYNWKKSNTITPQQKKFLETTTGYKDYKENVFYQGPQGNVMMHLNYK
                     TDIHKLKVNLNKASRDTMKAVKSMFELELGCICYEEDNLPEGLLEKIRDIERAKAKLN
                     "
     gene            8995..9576
                     /locus_tag="PLU55_02310"
     CDS             8995..9576
                     /locus_tag="PLU55_02310"
                     /inference="COORDINATES: protein motif:HMM:NF002258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31e"
                     /protein_id="HPJ86923.1"
                     /translation="MANKKSENKIEREYVIPLREKSRVVARYKKTPKAIKTIKEFLAK
                     HMKVENRDLNKIKIDKDLNQFLWARGIRKHPHKIKVKAIKKGENIIVQLVDYPDNLKF
                     KKLREEKARKAALENIESKKGMMQKMKESLQKPEEKDENKDGVDDKKEIAEKEKATVE
                     ANQKFQKEKAKQAKHQTEKPKKAVKPVRVALQK"
     gene            9670..10377
                     /locus_tag="PLU55_02315"
     CDS             9670..10377
                     /locus_tag="PLU55_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPJ86924.1"
                     /translation="MLNRLKKEIEEAKNPEQAKILQRFFKTGKGEYGEGDIFLGIKVP
                     IQRKIAKGYTNLSFMDLQTLLNSKIHEERLIALIILTNKYQKSKKDFVKKRQIFEFYL
                     KNTHNINNWDLVDLSAPNIVGDFSSTDGTEVIRFLAKSKNLWERRIAIVSTYAFIKKR
                     IFGETLAIADMLLKDEHDLIHKAVGWMLREVGKRNQEVLEIFLKERYKTMPRTMLRYS
                     IEKFPEEKRKDYLKGEI"
     gene            10493..11365
                     /locus_tag="PLU55_02320"
     CDS             10493..11365
                     /locus_tag="PLU55_02320"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF003280.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HPJ86925.1"
                     /translation="MNIEEIKNKKTIKELLEFGIINLDKPSGPTSFSVSAFTAKRLKL
                     RKTSHFGTLDPKVTGVLPVALNRACKLTGYFLGEDKEYIGVMRIHEDIDIEIIKKAIQ
                     EKFIGKIIQMPPVKSRVKRQNRPREIYSFELLQKKGRDILFRVKCEGGTYIRKLIDDL
                     GKETKIGAHMLELRRLSAGIFDETYSVNMYEFEKAVEEYEKGNEKFLREIIIPAEIVG
                     RVYPVMNIREDNLKQILTGKPIYKKDLIEDEGWKVDSIVSCFCNGNFVGMYKIFRGKE
                     IFAKPEFVMQEIRG"
     gene            11367..11966
                     /locus_tag="PLU55_02325"
     CDS             11367..11966
                     /locus_tag="PLU55_02325"
                     /inference="COORDINATES: protein motif:HMM:NF015946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86926.1"
                     /translation="MRQFVYFSSSAATSGSALSKYSQYDGDLMKAGRMDIAIHSFIQG
                     VFLSHDFRKDCKFHFVFYGMPDPPKHIEIQVKDETQISKKDVGNMIKKILYKYKEKEK
                     TEVFPGCFVEKKSFLSVIEELIGQGNEIFILDKKGEDIRKINIPKNCVFIIGDHEGLP
                     KKELKRLKNYCKPVSIGNKMYFASQTVAVVNNELDYRNV"
     gene            12019..12294
                     /locus_tag="PLU55_02330"
     CDS             12019..12294
                     /locus_tag="PLU55_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPJ86927.1"
                     /translation="MKKAVRMYIDGTIQDVFFRGFVKENAERHNVKGFVRNLEDGRVE
                     IFLEGNIDDVNKVVELCKKGPKHSQIKGIDMRPEKFQDFKTFKILHI"
     gene            complement(12291..12755)
                     /locus_tag="PLU55_02335"
     CDS             complement(12291..12755)
                     /locus_tag="PLU55_02335"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HPJ86928.1"
                     /translation="MKKILKFPEGNKQINLSSCGPNAMKQVILYKIGLDIPEYQLINM
                     SCCSTETGTPVRGMIQISKNFNLNHKLKHNASIDDIIYSINTKNPVILLIQEWKSGHY
                     VVANGYDTKHEKVFYYDPLYGKTRSINYSTLNKNWHGLDVFHRNRMGIFFED"
     gene            12816..13250
                     /locus_tag="PLU55_02340"
     CDS             12816..13250
                     /locus_tag="PLU55_02340"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HPJ86929.1"
                     /translation="MIYTNSDGGARGNPGPGAVAALVRKEGEIITKYSKFIGENTTNN
                     VAEYEGLILALELASNITKDEITCFLDSELVVKQLLGEYKVRNKNILPLFLKVQKLQE
                     RFKKIKYAHVPRTDNFQIIVDEMLNEELDNKGYRKNRFRRRK"
BASE COUNT         5483 a         1542 c         2441 g         3865 t
ORIGIN      
        1 aaccttgtaa tactacccac tttaattaaa tttgcttact ctttagagga cagcagcagg
       61 cgggcagttt gactggggcg gtgccctgtc gaaaagatat cgataggacc caaagacaag
      121 ctcacctagc ttggaaatct agggatgagt gcgagggcaa aagcttgtct aactgtattt
      181 tgaatagcaa gaaatgcaga taggaaacta gggcctagtg aacccacgtg cttttttaat
      241 aggagccgtg tctgatagaa aagttacccc aaggataata agctggtcgc ccccaatagt
      301 ccgaattgac ggggcggttt ggtatttcgc tgtcggctct ccctaacctg cgcgtgcagt
      361 agcgtgcaag ggtgtcggag ttcacgcatt aaaagggatc gttagctggg ttaagaacgt
      421 cgcgagacag tttgtataat atctactagg tgtgttattg tctgagtgga acaactttct
      481 agtacgagag gaacggaagt tgggtgcctc tagtgagcag ttgttatatg gcaggctgca
      541 tagccacgca ctacatggat aagagctgaa tgcatctaag ctcgaagcca tccgcaaaac
      601 gagacaatta aggccgcaaa agaagattgc gttgatagag ctcttgtgta agattcaagc
      661 tcacgcgaga atttcagcat agagctacta atagcttcat ttgtatgttt gaagatttca
      721 agattttctg ttacttattt atttgatttt atttctgata acaactaact gttttgaagt
      781 tataacaaca tttttaaatc taaattacta atttaactta tgaaaatagg ccattacatc
      841 ctaatattct tagttatagg ggcaattgtt ggattcgctc tgggattata ctatcaaccc
      901 tcttgtccaa ttgacccttc aacaggtatg agaactgaat gtatgggaga cccaagaagt
      961 tatggaatta caggagcgat acttggtgct cttataggaa cagttattgg attaattcta
     1021 ggtaagatag taaaaaagaa aaagtaaatt taatttgcac attctccaaa gcatttaaaa
     1081 accaattgtt tgtatttatt ttgaacgtag aatgaataga ttcagtgatt cagtttgatt
     1141 tcgcggttcg attagttcta agttagatat gagtgtaagg aggatataag gacatgaaag
     1201 tttatgttgg aaatctacca tttagtgtag atgatgaaaa gctaaaagaa ctattttcat
     1261 cttatggtga aatagaagaa gtctctgtaa tcaaggataa attttctgga agatctaaag
     1321 gatttggttt tgttacattc aaaaacgacg atgaagctaa aaaagcaatc gaagagatga
     1381 atgataaaga agttgaaggc agagcattaa aagttaatga agctaagcca cttgaccctg
     1441 atgccccaag aaaaccaaga aggaattttg gtggtagaag aagagattca agaggtccta
     1501 gaagagattt tgggaacagt gacggaaata gaagaagatt ttaattaaaa atcaattcta
     1561 tataaattca aaccaattta ttttttattt ttaaaattta ttttatcttg aaaacttgat
     1621 ttttctataa gtaaacaaat tagaaaaatt caaacattaa atttttcata ttaattttat
     1681 tttttcttac tcaataaatt aacaattgca aaagccaaaa aacctattcc aagacccata
     1741 aaaagccctg caacaaagag acctgtaaga aaaccccacc ccatacctaa aaacaatcca
     1801 gctgggataa acaaagctcc tttttcttct ttatgttttt ctttttttgc attcaccact
     1861 tttgccatga taagaacaag aagataaaac ttaaaaaccc ttctaattac ttattttagt
     1921 aagtcaggta gttaacatcg ggactttggc attaaattgt caagaaatcc aattatatga
     1981 tttctaagtt ctgaggaagg tccggacacc cttaacaatt agttaagaaa aagttgttgg
     2041 agaaattcca attgtcgtga ggcaaagctc tgatatagag acgagcgtat taagttacgg
     2101 tggaacgaac atctcaactg gtgcaaggca tagccgcata gtggaatgtt aacatgaact
     2161 gtctgtcttg aaaaagaaac agagttcaac agaatctggc ctattcctga cttacatttt
     2221 ttattattta attattttca aaaataattt tacaataatt ctaaacattt aaaagtataa
     2281 aacattactc taaattgggg ctttagctca gcgggagagc acatgactga agatcatggt
     2341 gtgccgggtt cgactcccgg aagccccatt tctattagaa ataaaaacat ttataattaa
     2401 taactcaata aaaatataaa tgaaggtgaa aaatcgtgat tctaaagaag cagatgcata
     2461 tatacttggc acagtaagtt taattcttgc tttatttatc ccattttcag cactcttgtt
     2521 aaagatattt gaaaacaaca gtaaaatttc ttcaataata attagtgcag gaattccatt
     2581 tttagctgtg attcttggaa tatttggatt aattaaaagt ttaaaatgcg acggtaaaag
     2641 agcaaaaaaa gcaaaaatat tcagtatcct ttcaatatta ataggatttt ttttagttgc
     2701 gataaattta tatacattta taaagtatag tgttataggt ataagataaa agatggtgat
     2761 gaagaacatg aaatccaaaa gagcagcaat ggagatgagt gttggaacaa tagttactat
     2821 agttctttta atgtctgctt tggttttagg gttaatactt ataaagatta tgcacaaaag
     2881 ttctgcaaat gctataaata gcattgatga aggagttaga aaccaattag accaagcttt
     2941 tcaaaaagat gattccaaga aagttgttat ttctcctgga agaacagtct ctgtaaagaa
     3001 agggggagag gatgcagggt ttggattctc aattagaaac ataggggcaa ctgcaacatt
     3061 ttcatatact ataaaatata tggatagtga ctgcagtaca actgctaatg tagcaaataa
     3121 acttatttct ttaggaagta ctggacaagg aatatcaatc cctggtggaa atgtaatgga
     3181 taatccaata ttagtcaagt tcagaatacc agaagatttt cctccttgtg aaataagata
     3241 taacctagaa gttaaaagag gggctagtgt ttacgataca gtacaagtag atattagagt
     3301 agtctctaga taagctaatt tttaataata gcaatataaa atgaaaagaa aaactaagat
     3361 tataattctt gtaatagtat ctttagtaat tatagctatt ttagtaaacg aattaatttt
     3421 aaaagaaggt gttgaaagag accctgccaa ggagagagta ttacctttaa cagaacaaca
     3481 gtcagcaaat gaaattggaa aagcttttgg ttcagatgaa gataagtcgg caatatatcc
     3541 taaatcaaga agtttaataa taaaacaagg acaagaagaa gctattgcac ttgggataaa
     3601 aaacttatta gaaaattctt ctgatgtttc aatattttct tataatatta ttgttgctga
     3661 gagtacatgt ggtgatgaaa atgtattaga ttggatttca aatggaaaat ctgaagagaa
     3721 tattcctata agttttggag atatttatgc cacaaaaata cacattttaa tccctaaaaa
     3781 agctccaccc tgtactgtta gataccatat agaaattaaa aatgaaattg atggaatata
     3841 tcaggaatat gataacatac tttttacagt aacatctaaa gcaagataaa cataaatcta
     3901 taaaatgaaa ataaaagaga ttatacttgg aattgcaata gcaataatat ttttgatgtt
     3961 ttgtgttttt ggaacaaaat taatatatga tgaaccaaaa tatgaagatt attgtgatta
     4021 tcaagaattt tcagaaacag attatatcaa tgaatcttat tatacacaag tttatagaga
     4081 gtgttcagat aaatataatg aagcgaacaa agattattct aaaaaaatgt ttataataag
     4141 tttaatattt ggaattttag ttattgttgg atgtacaata tttataagta caaattctat
     4201 ttcgggggga ttgatgtttg ggagtttgat gtttattatt tatggaacag gcagatactg
     4261 gaattatatg gatgatttgg taagatttat aattttaagt attgcattgg tagttcttat
     4321 atatgtctct tactggacaa gtaaaaaaat gaaggatgga aacaaaagaa caaggaaaag
     4381 tgaaaagaga aaataaacta aatcaataat tttttaaatt aacgagtttc taaataattt
     4441 atgcaagaca aaatagaaga aaatgaagaa tattttattc cagaagaaag gtctaaaagc
     4501 aaaataaaaa atgaggaaac ctttaatcct aaatgtagat gtcctgaatt atcttgtcca
     4561 aggcattcta attgtaaaga atgtaaagcg tttcatagca aaagaatgga attaacttat
     4621 tgtggaaagt aatttaattg aaattcaaaa acaaataaga ataatttaaa aaccaaaaat
     4681 attttagtaa tattgttaat gtaaaagagg taaaatagtg atgcaaaata aatttcttaa
     4741 tttaaaatac aaaccaaaga agacagatgt tatttgtcag tataaaataa ctccctcaaa
     4801 aggatatact ttaaaaaaag tttctgaaga agttgctgga gagagctcta ttggaacatg
     4861 gacaaatatt ggaacaatga ataagaaagt tgctgaaaaa ttatctccga aaatatatta
     4921 tatgaatgaa aaagaaaaga gagtgagaat tgcttatcct attgaattgt ttgaattagg
     4981 aaatgttcct gaaatactct cgagtattgg aggaaatata tatggaatga aatcagtaaa
     5041 agggcttatg tgggaagact tgagaattcc tgaaaaaatg ctgaaaagtt ttaagggtcc
     5101 aagatatgga atttatggat taagaaaatt aatgaagatt cccaaaagac ctttggtggg
     5161 aacaataata aaaccaaaag ttggacttga tgaaaaaaat catgcaagag tcgcttatga
     5221 agcatggatt ggaggatgtg atattgttaa agacgatgaa aatttaacta atcagaattt
     5281 taataatttc aaaaaaagat ttttattaac tatcaaggca tgtaaagatg ctgagaaaaa
     5341 aacaggagag aaaaaagcat acttaattaa ttgcactgca gaatctgaag aaatgaaaag
     5401 aagaataaaa tttgttgaag agaatggagg gaattatatc atgttggata tattaacatt
     5461 aggatgggga gcattgcaaa ctgcaagaaa ttttacaaag cttcctattc atgcacatag
     5521 ggcaggacat gcaatatatg atagaaatcc agaccatggg atgtctatgg aagttattgc
     5581 tcaattagca agaatggttg gggtagatac attacatatt ggaacagtat atggaaagat
     5641 gacaggaaac aaagaagaaa ttcttcatat acaagaagaa attgaaatgc agtttactcc
     5701 tcaaacaaaa aggaatttgg aacaaagatg gtataatatc aaacctattt taggagttgc
     5761 atctggagga gtttatcctg gaattgtacc taagatttta aaggcaatgg gaaataatat
     5821 tgtggttcaa gcaggaggtg gagttcatgg acatcctgat ggaacaattg ctggagcaaa
     5881 agcaatgaga caagcaattg atgcatttat ggaaaaacaa tcacttaaaa aatattctga
     5941 aaaccatgaa gaattaaaaa gagcattaga aaattgggga gaataaataa taaagagatt
     6001 aacaaaaaca taaataattg aaatggaaag attaagggca aaaaaaataa aagaaagagc
     6061 aaaaacagga gtaaaggtat ttaaaagaga attcaaaaaa caaatgagta ctgcaatact
     6121 agcagcattt ggttttttaa ttgctcttgt ctggaaagat gttgttacag aaactgttac
     6181 taaattatct ggtaatgcca cattaggagg aacaattatt ggagcaataa ttgtaactat
     6241 tatatgtgta ttgggaataa tgatagtttc aaggatgtta aaagaaaagg aagttgttaa
     6301 agatgaacaa acaaacaagt aaaaaaataa acaataaaaa aaataagata aaagatgaaa
     6361 aatacagaaa agctgtattt gcagtaactt attatataac tcctgaagga aaaatagaat
     6421 atttgatttt aaaaagaaaa aaacattgga aaggatggga gtttccaaaa ggtaagattg
     6481 agaagtttga aacaaaaaaa gcagctgcaa gaagagaagt aaatgaagag acaggattaa
     6541 aggtattaaa agtcaagaga tttaaggaac atggaagtta tctttatctt aaaaaaatat
     6601 gggatagacc tgataaaata gggcagacat atcatctttt tgctgtgcag gttgaaaaag
     6661 gagatggaaa attaaagctt gacccaattg aacataatgg acacaagtgg gcttctttca
     6721 atgaagcaat taaaatactt acttggccaa atcaaaaaaa atgtatgaaa attgttaatg
     6781 actggttaat taagaagaaa agataaaatc taaaaacata tttttattaa aagatatatg
     6841 aaaccacgag aatatatcct tgattctgga acaaaaatat ttttaggtaa aaattctgaa
     6901 aataatgatg aacttgttaa aagttactgt ggaaaaccta atctaatact ccatactgct
     6961 gaaccaggaa gccctttttg tgttattgat aaaataaaac ctaaaccaac aaaacaggaa
     7021 ataaaaatct ctgctataat ctgtgcatct aaaagccagg attggagaga caacaaagga
     7081 gatgttaaag ttcatcaatt cacaggaaaa gaagtttata agaaaaaatt tatgaaaagt
     7141 gggacatggg gaataaataa aaaaccaaag ataattaaag taaaaaagaa agagatagaa
     7201 gaatatattg aaaaaaataa aatcaaggaa taaaaatgcc ggcaataaga caaatacaat
     7261 taggaaaaaa cggggttaca gaacaattca ttagtaatct taaacaccat tttaataata
     7321 ctaaaaatgt taaagtttct gtcttgaaaa gttgttgtag agacagagaa gaattaagag
     7381 agataaatga gaaaatacta agagagctgg gaaacaatta tacatcaaga ataattggat
     7441 atactattgt gattaagaaa tggagaagag ataggagaga gaacttaaaa gaaaatcccg
     7501 aagaagaaaa ggaagtataa tcaaaccaaa taatttataa accctcctta accataattt
     7561 ctaggtaaat aactctaaga gttaatctat tgtcgtgaga caagccgaat aaattagaat
     7621 aaaactaaaa tggaaaatcc agtatatgat atgcgtgctc aagagtacaa tataaaatta
     7681 gctgaagctt taaaaaaaat acctgaattt aatgaaccag aatgggcaaa atttgttaaa
     7741 tctggagcag gaaaagaaag accaatagat gatgaagatt tctggcataa aagagctgca
     7801 tctgttctta gatgtattta caaaaaaggt tctattggag ttcaaagatt aagaacaaaa
     7861 tacggttcaa aaaaggacag agggtttaaa cctgaaaaat tttctaaagg ttcaggtaaa
     7921 ataataagaa caattttaca gcaatcagat aaagctggat ttacagagat tcaaaaacca
     7981 attagaggtg tgagaagcaa aaaaccagga agaatattaa cagataaagg gaaaaaattc
     8041 ttagagggga ttggaggagg aaaatgatag agtattctct aatgaaaaac aataagccat
     8101 taaccattga tgaattaatg gaagttagtg gtgttgttgg agcattattt caagaaacag
     8161 aaacccataa gatggaaaga actgtaagaa aaacttatgg agttaatgga caggcatttc
     8221 atatttatta taatgagaga gctagcaaaa tatttcttcc agaaaaatat gaaaaacaaa
     8281 ttaggaatat gttgtctgaa aaaggaggat taaaatgcct ataaaaaaca aaaataaaaa
     8341 aagaattctt gcaaaaaaag gaaggcaaac aaagtgggca ccagtttggg ctgtacttaa
     8401 aaaatacgga acaggaaaga gagtacatcc ttcaattatg acaaaataca agcgctcatg
     8461 gagaagaact aaacttcacg ttaaaccagg aaaaaggagg aaatcgcatt ttggttaaaa
     8521 tgacattaga agaaaatcct agatataaaa aatattgtgt atactcgaat atgaaatctg
     8581 aaattactgg aatggatttg cctaaatcag atgaaccaga ccacaaaata ttcataatta
     8641 atggaaagta tgattttaat ggtttagatt taagttctta taattggaaa aaatcaaata
     8701 caattactcc tcaacaaaag aaatttcttg aaacaacaac agggtataaa gattacaaag
     8761 aaaatgtttt ttatcaaggg cctcaaggaa atgtaatgat gcatttaaat tataaaacag
     8821 atattcataa attgaaagtt aatctgaata aagcatcaag agatactatg aaagcagtaa
     8881 aatctatgtt tgaattagaa ttggggtgta tatgttatga ggaagataat cttccagaag
     8941 gattgttaga aaaaattcgg gacatagaaa gggctaaagc aaaattaaat taaaatggca
     9001 aacaaaaaat cagaaaacaa gattgaaaga gaatatgtta ttcctttaag agagaaaagt
     9061 agagttgttg caagatacaa aaaaactcca aaggcaataa agacaataaa ggaattttta
     9121 gctaagcata tgaaagtaga aaacagagat ttgaataaaa ttaaaattga caaagattta
     9181 aatcaattct tatgggctag aggaataaga aaacatcctc ataagataaa agttaaagca
     9241 ataaaaaaag gagaaaatat aattgtgcag cttgtagatt atccagataa tcttaaattc
     9301 aaaaaattaa gagaagaaaa ggcaagaaaa gctgctcttg agaatataga aagtaagaag
     9361 ggcatgatgc aaaagatgaa agagagcttg caaaaaccag aagaaaaaga cgaaaataaa
     9421 gacggtgtag atgataaaaa agaaattgct gaaaaagaga aagctactgt agaagcaaac
     9481 cagaaattcc agaaagaaaa agccaagcaa gcaaaacatc agactgaaaa accaaaaaaa
     9541 gcagttaaac ctgtaagagt agcacttcag aaataaatta taaattttct tgataaaaat
     9601 attgtaaatt aattatctta ataaaccata tatattaagt tattatacaa agtataaaga
     9661 ggattaaaga tgctgaatag attaaaaaaa gaaattgaag aagctaaaaa tcctgaacaa
     9721 gctaaaatac tccaaagatt ttttaaaaca ggtaaaggag agtacggtga aggagacatt
     9781 ttcttaggga taaaagtacc tatacaaaga aaaattgcaa agggatatac gaatttatct
     9841 tttatggatt tacagactct tttaaatagc aagatacatg aagaaagatt aattgcattg
     9901 ataatcctca caaataaata tcaaaaaagt aaaaaagatt ttgtaaaaaa aagacaaata
     9961 tttgaatttt atctaaaaaa cacacacaat ataaataatt gggacttagt ggatttaagt
    10021 gctccaaata ttgttggaga tttttcaagc acagatggaa cagaagtaat aagatttctt
    10081 gcaaaatcca aaaatctttg ggaaagaaga atagcaattg tttcaactta tgcattcata
    10141 aaaaagagaa tttttggaga gactcttgct attgcagata tgcttcttaa agatgaacat
    10201 gatttaattc acaaagcagt gggatggatg ctaagagaag ttggtaaaag aaatcaagaa
    10261 gttttagaga tatttctcaa agaaagatac aaaactatgc caagaacaat gctcagatat
    10321 tctattgaaa aatttcctga agaaaaaaga aaggattatc taaaaggaga aatataaatt
    10381 taattaatta ttttacattt tattttaata agatatacta taaaatatat attgcaatat
    10441 atactttgga taaatagatt aatcttttaa atcatagaaa attaagagtt ttatgaatat
    10501 agaagagata aaaaataaaa aaacaattaa ggaactgctt gaatttggga taataaattt
    10561 agataaacct tctggtccta caagttttag tgtttctgca tttacagcta aaagattgaa
    10621 attaagaaaa acatctcatt ttggaacact tgacccaaag gtaactggag ttttacctgt
    10681 tgctctcaat agagcatgta aattaacagg atatttttta ggtgaagata aggaatatat
    10741 tggagttatg agaatccatg aagatattga tattgaaata ataaaaaaag caatacaaga
    10801 gaagtttatt ggaaaaataa tacaaatgcc tcctgtaaag tccagagtaa aaagacagaa
    10861 tagaccaaga gaaatatact cttttgaatt attgcaaaag aaaggcagag atattttgtt
    10921 tagagttaaa tgcgaaggtg gaacatatat aagaaaatta atagatgatt tgggtaaaga
    10981 gacaaaaatt ggggcgcata tgcttgagtt aaggagatta agtgcaggta tctttgatga
    11041 aacctattct gtaaatatgt atgaatttga aaaagctgtt gaagagtatg aaaaaggaaa
    11101 tgagaaattt ttaagagaga taattatccc tgcagagatt gttggaaggg tctatcctgt
    11161 tatgaatata agagaagata atttaaaaca aatattaaca ggaaaaccta tttacaaaaa
    11221 ggatttaatt gaagatgaag ggtggaaagt tgattcaatt gtaagctgtt tttgtaatgg
    11281 taattttgtc gggatgtata aaatcttcag gggaaaagaa atatttgcta aacctgaatt
    11341 tgtaatgcag gaaatcagag gataaaatgc gtcaatttgt atatttttca agttcagctg
    11401 caacatctgg aagtgcttta tctaaatatt ctcaatatga tggggactta atgaaagcag
    11461 ggagaatgga tattgcaata cacagcttta ttcaaggagt ttttttatca catgatttca
    11521 gaaaagattg caaattccat tttgtctttt atggtatgcc tgaccctcca aaacatattg
    11581 aaatccaagt taaagatgaa acacaaatat ctaaaaaaga tgttggaaat atgataaaaa
    11641 aaatacttta caaatacaaa gaaaaagaaa aaacagaggt ttttccagga tgttttgttg
    11701 agaaaaaaag ttttcttagt gttattgaag aattaattgg gcaagggaat gaaattttta
    11761 tattggataa aaaaggagaa gatataagaa agataaatat ccctaaaaat tgtgttttta
    11821 taattggaga ccatgaaggg cttccaaaaa aagaacttaa aagattaaaa aattattgta
    11881 aacctgtttc tatagggaat aagatgtatt ttgcatcaca aacagttgct gttgtgaaca
    11941 atgaactaga ttatagaaat gtctaattat caaaatgttt aataatctct ttaaatataa
    12001 tagaatatag gagataatat gaaaaaagct gtgagaatgt atattgatgg aacaattcag
    12061 gatgtctttt ttagaggttt tgttaaagag aatgcagaaa gacataatgt aaaaggattt
    12121 gtaagaaatc ttgaagatgg aagagttgaa atatttttag aaggaaatat agacgatgtt
    12181 aataaagttg ttgaattatg taaaaaaggt ccaaaacact ctcaaataaa agggatagat
    12241 atgcgtcctg aaaaatttca ggatttcaag acattcaaga tattacacat ttaatcttca
    12301 aaaaatatac ccatacgatt tctgtggaaa acatcaagtc catgccaatt tttatttaaa
    12361 gttgaatagt ttatacttct tgtttttccg taaagagggt cataatagaa aactttttcg
    12421 tgtttagtgt cataaccatt tgcaacaaca tagtgtccgc ttttccattc ctgaataagt
    12481 aagattacag gatttttagt gtttattgaa taaataatgt catctattga agcgttatgt
    12541 tttagtttgt gatttaggtt aaaattttta gatatctgaa tcattcctct tactggagtt
    12601 cctgtttcag tagaacaaca ggacatatta atcaattgat attctggaat atctaaccct
    12661 attttataca atataacttg cttcattgca tttggtccgc atgagctaag atttatttgt
    12721 ttgtttcctt ctgggaattt caatattttt ttcatttcat ataaaaataa aaatctaaga
    12781 atgttttata aacgttgtca ttataattaa tacaaatgat atatacaaat tcagatggag
    12841 gtgctagagg aaatcctggt cctggagcag ttgctgctct tgtaagaaaa gaaggggaaa
    12901 ttataactaa atacagtaag tttataggtg aaaacacaac aaacaatgtg gcagagtatg
    12961 aagggttaat tcttgcattg gaattagctt caaatataac taaagatgaa ataacttgtt
    13021 ttttagattc agaacttgtt gttaaacaac ttttaggaga gtataaagtg aggaataaaa
    13081 atattcttcc attatttctt aaagttcaaa aactccaaga aagatttaag aaaataaaat
    13141 atgcacatgt tccaagaaca gataattttc aaataatagt tgatgaaatg ttaaatgaag
    13201 aattagataa taaaggatat agaaaaaata gattcagaag aagaaaatga aagtaaagat
    13261 taaatataaa aactaaaatt atttttaatg gcagatttta aaattccgca attttctacc
    13321 aaagatgtct t
//
LOCUS       DAOPCH010000020        12396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_5057_length_12396_cov_4.961024, whole genome shotgun sequence.
ACCESSION   DAOPCH010000020 DAOPCH010000000
VERSION     DAOPCH010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 12396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12396
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5057_length_12396_cov_4.961024"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..561
                     /locus_tag="PLU55_02345"
     CDS             49..561
                     /locus_tag="PLU55_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86930.1"
                     /translation="MIKKSADKNKSRIKKVSKEKKNKELEKVLIDNFVSLQKVMVNLS
                     VKFDDLTNQISKLLELFETSAKVLAEKDFDVEKNNKENAKILERLETVLEQNRTIARG
                     LTLMHDKMNEPAPTPPTNQGYFPSQLNQRYPQQQPQAPQPSMFKPIQKTSMIGGVTEE
                     YHKPISSNGH"
     gene            551..1381
                     /locus_tag="PLU55_02350"
     CDS             551..1381
                     /locus_tag="PLU55_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86931.1"
                     /translation="MDTKIKKLKEEPSFIEFKKAFLLEFTRQLIIHSTHADILKLETI
                     LEKEEVVTPKPLTKKEIIRDKIRRREEIQEERMEGTRSILRPAIGMFDTHVIPEISPF
                     RDTFKKEKIKEKKQIEVTNKPLYRNEPRPEVSQIIVKNIPYQDPFRKIELWMPDVNLP
                     PHIQYLKPVPINKSIDLGKLNPLINDPMVKRIECYGANENIGVKGGMGAKKTAIVLTK
                     EEIEDIINRFSKETKIPVQEGIYKVVSGRLVLLAVISSVAGIKFTINKMSFEEMNQNV
                     "
     gene            complement(1369..2802)
                     /locus_tag="PLU55_02355"
     CDS             complement(1369..2802)
                     /locus_tag="PLU55_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86932.1"
                     /translation="MAYKKYIKRNGKLYGPYIYESKRVNGKVVSEYHGSEEPKKEKGV
                     KINQFNYKKIFLFFIGAILLAVLIYFFIFNINKNSFSGNVIASINSFYEQGEILDGLL
                     EFSLNEGELIPASSKIIFENSNISQEFNLRDFLDSHISEGKYYVKDVELEGEGEGYGL
                     EGERVVYPELSFTLKIYQTIDDDSEENTEVLEETPTEENISTPITGNSIKSSGRFLSS
                     FFRTTGSVISMELKQDIQGKVSKDNEFVYELNEGEEAELELKSVYYGEENLPDKTIEL
                     KKEDNKIIITTEYSFVEKGYGEDYLGSKNKKLTLNLEDFNLSVEEGNLIVKVIYEDDE
                     IFSLNTVIKEGEKVSDELIEIPLEEEKEPILNEEVEIKQEEPILIRDIGKSLTSKERE
                     VILNKFGESSIQVINSEKFKDRIIINYALGEYYIEYSYDSDLDKNTLDSLMEEDRIKF
                     LKDIAKSLVQEENLAEPINDFKDNYTF"
     gene            complement(2865..3572)
                     /locus_tag="PLU55_02360"
     CDS             complement(2865..3572)
                     /locus_tag="PLU55_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86933.1"
                     /translation="MDKSKKRLFFGVAFVVLFLMISGVFAESLGLTSGTKEAIDKVIG
                     NNGIDKDKVKSIDKVDFEALPDQVNLKDIDTTNLAVYEVDYGGESPIFVITASDELFA
                     SKETEKVYKKMLLNFGKNGEVETSTFLNSATGVEGNYDNGYVMMREGSITGISTSLEA
                     VSGKGDVEIIININGEPIGFRNTISVDSAGFKKDYDTQSLGVVKFNKGDIISVSVKLD
                     GGVIVKNIINLIEISTE"
     gene            3625..3906
                     /locus_tag="PLU55_02365"
     CDS             3625..3906
                     /locus_tag="PLU55_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF357 domain-containing protein"
                     /protein_id="HPJ86934.1"
                     /translation="MKDKITLEKINKYFEITEKALKEVKKNIIKGKENYAKEIIDMVE
                     NYLSDAKHFKNKEDYVNCFAALNYAHGWIDSGVRMDVFDVKDDKLFTVK"
     gene            3954..4643
                     /gene="pyrH"
                     /locus_tag="PLU55_02370"
     CDS             3954..4643
                     /gene="pyrH"
                     /locus_tag="PLU55_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPJ86935.1"
                     /translation="MEKKVIVLSLGGSLIIPDEINTKYLKSFKKTILKNTSNYKFIVV
                     CGGGNIARKYISALKKVGMNTLFQSYAGISATRTNARFMSYFFNQDQKRGIPKKIREV
                     KKYIKKYDIVFCGALEYRPNQTSDSTASEIAKKFKCEFINLTDVNGLYDKNPKEFKNA
                     KLIHEISWEEFKKIVDKIKFRPGQHFVLDQTASKTIKTNKINTYILGKDMRQLDNLLN
                     GKKFIGTKISG"
     gene            4644..5111
                     /locus_tag="PLU55_02375"
     CDS             4644..5111
                     /locus_tag="PLU55_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P subunit p30 family protein"
                     /protein_id="HPJ86936.1"
                     /translation="MKDTQILITQKDFNKLKNEVKKAKEQGKQIIYFSEDDELNRKVI
                     EKLEISVLLLTLNQRKDFQKQRNSGFNQVMARTMNKKGIYLGINLDEIIEETNYKKKS
                     EIFARIRQNIEICNKNKVAMKFIALKEKNKRNVYDLKSLGLVLGMPTWMTKQF"
     gene            5144..6658
                     /locus_tag="PLU55_02380"
     CDS             5144..6658
                     /locus_tag="PLU55_02380"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF003210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPJ86937.1"
                     /translation="MDKTKIIESLSPNEKKILPYLGEKISEICKKSNLDKTSVIRSLE
                     YLQNKEIVKINYIKNKIIELDVNGALYLKKGLPERRLLNLLDEKHIVSLQDAQKEGNL
                     SEEEFKASIGALKKKALIDLKNGKIILNASKSEISKKTLEEQFLENLPTDYETLKPEY
                     LLALKNLQNRKQIIGIKEDKKINIEITPLGKEILKSKISTQELIEQITPEILKKESNW
                     KGKRFRRYDVTSPVPEINGGKRHFVNQSIDYARKIWTEMGFQEMKGDIIQSSFWTFDA
                     LFTAQDHPVREMHDTFFINKKVELEDRKIVSKVKEAHEKGISGSKGWQYSWDEEEAKK
                     LVLRTHTTCLSSRTLANLKKEELPAKFFAIGKCFRNETVDWAHGFEFYQTEGIVIDKD
                     ANFRHLLGYLKQFYKKMGFENVRFRPGYFPYTEPSVEVEVFHPEKKKWIELGGAGMFR
                     PEITVPLLGEHVPVLAWGQGFDRIIMDYYNIKDLRELYKNNFTQLRKMKFWMKQ"
     gene            6660..7127
                     /locus_tag="PLU55_02385"
     CDS             6660..7127
                     /locus_tag="PLU55_02385"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPJ86938.1"
                     /translation="MEKTDNKKNSDCIFCKIASGEIPSVKIWEDEKHLAILDINPNTE
                     GMTLVITKNHFNSYIFDLPDKNYVELLNASKEVAKILEKGLNVERVAMVFEGQGVNHI
                     HTKLYPMHNINTAKFGDLTGNVYFKEYPGYITTLVGPKRSPEELKKVADKIKK"
     gene            7129..8778
                     /gene="pheT"
                     /locus_tag="PLU55_02390"
     CDS             7129..8778
                     /gene="pheT"
                     /locus_tag="PLU55_02390"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPJ86939.1"
                     /translation="MANITFSRKIFEKEIGKLDEKMQEKISMFGTPLETFDDETIEIE
                     VFANRPDLLSYQGFKRCFLAFLGKKPGLRKYETNKPEKDYQVIIDSSTKDVRPYTACA
                     IVKGLKLDEEKIKEIIGIQEKLHLTMGRKRKKVAIGIYPLEKIKLPITLKALEPDYIK
                     FIPLESEKEMSGLQILQKHPAGKEYSHLLAGKSKFPVFVDANNNILSMPPIINSQMTG
                     KVTTETKDVFIECSGFDFKTLKKCLNILVIVFADMGGKIYQMELKGAPEKISPVLESE
                     KMKISLENTNKTLGINIDEKKIKTLLERMGHNYNPKTKEVEIGAWRSDILHEVDLIED
                     VAIGYGYGNLNPEIPKISTIGKENSEEIIKRKISEILTGLGLLEVSNYHLTNKESRFE
                     NMNVSEKQEKDIIYLENSKTEYNLLRKDISSFLLKNLSENVDSEYPQKIFEIGTVFSL
                     KQNIQEKENLAIAITPGNFTEIKQILNYLAKSLDLKIDFKENDSPSNYLIEGRTTNIL
                     FENKEIGIMGEVHPKILKNWKIKMPVAILEINLEDIIKKLS"
     gene            complement(8775..9272)
                     /locus_tag="PLU55_02395"
     CDS             complement(8775..9272)
                     /locus_tag="PLU55_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86940.1"
                     /translation="MTKEKKEKSKLQKLKEKYKDLKKEYNLPEFDELNKEFSIEKISD
                     VETDFLMREIGRVMSDKFSSYLRITEMMLNPINSPIFVFSMVKAMGEDEKKKLSEAYK
                     ELTKIELNLIELDVNFSMKKQAEFIINSYKSWKEIKGYFSEVISKVKSNWDSKHEDNN
                     KAYFG"
     gene            9418..9490
                     /locus_tag="PLU55_02400"
     tRNA            9418..9490
                     /locus_tag="PLU55_02400"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9451..9453,aa:Thr,seq:ggt)
     gene            complement(9579..9875)
                     /locus_tag="PLU55_02405"
     CDS             complement(9579..9875)
                     /locus_tag="PLU55_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86941.1"
                     /translation="MVKKKRVVKKKTNKKPKIVSKIEKNKVFSTKVGLGVVLKNLSIF
                     IFLFLISLALYATAIPKESGFSILFGILAIISFLISMAFFLALLALLVLKWMKK"
     gene            complement(9918..10247)
                     /locus_tag="PLU55_02410"
     CDS             complement(9918..10247)
                     /locus_tag="PLU55_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86942.1"
                     /translation="MVNVILVLVIALIAIIWLSQEFKEVKNKFFTVFLILLLVFTCLS
                     FSYAIKGRDIDLKTTDGLKEAGHIYVLWFGQAFRNIKVVTGNAIGMNWKLDENVSVNE
                     SVKKPKK"
     gene            complement(10304..10376)
                     /locus_tag="PLU55_02415"
     tRNA            complement(10304..10376)
                     /locus_tag="PLU55_02415"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10340..10342),aa:Gln,seq:ctg)
     gene            10492..11094
                     /locus_tag="PLU55_02420"
     CDS             10492..11094
                     /locus_tag="PLU55_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HPJ86943.1"
                     /translation="MKAKKGKTSNKNKKLEEVLIQVGLRIAKRGEGALFVVGETKYTP
                     LIDQSVPPFKASNNPKLLESLALMDGAVIINKKGFVIAYGVRIKTTKIFKNFGTRHSA
                     AISAATGDNIVVIVSEEDKKVRVMKKGKTVMQIDALQKNVEKDVSKAVKVLETIGAGT
                     VGAVGTSILFPPLGVAILPGIVIFGSAYYLGKLIQKKIGK"
     gene            complement(11465..12103)
                     /locus_tag="PLU55_02425"
     CDS             complement(11465..12103)
                     /locus_tag="PLU55_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86944.1"
                     /translation="MTLKLSKTKDEFIDRIFEECMNELKEFFKINWTRNRPSIIIFED
                     RETLDELKGSKTDDWNVGFATNNKIYLLDRRNYEKESCHKYSDEEYKKLLKHELVHLH
                     YDIFSKGAHYPRWLNEGLAVFLSEQLENKNLEKFEKFLDYFEKRGDSLYTEGGFAIKI
                     LIENFGQDKLFELIKNISSLQTEEKFNSKFQEIYGEKPSYEFFNSLIKNKES"
BASE COUNT         4939 a         1655 c         1747 g         4055 t
ORIGIN      
        1 aattactcaa ttataaatat ttataaatat ttaattcgtg tataaactat gattaaaaag
       61 agtgcggata aaaataaaag tagaattaaa aaagtttcca aagaaaagaa aaataaagaa
      121 ttggaaaagg tccttataga taattttgta tcgttacaaa aagtaatggt taatctttca
      181 gtaaaatttg atgatttgac taatcaaata tctaaattat tagaattatt tgaaacatct
      241 gccaaagttc ttgctgaaaa agattttgat gttgaaaaaa ataataagga aaatgcaaaa
      301 atactggaga gattagaaac agttttagaa caaaatagaa ctattgcaag aggattaact
      361 ttgatgcatg ataaaatgaa tgaacctgct ccaactcctc ctacaaatca aggatatttt
      421 ccttcacaat taaatcaaag atatcctcaa caacaaccac aagcccctca accttcaatg
      481 tttaaaccaa ttcaaaaaac ttctatgata ggaggggtca ctgaagaata tcataaacct
      541 atctcctcaa atggacacta agataaaaaa actaaaagaa gaaccgagtt ttatagaatt
      601 taaaaaagca tttttacttg agtttacaag acaattaata attcattcta cacatgcaga
      661 tattttgaaa ttagaaacta tcttagaaaa agaagaagtt gtgacgccta agcccctaac
      721 taaaaaagaa ataatacgtg ataaaataag gaggagagaa gaaatacaag aagagagaat
      781 ggaaggaaca cgttcaatat tgcgtcctgc aataggaatg tttgatactc atgtaatccc
      841 cgaaataagt ccttttagag atacttttaa aaaagaaaag ataaaagaaa aaaagcaaat
      901 agaagttact aataaacctc tatatagaaa tgaacctcgc ccagaagttt ctcaaataat
      961 agtaaaaaat atcccttacc aagacccgtt taggaaaata gaattatgga tgccagatgt
     1021 aaatcttcct ccacatattc agtatttaaa accagtacca ataaacaaga gtattgattt
     1081 agggaaattg aatcctttga ttaatgaccc tatggtaaaa agaatagaat gttatggtgc
     1141 aaatgaaaac ataggagtta aaggaggtat gggagctaag aagacagcaa tagttttaac
     1201 aaaagaagag attgaagata taataaatag attttccaaa gaaacaaaaa tacctgttca
     1261 agaaggaata tataaagtag tttcaggaag acttgttctt ctagctgtga tatctagtgt
     1321 tgcaggaata aaatttacaa taaataaaat gagttttgaa gaaatgaatc agaatgtata
     1381 attatcctta aaatcattta taggttctgc taaattctcc tcttgaacaa gagattttgc
     1441 tatatcttta agaaacttta ttctgtcttc ttccattaaa gaatctaaag tatttttatc
     1501 taaatccgaa tcataagaat attctatata atattctcca agagcataat tgataattat
     1561 tctatctttg aatttttctg aattaataac ttgtatagaa ctctctccga atttgtttaa
     1621 tatgacttct ctttctttgg aagttaaaga tttaccaata tctctaatta aaatgggttc
     1681 ttcttgtttg atttcaactt cttcatttag tattggttct ttttcctctt ctaatggaat
     1741 ttctattagc tcatctgaga ccttttcccc ttctttaata acagtgttta atgaaaatat
     1801 ctcatcatct tcgtaaataa ctttgacaat taaatttcct tcttcaacag ataaattaaa
     1861 atcttctaaa tttaaagtca attttttatt tttagaacct aaataatctt ccccataacc
     1921 tttttcaaca aaagaatatt ctgtggttat aattatttta ttatcttctt tctttaattc
     1981 aattgtttta tcaggaagat tttcttctcc ataataaaca cttttcaatt ctaattcagc
     2041 ttcttctcct tcatttaatt catagacaaa ttcgttgtct ttagatactt taccttgaat
     2101 atcttgctta agctccatag aaataacact tccagttgtt ctgaaaaaag aacttaaaaa
     2161 tctcccagaa gattttattg aattacctgt tataggagta gatatgtttt cttcagtagg
     2221 ggtttcttct aaaacttctg tattctcttc tgaatcatca tctattgtct ggtatatttt
     2281 gagagtaaaa gataactcag gataaactac tctctctcct tctaatccat atccttctcc
     2341 ttctccttct aattcaacat ctttgacata atattttcct tcagaaatat ggctatctaa
     2401 aaaatctctt aaattgaact cttggctaat atttgaattt tcaaaaataa tttttgaaga
     2461 agctggaatt aactctcctt catttaaaga aaattctaaa agaccgtcca atatttcccc
     2521 ttgttcatag aaactattta tactagcaat aacattacct gaaaaactat ttttatttat
     2581 attaaaaata aaaaaataaa ttaaaacagc aagtaatatt gctccaataa aaaacaagaa
     2641 tattttttta taattaaatt gatttatttt aacacctttt tcttttttag gttcttcact
     2701 tccgtggtat tctgaaacga ctttgccatt aactctttta ctctcgtaaa tataaggacc
     2761 gtaaagtttg ccgttccttt taatatattt tttatacgcc atcttttata atcaaataaa
     2821 agaattatct tatttaaatt ttgttagaaa ataaaaatat aattttattc tgttgaaatt
     2881 tctatcaaat taataatatt ttttacaata actcccccat ctaatttaac agaaacagag
     2941 ataatatctc ctttgttaaa tttaacaact cctaaagatt gtgtatcata atctttcttg
     3001 aatccggctg aatcaacaga tattgtgttt ctaaatccta taggttcccc atttatatta
     3061 ataattatct ctacatctcc ttttccacta acagcttcta aacttgttga aattcctgtt
     3121 atacttcctt ccctcatcat aacataacca ttgtcataat ttccttcaac tcctgttgcg
     3181 ctgttaagga aagttgaggt ttctacctct ccatttttac caaaattcaa aagcattttt
     3241 ttgtaaactt tctcagtttc ttttgaagca aacaattcat cagaagcagt tataacaaaa
     3301 ataggactct ctccaccgta atctacttca tatactgcta aattagttgt gtcaatatcc
     3361 tttaggttaa cttgatcagg aagtgcttca aaatctacct tatcaattga tttaactttg
     3421 tctttatcta taccattatt accaataact ttatctattg cttctttggt tccacttgtt
     3481 aaacctaatg attctgcaaa aacccctgat atcattaaga ataatactac aaaagctact
     3541 ccgaaaaaca acctcttttt actcttgtcc attaaaaaat aagcaaaaag atatatttaa
     3601 agattatctt aatttaatta taagatgaaa gataagataa ctcttgaaaa aataaataaa
     3661 tattttgaaa taacagaaaa ggcacttaaa gaagttaaga aaaatataat aaaaggaaag
     3721 gaaaattatg caaaagagat aatagatatg gttgaaaatt acctctcaga cgcaaaacat
     3781 ttcaaaaata aagaagatta tgttaattgt tttgctgcat taaattatgc tcatggatgg
     3841 atagattctg gggtgaggat ggatgtattt gatgttaagg atgataagct ttttactgtt
     3901 aagtaaattt aaatttagca agatataaaa actaaagtta aattataata aaaatggaaa
     3961 aaaaggtgat tgttttaagt ctaggaggga gtttaataat acctgatgaa ataaatacta
     4021 aatatcttaa aagcttcaaa aaaacaattc taaaaaatac aagcaattac aaattcatcg
     4081 ttgtatgtgg agggggaaat atagcaagaa aatacataag tgctttaaaa aaagttggaa
     4141 tgaatacttt attccaaagt tatgctggaa tctcagctac aagaacgaat gcaagattta
     4201 tgagctattt tttcaatcaa gaccaaaaaa gaggaatacc caaaaaaata agggaagtta
     4261 aaaaatacat aaaaaaatat gatattgttt tttgtggtgc ccttgaatat cgtcctaatc
     4321 aaacttcaga ttcaacagca tctgaaatag caaaaaaatt taagtgcgaa ttcataaatt
     4381 taacagatgt taatggtttg tatgataaaa acccaaaaga gttcaaaaat gctaaattaa
     4441 tccatgaaat atcttgggaa gaatttaaga aaatagtaga taagataaaa ttccgtccag
     4501 gacaacattt tgtacttgac caaacagctt caaaaacaat aaaaaccaat aaaataaaca
     4561 catatattct tggaaaagat atgagacaat tagataatct ccttaatgga aagaaattta
     4621 taggtacaaa gatttctgga taaatgaaag atacacagat actcataaca caaaaagatt
     4681 tcaataaact taaaaatgaa gtgaaaaaag caaaggaaca aggaaaacaa ataatttatt
     4741 tttcagaaga tgatgaacta aataggaaag ttattgaaaa actggagata agtgttcttc
     4801 ttttaacatt aaatcaaaga aaagattttc aaaaacaaag aaattcagga tttaatcaag
     4861 ttatggcaag aacaatgaat aagaagggga tatatttggg aataaattta gatgaaatta
     4921 tagaagaaac aaattacaaa aagaaatcag agatatttgc aagaataaga caaaacatag
     4981 aaatatgcaa taaaaacaaa gtggctatga aatttattgc attaaaagaa aaaaacaaaa
     5041 gaaatgtcta tgatttgaaa tctttagggt tagttcttgg aatgcctact tggatgacaa
     5101 aacaatttta aataggtctc atctaatatt aaaatatatt aaaatggata aaactaaaat
     5161 tattgaatct ttaagcccta acgaaaagaa aattttgcct tacttgggag aaaagataag
     5221 tgagatatgt aaaaaatcaa atttagataa aacatcagtt attaggtctt tagaatattt
     5281 acagaataaa gaaatagtaa aaataaatta tattaaaaac aaaataatag aattagatgt
     5341 taatggagca ttatatttga aaaaaggctt acctgaaaga agattattaa atttattaga
     5401 tgaaaaacat atagtttctt tacaagatgc acaaaaagaa ggtaatcttt cagaagaaga
     5461 gttcaaggca tctattgggg ctctaaagaa aaaagcattg attgatttaa aaaatggaaa
     5521 aataatatta aatgcaagta aatctgaaat atcaaaaaag acactcgaag aacagttttt
     5581 ggaaaatctt ccaacagatt atgaaactct aaagcctgag tatctgcttg cattaaaaaa
     5641 cctccaaaat agaaaacaaa taatagggat aaaagaagat aaaaaaataa acattgaaat
     5701 aacaccatta ggaaaagaaa tacttaaatc taaaataagc acacaggaat tgatagaaca
     5761 aataactcca gaaatattaa aaaaagagtc taattggaaa ggaaaacgat ttaggagata
     5821 tgatgttact tctcctgtgc ctgagataaa tggtggtaaa agacactttg taaatcaatc
     5881 aatagattat gcaagaaaaa tatggacaga gatgggattt caggaaatga aaggagatat
     5941 tatccagagt tcattctgga catttgacgc tttatttact gcacaggacc atccagtaag
     6001 agagatgcac gatacttttt ttataaacaa gaaagttgaa cttgaagaca gaaaaatagt
     6061 ttcaaaagta aaagaagctc atgaaaaagg aatttctgga agcaaaggat ggcaatattc
     6121 ttgggatgaa gaagaggcaa agaaattagt tctaagaacc catactacat gtttatcttc
     6181 aagaacatta gcaaatctga aaaaagaaga attacctgca aaattttttg caataggaaa
     6241 atgctttaga aatgaaacag ttgattgggc tcatggattt gagttctatc aaacagaagg
     6301 aatagttata gataaagatg caaatttcag acatctttta ggatatttaa aacaattcta
     6361 caaaaaaatg ggatttgaaa atgtaagatt cagaccagga tatttccctt acactgaacc
     6421 ttctgttgaa gttgaagttt ttcatcctga aaaaaagaaa tggattgaac ttggaggagc
     6481 aggaatgttc cgtcctgaaa taactgttcc tttattagga gaacatgtac ctgttttagc
     6541 ttggggacaa ggatttgaca gaataataat ggattactat aatataaaag atttaagaga
     6601 attatacaaa aataatttca ctcaactaag aaaaatgaaa ttttggatga aacagtaaaa
     6661 tggaaaaaac agataataaa aaaaacagtg attgtatatt ctgtaagata gcaagtggag
     6721 aaataccttc tgtaaaaatt tgggaggatg aaaaacattt ggcaatatta gatataaatc
     6781 caaatactga gggaatgaca ttagtaataa caaaaaatca ttttaattca tatatatttg
     6841 atttacctga taagaattat gtagaattat tgaatgcatc aaaagaagtt gcaaaaatcc
     6901 tagaaaaagg attaaatgta gaaagagttg caatggtatt tgaaggacag ggagtgaatc
     6961 atatacacac aaaattatac cctatgcata atattaatac tgcaaaattt ggagatttaa
     7021 ctggtaatgt ttactttaaa gaatatccgg gatatataac tacattagtt ggtccaaaaa
     7081 ggtctcctga agaattaaaa aaagttgcag acaaaataaa aaaataaaat ggcaaatata
     7141 acttttagta gaaaaatatt tgaaaaagaa atagggaaac ttgatgaaaa aatgcaggag
     7201 aagatttcaa tgtttggaac ccctttagaa acatttgacg atgaaacaat tgaaatagag
     7261 gtatttgcaa atcgtccaga tttactctct taccaaggat ttaaaagatg ttttctagct
     7321 ttcttaggta agaaacctgg attaagaaaa tatgaaacaa ataaacctga aaaagattat
     7381 caagtaatca ttgattcttc cacaaaagat gttcggcctt atactgcgtg tgctattgta
     7441 aaagggctta aattagatga agaaaaaata aaagaaatca taggaataca agaaaaatta
     7501 catctaacaa tggggagaaa aagaaaaaaa gtagcaatag gaatttatcc tcttgaaaaa
     7561 ataaaacttc caattacatt gaaagctctt gaaccagatt acatcaaatt tatacctttg
     7621 gaatcagaaa aagaaatgtc tggattgcag atattgcaaa aacatcctgc aggaaaagag
     7681 tattctcact tattagcagg gaaatctaaa tttcctgtat ttgtagatgc aaataataac
     7741 atcttaagca tgcctcctat aattaattca caaatgacag gaaaagtaac aacagaaaca
     7801 aaagatgtat ttattgaatg ttctggtttt gattttaaga ctttaaaaaa atgtttgaat
     7861 atacttgtaa ttgtatttgc agatatggga ggaaagattt accaaatgga attaaaagga
     7921 gctccagaaa aaatatctcc tgtattggaa tctgaaaaaa tgaaaatttc tcttgaaaat
     7981 acaaacaaaa ctctaggaat aaatatagat gaaaagaaaa taaaaacatt gcttgaaaga
     8041 atgggacata attataatcc taagacaaag gaagtcgaga ttggagcttg gagatcagat
     8101 atcttacatg aagtggactt gattgaagat gttgcaatag gatatggata tgggaactta
     8161 aatccagaaa taccaaaaat atctactata ggtaaagaaa attcagaaga gataataaaa
     8221 agaaaaatct ctgagatact tacaggatta ggtcttcttg aagtttcaaa ttatcatctc
     8281 acaaataaag aatctcggtt tgaaaatatg aatgtttcag aaaaacaaga aaaagatatt
     8341 atttatcttg agaattcaaa aacagaatat aatctattaa gaaaggatat ttcatcattt
     8401 ttattgaaaa atttatctga gaatgtagat tcagaatatc cccaaaaaat atttgagata
     8461 ggaacagtgt ttagtttaaa acaaaatatt caagagaagg aaaaccttgc tatagctata
     8521 actcctggta atttcactga aataaaacaa atattgaatt atttagctaa gtctttggat
     8581 ttaaagatag actttaagga aaatgattct ccttcaaatt atttaattga aggaagaaca
     8641 acaaatatac tttttgaaaa taaagaaata ggaattatgg gtgaagtgca tcctaaaata
     8701 ttaaaaaatt ggaaaataaa aatgcctgtt gcaatattgg aaataaattt agaagatata
     8761 attaaaaaat taagttagcc aaaataggct ttattgttgt cttcatgttt gctatcccaa
     8821 ttagatttta cctttgatat aacttcagaa aaataccctt ttatctcttt ccaagattta
     8881 taagagttaa taatgaattc tgcttgcttt ttcattgaaa aattcacatc aagttcaatc
     8941 aggttaagtt ctatctttgt aagttcttta tatgcttcag acaatttctt tttttcatct
     9001 tctcccattg ctttcaccat agaaaaaaca aatattggag aattaatagg gttaagcatc
     9061 atctctgtaa ttctcaaata acttgaaaat ttatctgaca ttactcttcc aatttctctc
     9121 attaagaaat ctgtttcaac atctgaaatt ttttctattg agaattcttt atttaattca
     9181 tcaaattcag gaagattata ctcttttttc aaatctttat atttttcctt gagtttttgc
     9241 aatttagatt tttctttttt ttctttcgtc ataagttttt attaataaaa aggttttata
     9301 aatatgttgg aattaataac ctcctgagta aaaatcaaat aatcaaataa gccgcagtag
     9361 ctcagtaggt aaagttctac gaacaacctc ctgagtaaaa atcaaataat caaataagcc
     9421 gcagtagctc agtaggtaga gcgttacctt ggtaaggtaa aggtcccgag ttcaatcctc
     9481 ggctgtggct taaaataaat ttgtctgtta tgaattgttt aaattgtaaa gttatacata
     9541 aaaatattat taagaaaaac taaggaaatt taatattttt attttttcat ccactttaat
     9601 actaataaag ctaataatgc taaaaagaat gccattgaaa ttaaaaagga aataattgct
     9661 aaaattccaa aaagaataga aaacccgctt tctttaggta tggcagtagc atataatgct
     9721 aaagatatta aaaaaagaaa gatgaaaatt gaaagattct ttaggactac tcctaaaccg
     9781 actttagtag agaatacttt atttttttct attttagaaa caattttagg ttttttattt
     9841 gttttctttt taaccactct cttttttttc accatattta ttataatgaa tgtttattta
     9901 taaatattac tctaacttta ttttttgggt ttttttacac tttcattaac tgagacattc
     9961 tcatctaact tccaattcat accaatagca tttcctgtta caaccttgat atttctaaat
    10021 gcttgaccaa accaaagaac ataaatatgg cctgcttctt ttaatccatc agttgttttt
    10081 aagtcaatat ctcttccttt tatagcataa gaaaaactaa ggcaggtgaa aacaaggagt
    10141 aagattaaaa aaactgtaaa aaatttattt ttaacttcct taaattcctg gcttaaccag
    10201 attatagcaa ttaatgcgat aactaagact agaataacat tcaccattaa tattacaata
    10261 taaaatacta taaaaagatt tcgaaataaa aaaataagaa aaatagcccc gccaggattc
    10321 gaacctgggt cacaaactcc agaggcttgt atacttgacc gctgtactac ggggcttttt
    10381 gtataataaa atgggtttca acaatttctt aaatcttact attttctcaa aatatatgtt
    10441 gtaaaatagc tataagaaaa atttatatat tgatatgaaa tacatattga catgaaagca
    10501 aaaaaaggaa aaacaagtaa taagaataaa aaattagaag aagtccttat acaagttgga
    10561 ttaagaatag caaaaagagg agaaggagca ttatttgttg ttggagaaac taaatatact
    10621 cctttaattg accaatctgt tcctcccttt aaagcatcaa ataatcctaa acttttagaa
    10681 tcattggcat taatggatgg ggctgtaata attaataaaa aaggttttgt tatagcatat
    10741 ggagtaagaa taaagaccac aaaaattttc aaaaattttg gtacaagaca ttctgctgca
    10801 ataagtgctg caacaggaga caatattgta gtaatagtat ctgaagaaga caaaaaagta
    10861 agagtaatga aaaaaggaaa gacagtaatg caaatagatg cccttcaaaa aaatgttgaa
    10921 aaagatgttt ctaaggctgt taaggttctt gagacaatag gtgcagggac agttggagca
    10981 gttggaacaa gcattctttt ccctccatta ggtgtagcta ttcttccagg gattgttatt
    11041 tttggctcag catattatct tggaaaactt atccaaaaaa agataggaaa ataaaaggga
    11101 atataaaata agttacccct agaaactaaa ttcttaattt gattttactt ttttttgatg
    11161 taataaacta aatattatat aaaacaagat aaattgaata aagaataata caaaattatt
    11221 agcagaaaaa aattctgaaa ggttatacat actgaagtat ctaaataaaa attttgggat
    11281 ttgattcata aggtctttga atgttacaaa tacaattacc cataataaag cagataaaac
    11341 cattgataat atcaccttta attttgttgg tctaaagttc atagataatg taaaataagt
    11401 gactttaaaa gattaccgag tagcgccttg ctggaagata tataatgaaa tgggatttag
    11461 gtatttagga ttctttattt ttaattaaag aattaaaaaa ttcataactt ggtttttctc
    11521 cataaatttc ctgaaatttt gaattaaatt tctcttcagt ttgtaaggaa gaaatattct
    11581 taattaattc aaataattta tcttgtccaa aattttcaat taagattttt attgcaaatc
    11641 ctccttctgt ataaagacta tcccctcttt tttcaaaata atctaaaaat ttttcgaatt
    11701 tttcgaggtt tttattctct aattgttcag ataaaaaaac tgctaatcct tcatttaacc
    11761 atctgggata atgtgctcct tttgaaaaaa tatcataatg taaatgaact agttcatgtt
    11821 ttaatagttt tttatattct tcatcagaat atttatgaca actttctttt tcataatttc
    11881 tcctatctaa aagataaatt ttattgttgg ttgcaaaccc cacattccaa tcatcagttt
    11941 tacttccttt taattcatct agagtctctc tgtcttcaaa gattattatt gaaggtctat
    12001 ttcttgtcca attaatttta aaaaattctt taagttcgtt catacactct tcaaaaatcc
    12061 tatctataaa ttcatctttt gttttagata gttttaaagt catttttata ttaattactt
    12121 gttttattta aaattattgt atgagtttat tcattgaatt tagaaatctt gaatgaattt
    12181 ttctttttta gctctttaag aacagaattg ttatatgaag tagtttttcc tgaaaaattt
    12241 tctgattttc caatcttagt tatcttggtt aaatagtatt tttttactct tcaaaattta
    12301 acctttataa ttgagcccta gaagtttggt tcttttttac atttaaaata atacggaaac
    12361 cacacttcat tttttaacgc cccgaccgag attcga
//
LOCUS       DAOPCH010000021        12194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_5159_length_12194_cov_3.918857, whole genome shotgun sequence.
ACCESSION   DAOPCH010000021 DAOPCH010000000
VERSION     DAOPCH010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 12194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12194
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5159_length_12194_cov_3.918857"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3005)
                     /locus_tag="PLU55_02430"
     CDS             complement(<1..3005)
                     /locus_tag="PLU55_02430"
                     /inference="COORDINATES: protein motif:HMM:NF007175.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HPJ86945.1"
                     /translation="MAKQGHIIVKNYLNEHSLVESNIISFNNFIEKKLQEIVNEISET
                     ITNEEFEITLGKIEVGKPRVTEADGSASLITPAEAQLRNLTYAAPITLELTVKKDGQI
                     DSEVVEIGRIPIMVKSKNCNTMGMSKEDLIANYNDPLDPGGYFIIKGNERVMIMAEDL
                     AENQPFIENNKKGDLTLKLFSLKGTYRIPITITENKEGIFNVSFSRFKDIPAIVLLKA
                     LGLTKESDIAKYIGKETDSVIVNLYEYTNMAEKEDAMMFIAEKTNLQGTKKEILDRVK
                     QRIDSYLFPHIGQKKEDRMKKAVTLCKFIKQFLVAKENPRLRTDKDHYANKRVRLSGD
                     LLATLFRVNLGILVRDIQYSLQKSSKRKKFFSIKVLAKSTLFSHRVESAIATGSWTGE
                     RSGITQNIDKTNYLATISQLQRVSSMLPGDQENFMARTLHPTHYGRFCPIETPEGTEI
                     GLRKNLAILARVSTRVELDESKFMKDLESLELNKEDIGNYDVFFNGMFIGTVNDGNKF
                     VKQIREKRRENQLPVQMSFREDEGIKTIYISTESGRVLRPLIIVENGTSKLKSEHLVQ
                     IEQGEIKWKDLIEKGIIEYLDAAEEENALVALNEESLTQEHTHLEIDPVSLFGTVTSL
                     VPYGNHDQSSRLNRGSKTQKQALGLYSANYLCRLDTDVSILQYPQKPIVRSFVYDTLN
                     TYPAGQNLVVAIMAYEGYNMEDALVLNKGSLDRGLGRSFYFRPYSAIEMNYAGGLKDE
                     IAMPEKDASGYRMEASYRHLEDDGVISPEAEVSEGEVMIGKMSPPKFLSEAREISIRT
                     KKESSVAMRQEEKGIIDSVFITEDNEGNKIVQVKTRDQRIPELGDKFATSHGQKGVIG
                     MIVPEEDVPFTSRGMRPDIIFNPHGLPSRMTVGYLLELLAGKVGSLRGEIVDGTSFSG
                     ASKKELEEQLEDLGFRFDGKETMYNGITGKKIVSRIFVGDLYYLKLKYMVGNKMHGRA
                     SGKVALLTRQPIEGRARGGALRLGEMEQQA"
     gene            complement(3013..3222)
                     /locus_tag="PLU55_02435"
     CDS             complement(3013..3222)
                     /locus_tag="PLU55_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit RpoH/Rpb5
                     C-terminal domain-containing protein"
                     /protein_id="HPJ86946.1"
                     /translation="MHILQPKHTKLNEKEVQELLKKYNISKSQLPKILLNDAALADMN
                     CAVGDIIKIERKEEDKINLYFRVVV"
     gene            complement(3490..3564)
                     /locus_tag="PLU55_02440"
     tRNA            complement(3490..3564)
                     /locus_tag="PLU55_02440"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3527..3529),aa:Lys,seq:ttt)
     gene            complement(3699..4010)
                     /gene="trxA"
                     /locus_tag="PLU55_02445"
     CDS             complement(3699..4010)
                     /gene="trxA"
                     /locus_tag="PLU55_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPJ86947.1"
                     /translation="MVAELTNGSFNEFIKEGTVLIDFYADWCMPCVMMGPIVEEVAEK
                     FNGKLKVGKVNVEDGHEISQKFGVSSIPNFVLFKDGKVITNFVGAMSEEEFSEKIQKF
                     L"
     gene            complement(4035..4514)
                     /locus_tag="PLU55_02450"
     CDS             complement(4035..4514)
                     /locus_tag="PLU55_02450"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPJ86948.1"
                     /translation="MNLTIIAAISKNNVIGKYGEIPWYIPEDIKRFKDITFNHPVIMG
                     RKTYESLSEKFKPLPGRKNIVLSNTLNEQSGIYLARNIEQAIGFTDNKDSFIIGGGEI
                     YKKFLPFSNKLEITRVNQSVFGDILFPEINFSEWDLINREDRGFYSFLTYLRKNQQF"
     gene            complement(4516..5697)
                     /gene="thyA"
                     /locus_tag="PLU55_02455"
     CDS             complement(4516..5697)
                     /gene="thyA"
                     /locus_tag="PLU55_02455"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPJ86949.1"
                     /translation="MVQQYLDYSLDILTSPYSNFKGGSKDSGIISLFGYQNRYDLTKG
                     FPLITTKQMPAKSKSMFTELLWFLKGNTNIEYMEKNNSPVWRADAFQHNLEKMTDAGI
                     YPKGMQKYSPEWTVAKENYGKRIKENHDFAMAWGDAGPIYPKQWRRWSKFSPLENQEQ
                     LYVKNKDGEFIPFEGKVYKEEQIDQVKNMIEGLKKKPTGKKHILTGWNVADLNDMSLP
                     PCHLLFQSTANENGEMEMELYQRSCDSFLGVPFNIAQYAALTQIIAKESGMTPKTFVH
                     TFGDAHFYTSIGERTMWYKNNFKEVQGKIRDARDLEKRTGDKSSYLEIVEWINKRAPA
                     DGNMEDYDHVTAILEQLANEPLGLCKLEIVDKPFDKLELNDFTIKDYKHHQRISRKMC
                     V"
     gene            complement(5760..6197)
                     /locus_tag="PLU55_02460"
     CDS             complement(5760..6197)
                     /locus_tag="PLU55_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86950.1"
                     /translation="MKEFQVTPQEKYDFCVCSVLQAILRNYNINLSQEEISKNLMPGK
                     NGGHMIDDASIGNFLFSNGFKYEHYFWNQTPFNEPDSLLEEMDKNEGFVGINKHVYIL
                     RNFKDPDLEMIDPVNGEFIRRSIFDVLKEMHDKDGGFGLIKKL"
     gene            complement(6199..6780)
                     /locus_tag="PLU55_02465"
     CDS             complement(6199..6780)
                     /locus_tag="PLU55_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86951.1"
                     /translation="MILSALKVLELNEKYNLIEGLSERELKNPEGSLLDLRIGQIHNI
                     IGESLLGVKERYSSKTETIGDVRKDGNKRFTIKPGEYYLVTTMEVVSSPAEKIKYDEN
                     FPEAYLIPKICPRSSLQRGGISLHFTSTNPGYKGQLTFGIKNLGNQDFIFELGARMFS
                     IEYYAVVGDIKRAYSGQHQGGRVTSNGKIEIQN"
     gene            complement(6782..7396)
                     /locus_tag="PLU55_02470"
     CDS             complement(6782..7396)
                     /locus_tag="PLU55_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86952.1"
                     /translation="MRIIQEQPIKTRPTWDETFMSLAIQNSSRSSCWFIRSGSTFVQD
                     NQVISSGYNGASSRYKDNCLEIGCAKEKRGLKYEESLNSGYCIGIHSEMNGIGHLNKM
                     NPNKMTLYTTIFPCVTCAKNLEPYSIQRIVFKKKYLDDEFNYALEHLEKLGIQVDQLD
                     LSLRRFWDIGVYRKWKKKDIWTDEEIENARKLIDCWEDIFKKTN"
     gene            complement(7505..7831)
                     /locus_tag="PLU55_02475"
     CDS             complement(7505..7831)
                     /locus_tag="PLU55_02475"
                     /inference="COORDINATES: protein motif:HMM:NF015997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HPJ86953.1"
                     /translation="MHELATDIINKNIEKIIDNHSYENQKNVNPYGCICYGLDAKCHN
                     IENLNCFFCYCPNYDRTIPEGKCKIDSPDGKYIETINGRLWDCSDCTFPHKRENAIKL
                     LEKLFK"
     gene            complement(7855..10593)
                     /locus_tag="PLU55_02480"
     CDS             complement(7855..10593)
                     /locus_tag="PLU55_02480"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HPJ86954.1"
                     /translation="MGENLEEKTNYNPNIRKEYSYKEALEASKDYFNGDELAADTFLS
                     KYALKDSSRQKGNNSLASIVLYEKSPTEMHHRLASEFERIESKYPNPMKHEEFFELMD
                     HFKYIVPQGSPMAGIGNNHQIVSVSNCFVIGNDEKSSDSYGAIMKIDQEQVQLMKRRG
                     GVGHDLSYIRPKGMTVLNSALTSTGVVPFMERYSNSTREVAQDGRRGALMLTLSVQHP
                     DAEKFIDAKLEKGKVTGANISIKVTDDFMKAVYMNKDYQQKFPINSDTPKYTKEINAN
                     KLWNKIIENAWKSAEPGVIFWDTMLRESIPDCYADLGFRTMSTNPCGEIPLCPYDSCR
                     LLALNLAEYVNNPFTKEAKFDFDLFKKHVGYAQKMNDDIVDMEIEKIDSILKKVESDP
                     EDEETKYVEKRLWEKIKDKTIRGRRTGTGVTGEGDMLAALGLTYGTKEATEFATEVHK
                     TLALEAYRCSVQLAKERGKFEIYDSEREKNNPFIQRIKDADPALYEDMVKHGRRNISM
                     LTIAPTGTVSMMTQTTSGIEPVFSPVYDRKKKINQNDVGTRVDKIDEQGDKWQTYKVF
                     HHKFEDWLKINGYDVEEVKKIANESIVSPEKKEELEDILKKSPYYKATADDVDWVQRI
                     KMQGAIQKWVDHSISVTINVPENIDKTLVDKLYKTAWEEGCKGATIYRDGSRDGILNK
                     GKLEHINLVQNNVKAHPLLDIKPQAMKYRIKRTEADDSLHVILTSDLYVDDQNKKAYF
                     IPDEDFQVRAPGGAATSVTFAQSGMDRTEILRGPDPKYAEFVKRLQSPFSNEDEGIGP
                     RRIKSIEHAAGLIFEDYFLRNGILKRDEITGELIQNVRKKDLRKVERGTPEYNAIISQ
                     VNLSENDEEVEISGTNGKLDKKFLCGRCGSTEYHFEAGCHSPKCRNCGFDNGEGCG"
     gene            complement(10617..11054)
                     /gene="nrdR"
                     /locus_tag="PLU55_02485"
     CDS             complement(10617..11054)
                     /gene="nrdR"
                     /locus_tag="PLU55_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPJ86955.1"
                     /translation="MICPYCNYKESKVTDKRTSPDGIRRRRECLKCKKRFTTYERIER
                     TDLYVIKKDGRREKFDIKKLEAGIEKALEKRPIPQDKINKMISAIDEQIRKAGKKEVK
                     SSVIGELVMKKLKKLDDVAYVRFASVYRQFQDINDFKQEVNEL"
     gene            11282..11566
                     /locus_tag="PLU55_02490"
     CDS             11282..11566
                     /locus_tag="PLU55_02490"
                     /inference="COORDINATES: protein motif:HMM:NF039180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5652 family protein"
                     /protein_id="HPJ86956.1"
                     /translation="MDYLNELAAQLGVSLWLLVVIFIWSAVWKLMALWKSARKEHLVW
                     FIVLALFNSVGILEILYIYIFSEKPCCKLSKKQSTTKNKKSEKKSRKKRK"
     gene            complement(11589..11828)
                     /locus_tag="PLU55_02495"
     CDS             complement(11589..11828)
                     /locus_tag="PLU55_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86957.1"
                     /translation="MSKFKWVIWGSLFSLVLFFSIYFLIHVKVVNSVGPVVLMLGKMD
                     VSKLGIFFSDIKNVIITSLIVGFLLGLLTGTKSKN"
     gene            complement(11885..12106)
                     /locus_tag="PLU55_02500"
     CDS             complement(11885..12106)
                     /locus_tag="PLU55_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86958.1"
                     /translation="MGFKGWLIRGIICSLLVAVAVYLLELMQLFEIKIIIISILVIGF
                     LLSTLIGWAFKKKPTNSSKRVVVKGFAGR"
BASE COUNT         3832 a         2334 c         1370 g         4658 t
ORIGIN      
        1 gcttgctgtt ccatttctcc taatcttaat gctcctcctc ttgctctacc ttcaataggt
       61 tgtcttgtta aaagtgcaac tttaccagaa gctcttccat gcattttatt tcctaccata
      121 tactttaatt ttaagtaata taaatctcct acaaatatcc ttgaaactat tttctttcct
      181 gttattccat tatacattgt ttctttacca tcaaatctaa atcctaaatc ttcaagttgt
      241 tcttcgagtt ctttcttgct tgctccagag aaagaagtgc catcaacaat ctctcctctt
      301 aatgaaccca ctttacctgc aagtaattca agtaaatatc ctactgtcat tctcgaaggc
      361 aaaccgtgtg gattaaatat aatatcaggt ctcatgcctc ttgaagtaaa aggaacatct
      421 tcttcaggta caatcatacc aataactcct ttctgtccat gagatgttgc aaatttatcc
      481 ccgagctcag gaattctctg gtctcttgtt ttaacctgaa caattttatt tccttcatta
      541 tcttctgtaa taaacactga atcaataatt cctttttctt cttgcctcat tgccacactt
      601 gattctttct ttgttcttat tgaaatttct cttgcttcag ataagaattt tggaggagac
      661 atcttaccaa tcatgacctc tccttctgaa acttctgctt ctggagaaat aactccatca
      721 tcttctaaat gtctataact tgcttccatc ctataaccag aagcatcttt ttctggcata
      781 gcaatttcat cttttaatcc accagcataa ttcatttcaa ttgcagaata aggtctgaaa
      841 taaaaacttc ttccaagtcc tctatcaaga cttcctttat ttaatactaa agcatcttcc
      901 atattatatc cctcgtacgc cattatagct acaacaagat tttgtcctgc tggatatgta
      961 ttcaaagtat cataaacaaa acttcttact atcggtttct gtggatattg taaaatactt
     1021 acatcagtat ctaatctaca tagataattt gcagaatata atccaagagc ttgtttctgg
     1081 gtttttgaac ctctatttaa tcttgaactc tggtcgtgat taccatatgg aacaagactt
     1141 gtaacagttc caaacaatga aacaggatca atttcaagat gagtgtgttc ttgagttaaa
     1201 ctttcttcat taagtgcaac caaagcattt tcctcttcag cagcatccaa atattcaata
     1261 atcccttttt caataaggtc tttccatttg atttctcctt gttctatttg tactaggtgt
     1321 tcgctcttaa gtttagatgt cccattttca acaattatta aaggtcttaa aactcttcct
     1381 gattctgttg aaatataaat tgtttttatt ccttcatctt ctctgaaact catttgaaca
     1441 ggaagttgat tctctcttct tttttctctt atttgtttta caaatttatt tccatcattc
     1501 actgttccaa taaacatacc attaaaaaac acatcataat ttccaatatc ctctttattt
     1561 aattcaagag attccaaatc tttcatgaat tttgattcat ctaattcaac tcttgttgaa
     1621 actcttgcaa gtattgctaa attctttcta agaccaattt ctgttccttc tggtgtttca
     1681 atagggcaga atcttccata gtgagtagga tgaagagttc ttgccataaa attttcttga
     1741 tctccaggaa gcatgcttga aactctttgt aattgtgaaa ttgttgcaag gtaatttgtt
     1801 ttatcaatat tctgggttat accgcttctt tcaccagtcc atgaacctgt tgcaattgca
     1861 gattcaactc tatgagaaaa caaagttgat ttagctaata cttttataga aaagaatttc
     1921 tttctcttag aagatttttg aagagaatat tgtatatctc taactaaaat tcctaaattt
     1981 actctaaata atgtagcaag taaatctcca gataatctaa ctctcttatt tgcataatgg
     2041 tctttatcag ttcttaatct tggattttct ttagcgacta aaaattgttt aataaattta
     2101 caaagagtta cagctttttt cattctgtct tcttttttct gtccaatatg agggaataaa
     2161 tacgaatcaa ttctttgttt aactctgtca agaatttctt tctttgtacc ctgaagattt
     2221 gtcttttcag caataaacat catagcatct tctttttcag ccatgttagt atattcatat
     2281 aaattaacaa taacactatc tgtttcttta ccaatatatt ttgcaatatc agattctttt
     2341 gttaagccta atgctttaag taaaacaatt gcaggtatat ccttgaatct gctgaaagaa
     2401 acattaaaga taccttcctt gttttcagtt attgttatag gtatcctata agttcctttt
     2461 aatgagaata atttaagggt caaatctcct tttttgttat tttctatgaa aggttgattt
     2521 tcagctaaat cttctgccat aatcatcact ctttcatttc ctttaattat aaaataccct
     2581 cctggatcta aagggtcgtt gtaatttgca ataaggtctt cttttgacat tcccatagta
     2641 ttacaatttt tactcttaac cataattggt attcttccaa tttccacaac ttctgaatca
     2701 atctgtccgt ctttttttac agtaagttca agggttattg gagcagcata tgtaaggttt
     2761 cttaattgag cttcagcagg agttatcaaa gaagcacttc catcagcttc tgttactctt
     2821 ggttttccaa cttcaatttt accgagggtt atctcaaatt cttcatttgt aattgtttca
     2881 gaaatttcat ttacaatctc ttgaagtttt ttttctatga aattattaaa agaaataata
     2941 ttagattcaa ctaatgaatg ttcattcaag taatttttaa cgattatgtg tccttgtttt
     3001 gccatttaat ttttaaacta caactctgaa atacaaattt attttatctt cttcttttct
     3061 ttcaatcttt ataatatctc caacagcaca attcatatct gccaaggcag catcattgag
     3121 aagaattttt ggtaattgag atttagatat attatatttc tttaacaatt cttgaacttc
     3181 tttttcgtta agttttgtgt gttttggttg tagaatgtgc atcttgaatg tgtaatgtca
     3241 tttaatcctc agcacactgg ccacaggata aatatttaac aactttgtga taataaaaga
     3301 taacaaaaat tggtttttaa acctttgcat taaggttata cactacatcc attcatagta
     3361 ctactaaaat ttacaaaaaa tggccccgat gggatttggg cctcacgtac gctcgaccta
     3421 acacctcata aattcgatgt taaacccaaa ggttcaaatc aaatttaaaa aattaaatta
     3481 aagttaatat ggccccgatg ggatttgaac ccacgacacc tggattaaaa gtccagtgct
     3541 ctaaccaggc tgagctacgg agccatgatg taacagcttc ataatctcag aatggtttgt
     3601 tcgttagaac tttaaccagg ctgagctacg gagccatacc tcagaaaaag ataaaatcac
     3661 tttataaaat taaccaatac agaatatatc atcctgcatt aaagaaattt ttgtattttt
     3721 tcactaaatt cttcttcact catagctcca acaaagttag tgataacttt tccatcttta
     3781 aacaaaacaa aattaggaat agacgaaaca ccaaatttct gtgaaatctc atgtccatct
     3841 tcaacattaa ccttacctac ttttaatttt ccattaaatt tctctgcaac ctcttcaaca
     3901 ataggcccca tcataacaca aggcatacac caatcagcat aaaagtcaat taagacagtt
     3961 ccttctttga taaattcatt aaaactacca tttgttaatt ctgccaccat gttccatata
     4021 caaatgttct atttttaaaa ttgttggttt tttcttaaat aagttaagaa agaataaaat
     4081 cctctatctt ctcgatttat taaatcccat tcagaaaaat taatttctgg aaaaagaata
     4141 tctccaaaaa cactttgatt aactctagtt atttctaatt tatttgaaaa aggaaggaat
     4201 tttttgtaaa tctctcctcc accaattata aaagaatctt tattgtctgt aaaaccaatt
     4261 gcctgttcta tgtttcttgc tagatatatt ccactttgtt catttaatgt atttgaaaga
     4321 acaatatttt ttcttccagg taaaggctta aatttttcac taagagattc gtaagttttt
     4381 cttcccataa taacaggatg attaaatgta atatctttga atctttttat atcttcagga
     4441 atataccacg gaatttctcc gtattttcct ataacattat ttttagaaat tgctgcgatt
     4501 atagtcaaat tcattttaaa cacacatttt tcttgatatt ctctgatggt gtttataatc
     4561 tttaatagta aaatcattaa gttctaattt atcaaaaggt ttatctacaa tctcaagttt
     4621 acaaagccct aaaggttcat ttgctaattg ttcaagaatt gctgttacat ggtcgtaatc
     4681 ttccatattt ccgtctgcag gggcacgttt attaatccat tcaacaattt ctaaataact
     4741 tgatttgtca cctgttcttt tttctaaatc tcttgcatct cttatttttc cttgaacctc
     4801 tttaaaatta tttttatacc acattgttct ttctcctatt gaagtataaa aatgagcatc
     4861 tccaaatgta tgaacaaatg ttttaggagt catacctgat tctttagcta taatttgtgt
     4921 caatgctgca tactgtgcaa tattaaatgg aacacccaaa aatgaatcac aactcctttg
     4981 atagagttcc atctccattt ccccattctc atttgcagtt gattgaaaca aaaggtgaca
     5041 tggaggcaga gacatatcat ttaaatctgc aacattccat ccagttaaaa tatgtttctt
     5101 gcctgttggt tttttcttta aaccctcaat catatttttt acttggtcaa tttgttcttc
     5161 tttgtaaact ttaccttcaa atggaataaa ctctccatct ttatttttca catataattg
     5221 ttcttgattt tctaaaggag aaaatttact ccaccttctc cactgctttg gataaatagg
     5281 tccagcatct ccccaagcca tagcaaaatc atgattttct ttaatccttt ttccataatt
     5341 ttcttttgca acagtccatt ctggagaata tttttgcatt cccttaggat aaattcctgc
     5401 atcagtcatt ttctcaagat tatgttgaaa agcatctgct ctccaaacag gagaattatt
     5461 cttttccatg tattcaatat ttgtatttcc ttttaaaaac cacaataact ctgtaaacat
     5521 ggatttagat ttagcaggca tttgttttgt tgtgattaaa ggaaatcctt tagtcaaatc
     5581 atatctattt tgataaccaa ataaagaaat aattccagaa tctttgcttc ctcctttaaa
     5641 attagaatat ggagaagtta aaatatctaa tgaataatct aaatactgtt gcaccatttt
     5701 tattataaaa ctaaaataat tttcttttat aaatacagtt gaattccaat gtatatattt
     5761 tacaacttct ttattaatcc aaaacctccg tctttatcat gcatttcctt aagaacatca
     5821 aatatactcc ttctaataaa ttctccatta actgggtcaa tcatttccaa atcagggtct
     5881 ttgaaatttc tcaaaatata tacatgttta tttattccta caaatccctc gtttttatcc
     5941 atctcttcaa gtaaactatc tggttcattg aagggagttt gattccaaaa ataatgttca
     6001 tatttgaaac cgtttgaaaa caaaaaattg cctatgcttg catcatcaat catatgtcct
     6061 ccatttttac caggcattaa gttttttgaa atctcttctt gagaaaggtt tatattataa
     6121 tttcttaaaa tagcttgcaa aacagaacaa acacaaaaat catatttttc ttgaggagtc
     6181 acttgaaatt ctttcatttt aattttgaat ttcaatctta ccgttgctgg taactctccc
     6241 cccttgatgt tgtccagaat aggctctttt aatatctccg acaacagcat aatattctat
     6301 actaaacatt cttgcaccta attcaaaaat aaaatcttga tttcctaaat tttttattcc
     6361 aaaagttaat tgtcctttat atccgggatt agttgaagtg aaatgcaaac ttattcctcc
     6421 tctctgtaat gaacttctag gacaaatctt aggtattaaa tatgcttcag gaaaattttc
     6481 atcatatttt attttctcag caggagaaga aactacttcc attgttgtta ccaaataata
     6541 ttctccaggt ttaattgtaa atcttttatt cccatctttt ctaacatctc caatagtttc
     6601 ggtcttagaa gaatatcttt ctttaactcc aagcaaactc tcgccaataa tattatgaat
     6661 ctgtccaatc cttaaatcca aaagactccc ttcaggattt ttcaattctc tctcacttaa
     6721 accctcaatt aagttgtatt tttcatttaa ctcaagaact tttaatgcag ataatatcat
     6781 tctaattagt ttttttaaaa atatcctccc aacaatcaat tagttttctt gcattttcta
     6841 tttcctcatc tgtccatata tccttctttt tccatttacg atatacacca atatcccaaa
     6901 atcttctcaa gctcaaatca agttgatcta cttgtattcc taatttttct aaatgctcta
     6961 aagcataatt aaattcatca tcaagatatt tcttcttaaa tacaattctt tgaatagaat
     7021 atggttccaa atttttagca caagtaacac aaggaaatat tgtggtataa agtgtcattt
     7081 tatttggatt cattttattt aaatgtccaa ttccattcat ctcagaatga attcctatac
     7141 aataaccaga attaagagat tcttcatatt ttaatcctct tttttcttta gcacaaccaa
     7201 tttcaagaca attatcttta tatcttgatg atgctccatt gtatcctgaa gatataactt
     7261 gattatcttg aacaaacgta cttccagacc tgataaacca acaagaagaa cgagaagaat
     7321 tttgtattgc taaactcata aaagtctcat cccaagtagg tcttgtctta attggttgtt
     7381 cctgaataat cctcatttta atttattgta aaacttagca gttttatttt ataagcataa
     7441 ttatacttca aaatataatt caataaaatt tatccacaaa atatgcaatt atcaaaacca
     7501 caattcattt aaacaatttt tctagtaatt taattgcatt ttctctctta tgaggaaaag
     7561 tgcagtcaga acaatcccat aatctaccat taatagtttc aatatatttt ccatcaggag
     7621 aatctatctt acactttcct tctggaattg ttctatcata atttggacaa taacaaaaaa
     7681 agcaatttaa attttcaata ttatgacact tcgcatccag cccataacaa atacatccat
     7741 aaggatttac atttttttga ttttcatagc tatgattgtc aataatcttc tcaatatttt
     7801 tattaataat atcagttgct aattcgtgca ttttaaacaa tttatgctcc acatttatcc
     7861 acaaccctcg ccattatcaa aaccacaatt cctacattta ggagaatgac atcctgcttc
     7921 aaaatgatac tcagttgaac cacatctccc acataaaaat ttcttatcca atttaccatt
     7981 agttccagat atttcaactt cttcatcatt ttcagacaaa ttaacttgag agattattgc
     8041 attatattca ggtgttcctc tctcaacttt tcttaaatcc ttttttctaa cattctgaat
     8101 caattctcct gttatctcat ctctcttaag aatcccgttt ctcaagaaat aatcttcaaa
     8161 tattaaacct gcagcatgtt caatcgactt tattcttcta ggaccaattc cttcatcttc
     8221 atttgagaat ggagattgta acctcttaac aaattcagca tattttggat caggacctct
     8281 caatatttct gttctatcca taccagattg agcaaatgta acacttgtag ctgctccccc
     8341 tggagctcta acttggaaat cttcatctgg tataaaatat gcttttttgt tttggtcatc
     8401 aacatataaa tcacttgtta gtatgacatg caaactatca tctgcttcag tcctcttaat
     8461 tctatacttc attgcctgag gtttaatatc tagcaaagga tgagctttaa cattattttg
     8521 aacaagatta atatgttcca attttccttt attaagaatt ccatctcttg aaccatctct
     8581 gtaaatagta gctcccttac atccctcttc ccaagcagtc ttgtatagtt tatcaactaa
     8641 agttttatca atattctcag ggacattaat tgtaacactt atagaatgat ccacccactt
     8701 ttgtattgct ccctgcatct ttatcctttg aacccagtca acatcatctg cagttgcctt
     8761 ataataagga gattttttta atatatcctc taattcttct tttttttctg ggctaacaat
     8821 actttcatta gctatttttt taacctcttc aacatcataa ccattaatct taagccaatc
     8881 ttcaaattta tgatgaaata ctttgtatgt ttgccattta tctccttgtt catcaatttt
     8941 atctacacgt gtaccaacat cattttgatt tattttcttt tttctatcat acactggtga
     9001 aaatacgggt tcaatccccg aagttgtctg agtcatcata ctcactgtac ctgttggagc
     9061 aattgtaagc atagaaatat ttctccttcc atgttttacc atatcttcat aaagtgcagg
     9121 atcagcatct tttatccttt gtataaatgg attatttttc tctctttcag aatcataaat
     9181 ctcaaacttt cctctttctt ttgctaattg aactgaacat ctatatgctt caagagcaag
     9241 agtcttatga acttctgttg caaattctgt tgcttctttt gtaccatatg ttaaacctaa
     9301 agcagcaagc atatctcctt ctcctgttac tcctgtacct gttcttcttc cacgaattgt
     9361 cttgtctttt attttctccc acaatctttt ctcaacatat tttgtttctt catcctcagg
     9421 atcactctca acttttttta aaatactatc aattttctca atctccatat ccactatatc
     9481 atcattcatc ttttgagcat acccaacatg tttcttaaat aaatcaaaat caaattttgc
     9541 ttcttttgta aaaggattat taacatactc tgccaaatta agagccaata acctacacga
     9601 atcatatgga cataatggaa tctcaccaca aggatttgtt gacatagttc taaatcctaa
     9661 atcagcataa caatcaggta ttgattctct aagcatagta tcccaaaaaa ttactccagg
     9721 ttcagcagat ttccaagcat tttcaattat cttattccat aatttatttg cattaatttc
     9781 tttagtatat ttaggagtat ctgaatttat tggaaacttc tgttgataat ctttattcat
     9841 ataaactgct ttcataaaat catcagttac tttaatggaa atatttgctc cagtaacctt
     9901 tcctttttca agtttagcgt ctataaattt ttcagcatca ggatgttgaa cagacaaagt
     9961 taacatcaat gcacctctcc ttccatcctg agcaacttct cttgttgaat tagaatatct
    10021 ttccataaac ggaacaactc cagtagaagt taaagcacta ttaagaacag tcatcccttt
    10081 tggtcttata taagataaat catgtccaac tcctcctctt cttttcatta attgaacttg
    10141 ttcctgatca attttcatta ttgcaccata tgagtctgaa gatttctcat catttcctat
    10201 gacaaaacaa ttagatactg aaacaatctg atggttattt cctatacctg ccattgggct
    10261 tccctgagga acaatgtatt tgaaatggtc cattaactca aaaaattcct catgtttcat
    10321 tggattagga tatttacttt caatcctttc aaattcactt gctaatctat gatgcatctc
    10381 tgttggactt ttctcataaa gaactatact tgctaaacta ttattacctt tttgtctaga
    10441 agaatcttta agtgcatatt tactcaaaaa agtatctgct gcaagttcat caccattaaa
    10501 ataatctttt gaagcttcta atgcttcttt ataactatat tcctttctta tatttggatt
    10561 gtagtttgtt ttttcttcta aattttcacc cattttgcct ctcttttgtc tataatttat
    10621 aattcattaa cctcctgttt aaaatcattt atgtcctgga attgtctata tactgatgca
    10681 aatcttacat aagctacatc atctagtttt ttcagtttct tcattactaa ttcacctata
    10741 actgaactct taacttcttt ttttcctgct ttccttatct gttcatctat tgcacttatc
    10801 atcttgttta ttttatcctg cgggataggt cttttttcaa gggccttttc aattcctgct
    10861 tcaagttttt taatatcaaa tttttctctg cgaccatctt tttttataac atataaatca
    10921 gtcctttcaa ttctttcata tgttgtaaat ctttttttgc attttaagca ctctctcctc
    10981 cttcttatcc catcaggaga agttctttta tctgtaacct ttgattcttt ataattacaa
    11041 tacggacata tcatatctta ttttaccccc ttaaaccata tacaatatat tgtatatgac
    11101 atccctctta attaccacct attgtagtta agatgaagtt cataaaaagc acttatattt
    11161 aaactttagt aatcactaat tccttcaaca ccaacataca actttttcca tagaaaattt
    11221 taataaaaag ctttaccaac atttttaaag tttagaatta tcttaaaaat aggaggtgaa
    11281 aatggattat ttaaatgaac ttgctgctca attaggagtt tctctttggc tattagtggt
    11341 catatttata tggtctgcag tctggaaact tatggcgctt tggaaatctg ctagaaaaga
    11401 gcatcttgtt tggttcattg tacttgcttt atttaacagc gttggtatat tagagatatt
    11461 gtatatctac atcttctctg agaaaccatg ttgtaaatta tctaaaaagc aaagtacaac
    11521 taaaaataag aaatcagaaa agaaatcacg aaaaaaaaga aaataatttt tttaattttt
    11581 aatttttatt agttctttga ttttgttcca gttagtaatc ctaataagaa accaactatc
    11641 aaagaagtta ttataacatt ctttatatct gaaaaaaata tccctaactt ggaaacatcc
    11701 atttttccaa gcattaaaac aacaggaccc acactattca caacctttac atgaataaga
    11761 aagtaaatag aaaaaaacaa aacaagagaa aaaagacttc cccatataac ccatttaaac
    11821 ttagacatat ttctttaatt acctctttct tttaatattt ttctctatat aatgtaaata
    11881 taaatcatct tcctgcaaat ccttttacaa caaccctctt agaagaatta gttggtttct
    11941 ttttgaaagc ccatccaatt aaagtagaca ataaaaatcc aataaccaaa attgagatta
    12001 ttattatttt aatctcaaat agttgcatta attctaaaag ataaacagca actgcaacaa
    12061 gcaaagaaca aattattcct cttatcaacc accccttaaa tcccatttta atatcaagta
    12121 aaggatattt ttaaacttaa ctaaaaaatt aattaaataa aatttaaata agttatctta
    12181 cttaaataaa tatg
//
LOCUS       DAOPCH010000022        11946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_5304_length_11946_cov_4.799765, whole genome shotgun sequence.
ACCESSION   DAOPCH010000022 DAOPCH010000000
VERSION     DAOPCH010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 11946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11946
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5304_length_11946_cov_4.799765"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLU55_02505"
     CDS             complement(<1..278)
                     /locus_tag="PLU55_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86959.1"
                     /translation="MVENKKNSLGKLKVLCVCGLGLNRSRYLAKYLGEKGYDTKWRGL
                     ESCDFNLESKNLIGIKDIEWADIIVVSKEKYKSILNDNFKVRDKKIVC"
     gene            complement(265..1140)
                     /locus_tag="PLU55_02510"
     CDS             complement(265..1140)
                     /locus_tag="PLU55_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPJ86960.1"
                     /translation="MKTQESILKEKLGLSDKDVIKLKNFGRQTRNANYVLELNGDPSY
                     FLKVNSEGIKKEIRMFDFLEKHKVFSTLMPVYKNEHIIILPFVKDLRDTEVRDNLDFI
                     LGYNNKALALDGSVFDNYKSDKLFENHYVKKFINRLDRHADLVRNFWKDIEGLKKFYG
                     ENPQECFESLPKILVHGDIQHKNLQSDSQGNIYLIDFEDVYFDSPSWDLSRPLMDLEP
                     REIEDYKENYIEGVAIGNKDLLRKAINRDFVVRIITDSIGRQQRFGIEEAKTYLDIYR
                     ERYTNNLEALIYGGK"
     gene            complement(1091..2209)
                     /locus_tag="PLU55_02515"
     CDS             complement(1091..2209)
                     /locus_tag="PLU55_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPJ86961.1"
                     /translation="MKENSGNQEIRKLDFSLISKVTENCNLNCGYCYLAEKKNLKMSE
                     EVAESLIKSFLKFNNDFAHFTWIGGEPLLMPDSFFTKIMELEEKYNYKNIPISNSIQT
                     NGIALTPARFDRLKDLGYKIGISYDGAPDLQNQRMTESQTNKLLEMIEYAKGKAGLLA
                     VLTKNTIGREKEIYDFFREYTTWAKVNFYSPTGHGAEKEDELMLNPDEASKMMVNLYD
                     LWKNDSSPLELRPMLENVRSFFTGFPVACDFSAVSCYKILGSDTKGDIYPCSKAMNFP
                     YLKMGNINEGLDNLVGNKIHKMPLERYIGLKKKGGNPWLHLSSGGCPIEALSHKGDYM
                     KATTYSEGVRNTLFERIKEDMQNENTRKHLERKVGLIR"
     gene            2430..2987
                     /locus_tag="PLU55_02520"
     CDS             2430..2987
                     /locus_tag="PLU55_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPJ86962.1"
                     /translation="MLLNNKELRILKEFAGDYSFKNYGRKLASKLNMNQKTVSNILNT
                     LEKENILKFTQEGKNKYYYLNRLNPYIKDIIKFLEINKKIDFLEKHKKINTLFLEIEK
                     RSKGIIGVFGSYASFSETKTSDLDLFVIGDIQEVKDLERMYNIKLNIVKLDKNKFKYK
                     DNFINEIIQNHVIIKGIEEYIELIW"
     gene            3074..4228
                     /locus_tag="PLU55_02525"
     CDS             3074..4228
                     /locus_tag="PLU55_02525"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPJ86963.1"
                     /translation="MRTKTKDKKIFDYSIDADSETLHQFKDCYSEDFVTAAALMPDAH
                     KGYSAPIGAVLITKGKIVPAWVGYDIGCGLIAVRIKGKDILKKIQDNKQKIYSEVMRT
                     IPMGVGEYQNPKNLSEKTKQEFKKLIEKFQKGPYDKDILHFLKNTSLKHLGTLGGGNH
                     FIEIGFYKKEVWLIIHSGSRGIGHRVAKRYMIKASNSKDNFERTFPLDVNSQLGKEYL
                     NILDFGLEYALLNRLEISYKVIESLEKVLNEKLTTELWVNKNHNHAIYEKGYYIHRKG
                     ATPAKKQEKGIIPGNMRDGSFLVQGLGNPKFLNSSSHGAGRALSRKDAKRKISLDDFK
                     KSMQGIQGTISEKTLDESPMAYKDINKIMEAQKKSIKIIKYIKPLINWKG"
     gene            4269..4802
                     /locus_tag="PLU55_02530"
     CDS             4269..4802
                     /locus_tag="PLU55_02530"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPJ86964.1"
                     /translation="MKMKLKILKPFFENPNEEFQIRELSRLLNINHTTIRQYLLKLVE
                     EEVIEIKKGKPYDYFKATINKKFLNLKLFFNLEKLRKSKLIEEIEKDYDYPTIILFGS
                     YASATDDCKSDIDLFLLTEIKKEKDYSKFKNKLNREITLHIFSKSGFNLAKNKNPELL
                     NNILNGITLSGKLEVFQ"
     gene            complement(4829..5284)
                     /locus_tag="PLU55_02535"
     CDS             complement(4829..5284)
                     /locus_tag="PLU55_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86965.1"
                     /translation="MVNSRIKKNKRGWVRLLEAFIAIVLLTSVLVVVSKTNSSPRNEL
                     QERISIIQTSIIRDIQLNEELRAAILSVNVADPVEWEDFETSGLSGVKERIEELAPKD
                     MECSAQICEINNPCSLKDVSKKEVYAKSGIISADLNTYSPRQIKMFCIR"
     gene            complement(5278..5979)
                     /locus_tag="PLU55_02540"
     CDS             complement(5278..5979)
                     /locus_tag="PLU55_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86966.1"
                     /translation="MNKKGTHVEVIISFVIFITFVVFLFVASKPAIIKQEDKTNLLDN
                     LEYKILEKEISADVKVISVTISSVTGNCVNLGNFLEGQDIDGRVVVKNSSGDSVEAHM
                     EGNSFSIDSKDTFFKIYYSEEFDLIADSGATCTPATPNVGLVKNNRYVLEEKVYTSLN
                     KEYEQLKEELGVGGGEKIDFTYGIVLSNGTIIEKEHPELKSNISTSIYVRETPIEYVN
                     KDYEIVQGYLRTTIW"
     gene            complement(6027..7028)
                     /locus_tag="PLU55_02545"
     CDS             complement(6027..7028)
                     /locus_tag="PLU55_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional N(6)-L-threonylcarbamoyladenine
                     synthase/serine/threonine protein kinase"
                     /protein_id="HPJ86967.1"
                     /translation="MTVILGIESTAHTIGIGIVENEKILANVRDMYTTEQGGIIPSES
                     AKHHERVFQELYEKALETADIEEEDIDAIAFSQGPGLAPCLLVGLRKAKEISLKLNKP
                     VVGVNHCVAHLEIGKITGAKDPVMLYTSGANTQIIAYASGKYRVFGETLDIGVGNFID
                     NFARYSGIGFPGGPVVEKLAKKGKNYIELPYKVKGMDIALSGILTNLKQKVESGKYSK
                     EDLAYSLQETVFAMLVETAERALAHTGKKELLLGGGVACNSRLQEMCRIMCDEREDGV
                     KFFCPEKSLLVDNGAMIAFLGEIMFKAKSNVFKKENLKDLDINASERTDDIEVDWKN"
     gene            complement(7107..7262)
                     /locus_tag="PLU55_02550"
     CDS             complement(7107..7262)
                     /locus_tag="PLU55_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86968.1"
                     /translation="MGTVLVKNVVKRKPGYLYYVDGKGNVCEAKMARGGKKKKKAAKK
                     TTKKKKR"
     gene            complement(7343..7696)
                     /locus_tag="PLU55_02555"
     CDS             complement(7343..7696)
                     /locus_tag="PLU55_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPJ86969.1"
                     /translation="MQVIIKDKSKNIKIKDVKKLSEFGKAIGLMFRSRENCPAMLFEF
                     SKPTRMRIHSFFVFFKFGAVWLDDKNKIIEKKIVKPFLPSIVPKKRFYKLVEIPLNKK
                     YKKEIKILFKKSNYS"
     gene            complement(7751..8158)
                     /locus_tag="PLU55_02560"
     CDS             complement(7751..8158)
                     /locus_tag="PLU55_02560"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative metallopeptidase"
                     /protein_id="HPJ86970.1"
                     /translation="MRYEEAPDLQIIAEDISKTLFPHIVMERVKCYRSYGSSTKGTIA
                     RCHALGKLMQKAIGVKAYYALEFLSARFDKLSKEDQMKTIIHELMHIPQTFGGGFRHH
                     NHVTEKNVNLMYRTYLSKKEGRDKFEKKSGWFN"
     gene            complement(8161..8379)
                     /locus_tag="PLU55_02565"
     CDS             complement(8161..8379)
                     /locus_tag="PLU55_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86971.1"
                     /translation="MKISIKVRFNSTYPKFEKVMPGKYILYIPEPQTDESEKFIKGLL
                     SNKLGVRKENISLINIEKDGKWNFHIGE"
     gene            complement(8441..8653)
                     /locus_tag="PLU55_02570"
     CDS             complement(8441..8653)
                     /locus_tag="PLU55_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017651940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HPJ86972.1"
                     /translation="MKVKIKLHPNSSQEKIKEVEKDKSYEVWIKEKPIDGKANKELIK
                     ILKKHFKKDVKITSGFTSRDKIIELS"
     gene            complement(8657..9847)
                     /locus_tag="PLU55_02575"
     CDS             complement(8657..9847)
                     /locus_tag="PLU55_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013748622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPJ86973.1"
                     /translation="MLIGLVGKPSVGKSTFFKASTLAEVEIASYPFTTIKPNHGIGYV
                     KVECLDKEFNTQCNPNHGFCINHQRFVPIDLMDVAGLVKGASEGKGLGNQFLDDLRGA
                     DVFIHIVDVSGTTDSEGKQVFDASYNPLEDIELLEDELNKWFCNILMKVWKTFARTTE
                     MSKAKFSEAVAKQFSGLKVKEDNVKDVLLRMNLPEKPTSWSEEQTINFAAELRKESKP
                     MIIAANKVDMENGEKNYKKIKEKYKDYLVIPCSADSELALRQATKAGLIEYIPGEREF
                     KILKELNPKQKEALEKIKQNVLDKFEYGTGVQQVLNSAVFDFLKYIAIFPASANKLGD
                     SKGNILPDCFLMPPGTTALDFAYSLHTDFGKNFIKAIDARTKMAWGKEHKLKHRDGLE
                     IVTR"
     gene            complement(9854..10414)
                     /locus_tag="PLU55_02580"
     CDS             complement(9854..10414)
                     /locus_tag="PLU55_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPJ86974.1"
                     /translation="MIIGLTGRIAGGKGVASDFFRAKGFEYLSLSNEVREEANKRGLP
                     LERKSLQDLGNLMRKEEGSSVLAKRVIKKIKKKKNYVIDGIRNSGEVEELRKVFKSKF
                     ILISIDADLRIRWKNAKNRAKESDPKTFKEFLKADKRDFKENSQNGQQVKKCMEMADY
                     ALLNNGTIQELIAQLKLVYRQLNLDD"
     gene            10557..10628
                     /locus_tag="PLU55_02585"
     tRNA            10557..10628
                     /locus_tag="PLU55_02585"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10590..10592,aa:Arg,seq:gcg)
     gene            complement(10854..11168)
                     /locus_tag="PLU55_02590"
     CDS             complement(10854..11168)
                     /locus_tag="PLU55_02590"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HPJ86975.1"
                     /translation="MDLNYNLYLERANNEIKFSEIGLIISNNKNLQTDIFKIEQPETF
                     YNSVISHAYYCIFYSAKAYLAKKGIKTEPPEEHKKTYNEFKKLVFQGIVDKELLKMRM
                     LS"
     gene            complement(11152..11772)
                     /locus_tag="PLU55_02595"
     CDS             complement(11152..11772)
                     /locus_tag="PLU55_02595"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPJ86976.1"
                     /translation="MLTKKQLKILNTFQKNKFKKLTWKQVKELSRENSSSVIQDAIKA
                     FLNEELITEEKIGTSKIYAVNHKNNKVYTYFEIYNKENLSKQVLKSIKELEDSLDKHT
                     YFYSIVIFGSFVSGEQKKDSDLDIAVFIEQENKRKIVEAVFKSMETKSLLKIDGHVIT
                     KDEFLEMLNVDYENLGKEIARKHLIVHNPAIFYSMLNDGIKHGFKL"
BASE COUNT         4017 a         2064 c         1370 g         4495 t
ORIGIN      
        1 aaacatacta tctttttatc tctcacctta aaattatcat ttaaaatcga tttatacttt
       61 tcttttgaga caactattat atctgcccat tcaatatctt ttattcctat caggttttta
      121 gattccaaat taaaatcgca agactccaaa cctctccact tagtatcata tcctttctct
      181 ccaaggtact ttgctaaata cctgcttcta ttcaatccca aaccacaaac acaaagaacc
      241 ttcagttttc ctaaactatt ttttttattt tccaccataa atcaatgcct ccaaattgtt
      301 agtatatctt tcacgataaa tatctagata agtttttgct tcttctatac caaatctctg
      361 ttgcctccca atagaatcag ttataattct aacaacaaaa tccctattaa tggctttcct
      421 caataaatct ttatttccaa tagcgacccc ctctatataa ttctctttat aatcctctat
      481 ttctctaggc tctaaatcca tcaaaggcct tgaaagatcc catgaaggag aatcaaaata
      541 aacatcctca aaatctatca aataaatatt tccttgagag tcactctgta gatttttatg
      601 ttgaatatct ccatgaacta atatcttagg caatgattca aaacattctt gagggttttc
      661 tccataaaac ttttttagcc cttcaatatc tttccaaaaa tttctaacta aatcagcatg
      721 cctatccaac ctgttgataa attttttaac ataatgattt tcaaacaact tatcactttt
      781 ataattatca aatacactcc catccaaagc aagagcttta ttattataac ctaaaataaa
      841 atccaaattg tctctcactt ctgtatctct taaatcttta acaaatggta gaataattat
      901 atgctcgttc ttgtatactg gcatcaaagt agaaaacacc ttatgcttct ccaagaaatc
      961 aaacatccta atctcttttt ttataccctc tgaattaact tttaaaaagt aagaaggatc
     1021 accatttaac tctaagacat aattagcatt cctagtttgt ctcccaaaat tttttaattt
     1081 aattacgtct ttatctgata agcccaactt ttctttcaag atgctttctt gtgttttcat
     1141 tttgcatatc ttctttaatc ctttcaaata aagtatttct tactccttcc gaatatgttg
     1201 ttgctttcat ataatctccc ttatgagata atgcttctat tggacaaccc cccgaagaca
     1261 aatgcaacca aggatttcct ccttttttct ttaaaccaat atacctttcc aaaggcatct
     1321 tatggatctt attccctacc aaattgtcta atccttcatt gatatttccc attttcaaat
     1381 aaggaaaatt cattgccttt gaacaaggat aaatatctcc ttttgtatct gatcctaaaa
     1441 tcttataaca agagacagct gaaaaatcac aagccacagg aaagcccgta aaaaaagagc
     1501 gaacattttc taacataggt cttaattcta gaggggaact atcgtttttc caaagatcat
     1561 acaaattcac catcatctta gatgcttcat caggattcaa catcaattcg tcttcttttt
     1621 ctgcaccatg tcctgttgga gaataaaaat tgacttttgc ccaagtagta tattctctaa
     1681 aaaaatcata tatttccttt tccctcccaa tcgtattttt agtcaataca gccaaaaggc
     1741 cagcctttcc ttttgcatat tctatcatct ctaataactt atttgtttga gattcagtca
     1801 ttctttgatt ttgtaaatct ggagccccat cataacttat tccaatttta tatcctaagt
     1861 ccttaagcct atcaaatctt gcaggagtta gagcgatacc atttgtttga atagagttag
     1921 aaatgggaat atttttataa ttatattttt cttccaactc cataattttt gtaaaaaaag
     1981 aatctggcat caaaagaggc tctccaccta tccaagtaaa atgagcaaaa tcattattga
     2041 attttaaaaa agattttatt aaagactctg ctacttcttc agacattttt aggttctttt
     2101 tttctgcaag ataacaataa ccacaattta agttacaatt ttcagttact tttgagatta
     2161 aagaaaaatc caactttctt atctcctgat ttcctgagtt ttctttcatc attttctttc
     2221 aagaattagg tatcttgagg taccatcacc tcaaaatatt aagatggcac ttgtttaaat
     2281 atctttgttt tttaaaaact tacataataa aacacataat caataattat attccctttg
     2341 attttattgc gacttttttt gaaatttcta tattattaat caaaatgcat aatcaaaaga
     2401 tttaataata tattactcat ataagtaata tgttactcaa taataaagaa cttagaattt
     2461 taaaggagtt tgcaggagat tattctttca aaaattatgg gaggaagtta gcctctaaat
     2521 tgaacatgaa tcaaaaaaca gtctcaaaca ttctaaatac tcttgaaaaa gaaaacatac
     2581 tcaaatttac acaagaaggt aaaaacaagt attattatct taatagactt aatccttata
     2641 taaaagatat tattaagttt ctagagataa ataaaaagat tgatttttta gagaaacata
     2701 aaaagataaa tacactcttt ttggaaattg aaaagagaag caaaggtatc attggtgttt
     2761 ttggcagcta tgcgagtttt tcagagacaa aaacttctga tcttgatttg tttgttattg
     2821 gagatattca agaagttaag gatttagaaa gaatgtataa cattaagttg aatattgtaa
     2881 aattagataa aaataaattt aagtacaaag acaattttat aaatgaaata attcagaatc
     2941 atgtaataat taaaggaatt gaagagtata ttgaattaat atggtaagta ttaaatggtg
     3001 ttgtaaatct catttattac tatgattaca tataaccgca ccatttataa gttttgtaat
     3061 aattcagtat ataatgagaa caaagacaaa agacaaaaaa atctttgatt attctataga
     3121 tgcagattca gaaacacttc atcaattcaa agattgctat tctgaggatt ttgtaacagc
     3181 tgcagctcta atgccagatg cccacaaagg atattcagct ccaatagggg cagtccttat
     3241 aactaaagga aaaatagttc cagcttgggt aggctatgat attggttgtg gattaatagc
     3301 tgtaagaata aaaggaaaag acattttaaa gaaaattcag gacaacaaac aaaaaatcta
     3361 ttcagaggtt atgagaacaa ttccaatggg tgtaggagaa tatcaaaatc ctaaaaacct
     3421 ctctgaaaaa actaaacaag aattcaagaa attaattgaa aaattccaaa aaggcccata
     3481 tgacaaagac attcttcatt ttttgaaaaa tacttcactt aaacatttag gaactttagg
     3541 aggaggaaat cattttattg aaatagggtt ttacaaaaaa gaagtttggt taataataca
     3601 ttcaggaagc agaggaatag gacatagagt tgcaaaaaga tacatgataa aagcttcaaa
     3661 cagcaaagac aattttgaaa gaacttttcc attagatgta aactctcaac taggtaaaga
     3721 atatctgaat attcttgatt ttggattaga atatgcttta cttaacagat tagagataag
     3781 ttataaagta attgaatcat tagaaaaagt cttaaatgaa aaattaacaa cagaactctg
     3841 ggtaaataag aaccacaatc atgcaatcta tgaaaaagga tattatattc atagaaaagg
     3901 tgcaactcct gcaaaaaaac aagagaaagg aataattcca ggtaatatga gagacggttc
     3961 ttttttagtt cagggtttag gaaatcccaa attcttaaat tcttcttcac acggtgcagg
     4021 aagagccttg agtagaaaag acgctaaaag aaaaatatct ttagatgatt tcaaaaaatc
     4081 aatgcaagga attcaaggaa caatctctga aaaaactctt gatgaatctc caatggctta
     4141 caaagatata aacaaaatta tggaagctca gaaaaaatca attaaaataa taaaatacat
     4201 taaacctcta attaattgga aaggataatt ggaaatattt ataaacatct ggaagtatat
     4261 atttacacat gaaaatgaaa ttaaaaatat taaaaccttt ttttgagaat cctaatgaag
     4321 aatttcaaat aagagaactt tcaagattac ttaatataaa tcatactaca ataaggcaat
     4381 atttgcttaa actagttgaa gaagaggtta ttgaaataaa aaaaggcaaa ccctatgatt
     4441 atttcaaggc aactataaat aaaaaattct taaatttaaa attatttttt aatcttgaaa
     4501 agctcagaaa atccaaatta atagaagaaa ttgaaaaaga ttatgattac ccaactataa
     4561 ttttatttgg aagctatgct tctgcaacag atgattgcaa aagtgatatt gatttatttc
     4621 ttttaacaga gataaaaaaa gaaaaggatt attccaaatt taagaataaa ttaaatagag
     4681 aaataacact tcatattttt tcaaaatcag ggtttaattt agctaaaaac aaaaatcctg
     4741 aattattaaa taatatttta aatggaatta cattgtcagg aaaattggag gtctttcaat
     4801 gaactttgga acattaatca aagatggttc atctaataca aaacatcttt atttgcctag
     4861 gagaatatgt gtttaaatct gcagaaatta tacctgattt agcataaact tcttttttag
     4921 aaacatcttt aagagaacaa ggattattta tctcacatat ctgagcacta cactccatat
     4981 cttttggagc aagttcttct attctttctt taactcctga aagtccagaa gtttcaaaat
     5041 cttcccattc cacagggtct gcaacattca cagataatat cgctgctctt aattcttcat
     5101 ttaattgaat atctcttatt attgaagttt gtattattga gattctttcc tgcaattcat
     5161 ttcttggtga agaatttgtt tttgaaacaa caaccaaaac acttgttagc aaaactattg
     5221 ctatgaatgc ttctaataat cttacccatc ctcttttgtt ttttttaatt cttgaattta
     5281 ccatattgtt gttcttaaat aaccctgaac aatttcataa tctttattta catattcaat
     5341 tggagtctct cttacataaa tgcttgttga aatattgctt tttaattcag ggtgctcttt
     5401 ttcaattatt gttccatttg ataaaacaat tccatatgta aaatctatct tctctccccc
     5461 tccaacacct aactcttctt ttaattgctc atattcttta tttaaagaag tgtaaacctt
     5521 ttcttctaat acatatctgt tattttttac taaccctaca tttggtgtag caggtgtgca
     5581 cgttgctcca ctatctgcta tcaaatcaaa ttcctcagaa taataaattt taaagaaagt
     5641 atctttacta tctattgaaa agctgtttcc ttccatatgg gcttcaacac tatctccaga
     5701 agaatttttg acaacaactc ttccatctat atcctgtccc tcaagaaaat ttcccagatt
     5761 aacacaattt ccagtaactg aagaaattgt cacacttatc acttttacat ccgctgaaat
     5821 ttctttttcc agtattttat actctaaatt atctaataga tttgttttat cttcttgttt
     5881 tattatagca ggttttgaag caacaaacag aaaaactaca aatgttataa atatcacaaa
     5941 tgaaattata acttcaacat gtgttccttt tttattcatt ctttcctctt ttttataaaa
     6001 taattagata aatgaagttt ttaaaactaa tttttccaat caacttcaat atcatctgtt
     6061 ctctcagaag cattaatgtc caaatccttg agattttctt ttttgaaaac atttgatttt
     6121 gctttaaaca ttatctctcc taaaaatgca atcatagccc cattatccac caataaactt
     6181 ttttcaggac aaaagaattt tactccatct tctctttcat cacacattat cctgcacatt
     6241 tcctgcaaac gagaattgca tgcaactcct cctcctaata aaagttcttt ttttccagta
     6301 tgcgccaaag ccctctcagc agtttctacg agcatagcaa aaacagtttc ctgaagagaa
     6361 taagctaaat cttcttttga atattttcct gattcaactt tttgtttcag gttagttaat
     6421 attccagaga gtgcaatatc cattcctttc actttataag gcaattcaat ataatttttt
     6481 ccttttttag ctaatttctc aactacagga cctccaggaa atcctattcc agaatacctt
     6541 gcaaaattat caataaaatt tccaacacct atatctaatg tttcaccaaa aactctgtat
     6601 tttccagaag cataagctat tatctgagtg ttagcacctg aagtataaag catcacagga
     6661 tctttggctc cagttatttt tcctatctca agatgtgcaa cacaatgatt cactccaaca
     6721 actggcttgt ttaatttcaa tgaaatttct tttgcttttc ttaatccaac taaaagacag
     6781 ggtgctaaac ccggtccttg tgaaaatgca atagcatcta tatcttcttc ttctatatct
     6841 gctgtttcaa gtgctttttc atataattct tgaaaaactc tttcatggtg ctttgcactc
     6901 tcagatggaa ttattcctcc ttgttctgtt gtatacatat cacgcacatt tgcaagtatt
     6961 ttttcattct ctacaatacc tattccaata gtgtgtgcag ttgattcaat tcctaaaata
     7021 actgtcatta aaaaaaaaga tagaattagt atataaaatt aattaaaaaa taatttgaaa
     7081 gtaaaaataa aaagcattaa gcaactttat ctttttttct ttttagttgt ttttttagct
     7141 gctttctttt tcttctttcc gcctctagcc attttagctt cacatacatt tccctttccg
     7201 tctacatagt aaagatatcc tggctttctt tttacaacat ttttaaccaa aacggttccc
     7261 attttttaac ctcctttttt gtgagtatga tttatcatag ttgatattct ttttaaatct
     7321 ttcttcgacg ggaaataatc atctaagaat agttactctt tttaaataaa atttttattt
     7381 cttttttata ttttttattt aaaggtattt caacaagttt gtaaaaacgt ttttttggaa
     7441 ctatcgacgg taaaaaaggt tttacaatct tcttttcaat tatcttattt ttatcatcta
     7501 accatacagc tccaaattta aaaaatacaa aaaaagaatg aatcctcatt cttgtaggtt
     7561 ttgaaaattc aaaaagcatt gcaggacaat tttctcttga acgaaacatt aaccctatag
     7621 ctttgccaaa ctcagataat ttttttacat ctttaatttt tatattctta gacttatctt
     7681 ttattatcac ctgcatttta tattcaacaa atagtaattt ataattttat cctttatctc
     7741 ttacttaaca ttaattgaac catccactct tcttttcaaa tttatctctt ccttctttct
     7801 tagagagata tgttctatac ataagattta cattcttttc agttacatga ttatgatgcc
     7861 tgaatcctcc tccaaatgtc tgaggtatgt gcattaattc atgtataatt gttttcatct
     7921 ggtcttcttt gcttagttta tcaaatcttg cagataaaaa ttcaagagca taatatgcct
     7981 ttactcctat ggctttttgc attaatttac cgagagcatg acatcttgca attgtcccct
     8041 ttgtagaact cccataactt ctgtaacact taaccctttc cattactata tgaggaaaaa
     8101 gagttttaga aatatcttct gcaattattt gtaaatcagg agcctcttca tatctcattt
     8161 tcactctcca atgtgaaaat tccatttacc atctttttct atatttatca gagaaatatt
     8221 ctctttcctt acaccaagtt tatttgatag aagtcctttt atgaatttct cactctcatc
     8281 tgtctgcggt tctggaatat acaatatgta tttaccaggc ataacttttt caaatttagg
     8341 gtaagttgaa ttaaatctca cttttattga tattttcata ttcttttcac actactctcc
     8401 tttttaagaa tttttattaa caatctatta tatacctaca ttaactcaat tcaataatct
     8461 tatctcttga agtaaatcca ctagtaattt taacatcttt tttaaaatgc tttttcaaga
     8521 ttttaattag ttctttattt gctttaccat caataggttt ttcttttatc caaacttcat
     8581 aggacttatc tttctcaact tcctttattt tctcttgaga agaattagga tgaagtttga
     8641 tttttacttt cattttctac ctagtaacaa tctctaaacc atctctatgt tttaatttgt
     8701 gttctttacc ccaagccatt tttgttctag catctatggc ttttatgaaa ttctttccaa
     8761 aatcagtatg aagagaatat gcaaaatcaa gagcagttgt tccaggaggc attaagaaac
     8821 aatcaggaag tatatttccc ttactgtctc caagcttgtt tgcagatgca ggaaatattg
     8881 caatatattt aaggaaatca aatactgcag aattaagaac ttgttgaact cctgttccat
     8941 attcaaattt atctaaaaca ttttgtttta ttttttcaag tgcttctttc tgttttggat
     9001 taagctcctt taatatttta aattctcttt ctccaggaat atactcaatt aaaccagctt
     9061 tagtagcttg tcttaaagct aattcagaat ctgcactgca aggtataacc aaataatctt
     9121 tgtatttttc ttttatcttt ttgtaatttt tttctccatt ttccatatct actttatttg
     9181 cagcaataat cattggctta ctttcttttc ttaattctgc tgcaaagttt attgtctgtt
     9241 cttcactcca actagttggc ttttcaggaa gattcattct taataaaaca tccttaacat
     9301 tatcctcttt aacctttaat ccagaaaatt gtttagcaac agcttcagag aatttagctt
     9361 tagacatttc agttgtcctt gcaaaagttt tccagacttt cataagaata ttacaaaacc
     9421 atttatttaa ttcatcttca agtaattcta tatcttctaa aggattataa cttgcatcaa
     9481 atacttgttt tccttctgaa tcagttgtgc ctgaaacatc aactatatgt ataaatacat
     9541 ctgctcctct taaatcatct aaaaattgat ttcccaatcc cttcccttca ctcgcacctt
     9601 tcacaagtcc agcaacatcc attaaatcaa ttggaacaaa cctttgatga tttatacaga
     9661 atccatgatt tggattgcat tgagtattga attctttgtc aagacactca accttaacat
     9721 accctatacc atgattgggc tttattgtag tgaaaggata agaagcaatt tcaacttctg
     9781 ctagagtaga tgctttaaaa aaagtactct ttcctactga aggtttaccc actaaaccaa
     9841 ttaacatatt atcttaatcg tctaaattta actgtctata aactaatttt aattgagcta
     9901 ttaattcttg aattgttcca ttatttaaaa gagcataatc tgccatctcc atacattttt
     9961 tgacttgctg cccattctga ctgttttctt taaaatctct tttatctgct ttaagaaatt
    10021 ccttaaatgt tttagggtca ctctcctttg ccctgttttt agcatttttc caccttatcc
    10081 ttaaatcagc atcaattgaa attaagataa atttactttt gaaaactttt ctcagttctt
    10141 caacttctcc agaatttctt attccatcta taacatagtt ttttttcttt ttaattttct
    10201 ttataaccct tttagcaaga acagaagaac cttcttcttt tctcataaga tttcctaaat
    10261 cctgtaaaga ttttctttcc agaggaagtc ctcgtttatt tgcctcttct cttacttcat
    10321 tagataaaga caaatattca aatccctttg ccctaaagaa atcagaagca acaccttttc
    10381 ctcctgcaat tcttcctgta agtccaataa tcatagaata actttatgaa acaagttttt
    10441 atattttagt gtattagaat attggtacta aaataaatga aaagcttcta caaatacaga
    10501 taatctttaa gtatctatcc aaaaaattaa aaacatttaa ccctaataat tatcaagccc
    10561 ctgtagttca atggatagaa cctctcgttg cggtcgggaa aatcggagtt cgaatcttcg
    10621 cgggggcgtt ttatttgttg taggtaaatt gaaatttacc atttataaaa acatagaata
    10681 actttttata tattttagtt actcgcacag ctttcttata tgtttcaaga aagtcttggc
    10741 attctctaaa cttttatttg caggctccag attagcctga gataattttt gataagtaaa
    10801 ttctcctctc tttttctttt ctattttgaa tatccctaac aaagtatctg cttttatgat
    10861 aacatcctca tttttaacaa ttctttatca acaatccctt gaaaaactaa tttcttgaat
    10921 tcattatatg tttttttatg ttcttctggt ggttctgttt taattccttt cttggctaaa
    10981 taagctttag cagaataaaa aatacaataa taagcatgag aaataactga attgtagaat
    11041 gtttctggct gttcaatttt aaaaatatct gtttgtaaat tcttgttatt actaattatt
    11101 aatcctatct ctgaaaattt tatttcatta ttagctcttt caagatacaa attatagttt
    11161 aaatccatgt tttattccat catttaacat tgaatagaat atagctggat tatgaactat
    11221 taaatgtttt cttgctattt ctttgcctaa gttttcatag tctacattta gcatctctaa
    11281 aaattcgtct ttcgttataa catgcccatc tatctttaag agagatttgg tttccattga
    11341 tttaaaaaca gcttcaacta tttttctttt attttcttgt tcaataaaaa ctgctatatc
    11401 taaatcagaa tccttttttt gttctccaga aacaaaagaa ccaaaaataa caatagaata
    11461 aaaataagtg tgtttatcta aactatcttc taattcttta attgatttta aaacttgttt
    11521 tgacagattc tctttattat aaatttcaaa ataagtgtat actttattgt ttttgtgatt
    11581 aacagcataa atcttagaag tccctatttt ttcttcagtt attaattctt cattcaaaaa
    11641 tgccttgatt gcatcttgta taactgaact tgagttttcc ctcgataatt cttttacttg
    11701 cttccaagtt agttttttaa acttattttt ttggaaagta ttcaatattt ttaattgttt
    11761 ttttgttaac attttcttta atcaatttta gggtttattt atacctaatt ttaagtataa
    11821 ttatactctt ttttaagtat ataaatcctt tcaattatga agtttcatat agaagttcat
    11881 tcttatttaa attaatcaaa taaaattatt gatgaaaaag attaagcagc tttttttcta
    11941 aagtcg
//
LOCUS       DAOPCH010000023        11288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_5652_length_11288_cov_4.180005, whole genome shotgun sequence.
ACCESSION   DAOPCH010000023 DAOPCH010000000
VERSION     DAOPCH010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 11288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11288
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5652_length_11288_cov_4.180005"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PLU55_02600"
     CDS             <1..654
                     /locus_tag="PLU55_02600"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPJ86977.1"
                     /translation="TPFEKAKEQHELYKKSELEKTLKEEKRQKEEAEKRKFLEPYFLS
                     QDSLNSYIKQAYSNVKKWPNEFDKRLFRVMLRQESSYNVHAVSKSGYKGLGQIGPEVY
                     QTFRPEKFEALKDSITGQINEYALQKELFNPTTNLELSLEYLNYISKFCAKYDPNWSN
                     SDLDAKRKKILFAYNAGVGTARKYNFNHTNKKLPRENREYPEKIMNAYYNSGIKIKT"
     gene            716..2899
                     /locus_tag="PLU55_02605"
     CDS             716..2899
                     /locus_tag="PLU55_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86978.1"
                     /translation="MKKEIVFTLLFVLVISSFASAEMIINQQPNKVHNIGDSVTIPVT
                     IKTITGVSGTFSMDLLCGTKQINFYKNGVNLVYGEEKTFDASLVLTKEVVGENVGNCK
                     IKGILGTEYFLTNEFKISNLITLKPNTEQSEFEPGQNLLIYGDAIKENGQPANGFIEL
                     ELVIANGSSNNILQKGTINNGMYSMNVVLPEKMKAGKYLVKLNAYEVALDNTKTNKGF
                     VNYNIQIKQIPTSLEIILKDKDVKPGNPVEIKTILHDQTGESIESTSVITIKTDILEI
                     PEGAGPIEKKTGEYFKFPIAYNEPPATWTVHAVSNELTAETSFTILENHEISVELVNQ
                     TLVVTNVGNIPYNATINIRIGNISKDFDLFLPVDGSNEYGLKTEHAGEYEVELMDGTR
                     TSKFKEKVVLPESRIPGSTGFAIKDFSESTTREFYTRPIFWVFVLLMIASLIFVLTRK
                     YKRRKETGVSKKSVFKKKFVAPKTNTAWENRAIQLSKESKLKINNRANLSISIKGNKQ
                     EVSMANISVRNLAELQAKKSNAEEPLQKIVNFAENKKAFVYENQNNLTFILAPSKTRT
                     LKNETAALEIAQNAKEILSNYNRIAKHKMDFGISIDYGSIVEKTEGGVMQFMGLGNFM
                     NNSKKIASCSQGEVLLSEEIRNKLTNVRTERKDNERVVCYKIKDMKYHDEGHSRYMQN
                     FMNKKTGEASGGTKYNTLPPKEKSQNTNQNTEKSTDPKSLIKGFY"
     gene            complement(2903..3769)
                     /locus_tag="PLU55_02610"
     CDS             complement(2903..3769)
                     /locus_tag="PLU55_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86979.1"
                     /translation="MSFIRGAVITLFGLVLTICILLMNVSITFASSLDYDDLKPALDN
                     SANGVIDSIVDFEEVFGKELNNYCNIDSQYIFNYGNESFAIPCEVIEEGEDSIATYIQ
                     NNFIEQIYYADYDCKFWECVKESEVPFVLISDKARVYWAGKAILLAVMSLVLFGLIFL
                     VAENRPSKFISFGILLALCSLPFKNMNWISKIVPAQVAPLFSLFSQKAHTVFVIMLII
                     SIILALIGIVALIFGWKMKFKEENLENKKSASLNQISREEIREMIREELYKKDISKKK
                     TNKSNLPKKKRN"
     gene            complement(3821..4720)
                     /locus_tag="PLU55_02615"
     CDS             complement(3821..4720)
                     /locus_tag="PLU55_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPJ86980.1"
                     /translation="MEKYQRIDFRDQISKNKRKSFFLILIVVLVIIALGYVISLAFDP
                     VWFWFIMIFSIIFSISYTLISYYNSHKISIKSVGAKKADRSKYKDFYNIVEGLTLASG
                     LPMPKLYVMPSNQINAFASGRDPKHAVICVTEGALAKLDRRELEGVLAHELGHIGNYD
                     IRYMTLVAVMVGMVAIISEMFLRSLWLKGGGDNDNNKLGIILLIIGILLAILAPIVVQ
                     LVQLAISRKHEYSADATAVKFTRYPQGLIGALKKIKQDYSYETSEKKVSKAIAPLFFA
                     SPLKNLTSTHPPIEKRIEALERM"
     gene            4850..7495
                     /locus_tag="PLU55_02620"
     CDS             4850..7495
                     /locus_tag="PLU55_02620"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPJ86981.1"
                     /translation="MTIVSEAVQYSREEIEKILHPLVREWFFSKFQDFSESQKFGVMN
                     IHNKQNILISAPTGGGKTLTAFLSILNQLVSLSEKNKLEKKVYCIYVSPLKALNNDIH
                     KNLIEPLEEINELAKSKGINLQKILVGLRTGDTTASEKAKMVKNPPHILITTPESLAI
                     VLTTKKFVESFQDVEYCILDEIHSLGNKRGSFLSLNVERLNSIAKTIPVKIGLSATIE
                     PLEEVAKYLVGIDEDRKVKIAKVHSHKKLDLDVLIPTANLILENKPHIHMYRLIHDLV
                     KEHKTTLIFTNTRSATERIVNYLKDKFPVDYTDENIAAHHSSLSKGHRFAIEENLRQG
                     NLKVVVCSTSLELGIDIGYIDLVILLGSPKASSRALQRVGRAGHKLHETAKGRFIVLD
                     RDDLIECSVIQKEMYEARIDKIHFPKNALDVLSQTIFGMAISQHWKLEDLLNLIRKSY
                     CYQKLTKEDFLSVISYLSGEYDLEKDKVYAKIWYDKDTKEIGKRGKLARVLYLTNLGT
                     IPEESFVTVKLQTGEKIGVIDESFMNRMHKGDVFVLGGSKYAYLYTKGMNLTVKPAPD
                     RNPTIPSWFSETLPLSFDSALEISKFRKEMRKRFEKNLDKEKIKEFIRKYTYTSMEVS
                     EEIYEYFYQQFHFSEIPDIDTITIEKYTDERDDKKYILVNSMYGRRVNDALSRALAYL
                     MGAKGSAKRDVEVGISDRGFYLSSENTKIEKAFEQLNSKNIEEILKQAIEKTDVLTKK
                     FRHCATRGLMILRTYKGATKSIKKQQMKSHFLISSVKKISKEFPILKEAKREVLEDLM
                     DIESTKLVLDWINSGKVKLSYNHVKLPSPFSLNLILQGHLDLMKIEDKQDFLQRMHKV
                     YIQEIARRQDPEEFSYEEFLKDQER"
     gene            7497..8240
                     /locus_tag="PLU55_02625"
     CDS             7497..8240
                     /locus_tag="PLU55_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPJ86982.1"
                     /translation="MKRENKSKDFTFISKTLFFPKKGILAIGDLHLGYDSMIKDQGIN
                     LLFNQLEETKKEMEIIIKRIKAIYRLKKIILLGDMKHHFHFQKQEVSDLRNFLRFLEK
                     HVPKEDIILIRGNHDTFTLSDYKLNDFYIEGDIAFTHGEKDFLELYDKQIKTIVTSHV
                     HPAVLICDKINIKREKYKCFLVGKYKKKQFIIVPSFFSITEGTEISEYKKGESYQQLI
                     PKNKLKSFETYVVGRDDVYHFGKYGKLIG"
     gene            complement(8265..8825)
                     /locus_tag="PLU55_02630"
     CDS             complement(8265..8825)
                     /locus_tag="PLU55_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPJ86983.1"
                     /translation="MALGAGLWILIILVAFLIIVLIGYYNRFTVLSNRIENSLGQIDV
                     QLKRRADLIPNLIETVKGFAKHEKEAIAAVTNARKALVSATDLPSRLKADKGLEGALG
                     RLFAIAEGYPDLKANTNFLELQRELTSTEDKVAYSRQFYNDSILDYNNKCKTFPGKVF
                     AGIFGFKEKEYIKITEEERKPVKVKF"
     gene            8969..9151
                     /locus_tag="PLU55_02635"
     CDS             8969..9151
                     /locus_tag="PLU55_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004801640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPJ86984.1"
                     /translation="MVKVNKNKCIGCGACAATYSEIFHMEDDGKAGVKSGVDAKKNAK
                     SIKEAIKNCPAEAISN"
     gene            9193..11280
                     /locus_tag="PLU55_02640"
     CDS             9193..11280
                     /locus_tag="PLU55_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPJ86985.1"
                     /translation="MNQREATNNSSDERSELVKWFSDLGKDSENLVGEKGANLSEIYN
                     LKLPITPGFIVTTKAYDYFLENSPGLNNKIKGLLEKINYEDPILLNDIAEVIRNGIME
                     AHMPQILEEEILDAYDSLGAGDLSDIYGSALDILQNAQEPIFVAVRSSAPAGNNPKEG
                     LVVQQDSFLNVKGESELITHIKKCFASLFTPNEIYARYKKGLKDSPIKLAVIVQKMVD
                     SNKSGVIFSKNPNNLDENMIIQAIWGLGEGASSKIITPDEYVLNKNLEIIDIKVAKKE
                     IAITRDSSGSRITIPLREEKSKQQVLTKRELTRLAKLSLEIEEHYKRPQCIEFAIEGE
                     DIYLVQTREMKTNHSNKEIKIEEDIKREIKEPVEPEKIQEIKPVATKTKTKVKLIINS
                     PSFVDRAIHTGIKSAGIIKIEDIILESGKHPKYYLTKNAVKEYEDIIFKELNKIAEHF
                     NELWIRTSDIIGDEFPELEGTPNEKEANPRLGLHGIRFSLNNPEIFEAELNAFKRVSE
                     SGKEVGVIIPNLVQIEELRKVKEILDKINFKEVRVGVSIETPASVQLINQICDERINF
                     ISIDINNLTQNILSIDKRNKNVLYLYNELHPAILYQLSYIIRVAKKKGVKTSIILESE
                     KKEELIPFLIKEGIDIICMKAEDTEKIVNCIKEVEENIIKGTDQEPRKYTPKENKSEE
                     TPVIKGIEKLHER"
BASE COUNT         4641 a         1477 c         1748 g         3422 t
ORIGIN      
        1 actccttttg aaaaagcaaa agaacaacat gaactttata aaaaatcaga acttgaaaag
       61 acattaaaag aagaaaaaag acaaaaagaa gaagcagaaa aaagaaaatt tttagaacct
      121 tactttcttt ctcaagattc tttaaattca tatattaagc aagcatactc aaatgttaaa
      181 aaatggccaa atgagtttga taaaaggctt tttagggtta tgttaagaca agaatcttca
      241 tataatgtac atgcagtaag taaatcagga tataaaggat taggtcagat aggtccagaa
      301 gtttatcaaa catttagacc agaaaaattt gaagcactta aggattcaat aacaggacag
      361 ataaatgaat atgcactaca aaaagaatta tttaatccta ctacaaatct tgaactttca
      421 cttgaatatt taaattatat ttcaaaattc tgtgcaaaat atgatccaaa ctggtcaaat
      481 tcagatttag atgcaaaaag aaaaaagatt ctttttgcat acaatgcagg agtaggtaca
      541 gctagaaaat ataatttcaa ccatactaac aaaaaacttc caagagaaaa cagggaatat
      601 cctgaaaaaa taatgaatgc ttattataat tcaggtataa agattaaaac ataactttta
      661 attaataatt ctcatctcca caaatattta taaataaata tttgttagta tataaatgaa
      721 aaaagagatt gtgtttacat tattgtttgt attagtaatt agttcttttg cttctgcaga
      781 gatgattata aaccaacaac caaacaaagt acataatata ggagattcag taacaatacc
      841 tgtaacaata aaaactatta caggagtttc tggaacattt agtatggatt tgctttgtgg
      901 aacaaaacaa attaattttt ataaaaatgg agtaaatctt gtatatggag aagaaaaaac
      961 atttgatgct tctcttgttt taacaaaaga ggttgttgga gaaaatgtag gaaactgtaa
     1021 aataaaagga atacttggaa cagaatactt tttaacaaat gaatttaaga tatctaattt
     1081 aattacactt aagccaaata ctgaacaaag tgagtttgaa cctggtcaaa acttattaat
     1141 atatggagat gcaataaaag aaaacggaca accagctaat ggatttatag aactggaatt
     1201 agtaattgca aatggttcat ctaataatat cttacaaaaa ggtacaataa acaatggaat
     1261 gtattcaatg aatgttgttc ttccagaaaa aatgaaagct ggaaagtatt tggttaaatt
     1321 aaatgcttat gaagttgcat tagataacac aaagacaaac aagggatttg taaattataa
     1381 tatacaaata aaacaaattc ctacttcttt ggagataatc ctaaaagata aagatgttaa
     1441 acctggaaat ccagttgaaa taaaaacaat cttacatgat cagacaggag agagtataga
     1501 atcaacttct gttatcacaa taaaaacaga tatacttgaa attccagaag gggcaggacc
     1561 aattgagaag aaaactggtg aatactttaa atttccaata gcatataatg aacctcctgc
     1621 aacctggaca gtgcatgctg tatctaatga attaacagca gaaacttctt ttacaatatt
     1681 agagaaccat gaaataagtg tagaacttgt gaatcaaact ttagttgtaa ctaatgttgg
     1741 aaatatccca tataatgcta caataaatat aaggatagga aatatctcta aggattttga
     1801 tttattcttg cctgttgatg gtagtaatga gtatggatta aaaacagagc atgctggaga
     1861 atatgaagta gaattaatgg atggaacaag aacaagtaaa ttcaaagaaa aagtagtttt
     1921 acctgaatca agaatacctg gatcaacagg atttgcgata aaagattttt cagaatctac
     1981 tactagagaa ttttacacaa gacctatttt ttgggtgttt gtattgctga tgatagcatc
     2041 attaattttt gttttgacaa gaaaatataa aagaagaaaa gaaacaggag tatctaaaaa
     2101 aagtgtattc aagaaaaagt ttgtagcacc aaaaacaaac actgcttggg aaaatagggc
     2161 aatacaatta tcaaaagaat ctaagcttaa aataaacaat agggcaaacc tttctatttc
     2221 aataaaagga aataagcaag aggtaagtat ggcaaatata agtgtaagga atttagctga
     2281 gcttcaagca aaaaaatcaa atgcagaaga gcctcttcaa aaaatagtaa atttcgcaga
     2341 aaacaaaaag gcatttgtat atgaaaacca aaacaactta acatttatat tagctccttc
     2401 aaaaacaagg acattaaaaa atgaaacagc tgctcttgaa attgcacaaa atgcaaaaga
     2461 aatcttatct aattataaca gaatagcaaa acataagatg gattttggaa tatcaataga
     2521 ttatggttca atagttgaaa aaacagaagg tggagtaatg caatttatgg gattagggaa
     2581 ctttatgaat aattctaaaa agatagcatc ttgttctcag ggggaagttt tattgagtga
     2641 agaaataaga aataaattaa caaatgtaag aacagaaaga aaagataatg aaagagtagt
     2701 atgttacaaa attaaagaca tgaaatacca tgatgaagga cattcaagat acatgcaaaa
     2761 ttttatgaac aaaaaaacag gggaagcaag tggaggaaca aaatataata cattacctcc
     2821 taaagaaaaa tcacagaata caaatcaaaa cactgaaaaa agtacagacc ctaaatcttt
     2881 gattaaagga ttttattaaa aattaattcc tctttttctt tggtaaatta gatttgttgg
     2941 tttttttctt tgaaatatct tttttatata attcctctct aatcatctct ctaatttcct
     3001 ctctggaaat ttgatttaaa gaagcagatt ttttattctc taaattttct tctttgaatt
     3061 tcattttcca tccaaatatt aacgcaacta ttcctattaa tgcaaggata attgagataa
     3121 ttaacattat tacaaatact gtgtgagctt tctgtgaaaa gagtgagaat aaaggagcaa
     3181 cctgagctgg aactatcttt gaaatccaat tcatattttt aaaaggtaat gaacaaagag
     3241 ctaaaagaat cccaaaagaa atgaacttgg atggtctatt ttcagcaact aaaaaaatca
     3301 aaccaaataa aaccaaagac attacagcta ataaaatagc tttacctgcc caatataccc
     3361 ttgctttatc agaaataaga acaaaaggaa cttcagattc tttaacacat tcccagaatt
     3421 tacagtcata atctgcataa taaatttgtt caatgaaatt attttgaata taagttgcta
     3481 tagaatcttc cccttcttca ataacttcac aaggtattgc aaagctttca tttccataat
     3541 taaaaatgta ctggctatca atattacaat aattgtttaa ttcttttcca aaaacttcct
     3601 caaaatctac aatactatca ataaccccat ttgctgaatt atctaaagca ggtttaaggt
     3661 catcataatc aagagaagaa gcaaatgtta tggaaacatt cattaaaaga atacaaattg
     3721 tcaaaacaag accaaatagt gtaattactg cacctcttat aaagctcata attaatccaa
     3781 taaataaagg tttttaaaac taattaaaac agctttgaaa ttacattctt tctaaagctt
     3841 ctattctctt ctcgattgga ggatgtgtgc ttgtaagatt ctttaaaggg ctagcaaaaa
     3901 acaatggagc aattgcttta ctcactttct tttcagaagt ttcataggag taatcttgtt
     3961 ttattttttt caaagctcca attaaccctt gaggatatct tgtgaattta actgcagttg
     4021 catctgcaga atattcatgt tttcttgaaa ttgcaagttg aactaactga acaacaatag
     4081 gagcaagaat tgctaaaaga atacctatta tcaagaggat tatgcctaat ttattattat
     4141 cattatctcc tccccctttt aaccataaac ttcttaaaaa catttcagag ataattgcaa
     4201 ccattccaac cataactgca actaatgtca tatatcttat atcgtaattt ccaatgtgtc
     4261 ctaattcatg tgctaaaact ccttctaatt ctcttctatc tagtttagcc aaagctcctt
     4321 cagtaacaca aataacagca tgtttaggat ctcttccaga agcaaaggca tttatttgat
     4381 tagagggcat aacataaagt ttaggcatag gaagtccaga agctagagtt aaaccttcaa
     4441 caatattgta aaaatcttta tacttgcttc tgtctgcttt ttttgctcca acactcttaa
     4501 ttgaaatttt atgtgaatta tagtaactaa taagagtata ggaaattgag aaaataattg
     4561 aaaatatcat tataaaccaa aaccaaacag gatcaaaagc tagggaaata acatacccta
     4621 atgctataat cactaaaaca acaattaata ttagaaaaaa tgattttctt ttattttttg
     4681 aaatctggtc tctaaaatca attctttgat atttttccat atatagatat agaaaaatgg
     4741 ctttttaaga ttaaccaaaa taaccaaaac ccttaaataa tgacacttaa ctaatttaaa
     4801 ttagagattg aagaggtttc tatagaattg aatgtaaaag gtaacttaaa tgacaattgt
     4861 ttcagaagca gtccaatatt ccagagaaga aatagaaaaa atactccatc cacttgtaag
     4921 agaatggttt ttctcaaaat tccaggattt ttcagaatct caaaaatttg gagtaatgaa
     4981 catccataat aaacagaata tacttatatc tgcacctaca ggaggaggta aaactctaac
     5041 agcatttctc tcaatattga atcagttggt atctttgtca gaaaaaaata aacttgaaaa
     5101 gaaagtttat tgcatctatg tttctccttt aaaagctctt aataatgata tacacaaaaa
     5161 tcttatagaa cctcttgaag aaataaatga acttgcaaaa tcaaagggaa taaatctcca
     5221 gaaaatactt gttggtttaa gaacaggaga tacaactgct tctgaaaaag caaaaatggt
     5281 aaaaaaccct ccccacattc ttataacaac tcctgaatct cttgcaattg ttttaacaac
     5341 aaagaaattt gtagaatctt ttcaggatgt agaatattgt attcttgatg aaatacactc
     5401 tttaggaaat aaaagaggtt cttttttaag tttaaatgtt gaaaggctca actcaattgc
     5461 aaaaacaatt cctgttaaaa taggtttaag tgcaacaata gaacccttag aagaagttgc
     5521 aaaatatttg gttggaatag atgaagacag aaaagtaaaa attgcaaaag ttcattccca
     5581 taaaaaactt gatttagatg ttttaatacc cacagcaaat ttaatcttgg aaaataaacc
     5641 acacattcat atgtatcgtt taatacatga tttagttaag gaacataaaa caactttaat
     5701 ttttacaaat actcgttcag caacagaaag aattgtaaat tatctgaaag ataaatttcc
     5761 tgtagattat accgatgaaa atattgcagc acatcattct tctttaagta aaggacatag
     5821 atttgcaatt gaagaaaatc tcaggcaagg aaacttgaaa gtagttgtat gctcaacttc
     5881 tttggaatta ggaatagata ttggttatat tgatttagtt attcttttag gtagtccaaa
     5941 agcttcttca agagctcttc agagagtagg aagagcagga cataaattac acgagacagc
     6001 aaaaggaaga tttattgtat tagatagaga tgatttaatt gaatgttctg taattcaaaa
     6061 agaaatgtat gaggctagaa tagataaaat acattttcca aaaaatgctt tggatgttct
     6121 ttcccaaaca atttttggga tggcaataag tcaacattgg aaattagaag accttcttaa
     6181 tctaataaga aaaagttatt gttatcaaaa gttaactaaa gaagattttc tttctgtaat
     6241 aagttattta tctggagaat atgatttaga aaaagacaaa gtttatgcaa aaatttggta
     6301 tgataaagat acaaaagaaa taggtaaaag aggtaaatta gcaagagtat tatatttaac
     6361 aaatcttgga acaattccag aagaatcttt tgttactgta aaacttcaga caggagagaa
     6421 gataggtgtt attgatgaat cttttatgaa tagaatgcat aagggagatg tttttgtttt
     6481 aggtggaagt aaatatgctt atttatacac aaaagggatg aacttaacag ttaaacctgc
     6541 tccagataga aaccctacaa taccttcttg gttttcagag actcttccat taagttttga
     6601 ttctgctttg gaaatatcta aatttagaaa agaaatgaga aaaaggtttg aaaaaaatct
     6661 ggataaagaa aaaataaagg aatttataag aaaatatact tatacttcaa tggaggtctc
     6721 agaagagatt tatgaatatt tttatcaaca attccatttt tctgaaatac cagatataga
     6781 tacaataaca attgaaaaat atacagatga aagagatgat aaaaaatata ttcttgtaaa
     6841 ctcgatgtat gggagaagag taaatgatgc tctctctcgc gctttagcat atttaatggg
     6901 tgctaaaggt tctgcaaaaa gagatgttga agtaggaata tctgatagag gattttatct
     6961 ttcttctgaa aacaccaaaa tagaaaaagc atttgaacag cttaattcca agaatataga
     7021 agagatatta aaacaagcta ttgaaaagac agatgttctt acaaagaagt tcaggcactg
     7081 tgcaacaaga ggtttgatga ttttaagaac atataagggt gctactaaat ctattaaaaa
     7141 acaacaaatg aaatcccact tcttgataag ttcagttaaa aaaatatcca aagaatttcc
     7201 aatattaaaa gaagcaaaaa gagaagtcct agaagattta atggatattg aaagtactaa
     7261 attagttctt gattggataa attcaggaaa agtaaaatta agttataatc atgtaaaatt
     7321 gccttctcca ttttcactaa atctcatact tcaaggacat cttgatttaa tgaagataga
     7381 agataaacaa gattttcttc aaagaatgca taaggtatat attcaggaaa tagctcgtag
     7441 acaagacccg gaagaattta gttatgagga atttttaaaa gaccaggaga gataaaatga
     7501 aaagagaaaa taaatcaaaa gattttacat tcatctcaaa aacacttttc tttcctaaaa
     7561 aagggattct cgcaatagga gatttacact taggatatga ttcaatgata aaagatcagg
     7621 gaataaatct tttatttaat caattagaag aaacaaaaaa ggaaatggaa attataataa
     7681 aaagaataaa ggcaatttat agattaaaaa agataatcct tcttggagat atgaaacacc
     7741 actttcattt tcaaaaacag gaagtatcag acttaagaaa ctttttgaga tttctagaaa
     7801 aacatgttcc aaaagaggat ataattctta tacgaggaaa tcatgatact tttacattaa
     7861 gtgattacaa actaaatgat ttttatatag aaggggatat tgcatttacc catggagaaa
     7921 aagactttct ggaattatat gataaacaga taaaaacaat agttacaagc catgttcatc
     7981 cagctgtttt aatctgtgat aaaattaata taaaacgtga aaaatataag tgctttcttg
     8041 ttggaaaata taaaaaaaag caatttataa tagttccaag ttttttcagt attactgaag
     8101 gaactgaaat atctgaatat aaaaaaggag aatcttatca acaactaata cctaaaaata
     8161 agttaaaatc ctttgagaca tatgttgttg gacgtgatga tgtttatcat tttggaaagt
     8221 atggtaaact aataggttaa tcaacaatag aaaataaact atatttaaaa cttaacttta
     8281 acaggttttc tttcttcttc agttatctta atgtattctt tttctttgaa tccaaagatt
     8341 cctgcaaata cttttcctgg gaaagtctta catttattat tgtaatcaag aattgagtcg
     8401 ttgtagaatt gtcttgaata agcaacttta tcttctgttg aagttaattc tctctgtaat
     8461 tctaagaaat tagtattagc ctttaaatca gggtatcctt cagcaattgc aaataatctt
     8521 cctaatgctc cttcaagtcc tttatctgct tttaatcttg aaggtaaatc tgttgcacta
     8581 accaatgctt ttcttgcatt tgtaactgct gctattgcct ctttttcgtg tttagcaaaa
     8641 cctttaacag tttcaataag gtttgggatt aaatctgctc tccttttcaa ttgaacatca
     8701 atctgtccaa gtgaattttc tattctatta gaaaggactg taaatctgtt ataatatcca
     8761 ataagaacaa ttattaagaa tgcaactaat ataattaaaa tccataatcc tgctcctagt
     8821 gccataaata aatatgaata tttatcttta taaatttaac taatgggtta aagttaaagt
     8881 tagttttttg acagaaacta ctttttaaac aaagccagtt tcaacattat atttataaac
     8941 ttgcctaaat ttcaccaagt aaaataaaat ggtgaaagtg aataaaaata aatgcattgg
     9001 gtgcggagca tgtgctgcaa catattctga aatttttcac atggaagatg atggaaaagc
     9061 aggtgtaaaa tcaggagtag atgctaaaaa aaatgcaaag tccataaaag aggcaattaa
     9121 aaactgccca gcagaagcaa tatctaacta aaaaccccaa acatttatta atataaatta
     9181 tttagtgaaa ttatgaatca aagagaggca acaaacaact cttctgatga aaggtctgag
     9241 ttggtgaagt ggttttctga tttaggaaaa gattctgaga atttggttgg tgaaaaagga
     9301 gctaatttga gtgaaatata taatttaaaa ttacctataa ctcctggatt tattgtaaca
     9361 acgaaagctt atgattattt tttggaaaat tctcctggct taaataataa aataaaagga
     9421 ttacttgaaa agataaatta tgaagatcca atccttttga atgatattgc tgaagttata
     9481 agaaatggta taatggaagc acatatgcct caaattttag aagaagagat attagatgca
     9541 tatgatagtt taggagcagg tgatttaagt gatatttatg gttctgcatt agatattctg
     9601 caaaacgcac aagaacctat ttttgttgct gttcgttctt ctgcaccagc tggaaacaat
     9661 cccaaagagg ggttagttgt tcagcaagat tcatttttga atgttaaagg agaaagtgaa
     9721 ctaattacac acataaaaaa atgctttgca tctttattca ctcctaatga aatttatgca
     9781 agatataaaa agggtttaaa ggattctcca ataaaactcg cagtaattgt tcaaaagatg
     9841 gtagattcta ataaatctgg agtaattttt agtaagaacc caaataattt agacgagaac
     9901 atgataattc aagcaatatg gggtcttgga gaaggtgcat cttcaaagat aataactcct
     9961 gatgaatatg tgctcaataa aaatctagaa ataattgata taaaagttgc aaaaaaggaa
    10021 atagcaatta caagagattc ttcaggaagt aggataacaa tacccctaag agaagaaaag
    10081 tcaaaacaac aggttttaac aaaaagagaa ttaacaagat tagctaaatt atctttagaa
    10141 attgaagaac attataagag accacaatgt attgaatttg caattgaagg tgaagatatt
    10201 tatttagttc aaacaagaga gatgaaaaca aatcattcaa ataaagaaat caaaatagag
    10261 gaagatataa aaagagaaat aaaagaacct gtagaaccag agaaaataca agagataaaa
    10321 ccagtagcaa caaaaacaaa aacaaaagta aaacttataa tcaattctcc ttcttttgta
    10381 gatagagcaa tacacacagg aattaaatct gctgggataa ttaaaataga agacataatt
    10441 ttggaatctg gaaagcatcc aaagtattat ctaacaaaaa atgcagtaaa agaatatgaa
    10501 gatataattt tcaaagaatt aaataagata gcagaacatt ttaatgaatt gtggataaga
    10561 acatcagata ttataggtga tgaatttcca gaattagaag gcacacctaa tgaaaaagaa
    10621 gctaatccta gattggggtt gcatggaata aggtttagtt taaataatcc agaaatattt
    10681 gaagcagaat tgaatgcatt taaaagagta agtgaatcag gcaaggaagt tggagtaata
    10741 atacctaatt tagttcagat agaagaattg cgaaaagtaa aagagatatt agataaaata
    10801 aactttaaag aagtgagggt tggagtctcc atagaaactc ctgcatctgt acaattaata
    10861 aatcaaatat gtgatgaaag aataaacttt atttcaatag atataaacaa tttaacacaa
    10921 aatattcttt ctatagataa aagaaacaag aatgttttat atttgtataa tgagttgcat
    10981 ccagctatac tttatcaatt atcttatatt ataagagtag ctaagaaaaa aggagtaaaa
    11041 actagtataa ttcttgaatc agaaaaaaaa gaagaattaa ttcctttctt aataaaagag
    11101 ggaattgata taatatgtat gaaagcagaa gatactgaaa aaatagtaaa ctgcataaaa
    11161 gaggtagaag aaaatataat aaaaggaaca gaccaagaac caagaaagta tactccaaaa
    11221 gagaataaat cagaagaaac acctgtaata aaaggaatag aaaagctgca tgagaggtaa
    11281 aaaagtaa
//
LOCUS       DAOPCH010000024        10938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_5859_length_10938_cov_4.248461, whole genome shotgun sequence.
ACCESSION   DAOPCH010000024 DAOPCH010000000
VERSION     DAOPCH010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 10938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10938
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5859_length_10938_cov_4.248461"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLU55_02645"
     CDS             complement(<1..695)
                     /locus_tag="PLU55_02645"
                     /inference="COORDINATES: protein motif:HMM:NF022932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPJ86986.1"
                     /translation="MIVKDEFLSRLRKIFDLNLYEVKVWTALLSRGTSTAGELSNISD
                     VPRSRTYDILESLEKKGFIVMKLGKPIKFVALKPEEVVERVKKNLIKYAQERTKRLET
                     LKDDEVLEELNGLFTKGIKFVEPSELSGSIKGRQNLYNHLDMMIRDAEKTITIVTTAE
                     GLNRKLEALMPILEKIKKRGVKVRIASPINNNNIKYARELKKVAEVKEMEGIRARFVI
                     VDGNQIMFMLLDD"
     gene            complement(778..3576)
                     /gene="ileS"
                     /locus_tag="PLU55_02650"
     CDS             complement(778..3576)
                     /gene="ileS"
                     /locus_tag="PLU55_02650"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPJ86987.1"
                     /translation="MYDFKDVEKVAREIWKKHKKELKESVQDNPKKPLFSFLEGPPTA
                     NAPPGLHHLEARTFKDLICKFRYMNGFSVPRKGGWDCHGLPVEVQVEKELKLESKKEV
                     EKYGVDKFIEKCRESVFSNIEDWNKSTEELNYLIDLENPYITLENNYIESVWWSLKEL
                     YKKKLLYEGYKVLPFCTRCGTPLSSHEVAQGYKEVQEESVYIAFRIKGKKDEYILAWT
                     TTPWTLPGNVALAVGPKIEYVKVELPDGDKLIIGKEKIGLLKGQCKIIEKLKGKDLIG
                     LEYEPLYEIKELKSDNSYRIVAADFVTTEDGTGVVHTAVMYGEEDYNLGKEIGLPEVH
                     TVGQDGKFLDIVPEFIRGKYVKSAEKDIKEDLKKRHLLFKVEKISHDYPFCWRCDSPL
                     LYYGINSWFINASETRKRLVELNKEINWYPSHIKEGRFGKWLDGAKDWALSRFKFWGT
                     PLPIWKCECGKEKIIGSIEELKKEAIKMPKEKIDLHKPWIDKVKLRCSCGKEMRRIPD
                     VIDCWYDSGSASFAQFHYPFENKKEFERRYSYDYIAEGIDQTRGWFYTLHFLGTVLFD
                     KPAYKNAICMGHIVDEKSEKMSKSKGNIIKPREIIDKAGVDAVRLQFLTSDVGNQKRF
                     SYELMKENVLGFLNVLYNCNTYYKQLDNKKCSYKIEDKWILSKLNNLVKEVTADLNNY
                     ELNKPFEKINNFVVNNFSRGYIKMTRDREDTKEILREVLEKISLLLAPFAPHISEYIY
                     SGFSKNSVHLSKWPKFDESKINLELEKEMELTMKVIEIGLAERDKAQIGLKWPLQRAT
                     ICGKYLDFEEELIEILKSQLNVKEIVIEEEEELKVVLDREITPELESEGYAREIARQV
                     QEFRKKLGLSKKENIELYIATSDEFKEIIRSKTDFIKLRTNSKKLEFVATLQKEKFKN
                     TVSFNIKDKRGEIGIVIK"
     gene            complement(3631..4542)
                     /locus_tag="PLU55_02655"
     CDS             complement(3631..4542)
                     /locus_tag="PLU55_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPJ86988.1"
                     /translation="MNKMKILGLIFVFLSMILSLANIFSVKITGAIIGIGVKSSLITI
                     FTLVFLFLGIFFLGNLEKKVSNWVKAAAIAGSIGLIAAPAYHARKDKLKKRTKENVEI
                     NKGVKVVSPYMTTQGRFERTYRWDKILDEIEDKYELPRGILKGLVMRESYGDPLRLNA
                     MGDGGAGLFMFQPRTAKAYGLKVHGTSDRSSADKAHGAELKELVKKHNYDYGKLAVID
                     ERFDVRKSAEAAAKYLRDDFKRYGSWDKALSAYNQGKPAPNASETKHVKAVREYQAYY
                     NERDKKDHKYSTQKTNAKSKAKRQRKR"
     gene            4632..4958
                     /locus_tag="PLU55_02660"
     CDS             4632..4958
                     /locus_tag="PLU55_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86989.1"
                     /translation="MDNYLLPRLDSLSDEERHLFYKRLTDILNDRYSCFVYGGMNNFE
                     YATMPKNKLERIKINLYNHMFENINPPMRVITDFLSPNKVKEKLDINLSKLSTLNIDD
                     TVLSWL"
     gene            complement(4977..5693)
                     /gene="dph5"
                     /locus_tag="PLU55_02665"
     CDS             complement(4977..5693)
                     /gene="dph5"
                     /locus_tag="PLU55_02665"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HPJ86990.1"
                     /translation="MLYLIGLGLNEKSLSKEAIEIIKRCKKVYLEDYTVDFPYTHQAL
                     IDEIGKKFVTADREKVESLEIVDEAKKMDVALLVYGSPLTATTHITLIEEAILSGIKY
                     RIINNASVLDGIAETGLQVYKFGKITSLPEWKKSYEPDSFMEIVKENQSIKAHTLLLV
                     DIGLDIKKAIEQLEKAAKKHNFKLFKLIICQALGTKKQKIIYKDLEKIKEFTNVQKPY
                     CIIIPGELHFMEKKMLEEFS"
     gene            complement(5758..6030)
                     /locus_tag="PLU55_02670"
     CDS             complement(5758..6030)
                     /locus_tag="PLU55_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86991.1"
                     /translation="MKNKITPKSTREEVINPELDFSNDSGIKCKNAPPKREPAESETR
                     NKIILLSLSFFNKTKNIPTREINDTIITLRIEYTSGFIEIIERKGF"
     gene            6020..6538
                     /locus_tag="PLU55_02675"
     CDS             6020..6538
                     /locus_tag="PLU55_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPJ86992.1"
                     /translation="MFFIIEHAIQWRHCHIPTSKSHPHSLGIMNIIGDGFHNFLDGLV
                     IAAAYFINIPLGIVTTLAVILHEIPQEIGDFGVLIYAGYSKKKALFFNFLSALTAVLG
                     AIVGILFAGSSDMFALIAIPIAAGGFLYIAGSDLIPELHKNQEKHFSINKIIGIILGI
                     LLMYGLTFLKVA"
     gene            6599..7003
                     /locus_tag="PLU55_02680"
     CDS             6599..7003
                     /locus_tag="PLU55_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86993.1"
                     /translation="MVEIGKVLELFNKTNPSNVEKIWKGFMIKKKDFYFYSHNLFSEM
                     YELTIFEQGNIVPLQKFVYNERNKEGDKKVEECYNLLHRKYFEWKKHEEEVMIFEAQE
                     QIEKNKREEEMKDVLNEAERIQSFKKLEKILS"
     gene            complement(7009..7617)
                     /locus_tag="PLU55_02685"
     CDS             complement(7009..7617)
                     /locus_tag="PLU55_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ86994.1"
                     /translation="MVKEPYYKSRKGNEEFSKRKHMASLAKYNPLSAYGIARKIDTPS
                     SSRGDSVEIREEKYTFGLASMDEKIRSGDLREAYFTFLNLQKRLDQEQIMPKDKVEKY
                     QEALARRGSRLIAKSDKSENYFEDNKVILKYCKKHLKKNKSSGLEKAITIISLGSMIL
                     GVAIGYPALTGNAIADVVSGSVIHGAALFFLGLLGVFLANKK"
     gene            complement(7675..8484)
                     /locus_tag="PLU55_02690"
     CDS             complement(7675..8484)
                     /locus_tag="PLU55_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPJ86995.1"
                     /translation="MGSIKIGPTGLGSVRESEKTLQEFSKNGFNNCEVLFVQQVYIDK
                     EEAKEIKEFAKKNKIDLSVHAQYFINLNSEEKEKVHASMQRILKCCEIGHELGAKSIV
                     FHPGFYGKEFQKAKTLEDKKRVEEETFQNIKKRVLEIMETIEKKKWDVELCPETMGKV
                     NVFGSPEEISRLVKETGCSFCIDFAHILAREKKVDYEKIKRLFPQKKWHCHFSGIEYG
                     DKGERNHIRVEKKQWKELFENLPKDKDITIVCESPYSVEDCIEGLKVLNGK"
     gene            complement(8520..>10938)
                     /locus_tag="PLU55_02695"
     CDS             complement(8520..>10938)
                     /locus_tag="PLU55_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HPJ86996.1"
                     /translation="DKITEQAVIKWQAENGLASDGKFGKNSLEKANYVYNFDLSKSSN
                     GKSFKSGDKGEDVKSIQSMLISQGYLSKTFTNAYGKEVNSVDGYFGFRTENALKEWQK
                     DNQLTPDGQFGKLSVEKATKLSKETINLNMMGGSSTITNGKNGLDITMKGRGSLEIAG
                     KKYLFEGGEFKENLRTSLESTSVSTPITIKYIDNEGKELKLDLISPNNKIGSVELARG
                     AKILGLDVNKVVCSGGAQVGCLLVEEEYGGGIKLTNQGDAQKYLRENGVHGDAWEIQY
                     NMLKQGGGIVYSKEHELTTAERTQLKEIETELKKEYNRLKKLRLSDEVIFNKISTTNS
                     DSINKMQSILSKDYIPQQVTPQKGDIVSFYYPSSHYLTQATIEGKNSQKNTHIGVIVG
                     SNEQSLIIKGESESVFTELKGKLRVKNDDSMYVNTYNYIDEETGYVKPLTLKNKKFYT
                     EDGKELSFDSGKEILISTPVVSDFIGGQFNVRELDVLLDQPSYSLYGVTRPKSSQYLN
                     ELFVTPDIVTTYQNHIDCNAISCTGFNLEELLRANNVKKYTDWNQALLKSSTERQKEY
                     NIPDSKFNEFNALTATIITKESNWGDSKWYAAESTLARTRLDKAVSKNPTLGYMQINT
                     ADIEKYAKEFNEKTPSTIEMYRDKEVSIKYGQRYLAELVNIYSPTPGGMTENQVKSIA
                     AAYNFGKYTPRNAAVQAQLKELGYYNAKADGLWGGETEKAIINFARERKLKPISSGTF
                     YSKSFEESDIYNYIKEEYLKKTNKNPEYSLVPDWIGSTTKTKAHSVPAYANSAYEIYT
                     AYCPTCT"
BASE COUNT         3310 a         1959 c         1340 g         4329 t
ORIGIN      
        1 tcatcatcca aaagcataaa cattatttga tttccatcaa caattacgaa tctagctctt
       61 atcccttcca tttctttcac ttcagcaact ttctttaatt ctcttgcata ttttatatta
      121 ttattattta taggagaagc aattcttact ttcacccctc tttttttaat tttttcaaga
      181 ataggcatca aagcctcaag ttttctgtta agaccctctg ctgttgtaac aatcgtgatt
      241 gtcttttcag catcacgaat catcatatct aagtggttgt ataagttctg tcttccctta
      301 atacttccag ataattcact tggttcaaca aacttaatac ctttagtaaa taatccattt
      361 agttcttcaa gaacttcatc atctttaagt gtttcaagtc tttttgttct ttcttgagca
      421 tattttataa ggtttttctt aactctttca acaacttctt caggtttaag tgcaacaaac
      481 ttgataggtt taccaagttt cataacaata aatccttttt tttcaagact ttctaaaata
      541 tcatatgttc ttgaacgagg tacatctgaa atattactta gttcccctgc tgttgaggtt
      601 cccctcgaca aaagagctgt ccagaccttt acctcatata ggttcaaatc aaaaatcttt
      661 ctcaacctac ttaggaattc gtcttttact atcattttgt atatattggt ttagcgacat
      721 ctccttttaa actttgttat aataaaagag taaatttttt ggattaccgg taggtagtca
      781 ctttatgact atccctattt ctcctctttt atctttaata ttaaaagata cagtgttttt
      841 aaatttttct ttctgtaggg ttgcaacaaa ttccagtttt tttgagtttg ttctaagttt
      901 aataaaatca gttttactcc taattatttc tttgaattcg tcgctggttg caatataaag
      961 ttcaatattt tcttttttgc tcaatcctag tttctttctg aattcttgaa cctgtcttgc
     1021 aatttcacgc gcataacctt ctgattccaa ttcaggagtt atctctctgt caagcactac
     1081 ttttagttcc tcctcttctt caatgacaat ttcttttaca tttaattgtg attttaatat
     1141 ttcaattagt tcttcttcaa aatcaagata ttttccacaa attgtagccc tttgcaatgg
     1201 ccattttaat ccaatttgag ctttatctct ttcagctaaa ccaatttcaa taaccttcat
     1261 tgttaattcc atttcttttt ccaactctaa atttatttta ctttcatcaa attttggcca
     1321 ttttgagaga tgaacagagt ttttagagaa accactataa atatattcag agatatgagg
     1381 agcaaaaggc gcaagcaata atgaaatttt ttcaagaact tcccttaaaa tctcttttgt
     1441 atcttcccta tcccttgtca tcttaatata tcctcttgaa aaattgttta caacaaaatt
     1501 atttatcttt tcaaaaggct tgtttaattc ataattattt aaatcagctg taacttcttt
     1561 cacaagattg tttagcttgc ttaatatcca tttatcttca atcttataag agcatttttt
     1621 attatctagt tgtttgtagt aggtattaca gttatataat acattcaaaa aacccaaaac
     1681 attctctttc attaattcat aagaaaatct tttttgattt ccaacatctg aagttaaaaa
     1741 ttgcaatctt acagcatcaa cacccgcttt atctattatt tctcttggtt ttatgatgtt
     1801 tcctttggat tttgacatct tttcactttt ctcatcaaca atatgcccca tacaaattgc
     1861 attcttataa gcaggtttat caaatagtac tgtacctagg aaatgaagag tataaaacca
     1921 accccttgtc tggtcaattc cttctgcaat gtaatcataa ctatatcttc tttcaaattc
     1981 ttttttgttt tcaaatggat aatggaattg tgcaaacgac gcacttccag aatcatacca
     2041 acagtcaatt acatctggta ttcttctcat ctctttcccg caagaacacc ttaattttac
     2101 tttatcaatc caaggtttat gcaaatctat tttttcctta ggcattttta ttgcttcttt
     2161 ctttagttcc tcaatacttc caattatttt ttctttacca cattcacatt tccaaattgg
     2221 tagaggtgtt ccccagaatt tgaatcttga aagcgcccag tctttagcac catcaagcca
     2281 tttcccaaat cttccttctt taatgtgtga aggataccag tttatttctt tgtttaattc
     2341 aaccaatctc tttcttgttt cacttgcatt gataaaccaa gaatttattc cataatacaa
     2401 caaaggagaa tcacacctcc aacagaatgg gtaatcatgg cttatttttt caaccttaaa
     2461 taaaagatgc cttttcttta aatcttcttt aatatctttt tcagcagatt taacatactt
     2521 acctcttatg aactcaggaa caatatctaa aaattttcca tcttgtccaa ctgtatgaac
     2581 ctcgggtaat ccaatttctt tacctaaatt ataatcttct tcaccataca tcacagcagt
     2641 atgcaccaca cctgtaccat cctcagttgt aacaaagtct gcagccacaa tcctgtaaga
     2701 attatcggac ttcaattctt ttatctcata taatggttcg tattctaatc caattaaatc
     2761 ttttccttta agcttctcaa ttattttaca ctgtcctttc aagagcccta ttttttcttt
     2821 tccaattatt aatttatctc catcaggtaa ttcaacttta acatattcta ttttaggtcc
     2881 aacagctaat gcaacatttc ctggtaatgt ccaaggagtt gttgtccatg ctaaaatata
     2941 ttcatctttt tttcccttta ttctaaatgc aatataaaca gattcttcct gaacttcttt
     3001 gtagccctga gcaacttcat gacttgatag aggagttcca catcttgtgc aaaaaggaag
     3061 aactttgtat ccttcataaa gaagtttttt cttatataat tcctttaaac tccaccaaac
     3121 actttcaata taattatttt ctaatgtgat gtatgggttt tccaaatcaa ttaaataatt
     3181 cagttcttct gttgatttat tccagtcttc tatgtttgag aaaacactct ctctacattt
     3241 ttctatgaac ttatcaaccc catatttttc aacttctttt tttgattcaa gtttaagctc
     3301 tttttcaact tgaacttcaa caggtaatcc atgacaatcc catcctccct tacgcggaac
     3361 agaaaaacca ttcatatatc taaatttaca tattaaatcc ttgaatgttc ttgcttctag
     3421 gtgatgcaat ccaggaggag cattcgcagt agggggtcct tctagaaaac taaaaagagg
     3481 tttcttagga ttatcctgaa cagattcttt taattctttt ttgtgttttt tccagatttc
     3541 tctcgcaact ttttctacat ctttaaaatc atacattttt acttaaactt aatagagtga
     3601 ttagttttta agatttttga aaattaaaac ttaccttttt ctttgccttt tagcctttga
     3661 tttagcgttt gttttctgcg ttgaatattt atgatctttt ttatctcttt cattatagta
     3721 tgcttgatat tctctaactg ctttaacatg ttttgtttca gatgcatttg gggcaggttt
     3781 cccttggtta tatgctgata gagccttatc ccaagaacca tatctcttga aatcatctct
     3841 aaggtatttt gctgctgctt ctgcagattt tcttacatca aatcgttcat ctatcacagc
     3901 taatttccca taatcataat tatgcttttt aactaattcc tttaattcag ccccgtgggc
     3961 tttgtctgca ctagacctgt cagaagttcc gtgaactttt aatccataag cctttgctgt
     4021 tcttggttga aacataaaca atcctgctcc accatctccc atagcattta atcttaaagg
     4081 gtctccatag ctctctctca tgacaagtcc ttttaatatt cctcttggta attcatactt
     4141 atcttcaatc tcgtctaata tcttatccca tctgtaagtt ctttcaaatc ttccttgtgt
     4201 agtcatatat ggagaaacaa cttttactcc tttatttatt tcaacattct ctttggttct
     4261 tttttttagt ttatcttttc ttgcatgata agctggagct gcaataaggc caatgcttcc
     4321 tgcaattgcg gcagctttaa cccaattgga aacctttttt tctaaattac ctaaaaagaa
     4381 aattccaaga aacaaaaaaa ctaatgtaaa tattgtaatt aaagaacttt taacaccaat
     4441 accgattatt gctcctgtta tcttaacaga gaatatattt gccaaagaca aaatcataga
     4501 gaggaacaca aaaattaatc ctaatatttt cattttattc atatatctta ttaaggcaaa
     4561 tacaatttta aaagtttttg ttaaatcaat tataatagta tttaaaaaca aattttattt
     4621 tatacttaag aatggacaat tatttattac ctagattaga ttctttgtct gatgaagaga
     4681 ggcatttatt ctacaaaagg ttgacagaca tactcaatga tagatattct tgttttgttt
     4741 atggaggtat gaataatttt gaatatgcta caatgcctaa aaataagttg gaaagaatta
     4801 aaataaattt atacaatcac atgtttgaaa atataaatcc gccaatgagg gttatcactg
     4861 attttttatc tccaaacaaa gttaaagaaa aattagatat aaatttgtct aaattatcta
     4921 cactaaatat agatgataca gttttatctt ggttgtagat atataattta agaatattaa
     4981 ctaaattctt caagcatctt cttttccata aagtgaagct ctccaggaat gattatgcaa
     5041 taaggttttt gaacatttgt aaattctttt attttttcta ggtctttgta aattattttt
     5101 tgtttttttg ttccaagtgc ttgacaaata attaacttaa aaagtttaaa attatgtttt
     5161 tttgctgctt tttccagctg ttcaattgcc tttttaatat ccaatccaat atccacaaga
     5221 agaagtgtat gtgcttttat agattggttc tctttcacaa tttccataaa actatcaggt
     5281 tcataagatt ttttccattc tggtaaagaa gttattttac caaatttgta tacttgtaat
     5341 cctgtttctg caattccatc cagaacagat gcattattta taattctgta tttaatccca
     5401 gataaaatcg cttcttcaat aagggttatg tgggttgtag cagttagagg gcttccataa
     5461 acaagcaaag caacatccat tttttttgcc tcatctacaa tctcaaggct ctcaaccttt
     5521 tctctatctg cagtaacgaa cttttttcca atttcatcaa taagtgcctg atgagtgtaa
     5581 ggaaaatcta cagtataatc ctcaagataa actttcttgc atcttttgat tatttcaatt
     5641 gcttctttag acaaactttt ctcatttaat cctaatccaa ttaaatataa cataatataa
     5701 ttataaaaat aaggtttata ttatttatta ataggaattt agtttcaaag cttaatctta
     5761 aaaacccttt ctttctatta tttcaatgaa accagaggtg tattcaatcc taagtgtaat
     5821 aatagtatca ttaatctctc ttgtaggaat attttttgtt ttattgaaaa aagacaaact
     5881 caacaaaata attttatttc ttgtttcact ttcagcaggc tctcttttag ggggagcatt
     5941 tttgcattta ataccagaat catttgaaaa atcaagttca ggattaataa cttcttctct
     6001 tgtactcttg ggagtaattt tgtttttcat aattgaacat gctatacaat ggagacattg
     6061 ccatattcca acatcaaaat ctcatccaca ttccttaggt ataatgaaca ttataggaga
     6121 tggctttcat aattttctag atggtttggt tattgctgca gcatatttca taaatatacc
     6181 tcttggaatt gtaacgactc ttgcagtcat ccttcatgaa atacctcaag aaataggaga
     6241 tttcggagtt ttgatttatg caggatattc taaaaagaaa gcccttttct ttaatttcct
     6301 ttcagcttta acagcagttc ttggtgcaat tgtaggaatt ttatttgcag gtagcagtga
     6361 tatgtttgca ttaatcgcaa tacctattgc agcaggaggt ttcctatata ttgctggtag
     6421 tgatttaatt ccagaactgc ataaaaatca agaaaaacat ttttcaataa ataagattat
     6481 agggattata ttaggaattc tactaatgta tggattaact tttttgaaag tggcttaatt
     6541 tataacaata attttttatc gtaataatat ttataaattt cttggagtat tcttttttat
     6601 ggttgaaata gggaaagttc tggagttatt caataaaaca aatccaagta atgttgaaaa
     6661 aatatggaag ggttttatga taaaaaagaa ggatttttat ttttattctc ataatctttt
     6721 tagtgagatg tatgaattaa ctatctttga acaaggaaac atagttcctc tccaaaaatt
     6781 tgtttataat gaaagaaata aagaaggaga taaaaaagta gaagagtgtt ataatcttct
     6841 tcatagaaaa tactttgaat ggaagaaaca tgaagaggaa gttatgattt ttgaagccca
     6901 agaacaaatt gaaaagaata aacgagaaga agaaatgaaa gatgtattaa atgaagcaga
     6961 aagaatccag tcatttaaaa agcttgaaaa aattctgtct taaatatttt attttttgtt
     7021 agctaagaaa actccaagaa gtcctaagaa aaataatgct gcaccgtgta taacagaacc
     7081 actaacaaca tctgcaatcg cattacctgt taatgcagga tatcctattg caactcccaa
     7141 tatcattgaa cctaaggaaa taattgttat tgctttttca agtccagaag atttattttt
     7201 tttgagatgt ttcttacaat atttcaaaat tactttattg tcttcaaaat agttttctga
     7261 tttatctgat tttgcaatca atctagaacc tcttcttgct aaagcttcct gatatttttc
     7321 aactttgtct ttaggcatta tttgttcttg gtccaatctt ttttgaaggt ttaaaaatgt
     7381 aaaatacgct tctctcaaat ctcctgaacg tattttttcg tccattgaag ctaatccaaa
     7441 agtatatttc tcttctctta tttccacact atctcctcta ctagaagaag gagtatctat
     7501 ctttctagct attccataag cactaagagg attatattta gcaagtgaag ccatgtgttt
     7561 tcttttacta aattcttcat tccctttcct agatttatag taaggttctt tcaccatttt
     7621 ttacaaatta ttcaagattt ttgaacttat aaatatttct agtacaaggt ttatttattt
     7681 gccatttaga actttcaacc cttcaatgca atcttcaaca gaataaggac tctcgcatac
     7741 aattgttata tctttatctt taggcaagtt ttcaaacaat tctttccatt gttttttctc
     7801 gactctaata tgatttcttt ctcctttatc accatactct attcctgaaa aatggcaatg
     7861 ccattttttc tgtggaaata atctttttat tttttcataa tcaacttttt tttctcttgc
     7921 taaaatatga gcaaaatcaa tacaaaaact acatccagtt tctttaacta atcttgaaat
     7981 ttcctcagga ctcccaaaaa catttacctt acccatagtc tcaggacaga gttcaacatc
     8041 ccatttcttt ttttcaattg tttccatgat ttcaagaacc ctttttttaa tattttggaa
     8101 tgtttcttct tcaactcttt ttttgtcttc taatgtttta gctttttgaa attcttttcc
     8161 ataaaaccct ggatgaaaga caatactctt agcacctaat tcatgtccta tttcacaaca
     8221 ttttaagatt ctttgcatac ttgcatgaac tttctctttt tcttcagaat ttaaattaat
     8281 aaaatattga gcatgaacac ttaaatcaat tttatttttc tttgcaaatt cttttatttc
     8341 tttagcttct tctttatcta tataaacttg ctgtacaaaa agaacttcac aattattgaa
     8401 tccgtttttt gagaattctt gaagggtttt ttcactttct ctgacacttc ctaaaccagt
     8461 tggacctatt tttatacttc ccatataaac aaaaacaaaa aagagttttt aagattaatc
     8521 taagtacatg taggacaata cgctgtataa atttcatatg ctgaattagc atatgcaggt
     8581 acactatgag ccttggtttt agtagttgat cctatccaat ctggaacaag ggaatattct
     8641 ggatttttat ttgttttttt aagatattct tcttttatgt aattgtaaat gtctgattct
     8701 tcaaatgatt tactataaaa tgtaccgctg gatattggtt taagttttct ttctctagca
     8761 aaattaatta tggccttttc tgtttctcct ccccatagtc catctgcctt tgcattatag
     8821 taccccaatt cttttaattg tgcttgaact gcagcatttc gtggagtata ttttccaaag
     8881 ttataagctg cagcaataga tttaacttgg ttttctgtca ttcctccagg ggttggcgaa
     8941 tatatattaa ctaattctgc taaatatctt tgtccatatt ttatagaaac ttctttatct
     9001 cgatacatct ctattgtcga aggtgttttt tcattgaatt ctttagcata tttttctata
     9061 tctgcagtat ttatttgcat gtatcccaat gttgggtttt ttgatactgc tttatctaat
     9121 ctagttctag ccaatgtaga ctctgcagca taccatttag aatctcccca attagactct
     9181 ttagttataa ttgtagcagt taatgcatta aattcattaa atttagaatc aggtatatta
     9241 tattcttttt gcctctcagt acttgatttt agtagtgctt ggttccaatc agtatatttt
     9301 ttaacattat tagccctaag caattcttct aaattaaatc cagtgcagct aattgcatta
     9361 caatctatgt ggttttggta agtagttaca atatctggag ttacaaaaag ttcattaagg
     9421 tactggcttg atttaggtct agttacacca tataaagagt aacttggttg atctaaaagc
     9481 acatctaatt ctctaacatt aaattgaccg cctataaaat ctgaaactac aggagtagat
     9541 ataagtattt cttttccaga atcaaaagat aactctttcc catcttcagt atagaatttt
     9601 ttgtttttta atgtgagagg tttaacataa cctgtttcct catcaatgta attataggtg
     9661 ttaacataca ttgagtcatc atttttgact cttaatttcc cctttaattc agtaaataca
     9721 ctttcagatt ctccttttat tattaaactt tgttcatttg atccaacaat tactccaata
     9781 tgggtatttt tttgactatt ttttccttct attgtagctt gtgttaaata gtgtgaagaa
     9841 ggataataaa acgagacaat atctcctttt tgaggagtga cttgttgagg aatataatct
     9901 ttacttaata tactctgcat tttattaata gaatctgaat ttgttgtaga gattttatta
     9961 aaaataactt catcgcttaa tctcagcttt tttagcctgt tatactcttt tttcaattca
    10021 gtttcaatct ctttaagttg tgttctttct gcagttgtta gttcatgttc tttactatat
    10081 actattcctc ctccctgttt taacatatta tactgaatct cccaagcatc tccatggact
    10141 ccattttcac gtaaatattt ctgagcatca ccttgattag ttaattttat tccaccacca
    10201 tattcttctt ctaccaataa gcaacctacc tgtgctcctc cagaacatac gactttgttt
    10261 acatccaatc caagaatttt tgcaccgcgt gcaagttcaa cacttcctat tttattatta
    10321 ggactaatca aatctaattt caactcttta ccttcattat caatatattt aattgtaatc
    10381 ggagtagata ctgaagtaga ttcaagagag gttcttaaat tttctttaaa ctctcctccc
    10441 tcaaataaat atttttttcc agctatttca agagatcctc tacctttcat tgtaatatct
    10501 aatccatttt taccattagt tattgttgaa gaccctccca tcatatttag attaatggtt
    10561 tctttcgata attttgttgc tttttcaaca gaaagtttcc caaattgccc atctggagta
    10621 agttgattat ctttttgcca ttctttcaat gcattttctg ttctaaatcc aaaatatcca
    10681 tcaactgaat ttacttcttt accataagca tttgtaaatg tttttgataa atacccttga
    10741 gaaattaaca tactttgaat actttttaca tcctcacctt tatcccctga tttaaacgat
    10801 tttccattgg aagatttaga taaatcgaaa ttatacacat aattagcttt ttctaaagag
    10861 ttttttccaa atttaccatc actagccaac ccattctctg cttgccactt gattactgct
    10921 tgttctgtta ttttatca
//
LOCUS       DAOPCH010000025        10721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_5996_length_10721_cov_4.818207, whole genome shotgun sequence.
ACCESSION   DAOPCH010000025 DAOPCH010000000
VERSION     DAOPCH010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5996_length_10721_cov_4.818207"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="PLU55_02700"
     CDS             complement(<1..1035)
                     /locus_tag="PLU55_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPJ86997.1"
                     /translation="MEILDRITEKLHGKVKEANFEGANIVLYTDNEKFFKEGEGQIKK
                     IVDEIKKRIELRADQKILSPKEATEKKIREIVPEEAEITEILFDAPRSIVVIEAKKPG
                     LVIGKGGSILEDIRKETLWVPQVQRSPAIRSKITENIREVLYDNNNYRRKFLNDIGEK
                     IYKGWNPDKIEEWVRVSFLGSGRQVGRSCLLLQTPNSNILLDCGIDVASSGNDKFPYF
                     NIPEFDISQLDAIILSHAHLDHSGLLPYLYKMGYRGPVYMTAPTRDIAALLALDFIGV
                     AYKQAAAPLFSSADIKEMVKHSICLNYNEVTDVTPDIRITLYNAGHVLGSAQVHINIG
                     NGLHNFVYTGD"
     gene            complement(1055..1720)
                     /locus_tag="PLU55_02705"
     CDS             complement(1055..1720)
                     /locus_tag="PLU55_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HPJ86998.1"
                     /translation="MDEELKKSMLKTGTTILGIVCKDGIVMASDRQVSAGNLVVSKNY
                     AKTIKVNDYLLVSFTGMVSDAQRVVKILPAELKLKELRAKSRPTVEQAANLLSSILYS
                     GIRQPSMIPMQVGNLIGGFNEDGTIELYTVEPAGSVVKVEDYDANFGSGMPYVLGLLE
                     RQYKKDLTLKEGVELAKEALKSSTQRDMGSGYGIDVFTITKEGIKKVVEQQILPEYID
                     EKK"
     gene            complement(1822..2097)
                     /locus_tag="PLU55_02710"
     CDS             complement(1822..2097)
                     /locus_tag="PLU55_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010870686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin RelE"
                     /protein_id="HPJ86999.1"
                     /translation="MFDFDISDELKLIIMKLVKKDKKKVEIINKKIKEIINCDNVSIN
                     HYKNLRYDLKEFKRVHIDKNFVLIFKVDLTNNFILFVDFDHHDRVYK"
     gene            complement(2098..2361)
                     /locus_tag="PLU55_02715"
     CDS             complement(2098..2361)
                     /locus_tag="PLU55_02715"
                     /inference="COORDINATES: protein motif:HMM:NF022340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2683 family protein"
                     /protein_id="HPJ87000.1"
                     /translation="MENKTISARVELNDYTNRVLGVIKMKFGLKDKSEALNKFIELYG
                     DDVIEREAKDEYIKNVIRISETHLKKYGKRKMTLQELNKLCEE"
     gene            complement(2474..4525)
                     /locus_tag="PLU55_02720"
     CDS             complement(2474..4525)
                     /locus_tag="PLU55_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HPJ87001.1"
                     /translation="MKIKRIILKNIRSYEYQEITFPEGSVLLSGDIGSGKTSILLAIE
                     FALFGLQPGQKGSSLLRNGQVNGGVEIEFEVDNKTIIIERTLKKTKSISQDYCSISID
                     GDKKEISVTELKSKVLEILNYPKEFSKKQNILYKFTVYTPQEEMKQIILEDSETRINT
                     LRHVFGIDKYKKILENASVLIAKVREEKRIKDAITSNLEHDKLHIETKEKELSEKSFS
                     ILSFEDDLKKKSEKRKIIQEEKEEIFRKIDEKRKLQQEIEKTIFLRSTKEDTISSNSR
                     MMTQLKSQIQELKDLDYDEKEIEKLEKDISELKLKKQELNEQNIHITSLIHSLNSKNS
                     DNEKVKEKLKHLEVCPTCLQEVDPVYKSNVINQMDSDTSANNNLIKENTEKKEAIRDQ
                     MLKLDYDSSIKEKRIHELRILKVRLRELDDKQRHLTTLEKTNLALTKDLEMLDSQINL
                     LKSSVFELNKYDNINDAKQRELNEALREEKLAEIKVAELKREIEVFSRQINEMKERVK
                     KTEEIKQQAHYLSDLEEWLSKKFIPIILVVEKNVFTKLKIGFSKLFSKWFSMLVSDSF
                     NVRLSEDFTPIIEQQDYEIDYAYLSGGERTAIALAYRLALNQVINSFLSKIKTRDLVI
                     LDEPTDGFSEQQLDKMREVLDELNVSQLIIVSHEQKIESFVDNVIKFKKENGRSIIET
                     K"
     gene            complement(4531..5703)
                     /locus_tag="PLU55_02725"
     CDS             complement(4531..5703)
                     /locus_tag="PLU55_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HPJ87002.1"
                     /translation="MVRFAHIADVHLGGWKQQPMQNLNFESFKEAVNICITSKLDFVL
                     FSGDLFDSAYPPIEILKETFAQFKRLKDAGIPCFTIAGSHDYSVSGKTFLDVLEKAGF
                     CKNVYNAEEKENSITLNPVIYGNVAIYGYPGRKSGLEIADLKKVKFNDAPGLYKIFML
                     HTTIDRVVGTLPIESINPDRLPHADYYALGHIHVFYSDRGFVYPGPLFPNNFKELEDL
                     KQGSFVIVDTESNSRLQRIKLPTKQIEHMVIEIEDALTGTEKIIYELNKRNLKDKIIL
                     LRVYGELKRGKSSNIKFSKIEEFVKGKEAYFLLRNTHDLISEEQELDLKLPEKDSENI
                     EEETIKVYSEENPSSFNKIIPELMNALSIEKQEGETTETFNNRILQGTKKFFKFPN"
     gene            5855..7336
                     /locus_tag="PLU55_02730"
     CDS             5855..7336
                     /locus_tag="PLU55_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPJ87003.1"
                     /translation="MRYNILIGGEAGQGPNILTQIIGDAIIKSGNYAFYSRDYQSLIR
                     GGHNFNVITFSDEPVSSNDSKIDILVCLDSKTEELHKKNLKTGGILLKDSKENMYYAG
                     RIFKLLCMDFKLLEEGLRKLEKNFNENLANAKRGYDEETRNVCKIRTVKKESYFINGN
                     IGICNGALNSGIDIYYAYPMTPATPILSELAPVDGVKVIELENEIAVVNAGLGSAALG
                     KNVMVGTSGGGFDLMTEGLSMAGVAEIPIVVYLAQRPGPGTGVATYTSQGDLNMALNF
                     GHGEFPRTVAAPGDPKEAQEITNEAFYLSQKFKIPSIILGDKHLAESYYTLEEESSIK
                     KVENTTQLIRYNSYEKDDIGSATENAQIIKANVDRRLKKTQEIKREVEKFPRFSVYGE
                     TDSMNVVVSWGSTKGAILDAIKDLDVEFLHIKYLEPFSDGIKENLLRKNIILVENSST
                     GQLAGVISKHTGMFIQDKNKILRYDGRPFLADELRKEIEKRLI"
     gene            7333..7935
                     /locus_tag="PLU55_02735"
     CDS             7333..7935
                     /locus_tag="PLU55_02735"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPJ87004.1"
                     /translation="MKIETQRLTLREPRIGDWKDLVDGLNNLNISKNTLTIPYPYTKK
                     DALEYLNRCSKKKKDKEAYKFFIELRKEKKVIGAIDLNDYKKDQGTAETGSWINERYQ
                     KKGYITEAKIAVNDFVFHKLKLRKLKSKVFKENKASQKTQERMGYQKEGVLRKESICK
                     PTKKIHDDIVYGLLKEEWEKARPKVIKHLNKKIKKLENRK"
     gene            7932..8378
                     /locus_tag="PLU55_02740"
     CDS             7932..8378
                     /locus_tag="PLU55_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87005.1"
                     /translation="MKKNKTRSKKTKETAKKQKVKNQENKKLNTKTEQQLIWISYTAI
                     LMVIGLIFFKYLPMYLSEGNILYDASYHVLFTILLLYILWFFIDQKKSWRIPYFIFSG
                     VLIIIVSLQRIIAQEHNEVGIILALLIGAVSIIIPRWKEFMGGVKF"
     gene            8385..9179
                     /locus_tag="PLU55_02745"
     CDS             8385..9179
                     /locus_tag="PLU55_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPJ87006.1"
                     /translation="MSANLNTNTKPTWCPGCWNFQILAGVKNFLSKKIKNDGDKKNYA
                     IVTGIGCHAKIFDYINLNGINSLHGRALPTALGVKVASPNLNVIAFSGDGDAYAEGME
                     HLIHAARYNSDIKYLVHNNQVFALTVGQPTPVTEVGFKDKTNPRGVRVPPINPIKLML
                     SAGATFVARVYADAKQIEEVLEEAFKHKGFAFIEVIQPCLIFHPNTNYKKYTYMLKEK
                     KHNEKSFEAAMKRAQEFDYNGINRKTKIPLGIFYKEKRDVFEKNIR"
     gene            complement(9172..9723)
                     /locus_tag="PLU55_02750"
     CDS             complement(9172..9723)
                     /locus_tag="PLU55_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPJ87007.1"
                     /translation="MASIYQPAEDSYLMSRILKEQLPELLNKNPNLRFLEIGVGSGIH
                     LQTASRLGVKKENIFSSDINKNSVNHCNLLGFNCIYSDLFENIEGKFDLIIFNPPYLP
                     QDSNEPEDSSLATTGGVKWNEIILRFLNDAKFHLEKNGKIFLITSSLSEDINFQGLGY
                     VEKELSCEKLFFERLCIWELKLI"
     gene            complement(9754..10455)
                     /locus_tag="PLU55_02755"
     CDS             complement(9754..10455)
                     /locus_tag="PLU55_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87008.1"
                     /translation="MAKVNEEIVGGLISALSRKETLEKAMMTFYNAGYEKEEIEDSAK
                     DVYNKLGSKAMGINGSLQEALDDIANKAGVTKKEKPKEEESSEEVSPDPNLNIKHIPE
                     KKPETKPELIPDLSVNEDISSNKQVSIYGSDDSPSNYQNPDELTKKIEEAIKGLRPVN
                     IPSKIEIVNRSEFSKSPTVVQHVSGYTEPQIKPASKAVTYMLIFILIILLGALVAVFL
                     FKDELIRLFNELGLG"
     gene            complement(10458..>10721)
                     /locus_tag="PLU55_02760"
     CDS             complement(10458..>10721)
                     /locus_tag="PLU55_02760"
                     /inference="COORDINATES: protein motif:HMM:NF019159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HPJ87009.1"
                     /translation="SVNINAREKMDNKLRKQGVRLQTDVHVSGHGGREDLRDLLGMLK
                     PEHLIPAHGTLKQEAPLIDLAKEMGYKFQENSHLSSNGKVIKI"
BASE COUNT         3577 a         1619 c         1511 g         4014 t
ORIGIN      
        1 atctcctgta tatacaaaat tatgcaaacc atttcctata ttgatatgaa cttgtgcaga
       61 acccaataca tgtcctgcat tgtagagagt tattcttata tctggtgtaa catcagttac
      121 ttcattgtaa ttcaaacaaa tactgtgttt taccatctct tttatatctg ctgaactaaa
      181 taaaggagca gcagcttgtt tgtaagcaac acctataaaa tccaaagcaa gaagagctgc
      241 aatgtctctt gtaggagcag tcatatatac aggtccacga tatcccatct tgtaaagata
      301 aggaagtaat cctgaatggt ctaagtgtgc atgactcaat ataattgcat ctaattgaga
      361 gatatcaaat tctggaatat taaaataagg gaatttatca tttcctgaag aagcaacatc
      421 aattccacaa tctaaaagaa tatttgaatt aggggtctgt aaaagcaaac agcttcttcc
      481 aacttgtctt ccacttccta agaaactcac cctcacccac tcttctattt tatctggatt
      541 ccatcctttg taaatttttt cacctatatc attaaggaat tttcttctgt aattattgtt
      601 atcataaaga acttctctga tattttctgt tatcttgctt cttattgcag gagaacgctg
      661 aacctgtgga acccagagag tttcttttct aatatcctca agtatagaac ctccttttcc
      721 aataactaat cctggttttt tagcttctat aacaactatg cttcttggag catcaaataa
      781 aatttctgtt atttctgctt cttctggaac tatttctctt atttttttct ctgttgcttc
      841 ttttggagat aaaattttct gatctgcacg aagttctatt ctttttttta tttcatcaac
      901 tatctttttt atctgacctt ctccttcttt gaaaaatttc tcattatcag tatatagaac
      961 tatatttgct ccttcaaaat tagcttcttt aacttttcca tgtaattttt ctgtaattct
     1021 atctaaaatt tccattttaa aaatatatgt aaatttattt cttctcgtct atatattccg
     1081 gaagaatctg ttgttcaacg acttttttaa ttccctcttt tgttattgta aacacatcaa
     1141 ttccatatcc tgaacccatg tctctttgag ttgaagattt taaggcctct tttgcaagtt
     1201 caactccttc tttaagagtt aagtcctttt tgtattgtct ttctaataat cctaaaacat
     1261 aaggcatacc tgaaccaaag tttgcatcat aatcttctac tttaacaaca cttcctgctg
     1321 gttcaacagt gtagagttct attgttccat cttcattgaa acctcctatt aaatttccaa
     1381 cttgcattgg aatcattgaa ggttgtctta ttccagaata tagtatactt gaaagtaagt
     1441 ttgctgcctg ttcaacagta ggtcttgatt tagccctgag ttccttaagt tttaattctg
     1501 ctggtaaaat ttttacaact ctctgagcat cagaaaccat gcctgtgaaa gaaacaagaa
     1561 gataatcatt tacctttatt gtctttgcat aatttttgct tacaaccagg tttcctgcag
     1621 aaacttgtct gtcagaagcc ataacaatac cgtctttaca aacaattcct aatattgtcg
     1681 tacctgtctt taacattgat ttctttaatt cttcatccat tttagatttt ccgataaaat
     1741 caattcagaa tttatattat atgtaaaatt gatgatttta taaatttttt ggttgtggaa
     1801 aaattgttaa tatctgagga tttatttata caccctatcg tgatggtcaa agtccacaaa
     1861 taaaataaag ttattagtta aatctacctt gaatattaaa acaaaattct tgtcaatatg
     1921 cactctttta aattccttta aatcatacct taaattcttg tagtggttta tagaaacatt
     1981 atcacaattt attatttcct taatcttttt gtttataatt tctacttttt tcttgtcttt
     2041 ttttactaat ttcataataa taagtttcaa ttcatcagaa atatcaaaat caaacattta
     2101 ctcctcgcag agtttgttta attcttgtag agtcattttt ctttttccat acttcttaag
     2161 atgagtctca gatattctta ttacattttt aatatattca tcttttgcct ctctttctat
     2221 tacatcgtct ccataaagtt caataaattt attcaatgcc tcagatttat cttttaatcc
     2281 aaatttcatt tttataactc ctaaaactct gttagtgtaa tcatttaatt ctactctggc
     2341 agaaatcgtc ttgttttcca tgctctctcc ttgcatttat ttaatgtgta tatactgtgt
     2401 atttaatatg catttcatat gcatataaag tttataaatt tttggttgtg gaaaaagcaa
     2461 gatattgcta attttattta gtttcaataa tgcttctccc gttttctttc ttgaatttta
     2521 ttacattatc tacaaagctt tcaatcttct gttcatgact tacaattatt aactgggaaa
     2581 cattcaattc atctaaaacc tctctcatct tatctaattg ctgttctgaa aaaccatctg
     2641 ttggttcgtc aagtataact aaatctcttg tttttatttt actcaaaaaa gaattaatta
     2701 cttgatttag tgcaagtcta tatgcaaggg caatagctgt cctctctcct cctgaaagat
     2761 aagcataatc tatttcataa tcctgttgtt ctattattgg tgtaaaatct tcacttaatc
     2821 ttacattaaa actatctgac actaacatag agaaccattt agaaaaaagt ttagagaatc
     2881 ctattttcag tttagtgaaa acattttttt caacaacaag aattattggt ataaatttct
     2941 ttgaaagcca ttcttctaaa tcagaaagat aatgagcttg ttgttttatt tcttctgtct
     3001 tttttacacg ctctttcatc tcatttattt gtcttgaaaa tacttctatt tctcttttca
     3061 gctctgcaac ttttatttct gctagttttt cttctctcaa agcttcatta agctctcttt
     3121 gctttgcatc attaatatta tcatatttgt taagttcaaa aacacttgat ttaaggagat
     3181 ttatttggct gtcaagcatc tctaaatctt tagttaaagc aagatttgtt ttttccagtg
     3241 ttgttagatg tctttgttta tcatcaagtt cacgtaatct tactttcaat attcttaatt
     3301 cgtgaattct tttttctttt attgaagagt cataatcaag tttaagcatt tggtccctta
     3361 tggcttcttt tttctctgtg ttttctttta tcaaattatt atttgcagaa gtgtctgaat
     3421 ccatttgatt aataacattt gatttgtata ctgggtcaac ttcctgaaga catgttggac
     3481 agacttcaag atgtttaagt ttttctttta ctttttcatt gtcagaattt ttagaattaa
     3541 gagaatggat taaacttgta atgtgtatgt tttgttcatt taactcttgt ttctttaatt
     3601 ttaattcaga aatatctttt tccaattttt ctatttcttt ttcatcataa tctaaatctt
     3661 ttagttcctg aatctgagat ttaagctgag tcatcatgcg tgaattgctt gaaatagtgt
     3721 cttctttagt tgatctaaga aatattgttt tttcgatttc ctgctgaagc tttctctttt
     3781 catctatttt tctaaatatc tcttcttttt cttcctgaat aatttttctt ttttcagatt
     3841 ttttctttaa atcatcttca aatgaaagta tgctaaatga tttttctgaa agttcttttt
     3901 ctttggtttc tatgtgtaat ttatcatgtt caagatttga agttatagca tcctttattc
     3961 ttttttcttc tcttacttta gcaattaaca cagaagcgtt ttcaagtatt tttttgtatt
     4021 tatctattcc aaaaacatgt cttaaagtat ttattcttgt ctctgaatcc tcaagaatga
     4081 tttgcttcat ttcttcttgt ggagtataaa ctgtgaactt gtaaagtata ttctgtttct
     4141 ttgaaaattc tttcggatag tttaatattt caaggacttt actttttagt tctgtaactg
     4201 aaatttcttt tttatctcca tctattgaaa ttgaacagta atcttgagaa atagattttg
     4261 ttttttttaa ggttctctca ataattattg ttttgttgtc aacttcaaat tctatttcaa
     4321 ctcctccatt aacttgccca ttgcgtaaga gagaagaacc tttttgtcca ggttgtaaac
     4381 caaataatgc aaattctatt gccaaaagta ttgaagtttt acctgagcca atatctcctg
     4441 aaagaaggac tgaaccttct ggaaaagtta tttcctgata ctcataactc cttatgttct
     4501 ttaatattat tcgttttatt ttcattttaa ttaattagga aatttaaaga actttttagt
     4561 tccttgaagt atcctgttat tgaaagtttc tgttgtttct ccttcttgtt tttctattga
     4621 aagtgcattc attaattctg gaattatttt attgaaagaa gaagggtttt cttcagaata
     4681 aacctttatt gtttcttctt ctatattctc tgaatctttt tcaggtagtt taaggtctag
     4741 ttcctgttct tcagaaatta aatcatgagt atttcttaat aaaaaataag cttctttgcc
     4801 tttaacaaat tcttcaatct tagaaaattt tatattagaa gattttcctc tttttaattc
     4861 accatagacc ctaagaagaa ttattttatc tttaagattt cttttattta attcataaat
     4921 tattttttct gttcctgtta atgcatcttc tatttcaata accatgtgtt ctatttgctt
     4981 tgttggcaat tttattcttt gtaatcttga attagattca gtatcaacaa ttacaaaaga
     5041 accttgcttt aaatcttcaa gttctttgaa attgtttggg aatagtggac ctggataaac
     5101 aaatcctcta tcgctgtaaa aaacatgaat atgcccaaga gcataataat ctgcatgagg
     5161 aagtctatcc ggattaattg attctattgg gagtgttcct acaactctgt ctattgttgt
     5221 gtgaagcata aatattttgt ataaaccagg agcatcattg aatttaactt ttttcaaatc
     5281 tgctatttct aaaccagatt ttctacctgg atatccatat attgcaacat ttccgtaaat
     5341 aacaggattt agagttattg aattttcttt ttcttctgca ttatatacat ttttacaaaa
     5401 tcctgctttc tccagaacat ctagaaaagt tttaccagat acagaataat catgagaacc
     5461 tgctattgtg aaacaaggta ttcctgcatc tttaagtctt ttaaattgtg caaaagtttc
     5521 tttcagtatc tctattggag ggtatgctga atcaaataaa tctcctgaaa acagcacaaa
     5581 atctaattta gaagttatgc atatattcac agcctctttg aaggactcaa agtttaaatt
     5641 ctgcattggt tgttgtttcc atcctccaag atgtacatct gctatgtgtg caaaccttac
     5701 cattttagca gactcctcct aaagtcgtcg tgagcaaacc ttaccatttt agtattaatt
     5761 taatgaatat ttttaactat ttttatacaa aaaagtttat aacagctcaa aactattact
     5821 atatataaaa ttaattgcga taaagaggta caaagtgaga tataatatac ttataggggg
     5881 tgaagcagga caaggtccaa atattttaac tcaaattata ggtgatgcta ttataaaaag
     5941 tggaaattat gctttttatt caagagatta tcaatcgctt ataagaggag gacacaactt
     6001 taatgtaatt actttttcag atgaacctgt atctagtaat gattcaaaga tagatatttt
     6061 agtttgttta gatagcaaaa cagaagaact ccataaaaaa aacttgaaaa caggaggaat
     6121 attgcttaaa gattctaagg aaaatatgta ttatgctggg agaattttca aattattatg
     6181 tatggatttt aaacttcttg aagaaggatt gagaaagcta gagaaaaatt tcaatgaaaa
     6241 tcttgcaaat gcaaaaagag gatatgatga agaaacaaga aatgtatgca aaataagaac
     6301 agtaaaaaaa gagagttatt tcataaatgg aaatattgga atatgtaatg gggctttaaa
     6361 ttcagggata gatatttatt atgcatatcc gatgactcct gcaacaccta tacttagtga
     6421 gttagctcca gttgatggtg taaaagtaat agaattagaa aatgaaattg ctgttgttaa
     6481 tgcaggatta gggagtgcag cattaggaaa aaatgttatg gttggaactt ctggaggagg
     6541 atttgattta atgactgagg gattaagtat ggcaggtgtt gcagaaatac ctattgttgt
     6601 ttatttagca caaagacctg gtcctggaac aggtgttgcg acttacactt ctcaaggaga
     6661 tttgaatatg gcattgaatt ttggacatgg agaatttcca agaacagttg ctgcaccagg
     6721 agacccaaaa gaagcacagg aaataacaaa tgaagcattt tatttatctc aaaaattcaa
     6781 aataccttct ataattcttg gagataaaca tcttgctgaa agttattaca cacttgaaga
     6841 agaatcttct ataaaaaaag tagaaaatac aacacaactt ataagatata atagttatga
     6901 gaaagacgat attggaagtg ctacagaaaa tgctcagata ataaaagcaa atgtagatag
     6961 gagattaaaa aaaactcagg aaataaaaag agaagttgaa aaattcccaa gattttctgt
     7021 ttatggtgaa acagattcta tgaatgtagt tgtaagctgg gggtcaacta aaggagctat
     7081 acttgatgca ataaaggatt tagatgttga atttttacat ataaaatatc ttgaaccttt
     7141 ttcagacgga ataaaagaaa atcttctgag gaaaaatata attcttgtag agaattcttc
     7201 aacaggacaa ttagcaggag ttatctcgaa acacactggt atgtttatac aagataaaaa
     7261 taaaatacta agatatgatg gacgtccttt tcttgcagat gaattaagaa aagaaataga
     7321 aaagaggtta atatgaaaat agaaacacaa agattgactt tgagagaacc aagaattgga
     7381 gattggaaag atttagtaga tgggttaaat aatctaaata tctcaaaaaa tactcttacc
     7441 ataccctatc catacactaa aaaagatgct ttagaatatt tgaatagatg ttctaaaaaa
     7501 aagaaagata aagaagctta taaatttttt attgaattaa gaaaagagaa aaaagttatt
     7561 ggtgcaattg atttaaatga ttataaaaaa gatcagggaa ctgcagaaac aggttcatgg
     7621 ataaatgaaa ggtatcaaaa aaaaggatat ataactgagg ctaaaattgc agtaaatgat
     7681 tttgtatttc ataaattaaa attaaggaaa ctaaaatcaa aagtttttaa agaaaataaa
     7741 gcctcacaaa aaacacaaga aagaatggga taccaaaaag aaggtgtttt aagaaaagag
     7801 tcaatatgta aaccaactaa aaaaattcat gatgatattg tttatggttt attaaaagaa
     7861 gagtgggaaa aagctcgtcc taaagttatt aaacacttaa ataaaaagat aaaaaaatta
     7921 gaaaacagaa aatgaaaaaa aataaaacaa gaagcaaaaa aacaaaagaa acagcaaaaa
     7981 aacaaaaagt aaaaaatcaa gaaaataaaa aattaaatac aaaaactgaa cagcaattaa
     8041 tttggatttc ttatactgca atcttaatgg taattgggct aatatttttt aagtatttac
     8101 caatgtattt atctgaggga aacatattat atgatgcatc ctaccatgtt ttatttacaa
     8161 ttcttttatt gtatatactc tggtttttta ttgaccagaa aaaatcttgg agaattcctt
     8221 attttatatt ttcaggagta ttaataataa ttgtaagttt acaaagaata attgctcaag
     8281 aacacaatga agttggaatt atacttgctt tgcttatagg agcagtttca attataattc
     8341 caagatggaa agaatttatg ggaggtgtta agttttaaaa taaaatgtca gcaaatttaa
     8401 acacaaatac aaaaccaact tggtgtccag gatgctggaa ctttcagata cttgcaggtg
     8461 ttaaaaattt cttaagtaaa aaaataaaaa atgatggaga caagaaaaac tatgcaatag
     8521 ttacaggaat aggatgtcat gctaaaatat ttgattatat taatttaaat ggaataaatt
     8581 ctcttcatgg aagagctctt ccaacagcat taggagtaaa agttgcaagt ccaaatctga
     8641 atgtaattgc attctctgga gatggagatg cttatgcgga aggtatggaa catttaattc
     8701 atgctgcaag atacaatagc gatataaaat atttggttca taataatcaa gtatttgctt
     8761 taacagtagg gcaaccaacc cctgtaacag aagtaggatt caaagataaa acaaatcctc
     8821 gtggagttag agttcctcca attaatccaa taaaattaat gctttctgct ggagctactt
     8881 ttgttgcaag agtttatgca gatgcaaaac aaatagaaga agttcttgaa gaagcattta
     8941 agcacaaggg atttgcattt attgaagtta tacagccatg cttaattttc catcctaata
     9001 caaattataa aaaatacaca tatatgttga aagaaaaaaa gcataatgag aaaagctttg
     9061 aagctgctat gaaaagagcg caggaatttg attataatgg tataaacaga aaaacaaaga
     9121 ttcctttagg aatattctat aaagaaaaaa gagatgtttt tgagaaaaat atcagataag
     9181 tttcaattcc cagatacata atctttcaaa aaataatttt tcacagctta attctttttc
     9241 cacataccct aaaccttgga aattaatatc ttcagataaa ctgcttgtga ttaaaaatat
     9301 ttttccgttt ttttcaaggt gaaattttgc atcatttaaa aatcttagaa taatctcatt
     9361 ccatttaact cctcctgttg ttgcaagaga cgaatcttct ggttcatttg aatcttgagg
     9421 aagatatggg ggattaaaaa ttatcaaatc aaattttcct tcaatattct caaataaatc
     9481 ggaatatata caattaaatc caagaagatt acaatgatta acagaatttt tgtttatatc
     9541 tgaagagaaa atattttctt ttttaacccc taatctactt gctgtttgaa gatgtattcc
     9601 tgagccaact cctatttcta ggaatcttaa attaggattt ttatttaata attctggtaa
     9661 ttgttctttt aatattcttg acattagata agaatcttca gctggttggt atattgaagc
     9721 catttcttat taaaaaagaa gcatattatt aatttatcct aaacctaatt cattaaacaa
     9781 tcgtattaat tcatctttaa acaagaaaac tgcaactaat gctcctaaaa gaattataag
     9841 gataaatatt aacatataag ttacagcctt gcttgctggt tttatttgag gttctgtata
     9901 acctgaaaca tgctgaacaa ctgttggaga tttagaaaat tcactcctat taactatttc
     9961 tatttttgat ggaatattta ctggtcttaa tccctttatt gcttcttcta tttttttagt
    10021 taattcatct ggattttgat aattagaagg agaatcatct gaaccataaa ttgaaacttg
    10081 tttattactt gaaatatctt cgtttacaga taaatctgga attaattctg gttttgtttc
    10141 aggtttcttt tctggtatat gtttaatgtt taaattaggg tctggagaaa cttcttctga
    10201 ggattcttct tccttaggtt tttctttttt agtaacacct gctttatttg caatatcatc
    10261 cagagcttcc tgcaatgagc cattaatacc cattgctttt gagcctaact tgttataaac
    10321 atcttttgca gaatcttcaa tttcttcttt ttcatatcct gcattataaa aagtcatcat
    10381 tgctttttca agtgtttctt ttcttgatag agcagatatt aatcctccaa ctatttcttc
    10441 atttaccttt gccatcttta aatctttatt accttaccat ttgaagataa gtgcgagttt
    10501 tcttgaaatt tatatcccat ctcttttgct aaatcaatta atggggcttc ctgttttaat
    10561 gtaccatgag ctggaattaa atgttctggt tttaacattc ccaacaaatc tcttaaatct
    10621 tctctacctc cgtgtcctga aacatgaaca tctgtttgca atcttactcc ctgttttctc
    10681 aacttgttat ccatcttttc acgcgcattg atattaacag a
//
LOCUS       DAOPCH010000026        10241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_6347_length_10241_cov_4.089240, whole genome shotgun sequence.
ACCESSION   DAOPCH010000026 DAOPCH010000000
VERSION     DAOPCH010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10241
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6347_length_10241_cov_4.089240"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PLU55_02765"
     CDS             <1..596
                     /locus_tag="PLU55_02765"
                     /inference="COORDINATES: protein motif:HMM:NF017808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPJ87010.1"
                     /translation="YYIDTVVTDDGSFMLTSKWESIDTYASRLNDIVSNVKYITGKDK
                     VIIVAHSMGGLVTRKYIQLYGEDSLEKIVLIGIPNHGVDGFVLNYCAVFGADLECSEM
                     NKSSEFISNLNSVPLPDIPIYNLVGVGCFWEGSDGDGIVKSSSAYLDGAENIYVNGTC
                     NLVDFFHVRMVHPSLHPEIYNIVKEKIEIKEVYTSVA"
     gene            complement(578..1093)
                     /locus_tag="PLU55_02770"
     CDS             complement(578..1093)
                     /locus_tag="PLU55_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87011.1"
                     /translation="MGKEVVALVLIFALFSTGVVVLILTESKNSDSDYRGKVPYSNIG
                     PGLFYSLTPQSILDENDIKFWSKNRIVEKRFTIDTKFGQDKEQDNLFNYTISSSGDCE
                     REFDKKNYTVSVYYQSAGIGPDKYNVYTVRKCLIESVTPTKLECTGEAENQKCLYSSK
                     VDCVCYYATEV"
     gene            complement(1142..1588)
                     /locus_tag="PLU55_02775"
     CDS             complement(1142..1588)
                     /locus_tag="PLU55_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87012.1"
                     /translation="MRITKETINFNKEILFGELGAIVGIQISGNLFYKLDISADLLSY
                     LIVLGAMIGASLFWFAMRLYDKTRKQKYSEKNIIEDISYFAPGSFILTAIFYYPTLFF
                     VSKYLIEKNKLIAYITTIPQIITFIVFLIAINIYRKMIMKYYNKEL"
     gene            complement(1575..2351)
                     /locus_tag="PLU55_02780"
     CDS             complement(1575..2351)
                     /locus_tag="PLU55_02780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPJ87013.1"
                     /translation="MGLAIIIPAKNEEKTISYLLSSIKEQSIQDIKIIVSDANSKDKT
                     KEIAKKYGCEIVEGGKPGKGRNKGIENAIKQGFKLCMVIDADAILPSKYYIEQSLKEF
                     KERKLDLAGTLQKPFDTKKKIELKNTIKTCKPSKHLGYILIYSLTNFFLRLFENTKNP
                     KMGNGIFIKTKIYKKVGGFDETIEFGEDSRYAKYIVKKGYKFGILKKPKKIFTSPRRF
                     EEHGFWKMVFLYIYLDIKLLLGHKFKIGGKLKYFNKNEDN"
     gene            complement(2342..3208)
                     /locus_tag="PLU55_02785"
     CDS             complement(2342..3208)
                     /locus_tag="PLU55_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPJ87014.1"
                     /translation="MIVYSHSRLSTFEQCPLKYKLRYIDKIKPEIEKTIEAHLGSSVH
                     DTLEWIYNSVKENSEKSPTLDEIINHYIKIWKKDLTQNILIVKKQLTQADYFNKGIQF
                     LADYYQEYYPFKDGTIECEKEITIKLDENTQIKGFIDRLVYDLENNRYEIHDYKTAGT
                     LPSQEKMDQDRQLALYSIAIKELYGQDKEVLLVWHYLAYNHKIISKRTREQLENLKEE
                     TKSLIKDIQNTKVFLPNKSILCEWCEYKSICPEWNFEIQEKVSEKILSKRINPFPEFK
                     EDKEREKMDIWD"
     gene            complement(3250..4242)
                     /locus_tag="PLU55_02790"
     CDS             complement(3250..4242)
                     /locus_tag="PLU55_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87015.1"
                     /translation="MRKKFVIIVLTVFLIASMGFIIAKEKISTNVKDELIACTMDVKM
                     CPDGSYVGRIAPDCEFAPCPEKNKEKISCKDLYWIDNTNKECSQKQFCGAFMYYGLYT
                     FETKEECLKFLEDDGELIGASCGTVTPGYQNQCCIRKGYEGWDEQEFKCIGEKNRENK
                     RERIRLQDGSTQNEIECPEGCVCSGSNVKCSFENGTRVMTVHAGNSGNTIIQIKNINA
                     STNVTLYKQNEKVYGTFKDNETKEIVLPDQIMKKLKERKQKRLRLYNESIELDENGYY
                     HVEMKKKSRLFLLVPVREHVRAEVNAETGEIVKIRNPWWGFLAKDIKEDIEIED"
     gene            complement(4319..5512)
                     /gene="eif2g"
                     /locus_tag="PLU55_02795"
     CDS             complement(4319..5512)
                     /gene="eif2g"
                     /locus_tag="PLU55_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HPJ87016.1"
                     /translation="MAKSENIPEINVGIVGHIDHGKTTLLSRITGKFTDTHSEELKRG
                     ITIKIGYSEMTIYKDSEGKYNTTKGTPQRYISFIDAPGHEMLMANMLSGAATMDAALL
                     IIAANEGIKPQTREHFTALQAKGINNIIIIQNKIDLVSKEKAIENYKKIKEFVKGTIA
                     ENSPIIPVSAQQNINIDKILQELCNLEIPKRDEESKPILLVARSFDINKPGTEISKLR
                     GGVLGGILKKGKLKVGDEIEIKPGLNFKKNNQTVYQTLTTKILSIYKGKESIKEVSPG
                     ASIALETSLDPSLTKADSLNGCVISLKGTLPEITHKISIKTKLFEEVIGTEQREKVEP
                     LKNKEVLMLSVNTTITVGIIEKISKETIDVSLNIPIIALKKDNVGIARNLNNHWRLIG
                     FGEII"
     gene            complement(5545..5988)
                     /locus_tag="PLU55_02800"
     CDS             complement(5545..5988)
                     /locus_tag="PLU55_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6e family ribosomal protein"
                     /protein_id="HPJ87017.1"
                     /translation="MPFKINIGTKQGKTYKLETESEALVGKALGEIIKGKDISPSLDG
                     YEFEITGTSDKSGFTSMKDVEGIGLKKMLLGYGKGMHKRPKREGKRKRSNPTPRGLRL
                     RKTVRGKIISNAIVQINLKPTKEGPKKLSEIFQEQNKSEAPVEKN"
     gene            6113..7768
                     /locus_tag="PLU55_02805"
     CDS             6113..7768
                     /locus_tag="PLU55_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87018.1"
                     /translation="MSKSKKNIKQNYKKRLQNNFNSRTRFVRNKVSSVKPVERSISEI
                     TGVKETVSLTGYVNKIVQTGGPTVFVISDGTGNFSLKGFIKPGVRAYPEIVEGMIVRA
                     TVEIGEFQGEIEGEIKSIGILDEFNKKIFLDKMREIEKERARVTPPAFIVESAILNKL
                     KNKFIEAATQIRLAIIQDRPIIVRHHNDTDGYSSGFALERAILPLIEKQHSSEKAAWE
                     FFTRAPCAAPFYEIDDSIRDMANSLRNVAKHSNKMPLIIIADNGSSPEDLMAIKHGKV
                     HGADFIVIDHHYFNHDAISSEVLTHINPFLVGEEGSHFSAGMLCAEFARFVNPEVKNI
                     EQIPALAGFADRIDIANPELMDEYMKIASSQGYTKKLLSDISLVIDYVSSKLKFMEAR
                     EYIEVLFGEPRIKQKQLVELMAPYIRELDEKGFEIGKANVKLDKFGKLHLQFLHFEEF
                     FPGFGFFPKPGRIVGMLHDSLQIEKGISNLITVGIMESAMTIRATNESNFSVHEFIKY
                     LNQKIPEAFVEGGGHNNAGSINFLPNKQKEVFRLLKDFIKSRQ"
     gene            complement(7780..8115)
                     /locus_tag="PLU55_02810"
     CDS             complement(7780..8115)
                     /locus_tag="PLU55_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87019.1"
                     /translation="MAKKSLKIITLLALMAIPQSISINESYSPKLRKLEDVEKEEVAK
                     KEIAKKRYDLIEINETIESIERKRDFILMRLNEKNTIDSMKNEYNKRLNKINEELKEY
                     YSRRIQYKN"
     gene            8202..8918
                     /locus_tag="PLU55_02815"
     CDS             8202..8918
                     /locus_tag="PLU55_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012066483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ87020.1"
                     /translation="MKKEIDSDKAFIRLNNVAKYYEMGESVVKAVDGVSIDIKRGDFV
                     AVMGPSGSGKSTAMNLVGSLDMPTKGHIFLDGEDISSLNESELAQVRGKNIGFIFQQF
                     NLIPTLTVFENVVLPMALQGVPQYEREEKAEKILRRLDLLDRREHYPNQISGGQQQRV
                     AIARALANDPEVILADEPTGNLDTKTGLKVMDFLTELNKEGRTIVFVTHSPELAKKYA
                     HTIYWLKDGKIERVTKERKK"
     gene            8920..10128
                     /locus_tag="PLU55_02820"
     CDS             8920..10128
                     /locus_tag="PLU55_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPJ87021.1"
                     /translation="MNYFILAYRNLRKKGIRSYLTLLGICIGIFAVVSLITLSNGLKL
                     AVGSQFGVSSTELITIEGAGNGFGHPGSGQVNPLTQRDLQEIEKLSSVKAAVGRVIVA
                     GKIEFNNKVVFGYATNIPSGKDRDILYQQIEAKTAVGRLLKDSDSGKILLGYNFYANK
                     VGLGREIVPGNKVNIQGRDFEVVGIVEKKGSFIFDNIVFMNERDIESLYGSIEEWDFI
                     NVLPRDKKEIDKTKEDIEKLLRRLRDVKVGEEDFTVSTPEASLEQVNSILNAITIFII
                     IVASISIFVGAIGIINTMTTSVLERRKEIGIMKAIGATNRQIFMQFFIESGVLGLIGG
                     AIGVIFGVVLGYFGIIGINNFIGAEIQPQISIYLIFFALLGSFLIGAIAGILPAMRAA
                     KQNPVEALRG"
BASE COUNT         3118 a         1358 c         1626 g         4139 t
ORIGIN      
        1 cttactatat agatactgtt gttacagatg atggttcatt tatgcttact tctaaatggg
       61 agagtattga tacttatgct tctcgtttaa atgatattgt ttctaatgtt aaatatatta
      121 caggtaaaga taaagtaatt attgttgccc atagtatggg ggggttagtt acaagaaaat
      181 atattcaact ttatggggag gatagtttag aaaaaatagt tcttatagga atacctaatc
      241 atggagtgga tggttttgtt ttaaattatt gtgcagtttt tggtgctgat ttggaatgta
      301 gtgagatgaa taaaagtagt gaatttattt caaatttaaa tagtgttcca ttgccagata
      361 ttcctattta taatttagtt ggggttggat gtttttggga gggttctgat ggagatggaa
      421 tagttaagag ttcaagtgca tatttagatg gagctgaaaa tatttatgtt aatggaactt
      481 gtaatcttgt agattttttc catgttagga tggttcatcc aagtcttcat ccagaaatat
      541 ataatattgt taaagaaaaa atagagataa aagaggttta tacctctgtt gcataatagc
      601 acacacaatc tactttagat gaataaaggc atttttggtt ttctgcttct ccagtacatt
      661 ctagttttgt aggtgtaaca ctttcaatta aacattttct tacagtataa acattatact
      721 tatcaggacc tattcctgcg gattgatagt aaacagatac tgtgtaattt ttcttatcaa
      781 attctctttc acaatctcca gaacttgaaa tagtataatt gaataaatta tcttgttctt
      841 tgtcttgtcc aaacttagta tctattgtaa accttttttc tacaattcta ttttttgacc
      901 agaatttaat atcattttca tctagtattg attggggtgt taagctataa aataatcctg
      961 gaccaatatt agaataagga accttccctc tataatctga gtcagagttt ttggattctg
     1021 taagaatcaa tactacaacc cctgtgctga ataatgcaaa aattaaaact agtgcaacta
     1081 cttcttttcc catatatgtt taatgtaaat aaggtttaaa ttatttactt ttatattctt
     1141 attagagttc tttgttgtag tatttcatta tcatctttct atagatgttt attgcaatta
     1201 agaaaactat aaatgtaata atttggggta ttgttgttat gtatgctatt aatttgttct
     1261 tttctataag atattttgat acaaagaaaa gcgttggata atagaatatt gcagttaaga
     1321 taaatgatcc tggtgcaaaa taagatatgt cttcaattat atttttttct gaatatttct
     1381 gttttctggt tttatcatat aatctcattg caaaccagaa cagagaagct cctatcattg
     1441 ctcctagaac tattaagtat gaaagtaaat ctgcagaaat atctagttta taaaacaaat
     1501 tacctgatat ttgtatccca actattgctc ctaattctcc aaatagtatc tctttgttaa
     1561 aatttattgt ttctttagtt atcctcattt ttattgaaat attttaattt tcctcctatt
     1621 ttaaatttat gtcctaataa tagttttata tcaagataaa tatacagaaa aaccatcttc
     1681 caaaaaccgt gttcttcaaa tcttcttgga gaagtaaata ttttcttagg tttttttaaa
     1741 ataccaaatt tatatccttt ttttactatg tattttgcat atctagaatc ttctccaaat
     1801 tcaatagttt catcaaatcc tcccactttt ttgtatattt ttgttttaat gaatattcca
     1861 tttcccatct ttggattttt tgtattttca aataatctta aaaaaaagtt ggttaaacta
     1921 tatattaaga tatatccaag gtgttttgaa ggcttgcagg tttttattgt attttttaat
     1981 tctatttttt tctttgtgtc aaatggtttt tgcaatgttc ctgcaaggtc taattttctt
     2041 tctttaaact ctttgaggga ttgctcaata taatatttag aagggagtat agcatctgca
     2101 tctattacca tacatagttt aaatccttgt tttattgcat tttcaattcc tttatttctc
     2161 ccttttccag gcttcccccc ttcaacgatt tcacatccat acttttttgc tatttctttt
     2221 gttttatctt tggaatttgc atctgataca attattttaa tgtcttgtat tgattgctct
     2281 tttattgaag acaacaaata agaaatggtt ttttcttcat tttttgcagg tattattatt
     2341 gctagtccca tatatccatt ttttctcttt ctttatcttc tttgaattct ggaaatggat
     2401 ttattctttt agatagaatt ttttctgata ctttttcttg tatttcaaaa ttccattcag
     2461 gacaaattga tttatattca caccattcac agagtattga tttatttggt agaaatactt
     2521 ttgtattttg gatatctttt atgaggcttt ttgtttcttc ttttagattc tcaagttgtt
     2581 ctcttgttct tttagaaatt attttatggt tgtatgcaag gtagtgccag actaaaagaa
     2641 cttctttgtc ttgtccatat agttctttta ttgctattga atataatgca agttgcctgt
     2701 cttggtccat tttttcttgt gaaggcaaag ttcctgctgt tttgtaatca tgtatttcat
     2761 aacggttatt ttctaagtcg taaactaatc tatcaatgaa tccttttatt tgagtgtttt
     2821 catctaattt tattgttatt tctttttcac attcaattgt tccatctttg aatggataat
     2881 attcttgata ataatctgca agaaattgta tccctttatt aaaataatct gcttgagtta
     2941 attgtttttt gactattaaa atattttgtg ttaaatcttt tttccaaatt ttaatgtagt
     3001 gatttattat ttcatctagt gtaggtgatt tttcagaatt ttctttaact gaattataaa
     3061 tccattcaag agtatcatgt actgaacttc caagatgagc ttctattgtt ttttcaattt
     3121 caggttttat cttgtcaata tatcttaatt tatattttaa tggacattgc tcaaatgttg
     3181 ataatcttga atgcgaataa actatcatta gttgtaaaaa ataaagaaga ttataaagtt
     3241 ttagggtatt taatcttcaa tttctatgtc ttctttgata tcttttgcta aaaatcccca
     3301 ccacggattt cttattttaa ctatctcacc tgtttcagca ttaacttctg cacgtacatg
     3361 ttctcttaca ggaactaaca agaataatct tgatttcttt ttcatttcaa catgataata
     3421 tccattttca tctaattcaa ttgattcatt atatagacga agtctttttt gttttctttc
     3481 ttttaatttt ttcataattt ggtctggaag aactatttct tttgtttcat tgtctttgaa
     3541 tgttccatat actttttcat tttgtttgta gagtgtaaca tttgttgaag catttatgtt
     3601 ttttatttgt attattgtat ttccagaatt acctgcgtgg acagtcatta ctcttgttcc
     3661 gttttcaaaa gaacatttga catttgaacc agaacaaaca catccttctg gacattctat
     3721 ttcattttga gtagaaccat cttgtaatct tattctctct cttttatttt ctctattctt
     3781 ttcaccaatg catttgaatt cctgctcatc ccatccttca tatccttttc ttatgcaaca
     3841 ttgattttga tagcctggag ttactgttcc acatgatgct cctattaatt ctccatcatc
     3901 ttctagaaat ttcaagcatt cttcttttgt ttcaaaagtg tatagcccat aatacataaa
     3961 tgctccacag aattgttttt gagaacattc tttatttgtg ttgtctatcc aatataggtc
     4021 tttacatgaa attttttctt tatttttttc agggcatgga gcaaattcac aatctggagc
     4081 aattcttcct acataagaac catcaggaca cattttaaca tccattgtac aggcaattaa
     4141 ttcatctttt acattagtac ttattttttc ttttgcaata ataaacccca ttgatgcaat
     4201 tagaaaaact gttaatacta ttattacaaa tttttttctc atatttaata taatgaatta
     4261 ttctttataa atacttttat ggattcaaaa cttttggaac cttaaagctt aaagaagatt
     4321 aaataatttc accaaatcca attaatctcc agtgattgtt taagtttctt gcaattccta
     4381 cattgtcttt ttttaatgca attattggaa tatttaatga aacatctatt gtttctttgg
     4441 atattttttc tattatccca acagttatag ttgtgttaac agaaagcatc aaaacttctt
     4501 tgttttttaa tggttctact ttttctcttt gttctgttcc aataacttct tcgaataatt
     4561 ttgtttttat tgagattttg tgagttattt caggaagtgt tccttttagt gagattacac
     4621 atccatttaa agaatctgct tttgtaagtg aagggtcaag agaagtttcc aaagcaatac
     4681 ttgctccagg agagacttct tttattgatt cttttccttt atatattgaa agtatttttg
     4741 ttgtaagggt ttgataaact gtttgattgt ttttcttgaa atttaatcct ggttttattt
     4801 ctatttcatc tcctactttt agttttcctt tttttaatat tcctcctaat acccctcctc
     4861 ttaattttga tatttctgtt ccaggtttgt tgatatcaaa actcctggca acaagcaaga
     4921 ttggctttga ttcttcatct cttttaggaa tttctaaatt gcaaagttct tgaagtattt
     4981 tatcaatgtt tatattttgt tgagcagata caggaattat tggagagttt tcagctattg
     5041 ttccctttac aaattctttt atctttttgt aattttcaat tgctttttct tttgaaacta
     5101 aatctatttt gttttgtatt ataattatat tatttattcc tttagcttgc aaagcagtaa
     5161 aatgttctct tgtttggggt tttattcctt catttgcagc tattataagt aatgctgcat
     5221 ccattgttgc tgcacctgaa agcatatttg ccatgagcat ttcatgtcca ggagcatcaa
     5281 tgaatgaaat atatctttga ggtgttcctt ttgttgtatt gtactttcct tctgaatctt
     5341 tatagatagt catttcagaa tatcctattt ttattgtaat acctctttta agttcttcag
     5401 aatgtgtatc tgtgaatttt cctgttattc tacttagtag agttgttttc ccatggtcta
     5461 tatgtcccac tatacctaca tttatttctg gaatattttc tgattttgcc atataattca
     5521 attggagaaa gagtttttaa gagtttagtt tttttcaaca ggagcttcag atttattctg
     5581 ttcctggaaa atttcagaaa gcttttttgg accttctttt gttggtttca agtttatttg
     5641 aactattgca tttgaaatga tttttccacg tacagttttt cttaatctta atccccttgg
     5701 tgttggattt gatctttttc tttttccttc tctttttgga cgtttgtgca taccttttcc
     5761 atatcctaag agcatttttt tgagtcctat accttctaca tccttcattg aagtaaaccc
     5821 tgatttatca cttgttcctg ttatttcaaa ttcataacca tcaagagaag gggaaatatc
     5881 cttacctttg ataatttctc ctagggcttt tcctactagt gcttctgatt ctgtttccaa
     5941 cttgtaggtt tttccttgtt ttgttccaat atttattttg aatggcatga tatttagatt
     6001 gcgaaatggt ttttaaatca ttcgttttat acttatttat atacccagaa gttttttaaa
     6061 caatatatta ttaatttctt ttattaaagg taataaaagg atttaattta ttatgagtaa
     6121 gtcaaaaaag aatataaaac agaattataa aaaacgatta caaaataact ttaattctag
     6181 aacaagattt gttagaaata aagtttcttc tgttaaacct gttgaaagga gtatatctga
     6241 gataactgga gttaaagaaa cagtttcttt aactggatat gttaacaaga ttgtgcagac
     6301 aggggggcct actgtttttg ttatttcaga tggaactgga aatttttctc ttaaaggatt
     6361 tattaaacct ggagttagag catatccaga gatagttgag gggatgattg taagagctac
     6421 ggttgagata ggggagtttc agggagaaat agaaggggaa ataaaatcta ttggaatatt
     6481 agatgaattt aacaaaaaga tttttttgga taaaatgaga gagattgaaa aagaaagggc
     6541 tagagttact cctcctgctt ttattgttga gagtgctatt cttaacaaac ttaaaaataa
     6601 atttattgag gctgctactc aaataaggct tgcaataata caagataggc ctataattgt
     6661 aaggcatcat aatgatacag atggatatag ttcaggtttt gctcttgaga gagctatttt
     6721 acctttaatt gagaaacagc atagttctga aaaagctgca tgggaatttt ttacaagagc
     6781 tccctgtgct gctccattct atgaaataga cgattcaata agagatatgg caaattcttt
     6841 aagaaatgtt gcaaagcatt ctaataaaat gcctttgatt ataattgcag ataatggttc
     6901 ttctcctgaa gatttaatgg caataaaaca tggaaaggtt catggagcag attttattgt
     6961 tatagaccat cattatttta atcatgatgc tatttcaagt gaagttttaa ctcatataaa
     7021 tcctttttta gttggtgaag agggttcaca tttttctgca ggaatgctat gtgcagaatt
     7081 tgctaggttt gtaaacccag aagttaaaaa tatcgaacag atacctgctt tggcaggttt
     7141 tgcagacaga attgatattg caaatccaga attaatggat gaatatatga aaatagcttc
     7201 ttctcaaggg tatacaaaaa aattactttc agatatttct cttgttattg attatgtttc
     7261 ttctaaactt aagtttatgg aagcaagaga atatattgaa gttttatttg gtgagcctag
     7321 aattaaacag aaacaattag ttgaattaat ggcaccatat ataagagaac tagatgaaaa
     7381 aggatttgag attgggaaag caaatgttaa attagataag tttggtaaat tacatcttca
     7441 atttttacat tttgaagaat tttttccagg atttggattt ttccctaaac caggaaggat
     7501 tgttggaatg ttgcatgatt ctcttcagat tgagaaagga atttcaaatt taattactgt
     7561 tggaattatg gaatctgcaa tgacaataag ggctacaaat gaatccaatt tttcagttca
     7621 tgaatttatt aaatatctta atcagaaaat tcctgaagca tttgtagagg gaggggggca
     7681 taataatgca ggttcaatta atttcttacc taataaacaa aaagaagtat ttcgtttgct
     7741 taaagatttt ataaaatcaa gacagtgatt atttttcaat taatttttat attggattct
     7801 tcttgaataa tattctttta attcttcatt tattttattt aaccttttat tatattcatt
     7861 tttcatacta tctatagtgt ttttttcatt tagtcgcatt aatataaaat ccctttttct
     7921 ttcaattgat tctattgttt catttatttc aattaaatca tatctttttt ttgcaatttc
     7981 ttttttagct acttcttctt tctctacatc ttctagtttt cttaattttg gagaataact
     8041 ctcatttata cttattgatt gtggtattgc cattagggct aagagagtta taattttgag
     8101 tgattttttt gccatacaat ttttgtactt ttgcttgtta taaacttttt ggttattatt
     8161 tgcttggtaa agtttattaa tgatattttt attttttact tatgaaaaaa gagatagatt
     8221 ctgataaagc atttattcgc ctcaataatg ttgccaaata ttatgaaatg ggagagagtg
     8281 ttgttaaagc agtggatggt gtaagtattg atattaagag aggtgatttt gttgcagtta
     8341 tgggtccttc tggttcaggc aaatcaactg ctatgaatct tgttggaagt ttggatatgc
     8401 caacaaaagg gcatattttt cttgatgggg aagatatttc ttctttaaat gaatctgaac
     8461 tggctcaggt aagaggaaag aatattgggt ttatttttca gcaatttaat cttataccaa
     8521 cactcactgt ttttgagaat gttgtgttgc ctatggcttt gcagggagtt ccgcagtatg
     8581 agagagaaga aaaagcagaa aagattttac gtagacttga tttattagac aggagagagc
     8641 attatcctaa tcagatttca ggaggacagc aacagagagt tgcaattgca agagctttag
     8701 ctaatgaccc tgaagttatt cttgcagatg agcctacagg taatttagac actaaaacag
     8761 gattgaaagt gatggatttt cttactgaat tgaataaaga aggaagaaca attgtatttg
     8821 taacacactc tcctgaatta gctaagaaat atgcacatac tatttactgg cttaaagatg
     8881 gaaagataga aagagttact aaagaaagga agaagtaaaa tgaattattt tattcttgca
     8941 tatagaaatt tgagaaagaa gggtataagg agttatctta ctcttttagg tatttgtata
     9001 ggtatttttg cagttgtttc tttaattaca cttagtaatg ggcttaagtt ggctgtaggt
     9061 tctcagtttg gagtttcatc aactgaatta ataactattg aaggggctgg gaatggtttt
     9121 gggcatcctg gttcaggaca ggttaatcct cttactcaaa gggatttaca ggaaatagaa
     9181 aaacttagta gtgttaaagc tgcagtagga agagttatag ttgcaggaaa aattgaattt
     9241 aataataaag ttgtttttgg atatgcaaca aatattcctt ctggaaaaga tagagatatt
     9301 ctttatcaac aaattgaagc aaaaactgct gtaggtagat tattaaaaga ttcagattca
     9361 ggaaagattt tattagggta taacttttat gcaaataagg ttggtttagg tagagagatt
     9421 gttcctggaa ataaagttaa tattcaagga agagattttg aagttgtggg gattgtagag
     9481 aaaaaaggaa gttttatttt tgataatatt gtttttatga atgaacgtga tatagagagt
     9541 ttatatggtt ctattgaaga atgggatttt attaatgttc tcccaagaga taaaaaagag
     9601 atagataaaa ctaaagaaga tattgagaaa ttacttagaa gattgagaga tgttaaggtt
     9661 ggtgaagagg attttactgt ttctactcca gaagcttctt tagaacaagt taatagtatt
     9721 ttaaatgcaa ttactatatt cattattatt gttgcttcta tttctatttt tgttggagct
     9781 attggaataa tcaatactat gactacatct gttcttgaaa gaagaaaaga aattggaata
     9841 atgaaagcta ttggtgcaac aaatcgtcag atattcatgc aattctttat tgagtctggt
     9901 gttttaggat taattggggg tgcaatagga gttatttttg gagtagtcct tggatatttt
     9961 ggtattatag gaataaataa ttttattggg gcagaaattc aaccccagat aagcatttat
    10021 cttatatttt ttgctttatt ggggagtttt ttaataggag caattgcagg aattcttcct
    10081 gctatgagag cagctaaaca aaatccagtt gaggcattga gaggataaaa tgatatcaag
    10141 agaattaatt aattattctt taagaaatat aaagcaaaga aagacaagaa gtgcatttac
    10201 agtaatttca atacttgtag gaataacaac aatatttatt t
//
LOCUS       DAOPCH010000027        10217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_6364_length_10217_cov_4.546152, whole genome shotgun sequence.
ACCESSION   DAOPCH010000027 DAOPCH010000000
VERSION     DAOPCH010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 10217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10217
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6364_length_10217_cov_4.546152"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLU55_02825"
     CDS             complement(<1..298)
                     /locus_tag="PLU55_02825"
                     /inference="COORDINATES: protein motif:HMM:NF037434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPJ87022.1"
                     /translation="MTRKVLLDTNFILTCIAQKIDFFEEFYLSGIKVIIPENVISELE
                     KLKKTAALKLLEKNEFKKISLQGKNVDNSIVKFARENPEIIIATLDKEMRKKIKN"
     gene            395..529
                     /locus_tag="PLU55_02830"
     CDS             395..529
                     /locus_tag="PLU55_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87023.1"
                     /translation="MGFGDFLFGLGETAEGVKVFFMIVGLLIILPPIFLYRFVKFLMR
                     "
     gene            531..956
                     /locus_tag="PLU55_02835"
     CDS             531..956
                     /locus_tag="PLU55_02835"
                     /inference="COORDINATES: protein motif:HMM:NF022987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3232 domain-containing protein"
                     /protein_id="HPJ87024.1"
                     /translation="MEDKKIRKAMERRTQIEKILENDENGLRLLKGLTFSAWDYVNAT
                     VNFRAYISKLRDFDRCMDDLTETMVAMDLNKRTAHEALLSRLNSFNRYLFKEYPDSAP
                     LGGIYSLEPPESIKDRHSVSEWAGHYVFGIENGSKINFK"
     gene            977..1246
                     /locus_tag="PLU55_02840"
     CDS             977..1246
                     /locus_tag="PLU55_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87025.1"
                     /translation="MELTQLIKNFVSKKENADILVSLTKLSTEYYSDFINKKYSNKSD
                     VDYTHTLEKINHYLKSHKEFRNVDPRDFSIELMKYLYDHRSKPFK"
     gene            1311..1871
                     /locus_tag="PLU55_02845"
     CDS             1311..1871
                     /locus_tag="PLU55_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87026.1"
                     /translation="MQEADNVLRILKETRSALEKNDTFNLRSLSNQTINTASLTQDPD
                     NIAVAVIVYSLSKIMERLDYRQLPGWKKFYKNTLLYLDKSIQDIENKDYAKFREDFRS
                     IRGSVENLSGKLKKYVKEVLRNAEINKASRIYEHGISMEQTARLLGISQYELAEHAGK
                     TGIPDVPENRTRDTKSRIKLAMEMFE"
     gene            1871..2143
                     /locus_tag="PLU55_02850"
     CDS             1871..2143
                     /locus_tag="PLU55_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87027.1"
                     /translation="MEESILFSLIGWIGVLLIVIAYILFFTKKLKINYVLYHLLNFFG
                     SLGLIISTFLTECWPALTLFLIFAGISIAYIVKILKTKPDYKDLRN"
     gene            2145..2843
                     /locus_tag="PLU55_02855"
     CDS             2145..2843
                     /locus_tag="PLU55_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87028.1"
                     /translation="MKQLKKAIIFDAGALITISMNGLDKEIRGLKSIFDGAFLITQQV
                     KKEVIDKPLTIKSFELEALRTKKLLDDKVIELAETYGFDNKSIENKSDEIMNIANSLF
                     MSPKEPVKLLHLGEASCFALSDLLNKKNIKNVIVIDERTARMLIEKPENLRELLIRKL
                     HTNISLVKQNFAYFKNFQVIRSAELIYVAWKKDLVELKDGIFLLDALLYAVKSKGCAI
                     SSDEIEEIKKMKNF"
     gene            complement(2821..3150)
                     /locus_tag="PLU55_02860"
     CDS             complement(2821..3150)
                     /locus_tag="PLU55_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87029.1"
                     /translation="MQNVSNDKAKEILEKIGLLETTKLEKLANSFSDVKYEINPDKHN
                     PDTGSINFIYSPPGQYYRKRTSKEIAEIAEKFYDKNDYIVISTEIENGFGIIIWDNFI
                     TKSFSSS"
     gene            3261..3331
                     /locus_tag="PLU55_02865"
     tRNA            3261..3331
                     /locus_tag="PLU55_02865"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3293..3295,aa:Trp,seq:cca)
     gene            3379..3576
                     /locus_tag="PLU55_02870"
     CDS             3379..3576
                     /locus_tag="PLU55_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87030.1"
                     /translation="MIEEKVKLSNEVLLNENLNPENNKYTEDNSIERNTYTKADKSYY
                     RPHLRDIFYRFKGKHIFPDRG"
     gene            3631..3960
                     /locus_tag="PLU55_02875"
     CDS             3631..3960
                     /locus_tag="PLU55_02875"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPJ87031.1"
                     /translation="MNSALVTNNFSTYHVFFRNLANPLKIRIISALKEKESSVNDLAY
                     ELNVEQSKVSHALALLKKCNIVKYKKKGKERIYSLNKKTIIPLLKLIDEHSKSFCNCK
                     SCKGCEK"
     gene            3957..5108
                     /locus_tag="PLU55_02880"
     CDS             3957..5108
                     /locus_tag="PLU55_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPJ87032.1"
                     /translation="MRKRVYFDNASTTKVDEKVVREMLPYFSEIFGNASSQHDIGIKA
                     KDVLERSRRIIAKSIKANIGEIIFTSGGTEANNFALKGLFFSNYPQKNHIITTKIEHD
                     SILNVCRWLETQGAKITYLDVDEEGFVDIEDIRKSITDKTFLVSIIHGNNEIGTIQDI
                     EAIGKLCREKKVLFHTDACQSFTKVPINVGKQNLDLVTLNAHKIYGPKGVGALYIRDG
                     IQITPFAHGGGHEKKLRSGTENIPGIVGFAKSVDISNEKDVRRMTTLRDKLIEGLLKI
                     ENVKLNGPVGDKRLCNNINVSFNNIEGEAIGGYLENERIYTSTGSACMSNTLQTSHVL
                     KALGLSPLQSNSSLRISISKYTTEDDINYFLEKITKIVKKLRIISPLTK"
     gene            5138..5389
                     /locus_tag="PLU55_02885"
     CDS             5138..5389
                     /locus_tag="PLU55_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HPJ87033.1"
                     /translation="MKKIKITRETNMQALLFHKPELAGMLLSSGMGCMGCPMAQMETI
                     EEGCRAHGMDDKEIDKLIERLNKEESVNDKKKIKVNKRK"
     gene            5386..5838
                     /locus_tag="PLU55_02890"
     CDS             5386..5838
                     /locus_tag="PLU55_02890"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPJ87034.1"
                     /translation="MSHSLMNLFFKLVKQKYVSVGVPVIIQNSKGEILLGKRNMNMPT
                     YPGFWGLPGGMPEYGEKIENTAIREVKEELDIDIKIIKKSKNVYENLPDKKCRFHSVD
                     VPFYGKIVKGIAKPKDETKEVKWFKPSEIRNMELAYTHKEVLKGEGLI"
     gene            5841..6254
                     /locus_tag="PLU55_02895"
     CDS             5841..6254
                     /locus_tag="PLU55_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPJ87035.1"
                     /translation="MVSKYNKKVLKEFTNPKNMGEIKNANGIGKIGNPTCGDILWVYI
                     KVEKDKKGEEILTDIKFKTFGCAAAIATSSMITKLAKGKKLSEVEKISYKDVVDALGG
                     LPNIKIHCSAMASQALKKAIEDYKNKIQIKKRVNY"
     gene            6305..6958
                     /locus_tag="PLU55_02900"
     CDS             6305..6958
                     /locus_tag="PLU55_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPJ87036.1"
                     /translation="MEEEFVSKILKIGFVANDVKSFVIEKPEGYVFNSGQATNLSINT
                     LRFRDEKRPFTFTSTNNDLVIEFIVKKYNGLTKNLHELNPGDEVIIREPFGTINYFGP
                     GIFIAGGTGITPFLSIFRSLGEEIVKNNKLFFFNKTHKDIICEGELREMFNENVIFSL
                     TDEKRKGYLNKKINKSFLKKEITDFSGYFYICGSPGFVESIRKNLIELGADKNKIIF"
     gene            6975..7244
                     /locus_tag="PLU55_02905"
     CDS             6975..7244
                     /locus_tag="PLU55_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87037.1"
                     /translation="MKNKVVGWVIVGIAVVTAVVVKIFNNFLRRMIGDACTSDSDCFM
                     YNTVVIQTWVSLAIVGVILAIGIVILSWKPKKKITAKKIKKKKRN"
     gene            complement(7253..7762)
                     /locus_tag="PLU55_02910"
     CDS             complement(7253..7762)
                     /locus_tag="PLU55_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87038.1"
                     /translation="MPLKLFKHTNKDIDLFYTEEMIEEREFYDSQKRDIACWRTKQYY
                     LEKNQDYVKIAKVNSRKTGLERKAILTAHGMCIKNHWFYCNEYAGYPIQHWIDEVDGQ
                     YNVLIIDVCNDKQAKISSEKSVVIHPNESISNRKLMQDNVQFDIYIPGIGYLDSYLFE
                     EQLKQLQEK"
     gene            complement(7790..8095)
                     /locus_tag="PLU55_02915"
     CDS             complement(7790..8095)
                     /locus_tag="PLU55_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87039.1"
                     /translation="MNTKPTKKKVIGTIIISLSINVAVPLINWIITTITWPLIKKYIE
                     TNQVEGLFQPPLLTALTTYLLSPLNIAIFVVEVILIYLIWSLFQRKRTPQKLKKHNS"
     gene            complement(8097..8366)
                     /locus_tag="PLU55_02920"
     CDS             complement(8097..8366)
                     /locus_tag="PLU55_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87040.1"
                     /translation="MKEKGYSILALSAFVLGIASIFILLQLIIGQIIVIFLPVLVITL
                     GILCLQKTNSKENSEERKLAIISIILGALGITIITLMVIFGGWSY"
     gene            complement(8415..9464)
                     /locus_tag="PLU55_02925"
     CDS             complement(8415..9464)
                     /locus_tag="PLU55_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HPJ87041.1"
                     /translation="MEKKEEQEDFKFAKITLYFIIGLVIILIIVGFVKLSFNKKISKE
                     TIKKIDENIVLVQYVYGGKSMEGIYVESISSGSGVLYYGDDNEYHILTNRQIVDCGYW
                     DVGDKCWEKLWEKAIVITRDGKTHNVTSIGYSPYELNLAQLVIKRQEGEEYSTIKEIE
                     KPSLNQHVLAAGYPEYSDDTAKLSINKGQITGFKEHITKDNFSYQGIESDAYKNFKST
                     GGGLFDEKGNLLGITIWSNEYSAYAINYHNIITFLDGFSYCEEGYYFKSGNCIEKCYE
                     NQIRSIIDGLCYREVKTTCENQNYYCDEGQYCFNNQCISCPKNTYLFEDGLCYYKSAK
                     EIPYREATYHKINDT"
     gene            complement(9505..9930)
                     /locus_tag="PLU55_02930"
     CDS             complement(9505..9930)
                     /locus_tag="PLU55_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HPJ87042.1"
                     /translation="MEITLILFVILLLVGIIISYLIGKKTGVLQRDKYWEKELPNQRK
                     QAITQSRAVLGGQFSEQLSPYLPNFKYSPTECKFLGKPIDFICFKGMDEKSITEVIFV
                     EVKSGKKSNKLSPQEKNLKEAIEKKKVRWEEYRVPEDIT"
     gene            complement(9954..>10217)
                     /locus_tag="PLU55_02935"
     CDS             complement(9954..>10217)
                     /locus_tag="PLU55_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87043.1"
                     /translation="QLDCYALLFEKNKLKHPGYAYLIFFMPLEVKENGKIDFKIEVKK
                     VKVDSKRALKNFKEAIKLIKSERPKNNSECKFCSWAREEWNSK"
BASE COUNT         3608 a         1236 c         1644 g         3729 t
ORIGIN      
        1 tattttttat tttttttctc atttctttat ctagagtggc tataattatt tcaggatttt
       61 cccttgcaaa tttgactatt gaattatcta catttttacc ttgaagagat atttttttga
      121 attcattttt ttctaatagt tttaatgcag cagttttttt tagtttttcc agttcagata
      181 taacattttc agggataatt actttgattc cagataagta aaactcttca aaaaaatcta
      241 ttttttgggc tatacaagta agtatgaaat ttgtatctaa aagcactttt cttgtcatag
      301 gaaaggttag aagataatac ttataatatt ttgttatact gtgtagtagt aacataaacg
      361 aaatatttaa atagtttaaa ttggatatac aattatggga tttggagatt ttttatttgg
      421 attaggagaa actgctgaag gagttaaagt cttcttcatg atagttgggc ttttgataat
      481 tttacctcca atattcttgt ataggtttgt aaaattttta atgagataaa atggaagata
      541 aaaagatacg taaagcaatg gaaagaagga ctcaaataga aaagatattg gaaaatgatg
      601 aaaatggatt gagactatta aaaggactaa ctttttctgc ttgggattat gtgaatgcta
      661 ctgttaattt tagagcatat atctcaaaac ttcgtgattt tgacagatgt atggatgatt
      721 taacagaaac tatggtagca atggatttaa acaaaagaac agctcatgaa gctcttcttt
      781 ctagattaaa ctcattcaat aggtatcttt ttaaagagta tccagattca gctcctcttg
      841 gaggaattta ttctttagaa ccccctgaat ctataaaaga taggcattct gtttcagagt
      901 gggcaggtca ttatgttttt ggaattgaaa atgggagtaa aattaacttt aaatagggat
      961 aattagtcaa ataaaaatgg aactaacaca attaatcaaa aattttgtat caaaaaaaga
     1021 aaacgctgat atacttgtaa gtttaactaa attaagcact gaatattatt ctgattttat
     1081 aaacaaaaaa tactcaaata agagtgatgt tgattatact catactttag agaagataaa
     1141 tcattatcta aagagccata aagagttcag aaatgttgat ccaagagatt tttctattga
     1201 gcttatgaaa tatttatatg atcacagatc taaaccattt aaataagaag taaatcttaa
     1261 actttaaata atcttgtttc tattattttt tgttttaaaa ggagagttat atgcaagaag
     1321 ctgataatgt tttaagaata ttgaaagaga caaggagtgc tctggaaaag aatgatacat
     1381 ttaatcttag gagtttaagt aatcaaacaa taaacactgc ttctttaact caagacccag
     1441 ataatatagc tgttgcagta attgtttatt ctttaagtaa gattatggag agattagatt
     1501 acagacaact tcctggttgg aagaagtttt acaaaaatac tttattatat ttagataaat
     1561 caatacaaga tattgagaat aaagattatg ctaaatttcg tgaagatttt agaagtataa
     1621 gaggttctgt tgaaaatctt tcaggtaaat taaaaaaata tgttaaagag gttcttagaa
     1681 atgcagaaat taacaaagct tctagaattt atgagcatgg tatttcaatg gaacagacag
     1741 cacgtctttt aggtataagt caatatgaac ttgcagaaca tgcaggtaag acaggtattc
     1801 ctgatgttcc tgaaaaccgt actagagata ctaaatcacg aattaaactt gcaatggaga
     1861 tgtttgaata atggaagaat ctattttatt ttctttaatt gggtggattg gagttttact
     1921 tattgtaata gcttatattt tgttttttac aaagaaactt aagataaatt atgttttgta
     1981 tcatctcttg aatttttttg gttctttagg attaattata agcacatttt taactgaatg
     2041 ctggcctgct cttactttat ttttgatttt tgcaggtatt tctattgctt atattgttaa
     2101 gatacttaaa actaaacctg attataaaga tttgagaaat taaaatgaaa caactaaaaa
     2161 aagcaataat ttttgatgca ggtgcattga taacaatttc aatgaatggg ttagataaag
     2221 aaataagagg acttaaaagt atctttgatg gagctttttt gattactcag caagttaaaa
     2281 aagaagttat tgataagcct ttaactataa aaagctttga acttgaagca ttaagaacaa
     2341 aaaaactttt agatgacaag gtaatagaac ttgcagaaac ttatggtttt gacaataaaa
     2401 gtatagaaaa taaatcagat gaaataatga atattgctaa ttctcttttt atgagcccaa
     2461 aagaacctgt aaaattactt caccttggag aagcgtcttg ttttgctctt agtgatttat
     2521 tgaataagaa aaatattaag aatgttattg ttattgatga aagaactgct aggatgttaa
     2581 ttgagaaacc agaaaatcta agagaacttc ttataaggaa gttgcataca aatatctctc
     2641 ttgttaagca gaattttgca tatttcaaaa actttcaggt tatacgttct gcagaattaa
     2701 tttatgttgc ttggaaaaaa gatttagttg aattgaaaga tggaatattt ttattagatg
     2761 cacttcttta tgctgtaaaa tccaaggggt gtgctatatc ctcagatgag attgaggaga
     2821 ttaagaagat gaaaaacttt tagttataaa attatcccaa attattatcc caaatccgtt
     2881 ttcaatttct gttgaaatca ctatataatc atttttatca taaaattttt ctgctatttc
     2941 tgctatttct ttacttgttc tttttctata atattgccca gggggagagt aaataaagtt
     3001 tatagaacct gtatctggat tgtgtttatc aggatttatt tcatatttta catcactgaa
     3061 agaatttgct aatttctcaa gtttggttgt ttctaatagt ccaatttttt ctaatatttc
     3121 tttcgcttta tcatttgata cattttgcat aatattttat atggagataa tttataagaa
     3181 ttattgcatt agaaaagata ggggtgtata gtattttcct ttagaaagat taaaaaatga
     3241 actatttcta tcatgtttag gggtgcgtag gctagtggta aactggacgg ctccagaccg
     3301 tcatttgcag gttcgattcc tgccgcgccc attttatttt ataataatct ttaaatagct
     3361 tcaatcgaat ttatgtatat gatagaagaa aaagttaaac taagtaatga agttttatta
     3421 aatgaaaatt taaatcctga aaataacaaa tatactgaag ataactcaat agaaagaaac
     3481 acttatacta aggctgataa atcttattat agacctcatt taagagatat attttacagg
     3541 tttaaaggaa agcatatatt tccagataga gggtagttta atttaagtgt ttttcaaaac
     3601 atttataaat atctatgaaa tacttttcat atgaatagtg ctttagtgac taacaatttt
     3661 tcaacttatc atgtgttttt tagaaattta gcaaatccat tgaagataag aataatttct
     3721 gctttaaaag aaaaagaaag ctctgtaaat gatttggctt atgaacttaa tgttgaacag
     3781 agtaaggttt ctcatgcatt ggcattgtta aaaaaatgta atattgtgaa atataagaag
     3841 aagggtaagg agagaatcta ttcactaaat aaaaaaacaa ttattcctct gcttaaatta
     3901 attgatgagc attcaaaatc attttgcaat tgtaagtcat gtaaggggtg tgaaaaatga
     3961 gaaaaagagt atattttgat aatgcatcaa caacaaaagt tgatgaaaaa gttgtaagag
     4021 aaatgcttcc atatttttcc gaaatatttg gtaatgcatc ttcacaacat gacattggga
     4081 taaaagctaa ggatgttctt gagagaagca gaagaataat tgcgaaatca attaaagcaa
     4141 acataggaga aataattttt acatctggag gaactgaggc aaataatttt gctcttaaag
     4201 gattattttt ttcaaattat cctcaaaaaa atcatataat cacaacaaag atagaacatg
     4261 attcaatttt aaatgtttgt agatggttgg aaacccaagg agctaaaata acctatttag
     4321 atgttgatga agaagggttt gttgatattg aagatataag aaaatctatt acagataaaa
     4381 cttttttggt tagtatcata catggaaata atgagatagg gacaattcag gatatagaag
     4441 caataggaaa attatgtagg gagaaaaaag ttttatttca tactgatgct tgccaaagtt
     4501 ttacaaaagt cccaataaat gttggaaagc aaaatttgga tttagttaca ttaaatgctc
     4561 ataaaattta tggccctaag ggagttggtg ctttgtatat aagagacgga atacaaataa
     4621 ctccttttgc tcatggtgga ggacatgaaa agaaattaag aagtggtact gagaatatac
     4681 ctggaatagt tggttttgcg aaatcagttg atatctcaaa tgaaaaagat gtcagaagaa
     4741 tgactacttt aagagataaa ttgattgaag gtcttttgaa gatagagaat gtgaaattaa
     4801 atggccctgt tggagataaa agattgtgta ataatataaa tgtatctttt aataacattg
     4861 agggagaagc tattggagga tatttagaaa atgaaagaat ttatacttca actggaagtg
     4921 catgcatgtc taatacactt caaacaagtc atgttctgaa agctttagga ttaagtcctt
     4981 tacaatccaa tagttcctta agaatttcaa taagtaaata cacaactgag gacgatataa
     5041 attattttct tgaaaaaata actaaaatag ttaaaaaatt gaggattata agccctttaa
     5101 caaaataatc aattaaatat aaaattgttt aaataaaatg aaaaaaataa agattaccag
     5161 agagactaat atgcaggctt tgctttttca taaaccagag ttagctggaa tgttgctttc
     5221 atctggtatg gggtgtatgg gttgtcctat ggcacaaatg gagacaatag aagagggttg
     5281 cagagctcat ggtatggatg ataaggaaat agataaactt attgaaagat taaataaaga
     5341 agaatcagtt aatgataaaa agaaaattaa ggtaaacaaa agaaaatgag tcattcactt
     5401 atgaacttat tttttaaatt agttaaacag aaatatgttt ctgttggagt gcctgtcata
     5461 attcagaatt ctaaaggaga aattttgctt ggtaaaagga atatgaatat gcctacttat
     5521 cctggattct ggggattacc tggaggaatg cctgaatatg gagagaaaat tgaaaatact
     5581 gctataagag aagtaaaaga agaattagat attgatatta aaataattaa gaaaagtaaa
     5641 aatgtttacg agaatctacc tgacaaaaaa tgcaggtttc atagtgttga tgttccattt
     5701 tatgggaaaa tagttaaggg tattgctaaa cctaaagatg aaactaaaga agttaagtgg
     5761 tttaaacctt ctgaaattag gaatatggaa ttagcatata cacataaaga agttctgaaa
     5821 ggagagggat taatataaac ttggtatcaa aatacaataa aaaagttttg aaagaattta
     5881 caaatcctaa gaatatgggg gaaataaaaa acgctaatgg tattgggaaa attggaaatc
     5941 caacttgtgg ggatatttta tgggtatata ttaaagttga aaaagataag aaaggagaag
     6001 aaattttaac agatataaaa ttcaaaacat ttggttgtgc tgctgcaatt gcaacttcat
     6061 ccatgattac aaaacttgca aagggtaaga aattaagtga agttgaaaaa ataagttata
     6121 aggatgttgt tgatgcttta ggaggattgc ctaatattaa gattcattgt tcagctatgg
     6181 cttctcaggc attaaaaaag gctattgaag attataaaaa taaaattcaa ataaaaaaga
     6241 gggttaatta ttaaaacgat attttaatat actctccaac tctctgataa atataaacag
     6301 aaaaatggaa gaggaatttg tatccaaaat attaaagatt ggttttgttg ctaatgatgt
     6361 taaaagtttt gtaattgaaa aaccagaagg atatgtgttt aattcaggac aagcaacaaa
     6421 tctttctata aatactctta ggtttagaga tgagaaaaga ccctttacat tcacttcaac
     6481 aaacaatgat ttggttattg agtttattgt gaaaaaatat aatggtttaa caaaaaatct
     6541 tcacgaactt aatcccggcg atgaagtaat aattagggaa ccatttggca caataaatta
     6601 ttttggacct ggaattttta ttgcaggtgg aacaggcata actccttttt taagtatttt
     6661 tagaagttta ggagaggaga tagttaaaaa caataaactt tttttcttta ataaaactca
     6721 taaagatatt atttgtgaag gggaattacg tgagatgttc aatgaaaatg ttattttttc
     6781 attaacagac gagaaaagaa aaggatattt aaataaaaag ataaataaat catttctgaa
     6841 aaaagaaata actgatttta gtggatattt ttatatttgc gggtcaccag gttttgttga
     6901 gagtattaga aaaaatttaa ttgaactcgg tgctgacaaa aataaaataa ttttttgata
     6961 aaaaggagag taacatgaaa aataaagtgg ttggatgggt aattgttgga attgctgttg
     7021 taactgcagt tgtagttaag atttttaata actttttaag aagaatgatt ggagatgcat
     7081 gtacttctga ttcagattgt tttatgtata atactgttgt aattcagaca tgggtaagtt
     7141 tagcaattgt cggagttatt ttagcgatag ggattgttat cttatcttgg aaacctaaaa
     7201 agaaaataac tgctaaaaaa ataaagaaaa agaagagaaa ttaattttgt ttttattttt
     7261 cttgtaattg tttcaattgt tcttcaaata aataagaatc aagataacct atgcctggta
     7321 tatagatatc aaattgaaca ttgtcttgca ttaattttct gtttgatata ctttcatttg
     7381 gatgaattac aactgatttt tctgaactta ttttagcttg tttatcatta cacacatcaa
     7441 tgattaaaac attatattgc ccatctactt catctatcca atgttgtata ggatatcctg
     7501 catattcatt gcaataaaac caatggttct ttatacacat cccgtgtgca gttaagattg
     7561 cttttctttc aagccctgtt tttcttgaat taacttttgc tatttttaca taatcttgat
     7621 ttttttctag atagtattgt tttgttctcc agcatgcaat atctcttttt tgagagtcgt
     7681 aaaattctct ctcttctatc atttcttctg tatagaataa atcaatatct ttgtttgtgt
     7741 gtttgaataa ctttaggggc attgttataa aacttataaa aagaggtttt taagaattat
     7801 gctttttgag tttttggggt gttcttttcc tttggaataa gctccatatt agatatatta
     7861 aaattacttc aactacaaat atagctatat ttaatggcga aagaagatat gttgtaagag
     7921 cagttagaag cgggggttga aataatcctt ctacttgatt tgtttctata tattttttta
     7981 ttaatggcca ggttattgtg gttataatcc aatttatcaa tggtacagct acatttattg
     8041 agagtgagat tattatagtt cctattactt tcttttttgt tggttttgta ttcactctag
     8101 taactccaac ctccgaagat taccattaat gtgattatgg ttatccctaa tgcaccgagt
     8161 ataatagaga ttattgctaa tttcctttct tctgaatttt cttttgaatt ggttttttgt
     8221 aaacacagaa tcccaagagt tataactaaa acaggtagaa aaattacaat tatctgtccg
     8281 attatcaatt gtaatagaat aaatatactt gctattccta atacaaaagc tgataatgct
     8341 aaaatagaat accctttttc tttcattttt attcctcttt tgattaaata gagaatcctt
     8401 atttgttttt aaaattatgt atcatttatc ttgtggtatg ttgcctctct gtatggaatt
     8461 tcttttgctg atttgtaata acataaacca tcttcaaata aataagtatt tttagggcaa
     8521 cttatacact gattgttgaa acaatactgt ccttcatcgc aataatagtt ttggttttcg
     8581 caagttgttt tgacttctct ataacataat ccatctatta tgcttcttat ttgattttca
     8641 taacactttt caatacaatt tcctgattta aagtaatatc cttcttcgca ataagaaaaa
     8701 ccatctaaaa atgttattat attatgataa tttattgcat atgcagaata ttcatttgac
     8761 caaatagtta tgcctaagag atttcctttt tcatcaaata atcctcctcc tgtgcttttg
     8821 aaatttttat atgcatctga ttctattccc tgataagaaa aattatcttt tgtaatgtgt
     8881 tctttgaatc cagttatctg tcccttattt attgataatt ttgctgtgtc atctgagtat
     8941 tctgggtatc ctgcagctaa gacatgctga ttaagggaag gtttttctat ttctttaata
     9001 gttgaatact cttctccttc ttgtctttta attactaatt gagctagatt taattcatat
     9061 ggggaataac caatacttgt tacgttatgg gtttttccgt ctcttgttat tacaattgct
     9121 ttctcccata gtttctccca gcacttatct cctacatccc aatatccaca atcaacaatt
     9181 tgtctgtttg tgagtatatg atattcatta tcgtctccgt aatataaaac ccccgaacca
     9241 cttgatattg attcaacgta aatcccttcc atggattttc caccgtaaac atattgtact
     9301 aatacaatgt tttcatctat ttttttaatt gtttctttac ttattttttt gttgaaacta
     9361 agttttacaa atccaacaat aattagaatt ataactaatc cgattatgaa gtaaagtgtt
     9421 atttttgcaa acttaaaatc ttcctgttcc tctttttttt ccatattgaa ttatgtaata
     9481 cattatataa aaacttattt ggatttaagt tatatcttct ggtactctat attcttccca
     9541 ccttactttc tttttttcaa ttgcttcctt aagattcttt tcttgtggag agagtttgtt
     9601 ggatttttta ccgcttttta cttctacaaa tataacttct gttatacttt tttcatccat
     9661 tcccttaaaa caaataaaat ctattggctt tcctaggaat ttgcattctg ttggtgaata
     9721 tttgaaatta ggtaaataag gagagagttg ttctgagaat tgccctccaa gaaccgctct
     9781 tgattgagtt attgcttgtt ttctttgatt gggtaattct ttttcccaat atttatctct
     9841 ttgaagaact cctgtttttt taccaattag atatgagatt attattccaa ctagtaacaa
     9901 gattacaaag agtattaatg ttatttccat tttgctttaa ttttataatt aaattatttt
     9961 gaattccatt cttctcttgc ccaagagcaa aatttacatt cagaattgtt ttttggtctt
    10021 tcacttttta ttagttttat tgcttctttg aagtttttta atgctctttt actatctacc
    10081 ttaacttttt tgacttctat tttaaaatct atttttccat tttcttttac ttccaagggc
    10141 atgaagaata ttaaataagc atacccagga tgttttaatt tatttttttc aaaaagcaat
    10201 gcgtaacaat ctaattg
//
LOCUS       DAOPCH010000028        10167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_6392_length_10167_cov_4.597211, whole genome shotgun sequence.
ACCESSION   DAOPCH010000028 DAOPCH010000000
VERSION     DAOPCH010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 10167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10167
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6392_length_10167_cov_4.597211"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PLU55_02940"
     CDS             <1..610
                     /locus_tag="PLU55_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPJ87044.1"
                     /translation="IYYSDGLSRNNTLLNCSYDNEFFTGSSGEIIRKWYYQAYTNYSN
                     GSAVAGANITAYNTTGNIQFTKQTNSSGYIPRQEITEYVNRGGIRSYYNNYTINASKS
                     GYITDNNLFNFTMTRNKVDDFFTLFLSTCNYSSGNWLINCSENCYIDSNYNIGKNNIS
                     IIGVGTVTLSGNISNYNKLLIAGQDSSNICTVRCINGGCFKF"
     gene            complement(600..917)
                     /locus_tag="PLU55_02945"
     CDS             complement(600..917)
                     /locus_tag="PLU55_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87045.1"
                     /translation="MKLKKKSPEDYRKKSKRPAETKNISLERIARGIVTASSPVLFQV
                     ELSRKRYHTFTKDDASEYNQPRLKTSIRCIYYGHRNNLLPYAIYDLNWNLIWKDKNEK
                     TKI"
     gene            993..1493
                     /locus_tag="PLU55_02950"
     CDS             993..1493
                     /locus_tag="PLU55_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87046.1"
                     /translation="MNFQFYFEKLVGSKDFKKFISENKDAFFCSAFFAVDKKGKDNKQ
                     NIDYYVPSINKMFSFKINDGVELIPVEDYGEVFKPEKIPDNLDFDFNHIEKLIEGEMF
                     EKKIKNKIEKLLLSIQAKEKKVYILGTIFISGLSLIKIKIDLDEKKIVDFEKMSFFDM
                     IKVIKK"
     gene            complement(1502..1738)
                     /locus_tag="PLU55_02955"
     CDS             complement(1502..1738)
                     /locus_tag="PLU55_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87047.1"
                     /translation="MKLNKKVSDLARKLEFIGIDEELKRREIIKSCIGLPGLITGFYL
                     VKKINGSYARLKKEVDNYNKLKYLEQRTQRIIYN"
     gene            complement(1779..2378)
                     /locus_tag="PLU55_02960"
     CDS             complement(1779..2378)
                     /locus_tag="PLU55_02960"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPJ87048.1"
                     /translation="MENQQKVWNNIAEEWQKFKETPSELSQQFLKSCTGKVLDLGSGT
                     GRHLTKINNGKMYLIDFSEEMINLAKKKATKLKIPAEFIVADISKELPFQDNFFDYAI
                     SISAIHCLQPQNHKKTISELYRVMKPKSKVLIGVWNFKSKRFNQKKGKEKMIKWTDKG
                     ERYYYLFEEEEIHNLFKSVGFKIVSTHNSEMMINFIVEK"
     gene            complement(2471..3022)
                     /locus_tag="PLU55_02965"
     CDS             complement(2471..3022)
                     /locus_tag="PLU55_02965"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HPJ87049.1"
                     /translation="MNSFSREQEINKLKEKVRTIPDFPKQGVMFRDITTLLQNKESLR
                     KLVELFYEKYKDVEIDIVAGIESSGFIMAGLLAEKLNAGLVLIRKPGKIPYETIKQNY
                     KLEYGEGSVEIPKDAIKPGQKVLIIDDLIAKGGTVQAVADLIEKMNGVIAGCAFVIEL
                     PELKGRDKLSKYPFFSLIKFEGE"
     gene            complement(3029..3748)
                     /gene="mtnP"
                     /locus_tag="PLU55_02970"
                     /pseudo
     CDS             complement(3029..3748)
                     /gene="mtnP"
                     /locus_tag="PLU55_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006917735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
     gene            3864..5243
                     /locus_tag="PLU55_02975"
     CDS             3864..5243
                     /locus_tag="PLU55_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HPJ87050.1"
                     /translation="MKTPTNNEVRKPTKTISGITPVAVMLPPKKCKHGNCIYCPSMNV
                     PQSYTPKSPVVLRAKSLDYDSYKQVVSRIKAFEVMNHPTDKIELIIMGGTFLEYPEKF
                     QYEFIKGLYDGLNGKISKNLSEAKKINENSKHRCVALCIETRPDVCCEFIERMREFGC
                     TRVELGVQLIDDKVYKLVKRGHSIKEVILATKCLRNAGFKIGYHLMPGLPGSNLKKDL
                     ELFKKIFSDENFKPDQIKIYPCQVLPNSELENLYWEGKYKPYTPKEIQKLLVDMLKIV
                     PRYCRVMRIMREIPLEYLIAGSKDIGIRKDVEEDLRKNNVKLKEIRYREIGFNKSAGN
                     LDTNLKLKKSEYKASSGKEYFLEIVNKKDILFGLLRLRIFNKKAMIRELHVYGKSLKI
                     GEGAKEKGQQHTGLGKWLMDSAEKLAKKEKCKEIKVISGVGVREYYRKLGYVLQEGKG
                     NGEYMRKEI"
     gene            complement(5251..6087)
                     /locus_tag="PLU55_02980"
     CDS             complement(5251..6087)
                     /locus_tag="PLU55_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPJ87051.1"
                     /translation="MRPRYSFSSRHTGKIRNIRKQRGKYPSIAKKVVEDSDVILEILD
                     ARFIKETRNKEIEKYIKHKNKRIIYVLNKSDLINKSNLRIPDEMFPYVMVSATNRKGI
                     KELRDRIKIEAKKVEKNPEDYHKVIVGIIGYPNTGKSSLINSLVGKSSAGVGSDAGFT
                     KGMQKVKLSQNIVLMDSPGIIPESEYSSQDREKVSRHTKVGARSYSQIKEPDIVINLI
                     MKEHPGILEKHYKIDAKGNPETLLEELGKRKGFLRKGGEVNDDAVARLILKDWQEGKI
                     KL"
     gene            complement(6204..6803)
                     /locus_tag="PLU55_02985"
     CDS             complement(6204..6803)
                     /locus_tag="PLU55_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87052.1"
                     /translation="MDDYLERLKGMKRKKVVDEDYKQGYKQTIHAIKNPQDLGKDKKL
                     LETGKNFVKYFNGEKKSPSDWRKGNSSKIASELMTRGIKNRNLEYSLVGLKLYDCIGL
                     ADRDSVKRKFERAAKLNPNPKYLNQGIEIFEKYKNDFGIEQKVLGFLSIAFFLAGISF
                     LSLNLTGNVIGNMTRSSGNILGIILFVLGIVGLFLVIKE"
     gene            complement(6906..7226)
                     /locus_tag="PLU55_02990"
     CDS             complement(6906..7226)
                     /locus_tag="PLU55_02990"
                     /inference="COORDINATES: protein motif:HMM:NF018476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family
                     toxin"
                     /protein_id="HPJ87053.1"
                     /translation="MECKVKFIDLELKHAFEDLEKSDPRLFKEVNKAIQDICQNSFCG
                     RNVKKKLIPKELVQKHKIDNLWIYNLRKDWRLLYSVGRDEIEIIAVILDWMDHKDYEK
                     LFKF"
     gene            complement(7211..7522)
                     /locus_tag="PLU55_02995"
     CDS             complement(7211..7522)
                     /locus_tag="PLU55_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87054.1"
                     /translation="METRTIKKNKSYIETNDIARSPTLQTVLMVEKFIKDNSGEYKKT
                     DLFKNLPKKVMWQTFQVIMEYLEENLRIAYDNEGYIVYIWNPEFVERFRNKPELEWNA
                     K"
     gene            complement(7635..>10167)
                     /locus_tag="PLU55_03000"
     CDS             complement(7635..>10167)
                     /locus_tag="PLU55_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87055.1"
                     /translation="NIINLTINCEAGGPYQSGALVLAQGNVSDGTSHLSSQIINASVY
                     LNGILNTSNNLNSTSEGSFSTIFLGLATGNYTLNVSVNYRGLNAYCLDYFSINEGYDA
                     GFISGNESGYILGFNEGNITGFNLGNTTGFVFGNSSGFTLGNVTGFILGNTTGFILGN
                     TTGYVLGNATGFNLGNTTGFILGNSTGFITGNLSGYVLGFDTGNLTGFNLGNYTGYLL
                     GNLTGFNLGNETGYTTGYAAGLTAVGNTSASLILDKIISFSSFTNQTFVYNVTLRLTN
                     KGGSNATYVNITDLDSLSSPYFIGNITPGETVVRNYLLSFARNSTTYNNITKIAQVSG
                     IDSFLSSLIYSNSSQIVLTIPGQETGQKLTLIKNVYYNSENSTNVNYTITVEVVNSGG
                     IDLKNINILDSDLNLNTLISLNRTQNYSYSNFVLVDKAASNTNKLFVKSTALANSITY
                     ESNQMQVRIPGYGGPADTIVNSPSYVNQSEDFNTTIQITNQNQDIGQNFIIYYWITNE
                     FENVNYSSGTRTIYVGASQTNNTIVTFSAPSDIGIYKFKSVTTYEGGVDLAEDSFQVV
                     APGIVINPSSGKGSSKCNYIWECSSWSVCSSEGLQTRICTNKGTCKGTVGKPIEQRNC
                     SEVSFNVKLRLNQLNLTENETLEFNITITQKKPQEEINVQVNYLVLDADENEIFNEIT
                     NYSVKDTMTYKEILNDLKLVPGEYTLKIYTIYNGEKKAEDEQRFIVREYEQGEIHSRL
                     YIQLKNRLSLFNYIVLLGIILILVLLIVIVIKIKLRRRDEIDVLIKEGKKYFYEGKFD
                     EAIDIYPVLKNLYKSKYKGDASAYEKINSYSKFLSKGVKDKLKKD"
BASE COUNT         3442 a         1517 c         1278 g         3930 t
ORIGIN      
        1 tatttattat tcagatggtt taagtaggaa taacactctt cttaattgtt cctatgataa
       61 tgaatttttt acagggagtt caggagaaat tataagaaaa tggtattatc aagcatacac
      121 taattattct aatggaagtg cagttgcagg ggcaaatata actgcttaca atacaactgg
      181 taatattcaa tttacaaagc agacaaatag ttcaggttat atcccaagac aagaaattac
      241 tgaatatgta aacagaggag gaataagaag ttactataac aattatacaa taaatgcaag
      301 caagtcagga tatataacag acaataattt atttaatttt acaatgactc gaaataaagt
      361 ggatgatttt ttcaccttat ttttaagcac ttgtaattac tcttctggaa attggctaat
      421 aaattgctct gaaaattgtt atattgactc aaactataat attggaaaaa acaatatatc
      481 tattataggg gtcgggacag ttactttatc tggaaatatt tctaattaca ataagttact
      541 tattgcaggt caagattcaa gtaatatttg tactgtaaga tgtattaatg gaggatgttt
      601 taaattttag ttttttcatt tttatctttc caaattagat tccaatttaa atcataaatt
      661 gcataaggaa gaagattgtt tctgtgtcca taatagatgc atcttatact tgtttttaat
      721 cttggttggt tgtattctga agcatcatct tttgtaaatg tgtgatatct tttacgagat
      781 aattctactt gaaataaaac aggactagat gctgttacaa tcccccttgc aattctctct
      841 aatgaaatgt tttttgtttc tgctggcctt tttgactttt ttctataatc ctctggagat
      901 tttttcttta atttcatatt tataatatat gatttatctt taaaataatt tatattctaa
      961 aaccttataa attagaatat actgaaagtt ttatgaattt ccaattttat tttgagaagt
     1021 tagtaggttc aaaagatttc aagaaattta ttagtgagaa caaagatgct tttttttgtt
     1081 ctgctttctt tgcagttgat aagaagggca aagataataa acaaaatata gattattatg
     1141 ttccttcaat caacaagatg tttagtttta aaataaatga cggagtggaa ttaatacctg
     1201 tagaagatta tggagaagta ttcaaaccag aaaagattcc agataacctt gattttgatt
     1261 ttaatcatat tgaaaaactt attgaaggtg agatgtttga gaaaaaaata aaaaataaaa
     1321 tagagaagct tcttcttagt atccaggcaa aagaaaaaaa agtttatatt ttaggaacaa
     1381 tttttatatc tggtttgagt cttattaaaa ttaaaataga tttagatgag aaaaagattg
     1441 ttgattttga aaagatgagc ttttttgata tgataaaagt tattaaaaaa tagttaattt
     1501 attaattata aattattctt tgtgttcttt gttccaaata tttaagctta ttataattat
     1561 caacttcttt ttttaatctt gcataacttc cattaatttt tttgactaaa tagaatcctg
     1621 ttattaatcc aggtaaacca atacaacttt ttattatttc tcttcttttt aattcttcat
     1681 caatacctat aaactctaat tttcttgcta agtcagatac tttcttgttt aatttcatga
     1741 attttgaatt atttatttga tatataaaga ttattatctt atttctctac aataaaatta
     1801 atcatcattt ctgaattgtg tgtagaaaca attttgaaac ctactgattt gaaaagatta
     1861 tgtatttctt cttcttcaaa taaataataa tacctttctc ctttatctgt ccactttatc
     1921 attttttctt ttcctttttt ctggttgaat cttttagatt taaaattcca aactccaatc
     1981 agtactttag attttggttt cataactcga tataattcag atattgtttt cttatgattc
     2041 tgtggttgga gacaatgaat tgcagaaata gaaatagcat agtcaaaaaa attatcctga
     2101 aaagggagtt ctttggaaat atctgcaaca ataaattctg caggtatctt aagtttagtt
     2161 gctttctttt ttgcaagatt tatcatttct tcagaaaaat caattaaata catttttcca
     2221 ttatttattt ttgttagatg tcttccagtc ccgcttccta aatccaatac cttgcctgtg
     2281 caagatttta aaaattgttg gctaagctca gatggtgtct ccttaaattt ttgccattct
     2341 tctgctatat tgttccaaac tttttgttgg ttttccataa ttaatattat tagagtaata
     2401 ttaaaaatta tttggtttta atataatata gtagttccta aatatgtttt aattctctac
     2461 aaaataaaca ttattcacct tcaaatttaa tcaaactgaa aaaaggatat ttagaaagtt
     2521 tgtcgcgccc ttttaattct ggaagctcta ttacaaatgc acaccctgct ataactccat
     2581 tcattttctc tattaaatct gcaactgcct gaactgttcc tccttttgca attaaatcat
     2641 ctataataag tactttttgt cctggcttta ttgcatcttt aggtatttcg acagaacctt
     2701 ctccatactc taatttataa ttttgtttta ttgtttcata aggaatcttc cctggttttc
     2761 ttattaaaac aagtcctgca tttaattttt ctgcaagtaa acctgccatt ataaaaccac
     2821 tagattcaat tcctgcaact atatctattt ctacatcttt atatttctca taaaaaagtt
     2881 ctacaagttt ccttaaactt tctttatttt gtaaaagagt tgttatatct ctaaacataa
     2941 ccccctgttt tgggaaatct ggaattgttc taactttttc ttttagcttg tttatttctt
     3001 gttctcttga aaatgaattt attgtgtgaa ctaaaacgtt ttttaatttt tcagtgtttt
     3061 gtttcataat ttcttttatt tcataccatg ttactggttt ctcatcttct ttccaacaat
     3121 cataatctgt tgaagttgca attgttgcaa aaggtatttc tgcttcattt gctagaatag
     3181 cttctggaga agttgacata tttattacat gtgctcccca ctgtctaaat aaattacttt
     3241 ctgcttttgt tgaaaacctt ggaccctcta ttgttataac tgttcctttt ttatgtactg
     3301 gaaattggag ttcttcacaa ctctcaatta gcttttttct taaaacttct gaaaatggat
     3361 ttgccatagg agtatgtatt ggtccaaatt caaatttatc aaaaaaagtt agttttctgt
     3421 gttttgtaaa atcaataaat tggtctaata tcataaaatc ccctcgttta atttcttcgc
     3481 gtaaagaacc tacagcagtt gttgaaatta taaacttgca tccttctgat tttaatgcaa
     3541 agatgtttgc cctgttattt acttcagttg gaggaatttc gtgttttaac ccatgccttg
     3601 agattatgaa aacatctaca ctccctattt ttcctattgt taaaggagaa gagggtttcc
     3661 caaaaggagt ttcaacttct tttgttttaa attcagtaaa aatttctgga ttttcaagtc
     3721 ccgaaccacc tattatgcct attttcatta ttactctaat tcagaaagat tttataagta
     3781 ttttgagggt tgagaatata aattaaattt cgtctttttt tacaatatgc ataatttaaa
     3841 aataactttt ttcttgaaga tatatgaaaa ctccaacaaa taatgaagtt cgtaaaccaa
     3901 caaagacaat atcagggata acccctgttg ctgttatgtt gcctccaaaa aaatgcaaac
     3961 atggtaactg catatattgc ccttcaatga atgttccaca aagctatact ccaaaaagtc
     4021 ctgttgtttt aagagctaaa tctttggatt atgattctta taaacaagtt gtttcaagaa
     4081 ttaaggcatt tgaagttatg aatcatccta cagataaaat agagctaata attatgggag
     4141 gaacattttt agagtatcct gaaaaatttc aatatgaatt tattaaagga ctttatgatg
     4201 gattaaatgg aaagatttct aaaaatttaa gtgaagctaa aaaaataaat gaaaattcaa
     4261 aacatagatg tgttgctctt tgcattgaaa cacggcctga tgtatgttgc gaatttattg
     4321 aaagaatgag ggaatttgga tgcacaagag ttgaattggg agttcaattg atagatgaca
     4381 aagtgtataa actagttaaa agagggcatt caattaaaga agttatttta gctacaaagt
     4441 gtttaagaaa tgctggtttc aaaataggat atcatttaat gccaggatta cctggaagca
     4501 atttaaagaa agatttagag ttatttaaaa aaatattctc agatgaaaac tttaaaccag
     4561 atcagattaa aatatatcct tgtcaggttt tacctaattc agaattagaa aatctttatt
     4621 gggaaggaaa gtataaacct tatactccaa aagaaattca gaagcttctt gtggatatgt
     4681 tgaagattgt tccaagatat tgtagagtaa tgagaattat gagagaaatt ccattagagt
     4741 atttaattgc aggtagcaaa gatattggaa taagaaaaga tgttgaagaa gatttaagaa
     4801 aaaataatgt taaacttaaa gaaataagat atagagaaat tggattcaat aaatctgcag
     4861 gaaatttaga cacgaatctg aaacttaaaa aatcagagta taaagcttct agtggaaaag
     4921 aatattttct agagattgtg aataaaaaag atattttatt tgggttatta agattaagaa
     4981 tattcaataa aaaagcaatg ataagagaat tacatgttta tggaaagagc ttaaaaattg
     5041 gagaaggagc aaaagaaaaa ggacaacaac acacaggatt aggaaagtgg ttaatggatt
     5101 ctgcagaaaa actcgctaaa aaagaaaaat gcaaagaaat taaagttatt tctggagttg
     5161 gagttagaga gtattataga aaattaggat atgttctgca agaaggaaaa gggaatggag
     5221 aatatatgag aaaagaaatt tagttaaaaa ttataattta atttttcctt cttgccaatc
     5281 ttttaatatt aatcttgcaa ctgcatcatc atttacttcc cctccttttc ttagaaatcc
     5341 ttttcttttt cctaattctt ctaagagggt ttctgggttt cctttagcat ctattttata
     5401 atgtttttcc aatattcctg gatgttcttt cattattaaa tttataacta tatctggctc
     5461 ttttatctgg ctatagcttc ttgccccaac ttttgtatga cgagatactt tttctctatc
     5521 ttgtgaggag tattcgcttt caggaattat ccctggagaa tccataagaa caatattttg
     5581 agagagtttt actttttgca ttccttttgt aaacccagca tcagaaccaa ctcctgcaga
     5641 actttttcca acaagtgaat taattaaaga actttttcct gtgtttggat aaccaattat
     5701 tccaacaatc actttgtgat aatcttctgg atttttttca actttttttg cttcaatttt
     5761 tatcctatct cttaattctt ttattccttt tctatttgtt gcagaaacca taacataagg
     5821 aaacatttca tctggtatcc tcaaattact tttgttaatt aaatcagatt tattcaaaac
     5881 ataaataatt cttttgtttt tatgtttaat atatttttca atttctttat tcctagtttc
     5941 ctttatgaat cttgcatcaa gaatttccaa gattacatct gagtcctcaa caaccttttt
     6001 tgcaattgaa ggatatttgc ctctttgttt ccttatattt cttatttttc cggtatgtct
     6061 tgaagaaaaa ctatatcttg gtctcatatt aaatataagt aaaaggggtt taaaaaaatg
     6121 ctgtaaatat tatctggtaa aataaaataa aggtttaacc agatatagaa ttaataaaaa
     6181 gagataatta gtagattaaa tttttattct ttaattacaa gaaataaccc aacaattcct
     6241 aaaacaaaca atattatccc caagatattc ccagaactcc ttgtcatatt cccaataaca
     6301 tttccagtca aattaaggga taaaaaagat attcctgcaa gaaagaatgc aatagataaa
     6361 aaacctagaa ctttttgttc aattccaaaa tcatttttat atttttcaaa aatctcaatt
     6421 ccttgattta aatattttgg attaggattt aatttagctg ctctttcaaa tttccttttt
     6481 acagaatctc tatcagccaa accaatgcaa tcatataatt ttagcccaac aagagaatat
     6541 tctaaattcc tattttttat tcctctagtc attaattctg aagcaatttt tgaagaatta
     6601 ccttttctcc aatcacttgg agattttttt tctccattaa aatattttac aaaatttttt
     6661 cctgtttcaa gaagtttttt atctttccct aaatcctggg gattttttat agcatgaatt
     6721 gtttgtttat aaccttgttt ataatcctca tcaacaactt tttttctttt cataccttta
     6781 agtctttcta aataatcatc catctgtaaa tataattaaa cttcttttta tacttttcca
     6841 aacaaagtta catccaagcc gtaaattcaa ccttaagttt aacttagtgt aagattaata
     6901 taaaattaaa atttgaataa tttttcataa tctttatgat ccatccaatc aagaataact
     6961 gctataattt caatttcatc tctaccaaca gaatataaga gtctccagtc ttttcttaaa
     7021 ttataaatcc agagattatc tattttatgc ttttgaacaa gttccttagg aattaatttt
     7081 ttctttacat ttcttccaca aaaagaattt tggcatatat cttgtattgc tttatttacc
     7141 tctttaaaga gtcttggatc tgatttttct aaatcttcaa aagcatgttt caattctaaa
     7201 tcaataaact ttactttgca ttccattcta actcgggttt atttctaaat ctctctacaa
     7261 attctggatt ccagatataa acaatatatc cttcattatc ataagctatt ctcaaattct
     7321 cttctaaata ctccattata acctgaaatg tctgccacat cacttttttt ggtagatttt
     7381 tgaataaatc tgtttttttg tattctcctg aattatcctt tatgaatttt tcaaccataa
     7441 gaactgtttg aagcgttggg cttctagcta tatcgtttgt ttcaatataa cttttatttt
     7501 tcttaattgt ccgagtttcc attttaatta agcttcaaaa tatcaataat atcatttgat
     7561 attactattt aaatgtttct tttagaactg taaattcaac attaggttta acttagtgta
     7621 agattaatag gaacctaatc tttttttaat ttatctttaa ctccctttga aagaaactta
     7681 gaataagagt taatcttctc ataagcagaa gcatctcctt tatattttga cttatataag
     7741 ttcttaagaa caggatatat atctattgct tcatcaaatt ttccctcata gaaatatttt
     7801 tttccttctt taattaaaac atcaatctca tctcttcttc tcaattttat cttaataact
     7861 atgacaatca agagaactaa tataagtata attccgagta atacaatata attaaataaa
     7921 gataatctgt ttttcaattg aatatataat ctagaatgaa tctctccttg ttcatattct
     7981 cttactataa acctttgttc atcttctgct ttcttttctc cattatatat tgtataaatt
     8041 tttaatgtat actctcctgg gactaatttt aagtcattta atatctcttt ataagtcata
     8101 gtatctttaa cagaataatt tgttatctca ttgaatattt cgttttcatc agcatctaat
     8161 accaaatagt taacttgtac atttatttct tcttgaggtt tcttctgagt tatagtaata
     8221 ttaaactcta aagtttcgtt ttcagttaaa tttaattgat ttaatcttaa cttaacatta
     8281 aatgaaactt ctgaacaatt tctttgttct attggctttc ctactgtccc cttacatgtt
     8341 cctttattag tgcatatcct tgtttgtaat ccctcagaag aacaaacgct ccaagaacta
     8401 cattcccata tatagttaca ttttgaactt cctttcccac ttgatggatt aattactatt
     8461 cctggtgcaa caacttgaaa gctatcttca gctaaatcta cacctccttc atatgttgtt
     8521 actgatttaa atttataaat tccaatatca gaaggtgctg aaaaagttac tatagtatta
     8581 tttgtctgag aggcaccaac ataaattgtt cttgtaccag aagaataatt tacattctca
     8641 aattcattag ttatccaata ataaataata aaattctgtc ctatatcttg attttgatta
     8701 gttatttgaa ttgttgtatt aaaatcttca gattgattta cataagaagg tgaattaact
     8761 atagtgtctg caggtccacc atatcctgga atccttactt gcatttgatt tgattcataa
     8821 gttattgaat tcgctaatgc tgttgatttt acaaataatt tattggtatt ggaagcagct
     8881 ttatccacta aaacaaaatt agagtaagaa taattttgag ttctatttaa actaattaag
     8941 gtgtttaaat ttaaatcaga atctagaata tttatatttt ttaaatcaat tcctccagaa
     9001 ttaacaacct ctacagttat tgtatagttt acatttgttg aattttctga attataatat
     9061 acatttttta ttaaagttaa tttctgacct gtttcctgtc ctggaattgt taaaactatc
     9121 tgactagaat tagaataaat caaacttgac aaaaaagaat caattccaga tacttgtgca
     9181 atttttgtaa tattgttata agtagtagaa tttcttgcaa aactaagtaa ataattcctt
     9241 acaacagttt ctcctggagt aatatttcct ataaaatatg gactagataa agaatctaaa
     9301 tctgtaatat taacatatgt tgcattgctt cctcctttgt tagtcaatct taaagttaca
     9361 ttatagacaa aagtttgatt tgtaaaacta ctaaaactta tgattttatc taaaattaaa
     9421 ctagcagaag tatttcctac tgcagtcaaa cctgcagcat aaccagttgt atatccagtt
     9481 tcattaccta aattaaatcc agttagattt ccaagcaaat atcctgtgta attacctaaa
     9541 ttaaatccag ttagatttcc agtatcaaat cctaaaacat aaccacttaa atttcctgta
     9601 ataaatcctg ttgagtttcc taatatgaaa cctgttgtat ttcctaaatt aaatccagtt
     9661 gcatttccca aaacatatcc tgttgtattg cccaatataa aacctgttgt attacctaaa
     9721 ataaatccag ttacattccc taatgtaaat ccagaactat taccaaatac aaaacctgtt
     9781 gtatttccta aattaaatcc agttatgttc ccttcattaa atcctaaaat atatccactt
     9841 tcgtttcctg aaataaatcc agcatcataa ccttcattta tgctgaaata atccaaacaa
     9901 taagcattta atccccgata attaacagat acatttaaag tataatttcc agttgctaat
     9961 cctaaaaata ttgtagaaaa actcccctca cttgttgaat taaggttatt tgaagtattt
    10021 aaaataccat ttagataaac acttgcatta attatttgag aggataaatg agaagttcca
    10081 tcacttacat ttccttgagc taatactaaa gcccctgatt gataaggccc tcctgcttca
    10141 caatttattg tcaaattaat gatattg
//
LOCUS       DAOPCH010000029        10017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_6473_length_10017_cov_3.526802, whole genome shotgun sequence.
ACCESSION   DAOPCH010000029 DAOPCH010000000
VERSION     DAOPCH010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 10017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10017
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6473_length_10017_cov_3.526802"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2658
                     /locus_tag="PLU55_03005"
     CDS             <1..2658
                     /locus_tag="PLU55_03005"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPJ87056.1"
                     /translation="GGSSGDGSSGGSPSCNDTCASLVKQCGTHIICGESVNCGNCSTG
                     YVCSLGLCIPNVCTPNCVGKNCGSDGCGGSCGTCSTGYTCQINGTCLKDDVNLRYIVK
                     NGVGYADIIVASNAPNIVKLAADYFQIYIYNMTGVRLPISNSVNQSKDYHIYIGRSSY
                     TDNLGINSQGCENDGFKMVSGANYLALIGDDAPFVLSGPDNYPEWDSLTGKKWANDYI
                     DYYGKQYNGLKDIWMTDGKGSLNAVFEFLYNQGIRWYAPGEIGTIIPEKSSIGFGDMN
                     VLINPDVPMRSMFHYYFELAHIQPYLYPDYENVLQWRLSLRNNNFFEFMSGGGGGPAH
                     GILAVISRNEQKTQHPEYYAILDGVRMNGDNPVPDLCSQGLLQENVEYVKAMFDLYNG
                     SIVNVMPTDGFRGPSESSAECLAMEDDSRGFESALSEYTWEYVNNVAWEVYNDPKYSG
                     KKIINAAYPPYLFPPRNLSKPVAPNLVVMITRWRSDFASEQERQKYLDLKNQWLDILP
                     SKEIYTYDYYLHNRPDTFTQGIPSYFPHIIVEDLDDLKSNSKGEFMEVYSNWPLDNRE
                     WDTFAATSFNVYVTARAYWDTNLDVDALLEEYYTLYYGPASSQMKAFVEYSEDNYIRA
                     LEDPTVLRTMRSMLASAKSVAGDTIYGQRIDLLIGLMNSDFEGDEVNINSCRTLDSPT
                     TTYKLTSDVSSTGTCFNINHVGITLDCQGHTITSGDGRGIAELYWYTNGNALTIKNCN
                     IVSTGSTIGRGIEIHYANDVKIENSNITAANTGIWLVNNGNPNITNNRIISSSSTPLS
                     TQTVYGGYIHKNNLTSNSDWGMYLYDTHNMNVTFNNASSNSNRYGAVLMKSTQNTITD
                     NRFISNGGRGLWEYECSGNVLLRNEIILP"
     gene            complement(2667..3599)
                     /locus_tag="PLU55_03010"
     CDS             complement(2667..3599)
                     /locus_tag="PLU55_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87057.1"
                     /translation="MGKSKRAKLKKYLILSTILIILVLSIFFFLKESNSFSIFDNSSS
                     TKLFGKSSASQIPAETCSDGILNQDEEKVDCGGTICGPCPTCHDGIQNQGERHIDCGG
                     PCDACPPDCYDGILNQDEEKVDCGGTICGPCPTCNDGIQNQGEGGIDCGGPCPIACPP
                     TCHDRIQNQGEEGIDCGGPCSACVIAPVILTSLTPEEATKVKNAILSSEFISAMPNNG
                     VISIQFYYFPVGERIWLDTFLIGKNQILTSGNPDMNVIIHSKYISELPSSSLCDIIQK
                     ARTNGDFAYDTQLGDAKLLLKYSGMIKYRDCFGF"
     gene            3756..3839
                     /locus_tag="PLU55_03015"
     tRNA            3756..3839
                     /locus_tag="PLU55_03015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3790..3792,aa:Leu,seq:taa)
     gene            3848..4354
                     /locus_tag="PLU55_03020"
     CDS             3848..4354
                     /locus_tag="PLU55_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87058.1"
                     /translation="MILLIHIDKMQENANIPKIKKKVPLLIKILAGFNFICALFLFIS
                     FFSIIIDNQFEILVFIIAIIVTLGGTILFFFAGRGLLKAKNYARIMNIIFAILGFIIT
                     ICLILLVAKLNLIIQEANQNVDANITNLMSMFRVNVFLPKAIISLVVNFLIALYLLID
                     KQVKETFK"
     gene            4384..4905
                     /locus_tag="PLU55_03025"
     CDS             4384..4905
                     /locus_tag="PLU55_03025"
                     /inference="COORDINATES: protein motif:HMM:NF021425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HPJ87059.1"
                     /translation="MVKRKVTKKGVKKKSEPVKAKKKFEYHDDAPIIVKLLSIFNYVN
                     GGLWALIGFIIIFAAGGIVSYILQVSPELFVGYESGSLVTMLILAGIVMVLLAVLHFF
                     VGIGLWRLKPWARIVSIILSTIGVIGTIYSMIINFAPTQIFNIVVDGFIVGYLLFSKE
                     AKEAFKKNKKLIK"
     gene            complement(4894..5892)
                     /gene="prs"
                     /locus_tag="PLU55_03030"
     CDS             complement(4894..5892)
                     /gene="prs"
                     /locus_tag="PLU55_03030"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HPJ87060.1"
                     /translation="MKDVVLLADPNTAAWDFSEKIQNHIRDTKEKEVRLDRLEITVFG
                     DDEFLPHVVDNIRKKDVYLIQSSNKNPGTWLTELVLTKNLCLKADVNSLSLVLPYMRF
                     VRQDRKHQSRVPISTRAVADAISPGLKRIITMDLHSPQIENAYPENVALDNLQSFPDL
                     VRYLKENKSSYLDNLAIVSPDVGGAGRANSLYKRFLKTEKKSNGDESKYSLAIMSKFR
                     DKPGSIEKMFLIGDVNKKNVLVVDDIIDSGNSGIKAADLLRKEGAQKLMFYATHGLFT
                     KGTKDILNAYDVVMTSNTHYSSKEGDRKIEIIDMAPTFAEAIYRSQEGLSVSRLFD"
     gene            complement(5983..6606)
                     /locus_tag="PLU55_03035"
     CDS             complement(5983..6606)
                     /locus_tag="PLU55_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPJ87061.1"
                     /translation="MIKAIILDFGSVIYKTKWEKINEFFYQKNGFNILFGKSEDAELI
                     RIYSESDVGKEDFKKFFLRLKPDLQDVDAVVKDYKEGYSKFKEMNQELLDIIKELKEK
                     GIILFGFTDAKKEHYDANMEIGIYDGFEEIFTSFNFKCLKSDAKAFELLTKRLNEYGL
                     NPEECLFVDDNLENVKRAKEKGYKIIHYQNFPEVEEIKKELENIFVF"
     gene            6812..9568
                     /locus_tag="PLU55_03040"
     CDS             6812..9568
                     /locus_tag="PLU55_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87062.1"
                     /translation="MPAYSYHPCKEFSKNSQYYFSICRDAGHGGVCFDKYSGEYQGCA
                     PISGLYCTSGHANANKNIICSTEIPRIMYWDGKVNQHIDLNTGLWTTDPDGTSGSGID
                     KLTYCKKWYPNTVSVKFYKEETIRTWRGRGNRGYYESTKPSYECVQPNSVKCEESDGG
                     INYYLKGQVVAPIELGEGFGGWDYCTNGEGMPLSNTGKYLVEWYCTSNGGANNRLYLC
                     PNGCVNGACVQQTGKYCSVDATECGLEWTAHICGKVIYSADGSKELCEIPGVGPGLIS
                     AGFVLNSVLTDGYSCAKSVICPGKCEAGVCIKSTTDNLEELCLRNDIEVTKVINTNSR
                     DYQVTITRKAGKDNMGIMLVFTNAKQSSNYVVRWDLDGSGSHAPLMVNQNRIIDINAA
                     NNNQMPANLNPVEVSVTKYFKDESGNPKLCSTTSKFVFRGDPTIPSKEAESRCEETDG
                     GIDYSKQGVLGVSYRYNAIVKEGKRENLGGRIVSIEFIDAESVRLNIDGVISPSISKG
                     NSYLNSMDTVKIEDIQFSGKGTTLSSVNVTIINSSATIFKDFCINEAILKEFYCEGND
                     GPYNVTRDCGMEGKICLNGACVKEAESSCEDSDGGIDYSKQGVLGVSYRYNAIVKEGK
                     RENLGGRIVSIEFIDAESVRLNIDGVISPSISKGNSYLNSMDTVKIEDIQFSGKGTTL
                     SSVNVTIINSSATIFKDFCINEAILKEFYCEGNDGPYNVTRDCGMEGKICLNGACVKK
                     EELSCKDSDGGKNYQEKGTINFGGQIVFEDYCFNDVFSTTTELKEGVCYEDGSWGELY
                     YQCPDGCVNGACVKDVPECVYDSDCDYGYKCLNKACVKEVVGNICESGCLSNGKCYPM
                     GYRRSGTYCSEDNEFVKLLPSDSSCNNNFECRSNLCIGDECVDQSLINRFIEWLRNSF
                     GSKK"
     gene            9603..>10017
                     /locus_tag="PLU55_03045"
     CDS             9603..>10017
                     /locus_tag="PLU55_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPJ87063.1"
                     /translation="MKKAGYSVLLLVLILAFIVLNYNSFDSWVVKNLETLEPVEVERV
                     IDGDTVVINGESVRMLGLNTPERGEKYYKEAKEYTSSLVMNRTVLIERRGKDKYNREL
                     GYLFYGDENLNAKIVEKGYANYYFPSGHDNYYGLFE"
BASE COUNT         3430 a         1234 c         1819 g         3534 t
ORIGIN      
        1 gggggaagtt ctggtgatgg aagtagcgga ggaagtccaa gttgtaatga tacttgtgct
       61 tctttagtta aacaatgtgg aacacatata atttgcggag agagtgttaa ttgtggaaat
      121 tgttcaactg gatatgtttg tagtttaggt ttatgtattc caaatgtttg tactcctaat
      181 tgtgttggaa aaaattgtgg aagtgatgga tgtggaggaa gttgtggaac ttgttcaact
      241 ggatatactt gtcaaataaa tggaacatgt cttaaagatg atgttaattt aagatatatt
      301 gttaaaaatg gggtaggata tgcagatatt attgttgctt ctaatgctcc aaatattgtt
      361 aaacttgcag cagactattt ccaaatatat atttataata tgactggagt tagattacct
      421 atttcaaatt ctgttaacca aagtaaagat tatcatattt atattggaag aagtagttat
      481 acagacaact tgggtataaa tagccaagga tgtgagaatg atggattcaa aatggtttct
      541 ggagcaaact acttggcttt gattggagat gatgctccat ttgtcctttc aggtcctgat
      601 aattatccag aatgggattc cttaactgga aaaaaatggg caaatgatta tattgattat
      661 tatggtaaac aatataatgg actaaaagat atttggatga ctgatggtaa aggttctctt
      721 aatgctgtgt ttgaattttt atataatcaa ggaataagat ggtatgctcc aggagaaatt
      781 ggaacaatta ttcctgaaaa atcaagtatt ggttttggag atatgaatgt attaattaat
      841 cctgatgttc ctatgagaag tatgtttcat tattattttg aattagcaca tattcaaccg
      901 tatttatatc cagactatga aaatgtgttg caatggagat taagcttaag aaataataat
      961 ttttttgagt ttatgagtgg aggaggtgga ggaccagcac acggtatatt ggcagtaata
     1021 tctcgtaatg aacaaaaaac tcagcatcca gaatattatg caattcttga tggtgtaaga
     1081 atgaatggag ataatcctgt tcctgattta tgttctcagg gattattaca ggaaaatgta
     1141 gaatatgtta aagctatgtt tgatttatat aatggttcta tagtaaatgt catgccaaca
     1201 gatggattca gaggaccttc tgaatcaagt gctgaatgtt tggcaatgga agatgatagc
     1261 agagggtttg aatcagcatt gtcagaatac acttgggaat atgtaaataa tgttgcatgg
     1321 gaagtttaca atgatccaaa atattctgga aagaaaatta ttaatgcagc atatcctcct
     1381 tatctatttc ctcctagaaa tctttcaaaa cctgttgctc caaatttagt tgttatgata
     1441 acaagatgga gaagtgactt tgcttcagaa caagaaaggc aaaagtattt ggatttaaag
     1501 aaccagtggt tggatattct tccttcaaaa gaaatttaca cttatgatta ctatttacat
     1561 aataggcctg acactttcac tcaaggaatt ccaagttatt ttcctcatat aatcgttgaa
     1621 gatttagatg atcttaagag taattctaaa ggtgaattta tggaagttta tagcaattgg
     1681 ccattagata atcgagaatg ggatacattt gctgctacat cattcaatgt ttatgttact
     1741 gctcgtgcat attgggatac aaatctagat gttgatgcat tacttgaaga atattatact
     1801 ttatattatg gacctgcaag ttcacagatg aaggcttttg tagaatattc agaggataat
     1861 tatatccgtg cacttgaaga cccaacagtt ctaagaacaa tgagaagtat gcttgctagt
     1921 gccaaatctg ttgcaggaga tactatttat ggtcaaagaa ttgatttgtt aattggtttg
     1981 atgaattcag actttgaagg agatgaagtg aatattaact catgtagaac acttgattct
     2041 ccaacgacaa cttataagct tacttcagat gtttcttcaa caggtacttg ctttaatata
     2101 aatcatgttg gaataacatt ggattgtcag gggcatacta taacttctgg agatggaaga
     2161 ggtattgcag aactttattg gtatactaat ggaaatgcac ttacaattaa gaattgtaat
     2221 atagtaagta ctggttcaac gattggtagg ggaatagaga tacattatgc aaacgatgtt
     2281 aaaattgaaa attcaaatat aacagcagca aacactggaa tctggttggt taataatgga
     2341 aatccaaata ttaccaataa taggataatc tcttcttcaa gtacaccttt atctactcaa
     2401 acagtttatg gaggatatat ccacaagaat aatttaacaa gtaattctga ttggggaatg
     2461 tatttatatg atactcataa tatgaatgtt acattcaata atgcttcaag caattccaac
     2521 agatatggag cagtattaat gaaaagcaca caaaatacaa taactgataa cagatttatt
     2581 agtaacggtg gaagaggatt atgggaatat gaatgttctg gaaatgtttt attaaggaat
     2641 gagataattc ttccataaaa aatattttaa aatccaaaac agtctctgta tttaatcatc
     2701 ccagaatatt ttagaagaag tttagcatct cctaattgag tatcgtaagc aaaatctcca
     2761 tttgttcttg ctttttggat tatatcacat aaacttgaag aaggcaattc tgaaatatat
     2821 tttgaatgga ttattacatt catatctgga tttccacttg ttaatatttg attctttcca
     2881 attaaaaatg tgtctaacca gattctttct cctactggaa aataataaaa ttgaatagaa
     2941 attactccat tattaggcat agcagaaata aattcacttg ataaaattgc attttttact
     3001 ttagttgctt cttctggagt taaagaagtt aatataactg gagcaataac acaggctgaa
     3061 caagggccac cgcaatcaat tccttcttca ccttgatttt gtattctgtc atggcaggtt
     3121 ggaggacagg caatagggca aggaccacca caatcaattc caccttcacc ttgattttga
     3181 ataccatcat tacatgttgg acaaggtcca caaattgttc caccacaatc aactttttct
     3241 tcatcttgat ttaatatgcc gtcataacaa tcaggagggc aagcgtcaca tggacctcca
     3301 caatctatat gtctctctcc ttggttttgt attccatcat gacaagttgg acaaggtcca
     3361 caaattgttc caccacaatc aaccttttct tcatcttgat ttaatatacc atcagaacag
     3421 gtttctgctg gtatttgaga agcagaactt tttccaaaaa gctttgttga gcttgaatta
     3481 tcaaatattg aaaaagaatt gctttctttc agaaaaaaga atattgataa gactaatata
     3541 atcaaaatag tagataaaat caaatatttt ttcaatttag ctcttttaga ttttcccata
     3601 atgaatattt aaatcagtgc aagaagatta taaaggtttt gaatttattt aattcttgaa
     3661 aatttaatga ttagtaagat ttataattaa ttcttagcta ttttttctgt aatattgtac
     3721 taatgtgcct gatttgtcag gattttatat actaggcgag tgtgccggag tggtcaaacg
     3781 ggctggactt aagatccagt agctttatgc ttgcgaaggt tcgaaccctt tcactcgcat
     3841 ttaggagatg attctcctca ttcacattga taagatgcaa gaaaatgcta acattcccaa
     3901 aataaaaaag aaagttcctc tactaattaa aatacttgca ggatttaatt ttatttgtgc
     3961 tctattttta tttatcagct ttttttcaat tataattgat aatcaatttg aaattcttgt
     4021 atttataatt gcgataatag ttacacttgg tggaacaatc ttgttctttt ttgctggaag
     4081 gggtttattg aaagctaaaa attatgcaag aattatgaat ataatatttg ctattctcgg
     4141 gtttataata acaatctgtt taattttgct tgttgctaaa cttaacctca ttatacaaga
     4201 agcaaatcaa aatgttgatg caaatattac caatcttatg agtatgttca gagttaatgt
     4261 ttttttacct aaagcaatca tatcccttgt agttaatttt ttaattgcat tatatctttt
     4321 aatagacaaa caagtaaaag aaacatttaa gtagagaaaa taattataaa atcaagagga
     4381 gaaatggtaa aaaggaaagt aacaaaaaag ggtgtgaaaa agaaatctga acctgtaaaa
     4441 gctaaaaaaa agtttgagta tcatgatgat gctcctataa ttgttaaact actttcaatt
     4501 tttaattatg ttaatggagg gttatgggct ttaattggat ttattattat ctttgctgca
     4561 ggagggattg tttcttatat tttacaggta tctcctgaat tatttgtagg ttatgagagt
     4621 ggaagtttag tgactatgct catacttgct ggaatagtta tggttttact tgctgttttg
     4681 catttttttg tgggtatagg attatggaga ttaaaacctt gggcaagaat agtttcaata
     4741 atattaagta caataggagt tattggaaca atttattcta tgataatcaa ttttgcacct
     4801 actcaaattt ttaatatagt agttgatgga tttattgttg gttatttatt attttcaaaa
     4861 gaagctaaag aggcatttaa gaaaaataaa aaattaatca aataatctag agacagataa
     4921 accttcttgt gacctgtaaa ttgcttctgc aaatgttgga gccatatcaa ttatttctat
     4981 ttttctgtct ccttctttgg aggaataatg agtattgctg gtcattacaa catcataagc
     5041 gttgagtata tcttttgttc cttttgtaaa taaaccatga gttgcgtaaa acatgagttt
     5101 ttgagctcct tcttttctta acaaatcagc tgcttttatc ccagaatttc ctgagtctat
     5161 aatgtcgtct acaaccaaga catttttttt attaacatct ccgattaaaa acattttttc
     5221 aatgcttcct ggtttatctc tgaatttgct cattattgct agagaatatt tactttcatc
     5281 tccattagat tttttttctg tttttaaaaa acgtttatac aaagaatttg ctcttcctgc
     5341 tcctcctaca tctggtgaaa caattgctaa attatctaaa taagaagatt tgttttcttt
     5401 taagtatctt acaaggtctg ggaaactctg aagattatct aatgcaacat tttcaggata
     5461 agcattttct atttgtgggg agtgtaagtc cattgttatt attcgtttaa gtcctggtga
     5521 aattgcatct gcaactgctc ttgttgaaat tggaactctt gactggtgtt ttctgtcttg
     5581 ccttacaaat ctcatataag gtaaaaccaa acttaaactg ttaacatctg ctttaaggca
     5641 gagatttttt gttaaaacaa gttctgttaa ccatgttcca gggtttttat ttgaagattg
     5701 tattaaataa acatcttttt ttcttatatt atctacaaca tgagggagaa attcatcatc
     5761 tccaaagact gttatttcta atctgtctaa tcttacttct ttttcttttg tgtctcttat
     5821 gtggttttga attttttcag agaaatccca tgcagctgta ttagggtcag cgagtaaaac
     5881 cacgtctttc ataattgaaa aaaaagaaat tgatttataa taattgttat tgtaaaatat
     5941 atttgattta tgaaaactta aaagagaaaa agtttagata catcaaaaaa caaatatatt
     6001 ttctaattct ttttttattt cttctacttc tggaaaattt tgataatgaa taattttgta
     6061 acctttttct tttgctcttt taacattttc caagttatca tcaacaaaca aacattcctc
     6121 tggatttaaa ccatattcat taagcctttt agttaatagt tcaaatgctt ttgcatctga
     6181 ttttaaacat ttaaagttaa atgaggtaaa aatttcttca aaaccatcat aaattcctat
     6241 ttccatgtta gcatcataat gttctttttt agcatctgtg aatccaaaca atattattcc
     6301 tttttcctta agttctttaa ttatgtctaa taattcttga ttcatttcct taaattttga
     6361 ataaccttct ttataatcct taaccactgc atcaacatct tgcaaatcag gttttaatct
     6421 taagaaaaat tttttaaaat cttcttttcc aacatctgat tcagagtaaa ttcttatcaa
     6481 ttctgcatct tctgattttc caaataaaat attaaaacca tttttctgat agaaaaattc
     6541 atttattttt tcccattttg ttttatatat aacacttcca aaatcaagta ttattgcttt
     6601 aatcatggat taatatgcat caattttttt aaatattttt ctttagcttc ataagaagtt
     6661 tcaaggaaag atttataaat atgacttaat ttaatttttc atgaaaagag gtttcacagt
     6721 atttcttgtt ttattattaa cagttagctt ataatttgtt tctgctggat tattcagtaa
     6781 aaataaaata acaggtgaag ctacaattac tgtgcctgcc tattcttatc acccttgtaa
     6841 agaattttcc aaaaactccc agtattattt tagtatatgt agagatgctg gtcatggagg
     6901 agtttgtttt gataaatata gtggagaata tcaagggtgt gcgccaattt ctgggttata
     6961 ttgtacatct ggacatgcta atgcaaataa aaatataatt tgctcaactg aaattccaag
     7021 aattatgtat tgggatggaa aagtaaatca gcatattgat ttaaatacag gattatggac
     7081 tacagaccct gatggaacaa gtggttcagg tatagataaa ttaacttatt gtaaaaaatg
     7141 gtatccaaat actgtttctg ttaaatttta taaagaagaa acaataagga catggagagg
     7201 tagaggaaat agaggttatt atgaatctac aaaacctagt tatgagtgtg ttcaacctaa
     7261 ttcagtaaag tgtgaggaga gtgatggggg aattaattat tatcttaaag gacaagttgt
     7321 tgcgcctatt gaactagggg agggttttgg aggatgggat tattgtacta atggagaagg
     7381 aatgccatta tcaaatacag gaaaatattt agtagagtgg tattgtactt caaatggagg
     7441 agcaaacaat agattatatt tatgtcctaa tggatgtgtg aatggggctt gtgttcaaca
     7501 aacaggtaaa tattgttcag tagatgcaac agaatgtggt cttgagtgga cagctcatat
     7561 atgtggaaaa gtaatttatt ctgcagatgg aagtaaagaa ctttgtgaaa ttcctggagt
     7621 tggtcctgga ttaatatctg cgggatttgt tttaaatagt gttttaactg atggatatag
     7681 ttgtgctaaa agtgttatat gtccaggcaa atgtgaagca ggagtttgta taaagagtac
     7741 tactgataat ttagaagaac tgtgtttaag aaatgatatt gaagttacaa aagttattaa
     7801 tacaaattct agagattatc aagtaactat tactagaaaa gctgggaaag ataatatggg
     7861 aattatgtta gtatttacta atgctaaaca atcctcaaat tatgttgtta gatgggattt
     7921 agatggaagt ggttcacatg ctcctttaat ggtaaatcaa aatagaataa tagatataaa
     7981 tgcggcaaat aacaatcaaa tgcctgctaa tttaaatcct gttgaagttt ctgttacaaa
     8041 gtatttcaaa gatgaatcag gaaatcctaa actgtgtagt actacatcaa aattcgtttt
     8101 cagaggagat ccaactattc cttctaaaga agcagaatca agatgtgagg aaactgatgg
     8161 gggaattgat tattctaaac aaggagttct tggagtttca tatagatata atgctattgt
     8221 taaagaaggt aaaagagaaa atttaggtgg aagaattgtt tcaatagaat ttatagatgc
     8281 agaatcagtt agattaaata ttgacggagt aataagtcca tcaatatcta aaggaaattc
     8341 ttatttaaat agtatggata ctgtaaaaat tgaagatata caattcagtg gaaaaggaac
     8401 aacactaagt tcagttaacg ttacaattat aaatagctct gctacaatat tcaaagattt
     8461 ttgtattaat gaagctattt taaaggaatt ttattgtgaa ggaaatgatg gaccatataa
     8521 tgtaactcgt gattgtggta tggaaggaaa aatatgcttg aatggagcat gtgttaaaga
     8581 agcagagtca agctgtgaag atagtgatgg aggaattgat tattctaaac aaggagttct
     8641 tggagtttca tatagatata atgctattgt taaagaaggt aaaagagaaa atttaggtgg
     8701 aagaattgtt tcaatagaat ttatagatgc agaatcagtt agattaaata ttgacggagt
     8761 aataagtcca tcaatatcta aaggaaattc ttatttaaat agtatggata ctgtaaaaat
     8821 tgaagatata caattcagtg gaaaaggaac aacactaagt tcagttaacg ttacaattat
     8881 aaatagctct gctacaatat tcaaagattt ttgtattaat gaagctattt taaaggaatt
     8941 ttattgtgaa ggaaatgatg gaccatataa tgtaactcgt gattgtggta tggaaggaaa
     9001 aatatgcttg aatggagcat gtgttaaaaa agaagagtta agctgtaaag atagtgatgg
     9061 aggtaagaat tatcaggaaa agggtaccat taactttgga ggacagattg tttttgaaga
     9121 ttactgcttt aatgatgttt ttagtacaac tactgaatta aaagaaggtg tatgttatga
     9181 agatggttca tggggggaat tatattatca atgtccagat gggtgtgtta atggagcatg
     9241 tgttaaagat gttccagaat gtgtttatga ttctgattgt gattatggat ataaatgttt
     9301 aaataaagca tgtgttaaag aagttgtagg aaatatatgt gaatctggat gtttatctaa
     9361 tggaaaatgt tatcctatgg gatatagaag gtcaggaaca tattgttcag aagacaatga
     9421 atttgttaaa ttattgccaa gtgatagtag ttgtaataat aattttgaat gtagaagtaa
     9481 tctttgtatt ggtgatgagt gtgttgacca aagtttaatc aatagattta tagaatggct
     9541 tagaaattct tttggttcaa aaaaatagaa ataaatttat agtttatttt ttagattaaa
     9601 ttatgaaaaa agccgggtat agtgttcttt tgcttgtttt gattttagct tttatagttc
     9661 ttaattataa ttcttttgat agctgggttg ttaaaaatct tgaaactctg gaacctgttg
     9721 aggttgaaag agttatagat ggagatactg ttgtcataaa tggagagagt gtaaggatgc
     9781 ttgggcttaa tactcctgaa agaggtgaga aatattacaa agaagctaaa gaatatactt
     9841 cgagtttagt tatgaataga acagttttaa ttgaaagaag aggtaaggat aagtataata
     9901 gggagttagg atatttgttt tatggggatg agaatttgaa tgctaaaatt gttgaaaagg
     9961 ggtatgcaaa ctattatttt cctagtgggc acgataatta ttatggatta tttgagg
//
LOCUS       DAOPCH010000030         9666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_6725_length_9666_cov_4.950473, whole genome shotgun sequence.
ACCESSION   DAOPCH010000030 DAOPCH010000000
VERSION     DAOPCH010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9666
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6725_length_9666_cov_4.950473"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLU55_03050"
     CDS             complement(<1..471)
                     /locus_tag="PLU55_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPJ87064.1"
                     /translation="MKILAVGDPHGEINKIKNISLREIDLVLITGDIGKADFARKRFF
                     EDIKRQKEGLPKLELTKTDVKRIYSEIYDSTMGILKYISKTVPTYSILGNVGADMIKG
                     SEVKKDETKFKVKLPYLREGVSNLNNFYLVKNAVRNISGLRIGFLEYFIDTSWVK"
     gene            complement(473..1924)
                     /gene="gatE"
                     /locus_tag="PLU55_03055"
     CDS             complement(473..1924)
                     /gene="gatE"
                     /locus_tag="PLU55_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004590152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HPJ87065.1"
                     /translation="MSDIDYSKLKFKSGLEIHQQLDTHKLFCNCPSILRGDLPDFYVE
                     RKLHAVAGESGEVDRAAEYQASLKKTFVYQGYRNSTCLIELDEEPPHEINEDALKIAL
                     QIALLLNCKIIPVTQIMRKTVIDGSNTSGFQRTVLIARDGYVETDFGRVGIETICLEE
                     DAARKVKVEGEKVYWNLDRLGIPLVEIATSPDIKSAEQAKEVAMYIGDVLRSCKVKRG
                     IGTIRQDVNISILEGNRVEMKGVQDMRTFIKTIENEVLRQKELYDEGTPTQPEVRNAL
                     PDCSSEFLRHMPGADRMYPETDLPLLKLSRDFIDSLKKDLPKLRSESAGELRERGLSE
                     EMVKLILGEDKIEELKSLAEIYSNLNFIAKMIVLFPKEIASKENKTLEEVEEQVMDYY
                     GDILRLLNKKKIVEGDVKDILINLVKGMDFKEAIKIEKVDNSEIEEEILKIIKEKPGL
                     NANAYMGLVMAKFKGKINGKQAMDVINKLMNKE"
     gene            complement(1963..2559)
                     /locus_tag="PLU55_03060"
     CDS             complement(1963..2559)
                     /locus_tag="PLU55_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87066.1"
                     /translation="MAPLCNELFDISNLNSLVRAEFNKEINLKDYDTGFFRTPKNFKD
                     FSFIPKKGLNLGRIIDILEDDLTKKGADYFYLSQTIPGICEAIKNAYEHGNLEDNNKN
                     IFLLRNFSKGNIEYVVGDEGGTINGNFIPYIFLIRENKKENSLDSAKDFYSFCGKNYA
                     PKGHSGVGTKTMHKCFNQVKYFKNEQGGLMVNLIKPSF"
     gene            2673..3068
                     /locus_tag="PLU55_03065"
     CDS             2673..3068
                     /locus_tag="PLU55_03065"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HPJ87067.1"
                     /translation="MKKKEVIRKNVRSIFRPTNFGQKASDKITIWIGSWPFIILFVLL
                     LIIWIVAIILLSKDTLDIDHFLILNLFLSCVAAIQAPIILMSQNRSSQKDRKRMEYDY
                     QVDRRTEKEIKKIKIQLDRIESKLNQRKY"
     gene            3093..3278
                     /locus_tag="PLU55_03070"
     CDS             3093..3278
                     /locus_tag="PLU55_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87068.1"
                     /translation="MVLKNSIIEKLATEGKITYIPAKESKKIYDNLIKRGIEYKRKYK
                     RKEFYSHIAASELVLNS"
     gene            complement(3279..4613)
                     /gene="gatD"
                     /locus_tag="PLU55_03075"
     CDS             complement(3279..4613)
                     /gene="gatD"
                     /locus_tag="PLU55_03075"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003217.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HPJ87069.1"
                     /translation="MVKNLKKKVRVKSERFSGVSKIESAKPGDLVEVHFGKVIYEGTF
                     LESPDSEKGIILLKLSNGYNIGLNKKEIIKLKVLKEFKEKDVDKEIKKDPSKPNIAMI
                     ITGGTISSKYDSKTGGVHPLDSPESLFRFYPELFERVNVLKIETPFMKFSEEMNFKDW
                     QKLAKLCEKLLNDSNIRGVVITHGTDFLHYTSAALSFMLGKLNKPVVLTYSQRSIDRA
                     SSDANLNLICSSIIAGISDIAEVVLVGHANSEDNFCNVMPGTKVRKLHTSRRDAFKVV
                     NGKSIARVYPNGNLEILSSFNKRGDVKKAKCLKNFEEKVALIKFYPGQSPDILDFYYG
                     KKYKGIVLELSGIGNSSESWIKKIRTLSGKGMIFCGTSQCINGRVDGLVYSNGRKLID
                     AGVIYLGDMLSETALVKLGWVLGNKEWMKSKNLIKEKMLQNISGEFNKRLEE"
     gene            complement(4653..4777)
                     /locus_tag="PLU55_03080"
     tRNA            complement(join(4653..4687,4741..4777))
                     /locus_tag="PLU55_03080"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4742..4744),aa:Arg,seq:tcg)
     gene            4876..6357
                     /gene="proS"
                     /locus_tag="PLU55_03085"
     CDS             4876..6357
                     /gene="proS"
                     /locus_tag="PLU55_03085"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPJ87070.1"
                     /translation="MTEKKIKEKTQEKSQEGITVKKEENFSEWFTQIIEKAELVDLRL
                     DVKGFTIIRPWAALTIENMFEHLEKELQKKGHKPTFMPSIIPKENLMKESEHVQGFTP
                     EVFWIKNTGDNKELALRPTSETLYTPMFKLWIRSHRDLPLKLYQRGSVFRMDTKATRP
                     LIRGREFIWIECHDAFATREEAEAQVQEDIDTTEKVMHQIYGVPFLPMKRPEWDKFAG
                     AEYTVGSDCFMPDGKLIQQPSTHLMGQKFSKAFDATFKNKEEKEEYLWTTAYGPAVSR
                     ILASVIATHGDDSGMILPYSISPIQVIIIPIFNAENKNKIIKKAKEISDKLEKENINV
                     EIDEREYKRPGEKYHIWELKGVPFRIEIGEKEIKNNEVILFTRDIKKKEKIKLTELKN
                     IQKLGKEFDNRLRKKADKFMENRLVSCSNKKEIKEALSSGKLAKINFCSTDKSSIKCA
                     EIVEKELYGDVRGTLANKNEKPTGNCVICNKPATKVVYIGRSY"
     gene            complement(6760..>9666)
                     /locus_tag="PLU55_03090"
     CDS             complement(6760..>9666)
                     /locus_tag="PLU55_03090"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPJ87071.1"
                     /translation="TIFNNYINIQSSHPIDVRHSSIYINITKNTVISNSGPVVYLAGS
                     KNSIIENNTVIANGSAGLYLLSSSDNCTLINNNVSNIGEGGDGVYIVNSNDVFVVNNT
                     VTGKYARAVNLYRSLNTVVLNQVAYGGDDGLEIWGSNNSIIKDCINLSGNVDDVFISS
                     SYGLSVNNTFINCSYDINKETVNGEENELIRKWYYQVYTNYSNGTAVAGVNISAYNTT
                     GDIQFTTLTNPSGLINRQEVIEYVNTGGTKNYYNNYTINATLFGHETESNIFNFTTTQ
                     NKVNDVFTFNPLVPGENACGVLDSPNTVYTLTRNVLSQGTCFTIAADNITLDCNAAWI
                     NYSINGENGTYGIYSDRFNTTIKSCKVIDMNHTSPNNERYGVYFSGASNGNIEYVYVN
                     TTNSQAIFVSSNSTNINLINNDAESLNHGIELRDSSKNNLINNTGIGLMSFSAGIVIN
                     GSNENTLINNTGIGNYSGILLGDKSSNNNILINNTATSNFSTALLISYSNNNYFISQN
                     ATSYNLGGDSVAIRISNSNNSLIKDCLSISGEDYDVYIGDNNVNNTFLNCSYDINKEY
                     LGIRGNLIRKWYYQTYVNYTNGTAVAGANITAYNKAGQIQFTHLTDSLGYIPLQELTD
                     YIGYNGIRNYYSNYSINTSKWLYNMESNVFNFTTKGNFVEDFLTLELDTFPPSYGYVG
                     HSSTTAGQKSKFSIKFTDRGALHPYGVYIFSTNNTGTWENDSAVNFTSTPSWANVSVT
                     LNSTVGDVVGYRWYFSDSFGNMNMTPIYKLVTEEKEESGGEGTGGGGGKKETPSQENE
                     DSLYRPSYEVLQRGYETYFRKGDIVQINLSKNGTYLFEIGDINRFTKNLTILFNSGKY
                     PVGLNESLKFDLNNDSFYDLQISVRDITASNSSRINFKEINEEIPASNKPALNQTSLG
                     NKSTINIFGINGEEKESKSVVYFVFVGIIVLALILVACAIFFRFRKAKETSVNL"
BASE COUNT         3517 a         1677 c         1106 g         3366 t
ORIGIN      
        1 tttaacccaa cttgtatcta taaaatactc taaaaaacct attcttaaac cagaaatatt
       61 tctaacagca tttttaacta aataaaaatt attaagatta ctaacacctt ccctaagata
      121 aggtaattta actttaaatt tagtttcatc ttttttaacc tctgaacctt ttatcatatc
      181 tgctccaaca tttcccaaaa tagaataagt tggaactgtt tttgaaatat attttaaaat
      241 acccatagta gaatcataaa tctccgaata aattctctta acatctgttt tagttaattc
      301 taattttggt agtccttctt tttgcctttt aatatcctca aaaaatcttt ttctagcaaa
      361 atcagcttta cctatatctc ctgttattaa aaccaaatca atttctctca aagaaatatt
      421 cttaattttg tttatctccc catgtgggtc tcccacagct aaaatcttca tcttattcct
      481 tattcatcaa tttatttatt acatccattg cttgtttacc attaatcttt cctttaaact
      541 tcgccataac caaacccata tatgcattag catttaatcc tggtttttct tttattatct
      601 taagaatttc ttcttcaatt tcagaattat ctactttttc tatctttata gcttctttaa
      661 agtccattcc tttaacaaga ttaattagaa tatctttaac atctccttca actatcttct
      721 ttttatttaa taaccttaaa atatcaccat aataatccat gacttgttct tcaacttctt
      781 caagagtttt gttttcttta gaagctattt cttttgggaa taaaacaatc atcttggcaa
      841 taaaattaag attagaataa atttctgcaa gagatttcaa ttcttctatc ttgtcctcac
      901 ctaaaattaa tttaaccatc tcttcactca atcctctttc tctaagttca cctgcactct
      961 cacttctcaa tttaggtaaa tccttcttta aactatcaat aaaatctcta gatagtttaa
     1021 gcaaaggtaa atcagtttca ggatacattc tatctgcacc aggcatatgt cttaaaaatt
     1081 ctgaactaca gtcaggtaaa gcattcctaa cttctggttg agttggagtt ccctcatcat
     1141 aaagttcttt ttgtcttaaa acctcgtttt caattgtttt aataaatgtt ctcatatcct
     1201 gaacaccctt catttcaact ctattgcctt ctaaaataga aatattaaca tcctgcctta
     1261 tagtcccaat ccctctttta actttgcaac ttctcaaaac atctccaata tacattgcaa
     1321 cttctttagc ttgttcagca gatttaatgt ctggagatgt tgcaatttca actaacggta
     1381 tccctaatct atctaaattc caatacactt tctctccttc caccttcacc tttcttgcag
     1441 catcttcttc caaacaaatt gtttcaatac ccactctccc aaaatcagtt tcaacatatc
     1501 catctcttgc aattaaaaca gttctctgaa atccagaagt attactccca tctatcactg
     1561 tctttctcat aatctgtgtt acaggaatta ttttacaatt aagaagcaac gcaatctgaa
     1621 gtgctatctt taaagcatct tcatttattt catggggagg ctcttcatct aattcaatta
     1681 aacaagtact atttctatac ccctgataaa caaaagtctt cttcaaagaa gcctgatatt
     1741 ctgcagccct atccacttct cctgattctc ctgcaactgc atgtaatttt ctctcaacat
     1801 aaaaatcagg taaatctcct cttaatatag aaggacaatt acaaaataat ttatgcgtat
     1861 ctaattgttg atgtatttcc aaacctgatt taaatttcag ttttgaataa tcgatatctg
     1921 acataaaaaa attaaacaaa gagggtttat taaaattttg aattaaaaag aaggcttaat
     1981 tagatttacc attaaaccgc cctgctcatt tttaaagtat ttaacctggt taaaacattt
     2041 gtgcattgtt tttgtaccaa ctccactatg tccttttgga gcataatttt ttccacaaaa
     2101 ggaataaaaa tcctttgcag aatctaaaga attttctttt ttattttcac gtatcaaaaa
     2161 tatgtaagga ataaaattac cattaatagt cccaccttca tctcccacaa catattctat
     2221 atttcctttt gaaaaatttc ttaataaaaa aatattttta ttattatcct ctaaattacc
     2281 gtgctcatat gcattcttaa ttgcctcaca aatacctgga attgtttggc ttaaataaaa
     2341 ataatctgct ccttttttag ttaaatcatc ttccaaaata tcaattatcc tgcctagatt
     2401 aagacccttc ttagggataa atgaaaaatc tttaaaattc tttggtgttc taaaaaatcc
     2461 tgtatcataa tcttttaaat taatctcttt attaaattca gctctaacta aagagtttaa
     2521 gttactaata tcaaataatt cgttacataa tggggccatt ttaagtaaaa tattaaggct
     2581 tattttactt ataaaccttt ctatatgtta ctatgtgtta gttacaacta attaataatt
     2641 aaacttataa ttagatttaa actaattaaa taatgaaaaa gaaagaagtg ataagaaaga
     2701 atgtgcgttc aatatttcgt ccaactaatt ttggacaaaa agcatcagat aaaataacta
     2761 tttggatagg tagttggcca ttcattattt tatttgtatt gcttttaata atctggattg
     2821 ttgcaattat tctattatca aaagatacat tagatataga ccattttctt atacttaatt
     2881 tatttttatc ttgtgtagca gccatacaag caccaataat attaatgtct caaaacagat
     2941 caagtcaaaa agacagaaaa agaatggaat atgattacca agtagataga agaactgaaa
     3001 aagaaattaa aaaaataaaa atacagcttg ataggataga aagtaaacta aatcaaagaa
     3061 aatattaaat acttaataaa attaaatata agatggtatt aaaaaacagt ataatagaaa
     3121 aattagctac tgaaggaaaa ataacttata ttcccgctaa agaatcaaaa aaaatctatg
     3181 acaacctaat taaaagaggt atagaatata agagaaaata taaaagaaaa gagttttatt
     3241 cacatattgc agcaagtgaa ttagttctta attcctaatt actcttccaa tcttttatta
     3301 aactcccctg aaatattctg aagcattttt tcttttatta aatttttaga tttcatccat
     3361 tccttatttc ccaaaaccca tccaagctta actaatgctg tttctgaaag catatctcct
     3421 aaatagataa ctcctgcatc aataagtttt cttccatttg aataaaccaa tccatccact
     3481 cttccattaa tacactgaga agttccacaa aaaatcatgc ctttccctga aagagttctt
     3541 atctttttta tccaagactc tgaagaattt ccaattccac tcaattctaa aacaattccc
     3601 ttatacttct ttccatagta aaaatcaagt atatcaggag attggccagg gtagaattta
     3661 atcaatgcta ctttttcttc aaaattctta agacatttag cttttttcac atctcctctt
     3721 ttattaaatg aacttaatat ttccaagttt ccattaggat aaactcttgc aatacttttt
     3781 ccattaacca ccttaaacgc atcccttctt gaagtatgca atttccttac ttttgtacca
     3841 ggcattacat tgcaaaaatt atcttctgaa ttagcatgtc caactaaaac aacttcagca
     3901 atatcactaa ttccagcaat tatagaagaa caaatcaaat taagattcgc atcagaactt
     3961 gctctgtcaa tacttctctg agaataagtt aaaacaacag gcttatttaa ttttccaagc
     4021 ataaaactaa gtgcagcaga agtataatgt aaaaaatctg ttccatgtgt tataactact
     4081 ccacgaatat ttgaatcatt taataatttt tcacataatt ttgctaattt ttgccaatct
     4141 ttaaaattca tttcttcaga aaatttcata aatggagttt ctatctttaa aacattaact
     4201 ctctcaaata attctggata aaatctaaac aaactttcag gactatctaa aggatgaact
     4261 cctcctgttt ttgaatcata tttagaagaa attgttcctc ctgttataat catagcaata
     4321 tttggttttg aagggtcttt tttaatttct ttatctacat ctttctcttt aaactctttc
     4381 aaaaccttta acttaattat ctcctttttg tttaatccaa tattatatcc attactcaat
     4441 ttaagtaaga ttattccttt ctcagaatca ggactttcaa gaaaagttcc ttcatatata
     4501 acctttccaa aatgaacctc aactaaatct cctggtttag ctgactcaat ctttgaaact
     4561 ccagaaaaac gctctgattt tactcttact tttttcttta aattttttac catattaaat
     4621 aaaaagaaat actaattaaa aacttaatga tatgagccca ggaggaattg aacctccatc
     4681 atcggggtat tccgaagccc gctcgcgata ataaataatt tagtttcgca aacttcttca
     4741 ccgaaacccg aaattctatc cgttgaacta tgggccctat atattaaaca ggtctaaaac
     4801 ctttttatac tttaatattt aaacataaac aatcaaatca aataatttta aatatgctct
     4861 ttagttcata taaacatgac agaaaagaaa atcaaagaaa aaactcaaga aaaatcacaa
     4921 gaaggaatta cagttaaaaa agaggaaaat ttctctgaat ggttcacaca aattattgaa
     4981 aaagcagaac tagttgattt aagattagat gttaaaggat tcacaataat ccgcccatgg
     5041 gcagctttaa caatagaaaa catgtttgaa cacttggaaa aagaattaca aaaaaaagga
     5101 cataaaccaa cattcatgcc ttcaataata cctaaagaaa acctaatgaa agaatcagaa
     5161 catgttcagg ggtttacacc agaggtattt tggattaaaa acacaggaga caacaaagaa
     5221 cttgcattaa gaccaacttc tgaaactctt tatactccta tgtttaaact ctggataaga
     5281 agtcatagag atttaccttt aaagctatac caaagaggct cagtttttag aatggacact
     5341 aaagccacgc gtccattaat acgaggaaga gaatttattt ggattgaatg tcatgatgcc
     5401 tttgcaacaa gagaagaagc agaagctcaa gtacaagaag acatagatac aacagaaaaa
     5461 gtaatgcatc agatttatgg ggtgcctttc ttacctatga aaagaccaga atgggataaa
     5521 tttgcaggag cagaatacac tgtaggctca gattgtttta tgccagatgg aaaattaatt
     5581 cagcaacctt caacacattt aatgggacaa aaattttcaa aagcatttga tgcaacattc
     5641 aaaaacaaag aagaaaaaga agaatatctc tggacaactg cctatggccc tgcagtctca
     5701 agaatattgg cctcagttat tgctactcat ggagatgatt ctggaatgat tttgccttac
     5761 tctatctccc caatacaagt aataataatt ccaatattta atgcagaaaa taaaaataaa
     5821 ataataaaaa aagcaaaaga aatatctgat aaattagaaa aagaaaatat aaatgtagag
     5881 atagatgaaa gagaatacaa aagaccagga gaaaaatatc atatttggga actaaaagga
     5941 gttccattca gaatagaaat aggtgaaaaa gaaataaaga acaatgaagt aatattattt
     6001 acaagagaca taaagaaaaa agagaaaata aagttaactg aattgaaaaa catccaaaaa
     6061 ctaggaaaag agtttgacaa tagattaagg aaaaaagcag acaagtttat ggaaaataga
     6121 ttagtttcgt gttccaataa aaaagaaata aaagaagctc tctcttctgg aaaattagct
     6181 aaaataaact tttgttctac agacaaatca agtataaaat gtgctgaaat agttgagaaa
     6241 gagctttatg gtgatgttag aggaacacta gctaacaaaa atgaaaaacc aacaggaaat
     6301 tgtgtaatat gtaacaaacc tgcaacaaag gttgtatata taggaagaag ctattaacta
     6361 atatttacaa tagacaaaac aattcaaata aaacatccct tttaattttc taattttatg
     6421 cagttgttaa gttcatcttc aagctgtata gttaaagcta ttaatttact caactcttgc
     6481 tcaactttct ttttttccat gtttgttttt ttgcttttca ttttatataa agcaaacatc
     6541 tcattaattc aagtttttca aatctagaaa gaaattgcct caatccaatc cagattttag
     6601 aactgaatta aattgagttc ggtttaatat tgaaagtata aactaaacac gaacatctat
     6661 cccacataga gacattcagt atgttcagtt tatcagtcat attaaaagta acaataaata
     6721 ctttataaat atttatataa acaatcaggc tttcgggcac tacaaattta ctgaagtttc
     6781 tttagctttt ctaaatctaa aaaagatagc acatgcaacc aatatcaatg ccaaaactat
     6841 aattcctaca aatacaaaat aaaccacaga tttactttct ttttcctctc catttattcc
     6901 aaaaatattt atagttgatt tattgcccaa agaagtttga ttcaacgcag gtttatttga
     6961 agcaggaatt tcttcattta tttctttgaa attaattctt gaactattgc ttgctgtaat
     7021 atccctaact gaaatttgta aatcataaaa gctatcatta tttaaatcaa atttaagaga
     7081 ttcgttcaat ccaacaggat acttacctga attaaacaaa atagtaagat tttttgtaaa
     7141 tctattaata tccccaatct caaatagata tgttccattt ttagataaat ttatttgaac
     7201 aatatcccct tttctgaaat atgtttcata acctctttga agaacctcat aagaaggtct
     7261 gtacaaagaa tcttcatttt cttggcttgg agtttctttc ttacctcctc cacctcctgt
     7321 tccttctccc ccactttcct ccttttcctc tgtaactaat ttataaattg gagtcatatt
     7381 catatttcca aaagaatcac tgaaatacca cctatatcca acaacatctc caacagttga
     7441 atttaatgtt acagatacat ttgcccaaga aggtgttgaa gtaaagttaa ctgcactatc
     7501 gttttcccaa gtaccagtat tatttgttga aaatatataa actccataag ggtgaagagc
     7561 tcctctatca gtaaatttaa tcgaaaattt agatttctgt cctgcagtag ttgagctatg
     7621 gccaacataa ccataactag gagggaatgt atctaattcc aatgttaaaa aatcttcaac
     7681 aaaatttcct ttggttgtaa aattaaaaac attactttcc atattataca accatttact
     7741 tgtattaatt gaataattgc tataataatt tcttatacca ttataaccaa tataatctgt
     7801 taattcttgt aatggaatat atcctaaaga atcagttaaa tgtgtaaatt ggatttgtcc
     7861 tgctttattg tatgcagtaa tatttgctcc tgcaactgca gtcccattag tataatttac
     7921 ataggtttga taataccact tacgtattaa attacctcta atacctaaat attctttatt
     7981 tatgtcataa gaacaattca aaaaagtatt attaacatta ttatctccaa tatacacatc
     8041 ataatcttct cctgaaatac tcaaacaatc cttaatcaaa gaattattag aattagaaat
     8101 tcttatagca acagaatctc caccaagatt ataagaagtt gcattctgac taataaaata
     8161 attattattt gaataagaaa ttaaaagcgc agttgagaaa ttacttgttg ctgtattatt
     8221 tataagaata ttattattag aagatttatc tcctaataga ataccagaat aatttccaat
     8281 acctgtatta ttaataagag tgttctcatt tgaaccatta ataacaattc ctgcagaaaa
     8341 actcatcaaa ccaattccag tattattaat caaattattt ttagaagaat cccttaattc
     8401 tattccatga ttcaagcttt cagcatcatt attgattaaa ttaatatttg ttgaattaga
     8461 agaaacaaaa attgcctgtg aattagtcgt attaacatag acatactcta tatttccatt
     8521 agatgctcca gagaaataaa caccatatct ttcattattt gggctagtat gattcatatc
     8581 tataacttta caacttttta ttgtcgtatt aaatctatca gaataaattc catatgttcc
     8641 attttctcca ttaattgaat aatttatcca cgctgcatta caatctaaag taatattatc
     8701 tgctgcaata gtaaaacaag ttccctgaga taaaacattt cgtgttaaag tataaactgt
     8761 atttggagaa tctaatactc cacaagcatt ttctcctgga actaatggat tgaatgtaaa
     8821 tacatcatta accttatttt gagttgtggt gaagttgaaa atattactct ctgtttcatg
     8881 cccaaataaa gttgcattta ttgtataatt attgtaataa ttttttgttc ctccagtatt
     8941 aacatattct ataacttctt gtctattaat taatccactt gggttagtca aagtggtgaa
     9001 ttgaatatct ccagttgtat tgtaagctga aatgtttact cctgcaactg cagttccatt
     9061 agaataatta gtgtatactt gatagtacca ctttcttatt aattcatttt cttctccatt
     9121 aacagtctct ttatttatat cataagaaca atttatgaat gtattattca cacttaagcc
     9181 atatgaagaa gagataaata cgtcatcaac atttccactt aaattaatac agtcctttat
     9241 tatagaatta tttgaacccc atatctctaa accatcatct cctccataag caacttgatt
     9301 caatacaact gtatttaaag aacgatacaa attaactgca cgagcatatt tccctgttac
     9361 tgtattgttt actacaaaaa catcgttgct atttactata tatactccat cacctccttc
     9421 tcctatatta gatacattat tattgattag agtacaatta tcagatgaag aaagtaaata
     9481 taatcctgca gaaccatttg caatgactgt attattctct attatgctat tttttgaacc
     9541 tgcgagatag acaactggtc cagaattact tattacagta ttttttgtta tatttatata
     9601 aatagaagaa tgacgaacat caattggatg agaactttgt atatttatat agttgttaaa
     9661 aatagt
//
LOCUS       DAOPCH010000031         9504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_6845_length_9504_cov_4.301725, whole genome shotgun sequence.
ACCESSION   DAOPCH010000031 DAOPCH010000000
VERSION     DAOPCH010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6845_length_9504_cov_4.301725"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLU55_03095"
     CDS             complement(<1..208)
                     /locus_tag="PLU55_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPJ87072.1"
                     /translation="MNKKILAFVCDGKKFLLLRNNPKDPSHGGDYWFTVTGSVEADES
                     IENAVRREVKEETNLNVSEILGLKW"
     gene            387..950
                     /locus_tag="PLU55_03100"
     CDS             387..950
                     /locus_tag="PLU55_03100"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HPJ87073.1"
                     /translation="MGAIFSYVVLPLLIFFARIADVSLGTIRIIFISKGFKVLAFLVG
                     FFEVLIWLLAINQIWSDFSNPWLLLAYAAGFATGNYVGIYLDEKISIGNSMIRIIVIK
                     NSKKLVNELKKNGYQLTVIDGHSADQNIDVKMILVVVKRKELKNVLKILKKINPNSFF
                     TIEDIRNVRKNRNELLKEKRSPRRLFK"
     gene            994..1506
                     /locus_tag="PLU55_03105"
     CDS             994..1506
                     /locus_tag="PLU55_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HPJ87074.1"
                     /translation="MKKNSLLYIVRNSKVHGKGVFARKDIPKGTKIIEYVGNIVSSKE
                     GLRIYEEQLKKSKETGVGAVYIFQLNRKQDIDGDVPWNPAKYINHSCNPNCKYKIINN
                     HIWIFSIRDIKKGEELNYDYDYDLDNYQDHLCKCNSKDCLGFIIGKRYRKKFKKLMKI
                     NLNIYKVYIH"
     gene            1513..1725
                     /locus_tag="PLU55_03110"
     CDS             1513..1725
                     /locus_tag="PLU55_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87075.1"
                     /translation="MKTSTKIIIAVVVIVVAVLIWGLVGSSEAAKIGTTCDFGIGEDG
                     SVLCWKWHRNAWGQTGDAINSWLEGK"
     gene            1760..2512
                     /locus_tag="PLU55_03115"
     CDS             1760..2512
                     /locus_tag="PLU55_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPJ87076.1"
                     /translation="MLNEIILALIQSLTEFLPISSDGHLALVSNIIGTPNIFFITFLH
                     LASLFAVLIFTRKEISEILKFNKESWRLIKYLIIGIIPAGLVGLFLSDLIESTLTSYF
                     LIGIFFFFTGIIVFSTKFIKPSKNKLSNKSAFSIGLMQALALLPGVSRSGMTISTAMF
                     FGIEKEKAVKFSFLMFIPLTLAAFIFETIKYSAEVMTIPALTLIVSFTVCFLFSLLFL
                     NLLNYIVKKDKFWLFGIYCFLIGLVSLIIYFI"
     gene            complement(2519..3844)
                     /locus_tag="PLU55_03120"
     CDS             complement(2519..3844)
                     /locus_tag="PLU55_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HPJ87077.1"
                     /translation="MQKTANKKTVSVLVIILIVIGVATAVTVGLFLFPYNPDEPPRAD
                     DVGSTPKGIQEVVNANNQFAFDLYSELAKTEKDNIFYSPYSISTALAMTYEGAKGKTA
                     EEIKEVFHFPETSVLRPNYAAIYNKINENNKEYQLSTGNALWVQQDYLLLNDYSEAVE
                     RYYGGKASNVDFIKETEKSRQTINSFIEEQTNNKVKELIPAGQVDYLTRLVITNAIYF
                     KGTWEWEFDKSDTRDKNFYITPENVVKTPMMYMDSDKATFNYADLEKLQILELPYKGD
                     KISMLILLPKQGEAYDYEKGEKITYNYNLEDIELSSEKLNEYKSEMQETKLGAIYLPK
                     FKFDTKYFMVSTLSEMGMPTAFSMQADLSGIDGSQNLYIQNVIHQAFVQVDEKGTEAA
                     AATSIMIGFKSAMPSNVFRADHPFIFIIQEKETGNILFMGRMTDPTKAQ"
     gene            3948..4259
                     /locus_tag="PLU55_03125"
     CDS             3948..4259
                     /locus_tag="PLU55_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87078.1"
                     /translation="MVKKKPSKKFVKAKIKPSHKSKKPNPKIFESYDDKKRAEWNKER
                     GKFEEIAEELSRDSDFIGLYNNILREKWVKQNYPRQKDTKALVRRAMDLYRIRIKKFS
                     I"
     gene            complement(4290..6005)
                     /gene="metG"
                     /locus_tag="PLU55_03130"
     CDS             complement(4290..6005)
                     /gene="metG"
                     /locus_tag="PLU55_03130"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPJ87079.1"
                     /translation="MVKNIKPKKKNRRLITSALPYINNVPHLGHIVGSHLPGDIFARY
                     SRARGYETLYIGGTDENGSTSEIAAFKLNVPIEKFADKLYKEHKKIYDWFEISYDSFS
                     RTSKKIHHETVKEFFETINKKGFIEKNKMKVFYSPTENMFLPDRYVVGECPKCGYPDA
                     SGDQCEKCTSVLDPSQLKNPKSTISGGKLEIRETEHLFLKLDSLTPKLKKWIQSNKYF
                     REQVRNLAMGWINEGLKPRCITRDLKHGVKVPLKEFKEKVFYVWFDAPIGYVSSTKEI
                     TKDWEKFWKDEKSEVYNFLGKDNIPFHTIFWPGMVIANGEFNLPKNVVGLQYLNYEGQ
                     KFSKSKGIGVFCERLPELKLEADVWRHYLTQIIPETSDSEFRWKDFQERVNSDLIGNY
                     ANYVNRVLNFIYTKLDRKITKPASKELQEIDKNLLKKIEEKRKSIGDLMERAELRKAY
                     AEILELSSEGNKYINDSEPWNVIKENPKRANNIFYISAFLLKNLAILFAPYLPKTSEK
                     VWKQLNLKGSPSCSGAWEEAEKDFGKEHEINKPELIFKKIEDKEIEEYRKITSQGIDL
                     TELFK"
     gene            6100..6861
                     /locus_tag="PLU55_03135"
     CDS             6100..6861
                     /locus_tag="PLU55_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87080.1"
                     /translation="MEEVFNTEEESNAFNRSQESEVFSTSKGHQVVSTERLEPKIVER
                     RIWNLNHIFLGLFIAIVFIAIGFFLIINYDLDLTNSLIVVLVIVVVYSSILFFLIEPK
                     VLRQVDNTVVQRVERPVLIEKPVVKEVPVEKKIFVDRPVEVIKEVPVEKEIIKEIEVP
                     IEKTVYIDRPVEKVVEVPSEPTIITVPQETKKRTPSLKYSFVASEETRVYHTKNCRLG
                     KLIKRKYKLVSDDEKDFIKAKFRPCKVCIKKTKKV"
     gene            6916..9483
                     /locus_tag="PLU55_03140"
     CDS             6916..9483
                     /locus_tag="PLU55_03140"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011320775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPJ87081.1"
                     /translation="MQSITESLQSMNLTGLTEREASSRLQKNGYNELPREKRKTTFKI
                     VLEVLKEPMFLLLIFCWIIYLILGDIKESFLLLGFVVVIIFITIYQENKTEKALDALR
                     NLSSPRALVIRGGQRKRIPGREVVQGDIIVLSEGDRVPADAIVVTSTNFFVDESILTG
                     ESVAAKKKPTKNFKILMQKPGGENTPFIYSGTLVVRGTAIAIVKSTGIETEMGKIGKS
                     LEVIKTEKTNLQKETNSLVKYFAIYGISACVLVIIAYGILKGNWIEAMLSGVTLAMSV
                     LPEEFPVVLTLFLGLGAWRMSKKNVLIRRQQAISELGSTTVLCTDKTGTLTQNKMAVT
                     KLYWNNQTCFIENNKKIPEQFHEVVEYGILASQKEPFDPMEKALKELSRGNESKFIHK
                     TWHLLQEYPLSDKLMAISHVWSKRGKSENIVAAKGAPEAIIELCRMDFNNKKRILKEV
                     ASFSKEGLRVIAVAKSSSIGPLPKDQHEFDFEFVGLIGFEDPIRPTVPQAIKECYDAG
                     IRVIMITGDYHGTAQNIALEIGLKNPDKYITGDELENLSDSQLKKKIKDVNIFVRVVP
                     QQKLRLVNALKSNGEIVAMTGDGVNDAPALKSANIGVSMGERGTDVAREASSIVLLDD
                     NFTSIVNGVKMGRRVFDNIRKAMSYVFSMHIPILGITVLALIFGWPLILLPVHILFME
                     LIIDPTCSIVFEAEKAEENIMKRKPRNPKEKIFNKRTLNHSIFQGLGMLLSMAALFYF
                     TFKSTNNEYLARAMAFSNLIFSSLLLILSSRSISKPFYKTILEKNKAFWFVAIGALFF
                     LALSLYTPFFNEVFRFSTLTIKELGISFGVALLGMLWFEIVKHTHYLHYDKKRFIPK"
BASE COUNT         3325 a         1364 c         1433 g         3382 t
ORIGIN      
        1 cccattttaa accaagaatt tcagatacat taagattagt ttcttctttt acttctcttc
       61 taactgcatt ttcaattgat tcatctgctt caacacttcc agttacagta aaccagtaat
      121 ctccaccatg agaaggatct ttagggttat ttcttaataa aagaaatttt tttccgtcgc
      181 atacaaaagc aagtattttt ttgttcatat tgagttaaga agatatttat ttaaaaatct
      241 tgtttgaact cactctctag atagtttaat tctggcaaat tgttaatctt tctcacactc
      301 catacaacat ttcttgggaa tttatctctt tctcacttta acacttcaag ataaccttta
      361 tatatcttaa acaataaatc tttatcatgg gggcgatatt ttcatatgta gtacttcctt
      421 tactgatatt ctttgcaagg attgcagatg tcagtttagg aacaataaga attatattta
      481 tctccaaagg tttcaaagta cttgcttttc tagtaggttt ttttgaagtt ctaatatggt
      541 tattggcaat aaatcaaata tggtctgatt tttcaaatcc ctggttatta ttagcatatg
      601 cagctggttt tgcaacagga aattatgtag gtatatattt agatgaaaag atttcaatag
      661 gaaattcaat gattagaata attgtaataa agaattctaa aaaattagta aatgaattaa
      721 agaaaaatgg atatcagtta acagtaatag atggacacag tgcagatcaa aacattgacg
      781 ttaaaatgat tttagttgtt gtcaaaagaa aagaactgaa aaatgtctta aagatattaa
      841 agaaaattaa tccaaactca ttttttacaa tagaggatat aagaaatgtc aggaaaaatc
      901 gtaatgaatt attaaaggaa aaacgaagcc caagaagatt gtttaaataa acatattcta
      961 aatattttta aaccaaattt tatttttcca ctggtgaaaa aaaattcact tttatacatt
     1021 gtcagaaact ctaaagttca tggaaaggga gtatttgcaa gaaaagatat tcctaaggga
     1081 acaaaaataa tagaatatgt gggaaatata gtttcttcta aagaaggact aagaatatat
     1141 gaagaacaat taaaaaaatc caaagaaaca ggtgtgggag cagtttacat ctttcaatta
     1201 aacagaaaac aagatatcga tggagatgtt ccttggaatc ctgcaaaata cattaatcat
     1261 tcttgtaatc caaactgcaa atacaaaata atcaataatc atatttggat attttcaata
     1321 agagacataa agaaaggaga agaattaaac tatgattatg attacgattt agataattat
     1381 caagaccacc tctgtaaatg taattcaaaa gattgtttag gttttataat cggaaaaaga
     1441 tacagaaaaa aatttaagaa gctaatgaaa ataaatctta atatttataa agtatatatc
     1501 cattagtact ctatgaaaac ctcaactaag attataattg cagttgttgt aatagttgta
     1561 gctgttttaa tttgggggct tgttggaagc tctgaagctg caaaaattgg aacaacctgt
     1621 gattttggta ttggagaaga tggaagtgtg ctatgctgga aatggcatag aaatgcatgg
     1681 ggccaaacag gagatgctat taacagttgg cttgaaggaa aataattgtt tttataaata
     1741 tcctgtatct taatagaaaa tgttaaatga aattatattg gctttaatac aatctctaac
     1801 agaatttttg cctatttctt ctgacggaca tttagcactt gtttcaaata ttattgggac
     1861 accaaatata ttctttataa ctttccttca tttagcttct ttgtttgcag ttttaatttt
     1921 cacaagaaaa gaaatatcag aaattttaaa attcaacaaa gaaagttgga gattaattaa
     1981 atatcttata ataggaataa ttcctgcagg attagtaggt ttatttttaa gtgatttaat
     2041 tgaaagcact ctaacctctt actttttaat tggaatattt ttctttttta caggaataat
     2101 tgtattctca actaaattca taaaaccatc taaaaataaa ctctcaaata aaagtgcatt
     2161 ctcaatagga ttaatgcaag ctcttgccct ccttccagga gtctcaagaa gcggtatgac
     2221 tatttcaaca gcaatgttct ttggaataga aaaagaaaaa gcagttaagt tttctttttt
     2281 gatgtttata cctttaacac tagcagcatt tatatttgag acaattaaat attctgcaga
     2341 agttatgaca atacctgcac ttactttgat tgtttcattt acagtttgtt tcttatttag
     2401 tttattattt ttgaatcttc ttaactatat tgtgaaaaaa gataaattct ggttatttgg
     2461 aatatattgc tttttaatag gactagttag tttgattatt tattttattt aaaacaattt
     2521 attgagcttt tgtagggtca gtcattcttc ccatgaaaag tatgtttcct gtttcttttt
     2581 cttgaattat aaatataaat ggatggtctg ctctaaagac atttgaaggc attgcagatt
     2641 taaatcctat cataattgaa gttgctgcag cagcttctgt ccccttttca tctacttgaa
     2701 caaatgcttg atgaatgaca ttttgaatgt ataaattttg tgaaccatca ataccagata
     2761 aatctgcttg catagaaaaa gcagtaggca tacccatttc acttaatgtt gaaaccataa
     2821 agtattttgt atcaaattta aatttaggta aataaattgc acctaacttt gtttcttgca
     2881 tttcagattt atattcatta agtttttcag aagataattc aatatcttct aagttgtaat
     2941 tgtaagttat tttttctcct ttttcataat catatgcttc tccttgctta ggtaataata
     3001 tcagcattga aattttatct cctttgtaag gtaattcaag aatctgtaat ttttctaaat
     3061 ctgcataatt aaatgttgct ttatctgaat ccatatacat cattggagtt tttacaacat
     3121 tttcaggagt aatataaaaa tttttatcac gagtatcaga tttatcaaat tcccattccc
     3181 atgtcccctt aaagtatatc gcatttgtaa taactaatct agttagataa tcaacctgtc
     3241 ctgctggaat taattcttta actttattat ttgtttgttc ttctataaaa gagtttattg
     3301 tttgccttga cttttctgtt tctttaataa aatctacatt agatgctttt cccccataat
     3361 atctttcaac agcttcagag taatcgttta acaacagata atcttgttgt acccacaagg
     3421 catttcctgt gcttaattga tattccttat tattttcatt aatcttatta tatattgccg
     3481 cataatttgg tcttaataca gaagtttctg ggaaatgaaa cacttctttt atttcttctg
     3541 ctgtttttcc tttagcacct tcataggtca ttgccaatgc agtagatatg ctgtaaggag
     3601 aataaaaaat attgtctttt tcagtttttg ctagttcaga atataaatca aaagcaaact
     3661 ggttgtttgc atttacaacc tcttgtattc ctttaggtgt tgaaccaaca tcatctgccc
     3721 taggaggttc atcaggatta taaggaaaca aaaataaacc tactgtaaca gcagttgcca
     3781 ccccaattac tattaatata ataactaata cacttacagt ttttttgttt gctgtttttt
     3841 gcataataaa taaatgaata acctatttat aaatattttt taaagttcaa ttttatttga
     3901 agttataatt taataagatt tattaaatca ttattcttgt ttttaatatg gttaagaaaa
     3961 aaccaagtaa aaaatttgtt aaagcaaaga ttaaaccttc tcataaaagt aaaaaaccaa
     4021 atcctaaaat ttttgagtcc tatgatgata aaaaaagagc agagtggaat aaggagagag
     4081 gaaaatttga agaaattgca gaggaacttt ctagagatag tgatttcata ggattatata
     4141 ataatatttt gagagaaaaa tgggttaaac aaaattatcc tcgtcaaaaa gatactaaag
     4201 ctttagttag aagagcaatg gacttataca ggataagaat aaagaaattt tctatataat
     4261 tttatttatc ttccagaata tctaagaaat tatttaaaca actcagttaa atctattccc
     4321 tgagaagtaa tttttctgta ttcctctatt tctttgtctt caattttttt gaaaatcaat
     4381 tcaggtttgt ttatttcgtg ttcttttcca aagtcctttt cagcttcttc ccatgctcct
     4441 gaacaactag gacttccttt gaggtttaat tgtttccata ctttttcaga ggttttgggc
     4501 aagtaaggtg caaaaagtat tgcaagattt ttcaacaaaa aagcacttat ataaaaaata
     4561 ttatttgctc tctttggatt ttcttttata acattccacg gctcactatc attaatgtat
     4621 ttgtttcctt cacttgacaa ttctagaatt tcagcataag cttttcttaa ttcagctctt
     4681 tccattaagt cccctatgct ttttcttttt tcctcaattt ttttcaatag attcttatca
     4741 atttcctgca attctttaga tgcaggtttt gttatctttc tgtctaattt tgtatatata
     4801 aaattcaaaa ctctatttac ataatttgca taatttccaa ttaaatctga attaactctc
     4861 tcttgaaagt ctttccatct aaattcagaa tcacttgtct caggtattat ctgagttaaa
     4921 taatgtctcc aaacatctgc ttcaagtttc aattcaggga gcctttcaca gaaaacgcca
     4981 attcctttac tcttagaaaa cttctgtcct tcataattaa gatattgcaa tccaactaca
     5041 ttcttaggta aattaaattc accattagca ataaccattc ctggccagaa aattgtatgg
     5101 aaaggaatat tatctttacc taaaaaattg tatacttctg atttttcatc tttccagaat
     5161 ttttcccagt cctttgttat ttcttttgtt gatgaaacat atcctatggg agcatcaaac
     5221 caaacataaa agaccttttc cttaaattct tttaatggaa cttttacacc gtgctttaaa
     5281 tctctggtaa tacatcttgg tttcaatccc tcatttatcc aacccatagc taaattcctt
     5341 acttgctctc taaaatattt attagattga atccatttct taagtttagg tgttaaactg
     5401 tctaatttca aaaacaagtg ttctgtttct cttatttcaa gttttccacc agatattgtt
     5461 gattttggat ttttgagttg agaggggtct aaaacagaag tgcatttttc acattggtcc
     5521 ccgcttgcat caggatatcc gcattttgga cactctccaa caacatacct gtctggtaaa
     5581 aacatatttt cagtaggaga ataaaaaacc ttcatcttgt ttttttcaat aaatcctttt
     5641 ttatttattg tttcaaaaaa ttctttaact gtctcatgat gtattttttt acttgttctt
     5701 gaaaaactat cataagatat ttcaaaccag tcatatattt ttttgtgttc tttgtataat
     5761 ttatctgcaa atttttctat tggaacattt aatttaaatg cagcaatctc agaagttgag
     5821 ccgttttcat ctgttcctcc aatatagagt gtttcataac ctcttgccct agaatatctt
     5881 gcaaatatat ctccaggtaa gtgagacccg actatatgcc ctaaatgagg aacattgtta
     5941 atataaggta atgcagaagt tatcaacctt ctattctttt ttttgggttt aatattttta
     6001 accatacatc ttaaatccct cttatgtttt taaagatatt cttacactta taatctcctt
     6061 aaccgttaat attaaaaata acaaaatctt atataattca tggaagaggt gtttaataca
     6121 gaagaagaaa gtaatgcttt caacaggagt caagagagtg aagtatttag tacatctaaa
     6181 ggacatcaag tagtaagcac agaacgatta gaaccaaaga tagttgagag aagaatctgg
     6241 aacttaaatc atatttttct tggacttttt attgcaatag ttttcattgc aataggattt
     6301 tttttaatca tcaattatga tttggattta acaaattcac ttatagttgt tttggttatt
     6361 gttgtagtat attcttcaat tttatttttc ttaatagaac caaaagtatt aagacaggta
     6421 gataacactg ttgtccagag agttgaaaga cccgttctta tagaaaaacc agttgttaaa
     6481 gaagttcctg ttgaaaagaa aatatttgta gacaggccag tagaagtaat aaaagaagtt
     6541 cctgttgaaa aagagattat taaggaaatt gaagtaccaa tagaaaagac agtatatatt
     6601 gacagaccag ttgagaaagt tgttgaagtt ccttcagaac caactattat tacagttcct
     6661 caagaaacca aaaaaagaac cccttctttg aaatattctt ttgttgcttc agaagaaaca
     6721 cgagtttacc atactaaaaa ttgtagactt ggcaaactta tcaaaaggaa atacaaactt
     6781 gtttctgatg atgaaaaaga tttcataaaa gcaaaattca gaccttgcaa agtatgtata
     6841 aagaaaacaa aaaaggttta gttaaataat taatcttata aagctcttta ttatttattt
     6901 tattgaagag gaaagatgca aagtataaca gaatcattgc aatccatgaa tttaactggt
     6961 ttaactgaaa gagaagccag ttcaagatta caaaaaaacg gatacaatga gcttccaaga
     7021 gaaaaaagaa aaacaacttt taagattgta ttggaagtcc ttaaggaacc aatgtttcta
     7081 ctcttgattt tttgctggat aatttatttg attttaggag atataaaaga atcttttttg
     7141 cttttaggat ttgttgttgt tataatattc ataacaattt atcaggaaaa taaaacagaa
     7201 aaagctttgg atgcacttag aaatttatct agcccaagag ctctggttat aaggggagga
     7261 caaagaaaaa gaatacctgg aagagaagtt gttcagggag atataattgt tctttctgaa
     7321 ggagatagag ttccagcaga tgcaattgtt gtgacttcaa ctaatttttt tgttgatgaa
     7381 agcattctta caggagaatc tgttgctgct aaaaagaaac caacaaaaaa cttcaagata
     7441 ctcatgcaaa agccaggagg agaaaatact ccttttattt attctggaac tttagttgtt
     7501 aggggaacag caatagcaat tgtaaaaagc acaggaattg aaacagaaat gggaaaaatt
     7561 ggaaagtctt tagaagttat aaagacagaa aaaaccaatt tacaaaaaga aacaaattct
     7621 ttagttaagt attttgcaat atatggaata agtgcctgcg ttctggttat tatagcttat
     7681 ggtatcttaa aaggaaactg gattgaagct atgctcagtg gagtaactct tgcaatgtct
     7741 gttcttccag aagagttccc tgttgtttta actttatttt taggattagg ggcttggaga
     7801 atgtccaaga aaaatgtttt aataagaaga caacaggcaa tttcagaatt aggttcaaca
     7861 actgttcttt gtactgataa aacaggcact ttaactcaaa acaagatggc agttactaaa
     7921 ctttattgga acaatcaaac ttgctttatt gaaaacaata aaaaaatacc agaacaattc
     7981 catgaggttg ttgaatatgg aatattagca agtcaaaaag aaccttttga cccaatggaa
     8041 aaggccttga aagaattatc cagaggaaat gaatctaaat ttattcacaa gacttggcat
     8101 cttttacaag agtatccttt atcagataag ttaatggcaa tttcacatgt ttggtcaaaa
     8161 agaggaaaat cagaaaatat agttgcagct aaaggagcac ctgaagcaat aatagaacta
     8221 tgtaggatgg attttaacaa caaaaaaaga atcttaaaag aagttgcttc tttttcaaaa
     8281 gaaggattaa gggttattgc tgtagcaaaa tcttcttcaa ttgggccttt accaaaagac
     8341 caacatgagt ttgattttga gtttgtaggt ttaatcggat ttgaagaccc aatacgtcca
     8401 actgttcctc aagcaattaa agaatgttat gatgcaggaa taagggtcat aatgattaca
     8461 ggagattatc acggaactgc ccagaatatt gcacttgaaa ttggattaaa aaacccagat
     8521 aaatatatta caggagatga acttgaaaac ctatctgatt ctcagttaaa aaagaaaata
     8581 aaagatgtaa acatatttgt aagggttgtt ccacagcaga aattaagatt agttaatgct
     8641 cttaagtcaa atggagaaat tgttgcaatg acaggggatg gagtcaatga tgctcctgct
     8701 cttaaatctg ctaacatagg tgtttcaatg ggagagagag gaacagatgt tgcaagagag
     8761 gcctcttcaa ttgttctttt agatgataat ttcacttcaa tagttaatgg tgtgaaaatg
     8821 ggaagaagag tttttgataa tataagaaaa gcaatgtctt atgttttttc aatgcacata
     8881 cccatattag gtataactgt tttagccctt atttttggat ggcctttaat cttgttacct
     8941 gttcatattt tatttatgga attaataata gacccaactt gttcaattgt ttttgaagct
     9001 gaaaaggcag aagaaaacat aatgaaaaga aaacctcgta atccaaaaga aaaaatattt
     9061 aataaaagaa ctttaaatca tagtatcttt caaggtttag gaatgcttct tagtatggct
     9121 gctttgttct acttcacatt taaatccaca aacaatgaat atcttgctcg tgcaatggct
     9181 ttttctaatt taattttctc aagtttactt ttaattttaa gcagcagatc aatctctaaa
     9241 ccattttaca aaactatttt agaaaaaaac aaagcattct ggtttgttgc tataggtgca
     9301 ttattctttt tggctctatc tttgtatacc ccatttttca atgaggtatt tagatttagt
     9361 acattaacaa taaaggaatt gggaatttca tttggggttg ctttattagg tatgctttgg
     9421 tttgaaattg ttaagcatac ccattatttg cattatgaca aaaaaagatt tattccaaaa
     9481 taaatttaga ttagatattt ataa
//
LOCUS       DAOPCH010000032         9466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_6877_length_9466_cov_33.979492, whole genome shotgun sequence.
ACCESSION   DAOPCH010000032 DAOPCH010000000
VERSION     DAOPCH010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 9466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9466
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6877_length_9466_cov_33.979492"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..579
                     /locus_tag="PLU55_03145"
     CDS             217..579
                     /locus_tag="PLU55_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87082.1"
                     /translation="MLCRHLHPKTNIICVRNGDRYVPVNSRESFMLYLGMLSSVAYRK
                     FINAAMDINLLFMIEAYNDKRYYVINPVFATNRRVNFNAITTFFSIISSFGNAVTIDE
                     KYVEDIIKPESDKRVDRW"
     gene            573..>1100
                     /locus_tag="PLU55_03150"
     CDS             573..>1100
                     /locus_tag="PLU55_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87083.1"
                     /translation="MVKDLETKEVDLYLLAQARTSFIISLQKPSYRELEKSYNIPKST
                     LFYFAKKEDWETQRVEYHQKVIEKSQNSLKAKVSRERSKKLVKVNDIFHKGADKLIQQ
                     LNENQYRMTVKDLNVLARLAEFLAGEVEARKEHTFKLDKPLSEYTVEELLAMQRNIMD
                     GNAEVIDIEDAEYEEL"
     assembly_gap    1101..1200
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1201..2694
                     /locus_tag="PLU55_03155"
     CDS             <1201..2694
                     /locus_tag="PLU55_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87084.1"
                     /translation="VTLEDINQEIIRREIQKRQEKNYLRDNLWEYCKEYDPDFFIDDK
                     TLLKDVATKLQLVKEGKIKKLAISIYPRAGKSYLVSNYCAWILGNHPTGCVMRNTYGD
                     HLAHKFSYDVRQMIRSDLFKTAFEGIKLSKSKKSLDCWALTTSKQFAYFGSGVRGTTT
                     GYGCNLAGIVDDPIKNMADAMSPNQLEIVWDWYYSIQKTRYESNPDTGVECPTIVIST
                     MWSKGDLINRLIDLQGTVEEGGLWTFVSYPALNDNDESSCEAMMSTEKLHTLRDEYYV
                     AGEEFIWETMYMNKVVQKYGALFPKSSLCFLDQELFPKDYDAIIGWCDPADKGTNWTC
                     SVIIGIKNGLAYILDIIYTRKSYEYYKPLLVEQIIKFLPNYYIVESNKDGRIIAVEIR
                     QEVGKILDNMRIERPDKVFEVTIGTKVQSKNKELRIMLNSNLIKTMFIFPRLSYNNNF
                     YGMFIKDLTEYLAEGNNEFDDAPDTLAGLASLIKPKNSALVEVINAR"
     gene            2684..4015
                     /locus_tag="PLU55_03160"
     CDS             2684..4015
                     /locus_tag="PLU55_03160"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HPJ87085.1"
                     /translation="MPDNNIQSIPDKVYIFNTAKRDILPASAAIPLEAKNALTQYGCV
                     IPPIDRAFLPMLEALDPTHKSSIDVKSIATSQKGYKCSSNNRKLNTFLHKPNYDSFKT
                     FQDIVDAFTYEFYVYDEAYLELIRIADRVSIFNTPVRYVYVKVNKQGRIDKYCYVTDD
                     GEVVEFEPYNGGELKNGVRYLVGMRNYNTASYFYGYPAYMSALEAMLENSYIRRYGTT
                     FFNNNATPDKALILKGTLMSKENKESIKNYMTDNYKGIDNAHRLLIITLDDEGSEADF
                     VDISSSFNSSFLEEYRKNRDEIIAVHQIPPKLMGITVASGLSTGTDTIGSLRDFVERT
                     IAPRQNKISNFFSTLLSEIFALDVKFSLLQVDTTNDKDDAIINNIYANIVDDEGKPVK
                     TVTEIRAEKGYPVKPEGALHGNNNKKAPNTGSVPGQNTYEHPNDIDSARGR"
     gene            4018..4893
                     /locus_tag="PLU55_03165"
     CDS             4018..4893
                     /locus_tag="PLU55_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdF-like putative serine protease
                     domain-containing protein"
                     /protein_id="HPJ87086.1"
                     /translation="MAIRRRKLKNLKVEYISYVDKAANLRSFYLTKRYEEEKHKPDFQ
                     MNVKLICNADNEEKKVYGIVYAPNTVDAHGDWTDEKTLEKAAHQFMLDYQKIDSMHNL
                     EEGAGKVIESFIAPTDFVVNNETITKGTWVIATQATDEVWERIKKGELTGYSLYGTAE
                     AEYEKMNLLEIIKALLQGKTLTIESPMEVTMDEELKKALTNLLELSETVKKMDGNIAS
                     MQDVLVKGKEVLDKFANLKVEEIEKSFNTKIDEKLVELNKKLDSIIEAISTDTTAKTA
                     NNKQDEVTAYKSSIL"
     gene            4902..6863
                     /locus_tag="PLU55_03170"
     CDS             4902..6863
                     /locus_tag="PLU55_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87087.1"
                     /translation="MGEELIQQLIKALDSAMPKRRNVAKNYVNPLAAMMLEQAGYQVK
                     LRDGTIWKHEYTTNYGFAEFTRARHLQGLEAKESMLLINSQSWFLDRYNKLTGDSSII
                     PMDFWTGIPEQLTDTQRITSTPQDNANVVASRNKWANMFGDEVYVHHLERQVDLPIEV
                     IRQYLYDPDFEARVEKIFGNEIANDLVRLAVNGNDVSFASGDFYKLLKSYATLLKEAK
                     GTKTLVSGLSRFVGKHGNLITPVKVNAPAIRFIDPFSDNFSADTSANYTASAGALAVS
                     GGLLKWTGTSWTGGTVRYNNVIPVLMNTNYVASIYIKPGATSSCYITIKDPAGNTIAV
                     SNTASIGTDGGTIQVAFNSYNNLGVVFNVTCTQSSGDTDYDLDNVSIKRSVNKFEPYD
                     LLNILDVMIDHYNPEYEVADDELAFLVSKEDASLIAKGLRIPVYVTDKGQLIPMATET
                     RESRTVETQGAIQYRGYPIVVVPYAKSLNKGGFIIFGADKAEYRIAIQTLFSYNRVYN
                     PRMEKGGEGYQYTYHFYQAYGVRNPGKFVIAEGIGSSLACEDLIFATSKDLIGSRIGS
                     SASAVTIDKSVLAAAGAKGAFVFCDTPGAEIFYSATSGNLDTYALASTQTPAEGVDFA
                     SLTNGTYYVRAFLDGVTLPSTKMTVTVQD"
     assembly_gap    6968..7067
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7221..7697
                     /locus_tag="PLU55_03175"
     CDS             7221..7697
                     /locus_tag="PLU55_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87088.1"
                     /translation="MAFSTTTVLSKKVWDVWVYCSAVAEYQSSPGVNVGQLDGDASLK
                     VSPGDTLKLASGIEATISENGEATFTIVGHGETAAAFKNNYESLKGLINKSCNVAFVP
                     AGTTSGNFGTENVKLSNVYLFPELNIQANQVNKITVTAKRETGVGTAVTINATNNS"
     gene            7768..8628
                     /locus_tag="PLU55_03180"
     CDS             7768..8628
                     /locus_tag="PLU55_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87089.1"
                     /translation="MSFTQTTLLNKAGYFAFLSKDNLAEDYDPVEVVSLINAAQYIAR
                     NLLGTTPIMVTSGSEIMVDANNPPQSLLYCCKALPSDVMISTNNLRSAFVILNDSELY
                     NIDIGIDTQTAYTLAPGQMILIVTSPYKTSVTPNYLIFEGVIPYGWTAEDMSLKVGKG
                     DEVRISTGDTMVQSAITEVNLPGYEQFSFFTNGKPSAIDSAYTVTDIDLGLIPASLRY
                     LLIVPVLPYNEFVDIDGVYAGVPIYQIDLLNGVTISQEWNANQPNKSTLSFKKDAGFN
                     EEIAVPIYVE"
     gene            8640..>9466
                     /locus_tag="PLU55_03185"
     CDS             8640..>9466
                     /locus_tag="PLU55_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87090.1"
                     /translation="MSFTKTTNLTKARLFALGSYYKTRTSNHLLPETTKMFSAIIPYY
                     LLHQFDEPIFVNAGEDDITVSNVDTGYYILDGNFENTITFPNTDTPYCCFIVNGDNYS
                     TLLKIGGTTLSLDDYPGAVILIANGKFPGIDNRQTIMIPYIFPIGVAYEDLSLKELKG
                     DELPLSFGMQAVISKKVELQLPIFEMLHRDKSWTYLNRYLTDYYSAWLYSSAMDTIYV
                     LQLPDKDNLLGMVFRFDCGVHKDGIGNQIAQDIISNQSNKFTFTYNREVGVTENIIN"
BASE COUNT         3131 a         1359 c         1836 g         2940 t
ORIGIN      
        1 gggataaaaa taagtcttga cataaaagat aatgtttgat tttaagactt agttagatat
       61 attatatcta aggagactaa aaaaatgagt aacaacctac ccaaaaacta taggtgtaag
      121 tcactgacaa gtaagctttt agctactata cctaacgata tattcgttta tgtgcaggct
      181 ccatttgagt ctgtgattta taataagtat tatctgatgt tatgtaggca tcttcatcct
      241 aaaaccaata ttatttgtgt tcgtaatgga gatagatatg ttcctgtaaa tagtagagaa
      301 tcttttatgc tttatctggg aatgctaagt tctgtagctt atcgtaaatt catcaatgca
      361 gctatggaca tcaacttatt gtttatgata gaagcttata atgataagag atattatgtt
      421 atcaatcctg tctttgctac taacaggaga gttaatttta atgctatcac tactttcttc
      481 agtatcattt cctcttttgg taatgctgtt accatagacg agaagtatgt agaagacata
      541 attaaacctg aaagtgataa aagagttgac agatggtaaa agatttggag actaaggaag
      601 tagatttata tttgttggca caagcaagaa cttcttttat tatttctttg caaaaaccat
      661 catatagaga attggaaaaa agctataata ttcccaaatc tactcttttt tattttgcta
      721 aaaaggaaga ttgggaaact caaagagttg aatatcatca aaaggttatt gagaaatctc
      781 aaaactcctt aaaagctaaa gtttccagag aaagatctaa aaaacttgtt aaggttaatg
      841 atatcttcca taaaggtgca gataagttaa tacaacaact taatgaaaat caatatagga
      901 tgacagttaa ggatttgaat gttttagcca gattggcaga gttcttagct ggagaggtag
      961 aagcaagaaa agaacatacc tttaagttag ataaaccttt atcagaatat actgtagaag
     1021 aacttttggc tatgcaaaga aatattatgg atggtaatgc agaagttatt gatattgaag
     1081 atgccgaata tgaagaattg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 gtaactttag aggacataaa tcaggaaata attaggcgag aaatccaaaa aaggcaggaa
     1261 aaaaactatt taagagacaa tttatgggaa tattgtaaag aatatgaccc agatttcttt
     1321 atagatgaca aaactctctt aaaagatgta gcaactaagc tgcaacttgt taaagaaggc
     1381 aagattaaga agcttgctat ctctatatat cccagagctg gtaaaagtta tctggtttct
     1441 aactattgtg cttggatatt gggtaatcat cctacaggtt gtgttatgcg taatacttat
     1501 ggagatcatt tggctcataa attctcctat gatgtcagac aaatgataag gtcagactta
     1561 tttaagactg cttttgaagg tattaagctt tccaagtcta agaaatcatt ggattgttgg
     1621 gctttaacta ccagtaaaca atttgcttat ttcggttcag gtgtaagagg aactactaca
     1681 ggttatggat gtaatttggc aggtattgta gatgacccta ttaagaatat ggcagacgca
     1741 atgagcccta atcagttaga gatagtttgg gattggtatt attctattca aaagaccaga
     1801 tatgaaagca atcccgatac aggtgttgaa tgtcctacca ttgttatttc tactatgtgg
     1861 agtaaaggcg atcttatcaa tagactgata gatttacaag gaactgtaga agaaggtgga
     1921 ttatggactt ttgtttctta tcctgctttg aatgataatg atgaatccag ttgtgaagct
     1981 atgatgtcaa ctgaaaaact gcatacctta agagatgaat attatgttgc aggagaagag
     2041 ttcatttggg aaactatgta tatgaataag gtagtgcaga aatatggtgc tttatttccc
     2101 aaatcatctc tttgcttctt agaccaagaa ctgtttccta aagattatga tgccattata
     2161 ggttggtgtg atccagcaga taagggaact aattggactt gttcggttat cataggtatt
     2221 aaaaatggtt tagcttatat cttggatatt atttatacca gaaagagtta tgaatattat
     2281 aaaccattgt tagtagaaca aataattaag ttcttaccta actattatat tgttgaatct
     2341 aataaagatg gtagaataat agcagtagaa atcagacaag aagtaggtaa gatactggat
     2401 aatatgagaa tagagagacc tgataaggtc tttgaagtta ctattgggac aaaagttcag
     2461 tccaaaaata aagagttaag gataatgtta aactctaacc taattaagac tatgttcatc
     2521 tttcccagat tgtcctataa taacaacttt tatggtatgt ttataaaaga cttgacagaa
     2581 tatctggcag agggaaataa tgagtttgat gacgcaccag atacattggc aggtttggca
     2641 agtttgatta aaccaaagaa tagtgcatta gtggaggtta taaatgccag ataacaatat
     2701 acagagcata ccagataagg tatatatctt taatacggct aagagagata tattacctgc
     2761 aagtgcagct ataccattgg aagctaagaa tgctttaacc caatatggtt gtgttatacc
     2821 tcccatagat agagcatttt tgcctatgtt agaggcatta gacccaactc ataagagttc
     2881 cattgatgtt aagagcattg ccacttctca aaagggatat aaatgcagtt ccaataatag
     2941 aaaattaaat acctttttac ataaacccaa ttatgatagt ttcaagacct ttcaagatat
     3001 cgtagatgct tttacttatg agttttatgt ttatgatgaa gcctatttgg agttgattag
     3061 gatagcagat agagttagta tctttaatac acctgtcagg tatgtctatg ttaaagtcaa
     3121 taaacaaggc agaattgata aatattgtta tgttaccgat gatggagaag tagtagaatt
     3181 tgagccttat aatggtggtg aattgaagaa tggagttaga tacttggtag gtatgcgtaa
     3241 ctataatact gcttcttatt tttatggtta tcctgcttat atgtcagcat tggaagcaat
     3301 gttggagaat agttatattc gcaggtatgg gactactttc ttcaataata atgctacacc
     3361 tgataaggct ttaatcttaa aaggaacact tatgtctaaa gagaataaag aaagtattaa
     3421 gaactatatg acagataact ataagggtat tgataatgct catagattat tgataattac
     3481 tttagacgat gaaggctcag aagcagattt tgtagatatt tcttcttctt tcaatagtag
     3541 ctttttggaa gaatatcgga agaatagaga tgagattatt gccgttcatc agataccacc
     3601 taagcttatg ggtataacag tagcttcagg tctatctaca ggaacagata ctataggaag
     3661 tttaagggat tttgtagaga gaactattgc accaagacag aataagattt ccaatttctt
     3721 ttctactctt ttgagtgaga tctttgcttt agatgttaag ttcagtttat tacaggtaga
     3781 tactaccaat gataaggatg acgctattat caataacatt tatgccaata ttgtggatga
     3841 tgaaggtaaa cctgttaaaa ctgtaacaga gattagagca gagaagggtt atcctgttaa
     3901 acctgaaggt gctttacacg gaaataataa caaaaaagct cccaatacag gttcagttcc
     3961 aggtcagaat acctatgaac atcccaatga tatagattca gccagaggga ggtaaagatg
     4021 gctattagac gaagaaaact taagaactta aaggtagaat atatctctta tgtagataag
     4081 gctgccaatt taagatcttt ttacttgaca aaaagatatg aagaggaaaa gcataaacct
     4141 gactttcaga tgaatgttaa gcttatctgt aatgctgaca atgaagagaa aaaggtttat
     4201 ggtattgttt atgcacctaa tactgtagat gcacacggag attggactga tgaaaagacc
     4261 ttagagaaag cagctcatca gtttatgttg gattatcaga aaattgatag tatgcataat
     4321 ttagaggaag gtgcaggcaa ggtaattgag agttttattg ctcctacaga ttttgtagtc
     4381 aataatgaga ccataactaa aggaacttgg gtaattgcta ctcaggctac agatgaagtt
     4441 tgggaaagaa ttaaaaaagg agagcttaca ggctattcct tatatggaac tgccgaagct
     4501 gaatatgaaa agatgaatct tttggagatt ataaaagctt tgctgcaagg caaaacatta
     4561 accatagaaa gtccaatgga ggtaacaatg gatgaagaac tgaaaaaagc tttgacaaat
     4621 ttgttagagc tgtcagaaac agttaaaaag atggatggta acatcgcatc tatgcaagat
     4681 gtattagtaa aaggcaaaga ggttttggat aagtttgcca atcttaaggt agaagagatt
     4741 gagaaatctt ttaatactaa gattgatgag aaactggtag aactcaataa gaaactggat
     4801 agcattattg aggcaatttc taccgataca actgccaaaa ctgctaacaa taagcaggat
     4861 gaagtaactg cttataagag ttccattcta taggagaaaa aatgggagaa gagttaattc
     4921 aacagttaat taaggcactt gatagtgcaa tgcctaaaag acggaatgtg gctaagaatt
     4981 atgttaatcc tttggctgca atgatgttag aacaggcagg ttatcaggta aagttgagag
     5041 acggaactat ctggaaacac gaatatacca ccaattatgg ttttgctgag tttaccagag
     5101 caagacatct acaaggcttg gaagctaagg aaagtatgtt gcttatcaat tcccagtctt
     5161 ggtttttgga caggtataat aagctaacag gagattccag tattattcca atggatttct
     5221 ggacaggtat tcctgaacag ttaaccgata ctcaacgcat aacttctaca cctcaagata
     5281 atgccaatgt agtagcctct cgtaataaat gggctaatat gtttggtgac gaggtctatg
     5341 ttcatcattt ggagagacaa gtagatctgc ctattgaggt tattcgtcaa tatttgtatg
     5401 acccagactt tgaagcaaga gtagagaaaa tctttggtaa tgagattgcc aatgatttgg
     5461 tcagacttgc tgttaatggt aatgatgtca gttttgcttc tggagatttc tataaactcc
     5521 tgaaaagcta tgctacttta ttgaaagaag caaaaggaac taagacctta gtttctggac
     5581 ttagcagatt tgtaggtaaa cacggtaatc tgattacacc tgtgaaggta aatgcacctg
     5641 ctatcaggtt tatagatccc ttctcagata atttctctgc agatacttct gctaattata
     5701 ctgcttctgc aggtgcttta gctgtatcag gtggattact taaatggaca ggaacatctt
     5761 ggacaggtgg aactgtcaga tataacaatg ttatacctgt gttaatgaat accaactatg
     5821 tagccagtat ttatattaaa cctggagcta ccagtagctg ttatattact ataaaagatc
     5881 ctgctggtaa taccattgca gtttccaata ctgcctctat aggaacagat ggtggaacta
     5941 ttcaagtagc cttcaattcc tataataact taggtgtagt atttaatgtt acttgcactc
     6001 aatcttctgg agatactgac tatgatttgg ataatgtctc tattaagcgt agtgtaaata
     6061 agtttgaacc ttatgatttg ctgaatattt tggatgtaat gatagatcat tataatcctg
     6121 aatatgaagt tgcagatgat gaactggcat tcttggttag caaagaagat gcttctttaa
     6181 ttgctaaagg tttgagaata cctgtctatg taacagataa aggtcaactt attcctatgg
     6241 ctacagagac cagagaaagt agaactgtag aaactcaagg tgctatacag tatcgtggtt
     6301 atcctattgt agtagttcct tatgccaaga gtttgaataa aggtggtttc attatctttg
     6361 gtgctgataa agcagagtat agaattgcca ttcaaacctt attctcttat aatcgggttt
     6421 ataatcccag aatggagaaa ggtggagaag gttatcaata tacttaccac ttctatcagg
     6481 cttatggtgt tcgtaatcca ggtaagtttg tcatagctga aggtattggt agttctcttg
     6541 cttgtgaaga tttaatcttt gctaccagta aagacctgat tggtagtaga ataggttcaa
     6601 gtgcttctgc tgttaccata gataagagcg ttttggcagc tgcaggtgca aaaggagctt
     6661 ttgtcttttg tgatactcca ggtgcagaaa tcttttattc tgctacttct ggcaatctgg
     6721 atacttatgc tttagcttct actcaaactc ctgctgaagg tgtagatttt gctagcttaa
     6781 ctaatggcac ttattatgtt agagcatttc tggatggtgt aactctacct tccactaaga
     6841 tgactgttac agttcaggat taaggagata aataatggct aatacaacta agaaaactaa
     6901 tattaaagca gaagatcctg ccaaagcaga agtaacaggt gtagaaatac ctgtagaaac
     6961 taaggcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaga agagaaagtt
     7081 actccagaag taagagtaaa gttcttattc agatggctta agccagatgg cacaactgct
     7141 gagataggag aaatcctttc tttggatagc aaaattgccg ataatctaat taaatataag
     7201 atagctatta aggaggaata atggcttttt ctaccactac tgtattaagt aagaaagtct
     7261 gggatgtctg ggtatattgc tcagctgttg ctgaatatca gtcatctcca ggtgttaatg
     7321 ttggacagtt agatggagat gcttctttga aagtttctcc aggtgatact cttaagttgg
     7381 cttcaggtat tgaagctact atctccgaaa atggagaagc aacttttact atagttggtc
     7441 acggagagac tgctgcagct tttaagaata actatgaatc gcttaagggt ttaatcaata
     7501 aatcctgtaa tgtagccttt gttcctgcag gaactacttc tggcaatttt ggaactgaga
     7561 atgtgaagtt atccaatgtt tatctctttc ccgaattaaa cattcaggca aatcaggtaa
     7621 acaaaattac tgtcacagcc aagagagaaa caggtgtagg aactgcagta actatcaatg
     7681 ctaccaataa cagttaacca ggttggggaa gttcaatctt ccccttcagt ttttatcttt
     7741 acatagatag acacaaagga gattaaaatg tctttcactc aaactacact cttaaataaa
     7801 gcaggatact ttgccttctt aagcaaagat aatctggcag aagactatga cccagtagaa
     7861 gtagtttctc ttatcaatgc tgctcaatat attgctcgta acttattggg aactacacca
     7921 ataatggtta ccagtggaag tgagattatg gttgatgcaa ataacccgcc tcaatctttg
     7981 ttatattgtt gtaaagcact tccttctgat gttatgatat caactaacaa tcttagatct
     8041 gcttttgtca ttcttaacga cagtgaactg tataatattg atattggtat tgatacgcaa
     8101 acagcatata ctctggctcc aggtcagatg attttgatag ttacttctcc ctataaaaca
     8161 tcagttacac ccaattatct tatctttgaa ggtgttattc cttatggttg gactgcagaa
     8221 gatatgtctt tgaaagtagg caaaggagat gaagtaagga tctctacagg agacacaatg
     8281 gtgcaaagtg ccattacaga agtaaacttg ccaggttatg agcagttcag tttctttacc
     8341 aatggcaaac cttctgctat agatagtgct tatacagtta ccgatattga tcttggttta
     8401 attcctgcca gtctcagata tctgttaata gttcctgttt tgccttacaa tgagtttgta
     8461 gatatagatg gtgtttatgc aggagttcct atctatcaga ttgatttact taatggtgta
     8521 actatcagtc aggaatggaa tgccaatcaa cccaataaga gcactttatc tttcaaaaaa
     8581 gatgcaggtt ttaacgaaga aattgcagtt cctatttatg tagaataaag gagattaaaa
     8641 tgtcatttac caaaacaacc aacttaacca aagccaggtt atttgcctta ggctcttatt
     8701 ataagacacg tacaagcaat catttattgc cagaaactac taagatgttt tctgccatta
     8761 taccttatta tttattgcat caatttgatg aacctatatt tgttaatgca ggcgaagatg
     8821 atattactgt aagcaatgta gatacaggat attatattct tgatggaaat tttgaaaata
     8881 caataacttt tcccaataca gatactcctt attgctgttt tatagttaat ggagataatt
     8941 attcaacgct tcttaaaatt ggtggaacta ccttaagttt agacgattat ccaggtgcag
     9001 ttatcttaat tgccaatggt aagtttccag gtatagacaa caggcaaact ataatgatac
     9061 cttatatctt ccctataggt gtagcttatg aagatttaag tcttaaagaa ttaaaaggag
     9121 atgaattgcc tttatctttt ggaatgcaag ctgttatctc caagaaagta gaattgcaat
     9181 tacctatttt tgaaatgttg catagagata aaagctggac ttatctgaat agatatctta
     9241 cagactacta tagtgcttgg ttatattctt ctgctatgga taccatttat gttttgcagt
     9301 taccagataa agataatctg ttgggtatgg tctttaggtt tgattgtggt gttcataaag
     9361 atggtattgg caatcaaatt gctcaagata taatatccaa tcaaagcaat aaattcactt
     9421 ttacttataa tagagaagta ggcgtaactg aaaacattat caatat
//
LOCUS       DAOPCH010000033         9250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_7058_length_9250_cov_8.235889, whole genome shotgun sequence.
ACCESSION   DAOPCH010000033 DAOPCH010000000
VERSION     DAOPCH010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 9250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9250
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7058_length_9250_cov_8.235889"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..476)
                     /locus_tag="PLU55_03190"
     CDS             complement(222..476)
                     /locus_tag="PLU55_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87091.1"
                     /translation="MANNTGKKYGGRVAGTPNKLTKELRAVLKNILHQEIELLPEHFN
                     KLDPKDRMELLVKLLPFALPKVQPESYQIGEGGLCDDWDI"
     gene            complement(546..1475)
                     /locus_tag="PLU55_03195"
     CDS             complement(546..1475)
                     /locus_tag="PLU55_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87092.1"
                     /translation="MYDTINLWLSIDKASSFDLSKTLQNLSNITEHTRDDGQMYVSGY
                     LKNFKVNISEQGVSLKGSLAKYFLPDNFHTLTRSDSARAFEMMSDELYLPIQIAKVNR
                     IDFSQNFLMDFEPEAYYPFLGECQYYNRQPQPKSLYYSNGVRQKLFYNKIAEGKVKGV
                     SLPDVWNGQNVLRYEMRFISRLPKQFNQAEITASTLSDEKFYITIFDKWLAEYEAIDK
                     LHSINFNLSDMNSPKDFWKQINLMAINMIGQDKIMQEIENLRHQKAFDKPEYYSRLKN
                     EIKALCKTPEMTASSDLVAELDKKIRTAKRHYR"
     gene            complement(1512..2048)
                     /locus_tag="PLU55_03200"
     CDS             complement(1512..2048)
                     /locus_tag="PLU55_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87093.1"
                     /translation="MNYYKFVMLPDEVRAANKIKSKARLDCISFTDMVAGNYKGLTNF
                     VNHKGQLFFYKTPCRDFVNTDSKRLAEWSLTGNGINFSSIYIDDLDYPEIGYGYPNAS
                     RLLSNGSQNPLFEFRNDGYLFLINKDYSEIELLIIPDGRNLISSYYQKFIDGGFDDEV
                     RELRKQAKIFYQYDLLVL"
     gene            complement(2045..2326)
                     /locus_tag="PLU55_03205"
     CDS             complement(2045..2326)
                     /locus_tag="PLU55_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPJ87094.1"
                     /translation="MIDERLKNIESLLSLQKTVLNFDEVAAYTGLSKSYLYKLTCSGG
                     IPCYKPQGKHIYFNKQEIDQWLMQNRKATTKEIDNQAATFVTLRKGGAK"
     gene            complement(2470..3306)
                     /locus_tag="PLU55_03210"
     CDS             complement(2470..3306)
                     /locus_tag="PLU55_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87095.1"
                     /translation="MQNRIDGYFEKLTEFINKIPLSDKGKNYLWFISFCQFYDEPVLS
                     ENDKTTYGIQTEWQLQRFIEQFKKNFQKLRLNAVEKWTDELNRIEPSEAVAKIDKQLY
                     ELRKKELLADIGADGLNDWDRWLQKYLSNEIDYQQKKPTRKPPKQQTKTSYVWQNNPD
                     KELPELYSLMIDNYKLIAPETTCEQFKAVFTGQAIESINPIKWHQDNASELLYFIDRL
                     QKTNNIVHNPKKADYQKMTACFVKPDGKPFKAVWKSLKTNIDINLSSDKQKAIDELVS
                     NF"
     gene            complement(3323..4453)
                     /locus_tag="PLU55_03215"
     CDS             complement(3323..4453)
                     /locus_tag="PLU55_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPJ87096.1"
                     /translation="MKVHIRQRKQSKAGSISLFLEIYKGTTTTPEGKVKNLRDYEYLN
                     LYLIDKPKTPIEKQGNKDTLQLAESIRAKRELEIKNGLYGFTNEFKQSTNFIDYFTDQ
                     MEKRKQSKGNYGNWNSTLKHLIKFAGTKVLFREIDQTFCENFRDYLKNTATTKSGQPL
                     SSSTVSSYFNKFRACLKEAVRKKIILSNPAIDVVNVRVIENKREYLTIDELKAIVKAE
                     CRYDVLKRAFLFSCLTGLRWSDVNNLKWSELQNTNEGWRITFNQQKTKGLQYLDISEQ
                     ARGYLGEQGNPDERVFIGLKYSSYMNVELTKWMMRAGITKDITFHCARHTFAVLQLTL
                     GTEIYTLSKLLGHSELKTTQIYAKIVDEKKREAVNKIPDINI"
     gene            complement(4799..5053)
                     /locus_tag="PLU55_03220"
     CDS             complement(4799..5053)
                     /locus_tag="PLU55_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87097.1"
                     /translation="MANNTGKKYGGRVAGTPNKLTKELRAVLKNILHQEIELLPEHFN
                     KLDPKDRMELLVKLLPFALPKVQPESYQIGEGGLCDDWDI"
     gene            complement(5123..6052)
                     /locus_tag="PLU55_03225"
     CDS             complement(5123..6052)
                     /locus_tag="PLU55_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87098.1"
                     /translation="MYDTINLWLSIDKASSFDLSKTLQNLSNITEHTRDDGQMYVSGY
                     LKNFKVNISEQGVSLKGSLAKYFLPDNFHTLTRSDSARAFEMMSDELYLPIQIAKVNR
                     IDFSQNFLMDFEPEAYYPFLGECQYYNRQPQPKSLYYSNGVRQKLFYNKIAEGKVKGV
                     SLPDVWNGQNVLRYEMRFISRLPKQFNQAEITASTLSDEKFYITIFDKWLAEYEAIDK
                     LHSINFNLSDMNSPKDFWKQINLMAINMIGQDKIMQEIENLRHQKAFDKPEYYSRLKN
                     EIKALCKTPEMTASSDLVAELDKKIRTAKRHYR"
     gene            complement(6089..6625)
                     /locus_tag="PLU55_03230"
     CDS             complement(6089..6625)
                     /locus_tag="PLU55_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87099.1"
                     /translation="MNYYKFVMLPDEVRAANKIKSKARLDCISFTDMVAGNYKGLTNF
                     VNHKGQLFFYKTPCRDFVNTDSKRLAEWSLTGNGINFSSIYIDDLDYPEIGYGYPNAS
                     RLLSNGSQNPLFEFRNDGYLFLINKDYSEIELLIIPDGRNLISSYYQKFIDGGFDDEV
                     RELRKQAKIFYQYDLLVL"
     gene            complement(6622..6903)
                     /locus_tag="PLU55_03235"
     CDS             complement(6622..6903)
                     /locus_tag="PLU55_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPJ87100.1"
                     /translation="MIDERLKNIESLLSLQKTVLNFDEVAAYTGLSKSYLYKLTCSGG
                     IPCYKPQGKHIYFNKQEIDQWLMQNRKATTKEIDNQAATFVTLRKGGAK"
     gene            complement(7047..7883)
                     /locus_tag="PLU55_03240"
     CDS             complement(7047..7883)
                     /locus_tag="PLU55_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87101.1"
                     /translation="MQNRIDGYFEKLTEFINKIPLSDKGKNYLWFISFCQFYDEPVLS
                     ENDKTTYGIQTEWQLQRFIEQFKKNFQKLRLNAVEKWTDELNRIEPSEAVAKIDKQLY
                     ELRKKELLADIGADGLNDWDRWLQKYLSNEIDYQQKKPTRKPPKQQTKTSYVWQNNPD
                     KELPELYSLMIDNYKLIAPETTCEQFKAVFTGQAIESINPIKWHQDNASELLYFIDRL
                     QKTNNIVHNPKKADYQKMTACFVKPDGKPFKAVWKSLKTNIDINLSSDKQKAIDELVS
                     NF"
     gene            complement(7900..9030)
                     /locus_tag="PLU55_03245"
     CDS             complement(7900..9030)
                     /locus_tag="PLU55_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPJ87102.1"
                     /translation="MKVHIRQRKQSKAGSISLFLEIYKGTTTTPEGKVKNLRDYEYLN
                     LYLIDKPKTPIEKQGNKDTLQLAESIRAKRELEIKNGLYGFTNEFKQSTNFIDYFTDQ
                     MEKRKQSKGNYGNWNSTLKHLIKFAGTKVLFREIDQTFCENFRDYLKNTATTKSGQPL
                     SSSTVSSYFNKFRACLKEAVRKKIILSNPAIDVVNVRVIENKREYLTIDELKAIVKAE
                     CRYDVLKRAFLFSCLTGLRWSDVNNLKWSELQNTNEGWRITFNQQKTKGLQYLDISEQ
                     ARGYLGEQGNPDERVFIGLKYSSYMNVELTKWMMRAGITKDITFHCARHTFAVLQLTL
                     GTEIYTLSKLLGHSELKTTQIYAKIVDEKKREAVNKIPDINI"
BASE COUNT         2574 a         1705 c         1443 g         3528 t
ORIGIN      
        1 agtgggttat ttttaattat actcttttat ctgcgaatta ttttcctata caaaaagtga
       61 agtaatttta ttaaagtcct gatattgaaa gtctttgcct gaatttatat agaaaaaaaa
      121 gtatattagc agcaaaaaca gcctatttat tacttttgag ttgcaaagat agttatttta
      181 aaaacatctt tagtgatttc tctaatcatt tagcctctgg tttatatgtc ccaatcatca
      241 cacaaaccac cctcaccaat ctggtaactt tctggctgta cttttggaag tgcaaaagga
      301 agtaatttga ccaaaagttc cattctgtct ttagggtcta atttattaaa gtgttctgga
      361 agcagctcta tttcctgatg caatatgttt ttaagtactg cccttaattc ttttgtcaat
      421 ttgtttggtg tgcctgccac tctgccacca tattttttac ctgtattgtt cgccataatc
      481 aaaccattta aaactacttt agaatattat actatttatt tctaattact ttctaattaa
      541 atccattatc tgtaatgtct ttttgctgtt ctgattttct tgtcaagctc tgccactaaa
      601 tctgatgatg ctgtcatctc tggtgttttg caaagcgctt ttatttcatt ttttaatctg
      661 gaataatact ctggcttgtc aaaggctttc tgatgcctta aattttcaat ttcctgcatt
      721 attttatcct gaccaatcat atttattgcc atcaggttta tttgcttcca aaagtcttta
      781 ggactattca tatctgataa attaaagttt attgaatgta gcttatctat tgcctcatat
      841 tctgcaagcc atttgtcaaa gattgttata taaaattttt catctgacaa ggtgcttgct
      901 gtgatttctg cctgattaaa ttgctttgga agtctgctta taaatctcat ttcatacctt
      961 aaaacattct gaccattcca aacatctggc aaactcactc ctttaacctt gccctctgct
     1021 attttgttgt aaaatagttt ctgcctcact ccatttgaat agtaaagact ttttggctgt
     1081 ggctgcctgt tataatattg gcactcacct aaaaaaggat agtaagcctc tggctcaaag
     1141 tccatcaaaa aattttgtga aaagtcaatc ctatttactt tagctatctg tataggcaaa
     1201 taaagctcat ctgacatcat ttcaaaggct ctggcactat ctgaccttgt caaggtgtga
     1261 aagttatcag gcaggaagta ttttgcaagg ctgcccttta aagacactcc ctgctctgat
     1321 atatttactt tgaaattttt cagatagcca cttacataca tttgaccatc atctctggtg
     1381 tgttctgtaa tgtttgacaa gttctgcaag gtttttgata aatcaaaact gcttgccttg
     1441 tctattgata gccataaatt tatagtatca tacataggct tttgattaaa tttgtcaatt
     1501 aaaacaactt actataagac aagtaaatca tattgataaa agattttggc ttgtttcctt
     1561 aattctctga cctcatcatc aaagccacca tctataaatt tttgatagta tgatgatatt
     1621 agatttctgc catcaggaat tataagcagc tcaatttctg aataatcctt atttattaaa
     1681 aataagtagc catcatttct aaattcaaat aatggatttt gactgccatt gctcaaaagc
     1741 ctgcttgcat ttggatagcc atatcctatt tctggatagt ctaaatcatc aatataaata
     1801 ctactaaaat tgataccatt gcctgtcaaa ctccattctg caagtctttt gctgtctgta
     1861 ttgacaaagt ccctgcaagg tgttttataa aagaataact gccctttgtg attgacaaaa
     1921 tttgtcaagc ccttgtaatt gcctgccacc atatcagtaa aggaaataca atctaatctg
     1981 gctttgcttt taattttgtt tgcagctctc acctcatcag gaagcattac aaatttatag
     2041 taattcattt tgcacctcct tttcttaagg ttacaaaagt agctgcctga ttgtcaatct
     2101 ctttggtagt ggctttccta ttttgcataa gccattggtc tatttcttgt ttattgaaat
     2161 agatatgctt tccctgtggc ttgtagcaag gtatgccacc gctgcaagtc aatttgtaaa
     2221 ggtagctttt tgaaagccct gtataggctg ccacctcatc aaaattcaat actgtttttt
     2281 gtagtgataa aaggctttca atatttttta gcctctcatc aatcatctgt aaattatcca
     2341 tttttctttt ttattaaaat tgataaaatg gattagctaa tccttgttgt gaaatttcac
     2401 tacaaagaac ggcaagtatt gaaaataaag gtgtttacct gtggaagtgg taaaggtaaa
     2461 ggtaaaggct taaaagttac ttacaagctc atcaatagcc ttttgcttgt ctgatgatag
     2521 gtttatatca atattggttt ttaatgattt ccaaactgct ttaaaaggct ttccatcagg
     2581 cttgacaaag caggcagtca ttttctggta gtctgctttt ttgggattgt ggacaatatt
     2641 atttgttttt tgtaatctgt caataaaata aagcagctca ctggcattgt cctgatgcca
     2701 tttgattgga ttgatgcttt ctattgcctg ccctgtaaaa actgctttaa attgctcaca
     2761 ggtggtttct ggtgcaatta gcttgtagtt atcaatcatc aggctgtaaa gctctggaag
     2821 ctctttatct ggattgtttt gccatacata ggaagtcttt gtttgttgtt ttggtggctt
     2881 tctggtaggt tttttttgtt ggtagtctat ttcatttgat aaatactttt gcagccatct
     2941 gtcccaatca ttcaagccat ctgccccaat atctgcaagc agttcctttt ttctcaattc
     3001 ataaagctgc ttgtcaattt tagccactgc ctctgatggc tcaatcctgt ttagctcatc
     3061 agtccatttt tcaactgcat ttagtcttaa tttctggaag ttctttttaa attgctcaat
     3121 aaatctttgc agctgccatt ctgtttgtat gccataagtg gttttgtcat tctctgataa
     3181 tacaggctca tcataaaact gacaaaaact gataaaccat aaatagtttt ttcctttgtc
     3241 tgatagtggt atcttattga taaattctgt caatttctca aagtagccat caattctatt
     3301 ttgcattttt atatccatct gactaaatat ttatgtctgg tattttgttt actgcttccc
     3361 tttttttctc atcaactatc tttgcataaa tttgagtagt cttaagctct gaatgtccca
     3421 aaagtttgga aagtgtatat atttcagttc ctaaagtcaa ttgcaggact gcaaaagtat
     3481 gtctggcaca atggaaagtt atatcttttg tgatgcctgc cctcatcatc catttggtta
     3541 gctctacatt catatagctg ctatatttca agcctataaa aactctctca tctggattgc
     3601 cttgctcacc taaatagcct ctggcttgct ctgaaatatc taaatattga agtcctttag
     3661 tcttttgctg attgaatgtg atgcgccacc cttcattagt attttgaagc tctgaccatt
     3721 tcaggttatt aacatctgac caccttaagc ctgtcaagca ggaaaaaaga aaagcccttt
     3781 ttaatacatc atatctgcac tctgctttga caatagcctt tagttcatca attgtcaaat
     3841 attctctttt attctcaatt actctcacat tgaccacatc aatagcagga ttggaaagta
     3901 ttattttttt cctaactgct tcctttaggc aggctctgaa tttattgaag tatgatgata
     3961 ctgttgatga tgataaaggc tgtccgcttt tggttgtggc agtatttttt agatagtccc
     4021 tgaaattttc acaaaaagtt tggtctattt ctctgaatag tactttagtc cctgcaaatt
     4081 taatcaggtg ctttagtgtg ctgttccaat ttccataatt gccctttgac tgcttccttt
     4141 tttccatttg gtctgtgaag tagtctataa aattggtgct ttgcttaaac tcatttgtaa
     4201 agccatatag tccatttttg atttcaagct ctcttttggc tctgatgctt tctgcaagct
     4261 gcaaagtatc tttatttccc tgcttttcaa ttggtgtttt tggcttgtca atcaaataaa
     4321 ggtttaaata ttcatagtcc cttaagtttt tgactttgcc ctctggtgtt gtagtagtcc
     4381 ccttgtagat ttccaaaaat aaactgatgc tgcctgcttt ggattgcttt ctctgtctga
     4441 tatgtacttt cattttgctg ctattttgcg gttatttgcg gttattattt tgcaaagtta
     4501 caaattagca gcaaataaac aacaaataac agcaaaaaaa gtgaaaataa ataactatct
     4561 ttgtggctta aagtcaataa ataaaaggct ttagctaatc aaaggaaaac aactttactt
     4621 tccgatacaa aaagtgaagt aattttatta aagtcctgat attgaaagtc tttgcctgaa
     4681 tttatataga aaaaaaagta tattagcagc aaaaacagcc tatttattac ttttgagttg
     4741 caaagatagt tattttaaaa acatctttag tgatttctct aatcatttag cctctggttt
     4801 atatgtccca atcatcacac aaaccaccct caccaatctg gtaactttct ggctgtactt
     4861 ttggaagtgc aaaaggaagt aatttgacca aaagttccat tctgtcttta gggtctaatt
     4921 tattaaagtg ttctggaagc agctctattt cctgatgcaa tatgttttta agtactgccc
     4981 ttaattcttt tgtcaatttg tttggtgtgc ctgccactct gccaccatat tttttacctg
     5041 tattgttcgc cataatcaaa ccatttaaaa ctactttaga atattatact atttatttct
     5101 aattactttc taattaaatc cattatctgt aatgtctttt tgctgttctg attttcttgt
     5161 caagctctgc cactaaatct gatgatgctg tcatctctgg tgttttgcaa agcgctttta
     5221 tttcattttt taatctggaa taatactctg gcttgtcaaa ggctttctga tgccttaaat
     5281 tttcaatttc ctgcattatt ttatcctgac caatcatatt tattgccatc aggtttattt
     5341 gcttccaaaa gtctttagga ctattcatat ctgataaatt aaagtttatt gaatgtagct
     5401 tatctattgc ctcatattct gcaagccatt tgtcaaagat tgttatataa aatttttcat
     5461 ctgacaaggt gcttgctgtg atttctgcct gattaaattg ctttggaagt ctgcttataa
     5521 atctcatttc ataccttaaa acattctgac cattccaaac atctggcaaa ctcactcctt
     5581 taaccttgcc ctctgctatt ttgttgtaaa atagtttctg cctcactcca tttgaatagt
     5641 aaagactttt tggctgtggc tgcctgttat aatattggca ctcacctaaa aaaggatagt
     5701 aagcctctgg ctcaaagtcc atcaaaaaat tttgtgaaaa gtcaatccta tttactttag
     5761 ctatctgtat aggcaaataa agctcatctg acatcatttc aaaggctctg gcactatctg
     5821 accttgtcaa ggtgtgaaag ttatcaggca ggaagtattt tgcaaggctg ccctttaaag
     5881 acactccctg ctctgatata tttactttga aatttttcag atagccactt acatacattt
     5941 gaccatcatc tctggtgtgt tctgtaatgt ttgacaagtt ctgcaaggtt tttgataaat
     6001 caaaactgct tgccttgtct attgatagcc ataaatttat agtatcatac ataggctttt
     6061 gattaaattt gtcaattaaa acaacttact ataagacaag taaatcatat tgataaaaga
     6121 ttttggcttg tttccttaat tctctgacct catcatcaaa gccaccatct ataaattttt
     6181 gatagtatga tgatattaga tttctgccat caggaattat aagcagctca atttctgaat
     6241 aatccttatt tattaaaaat aagtagccat catttctaaa ttcaaataat ggattttgac
     6301 tgccattgct caaaagcctg cttgcatttg gatagccata tcctatttct ggatagtcta
     6361 aatcatcaat ataaatacta ctaaaattga taccattgcc tgtcaaactc cattctgcaa
     6421 gtcttttgct gtctgtattg acaaagtccc tgcaaggtgt tttataaaag aataactgcc
     6481 ctttgtgatt gacaaaattt gtcaagccct tgtaattgcc tgccaccata tcagtaaagg
     6541 aaatacaatc taatctggct ttgcttttaa ttttgtttgc agctctcacc tcatcaggaa
     6601 gcattacaaa tttatagtaa ttcattttgc acctcctttt cttaaggtta caaaagtagc
     6661 tgcctgattg tcaatctctt tggtagtggc tttcctattt tgcataagcc attggtctat
     6721 ttcttgttta ttgaaataga tatgctttcc ctgtggcttg tagcaaggta tgccaccgct
     6781 gcaagtcaat ttgtaaaggt agctttttga aagccctgta taggctgcca cctcatcaaa
     6841 attcaatact gttttttgta gtgataaaag gctttcaata ttttttagcc tctcatcaat
     6901 catctgtaaa ttatccattt ttctttttta ttaaaattga taaaatggat tagctaatcc
     6961 ttgttgtgaa atttcactac aaagaacggc aagtattgaa aataaaggtg tttacctgtg
     7021 gaagtggtaa aggtaaaggt aaaggcttaa aagttactta caagctcatc aatagccttt
     7081 tgcttgtctg atgataggtt tatatcaata ttggttttta atgatttcca aactgcttta
     7141 aaaggctttc catcaggctt gacaaagcag gcagtcattt tctggtagtc tgcttttttg
     7201 ggattgtgga caatattatt tgttttttgt aatctgtcaa taaaataaag cagctcactg
     7261 gcattgtcct gatgccattt gattggattg atgctttcta ttgcctgccc tgtaaaaact
     7321 gctttaaatt gctcacaggt ggtttctggt gcaattagct tgtagttatc aatcatcagg
     7381 ctgtaaagct ctggaagctc tttatctgga ttgttttgcc atacatagga agtctttgtt
     7441 tgttgttttg gtggctttct ggtaggtttt ttttgttggt agtctatttc atttgataaa
     7501 tacttttgca gccatctgtc ccaatcattc aagccatctg ccccaatatc tgcaagcagt
     7561 tccttttttc tcaattcata aagctgcttg tcaattttag ccactgcctc tgatggctca
     7621 atcctgttta gctcatcagt ccatttttca actgcattta gtcttaattt ctggaagttc
     7681 tttttaaatt gctcaataaa tctttgcagc tgccattctg tttgtatgcc ataagtggtt
     7741 ttgtcattct ctgataatac aggctcatca taaaactgac aaaaactgat aaaccataaa
     7801 tagttttttc ctttgtctga tagtggtatc ttattgataa attctgtcaa tttctcaaag
     7861 tagccatcaa ttctattttg catttttata tccatctgac taaatattta tgtctggtat
     7921 tttgtttact gcttcccttt ttttctcatc aactatcttt gcataaattt gagtagtctt
     7981 aagctctgaa tgtcccaaaa gtttggaaag tgtatatatt tcagttccta aagtcaattg
     8041 caggactgca aaagtatgtc tggcacaatg gaaagttata tcttttgtga tgcctgccct
     8101 catcatccat ttggttagct ctacattcat atagctgcta tatttcaagc ctataaaaac
     8161 tctctcatct ggattgcctt gctcacctaa atagcctctg gcttgctctg aaatatctaa
     8221 atattgaagt cctttagtct tttgctgatt gaatgtgatg cgccaccctt cattagtatt
     8281 ttgaagctct gaccatttca ggttattaac atctgaccac cttaagcctg tcaagcagga
     8341 aaaaagaaaa gcccttttta atacatcata tctgcactct gctttgacaa tagcctttag
     8401 ttcatcaatt gtcaaatatt ctcttttatt ctcaattact ctcacattga ccacatcaat
     8461 agcaggattg gaaagtatta tttttttcct aactgcttcc tttaggcagg ctctgaattt
     8521 attgaagtat gatgatactg ttgatgatga taaaggctgt ccgcttttgg ttgtggcagt
     8581 attttttaga tagtccctga aattttcaca aaaagtttgg tctatttctc tgaatagtac
     8641 tttagtccct gcaaatttaa tcaggtgctt tagtgtgctg ttccaatttc cataattgcc
     8701 ctttgactgc ttcctttttt ccatttggtc tgtgaagtag tctataaaat tggtgctttg
     8761 cttaaactca tttgtaaagc catatagtcc atttttgatt tcaagctctc ttttggctct
     8821 gatgctttct gcaagctgca aagtatcttt atttccctgc ttttcaattg gtgtttttgg
     8881 cttgtcaatc aaataaaggt ttaaatattc atagtccctt aagtttttga ctttgccctc
     8941 tggtgttgta gtagtcccct tgtagatttc caaaaataaa ctgatgctgc ctgctttgga
     9001 ttgctttctc tgtctgatat gtactttcat tttgctgcta ttttgcggtt atttgcggtt
     9061 attattttgc aaagttacaa attagcagca aataaacaac aaataacagc aaaaaaagtg
     9121 aaaataaata actatctttg tggcttaaag tcaataaata aaaggcttta gctaatcaaa
     9181 ggaaaacaac tttactttcc tatacaaaat ttagaaaaaa tagtactaag tacttcatct
     9241 gtagagattt
//
LOCUS       DAOPCH010000034         8855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_7415_length_8855_cov_7.811364, whole genome shotgun sequence.
ACCESSION   DAOPCH010000034 DAOPCH010000000
VERSION     DAOPCH010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7415_length_8855_cov_7.811364"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="PLU55_03250"
     CDS             <1..1288
                     /locus_tag="PLU55_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87103.1"
                     /translation="FTNSVTIPTNLSGNVSFYWTINFTNATGNYAFNTTLGYQQVENL
                     TIARCFTPTTEGLTLNFTLYDESSKNKINGSFESTFTYFVIGGSGTITQSYNYQSINE
                     NKSNWMFCINPSGYNLSFSGTASYYADYYDRREYIFSNATVGNFTQNIPLYLSLIADT
                     DVVTVTVQDRNYNPISGALVYIQEWNVGTNTYSTIGMFNTDSNGNGIINLELYNKWYR
                     AVVSIDGEIVKTTDVQKLADTTWFINVELATANPYELFDSISHGLTFNNETNITTFVW
                     TDGSGYIQSGCLYIYNNTNTRYSLIHSECVSSVAGIINYLLPGDGDYQVVGTIYLTPD
                     YNVSKVIDVLFVQIGVPELTRIVSPFGKILTFLFVGVATFLGVSVGSPIWGSMLLIGS
                     LFLTFKLGWIKLSSAILWGLLSVVIVIIFRQKRRVG"
     gene            1289..1741
                     /locus_tag="PLU55_03255"
     CDS             1289..1741
                     /locus_tag="PLU55_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87104.1"
                     /translation="MAEGNPTLTKILMASLVATFLIAITFGAYSEFIVLNNATIEDKY
                     SEAFNTIGAQYGNFGNLAKTTSDQGLVKNIFSFGKNVVSGTVNVFVVGLDAIGSFFSM
                     IPIISNVVESIALVIPGFQALLGLLTTLIVLYIGMRYIQSTSNKYELP"
     gene            1776..2090
                     /locus_tag="PLU55_03260"
     CDS             1776..2090
                     /locus_tag="PLU55_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87105.1"
                     /translation="MTRELLTFMNTSTSGEGMEGLINWVNVSSGNWFIPLFLVVFYIL
                     SIYVASKSEYKLGGWVLLLSLAFFILAMILQTVTQFNQMVIFAFGIGIIVGVVITFIE
                     NS"
     gene            2077..2271
                     /locus_tag="PLU55_03265"
     CDS             2077..2271
                     /locus_tag="PLU55_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87106.1"
                     /translation="MKTRKMINKKLNNKVKECQCCKCGHKWKQRGKHKPKKCSSCGNP
                     NWDKIDSKQMVKLIVGDFWK"
     gene            2436..2651
                     /locus_tag="PLU55_03270"
     CDS             2436..2651
                     /locus_tag="PLU55_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87107.1"
                     /translation="MVIGIYISDQIGDQLTDDSAAQNAAEAVVTALSTGTSWISILVV
                     VGFATIILTMLTSGLGSAAAEAGTPYY"
     gene            2850..3023
                     /locus_tag="PLU55_03275"
     CDS             2850..3023
                     /locus_tag="PLU55_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87108.1"
                     /translation="MKKLFFAHFKQNGMFGFEIKEDNFQGKLIASGKNFKSRKEAVKA
                     MNKTLKKSQRYLI"
     gene            3025..3378
                     /locus_tag="PLU55_03280"
     CDS             3025..3378
                     /locus_tag="PLU55_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87109.1"
                     /translation="MKQRKKKKLVKVLNKALTGAKGFYREGKKVFKEYSPGVVRFSER
                     ASTNIQRGFAVRRMPEDEEYLDLTQKTPAMVRRPENNRLKPDFSVKPQGRFPGMVDKR
                     RVRKRVDWDNLGVNF"
     gene            complement(3375..3809)
                     /locus_tag="PLU55_03285"
     CDS             complement(3375..3809)
                     /locus_tag="PLU55_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87110.1"
                     /translation="MFKKIPKEEWLKLSKEEQDYFTFEFNKSVEKRKRITLITTRTLA
                     LLCIFMLFWIGFVQFKAIENYNEIIDIYGNNGYCYLCGEKTLKACECQYFTNEFVLTN
                     QEIFNNYSKIVANYNGKKCSVMKVNENERYNQFLIPYDLSFD"
     gene            complement(3809..4048)
                     /locus_tag="PLU55_03290"
     CDS             complement(3809..4048)
                     /locus_tag="PLU55_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87111.1"
                     /translation="MFGKKKPVVHEVPKPLPIPKPQVIEAEEPMESYEEDEEESEQEE
                     TEAGLTEEMVVAYLKNYGERIQNLEAALFRMKGAI"
     gene            complement(4017..4577)
                     /locus_tag="PLU55_03295"
     CDS             complement(4017..4577)
                     /locus_tag="PLU55_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87112.1"
                     /translation="MIQAKFKIQDKWEYRDFKDKSELDTFLKENEGRFSDYKIKEDET
                     KNMFSKWWKSITHTKSNWKKVKASPYASLLLAYKARKIIIGLLIPYMLWMTYKMVVNY
                     QAVGFMNTVGRIITVVIMGFIIYKIWSTIPQAKKQIDYYKKYPHTINYCPTNTKETID
                     DIFSKIKQNKEKEVKQDVWKKETSSS"
     gene            complement(4579..4860)
                     /locus_tag="PLU55_03300"
     CDS             complement(4579..4860)
                     /locus_tag="PLU55_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87113.1"
                     /translation="MNIDITDLKSRDIIKILEMYPQGHLETKFERIFWCSDYLEPETR
                     YSHAGAPPNPGSPPPQPRKAIERMSDALGYMVQVPTREDRRRGYPGNIY"
     gene            complement(4857..5003)
                     /locus_tag="PLU55_03305"
     CDS             complement(4857..5003)
                     /locus_tag="PLU55_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87114.1"
                     /translation="MKLWLIIPGLIIFLFSFYPPFHLMSLVIGGVMIIIGLILKGKKK
                     EKRQ"
     gene            complement(5000..5128)
                     /locus_tag="PLU55_03310"
     CDS             complement(5000..5128)
                     /locus_tag="PLU55_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87115.1"
                     /translation="MKIYPDVIKEFESEHKITNIILLGEDGAIRNIEIIDFMEGEE"
     gene            complement(5125..5592)
                     /locus_tag="PLU55_03315"
     CDS             complement(5125..5592)
                     /locus_tag="PLU55_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87116.1"
                     /translation="MVEIEKPFRISLKLKEGKSPHVYLPQLKVLMEEDTSLHPMMEKT
                     LKLLKKTKTPYIMLSMLREVVIDSINFRHFDRSADPRGDWVCTLWSEDEIKQHQEDSE
                     GHIKAMLNMFEKLGYEFYDSKEVSEEYLRTLENLTKKQIEAQLQQREEMEEEE"
     gene            5690..5935
                     /locus_tag="PLU55_03320"
     CDS             5690..5935
                     /locus_tag="PLU55_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87117.1"
                     /translation="MKSNFSPNMKKVIQEMLGESKKEVVLMKEFEVRKIKRKPTKKKV
                     FVFKITKIKGDPFIEFFINNRFYTVFFREDGKWKEEN"
     gene            complement(5932..6522)
                     /locus_tag="PLU55_03325"
     CDS             complement(5932..6522)
                     /locus_tag="PLU55_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPJ87118.1"
                     /translation="MAQKRIDYISFEEFQKIYSACKDKKLKLCILLGFGSGLRISEII
                     GYKRKFKRKKNKETKQIELIQDDSEIPKLTADQILLREHQIRLEETKGKKWRITVTPP
                     SLTESMLKLLPINVPRRTIQHQFEQLTKKVLGKKMSFHTLRHGFGNYQANILNVPLPI
                     VQSMMGHSRLDTTGIYTKANPIQAIETSWKAMTENN"
     gene            complement(6604..7119)
                     /locus_tag="PLU55_03330"
     CDS             complement(6604..7119)
                     /locus_tag="PLU55_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87119.1"
                     /translation="MENLNKKTNQNTTQEINLDQEGSATHSGWMQFYAAYAKYRDECV
                     RLGKAVLGNDLNLAVMALSDYHSSLYTLAQQVFSFYNSDVEDVLTNEWLDISEKVNDF
                     LSKISDKDFRNQMQFEGQNSLDRDLKLLLLKYFNKVDRMAAEAGLQVGKESRGDKEPK
                     KGLLGFGVKRD"
     gene            complement(7244..8176)
                     /locus_tag="PLU55_03335"
     CDS             complement(7244..8176)
                     /locus_tag="PLU55_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87120.1"
                     /translation="MKKPPLVRYKGMPKKHSWCRWTAFRVKERNNSVNVRFVGDTGSG
                     KSWSALAFCEICAGMLNKKFTSERIYFSIKDVIDEVAQNEPPPGTIFFIDEQQVAASS
                     KRHQSKRAEAYAIFLSTIRSNRYIIVTTLPFTDMELKQVRRFFHVEIETHGADLNTKT
                     VRSTPRYQEYSRQKKDKIYSKRLIIEYVDDKTGIKRHRKLSYWDIPKPSQGLVDVYER
                     MKAQFKRNLYKKMSKELSEEGESGANPKIVAQETTLETLTDYQKAIYELMSGGEKMQK
                     VINQKLLERGFTSSPEKVSMNLKWMRKKGVIVIK"
     gene            complement(8173..8757)
                     /locus_tag="PLU55_03340"
     CDS             complement(8173..8757)
                     /locus_tag="PLU55_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87121.1"
                     /translation="MAYYNSQQPVTYPQLIIKQIEIIQGICSKELNDGEKVTKNLIGE
                     QTIHGEDTRHSFLQSVELLGSLLSPWFPKEDEKNENITSQFNYFCEVYDMELSEALQD
                     EEFKKLVCDSFNIKDTNKIIAQIKDNKLDTEINAFFLNIKIKEGRKIFRALVKLFKDH
                     DFLKEESFGDSGEDNGMTANDDEDESDVDGEFVE"
BASE COUNT         2916 a         1347 c         1253 g         3339 t
ORIGIN      
        1 tttcacaaat tctgttacaa taccaactaa tttaagtgga aatgtatctt tctattggac
       61 aatcaatttt acaaatgcaa ctggaaatta cgcattcaat acaactttgg gatatcagca
      121 agtggaaaat cttacaatag caagatgttt tactcctaca actgaaggtt tgactttaaa
      181 ttttacatta tatgatgaat caagtaagaa taaaattaat ggttcttttg aatcaacatt
      241 tacctatttt gtgattgggg gatctggaac aataactcaa agttataatt atcaatcaat
      301 aaatgaaaat aaatccaatt ggatgttttg tataaatcca agtggatata atttatcttt
      361 tagtggaact gcaagttatt atgcagatta ttatgataga agggaatata ttttttcaaa
      421 tgcaacagta ggtaatttta ctcaaaacat ccctctttat ttatcgttaa tagcggatac
      481 agacgttgta actgtaacag ttcaagaccg aaattataat cctatttcag gagcattagt
      541 ttatattcag gaatggaatg ttgggacaaa tacttattct acaattggaa tgtttaatac
      601 agattcaaat ggtaatggaa taataaatct tgaattgtat aataaatggt atagagcagt
      661 tgtttctata gacggagaaa tagttaaaac tacagatgtt caaaaattag ctgatacaac
      721 ttggtttata aatgttgaat tagcaactgc aaatccttat gaattatttg atagcattag
      781 tcacggatta acttttaata atgaaacaaa tataactact tttgtatgga ctgatggttc
      841 aggatatatt caaagtggat gtttgtatat ttataataat acaaatacaa gatattctct
      901 tatacattcg gaatgtgttt caagtgttgc tggaataata aattatttat tacctggaga
      961 tggagattat caagtagttg gaacaattta tctaactcct gattataatg tttcaaaagt
     1021 aatagatgtc ttatttgttc agataggagt tccagaatta acaagaatag tatctccttt
     1081 tggtaagata ctaacatttt tattcgttgg tgttgctacg ttcttgggag tatcagtagg
     1141 aagcccaatt tggggttcaa tgcttttaat tggttcttta ttcttaacat tcaaattagg
     1201 atggataaag ttatctagtg ctatattgtg gggactactt tcagtagtta tagtaattat
     1261 attcagacaa aaaaggagag taggataaat ggcagaagga aatccaacat taactaaaat
     1321 attgatggca tcattagttg caacattttt aattgctata acctttggtg cttattcaga
     1381 gtttatagta cttaacaacg ctacaataga agataagtat tcagaagcat ttaatacaat
     1441 tggtgcacaa tatggtaatt ttggaaatct tgcaaaaaca acttcagatc aaggattagt
     1501 taaaaatata ttcagctttg gtaaaaatgt agtatctggt acagttaatg tatttgttgt
     1561 aggattagat gctataggaa gtttcttttc aatgatacct ataataagta atgttgtaga
     1621 atctatagca ttagttatac ctggtttcca ggctttatta ggattattaa caacattaat
     1681 agtattgtac attggaatga gatacataca atcaacttca aataaatatg aattgcctta
     1741 aaaaaaatta aataatatag aaaacaaaaa ataaaatgac aagagaatta ttaacattta
     1801 tgaatacttc aacaagtgga gaaggaatgg agggtttaat aaattgggta aatgtatctt
     1861 ctggaaattg gtttatacct ttgtttttag ttgtatttta cattttatct atttatgttg
     1921 cttcaaagtc tgaatataaa ttagggggat gggttctttt acttagtctt gcttttttca
     1981 tattagcaat gatattacaa actgttactc agtttaatca aatggtaata tttgcatttg
     2041 gaataggcat aatcgtcggt gtagtaataa cttttattga aaactcgtaa aatgataaat
     2101 aaaaaattaa ataataaagt aaaagaatgt caatgttgta aatgtgggca taaatggaag
     2161 caaagaggaa aacacaaacc aaaaaaatgt tcttcttgtg gaaatcccaa ttgggataaa
     2221 atagattcta aacagatggt taaactaatt gttggagatt tctggaaata gatatttaat
     2281 tattagatgt tggaaaatct atgttggaaa aatcatttaa aattgtttat ataatactct
     2341 tttttaactt ttttaactaa actaaaacaa accaaaaaat ggcatcattt aatctacagt
     2401 cattcatcat ctctgtagta attgttggtg taacattggt cattggtatt tacatatctg
     2461 atcagattgg tgatcagtta acagatgatt ctgctgcaca aaatgctgca gaagcagttg
     2521 ttactgcact ttctacaggt acatcttgga tatcaatcct ggttgttgta ggattcgcta
     2581 caataatctt aacaatgttg acatctggtc taggtagtgc tgctgcagaa gcaggaacac
     2641 catactacta aacctaacag ttactttgta gagattatgt agaagttgtg ttgcccctac
     2701 agctaaaatc aaaggggaac tggagtaatc tgggaagcca ccaaaattaa ataacaataa
     2761 aatgactatg gttatggatg aaccaaaatt acttaataag aaagggttaa aaaaaaggag
     2821 atcttggaaa aggagaggat tattgtaaga tgaaaaaact attttttgca cactttaaac
     2881 aaaatggaat gtttggattt gaaataaaag aagataattt ccaaggtaaa ttaattgctt
     2941 ctggtaaaaa ttttaaaagt agaaaagaag ctgttaaggc tatgaataaa actttaaaaa
     3001 aatcacagag gtatttgata taaaatgaaa caaagaaaaa agaaaaaatt agtgaaggtt
     3061 ttaaataaag cattaactgg agcaaaagga ttctatagag aaggcaaaaa agtgtttaaa
     3121 gaatatagcc ctggagttgt gaggttcagt gaaagagctt ctacaaacat tcagagaggc
     3181 tttgcagtga gaagaatgcc tgaagatgaa gaataccttg atttaacgca aaaaacccct
     3241 gcaatggtta gaagacctga aaataataga ttaaaacctg acttttctgt taaacctcag
     3301 ggaagatttc caggaatggt tgataaaaga agggttagaa aaagagttga ttgggataat
     3361 ttaggagtta atttctaatc aaaactcaaa tcgtaaggaa ttaaaaactg attatatctt
     3421 tcgttttcat taactttcat tacagaacat ttctttccat tgtaatttgc tacaattttt
     3481 gagtaattat taaatatctc ttggtttgta agaacaaatt catttgtaaa atattgacat
     3541 tcacaagctt tcaatgtttt ttctccacag agataacaat atccattatt tccatagata
     3601 tctattatct cattataatt ctcaattgct ttgaattgaa caaaacctat ccagaataac
     3661 ataaatatac ataaaagggc taatgttcta gttgtaataa gtgttattct ctttctcttt
     3721 tcaacacttt tattaaattc aaaagtaaaa taatcttgtt cttccttact taacttgagc
     3781 cattcctctt taggaatttt cttaaacatt atatagcccc cttcattctg aataatgctg
     3841 cttctaaatt ctgtattctt tctccatagt tcttaaggta tgcaacaacc atttcttcag
     3901 ttaaacctgc ttctgtttct tcttgttctg attcttcttc atcttcttca taagattcca
     3961 ttggttcttc agcttcaatt acctgaggtt taggtattgg aagtggcttt ggtacttcat
     4021 gaactactgg tttctttttt ccaaacatct tgttttacct ccttctcttt gttttgtttt
     4081 atcttagaaa aaatatcatc tatagtttct tttgtatttg taggacaata gttaattgta
     4141 tgaggatatt tcttataata atctatctgt tttttagctt gaggtattgt agaccaaatt
     4201 ttataaataa taaatcccat tattactact gttattattc ttccaacagt attcataaat
     4261 cctactgctt gataatttac aaccatctta taagtcatcc ataacatata tgggattaac
     4321 aatccaatta taatctttct agccttgtaa gctaataaaa gactagcata aggagaagct
     4381 tttacctttt tccaattact tttagtgtgt gttatactct tccaccattt agaaaacata
     4441 ttttttgttt catcttcttt tatcttataa tcagaaaatc ttccttcatt ttcttttaga
     4501 aaagtatcta attcgctttt atctttgaaa tctctatatt cccatttatc ttgtatcttg
     4561 aattttgctt gtatcatttt aataaatatt tccaggataa cctcttcttc tatcttctct
     4621 tgttggaact tgtaccatat atcccaatgc atcactcata cgttctatgg cttttctagg
     4681 ttgaggagga gggctcccgg ggttaggggg agctccagca tggctatacc tggtttcagg
     4741 ctcaagatag tcagagcacc aaaatattct ctcaaattta gtttccaggt gaccttgagg
     4801 atacatttca agtatcttaa taatatctct tgattttaaa tcagttatat ctatattcat
     4861 tgtcttttct ccttcttttt tcctttaagt attaatccta ttataatcat aactcctcca
     4921 ataactaaac tcattaaatg aaatggagga taaaaagaaa ataaaaagat aattaatcct
     4981 ggaatgatta accaaagttt cattcttctc cctccataaa atcaattatt tctatatttc
     5041 taattgcacc atcttccccc aagagtatta tgtttgttat tttatgttcg ctttcaaatt
     5101 cttttataac atctggatat atcttcattc ttcttcctcc atttcttctc tttgttgtaa
     5161 ttgtgcttct atttgttttt tagttaaatt ttcaagtgtc ctaagatatt cttcacttac
     5221 ttctttggaa tcataaaatt cataacccaa cttctcaaac atatttagca ttgctttaat
     5281 atgcccttct gaatcctcct gatgttgttt tatttcatct tcactccata gtgtacacac
     5341 ccaatctcca cgaggatctg cacttctgtc aaagtgtctg aagttaatag aatctataac
     5401 aacctctctt agcatagata acataatgta aggggttttt gtttttttaa gaagttttaa
     5461 tgttttctcc atcattggat gtaaactagt atcttcttcc attagtactt ttaattgagg
     5521 aagatataca tgaggagatt ttccttcttt aagctttaaa gaaattctaa aaggcttttc
     5581 tatttccacc attactttta caagattttc ttatttttaa atatagcgaa tatagcgaat
     5641 aaaactcaat caccattata tttaaaaaaa catatcactt ataaaaaata tgaagagtaa
     5701 ctttagtcct aacatgaaaa aggttattca agaaatgtta ggagaatcaa aaaaagaagt
     5761 ggtgttaatg aaagagtttg aagttagaaa aataaaaagg aaaccaacta aaaagaaagt
     5821 ttttgttttt aagataacaa aaattaaagg agatcctttt attgagttct tcataaataa
     5881 tagattttac actgttttct ttagagaaga tggaaagtgg aaagaagaga attaattatt
     5941 ttctgtcatt gctttccagg aggtttctat tgcttgtata ggatttgctt tggtgtatat
     6001 cccagtagta tctaatcttg aatgtcccat cattgattga actatcggta atggaacatt
     6061 aaggatattt gcttgatagt ttccaaaacc atgtcttaaa gtatgaaagc tcattttctt
     6121 accaagaacc ttctttgtaa gttgttcaaa ctgatgctgt attgttcttc taggaacatt
     6181 aataggaagc aattttaaca tactctctgt taatgatggg ggagtaacag ttattctcca
     6241 ttttttacct ttagtttctt ctaatcttat ctgatgctct cttaaaagaa tctggtctgc
     6301 agttaattta ggtatttcag aatcatcttg aattagttct atttgctttg tttccttatt
     6361 cttctttctc ttgaattttc tcttgtaacc aattatctca cttattctaa gtccagaacc
     6421 aaatcccaaa aggatacaca acttcaattt tttatcttta caagccgagt aaattttttg
     6481 aaattcctca aacgaaatgt aatcaatcct cttttgagcc attttttatg caatttttta
     6541 tttttgttct cctatgtata ttttttaatt ttacacaatt ttttagaatt gtgcaatgtt
     6601 attttaatct cttttaactc caaaacctaa taaacctttc ttaggttctt tatctcctct
     6661 tgattctttt ccaacttgaa gtccagcttc agcagccatc ctatctactt tattaaaata
     6721 ctttaataac aataatttca aatctctgtc taaagaattt tgtccttcaa attgcatttg
     6781 atttctaaaa tctttgtcac taatctttga taaaaaatca ttaacttttt cactaatgtc
     6841 taaccactca ttagttaaaa catcttcaac atctgaatta taaaaactaa atacctgttg
     6901 agctagagta tataaagaag agtgataatc tgaaagagcc attactgcaa gattcaaatc
     6961 atttcctaaa actgcttttc caagtcttac acattcatct ctatactttg cataagcagc
     7021 atagaactgc atccaaccac tgtgagttgc agatccttct tggtctaaat taatttcttg
     7081 tgttgtgttt tgatttgttt ttttatttaa gttttccatt ttttaattgt gggtttactg
     7141 ttgaatgaca cataatttgg tacatctata cattaattta aatatatata ttcccatata
     7201 tataatggca ttatataata aattgcacta tttttattat atttcatttt ataactatta
     7261 ctcctttttt tctcatccat tttaagttca ttgaaacctt ttctgggctt gaagtgaatc
     7321 ctctttctaa taatttctga ttaattacct tttgcatttt ctctccacca ctcattaatt
     7381 cataaatagc tttctgataa tctgttaaag tttcaagagt agtttcttgt gcaactatct
     7441 ttggattagc accactttct ccttcttcag aaagttcttt agacattttt ttataaagat
     7501 ttcttttaaa ctgggctttc attctttcat aaacatcaac taatccttga cttggtttag
     7561 ggatgtccca ataggataat tttctatgtc ttttaattcc agttttatca tcaacatatt
     7621 ctattattaa cctcttagaa tatattttat ctttcttttg tctagaatat tcctggtatc
     7681 ttggtgtgga acgaacagtt ttagtattta agtcagctcc atgtgtttct atttcaacat
     7741 gaaaaaatct tcttacttgc tttaattcca tatctgtaaa tggtaatgtt gttacaataa
     7801 tatatctatt agaccttatt gtggataaaa atattgcata agcttctgct cttttagatt
     7861 gatgtctttt cgaactagct gctacttgct gttcatctat aaaaaatata gttcctggtg
     7921 gaggctcgtt ttgtgcaact tcatctataa catctttgat tgaaaaatat attctttcag
     7981 aagtaaattt tttatttaac ataccagcac atatttcaca aaaagctaaa gcgctccaac
     8041 tcttcccact tccagtatcc ccaacaaacc taacatttac agaattattt ctttctttaa
     8101 ctctgaaagc agtccatcta caccaactat gtttttttgg cattccttta tatctaacta
     8161 gtggtggttt tttcattcta caaattctcc atcaacatct gattcatctt catcatcatt
     8221 agcagtcata ccgttatctt ctccactatc cccaaaactc tcttctttca agaagtcgtg
     8281 gtccttgaat aatttaacta atgcacgaaa tatttttcta ccttctttta tttttatatt
     8341 caagaaaaaa gcattaattt ctgtatctaa tttattatct ttaatttgtg ctattatttt
     8401 atttgtatct tttatgttaa aagaatcaca aactaatttt ttaaattctt catcttgtaa
     8461 tgcttcagaa agttccatat cataaacttc acaaaaataa ttaaactgag aagtgatatt
     8521 ctcatttttt tcatcttctt taggaaacca aggagaaagt aatgaaccta ataattctac
     8581 actttgaaga aaactatgtc ttgtatcttc tccgtggatt gtttgttctc caattaaatt
     8641 tttagttact ttttccccat catttaactc ctttgaacaa ataccttgaa taatctcaat
     8701 ttgtttaatt attaactgag ggtaagttac tggttgttga gaattatagt atgccatttt
     8761 atatattaat tataataatt atgcaatact tttaaatata gcgatagagc gaggggagga
     8821 attgaacctg ccatccttct cctcatgagg gagat
//
LOCUS       DAOPCH010000035         8707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_7571_length_8707_cov_4.610379, whole genome shotgun sequence.
ACCESSION   DAOPCH010000035 DAOPCH010000000
VERSION     DAOPCH010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 8707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8707
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7571_length_8707_cov_4.610379"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PLU55_03345"
     CDS             <1..353
                     /locus_tag="PLU55_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPJ87122.1"
                     /translation="TSWVKEFKPSDYNKKLKKAKKETVKAKRILNNFKSLDILVCHQP
                     PYGYLDKVSPKYNPPKDWIGKHAGSKVILDYIKKYQPKYVFCGHIHEGEGKAKIGKTE
                     VYNLGVAGHKIINL"
     gene            405..1676
                     /gene="aspS"
                     /locus_tag="PLU55_03350"
     CDS             405..1676
                     /gene="aspS"
                     /locus_tag="PLU55_03350"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HPJ87123.1"
                     /translation="METSYVKELKVGKVVLLKGWVYEVRVLAKLGFVLLRDKTGIVQC
                     VVQGKELEKLSNLSLESAVEIKGKTKSAKVKAEFTRKDIEVDVESLEIVSPAEKLPIH
                     VNEKTTTTEFQNRLDYRFLDIRKPKIQAIFEIQTVIANSFREHFCNKDFIEIQPPCII
                     SAASEGGTELFSVQYFEQKAYLAQSPQLYKQMLACSLGKTFTITPVWRAEKHNTTRHI
                     NEIRQMDIECAFTNQMSIMKELEEVVQFIIKQVLEKCKNQLEILGIKHLKVPKGVYLS
                     YEEAIKKVGGKIGEDFTPEEERKLCEMYPGDIVFTHSWPTSIKPFYIMTKDEKKDAKL
                     SEGFDALYGGIEISSGGQRIHIPDLLIARLKTKGLNPNNFKDYIDSFRFGAPPHSGWS
                     IGLERLTQIICGLDNVKEATMFPRDRTRLTP"
     gene            complement(1683..2825)
                     /gene="tgt"
                     /locus_tag="PLU55_03355"
     CDS             complement(1683..2825)
                     /gene="tgt"
                     /locus_tag="PLU55_03355"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPJ87124.1"
                     /translation="MVNKDTFTIIHKDKKTSARIGILQTKKGKVETPFFMPVATRGSV
                     KTLTSEELQEMKVPAVISNALILSLREGIIITDKFKGIGKFMNYKGINFTDSGGFQMY
                     SPSIYLKSTKEGVIFRNPLNGEKILMTPEKNMELQLKISSEVAMCLDSMPMYHNTTKT
                     QIEDSVNKTTIWAKRCKVHHDTLQKNLPKSKRQLLFGIIQGGKYEDLRETSTKQLLKL
                     DFDGYSIGGFGLGETIQEEFNIVKQVKSLLLENKPVYLMGIGTPIEILKGIELGVDIF
                     DSRMPTQNARRGTLFTSEGKLRILRENCKFSPEPIDKNCKCFVCKNYSRAFIRYQLIH
                     DELTGKKLATYHNIYYLTKLIETAKEKIKQGTFQSFLKEVEEKYNE"
     gene            2906..4258
                     /gene="asnS"
                     /locus_tag="PLU55_03360"
     CDS             2906..4258
                     /gene="asnS"
                     /locus_tag="PLU55_03360"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012186921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPJ87125.1"
                     /translation="MKTHTQKFISIQEAIEKARHSQVRSGSSKDQAKGEVAVRGWVYR
                     ERGSNAMKFIVLRDSSNIIQCVLERKNFEKQWDEIDKIKIESSLEIIGEIKEDKRAPT
                     GFEIQVKKINIIQIAEDFPINKDLNEELLGDRRHLWLRSRKMTAVLKIRSTVFNAIHE
                     YFRNNGFYEYQSPIFQAVQCEGGSILFNVDYFGKKGVFLAQTWQLYAEPAIFSLEKIY
                     TLAPSFRAEESKTSRHLTEYWHAEMEVAWATFKDIQDYGEGLIKNIVKRVLNENKPEL
                     EILERDIKKLEPTVKKPFPRITYDDALKILKDKCKVKIPWGKDLRTIEEDELSKLYDT
                     PIIVTNYPKEVKAFYMKGTEGNPKVVNGCDFIGPEGYGELIGGSEREADPDKIKKRLI
                     EMGEKPEQYSFYLDTRKYGSVPHGGFGMGVERVVSWICGLDTIKDAIAFPRTMTRFTP
                     "
     gene            complement(4287..4673)
                     /locus_tag="PLU55_03365"
     CDS             complement(4287..4673)
                     /locus_tag="PLU55_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87126.1"
                     /translation="MGNYYVDALRGREKKTETILSPKRQIVKYQKEKCYMCEKNLGTA
                     MCYFAEIEGPDMNTGINGKSTRAICSSCFFSLGKNPVKQARKSRDTEKEKTKAKEKQQ
                     EEEEVNLFKELRLKHNTKEEMYSQWD"
     gene            5016..5681
                     /locus_tag="PLU55_03370"
     CDS             5016..5681
                     /locus_tag="PLU55_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPJ87127.1"
                     /translation="MKVGDVELKWLGHSGFFIKNSRIIYIDPYNIKEGLEKADIILIT
                     HSHYDHCSVADLKKIVQDGTRIILTADSQSKITRFDVPVKIDIVEPGKELEIGEIKIS
                     AFPAYNLDKHFHLKDEGWVGYLLKLNNLIIYHAGDTDLIPEMQKLTGYKQPGKEFVAL
                     LPIGGRFTMNVEEAVEAAKLIKPSIAIPMHFGSIVGVNEDANEFKDLCEENNIKCEIL
                     EKE"
     gene            5764..6486
                     /locus_tag="PLU55_03375"
     CDS             5764..6486
                     /locus_tag="PLU55_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87128.1"
                     /translation="MKESVIKKVYIKKLTKISGFKVWIVNGKYIRGNIDREFTNYGQY
                     YRFRFIPKDEFWIDKENNPGEARYFIDTMFIMNRLLSKGMDYKSARKIANRIEKRERS
                     KSKLIQKHIGIKKNKGNIFKSVYKELIKEYSKGKIKVWIVNGELVRGLFFLDFTEGGH
                     DKVYSFIPSNEIWLDDDLDPSEIKFVLLHEVHERNLMSKGMGYEKAHKSSSEIEYYCR
                     KHPEKLDKYLMKEFKKAKKIKE"
     gene            6488..7054
                     /locus_tag="PLU55_03380"
     CDS             6488..7054
                     /locus_tag="PLU55_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing 3 family protein"
                     /protein_id="HPJ87129.1"
                     /translation="MPRDRFVQRKNAVLSKIDRSSAGEWDDKIKKLCEKINKTSDFYT
                     TSSCSGRVILMISKDKKGKDLFLKIWHEKVYFDDFKEILNSLLNKNDIIKFKLEPPIL
                     HIACRDLKKATYILEKAKFLGFKRSSILTFDRNIVVEINSSERFEFPIIKNNKILVDD
                     EFLKLVLSMSNYKMERGWEKIKRLEKAI"
     gene            7227..8309
                     /gene="ftsZ"
                     /locus_tag="PLU55_03385"
     CDS             7227..8309
                     /gene="ftsZ"
                     /locus_tag="PLU55_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPJ87130.1"
                     /translation="MVLDFAKEAIANTATHSINFDELKAGKANIKVIGCGGMGSNAVT
                     WLFNKGINGATIYACNTDALHLSVTKADEKILIGKELTRGLGAGGKPKIGREAAKEAL
                     SELKRAVGSADMVFVLAGMGGGTGTGSAPVVSQIAKESGAVVIGVITMPFESEKARID
                     KAEFGLQELREVTDTCIVLDNNRLVDIAGQLPIEQAFAVANELVSTMIKGIVETITLP
                     SLINLDYADVSAIMKNGGVSVIGIGESDSQDKVNEAVRQALTHPLLDVDYRGASGALI
                     HITCGPDLKLEEFDLIGRTVSENLGTEAQVIIGARISKDFSGRVRVITIMTGVKSPYV
                     LGKDIEEHYSSSPSREISDLGIEVWR"
     gene            8438..>8707
                     /locus_tag="PLU55_03390"
     CDS             8438..>8707
                     /locus_tag="PLU55_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87131.1"
                     /translation="MGKNLIENLSKILELDSCKASICAIKGITVINDNNFVLEKRGLI
                     RDYRKTTGDYRTGWFKSLFTGKLELTGKQIENYQYEYSKDIRNYNF"
BASE COUNT         3075 a          963 c         1638 g         3031 t
ORIGIN      
        1 atacaagttg ggttaaagag tttaaacctt ctgattataa taaaaaatta aagaaagcaa
       61 aaaaagaaac agttaaggca aagagaattt tgaataattt taaatcttta gatattttag
      121 tctgtcatca gcctccttat ggttatttag ataaagtttc tccaaaatat aatccaccta
      181 aagattggat tgggaaacat gcaggaagta aagttatctt agattatatt aaaaaatacc
      241 aacctaaata tgttttttgc gggcatatcc atgaaggtga aggtaaagcc aaaattggta
      301 aaacagaagt ttataatttg ggtgttgcag gacataagat aattaattta tagatttttt
      361 atactagata aattaaaaaa cccctttctt cttttatttt aatcatggaa acaagttatg
      421 ttaaagaatt aaaagtagga aaagtagttt tgttgaaagg ttgggtatat gaagtaagag
      481 ttcttgctaa attaggtttt gttttactta gagataaaac aggtattgtt caatgtgttg
      541 ttcaaggcaa agaattagag aaattatcaa atttgagtct tgagagtgca gttgaaatta
      601 aaggaaagac aaaatctgcg aaagttaaag cagaatttac aagaaaagat attgaagttg
      661 atgttgaaag tttagagatt gtaagtcctg cagaaaagtt gcctattcat gttaatgaga
      721 aaacaactac aacagaattc cagaataggt tagattatag atttttagat ataagaaaac
      781 caaagattca ggctattttt gaaatacaga cagttatagc taattcattc agagagcatt
      841 tttgcaataa agattttatt gagatacagc ctccttgtat tataagtgca gcttcagaag
      901 gaggaacaga attattttct gtgcagtatt ttgagcagaa agcatattta gctcaatctc
      961 ctcaattgta taaacaaatg ttggcttgtt ctttaggaaa aacatttaca ataactcctg
     1021 tttggagagc agagaaacat aatactacta gacatataaa tgaaataaga cagatggata
     1081 ttgaatgtgc atttacaaac cagatgtcta ttatgaaaga gcttgaagaa gttgtgcagt
     1141 ttataattaa acaagtctta gaaaaatgta agaaccaact tgagattctt ggaataaaac
     1201 atttgaaagt tccaaaagga gtttatttga gttatgaaga agcaattaag aaagttggtg
     1261 gaaagattgg agaggatttt actccagaag aagaaagaaa actttgcgag atgtatcctg
     1321 gagatatagt ttttacacac tcgtggccta cttcaattaa accattttat attatgacta
     1381 aagatgaaaa gaaagatgct aaattaagtg agggctttga tgctctttat gggggaattg
     1441 aaatttcttc tggaggtcag agaatacata taccagattt actgatagca agattgaaaa
     1501 ctaaaggact taatccaaat aattttaagg attatattga tagttttaga tttggagctc
     1561 ctccacattc tggatggtca attggattag agagattaac tcagattata tgtggattgg
     1621 ataatgttaa ggaagctaca atgtttccaa gagatagaac tagattaact ccttaatttt
     1681 atttattcgt tatacttttc ttcaacttct tttaaaaagc tttgaaatgt tccttgctta
     1741 attttttctt ttgcagtttc tataagtttt gttaaatagt aaatattgtg gtaagttgca
     1801 agtttttttc ctgtaagttc atcgtgtatt aattggtaac ggatgaaggc tctgctatag
     1861 tttttacata caaaacattt gcagtttttg tctattggtt caggactgaa tttacaattt
     1921 tctcttaaga tacgtagttt tccttctgat gtgaaaagag ttcctctgcg ggcattttgt
     1981 gttggcattc ttgaatcaaa aatatcaact cctaattcta ttccttttaa tatctcaata
     2041 ggagttccaa tacccattaa ataaactggt ttgttttcta gaagaagaga ttttacttgt
     2101 tttacaatat taaattcttc ttgaattgtt tcacctaaac caaatcctcc aatagaatat
     2161 ccatcaaaat ctaattttag caattgtttt gttgaagttt ctctaaggtc ttcatatttc
     2221 cctccttgaa ttattccaaa gagaagttga cgtttagatt ttggtaagtt tttttgtagt
     2281 gtgtcgtggt ggactttaca tctttttgcc catattgttg ttttatttac agagtcttca
     2341 atttgtgttt ttgtagtgtt atggtacata ggcatagaat ctaaacacat tgcaacttca
     2401 cttgatattt ttagttgtaa ttccatattt ttttcaggag tcattaatat tttttcacca
     2461 tttagaggat ttctgaagat aactccttct tttgttgatt ttaaataaat gcttggagaa
     2521 tacatctgaa atcctccaga atctgtaaaa tttattcctt tgtagttcat gaattttcca
     2581 attcccttga atttgtctgt gattattata ccttctctta aagagagtat tagtgcatta
     2641 gatataactg caggaacttt catttcttgg agttcttctg aagtgagagt tttaacactt
     2701 ccacgagttg caacaggcat aaaaaaagga gtttcaactt ttcctttttt tgtttgaaga
     2761 attcctattc ttgcagaggt ttttttgtct ttgtgaatta ttgtgaatgt gtctttattt
     2821 accattaaat tcattaaaaa ttcttgtata taaagattag taatagtaga taaatttatt
     2881 aaaccatttt ttcttaagtt taattatgaa aacacatact caaaaattta tttcaattca
     2941 agaggcaatt gaaaaggcga gacactcaca agttaggtct ggctcgtcca aagaccaagc
     3001 caaaggagaa gtagcagtgc gtggatgggt ttatagagaa agagggagca atgctatgaa
     3061 atttattgta ttaagagata gttcaaatat aatccagtgt gttttagaaa gaaaaaattt
     3121 tgaaaaacaa tgggatgaaa ttgataaaat taagattgaa agttctttag agataattgg
     3181 agagataaaa gaagataaaa gagctcctac tggatttgag attcaggtta aaaaaataaa
     3241 tataattcaa attgcagagg atttcccaat aaataaagat ttgaatgaag aattgcttgg
     3301 agatagaaga cacttatggt taagaagcag aaagatgact gctgttttga aaattagaag
     3361 tacagtattt aatgcaattc atgaatattt tagaaataat ggattttatg aatatcaaag
     3421 tcctattttt caggcagttc agtgtgaagg tggaagcatt ttgtttaatg tggattattt
     3481 tggaaaaaaa ggagtttttt tagcacaaac ttggcaattg tatgctgaac ctgctatttt
     3541 ttctcttgaa aaaatttata ctttagctcc ttcttttaga gccgaggaaa gtaaaacttc
     3601 aagacattta actgaatatt ggcatgctga gatggaagtt gcatgggcta cattcaaaga
     3661 catacaagac tatggtgaag gactaataaa aaatattgtt aaaagagttt taaatgaaaa
     3721 taaaccagaa ttagaaattt tagaaagaga tattaaaaaa ttagaaccga ctgtaaaaaa
     3781 accttttcca agaataactt atgatgatgc tttgaaaatt ttgaaagata aatgtaaggt
     3841 taaaattcct tggggaaaag atttgagaac aattgaagaa gatgaattga gtaagttgta
     3901 tgatactcct ataattgtta caaattatcc taaggaagtt aaggcatttt atatgaaggg
     3961 aacagaagga aatcctaaag ttgttaatgg atgtgatttt attgggcctg aaggatatgg
     4021 agaattaatt gggggaagtg agagagaagc tgaccctgat aaaattaaga aaagattgat
     4081 tgagatggga gaaaaaccag aacaatattc attttatttg gatacgagaa aatacggaag
     4141 tgttcctcac ggaggatttg gtatgggtgt tgagagagtt gtatcttgga tatgtggatt
     4201 agatactata aaggatgcaa ttgcatttcc aagaacaatg acgagattta ccccttaaat
     4261 ttctaattaa ttttatagaa ttatatttaa tcccactgag aatacatttc ttcttttgtg
     4321 ttgtgtttaa gtcttaattc tttgaataaa ttaacttctt cttcctcttg ttgtttttct
     4381 tttgcttttg ttttttcttt ttctgtgtct cttgattttc ttgcttgctt tactggattt
     4441 tttccaagag aaaagaaaca tgaagaacaa attgctcttg ttgattttcc gtttatgcct
     4501 gtgttcatat ctgggccttc aatctctgca aaataacaca ttgcagttcc aagatttttt
     4561 tcacacatat agcatttttc tttttgatat tttacaattt gtctttttgg agaaagaatt
     4621 gtttctgtct ttttttcacg tcctcttagc gcatcaacat aataatttcc cattttgata
     4681 atttatccat agaatatgct tttttaagct tttccactta tttttatcta tttttgagtg
     4741 tatcaataga tttaaaaata ttgaagaaaa gtagatgata tttaaatttg gaggtataac
     4801 aatgcctaga tatgatgaag aggaaatgcc tgaagatgaa gacgatgaag aatttgatga
     4861 cgaagattct gatggcgaat acttcgatga tgatatgtaa gttctaatag ttttcatggg
     4921 gcttctgtat ctttatattt aaattagata gaagttccct cattttctta atttttacaa
     4981 taatctttaa aaaacatgtt tctttgttta ctttaatgaa ggtaggagat gttgaattaa
     5041 agtggttggg tcattctgga ttttttataa aaaattcaag aataatctat atagacccgt
     5101 ataatataaa agaaggtttg gaaaaagcag atataatctt aattactcat agtcattatg
     5161 atcattgtag tgtagctgac cttaagaaga tagttcaaga tggaacgaga ataatcttga
     5221 cagcagattc tcagagtaaa ataactagat ttgatgtccc tgttaaaatt gatattgttg
     5281 aacctggaaa agaattagag attggggaga ttaagatttc agctttccca gcttataatt
     5341 tagataaaca ttttcattta aaggacgaag gatgggtggg ttatttgctt aaacttaata
     5401 atttgattat ttatcatgca ggagatactg atttaattcc tgagatgcaa aaactcactg
     5461 gatataaaca acctggaaaa gaatttgttg cacttcttcc tattggggga agatttacaa
     5521 tgaatgttga agaagctgtt gaagcagcta aacttataaa accttcaatt gcaatcccaa
     5581 tgcattttgg ttctatagta ggagttaatg aagatgctaa tgaatttaaa gacctttgtg
     5641 aagaaaataa tataaagtgt gaaatattgg agaaagaatg aatagattca agaaaatatt
     5701 tatttcttag atttaatcta aataaatcat caaatattta aattaatctt tcatagtcat
     5761 ttaatgaaag agagtgtgat taagaaggtt tatattaaaa aattaacaaa aatctctggt
     5821 tttaaagtat ggattgttaa tggaaaatat ataaggggca acattgacag agaatttact
     5881 aattatggtc aatattatag gtttaggttt atacctaagg acgagttttg gatagataag
     5941 gaaaataatc ctggagaggc aaggtatttt attgatacta tgtttattat gaacagactt
     6001 ctttctaaag gtatggatta taaatctgct cgtaaaattg caaatagaat tgaaaaaaga
     6061 gagagaagta aatctaaact aattcaaaaa catattggta taaaaaagaa taaaggaaat
     6121 atttttaaaa gtgtatataa agaattaata aaagaatata gtaaggggaa gattaaagtt
     6181 tggattgtta atggagaatt ggttagagga ttgttttttt tggattttac tgaaggagga
     6241 cacgataaag tttatagttt tataccgagt aatgagatat ggctagatga tgacttagac
     6301 ccttctgaaa taaaatttgt tttattgcat gaggttcatg aaaggaattt gatgagtaaa
     6361 ggaatgggtt atgagaaggc tcataaatct tctagtgaaa tagaatatta ttgtagaaaa
     6421 catccagaaa aacttgataa atacttaatg aaagaattta agaaagctaa gaagattaag
     6481 gaataatatg ccaagagata gatttgttca aagaaaaaat gcagtgcttt caaaaataga
     6541 taggtcttct gcaggagaat gggacgataa gattaaaaag ttatgtgaaa agataaataa
     6601 aacttctgat ttctatacta ctagtagttg ttctggaaga gttattttaa tgatatctaa
     6661 agataaaaaa ggaaaagatt tgtttttgaa aatatggcat gagaaagtgt attttgatga
     6721 ttttaaagaa attttgaatt cattgttaaa taaaaatgat attattaagt ttaagttaga
     6781 gccccctatc ttacatattg cttgtaggga tttaaaaaaa gcaacatata ttttggagaa
     6841 agcaaaattt cttggtttta aaagaagtag tattcttact tttgacagaa atatagttgt
     6901 ggagataaat agcagtgaga gatttgagtt tcctataatt aaaaataata aaatacttgt
     6961 agacgatgaa tttttgaaac ttgttcttag tatgtctaat tataagatgg aaagaggatg
     7021 ggagaaaatt aaaagattag aaaaagcaat ttaatttata ttttatttaa gatttgtttt
     7081 attgaaaata tattgtattt ttctcatttt atagtaatgt tatctatttt ttatttccat
     7141 aattcttata aatagaggtt gctacttttt tatatgaaaa tctgcttagt gggtttatat
     7201 agagttgaaa ggaggataaa aaagagatgg ttttagattt tgcaaaagaa gcaatagcaa
     7261 atactgctac acacagcatt aactttgatg aattaaaagc tggaaaagct aacataaaag
     7321 ttattggttg cggaggtatg ggtagtaacg ctgtaacttg gctttttaat aaaggtataa
     7381 atggtgctac aatctatgct tgtaatacag atgctcttca tctaagtgta actaaggctg
     7441 acgagaaaat ccttattggt aaggaattga caagaggtct tggtgcaggt ggaaaaccaa
     7501 aaataggtag agaagctgct aaagaagctt tgtctgaact taagagggca gtaggaagtg
     7561 cagatatggt ttttgtattg gctggtatgg gtggtggaac aggaactggt tctgctccag
     7621 ttgtatccca gatagctaag gaatcaggtg cagttgttat cggtgttata acaatgccat
     7681 ttgaatctga aaaagctaga attgataagg cagaatttgg attgcaagaa ttaagagaag
     7741 ttactgatac ttgtattgta ttagataata acagactcgt tgatatcgca ggtcaattac
     7801 caattgagca agcatttgca gttgcaaatg agcttgtatc tactatgatt aagggtattg
     7861 ttgaaacaat tacattgcct tcattaatta atcttgatta tgctgatgtt tcagcaataa
     7921 tgaagaatgg tggagtatca gttattggta ttggtgaaag cgactctcag gataaagtta
     7981 atgaagcagt taggcaagcc cttactcatc ctctacttga tgtggattac agaggtgcta
     8041 gcggagcatt gattcatata acttgtggac ctgatttgaa attagaagag tttgatctta
     8101 ttggtagaac agtttcagaa aacttaggta cagaagctca ggttattatt ggtgcaagaa
     8161 ttagtaaaga tttctctgga agagttagag ttatcacaat aatgaccgga gtcaagtctc
     8221 catatgtcct tggaaaggat attgaagaac actattcttc gtctccttcg agggaaatta
     8281 gtgacttggg tatagaggtt tggagataaa ctttgattaa tttttaattt atttttttat
     8341 tttcttgagg ggattttccc ctcataatgt tttttaatag ttttattgta tttttaagaa
     8401 gaatagttgt tataaactaa tttttctatt aatgtttatg ggaaagaacc ttattgaaaa
     8461 tttgtctaag attcttgaac ttgattcttg taaggcaagc atatgtgcaa taaaaggaat
     8521 aactgttatt aatgataata attttgtttt agaaaaaagg ggtttaatta gagattatcg
     8581 aaagacaact ggagattata gaactggatg gtttaaaagt ttatttacag gaaaattaga
     8641 attaactggg aagcagatag aaaattatca gtatgaatat tcaaaagata taagaaatta
     8701 taatttt
//
LOCUS       DAOPCH010000036         8687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_7589_length_8687_cov_4.734592, whole genome shotgun sequence.
ACCESSION   DAOPCH010000036 DAOPCH010000000
VERSION     DAOPCH010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8687
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7589_length_8687_cov_4.734592"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLU55_03395"
     CDS             <1..525
                     /locus_tag="PLU55_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87132.1"
                     /translation="NRNKTGDLTSVQKVLYEFIQKQVENGKSIKEAEKMLEEGLPENL
                     YKESDIFLATNYFLERAITKPDYLSSIREDKEQLNKKFQIPPTNNSNIKQELSGVSLN
                     GKIDSYDEYLFLKFLEKNPNHNLSIVELYKTINLSTRKGNIIKNKLLSKGMIKVYEER
                     NDKGWRKIIRLNTN"
     gene            898..1071
                     /locus_tag="PLU55_03400"
     CDS             898..1071
                     /locus_tag="PLU55_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87133.1"
                     /translation="MKTKDFIIGHLVIPTTNLVSSQSLERRLKISFRDALTIFGKNDF
                     NHITYNFQLIAIP"
     gene            1131..1376
                     /locus_tag="PLU55_03405"
     CDS             1131..1376
                     /locus_tag="PLU55_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87134.1"
                     /translation="MAFVLSPFLEIGGPENPYYSPESPIKFWETLANLVNYLLDSLNI
                     DYNVDQVEVFIMLFFLSLLLGFICKFILKKLEYSNYQ"
     gene            1409..1879
                     /locus_tag="PLU55_03410"
     CDS             1409..1879
                     /locus_tag="PLU55_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87135.1"
                     /translation="MKTIVHEYKVLRIIILLLFSFASIFLISEFYKGEIKDEGRVAIT
                     LATVSAIAGIVLGYILVLGIIRRKIVNCFDNEIKFSTNGTTVVSGKLTVITKGLKIKH
                     ILLEDETFKVEEEREKIIHEELVSEDLNFTLLIGDFLPKRTKAQKRNMLRDNIL"
     gene            2036..2563
                     /locus_tag="PLU55_03415"
     CDS             2036..2563
                     /locus_tag="PLU55_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87136.1"
                     /translation="MKKFVVLFAVFTLAIIAGCKKKVEPVVSPPPPPPIAKVTQQEFM
                     KGLISMVPQLSPGEVKSVTIDKEKVISGESTLNVSLFPLEFIIPAEEKYIVQYMFPSV
                     IWKWDSFQLEVINLEEYFLHREPGERIWILKESPKGTPPFKNIPTLLVENNNGEFWVL
                     NQNGDEMPLRMNWGK"
     gene            2729..2893
                     /locus_tag="PLU55_03420"
     CDS             2729..2893
                     /locus_tag="PLU55_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87137.1"
                     /translation="MKRLHNRFEKLRNDSIFSPLLEKLDRRNNHNVRHKKQDRGKKKR
                     SAFQEKYPLE"
     gene            3035..3274
                     /locus_tag="PLU55_03425"
     CDS             3035..3274
                     /locus_tag="PLU55_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87138.1"
                     /translation="MKIKERLKSLFGVFWNLAGIGTMLLISLSFFFTLILNEGEIYMF
                     PLWIKTVLLIPLLIMGLSSLCLIAILVIQVILGDY"
     gene            complement(3399..4106)
                     /locus_tag="PLU55_03430"
     CDS             complement(3399..4106)
                     /locus_tag="PLU55_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87139.1"
                     /translation="METINKTKDKYIFKTEIEDSLINSIRRYVGQIQLAAIDEVEIIK
                     NDSPLYDETLAHRLGLVPLKQSSKKEGKLKLSSQEKGMVYSGSLKGDFEVVYDKIPLT
                     ILSAGQEIELIANVKIGKGQEHAKFSPGILNYREVSEIHMDKEIAEKLKNSNPNIEIK
                     QKGDKYIAIDDKEEEITDLCEGFAEKYRKKIEVKTNKEMIIALESFGQIDAKSIFTKS
                     IEALKKDLAEVSKKLSK"
     gene            complement(4153..4626)
                     /gene="rpsK"
                     /locus_tag="PLU55_03435"
     CDS             complement(4153..4626)
                     /gene="rpsK"
                     /locus_tag="PLU55_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPJ87140.1"
                     /translation="MSKQEIEEKQITEEESDKKIEESKEKQAVKKKDKDVEAVLNIYT
                     SFNNTIAHVTDASGRTITKVTGGMVTKHSRLKANPTIAMFIAKRISENMKDKGIKSLY
                     VRIRAKTGSTGPGPGANAIIKSLSREGFKIINILDTTRVPHGGPKKKGGRRGRRV"
     gene            complement(4628..5116)
                     /locus_tag="PLU55_03440"
     CDS             complement(4628..5116)
                     /locus_tag="PLU55_03440"
                     /inference="COORDINATES: protein motif:HMM:NF003139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPJ87141.1"
                     /translation="MKRKHKRYSRPKRPFDKARIEEEGEIVNEFGLKNKKEIWKADAK
                     IKTIREKAKKLISAKPEEQQALFDKLNKRGLKVDSISSVLSLDKKDYLKRRLQTIVVE
                     KKLASTFKQARQLIVHKKVLVDGKVVNTPSYIVPVELENKITLKDKKVSKKKQEKEKV
                     EE"
     gene            complement(5118..5636)
                     /gene="rpsM"
                     /locus_tag="PLU55_03445"
     CDS             complement(5118..5636)
                     /gene="rpsM"
                     /locus_tag="PLU55_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPJ87142.1"
                     /translation="MAENKNRHMHQQEKREERLVRILSKDIEGKMKVYPGLTKIKGVS
                     WAFSNAVCKTLNIDKNRTIGSLTEEEIQKISDFIKNPKLPEYILNRRKDIETGDDRHL
                     TGTDLDLRKDFDIKKLRKIKSYRGLRHNTRLPTRGQRTKAHFRYNRKKGSGIKKKGGE
                     SAVKKTYVGVKK"
     gene            complement(5641..6183)
                     /locus_tag="PLU55_03450"
     CDS             complement(5641..6183)
                     /locus_tag="PLU55_03450"
                     /inference="COORDINATES: protein motif:HMM:NF016787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HPJ87143.1"
                     /translation="MAKEEYAIILEYLPHGYPLEKKMMPIAQAIGEKTLVLLELVPRR
                     GVGLKIGEKVYIGEGKRDKVYYILGRLHREKLTESAKHQLGEFIKQVVKEKEKEFVEF
                     FNKSDAINKRIHQIELLPGLGKKHMQEILKQRKEMEFESFQDMKKRIQNLPDPEKAVE
                     KRIFQELTNIERYNLFTNKT"
     gene            complement(6189..6494)
                     /locus_tag="PLU55_03455"
     CDS             complement(6189..6494)
                     /locus_tag="PLU55_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87144.1"
                     /translation="MIKNTTPLSMAESMEYIEKEKKVERPVAEFIKKFVKITPKEAKE
                     LRKKLEALNIIKLNDTNISKIIDILPEKAEEINKIFVDISLDENETKRILDTVKEFK"
     gene            complement(6496..6756)
                     /locus_tag="PLU55_03460"
     CDS             complement(6496..6756)
                     /locus_tag="PLU55_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21e"
                     /protein_id="HPJ87145.1"
                     /translation="MVKRKLVRTRGKLQLSRYFQRFKQGDSVSIVIEKSLNPSIPKRL
                     QGRTGKIDKKIGRSYAVKIKYPTKEKTFIISPIHLKKIKDKE"
     gene            complement(6794..7012)
                     /locus_tag="PLU55_03465"
     CDS             complement(6794..7012)
                     /locus_tag="PLU55_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87146.1"
                     /translation="MAKKCIYCKADIHDDRVLDVCNRCGVGVWGEKMFNAILRNMGEA
                     NDKGDLCNSNISSNEPPAKLGNSRISFM"
     gene            7167..7364
                     /locus_tag="PLU55_03470"
     CDS             7167..7364
                     /locus_tag="PLU55_03470"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HPJ87147.1"
                     /translation="MIIPVRCFSCGKPIGHLWEEYKKRVAQGESSKKVLDELGLERFC
                     CRAIFLGQEDHLELVAKFKKS"
     gene            7398..8405
                     /locus_tag="PLU55_03475"
     CDS             7398..8405
                     /locus_tag="PLU55_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPJ87148.1"
                     /translation="MAIILLIPFVFSSEENMQKTINISELSLDEKIGQLMFVKPSGKN
                     INYLKELHVGGIFLNNLNSKENYIDSIYFYQNNSKIKLFVATDMEGYWNPFNNFYNSS
                     SFGDINSREESYNLGKEHGKILNELGFNLDFSPIVETKNNVWPYRTFTGTPEEIKGKI
                     SGYIEGLHSENILTTAKHYPGGSLIKNPHLIKYKANISLEELSYFDFAISQGIDFIMV
                     GHPIVYGAIDSKGKQSSISPEVIGELKNKFNGVIITDAVTMMGLRISYLFNFKKVYPD
                     LILAGNDIILDTHKNSKYNSLVKRRNELKKAVLEGRVSQDRIDKSVEKILKIKGYQVV
                     Y"
     gene            complement(8420..8534)
                     /gene="rrf"
                     /locus_tag="PLU55_03480"
     rRNA            complement(8420..8534)
                     /gene="rrf"
                     /locus_tag="PLU55_03480"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            8603..8685
                     /locus_tag="PLU55_03485"
     tRNA            8603..8685
                     /locus_tag="PLU55_03485"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8637..8639,aa:Leu,seq:aag)
BASE COUNT         2784 a         1362 c         1150 g         3391 t
ORIGIN      
        1 aatagaaata aaacaggaga tttgactagt gttcaaaaag ttctttacga atttatacaa
       61 aaacaagtag agaatggaaa atccataaaa gaggcagaaa agatgcttga agaagggctt
      121 cctgaaaatc tttacaagga atcagatata ttcttagcaa caaattattt tttagagagg
      181 gccataacaa agccagatta tttgagttca attagagaag ataaagagca attaaataaa
      241 aaatttcaaa tacctccaac caacaattct aatattaaac aggaattaag tggcgtctct
      301 ttaaatggca agatagattc atacgatgaa tatctttttt tgaaattcct agagaaaaat
      361 ccaaaccata atttaagtat agtagaatta tacaaaacta ttaatctttc tacaagaaaa
      421 ggaaatatta ttaaaaataa gctattgtct aaaggaatga ttaaagtata tgaagaaaga
      481 aatgataaag gatggagaaa gataattagg ttaaacacaa attaaattaa gttaaaatta
      541 aaaacaaata atattcacca attaaatcaa ccaataacaa gacaaacaac atttcaaata
      601 aactcaaaat cgaacataca aacagcataa aagatttcaa taaaatgaaa ttaatgctga
      661 ttaaattaaa agattttgta tatatttgat ttgaacttca ccttgttgat tcaaacaatg
      721 tgaaatatct acaatgtcga actagacact gtataaaacc aaacagaaga cataagaaga
      781 acacaattct taaaactaaa tgtaatctta accatatttc ttacctgaaa taccctgaaa
      841 taccacaaaa taacgtcgat atagcttaaa taatagtatt aactaaaata tcaaaaaatg
      901 aaaacaaaag attttattat tggacattta gtaatcccca caactaattt ggtttcttcc
      961 caatctcttg aaagaagatt aaaaatatct ttcagagatg ctcttaccat ttttggtaaa
     1021 aatgatttca atcatattac atataacttt caattaatcg ccataccatg aaaactttcc
     1081 taattaaatt tttaaatgtg gtcatgtatt gctcttttgg agcaagtttt atggcctttg
     1141 ttctttctcc ttttttggaa ataggaggcc cagaaaatcc atattattct ccagaatctc
     1201 caattaaatt ctgggaaact ttagctaatc ttgtgaatta tcttttagat agtttgaata
     1261 tagattacaa tgtggatcaa gtagaggttt ttattatgtt atttttccta tctttacttc
     1321 ttggatttat ttgtaagttt atactaaaaa aacttgaata ctcaaattat cagtaataat
     1381 ttaataatta aatattggag gacaaaaaat gaaaacaatt gttcacgaat acaaagtatt
     1441 gaggataatt atattgttat tattctcttt tgcttcaatc tttttaatct cagaattcta
     1501 taagggagaa attaaagacg aaggaagagt cgcaatcact ttagccactg tttctgcaat
     1561 tgcaggtata gtgttaggat atattcttgt tcttggaatt attcgaagga agattgttaa
     1621 ttgttttgac aatgaaatta aattctcaac caatggaact accgttgtct caggaaaatt
     1681 gactgttatt acaaaaggat tgaaaataaa acatattctc ttagaagatg aaacatttaa
     1741 agtcgaagaa gaaagagaaa aaatcattca tgaagaacta gtaagtgaag atttaaattt
     1801 cactcttcta ataggagatt ttttgccaaa aagaacaaaa gcacaaaaac gtaacatgtt
     1861 aagagataat atcctttaaa tttctaactt gttttgaaca aaacaatata tcctattttc
     1921 tgactaattt tttaaaacca aaaaataact catatttgtt tagaaaaggg ctgaactccc
     1981 atctattgaa atgaatcaaa ataattttat tgtttaacct ttaattttta caatcatgaa
     2041 aaagtttgta gttttatttg cggtttttac acttgctatt atagcaggat gtaagaaaaa
     2101 agttgaacct gttgtatcac cccctccgcc tccaccaatt gcgaaggtta ctcagcagga
     2161 gtttatgaag ggccttattt caatggttcc tcaacttagt cctggagaag tgaaatcagt
     2221 cacaatcgac aaagaaaaag ttatctctgg ggaatctact ctcaacgttt ctctatttcc
     2281 attggagttt ataattcctg cagaagagaa gtacattgta caatacatgt ttccttctgt
     2341 tatttggaaa tgggatagtt ttcagcttga ggttataaat cttgaagagt attttcttca
     2401 cagagaaccg ggagagagaa tttggatttt aaaagaatct cccaaaggaa cccctccttt
     2461 caagaatatc ccaacccttc ttgttgaaaa taacaatgga gaattttggg tgctcaatca
     2521 gaatggagat gaaatgcctt tacggatgaa ctggggtaaa taattttctt ttacctttac
     2581 ctgaaacccc tgattgaatt aattcccttc aggggttttt cttttttaaa ctattttgtg
     2641 aagttgacaa gaacaaggca gataagagag gcgcttgtta attttattat attttttgtt
     2701 taatttttta attttaggag gaatcacaat gaaaagactc cataacagat ttgaaaaatt
     2761 gagaaacgac agcatttttt cacccctctt agagaagttg gatagaagga ataatcacaa
     2821 tgtccgccac aaaaaacaag ataggggaaa aaagaagagg tctgctttcc aggaaaaata
     2881 ccctttggaa taatttctct tatgttttta aaacccctga ttgaatattt ttcccttcag
     2941 gggcttttct tttgaaaata ataattcaca ttcagagtgg aacaagatat aatcaattaa
     3001 tattaataat taaattttat aggagggcaa tattatgaaa ataaaagaaa gattaaagag
     3061 tttatttggt gttttttgga atttagcagg tattggaaca atgcttctaa tatctctttc
     3121 attctttttt acattaattt taaatgaagg ggagatttac atgtttcctc tttggattaa
     3181 aacagttctt ttaatacctt tattaataat gggattatct tctctatgct taatagccat
     3241 attggttata caagtgatac taggagatta ttaataatta ttctacaaag acatttctta
     3301 caagttaaga tttgtctttt ttttgttaaa acatatgaaa ataatttcat atataacaca
     3361 ccaaaaaata tctctctaac ttccaattat ctttttatct acttgcttag tttctttgaa
     3421 acttcagcta agtccttctt caacgcttct atagatttag tgaatatact cttagcatct
     3481 atttgcccaa aggattctaa tgcgattatc atctctttgt ttgtttttac ttctattttt
     3541 tttctgtatt tttctgcaaa gccctcgcat aaatctgtta tctcttcttc tttatcatct
     3601 attgcaatat atttatctcc tttttgtttt atttctatat ttggattaga attttttagt
     3661 ttctctgcaa tttctttatc catgtgtatt tcagaaactt ctctataatt taatatacct
     3721 ggagaaaatt tagcatgttc ttgacctttt cctattttta cgtttgcaat aagttctatt
     3781 tcttgacctg cactaagtat tgtaagaggt attttgtcat atactacttc aaaatctcct
     3841 tttaatgatc cagaataaac cattcccttt tcctgagaac ttaacttcaa ctttccttct
     3901 ttctttgaac tttgttttag aggcaccagc cctaatctgt gtgcaagtgt ttcatcatat
     3961 aatggagaat catttttgat tatctcaact tcatcgattg cagccaattg tatttgtcca
     4021 acataccttc ttatagaatt aatcaaacta tcttctattt ctgttttgaa aatgtatttg
     4081 tcttttgtct tgtttattgt ttccattttt aaactctacg gcctctaaac aaatttattt
     4141 gttttgccgt ttttaaactc tacggcctct tcttcctccc tttttctttg gaccaccatg
     4201 tggaactctt gttgtatcta agatatttat tattttaaat ccttctcttg ataaactttt
     4261 tattattgca tttgcaccag gtccaggtcc tgtgcttcct gttttagctc ttattctaac
     4321 atataatgat tttattcctt tgtctttcat attttcagat attctttttg ctataaacat
     4381 agcaatagtt ggatttgctt taagtctact atgtttagtc accattcccc cagttacttt
     4441 agtaattgtt cttccagaag catctgttac atgtgcaatt gtattattaa aagaagtata
     4501 gatgtttaaa acagcttcta catctttatc ttttttcttt actgcttgtt tttctttact
     4561 ttcttctatt tttttatctg attcttcttc agttatttgc ttttcttcta tttcttgttt
     4621 agacatttta ttcttctacc ttttcttttt cttgtttttt ctttgatact ttcttatctt
     4681 ttaaagttat tttattttct aattcaacag gaacaatgta tgaaggagtg tttacaactt
     4741 ttccatctac tagtactttt ttatgaacta ttaattgtct tgcttgttta aatgtagatg
     4801 caagtttctt ttcaacaaca attgtttgaa gtcttctttt taaataatct tttttatcta
     4861 aagacaaaac agatgaaatt gaatctactt tcaaccctct tttatttagt ttatcaaata
     4921 atgcttgctg ttcttcaggt ttagcagata tcaatttttt tgctttttct cttattgtct
     4981 ttatctttgc atcagctttc catatctctt ttttattttt taatccaaat tcattgacaa
     5041 tttcaccttc ttcttctatc cttgccttat caaatggtct ttttggtctt gaatatcttt
     5101 tatgttttct cttcatttta tttcttcacc cccacataag tttttttaac tgcactctct
     5161 ccacctttct ttttaattcc cgatcctttc ttcctattat atctgaaatg ggcttttgtt
     5221 ctctgacctc tagtaggtaa tctggtatta tgtcttaatc ctctataact ctttatcttt
     5281 cttagtttct tgatatcaaa gtcttttctt aaatccaaat cagttccagt tagatgtcta
     5341 tcatctcctg tttcaatatc ctttcttcta ttcaaaatat attctggtaa ttttggattt
     5401 tttatgaaat cagaaatctt ttgtatttct tcttcagtga gagatccaat tgtcctgttt
     5461 ttatcaatat ttaatgtttt acaaacagca tttgaaaaag cccaagaaac cccctttatt
     5521 ttagttaaac cagggtagac tttcatttta ccttcaatgt cttttgataa aattctaact
     5581 aatctttctt ctcgtttttc ttgttgatgc atgtgacgat ttttattttc tgccatttta
     5641 ttatgttttg tttgtgaaaa gattgtatct ttctatattt gttaattctt ggaatattct
     5701 tttttcaaca gccttctctg ggtctggaag attttgtatt ctttttttca tgtcctgaaa
     5761 tgattcaaac tccatttctt ttctttgttt caatatttcc tgcatatgtt tcttacctaa
     5821 tccagggagt aattctatct gatgtattct tttatttatt gcatcagact tgttgaaaaa
     5881 ttcaacaaat tccttctctt tttctttaac aacctgcttt ataaattctc ctaattggtg
     5941 ctttgcactt tcagtaagtt tttctctatg taatcttcca agaatataat atactttatc
     6001 tcttttaccc tcgccaatat atactttttc ccctattttt aaaccaactc ctcttctagg
     6061 gacaagttca agtaaaacta atgttttctc ccctatagct tgagctatag gcatcatttt
     6121 cttttctaaa ggatatccat gtgggagata ttctaagatt attgcatatt cttcttttgc
     6181 catttttttt atttgaattc tttaactgta tcaagtattc tttttgtttc attctcatct
     6241 aaactgatat caacaaaaat tttatttatt tcttctgcct tttcaggtaa gatatctatg
     6301 attttagaga tgttcgtatc attaagtttt attatattta atgcttctag tttttttcta
     6361 agttctttag cttctttagg agttattttt acaaattttt ttataaattc agcaacaggc
     6421 ctttcaactt tcttttcctt ttcaatatat tccatagatt ctgccatact tagtggagtt
     6481 gtattcttta tcattctatt ctttatcctt tatctttttt aagtgtattg gagagattat
     6541 aaatgtcttc tcttttgtag gatattttat ttttacagca taacttcttc ctattttttt
     6601 gtctatttta cctgttcttc cttgtaatct ttttggaatt gaaggattta atgacttttc
     6661 tataacaata ctaacagaat ctccttgttt gaatctttga aagtatctac taagctgaag
     6721 cttacctcta gttctcacta attttctctt taccatatct tacccagaaa aattctattt
     6781 aaaaaactat ctcttacatg aaacttatcc gagagttgcc caatttagca ggtggttcat
     6841 ttgaagaaat atttgaatta cacaaatcac ctttatcgtt tgcttctccc atgtttctta
     6901 gaatagcatt gaacatcttt tcaccccaaa ccccaactcc acatctatta catacatcta
     6961 aaaccctatc gtcatgaatg tctgctttac aataaataca ctttttcgcc atttcttcac
     7021 ctttttttta agttaatcat cttcttaata aaaattagtt tttaaatttt tgtgtaattc
     7081 catataccct ttttttgatt tttacaatat tttctttgaa aaatcaagat agtaagtttt
     7141 aaaaatatcc tttcagcatt aattatatga taatcccagt tagatgtttt tcctgtggta
     7201 aaccaattgg tcacctctgg gaagaatata aaaaacgagt agctcaagga gaaagttcta
     7261 aaaaagtact tgacgagcta ggacttgaaa gattttgttg tagagcaata tttcttggac
     7321 aagaagatca tttagaattg gttgctaaat tcaagaaatc ttaaatgaat aaaaaactat
     7381 tatatattat tttgtccttg gctataattt tattaattcc ttttgttttt tcttctgaag
     7441 agaatatgca aaaaacaata aatatttcag aattaagttt agatgaaaaa atagggcaat
     7501 tgatgtttgt taaaccttct ggaaaaaata ttaattattt aaaagaactt catgtgggag
     7561 gtatttttct caataatctc aattccaaag aaaattatat tgattctata tatttttatc
     7621 aaaataattc aaaaataaaa ctttttgttg caacagatat ggaaggatat tggaatcctt
     7681 ttaataattt ttataattct tcttctttcg gagatataaa ctccagagaa gaatcttata
     7741 atctgggaaa agaacatggg aagattttaa atgagttagg atttaattta gatttttccc
     7801 caatagttga aacaaaaaat aatgtatggc cttatagaac atttactgga acaccagaag
     7861 aaattaaagg aaaaatttca gggtatatag aaggattaca ttcagaaaac attcttacaa
     7921 cagcaaaaca ttatccagga ggaagtttaa tcaaaaatcc acatttaatc aaatataaag
     7981 cgaatatctc tctagaggaa ttatcttatt ttgattttgc aatttctcaa ggtattgatt
     8041 tcatcatggt gggacatcct attgtgtatg gagcaataga ttctaaagga aagcaatctt
     8101 ctatttctcc agaagttata ggagaactaa agaataaatt taatggtgta ataataactg
     8161 atgctgttac aatgatgggc cttagaattt cttacttgtt taattttaaa aaagtttatc
     8221 ctgatttgat tttagcagga aatgatatta ttttagatac tcataaaaac tctaaataca
     8281 actccttagt aaaacgaaga aatgaattaa aaaaagcagt attagaagga agagtttctc
     8341 aagatagaat agacaaatct gttgaaaaaa tattgaagat taaaggatat caagttgttt
     8401 attgattata ataaaaaagt taagacagct tgttattttc ccatctttcc ggacagtacc
     8461 gataacaaca taagttgctt aacttctgtg ttcggaaagg gaacaggtgt taccaactta
     8521 ctatggccgt ctcaaaattt caattctttt ttggtttata aaccttttta tatccataat
     8581 cttttgattt tttgtcttat ttgcagggat gccggagcgg tcaaacgggc gagattaagg
     8641 ctctcgtgac ttatgtctac gcaggttcga atcctgttcc ttgcatt
//
LOCUS       DAOPCH010000037         8629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_7642_length_8629_cov_6.977490, whole genome shotgun sequence.
ACCESSION   DAOPCH010000037 DAOPCH010000000
VERSION     DAOPCH010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8629
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7642_length_8629_cov_6.977490"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1034)
                     /locus_tag="PLU55_03490"
     CDS             complement(<1..>1034)
                     /locus_tag="PLU55_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87149.1"
                     /translation="AGYYNFTSERQENENYTYIFHQREFIVLKNPEICEIFFNETSPI
                     NYPATFQVWTNCTSPFTLYRNGTEILNNSEQKLVAGTYNFSMFRLDSQNFSYTYDYSY
                     FTIDELLDVTPPYFTQIPNNASLFYKNQSLGVDFDATDNINFGFYKINDTRFSINQTG
                     YLSNSTSLAAGNYAINVSINDSSNNINWTIYTVQVNKSTFYDCGVYFNTLSPITYPET
                     FIAYTNCSSAYTIYRNGTSIPNNSIINSGAGYYNITVQRTDTQNYTNFFSSAFFIVNK
                     GTPTATLSSSAGWELTYPTSTTISSINSLQGDSDLIYRIYRNSTNINTGETTILGVGF
                     YHYILNTTGG"
     assembly_gap    1035..1134
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1135..6017)
                     /locus_tag="PLU55_03495"
     CDS             complement(<1135..6017)
                     /locus_tag="PLU55_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87150.1"
                     /translation="MKKGLIKIGVIGFLSLFLFGIIAFSLFSSAYSNIPEERGNSVIF
                     DFKSNNTYPLDTVLRLDFTKLGNYKLIINSPSKEVISRVSSNDNFLLKLEEEGEYSVV
                     VQRGNGKKVYYFFVISSEQESPEEQKPNPEDPVQEKAEINKPVEWTQTISEENLTNLI
                     EIPPVINLTLTDKTGEEISYTLTNIDGKNYINLTESLSENISGELKIEYTTEPPKIEE
                     TIISNTTKEVVVSSLEGLNYKNVLSYTYLPREIYSKSSIKIYWKENNTYLDFNASDTN
                     GNGLFDYVEWITPHLSSQTFEIIVIIKAEHLDSNKNFISDIYEQVKEKDNLWSEEIRE
                     GEYVRVTFEVPLDNTRDITLFPRIISGNPIVEVYEKDKNELIAQFDNLISNEYNKVYL
                     TNLQSQQDIFDLKVVGGILEFDHIIDPNFYARTGVTYYYAGTPTQITFSTAMPDTNYS
                     ILFSGQQDTDAVFSIQYDNKATTGFRINRVHDDGGANESAGIEWAALSFGEFDFQNIS
                     VKCNSGSGSSTSVINFESSFPDTNYAVVAYTPWDSDSPRVSYSSKSTGSWNSVILDDG
                     GALEDVSSIDWCAVSYGTETVGGITIKAGSNTASGATSKVIPLGSSFPDTNYVVVTTA
                     SRTGTADPCEIEVSGKTTTSFTVTAEDDDTTNNCNGREFNWIAISAGEFLLDTTPPEF
                     IFIPLNASIFYGNESLGVDFDATDNVNFGYYSVNDTRFSINQSGFLTNSTSLSGGNYL
                     LNISINDTSNNINWIFYNVNVLKSNEDCFVYFNTSSPVTYPETFRVYTNCSSNHNLFM
                     NGTQILNNSIIDSGAGYYNFTSERQENENYTYYSDTKFFRIDKNTEVYNVLFNTSSPI
                     YYPETFRVYSNPSSAFTLYRNETIIENNSEQSNGAGYFVYLSQRTDEQNYSLINVQDS
                     FTVLKSQEFCSILFNETFPLSDKNSFLVWTNCSGQVRLYRDSTLIENNSEQFLSGGVY
                     NFSFSRLDTENYSQIYDEKQVIIQDTIIPLINFGTGSTKNNSKFARDWIYVDVEVIEE
                     NEENITFNLYNSNRVLVNSSTYTDLRREINWTNLPEGIYYFNVTVFDKYNHFNSTSTR
                     KTTLNFTLPTINFVPSTEENGASLNRNWIYANVSAANVNEEINVTFGLYNDLGLFNQS
                     IYTDSRREINWTNLPEGMYYYNVTLFDNQDGRVSTLTRSITLDSSPPNIINSLPEDNS
                     IVSHSSQIMIADISDNLGLSNFTFSIYNETHLINQTYQDISGLSALTNIFYDLPHEGV
                     FKWNYNSMDLAGNYFLTPNLSLIYDLTSPLVDYTFPTPDENVTLPLNFLYVNVSVIEG
                     NLANITFNLYDFNQNLLNSSTYTDSRREINWTNLPEGVYYYNVIVFDLANNYNYTSTR
                     QISLDLTAPNATLLFPENNSLLSDSTQNLTVNISDNLGLSNMTLFIYDKTNLINETTQ
                     EISGTEIITGIVYNFVYDGIFKWFYKIFDLAGNSFETEKNTITIDTTAPIMVYGSGTP
                     PDFSRISRNWIYISASINEANFANITFNLYFSNYTLLNSSTYTDSRREINWTNLQEGT
                     YYYNITSFDLLNHSNTLATRRIKIDMSDPNLYTRAGTTTYTAGTPRQITFSTAMPTAD
                     YSILFSGQQDTDAVFSI"
     gene            6159..6317
                     /locus_tag="PLU55_03500"
     CDS             6159..6317
                     /locus_tag="PLU55_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87151.1"
                     /translation="MVEITFKDNKIQKGHRIKIPKAIVDTLNLKEGAKIILKFDPINK
                     KIIVEEEK"
     gene            6314..6634
                     /locus_tag="PLU55_03505"
     CDS             6314..6634
                     /locus_tag="PLU55_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87152.1"
                     /translation="MNKVFLIVIIGAFFFGISGIIFAEEFQEGIKLSEGKNLVYFSEE
                     IKPIYASDLVKIYPEINAITYYEGDEEKGYVNVFGGVGENFVVMFNNTYEITVEKEVT
                     LKLK"
     gene            6631..7824
                     /locus_tag="PLU55_03510"
     CDS             6631..7824
                     /locus_tag="PLU55_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87153.1"
                     /translation="MIKKISLNLAIILFILPLGSAAHYIVGIVENAKDGTSANGQSIV
                     LWNPDIGIQENVSDIIGPAGNSRTNNIYMIDCELLPSGCNVTNTLSLKVINNGDNYIS
                     QEKNVSVGGFGYDIVENITLNSPPNITLVNVDDSITSPQNEIDLIPAETKEVICSAII
                     YEYEGEESLINATGRFFDNVQSNYDSLEDNNEHYRNNSCLIDKNYGGVNEAAVYCSFY
                     IWYYSNSENWNCTIEVTDNLSISSRGSDLSFINPLLALGVESIVTFNLTKSDITDEAI
                     LDVTNYGNVKINLSLSGYAFEQNDGLAMNCSEGEIRNIPVENEKYNLTNSNSGDITFE
                     EFMISYKNLTSYPAVNKFNLEYRKDDLLNEATKSTYWRIHVPDGITGNCQGNIVFGAV
                     RAPGD"
     gene            7825..>8629
                     /locus_tag="PLU55_03515"
     CDS             7825..>8629
                     /locus_tag="PLU55_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87154.1"
                     /translation="MNNKIRIGIKTGIITLIIIVNLLYVSSANIGVSPANVYFKDVLR
                     GGYAERIVTITVDSEEPTKISLEPRGEIVDWISFEEKEFEVSKNKPYYLKIILQPPVD
                     IPNGNYTGFLRVTTSSKNKGVEGQATGVINAALDLYLEVKITDVEFSSCRAWDFQVES
                     VEKGDDILFKVKVLNEGNVRLYPTIKIDMWDQERTELVKQVEFSEDMIIPTTEKELNI
                     KVESSDMNLGQYWAEITSIECYAAQTLTFDILEEGALMAQGNLLRIISPT"
BASE COUNT         2983 a         1135 c         1376 g         3035 t
ORIGIN      
        1 cacctcctgt tgtattaaga atataatgat aaaaccctac tcctaagata gttgtttccc
       61 ctgtattaat atttgtacta tttctatata ttctataaat caaatcagaa tctccttgaa
      121 gagaatttat tgaagaaatt gttgtacttg taggataagt taattcccat cctgcagaac
      181 ttgaaagagt tgctgtagga gttcccttat ttactatgaa aaacgctgag gaaaagaaat
      241 ttgtatagtt ttgtgtatct gttctttgta cagttatgtt gtaatatcca gctccagaat
      301 ttattattga attatttggg attgaggttc catttctata aattgtgtaa gctgaagaac
      361 agtttgtata agcaatgaaa gtttctggat atgttattgg gcttaaagta ttgaaataaa
      421 ctccgcaatc ataaaaagtt gatttgttta cttgaactgt gtaaattgtc caattaatat
      481 tgtttgaaga atcatttatt gaaacattaa tcgcataatt tcctgctgca agcgaggttg
      541 agtttgaaag atatcctgtt tgatttattg agaaccgagt gtcattaatt ttgtaaaatc
      601 caaaatttat attatctgtt gcatcaaagt caactcctaa agattgattt ttataaaaca
      661 aagaggcatt gtttggaatt tgtgtaaaat aaggaggagt tacatctaac aattcatcga
      721 ttgtaaaata gctataatcg taagtatatg aaaaattctg tgaatctaat ctaaacattg
      781 aaaaattgta agttcctgca accaattttt gttcagaatt gtttaaaatc tctgtaccat
      841 tcctatacaa tgtaaatgga gaggtacagt ttgtccaaac ttgaaatgtt gctggataat
      901 ttattggaga agtttcattg aaaaatatct cgcaaatctc tggatttttt aaaacaataa
      961 attctctttg atgaaatata taagtataat tttcattctc ttgtctttca gaagtaaaat
     1021 tataatatcc tgctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgaata
     1141 gaaaaaacag catctgtatc ttgttgtcct gaaaataaaa ttgaataatc tgcagttggc
     1201 atagccgttg agaaagtgat ttgtctaggg gttcctgcag tataagtggt ggtgcctgca
     1261 cgagtgtata aattagggtc tgacatatct atttttattc ttctggttgc aagagtattt
     1321 gaatggttca acaaatcaaa agaagtaata ttataataat aagtaccctc ttggagattt
     1381 gtccagttta tttctcttct tgaatctgtg tatgttgaag aatttaataa agtgtaattt
     1441 gaaaaataca aattaaaggt tatatttgcg aaattagctt catttattga tgcgcttatg
     1501 tatatccaat tacgagaaat tctagaaaaa tctggagggg ttcctgaacc ataaaccata
     1561 attggtgcag tagtgtcgat tgtgattgtg tttttttctg tttcaaaaga atttcctgct
     1621 aagtcaaata ttttataaaa ccatttaaat attccgtcgt atacaaaatt gtaaacaatt
     1681 cctgtgatta tctctgttcc agaaatctct tgagttgtct catttatcaa gtttgtttta
     1741 tcgtatataa atagagtcat attggataat cccaaattat cagaaatatt gacagtcaaa
     1801 ttttgagttg aatctgataa aagagaatta ttttcgggaa acaatagagt tgcattagga
     1861 gcagttaaat ccaaactaat ttgccttgtg gaggtgtaat tataattatt tgccaaatca
     1921 aatacaatta cattataata ataaacaccc tctggtaaat ttgtccaatt tatttctctt
     1981 cttgaatctg tgtatgttga agaattcaat aaattttgat taaaatcata caaattaaaa
     2041 gtaatatttg ctaaatttcc ttcaataact gaaacattca cgtataaaaa atttaacgga
     2101 agagttacat tttcatctgg agttggaaat gtataatcca ccaaaggaga agttaaatca
     2161 tatattaatg ataaattagg tgttaaaaaa taatttcctg ctaggtccat agaattataa
     2221 ttccatttga aaaccccttc atgaggtaaa tcataaaaga tattcgttaa ggcagataat
     2281 ccagaaatat cttgataagt ttgattaatt agatgagttt cattgtaaat agaaaaagta
     2341 aaattagata gtccaaggtt atctgaaata tctgcaatca ttatctgaga tgaatgcgaa
     2401 acaatagaat tatcttcagg aagagaatta attatgtttg gaggggatga atctaaagta
     2461 attgaacgag ttagtgttga aactctaccg tcttgattgt caaacaaagt tacattgtaa
     2521 taatacatcc cttctggaag atttgtccaa tttatttctc ttcttgaatc tgtataaata
     2581 gattgattaa ataatcctaa atcattatac aatccaaatg tgacatttat ctcctcgttt
     2641 acattagcgg cacttacatt tgcataaatc caatttctat ttagagatgc tccattttcc
     2701 tctgttgaag gaacaaaatt aatagttgga agtgtaaaat ttaaagttgt ttttctggtt
     2761 gaagtggagt tgaagtggtt gtatttatca aatactgtta cattaaaata atatattcct
     2821 tctggaagat ttgtccaatt tatttctctt cttaaatctg tgtatgttga agaattaacc
     2881 aaaactctgt ttgaattata aagattgaat gttatgtttt cttcattttc ttctattact
     2941 tctacatcaa catatatcca atctcttgca aactttgaat tgttttttgt ggagcctgtt
     3001 ccaaaattaa ttaaaggaat tattgtatct tgaattatta cttgtttttc gtcatatatt
     3061 tgagaatagt tttctgtatc taatcttgaa aatgaaaaat tatatactcc tccagataaa
     3121 aactgttcag aattattttc aattaaagta ctatctctat ataacctaac ttgtcctgaa
     3181 cagttagtcc aaactaagaa ggaattttta tcgctaagtg gaaatgtttc attaaaaagg
     3241 attgaacaaa attcttgact ctttaataca gtaaaagaat cctgaacatt tatcaaagaa
     3301 taattttgtt catctgttct ttgtgaaaga tatacaaaat aacctgctcc attggattgc
     3361 tctgaattat tttctataat agtctcgttc ctatataatg taaatgcaga tgaaggatta
     3421 gaataaaccc taaatgtttc tggataataa atcggggaag aggtattaaa taaaacatta
     3481 tacacttctg tatttttatc tattctgaaa aattttgtat ctgaataata agtataattt
     3541 tcattctctt gtctttcaga agtaaaatta taatatcctg ctcctgaatc tattattgaa
     3601 ttatttagta tttgtgttcc attcataaat aagttatgat ttgaactaca atttgtatat
     3661 actctaaatg tttccggata agttactgga gaagatgtat taaaataaac aaaacaatcc
     3721 tcatttgatt taagaacatt aacattgtaa aaaatccaat taatgttgtt tgatgtgtcg
     3781 ttaatagaaa tattcaataa ataatttcct cctgataaac ttgtggaatt ggttaaaaat
     3841 cctgattgat ttattgaaaa tctagtgtca ttaacagaat aatatccaaa atttacatta
     3901 tctgttgcat caaagtcaac tcctaaagat tcatttccgt agaagattga agcatttaat
     3961 ggtataaata taaactcggg aggagttgtg tccaataaaa actctcctgc tgaaattgct
     4021 atccaattga attctcttcc attacaatta tttgtggtgt catcatcttc tgctgttaca
     4081 gtgaaagagg ttgttgtttt tccagagacc tctatttcac aagggtctgc tgtacctgtt
     4141 cttgatgctg ttgttacaac cacgtaattt gtatctggaa aagaacttcc caacggaata
     4201 acttttgaag ttgctccaga agcagtattg ctacctgctt taattgttat tccacctact
     4261 gtttctgtac cataagaaac agcacaccaa tctatactgc taacatcttc taatgctcca
     4321 ccatcatcta aaataactga attccagctt cctgttgatt ttgaagaata acttactctt
     4381 ggagaatcag aatcccaagg tgtataagct acaacagcat aatttgtatc tgggaaactt
     4441 gattcaaaat ttattacaga tgtgcttgaa cctgaacctg aattacattt aactgaaata
     4501 ttctgaaaat caaattcgcc aaaagataat gctgcccact caatccctgc agattcgttt
     4561 gctccgccat catcatgaac cctgtttatt ctaaagccag ttgttgcttt gttatcatat
     4621 tgaatagaaa aaacagcatc tgtatcttgt tgtcctgaaa ataaaattga ataatttgta
     4681 tctggcatag cagttgagaa agtgatttga gtaggagttc ctgcataata ataagtcact
     4741 ccagtacgag catagaaatt cgggtctata atatgatcaa attccaaaat acccccaact
     4801 acctttaaat caaatatgtc ttgttgagat tgaagatttg ttaaataaac tttattatat
     4861 tcgtttgaaa taagattatc aaattgtgcg attaactcat ttttatcttt ttcataaact
     4921 tcaactattg gatttcctga gataattctt ggaaataatg ttatatctcg tgtgttgtct
     4981 aaaggtactt caaaagtaac tctaacatat tctccttctc ttatttcttc tgaccagaga
     5041 ttatcttttt cttttacttg ctcataaata tctgaaataa aatttttatt tgaatctaag
     5101 tgttctgctt tgattattac aattatttca aatgtttgag atgacaaatg gggagttatc
     5161 cattctacat aatcaaataa gccattacca tttgtatctg aagcattgaa atctaagtag
     5221 gtattgtttt ctttccaata tattttgatt gaagattttg agtatatttc tctaggtaag
     5281 taagtataag agagaacatt cttgtaattg agtccttcta atgaggaaac tacaacttct
     5341 ttggttgtgt tagaaattat tgtttcttct atttttggag gttctgtagt atattctatt
     5401 ttaagttccc cagaaatgtt ttcagataga gattctgtta aattaatgta atttttgcca
     5461 tcaatgtttg tcaaagtata tgaaatttct tcacctgttt tatctgttaa agttaaatta
     5521 attactggcg gtatctctat taaatttgtt aaattctctt ctgaaattgt ttgtgtccat
     5581 tctactggct tatttatttc tgctttttct tgaacagggt cttctggatt aggtttttgt
     5641 tcttcaggag attcttgttc tgatgaaata acaaaaaaat aataaacctt tttcccgttt
     5701 cctctctgaa caactacaga gtattctcct tcctcttcta gtttaagtaa aaaattatcg
     5761 tttgatgaaa ctctagagat aacttcttta gatggagaat ttataattaa tttgtaattg
     5821 cctaattttg tgaaatctaa cctcaaaaca gtatctaagg gataagtatt atttgattta
     5881 aaatcaaaaa ttacagaatt tcctctttct tctggaatat ttgaatatgc tgaagaaaaa
     5941 agagagaatg ctattattcc aaaaaggaac aaagacagaa agccaattac acctatttta
     6001 attaaccctt ttttcatttg attacgcaaa tttagatatt tttatagatt attgttatac
     6061 aaaatctctt ataatggtaa gatttaaata cttttaaaag tatctgttta gtatcaaaag
     6121 agggtaatta ggcactatta aaatcctttt aaaagtacat ggttgaaata acatttaaag
     6181 ataataagat acaaaaaggc cacagaatca aaattccaaa ggcaatagta gatacactta
     6241 atctaaaaga gggtgcaaaa ataattttga aatttgaccc aataaataaa aaaataattg
     6301 tggaggaaga aaaatgaata aagtattctt gatagtaatt ataggggcat tcttttttgg
     6361 aatcagtggg attatttttg cagaagaatt tcaagaagga attaaacttt cagagggaaa
     6421 aaatttagtt tattttagtg aagagattaa accaatatat gcaagtgatt tggttaaaat
     6481 ttatcctgaa ataaatgcta ttacttatta tgagggagat gaagaaaaag gatatgtaaa
     6541 tgtgtttgga ggggttggag aaaattttgt tgtaatgttc aataacactt acgaaataac
     6601 tgttgaaaag gaggtaacat taaaattaaa atgataaaaa agatttcatt aaatctggca
     6661 attatcttgt ttattttgcc attgggctct gcagcgcatt atattgttgg aattgttgaa
     6721 aatgcaaaag atggaactag tgctaatggg cagagtatag ttctttggaa tcctgatata
     6781 ggaattcaag aaaatgtaag cgatataatt ggtcctgctg gaaactccag aacaaataat
     6841 atctatatga ttgattgtga acttctacca agtggatgta atgttacaaa taccttaagt
     6901 ttaaaggtga taaataatgg ggacaattat atttcccaag aaaaaaatgt tagtgttggg
     6961 ggatttggat atgatattgt agaaaatata accttaaatt ctcctccaaa tatcactctt
     7021 gtaaatgtgg atgattcaat tacttctccg caaaatgaaa ttgatttaat acctgctgaa
     7081 actaaagaag taatctgttc tgctattatt tatgaatatg agggagaaga gtcattgata
     7141 aatgcaactg gaagattttt tgacaatgtt caatcgaatt acgattcctt agaagataat
     7201 aatgagcatt atagaaacaa tagttgttta atagataaaa attatggagg agttaatgaa
     7261 gctgctgttt attgtagttt ttatatttgg tattattcaa attctgaaaa ctggaattgt
     7321 actatagaag taacagataa tttatctata tcttctagag gtagtgattt atctttcata
     7381 aatcctcttc tagctttagg agtggaatca attgtgacat ttaatctaac aaaatctgac
     7441 ataactgatg aagcaatatt ggatgttaca aactatggga atgttaaaat aaatctatct
     7501 ttaagcggat atgcttttga acagaatgat ggtttagcta tgaattgtag tgaaggtgaa
     7561 atcagaaata tacctgtaga aaatgaaaaa tacaatttaa ccaatagcaa ttcaggagat
     7621 ataacatttg aagagtttat gatatcttat aaaaatttga caagttaccc tgctgtaaat
     7681 aaattcaatc tagaatatag aaaagatgat ttactaaatg aagccaccaa atcaacatac
     7741 tggaggattc atgttcctga cggtataact ggaaattgtc agggaaatat tgtttttgga
     7801 gctgtaagag caccaggaga ttaaatgaac aacaaaataa gaataggtat caaaacagga
     7861 atcattacgt taattataat tgtaaattta ttatatgtct cttctgcaaa cataggcgtt
     7921 agtccagcaa atgtttattt caaggatgtt ttaagaggag gttatgctga aagaatagtt
     7981 acaataactg ttgattctga agaacctaca aaaatatctc ttgaaccaag aggggagatt
     8041 gttgattgga tttcatttga agagaaagaa tttgaagttt caaaaaataa accatattat
     8101 ttaaaaataa ttttacagcc tcctgttgat atacctaatg gaaattacac tggtttctta
     8161 agagtaacta cctctagcaa aaataaagga gttgagggac aggccactgg agtaattaat
     8221 gctgcattag atttatactt agaagtaaaa ataacagatg tggaattctc ttcttgtaga
     8281 gcgtgggact ttcaagtaga atctgtagaa aaaggagatg atattttatt taaagttaaa
     8341 gtgttaaatg aaggaaatgt taggctttat cctacgataa aaatagacat gtgggatcaa
     8401 gaaagaacag aactagtcaa acaagtagaa ttttctgaag atatgataat tccaacaact
     8461 gaaaaggaat taaatataaa ggtggaatct tctgacatga acttggggca atattgggca
     8521 gaaataacct ctattgaatg ttatgctgct caaacattaa cttttgatat tttagaagaa
     8581 ggtgctttga tggcacaagg aaatctttta agaatcattt ctcctactt
//
LOCUS       DAOPCH010000038         8199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_8064_length_8199_cov_5.391699, whole genome shotgun sequence.
ACCESSION   DAOPCH010000038 DAOPCH010000000
VERSION     DAOPCH010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 8199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8199
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8064_length_8199_cov_5.391699"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /gene="rplD"
                     /locus_tag="PLU55_03520"
     CDS             <1..720
                     /gene="rplD"
                     /locus_tag="PLU55_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPJ87155.1"
                     /translation="DIVAKVIETKKKQQPYSNSPVAGKQHSAKGLIVHARHVWRSGYG
                     RGQSRVPRKIFSRRGSQFNWQGAEVPHAKGGMRAHPPKITQFVRDLKINKKELKIALF
                     SSISATASEKYILKKYSTIKKLDKKAPFIVSSDLTKKKTKDILSELKTILGNLYPVSI
                     KEKSIRAGKGKLRGRKYKSNAGMILIIGKNENVKTGLFDVRKANELSVSDLAKGGLGR
                     VAVYTEEAVKELENKMEGKKK"
     gene            717..959
                     /gene="rplW"
                     /locus_tag="PLU55_03525"
     CDS             717..959
                     /gene="rplW"
                     /locus_tag="PLU55_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPJ87156.1"
                     /translation="MKLKPIATEKAIMKVERDNIISFLVDENSTKAEIEKEIENLFKV
                     KVEKIRTSVRNNQKIAYVKLNKKYLAIDLATKLGLM"
     gene            960..1655
                     /gene="rpl2p"
                     /locus_tag="PLU55_03530"
     CDS             960..1655
                     /gene="rpl2p"
                     /locus_tag="PLU55_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011250490.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPJ87157.1"
                     /translation="MGKRIIQQARGHGSSTYRAKPTAFVYKIRYPKKLEGEGTVVKIL
                     HSPAHSAPIAKVSYSGGSFYIPAFYGMIEGQKISFNEGIKPGNILPLEKIPIKTEIYC
                     IESRPGDGGKFIKAGGNVAVVTRIIGDKIMVMLPSKREKSFNGRCRAVIGKIAGHGRL
                     EKPIVKAGKNYYIKKARNKLWPRTSAVKMNAIDHPLGSGRGKNIKSKIAKRNAPAGRK
                     VGLIRPRRTGKKK"
     gene            1657..2052
                     /locus_tag="PLU55_03535"
     CDS             1657..2052
                     /locus_tag="PLU55_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPJ87158.1"
                     /translation="MEIQKKQFTYRGKTVDELKELEVREFAKYLPSREKRTVLRNFQM
                     IEDFVKRAKTKIEKGKKIRTHSRNIIIVPKMIGMTIHVHNGKNFNQVHIEPEMLGHRL
                     GEMSGTRSKVAHSKAGVGATKGSKHKAKK"
     gene            2058..2693
                     /locus_tag="PLU55_03540"
     CDS             2058..2693
                     /locus_tag="PLU55_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL22 family ribosomal protein"
                     /protein_id="HPJ87159.1"
                     /translation="MTEKNYNPNQKEKNSMKKQPKTKIIENPIKTENKAETENKKNED
                     TTKEKTQVVEEKKTKEDKKEIKKQVKKIKKEEVYVNAKSVPISTKYAVNLCKFVKGKR
                     IGDAIRDLEQVVLLKKAVPMKGEYSHKKGKGMSGGAYPQKAAKHFIILLKSLAGNANN
                     HEIIEPIIVEARANWAPAPRGRFGRVKRKRTHITLKAVEMKIKENKKENKK"
     gene            2696..3370
                     /locus_tag="PLU55_03545"
     CDS             2696..3370
                     /locus_tag="PLU55_03545"
                     /inference="COORDINATES: protein motif:HMM:NF003219.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPJ87160.1"
                     /translation="MEEKQTVKFKKEEFAIKESVKKMLGKGKVSKVRIEYTPVGEKII
                     ISTNKPGLIIGKGGEKLNEITEYLRKHFKFENLHLDVEEITEPEFDAQIVADEIALGL
                     ERFGPLKFKILAYRSLEKVMKAGALGCEIRLSGKLPSSRAKTWRFAQGYLKKTGDSSK
                     VVDRAQSRAETKPGTVGIKVSILSPRAVLKDKIFINEELKEKLRMNQQELEDKPVKKS
                     KRSKKD"
     gene            3373..3564
                     /gene="rpmC"
                     /locus_tag="PLU55_03550"
     CDS             3373..3564
                     /gene="rpmC"
                     /locus_tag="PLU55_03550"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPJ87161.1"
                     /translation="MKYKEIKNMSDEERNKKLNDLKIELLKSRTKSSKTGSSNTREIK
                     RTIAKILTFNTSKKGIEKK"
     gene            3568..3867
                     /gene="yciH"
                     /locus_tag="PLU55_03555"
     CDS             3568..3867
                     /gene="yciH"
                     /locus_tag="PLU55_03555"
                     /inference="COORDINATES: protein motif:HMM:NF002096.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HPJ87162.1"
                     /translation="MEIDPKTGLPLQAIEWENLTRSSQRISVTTDKKRYGKIVTLVSG
                     FDKDVDIKKIAKALKNELACGGTFKNNVIELQGDHMKKIKPLLIKMGFENNSIED"
     gene            3889..4254
                     /gene="rpsQ"
                     /locus_tag="PLU55_03560"
     CDS             3889..4254
                     /gene="rpsQ"
                     /locus_tag="PLU55_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPJ87163.1"
                     /translation="MERKQKTKARSKDTSKKNQEEKKIASAENDKKITTRGRIFEGIV
                     TKKFPKRIVLEFERMVYIRKYERYAKMKIKMHARLPDYIEKEINIGDLVRIRECRPLS
                     KIIHFVVIEKIKDASEANK"
     gene            4251..4652
                     /locus_tag="PLU55_03565"
     CDS             4251..4652
                     /locus_tag="PLU55_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011153090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HPJ87164.1"
                     /translation="MKAISARGTSSLNLGAEVIAADNSGAKIVKIVSVKKSKAKKGKQ
                     VSAKIADWVKVSVRKGVPDMKGKVFDAVVIRQKKPFRRLSGERIAFEDNAIAILKDEK
                     GNPKGTQIKGPIAREVQERWAQVAKIASVVY"
     gene            4654..5088
                     /gene="rplX"
                     /locus_tag="PLU55_03570"
     CDS             4654..5088
                     /gene="rplX"
                     /locus_tag="PLU55_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012571035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPJ87165.1"
                     /translation="MKKKFSTQWKSSKQPRKKRKYVANAPLHLRKKFVSINLSKELRK
                     KTNKRNIPAKKGDKVKICVGKFKGKTGKILTVNLKTSKIIVEEIQVKKQDGSKVNFKL
                     QPSNLQIIELAERKSNKKNKQKEEKKESSGNKNKEKGKENKK"
     gene            5085..5735
                     /locus_tag="PLU55_03575"
     CDS             5085..5735
                     /locus_tag="PLU55_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW motif-containing protein"
                     /protein_id="HPJ87166.1"
                     /translation="MSHIKRQIIPKSWPIKRKGTAFVVKPTLGFSNSLPILIILRDLI
                     NVAQNRKEVKKMINMKSILINGREVRDEKEGVLLFDVITLVPSKKHYRLSIFKNGKLN
                     VEEIKESEANTKISKIVNKKILKGKKTQLNLSDGRNLLSEIKCNVNDSVLINLKTKKI
                     EKCLPFTENSEVIVFSGKHAGEKGKIEKINPERKNAEIKLEKGNINVLIKQLMVIN"
     gene            5755..6240
                     /gene="rpl5p"
                     /locus_tag="PLU55_03580"
     CDS             5755..6240
                     /gene="rpl5p"
                     /locus_tag="PLU55_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012877.2"
                     /note="part of 50S and 5S/L5/L18/L25 subcomplex; contacts
                     5S rRNA and P site tRNA; forms a bridge to the 30S subunit
                     in the ribosome by binding to S13; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPJ87167.1"
                     /translation="MRKIKIEKVVLSVSGTADGLDKGFRLLKFLTDKKPAKIKSKRRI
                     PTWGVRPGLEVGAVVTLRKDFEQFLKRLLVSVDNTLKRKQISENNFSFGIKEYIEIPG
                     AEYQRDIGIIGLDVTVVFKRSGRRVGLRKIKRGKVSKKQTITREEIIKFMEDNFQTKF
                     I"
     gene            6311..6472
                     /locus_tag="PLU55_03585"
     CDS             6311..6472
                     /locus_tag="PLU55_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HPJ87168.1"
                     /translation="MKHNVPKKRKIGIAAQKCERCGRFGAHIKSYGLNLCRHCFREIA
                     SEIGFKKYS"
     gene            6474..6854
                     /locus_tag="PLU55_03590"
     CDS             6474..6854
                     /locus_tag="PLU55_03590"
                     /inference="COORDINATES: protein motif:HMM:NF003115.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPJ87169.1"
                     /translation="MSHDVVADALNMIRNAKKAGKENVEIKIISNLLIEILKIMKQEK
                     AIKKYKIDAKEKSITLTIGELSECKAVKPRFSCDKDQVEKYRRRYLPARNLGTLIIST
                     NKGLMTHKEAQEEGTGGCVIAYFY"
     gene            6856..7398
                     /gene="rpl6p"
                     /locus_tag="PLU55_03595"
     CDS             6856..7398
                     /gene="rpl6p"
                     /locus_tag="PLU55_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPJ87170.1"
                     /translation="MKKEFSQEIEIPEGIEVKKEDKKIIVKGPEGEVSKEFNFGRIKF
                     ENKDGKILISYPMATRNDKRMINTITAHLKNMVKGVKEKYEYKLKICSSHFPINVEIK
                     GNEALIKNFLGEKTPRKCKIPQDVEVKINKEIITIKSANKELAGQSAANFEMATAVRN
                     RDRRVFQDGVYIINKDGEDI"
     gene            7400..7807
                     /locus_tag="PLU55_03600"
     CDS             7400..7807
                     /locus_tag="PLU55_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="eL32 family ribosomal protein"
                     /protein_id="HPJ87171.1"
                     /translation="MPKFLRHTSHKYSKLGLRRKKKQVWRRPTGRDNKMREQRKGKPA
                     IVAIGYSTNKKEKGKINNKTPVVVNNVRQLENIQKNQIVVLGRIGKKNKIEIAEKANE
                     LKIEIYKFNPQKYLKMNKKKDKKIEKKEKENKK"
     gene            7804..>8199
                     /locus_tag="PLU55_03605"
     CDS             7804..>8199
                     /locus_tag="PLU55_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HPJ87172.1"
                     /translation="MNLRSKKQLAANTFGVGKSRIMFVNERIEEIKEAITKQDMRNLL
                     QDGAIIIKEVKGRSKNVGKKKKRKFSKRRKNVNTRKKDYVILTRKLRKLIADRKQKGE
                     INKEEEKKLRNRIRNKMFKSKAHLKEYMGG"
BASE COUNT         3850 a          877 c         1567 g         1905 t
ORIGIN      
        1 gatattgttg caaaggttat tgaaacaaaa aagaaacaac aaccttactc aaattctcca
       61 gtagcaggaa aacaacattc agcaaaggga ttgattgtgc atgcaagaca tgtatggaga
      121 agtggatatg gaagagggca atcaagagta ccaagaaaaa tattctctag gagaggttct
      181 caatttaatt ggcaaggtgc tgaagtccct catgctaaag gaggtatgag agctcatcct
      241 ccaaagataa ctcaatttgt aagagactta aaaataaata aaaaagaatt aaaaatagca
      301 ttattctctt caatttctgc aacagcctct gaaaaataca tattaaaaaa atattcaaca
      361 ataaagaaat tagacaaaaa agctccattt attgtttctt ctgatttaac taaaaagaaa
      421 acaaaagata ttttgagtga attgaaaaca attttaggaa atttatatcc tgtgtcaata
      481 aaagaaaaat caattagagc tggaaaagga aaattaagag gaagaaaata caaatcaaat
      541 gcaggaatga tattgataat tggtaaaaat gaaaatgtta aaactggttt atttgatgtt
      601 agaaaggcaa atgaattaag tgttagtgat ttagctaaag gagggttagg aagagtagct
      661 gtttatactg aagaagcagt aaaagaatta gaaaataaaa tggaaggtaa gaaaaaatga
      721 aactaaaacc aattgctaca gaaaaagcaa ttatgaaggt tgagagagat aatattattt
      781 cgtttttagt tgacgagaat tcaacaaagg cagaaataga aaaagaaata gaaaatttgt
      841 ttaaagtaaa agtagaaaag ataaggacaa gtgtaagaaa taatcaaaag atagcttatg
      901 ttaaattaaa taaaaaatat cttgcaatag accttgcaac taaattaggt ttaatgtaaa
      961 tgggaaaaag aataatacaa caggcacgtg ggcatggaag ttctacttac agagctaaac
     1021 ctactgcatt tgtttataaa ataagatatc ctaaaaaatt agaaggagag ggaacagttg
     1081 taaaaatatt acattctcct gctcactctg ctccaattgc taaagtttct tacagtggag
     1141 ggtcatttta tattcctgca ttttatggaa tgatagaagg acaaaaaata tcattcaatg
     1201 aaggaataaa acctggaaat atacttcctt tagaaaaaat accaattaaa acagaaattt
     1261 attgtattga atcaagacct ggagatgggg gaaaattcat aaaagcaggt ggaaatgttg
     1321 cggttgttac aagaataata ggagataaaa taatggttat gcttccatca aaaagagaaa
     1381 aaagttttaa tggaagatgt cgtgcagtga taggtaagat tgctgggcat ggaagattag
     1441 aaaaacctat tgtaaaggca ggtaaaaatt attatattaa aaaagctaga aataaattat
     1501 ggcctagaac aagtgcagta aagatgaatg caattgacca tcctttaggt tctggtagag
     1561 gaaagaacat aaagagtaag attgcaaaga gaaatgctcc tgcaggaagg aaagtaggat
     1621 taataaggcc aagaagaaca ggtaagaaga agtaaaatgg aaatacaaaa aaaacaattt
     1681 acatatcgtg gaaagactgt agatgaactt aaagagttgg aagtgcgtga gtttgcaaaa
     1741 tatttaccat ctagagaaaa aagaactgtg cttagaaatt tccagatgat agaggacttt
     1801 gttaagagag ctaaaactaa gattgagaaa ggaaagaaga taagaacaca ttcaagaaat
     1861 ataataattg tcccaaaaat gattggtatg acaatacacg tccataatgg aaagaatttc
     1921 aatcaagtgc atatagaacc tgaaatgtta gggcatagac ttggagaaat gtctggaaca
     1981 agatcgaagg ttgcacatag taaagcagga gttggagcaa caaaaggaag taaacacaaa
     2041 gctaagaaat aaataaaatg acagaaaaaa attataaccc aaatcaaaaa gaaaagaact
     2101 caatgaaaaa acagccaaaa actaagataa ttgaaaatcc tataaaaaca gaaaacaagg
     2161 cagaaacaga aaataaaaaa aatgaagata caactaaaga aaaaacacaa gttgtagaag
     2221 agaaaaaaac taaagaagat aaaaaagaaa ttaaaaaaca agtaaagaag ataaaaaaag
     2281 aagaagttta tgttaatgct aaaagtgttc caatatctac aaaatatgct gttaacttgt
     2341 gcaagtttgt taagggaaaa aggataggag atgctataag ggatttagaa caagtagtgt
     2401 tgttgaaaaa agctgttcct atgaaaggag aatattctca caaaaaagga aagggaatga
     2461 gtggaggagc ttatcctcaa aaagcagcaa aacattttat aatattgttg aaaagtcttg
     2521 caggaaacgc gaataatcat gaaataattg agccaataat agttgaggca agggcaaatt
     2581 gggctcctgc tccaagagga agatttggaa gagtgaaaag aaaaagaaca cacataacat
     2641 tgaaggcggt tgaaatgaaa attaaagaaa acaaaaagga gaataaaaag taaaaatgga
     2701 agaaaaacaa actgtgaaat tcaagaaaga agaatttgct attaaagaat cagttaaaaa
     2761 aatgcttgga aaaggtaaag ttagcaaagt tagaatagaa tatactcctg ttggtgaaaa
     2821 aataattatc tctactaata aacctggact catcataggt aagggtggag agaaattaaa
     2881 tgagataaca gaatatttaa gaaaacattt caaatttgaa aatttacact tagatgttga
     2941 agaaataaca gaaccagaat ttgatgctca aattgttgct gatgaaatag ctttaggatt
     3001 ggaaagattt ggacctttga aattcaaaat attagcatac aggagtttgg agaaagttat
     3061 gaaagcagga gcattagggt gtgaaataag gttaagtggt aaacttccta gttcaagggc
     3121 taaaacatgg agattcgctc agggatattt aaagaaaaca ggagatagtt caaaagtagt
     3181 tgacagagcg caatcaagag cagaaacaaa accaggaaca gttggaataa aagttagtat
     3241 actctcacct cgtgctgtat tgaaagacaa aatatttatc aacgaagaat taaaagaaaa
     3301 attaagaatg aatcaacaag aacttgaaga taaacctgtt aaaaaatcaa aaaggagtaa
     3361 gaaagattaa aaatgaaata caaggaaata aaaaatatga gcgatgaaga aagaaataag
     3421 aaattaaatg atttaaagat agaactttta aaatctagaa caaagtcatc aaaaacagga
     3481 agttcaaata caagagaaat aaaaaggaca attgcaaaga tactcacatt caacacctct
     3541 aaaaaaggta ttgaaaaaaa ataaactatg gaaatagatc caaagacagg attgccatta
     3601 caggcaatag aatgggaaaa cctcacgagg agttcgcaga gaataagtgt aacaacagat
     3661 aagaaaagat atggaaagat tgttacattg gtcagcgggt tcgacaagga tgtcgatata
     3721 aaaaaaatcg caaaggctct gaaaaatgag cttgcatgcg gcggcacctt caagaacaat
     3781 gtgatagaac ttcaaggaga ccatatgaag aagataaaac ctcttctcat aaagatgggc
     3841 ttcgagaaca actcaataga ggactaatcc cttcagaata aaaacaaaat ggaaagaaaa
     3901 caaaaaacaa aagctagaag taaggatact tctaagaaaa accaagaaga gaaaaagata
     3961 gctagtgctg aaaatgataa gaagattaca acaagaggaa gaatctttga aggcatagtt
     4021 actaaaaaat tcccaaaaag aatagttttg gaatttgaaa gaatggtgta tataaggaaa
     4081 tatgaaagat atgctaaaat gaaaataaag atgcatgcaa gattaccaga ttatattgaa
     4141 aaggaaatta atattggaga tttagtgaga attcgtgaat gtaggccact tagtaagata
     4201 atccactttg tagttataga aaaaataaaa gacgcttcgg aggcaaacaa atgaaagcaa
     4261 taagtgcaag aggaacaagt tcattaaatt taggcgcaga agttattgca gcagataata
     4321 gcggagctaa aatagtaaag atagtctcag ttaaaaaatc aaaggcaaaa aaaggaaagc
     4381 aagtttcggc taagatagct gattgggtta aggtatctgt aagaaaaggt gttcctgata
     4441 tgaaaggtaa agtatttgat gctgttgtta taagacaaaa gaaaccattc aggagattaa
     4501 gtggagaaag aattgcattt gaagataatg ctattgctat actaaaggat gaaaaaggaa
     4561 atccaaaagg aactcaaatt aaaggaccaa ttgcaaggga ggttcaggaa agatgggcac
     4621 aagttgccaa aatagcatct gtagtatatt aaaatgaaaa agaaattctc aacacaatgg
     4681 aaatcaagta aacaaccaag aaagaaaaga aaatacgtag ctaatgctcc attacaccta
     4741 agaaagaaat ttgtaagtat taacttatca aaagaattaa ggaaaaaaac aaataaaaga
     4801 aatattcctg caaaaaaagg agacaaagta aaaatttgtg taggaaaatt caagggaaaa
     4861 acaggaaaga tactaacagt taatcttaaa acatcaaaaa taatagttga agaaatccaa
     4921 gtaaagaaac aagatgggtc taaagttaat ttcaaacttc aaccttcaaa cctgcagatt
     4981 atagaacttg cagaaagaaa atcaaacaaa aaaaataaac aaaaagaaga aaaaaaagaa
     5041 tcttcaggaa ataaaaataa ggaaaaagga aaggagaata aaaaatgagt catataaaaa
     5101 gacaaattat acctaaaagt tggccaataa aaaggaaagg aactgctttt gtagttaaac
     5161 caacactcgg attttcaaac tctttaccaa tactaattat attgagggat ttgattaatg
     5221 ttgcacaaaa caggaaagaa gtgaaaaaga tgattaatat gaaaagtata ttgattaatg
     5281 gaagagaagt tcgtgatgaa aaagaaggag ttttgttatt tgatgtaata acgctagtgc
     5341 cttcaaaaaa acattataga cttagtatat ttaagaatgg aaaactaaat gtagaagaaa
     5401 taaaagagag tgaggcaaat acaaaaatat ctaagatagt aaataaaaaa attctaaagg
     5461 ggaagaaaac tcaattaaac ttaagtgatg gaagaaacct tctctcagaa ataaaatgca
     5521 atgttaatga ttctgttttg ataaacttaa aaacaaagaa aatagaaaaa tgtcttcctt
     5581 ttacggaaaa ttcagaagtg atagtatttt ctggaaaaca cgctggagag aaaggaaaga
     5641 ttgaaaaaat aaatccagaa agaaaaaatg cagaaataaa attagagaaa ggaaatataa
     5701 atgttttaat taaacaattg atggttataa attaagatga caaaagaaaa cattatgaga
     5761 aaaataaaaa tagagaaagt ggtacttagt gttagtggaa cagcagacgg gttagataaa
     5821 ggattcagat tattaaaatt cttaacagat aagaaacctg ctaaaataaa atctaaaaga
     5881 agaataccta cttggggagt taggcctgga cttgaggttg gggcagttgt tacactaagg
     5941 aaagattttg aacaattctt gaagagatta ttagtatctg ttgacaatac tctaaaaaga
     6001 aaacaaataa gtgaaaataa cttttcattt ggaataaaag aatatattga aatacctggt
     6061 gcagaatatc aaagagatat aggtataatt ggattagatg tcacagttgt atttaaaaga
     6121 agtggaagaa gagtaggact tagaaaaata aaaagaggga aagtctctaa aaaacaaaca
     6181 ataacaagag aagaaataat taaatttatg gaagataact tccaaacaaa attcatataa
     6241 aatgactgca agtgattgga aaaaaatact gaagcaatta gatgctaaac ctgtaattaa
     6301 agagaaatat atgaaacata atgtaccaaa gaaaagaaag attggaatag ctgctcaaaa
     6361 atgtgaaaga tgtgggagat ttggagcaca tataaaatca tatggattaa atttatgtcg
     6421 acactgcttt agagaaatag caagtgaaat cggctttaaa aaatacagtt aaaatgtcac
     6481 acgatgtagt tgcagacgca ttaaacatga taaggaatgc caaaaaggca ggaaaggaaa
     6541 atgttgagat aaagataatc tcaaatctac ttatagaaat attgaaaata atgaaacagg
     6601 aaaaagcaat aaagaaatat aaaatagatg caaaagaaaa gtcaataact cttacaattg
     6661 gggaactttc tgaatgtaaa gcagtaaaac caagattcag ttgtgataaa gaccaagttg
     6721 aaaaatacag aagaagatat ttacctgcta gaaatcttgg aacattaata atatctacaa
     6781 ataaaggatt aatgacacac aaagaagctc aagaagaagg aacaggagga tgtgtaatcg
     6841 catattttta ttaaaatgaa aaaagaattt tcacaagaaa tagaaattcc tgaaggaata
     6901 gaagtaaaga aagaagataa gaaaataatt gtaaaaggtc cagaaggaga agtttcaaaa
     6961 gaattcaact ttggtagaat aaaatttgaa aacaaagatg gaaaaatctt aatttcttat
     7021 cctatggcta caagaaacga taaaagaatg attaacacaa taactgcaca tttgaaaaat
     7081 atggttaaag gggtcaagga aaaatatgaa tataaactaa aaatatgctc tagccacttc
     7141 ccaataaatg tggagataaa aggaaatgag gcattaataa aaaacttcct tggagaaaaa
     7201 acacctagaa aatgcaaaat accccaagat gttgaagtta aaatcaataa agaaataata
     7261 acaataaaat ctgcgaataa agaacttgct ggtcaatcag ctgcaaattt tgaaatggcc
     7321 acagcagtaa gaaacagaga tagaagagta ttccaagatg gggtatacat aataaataaa
     7381 gacggagagg acatataaaa tgcctaaatt tttgagacat acatcgcaca aatattccaa
     7441 acttggattg agaagaaaaa agaaacaggt ttggagaaga cctactggaa gagataataa
     7501 gatgagagaa cagagaaaag gaaaacctgc aatagttgcc attggatatt caactaataa
     7561 aaaagaaaaa ggaaaaataa ataataaaac acctgtagtt gtaaataatg ttcgccaatt
     7621 agagaatata caaaaaaatc aaattgttgt attaggtaga ataggtaaaa aaaataaaat
     7681 agagatagca gaaaaagcaa atgaacttaa aatagaaatc tacaagttta accctcaaaa
     7741 atatttgaaa atgaataaga aaaaagataa aaaaattgaa aaaaaagaaa aggagaataa
     7801 aaaatgaatt taagatctaa aaaacaatta gctgcaaata cctttggagt tggaaaatca
     7861 agaataatgt ttgtaaatga aaggattgag gaaattaaag aagctataac aaaacaagat
     7921 atgagaaatt tgcttcaaga cggagctata ataatcaaag aagtaaaagg aagaagtaaa
     7981 aatgttggaa agaaaaagaa aaggaaattt tccaagagaa gaaaaaatgt aaatacaaga
     8041 aaaaaagatt atgtcatatt aacaagaaaa ttaagaaaat taatagcaga tagaaaacaa
     8101 aaaggagaaa taaataaaga agaagaaaag aaattaagaa ataggataag aaataaaatg
     8161 ttcaagagta aagcacattt aaaagaatat atgggagga
//
LOCUS       DAOPCH010000039         8059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_8219_length_8059_cov_22.590955, whole genome shotgun sequence.
ACCESSION   DAOPCH010000039 DAOPCH010000000
VERSION     DAOPCH010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8219_length_8059_cov_22.590955"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1070)
                     /locus_tag="PLU55_03610"
     CDS             complement(123..1070)
                     /locus_tag="PLU55_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87173.1"
                     /translation="MADIRVELHKKNASNGFDRLLVETDINSVEGLLNSDSVINTALL
                     PLWITGGMRYGGGIAFDNDNFPSQTTFESFWSLYNFTQFVSDAFYQIRGNYFIAPNTM
                     TVQMSNFQINAGGTIGPVDVDFNLTTVSADDILTSGNYTYLDLEAGDWMVISTTTFLE
                     KTEIDNAKLTLKIDVVNNTYPFASDTNHGVVKWATEIDVNNHNAVKASTLYAQLTGLI
                     TALHHESGVTAGSGNYFTVNAQGHVTAKEMRNASEAVSGFIKIATTAEMQVDTNDALA
                     MTPKKTKQAIDWWGGLKYYQALPASADVAEGCIVAVLKS"
     gene            complement(1070..1636)
                     /locus_tag="PLU55_03615"
     CDS             complement(1070..1636)
                     /locus_tag="PLU55_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87174.1"
                     /translation="MGKKYEELKKPELIALLVREQEHLAAAVESKDQEIAKLEETKNS
                     EIAKLKEELKLREKTIKEQNATIERQSHLASAVDAKDEEITKLTNDLVKTQDKINDLN
                     KKIIDLEVKIKTEDVEELKEENEQLKADIAFLERKFLSMTDIFAYNLKDQQALLERNN
                     FSRNEIWEDFNLYRQNKTQREKGKEVKK"
     gene            complement(1653..2174)
                     /locus_tag="PLU55_03620"
     CDS             complement(1653..2174)
                     /locus_tag="PLU55_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87175.1"
                     /translation="MIIRIDSNFNIIDKIDEPDDKLIIATEIGVKKLKFYYVNDSADY
                     VVNISFKRADNMVIGGFNVALVEDENGIKYREFILDIEELTAIAGALQITIRYEKYFD
                     NELIFSRPINMVVAIYEAVATGNDTLSFLIEELEQTKRELEDIKESGVAGGVIYIKQD
                     NSIIKAPLYIKEK"
     gene            complement(2190..2984)
                     /locus_tag="PLU55_03625"
     CDS             complement(2190..2984)
                     /locus_tag="PLU55_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87176.1"
                     /translation="MPNIVLDKEQHNYIVFNQYGKIKEKNLFDFPAGSSGIEIYILAD
                     FDNVEASEYETYANIKRPDGLIVGDVILEKLANQIDLNGNLYWGKKLTLYDDVTQLNG
                     AIQISIVYSKFDSNLRKIKQRINGMIVFHIYDAVSFYTPRSSVINAMMRYVDQQALGG
                     GLTYGAKALAIPINSPEPSVAPDIPATAQVVYITPIPKNKTTHSVLNTKIIPLSEIGS
                     GVSQAVKLGYDATDSRVYKGNGNTKLWVEWYEDGYADTHDLVIYFQ"
     gene            complement(2995..5460)
                     /locus_tag="PLU55_03630"
     CDS             complement(2995..5460)
                     /locus_tag="PLU55_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87177.1"
                     /translation="MVEIKILINGVEQANDRIKLESGFTFKETITDELENKIVIFLVS
                     HNNKNLLKVNDIIEIQREVEGTFRDKTPFLVWHLERELFTKMHPYYWKITAILVEPTK
                     ILERITLPPCAFTNSMNTLQQIFSKVLDKAIIKRGVNPQSKFNVSSNLASLIANTTGE
                     DFVYNKPVLLREVLDDLLLLKNARIKVLKKENGIIYLDYKLLSYENKAIKDINLLEHF
                     TLKGLKEIESANGIANTYETYVNEVITQSPIQDNWRVFKPKEADILTSQDYYYCFDKP
                     IEHMEKFEVLVKVKWTLSHSDPLQYDTMIVQRYEVLDLTDSFVEEDIYNLTNESDQRY
                     KIPYKRKEKYMYCLEDVGTVIGIKIENRTHNAFLARQVALMQDSSFMGEYDVLNYKII
                     DKNGNELETDIWNYQNFLFRAKYYAYMDQHYKFTKANNDLGTILVSPNESLIDSDRFA
                     DKMFNITKSQGNKRLEADILVANYFQDLNEILVGDRFWYENKLYTVIEREKGFYKEHV
                     KDHIVLEADYIPEIPEKLSRERQIFKNPIDKTITRDILVHDKVVFNLTNTNYGTNTLF
                     NLNQLTTLLKNSLIHRLEPPKLNGYLFANGVHFIYNITPVKVKNSVIYNFGFLDNYSA
                     GYTKDVSTLGGFKVGFNSYVDSNGEVENISIQFLSNNYDEPNYSTTDLADLMEHLENL
                     PKVRANDIADYYDYKSNNLLLKVQKDRLEHITFTYQLEFLSSEEVIIGPEFINVFNLV
                     GRSNSNFKIYASKTLDYRDGDRFAKGLEQFNATITDAGLIEITGLDEDVANYKSFAIC
                     IGNDRRLVLGFNNNFHDKKTLRY"
     gene            complement(5467..5895)
                     /locus_tag="PLU55_03635"
     CDS             complement(5467..5895)
                     /locus_tag="PLU55_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87178.1"
                     /translation="MANEKTYVIRLTKDGGSGAYEETSYSPQGNLSSQEGKINKKQST
                     TQNVAQMLAFKVGTDAVKYSLSNYGELTGDYVGQEAIEDITGIAGDIFMIAKGGWLGV
                     AAVGVKYATKIATTSIRLNKSKTRTSMMQERVGITTIKGW"
     gene            complement(5907..>6784)
                     /locus_tag="PLU55_03640"
     CDS             complement(5907..>6784)
                     /locus_tag="PLU55_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87179.1"
                     /translation="GCICVNFKKRYIEWLEQELSGYLPSGYDLKVIDESTQSPQIKPL
                     KTEIRVYVKFGSGSKQVNTQDRINQTLTITALSESDNFDKTYQVLYNLFTEYSKKITI
                     LENVEVLENDVYVASDYNVWHNYQTPMIMNPYQQIGTESRTSLIMTGVLSYSKGTIIG
                     SLHYIDDEIVDIIDPSGTYQMQQVAFNPINTPAAISFNESAIITFSGRFLLTNSNVSR
                     KFLDMNYYHMVMFEDGVTPYVWKPIYKVVYNPLTSSPIEYTLLCNIANIQIEKDSANN
                     DNIVSFVLTAIDIEN"
     assembly_gap    6785..6884
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6885..7253)
                     /locus_tag="PLU55_03645"
     CDS             complement(6885..7253)
                     /locus_tag="PLU55_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87180.1"
                     /translation="MAEINLMNEAKMIVGALLGAVPKDTGALASSIGFNVLDNNTIEI
                     LVGNEIVDYAVHTNIEWQPPINLEYFPSGRKRTETDKSRLKSFKFGGKNPNERWFDGA
                     LEQCLLALAEKYKGVYLREL"
     gene            complement(7256..7627)
                     /locus_tag="PLU55_03650"
     CDS             complement(7256..7627)
                     /locus_tag="PLU55_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87181.1"
                     /translation="MGRDARNTSSRYRIRCKVFETITNNEDRIARLTNSKPIVFYARV
                     IQDYKESYFFVDNYNTTLGNVVIETRDLKQNDIKRLDTVEYLGKLYNVENIVVKPEER
                     QIGITTRPLIKTIIALGKEVK"
     gene            complement(7637..>8059)
                     /locus_tag="PLU55_03655"
     CDS             complement(7637..>8059)
                     /locus_tag="PLU55_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87182.1"
                     /translation="ELITITREEFLTYSGRDLSIELPSNDRDTDKVVTTINLWTDRVY
                     DEIFVRFDKYDSENFSERQKQAIKRAICDYGMYYLTNGDLYRESGYSLENGSNVSQSE
                     LENMRFPNYIKENLRRIGLRIRNLSYGYNPHCQDKDCF"
BASE COUNT         2388 a         1510 c          883 g         3178 t
ORIGIN      
        1 gttgttggtt tgttgggaat accataccag tcagcatttt gtagcaaaac gctttcaaaa
       61 tcactaccag tttttttata taattgtatt ttaatatctg ccataaaatt atatctccaa
      121 tcttatgact ttaatacagc gacaatacaa ccctcagcta catcagcact tgctggtaat
      181 gcttgataat actttaatcc accccaccaa tctatagctt gttttgtttt ctttggtgtc
      241 atagctaaag catcatttgt atctacttgc atttcagctg ttgttgctat tttaataaac
      301 ccactaactg cttcacttgc attcctcatt tctttagctg taacatgtcc ttgtgcgttt
      361 actgtgaaat aattgccact tccggctgtt actccacttt catggtgtaa tgctgtaatt
      421 agtcctgtta attgtgcgta taatgtagat gctttaactg cattatgatt attcacatca
      481 atttcagtcg cccattttac tacaccatga tttgtatcac ttgcaaaagg atatgtattg
      541 tttactacat ctatttttaa tgttaattta gcattatcaa tttcagtctt ttctaaaaat
      601 gttgtagttg aaataaccat ccaatcgcca gcttctaaat ctaaataagt ataattacca
      661 cttgttagaa tatcatcagc acttactgtt gttaagttaa aatctacatc tactggtcct
      721 attgttccac ctgcattgat ttgaaaatta ctcatttgca ctgtcattgt atttggtgct
      781 atgaaataat tacctcttat ttggtaaaat gcatcactta caaattgcgt gaaattatat
      841 aagctccaaa aactttcaaa tgtcgtttgg cttgggaagt tatcattatc aaatgcaatt
      901 ccaccaccat atctcattcc accagtaatc caaagtggta ataatgctgt gttaataaca
      961 ctatcactat ttaataaccc ctctactgag ttaatatcag tttccacaag cagcctatca
     1021 aaaccattac ttgcattttt cttatgtaat tctactctaa tatcagccat tattttttaa
     1081 cctctttccc tttttctctt tgtgttttgt tttgcctata taaattaaaa tcttcccata
     1141 tttcatttct tgaaaaatta tttctttcta ataatgcttg ttggtctttt agattataag
     1201 caaatatatc agtcatagat aaaaacttcc tttctaaaaa tgctatatca gcttttaact
     1261 gttcgttttc ttcttttaat tcttcaacat cttcagtttt tatcttaact tctaaatcaa
     1321 taatcttttt gtttagatca ttgattttgt cttgtgtttt tactaaatca tttgtaagtt
     1381 tagtaatctc ttcatctttc gcatcaactg cacttgctaa atgcgactgc ctttcaattg
     1441 ttgcgttttg ttccttaatg gttttttctc gcaactttaa ttcctctttt aatttagcaa
     1501 tttcactgtt ttttgtctct tccaacttag ctatttcttg atccttactt tcaacagctg
     1561 ctgctaaatg ttcttgttcc cttaccagca atgctattag ctctggtttt tttaattcct
     1621 catacttttt tcccataaat acctcatttc ttttattttt cttttatgta aagtggagct
     1681 tttataatag aattatcctg tttaatataa ataactccac cagctacacc actttctttt
     1741 atatcttcaa gttctctttt agtttgctct aactcctcta ttaaaaaact taaagtatca
     1801 tttccagttg ctactgcctc atatattgca actaccatat ttatcggtct tgagaaaata
     1861 agttcattat caaaatactt ctcatacctt attgtgattt gtaatgctcc agctatagct
     1921 gttagctctt caatatcaag tatgaattct ctatacttaa taccattttc atcttctact
     1981 aatgctacat taaacccacc aataaccata ttgtctgctc tcttaaatga gatattaaca
     2041 acataatcag cactatcatt tacataataa aactttaatt tcttaacacc aatttcagta
     2101 gctataatta gcttatcatc tggctcatct attttgtcaa tgatattgaa attactatct
     2161 attcttataa tcataaaatc acctaatttt tattggaagt aaataaccag atcatgtgta
     2221 tcagcatagc catcttcata ccattcaacc caaagtttag tgtttccatt gcctttataa
     2281 actcttgagt cagtagcatc ataacctaat ttaactgctt gacttacacc acttcctatt
     2341 tcacttaaag gtataatttt cgtatttaat actgagtgag ttgttttatt ttttggaata
     2401 ggcgtgatat aaacaacttg agctgttgct ggaatatctg gtgctacact cggttctgga
     2461 ctatttattg gaattgctaa agctttagct ccataagtta atccaccacc taaagcttgt
     2521 tgatctacat atctcatcat agcattaatt acacttgatc tcggcgtgta aaagcttact
     2581 gcatcataga tatgaaagac tatcatacca ttaattcttt gtttaatctt tcttaaatta
     2641 ctgtcaaact tactataaac tatactgatt tgtatcgcac cattaagctg tgtcacatca
     2701 tcatataaag ttagtttctt tccccaatat agattaccat ttagatctat ttggttagct
     2761 aacttttcta aaataacatc acccacaatt aagccatctg gtcttttaat gttcgcataa
     2821 gtttcatatt cgcttgcttc tacattgtca aaatcagcta aaatataaat ctcaatacca
     2881 cttgagccag ctgggaaatc aaataagttc ttttccttaa tcttcccata ttgattaaat
     2941 acaatatagt tatgttgttc tttatctaat acaatatttg gcatataaac ccccttaata
     3001 ccttaatgtt ttcttatcgt gaaaattatt attaaatccc aatactaatc ttctatcatt
     3061 tccaatgcat attgcaaagc ttttataatt tgctacatct tcatctaaac cagtgatttc
     3121 tattaaacca gcatcagtta tagttgcgtt aaattgttct aaccctttcg caaatctatc
     3181 accatctcta taatctaatg tcttacttgc ataaatctta aaattactat ttgacctacc
     3241 tactaaatta aatacattga taaactctgg accaattatt acttcttcac tacttaaaaa
     3301 ctctaattga taagtaaaag taatgtgttc taacctgtct ttttgcactt ttaatagtaa
     3361 attattactc ttataatcat aataatcagc tatatcattt gctctaacct ttggtaagtt
     3421 ctctaaatgt tccattaaat cggctaaatc ggttgttgaa tagttaggtt catcatagtt
     3481 attacttaaa aattgtatag atatattttc tacctcgcca ttactatcta cataagagtt
     3541 aaaccctacc ttaaaaccac ctaatgttga aacatctttc gtataacctg cactataatt
     3601 atctaaaaac ccaaagttat agataacgct attttttacc tttactggtg ttatattata
     3661 aataaaatgc accccattag caaataaata accatttagc tttggtggtt ctaatctatg
     3721 aattaaacta ttctttaata gtgttgttag ttgatttaag ttaaataatg tattagttcc
     3781 ataattagta tttgttaggt taaacactac tttatcatgc actaatatat cacgtgtgat
     3841 tgttttatct attgggttct taaagatttg tctttccctt gataatttct ctggtatttc
     3901 tggaatataa tcagcttcta aaacaatatg atctttaaca tgttctttat aaaacccttt
     3961 ttctcgttca ataacagtat aaagtttatt ctcataccaa aatctatcac caactaaaat
     4021 ctcatttaag tcttgaaaat aattagccac taatatatca gcttctaatc tcttattacc
     4081 ttgacttttt gtgatattaa acattttatc tgcaaatcta tcactgtcaa ttaagctttc
     4141 atttggactt actaatatag ttcctaaatc attattagct ttagtaaact tataatgttg
     4201 gtccatatag gcatagtatt tagctctaaa taaaaagttt tgatagttcc agatatcagt
     4261 ttctaactca ttaccgtttt tatcgataat cttataattt aagacatcat attcccccat
     4321 aaatgagctg tcttgcatta aagctacttg ccttgctaaa aatgcattat gtgttctatt
     4381 ttctatcttt attccaatta cagttccaac atcttccaaa caatacatat atttttcttt
     4441 tctcttatat ggtattttat atctttggtc gctttcattt gttaagttat aaatatcttc
     4501 ttctacaaaa ctatcagtta aatctaatac ttcatacctt tgcactatca ttgtgtcata
     4561 ttgtaatgga tcactatgtg ataaagtcca ttttactttt actaacactt caaacttttc
     4621 catatgttcg ataggtttat caaaacaata ataataatct tgacttgtta atatatcagc
     4681 ttcttttggc ttaaacactc gccaattatc ttgaatagga ctttgcgtta ttacctcatt
     4741 tacataagtt tcataagtat ttgctatacc attagcactt tctatttctt taagcccttt
     4801 taatgtaaaa tgctctaata ggttaatatc tttaatagct ttattttcat aagataatag
     4861 cttgtaatct aaataaataa taccattttc tttttttaat acttttattc ttgcattttt
     4921 aagtaaaagt aaatcatcta atacttctct taacaatact ggtttattat aaacaaaatc
     4981 ttccccagtt gtatttgcta ttaaacttgc taaattactt gatacattaa acttactttg
     5041 agggttcaca cctcttttaa taatggcttt gtctaacact ttggaaaaga tttgttgaag
     5101 tgtgttcatg ctatttgtaa acgcacaagg aggcagtgtt attctttcta atatcttagt
     5161 aggttctacc aaaatagcag ttattttcca ataatatgga tgcatcttgg taaataactc
     5221 tctttctaaa tgccacacta aaaaaggtgt tttatctcta aatgttccct caacttctcg
     5281 ttgtatctct ataatatcat ttacttttaa caagttttta ttattatgac taactaaaaa
     5341 tataactatc ttgttctcta attcatctgt gatagtttcc ttaaatgtaa aaccactttc
     5401 taacttgatt ctatcgttag cttgttctac accatttatt agtattttaa tttctaccat
     5461 attaaactac caccctttta ttgttgttat tccaaccctc tcttgcatca ttgatgttct
     5521 tgttttgctc ttatttagtc ttattgaagt tgttgctatt ttagttgcat attttacacc
     5581 cactgcagct actccaagcc agccaccttt tgctatcata aatatatcgc cagctatacc
     5641 agttatatct tctattgcct cttgtcctac ataatcgcca gttagttccc cataatttga
     5701 taagctatat ttaactgcat cagttcctac cttaaatgct aacatttgag ctacattttg
     5761 cgttgttgat tgctttttat ttattttccc ctcttgagaa gataaattac cttgtggcga
     5821 ataactggtc tcttcataag caccagagcc accatctttt gttaatctta ttacatatgt
     5881 tttttcatta gccatattaa atacctctaa ttttcaatat cgatagctgt taaaacaaag
     5941 cttacaatgt tgtcattatt tgcactatct ttttctattt gtatattggc tatattgcat
     6001 aataaagtat attcaatagg acttgaagtt aaagggttat aaaccacttt ataaatcggt
     6061 ttccaaacat aaggtgtaac accatcttca aacataacca tatgataata attcatatct
     6121 aaaaactttc ttgaaacatt actattagtt agtaaaaacc taccactaaa agttatgatt
     6181 gcactttcat taaatgatat tgcagctggt gtattgattg ggttaaatgc cacttgttgc
     6241 atttgatatg ttccgcttgg atctataata tctacaatct catcatctat gtaatgtaag
     6301 ctaccaataa tagttccttt tgaatagctt aaaacaccag tcattattag acttgtcctt
     6361 gattcagttc ctatttgctg ataagggttc ataatcattg gtgtttgata gttatgccat
     6421 acattataat cacttgctac ataaacatca ttttccaaca cttctacatt ttctaatata
     6481 gttatttttt tagagtattc agtaaatagg ttatataaaa cttgatatgt cttatcaaag
     6541 ttatcacttt cacttaaagc tgtgattgtt aaagtctggt ttattctatc ttgtgtgttt
     6601 acttgtttac ttccactacc aaactttaca tacactctaa tttcggtttt aagaggtttg
     6661 atttgtgggc tttgtgttga ttcatctatt acctttaaat cataaccaga gggcaaataa
     6721 ccacttaatt cttgttctaa ccactcgata tatcgtttct taaagttcac gcaaatacac
     6781 ccccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttaaag ttcacgcaaa
     6901 tacaccccct tatacttttc agctaaagct aataaacatt gttctaatgc accatcaaac
     6961 cacctctcat ttggattttt tccaccaaat ttaaaacttt ttaaccttga tttgtcagtt
     7021 tcagttcttt tcctaccact tgggaaatac tctaaattga ttggtggttg ccactctata
     7081 ttagtatgca ctgcataatc tactatctca ttacctacca agatttctat tgtattatta
     7141 tctaatacat taaaacctat actgcttgct aaagctccag tatcttttgg cactgcacca
     7201 agtaatgcac ctacaatcat tttagcttca ttcataagat taatttctgc cacagttatt
     7261 ttacctcttt acctaaagct attattgttt taattaaagg tcttgttgta ataccgattt
     7321 gcctttcttc tggctttacg actatattct caacattata caacttgcct aaatactcta
     7381 ctgtatctaa tctcttaata tcattttgtt ttaaatctct tgtttcaata accacatttc
     7441 caagtgtagt attgtaatta tctacaaaga aataactttc cttgtaatct tgtataactc
     7501 ttgcataaaa aactattggc ttgctatttg taagtcttgc aattctatct tcattatttg
     7561 tgatagtttc aaaaacttta cacctaattc tatatcttga gcttgtattt cttgcatctc
     7621 tacccataat tacaccttaa aaacaatctt tatcttgaca atgtggatta tagccatagc
     7681 ttaagtttct tattcttaag ccaattcttc ttaagttttc ttttatgtag ttcggaaaac
     7741 gcatattttc aagctcactt tgagaaacat tagatccatt ttctaaacta taaccgctct
     7801 cacgatatag atcaccgttt gttaaataat acataccata gtcacaaata gcccttttaa
     7861 ttgcttgttt ttgcctttcg ctaaagtttt cactgtcata tttatcaaac ctaacaaaaa
     7921 tctcatcata aactctatca gtccatagat taattgttgt aactactttg tcggtatctc
     7981 tatcgtttga tggaagttca atacttaaat ctctaccaga ataagttaaa aactcttccc
     8041 ttgtaattgt gattaattc
//
LOCUS       DAOPCH010000040         7911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_8370_length_7911_cov_31.965249, whole genome shotgun sequence.
ACCESSION   DAOPCH010000040 DAOPCH010000000
VERSION     DAOPCH010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7911
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8370_length_7911_cov_31.965249"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1015)
                     /locus_tag="PLU55_03660"
     CDS             complement(116..1015)
                     /locus_tag="PLU55_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87183.1"
                     /translation="MANIIEHISKHLHNELDEMFVKESVTGILELLRPGGIQLDFVNA
                     KTVRVPNIAMSALSNYSRTTGYAAGDINLTWEAFTLTQDRGKQFTLDAMDNEESGGIA
                     VIHAMKVFERTQVIPEVDAYRLSTLAAKATGTGIATPGTIAANQVIAKFADAEKYFED
                     NEVPIDRIVYFISTEVARLIKTTTELKALVGTTTVENGDIKLKVNTFNDNIIIVVPPK
                     RFKTAYTFGNNYFIPTADAKDINILAVYDAASIPVKKHNPVRIFSPEVVQDKDAWKFN
                     YRIYHDIFVPAKKIEGVWLHAKA"
     gene            complement(1040..1630)
                     /locus_tag="PLU55_03665"
     CDS             complement(1040..1630)
                     /locus_tag="PLU55_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87184.1"
                     /translation="MAKKMKKYSELTEEEKAALSEEELAALKAEEEAEKKAKEDGGEP
                     AKTDETPEEKEAKEGEQEPAKTEEPKIEMITLPKTEYEELLKNKYAEGARKAEKSLQD
                     QIAALQVENQALKAREVAATLVNPLYVDDLIALTKGKGLEVNEANLKEVAAHQPAWQK
                     STEEILQGQARMGASGDNTPPALDERATFKKLFNEA"
     gene            complement(1784..3016)
                     /locus_tag="PLU55_03670"
     CDS             complement(1784..3016)
                     /locus_tag="PLU55_03670"
                     /inference="COORDINATES: protein motif:HMM:NF017919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87185.1"
                     /translation="MVGARKWVYLTDRLIKDWELLFNELNEHEIKALTNYTKTDEWYE
                     KQLKDLKNYKKTIDKVVKEHKEKIMTNSQNAVDESIAVMGGMLNDTADTNAEPYAIYY
                     EEMNEFNDNMVKSVVKFATDRHSFFINKINAENCATKILKQYIVGTDENMAIIGGAKI
                     PIKTTEENILDLIAKKNNLGFFVKYANGRKMGIRSYLEMNLRTTLQNIATQKLKDSTD
                     NLGIIFFLASSHADCADDHVDYQGKIYVKENWQRFIKPEKIAEIQDFITKKDIKTMEW
                     VIGKPVYFTTRPNCRHYFTPITIEQALKKSLKELHKELKTEKPMTSEDKELTKKRYAA
                     LQEQRRNERKIRENKEKLNHLETFKAKTKNDTADSEIMKVKNNIAYYQAKQRALINAN
                     RGVLVRDYKKEQLGIEKQ"
     gene            complement(3029..>3868)
                     /locus_tag="PLU55_03675"
     CDS             complement(3029..>3868)
                     /locus_tag="PLU55_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87186.1"
                     /translation="EKTKGLEKTSYECFNQFIAVPTIYKLDKTTGRGKGMFSSKYDLL
                     DDLDQNLSMSSTTTRRSAPVDYLPEELMEYDDEGNRKKFNTFERKVMVYKNDLNSATG
                     VNQAMIETKQPVLNFEQYSSQALEILHNILAGILSPATLGIDLARDNNATAQREKEKV
                     TMITRDDLIDFQTETIEKLCNLLLKVYFFNNNNIKQAKEDYAIFVNYPEYANPSFDSK
                     LAVLAPVFASGAMSAKQYVNELWGDSLTEEEKEEEIKKLEERQSAYNAPDEEPYDLFN
                     IEG"
     assembly_gap    3869..3968
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3969..4477)
                     /locus_tag="PLU55_03680"
     CDS             complement(<3969..4477)
                     /locus_tag="PLU55_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87187.1"
                     /translation="MSFESKILRNRHNNDYGFNIDQRYRQEEGAIWYEGKSENLKRFY
                     TQNLTNLPNPSADFFTANQSYFWKVVAAEPQTKTTHSGLPHAIIDTLISIIGKPEIKV
                     NKKVLNEQTKNYEIVEDLVLTDLLNEILKDNNFQTLMDQDQLPYMLAVGDGAFFINYD
                     KSISDFPMIV"
     gene            complement(4474..5985)
                     /locus_tag="PLU55_03685"
     CDS             complement(4474..5985)
                     /locus_tag="PLU55_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87188.1"
                     /translation="MEQQQAVTNETQAVITPFNFSEKSLDLLRTKSSFDICEGTARSS
                     KTTTLAFKFGLHVNSSSHMQFFIAGATKGVARRNVVDEKNGFLALFRGCAREGTNTRY
                     GSHLVFTDTLGREKIIYIFGFKDKARWQTVLGSTLGGGIIDEINIADVNFVNEVFRSL
                     IAVDGFWLGATLNPDNPDKEIYTNYINKSIPLKKWEHDIPKPILEDLANADEKIKGAI
                     YWHFNFNDNPIMTQDKVDLFKSLYPKDSFYYASKILGLRGVSEGVIFKMLDDSYLSKS
                     SEYIYRAQKVIMDDIEYLFKTDAYTKYVFGVDLGGNQEKKGTRITFTGFHRQFQQLDV
                     LGRKKLISEEAVALVVEICDFIEKWYKQVLTPIKIDAVYIDGYGAVNVLLPTIRKELV
                     NRGINLKVRLAIKYGKNSTKTTDKATDADRHSRLMTLLLLFNLRRIRFINNSEGRELI
                     RQLKTLVYNPKDNLPLDENQDAMDDYDSLCYTITPFITELNDNIMRDEERKRL"
     gene            complement(5960..6637)
                     /locus_tag="PLU55_03690"
     CDS             complement(5960..6637)
                     /locus_tag="PLU55_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPJ87189.1"
                     /translation="MSKNKKNNTSKAKTSTKNTFFKPPKLTEEQREELAVKKPNRTNN
                     RKNKYEYWLTKDGLLLLESWSRDGLDLQQIAKKMGIGPRTLYTWRTKYEPIGHAIKKT
                     QELANLEVENALFKRAIGYNEIVKEAMKVKQGSYEEIVYVEKVIHIPGEVSAQIFYLT
                     NRKPERWTNTQARKVQLAEPVSAALISITENLNRIEITPTKDDLVDLSKYRTKEENAD
                     GTTTSSN"
     gene            complement(7041..7472)
                     /locus_tag="PLU55_03695"
     CDS             complement(7041..7472)
                     /locus_tag="PLU55_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87190.1"
                     /translation="MEIITFFEKLDKDILILTQEVKLFQIKLDFIIDASAPQMPKDKI
                     FGEPKGNNFFTAEELYNEVISLQTTIDLLLKALAELKAMKDIIGKNLKDFIKTSNDLE
                     YLIIKNHYIEKKSLKKIAQDLGYSYQYIRKTHMEIKKKLGK"
     gene            complement(7482..>7911)
                     /locus_tag="PLU55_03700"
     CDS             complement(7482..>7911)
                     /locus_tag="PLU55_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPJ87191.1"
                     /translation="RRIIMNKVILIGRLTKDPELRKTSNGNSFVNFQLAVSRNYNNQQ
                     GNVETDFINCQVWGTQADNLARYMRKGSQIAVEGSIQINTANVNGKTEYYTKVLVNLI
                     EYLSKAENNNNAPSVSPYEFNQEHNANSGGVGITDEDIPF"
BASE COUNT         2243 a         1407 c          994 g         3167 t
ORIGIN      
        1 attcgtaaaa agataaaggg taagctttaa cacctaccct ttatgtagtg ttaataattt
       61 ttatatgtgt ttatgaaaaa ttattgaaaa aatgttaatt aaattaaagc tttaattatg
      121 cttttgcatg tagccataca ccttcaattt tctttgctgg gacaaagata tcgtgataaa
      181 ttctataatt aaacttccaa gcatctttat cttgtaccac ttcaggacta aatattctta
      241 ctggattatg ctttttaact ggaatagatg ccgcatcata aacagctaaa atgttaatgt
      301 ctttagcatc tgcagttggt ataaaatagt tattgccaaa agtataagct gtcttaaatc
      361 tttttggtgg cactacgata atgatattat cattaaaagt attaaccttt aatttaatat
      421 ctccattttc aactgtagtt gttccaacta aagcttttaa ttcagtagtt gttttaatta
      481 atcttgcaac ttcagttgag ataaaataaa caattctatc aattggcact tcgttatctt
      541 caaagtattt ttccgcatca gcaaactttg cgattacttg gttagctgca attgttcctg
      601 gtgttgcaat accagttcca gtagctttag ctgccaatgt tgatagtcta taagcgtcaa
      661 cttctgggat tacttgcgtt ctttcaaata ctttcattgc atgaattact gcaattcctc
      721 cagactcttc attatccatt gcatctaatg tgaattgttt tcctctatct tgagttagag
      781 taaatgcttc ccatgttaaa ttaatgtctc cagctgcata accagtcgtt cttgaatagt
      841 tacttaaagc tgacattgcg atatttggca ctctaactgt ctttgcattt acaaagtcta
      901 attgaatgcc acctggtctc aataactcca aaataccagt tactgattct tttacaaaca
      961 tttcatcaag ttcattgtgt aaatgttttg aaatgtgttc gataatgttt gccataagtt
     1021 aataatttcc tcctttttct tatgcctcgt taaataattt tttaaatgtt gctctctcat
     1081 caagtgctgg tggagtatta tcgccgcttg ctcccatcct tgcttgacct tgcaaaatct
     1141 cttctgtaga tttttgccaa gctggttgat gagctgctac ttcttttaag ttagcttcgt
     1201 ttacttccaa ccctttacct tttgttaaag caatcaaatc atctacatat agaggattta
     1261 ctaatgttgc tgccacctct cgagctttta atgcttgatt ttcaacttgc aaagctgcaa
     1321 tttggtcttg caaagacttt tcagctttac gtgctccctc agcatattta ttctttaata
     1381 gttcttcata ctcagttttt ggtagagtaa tcatttctat ttttggctct tcagttttag
     1441 ctggttcttg ttcgccctct tttgcttcct tttcctcagg agtttcatca gttttagctg
     1501 gttcaccccc atcttcttta gctttctttt cggcttcttc ttcagctttt aatgctgcaa
     1561 gttcttcctc acttaaagct gccttttctt cttcagtaag ttctgaatac tttttcattt
     1621 tttttgccat ttaatactcc ttttacaact aaccttgtta tttggcttta aggttcaagt
     1681 tgtctttgct tttttacaag aaacataact tgtattttta attgcttttt tatacgagaa
     1741 acataactcg aatataatca gtctttttat ggacttatta cctttattgt ttttcaatac
     1801 ctaattgttc tttcttataa tctctaacta atacacccct attagcattg attaaagctc
     1861 tttgttttgc ttgataataa gctatattgt ttttaacttt cataatctca ctatcagctg
     1921 tatcgttttt cgttttagct ttgaaagttt ctaaatgatt taatttttcc ttattttctc
     1981 ttattttgcg ttcatttctt cgttgctctt gtaaagctgc atatcttttt tttgttagtt
     2041 ctttatcttc actagtcatt ggtttttcag ttttaagctc tttatgtagt tcctttaacg
     2101 atttctttaa tgcttgttct atcgttattg gtgtgaaata atgcctacaa ttcggtcttg
     2161 ttgtaaaata aactggcttt cctataaccc attccatagt ctttatatct tttttagtta
     2221 taaagtcttg tatttcggct atcttttctg gttttataaa tctttgccag ttctctttaa
     2281 cataaatctt cccttgataa tctacatggt catcagcaca atcagcatga gaagatgcta
     2341 aaaagaatat aatgcctaaa ttatcggttg agtctttaag tttttgtgta gctatatttt
     2401 gtagtgttgt ccttaagttc atttcaagat aagatctaat tcccatcttt ctcccattag
     2461 catactttac aaagaaacct aaattatttt ttttagctat tagatccaat atattttctt
     2521 ctgttgtctt aatcggtatt tttgctccac caataatagc catattttca tcagttccaa
     2581 cgatatattg cttcaagatt ttagtggcac aattttcagc atttatttta tttataaaaa
     2641 agctatgtct atcagttgca aacttaacta ctgacttaac catattgtca ttaaactcat
     2701 tcatttcttc ataataaatt gcataaggtt cagcattagt atcagctgta tcattaagca
     2761 ttccccccat aactgcaata ctttcatcta ctgcattttg tgagttagtc attatttttt
     2821 ctttatgttc tttcacaacc ttatcaatag tttttttata attctttaag tctttaagct
     2881 gtttttcata ccactcatca gttttagtat aatttgttaa agctttaatc tcatgttcat
     2941 ttaactcgtt aaataataat tcccaatctt taatcagtct atcggttaaa taaacccatt
     3001 tccttgcacc taccataact tatcactact aaccttcaat gttgaataaa tcgtatggtt
     3061 cttcatctgg tgcattatat gccgattgtc tttcttctaa ctttttgatt tcttcttctt
     3121 tttcctcttc ggttaatgag tctccccaaa gctcgttaac atattgttta gcactcattg
     3181 caccacttgc gaatactggt gctaatactg caagcttact atcgaaactt ggattagcat
     3241 attctggata attgacaaag attgcataat cttctttagc ttgtttaatg ttgttattgt
     3301 tgaaaaaata aacttttaat aaaagattac ataatttttc aattgtttcg gtttgaaaat
     3361 caattaaatc atcacgagtt atcatcgtta ccttttcttt ttctctttga gctgttgcat
     3421 tattatctct tgctaaatca attcctaatg ttgcagggct taaaatacca gctaagatat
     3481 tatgtaaaat ctctaatgct tgacttgaat actgctcgaa gttcaatact ggttgttttg
     3541 tctcaatcat tgcttgattt acaccagttg cagagtttag atcattttta taaaccatta
     3601 cttttctttc aaaagtatta aactttttcc tgttcccctc atcatcatat tccattaatt
     3661 cttctggtaa ataatctact ggtgctgatc tacgagttgt tgttgagctc attgataagt
     3721 tttgatctaa atcatcaagt aagtcatatt tactgctaaa cattccctta cctctaccag
     3781 ttgtcttatc aagcttgtag attgttggca cagctataaa ttgattaaaa cactcataac
     3841 ttgttttttc taaacctttt gttttttcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnac tatcattgga aagtcactta ttgatttatc ataattgatg aaaaaagcac
     4021 catcaccaac tgctaacata tatggtaatt ggtcttgatc cattagagtt tgaaaattat
     4081 tatctttcaa gatttcatta agtaaatcag ttaaaactaa atcttctaca atctcatagt
     4141 ttttagtttg ttcgttcaat acttttttat ttactttgat ttctggtttc ccaataatgc
     4201 taattaaagt gtcaataatc gcatgaggta agccagaatg agtagttttt gtttgtggtt
     4261 cagctgcaac tactttccaa aaatagcttt gatttgctgt aaaaaaatca gcacttggat
     4321 taggcaaatt agtaagattt tgagtataaa acctttttaa gttttcactt ttgccctcat
     4381 accaaattgc accctcttct tgtctatatc gttggtctat attaaaccca taatcattat
     4441 tgtgtctatt gcgtaaaatc ttgctttcaa aactcataat cttttccttt cttcatctcg
     4501 cataatgtta tcgtttagct cagtaataaa tggtgttatc gtataacaca agctatcata
     4561 atcatccatt gcatcttgat tttcatctaa tggcaaattg tctttaggat tataaacaag
     4621 cgttttaagc tgtcttatta gttctctacc ctcactgtta ttgataaatc ttattcttct
     4681 taaattgaat aataacaata atgtcatcag tcttgagtgt ctatcagcat cagttgcttt
     4741 atcagttgtt ttagttgagt ttttgccata tttaatagct aatcgcacct ttaagtttat
     4801 gcctctatta actaattctt ttctaattgt tggcagtaaa acattaactg caccgtagcc
     4861 atcaatgtaa actgcatcta ttttaattgg tgttagcact tgcttatacc atttctctat
     4921 aaaatcgcat atttcaacaa ctaaagctac tgcttcttca ctgattaatt tttttcttcc
     4981 taaaacatct agctgttgaa actgcctatg aaaaccagta aatgttattc gtgttccttt
     5041 tttttcttgg tttcccccta agtccactcc aaatacatat ttagtataag catcagtctt
     5101 gaatagatat tcaatatcat ccattatgac tttttgagct cgataaatat attcgcttga
     5161 cttacttaaa taactatcat caagcatttt aaagattact ccctcactta caccacgcaa
     5221 gccaagtatt ttacttgcat aataaaagct atctttaggg taaagtgatt taaacaaatc
     5281 tactttatct tgagtcatta ttgggttatc attaaaatta aaatgccagt atatagctcc
     5341 tttaattttt tcatcagcat ttgctaaatc ttctaaaatt ggttttggta tatcatgttc
     5401 ccatttctta agcggaattg atttgttgat ataattcgta tagatctctt tatctggatt
     5461 gtctgggtta agtgttgcac caagccaaaa accatcaact gcaattaaag atctaaatac
     5521 ttcattaaca aaattaacat cagctatatt aatctcatca ataataccac cgccaagtgt
     5581 agatcctaat actgtttgcc atcttgcttt atccttaaaa ccaaagatat aaattatctt
     5641 ttctcggcca agagtatcag taaagactaa atgactacca tatcttgtat tagttccctc
     5701 tctcgcacaa cctctaaata atgctaaaaa accatttttc tcatctacta catttcgcct
     5761 tgctacacct ttagttgctc cagctataaa aaactgcata tgacttgagc tatttacatg
     5821 cagcccaaac ttaaatgcaa gtgttgttgt tttactagat cttgctgttc cctcacaaat
     5881 atcaaagctt gatttagttc ttaataaatc taaactcttt tcactaaaat taaatggtgt
     5941 aatgacagct tgagtttcat tagttactgc ttgttgttgt tccatctgca ttttcctctt
     6001 tagttctata tttagataaa tctactaagt catcttttgt tggtgttatc tctattcggt
     6061 ttaagttctc agtaatagag attaaagctg cacttactgg ctcagccaat tgcactttac
     6121 gagcttgtgt attagtccat ctttctggct tacgattagt taagtaaaag atttgagctg
     6181 atacttcgcc aggaatatga ataacttttt caacataaac tatttcttca tagcttcctt
     6241 gtttaacttt catagcttct ttaacaattt cgttataccc aatagctctt ttaaacaaag
     6301 cattttcaac ctctaagtta gctaactctt gggttttttt tatagcgtgc ccaattggct
     6361 cgtatttagt tctccaagta tataaagttc taggaccaat acccattttc tttgctattt
     6421 gttgtaaatc taaaccatct ctactccaag actctaaaag caacaagcca tctttagtaa
     6481 gccaatactc atatttattt ttacgattat tagttctatt aggtttctta acagctaatt
     6541 cttctctttg ttcttcagtt aatttaggtg gtttaaaaaa tgtattttta gtagaagttt
     6601 tagctttaga ggtattattc tttttatttt tactcaaact aaaaccccct ttacttaatt
     6661 aaattattag tttatttttt gtttaagtaa actaaatata taaaaaatat acttaagtat
     6721 atgtaactta taaaataaaa aaaggaataa acttaatctt aaagcttatt cctctttcct
     6781 ctttttttaa tcaaataaga attaaaaaat gattaaagaa attaaaacac aagtgtaagt
     6841 aaataaagta agttaatatt taaagtaggt tctaaacata tgaaaagtaa tgatatgaaa
     6901 acactaaaga agacattaga aagtgtttat tttttatagt tttacttatt tacatactta
     6961 catacttaca cacctacatt ttatcacact tttcaaaaaa taaatgttgc ttttttgttg
     7021 ctaaaatgtt gctttttgtg ttattttccc aattttttct ttatttccat atgtgttttg
     7081 cgaatatact gatatgagta tcctaaatct tgagctattt ttttaagtga ttttttttca
     7141 atataatgat ttttaatgat taaatactca agatcattac ttgttttgat aaaatctttt
     7201 aggtttttcc caataatatc tttcattgct tttagttccg ccagagcttt cagcaataaa
     7261 tctattgttg tttgtaggct gataacttca ttgtaaagtt cttcagctgt aaagaaatta
     7321 tttcctttcg gttctccaaa gattttatcc tttggcattt gtggagctga tgcatcaatg
     7381 ataaaatcta actttatttg aaagagtttt acctcttgcg ttaaaattaa aatatcttta
     7441 tcgagctttt caaagaaagt gattatttcc atatacccct cctaaaaagg tatatcttca
     7501 tcagttatac cgacaccacc actatttgca ttatgttctt gattaaactc gtaaggactt
     7561 acacttggag cattattgtt gttttcagct ttagataaat actcaattaa attaactaaa
     7621 actttagtgt aatactcggt cttaccattt acattagctg tgttaatttg gatgctaccc
     7681 tctactgcaa tttggctacc tttacgcatg tatcttgcta aattatcggc ttgagttccc
     7741 cacacttgac aatttataaa atcagtttcc acatttcctt gttggttatt atagttcctt
     7801 gaaactgcaa gctggaagtt tacaaagcta ttaccattac ttgttttcct taattctgga
     7861 tctttggtta atctaccaat taaaattact ttattcatta ttattctcct t
//
LOCUS       DAOPCH010000041         7670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_8663_length_7670_cov_5.245174, whole genome shotgun sequence.
ACCESSION   DAOPCH010000041 DAOPCH010000000
VERSION     DAOPCH010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8663_length_7670_cov_5.245174"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLU55_03705"
     CDS             complement(<1..375)
                     /locus_tag="PLU55_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87192.1"
                     /translation="MVRLLQSRKRVFWEALILTIVVFLFGLLIGIFYESTKSSDMNEY
                     YVNSELSLMDIFALNSLLSINSQNCDVLTSSNLEFADKIYQEAVILEKYESVGKVTEH
                     MEVVHKRYDVLRTFLWINTIKTS"
     gene            complement(369..1598)
                     /locus_tag="PLU55_03710"
     CDS             complement(369..1598)
                     /locus_tag="PLU55_03710"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPJ87193.1"
                     /translation="MDTITVIYLIYIFIGFYFLFLFILIYIQNKKYLYYYPKPKRDYS
                     LSIVIPCYNEGATIEKTVKSVLDSGYKNLKKVIVVDDCSKDNSYKIIKELEKKYPKVL
                     AVQTPKNTGCAAGSKNYGAKFVDTELIGFSDGDSFIEKGSIDKLVGFFNDEKMGSVTC
                     SVLVANRDKFIERVQSVEYKVIKFSRKLLEFIDSIYVTPGPLGVFRKSAFDKIGGFDE
                     KNLTEDIEITWHLQAEGYKVRMALLSRAYTVAPSTIKDWVKQRNRWNIGGLQTSIKYG
                     RDWFRKGMLGKFILPFFVFSWVIGLFGLTVLAYRILKVLIFRLFSTTYSIQTQTSLIR
                     FEDISLMPNILIFFGLVMFFLSMWFTLLSLFHMKDENRKGENFFVLSFYMVFYVMIYP
                     IILISSFYKFLKGHRKW"
     gene            complement(1660..2280)
                     /locus_tag="PLU55_03715"
     CDS             complement(1660..2280)
                     /locus_tag="PLU55_03715"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPJ87194.1"
                     /translation="MPTSKTIEEIDEAKEKEDLKTLEAVFFISGRFLSMQDLISLSDL
                     NPIILTDLIERLKDKYNREDSALEIVEKSGMWKMDVRQEYSHIINKLATGSSEFTKAE
                     QGTLAIIAYKHPIKQSVIIKIRGNKAYDHIKKFSDLGLIKKKKTGHTHELSLSEDFYD
                     YFSISESERNTSNVLNEKINMEIEEAEKEVEKDESEGRFEDEKDNS"
     gene            2352..2876
                     /locus_tag="PLU55_03720"
     CDS             2352..2876
                     /locus_tag="PLU55_03720"
                     /inference="COORDINATES: protein motif:HMM:NF021601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HPJ87195.1"
                     /translation="MKKGVKLKKIVKVIIFSIIIVISLLFLIRLINPTEIDDVTPGIP
                     CPEIEKYNPDILYVIPNYNSLEISKDKEWCNQILSLNKSLSLHGLNHTYREFLYENIS
                     QEELNKGIREFEECFNFSPTSFKPPQLKINSQNKELILNNNLKLMGIYNQITHKVYHC
                     NDSDKIPNKIIRIF"
     gene            complement(2903..3991)
                     /gene="prf1"
                     /locus_tag="PLU55_03725"
     CDS             complement(2903..3991)
                     /gene="prf1"
                     /locus_tag="PLU55_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010867702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor aRF-1"
                     /protein_id="HPJ87196.1"
                     /translation="MAAISKAELEVLLEELEKHKGRHTELITVYISAGYDIVSVQKQL
                     EAEKSTAKNIKSTATRKNVVDALDKIVRYLKDFKGNPENGLAIFCGNVSKVEGMDDLQ
                     LWGIEPPLPLKTRLYRCDKDFVLEPLRELLEVSEIFALLVMDRKEATIGVLEGKRIEV
                     LQKMTSGVPSKVRAGGQSSQRFHRITEGLTKEFYKRIADEMKNIFYENKKLKGILIGG
                     PIPTKDEFIDNEYLTTVLRNKIIGRVDIGGSDESGLKELVEKSKDILANQEIVYEKKL
                     MEKFFEMLGAKPDFVAYKEEEVKKALEYGAVDTLFLSKEYEKESAKELKDIANNSGTK
                     IETISTDTEEGKQFLNLSGVGAILRYKI"
     gene            complement(4040..6415)
                     /locus_tag="PLU55_03730"
     CDS             complement(4040..6415)
                     /locus_tag="PLU55_03730"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPJ87197.1"
                     /translation="MSKPNSSSNPKFDFKDIESKWKSFWEKEKIYTVDLKKKEIYSVD
                     TPPPYISGKMHIGHAFSYSHQDFIIRFQRMLLADKNGSVFYPFGTDDNGLPTERFIEK
                     TNNMKSKDMGRTDFINLCLKTLKEITPDCVQDFKNLGISADYNVYYSTIDDNSRKISQ
                     KSFIDLYKSGEAYQKEFPTMWCPECQTSIAQAELEDKEEKSLFSTLKFSSGKKELLIA
                     TTRPELLGACVAVFVHPSDKKYSSFVGKKAKVPLFNYEVPIIADTSADPDKGTGALMV
                     CSYGDKYDVDAINRYNLVPKIIFDKSGRVNFGEYKGLKIKEMRKKILEDLKKENLIFE
                     QKEITHVVNCHDKCGHGIEFIPTKQWFIKILDKKEKFIEQGNKIKWYPEFMHKRYDNW
                     VNGLEWDWSISRNRHFGVPIPAWECKKCGQIIVAEERELPVDPLQLKKKCPKCKLEAN
                     PEKMVFDTWATSSMTPQITSSLIKNKITIPYSLRPQGHDIIRTWAFYTIVKSYLHSNT
                     IPWKDIVISGNVSLGGEKMSKSKGNVVSPIDVMNKYGSDALRYWAAGSKLGSDVDYLE
                     KDLVAGTKFINKLINASRFVFMNLKDYNPKNKPSKLEKIDEIFLDKLNLLIKDSTKNF
                     KEYEYSKAKLDTEKFFWSDFCDNYLEIVKKRVYQGEGNSKLSAQYTLYYSLLKILKLI
                     APIMPFITEEIYQEYFRKNEKEKSIHISPWPESDKKENSNHIKILEKLYSIISQIRHE
                     KSFNKKAMNVEIILTLEKEDYEEIKSVLEDLKNVSNAKVIREGKKFEVEFL"
     gene            6467..6628
                     /locus_tag="PLU55_03735"
     CDS             6467..6628
                     /locus_tag="PLU55_03735"
                     /inference="COORDINATES: protein motif:HMM:NF015846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HPJ87198.1"
                     /translation="MASNNKISLPGGFGGLMRYSEEYNSYINLKPIHVVLFVISLVAF
                     RILMPLIFK"
     gene            6671..6979
                     /locus_tag="PLU55_03740"
     CDS             6671..6979
                     /locus_tag="PLU55_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013683956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HPJ87199.1"
                     /translation="MQGMLKKMGISQEEIDASRVIIEKTDNSRIIIDNPSVTKIKMQG
                     QETFQIAGDISEESAKEETSEKDIKMIIEKTGVSEEIARETLEKNNGDLAETILELSK
                     "
     gene            6981..7184
                     /locus_tag="PLU55_03745"
     CDS             6981..7184
                     /locus_tag="PLU55_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87200.1"
                     /translation="MSFEPLYKKIIKKLEEEERVKTLSLQQTYSIDNQFAEGLSPIKQ
                     EFHKKRNASRYYINELERTTTQV"
     gene            7208..7402
                     /locus_tag="PLU55_03750"
     CDS             7208..7402
                     /locus_tag="PLU55_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87201.1"
                     /translation="MSTLINKTQEITEELKKQNNIQDVDYKILEEIDRIMKDIHQDYI
                     IKSYHSWIESENCYVYQNLN"
     gene            7404..7655
                     /locus_tag="PLU55_03755"
     CDS             7404..7655
                     /locus_tag="PLU55_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87202.1"
                     /translation="MSRLINPVERMLKKAIKNGEVKLLDETEYNDIWDKVEQDMEDYK
                     IMDNYRKGISLIEPNEILIYRNFNKFYKNFHPSRTAKDI"
BASE COUNT         2787 a         1293 c          849 g         2741 t
ORIGIN      
        1 agatgttttt atagtgttta tccataaaaa agttcttaac acatcatacc ttttatgaac
       61 tacttccata tgttcagtaa cttttcccac agattcgtat ttctcaagta taactgcttc
      121 ttgataaatt ttatctgcaa attccaaatt tgaagaagtt agaacatcac aattctgaga
      181 attgattgat aaaagagagt ttaaagcaaa tatatccatc aatgaaagtt cagaattaac
      241 ataatattca ttcatatctg aagactttgt tgattcataa aaaataccta ttaaaagtcc
      301 aaacaaaaaa acaactatag ttaaaattaa agcctcccaa aatactctct tcctgctttg
      361 caataatctt accattttct atgccctttc aaaaatttat aaaatgaact tattaaaatt
      421 atcggatata tcataacata aaataccata taaaaactta aaacaaaaaa gttttctccc
      481 ttacggtttt catctttcat atgaaataat gaaagcaaag taaaccacat gcttaaaaag
      541 aacataacta aaccaaagaa aataagaata tttggcatca aacttatatc ttcaaatcta
      601 ataagagagg tttgtgtttg aatagaataa gttgttgaaa acaatctaaa tataagaact
      661 ttcaatattc tataagctaa cacagtcaat ccaaataaac caattaccca agagaaaaca
      721 aaaaaaggaa gaataaattt accaagcatt ccttttctaa accaatctct accatacttg
      781 atacttgtct gaagaccccc gatattccat ctatttcttt gtttaaccca atctttaatt
      841 gttgaaggtg caacagtata tgctcttgat agtaaagcca tcctaacctt atacccttct
      901 gcttgaagat gccaagttat ttctatatct tctgtaagat ttttctcatc aaaccctcct
      961 attttatcaa atgcactttt tctaaataca ccaagaggtc caggagtaac ataaattgaa
     1021 tctataaatt ctaaaagttt tctggaaaat tttataacct tatattcaac agactgaact
     1081 ctttctataa atttatctct atttgctaca agaacagaac aagtaacact acccattttt
     1141 tcatcattaa aaaatcccac taatttatca atagaacctt tctcaataaa agaatcccca
     1201 tcactaaaac ctattagctc tgtatctaca aatttagctc cataattttt tgaacctgct
     1261 gcacaaccag tatttttagg ggtttgaact gctaaaacct taggatactt tttttcaagt
     1321 tcttttatta tcttataaga attatcttta gagcaatcgt ctacaacaat aactttttta
     1381 agatttttat atccagaatc taaaacagat ttaactgtct tctctattgt tgctccttca
     1441 ttataacaag gtatgactat actaagagaa taatctcttt taggtttagg ataataataa
     1501 agatattttt tattctgaat ataaattaaa ataaatagga aaagaaaata aaatcctata
     1561 aatatataga ttaaatatat tacagtaatt gtatccataa atgtaaatat aaaatttatt
     1621 cttttaagta ttgttgtgat aaatttgctc cttaattaat taagaattat ctttttcatc
     1681 ttcaaatctt ccttcagatt catctttttc aacttctttc tctgcttctt ctatttccat
     1741 attaattttt tcatttaaaa cattagaagt atttctttca ctctcagaaa tactaaaata
     1801 atcataaaaa tcttcactta aactcaattc atgtgtgtgc cctgtttttt tcttttttat
     1861 caaacctaaa tcagaaaatt tctttatatg gtcataggct ttatttcctc taattttaat
     1921 tataactgat tgttttatag gatgtttata tgcaattata gccaatgttc cttgctctgc
     1981 ttttgtaaat tctgaacttc ctgtagccaa cttatttatt atatgagaat attcttgtct
     2041 aacatccatt ttccacatcc cacttttctc aacaatctcc aatgctgaat cctcccgatt
     2101 atacttatct tttaatcttt caattaaatc agttaaaatt atcggattca aatcacttaa
     2161 agatattaaa tcctgcatac taagaaatct tccagaaata aaaaaaactg cttctaaagt
     2221 tttcaagtcc tctttttctt tagcttcatc tatctcctct attgtcttac ttgtaggcat
     2281 aaatgataaa aacaaattgt atttaaaagt attatcaata attatttaat agcaatttta
     2341 ataaaagtag tatgaaaaag ggggtaaaat taaaaaaaat agtaaaagta atcatatttt
     2401 caataataat tgtaatatcc cttttatttt taatcagatt aataaacccc acagaaatag
     2461 atgatgtaac tcctggaata ccttgtccag aaatagaaaa atacaatcca gatatacttt
     2521 atgtgattcc aaattataat agcttagaga tatcaaaaga taaagaatgg tgcaatcaaa
     2581 tcctatcttt aaataaatct ctttcactcc atggattaaa tcacacatat cgggaatttt
     2641 tatatgaaaa tatttcacaa gaagaattaa ataaaggaat aagagaattt gaagaatgtt
     2701 ttaatttttc accaacctca tttaaaccac cccagttaaa aataaactct cagaacaaag
     2761 aactaattct aaacaataat cttaagttaa tgggaattta caatcaaata actcataagg
     2821 tttatcattg caatgattca gataaaatac ctaacaaaat tataagaata ttttaaaaaa
     2881 gattaattta ggataaatta aattatattt tatatcttaa tatagcccca acacctgata
     2941 gattcaaaaa ttgttttcct tcttctgtat ctgttgaaat tgtttctatt tttgtaccac
     3001 tattatttgc aatatccttt aattcttttg ctgactcttt ctcatactct ttagataaaa
     3061 acaaagtatc tactgcccca tattcaagag ctttttttac ttcttcttcc ttatatgcta
     3121 caaaatctgg ttttgctccc aacatttcaa agaatttttc cattaatttc ttttcataaa
     3181 ctatctcctg atttgcaagg atatcttttg atttttcaac aagttcttta agtccagact
     3241 catcacttcc cccaatatcc actctcccaa taattttatt tcttaaaaca gtagttagat
     3301 attcattatc aataaattca tctttagtag gaataggacc gcctattaaa atacctttta
     3361 attttttatt ctcataaaag atatttttca tttcatctgc aattcttttg taaaattctt
     3421 tagttaatcc ttctgtaatt ctgtgaaatc tctgggaact ctgtcctcct gcacgaactt
     3481 tacttggaac accagaagtc atcttttgta aaacttctat tcttttaccc tcaagaaccc
     3541 ctattgttgc ttcttttcta tccataacta ataatgcaaa tatctctgaa acctctaata
     3601 actctctaag gggttcaagc acaaaatctt tatcacatct atataatctt gtctttaaag
     3661 gcaaaggagg ttcaattccc cacagttgta aatcatccat gccttctact tttgaaacat
     3721 taccacaaaa aatagcaagc ccattttcag gatttccttt aaaatccttc aaatacctca
     3781 caattttatc aagagcatca acaacatttt ttcttgttgc agtagacttt atgtttttag
     3841 cagtagattt ttctgcttcc agctgtttct gaacagaaac aatatcatat cctgctgaaa
     3901 tataaactgt aatcaattca gtatgtcttc ctttatgttt ctctaattct tcaagaagaa
     3961 cttcaagttc tgctttgctt attgctgcca tgtttaaaga aaatgaaatg ggtttataaa
     4021 attagccaat caacaaaagt tacaaaaatt caacttcaaa tttttttcct tctcttatta
     4081 ctttagcgtt tgaaacattc tttaaatctt caagaacaga ttttatctct tcataatctt
     4141 ctttttccaa agtaagaata atctctacat tcattgcttt tttattaaaa gatttttcat
     4201 gtctaatctg tgaaataata gaatagagtt tttccagtat ctttatgtga ttagaattct
     4261 cttttttatc tgattctggc caaggactaa tatgtattga tttttctttt tcattcttcc
     4321 taaagtattc ctgataaatt tcttctgtga taaaaggcat aattggtgca ataagcttca
     4381 aaattttaag taaagaataa tataatgtgt attgtgcaga taattttgaa ttaccctctc
     4441 cctgataaac tcttttctta acaatctcaa gataattatc acaaaaatca ctccaaaaaa
     4501 acttttctgt atcaagttta gctttagaat attcatactc tttgaaattc ttagtagaat
     4561 ctttaatcaa aagattaagt ttatctaaaa agatttcatc tatcttctct aacttagaag
     4621 gtttattttt aggattataa tctttaagat tcataaaaac aaatcttgaa gcatttatca
     4681 atttatttat aaattttgta cctgcaacta aatctttttc aagataatct acatcacttc
     4741 ccagttttga acctgcagcc caatatctca atgcatctga accatattta ttcatcacat
     4801 ctattggaga aacaacattc ccttttgact tagacatttt ttctcctcct aaagaaacat
     4861 taccagaaat aacaatatct ttccaaggaa tagtattaga atgtaaataa gatttaacta
     4921 ttgtataaaa tgcccaagtt cttattatat catgtccttg tggtcttaaa gaatatggaa
     4981 tagtaatttt atttttaata agagagcttg ttatttgagg agtcatagaa gaagttgccc
     5041 aagtatcaaa taccattttt tctggatttg cttctaattt acatttagga catttctttt
     5101 ttaattgaag agggtccaca ggaagttctc gctcttcagc aacaattatt tgtccacatt
     5161 ttttacattc ccaagcagga ataggaactc caaaatgcct atttcttgaa atagaccagt
     5221 cccactctaa accattaacc caattatcat atcttttatg cataaattca ggataccatt
     5281 taatcttatt tccttgttca ataaattttt cttttttatc taatattttt ataaaccatt
     5341 gtttagttgg aataaattct attccatgtc cacacttatc atggcaatta actacatgag
     5401 ttatctcttt ttgttcaaaa attaagtttt ctttttttaa atcttcaaga attttctttc
     5461 tcatttcttt aattttcaaa cctttatatt ctccaaaatt aactcttcct gatttgtcaa
     5521 aaattatctt tggaacaaga ttatatctat ttattgcatc aacatcatat ttatctccat
     5581 aagaacaaac cattaatgct cctgttcctt tatcagggtc tgctgatgta tctgcaatta
     5641 ttggaacttc ataattaaat aatggaactt ttgctttttt tccaacaaaa ctagaatatt
     5701 ttttatctga aggatgaaca aaaacagcaa cacatgcacc taaaagttct ggtcttgttg
     5761 ttgcaataag tagctctttt tttccagatg aaaactttaa agttgaaaac aaacttttct
     5821 cttctttatc ttcaagttca gcctgagcta ttgaggtctg gcattcagga caccacattg
     5881 taggaaattc tttttgataa gcttcccctg acttatataa atcaataaaa gatttttgag
     5941 atatttttct ggaattatca tcgattgtag aataatatac attataatct gcagatattc
     6001 ctaaattttt aaaatcctga acacaatcag gagttatttc ttttaatgtt ttaagacaaa
     6061 gattaataaa atctgttctt cccatatctt tgcttttcat attatttgtt ttttctatga
     6121 acctttcagt aggcaatcca ttgtcatctg tcccaaatgg ataaaaaaca ctcccattct
     6181 tatctgctaa taacattctc tgaaacctta taataaagtc ttgatgagaa taagaaaatg
     6241 catgcccaat atgcatctta ccagaaatat atggaggagg tgtatcaact gaataaatct
     6301 ctttcttttt taaatccaca gtgtaaatct tttctttttc ccaaaagctc ttccacttac
     6361 tttcaatatc cttaaagtca aattttgggt ttgaactcga atttggtttt gacataaata
     6421 atcttatcca agaaactttt aaaaactatc tctctttaaa atatccatgg catcaaacaa
     6481 taaaataagc cttccaggag gatttggggg tttaatgcgt tacagtgaag aatataatag
     6541 ttatattaat ttaaaaccta ttcacgtagt cctcttcgta atatcacttg tagcatttag
     6601 aatactaatg cccctcatat tcaaataaat ttaattttct aaaatgtttc caggagttaa
     6661 tcccagacaa atgcaaggaa tgcttaagaa gatgggtatt tcccaagaag aaatagatgc
     6721 ttcaagagtt ataattgaaa aaacagataa ttcaagaata ataattgata acccttctgt
     6781 tacaaaaata aaaatgcaag gacaggaaac attccaaatt gcaggagata tttcagaaga
     6841 gtcagcaaaa gaagaaacct ctgaaaaaga tataaaaatg ataatagaaa agacaggtgt
     6901 ttcagaagaa attgcaagag aaacacttga aaaaaacaat ggagatttag ctgaaacaat
     6961 tttagaatta agtaagtaaa atgtcatttg aaccattata caaaaagata attaaaaaat
     7021 tagaagagga agaaagagtt aaaactctct cacttcaaca aacctattct attgataatc
     7081 aattcgcaga aggattatct ccaataaaac aagaatttca taaaaaacga aatgcttcaa
     7141 gatattacat aaacgagctg gaaagaacaa ctactcaagt ataaaaaaat tagcccaact
     7201 ttaaaaaatg tcaacactga taaacaaaac acaagagata acagaagaac taaaaaaaca
     7261 gaataatatc caagatgtag attataaaat ccttgaagag atagacagaa taatgaaaga
     7321 tattcatcaa gactatataa ttaaatctta tcactcttgg attgaatcag aaaattgtta
     7381 tgtttaccaa aacttaaatt aaaatgtcaa gattaatcaa cccagtagaa agaatgttaa
     7441 aaaaagcaat taaaaacggg gaagtaaaat tattagatga aacagaatat aatgatatat
     7501 gggataaagt tgaacaagat atggaagatt acaagataat ggataattac agaaaaggaa
     7561 tttcgctgat tgaacccaat gaaattttaa tttatagaaa tttcaacaaa ttctacaaaa
     7621 actttcaccc ctctagaaca gcaaaagata tataaacaag tgcctccttg
//
LOCUS       DAOPCH010000042         7350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_9122_length_7350_cov_4.161480, whole genome shotgun sequence.
ACCESSION   DAOPCH010000042 DAOPCH010000000
VERSION     DAOPCH010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9122_length_7350_cov_4.161480"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="PLU55_03760"
     CDS             <1..1291
                     /locus_tag="PLU55_03760"
                     /inference="COORDINATES: protein motif:HMM:NF020810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPJ87203.1"
                     /translation="MKTKRVKKILKEITEKIRPPEEDLKKIKKGVDSFCKEIKSNIKK
                     YKIDAEIFVGGSFAKKTVIKKDRYDVDVFIRYDKKYKNTEISDITRKLIDKSRTISVV
                     HGSRDYFRVDCSEDFFIELIPVRKIKNPKEHENITDLSYLHVKYINRRVKKKNILDEI
                     KIAKAFCYANRCYGAESYIHGFSGYSLELLVYYYKGFEKFINAMAKLNIKKEKLIIDI
                     EKQFKNKKDVLMDLNSSKLNSPIILIDPTYKQRNVLAALSKETFEMFQKSCKEFLENP
                     SISKFELKTIDFNKVRQYSIKRGKDFIRIKTSTSKPEGDVAGSKLLKFYEYLTKEISR
                     FFIVKKRGFEYSNKDYADSFFIAEEKKEIIYPGPYLKDKKNCDNFRKEHKEVYEKAGR
                     LYAKEKINFNLKGFLDKWKTKNTKIINEMYIKEFNYI"
     gene            complement(1282..1584)
                     /locus_tag="PLU55_03765"
     CDS             complement(1282..1584)
                     /locus_tag="PLU55_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87204.1"
                     /translation="MAAWNNLDIGRIIVYRNNSGENKKIAICNYDKEKNHILEILEYP
                     ISLTSKLDVMGLNKNQEVKSEDYLERPGDWCASGNVSSEERSVLEKLAGFSKNLQI"
     gene            complement(1597..1920)
                     /locus_tag="PLU55_03770"
     CDS             complement(1597..1920)
                     /locus_tag="PLU55_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87205.1"
                     /translation="MQEKLVYTAKERIPGQNNFVRINESVGVVLKRLGWKVLNSTYLL
                     SEGLPVEAYGYYVLPGIGVIKTVLTSEDSEYNFHVCILGFEEEKQEYHRLKMYLEDTL
                     RKVLH"
     gene            complement(1922..2233)
                     /locus_tag="PLU55_03775"
     CDS             complement(1922..2233)
                     /locus_tag="PLU55_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87206.1"
                     /translation="MEEQQNYNKKPKYSDNLKRYPLGEGNWLMGIQTSKRFHCWGEIA
                     GKHIDLKGENLQEVEKELYNVLEKLKIDYLNKASKSEEIALRIKENGLEKTSKYLGGK
                     K"
     gene            complement(2235..3029)
                     /locus_tag="PLU55_03780"
     CDS             complement(2235..3029)
                     /locus_tag="PLU55_03780"
                     /inference="COORDINATES: protein motif:HMM:NF022932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPJ87207.1"
                     /translation="MLVKQELINKIKDYFDLNVYETKVWLALIGKGSASAGEIAELSG
                     VPRSRTYDVLETLEKKGFAIVKLGKPVKYLGVKPRMILDKLRNNVRSEAEERIQSLSN
                     IKDTEEFAQLEEIYKGGVDPVKREDVSAAIKGRSNISNYLREIIRNAKREVLICASAR
                     DILSKTKLFQQTFNVLKKENIKIRLCLSGEDNLIKKAENEFGIKIKKVDVIAKFFIID
                     REEILFYLSKPDSKEESAIWLNSEFFSAYFASLFELAFGTDKKEKK"
     gene            complement(3031..3558)
                     /locus_tag="PLU55_03785"
     CDS             complement(3031..3558)
                     /locus_tag="PLU55_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87208.1"
                     /translation="MEKNNSNYKTRRSHIENIASNYTFKVSLSDKPANYVSIKNIPAS
                     MKEAVSCSNFWGDVLNEFHKDINKIDFFYESKSEEVKYNQVSPQAILMVDKPKSLIKM
                     ITNEKEWKRLNEVSKETDDFSAPYVFSLKNLCYRVNIPLECINGVTFNQTDFLNEDYK
                     SPKENSSETGIGGLI"
     gene            complement(3560..3952)
                     /locus_tag="PLU55_03790"
     CDS             complement(3560..3952)
                     /locus_tag="PLU55_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87209.1"
                     /translation="MNTQKENKNLEEKVDDIIKTTSLRNKLKSIENQRKEVKLEDINN
                     ILQRYNTIVDYFVENRDRVESNRTYLVEVMYGLSKCIVPVVYGDSQLKEKDSNFILIK
                     KLSNEIKDMLNIKSEYKSGTGTGVGGLI"
     gene            complement(3954..4235)
                     /locus_tag="PLU55_03795"
     CDS             complement(3954..4235)
                     /locus_tag="PLU55_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87210.1"
                     /translation="MKNSGYLPYHKIRKESINSREYHGNLVDIVCLGRWRLEDEADMK
                     IEAGLAYSPSAIKEKARVMDNLAVLKGYDAYLENILPQFKKSLMGALLN"
     gene            4337..4669
                     /locus_tag="PLU55_03800"
     CDS             4337..4669
                     /locus_tag="PLU55_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87211.1"
                     /translation="MSQNKFGIDKRKSKLEESINKWFFANITGNITIAGYIKSVEGRE
                     ITLNPYKTMRYNSQYGCNLYELVHEDAFLEISPNGYFLDPTNEETIEYIIKKQNEEIL
                     KETIKRED"
     gene            complement(4666..5568)
                     /locus_tag="PLU55_03805"
     CDS             complement(4666..5568)
                     /locus_tag="PLU55_03805"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF018279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presenilin family intramembrane aspartyl
                     protease"
                     /protein_id="HPJ87212.1"
                     /translation="MKHNLKVTLIILAMFVVTQFIGLYVVNHYSVNGHELPYGMEVPE
                     AEQETDFYTSFLPSIVIAFIFAILLLILLTKFNFNFILKLWFFAVILIAIGIFLNTFF
                     PNEKYFPYIALVFALPLAILKIFKRDFLVHNATELVIYPAIAAVFVPLLNIWTVIILL
                     VLISIYDMWAVWRSGVMQKMANYQINELKIFSGFFIPYVSKKVRMEIKKTPKSELKKK
                     KIKVNMAILGGGDVVFPIITSGVVLANFGAVPAIFVILGATLGLSYLLFFAEKKKFYP
                     AMPFITAGIFLGILISWALKLITF"
     gene            5619..6191
                     /locus_tag="PLU55_03810"
     CDS             5619..6191
                     /locus_tag="PLU55_03810"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPJ87213.1"
                     /translation="MAKEIARYHKKNKKTFPVLIGEKITLRPIVEDEFYEKYYQWLRQ
                     IEIIDGIGEEEDMSIQEIIQMHNEWREDISNLTLGIYDNETGKPVGDINLVDSPDFKK
                     GPEIGIMIGDRGKGFGKEALKLMIDYAFNSIHVSQINLDVYKNNPASNLYQKMGFKIT
                     EELKEPETGRDEYKMALTKKEWKEMNKDKK"
     gene            complement(6194..6415)
                     /locus_tag="PLU55_03815"
     CDS             complement(6194..6415)
                     /locus_tag="PLU55_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87214.1"
                     /translation="MASRIREYFERHYSFLKGLASIGVSGIYQPKLPSWMKEGLTPQE
                     QDYLATKGDWEKVGSDLEKAMKNTELNKE"
     gene            complement(6488..>7350)
                     /locus_tag="PLU55_03820"
     CDS             complement(6488..>7350)
                     /locus_tag="PLU55_03820"
                     /inference="COORDINATES: protein motif:HMM:NF022449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HPJ87215.1"
                     /translation="YTGDMKYGKTRLLDPAVNKYTRVESIQVESTYGSKEDILPPRKD
                     TEKKFIDMVRETIEKGGKVLLPELGLGHAQETLLRVEEAIRTGELPKIPMYIDGMIWD
                     INAIHTAYPDFLSANIRSSIFQDKNPFVSDIFVRVASPQERKQVIEGGPCIVIATSGM
                     LVGGASVEYFRYFADNPNNLIILSSYQGPGSLGRKLQDGEKVVSIEGERGMQEIKVNM
                     RVELLSGLTPHSGRNELMSFFNNMYPKPKRILINHGEVSKSLDLASSLYKVHRVETTV
                     PRNLEIVRLR"
BASE COUNT         2603 a         1111 c         1003 g         2633 t
ORIGIN      
        1 aatgaaaaca aaaagagtaa aaaaaattct aaaagagata actgaaaaaa taagacctcc
       61 tgaagaagat ttaaagaaaa taaaaaaagg tgttgattct ttctgtaaag aaataaaatc
      121 aaacataaag aaatacaaaa tagatgcaga gatttttgtt ggagggagtt ttgcaaaaaa
      181 aactgttata aaaaaagaca gatatgatgt agatgttttt ataagatatg ataaaaaata
      241 caaaaacaca gaaatttcag atataaccag aaaattaata gataaatcaa gaacaataag
      301 tgttgttcat ggttccagag attattttag agtagactgt tctgaagatt tttttataga
      361 attaattcca gttagaaaaa taaaaaatcc taaagaacac gagaatataa ctgatttatc
      421 ttatcttcat gttaaataca ttaacaggag agttaaaaag aaaaatatac tagatgagat
      481 aaaaattgca aaggcttttt gttacgcaaa tagatgttat ggggcagaat catatataca
      541 tggtttttct ggatattcct tggaattact cgtttattat tacaagggat ttgaaaaatt
      601 cataaatgct atggctaagc taaacatcaa aaaagagaaa ttgataattg atatagaaaa
      661 gcagtttaag aataaaaaag atgttttaat ggatttaaac agctctaagc tgaattctcc
      721 tattatcctc atagacccaa catacaaaca aagaaatgtt ttagctgcat taagtaaaga
      781 aacctttgaa atgtttcaga aatcctgcaa agaattcctt gaaaatccct ctataagcaa
      841 gtttgagttg aaaacaatag atttcaataa agtgaggcaa tattcaatta aaagaggaaa
      901 ggactttatt agaattaaaa ctagcacaag caaacctgaa ggagatgtag caggtagcaa
      961 actcctcaaa ttttatgaat atttaacaaa agaaataagt agatttttta ttgtgaaaaa
     1021 aagaggattt gaatattcca ataaagatta tgcagattca ttttttatag cagaggaaaa
     1081 gaaagagata atttatcctg ggccttattt aaaagataaa aagaactgtg acaatttcag
     1141 aaaagaacat aaagaagtct atgaaaaagc aggaagactc tatgctaaag agaagattaa
     1201 ttttaattta aaaggatttt tagataaatg gaaaactaaa aatacaaaga taataaacga
     1261 aatgtacata aaagagttta attatatttg aagattctta gaaaaaccag ctaatttctc
     1321 taatacgctt ctttcttctg aagaaacatt tcctgatgca caccaatctc cgggtctttc
     1381 aagataatct tcacttttaa cttcctgatt tttatttaaa cccattacat ctaatttaga
     1441 agttaaagaa attgggtact ccaaaatctc taatatatgg tttttctctt tatcataatt
     1501 acatattgct attttcttat tttcgccact attatttcta taaacaatta tccttcctat
     1561 gtccaaatta ttccaagctg ccattctgta aattaatcag tgtaaaactt ttctcaaagt
     1621 atcttcaaga tacatcttaa gtctgtgata ctcttgcttt tcttcttcaa atcctaaaat
     1681 acaaacatga aagttgtatt ctgaatcttc tgaagttaaa actgttttta taactcctat
     1741 acctggaaga acataatatc catatgcttc aacaggtagg ccttctgaaa gcaaataagt
     1801 gctgtttagg actttccatc caagtctttt caatacaaca cctacagact cgtttatcct
     1861 tacaaagttg ttttgccctg gaattctttc tttcgcagtg taaactagtt tttcttgcat
     1921 tttatttttt tcctcctaaa tatttagaag tcttttcaag accattttct tttattctaa
     1981 gagcaatttc ttctgatttg cttgccttat tcaaataatc tatttttagt ttttcaagaa
     2041 cattgtataa ttctttttct acttcttgaa gattctcacc tttaagatca atgtgttttc
     2101 cagcaatttc tccccaacaa tgaaatcttt ttgaagtttg aattcccata agccaatttc
     2161 cttctcccaa agggtatctt tttaggttat ctgaatattt tggtttttta ttgtaatttt
     2221 gttgttcttc cattttactt tttctccttt ttatctgtgc caaaagctaa ttcaaacaat
     2281 gaagcaaaat atgctgaaaa gaattctgaa ttaagccaga ttgctgattc ttctttgctg
     2341 tctggtttag ataaataaaa taaaatttct tctctgtcta taataaagaa tttagcaata
     2401 acatcaactt tctttatttt tattccaaat tcattttctg ctttctttat taaattatct
     2461 tctcctgaaa ggcagagtct tatcttgatg ttttcttttt tcaaaacatt aaaagtttgt
     2521 tggaataatt ttgtttttga aagtatatct cttgcacttg cacatattaa tacttctctt
     2581 ttagcatttc ttattatttc tcttaaataa tttgaaatat tagaccttcc ttttattgca
     2641 gcagaaacat cttctctttt aacagggtct actccacctt tgtaaatttc ttcaagttga
     2701 gcaaattctt ctgtgtcttt tatatttgaa agactttgta ttctttcttc tgcttcactt
     2761 cttacattat ttctaagttt gtcaagaatc attcttggtt ttacacccaa atatttaaca
     2821 ggtttaccaa gtttaactat tgcaaaacct tttttttcaa gtgtttctaa aacatcataa
     2881 gttctacttc ttggaactcc tgaaagctct gcaatttcac ctgcagaagc tgagcctttt
     2941 ccaattaaag caagccatac ttttgtttca taaacattca aatcaaaata atctttgatt
     3001 ttattaatta attcctgttt aactaacatc ttaaattaaa ccccctatac ctgtttctga
     3061 actgttttct ttaggacttt tataatcctc attcaaaaaa tcagtttggt tgaaagtaac
     3121 tccatttata cattctaaag gaatatttac acggtaacat agattcttaa gtgagaaaac
     3181 atatggagca ctaaaatcgt ctgtctcttt tgaaacttca tttaatcttt tccattcttt
     3241 ttcatttgta atcatcttaa ttaaactctt aggtttatca accataagaa ttgcttgtgg
     3301 gctaacttga ttatacttca cttcttctga cttagattca taaaaaaagt ctattttatt
     3361 aatatcttta tgaaattcat ttaaaacatc tccccaaaaa ttagaacaag aaactgcttc
     3421 tttcatagat gctggaatat tttttataga aacataattt gcaggtttat ctgaaagaga
     3481 tactttgaaa gtgtaatttg aagctatatt ctcaatatgg cttcttcttg ttttataatt
     3541 agaattgttt ttttccattt taaattaaac ctcctacacc tgttcctgtt ccacttttat
     3601 attctgattt aatattaagc atatctttta tttcattaga tagtttcttg attaagataa
     3661 aattagaatc tttttccttt aattgactat caccatacac cacaggaaca atacattttg
     3721 ataatccata cattacttct acaaggtatg ttctgttgct ttctactctg tctctatttt
     3781 ctacaaaata atcaactatt gtgttatatc tttgtaaaat attatttata tcttctaatt
     3841 ttacttcttt tctttgattt tctattgatt ttaatttatt tctaagagat gtggttttga
     3901 taatatcatc aaccttctct tccaaattct tgttttcttt ttgtgtgttc attttaatta
     3961 agtaatgctc ccataagact tttcttaaat tgaggtagaa tattttccaa ataagcatcg
     4021 tatcctttta gaacagctaa attatccatt actcttgctt tttcttttat tgcagaaggg
     4081 ctgtatgcta gacctgcttc aattttcata tctgcctcat cttctaatct ccatcttcca
     4141 agacagacaa tatccactaa attgccatga tattctctag aattgatact ttccttcctt
     4201 attttgtgat agggtaaata acctgaattt ttcataaagt tactattctt taggggttta
     4261 taaatatttc tacatgttgt atttcataag tagttacaat ataaagattt ataaatcgtt
     4321 tactggtagg atatttatga gccaaaataa atttggaata gataaaagga aaagcaaact
     4381 tgaagaatct attaataaat ggttttttgc aaatattact ggaaatatca caatagcagg
     4441 atatataaaa agtgtagaag gaagagaaat aacacttaat ccatataaaa ctatgagata
     4501 taattctcaa tatgggtgca atttatatga attagtgcat gaagatgctt ttctggaaat
     4561 atctccaaat ggctattttc ttgatccaac aaatgaagaa acaatagaat atataataaa
     4621 aaagcagaat gaagaaatct taaaagaaac tataaaaagg gaagattaaa aggtgattaa
     4681 ctttaatgcc caagaaatta aaatacctag gaaaatacct gcagttatga aaggcattgc
     4741 aggatagaat ttctttttct ctgcaaagaa caaaagataa ctaagtccta gtgttgctcc
     4801 aaggattaca aatattgcag gaactgctcc aaagttagca agaactactc ctgaagttat
     4861 tattgggaaa acaacatctc ctcctcctaa aatagccata tttactttaa tcttcttttt
     4921 ctttaattct gatttaggag ttttctttat ctccattcta actttcttgg aaacatacgg
     4981 gataaagaaa ccagagaaaa tcttaagctc atttatctga taattagcca tcttttgcat
     5041 aactcctgaa cgccatactg cccacatatc atatatagaa attaatacta gtaagattat
     5101 tactgtccat atatttaaaa gaggaacaaa gactgcagct atagcagggt aaataacaag
     5161 ttctgttgca ttgtgaacta aaaaatctct tttaaatatt tttaatattg ctaatgggag
     5221 tgcaaatact aatgcaatat atggaaagta tttttcattt ggaaaaaagg tgtttaggaa
     5281 aattcctatt gctataagaa taactgcaaa aaaccacagt tttaaaataa aattaaaatt
     5341 aaactttgtg agaagtatga gtaagagtat tgcaaatata aaagcaatta ctattgaagg
     5401 caaaaaagag gtgtaaaaat ctgtttcctg ttctgcttca ggaacttcca ttccataggg
     5461 aagttcatgt ccattaactg agtaatgatt cacaacatat aaaccaataa attgggttac
     5521 aacaaacata gcaagaatta tgagtgttac tttgaggttg tgtttcatgt attatacaag
     5581 atattagggt ttttaaagtt taaataacaa gaatataggt ggcaaaagag atagcaagat
     5641 atcacaaaaa aaacaaaaaa acttttcctg ttcttattgg ggaaaaaata acattaagac
     5701 caattgtaga ggatgaattc tatgaaaaat attatcagtg gttaagacaa atagaaatta
     5761 tagatggtat tggagaggaa gaagacatga gtattcaaga gattattcag atgcataatg
     5821 agtggagaga agacatctca aatttaactc ttggaatata tgataatgaa acaggaaaac
     5881 cagtaggaga tattaattta gttgatagtc cagattttaa gaaaggacct gaaataggaa
     5941 taatgattgg agatagagga aaaggatttg ggaaagaagc acttaaatta atgatagatt
     6001 atgcttttaa ttcaatacat gtatctcaaa taaatttaga tgtgtataaa aacaatcctg
     6061 cttcaaacct ttaccaaaaa atgggattta agataactga agaattaaaa gaacctgaaa
     6121 caggaagaga tgaatacaaa atggcattaa caaaaaaaga gtggaaagaa atgaataagg
     6181 ataaaaaata atattactct ttatttaatt ctgtgttttt cattgctttt tctaaatcac
     6241 ttcctacttt ttcccaatct ccttttgttg caagataatc ttgctcttga ggagttaatc
     6301 cctctttcat ccaagagggt aatttaggtt gatagattcc agaaacacct atactagcta
     6361 aaccttttaa gaaactataa tgtctttcaa aatattctct tattcttgaa gccatattca
     6421 tattagacat atttaattta taaaaatttg tatagttatt atttgagata tgattagaaa
     6481 tataaaatca tctcaatcta acaatttcaa gatttcttgg aacagttgtt tctaccctat
     6541 gaactttgta taatgaagaa gctaaatcca aagactttga aacttctcca tgattgataa
     6601 gtattctttt tggttttgga tacatgttat tgaagaaaga cattaattca tttcttcctg
     6661 agtgaggagt taaaccactt aataactcta ctctcatatt tacctttatt tcctgcattc
     6721 ctctctctcc ttcaatagaa actacttttt caccatcttg taattttctt ccaagtgaac
     6781 ctggaccttg atatgaactc aatataataa ggttatttgg attgtctgca aagtatctga
     6841 aatattcaac agaagctcct ccaacaagca tacctgaagt tgcaataact atgcaaggac
     6901 ctccttcaat aacttgtttt ctttcttgag gtgaagcaac tcttacaaaa atatctgaaa
     6961 caaatggatt tttatcttgg aaaattgaag aacgtatgtt tgcacttaaa aaatcagggt
     7021 atgctgtgtg aatagcatta atatcccaaa tcataccatc aatatacata ggtatttttg
     7081 gaagttctcc tgttcgtata gcttcttcaa ctcttaaaag agtttcctga gcatgaccta
     7141 aacctaattc tggaagaaga acttttcctc ctttttcaat tgtttctctt accatatcaa
     7201 taaatttctt ttcagtatct tttcttggag gaagaatatc ttctttgctc ccataagtag
     7261 attctacttg aattgattct actcttgtat atttgtttac agcagggtct aaaagtcttg
     7321 tcttgccgta tttcatatct cctgtatata
//
LOCUS       DAOPCH010000043         7125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_9449_length_7125_cov_5.166902, whole genome shotgun sequence.
ACCESSION   DAOPCH010000043 DAOPCH010000000
VERSION     DAOPCH010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 7125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7125
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9449_length_7125_cov_5.166902"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PLU55_03825"
     CDS             <1..641
                     /locus_tag="PLU55_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HPJ87216.1"
                     /translation="DVFGQVRHIVGLIEKGIFDSVHWQIDAGFYKSDFNKKQFKLFVK
                     EYNRSIDRLLDFWVDYMKENKKVLKFYPFLGIFECLHYNRKTKLHCGSGYANYTILTN
                     GKIVACPIMSGIKNFYCGDLASPNETLKEIYVQEPCSSCEYLDVCGGRCLYSNYAQLW
                     PEEGQKLICESVIHLIQGVYDVLPKIEDLIEKKIILEYDFDYEKYFGPEIIP"
     gene            complement(657..1508)
                     /locus_tag="PLU55_03830"
     CDS             complement(657..1508)
                     /locus_tag="PLU55_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPJ87217.1"
                     /translation="MIYFPIIAAIALAGGTVLERHILKRKEIDIRRYQTLGFLTISLI
                     LLPMLYFFWKLDPQALQLKNILILIAVVICSMVANLFTFYSMKGEKISNLEPAKMLEP
                     LFTILLAVLFSLFFQGAYESNPKVLVASLVAGLVLILTHINKEQLRFNKYFTAAIIGS
                     FFFALEIIISNLILELYSPVTFYFLRCFGIFVFSLILFRTKLSGVDKKLKIDLVTVGI
                     IWVLYRVVAYYGYLKIGIVSTTLILMLGAALIYLFAKIFLKEKINKKNIIASIVILAC
                     IIYASFA"
     gene            1791..2135
                     /locus_tag="PLU55_03835"
     CDS             1791..2135
                     /locus_tag="PLU55_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87218.1"
                     /translation="MKKALDKVGGWAFIIGLVLAILIAIFGAEATWPIYLLLFLGIVV
                     GILNIGKKEVSSFLLAAVAFMFTFTSLGKVVEIIPVLGEPLARFFSLMNAFVAPAAAV
                     VAFKALFAHSKH"
     gene            2228..2461
                     /locus_tag="PLU55_03840"
     CDS             2228..2461
                     /locus_tag="PLU55_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87219.1"
                     /translation="MRKECALKIIFIISIAGLLFSGYLSYTELIVGYCAVSDSGAGGC
                     TNIAGIPVCVYGFVMYLVIFVISLLGLRSKSKR"
     gene            2505..3008
                     /locus_tag="PLU55_03845"
     CDS             2505..3008
                     /locus_tag="PLU55_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87220.1"
                     /translation="MNTRFIFLGIFLTIFLIIGVVYILKKRKGSIELIPEKYNYNLGE
                     KLSGKLVLKLRKPANNSKLIIGLKCQRSETTYSNNEKSTSHYTLFDFNFPLENNKDYT
                     PGEYSYDFSIDIPKNYSKDVGPIAGNIIKSINFLRGRNSFLNWYLYSELNCSGVDLNK
                     EIKINVS"
     gene            complement(2995..5424)
                     /gene="ppsA"
                     /locus_tag="PLU55_03850"
     CDS             complement(2995..5424)
                     /gene="ppsA"
                     /locus_tag="PLU55_03850"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HPJ87221.1"
                     /translation="MDKKKERVIWFKDLTIEDVPLVGGKNASLGEMYRELTKKGVRIP
                     NGFAITANAYRYFLESAGIKDKIKKILSDLDTHNIRNLSERGEKVRDTILSGELPKDL
                     EEEIIKNYELLCKYYKTNNVDVAVRSSATAEDLPDASFAGQQETFLNIKGRRALIESC
                     KKCFASLFTNRAISYRVDKGFDHFNIALSIGVQKMVRSDLACSGVMFSIDTESGFKDA
                     VFITSSYGLGENIVLGAVNPDEYYVHKPTLKKGFKPIISRKIGDKLIKLVYSNEGTKP
                     TKNVSVDPDDRKKFSLTDEEVLQLAKWACIIEDYYSEKAKKFRPMDIEWAKDGQTKKL
                     FIVQARPETVISQKDHNVLEEYILKQKGKLIVEGGSVGSKIGVGKANVIKDVSGIYNF
                     KKGEVLVTDMTDPDWEPIMKIASAIVTNRGGRTCHAAIISRELGIPCVVGTNKGTEKI
                     KKGQHVTVSCSEGERGRVYEGILKFVVQKHNLKEIGKTKTKIMMNIGSPDQAFEQSFT
                     PNDGVGLAREEFIINSNIKIHPMALVNYKKLPEGKVKEEIDELTQGYKNKEQFFIDKL
                     AEGIGMIAAAFYPKEVIVRTSDFKTNEYANLIGGKEYEPKEENPMIGWRGASRYYKKE
                     YKEGFVLECKAFKKVREEFGLKNVKLMIPFCRTVEEGKKVLKIMEENGLKRGKDGLEV
                     YVMCEIPSNVILCDEFSKIFDGFSIGSNDLTQLVLGLDRDSSIVSDIYDERNEAVKRF
                     IAEVIKKCKKYNRKIGICGQAPSDFPDFAQFLVECGIDSISLNPDTVIKTKIAIAETE
                     KKLRKQNFKKH"
     gene            complement(5495..5565)
                     /locus_tag="PLU55_03855"
     tRNA            complement(5495..5565)
                     /locus_tag="PLU55_03855"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5531..5533),aa:Gly,seq:gcc)
     gene            complement(5623..6594)
                     /locus_tag="PLU55_03860"
     CDS             complement(5623..6594)
                     /locus_tag="PLU55_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPJ87222.1"
                     /translation="MELNLKDRKILYELDKNSRIGLTELAKKIQMSKESLYYRLKKLE
                     DEKIILKYHTVSAHYRFGTVDYKVYLRLKDLSKKKYESLIDFLMENKDVFWIAPCNGR
                     WDLMFGIRAIQMNEFFEIHDMLLEKFSKYIQEKELSISRNALQFNRRWMLDSKEERKT
                     FEFGEENKKIELDKEDRLILNELVNNSRKKIVDISKNTNLSVDVVNYRIKKMEKQKII
                     NGYKCLFNVEKLGLITTKAFVYFKNINQERKKEFIQHIKSLKNSVNVVTTFAPWDLEI
                     IFEIEDYRDYYKIMEEVKDKFSDIIKFYDSVIIFEERKQVFDRTEKI"
     gene            6689..7072
                     /locus_tag="PLU55_03865"
     CDS             6689..7072
                     /locus_tag="PLU55_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87223.1"
                     /translation="MKRQNILYHGTCRAFLAYALENKCLFGDENGKLSLTTELSHAID
                     YAEQWSTKGGFKSIEKYFGEDIEKSLCEPILLELNSKTIKKIKNLRYNNDCGRGEYYL
                     EGNLSLDFVKQIYFKEGIEDLKGGR"
BASE COUNT         2281 a         1039 c          965 g         2840 t
ORIGIN      
        1 cagatgtttt tggacaagtt agacacatag ttggattaat tgagaaaggt atatttgatt
       61 ctgttcattg gcagattgat gctggatttt acaaatctga ttttaataaa aaacagttta
      121 aactttttgt aaaagaatat aataggtcta tagatagatt gttggatttt tgggttgatt
      181 atatgaaaga aaataagaaa gttttgaaat tttatccttt tttaggaata tttgagtgtt
      241 tacattataa tagaaaaaca aaacttcatt gtggttctgg atatgcaaat tacactattt
      301 taactaatgg taaaattgtt gcttgtccta ttatgagtgg tataaagaat ttttattgtg
      361 gggatttggc ttctcctaat gaaacactta aagaaattta tgttcaagaa ccttgtagtt
      421 cttgtgaata tctggatgtt tgtggaggaa gatgtttgta tagtaattat gctcaacttt
      481 ggcctgaaga aggacaaaag ttaatctgtg aaagtgtaat ccatttaatt caaggggttt
      541 atgatgtatt acccaagata gaagatttaa ttgaaaagaa aattattttg gaatatgatt
      601 ttgattatga gaaatatttt ggtcctgaga ttataccgta aattttttat tagctattaa
      661 gcaaaactcg cataaataat acaagccaga ataactattg aagcaattat atttttttta
      721 tttatttttt ctttaaggaa aatttttgca aataagtaga ttagagctgc tcctaacata
      781 agtattaatg ttgttgaaac aattcctatt tttaggtatc cataatatgc aacaactcta
      841 tataaaaccc agattattcc aactgtaact aaatctattt ttaatttctt atctactcct
      901 gaaagttttg ttctgaagag aattaggcta aatacaaata ttccaaaaca tctcagaaaa
      961 tagaaagtta caggggaata aagttcaagt attaaattag atattattat ttcaagagca
     1021 aaaaagaaac ttcctattat tgctgcagtg aaatatttgt tgaatcttaa ttgttcttta
     1081 tttatgtgtg ttaatataag cactaaccct gctacaagag atgcaactaa aacttttgga
     1141 ttgctttcat atgctccttg aaaaaacaaa ctaaataata ctgcaagcag aatagtaaac
     1201 aatggttcaa gcatttttgc tggttctaaa ttgcttattt tttcaccttt cattgaataa
     1261 aatgtaaaaa ggtttgcaac catcgaacag attacaactg caattaagat tagtatgttt
     1321 tttaattgta aagcttgagg gtctaatttc cagaaaaaat aaagcatagg aagcaatatt
     1381 aaagagattg ttaaaaatcc taatgtttgg tatcttctga tatcaatttc ttttcttttc
     1441 agaatatgtc tttcaaggac agttcctccc gccagtgcga ttgcagctat tattggaaag
     1501 tatatcatct cttttagtta atgctaagtt attagctatt aaaaaacttt tgaaaaattt
     1561 ataatgaaaa agcttaaaag tgctgaaaag ttttactttt taagggccta taagctagcg
     1621 gtaaactgac tcgtttacat gtttggtcga ttaagaatta acaaactaaa agccgagtat
     1681 tcagcggttc gactccgctt aggcccattt gttatttaat tcttatttgc attttcaaat
     1741 catttatata ctctaattac tctgtattct taattaaatt ggaggtgtaa atgaaaaaag
     1801 cacttgataa agtaggtgga tgggcattta taattggctt agttttagct attttaattg
     1861 ccatatttgg tgctgaagca acttggccaa tatacttatt gcttttcctt ggaattgtag
     1921 taggtatttt gaatataggc aagaaagaag ttagctcgtt tttactagca gcagttgcat
     1981 ttatgtttac ctttacttct ctaggtaaag tagtagagat aatacctgtt ctaggagaac
     2041 cattagctag attttttagc ttaatgaatg catttgttgc tcctgcagct gcagttgttg
     2101 cattcaaagc attgtttgct cacagtaagc actaaaaata ttttttgttt ttattttttg
     2161 tttttctaaa tctaaatgat atattattta tcaaaaatat tataaataat aatttattat
     2221 aattcttatg agaaaagagt gtgcattaaa aattatattt ataatttcaa tagcaggatt
     2281 gttgttttca ggttatttga gttatactga attaattgtt ggatattgtg cagttagtga
     2341 ttcaggagct ggaggatgta caaatattgc tggaatccct gtttgtgttt atggttttgt
     2401 tatgtatctt gttatatttg ttatttcttt acttggatta agaagcaaga gtaaaagatg
     2461 aattattaag atactttttt aaattagtgt ttatttaata atatatgaat acccgattta
     2521 tctttttagg tattttctta acaatatttc ttattattgg tgttgtttat attttgaaga
     2581 aaagaaaagg ttcaattgaa ttaattccag aaaaatacaa ttataatttg ggagagaagt
     2641 taagtggtaa gcttgttttg aaacttagga aaccagcaaa taacagtaag ttaattatag
     2701 gtttaaaatg tcaaaggtca gaaacaacat attcaaataa tgaaaaaagc actagtcatt
     2761 atactttgtt tgactttaac tttccattag aaaataataa agattatact cctggagaat
     2821 attcttatga tttttcaata gatattccta aaaattattc taaagatgtt ggacctatag
     2881 ctggaaatat aataaaatca attaattttc ttagaggtag aaattctttt ttgaattggt
     2941 atttgtattc tgaattaaat tgttcaggtg ttgatttaaa taaagagatt aagattaatg
     3001 tttcttgaaa ttttgttttc taagtttctt ttctgtttct gcaattgcta tctttgtttt
     3061 tattacagtg tctggattca atgaaataga atcaattcca cattcaacta agaattgtgc
     3121 aaaatcaggg aaatctgaag gagcttgtcc acatattcct atttttctgt tatatttttt
     3181 gcattttttt attacttctg caataaatct ttttacagct tcatttcttt catcataaat
     3241 atctgaaact attgaagaat ctctgtctaa tcctaaaact aattgagtta aatcattact
     3301 tccaattgag aacccatcaa atatttttga gaattcatca cacaatatta catttgaagg
     3361 aatttcacac ataacatata cttctaatcc gtcctttcct cttttaagtc cgttttcttc
     3421 cattatcttt aatacttttt ttccttcctc aactgttcta caaaaaggaa tcattaactt
     3481 tacatttttt aatccaaatt cttctcttac ttttttgaaa gctttacatt caaggacaaa
     3541 tccttctttg tattcttttt tataatatct tgaagctcct ctccatccaa tcattggatt
     3601 ttcttctttt ggctcatatt cttttcctcc aattagattt gcatattcat ttgttttgaa
     3661 gtcacttgtt cttacaataa cttcttttgg ataaaatgca gctgcaatca ttcctatacc
     3721 ttcagcaagt ttatcaataa agaattgttc tttgtttttg tatccttgag ttaattcatc
     3781 tatttcttct tttacttttc cttcaggtaa ttttttgtaa tttactagag ccatagggtg
     3841 tatctttatg tttgagttta taataaattc ttctcttgca agtccaactc catcattagg
     3901 tgtaaagctt tgttcaaatg cttgatctgg agatccaata ttcatcatta tctttgtttt
     3961 tgttttacca atttctttta agttgtgttt ttgaacaaca aacttgagaa tcccttcata
     4021 tacacgtcct ctttctcctt cagaacaact tacagttaca tgttgtcctt tttttatttt
     4081 ttcagttcct ttatttgtac ctacaacaca cgggattcct aattctcttg aaataattgc
     4141 agcatgacaa gttcttcctc ctctatttgt tacaattgct gaagctattt tcattatagg
     4201 ttcccaatca gggtcagtca tatctgtaac taaaacctct ccctttttga aattgtaaat
     4261 tccagaaaca tcttttataa catttgcttt tccaactcca atcttagagc ccacacttcc
     4321 tccctcaaca attagctttc ctttttgttt taaaatatat tcttctaaaa cattatggtc
     4381 tttttgactt ataactgttt caggtcttgc ctgaacaatg aataattttt ttgtttgtcc
     4441 atcttttgcc cattcaatat ccattggtct gaattttttt gctttttcag aataataatc
     4501 ttcaattatg caagcccatt ttgctaactg caacacttct tcatctgtca aagagaattt
     4561 ttttctatca tcagggtcaa ctgaaacatt ctttgttggc tttgttccct catttgaata
     4621 gactaattta attaatttat ccccgatttt tcttgagatg attggtttga atcctttttt
     4681 cagtgttggt ttatgaacat aatattcatc tggatttact gctcctaaaa caatattctc
     4741 tcctaatcca taagaagaag ttataaaaac agcatcttta aatccagatt ctgtatcaat
     4801 agagaacatt actcctgaac atgctaaatc gcttcttacc attttttgta ctccaattga
     4861 aagagctata ttgaaatggt caaatccttt atctactctg taagagattg ctctgtttgt
     4921 gaaaagagaa gcaaaacact ttttacaaga ttctattaat gctctgcgtc cttttatatt
     4981 aaggaaagtt tcttgttggc ctgcaaaact tgcatcaggt aaatcctcgg cagttgcaga
     5041 gcttcttact gcaacatcaa cattatttgt tttatagtat ttacataata attcataatt
     5101 ttttattatc tcttcttcta aatcttttgg aagttcacca gataagatag tgtctcttac
     5161 tttctctcct ctttcagaaa gatttcttat attgtgggtg tctaaatcag aaagaatttt
     5221 ttttatttta tcttttattc ctgctgattc aagaaaatat ctgtaagcat tcgcagttat
     5281 tgcaaaacca tttggaattc taactccttt ttttgttaat tctctataca tttcaccaag
     5341 agaagcattt tttcctccaa ccaaaggaac atcttcaatt gttaaatctt taaaccatat
     5401 aactctttct tttttcttgt ccatttttca ccacttttta ttaatatatt attaatataa
     5461 taaaactagt ttttaaacat ttcttaatgt aaaatgcgct ccccgggaat cggacccggg
     5521 ccacctcgtt ggcaacgaga tattctacca ttaaactagg aacgcatcca gtttatttta
     5581 tacaagaaga ggattattta aagattgttg agagcggaat tattaaattt tttctgttct
     5641 gtcaaagact tgttttcttt cttcaaatat tataacacta tcataaaact ttattatatc
     5701 tgagaattta tcttttactt cttccattat tttataataa tctctataat cctctatttc
     5761 aaatattatt tctaaatccc atggtgcaaa agtcgtcaca acattcacag aattttttag
     5821 agattttata tgctgaatga attctttttt tctttcttga ttaatgtttt taaaataaac
     5881 aaaagccttt gttgttatta gtcccaattt ctcaacatta aaaagacatt tatagccatt
     5941 aattattttt tgcttttcca ttttctttat cctgtaatta acaacgtcta cagaaagatt
     6001 tgtgttcttt gaaatgtcta ctatcttttt tcttgaatta tttactagtt catttaaaat
     6061 taatctatcc tctttatcta gctctatttt tttattttct tctccaaatt caaaagtttt
     6121 tctttcttct ttagaatcta gcatccatct tctattaaat tgtaaagcgt ttctactaat
     6181 agataattcc ttctcttgaa tatatttact aaatttttct aagagcatat catgtatctc
     6241 aaagaattca ttcatctgga ttgccctaat tccaaacatc aaatcccatc ttccattaca
     6301 gggagctatc caaaaaacat ctttattttc cattagaaaa tcaattaagg attcatactt
     6361 tttttttgaa agatctttta gtcttaaata taccttataa tctactgttc caaatctata
     6421 atgtgctgat actgtatggt attttaggat tattttttca tcttctaact tttttaatct
     6481 ataataaagt gactctttgc tcatttgtat tttcttcgca agttctgtta atcctattct
     6541 agaattttta tctaattcat acaaaatctt tctatctttt aaatttagtt ccatatttta
     6601 acatattata atttatttat atatttatcc ttttttgtta aaaatatttg aaataagttt
     6661 taattagtat attatttgta ttactactat gaaaagacaa aatatactat atcatggaac
     6721 ttgcagagca ttcttagctt atgctttaga aaataaatgt ctatttggcg atgaaaatgg
     6781 gaaattaagt cttaccaccg aattgtccca tgcaatagat tatgcagagc agtggagtac
     6841 taaaggagga tttaagagta ttgaaaaata ttttggagaa gatattgaaa aatcgctatg
     6901 tgaaccaata ttattagaat taaattcaaa aacaattaaa aaaattaaaa atttaagata
     6961 taacaatgat tgtggaagag gcgaatatta tttagaaggt aatttaagct tagattttgt
     7021 taaacaaatc tattttaaag aaggaataga agacttgaaa ggaggtagat aaactattga
     7081 aaaaatgatg atatttgaaa aacaaggaaa atatgataaa taaga
//
LOCUS       DAOPCH010000044         6974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_9680_length_6974_cov_11.848967, whole genome shotgun sequence.
ACCESSION   DAOPCH010000044 DAOPCH010000000
VERSION     DAOPCH010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9680_length_6974_cov_11.848967"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..380
                     /locus_tag="PLU55_03870"
     CDS             90..380
                     /locus_tag="PLU55_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87224.1"
                     /translation="MNAKELIEKLRRARPKLALYLENTRKRENEYIIYNLKDIDQKVY
                     HTFKAKGFFYAIQYANEWAKNNHAVITGTTLFDCKDYINRTIEYSCGCCKND"
     gene            373..522
                     /locus_tag="PLU55_03875"
     CDS             373..522
                     /locus_tag="PLU55_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87225.1"
                     /translation="MTKFFSDNETRKKKISETLKNYFKSEKGKEHKEKIKKAKIEYWK
                     KIKGE"
     gene            525..863
                     /locus_tag="PLU55_03880"
     CDS             525..863
                     /locus_tag="PLU55_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87226.1"
                     /translation="MEKQIDIRAYIENAVDFQLKKKEYPEKEIKKKEDIENKIIYVYD
                     YQKIVRENEIKSFYVFDCIDENEVPFSFFGSDILASQISDNILPIKTKLIKVQTKNNL
                     AGYYWSFSRP"
     gene            910..1431
                     /locus_tag="PLU55_03885"
     CDS             910..1431
                     /locus_tag="PLU55_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87227.1"
                     /translation="MGFLDKIKKIFESKEKKKARKIEKITSDLSRKQKLPKKESYKYK
                     ESVKPISAKYHHIVKIYFVRDYGDLDDDIIDLMIKFVKKYKDRFIAKEINKAIDYTNE
                     PKLKDMFETEFETELFIEIITLASERKRLTINELWQYSQEFLDTKAKNDGSAKAILVG
                     LTKMGLVKNELQY"
     gene            1415..3241
                     /locus_tag="PLU55_03890"
     CDS             1415..3241
                     /locus_tag="PLU55_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HPJ87228.1"
                     /translation="MSYNIKVNKNFHFLTKNEKTTMPSHIICFDTETEVIDNQHKLKL
                     GWAFYCKIENDKITRENKFFFTKKEQFWEFIKKYAYKNTKVYIFAHNTQFDFMIVGGS
                     VELQKHNLYIDNDFIDNGNFILSVKEKNKINEKKHYTGTNYIFLDTMNYVKKSLSSIG
                     EMIGLEKMKIDFKTCSFEYLKKYCYRDVEIIQKFILEWIDFLKSNDLGNFKYTLASQS
                     FSTYRHRFMNSDIGIHNIINAIELERKSYKGGRNEVFKFGKDFAYIYDVNSMYPYVMA
                     NNRYPTKIIGFYRNGNKSSIERAFNKGFGIIADVDISTTENIIGIKDERLFFPIGQFR
                     CQITTPEIKYLLNNGHKILKVHNYAIYEMDYIFKDFIEFFYNERLKARNENNLIKSEM
                     YKILMNSLYGKFGQKINSWLDIGEYDIKNDTDRIEQIYNVDTKKYTLLKYFGGKIYKN
                     DGYVEGYNSFVAIPSFVTAYSRVYLFELMKIAGLENVLYCDTDSLFLTVDGHNNIKNS
                     PYVDDKKLGCLKLEKKGNVNIFGCKDYNFNKERKTKGVTKNAKVLDKNKFEYTQFTKL
                     RTALRKNLLDGVYINNIEKTLKQEYKKAIVKDNKTTPFRLDL"
     gene            3264..3704
                     /locus_tag="PLU55_03895"
     CDS             3264..3704
                     /locus_tag="PLU55_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87229.1"
                     /translation="MVIIEKDINKNIETEKDIKKLKRTDKGVCASCKRHRIIKDFDLY
                     LCKTCLNAKKYWIGFNEKKINNNIDNDISVSKEIETEAKIEPISYFCDYCNNKIAYAS
                     KKCNKCGNILNWLGTKLEYDTNYLICGSCGMILEFNANKCERCL"
     gene            3706..4155
                     /locus_tag="PLU55_03900"
     CDS             3706..4155
                     /locus_tag="PLU55_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87230.1"
                     /translation="MVKEIVNIPNEISLIPQSNQPIISNPMGIAQSGISDIENYIKLI
                     EKAIDLIDKLKSLSGIKTETNTIINNTPHPSVSDMPRLNTNSNNEIIDNDIEGEKMNI
                     NIDLILNILKQIEAFKKDMTITELIKIIENDKDTIQKIINANMDMIK"
     gene            4152..4739
                     /locus_tag="PLU55_03905"
     CDS             4152..4739
                     /locus_tag="PLU55_03905"
                     /inference="COORDINATES: protein motif:HMM:NF017518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HPJ87231.1"
                     /translation="MSKRITIVGLQGSGKTQLAKKMIRDKVRKNKKVLVFDILGEYVK
                     DKSLGNIDVFIPQDRIEPLLEIDTVLKNYVVEPYIYDKKKNYSLIVFDEASRYFNNKL
                     KLPSNMGFINDMARHIELDIICIARRFTQLNTDIVELSHYLYIFNQAGINDIKRLNEL
                     SIGLGDTVLTLNKYHYIEVNQFRQYSIKKPIKIMD"
     gene            4913..5098
                     /locus_tag="PLU55_03910"
     CDS             4913..5098
                     /locus_tag="PLU55_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87232.1"
                     /translation="MIGNGTMMSGAIKLGAGLMSRKFLGRGILGDAVSLGFAIDGVED
                     ILFGLHGGLPRNIEGGW"
     gene            5101..5607
                     /locus_tag="PLU55_03915"
     CDS             5101..5607
                     /locus_tag="PLU55_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87233.1"
                     /translation="MVDTQMVGKFRLVHYDYNDTALAVLTEKHAYEFAKGNTAPTDRQ
                     TQPSININDSTIIKEDDKLVVLVNLDANITEHSASSQRNLWTWQIPITFKNERTGNKF
                     KKTLSVNDFSFTGAEAPANSKAWVSGKWYMLGYYQVPAQSSIKLGHTIQDVRVDSKII
                     LHQALTTS"
     gene            5661..6101
                     /locus_tag="PLU55_03920"
     CDS             5661..6101
                     /locus_tag="PLU55_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87234.1"
                     /translation="MTDKWTYGKSKKYDIYTPYRYPINRVPPENRSFLSGFKQVIEIP
                     ALISKSFAQYDKENSEIIKMGVKRYGWFNSFTVLNQSDQIVEISLDYSNNKKYVIPAG
                     VQTSISDVLFESFNIKNIGNGNIASDGLVKVYVIYERPIIREGV"
     gene            6104..6298
                     /locus_tag="PLU55_03925"
     CDS             6104..6298
                     /locus_tag="PLU55_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87235.1"
                     /translation="MDYKKFLSRKFIAMIIGIICFIMTTLMPELDYQSLENIIYIIMA
                     YIFAEAGIDAVAVHRKNGGE"
     gene            6302..6664
                     /locus_tag="PLU55_03930"
     CDS             6302..6664
                     /locus_tag="PLU55_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87236.1"
                     /translation="MALPLIPIALGGIGGTLLGGSLMGKKSYTTSTVYEAPYHTYSPS
                     MVYAPQTTMAYTSGAKIFNSPFASASERIGLSPTSEPTLSSLPNSNLGSGLNPNSFIP
                     IILVTGAVIVLSNITKKR"
     gene            6669..>6974
                     /locus_tag="PLU55_03935"
     CDS             6669..>6974
                     /locus_tag="PLU55_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87237.1"
                     /translation="MTKRKNKIKVKVSNNTTNTLIKVAGLGIVSYLAFKMLSNNENGL
                     AYLGGSSSGYTGSGTDFSGLGLSDFLPFMQETPPTFVVNNPPLPTGFFTPSDEGLNTP
                     "
BASE COUNT         2864 a          748 c         1117 g         2245 t
ORIGIN      
        1 tatataaacc ttttggtata tagtgaaagt ataaaggata acaataagct atataaagga
       61 ttaatggata taaaaaaatg gtgtttatta tgaacgctaa agaactaata gaaaaattaa
      121 gaagggctag acccaaacta gctctttatt tggaaaacac tagaaaaaga gaaaatgagt
      181 atataatata caatctaaag gacatagacc agaaggtata tcatactttt aaggctaaag
      241 gattttttta tgccatacaa tacgctaatg agtgggcaaa aaataatcat gctgtaataa
      301 ctggaactac tctttttgat tgtaaggatt atataaacag aactatagag tatagttgcg
      361 ggtgttgtaa aaatgactaa atttttttca gataacgaaa ctagaaagaa aaaaatatcc
      421 gaaactttaa aaaactattt taaatctgaa aaagggaaag agcataagga aaaaataaaa
      481 aaagcgaaaa tagaatattg gaaaaaaata aagggtgaat agacatggaa aaacagatag
      541 atattagggc atatatagaa aatgccgtcg attttcaact taaaaagaaa gagtatccag
      601 aaaaagagat aaagaaaaaa gaggacatag aaaataaaat tatctatgtt tatgactatc
      661 aaaaaatagt aagggaaaat gaaattaaat ccttctatgt ttttgattgt atagatgaaa
      721 atgaagttcc gtttagtttt ttcggttctg atatattggc tagtcaaata tctgataaca
      781 tactcccaat aaaaactaaa ttaataaaag ttcaaactaa aaataattta gctggatatt
      841 attggagttt ttctagacca taaattattt aaatttttat ttttttattt tttattagga
      901 gttgatggat tgggattttt agataaaatt aaaaaaattt ttgaaagtaa agaaaaaaag
      961 aaagctagaa agattgaaaa aataacaagt gatttatcac gaaaacagaa actaccaaag
     1021 aaagaaagtt ataaatataa agaaagtgtt aaacctattt ctgctaaata tcatcatatt
     1081 gttaaaattt atttcgttag agattatggt gatttagatg atgatataat tgatttaatg
     1141 ataaaatttg ttaaaaaata taaagataga tttattgcaa aggaaataaa taaagctatt
     1201 gattatacta atgaacctaa attaaaagat atgtttgaaa ctgaatttga aacagaatta
     1261 tttattgaaa taataacatt agctagtgaa agaaaacgat taacaataaa tgaactttgg
     1321 caatattcac aagaattttt agatacaaaa gctaaaaatg atggtagtgc aaaagctata
     1381 cttgttggat taactaaaat gggcttggta aaaaatgagt tacaatatta aagttaataa
     1441 aaattttcac tttttaacta aaaacgaaaa aacaacaatg ccaagtcata ttatatgttt
     1501 tgatactgaa acagaagtta ttgataatca acataaatta aaacttgggt gggcttttta
     1561 ctgtaaaata gaaaatgata aaataacaag agaaaataaa tttttcttta caaaaaaaga
     1621 acaattttgg gagtttatta aaaaatatgc ttataaaaat acaaaagttt atatttttgc
     1681 tcataatacc caatttgatt ttatgattgt tgggggtagt gtggagttac agaaacataa
     1741 tttatatatt gataatgatt ttattgataa tggtaatttt attctatcgg taaaagaaaa
     1801 aaataaaata aatgaaaaaa agcattatac aggaacaaat tatatttttt tagatactat
     1861 gaattatgtt aaaaaatcat tatcatcaat aggtgaaatg attggattgg aaaagatgaa
     1921 gattgatttt aagacttgtt cttttgaata tttaaaaaaa tattgttata gagatgttga
     1981 aataatacaa aaatttattt tagagtggat agatttttta aaatccaatg atttaggtaa
     2041 ttttaaatat actttggcta gtcaaagttt tagcacttat agacatagat ttatgaatag
     2101 tgatattgga atacataata taataaatgc tattgaacta gaaagaaaga gttataaggg
     2161 tggcagaaat gaagttttta agtttggtaa ggattttgcc tatatttatg atgttaattc
     2221 tatgtatcct tatgttatgg ctaacaatag atacccaact aaaattatcg gtttttatcg
     2281 taacggtaat aaaagttcta ttgaaagggc ttttaataaa ggatttggaa ttatagcaga
     2341 tgttgatatt tcaacaacag aaaacataat aggaataaaa gacgaaagat tattttttcc
     2401 aatagggcaa tttagatgtc aaataacaac acctgaaata aagtatctat tgaataatgg
     2461 acacaaaata ttaaaagttc ataattatgc aatttatgaa atggattata tttttaaaga
     2521 ttttattgaa tttttttata atgaaagatt aaaagctaga aatgagaata atttaattaa
     2581 atctgaaatg tataaaatat taatgaattc tctttatggt aaattcggac agaaaataaa
     2641 ttcttggcta gatattggtg aatatgatat taaaaatgat actgatagga tagaacaaat
     2701 ttataatgtt gatacaaaga aatatacatt attaaaatat ttcggtggta aaatctataa
     2761 aaatgatggc tatgttgagg gctataatag ttttgttgct attcctagtt ttgtaactgc
     2821 atattcaaga gtttatcttt ttgaacttat gaaaattgca ggattagaaa atgtcttata
     2881 ttgtgatact gatagcttat ttttaactgt tgatggtcat aacaatatta aaaatagtcc
     2941 ttatgttgat gataaaaagc taggttgcct gaagttagag aaaaaaggaa atgttaatat
     3001 ttttgggtgc aaagactata attttaataa ggaaagaaaa acaaaaggtg ttactaaaaa
     3061 tgctaaagtc ttggataaaa acaagtttga atatacccaa tttacaaaat taagaactgc
     3121 tttaaggaaa aatcttttag atggagttta tattaacaat attgagaaaa ctcttaaaca
     3181 agaatataaa aaagcaatag taaaagataa taaaactact cctttccgat tagatttata
     3241 aataaaaaag acataaaaaa tttatggtaa ttatagaaaa agacataaat aaaaatatag
     3301 aaactgaaaa agacataaaa aaattgaagc gaactgataa aggtgtttgc gctagttgta
     3361 agcgccatag aattataaaa gattttgatt tatatctatg taaaacttgt ttaaatgcta
     3421 aaaaatattg gataggattt aatgaaaaaa agattaataa taatatcgat aatgatattt
     3481 ctgtttctaa agagattgaa acagaagcta aaatagaacc aatatcttat ttttgcgatt
     3541 attgtaataa taagatagct tatgcttcaa aaaaatgtaa taaatgcggt aatattttaa
     3601 attggctggg aactaaatta gaatatgata ctaattattt aatatgcggt tcttgtggta
     3661 tgatattaga atttaatgcc aataaatgcg aaaggtgttt ataaaatggt aaaagaaata
     3721 gttaatattc ctaatgaaat ttctctaata cctcaatcta atcaacctat aatatccaat
     3781 ccaatgggaa tagctcaatc tggaataagt gatattgaaa actatattaa attaattgaa
     3841 aaagctatag atttaattga taaattaaaa tctttatctg gaattaaaac agaaactaac
     3901 actataataa ataatactcc tcacccatca gtatcagata tgccaagact aaacactaat
     3961 tcaaataatg aaatcataga taatgatata gaaggtgaaa aaatgaacat aaatatagat
     4021 ttaatattaa atattttaaa acagattgaa gcatttaaaa aagatatgac tataacagaa
     4081 ttaataaaaa taatagaaaa tgataaagat acaatccaaa aaatcataaa cgctaatatg
     4141 gatatgatta aatgagtaaa agaataacta tagtaggact tcagggttct ggaaagacgc
     4201 agttagctaa aaaaatgata agggataaag taagaaaaaa taaaaaagtt ttagtgttcg
     4261 atattttagg cgaatatgta aaagataaat cgttaggcaa tatagatgtt tttattcccc
     4321 aagatagaat agaaccctta ttagaaattg atacagttct taaaaattat gttgttgaac
     4381 cgtatattta tgataagaaa aagaattatt cattaattgt ttttgatgag gcttcaagat
     4441 attttaacaa taaacttaag ttacccagta atatgggatt tataaatgat atggctagac
     4501 atattgaatt agatattata tgcatagcta ggcgttttac ccagttaaat actgatatag
     4561 ttgaattatc tcattatctt tatattttta atcaggcagg aataaacgat attaaaagat
     4621 taaatgaatt atctatagga ttgggtgata ctgttttaac attaaataag tatcattata
     4681 tagaagtcaa tcaatttagg caatattcaa tcaaaaaacc gataaaaata atggattagc
     4741 aatagattta tatactcttt taattatacg caaatatgca atataaaatt attgcaaaaa
     4801 gtaaggtgaa aatatggcag ttttaggaaa acaggttagt ggttccgttg attatataga
     4861 tttattcacc gctggaatat ttaaatttta ttccgaaaaa gcacttcaat caatgatagg
     4921 caacggaact atgatgtcag gcgccattaa actaggcgca ggacttatgt ctagaaagtt
     4981 tttaggacgg ggtattctag gcgacgccgt aagtctaggc tttgctatag atggcgttga
     5041 agatatacta tttggattac atggtggttt acccagaaat attgaaggtg ggtggtaagt
     5101 atggttgata ctcaaatggt aggtaagttt agattggttc actatgacta taacgataca
     5161 gctctagccg ttctaactga aaaacacgca tatgagttcg ctaaaggcaa tacagcacca
     5221 actgacaggc aaacacaacc atcaataaac attaatgaca gcactataat aaaagaggac
     5281 gataagttag tagtgttagt taatctagat gctaatatta ctgaacacag cgctagttca
     5341 caaagaaatt tatggacatg gcaaatacct ataactttta aaaacgaaag aacaggtaat
     5401 aagtttaaaa agactttatc tgttaatgac ttcagtttta caggtgcaga agcgccagcc
     5461 aactctaaag cttgggtatc aggcaaatgg tatatgttag gctattatca ggttccagcg
     5521 caatcatcaa taaagttagg acatactata caagatgtta gagttgatag taaaataata
     5581 ttacaccaag ctctcacaac ttcgtaaaca ttatatttta ttttttaaat ttatttaatt
     5641 aaaaaataaa gttgataaaa atgacagata aatggactta tggaaaaagt aaaaaatacg
     5701 atatatatac tccttataga taccctatta atagagttcc ccccgaaaat agaagtttct
     5761 tatctggatt taaacaggtt attgaaatcc cagcattaat ctctaaatct tttgctcaat
     5821 atgataaaga aaattcagaa attataaaaa tgggtgttaa aagatatggt tggtttaatt
     5881 ccttcaccgt tcttaatcaa tctgaccaga tagttgaaat ttcattagat tatagtaata
     5941 ataaaaaata tgtaattcca gcaggagttc aaacttcaat atcagatgtc ttatttgaaa
     6001 gcttcaatat taaaaatata ggaaacggaa atatagcttc agacggatta gtaaaagtct
     6061 atgtcatata tgaaagacca attataagag aaggtgttta aaaatggatt ataaaaaatt
     6121 cttaagccgg aaatttatag ctatgataat aggcattatt tgttttatta tgaccacttt
     6181 aatgccagaa cttgattatc aaagtctaga aaatataatt tatataataa tggcttatat
     6241 atttgcggaa gctggtatag acgccgtagc ggttcataga aaaaatgggg gggaataaaa
     6301 aatggcatta cctttaatac caattgctct aggtggtata ggcggaacgc ttttaggcgg
     6361 ttctttaatg ggaaaaaaat cttatacaac ttctacagtt tatgaagcac cttatcacac
     6421 ttattcacca tctatggttt atgcgcctca aacaactatg gcttatactt ctggcgcaaa
     6481 aatatttaat tcaccgttcg ctagtgctag tgaacggata ggtttatcgc caacttcaga
     6541 acctactcta tcatcgttgc caaattcaaa tctaggcagt ggattaaatc caaacagctt
     6601 tattccaata atattagtaa ctggtgctgt tatagtatta tcaaatatca ctaaaaaaag
     6661 gtgattaaat gactaaaaga aaaaataaga taaaagttaa agtttccaat aataccacta
     6721 acactttaat aaaagttgca ggattaggta tagttagtta tctagctttt aaaatgttat
     6781 ctaataatga aaatggatta gcttatctag gtggttcgag tagtggttat actggttctg
     6841 gaactgattt tagtggtttg ggtttatctg actttttacc gtttatgcag gaaactccgc
     6901 caacttttgt agtcaataat ccgccattac caacaggatt ttttactcct tctgatgagg
     6961 gattaaatac tcct
//
LOCUS       DAOPCH010000045         6836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_9911_length_6836_cov_5.510987, whole genome shotgun sequence.
ACCESSION   DAOPCH010000045 DAOPCH010000000
VERSION     DAOPCH010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9911_length_6836_cov_5.510987"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..2594)
                     /locus_tag="PLU55_03940"
     CDS             complement(63..2594)
                     /locus_tag="PLU55_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HPJ87238.1"
                     /translation="MGDDFFGEYEVVSKCHKCPLKKNSHCYNDKIMMINKGYDVLFVL
                     ESNEPSNRLQYFINKVDRIFTSYSIINGINCSPKNGEMPSPIYKIYRHCNNITEELAS
                     KYKVVVTIGSGLQAITQSSDINTYEDFTEFLFNQTYFYTRYDWKKKTRVYPIPRITDW
                     QGQDNFRSFFVNKQFTFVKEYLKNYSEFQFEDYNCIKIENCDEFLEQNNDKEYVAWDT
                     ETNNLNCFVDDFKIGCLTLSFDGKNGYYLPFDKIDKNKLNLFFKNKKGIGANLKYDIK
                     AMSRYDIDNCVVDEDVILLYHLLNTVRETNSLKSLAWLIGFGGYDTDLDDFIQKYKIK
                     NYLEIPEDLLYKYACIDAIVAYRLFDYAKENLIKKQSQVYKLYKETVIPVIPAFIEME
                     INGLLVNMKYLNEYHNKIQEQIKELEIEIKKEIGKDVEISSNEQLGKALEKLGLPDLG
                     RTKKGHFQTGADILQQWINLGFDIVKKIEKYRALCKLDDTFVGDIEKRNGITQYIMSD
                     KRVHGTFSPAMTSSMRASSNSPNLQNFPHYNEEGKTFRKIFTCPENYYIAEADFAGFQ
                     LRIACIESDDENMKNVFLNLGGDMHSFSAKNIFCKDITLEEFLEKKGEEPYKTLRYKA
                     KNQINFPLLFSSSSYVIYGTIRDEWSEKERLDYIEENNLTISLNRDEQPDTVLTVIND
                     IHKKFFETYSGLRDWIKMNLETTLSLGYNDSLFGGRRHLPQLLYKGETFDRSEWTNLC
                     NIATNSRIQNFEAITAYKVLTKIHSEMKRRRLKSKLVGAVHDSIVFYIHKDEVEIMYN
                     LTLKYMNNTKDYIIPILGDFSLGEIWGFSKEITAEDLPKFRMATN"
     gene            complement(2607..2840)
                     /locus_tag="PLU55_03945"
     CDS             complement(2607..2840)
                     /locus_tag="PLU55_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87239.1"
                     /translation="MAKIKFEWLSPEQKREKSLGIVKKFQKEINSLQNKIMKVDRTEE
                     TIKIYFSDTESITISGWIAGQIVVKDTTIESWF"
     gene            complement(2853..3455)
                     /gene="thyX"
                     /locus_tag="PLU55_03950"
     CDS             complement(2853..3455)
                     /gene="thyX"
                     /locus_tag="PLU55_03950"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPJ87240.1"
                     /translation="MSFNVEIIAVTPNALDVIEKAGRLCYNSQMNKETQDKFIADKVK
                     KGHESILEHASVTFEIDGVSRALTHQLVRHRIGCSYSQQSQRFVKSDNFDYVIPEDIR
                     SNSEIFSDYFNLMENIKSFYSKAVGKGIPAENARFMLPNATTTKIMLTMNFRALRHFI
                     ELRADKHAQWEIREVAKEILLLMCKDYKCCFDDLAEKYIF"
     gene            complement(3469..3981)
                     /locus_tag="PLU55_03955"
     CDS             complement(3469..3981)
                     /locus_tag="PLU55_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87241.1"
                     /translation="MKLLLSLLTLFFISASFPQEVKEKENSLLTYAQTKMSINNYVDM
                     VRALEIGERYFKTDKDIVLAVLYIESDFNVFAYNQETNDYGLSQQNGKYILDRYTIVK
                     EILDKERIECNINHRFNIYLNVLACYYYLDHCIELKGSKSQGIKKYNGIGVRAELYMY
                     KVIDIYFKNV"
     gene            complement(3978..4151)
                     /locus_tag="PLU55_03960"
     CDS             complement(3978..4151)
                     /locus_tag="PLU55_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87242.1"
                     /translation="MTNDEKIKNMSIDERAVFFAKYMSCELCENNYTPENKCINDDMC
                     VLKMKKYLESEAE"
     gene            complement(4148..4303)
                     /locus_tag="PLU55_03965"
     CDS             complement(4148..4303)
                     /locus_tag="PLU55_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87243.1"
                     /translation="MTEEEIEKEVFLLDKFREKLLNDKTRADAFFQRIGIYNSEGKLT
                     KEFGGEE"
     gene            complement(4296..4541)
                     /locus_tag="PLU55_03970"
     CDS             complement(4296..4541)
                     /locus_tag="PLU55_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87244.1"
                     /translation="MSSLTRQNRSRPIKWFAKKFQWDCVDFLNSEWFDEEDKKTVLPE
                     LEYLRNFIENDNSGVRFRTNEKDLAYKYIMQGFKKID"
     gene            complement(4538..4969)
                     /locus_tag="PLU55_03975"
     CDS             complement(4538..4969)
                     /locus_tag="PLU55_03975"
                     /inference="COORDINATES: protein motif:HMM:NF020715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1937 family protein"
                     /protein_id="HPJ87245.1"
                     /translation="MANNSYLVMFVKNIIPEVTTRIGRVANNNRRIKMNKKKKHIYIA
                     SSYSKGDGFVNTQRQIDCANKLIDMGYIPFSPLLMSVYLHAQIERDWQTWMDIDYSWV
                     ERCDGLIRLEGESKGADAEVEYAKKFGIPVFHSLEEIRFEA"
     gene            complement(4970..5596)
                     /locus_tag="PLU55_03980"
     CDS             complement(4970..5596)
                     /locus_tag="PLU55_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87246.1"
                     /translation="MAKKVKPKIILISGKKQSGKNLTASLIEELSENPNNIEVFAFAD
                     TLKNMASNFFYHVMGNEYYVEHKEYEKDSFKNKVIPFRFNEKKCTYRKFLQYFGTDFM
                     RELFSDNLLVDIVIEFIKGNLLHTADNMFVITDARFPNEIERVQEKLGKKCDIISLRV
                     ERPSLPVNSNAHISETALDDYFFDYTIINDGTKKELKEKVKDFVDGLN"
     gene            complement(5596..6075)
                     /gene="dut"
                     /locus_tag="PLU55_03985"
     CDS             complement(5596..6075)
                     /gene="dut"
                     /locus_tag="PLU55_03985"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPJ87247.1"
                     /translation="MLCLLVITLIFYKDGVKKVKIKKLNEDAKLPEYAHKGDAGLDLF
                     STIDVEIPSGETRLISTGIVIALPFRTEAQIRPRSGLALKNSITVLNTPGTIDSGYRG
                     EIGVILINHGKEVFKVTKGMKIAQMVIAQYNIAELQEVIELDNTKRAEKGFGSTGVN"
     gene            complement(6136..6303)
                     /locus_tag="PLU55_03990"
     CDS             complement(6136..6303)
                     /locus_tag="PLU55_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87248.1"
                     /translation="MVYKDMTFCEYKECVKFEQCIRALTKKVEEDAERWWGNKNAPFS
                     VFSEKPDCFKN"
     gene            complement(6409..>6836)
                     /locus_tag="PLU55_03995"
     CDS             complement(6409..>6836)
                     /locus_tag="PLU55_03995"
                     /inference="COORDINATES: protein motif:HMM:NF018627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87249.1"
                     /translation="KEIVKAIYQIYDGDTNYQEVLANFNAINTLHSLHNDGHYITILT
                     SGFGKSNIVKQKIEWLERYLPFLSIKNIAFVGNKNITFGDVIIDDAWHNIVNAKYDCK
                     ILIPYEWNRKEFEKSGIDIIRTDDWNRIYTICNDFSLKK"
BASE COUNT         2204 a         1206 c          703 g         2723 t
ORIGIN      
        1 atcttacttc tgttggtttt gccattattg acaatctcct tttaaaaata tatttctact
       61 ttttaattag tagccattct aaattttggt aagtcttctg ccgttatttc tttactaaat
      121 ccccatatct ctccaagtga aaaatctcca agaatgggaa taatataatc cttagtatta
      181 ttcatatatt tcagtgtcaa attatacatt atttccactt catctttatg tatataaaaa
      241 actatagaat catgtactgc accaacaagt ttacttttta accttcttct tttcatttca
      301 ctatgaattt tagtaagaac tttataagcg gttatagctt caaaattctg aatccttgaa
      361 tttgtagcaa tattgcacaa attagtccat tcacttctat caaaggtttc acctttatat
      421 agtaattgag gtaaatgtct tcttccacca aacaaactat cattatatcc aagagataat
      481 gtagtttcta aattcatttt aatccaatct cttaatcctg aataagtttc aaaaaacttt
      541 ttgtgtatat cattaataac tgttaaaact gtatcaggtt gttcatctct atttaaagat
      601 attgttaaat tattttcttc aatgtaatca agtctttctt tctctgacca ttcatcacgt
      661 atagtaccat aaataacata ggaagaactt gaaaataata aaggaaagtt aatctgattt
      721 tttgctttat accttaaagt tttataaggt tcttctcctt ttttttctaa aaattcttca
      781 agtgttatat ctttacagaa aatatttttt gcggagaaac tgtgcatatc cccaccaaga
      841 ttaagaaaaa catttttcat gttttcatca tcactttcaa tacaagcaat tcttaattga
      901 aaaccggcaa agtctgcttc tgctatatag taattctcag ggcaagtaaa tatttttcta
      961 aatgttttac cttcctcgtt gtagtgtgga aaattttgca aatttggaga atttgaactt
     1021 gcacgcatag aagaagtcat tgcaggagaa aatgtaccat gtaccctttt atccgacatt
     1081 atatattgag taataccatt tcttttttct atatcaccaa caaaagtatc atcaagttta
     1141 cacaatgctc tatacttttc aattttcttt acaatatcaa accctaaatt tatccactgc
     1201 tgaagtatat ctgcacctgt ttgaaaatga cccttcttag ttctacctaa atctggaagt
     1261 cctaattttt caagtgcttt tcctaattgt tcattagaac ttatttctac atcttttcct
     1321 atttcttttt ttatttctat ttcaagttct tttatttgtt cttgtatttt attatgatac
     1381 tcattcaaat atttcatatt gactaatagc ccgtttattt ccatttcaat aaatgccgga
     1441 attacaggaa taactgtttc tttataaagt ttatatactt gtgattgttt cttaattaga
     1501 ttttctttag cataatcaaa tagtctataa gctactattg catctataca agcatattta
     1561 tatagaaggt cttcaggaat ttctaaataa tttttaattt tatatttttg aataaaatca
     1621 tctaaatctg tatcatagcc accaaaacct attaaccaag ctaatgattt taatgaatta
     1681 gtttccctta ctgtatttaa taaatgataa agcaatatca catcttcatc tactacacaa
     1741 ttatcaatat cataccttga cattgcttta atatcatatt ttaaattagc acctattcct
     1801 tttttatttt taaaaaataa atttaattta tttttatcta ttttatcaaa aggaaggtaa
     1861 tacccatttt ttccatcaaa agaaagtgtt aaacaaccaa ttttaaaatc atctacaaaa
     1921 caatttagat tatttgtttc agtatcccat gctacatatt ctttatcatt attttgttct
     1981 aaaaattcat cacaattttc aatcttaata caattataat cttcaaattg aaattcagag
     2041 taattcttta aatactcttt aacaaaagta aattgtttat taacaaaaaa acttctgaaa
     2101 ttatcttgac cttgccaatc tgtaattctt ggaataggat aaactcttgt tttctttttc
     2161 caatcatatc ttgtataaaa ataagtttga ttaaataaaa actctgtaaa atcctcataa
     2221 gtgtttatat ctgaactttg tgtaattgct tgtaatccac tacctattgt aacaacaact
     2281 ttatacttgc ttgcgagttc ttctgtgata ttattacaat gtcgatatat tttgtaaata
     2341 ggtgaaggca tttcaccatt tttaggtgaa caatttattc cattaataat agagtagctt
     2401 gtaaatatcc tatctacttt atttatgaaa tactgtaatc tgtttgaagg ttcatttgac
     2461 tccaatacaa ataaaacatc ataaccttta ttaatcatca ttattttatc attataacaa
     2521 tgactatttt tctttaaagg acatttatga catttactta caacttcata ctcaccaaaa
     2581 aaatcatcac ccattaaagc acttccttaa aaccaagatt ctattgttgt atctttaact
     2641 acaatttgac cggcaatcca acctgatatt gttattgatt ctgtatcaga aaaatatatt
     2701 ttaatggtct cttctgttct atccactttc attattttat tttgaagaga attaatttct
     2761 ttttgaaact tcttaacaat acctaaagat ttttctcttt tctgttcagg tgaaagccac
     2821 tcaaatttta tttttgccat tctttcaatt ccttagaata tatatttttc tgctaaatca
     2881 tcaaaacaac atttatagtc tttacacata agtaatagta tttcttttgc aacttctctt
     2941 atttcccact gtgcatgttt atctgctctc aattcaataa aatgcctaag tgctctaaaa
     3001 ttcattgtta acataatttt ggttgttgtt gcattaggta gcataaatct tgcattttcc
     3061 gcaggaatac cttttcccac tgctttagaa taaaaggact taatattctc cataagattg
     3121 aaatagtcag aaaaaatctc agaattactt cttatatctt ctggtattac ataatcaaaa
     3181 ttatctgatt taacaaatct ttgtgactgt tggctgtaag aacaaccaat tctatgtctt
     3241 actaattgat gggtaagcgc acgagaaaca ccatcaattt caaaagtcac tgaagcatgt
     3301 tcaagaattg attcatgtcc ttttttaact ttatcagcaa taaatttatc ctgtgtttct
     3361 ttattcattt gagaattata acaaagtctt ccagcttttt ctataacatc taaagcatta
     3421 ggtgttactg caattatttc tacattaaaa ctcattcaat tttctcctct atacattttt
     3481 aaaatatata tctattacct tatacatata caattcagct cttactccta taccattgta
     3541 ctttttaatg ccttgacttt tagaaccttt taattctata caatggtcaa ggtaataata
     3601 acaagcaaga acattaaggt atatattaaa tctatgattg atattacact ctattctttc
     3661 cttatcaaga atttctttaa caattgtata tctatcaaga atatattttc cgttctgttg
     3721 tgaaagtcca taatcatttg tttcttgatt atatgcaaac acattaaaat ctgattcgat
     3781 atataaaact gcaagaacta tgtctttatc tgttttgaaa tatctttctc ctatttcaag
     3841 tgctcttacc atatcaacat aattattgat agacatttta gtctgtgcat aagtcaaaag
     3901 actattttct ttttctttta cttcctgcgg aaaacttgct gaaataaaaa atagggttaa
     3961 tagcgataat agtaatttca ttctgcctcg gattcaagat attttttcat tttcaataca
     4021 cacatatcat cgttgatgca tttattttcg ggagtataat tattttcaca aagttcacat
     4081 gacatatatt ttgcaaaaaa tacagctctt tcatcaatgc tcatattttt aattttttca
     4141 tcatttgtca ttcttcacca ccaaattctt tagttagctt tccttcagag ttgtatatac
     4201 ctattctctg aaagaaagca tctgctcttg ttttatcatt tagaagtttt tccctaaatt
     4261 tgtcaagcaa aaaaacttct ttttcaattt cttcttcagt caattttttt aaacccttgc
     4321 atgatatatt tgtatgctaa atctttttca ttagttctaa agcgtacacc tgaattatca
     4381 ttctctatga aatttctaag atattctaat tcagggagta ctgtcttttt atcttcttca
     4441 tcaaaccatt cactatttaa aaaatcaaca caatcccatt gaaatttttt agcaaaccat
     4501 tttattggtc ttgacctgtt ctgtcttgtc aaactactca agcttcaaac cttatttctt
     4561 caagactatg aaataccgga ataccaaatt ttttagcata ttcaacttct gcatcagctc
     4621 ctttagattc accttcaagc ctaattaatc cgtcacatct ttctacccat gagtagtcaa
     4681 tatccatcca tgtttgccaa tctctttcaa tttgagcatg aagataaact gacatcaata
     4741 aaggtgaaaa aggaatatac cccatatcta ttagtttgtt agcacagtca atttgtcttt
     4801 gagtgtttac aaatccatcc cccttgctat agctagaagc gatgtatata tgttttttct
     4861 ttttattcat tttaattctc ctattattat ttgcaactct tccaatcctt gttgtaactt
     4921 caggaattat attcttaaca aacatgacaa gatatgaatt gtttgccatt cagtttaacc
     4981 catctacaaa atcttttact ttttctttca attctttctt agtaccatcg tttattatag
     5041 tataatcaaa aaaataatca tctaaggctg tttcagaaat atgtgcatta gaattaacag
     5101 gaagagaagg tctttctact cttaaagaaa taatatcaca tttctttcct aatttttcct
     5161 gaactctttc aatctcattt ggaaatcttg catctgttat aacaaacata ttatcagcag
     5221 tatgaagcaa attaccttta ataaattcaa taacaatatc caccaataga ttatctgaaa
     5281 aaagttctct cataaaatca gtaccaaaat actgaagaaa ttttctataa gtacatttct
     5341 tttcattaaa tctaaaaggt attactttat ttttgaaact atccttttca tactccttat
     5401 gttctacata atattcattt cccataacat gatagaaaaa attagaagcc atgtttttta
     5461 aagtatcagc aaaagcaaaa acttcaatat tattaggatt ttcactaagc tcttctatca
     5521 aagaagctgt caagttttta cccgattgtt tctttccact tatcaatatg atttttggtt
     5581 ttactttttt agccattaat ttactcctgt tgaaccaaag cctttttcag ctctttttgt
     5641 attgtcaagt tcaattactt cttgaagttc agcaatatta tattgtgcaa taaccatctg
     5701 tgcaatcttc ataccctttg ttactttaaa aacttcttta ccatgattaa taagtataac
     5761 acctatttcc cctctgtaac ctgaatcaat agtcccaggt gtatttagaa cagttatact
     5821 gtttttaagt gcaagaccac ttctaggtct tatctgtgct tctgttctaa aaggaagagc
     5881 tataactatt cctgttgaaa ttagtcttgt ttcacctgaa ggaatctcta catctattgt
     5941 agaaaataaa tccagtccag catctccttt atgtgcatat tccggtaatt tagcatcttc
     6001 atttaatttt ttaattttaa ctttttttac accgtcttta taaaaaataa gtgtaatcac
     6061 aagcaaacaa agcataatca aaaaattaaa tacttcctgt gataaaataa acataatcta
     6121 ttctcctttt taattctaat ttttgaaaca atccggtttt tctgaaaaaa cactaaaagg
     6181 tgcattcttg tttccccacc atctttcagc atcttcttca acttttttag ttagtgctct
     6241 tatacactgt tcaaatttga cacattcctt atattcacaa aatgtcatat ccttatatac
     6301 catctttttc tcctttacta ttttttctgt tatacttttt cttactctga attatttttg
     6361 ggcaaggaac tttcagccgt cctgtcctta ttgttttctt cttctttttc atttctttaa
     6421 actaaaatca ttacaaatag tataaattct attccaatca tctgttctaa ttatatctat
     6481 tccacttttt tcaaactctt tacgattcca ttcataagga ataagtattt tgcaatcata
     6541 tttagcgttt actatattat gccaagcatc atctataata acatctccaa aagtaatatt
     6601 cttattacca acaaaagcaa tattttttat tgaaagaaat ggtaaatacc tttccaacca
     6661 ctcaattttt tgttttacta tattagactt accaaatcca gaagtcaaaa ttgtgatgta
     6721 gtgcccatcg ttatgaagag aatgtagggt attaatagca ttaaaattag ctaatacctc
     6781 ttgataatta gtatccccat catagatttg ataaatagct ttaactattt cttttg
//
LOCUS       DAOPCH010000046         6717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_10125_length_6717_cov_5.471780, whole genome shotgun sequence.
ACCESSION   DAOPCH010000046 DAOPCH010000000
VERSION     DAOPCH010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10125_length_6717_cov_5.471780"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..903
                     /locus_tag="PLU55_04000"
     CDS             196..903
                     /locus_tag="PLU55_04000"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: protein motif:HMM:NF003075.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HPJ87250.1"
                     /translation="MDIQHQAMGYDRTATMFSPEGHLLQVEYAEKTVRLGSSSIGMVC
                     SDGVFILADKRTEDKLVVKGSVNKISEVDSHIIASSAGIFSDARILIERAQLFSQQHR
                     VTYNSPIEPELVIKEIANMKQQFTQYGGARPFGVSMMIAGINGKKGEVFVSDVTGNYF
                     SYYTNVIGENDDKIKEELREKYKQDLSIKQGVKLALEIFEKVQGSKFNIKKFELAYIP
                     KETAKLKKLEGESIKEL"
     gene            910..1581
                     /locus_tag="PLU55_04005"
     CDS             910..1581
                     /locus_tag="PLU55_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HPJ87251.1"
                     /translation="MTNTTARIKKTGKNFEIIVDLDDALKFKKGLSEFIQAETEFIFK
                     DMKKGERASEKELKDAFGTTDTHEIVKKIVKEGEILTTQEYRDAEQEKKFKQVVDFLT
                     KNSYDPQTGRPHTAERIKNALEQANVNIKNTSIENQINEILEKISLLIPIKIETKKVK
                     IKIPALHTGKAYGVVATYKTSENWLNDGSLEVIVEVPSGMIMDFYDKLNSVTRGSAIT
                     NEIKE"
     gene            1583..2311
                     /gene="rrp4"
                     /locus_tag="PLU55_04010"
     CDS             1583..2311
                     /gene="rrp4"
                     /locus_tag="PLU55_04010"
                     /inference="COORDINATES: protein motif:HMM:NF003181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Rrp4"
                     /protein_id="HPJ87252.1"
                     /translation="MTEEHKRTVVIPGETIKEGDAYLPGEGTEKQGNKIVALRYGLAE
                     EHNRLVKVIPLSGVYQPRRGNIVIGKCNLITFNGWVIDIGTAENAFLPIMETPRYVAK
                     DRLDEVMKIGDICVVQIFSVNGRGIDLTMKARGLGRIDEGIVFRINSNKVPRVIGKEG
                     SMIKIIRDYTNCNITVGQNGYIWIKGDKIEDELFAKKAIMFITEKSFMEGLTEKVEEW
                     FKENGGVKKETKKSEEKSGDKKED"
     gene            2313..3029
                     /locus_tag="PLU55_04015"
     CDS             2313..3029
                     /locus_tag="PLU55_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030792.1"
                     /note="involved in the 3' to 5' degradation of a variety
                     of RNA species; forms a trimer of heterodimers (hexamer)
                     with Rrp42; Rrp41 is the catalytically active subunit;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex exonuclease Rrp41"
                     /protein_id="HPJ87253.1"
                     /translation="MTSNFKRPDGRKFDELREFEAKVGIVPNADGSAMYRSGETIAIA
                     AVYGPKKMHPQHSQNPEKGTLRCTYNMNSFSVSERIRPGPSRRSTEISKITEWALEPV
                     LMLDKFQNMVVDVHINIIQADAGTRCAGINAAAMALAHAGIPMKDMVSSVSVGKLDKT
                     IVVDLSKEEEDYEEGEGPTDIPISMTHSGDITHFQLDGKIDSKQLMEAVELAKKSCEQ
                     IYEVQKKALKDSIDLTGGED"
     gene            3031..3843
                     /gene="rrp42"
                     /locus_tag="PLU55_04020"
     CDS             3031..3843
                     /gene="rrp42"
                     /locus_tag="PLU55_04020"
                     /inference="COORDINATES: protein motif:HMM:NF003282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex protein Rrp42"
                     /protein_id="HPJ87254.1"
                     /translation="MGGEEIDITTKVTAERIKKYLEQGKRFDGRKTDEFRNIIVEPNV
                     SNKAEGSVKVKVGKTEVIVGVKMETATPYPDSPNKGNLMVTAEFLPLSSSRIELGPPK
                     FDSIETGRIIDRIIRESKFIELEKLCIKEGEKVWTVFIDIYTINDDGNILDAAGIGAV
                     AALKLAKVPKYDEEKEKVDYKEYTGKLPLKEMNPFTITVHKIGKHLIVDPTREEEDVS
                     EARISVGSSEGIIHSLQKGNSGCFSAEEIKKCFEIVEKSWKELFKKVNAQLK"
     gene            3859..4254
                     /locus_tag="PLU55_04025"
     CDS             3859..4254
                     /locus_tag="PLU55_04025"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013303641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HPJ87255.1"
                     /translation="MAPPTETQNFTKYEKARILGARALQLAMNAPLLLKISEEDLQKI
                     NYDVLKIAEVELDSGVLPISIRKPFPEKKEGKLKRDATPKASDKKVESGEDIEEEKMA
                     DESEIMELANPEDEYEGSSDGDGDGEYGE"
     gene            4328..4675
                     /locus_tag="PLU55_04030"
     CDS             4328..4675
                     /locus_tag="PLU55_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87256.1"
                     /translation="MEKPELNEINKLVSMAFGKGFSGEYVMFESRGRQVVIKGNKLDT
                     IARKKFPYIPKLNEKYEIAGRFTSEDGSSMNVDLRYANSAERYADYYYLHFKKNPLIN
                     GEEVSIKFKGLKV"
     gene            4712..5890
                     /locus_tag="PLU55_04035"
     CDS             4712..5890
                     /locus_tag="PLU55_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87257.1"
                     /translation="MVVIREVSAKSIFDSRKEKTILVSIKTNSGKTFKASSPSGKSTG
                     KYEVHCYKKSLEDDIKTIKQFKEYFSEEILDEYEDLKRVEDILDGHIGGNTLFAFESA
                     VLKAIADEKKKQIWEIVNPTLREEKEKKFPRLVGNCIGGGAHSEVKGKKPDFQEFLLI
                     PEGNSPEKSFKINKEAKEKAGFILKKKDKLFKSQKNDEDAWITSLNEKEVLDILKELD
                     VPIGLDIASSGFYHRGKYHYENPMLKREIDEQLTYVGNLIKNYNILYVEDPIQEEDFE
                     SFSKLLKKCPNSLIVGDDLTVTNSKRLKKAIEMKSINAIIVKPNQCGSLIEVKKVCEI
                     AKENNIKMIFSHRSGETNETILSDLAFGFQADFLKCGITGKERECKIKRLIEIEKSLK
                     "
     gene            6072..>6717
                     /locus_tag="PLU55_04040"
     CDS             6072..>6717
                     /locus_tag="PLU55_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87258.1"
                     /translation="MPRVKKTENATTSKEVDKKKKSEKKEKIADKVSNKEVEEQLEGK
                     SLEKAAKAESLATEEMDEKKKKILEKAAKLKEKLSEEAPSSEELKEKVKIKKRTDMLV
                     PLEEYVKAGIYLGTKVVTPEMKPFVYRRRADGLAIFNTDLIDEKLKEGVEYLCKFNPE
                     DIILVCKRQAGWKAAMKFSELTGIRTFTKKYPAGILTNTALPDFFENELTIIVDS"
BASE COUNT         2830 a          781 c         1281 g         1825 t
ORIGIN      
        1 ccatgagaga atggaggtct tgaaggttag tggaactttg aaaggtttaa gcagataaaa
       61 tacaaagaat cctgtttaaa ttaataaaaa tgttcaccaa aaaccttaaa aacttctaaa
      121 aatgttaaat ttgaacagga atagagaaaa aactaaataa ttataaaatt ctcttgatat
      181 agaatggata tgcctatgga catacaacat caagcaatgg gttatgatag aacagctact
      241 atgttttctc cagaaggaca tttgcttcaa gttgaatatg ctgaaaaaac agtaagactt
      301 ggaagctcaa gtatagggat ggtttgttca gatggagtgt ttatactggc agataaaaga
      361 acagaagaca aacttgttgt taaagggtct gttaataaaa tatctgaagt tgattctcac
      421 attatagcta gttctgcagg aatattttct gatgcaagaa tactcataga aagagctcag
      481 cttttctcac agcaacacag agtaacatat aattctccta tagaacctga attagtcata
      541 aaagagattg caaatatgaa acagcaattc actcaatatg gaggagcaag accttttgga
      601 gtttcaatga tgattgcagg gataaatgga aagaaaggag aagtttttgt gagtgatgta
      661 acaggaaatt atttctcata ttatacaaat gtaattggag agaatgatga taaaataaaa
      721 gaagaattaa gagaaaaata caaacaagac ctctctataa aacaaggggt taaacttgca
      781 cttgaaatat ttgagaaagt gcagggaagt aaatttaata ttaaaaaatt tgaattagca
      841 tatattccaa aagaaactgc aaaattaaag aaactggaag gggaaagcat taaagaatta
      901 taaaatcaaa tgactaatac aactgcaaga ataaaaaaaa caggaaagaa ttttgagata
      961 atagttgatt tggatgatgc gttaaaattc aaaaaaggtt taagtgaatt tattcaagca
     1021 gaaactgaat ttatattcaa agatatgaag aaaggtgaaa gggcttctga aaaagaactt
     1081 aaagatgcat ttggaacaac agatactcat gaaattgtaa aaaagattgt aaaagaagga
     1141 gagatactga caacacaaga gtatagagat gctgaacagg aaaaaaagtt caagcaggtt
     1201 gttgattttt taactaaaaa ttcctatgat cctcaaacag gaagacctca tacagccgaa
     1261 aggattaaaa atgctcttga acaagcaaat gttaatatca agaatacatc aatagaaaat
     1321 caaattaacg aaattcttga aaagataagt ttattgattc caatcaaaat tgagacaaaa
     1381 aaagtcaaga taaaaatccc tgcattacac acaggaaaag cttatggagt tgtcgcaact
     1441 tataagacct cggaaaactg gttaaatgat gggagtcttg aagttattgt agaagtccca
     1501 tctggaatga ttatggactt ttatgataaa ttaaattcag taacgcgcgg aagtgcaata
     1561 acaaatgaaa taaaagaata aaatgacaga agaacataaa agaacagttg taatacccgg
     1621 agagacaatc aaagaaggag atgcatattt acctggagaa ggaactgaga aacaaggaaa
     1681 taagatagtt gctttaagat atggtttagc tgaagagcat aacagacttg taaaagtaat
     1741 tccactttct ggagtttatc aaccaagaag agggaatatt gttataggaa aatgtaattt
     1801 aataacattc aatggatggg tgattgatat tggaactgct gaaaatgctt tcttgccaat
     1861 aatggaaact ccaagatatg ttgctaaaga cagattagat gaagttatga aaataggaga
     1921 tatttgcgtt gttcagattt tctcagtaaa tggaagagga attgatttaa ctatgaaagc
     1981 aagaggactt ggaagaatag acgaaggaat tgttttcaga ataaactcaa ataaagttcc
     2041 aagagttatt ggaaaagaag gaagtatgat aaaaataata agagattata ctaactgcaa
     2101 tataacagtc ggacagaatg gatatatttg gattaaagga gataaaatag aagatgaatt
     2161 atttgcaaaa aaagctataa tgtttataac tgaaaaatca ttcatggaag gattaactga
     2221 aaaagttgaa gaatggttca aggaaaatgg aggtgtgaaa aaagaaacta aaaaatctga
     2281 agaaaaatca ggagataaga aagaagatta agatgacaag taattttaaa agaccagatg
     2341 gaaggaaatt tgatgagctt agagaatttg aggctaaagt aggaatagtt ccaaatgcag
     2401 atgggagtgc aatgtatagg tcaggtgaaa caattgcaat agcggctgtt tatggtccaa
     2461 agaaaatgca tcctcaacac tcacagaatc ctgaaaaagg aactttaagg tgcacttata
     2521 atatgaatag tttctctgtt tctgaaagaa taagacccgg accgtcgaga agaagcacag
     2581 aaatttctaa aataacagag tgggcattag aacctgtttt aatgcttgac aaatttcaga
     2641 atatggttgt tgatgttcat ataaatataa ttcaggcaga tgctgggaca agatgtgcag
     2701 gaataaatgc tgctgcaatg gctcttgctc atgctggaat acctatgaaa gatatggtat
     2761 caagtgtttc tgttggaaaa ttagacaaaa caattgttgt tgatttaagt aaagaggaag
     2821 aagattatga agaaggtgaa ggacctacag acattccaat ttcaatgaca cactctggag
     2881 atataactca ctttcaatta gatggaaaaa tagattctaa acaattaatg gaagctgttg
     2941 agcttgcaaa aaaatcatgt gaacagattt atgaagttca aaaaaaagct cttaaagatt
     3001 caattgattt aacaggagga gaagattaaa atgggcggag aagaaataga cataacaact
     3061 aaagtgactg ctgaaagaat aaaaaaatat ctagagcagg gaaaaagatt tgacggaaga
     3121 aaaacagatg aattcagaaa tataattgta gagccaaatg tttctaataa agcagaaggt
     3181 tctgtaaaag ttaaagttgg aaaaacagaa gttattgtcg gagttaaaat ggaaactgct
     3241 actccttatc ctgattctcc aaataaagga aatttaatgg taacagcaga attcttacct
     3301 ctaagttcat caagaataga acttggacca cctaaatttg attcaattga aactggaaga
     3361 ataattgaca gaattataag agaaagtaaa ttcattgaac ttgaaaaatt atgtattaaa
     3421 gaaggagaaa aagtttggac agttttcata gatatttata caataaatga tgatggaaat
     3481 attctagatg ctgctggaat tggtgctgtt gcggccttga aacttgcaaa agttccaaaa
     3541 tacgatgaag aaaaagaaaa agtagattat aaagagtata caggaaaact tcctttaaaa
     3601 gaaatgaacc catttacaat cacagttcat aagataggta aacatcttat tgttgaccca
     3661 acaagagaag aagaagatgt gagtgaagct agaatatctg ttggaagttc agaaggaatt
     3721 atacactctt tacaaaaagg aaattcgggc tgtttcagtg cagaagaaat taaaaaatgc
     3781 tttgaaatag tggaaaaatc ctggaaagaa ttatttaaaa aggttaatgc acaattgaaa
     3841 taataacaaa aagataaaat ggcgcctcca acagaaactc aaaacttcac aaaatatgaa
     3901 aaagcaagaa ttcttggtgc aagagcattg caacttgcta tgaatgctcc tcttttattg
     3961 aagataagtg aagaggattt acaaaaaatt aattatgatg ttcttaagat tgctgaggtt
     4021 gaacttgatt caggagtttt gccaatatca ataaggaaac ctttccctga aaagaaagaa
     4081 ggaaaactaa agagagatgc tacacctaaa gcaagcgata agaaagttga aagtggtgaa
     4141 gatattgaag aagaaaaaat ggcagatgaa agtgagataa tggagcttgc aaatcctgaa
     4201 gatgaatatg aaggttcaag tgatggagat ggtgatggag aatacgggga gtaaactgta
     4261 ttttgtatta attctaaaat agttacaata tcgaaatatt tataaagaat ttacgctttt
     4321 agaaaatatg gaaaaaccag aattaaatga aataaataaa cttgttagca tggcatttgg
     4381 gaaaggattc tcaggtgaat atgttatgtt tgaaagtaga ggaagacaag tagtaataaa
     4441 gggaaataag cttgatacta ttgcaagaaa aaaatttcct tatattccaa agttaaatga
     4501 aaaatatgaa attgcaggaa gatttacatc agaagatggg tcaagtatga atgttgattt
     4561 gcgatatgca aactctgcag aaagatatgc tgattactat tatcttcatt ttaagaagaa
     4621 ccctttaata aatggagaag aggtttcaat aaaatttaaa ggacttaaag tataaacaaa
     4681 accttataaa gaactaattt tttcttaaat tatggttgta ataagagagg tttcggccaa
     4741 gagcattttt gattcaagaa aagaaaaaac aatacttgtt tctataaaaa ctaattcagg
     4801 aaaaacattc aaagcttctt ctccgtctgg aaaatctact ggaaaatatg aagttcattg
     4861 ttataaaaaa agccttgaag atgacataaa gacaataaaa caatttaaag aatatttttc
     4921 tgaagaaata cttgatgaat atgaggattt gaaaagagta gaagatattt tagatggaca
     4981 tataggtgga aataccttat ttgcatttga atctgcagtc ttaaaggcaa ttgcagatga
     5041 aaagaaaaaa caaatatggg agatagttaa tccaacactc agagaagaaa aagaaaagaa
     5101 attcccaaga cttgttggaa attgtatagg gggaggagca cattctgaag taaaaggtaa
     5161 gaaacctgat tttcaggaat ttcttttaat acctgaaggg aattctcctg aaaaatcatt
     5221 taaaataaat aaagaagcaa aagaaaaagc tggatttatt ttgaaaaaga aagataaatt
     5281 attcaaatcc cagaaaaatg atgaagatgc ctggataaca tcactaaatg aaaaagaagt
     5341 tttagatatt ttaaaagaac ttgatgttcc aattggatta gatattgctt cttctggatt
     5401 ttatcataga ggaaaatacc attatgaaaa tcctatgttg aaaagagaaa ttgatgaaca
     5461 attaacttat gttggaaatt taattaagaa ttataatatt ttatatgttg aagaccctat
     5521 tcaggaagaa gattttgaaa gcttttccaa acttctaaaa aaatgtccta attcacttat
     5581 tgtaggagat gacttaactg taacaaactc taagagatta aaaaaagcaa ttgaaatgaa
     5641 atctattaac gctataattg ttaaaccaaa ccaatgcggt tctcttattg aagttaaaaa
     5701 agtttgtgaa attgctaaag aaaataacat aaaaatgata ttctcacaca gaagtggaga
     5761 gacaaatgaa acaattttat ctgatttagc ttttggattt caggctgatt tcttgaaatg
     5821 cggaataact ggaaaagaaa gagaatgcaa aataaaaaga ttaattgaaa ttgaaaagag
     5881 tttgaagtag gaaatgattc ttttaattta atgccttaaa aacaaaaccg aagaactctt
     5941 agatatcact ttgttctatc taaaaacttt aggcaaagtt tttaggcctt acgctttcac
     6001 tgaagctcga cctaaaagtt cttgaaaaac aaaaccgaag aacttttaaa ctaatttttc
     6061 tctaaatcct catgccaaga gtaaaaaaaa ctgaaaatgc aactacatct aaagaagtag
     6121 ataagaagaa aaaatcagaa aagaaagaaa aaatagcaga taaagtttct aataaagaag
     6181 ttgaagaaca gcttgagggc aaatcacttg aaaaagctgc taaagctgaa tctcttgcta
     6241 cagaagaaat ggatgaaaaa aagaaaaaaa ttcttgaaaa agctgctaaa ttaaaagaaa
     6301 aattatctga agaagctcca agctcagaag aacttaaaga aaaagtcaaa atcaaaaaaa
     6361 gaacagatat gcttgtacct cttgaagagt atgttaaagc tggaatttat cttggtacaa
     6421 aagttgttac gcctgaaatg aaaccttttg tttatagaag aagagcagat gggctagcta
     6481 tatttaatac tgatttaatt gatgaaaaat taaaagaagg agttgaatat ctttgtaaat
     6541 tcaatcctga agatataatt cttgtatgta aaagacaagc tggatggaaa gcagcaatga
     6601 aattttcgga attaactgga ataagaactt tcactaaaaa atatcctgct ggaatattaa
     6661 caaacactgc acttcctgat ttttttgaaa atgaattaac aataatagtt gattctt
//
LOCUS       DAOPCH010000047         6394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_10695_length_6394_cov_42.321660, whole genome shotgun
            sequence.
ACCESSION   DAOPCH010000047 DAOPCH010000000
VERSION     DAOPCH010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10695_length_6394_cov_42.321660"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2655)
                     /locus_tag="PLU55_04045"
     CDS             complement(<1..2655)
                     /locus_tag="PLU55_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87259.1"
                     /translation="MAKTTNEVNIVWKVKGQNELASAIRGIGSAIKSIDFDKLSEVTG
                     VASILSSLQSNKFNFDVTTGTGIANSIKQVNNLLDELNQVKELRDAINKQPQTIGYYK
                     SVAQTAVGIPPEMNLKNLTAMQRKLTMEMNKTMDEEIKHTLAGLRSIIISAKETVKGG
                     NLEGQFISPEIREQVNVVTNFSDLFRDALHKNFAKFKGITGMIGESIDSIFRNMSQET
                     RDKVIYSTKQEIAKTKEQLRLAKIELSQSKEPGDILNNALNVQRIEVKLNNLRQLQTQ
                     YSQAVKVLSKQEADARAAGGVMKNVQSAKAKADVTANADDLGKLFKDVLFINNKASAK
                     AVSEAIEQREKDLIDFITKSPVYSMTPEERIKFTGESVAKQQDIIRYRELLGRKQNVV
                     SETEFQAMQAGKKIYEVPANFTPQYLDELKNRIKNYSFTSKNLEGLLTQLDALGKLTE
                     KYPAKEKLFLTDEEAEQVAQYMLDFKSMSKKITSELGKSKTMAVGEATRKLAPEEYTK
                     AVTAAKPAYAMMGEVERADALNNLLLKRKDLESQISAVKKQITGLNEADDKAHIEKLQ
                     KLSQELNLTKVNADSIKVVNNDIKADNKKAEAEKRKARQEEAARIKQDKDEAKKKAAE
                     DKLALKQDAEIKRQNDKEAKERERQQKAENRQRDKEERERQKQQTAEQIQAKRQLNEE
                     EKIAKKDIDLEYDTQRKIVELDEQRQVYAGKRINLTAVLNKKEELLTNNAYLQRDIET
                     RLNALGAQRAKYLQMHNDLLRYDRMLANAVTEEERQQIALEKQQYMQSQNEEMLQLHQ
                     LQMEKQQNDLTLIRCNLLIKTGAAHKENIMATIGHYLSLDKIVSRMSFVWTAMFSYKI
                     MYAIQNGFMEAKNAAMTLNEQLQRMKSIM"
     gene            complement(2666..3943)
                     /locus_tag="PLU55_04050"
     CDS             complement(2666..3943)
                     /locus_tag="PLU55_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87260.1"
                     /translation="MNYLRASIIKKASTQVTKKVKDKTRFVSSRIGSAMLYDILQDID
                     NLDRDSRSLQEAKDLENITYLNPTYYYKKYGNNFSRAGNEIPLSERDRLIRSALYQVM
                     QVFDTVRYAANKVQGNKVANLLLGRIFKDTVVTNVVFYVSTQLYVYSEIFKSSKSSDM
                     KAIIEKVYKTREKWITGLSTKYMRIQMTKITQQNPYAVYFELWSYYKRPGRDKGYYNR
                     AMDFGVYTSGGTAMAGIRSKDDLPTLNELYGWFKRREAAGLYRPMVAVGWRSRRKYGL
                     SYKEPKNWLLKPYAAAYLTWIRMQRRFKKRGKLAPFGSFAKYNKRNTVYQTTIFTEIF
                     NKYMAYVLKRVKVEKAEIIEKTEDNFKFKVDRLIRSMNKNLKRTDRDLRSLNYLIRTK
                     NIRDISYAAIQSQYQVTDLKKLVRLAKKLEIKV"
     gene            complement(3882..4094)
                     /locus_tag="PLU55_04055"
     CDS             complement(3882..4094)
                     /locus_tag="PLU55_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87261.1"
                     /translation="MAEEIICQDLRENVLKGASYLKDLKRRNAYIELAKNVDYSTALA
                     DLLRELNELLESQYNQESLNPSNKEG"
     gene            complement(4154..>4570)
                     /locus_tag="PLU55_04060"
     CDS             complement(4154..>4570)
                     /locus_tag="PLU55_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87262.1"
                     /translation="LKKGEKMTVSLYKILAIKQALESLNLLAIPVIEDNDTQGVVSMI
                     IVGLADRPDDFNNLLKTISGEAKAWDDNLKEAEQVLLDFFALIPAELSVLINQLKSVS
                     STLENAVTQMMREEMLGIDNSVIPDQEQKQTTLETP"
     assembly_gap    4571..4670
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4671..5883)
                     /locus_tag="PLU55_04065"
     CDS             complement(<4671..5883)
                     /locus_tag="PLU55_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HPJ87263.1"
                     /translation="MVLDTIVEAQTKVIQALTEAGYQNVKDSLLCGITENEIYPFILV
                     DIDSSDTVNLASGLSTPVNHNLVISVYQKSSMGVKEARLSAAQEFDNIMQILNLSLTE
                     ERVEYLDTVMNGIKLCGVTAVLEVGLGESIEYHPLLGITSDPIGAILTIDNHPTNILT
                     PTVIRNARSGVYSALKEGYTYLPQTVDLSDRKDKTIMIPQSKGKLNAPNIYFDYGTGL
                     LHIDTEYTQCEIRYRLDGGDPHTDYQIWKDNIYIDTSQVRAYVIKEGYEDSDITEYNI
                     EVPFVKIVAEADNLDIISVIRIYPNVDITLTSNSENLLLSLDQTTWSNQVTALSGAYR
                     LIYLKAVETGTYYIYKSSGTIKYLGCEGTSSTLTYLFYRPFSLDYKNIICKCYLYYKT
                     VEGYTYFNQFRA"
     gene            complement(5888..6385)
                     /locus_tag="PLU55_04070"
     CDS             complement(5888..6385)
                     /locus_tag="PLU55_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87264.1"
                     /translation="MTHKQRVISNIESLSSLTESALAKISGNILTISARKMNELLGNS
                     AYTSYVELQNNTAIITEIEKDTWNYELATTSERQLKDLEMAEAYCALYYVALALKKID
                     MDTVMTQIETWGEGSIKPTDILRVVEFADKYLIEAKVIVTPYRASGEEVEGILTTGGV
                     GFIAI"
BASE COUNT         1779 a         1257 c          869 g         2389 t
ORIGIN      
        1 cataatggat ttcattcttt gtaactgttc attaagggtc atagctgcat tcttagcttc
       61 cataaaacca ttttgaatgg catacattat tttataggaa aacatagcag tccaaacgaa
      121 agacatacga gaaacaatct tatccagact aagatagtgt cctatagtag ccattatatt
      181 ttctttatga gctgcacctg tcttaattaa gagattacat ctgattaggg ttaaatcatt
      241 ctgttgtttc tccatttgta attgatgtaa ctgtaacatc tcttcgtttt gagactgcat
      301 atattgctgt ttttctaagg ctatttgttg tctttcttct tctgttacag cattggctaa
      361 catcctgtca taacgcaata aatcattatg catttggaga tatttggctc tttgtgcacc
      421 taaagcattt aatctggtct ctatatctct ctgtaaataa gcgttattgg ttaataactc
      481 ttctttctta tttaggacag cagttaagtt aattcttttg cctgcataaa cctgtctttg
      541 ttcatctaac tctactatct ttctttgagt atcatactct aaatcaatat ctttcttagc
      601 tatcttttct tcttcattta gctgtctctt agcctgtatt tgttctgctg tctgttgctt
      661 ctgtctttct cgttcttctt tatctctctg tctattttct gctttttgct gtctttctcg
      721 ttctttggct tctttatcat tttgcctttt tatctctgca tcttgtttta atgccaattt
      781 atcttctgca gctttcttct tagcttcatc tttatcttgt ttgatacgag cagcttcttc
      841 ttgacgagct tttctctttt ctgcttctgc tttcttattg tctgctttta tatcattgtt
      901 tactacttta atactatctg cattaacctt agtcagatta agttcctgac tgagtttctg
      961 taacttttca atatgagctt tatcatctgc ttcattaagt cccgttattt gtttcttaac
     1021 tgcagagatt tgagattcca aatcctttct tttcagtaac aagttattca aagcatctgc
     1081 tctctctact tctcccatca tagcataagc aggtttagca gctgttacag ctttggtata
     1141 ttcttcaggt gctaacttcc gagtagcttc tcctacagcc atagtcttag atttacccaa
     1201 ttcggaagtt attttcttag acatactttt gaaatcaagc atatattgag caacttgttc
     1261 tgcctcttca tctgttaaaa ataacttctc tttagcagga tatttttccg ttaacttacc
     1321 taaggcatct agctgagtta ataaaccttc cagattctta ctggtaaatg aataattttt
     1381 aattctattc ttgagttcat ctaagtattg aggagtaaaa ttggcaggaa cttcataaat
     1441 tttcttacct gcttgcatag cctgaaattc tgtttctgaa acaacattct gctttctgcc
     1501 taataattct ctgtatctga ttatatcttg ctgtttggct acactctcac ccgtgaactt
     1561 aattctttct tcaggcgtca tagaataaac aggacttttg gtaatgaagt ctattaagtc
     1621 cttttctctt tgttcaattg cttcagatac agctttagca gaagctttat tattgataaa
     1681 taaaacatcc ttaaataact tgcccaaatc atctgcgtta gctgttacat ctgctttagc
     1741 tttagcactt tggacattct tcattactcc accagcagct ctggcatctg cttcctgttt
     1801 gcttaaaact ttaactgctt ggctatactg agtctgtagt tgtctaagat tatttagttt
     1861 aacttcaatt ctttggacat taagagcatt atttaagatg tctccaggtt ctttagattg
     1921 tgataattca atttttgcca atcttagttg ttctttggtt ttagcaattt cttgtttggt
     1981 agaataaata actttatctc ttgtttcttg agacatattt ctaaagatac tatctataga
     2041 ttcacctatc attcctgtta tgcctttgaa cttggcaaaa ttcttatgta aagcatctcg
     2101 gaataaatca ctgaaattgg ttactacatt aacttgttct ctaatctcag gactaatgaa
     2161 ttgaccttct aaattgccac ctttaacagt ttctttggca cttataataa tacttcttaa
     2221 tcctgctaaa gtatgtttaa tctcctcatc cattgtttta ttcatttcca tagtcagctt
     2281 tctttgcata gcagtaagat tcttaagatt catttcagga gggataccaa cagcagtttg
     2341 agctacagat ttataatagc ctatagtttg aggttgttta tttattgcat ctcttagttc
     2401 ttttacttga ttgagttcat ctaataaatt gttaacttgc ttaatggaat tggctatacc
     2461 tgttcctgta gtaacatcaa aattgaattt attactttgt aagctactta aaatagaagc
     2521 tacacctgta acttcggaaa gtttatcaaa atcaatagat ttgatagcac tgccaatacc
     2581 tctgatggca gaagctaatt cattctgtcc tttaactttc cagacaatat ttacttcatt
     2641 agtagttttt gccattttat ccttcttata ccttaatctc cagcttttta gctaatctga
     2701 ctaacttctt caggtctgtt acctgatact gactttgaat agcagcataa cttatatctc
     2761 tgatattctt agttctaatc agataattaa gagacctgag gtctcggtct gttctcttca
     2821 gattcttatt catagatctg attaacctgt caaccttaaa cttgaagtta tcttctgttt
     2881 tttctataat ctcagctttc tcaactttta ctctttttag gacataagcc atatatttat
     2941 tgaatatctc agtaaagatg gtagtctgat agacagtatt gcgtttattg tatttggcaa
     3001 aactgccaaa aggtgctaac ttgcctctct ttttaaatct tctctgcatt cttatccaag
     3061 tcaaatatgc agctgcataa ggtttcaata accagttctt aggctcttta taacttaatc
     3121 cgtatttgcg tcttgatctc caacctactg ctaccatagg tctatacaaa cctgctgctt
     3181 ctcttctctt aaaccaacca tataactcat taagagtagg taaatcatct ttagatctaa
     3241 tacctgccat tgcagttcca ccactggtat agacaccaaa atccattgct ctattataat
     3301 aacctttatc tctaccaggt ctcttataat aagaccataa ctcaaaatag acagcataag
     3361 gattttgttg agttatttta gtcatttgta tccgcatata tttggtagat aagcctgtta
     3421 tccatttctc tctggtttta tagacctttt caataattgc cttcatatct gagcttttgg
     3481 atgatttgaa gatttcgcta tagacatata actgagtaga aacataaaaa accacattgg
     3541 taactacagt atctttgaat attctgccta acaagagatt ggctacttta ttgccttgca
     3601 ccttattagc tgcgtatcta accgtatcaa agacctgcat aacttgatat aaagcacttc
     3661 taatcaatct gtctcgttct gataagggaa tttcattacc tgctctactg aagttgttac
     3721 catatttctt ataataataa gtaggattga gataagtgat attctccaaa tctttggctt
     3781 cttgcaaaga tctgctatct ctatccaggt tatcaatatc ttgtaagata tcataaagca
     3841 tagcagaacc tatccgagaa gagacaaacc tcgtcttatc cttaaccttc tttgttactt
     3901 gggttgaggc tttcttgatt atactggctc tcaagtaatt catttaattc tctcaataaa
     3961 tctgctaaag cagtagaata atctacattc ttagctaact ctatataagc atttcttctc
     4021 ttcaaatcct tcaaataaga agcacctttc agaacattct ctctcaaatc ctgacagata
     4081 atctcttctg ccaagataag agcttctcct acatctataa gattacagtc tataccttta
     4141 gcacataata agattaaggt gtctctaaag tggtctgctt ctgttcttgg tctgggataa
     4201 ccgaattgtc tatccccaac atctcttctc tcatcatctg tgtaaccgca ttctccagcg
     4261 tggaacttac gctcttcaac tgattgatta aaacgcttaa ttccgctgga ataagagcga
     4321 aaaaatctaa gagaacctgc tctgcttctt ttaagttatc atcccaggct tttgcttctc
     4381 ctgagatagt cttcaacaga ttattaaaat catcaggtct atctgccaga cctacaataa
     4441 tcatacttac tactccttgt gtatcgttat cttcaataac gggaatagcc aatagattaa
     4501 gagattccaa agcctgctta atggctaaga ttttataaag cgatactgtc attttttctc
     4561 ctttttttaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cagctctaaa
     4681 ttgattaaaa taagtatatc cttctactgt cttataatat aagtagcatt tacagataat
     4741 atttttataa tctaaactaa atggtcgata aaaaagatat gtaagtgtgg aactagtacc
     4801 ttcacatccc aaatatttaa tagttccact gcttttgtat atataataag ttcctgtctc
     4861 aacagctttt agataaatta gtctataggc tccagataaa gcagttactt gatttgacca
     4921 agtagtttgg tctaagctta ataataaatt ctctgagtta gaggttaaag ttatatcaac
     4981 attgggataa attcttatta cactaattat atccagatta tctgcttctg ctacaatttt
     5041 aacaaaagga acttctatat tgtattctgt tatatcacta tcctcatagc cttctttaat
     5101 aacataagct cttacttgag aagtatctat atagatatta tctttccaga tctgatagtc
     5161 ggtatgagga tctcctccat ccaatctata tcttatttcg cattgagtat attctgtatc
     5221 tatatgcaat aaacctgtcc cataatcaaa atatatattg ggtgcattca atttgccttt
     5281 actttgaggt atcataatag tcttatcttt tctgtctgac aaatctacgg tttgaggtag
     5341 gtatgtataa ccctctttta acgcagaata aaccccacta cgagcatttc taatcacggt
     5401 tggagtaagt atattagttg gatgattatc tatcgttaaa atcgctccta ttgggtctga
     5461 ggttattccc aataaaggat gatattctat gctttcacct aagcctactt ccagaactgc
     5521 agtcacacca cataacttaa tcccgttcat aaccgtatcc aaatattcta ctctctcttc
     5581 tgtcaaactt aaattaagga tttgcattat attatcaaac tcttgagctg cagacagtct
     5641 tgcttcctta actcccatac tgctcttttg atagacagaa ataaccaaat tatgattaac
     5701 aggagtagat aaaccacttg ctaaattaac tgtatctgac gaatctatat ctaccaaaat
     5761 aaatggataa atctcattct cggttatacc acataataat gagtctttaa cattttgata
     5821 acctgcctct gttaaagctt gaataacctt agtctgtgcc tctacaatag tatccaatac
     5881 cataagctta gatggctata aaaccaactc ctcctgtagt taaaatacct tctacctctt
     5941 ctccacttgc tctataaggt gttacaataa ccttagcttc tattaaatat ttatctgcaa
     6001 actctactac tctcaaaata tctgtaggct taatagaacc ttctccccaa gtttctatct
     6061 gagtcattac tgtatccata tctatcttct taagagctaa agctacataa taaagagcac
     6121 aataagcttc tgccatttcc aaatctttga gttgtctttc ggatgtagta gctaactcat
     6181 aattccaggt atctttctca atttctgtaa taatagcagt attattttgt aactctacat
     6241 aactggtata agcagaatta cccaataact cattcatctt tctggcactt atagtaagga
     6301 tattgccact tatcttagct aaagcagact cagtgagact gcttaaactc tctatattgg
     6361 agataactcg ttgtttatgc gtcatttatg cctc
//
LOCUS       DAOPCH010000048         6369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_10744_length_6369_cov_4.306303, whole genome shotgun sequence.
ACCESSION   DAOPCH010000048 DAOPCH010000000
VERSION     DAOPCH010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10744_length_6369_cov_4.306303"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLU55_04075"
     CDS             <1..334
                     /locus_tag="PLU55_04075"
                     /inference="COORDINATES: protein motif:HMM:NF003239.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPJ87265.1"
                     /translation="LESCRQYVNKVLDKNIPGQYFFRVVPFPHHIQRENKMLTGAGAD
                     RMQTGMQLSFGKSVGKSAILKPNAEIFLIAVSTPKALIVARQAFKEIKSKLPCKTRVV
                     YELVSDSV"
     gene            409..942
                     /locus_tag="PLU55_04080"
     CDS             409..942
                     /locus_tag="PLU55_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87266.1"
                     /translation="MTDEKSKEKSKEELRAEMAMKNLKGSLWNYAAPKIVTHARYGEI
                     SDSIKYFYSNLIAKAPDQNIYNQLFLPQLMYEGGAITSPYLQNTSYAILQDDLLNIKV
                     EDALGLIGYKGTLNKELSGKYVKDTGSIAGLILDRVFRTKGDMFAKDILDTRIKEGPK
                     DLEAIIGEPENKEQGKQ"
     gene            1000..1491
                     /locus_tag="PLU55_04085"
     CDS             1000..1491
                     /locus_tag="PLU55_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87267.1"
                     /translation="MAKKTHTKKNVIKKFTYSLTPVEEIAVRNELPVKEVREAYRKCR
                     MAVLKETIQDIRKGKANINYFNLMDRVFSLTEEYLDAKRLKEIHEQEKLYCLNDFSVE
                     NGLTYSNVKNIYSRFNSRLFSNPDLNNLERVIDYNPELSQKAFDLTKKYFSIQAHGKL
                     NLR"
     gene            complement(1495..2364)
                     /locus_tag="PLU55_04090"
     CDS             complement(1495..2364)
                     /locus_tag="PLU55_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPJ87268.1"
                     /translation="MVKKKAKTKKVKKESKILEPKKEKSKVEIPTLKLKTEDEIAMDF
                     AIKVYKKFNKIVKSIVLFGSTAKQNVTPGSDIDIMIIIDDASVKWDQKLVIWYREELE
                     KIVQQSPYKKELHINTMKLTTWWEDMIRGDPTVMNIIRYGEAMIDAGGFFNPLKRLLL
                     EGKIKATPEAIYSCLQRAPLHYARSKEAELNAIEGLYWAMVDSSHAALIAGNISPPSP
                     EHIPGELKMNFVNSGKLKMKYVDWFRELNMMHKKIAHGEITDLKGVEIDAWQARTEDF
                     IRTMAQLVNKLIK"
     gene            complement(2389..3237)
                     /locus_tag="PLU55_04095"
     CDS             complement(2389..3237)
                     /locus_tag="PLU55_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87269.1"
                     /translation="MPTKKEIKKNQKAIEGLIKEGIEKMIEANPRFANYKGYFLRHAD
                     VKGVENMFNEIYETSREMGYNSQEARNKAISETRNYISSGSMLDERGKKALLEQGSYL
                     ILDEKSKKLIKREGKLERSLLQKITRPFKKTEGTDYLDRTTETFNELLYLIKEGGYNV
                     PEVEKPLEKLHYLGFASPAIALLAESKLIDKKKERYLLKNLYETHKQYTKEVVGAIEK
                     YVVPQKIAAAVIGFIGTFFLIFNLNMTGAVIGGDSNVTMGILGVFMIFFALMLYLRPL
                     KKSFKK"
     gene            complement(3272..5080)
                     /locus_tag="PLU55_04100"
     CDS             complement(3272..5080)
                     /locus_tag="PLU55_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPJ87270.1"
                     /translation="MAKKGGYIIENVYQGGYSTLDPNKAYSSSFTGYRANVGSLGITT
                     NPGTINQIKEVSDKLASGLKNVEIEFIEPRVMDAIPKQQLTEIRQLSKLVGGDVSVHG
                     PVIDSTGMGEQGFSELNRELAERKITEALLRSHELKPDGNITVNFHSAQGIPSSTWKT
                     LGDVEGKKPREFKRMVAVERETGKMIHLDTEKKYYPGEDLSKGETYTPERNLESLNAT
                     SWDNQLTQLFFNKERADQILGENGAMIQDVLGSIEEIKKKGLNPYEVLSKPQQNALAR
                     YYDAQRYLEEINRQARSIFSKGYEFGNDKQKRELAKISEQYKEDIQKAGIDPLAQSMA
                     IRSLLNELQNPKLAPEMFVPIEEFATEQSAKTFGNAAFNAFDKFKDPNKTPITLIENP
                     PAGFGLSTGEDLRNLVVESRKKFVEKAVKEKNMSEKEAEKIAEKLIGATWDVGHINML
                     RKEGFKEQDIIRETEKIAPYVKHIHLSDNFGFEHTELPMGMGNVPMKEIMEKLGEKGF
                     EAKKIIEAGDWWTGMKTSPVKEALEGLGSPMYMTGPGPYWNQSIGLQQNYLSGYGMML
                     PSNNYQIFGAGFSQLPQDLGGDMKSSGGRFSQRGME"
     gene            complement(5144..5758)
                     /locus_tag="PLU55_04105"
     CDS             complement(5144..5758)
                     /locus_tag="PLU55_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87271.1"
                     /translation="MEKKPKNKSSLKKLEQPKKEKSKIKKLNLTLSQEEVNSLEKMIK
                     EDSGKTNSPKVQELLKNTNINFSPSLKKINAPEKSQIVLERNIITGNIDILNLGETKK
                     RSSEEINYIPSTTEEEKKYTPRYNERIANFTQRREIEKAPPENFFRFREIKAGDLYKE
                     KIQEPEKFEKYSSPKTTMERFDILKKPKKDPFNRKEIKYTPSEY"
     gene            complement(5814..>6369)
                     /locus_tag="PLU55_04110"
     CDS             complement(5814..>6369)
                     /locus_tag="PLU55_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87272.1"
                     /translation="IPKDFAKESIYKKMRKYYSCILVEFTFRGIPQRISQQSHFTFGG
                     KADITFKAYALNEDELNKLNRELEKSDLEDVLKLIEGATDESLNQLQEEINSFLNEKE
                     EKEKEKEQKDTSNPFLALFGFYNTKEEKKEAPKKDNKEDKPITKDNWVEKTHLRPLAE
                     EGATSTAMALFDIYKKSHGMASYT"
BASE COUNT         1803 a         1015 c          874 g         2677 t
ORIGIN      
        1 tcttgaatct tgcaggcaat atgtaaataa agttttagat aagaatattc caggacaata
       61 ctttttcagg gttgttcctt ttccacatca tattcaaaga gaaaacaaaa tgcttactgg
      121 tgcaggagct gatagaatgc agacaggaat gcaattatct tttggtaaaa gtgtaggaaa
      181 atcagctata ttaaaaccaa atgctgaaat attccttatt gctgtttcaa caccaaaagc
      241 acttatagta gcaagacagg cattcaagga aataaaatct aaacttcctt gtaaaactcg
      301 agtagtttat gagttagttt ctgattctgt ttaaaataag gatttgtcac ttttgtttaa
      361 tggtaacatt aacgaaagat ttataaactt caagtttcta taatttacat gacagatgaa
      421 aaatcaaaag aaaaatcaaa ggaagaatta agagcagaaa tggctatgaa aaaccttaaa
      481 ggttcactgt ggaattatgc tgctccaaag atagttacac atgcaagata tggtgaaatt
      541 tcagattcaa ttaaatattt ctattcaaat ctaatagcta aagctcctga tcagaatatt
      601 tataatcaat tatttttacc tcaacttatg tatgagggtg gagctataac aagcccttat
      661 cttcaaaata cttcttatgc aatattacaa gatgatttat tgaatattaa ggttgaagat
      721 gctttaggac ttattggata taaaggaaca cttaataaag aattatctgg aaagtatgtt
      781 aaagatacag gaagtattgc aggattaatc ttagatagag ttttcagaac taaaggagat
      841 atgttcgcta aagatattct tgatactaga ataaaagaag gtccaaaaga tttagaagca
      901 ataattggtg aacctgaaaa taaagaacaa ggcaagcaat aatcttcata gatagattaa
      961 taaactcgct ttttgattgt tttttactta tttattaaaa tggcaaaaaa aacacataca
     1021 aagaaaaatg taattaaaaa attcacttat agtttgactc ctgttgaaga gattgctgta
     1081 agaaatgagt tgcctgtaaa ggaagtgaga gaagcttata gaaaatgtag gatggctgtt
     1141 cttaaagaaa caatccaaga tataagaaaa ggtaaagcaa atattaatta ttttaatctt
     1201 atggacagag ttttttcttt aactgaggaa tatttggatg ctaaaagact taaagaaata
     1261 catgaacagg aaaaacttta ttgtcttaat gatttttctg ttgagaacgg attaacttat
     1321 tcaaatgtta aaaatattta ttctagattt aattcaagat tattttctaa tccagattta
     1381 aataatcttg aaagagttat tgactataat cctgaattaa gtcaaaaagc atttgattta
     1441 actaagaaat atttttctat tcaagcacat ggtaagttga atctaagata aattttattt
     1501 tattagtttg tttactagtt gagccattgt tcttatgaaa tcttctgttc ttgcctgcca
     1561 agcatctatt tcaacccctt ttaggtctgt tatttcacca tgagctattt ttttatgcat
     1621 catatttagc tctctaaacc aatccacata tttcattttt aattttcctg aatttacaaa
     1681 attcattttt aattctcctg gtatgtgttc tggagagggt ggagaaatat ttcctgcaat
     1741 gagtgcagca tgagaagaat caaccattgc ccaatacaat ccttctattg catttaattc
     1801 agcttctttg cttcttgcat aatgtaatgg agctctttgt aaacaagaat aaattgcttc
     1861 tggtgttgcc tttatttttc cttctaatag caatcgtttt aatggattga aaaatcctcc
     1921 tgcatctatc atcgcttcac catatcttat aatattcata actgtagggt ctcctcttat
     1981 catatcttcc caccaagttg tcaatttcat tgtgtttatg tggagttctt ttttatacgg
     2041 gctttgttga actatttttt caagttcttc tctgtaccaa ataactaatt tttggtccca
     2101 ttttactgaa gcatcatcta ttattatcat tatatctata tcagaaccag gagtaacatt
     2161 ttgttttgct gttgacccaa ataaaactat agattttaca attttattga attttttata
     2221 tactttaatt gcaaaatcca ttgcaatttc atcttctgtt tttaatttta atgtaggaat
     2281 ttctacttta gatttttctt ttttaggttc taaaattttg ctttcttttt tcaccttttt
     2341 tgtttttgct ttctttttca ccatctttta cttttttata aagatatttt actttttaaa
     2401 gctttttttt aaaggtctca aataaagcat gagtgcaaaa aatatcataa acactcccaa
     2461 gattcccatt gttacgtttg agtcccctcc tataactgct cctgtcatat tcaaattgaa
     2521 tattaaaaag aatgttccta taaatcctat gaccgctgca gcaatttttt gtggaacaac
     2581 atatttttct attgcaccaa ccacttcttt tgtgtattgt ttgtgagttt catagagatt
     2641 ttttaataag tatctttctt ttttcttgtc tatgagttta gattctgcaa gcaaagctat
     2701 tgcaggagag gcaaaaccaa gataatgaag tttctcgagt ggtttttcta cttctgggac
     2761 attatagcca ccttctttta ttaggtaaag caattcatta aaagtttctg ttgttctatc
     2821 aagataatct gttccttctg tttttttgaa tggtctagta attttttgaa ggagacttct
     2881 ttcaagtttt ccttctcttt ttattagttt ttttgacttt tcgtcaagaa tcaagtaact
     2941 tccttgttct aatagtgctt tttttcctct ttcatctaac atacttcctg aacttatgta
     3001 gtttcttgtt tctgatattg ctttattcct agcttcttga gaattgtaac ccatttctct
     3061 gcttgtttca tatatctcat tgaacatatt ttccacacct ttaacatcag catgtcttaa
     3121 gaaatatcct ttgtagtttg cgaatcttgg attcgcttct atcatctttt ctatcccctc
     3181 tttaattaat ccttctattg ctttctgatt ttttttaatt tccttttttg ttggcatgtt
     3241 tattttaagt ttttttgatt aataaattta tttactccat tcctctttga ctgaatctcc
     3301 ctccagagct tttcatgtct ccccctaaat cttgggggag ctgagaaaat cctgctccaa
     3361 atatttgata attatttgaa ggaagcatca ttccatatcc agagagataa ttttgttgta
     3421 atccaataga ttggttccaa tatggtcctg gtcctgtcat atacatcgga gaacctaatc
     3481 cctcaagagc ttcttttaca ggagaagttt tcattcctgt ccaccaatct cctgcttcaa
     3541 ttattttctt agcttcaaat cctttttcac ctaatttttc cattatctct ttcataggca
     3601 catttcccat acccataggt aattcagtgt gttcaaatcc aaaattatca gatagatgaa
     3661 tatgttttac ataaggggct attttttctg tttctcttat tatatcttgt tctttgaacc
     3721 cttcttttct taacatattt atatgtccta catcccatgt tgcacctatt aatttttcag
     3781 ctattttttc tgcttctttc tcactcatat ttttttcttt tactgctttt tcaacaaatt
     3841 tttttcttga ttcaacaact aaatttctta aatcttctcc agtagataat ccaaatcctg
     3901 caggaggatt ttctataagt gttataggtg ttttgtttgg gtctttgaat ttgtcaaatg
     3961 cattaaatgc agcatttcca aatgtttttg ctgattgttc tgttgcaaat tcctctatag
     4021 gtacaaacat ttcaggagca agttttggat tttgtaattc atttaataaa cttcttatag
     4081 ccattgattg agctaatggg tctattcctg ctttttgtat gtcttcttta tattgttcag
     4141 aaatttttgc taattctcgt ttttgtttat cattaccgaa ttcatatcct ttactaaata
     4201 ttgatctagc ttgtctattt atttcctcaa ggtacctttg agcatcataa tatcttgcta
     4261 atgcgttttg ttgaggttta cttaatactt catatgggtt taatcccttt ttctttattt
     4321 cctctataga tcctaataca tcctgaatca tagccccatt ttctcctaat atctggtctg
     4381 ctctttcttt gttgaaaaat aattgtgtta attgattgtc ccagctagtt gcatttagtg
     4441 attctaaatt cctttctgga gtatatgttt cgcctttaga taaatcttct ccagggtaat
     4501 attttttttc tgtatctaaa tgaatcattt taccagtttc tctttcaact gcaaccattc
     4561 ttttgaattc tcttggtttt tttccttcaa catctcctag tgttttccaa gttgaacttg
     4621 gaattccttg tgcagaatga aaattaacag ttatgtttcc atctggtttt agttcatgac
     4681 ttcttaaaag agcttcagtt atttttcttt cagctaattc tctgtttagt tcactaaatc
     4741 cttgctcacc cattccagtg ctgtctataa caggaccatg aactgaaaca tctcctccaa
     4801 ctaatttact taattgtctt atttctgtaa gttgttgctt tggaatagca tccattactc
     4861 ttggttctat aaattcaatt tctacatttt ttaaaccaga ggcaagtttg tctgatactt
     4921 cttttatttg atttattgtt cctggatttg tagttatacc tagtgaacct acatttgctc
     4981 tataccctgt aaatgaacta gaataagctt tgtttgggtc tagagtgcta tatcctcctt
     5041 gatatacatt ttcaattatg tatcctcctt tttttgccat attatatact gtactatata
     5101 atttattaag ttaatggttt taattttaaa tataatatgt tattcaatat tcactcggag
     5161 tgtacttaat ttcttttcta ttaaaagggt ctttttttgg tttttttaat atatcaaatc
     5221 tttccattgt tgtttttgga gaagaatatt tttcaaattt ttcaggttct tgtatttttt
     5281 ctttatataa atctcctgcc tttatttctc tgaatctaaa gaaattttca ggaggtgctt
     5341 tttcaatttc tcttctttga gtaaaattag caattctttc attataacgg ggtgtgtatt
     5401 ttttttcttc ttcagttgtg cttggaatgt aattaatttc ttctgaactt cttttttttg
     5461 tttctcctaa attgagtata tctatatttc ctgttattat atttctttca aggactattt
     5521 ggcttttttc tggagcattt atctttttta atgaaggaga aaaatttata tttgtatttt
     5581 ttaaaagttc ttgcactttt ggagagtttg tttttccact gtcttcttta atcatctttt
     5641 caagagaatt tacttcttct tgactgagtg ttaaatttaa tttttttatt ttgctttttt
     5701 ctttttttgg ttgttctagc ttttttagag atgatttgtt ttttggcttt ttttccatta
     5761 tttaattagg attaagaaat atataatagt ttttatttgt aaatttgaag tttttaagtg
     5821 taacttgcca ttccatgtga tttcttgtaa atatcaaaaa gagccattgc agtacttgtt
     5881 gcaccctctt ctgccagagg tcttaagtga gttttttcaa cccaattatc ttttgttatt
     5941 ggtttatctt ctttattatc ttttttagga gcttcttttt tttcttcttt tgtattataa
     6001 aatccaaaca gagcaaggaa aggattagaa gtgtcttttt gttctttctc tttttctttc
     6061 tcttcttttt catttaaaaa agagtttatt tcttcttgca attgatttaa gctttcatct
     6121 gttgctcctt ctattaattt caacacatct tctaaatcag atttttcaag ttctctgttt
     6181 agtttgttta attcatcttc atttaaagca tatgctttga aagttatatc tgcttttcct
     6241 ccaaatgtaa aatgagattg ctgagaaatt ctttgaggta ttcctctgaa tgtgaattca
     6301 actaatatac aactataata ttttctcatc tttttgtaga ttgattcttt tgcaaaatct
     6361 tttggaatt
//
LOCUS       DAOPCH010000049         6353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_10782_length_6353_cov_3.952366, whole genome shotgun sequence.
ACCESSION   DAOPCH010000049 DAOPCH010000000
VERSION     DAOPCH010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10782_length_6353_cov_3.952366"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLU55_04115"
     CDS             complement(<1..466)
                     /locus_tag="PLU55_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPJ87273.1"
                     /translation="MTPNIDPKAEENLKKGIFPREVSEEMKKSYLDYAMSVIVSRAIP
                     SIEDGLKPVQRRILYSMHQMGLKPNTATKKSARIVGDVIGKYHPHGDSAAYDALVRMA
                     QDFSLRYPLVYGQGNFGSIDGDPPAAYRYTEAKLMPISQELLQDLEKDTVNHL"
     gene            complement(468..788)
                     /locus_tag="PLU55_04120"
     CDS             complement(468..788)
                     /locus_tag="PLU55_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87274.1"
                     /translation="MAKKGKLTTPAWILEGYDSPEEYAKAKGKSAPDKKQGKTFKIKI
                     CPKCKSDDVVITLSNMDFEEESSTGKQWECKKCKWTGTDIKEKQLTEDELMEFLDKKG
                     EEVA"
     gene            complement(790..1179)
                     /locus_tag="PLU55_04125"
     CDS             complement(790..1179)
                     /locus_tag="PLU55_04125"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPJ87275.1"
                     /translation="MEKTIVVTIIENNKKELLLQKKSLDYPVYPGVWCFVGGEAESED
                     FHKEMLREIKEELGIKMNVKFMFTEKTISKRGKSTFYVFLSKLNDLSKIKIKEGVGIA
                     LFGKKELNQLRIDPETKKILIKYFKEF"
     gene            complement(1181..3070)
                     /gene="gyrB"
                     /locus_tag="PLU55_04130"
     CDS             complement(1181..3070)
                     /gene="gyrB"
                     /locus_tag="PLU55_04130"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPJ87276.1"
                     /translation="MAEKKDYTANDIKVLEGLEGIRKRPAMYIGSTGKEGLHHLVYEV
                     VDNSVDEALAGFCDRIKVTLNKDGSVTIDDDGRGIPVDIHPDYKVPAAQIALTKLHAG
                     GKFDKKSYAISGGLHGVGVSCVNALSKKLILEIKRDGKIHQQEYSRGNPTTKMKVVGD
                     TNETGTKITFYPDEQIFSTTTFDFKVLESRFREVTFLNAGLKIELRDEIKDTSQEFFS
                     SGGLVEFVRWINKSKEILHKPIYFKRQMTNGAVEIAIQYNSTYQENIFGFVNTINTVE
                     GGTHISGFKTALTRVINDYAKKKDLLKNDSFSGDDVREGLTAIVSIKISEPQFEGQTK
                     TKLGNSEVKGIVDSIVTSALTEFFEENPQVAKKIADKSLQAAKARLAAKKAKDLVRRK
                     NAFSLGGLPGKLADCSDKKSEETELYLVEGESAGGSAKMARNKEIQAILPLRGKILNV
                     EKANPSKALSSEEITNMITVIGTGIGEQFDPAKIRYGKIIMMTDADVDGEHIKTLLLT
                     FFFRFMPQLIEHGHIYAAMPPLYRVRKGQKDIYVYSDEELKKVTAELGVNEGGVTRFK
                     GLGEMNSTQLWDTTMNPKTRKIKKIYIKDAIEADRVFTMLMGDDVQARRDFIQENAHE
                     AELDV"
     gene            complement(3242..3808)
                     /locus_tag="PLU55_04135"
     CDS             complement(3242..3808)
                     /locus_tag="PLU55_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPJ87277.1"
                     /translation="MYKILVSDIMTREPLVISPNANLLECAKKMVKKKTGSLLLVEKK
                     KLIGLISTQDILWALVKKSQKDLSSIQAKDISPKKIAVIKPSATLEEALKKMKKSRFE
                     RLPVIDNNELVGLITTKDILSFNPEIYPELQEFAEIREEARKLKLVKKAKEKKMSGFR
                     EGICEECGNYGRLERHKGMLICVSCKEA"
     gene            3964..4206
                     /locus_tag="PLU55_04140"
     CDS             3964..4206
                     /locus_tag="PLU55_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87278.1"
                     /translation="MDRQYRTKINLEGEYAYEDVSLFLEKISLECDKYKHGGVYFLDK
                     DRYQRIRVDSKNKTVELTDKVEEWIFNSINELSIKK"
     gene            4208..5836
                     /gene="thsA"
                     /locus_tag="PLU55_04145"
     CDS             4208..5836
                     /gene="thsA"
                     /locus_tag="PLU55_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HPJ87279.1"
                     /translation="MTNKPITLMPEDIQRIIGKDAQRNNIAAAKLVAEIVKTTLGPRG
                     MDKMLVSPLDDIIITNDGATILSEMQIEHPAAKMMVEIAKTQESEVGDGTTSAVMIAG
                     KLLENAEKLLDKKIHPIVIVKGYRIAAEKAQDILKELSMEVDHKDEFVLKQIAMTAMT
                     GKGAEDSKENLSDIVVKAVNQIEENGFVDLDNIKIEKARGEGIGDSELISGIVIDKGK
                     ITLDMPSYVKNARIALIDIPLELKSPELKTEVSISSPEQLQKFLLQEENSIKEIVNLV
                     RESTANVVFCQKGVDDFASFLLARNGIYVCRRVARSDMHKIAKATGAKIISNLNDLTQ
                     LELGSAESVENVKRGNDFYTYIQGCKNPKAVTILIHGGSDHVMDEIERAIDDGLGDVS
                     CVLKSGLVVPGGGAIEMEVSRRLKQFGQTISGRERLAVEEFANALEFIPLTLAENAGL
                     DPIDIITELRAKHDSGDKNAGLNLFENKVANVLEAKIIEPYNIKSQEIDSATEVANMI
                     LRIDDVIASKPDKKGKMSFTGERGREAEFMSGLE"
     gene            5883..>6353
                     /locus_tag="PLU55_04150"
     CDS             5883..>6353
                     /locus_tag="PLU55_04150"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPJ87280.1"
                     /translation="MYYHIILTEKCNSRCKYCYQKSMEEFDNGLKDKFEFDYSAPCES
                     TVKGVDVARFLKDGDCLIFYGGEPLVNFQKMKEIIDSVEKSKKKIKFYIQTNGKLLDE
                     IPLIYINKMSKMLVSIDGDMRRTDYNRGFGTYNKVLNNLIELREKGYKGEIVARM"
BASE COUNT         1981 a          946 c         1018 g         2408 t
ORIGIN      
        1 gtaaatggtt tacagtgtct ttttctaaat cttgtaaaag ttcttgagag attggcatta
       61 gttttgcttc agtataccta taagcagctg gagggtctcc atcaattgaa ccaaaatttc
      121 cttgaccata tacaagagga tatcttaagg agaaatcttg agccattctt actaatgcat
      181 catatgcagc actatctcca tgagggtggt acttaccaat aacatctcct actattcttg
      241 cagatttttt tgttgcagta tttggtttta aacccatttg gtgcattgag tataatattc
      301 ttctttgaac tggttttaat ccatcttcaa ttgaaggaat agctcttgat actatgacgc
      361 tcatagcata atcaagataa gattttttca tttcttcaga aacttctcta ggaaaaattc
      421 cttttttcag attttcttca gcttttggat caatgtttgg tgtcatttta tgcaacttct
      481 tctccttttt tatctaagaa ttccataagt tcatcttcag ttaattgttt ttcttttata
      541 tctgttccag tccatttaca ttttttacat tcccattgtt ttcctgtact gctttcttct
      601 tcaaagtcca tattgcttag tgttattaca acatcatcac ttttgcattt aggacagatt
      661 tttattttga atgtttttcc ttgtttttta tctggagctg attttccttt ggctttagca
      721 tattcttcag gtgaatcata cccttctaat atccatgcag gtgttgttag ttttcctttt
      781 tttgccattt taaaactcct tgaaatattt tattaatatt ttttttgttt caggatctat
      841 tctaagttga tttaattctt tttttccaaa caatgcaatt cctacacctt cctttatttt
      901 tattttagat aaatcattta atttacttaa gaatacataa aatgttgatt tgcctctttt
      961 agaaattgtt ttttcagtaa acataaattt tacattcatc ttaattccaa gttcctcttt
     1021 tatttctctt agcatttctt tgtgaaaatc ttctgattca gcttcccctc caacaaaaca
     1081 ccaaactcca ggataaacag ggtaatctaa acttttcttt tgtaaaagca attctttttt
     1141 gttgttctct atgattgtta ctacgattgt tttttccatt ttatacatct agttctgcct
     1201 catgtgcatt ttcttgaatg aaatctcttc ttgcttgtac atcatctccc ataagcattg
     1261 tgaatactct gtctgcttca attgcatctt taatatatat tttttttatc ttccttgttt
     1321 taggattcat tgttgtatcc cataattgtg ttgagttcat ttcccctaaa cccttgaaac
     1381 gtgtaacccc tccttcattt actcctaatt ctgctgttac ttttttgagt tcctcatctg
     1441 agtaaacata aatatctttt tgtccttttc ttactctata taatggaggc attgcagcgt
     1501 agatgtgtcc atgctcaatt aattggggca tgaatctgaa aaagaaagtt aaaagaagag
     1561 tttttatgtg ttctccatca acatctgcat ctgtcatcat tatgattttt ccatatctta
     1621 tttttgctgg gtcaaattgt tctcctattc cagttcctat aactgtaatc atatttgtta
     1681 tttcttcact tgaaagagct tttgatggat ttgctttttc aacattgagt attttacctc
     1741 taagaggaag aattgcttgt atttctttat ttcttgccat tttagcagag cctcctgcag
     1801 attctccttc aactaaatat aattcagttt cttctgattt tttatctgaa caatctgcaa
     1861 gctttccagg taatcctcct aatgagaatg cattttttct tctgaccaaa tctttggctt
     1921 tttttgcagc aagtcttgct ttagcagctt gaagagattt atctgctatt ttctttgcta
     1981 cttgtggatt ttcttcgaag aattctgtta gggcagaagt aactattgaa tcaacaattc
     2041 cttttacttc agaatttcct aattttgttt ttgtctgtcc ttcaaattgc ggttctgaaa
     2101 ttttaatact tactattgct gttaatcctt ctcttacatc atctcctgaa aatgaatcat
     2161 tcttaagtaa atcttttttc tttgcataat catttattac ccttgtaagg gcagtcttga
     2221 aaccagaaat atgagttcct ccttcaacag tatttattgt atttacaaat ccgaatatat
     2281 tttcctggta agttgaattg tattgtattg caatttcaac tgcaccatta gtcatttgtc
     2341 ttttaaaata aataggtttg tgtaatattt ctttggattt gtttatccac cttacaaatt
     2401 caactaatcc tccacttgag aaaaattctt gtgaagtgtc ttttatttca tctcttaatt
     2461 ctattttcaa tccagcattt aggaaagtta cttctctaaa tcttgattct aaaactttga
     2521 aatcaaaagt tgttgttgaa aatatttgtt catctggata aaaggttatt tttgttcctg
     2581 tttcatttgt atctcccaca accttcattt ttgttgttgg atttcctctg gaatattctt
     2641 gttgatgtat tttaccatca cgttttatct caaggattaa tttttttgaa agagcattaa
     2701 cacaggaaac tcctactcca tgtaatcctc ctgaaatagc ataagatttc ttatcaaatt
     2761 ttcctcctgc atggagtttt gttaaagcta tttgtgctgc aggaactttg taatctggat
     2821 gaatgtctac aggtatacct cttccatcat catcaatagt tacagaacca tctttgttta
     2881 gagttacctt tattctatca cagaatccag ctaaagcttc atctacagaa ttatcaacta
     2941 cctcataaac taagtgatgt aatccctctt ttcctgttga accaatgtac atagcaggtc
     3001 tttttctgat tccttctaat ccttctaata ccttgatatc attagcggta taatcttttt
     3061 tttctgccat cttataaaaa atctgtattt tctatattat ttctctttat tattccgtaa
     3121 aaaagaaaac cccttttata aatctttctg ctattttatc gtcgataaaa taaaaaaatg
     3181 agtaaaaatc agttaaaata tttaaattat ttttaattaa tttactatag aataaaattc
     3241 tttaagcttc cttacaagag acacaaatta gcattccttt atgtctttct aatcgcccat
     3301 aatttccaca ttcttcacat attccttccc taaaacccga catctttttt tcttttgctt
     3361 tttttacaag ctttaatttt cttgcttctt ctcttatttc agcaaattct tgtaattcag
     3421 gataaatttc aggattaaag cttaagatat cttttgttgt aattaatcca actaattcat
     3481 tgttgtctat gactgggagt ctttcaaatc tggatttttt cattttcttt aaagcttctt
     3541 caagtgtagc acttggtttt attacagcaa ttttttttgg agagatgtct tttgcttgta
     3601 ttgatgaaag gtctttttgg gactttttta caagtgccca gagaatatcc tgtgttgaaa
     3661 tcaaccctat aagcttcttt ttttcaacaa gaagaagact tcctgtcttt tttttgacca
     3721 tctttttagc acattctagt aaattagcat ttggactaat aacaaggggt tctcttgtca
     3781 ttatatcaga aaccagtatt ttgtacatat ttttacaggg ttttagcact tataaatatt
     3841 ttttaccact tcaaaaatat aagtcttgtt taaaaaatga taatattgaa tttcaataca
     3901 ataatgctta aataaatgat ttgagaacat tttttattag ggggaggaac atattaaact
     3961 gaaatggata gacaatatag aactaaaata aatcttgagg gagaatatgc ttatgaagat
     4021 gtaagtctat ttttagagaa gattagcttg gagtgtgata aatataaaca tggaggtgtt
     4081 tattttcttg ataaagatag ataccaaaga ataagggtag attcaaaaaa taaaacagtc
     4141 gaattaacag ataaggttga agaatggata tttaatagca taaatgaatt aagtataaaa
     4201 aagtgatatg acaaataaac caataacctt aatgcctgaa gatatacaga gaattattgg
     4261 taaagatgct caaaggaata acattgctgc agctaaactt gttgcagaga ttgttaaaac
     4321 gactttgggg ccaagaggca tggataagat gcttgtttct cctttagatg atataattat
     4381 aactaatgat ggagcaacta ttttaagtga gatgcaaatt gaacatcctg ctgcaaaaat
     4441 gatggttgag attgctaaaa ctcaagaatc tgaagttgga gatggaacaa cttctgctgt
     4501 gatgattgca ggaaaacttt tagaaaatgc agagaagctt ttagataaga aaatacatcc
     4561 tattgttatt gttaaaggat atagaattgc tgcagaaaaa gctcaggaca ttttgaaaga
     4621 attgtctatg gaagttgatc ataaagatga atttgttttg aaacagattg caatgactgc
     4681 aatgactggg aaaggagcag aagattctaa ggaaaatctt tcagatattg ttgttaaggc
     4741 agttaatcag attgaggaaa atggttttgt ggatttagat aatataaaaa tagagaaagc
     4801 aagaggagaa ggtattggag atagtgaatt aatatctggg attgttattg ataaaggaaa
     4861 aataaccttg gatatgcctt cttatgttaa aaacgcaaga attgctttaa ttgatattcc
     4921 tttggaactt aaatctcctg aattaaaaac agaggtttca atttcctctc ctgaacaatt
     4981 acaaaaattt ttgcttcaag aggaaaattc aattaaagag attgttaatt tggtgagaga
     5041 atcaactgct aatgttgtat tttgtcagaa aggtgttgat gattttgcaa gttttctttt
     5101 agctagaaat ggaatttatg tttgtagaag agttgcaaga agtgatatgc ataagatagc
     5161 aaaagctaca ggtgctaaga taataagtaa cttaaatgat ttaacacaac ttgaattagg
     5221 ttcagcagag agtgttgaaa atgttaagag gggaaatgat ttttatactt acatccaagg
     5281 gtgcaaaaat cctaaggctg ttactatttt aattcacggt ggaagcgacc atgttatgga
     5341 tgaaattgag agagcaatag atgacgggtt aggtgatgtt tcttgtgttt taaaatctgg
     5401 attagttgtt cctggtgggg gggctattga gatggaggtt tccagaagat taaaacaatt
     5461 tggacagact atttcaggaa gagaaagatt agctgtagaa gaatttgcaa atgctttgga
     5521 atttatacct cttactcttg ctgaaaatgc tggtttagat ccaatagaca ttatcactga
     5581 attaagggct aaacatgatt caggagataa gaatgctgga ctgaatttat ttgagaataa
     5641 agttgcaaat gttcttgaag ctaaaataat agaaccttat aatataaaga gtcaggaaat
     5701 tgattctgca acagaagttg caaatatgat tttaaggata gatgatgtta ttgcttctaa
     5761 gccagataag aaaggaaaaa tgagctttac aggtgagaga ggaagagaag cagaatttat
     5821 gagtggatta gagtagaatt cttctcaata atacttaaaa accagtattt ctagtatttt
     5881 ttatgtatta tcatattata cttacagaaa aatgcaattc cagatgcaaa tattgttatc
     5941 aaaagtcaat ggaagaattt gataatggac ttaaagataa atttgaattt gattatagtg
     6001 ctccgtgcga atcaacagtt aaaggagttg atgttgcacg attcttgaaa gatggagatt
     6061 gtttgatatt ttatggtgga gaacctttag ttaattttca gaaaatgaag gaaataatag
     6121 attctgttga gaaatctaaa aagaaaataa aattctatat acaaactaat ggaaaattgc
     6181 ttgatgaaat acccttgatt tacatcaaca aaatgtctaa gatgcttgtt tcaatagatg
     6241 gagatatgag aaggacagat tataatagag gttttggaac ttacaataag gtccttaata
     6301 atttaattga attaagagaa aaaggatata agggagagat tgttgcaagg atg
//
LOCUS       DAOPCH010000050         6295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_10902_length_6295_cov_4.473237, whole genome shotgun sequence.
ACCESSION   DAOPCH010000050 DAOPCH010000000
VERSION     DAOPCH010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10902_length_6295_cov_4.473237"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="PLU55_04155"
     CDS             <1..799
                     /locus_tag="PLU55_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPJ87281.1"
                     /translation="DFSGTACSGKVLVLIQSIINKYPVEFASALGALLSTVVIFLLVV
                     WAQSLKVEIPLSFGRIRGYGIKWPLNFFYASVIPVILTAALVANIQLFGGLLENWVGH
                     PTFLGHFSNGQAVSGFAFWLGHTNLLELIIRGGFLPRYLIQGITHIIFYVGLATLFSV
                     FWVKTSGMGAKEQAQKISASGLQISGFRQDVRVLESVLDRYIMPLTVMGGAAIGLLTA
                     TTDLLGALSSGTAILLVIMIMYQFYQKIAEQHMTDMNPAMRKFISNN"
     gene            862..1428
                     /locus_tag="PLU55_04160"
     CDS             862..1428
                     /locus_tag="PLU55_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HPJ87282.1"
                     /translation="MEETNLKKDKNAKEGSFAPLIIFMILAMVLAGLWDKIPIIKNSI
                     HYILDPSAGVLLNWKLNLGMLIIVLVITTITTIVQKYATDQKTLKEMRKEQKEMQKQM
                     NEFKNNPDKLMELQKKQFAMMPKQMKLSMRAIIYTGIPFILFFRWFNDYFIAAGSPRF
                     WLGLSWFWFYLIFAMIFGSFLRKWFDVA"
     gene            1476..1928
                     /gene="pfdA"
                     /locus_tag="PLU55_04165"
     CDS             1476..1928
                     /gene="pfdA"
                     /locus_tag="PLU55_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HPJ87283.1"
                     /translation="MAKENQESQQELVMQFQIFEQQIRMIQEQLQAVEQAIVELGSLN
                     FGLDELVGKKDEEILSPIGRGIYAKSKLISEELLVDIGNKNFVKKSIPETKKIIQNQI
                     KKLEKAKEELNKELEKINTELTEVFMKHQSSHKHDQCDCGEEECDCKK"
     gene            complement(1929..2744)
                     /locus_tag="PLU55_04170"
     CDS             complement(1929..2744)
                     /locus_tag="PLU55_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87284.1"
                     /translation="MNKKKRFNEIVRKIKNVEIQGAKNVARAALEAYFLLPSKDSKEK
                     LMSSRPTEPMMFYVLNLAEKGVSKKEILNHFEFSQKEINKHVLKLINNRDVIFTHCHS
                     TNVVNSLIYSKKKGKKFKVYNTETRPLFQGRKTAVELMKNGIDVTMFVDSAAAFAIKK
                     DDKSDKIYVDKIFLGADALLKKGVINKIGSGMFAEIASRNKIPVYIIADSWKFTKDKI
                     KIEQRSLNEVWDKSPKNIKIKNPAFEFIKRKYISGIITELGLMKYKEFLRKIN"
     gene            2882..3739
                     /locus_tag="PLU55_04175"
     CDS             2882..3739
                     /locus_tag="PLU55_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HPJ87285.1"
                     /translation="MLEALINPRKAEKGPWKMFFVGLVYASLSLLLVHWFFSGDPNLS
                     NASGMLVVLFCIMFSFPFMYYMIKTEGREDEEVEGLYNVWQAHKDAIYAFMGLFLGFV
                     VAFSFWNIVLQDANLLNFQIQTYCQINRPSDVQGCVQEYSSAKVNLTGSSINGVRLLS
                     IIENNVYVMIFTLIFSLIFGAGALFILVWNASVIGAAVGIFTRYNVSEIPLGIARYAI
                     HGFPEIAAYFITALAGGIFGVGLIRHGLRDKRFLKIVENVILLIFLALVILIIAALLE
                     VYITPLIFR"
     gene            3771..4769
                     /locus_tag="PLU55_04180"
     CDS             3771..4769
                     /locus_tag="PLU55_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 4"
                     /protein_id="HPJ87286.1"
                     /translation="MEPLIVMTVFLSFFCTYLTLPFWIKKAKQIGLLWEDMNKPRREK
                     NVAGSGGIIVVIGAIIGIFMYIAIQTFYFKSSDGISIQLFAMTSTILLVAGVGLIDDL
                     FGWKKGGLSKRSRIILVMLSAIPLMIINAGESTMLGMDFGILFPLVIIPLGVVGATTT
                     FNFLAGFNGLEARQGIILLSAIGIVTYLTGNSWLTVVALCLVASLLAFYIFNANPAKV
                     FPGDTLTYAIGASFACIAILGNVEKIAIFFFIPYLLETILKLRGGLKKQSFGRPNEDG
                     SLEMPYEKIYGLEHLAIYILKKIKPSKKVYENDVVSLINLFQIIIIIIGFVLFIKF"
     gene            4807..>6295
                     /locus_tag="PLU55_04185"
     CDS             4807..>6295
                     /locus_tag="PLU55_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STT3 domain-containing protein"
                     /protein_id="HPJ87287.1"
                     /translation="MKISGLKEKFNNTIKILERKKILTIITILLLITIIAFTANIRTN
                     NIDKLKDVTTGEYLTADLDAFYFLRLAEVLHENGSYSEYDSMRYPLLKLSFSNELLPY
                     TIVFLYKVIKPLSADITIRYVDVIYPVIFFVLGMLVFFFLILHLTKSRMVALLSSFFL
                     AIIPSYLFRTMAGVSDHEPLGMFAFFLALLVFSISMEKIEKKNSWKMAIILGIITGFF
                     TIFSIAAWSGIASFVLLIIPLTIFLLWITNKRNSEEGKNNLREYLLFYGFWIIAFISL
                     GFIFSYPITAIKGFIFNMRGLIIPLVFVFILTDYILINMKHRKIEKLGKKRIGVSLIL
                     TLFVSIIIALIFNLNLGFSLSDIVSSVLRPFGGGRIGSTVAENALPYTVDIIRQIGAA
                     LFWIFFLGVIFVGIESSKGIGKKKNRIIFSLLWVFMISGILFHRYSTTALFNGNSFLS
                     KLFYIGGMLLFFGYFVWIYFKDNIKIKGNQIIILLYVFFMTLSGIS"
BASE COUNT         2137 a          802 c         1035 g         2321 t
ORIGIN      
        1 agatttcagt ggaactgctt gttctggaaa agtattggtt ttaattcaat ctataattaa
       61 caaatatcct gtagagtttg cttctgcatt aggagcatta ttatctacag ttgtgatttt
      121 ccttttagtt gtatgggcac aaagcttgaa ggttgaaatc cctttaagtt ttggaagaat
      181 aagaggatat ggaattaagt ggccattgaa ctttttctat gcttctgtta taccagttat
      241 tttaactgca gcactagttg caaatatcca attgtttgga gggttacttg aaaattgggt
      301 aggacaccct acattcttag gacacttctc taatgggcag gcagtttcag gatttgcttt
      361 ctggctagga catactaatt tacttgagct aataataaga ggaggattct tgcctagata
      421 tcttattcag ggtataactc atattatatt ttatgttgga cttgctactt tattctctgt
      481 attctgggtt aaaacatctg gaatgggggc aaaggaacaa gcccagaaga tttctgcatc
      541 tggtttacaa atttcaggat ttagacaaga tgttagggtt ttagaaagtg ttttagatag
      601 atacataatg cctctaacag tgatgggagg tgctgcaata gggttgctta ctgctacaac
      661 agatttatta ggtgcattat cttcaggaac agcaatactg ttggtaatta tgattatgta
      721 tcagttctat cagaagatag cagaacaaca tatgacagat atgaatcctg caatgaggaa
      781 gtttattagt aataattaaa aaagatggta aaagaaaaag ctagtttttc atatatccaa
      841 acaagaaggg attttatcta gatggaagaa acaaatttaa aaaaagacaa gaatgcgaaa
      901 gaaggtagtt ttgccccatt gataattttt atgatacttg ctatggttct tgctggacta
      961 tgggataaaa tacctataat aaaaaattca atacattata tccttgaccc aagtgcagga
     1021 gtacttctga attggaaatt aaatcttgga atgttgataa ttgttcttgt aataacaacg
     1081 ataactacaa ttgtccaaaa atatgctaca gaccaaaaaa ctctaaaaga aatgagaaag
     1141 gaacaaaaag aaatgcaaaa acagatgaat gaatttaaga ataatcctga caaattaatg
     1201 gaacttcaaa agaaacagtt tgctatgatg cctaaacaga tgaaattaag tatgagggca
     1261 ataatttata caggtattcc atttatatta ttttttagat ggtttaatga ttatttcatt
     1321 gctgctggaa gtcctagatt ctggttagga ttaagttggt tttggtttta tttgatattt
     1381 gcaatgatat ttggttcttt cttaagaaaa tggtttgatg ttgcttaaat aattttaacc
     1441 ccaaatctta aaaaccatct tattctgatt taaacatggc aaaagaaaat caagaatcgc
     1501 aacaagaact tgtaatgcaa tttcagattt ttgaacaaca aatacgaatg attcaagaac
     1561 aattacaagc agttgaacaa gcaattgtag agttaggaag tttaaatttt ggattagatg
     1621 aacttgttgg taaaaaagat gaagaaatat tatctccaat tggaagagga atatatgcta
     1681 aatccaaact aatatctgaa gaactgttag tagatatagg caataaaaat tttgtaaaaa
     1741 aatcaatacc tgaaacaaaa aagataattc aaaatcaaat taaaaaatta gagaaagcaa
     1801 aagaagagtt aaacaaagaa ttagaaaaga taaatactga attaacagaa gtatttatga
     1861 aacatcaatc ctctcacaaa catgaccaat gtgattgtgg tgaagaagaa tgtgattgta
     1921 aaaaataatt agtttatctt tcttaaaaat tccttatatt tcatcaggcc taattcagtt
     1981 attattcctg aaatatattt tcttttaata aattcaaatg ctggattttt tattttaata
     2041 ttctttggag atttatccca gacttcattt aagcttcttt gctctatttt tattttatct
     2101 tttgtaaact tccatgaatc tgcaattata taaacaggta ttttatttct tgatgctatc
     2161 tcagcaaaca ttccactccc aatcttattt attactcctt tttttaaaag tgcatctgct
     2221 ccaagaaata ttttgtccac gtatatttta tctgatttgt catctttttt tattgcaaag
     2281 gcagctgcag aatctacaaa cattgtaaca tcgataccat tcttcattaa ttctactgca
     2341 gtctttcttc cttgaaataa aggtctagtt tcagtgttat aaactttgaa ttttttgcct
     2401 tttttcttgg aatatattag ggaattaaca acattagtag aatgacagtg agtaaatata
     2461 acatctctgt tattaatcag ttttaaaaca tgtttattta tctctttttg agaaaattca
     2521 aagtgattta atatctcttt tttagatacc cctttttcag caagattaag aacataaaac
     2581 atcattggct ctgttggtct ggaagacatt aatttttctt ttgaatcttt agaaggtaaa
     2641 agaaaataag cttcaagtgc agcccttgca acattcttag ccccttgaat ttctacattt
     2701 tttatttttc ttacaatttc attgaacctc tttttcttat tcatatattc aagaagttgt
     2761 taagatttaa aaatacttct tctaaattca tagaagaaaa atcatacaat caacaataat
     2821 ttttaatcaa atcctcaatt atatacctaa catttaaaaa ttccttttat tttaaaccat
     2881 tatgcttgaa gcacttataa atccaagaaa agcagagaag ggaccatgga aaatgttttt
     2941 tgttggtctt gtttatgcat ctctgtcact tttattagta cactggtttt tttcaggaga
     3001 ccctaattta tcaaatgcat ctgggatgtt agtagttttg ttttgtataa tgtttagttt
     3061 tccttttatg tattatatga ttaaaactga agggagagaa gatgaagaag ttgaagggtt
     3121 atacaatgtt tggcaagcgc acaaagatgc aatctatgca tttatgggtt tatttttggg
     3181 ttttgtggtt gcattttcat tttggaatat tgttttacaa gatgcaaatc tcttaaactt
     3241 tcaaattcaa acttattgtc aaataaatcg tccttcagat gttcaaggat gtgttcaaga
     3301 gtattcttca gcaaaagtaa atctaactgg atcgtcaata aatggtgtta gattgctctc
     3361 tataatagaa aataatgttt atgttatgat atttactctc atattttcgc tcatatttgg
     3421 agcaggagct ttatttattc tagtctggaa tgctagtgtt ataggcgctg cagtagggat
     3481 atttacaaga tataatgtaa gtgaaattcc attaggcata gcaagatatg ctatacatgg
     3541 gttccctgaa atagctgcat atttcattac tgctcttgcc ggaggaatat ttggtgttgg
     3601 tttaataagg catggtctaa gagataaaag atttttgaaa attgttgaaa atgttatttt
     3661 acttatattc ttagcattag taatactcat aatagctgca ctattagaag tatatattac
     3721 accattaata ttcagataaa atttaaaagc tcgaagtata ttcaattata atggagcctt
     3781 taattgtaat gactgttttt cttagttttt tttgtacata tttaacatta cctttttgga
     3841 taaaaaaagc aaaacaaatt ggattattgt gggaagatat gaataagcca agaagagaga
     3901 aaaatgttgc tggttctgga ggaataattg tagttatagg ggcgataata ggtatattta
     3961 tgtatattgc tatacaaaca ttttacttta aatcttcaga tggaatatca atacaattat
     4021 ttgctatgac tagtacaatt ttgcttgtag caggtgtggg attaatagat gatttatttg
     4081 gatggaaaaa aggagggctt tcaaaaagat ctagaataat acttgtaatg ttgtctgcaa
     4141 tacccttaat gattattaat gctggagaat caacaatgct cggcatggat tttggaatat
     4201 tatttccttt agttattatc cccttaggtg ttgttggtgc aacaactaca tttaatttct
     4261 tagcaggatt taatggatta gaagcaagac aagggataat tttgttgtct gctattggaa
     4321 tagttactta cctaacagga aattcttggt taactgttgt tgcgctctgt ttagttgcct
     4381 cattacttgc attttacatt tttaatgcta acccagcaaa agttttccca ggagatacat
     4441 taacttatgc tataggggca tcatttgctt gtattgctat acttggaaat gtagaaaaaa
     4501 ttgcaatatt cttttttatt ccctaccttt tagaaactat tttaaaacta agaggaggat
     4561 tgaagaaaca aagttttggt aggcctaatg aagatggaag tttagaaatg ccttatgaaa
     4621 aaatatatgg attagaacat cttgctatat acatattaaa aaaaataaaa ccaagcaaaa
     4681 aagtttatga gaacgatgtg gtatctctta taaatttatt ccaaataatt ataattataa
     4741 ttggttttgt attgttcata aaattttaac tataaggttt ttaaatgacg tttttcttta
     4801 attctaatga agattagtgg gttgaaagaa aagtttaata atactataaa aatcttagaa
     4861 aggaaaaaaa ttttaacaat aataacaata cttcttttaa taacaattat agcgtttact
     4921 gcaaatataa gaacaaataa tatagacaag ttaaaagatg ttacaactgg tgaatattta
     4981 actgctgatt tagatgcttt ttatttttta agattagctg aggtactcca tgaaaatgga
     5041 agctactctg aatatgattc tatgagatat cctctactta aactctcatt cagtaatgaa
     5101 cttcttccat atacaattgt atttttatat aaagttatta aacctttatc agcagatata
     5161 actatcaggt acgttgatgt tatttatcct gtaatatttt ttgtgttagg catgttggta
     5221 ttcttctttt tgatacttca tctcacaaaa tcaagaatgg ttgctctatt atctagcttt
     5281 ttccttgcaa taattccttc gtatttattt agaacaatgg caggagtttc agaccatgaa
     5341 cctcttggaa tgtttgcttt tttcttagca ctacttgtat tttcaatttc tatggaaaaa
     5401 atagaaaaaa agaattcttg gaaaatggca ataatccttg gtattattac tggtttcttt
     5461 acaatattta gtatagctgc ttggagtggt attgcttcat ttgtattgct cataattcct
     5521 cttacaatct ttttattatg gataactaat aaaagaaaca gtgaagaagg aaagaacaat
     5581 cttagagaat accttttgtt ttatggattt tggataattg catttatttc acttggtttt
     5641 atattttctt atcctattac tgcaataaaa ggttttatct ttaatatgag gggtttaatt
     5701 attcctttgg tatttgtatt tattttaact gactacatct taataaatat gaaacataga
     5761 aagatagaaa aattaggtaa aaaaagaata ggggttagct taatattaac attatttgtt
     5821 tcaattatta ttgctttaat atttaactta aatcttggtt tttctttatc tgacattgta
     5881 tcctctgttc ttaggccatt tggaggagga agaataggct caactgtagc agaaaatgct
     5941 cttccatata cagttgatat tataaggcag ataggtgctg cattattttg gatattcttt
     6001 ttaggcgtta tatttgtggg tattgaatct tctaagggaa taggtaagaa aaagaataga
     6061 ataatatttt ccttgttgtg ggtattcatg atatctggaa tattgtttca tagatattct
     6121 acaacagcat tgtttaatgg aaattctttt ttgagtaaat tattttacat tggaggaatg
     6181 cttctctttt ttggatattt tgtttggata tattttaaag ataatataaa aataaaaggc
     6241 aaccagataa taatattgct atatgtattc tttatgactc tatcaggtat aagtg
//
LOCUS       DAOPCH010000051         6272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_10942_length_6272_cov_4.040373, whole genome shotgun sequence.
ACCESSION   DAOPCH010000051 DAOPCH010000000
VERSION     DAOPCH010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10942_length_6272_cov_4.040373"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PLU55_04190"
     CDS             complement(<1..372)
                     /locus_tag="PLU55_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87288.1"
                     /translation="MRKANIGVIIAILVMIYSFSVIASAEPCNLQISMINQDPYPAIQ
                     GDYVKVLFKIDGIRNTECGTVTFGIKEDYPISLDPNVENPITIKSGVYQKNYASYYLA
                     SYKLRIDSNALDGENPIEVYYS"
     gene            complement(418..>1655)
                     /locus_tag="PLU55_04195"
     CDS             complement(418..>1655)
                     /locus_tag="PLU55_04195"
                     /inference="COORDINATES: protein motif:HMM:NF003078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87289.1"
                     /translation="FGFESDLYYNISDFTKKIALIPCSAKTHEGIPELIMMLCGLSQK
                     YLTDKLKMGKEPKGVLLEVKKEKSNNYIEAILYDGELKKTDEIAIANFNTEKEPIVTK
                     IRILEEIEPLCAKFKPKESVTASTGIRIQLIEKDEVLPGMPFEIYKNNLQELKEKFNK
                     EISDKIKTKNNGIIAKADSLGSIEALLFLLENQNIPVLKAGIGNINKSDVIAAKANME
                     INELDVVIVGFNVGIDEDANELIKDQGKKIKVITDEVVYKILEDLVDWRTQKQKDIEK
                     KRLMELVPICKVKILHQYVFRNTSPAIFGVRIEAGKLKQQMHLIDEKGEKVGKIKNIE
                     SERKSVPEAGEGMEVAVSMPGINFERAMKNRNFMYSDLGESQFRNLKKNKDLLSGAEM
                     KMLQEIAEIKRQEKADWGI"
     assembly_gap    1656..1665
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1666..2196)
                     /locus_tag="PLU55_04200"
     CDS             complement(<1666..2196)
                     /locus_tag="PLU55_04200"
                     /inference="COORDINATES: protein motif:HMM:NF003078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPJ87290.1"
                     /translation="MENQVKIRQPIVTVCGHVDHGKTSILDCLRNSAVQAGEHGGITQ
                     KISFTSYPIAQMKVACPLIDKSGIKVNIPGFLLIDTPGHAAFTNLRKRGGSLADLAIL
                     VVDINEGIKPQTAEVIQILKLHKTPFLIALNKIDNISGWRTNTDKPLKESVESQGSNV
                     RQIFDERYMTLIGSLSS"
     gene            complement(2243..2470)
                     /locus_tag="PLU55_04205"
     CDS             complement(2243..2470)
                     /locus_tag="PLU55_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87291.1"
                     /translation="MVEANYIQEKMAEIQKSEELSNIMGKLLSGKPGYKAVIEKKIIQ
                     VRCPGNCGMIFESPVKFCPECGSKIEWPKKE"
     gene            complement(2563..3687)
                     /locus_tag="PLU55_04210"
     CDS             complement(2563..3687)
                     /locus_tag="PLU55_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPJ87292.1"
                     /translation="MECNRLQLAEKKKEEVKCKAVQEKFQRFMTIDEVSKIFDLDKDE
                     LKKICSNYPMRIPDYYLSLIKEKGDGVYKQCIPSSAEIYDLCGEEDPLHEEPEHQKRK
                     NVPSLLTHRYPDRVLFRISNSCAMYCRFCTRKRKVGDITKNPTWEEMAKAILYIKQHP
                     EIRDVILSGGDPLMLPNKLLEKIIKAVHSIISKRENGIIRIGTRIPCVFPQRITEELC
                     DMLKKYHPIYINTHFNHPNEITLESKRACELLVNAGIPVGNQTVLLKGVNDNPETMKK
                     LMLGLLSIRVRPYYIYQCDTVKGANHFRTRVEKGLEIYKSLRGHTSGLAVPQFVIDAP
                     GGGGKIPLLPEYVQSIDKDKVVLKNYEGNTHEYPQIKEDY"
     gene            complement(3663..4223)
                     /locus_tag="PLU55_04215"
     CDS             complement(3663..4223)
                     /locus_tag="PLU55_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87293.1"
                     /translation="MIQLLSDNEPPPKFQRKKEVKNKGKNLPQLVFSLKIENNGNTLN
                     NSNNKKLEDRIKNNPVNNQYLEDYSALEKSFSKNSTTTSQKKIRFSEINTSNESLSQI
                     NTDYLEINKENSVKNRKNQNFYFKNTEYNFIFFQTFIKDDNKEKKYYLELRETQEKDK
                     IYSLSSFDEEKTTDEREIKWNATDYN"
     gene            complement(4279..4989)
                     /locus_tag="PLU55_04220"
     CDS             complement(4279..4989)
                     /locus_tag="PLU55_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87294.1"
                     /translation="MVVSETFTLGRITLLALADSVNPCEIAVLTMVLVSILLANPRNK
                     KKVLFAGLAFSLAVFIGYLAYGVIIIQFFKVFAEFLRESSRYVYNGLAILAMIIGALN
                     IKDFFFYKAGSFGTEMPIFMRPKVKKIIGNITSPKSAFLIGFVVTLFLLPCTIGPYIV
                     ASGLLSELGTFGAIPWLLYYNLVFILPMIVITLIVYLGLARVEDVSGWKEKNIRKLHL
                     IAGILLFLVGLGIILGWI"
     gene            complement(5016..5300)
                     /locus_tag="PLU55_04225"
     CDS             complement(5016..5300)
                     /locus_tag="PLU55_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HPJ87295.1"
                     /translation="MPRPCKRRRIRGNPQSNYFKPAGILIRELKETELEISEFEAIRL
                     VDFQEISQEEAAKQMQISQPTLSRTLTSARKKIADSIINGKAIKISKDLN"
     gene            complement(5305..5448)
                     /locus_tag="PLU55_04230"
     CDS             complement(5305..5448)
                     /locus_tag="PLU55_04230"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPJ87296.1"
                     /translation="MPNKDKTGPEGKGPKTGRQMGNCEGAEPQRGKRRGLGRNRRFNR
                     RSE"
     gene            complement(5585..5938)
                     /locus_tag="PLU55_04235"
     CDS             complement(5585..5938)
                     /locus_tag="PLU55_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87297.1"
                     /translation="MKRPTKKQIKSNIITLAIIIIILVIAILALSKKPVETDEKEIKC
                     IASKTTLYTQLGCHACESQRELFGNNYKYLNVIDCWYERDKCNEIEYTPTWIINQEKV
                     IGVQTIEKLKELTGC"
     gene            complement(6000..>6272)
                     /locus_tag="PLU55_04240"
     CDS             complement(6000..>6272)
                     /locus_tag="PLU55_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87298.1"
                     /translation="IKKNDSTDIIIRIDDFGGRKGLTIREYVTSDRYTGFTKAGIRIG
                     SADFPKFKEMINSVSEEELAAEAESAPIETKKKPKKPASQEELPDY"
BASE COUNT         1722 a         1006 c          852 g         2682 t
ORIGIN      
        1 tgaataatat acttcaatag gattttctcc atcaagagca tttgaatcta ttcttaattt
       61 atatgaagca agatagtatg acgcataatt tttttgatat actcctgatt ttattgttat
      121 tggattttct acattcgggt caagagaaat aggatagtct tctttaattc caaatgtaac
      181 tgttccacat tctgtgtttc taatcccatc tattttaaat aaaactttaa cataatctcc
      241 ttgaattgca ggataagggt cttggtttat catagaaatt tgtaagttac atggctctgc
      301 tgaagctata accgagaatg aataaatcat aaccaatatt gcaattataa ccccaatatt
      361 tgcttttctc attatgtaaa ttatgtattt ttgtttttaa aactttgtaa ttctaaatca
      421 aattccccaa tcagcttttt cttgtctttt tatctcagct atttcctgaa gcattttcat
      481 ttcagcacca gaaagtaaat ctttattttt cttaagattt ctgaattggg attctcctaa
      541 atcagaatac ataaagtttc tgtttttcat tgctctttca aaatttatcc caggcataga
      601 aacagcaact tccattcctt cccctgcttc tggaactgat tttctttcag attcaatatt
      661 ttttattttt cctacttttt ctcctttttc atctattaag tgcatttgtt gttttaattt
      721 tcctgcttca attcttacac caaatattgc aggacttgta tttctgaaaa catattggtg
      781 aaggattttt accttgcata ttggtactag ttccattaat ctcttttttt caatatcttt
      841 ttgtttttga gttctccagt caaccaaatc ttcaaggatt ttatatacaa cttcgtctgt
      901 tattactttt atttttttac cttggtcttt tattaattca ttagcatctt catctattcc
      961 aacattaaat ccaactatta ctacatccaa ttcatttatt tccatatttg ctttagcagc
     1021 tattacatct gatttgttga tatttcctat acctgctttt agaacaggaa tattttgatt
     1081 ttctagaagg aaaagaagtg cttcaatact tcctaaagaa tctgcctttg ctattattcc
     1141 attatttttt gtttttattt tgtctgatat ttctttattg aatttttctt ttagttcctg
     1201 aagattgttt ttgtaaatct caaatggcat acctggtaaa acttcatctt tttcaattaa
     1261 ctgtattctt atgcctgttg aagctgttac actttctttt ggtttgaatt ttgcacagag
     1321 tggttctatt tcttctaata ttctgatttt agttactatt ggttcttttt cagtgttaaa
     1381 gtttgcaatt gctatttcat ctgttttttt taactctcca tcatataata tggcttcaat
     1441 gtaattattt gatttttctt ttttaacctc caatagaact ccttttggtt cttttcccat
     1501 ctttaattta tctgttagat atttttgact taaaccacaa agcatcatta ttaattcagg
     1561 tattccttca tgggtttttg cagaacaagg aattaatgct attttttttg tgaaatctga
     1621 aatattataa tataaatctg attcaaaacc aaagcnnnnn nnnnngctac ttaatgagcc
     1681 aattaatgtc atgtatcttt catcaaaaat ctgacggaca ttgcttcctt gagattcaac
     1741 gctttctttt aagggtttat ctgtatttgt tctccatcct gaaatattat ctattttatt
     1801 taaagcaatt aaaaaaggtg ttttatgaag tttaagtatt tgaatgactt cagctgtttg
     1861 gggttttatt ccttcattta tatctacaac taaaattgct aaatctgcca gagaacctcc
     1921 tctttttcta agattagtaa atgccgcatg tcctggggta tcaataagta aaaatcctgg
     1981 tatatttact tttattcctg acttgtcgat tagaggacaa gctactttca tttgagcaat
     2041 aggataagaa gtgaaagata ttttttgagt tatgcctccg tgttctccag cttggacagc
     2101 gctatttcta agacaatcta atatagatgt ttttccatga tccacatgcc cacagactgt
     2161 tactattggt tggcggattt ttacttgatt ttccatactc aagatatata agaagtagtt
     2221 aaaaatgttt tcaaatttaa ttttactctt tcttaggcca ttctattttt gaaccgcatt
     2281 cagggcagaa ttttacaggg ctttcaaaaa tcatcccaca attaccagga catcttactt
     2341 gaataatctt tttttcaatt actgctttat aaccaggttt tccagaaaga agtttaccca
     2401 ttatattaga aagctcttcg cttttttgta tttctgccat tttttcttga atataatttg
     2461 cttcaaccat ctttgtatct ccctccttta tttctttctt ttagtatatg aatagaaaat
     2521 ttatttttaa attttttgat aatagaaatt atttaatact tttcaataat cttctttgat
     2581 ttgcgggtat tcgtgagtgt ttccctcata gttcttaaga acaaccttgt ccttgtctat
     2641 actttgaaca tattctggta ataaaggtat ttttcctcct cctcctggag catcaattac
     2701 aaattgtggg actgcgagtc ctgaagtatg tcctcttaat gatttgtaga tttcaagtcc
     2761 tttctcaact cttgtcctga aatgatttgc acctttaact gtatcgcatt gataaatgta
     2821 ataaggtctt actctaatac tcaataaacc taacattagt tttttcatgg tttctggatt
     2881 atcatttact cctttaagaa gaactgtttg atttccaact gggattcctg cattaacaag
     2941 aagttcacag gctcttttgc tttctagagt tatttcattt gggtgattaa aatgagtgtt
     3001 tatgtaaata gggtggtatt tttttaacat atcgcatagt tcttctgtta ttctttgagg
     3061 aaaaacacaa gggattcttg tgcctattct gattattcca ttttctcttt ttgatatgat
     3121 tgagtgtact gcttttatta ttttttcaag taatttatta ggaagcatta aagggtctcc
     3181 tccagaaaga attacatcac gtatttcagg atgttgtttt atatataata ttgctttagc
     3241 catttcttcc catgttggat ttttggttat atctccaact tttctttttc ttgtacaaaa
     3301 tctacaatac attgcacatg agtttgaaat cctgaaaaga actctgtctg gatacctgtg
     3361 agttagaaga gaaggaacat tttttctttt ttggtgttct ggttcttcat gaagggggtc
     3421 ttcttctcca cataagtcgt aaatttcagc agaagatgga atacattgtt tgtaaacccc
     3481 gtcccctttt tcttttatta aagataaata gtaatctgga attctcatag gataattact
     3541 acatattttc tttaattcgt ctttatccaa gtcaaatatc ttagaaacct catctattgt
     3601 catgaatctt tggaattttt cttgcacagc tttacatttt acttcttctt ttttcttctc
     3661 tgctaattgt aatctgttgc attccacttt atttctctct catcagttgt tttctcttca
     3721 tcaaaagagg acaaggaata gattttatct ttttcttgtg tttctcttaa ttccaagtaa
     3781 tatttttttt ctttgttatc atcttttata aatgtttgga aaaaaataaa gttatattcg
     3841 gtgtttttaa aataaaaatt ttgatttttg cgatttttta ctgaattttc tttatttatt
     3901 tccagataat ctgtatttat ttgacttaaa ctctcgttgc tggtgtttat ctctgagaat
     3961 ctaattttct tttgagaagt tgttgtgctg ttttttgaga atgatttttc aagagcagaa
     4021 taatcttcta aatattggtt gtttacaggg ttgtttttta tcctatcttc tagtttttta
     4081 ttatttgagt tgtttagagt atttccatta ttttcaatct ttaaagaaaa caccaattgt
     4141 ggaagatttt tacccttatt tttcacttct ttttttcttt ggaatttggg gggtggttcg
     4201 ttgtctgata ataattgtat catattttac tgttactttt ttatgtgagc tttatttaaa
     4261 aatcttgtta tttaagattt atatccagcc aagaatgatt cctaatccta ctaagaaaag
     4321 caagattcct gcaattaaat gaagttttct gatgttcttt tccttccatc ctgaaacatc
     4381 ttcaactctt gctaatccaa gatatacgat taaggttata actatcattg gcaatatgaa
     4441 aacaaggtta taatacaata accagggtat tgctccaaat gttcctaatt cagaaagtaa
     4501 acctgaagct acaatatatg ggcctattgt gcaaggtaac aggaataatg ttacaacaaa
     4561 tcctattaag aatgcagatt taggagaggt tatatttcca attatttttt ttacttttgg
     4621 tctcataaat attggcattt ctgtcccaaa actacctgct ttatagaaaa agaagtcctt
     4681 tatgtttaat gctcctataa tcatggctaa tattgctaag ccattgtaaa catatctaga
     4741 gctttctctt aaaaattcag caaaaacttt gaagaattgt ataattatta ccccatatgc
     4801 taaatatcca ataaatactg caagtgagaa cgctaatcca gcaaaaagaa cctttttttt
     4861 atttcttggg tttgctaaaa gtattgagac tagaaccatt gtaagaacag ctatttcaca
     4921 gggattaact gagtctgcaa gtgcaagaag agttattctt cctaaagtga atgtttcaga
     4981 tacaaccatt ttgtattctc ttatttattt aattattaat ttaaatcttt cgaaattttt
     5041 attgctttcc cattaattat agaatctgct attttttttc ttgcagaagt aagtgttctt
     5101 gagagtgtag gttgagaaat ttgcatttgt tttgctgctt cttcttgaga gatttcttga
     5161 aaatccacaa gtcttattgc ttcaaattca gaaatttcta attctgtttc ttttaattct
     5221 cttattagaa ttcctgctgg tttaaaataa ttggattggg ggttgcctct aatccttctc
     5281 cttttacacg gacgaggcat tttattattc acttctcctg ttgaatcttc tgtttctacc
     5341 caatcctctt ctttttcctc tttggggttc tgctccttca cagtttccca tttgtcttcc
     5401 tgtttttggt ccttttcctt ctggtcctgt tttgtcttta tttggcattt ttgtattttc
     5461 tcctgtttta gttatgaata tatattcata attatattta aatgttttgg ttttataaga
     5521 aaattaaaaa attatttttt attaagattt taaattttat aatgatttag acatttgtat
     5581 taaattaaca tcctgttaat tcttttaatt tttcaattgt ttgaacacca ataacttttt
     5641 cttgatttat aatccaggtt ggagtgtact ctatttcatt gcatttgtct ctttcatacc
     5701 aacaatcaat tacattaagg tatttgtaat tatttccaaa taattctctt tgactttcac
     5761 aagcatggca tcccaattga gtatatagtg ttgttttaga tgcaatacat tttatttctt
     5821 tttcatctgt ttcaactggc ttttttgaga gtgcaagaat tgctataact aaaatgataa
     5881 ttattattgc gagagttatt atgttacttt ttatttgttt ttttgttggt cttttcataa
     5941 attatataat ttaaaatatg ttttaaattt tttggagagt taaaattctt tagaattaat
     6001 taataatcag gcagttcttc ttgtgaagct ggtttttttg gttttttctt tgtttctatt
     6061 ggggcagatt cagcttctgc agctaattct tcttctgaaa cagaattaat catttctttg
     6121 aatttaggaa aatctgctga gcctatcctt atacctgctt ttgtgaatcc agtatatctg
     6181 tcagatgtaa catattctct tattgtaagc ccttttcttc ctccaaaatc atctattctt
     6241 attattatat ctgttgaatc gtttttcttt at
//
LOCUS       DAOPCH010000052         6117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_11306_length_6117_cov_6.547674, whole genome shotgun sequence.
ACCESSION   DAOPCH010000052 DAOPCH010000000
VERSION     DAOPCH010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11306_length_6117_cov_6.547674"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="PLU55_04245"
     CDS             complement(<1..1378)
                     /locus_tag="PLU55_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPJ87299.1"
                     /translation="MINKIILGDCIEELKKLPDNSVDAIITDPPYGLEFMGKDWDKFK
                     QGLNIGGGTTGEGTPFGRSKPLNSVYQYGLEEKNQLQKFCFDWATECLRVLKPGAYLL
                     SFGGTRTYHRMACGIENAGFEIRDCIMWLYGSGFPKSLNIGKQIDKIQGNEREVIGES
                     KWSQPAKSGHHGGLTDNHINFVENKERFIPIETKGSSEWEGWGTALKPAVEPIVVARK
                     PLSEKNVALNVLKWGTGGINIDECRIATEEETRRIKGGWQNGGYVNGKYDSNKYNAFE
                     SRENNGRFPANIILDEEAGEMLDEQTGNIGCSSDREKYKGSNYGTSMFLGEKPLFNSP
                     TYTDKGGASRFFYCAKASKSERNYGCEELEAKHIDIQQPHNSKDLEERYSMTSKNNHP
                     TVKPIALMEYLIKLVSRENAIVLDPFLGSGTTAIACLKTNRKFIGIEKEPEYIKIAEA
                     RIKPYLEQR"
     gene            complement(1375..1659)
                     /locus_tag="PLU55_04250"
     CDS             complement(1375..1659)
                     /locus_tag="PLU55_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87300.1"
                     /translation="MNEEELKEPKLIWKGTTAEALRLGKVTQEDVDRWNRNDVEGESF
                     SNGYDEGFQKGKLEQKKEDNNILIKYSKLRLYRSKSCQLLIKQLQKEIGK"
     gene            complement(1652..1840)
                     /locus_tag="PLU55_04255"
     CDS             complement(1652..1840)
                     /locus_tag="PLU55_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87301.1"
                     /translation="MKTLKQYLKCVGCESEIYECYEYQQMGFDAGLEFGEEHLSFFVI
                     DKDGKFIVPIYWRGKQDE"
     gene            complement(1940..2314)
                     /locus_tag="PLU55_04260"
     CDS             complement(1940..2314)
                     /locus_tag="PLU55_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87302.1"
                     /translation="MTDTQAELNIGIGNLESEKTFLKPAKVKIVSAKIEDTKKAKKVV
                     FWVQHPDKPEQIGLSSIAYLQERSVKVSGTWLNLDKEGKLQKGSALVVLLQKIGANTI
                     QEAVGKECETELDGQYLVFKAY"
     gene            complement(2320..2547)
                     /locus_tag="PLU55_04265"
     CDS             complement(2320..2547)
                     /locus_tag="PLU55_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87303.1"
                     /translation="MNPHILNLLQYDLAESKRAQEILRHIHFDETALRLIYEGIGFSD
                     IYAKIFEELTIEERDLIFNSIVNNIQINSGR"
     gene            complement(2544..2759)
                     /locus_tag="PLU55_04270"
     CDS             complement(2544..2759)
                     /locus_tag="PLU55_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87304.1"
                     /translation="MKNRNLNKEAMCLGFLSVLISILFLWFGLTFSGILSKIEKIISL
                     SGAICGAGLFLILYLLSDAYDIRGEQP"
     gene            complement(2761..2913)
                     /locus_tag="PLU55_04275"
     CDS             complement(2761..2913)
                     /locus_tag="PLU55_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87305.1"
                     /translation="MNKNPERPKDIDEILTGILNPNSKKKRKLLPEQEAYNKRQKELL
                     KSIFGD"
     gene            complement(2906..3232)
                     /locus_tag="PLU55_04280"
     CDS             complement(2906..3232)
                     /locus_tag="PLU55_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87306.1"
                     /translation="MSSVEDGRIAELEAEKILIKYFDIVRYIGNKRKTFDFECYKKNI
                     KFNVEVKFSNGTCFTIIPKELEADILMLKLKDKWFTFYKNILINKYFDMLWEEKVNKI
                     RRIKNE"
     gene            complement(3264..3401)
                     /locus_tag="PLU55_04285"
     CDS             complement(3264..3401)
                     /locus_tag="PLU55_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007108955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VapB family protein"
                     /protein_id="HPJ87307.1"
                     /translation="METDTTTIQVSSEVWKELNIRKKKGDTFEDVLRKLLKLNKKEVA
                     Q"
     gene            3534..3710
                     /locus_tag="PLU55_04290"
     CDS             3534..3710
                     /locus_tag="PLU55_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87308.1"
                     /translation="MELINYIGLKVKIILKNDYYYIGRVFDADKDSIELIDIKGKRVS
                     ISKDTINTIQEVGE"
     gene            3707..6055
                     /locus_tag="PLU55_04295"
     CDS             3707..6055
                     /locus_tag="PLU55_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPJ87309.1"
                     /translation="MNEEEVKKLSDSEVIEKLKEIARLDMPLEINKQLRYLVNNSNYN
                     LQTLQKQLNFVRKEYNKSQVTDVTDVTDVTDVTDVTNELLKPCVTFVICNSSYLDSLF
                     GDSVLDQIIEVLVRSQKALTFAEIALKINKSEVQVRNVISKNPQYFGKIKPKGKICYT
                     YLLQMIFSELQEKINRIKEKKELEKQKKEMEEIEIKGLEKLALEFKNFWKICKKDPQQ
                     ILISKNVIYINFMALAEFNVSIADKLESNPEETISSFELAIEEEGLISNPRIRIFNIP
                     KDKNFNIEELRVKHLKKLISISGRVVTLSDVRPQVVSAKFECPSCGTIISVSQIEKKF
                     REPSRCSCGRKGGFRLISKEMINTARLILEDLQEKTDSPHSKRLNCFLKEDLLNYENM
                     KMYESGIDLNLVGILTEVPIPIPSGGLSTRMELGFEVISAFQSEEELKAEDLTSEEVE
                     EVIKLTSRIDSEGMDCLTDSFAPEIYGHKYIKQGVCLQLASKRNEVGKKLCKNKPNIL
                     LIGDPGTSKSKIGEFAVSITAGARRSVGGSASAVGLTGAVVRDDYTGGWRLEPGAAVL
                     ARDFYMIDELNNVRDEDKPRLQELLSEHTITFDKATIHTKLKAPAGVLATANPIHGSF
                     KEEEDLVTQFNLSSPIINRFDLIFVIKDQVKESDDEKIAQRMNQREMNDLNVEYDSEF
                     LKRFFVYIKNQPNPIFSKEISQKICKIYAVLRRYKTNTMNINPRVHEAFLHLCKASAK
                     LRLSNEVGEKDIERALEILSHSYFRIPSYNSFKEEITEDVRT"
BASE COUNT         2032 a         1003 c          836 g         2246 t
ORIGIN      
        1 tcctctgttc taaataaggt ttaatccttg cttctgcaat cttgatatat tctggttctt
       61 tctcaattcc aataaacttc ctattagttt ttaaacaagc aatagcagta gttccacttc
      121 ctaagaaagg gtctaataca atagcatttt ctcttgaaac taattttatt aagtattcca
      181 ttaaagcaat tggtttaact gttggatgat tgttctttga agtcatagaa tatctttctt
      241 ccaaatcttt tgaattgtgt ggttgttgaa tatcaatgtg ttttgcttct aattcttcac
      301 atccataatt tctctcactc ttacttgcct ttgcacaata gaagaaacga gatgctccac
      361 ctttatctgt atatgttgga gaattaaata atggtttttc tcctaaaaac atagaagttc
      421 cataattact tcctttatac ttttctctat cacttgaaca accaatatta cctgtttgtt
      481 catccagcat ttctcctgct tcttcatcaa gaattatatt tgctggaaag cgaccgttgt
      541 tctccctgct ttcaaaggca ttatatttat tactatcata tttaccattg acatacccac
      601 cattttgcca cccgcctttt attcttcttg tttcttcctc tgtcgctatt ctacactcat
      661 caatattaat tccaccagtt ccccatttca aaacattcaa agcaacattc ttttcagata
      721 aaggttttct tgcaacaaca ataggttcaa ctgctggttt taatgcagtt ccccatccct
      781 cccattcaga agaacctttt gtttctatag ggataaatct ttctttattt tctacaaagt
      841 taatatgatt atctgttaaa cccccatgat gtccagattt tgcaggttga gaccatttag
      901 attctccaat aacttctctt tcatttcctt gaattttatc aatttgcttt ccaatattta
      961 atgacttagg aaatccagaa ccatacaacc acataatgca atctctaatt tcaaatccag
     1021 cattttcaat tccacaagcc attctatgat aggttcttgt tccaccaaaa gataaaaggt
     1081 atgctcccgg ttttagaact cttaaacatt cagttgccca atcaaagcaa aacttttgta
     1141 gttgattctt ttcctctaac ccatattgat aaacagaatt aagaggttta cttcttccaa
     1201 aaggagttcc ttctcctgtt gttcctcccc ctatatttaa cccctgttta aatttatccc
     1261 aatcctttcc cataaattct aaaccataag gtgggtctgt tattatagca tcaactgaat
     1321 tatctggaag tttctttaat tcttcaatgc aatctcctaa tattattttg tttatcattt
     1381 tccaatttct ttttggagtt gtttaattaa taattgacaa ctcttacttc tatataatct
     1441 taatttagaa tatttaatta aaatattatt atcttctttc ttctgttcaa gtttgccttt
     1501 ttggaaaccc tcatcataac cattactaaa actctctccc tcaacatcat ttctattcca
     1561 tctatctaca tcttcttgag ttacctttcc taatctaagt gcttctgctg tcgttccttt
     1621 ccatattaat tttggttctt tcaattcttc ttcattcatc ttgttttccc ctccaatata
     1681 ttggaacaat aaatttacca tctttatcta taacaaaaaa actcaaatgt tcttctccaa
     1741 attctaaacc agcatcaaaa cccatttgtt gatattcata acattcataa atctcacttt
     1801 cacatcctac acatttcaaa tattgtttaa gagttttcat cttgtttttc ctccgaggtt
     1861 tctaataaat attttcctat aattttcttt aatctctctt tctcttcaat tttaaaacgg
     1921 aaaaataatt ttatgatttt taatatgctt tgaatactaa atattgtccg tcaagttctg
     1981 tttcacattc tttacctact gcttcttgga ttgtattagc tccaatcttt tgtaataaaa
     2041 caactagagc tgaacctttc tgtagttttc cttctttatc caaattaagc catgtaccag
     2101 aaaccttgac acttctttcc tgcaaataag caattgaaga taagcctatt tgctctggct
     2161 tatcagggtg ttgtacccaa aaaactactt tcttagcttt tttagtatct tcaatttttg
     2221 cacttacaat cttcactttt gctggtttaa ggaaagtctt ttctgattct aagtttccta
     2281 tacctatatt tagttctgct tgtgtatctg tcatttgttt tacctccctg aatttatttg
     2341 aatattattt actatactat tgaaaattag atctctttct tctatagtta gttcttcaaa
     2401 aatcttagca taaatatcag agaacccaat cccttcataa attaaacgta gggccgtttc
     2461 atcaaagtga atgtgcctga gtatctcttg tgctctttta ctttctgcaa gatcgtattg
     2521 aagtaggttt aatatgtgtg ggttcatggt tgttctcctc taatatcgta agcgtctgat
     2581 agtaaataaa gtattaaaaa taatccagct ccacaaattg ctccagataa agaaataatt
     2641 ttctcaattt tacttaaaat acctgaaaaa gttaatccaa accataaaaa taagatagat
     2701 attaatacac ttaaaaaacc taagcacatt gcttctttat ttaaatttct atttttcatt
     2761 ttaatctcca aatatacttt taagcaactc cttttgtctt ttattatatg cttcttgctc
     2821 tggaagaagc ttccttttct tcttagagtt tggattcaat attccagtta atatttcatc
     2881 aatgtctttt ggtctttcag gatttttatt cattttttat tctccttatt ttatttactt
     2941 tctcttccca aagcatatca aaatatttgt taattaaaat atttttataa aaagtaaacc
     3001 atttatcttt taattttagc attaaaatat ctgcttctaa ttcttttgga ataattgtaa
     3061 agcaggtacc attagaaaat ttaacttcta cattaaattt tatatttttt ttataacatt
     3121 caaaatcaaa tgtttttctc ttatttccaa tatatcttac tatatcaaaa tattttatta
     3181 atattttttc tgcttctaat tctgcaattc ttccatcttc aacactactc attttttgtt
     3241 acctttttct ttagttgttg ttttcattgt gctacttcct tcttatttaa tttcaaaagc
     3301 tttctaagta catcctcaaa tgtatctcct tttttctttc ttatattaag ttctttccaa
     3361 acttctgatg aaacttgaat tgttgttgta tctgtttcca tattataata ttataattaa
     3421 tagtttataa agattgttat actacactat aacaatataa ttctatgatt ttataggtag
     3481 taactaaaaa gtaatcacac taaatatata aagcaccttt ttttaattaa gatatggaat
     3541 taattaatta cattggtctt aaggttaaga ttattctcaa gaatgattat tattatattg
     3601 gtagagtatt tgatgctgat aaggattcta ttgaattgat tgatataaaa ggaaaaagag
     3661 tttcaataag taaagataca attaatacaa ttcaggaggt tggggaatga atgaagaaga
     3721 agtgaaaaag ctttcagatt ctgaagttat tgaaaaatta aaagaaattg caagattaga
     3781 tatgcctttg gaaataaaca agcaattaag gtatttagtt aataattcta actataattt
     3841 gcaaaccctt caaaagcagt taaatttcgt cagaaaggaa tataataaaa gtcaagttac
     3901 agatgttaca gatgttacag atgttacaga tgttacagat gttacaaatg aactattaaa
     3961 accatgtgta acatttgtaa tctgtaactc gtcctaccta gactcacttt ttggagattc
     4021 tgtacttgac caaataattg aggttttggt aaggtcacaa aaggctctta cattcgctga
     4081 aattgctcta aagattaata agtctgaagt gcaggttaga aatgtgattt ctaaaaatcc
     4141 ccaatatttt ggcaaaatta agccaaaagg caagatttgt tacacatact tgttacaaat
     4201 gattttttct gaattacagg agaaaataaa ccgaataaaa gagaaaaaag agctagaaaa
     4261 gcagaaaaaa gagatggaag aaatagagat aaaaggatta gaaaaattgg ctctagagtt
     4321 caagaatttc tggaaaattt gtaaaaaaga cccacaacag atattaattt caaaaaatgt
     4381 tatttacatc aattttatgg ctttagcaga gtttaatgta tctattgcag acaaattgga
     4441 atctaatcca gaagaaacca tttcttcatt tgaattggct atagaagaag agggattaat
     4501 ttctaatcca agaataagga tatttaatat tccaaaggat aagaatttca atatagaaga
     4561 acttagggtt aaacatctta aaaaacttat atctatttct ggtagggtag ttaccttaag
     4621 tgatgtaaga ccacaagttg taagtgctaa atttgagtgt cccagttgtg gaactattat
     4681 ttcagtatcg caaatagaga agaaatttag agaaccatca agatgttcct gtggtagaaa
     4741 aggtggattt agattaattt caaaagagat gataaataca gcaaggctta ttcttgaaga
     4801 tttacaagag aaaacagata gtcctcattc aaaaagattg aattgttttt taaaagaaga
     4861 ccttctaaat tatgaaaata tgaagatgta tgaatcaggg atagatttaa acttggttgg
     4921 aatattaaca gaagttccta ttcctattcc aagtggagga ttatcaacaa gaatggaact
     4981 aggctttgaa gttatctctg ccttccaatc agaagaagaa ctcaaggcag aggatttaac
     5041 ttctgaagaa gttgaagaag ttattaaact tacttcaaga atagattctg aaggtatgga
     5101 ttgtttaaca gattcatttg ctccagagat ttatggtcat aaatatatta agcaaggggt
     5161 ttgtttacaa ttagcttcca aaaggaatga agttgggaag aagctttgta aaaataaacc
     5221 aaacatctta cttatagggg acccaggaac atctaaatct aagataggtg aatttgcagt
     5281 atctataaca gcaggagcaa gaaggtctgt tggaggaagt gcttctgcag ttggattaac
     5341 tggtgctgtt gttagagatg attataccgg aggttggaga ttagaacctg gtgctgctgt
     5401 tcttgctaga gatttttata tgattgatga attaaataat gttagagatg aagataaacc
     5461 aagacttcaa gaacttttat cagagcatac aataacattt gataaagcaa caatccatac
     5521 taaattaaaa gcacctgcag gagttttggc tacagcaaac cccatacatg gaagtttcaa
     5581 agaagaagaa gatttagtta ctcaatttaa tctttcatcc ccgataataa atagatttga
     5641 tttaatattt gttataaagg accaagttaa agaatctgat gatgaaaaga ttgctcaaag
     5701 gatgaatcaa agagagatga atgatttaaa tgtagaatat gattctgagt ttttgaaaag
     5761 attctttgtt tatattaaaa atcaaccaaa cccaatattc tcaaaagaaa tatctcaaaa
     5821 gatttgtaaa atttatgcag tattaagaag atacaaaaca aatacaatga atataaatcc
     5881 aagagttcat gaagcatttt tacatctttg taaggcttct gctaaattaa gattgtcaaa
     5941 tgaagttgga gagaaagata ttgaaagggc tttggaaatt ctttcacatt cttactttag
     6001 aataccttca tataattcat ttaaagagga gattacagaa gatgtcagaa cttgaacttt
     6061 tgccaccaaa cccaaagaaa gaatgtccct gctgtcacaa gacttattcc aagttat
//
LOCUS       DAOPCH010000053         5699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_12211_length_5699_cov_7.455174, whole genome shotgun sequence.
ACCESSION   DAOPCH010000053 DAOPCH010000000
VERSION     DAOPCH010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12211_length_5699_cov_7.455174"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..588)
                     /locus_tag="PLU55_04300"
     CDS             complement(25..588)
                     /locus_tag="PLU55_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87310.1"
                     /translation="MAKKQTLNQGTCVFIDEVGAIKVRVFNILNVEEDKLVISFVDNI
                     QEQYKIPKKYVRAKRVMIIKDSSGKIYVQNPDNWKKIDLKSKGIKELRFNLQNFSLQE
                     SKASIHRWTLPKDTIAKLAPLFKLLMICIVIGVIGWAALKFGTYVLDVVMKSRVMDCS
                     QLIPQVQIPIGTTNPIINTSITTPIGT"
     gene            complement(588..776)
                     /locus_tag="PLU55_04305"
     CDS             complement(588..776)
                     /locus_tag="PLU55_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87311.1"
                     /translation="MKIKILKYKLHRGPTVRVQSHNKINGKWIENQRYVWHKKVANGE
                     TILMHEVLYHKEKEVERE"
     gene            complement(773..1405)
                     /locus_tag="PLU55_04310"
     CDS             complement(773..1405)
                     /locus_tag="PLU55_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87312.1"
                     /translation="MPSGAISELGAIGGGIITKILGAIGIIVFAIGLFYLVRWIQKGT
                     KKQKSFTITAVIVDLNGVFDFDMLAFVKSEETGMLEMIFKNRKTDSLPPIPKHLVKNG
                     NVLLLNYAPGHYCVIDTFETVYNLQRGKWEIVPFNLGMKKYILSKQREIINKAEAKRM
                     KWEQYAPWITLGIAVLAAVLLTAFLFFVGAKLEAKNIAARSLECLGGTVT"
     gene            complement(1415..1963)
                     /locus_tag="PLU55_04315"
     CDS             complement(1415..1963)
                     /locus_tag="PLU55_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87313.1"
                     /translation="MENQQDSYQNYGGNVEPSDSFLKAMIEVEETLNKFEMETLRRKR
                     LRVDLKTKTKTWVPMAAGVSPICNELGISEILGLLRGKATVIGRLTKKTDQETMNDMF
                     QFDRVLIELFASRADDWELDEELAKALKEACVSLVEDIVFSSRNGFTAMNLKSSYHRS
                     ESSNVNSEVNQIQKRSILGIKT"
     gene            complement(2014..2142)
                     /locus_tag="PLU55_04320"
     CDS             complement(2014..2142)
                     /locus_tag="PLU55_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87314.1"
                     /translation="MVDSLGNSTTFLSILIVISFAVVILTLFKPSVNTNPLGKPHY"
     gene            complement(2312..5341)
                     /locus_tag="PLU55_04325"
     CDS             complement(2312..5341)
                     /locus_tag="PLU55_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87315.1"
                     /translation="MAVKDWFKRKDKNLPQAGSILIKPSEADKFKDSKTPVTVVNTST
                     PSSNLNNVPSGNASSSSGRSSGGKIISTGKTVTLPQSTLETPTLSAGGLEKPVDILVG
                     GGSTGLSPFFSSGGKIISTGKTVTLPQSTLETPTLSAGGLEKPVDIKLNRSGGITVGS
                     RTYVGNAVIPELGNISANQYKNKLRQELMQKGSIPRGTGYGRFSYETQTGIEEISPGE
                     KIIYDKYGNTSAITSEFFGKTFKTIDEYIAAIDRYKKSTESEGVYLGKQSSLETPTKV
                     ITRTIKDKKGNTYTYFADGTYQVKDKKGRVWFGEYDLRTGQILQSGTVEPVTPEDVFT
                     VVSGGTALAKKDKKGNTYTYFADGTYQVKDKKGRVWFGEYDLRTGQILQSGTVEPVTP
                     EDVFTVVSGGTALAKLPLKKALTFTFSNVLTTPVNIAYERVYQPTTNVGKFLKAAATG
                     VAITRVPTLGEAYLADLTKAVITKPKQVVKAAVESPAETLGFGVGAGLLGRGLNLVEL
                     QFRGIKVEKINLPGYGEVFIQKIPKYKDIVWIKGTFAQSEKVGIMEQINRLKGRKERV
                     FVQVSSQGIPTKVFSNTKNMGFEVTQVSNPMRGLYQAPPFKFLEGYKNLINTDYGALS
                     FYASASRRTILPNPLELLRSSSRDVFSTQRPFEYIERTYKGIETPKWISEVANAMKEG
                     KDIPKQHKSLVDKLYKEFLNTPIEYKGKFYKGIEKANLLNYLNLRLRGSGIKLKVYSA
                     LLEYQNKNKVSLAGGAENLSGVVPFGPESQVVRALGTRFFAKSIKDLRKGVKPSRTGK
                     VVELLTGTKRGQKVAMIEGQMVEIQPVRSFEGGKRLPKGKKLSKFKPEEFEPTLVDRL
                     INRRREGVKRPVPRPFVSRYTPREDTYDRNLRRVNDLLRPESRIERVERPRITPRTPR
                     VTLRPRRPRTPRVEPPRVTLRPRYPRIRPPRRKKTPETPRPQKLTPKEIQKIKKLKEK
                     QLKDGYLTMPTVYDELIGRVGRTTTKKISGFEVSRIA"
     gene            complement(5342..5614)
                     /locus_tag="PLU55_04330"
     CDS             complement(5342..5614)
                     /locus_tag="PLU55_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87316.1"
                     /translation="MKNLLIVLTIAVILIGGYFVFFNEQQIEIDCNTFGVIYLKPSES
                     HLASGLANKINGIVVIPEENSLIARSEILDCPNFDYSEYIDTGGLF"
BASE COUNT         1688 a         1034 c          713 g         2264 t
ORIGIN      
        1 attatttaaa ttttttcaaa tggtttatgt tcctatgggt gttgttatac ttgtattaat
       61 tattggattt gtagttccaa taggtatttg tacttgagga attaattgag agcaatccat
      121 aactcttgat ttcattacaa catctaaaac ataagttcca aacttcaatg cagcccatcc
      181 tattactcct ataacaatac aaatcattaa caatttaaat aagggagcta atttagcaat
      241 agtatcttta ggaagagtcc atctatgtat agatgctttg ctttcttgta aagagaagtt
      301 ttgtaaatta aatcttaatt ctttaatccc tttagatttt aaatcaattt tcttccaatt
      361 atctggattt tgaacataaa tctttcctga agaatcttta attatcatta ctctctttgc
      421 tcttacgtat ttcttaggta tcttatattg ctcttggatg ttatctacaa agctaataac
      481 taatttatct tcttcaacat ttagaatatt aaataccctt actttaattg ctccaacttc
      541 atctataaat acacaagttc cttggtttaa agtttgtttc tttgccatta ttctctttca
      601 acctcctttt ctttatgata taaaacctca tgcattaaga tagtttctcc atttgcaacc
      661 ttcttatgcc atacatatct ttggttttca atccatttac catttatttt attatgagat
      721 tgtaccctca cagttggtcc tctatgtaat ttatatttta atattttaat tttcatgtaa
      781 cagtaccccc aagacattcc agagatcttg ctgcaatgtt ttttgcctct aatttagcac
      841 caacaaagaa aagaaatgct gtaagaagta ctgcagccaa aacagcaata cctaaagtta
      901 tccaaggagc atattgttcc catttcattc tttttgcttc tgctttattt attatctctc
      961 tttgtttgga caatatatat ttcttcattc caagattaaa aggaacaatt tcccatttgc
     1021 ctctttgtaa attataaacc gtttcaaaag tgtctatgac acagtaatgt cctggagcat
     1081 aattaagtag aagtacattt ccattcttta caagatgttt tggaattgga ggtaaggaat
     1141 ctgtctttct atttttaaat atcatctcta acatccctgt ttcttcagat ttaacaaaag
     1201 ctaacatatc aaaatcaaat accccattta aatcaacaat aactgcggtt atagtgaatg
     1261 atttctgttt cttagtacct ttttgtatcc atcttacaag ataaaataaa ccaatagcaa
     1321 aaactataat accaatagct ccaagtatct tagttataat tccacctcct atagcaccta
     1381 attctgaaat tgctcctgat ggcatattaa ttatttaagt ttttatacct aaaatacttc
     1441 ttttttgtat ttgatttact tctgaattta catttgaact ttcacttcta tggtatgatg
     1501 attttaaatt catagcagta aaaccatttc ttgaagagaa tacaatatct tctactaaac
     1561 taacacaagc ttcttttaat gcctttgcta attcttcatc taattcccaa tcatctgctc
     1621 ttgaagcaaa taattcaatt aaaactctat caaattgaaa catatcgttc atggtttctt
     1681 gatccgtttt tttagttaat cttccaataa ctgttgcctt acctcttaac aatcctaaga
     1741 tttcagaaat acctaattca ttacaaattg gactaactcc tgcagccatt ggaacccatg
     1801 ttttagtttt tgtttttaaa tcaactctta atctttttct tctcaatgtt tccatttcaa
     1861 acttatttaa tgtttcttct acttcaatca ttgcttttag aaatgaatca gaaggttcaa
     1921 cattaccacc gtagttttga taagagtctt gttggttttc cataatgaga taattaatct
     1981 aagaaagttc tatttttaaa tatagcgagt aaattaataa tgtggttttc ctaatgggtt
     2041 tgtatttaca gaaggtttaa acaatgttaa aattacaact gcaaaactaa taacaattag
     2101 aatacttaag aaagttgttg aattgcctaa cgaatctacc attactagtg aagctgttga
     2161 acctgtatct ctccatgttg ctaaataact ccaattatat ttttattaat gcttttaaca
     2221 tctgtttcca ttataaattt tccaaatatt tatataataa atctccaagg acattattta
     2281 aactttttca aaaagatttt tcaagagaat attaagctat tcttgaaacc tcaaatcctg
     2341 atattttttt agttgttgtt cttccgactc ttccgattaa ttcgtcgtat actgttggca
     2401 tcgttaagta tccgtctttc aattgctttt cttttaattt tttaattttt tgtatctctt
     2461 taggagttaa tttttgaggt cttggagttt ctggagtttt ctttcttctt ggaggtctta
     2521 ttcttggata tctaggtctt aatgtaactc ttggaggttc aactctagga gttctaggtc
     2581 ttcttggtct taatgtaact cttggagttc ttggagttat tcttggtctt tcaactcttt
     2641 caattctgga ttctggacgt aataaatcat ttactcttct aagatttcta tcataggtat
     2701 cttctctagg agtatatctt gaaacaaatg gtcttggaac tggtcttttt actccttctc
     2761 ttcttctatt aatcaaccta tcaactaatg ttggttcaaa ctcttctggc ttaaattttg
     2821 ataatttctt tcccttaggc aatctttttc caccttcaaa acttctgacg ggttgtattt
     2881 caaccatttg tccttctatc atagcaactt tctgtcctct tttagttcca gttaataatt
     2941 caactacttt tcctgttcga gatggtttta ctccttttct taaatctttt attgatttgg
     3001 caaagaatct agtacctaaa gctcttacaa cctgtgattc tggtccaaaa ggaacaaccc
     3061 cagacaaatt ttcagctcct cctgctaaac ttactttatt tttattttga tactctaaaa
     3121 gagctgaata aacttttagt tttattccac ttcctcttaa tctaagattg agataattaa
     3181 gtaaatttgc cttttcaata cctttataaa atttaccttt atattcaata ggggtgttta
     3241 gaaactcttt atataattta tcaactaaag atttatgctg ttttggtata tcttttcctt
     3301 ctttcatagc attcgcaact tctgaaatcc actttggagt ttcaatccct ttgtaagttc
     3361 tttcaatata ttcaaaaggt ctttgtgtag aaaatacatc tctagagctg cttcttaata
     3421 attcaagagg attgggtaat attgttcttc ttgaagcaga ggcataaaaa cttaatgctc
     3481 cataatctgt atttattaaa tttttgtaac cttctaggaa tttaaatggt ggagcttgat
     3541 ataatcctct catagggttt gaaacttgag taacttcaaa gcccatattt ttagtgtttg
     3601 aaaatacttt agttggaata ccttgtgaag aaacttgaac aaatactctt tcttttcttc
     3661 cttttaatct attaatctgt tccattattc caactttttc agattgggca aatgtacctt
     3721 taatccaaac aatatcttta tattttggaa ttttttggat aaatacttct ccatatcctg
     3781 gaagatttat cttctcaacc ttaatacctc taaattgtaa ttcaactaag tttaatcccc
     3841 tccctaataa accagctcca actccaaatc ctaaagtttc ggcagggctt tcaactgctg
     3901 cctttacaac ttgtttgggt ttggttataa ccgctttagt taagtctgcc aaataagctt
     3961 ctcctaaggt tggaactcta gttatagcaa ctcctgttgc tgctgccttt aaaaatttac
     4021 ccacattagt tgttggttga taaacacgtt cataagcaat atttactgga gttgttaata
     4081 cattactaaa tgtaaatgtt aaagcctttt ttaaaggaag tttagccaat gctgttccac
     4141 cactaacaac cgtaaataca tcttcaggag ttactggttc aactgttcca gattgtagaa
     4201 tttgtcctgt tcttaagtca tactctccaa accaaactct tcctttctta tcttttacct
     4261 gataagttcc atctgcaaaa taagtatatg tatttccttt cttgtctttt ttagccaatg
     4321 ctgttccacc actaacaacc gtaaatacat cttcaggagt tactggttca actgttccag
     4381 attgtagaat ttgtcctgtt cttaagtcat actctccaaa ccaaactctt cctttcttat
     4441 cttttacctg ataagttcca tctgcaaaat aagtatatgt atttcctttc ttgtctttta
     4501 ttgttcttgt tataaccttt gttggagttt ctaatgaact ttgttttcct aaataaactc
     4561 cttctgattc tgtactcttc ttatatctat ctattgcagc aatatattca tctattgttt
     4621 taaatgtttt accaaaaaat tctgaagtaa tagctgatgt atttccatat ttatcataaa
     4681 taattttttc tcctggagaa atctcttcta tccctgtttg agtttcataa gaaaatcttc
     4741 catatcctgt tcctcttgga atagatcctt tttgcattag ttcttgtctt aacttatttt
     4801 tatattgatt tgctgaaata tttcctaatt ctggaattac tgcatttcca acatatgttc
     4861 tacttcctac tgttattcct ccgcttctat ttaattttat atcaactggt ttttctaagc
     4921 ctccagcaga taatgttggg gtttctaaag tagattgagg aagcgttaca gtctttcctg
     4981 tagaaataat ctttcctcca ctactaaaaa aaggagataa acctgtactt ccaccaccaa
     5041 caagtatatc aactggtttt tctaagcctc cagcagataa tgttggggtt tctaaagtag
     5101 attgaggaag cgttacagtc tttcctgtag aaataatctt tcctccacta cttcttcctg
     5161 aggatgaaga agcatttcca gaaggaacat tgtttaaatt agaacttgga gtagatgtat
     5221 ttacaactgt cacaggtgtc tttgaatctt tgaatttatc tgcttcagat ggtttaatta
     5281 gaatacttcc agcttgaggc aaatttttat cttttctctt gaaccaatct ttaacagcca
     5341 tttaaaaaag tcctccagta tctatatatt cagaataatc aaagttagga caatctaata
     5401 tttcacttct tgcaatcaaa gagttttctt caggtataac aacaatacca ttaattttat
     5461 ttgctaatcc tgaagcaaga tggctttcag atggtttaag ataaataact ccaaaggtat
     5521 tacaatctat ttcaatttgt tgttcattaa agaaaacaaa gtatcctcca ataagaatta
     5581 ctgctattgt taaaacaatc aataaatttt tcattttaat tacctcctta tacttatttt
     5641 aacatattta aactttttta aattaaaatt ttcttcgcat ataaattgga ttaagagaa
//
LOCUS       DAOPCH010000054         5615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_12434_length_5615_cov_4.915288, whole genome shotgun sequence.
ACCESSION   DAOPCH010000054 DAOPCH010000000
VERSION     DAOPCH010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12434_length_5615_cov_4.915288"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..850
                     /locus_tag="PLU55_04335"
     CDS             227..850
                     /locus_tag="PLU55_04335"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HPJ87317.1"
                     /translation="MFYLAEVEDYVRVEPKLFGLPTVEAVDQQLREIYSNYYDKELGR
                     SVAVIEVLEVGEGIIIPGDGATYYNSRFKLLVWKPELQELVYGEIAEITKFGAFIDMG
                     SMRGMIHISQTMDDFVSFSKTNVLSGKVSKRNLKQGDMCMARVVAISHKGEEPKIGLT
                     MRQPGLGKLEWIKEDLIKKEKEAEKAERIAAKGEGKSEKKAKGEKKK"
     gene            852..1046
                     /gene="spt4"
                     /locus_tag="PLU55_04340"
     CDS             852..1046
                     /gene="spt4"
                     /locus_tag="PLU55_04340"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HPJ87318.1"
                     /translation="MAKPKACKLCSVILEEGGDKCPNCGSKELTEGFKGRAVIINPEK
                     SEIAKKLNLKDKGNFAIKTR"
     gene            1049..1396
                     /locus_tag="PLU55_04345"
     CDS             1049..1396
                     /locus_tag="PLU55_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87319.1"
                     /translation="MEIKEIKETKNPVFERKEIQAIIFAESAPTKKDVTTALAKKLSA
                     SEDAIKIKGIYGKFGTKEFRIEANIYKSKEEKNKIERKTKKEIEEEKKEAEAAKKATQ
                     EAKKAKEAENKTE"
     gene            1414..1626
                     /locus_tag="PLU55_04350"
     CDS             1414..1626
                     /locus_tag="PLU55_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011250646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HPJ87320.1"
                     /translation="MAKKIVKKGKKKHVNKPTSKKYAKYKIEGTKVTRLSKFCPRCGP
                     GIFLMNSKGRLYCGKCHYTEFEGKKA"
     gene            1675..2070
                     /locus_tag="PLU55_04355"
     CDS             1675..2070
                     /locus_tag="PLU55_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPJ87321.1"
                     /translation="MVKKKVLIKDCEILPVLTVKLEDDCVTVAKKLHDFQERRIFVVN
                     DKKMPVGIISIVDINDRLVARGKNPNKTKAKDIMTYPVNIVVDLNDSADEVLKKMVRK
                     DNYYIPVTDKGVIKGLITYAGIMKKLSPK"
     gene            2073..2900
                     /locus_tag="PLU55_04360"
     CDS             2073..2900
                     /locus_tag="PLU55_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87322.1"
                     /translation="MASNLEKITEDYTPRNYSEGLEGMSNQEIISYQFPALYKFTNSI
                     KDKKRKKEMEQEINRLFYNYVESIKKYAPATGRAVALEKSYDLVPDEEEIENKNPKEV
                     LEKRLYSSPGRHYQYVNGIVGNKNKSKDIKKEIGKAAKYAIKEGWPIIGAGAGWVIAN
                     RKYKKSGNIIKRANKAFDNKWWQYLGPRGVIRYSADASKFMGGEMLRQGWKLIRKPAA
                     YAIGAYAAYKVISYLIKRKKEKKLENEEIEKLENLRNEMLAQKQFYYAPIQEMREAA"
     gene            2901..3479
                     /locus_tag="PLU55_04365"
     CDS             2901..3479
                     /locus_tag="PLU55_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87323.1"
                     /translation="MVSKKLKAKYSFEKSKRAAKTIYPLFKKETISDFTNEILREDED
                     LRRRVMLNPEYRNILEHKVQEVFKEYQGILKGSKLVDKWDRVTSTVGLVGDAVGPFSG
                     GIGNLISAGEEIPELIPKVFYGLYYAKKTKDWKAIPYWTAMEAASFIPYIGDAIDMSN
                     IYINRARKATKEKVKKEMKKILPSKLELKVAA"
     gene            complement(3510..3869)
                     /locus_tag="PLU55_04370"
     CDS             complement(3510..3869)
                     /locus_tag="PLU55_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87324.1"
                     /translation="MVELTDTIKKIILGLGVVGISYSSLEAQTRTGSDYIRGSKPDKY
                     LKDSNYNSCNFNTKKYLNSKKYKPQAYQNRDDCNFNKKKHLDSKKYKPQAYENKDDCI
                     SRSMKKYSREYNRLKRK"
     gene            4052..4591
                     /locus_tag="PLU55_04375"
     CDS             4052..4591
                     /locus_tag="PLU55_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87325.1"
                     /translation="MNNSNKAIIFLLVIILVAVVFFGIKLIGESNQAFSGSAIKEVEP
                     IQVVKHEIYEQEVPETVEEEIIEKPKESPTEKQFETFKVERLDQNSGQALIFYEINPE
                     DIRCPISEHGPIIFFDETKTYLGYEELKDFLNEYDIVQLLAFGGDNTGALQNIRTETG
                     VSIMVKFSRADESEEPTYC"
     gene            complement(4596..5108)
                     /locus_tag="PLU55_04380"
     CDS             complement(4596..5108)
                     /locus_tag="PLU55_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HPJ87326.1"
                     /translation="MQNYKITLLKGDITKVKADAIVNAANSGMLEGGGVDGAIRYVAG
                     KSLLEECIKLRKEKIPDGLKTGESIQTKAYNLPAKIIIHTVGPKYYSEDIGLLKNCYI
                     NCLKLAEENNCESIAFPAISTGAYGCPIEKSAKIVKEVLSNYIARSLKEVILVLYSQK
                     DYDVYNKEFA"
     gene            complement(5117..>5615)
                     /locus_tag="PLU55_04385"
     CDS             complement(5117..>5615)
                     /locus_tag="PLU55_04385"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HPJ87327.1"
                     /translation="IALAELEKKGILKSIVTQNIDNLHQKAGSENVFEFHGTMKTVTC
                     INPRCMHKYKTEELDLTKLPPKCEKCGSVLKTDICFFEENPPEPAKSLSFQEASKTDV
                     MIIVGTSGVVHPAALLPVTAKENGAKIIEINPDSSLLTIYGTTDIFLQGEAGEVMKKI
                     MEEME"
BASE COUNT         2310 a          731 c          907 g         1667 t
ORIGIN      
        1 aaaaaatata tattcaaagt aaaaatgtag ataatacaat agtcaaattt gcaagaaaaa
       61 atactgaaat aattatagcc actctagata aagaaatgag aaaaaaaata aaaaataaaa
      121 aagtgactat aagaagcaaa aaaataattg aaatcattta atatcttcaa ataagaatca
      181 ctaagaaacc tttttaaagc aatttttact ctaattttta aataaaatgt tttacttggc
      241 tgaagttgaa gattatgtaa gagttgagcc gaaattgttc ggtttaccaa ctgtggaagc
      301 tgttgatcaa caacttagag aaatctattc aaattattat gataaagaac taggaagatc
      361 agttgctgtt atagaagttc ttgaagtagg agaaggaatt attattcctg gagatggtgc
      421 aacttattat aattcaagat tcaaattact tgtatggaaa ccagaattac aagaactagt
      481 atatggtgaa atagcagaaa taacaaagtt cggagcattc atagatatgg gttctatgag
      541 agggatgatt cacataagcc agacaatgga tgattttgta agcttcagta aaacaaatgt
      601 tctttcagga aaagtttcaa aaagaaattt gaaacaagga gatatgtgta tggcaagagt
      661 tgttgcaatc tctcacaaag gagaagaacc taaaataggt ttaacaatga ggcaaccagg
      721 attaggtaaa ttagaatgga taaaagaaga tttgattaaa aaagaaaaag aagctgaaaa
      781 agctgaaaga atagctgcta aaggtgaagg aaaatcagaa aagaaagcaa agggagaaaa
      841 aaagaaataa aatggcaaaa ccaaaagctt gcaaactctg ctcagtaatt cttgaagagg
      901 gaggagataa atgtccaaac tgtggttcaa aagaattaac agaaggtttt aaaggacgtg
      961 cagttataat aaatcctgaa aaatcagaaa ttgcaaaaaa actaaattta aaagataaag
     1021 gaaactttgc aataaaaact agataaaaat ggaaataaaa gaaattaaag aaacaaaaaa
     1081 tcctgttttt gaaagaaaag aaatacaagc aatcatattc gcagaaagtg ctccaacaaa
     1141 aaaagatgtt acaacagctc ttgcaaaaaa actttcagct tctgaagatg caataaaaat
     1201 taaaggaatc tacggtaaat ttggaacaaa agaattcaga atagaggcaa atatttacaa
     1261 atcaaaagaa gagaaaaaca aaatagagag aaaaacaaaa aaagaaattg aagaagaaaa
     1321 gaaagaagct gaagcagcaa agaaagcaac acaagaggca aagaaagcta aagaagctga
     1381 gaataaaaca gaataaaata aataacaatt aaaatggcaa aaaaaatagt aaaaaaagga
     1441 aagaaaaagc atgtaaacaa acctactagc aaaaaatatg ctaaatataa aatagaagga
     1501 acaaaggtta caagactttc aaaattctgt ccaagatgtg gaccaggaat atttttaatg
     1561 aattctaagg gtagattgta ttgtggtaaa tgccactata ctgaatttga aggtaaaaaa
     1621 gcttaaatca taaaagaatt taaaaacttc aaatttcata tttctttata aaagatggta
     1681 aaaaagaaag ttctaattaa agattgtgaa atcttaccag tattaactgt taaacttgaa
     1741 gatgattgtg ttactgttgc aaaaaaactt cacgattttc aggaaagaag aatttttgtt
     1801 gtcaatgaca aaaaaatgcc agttggaata atatcaattg tagatataaa tgatagatta
     1861 gttgcacgcg gaaaaaatcc caataaaaca aaagcaaaag atattatgac ttaccctgta
     1921 aatatagtag tagatttaaa tgattctgct gacgaagttt tgaaaaaaat ggtgcgcaaa
     1981 gacaattact atattcctgt tactgataaa ggagtgataa aaggacttat cacttatgct
     2041 ggtattatga aaaagttgag tcctaaataa aaatggcaag caatttagaa aaaataacag
     2101 aagattatac tccaagaaat tattctgaag gattagaagg tatgagtaat caagaaatta
     2161 taagttacca atttcctgca ttatacaaat ttacaaactc tataaaagac aaaaaaagaa
     2221 aaaaagagat ggaacaagaa ataaatagat tattctataa ttatgttgaa tcaataaaaa
     2281 aatatgctcc tgcaacaggg agagctgttg ccctagaaaa gtcttatgat ttagttcctg
     2341 atgaagaaga aattgaaaat aaaaatccta aagaagtgct tgaaaaaaga ttatactctt
     2401 ctccaggaag gcactatcaa tatgttaatg gaattgttgg aaataaaaat aaatccaaag
     2461 acataaaaaa agaaataggt aaggctgcta aatatgcaat aaaagaagga tggccaataa
     2521 taggagcagg tgcaggatgg gttattgcaa atagaaaata taagaaaagc ggaaacataa
     2581 ttaaaagagc aaacaaagca tttgataata agtggtggca atatcttggc cctagaggag
     2641 taattagata tagtgcagat gcaagtaagt ttatgggagg tgagatgtta agacaaggat
     2701 ggaaattaat tagaaaacct gcagcttatg caataggggc ttatgctgcc tataaagtta
     2761 tttcatattt aataaaaaga aaaaaagaga aaaaattgga aaatgaagaa attgaaaaat
     2821 tagaaaatct aagaaatgaa atgcttgctc aaaaacaatt ttattatgct ccaatacaag
     2881 agatgaggga agcagcttaa atggtaagca aaaaactaaa agcaaaatac tcatttgaaa
     2941 aaagtaaaag agcagcaaag acaatctatc ctttatttaa aaaagaaact atttctgatt
     3001 tcacaaatga aattttaaga gaagatgagg atttaagaag aagagttatg ctaaatcccg
     3061 aatacaggaa tattttggaa cataaagtgc aagaagtttt taaagaatat cagggaatat
     3121 taaaaggatc taaattagtt gataaatggg atagagtaac cagtactgta ggacttgtag
     3181 gagatgctgt aggccctttt tctggaggaa taggaaattt aatttcagca ggtgaagaaa
     3241 tacctgaact aataccaaaa gtattttacg gactatatta tgctaaaaaa accaaagatt
     3301 ggaaagcaat accctattgg acagcaatgg aagcagcatc ttttataccc tatattggag
     3361 atgcaataga tatgagtaat atctacataa acagagcaag aaaagcaaca aaagaaaaag
     3421 taaaaaaaga aatgaaaaaa attctaccat ctaaattaga actaaaagtt gctgcttaat
     3481 ctctagttta tttagtcaga aattcttaat tattttcttt ttagtctatt atattcccta
     3541 ctatatttct tcatacttct tgaaatacaa tcatccttat tctcatatgc ttggggttta
     3601 tacttcttag aatcaagatg cttttttttg ttaaaattac aatcatctct attctgatat
     3661 gcttggggtt tatacttctt agaattaaga tacttctttg tgttaaaatt acaagaatta
     3721 taattagaat ccttaagata tttgtctggt tttgacccac gaatataatc agaacctgtt
     3781 cttgtctgag cttccaaaga actataagaa attccaacta ctcctaaacc caaaattatc
     3841 ttcttaattg tatcagttaa ttcaaccatg ttaaaatcca cttaaagaac tatttaaaga
     3901 tgattgtata aaaatataat ctatggaaaa tatggtttat actaaaatcc aagaaactta
     3961 aatctaaaat ttaaaatgac ttcatttttt cttaacaatt ttaattaatt ttttgaggta
     4021 attttataaa taagaagctc tctttaattt tatgaataat tcaaataagg caattatttt
     4081 tctattggtt ataattttag tagcagttgt tttcttcgga attaaactaa ttggagaatc
     4141 caaccaagcc ttttctggtt ctgcgataaa agaagtagaa ccaatacaag ttgtcaaaca
     4201 cgaaatctat gaacaagaag ttccagagac ggttgaagaa gagattattg aaaaaccgaa
     4261 agaatctcca actgaaaagc aatttgaaac atttaaggta gagagattgg accaaaattc
     4321 tggacaagca ctaatttttt atgagataaa tcctgaagat ataagatgcc caataagtga
     4381 acatggacca attatctttt ttgatgaaac taaaacttat ttagggtatg aagaacttaa
     4441 agatttttta aacgagtatg atatagttca attattagca tttggaggag ataatactgg
     4501 agcacttcaa aacataagaa cagaaactgg agtttctatt atggttaaat ttagcagagc
     4561 agatgagtca gaagaaccta catattgttg atttcttacg caaattcctt attataaaca
     4621 tcataatcct tttggctata caaaactaaa attacttctt ttaaacttct tgcaatataa
     4681 tttgacaaaa cttctttaac tatcttagca gatttttcaa taggacaacc atatgctcca
     4741 gtagatattg ctggaaatgc gatactctca caattatttt cttcagctaa ctttaaacaa
     4801 ttaatataac aatttttcaa taaaccaata tcttcagaat aatattttgg accaactgta
     4861 tgaattataa tttttgctgg taaattatat gctttagttt gtattgattc tcctgtttta
     4921 agaccatctg gaattttctc ttttctcaat ttaatgcatt cttcaagtaa agattttcct
     4981 gcaacatatc ttattgctcc atcaactcct ccaccttcta acattcctga atttgcagca
     5041 tttacaattg cgtctgcttt aactttagtt atatctcctt taagtagagt aattttataa
     5101 ttttgcatag aaaaattcac tccatttcct ccataatctt tttcataact tctccagctt
     5161 ctccttgtaa aaatatgtct gtagttccat atatagttaa caaagaacta tctggattaa
     5221 tctcaattat ctttgcacca ttttctttag cagttactgg gagaagcgca gcaggatgga
     5281 ctactccaga agttccaaca attatcataa catctgtctt tgatgcctct tgaaaagata
     5341 aggacttagc tggttctgga ggattttcct caaaaaaaca aatatctgtt tttaaaactg
     5401 aaccgcactt ttcacatttg ggaggtaatt ttgttaaatc aagttcttct gttttgtatt
     5461 tatgcatgca tctaggattt atgcatgtta cagttttcat tgtgccatga aattcaaaaa
     5521 cattttcact tcctgccttt tgatgcaaat tatctatatt ctgagtaact attgatttta
     5581 agattccctt tttttcaagt tctgcaagtg caata
//
LOCUS       DAOPCH010000055         5556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_12563_length_5556_cov_4.324668, whole genome shotgun sequence.
ACCESSION   DAOPCH010000055 DAOPCH010000000
VERSION     DAOPCH010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12563_length_5556_cov_4.324668"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLU55_04390"
     CDS             complement(<1..695)
                     /locus_tag="PLU55_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPJ87328.1"
                     /translation="MRYLFILGRNIPLSIAEIKSFLKRTSNNILEEKLIGNALLLELS
                     ETLDAGTVDLLGGTLGIGIVLCNVKDIDKREIYFGTENNFNYVIWDFSPHTTVVSEYL
                     KRRFRSEELRAVEKQFGGSIKSQDKNIIRKPSSSIIHEEYFVFENLFGRVIQKCNYKE
                     IEKRDMQKPVRREELSISPRLAKMMINLSEVKEGETLLDCFCGIGVVMIEALNMDINA
                     VGIDKDVNAIEGA"
     gene            783..2306
                     /locus_tag="PLU55_04395"
     CDS             783..2306
                     /locus_tag="PLU55_04395"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPJ87329.1"
                     /translation="MRNPRVLNCTPELIINIGEIGIKSDAIDLPELEINASTGGLAPI
                     PSTYTRAIFKDGRIDLAVCSPKWESSLKKFNDLSTIEIKKMQNILQDKIYLISHPSFN
                     KVKIEGTNTKMYDDSWRFQAKDRAIAFSAGIINNVIPIFRPDIIWLHDWMVGPVAPVA
                     KAMGIKVVSTGHNPLFTELASFDEMVYKGIELRDSEDNQYTPSKYYYITKGKFDFMAS
                     EVNSSDDFTTVSNGYLQRILNGGFDDLAPQVIDAIKKKNKVKHPDGRPRVHGYPNPLK
                     KDGSDLLDSIKLDGLEATILKRKHEGEEIRHSTGLKEGGILLNLTNRLSEHKNPRLQL
                     ETLMYFAEKYDLRYLINANGEQKYVDKALTTALESRGYAAYSKFDGNLEKRAIRTDNA
                     YGLMTPNNEPCGGQNINYPAEGTLIIGHQIDGLIDSVHELNIEKSTGNGFPFKNNNKE
                     GLEYGIVKMKEFAALEDKLRYNQLIRIAEETLKNNSSTARAKQLIEEVFLPLYEEKI"
     gene            complement(2334..4145)
                     /locus_tag="PLU55_04400"
     CDS             complement(2334..4145)
                     /locus_tag="PLU55_04400"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPJ87330.1"
                     /translation="MAQSNINTDANIPEMDLDKNYSPKKFAVPEAPTSEKDKSGDKIK
                     KELDKLKGYILKKYPFTKAISILPPQSIKDFIEEETENLNKEQIEKLQKKVHLNIIIP
                     DEKVKDIYKIKKDIIEQIEKSKQEIWIYMRTPSEIWEICTDQKFELYAAMAMSFPLHD
                     KGILGAMRVTEIHKSLVLQKFEKYVVSYVLGGSLIRGEASKDSDVDVFVIINDTDVKR
                     MPRLELKERLRGIIYQYVGEASALAGVNNKLEPQIYLLTDFWEAVKDAHPVMFTFIRD
                     GVPIYDRGTFMPWKALLKMGKLKPSPEAIDMFMSMGDGVIPRSKRTLLSDIFVNIFWG
                     ITTPAQALLMLNGCPPPNAKKELVRDFKREFLDTKMIEKKYIDFMEKVINIWRDYEHE
                     KIKEISGTEIDKLLKETEGFLKRLKELRTQIDKKAQEKIIEKIYSDTINLLSAIFGKK
                     SQQQLVLEFEKNLVKKGKMTQQHLRILKNIIAARTEFKKGKLDAHKVDNARKEASTLI
                     NDLIEFSQRRDMALLEKRRMKIKFKQGSGELLLSGENAFLFRQNKVFKITSKLEDSNM
                     EEVSKSREDQKNKKDVGFNSKVFDVLKKELGEFEVIL"
     gene            complement(4253..4690)
                     /locus_tag="PLU55_04405"
     CDS             complement(4253..4690)
                     /locus_tag="PLU55_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87331.1"
                     /translation="MSVIKIFEEFLKEGIVKKISIDKNRANNIYLESGRKFKLLQKKI
                     DKLGVDDESANDYIEDCYNILIFLIRAKMLEEGYSSSGQGAHEAEVSYSRKLKFNESD
                     IELLDKLRHFRNGILYYGKRFDKEYAEKIIEFTKKKFKLLNKL"
     gene            complement(4687..5226)
                     /locus_tag="PLU55_04410"
     CDS             complement(4687..5226)
                     /locus_tag="PLU55_04410"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPJ87332.1"
                     /translation="MDTYKLKFTQLEQEIFSFLCLRAGEKLSQREIAQSLKVSPTAIA
                     KSLKKLLDINFARIEKTKTINFISLNRDKPLVIELKRAENLKNIYLSGLSDYLGENLA
                     GSTIILFGSYARGEDTNTSDIDIAIIERKEKKINLAPYEKRLNKKININFYNSWNSIH
                     ISLKNNILNGIVLHGGVNL"
BASE COUNT         1937 a          919 c          685 g         2015 t
ORIGIN      
        1 cttgctcctt caattgcatt tacatcttta tctattccaa ctgcatttat atccatattt
       61 aaagcctcaa tcattaccac acctattcca cagaaacaat ccaataaagt ctctccttct
      121 ttaacttcag aaagatttat catcatttta gcaagtcttg gagaaatact taattcttcc
      181 ctcctgacag gtttttgcat gtcccttttt tcaatttcct tgtaattgca cttctggata
      241 actctcccaa acaaattctc aaaaacaaaa tattcttcat gaataatact tgaagaaggt
      301 tttctaataa tattcttgtc ttgagatttt atacttccac caaattgttt ctcgacagcc
      361 ctaagctctt ctgacctaaa tcttcttttc aaatattctg aaacaacagt agtatgtgga
      421 gaaaaatccc aaataacata attaaaatta ttctctgttc caaaataaat ttctctttta
      481 tcaatatcct taacattaca caaaacaata cctatcccta atgttcctcc aagcaaatca
      541 actgtccctg catctaaagt ttcagacaat tctaaaagca aagcattacc tataagtttt
      601 tcttcaagaa tattattaga agtcctctta agaaaacttt ttatttctgc tattgaaagc
      661 ggaatgtttc ttccaagaat aaataagtat ctcataaaaa atccataaac ctctactaaa
      721 taaatgtttt gtatatattt tatatgtgat aaatttataa actgaaaaaa gacatctaaa
      781 atatgagaaa cccaagagta ttaaattgta cacctgaatt aataataaac ataggggaaa
      841 taggaattaa gtcagacgca atagacctcc cagaactgga aataaatgct tcaacaggag
      901 gattggctcc aattccctca acttacacaa gagcaatatt caaagacgga agaatagacc
      961 ttgcagtttg ttctccaaaa tgggaaagtt ctttaaaaaa attcaatgac ttgagcacaa
     1021 ttgaaataaa aaaaatgcaa aatattctac aagacaaaat atacttgata agtcacccat
     1081 catttaacaa agttaaaata gagggaacaa acacaaaaat gtatgatgat tcttggagat
     1141 ttcaagctaa agatagggca attgcatttt ctgctggaat aataaataat gttattccaa
     1201 tattcagacc agatataatt tggttacatg attggatggt tggtccagtt gcacctgttg
     1261 caaaagctat gggaataaaa gttgtttcaa caggacataa tccccttttc actgaacttg
     1321 catcttttga tgaaatggtt tacaaaggaa tagaattaag agattctgaa gacaaccaat
     1381 acactccaag caagtattat tatataacta aaggaaagtt tgattttatg gcatctgaag
     1441 taaattcttc tgatgatttc acaacagtta gtaatggtta ccttcaaaga atactcaatg
     1501 gaggatttga tgatttagca ccacaagtaa ttgatgcaat aaaaaagaaa aataaagtaa
     1561 aacatccaga tggaagaccg agagtccatg gatatccaaa tcctttaaag aaagatggaa
     1621 gtgaccttct tgatagtata aaattagatg gtttagaagc aacaatacta aaaagaaaac
     1681 atgaaggaga agagataaga cattcaacag gattaaagga aggaggtata ttactaaatc
     1741 tcacaaacag attatctgaa cataaaaacc caagattgca attagaaact ttaatgtatt
     1801 ttgcagaaaa atatgattta agatatttaa taaatgcaaa cggagaacaa aaatatgttg
     1861 ataaagcatt aacaactgca cttgaaagta gaggatatgc tgcttattca aaattcgacg
     1921 gaaatctaga aaagagagca ataagaacag ataatgcata tggtttaatg actccaaaca
     1981 atgaaccttg tggaggtcaa aacataaatt atcctgcaga aggaacctta ataataggac
     2041 atcaaataga cgggttaata gatagtgttc acgaattaaa tattgaaaaa tcaactggaa
     2101 atggattccc ttttaaaaat aataacaaag aaggattaga atacggaata gtaaagatga
     2161 aagaatttgc agcattagaa gataaattaa gatataacca attaataaga atagcagaag
     2221 aaaccttaaa aaataattca tcaacagcaa gggcaaaaca attaattgaa gaagtatttc
     2281 ttccattata tgaagaaaaa atataatcta gattatttgt tatattaagt attttacaat
     2341 atgacttcaa attcacctag ctctttcttt aaaacatcaa aaacctttga gttaaacccg
     2401 acatctttct tattcttttg gtcttccctt gattttgaaa cttcttccat atttgaatct
     2461 tctaatttag aagttatctt gaatacttta ttctgtctaa acaagaaagc attctctcct
     2521 gaaagaagca actctccact tccttgttta aatttaatct tcattcttct tttttcaagt
     2581 aaagccatat cacgtctttg agaaaattca attaaatcat tgattaatgt tgaagcttct
     2641 tttcttgcat tatcgacttt atgagcatct aattttcctt tcttgaattc tgttctagca
     2701 gctataatat tttttaaaat tctcaagtgt tgttgagtca tttttccttt tttaacaaga
     2761 tttttttcaa actctaaaac taattgctgt tgagattttt tcccaaatat agcacttaaa
     2821 agattaattg tatcagaata aattttttca attatttttt cctgagcttt tttatcaatc
     2881 tgtgttctta attcttttaa cctcttcaaa aatccttcag tttctttaag aagtttatca
     2941 atctcagttc cagaaatttc ttttatcttt tcatgttcat aatctctcca gatattaatg
     3001 actttttcca taaaatcaat atatttcttt tcaatcattt ttgtatctaa gaattctctt
     3061 ttgaaatctc ttacaagttc tttctttgca tttggtggag gacacccatt taacattaat
     3121 aaagcttgag ctggagttgt aattccccag aagatattca caaaaatatc agacaataaa
     3181 gttcttttac ttcttggtat tactccgtct cccatgctca taaacatatc aatagcttca
     3241 ggagaaggtt tcaatttacc catcttaaga agagctttcc aaggcatgaa tgttcccctg
     3301 tcataaattg gaacaccatc tcttatgaat gtaaacataa caggatgagc atctttaaca
     3361 gcttcccaaa aatcagtaag aagatatatc tgaggttcaa gtttattatt aactcctgca
     3421 agagcacttg cttccccaac atactgatag attatacctc ttaatctttc tttaagttca
     3481 agtctaggca ttcttttaac atcagtatca ttaataataa caaaaacatc tacatcagaa
     3541 tcttttgatg cttctcctct tatcaagctt cctcctaaaa cataagaaac aacatatttc
     3601 tcaaatttct gtaacactaa cgatttatgt atttctgtta ctctcatagc acctaaaata
     3661 cctttatcgt gaagggggaa agacattgcc attgcagcat acaattcaaa tttttgatca
     3721 gtgcaaatct cccaaatttc agaaggagtt ctcatatata tccaaatttc ttgtttagat
     3781 ttttcaattt gttcaataat atctttcttt attttataaa tatcttttac tttctcatca
     3841 ggaattataa tatttaaatg aactttcttt tgaagttttt caatctgttc cttgtttaag
     3901 ttctctgttt cctcctcaat aaaatctttt attgattgtg gaggaagaat acttattgcc
     3961 tttgtaaatg gatatttttt aagaatatat cccttaagtt tatccagttc tttttttatt
     4021 ttatctccac tcttatcttt ttcagatgta ggggcttctg gaacagcaaa cttcttagga
     4081 ctataatttt tatccaaatc catttcagga atattagcat cagtatttat gttactttga
     4141 gccatgataa caaaagatgt tttttacttt taaatgtttg gttataaaaa ttaacaatag
     4201 aagagactat aaagttgtat agtttatttt ttattgttga aatctattta acttataact
     4261 tattcaataa tttaaacttc tttttagtaa attcaattat cttttcagca tattctttgt
     4321 caaatctctt tccgtaataa agaattccat ttctaaaatg ccttaattta tccaataact
     4381 caatatcaga ttcattaaat ttcaattttc ttgaataaga aacctctgct tcatgtgctc
     4441 cttgtccaga actggaatat ccttcttcaa gcatttttgc tcttattaag aagattaata
     4501 tgttataaca atcttcaatg taatcatttg cactttcatc atccactcct aatttatcaa
     4561 tctttttttg caataatttg aattttctcc cagattcaag ataaatatta tttgctctgt
     4621 tcttgtctat gcttattttc ttaacaatgc cttcctttag aaactcttca aaaatcttta
     4681 ttacactcat aaatttacac ccccatgcaa tacaatccca ttaagtatat tattctttaa
     4741 agaaatatgt attgagttcc aggagttgta gaaattaata tttattttct tatttaacct
     4801 tttttcataa ggtgcaagat taattttctt ttcttttctt tcaataatcg caatatcaat
     4861 atcagaggtg ttcgtatctt cccctcttgc atagctacca aataagatta ttgtacttcc
     4921 tgcaagattc tctccaagat aatcggataa tccagataag taaatgtttt tcaggttttc
     4981 tgctctcttt aattcaataa ctaatggttt atctctgttt aaagagataa aattaattgt
     5041 ttttgtcttc tcaatcctag cgaaatttat atctaatagc ttcttcagac ttttagctat
     5101 tgcagttggg gaaaccttta atgattgtgc aatttctctt tggcttagtt tttctcctgc
     5161 ccttaagcac aaaaaagaga atatttcctg ttccagttgt gtaaatttta gtttatatgt
     5221 atccattata gttcatatgt ataaactaaa gtttataaac cttttgtaga aataattaaa
     5281 ttgtatttgt cattgtttat tgttataatt ttttaacata tgttataata ttattacact
     5341 tttggataaa tgtttctgat ttggctttta tcaatagaat actataaaat tataaaatat
     5401 tgtacctcct acaagaacct ctcccaaata atcagataat ccaaataagt aaatattttt
     5461 caagttttct gttcttttta gttcaataac taattatttt ttgtaaaaaa tattttaaat
     5521 gtttggttat aaaaattaac aatagaagat actata
//
LOCUS       DAOPCH010000056         5480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_12757_length_5480_cov_3.827465, whole genome shotgun sequence.
ACCESSION   DAOPCH010000056 DAOPCH010000000
VERSION     DAOPCH010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12757_length_5480_cov_3.827465"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PLU55_04415"
     CDS             complement(<1..236)
                     /locus_tag="PLU55_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="binds directly to 16S rRNA where it nucleates
                     assembly of the head domain of the 30S subunit; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPJ87333.1"
                     /translation="MSEQTTNLKIFDRYDISTIQIQDEGLKSAINLKPKLVLKNQGRG
                     VGKFSQTKVNIVERLMNKISVAGHRGKKHRLEKG"
     gene            complement(229..495)
                     /locus_tag="PLU55_04420"
     CDS             complement(229..495)
                     /locus_tag="PLU55_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87334.1"
                     /translation="MAEQIKFHERYYEDVKRYLNSHKALHPGNSFGVDGKLYLTNGRK
                     KPSGFVLQRCCEEPSEIMLSVINYHPSFNQIKSDLQKIIESKNE"
     gene            complement(497..925)
                     /locus_tag="PLU55_04425"
     CDS             complement(497..925)
                     /locus_tag="PLU55_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPJ87335.1"
                     /translation="MQMGLKSAIKKKKDRKKFQWKDRKFKVRTLNLYAKSDPLEGANQ
                     AKGIVLSKVQKEAKQPNSAMRKCCKVQLTKNGRTVTAFIPGNLAQKFIDEHDEVMIER
                     IGGKQGRSKGDIPGVRYAVIKVNDQPLERLWLGKIQKGRR"
     gene            complement(979..1299)
                     /locus_tag="PLU55_04430"
     CDS             complement(979..1299)
                     /locus_tag="PLU55_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87336.1"
                     /translation="MEYNIDDKNYRIEAKGSEKEIKKFARCLVETINKHYLGEFDLYK
                     SRVASEPVQYNPTAYERIYLSNNSLLYLPNINEENGKKFHFNILMIHYKKAREKFEKI
                     SESF"
     gene            complement(1321..1737)
                     /locus_tag="PLU55_04435"
     CDS             complement(1321..1737)
                     /locus_tag="PLU55_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87337.1"
                     /translation="MVNTLDMKDLRYLNLFERVTGIRTHYCFEYNNTIIFCVPKELVS
                     KAIGPEVRNLRRISEIIKKRVKVIAIPSSSQDIKKFIELVVSPVTFKDLEISENEIVL
                     NAGAQSKAALIGREKRRLIEMQKITKDFFKKDFRII"
     gene            complement(1785..5276)
                     /locus_tag="PLU55_04440"
     CDS             complement(1785..5276)
                     /locus_tag="PLU55_04440"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006336.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HPJ87338.1"
                     /translation="MAKIIRKKIGEMKFSLFSPEQIKKLSCAKIVTPELYDIDGYPVD
                     GGLMDLRLGAVDPGVRCRTCGGRIKECLGHPGSIDLARPLIHLKYVPLIEMGLRCFCN
                     DCGKLMLSEKDLEKYTPSQRAKKAKDAKKCPYCQAIQERVRLDKPTTFYRGKVRIFPT
                     EIRELLVKIPDEELRKIGVNPNACRPEWAVLTSLLVPPVTVRPSIILETGERSEDDLT
                     HKLSDIIRANQRLWENLNAGAPEVIIEDLWDLLQFHITTFFDNTVTRIPPARHRSGQP
                     LKTITERIKGKEGRIRKNIAGKRVNYSGRTVISPDSSIDINEVGIPFEIARIVTVAET
                     VNDLNLERLKKLIQRGEEYPGANYVIRPDGKRKKISIDLKEEIINELSAGYQVERHLQ
                     DGDVVLFNRHPSLHRGSLMAHFVRILPGRTFRLHPAAAAPYNADFDGDEMNIHSPQNE
                     EARAEAKILLDVKRNLISPKNNTSLIGCIGDAITGNYLMGLEEFPVEYANQLLFNSEV
                     DAQFTKKTVSGTEIFSKILPKFNFSNESMIIKDGEIVKGVIDKTAFGSEDGELVKVLD
                     KTIGREETFKTIKRAFNLGKNYLSGRGITIAVNDLDLNEDILKHCEEVIEKAQKKTQE
                     IIDSDKDGTLELIPGKTKEESREIRILQTLNEVRTKIGEIVKREFPSENPVSSMIKSG
                     GGGNILNITQMASCVGQQALGSKRVDFGYNDRTLSFFKKGDLSPKARGFIKSSFIRGL
                     KPDEFFFQAITGRDSLMDTGLRTPKSGYLYRRLASALQDLRVEYDETVRDSNNNIIEF
                     KYGDDGIDVTKLHLKEKLDSGEAIGIVTAQSFGEGSTQMVLRTFHMAGVSEMQVTTGL
                     PRLIEIFDARKKPSSPKMEIYLNKEFNNEDGARFVAEKIKEVKLKEIASEINVDFSAR
                     KIEIKVDKEGLKQAHSTVKKVVEKLNELGFKVKEKQDYIYLDADDKIGFKDLYKLKEK
                     LKETIISGVKGVEQILVVKRDNNFVITTLGTNLKEIIAMKEVNSYKTISNDFYEVAEI
                     FGIEVARELLMHEITEVLKSQGLDIDIRHLKLVTDAMTNSGDIKGVTRMGIIAQKSSI
                     LARATFETPVPQFVKATVKGGNDKLLSVIENIILNQPVPVGTGLPGLLVKVIGPLTKK
                     ESEKKTADSKIVNKVNK"
     gene            complement(5278..>5480)
                     /locus_tag="PLU55_04445"
     CDS             complement(5278..>5480)
                     /locus_tag="PLU55_04445"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF016447.2"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. The beta
                     subunit is part of the catalytic core which binds with a
                     sigma factor to produce the holoenzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HPJ87339.1"
                     /translation="DKVVLPICTKCGTIAIEDSVRQKTYCPMCSSEDIELVEVSYAFK
                     LLLGELQGLHINTTFELKNKYE"
BASE COUNT         1603 a         1067 c          626 g         2184 t
ORIGIN      
        1 tttccctttt caagtctatg tttcttacct ctatgtcctg caactgaaat tttattcatt
       61 aatctctcaa caatattcac ttttgtttga ctgaattttc caacacctct accttgattt
      121 ttaagaacta atttaggttt caaattaata gcagatttta atccttcatc ctgtatctgg
      181 attgtagaaa tgtcatacct atcaaatatc tttaaattgg ttgtttgctc actcattctt
      241 actttctata atcttttgaa gatctgattt aatttggttg aaactaggat gataatttat
      301 cacagataac attatttcac ttggttcttc acaacatctt tgaagaacaa atccactagg
      361 tttttttcta ccgttagtca aataaagttt tccatctact ccaaatgaat taccaggatg
      421 taacgcctta tgactattca gatatctctt aacatcttca taatatctct cgtggaattt
      481 tatttgttca gccattttat cttctaccct tctgtatctt tccaagccat aatctctcaa
      541 gaggttggtc atttacttta ataacagcat atctaactcc aggaatatct cctttacttc
      601 ttccttgttt tccaccaatt ctttcaatca taacttcatc gtgttcatct ataaacttct
      661 gagctaaatt acctggaata aatgcagtaa cggttcttcc attctttgta agctggactt
      721 tgcagcactt tctcattgct gaatttggct gtttagcttc tttttgaact ttagaaagaa
      781 caattccttt agcctgattt gcaccctcta aaggatcaga tttagcataa agatttagag
      841 ttctaacttt aaattttcta tctttccatt ggaatttctt cctatctttc ttttttttaa
      901 ttgcactttt taatcccatt tgaattatta attatctatt atcaggaaat tagtttaaaa
      961 accttaccta tcttgttttt aaaagctctc actaattttt tcaaatttct ctctggcttt
     1021 tttgtaatgt atcattaaaa tattaaaatg aaatttctta ccattttctt cattaatatt
     1081 tggaagatat aataatgaat tattacttaa ataaattctt tcataagcag ttggattgta
     1141 ttgcactggt tctgaagcga ctctagattt atataaatca aattcgccaa gataatgttt
     1201 atttatggtc tccacaaggc atcttgcaaa tttctttatc tctttctctg aaccttttgc
     1261 ttctattctg taatttttat catctatatt atattccatt gtgtaaaaat aaaatattat
     1321 ttaaattatc ctgaaatcct ttttaaaaaa atcttttgtt atcttctgca tttcaattaa
     1381 tcttcttttt tcacgaccaa ttaatgcagc cttactttgt gctccagcat ttagaacaat
     1441 ttcattctca gaaatttcta aatccttaaa tgtaactgga ctcacaacta actcaataaa
     1501 tttttttata tcttgagaac tactaggtat tgcaattact ttcactctct tttttattat
     1561 ctcgcttatt ctcctaagat ttctaacttc tggaccaatt gcctttgaaa caagttcttt
     1621 tggtacacag aatataattg tattattata ttcaaaacaa tagtgtgttc ttatacctgt
     1681 gactctttca aaaagattta gataccttaa gtctttcata tcgagtgtat taaccatttt
     1741 tattaaataa gaaatgcttt tataaatctg tcgatgacaa ttaatcactt atttacttta
     1801 ttgacaatct ttgaatctgc tgtctttttt tcagattctt tttttgtcaa aggtccaatt
     1861 actttaacaa gtaatccagg taatcctgtt ccaaccggta ctggttgatt taatataata
     1921 ttctctatta cactcaataa tttatcattt ccacctttta ctgtagcctt aacaaattga
     1981 ggtacaggtg tttcaaatgt agctcttgca agtatagatg acttctgtgc aataattccc
     2041 attcttgtaa ctcctttaat atcacctgaa ttggtcatag cgtcggtaac aagtttcaaa
     2101 tgtcttatat caatatctaa tccctgtgac ttaagaactt cagttatctc gtgcattagt
     2161 aattctcttg caacctctat tccaaatatc tcagcaactt catagaaatc atttgaaatt
     2221 gttttataag aattaacttc tttcatagct ataatctctt taagatttgt tccaagagtt
     2281 gttataacaa aattgttatc tctctttaca accaatatct gttcaactcc tttaactcct
     2341 gaaattattg tttctttgag tttttctttt aacttataca aatctttaaa cccaatttta
     2401 tcatcagcat ctaaatagat ataatcttgc ttttccttaa ccttaaatcc tagttcattt
     2461 aatttctcaa ctactttctt aactgtagaa tgtgcttgtt ttaatccttc tttatctact
     2521 tttatttcta tcttccttgc actaaaatca acattaattt ctgatgcaat ttctttaagc
     2581 ttgacttctt ttattttctc agcaacaaat cttgcaccat cttcattgtt gaattcttta
     2641 ttgagataaa tttccatctt aggagaagaa ggtttctttc ttgcatcgaa tatttcaatt
     2701 aatcttggta aaccagttgt aacctgcatt tcagatactc ctgccatgtg gaatgttctc
     2761 aataccatct gtgttgaacc ttcaccaaaa gattgggcag taacaatacc tattgcttca
     2821 ccagaatcaa gtttttcctt aagatgtaat ttagttacat caattccatc atcaccatat
     2881 ttgaattcaa ttatattatt attgctatcc ctcactgtct catcatactc tactcttaaa
     2941 tcttgaagag cactagcaag tcttctatat aaataacctg atttaggagt tcttaaacca
     3001 gtatccataa gagaatctct tcctgtaatt gcctggaaga aaaattcatc tggttttaat
     3061 ccccttataa aagaactctt aatgaatcct cttgccttag gagataaatc tcctttcttg
     3121 aaaaaagata atgtcctatc attataacca aagtcaactc ttttgctacc aagagcctgc
     3181 tgtccaacac aagatgccat ctgagttata ttaagtatgt ttcctccacc acctgattta
     3241 atcatgctac taacaggatt ttcagagggg aattctctct ttacaatttc acctattttt
     3301 gttcttactt cattaagagt ttgaagtatt ctaatttccc ttgattcttc ctttgtttta
     3361 ccaggaatta attcaagagt tccgtcttta tctgaatcaa ttatttcctg agttttcttc
     3421 tgagcttttt caataacttc ctcgcaatgt ttaagaatat cttcatttaa atccaaatca
     3481 ttaactgcta ttgttattcc tcttccactt aaataatttt ttcctaaatt aaatgctctc
     3541 tttattgtct tgaatgtttc ttctcttcct attgttttat caagaacttt aactaattct
     3601 ccatcctcgc ttccaaatgc ggtcttatct ataactcctt ttacaatttc tccgtctttt
     3661 ataatcatag attcatttga aaaattaaac ttaggaagta tctttgagaa aatttctgtt
     3721 ccagaaacag ttttctttgt gaattgtgca tctacttctg aattaaaaag taattgattt
     3781 gcatactcta ctggaaattc ttctaatccc attaaataat ttccagttat tgcatcccca
     3841 atacatccaa ttaaactagt attgttctta ggagaaatta aatttctttt aacatctaaa
     3901 agtatttttg cttcagctct agcttcttca ttctggggag agtgtatgtt catctcatca
     3961 ccatcaaaat cagcattata tggagctgca gcagcaggat gtaatctaaa tgttcttcct
     4021 ggaagaattc ttacaaagtg tgccattaag cttcctctgt gaagacttgg atgtctgtta
     4081 aataaaacca catctccgtc ttgcaaatgc ctctccactt gataacctgc agacaactca
     4141 ttgattattt cttcttttaa atcaatagaa atcttctttc tttttccatc tggtcttata
     4201 acataatttg caccaggata ttcttctcct ctctgaatta atttttttag tctttccaaa
     4261 ttcaaatcat taacagtttc tgcaacagtt acaattcttg ctatctcaaa aggaatacct
     4321 acttcattta tatcaattga tgaatcagga gaaataacag ttctaccaga ataatttact
     4381 ctcttacctg caatgttttt tcttattctt ccttctttac ctttaattct ttctgtgata
     4441 gtctttaaag gttggccact tctgtgtctt gcaggaggaa ttcttgtaac agtattatca
     4501 aagaaagttg taatatgaaa ttgtaataaa tcccataaat cttctataat aacttcaggg
     4561 gcaccagcat taagattttc ccataatctt tgattggccc taattatatc tgaaagtttg
     4621 tgtgtcaaat catcttcact tctctcacct gtttcaagaa taattgaagg tctaacagta
     4681 actggtggaa ctaaaagtga agttaaaaca gcccattcag gtctgcatgc atttggattt
     4741 actcctattt ttcttaattc ttcatcaggt attttaacaa gcagttccct tatttcagtt
     4801 gggaatattc ttacttttcc tctataaaat gttgttggct tatcaagcct aactctttct
     4861 tgaattgctt gacaataagg gcattttttt gcatctttag cttttttagc tctctgactt
     4921 ggagtgtatt tttctaaatc cttttcactt aacattaatt ttccacaatc gttacagaaa
     4981 catcttaaac ccatttcaat taaaggaaca tatttcaaat gaatcaatgg tctagccaaa
     5041 tcaatacttc ccgggtgtcc taaacattct tttattcttc caccacaagt cctacatctt
     5101 actccaggat caacagcacc caatcttaaa tccattaatc caccatcaac aggatatcca
     5161 tcaatatcat acaattcagg cgtaactatt ttagcacatg ataatttttt aatttgttca
     5221 ggactaaata aactgaactt catttctcct attttctttc ttattatttt tgccatttta
     5281 ttcgtattta ttctttaatt caaaagttgt atttatatga aggccttgga gttctccaag
     5341 aagcaattta aatgcatatg atacttcaac tagttcaata tcctcgctgc tacacattgg
     5401 acaataagtt ttttgtctca cactatcttc aatagcaatt gttccacact tagtacaaat
     5461 tggtaaaaca actttatctg
//
LOCUS       DAOPCH010000057         5442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_12845_length_5442_cov_3.387971, whole genome shotgun sequence.
ACCESSION   DAOPCH010000057 DAOPCH010000000
VERSION     DAOPCH010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12845_length_5442_cov_3.387971"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5442
                     /locus_tag="PLU55_04450"
     CDS             <1..>5442
                     /locus_tag="PLU55_04450"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HPJ87340.1"
                     /translation="YYDNPSSSSGQNSINVWDNNYLTVQHLEEAGTGTRYDSKYRRNF
                     TTSGYDNDEKTIGKIDGADLFDGNNDALNSTENFLSNLGSFTIEGWIKPRAWGSRVSL
                     IGQNDVVEFFMDGSNTIMIWTDRGGSISVAYPYSLDTWHYITAVGTGSNLLLYFDGVQ
                     VASGGTSTNNYGVSTYPVKIGEGVVDATGGFFNGSMDEIRISNISRSANWINQSYQIV
                     NNQDNLVSFGIKEEKTKGIISTVIGATPFYTTTPNPLNSTTFSCLDNMKSSGGSCEVS
                     WNVNATGELNSIWEFFVLANNLNHQTYHNASSESQRIDITIASQVPPSVPQLYRPLNA
                     TAYSSIPRLNWTNSTDQNGDNIYYILQVSNVSDFSSLVFANYNIPETSSPTGITPTGI
                     TQEGTYYWRVLATDLIGNSSWSETRLFYYDLSAPEITFINQTGEDNRRINNTNWLNRG
                     EILSIFVNVSDINTDKVWAVVWQSVVGGIEKVKVFFTNLGNFLWKAEIPTNQSWGGFY
                     NYTIYANDTLGSQRNYSSNFTVLGGNATINISKIYVEPPANISVYGNITLSNSTLLSN
                     YPINLWLDGQLLFLQNLTPEGSYDFYKEFIETSNNEFSKGTFYQVEIENNQNLTLSDG
                     ATYGNFTKILDAGAMVSWNNLYWNFQGASCSGISSYQNGDSNSYIGGEDSYISSGHTT
                     VNYGSETQIIIDGSPTSERGLILFKDIIGRGFNQVPENSIIKNANLTFYVSDTGDQVD
                     VYQILENWTENQVTYNNRLSGTPWSSTGCSGSPSRSIIISDSFTASSIGKYTINVTNS
                     LKDWVSGSGNYGWIFNMPTSNGINILSNEHSVISERPILTVDYQSSECTNIRVYIRTS
                     NDKLTWTKWKEMSNGGTINDSNIYSRYLEYRIEFTSTTPSLSPVLKDLTVNYTGLTTD
                     SNGNYRYNFTSPSEFGNHNISVNTSFNTILIENSSQFFVQAGIPPNVFLISPSNNQWF
                     SQGEINLIYNVSDLNNDIIFSELIINGLSNLTNSSPIINNAYNNFTINFTSGEYNWTV
                     NVTDKSGYKATDSQRRFYIDLINPNISLIYPEEGDSFEASELNLSFNATDNMSPNLTC
                     SVVLDGNTIHSGINVINHAITNVSSGLLTGGIHYWNVTCTDNALRYSTSPTFSFNISD
                     TPPNVTLVYPNENHLDNDGVISFIYNVTDNTGLTGCYLYLNGVINQSNSSPILNYQNN
                     AFDVEGISEGNYNWTVECFDLSMSSSKPLPRNFSVDLYKPSIVLQAPSNSGTSLFSDV
                     LFNFTVTDTFDSSLNCNLTINGVVMDMFVSNSGNLTSRLISNLTDGLKYWNVTCVDDT
                     LNSNTSSTWTVNITEYPSVILNTANNSRFNQSLINLSYTPSDNTNLSSCSLYLNGALN
                     QTNSTPVLNNKINYFLINNPIEGVYSWHVSCVDYIGLVNSSETRIFYVDRTAPQINVI
                     YPRGEDVYASNITFNFTTIDYTDSNIRCNLTVNSSVMDINFSASNGTITSRTISGIGD
                     GYNLWNLTCWDSAGNINTSETFDFIRYTNPTVTLISPENNTWFNSGSFVLEYLPEDDS
                     GIVNASLYINGVYNRSNSSPVINRAYNTFSINGFSDGIYFWNINVTDPTGLTGIDEPR
                     KLYVDSSPPQITLNSPAQSSIISTNNVTFNFSVWDNLDLYPTCNFTLDGELEFSGDYL
                     NNSNILRYSVIRDGNHSWKIDCVDNASNINSSSTVYFTVEAPPIVELVSPVNNFFTRN
                     STMTFSYIPYDLIDIPVCNFYLDGVYNSSNNLIAENVLNNFTIQGISEGVHNWTVNCS
                     DSDDNWNWSLERIFYRDISPPSIILNSPEN"
BASE COUNT         1927 a          779 c          863 g         1873 t
ORIGIN      
        1 tattatgaca atccatcttc ttcttcagga caaaattcaa ttaatgtatg ggataataat
       61 tatttgactg ttcaacattt agaagaagca ggaacaggaa caagatatga tagtaaatac
      121 agaagaaact ttaccacaag cggatatgat aatgatgaaa aaactatagg taaaatagat
      181 ggagcagatt tgtttgatgg taataatgat gctcttaatt caacagaaaa ttttttgagt
      241 aatttaggct catttactat tgaaggttgg attaaacctc gtgcatgggg gtcaagagtc
      301 agtttaatag gacagaacga tgtagtggaa ttttttatgg atggttcaaa tacaataatg
      361 atttggactg atagaggagg aagcatatct gttgcttatc cttatagttt agatacctgg
      421 cattatatta ctgcagttgg aacaggttca aatcttttac tttattttga tggagttcag
      481 gtagcttctg gaggaacatc aactaataat tatggtgttt caacttatcc tgtaaaaata
      541 ggtgaaggag tagtggacgc tacaggcgga tttttcaatg gttctatgga tgaaattcgt
      601 atctctaata tttcgagaag cgctaattgg ataaatcaat cttatcaaat agtaaacaat
      661 caggataatc ttgtttcatt tggaattaaa gaagaaaaaa caaagggaat tatctctaca
      721 gtaattggag caactccttt ttacacaaca actccaaatc ctcttaattc cacaactttt
      781 agttgcttag ataatatgaa atcttcagga ggttcttgtg aggtgagttg gaatgttaat
      841 gcaacaggag aattaaattc aatttgggag ttttttgtat tagcaaacaa cctaaatcac
      901 cagacatacc ataacgctag ttcagaatct caaagaatag atattacaat tgcaagtcag
      961 gttcctcctt ctgttcctca actctatcgt ccattaaacg caacagctta ttcgtctata
     1021 ccccgtttaa attggacaaa ttcaacagat caaaatggag ataatattta ttacatttta
     1081 caagtaagca atgtatctga tttttcttca ttggtttttg caaattacaa tattccagaa
     1141 acctcttcac caacaggaat aactccaaca ggaataactc aagaaggaac ttattattgg
     1201 agagttcttg caacagattt aattggaaat tcttcttgga gtgaaacaag attattttat
     1261 tatgatttat ctgccccaga aattacattt attaatcaaa caggagaaga taatagaaga
     1321 ataaacaata ctaactggtt aaacagagga gaaatcctat caatttttgt taatgtctct
     1381 gatataaata cagataaagt ttgggcagta gtctggcaat cagttgttgg aggaatagaa
     1441 aaagtaaaag tgttttttac caacctagga aattttttat ggaaagcaga gattcctaca
     1501 aatcaatctt ggggaggatt ttataattac actatctatg caaatgacac attaggttct
     1561 caaagaaatt attcttctaa tttcactgtt ttgggaggta atgctacaat aaacattagc
     1621 aagatttatg ttgaacctcc agcaaatata tcagtttatg gaaatattac tttgtctaat
     1681 tcaactcttc tttcaaatta tccaataaat ctttggttag atggacagct attattttta
     1741 caaaatttaa ctcctgaagg aagttatgat ttttataaag agtttataga aacatctaat
     1801 aatgaatttt ctaaaggaac attttatcaa gtagaaattg aaaataatca aaatctaact
     1861 ttatctgatg gagcaactta tggaaatttc acaaagattt tagatgctgg agcaatggtt
     1921 tcttggaata atttatattg gaatttccaa ggagcttctt gttcaggaat atcttcttat
     1981 caaaatggag attccaactc atatattgga ggagaagata gctatattag tagtggacac
     2041 acaacagtca attatgggtc agaaactcaa ataataattg atggttctcc aacatcagaa
     2101 agagggttaa tcttatttaa agacattata ggaagaggat tcaatcaagt tccagagaat
     2161 tcaataataa agaacgcaaa tctaactttt tatgtttcag atacaggaga tcaggtagat
     2221 gtttatcaaa tcttagaaaa ttggacagaa aatcaagtaa catataataa cagattatct
     2281 ggaactcctt ggagttctac agggtgtagt ggcagtcctt cacgttctat aattatatct
     2341 gattccttta cagcttcttc aattggaaaa tatacaataa atgttacaaa ctctttgaaa
     2401 gattgggttt ctggttctgg aaattacggg tggatattta acatgcctac aagtaatggg
     2461 attaatatcc taagcaacga acatagtgta atttcagaaa gacctatttt aacagtagat
     2521 tatcaatcaa gtgaatgtac aaatattaga gtttacataa gaacatctaa tgataaatta
     2581 acttggacta agtggaaaga aatgtctaat gggggaacaa ttaatgattc aaatatttat
     2641 agtagatatt tagagtatag aatagagttt acatcaacca ctccttcact atctccagta
     2701 ttaaaagatt tgacagttaa ttacactgga ttaactactg attcaaatgg aaattataga
     2761 tataatttca cttctccttc tgaatttggt aatcataata tttctgttaa taccagtttc
     2821 aacacaattc taattgagaa ttcatctcaa ttttttgttc aagcaggtat ccctccaaat
     2881 gtattcttaa tctctccttc gaacaatcaa tggttctcac agggagaaat aaatcttatt
     2941 tataatgttt ctgacttaaa taatgatata atcttttcag aattgattat aaacggttta
     3001 tctaatttaa caaattcatc ccctataatt aataatgctt ataataattt tacaataaat
     3061 tttacaagtg gagaatataa ttggacagtt aatgtaacag ataagtcggg atacaaagct
     3121 acagattctc aaagaagatt ttatattgat ttaattaatc caaacatatc tttaatttat
     3181 ccagaagaag gagattcttt tgaagccagt gaattaaatc taagcttcaa tgctacagat
     3241 aatatgagcc ctaatttaac ttgtagtgtt gttttagatg gaaacaccat tcactctggg
     3301 ataaacgtaa ttaatcacgc cataacaaat gtttcttcag gattattgac tggagggatt
     3361 cactattgga atgtcacatg tacagataat gctcttagat attctactag tccaactttt
     3421 agttttaaca tctctgatac tccccctaat gtaactttag tttatcctaa tgaaaatcat
     3481 ttagataatg atggagttat atctttcatt tataatgtaa cagataacac aggattaact
     3541 ggttgttatt tatatttaaa tggagtcata aatcaaagca atagttctcc tatattgaat
     3601 tatcagaata atgcatttga tgtggaagga ataagtgaag gaaattataa ttggacagtg
     3661 gaatgttttg atttaagtat gagctcaagt aaacctctac ctagaaattt cagtgtagat
     3721 ttatataaac cttcaatagt cttacaagca ccatctaatt caggaacttc tcttttctca
     3781 gatgttttat ttaacttcac agttaccgat acctttgatt cttcattaaa ttgtaatcta
     3841 actatcaatg gagttgtaat ggatatgttt gtatctaatt caggaaattt aacttcaaga
     3901 ttaatttcta atcttacaga tggtttaaaa tattggaatg ttacttgtgt agacgatacc
     3961 cttaattcaa atacaagctc aacttggaca gttaatataa cagaatatcc ttcagttatt
     4021 ttaaacactg caaacaattc tcgttttaat caatctttaa ttaatttatc ttatactccg
     4081 tcagataata ctaatttaag ttcttgtagc ctttacttaa atggagcatt aaatcaaaca
     4141 aattcaaccc ctgtcttaaa caataaaatt aattatttct tgattaataa tccaatagag
     4201 ggtgtttata gttggcatgt ttcttgtgta gattatattg gattagttaa ctcttctgag
     4261 accagaatat tttatgttga caggacagct ccccaaataa atgtaattta tccaagagga
     4321 gaggacgtat atgcttcaaa tattaccttt aactttacta ccatagatta tactgattca
     4381 aatatacgat gtaatctaac tgtaaattct tctgttatgg atattaattt ctcagcaagt
     4441 aatggaacta ttactagcag aacaatatct ggaataggag atggatataa tttatggaat
     4501 ttaacttgtt gggatagtgc aggaaatata aatacaagcg aaacctttga ttttataaga
     4561 tatactaatc ctactgtaac tttaatctct ccagaaaaca atacttggtt taattcagga
     4621 tcttttgttt tagaatattt gcctgaagat gatagtggaa ttgttaatgc ttcgttgtat
     4681 ataaatggtg tttataatag gagtaattca tctcctgtaa ttaatagagc atataacacc
     4741 ttttcaataa atggattttc agatggaata tatttttgga atataaatgt tacagaccca
     4801 acaggattaa ctggaattga tgaacctaga aaattatatg tagattctag tcctccacaa
     4861 ataactctta attctccagc ccagtcttct ataatatcta caaataatgt aacctttaat
     4921 ttttcagtat gggataattt agatttatat cccacttgta attttacatt agacggagaa
     4981 ttagaatttt ctggagatta cttaaataac tcaaatatct taagatattc tgttattaga
     5041 gatggaaatc attcttggaa aattgactgt gtagataatg cttcaaacat taattcaagt
     5101 agtacagttt attttacagt agaggctccc ccaatagtag aattggtttc gcctgtaaat
     5161 aatttcttta caagaaattc aacaatgact ttctcatata taccatatga cttaattgat
     5221 atccctgttt gtaactttta tttagatgga gtttacaatt caagtaataa tttaattgca
     5281 gagaatgtgc ttaacaattt cacaatacaa ggaatatcag aaggggttca taattggaca
     5341 gttaattgta gtgattcaga tgataattgg aattggtctt tagaaagaat tttttacaga
     5401 gatatttccc ctccttcaat aattttaaac tcccctgaga at
//
LOCUS       DAOPCH010000058         5441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_12847_length_5441_cov_4.607315, whole genome shotgun sequence.
ACCESSION   DAOPCH010000058 DAOPCH010000000
VERSION     DAOPCH010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12847_length_5441_cov_4.607315"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..462
                     /locus_tag="PLU55_04455"
     CDS             196..462
                     /locus_tag="PLU55_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87341.1"
                     /translation="MTYKIPNLQDVEREIKEFLVKKDRFYFRNIYPILSYSSKEGYYT
                     VKIVAQDKKIGVNLKDYFDRTFKSGFGKEELETLLEKNHVKLII"
     gene            525..2723
                     /locus_tag="PLU55_04460"
     CDS             525..2723
                     /locus_tag="PLU55_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048060960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HPJ87342.1"
                     /translation="MAEKETSLDKIRRLVSNQNNIRNVATSAHIHHGKTAFTDNLLAA
                     AGMMAAKNAGDLEEGMATWQHSDEQERLMTVDAANVSMVHNYQGEEYLVNLIDTPGHV
                     DFGGNVTRAMRAIDGTFVLVCAVEGIMPQTETVIRQALKERVKPVLFINKVDRLINEL
                     QVTPEQMQERFKKIIEKFNHLIDQIAEKEFASKFKVNVTDGSVAFGSARENWALSIPF
                     MMKKKISFKDIYKLYDGTLSHEERDKWVWEHAPLYEVILDMAIKHLPNPIEAQSYRIP
                     KIWRGDLESDFGKDLIKCDKDGELGFVITRIVIDPRSGKEISAGRLYSGTLKSGMDVY
                     ANNAKKPGKIQQVLVYNGIKPEQLESVPAGNVLAVSGLNLDVGETITTKEQTAFEEIK
                     HIFQPVITKSIEVIKTADLPKLIEILRKVSKEDPSIKVTINEETGESLISGMGELHLE
                     IIENRIKTEKGLDVKTSNPIVVYRESVGKSSGQAFEGKSPNKHNSFFIRVEPLDDELS
                     DLIDKGELPEGRMKKKNDVVVSTLSKIGWGSDEIRNVKDIYKGNMFFDETRGEVHIGE
                     VIEMILDAFEMVMDQGPLAREPCMRMKVTLEDIKLHEDAIHRGPAQVYPAVREAITEA
                     MKSANATLLEPLQIHLIEVPEMFMGAATKLVGGKRGQLLDMTQEAGQAQLKAKIPVAE
                     MIGWSSDLRSATEGRGTSSLVDQHFERIPVALQADVIKKIRDRKGLAENQ"
     gene            2765..3217
                     /locus_tag="PLU55_04465"
     CDS             2765..3217
                     /locus_tag="PLU55_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87343.1"
                     /translation="MEKITFKLPQIIFTESGLNRYNYLERIASNSNEPKVNLTFQEDS
                     GEDSRIIAIKYQVEGEEGNDVLYYSEGAHRFSIITANLRFPYIGRVLVRDRNNHKIGV
                     DFENFFKKDLRQRHINKINKRGNIIKNLSPEYVAKRYKDIFYRLNIQD"
     gene            3251..3574
                     /locus_tag="PLU55_04470"
     CDS             3251..3574
                     /locus_tag="PLU55_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87344.1"
                     /translation="MNWKKIPYYIPGVGLATHTVREYKLRKGKNPWYNLKEPRDRKAL
                     ANYLVQVGYLSFAILWKVYVGSYLGKGINTGDWHPFRFDSKNKIESNIEDKIKKSNSL
                     ESIFR"
     gene            3711..4994
                     /gene="tuf"
                     /locus_tag="PLU55_04475"
     CDS             3711..4994
                     /gene="tuf"
                     /locus_tag="PLU55_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013683741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor EF-1 subunit
                     alpha"
                     /protein_id="HPJ87345.1"
                     /translation="MAKEKPTMNVVFVGHVDAGKSTCVGRLMFDGGAVSEQEMVRLKT
                     EAQKHGKAGFEFAYIMDSIKEERERGVTIDLSYKKLMTQKYEITIIDAPGHRDFVKNM
                     ITGASQADAAFLVIAAPAGVQPQTTEHLWLLRTMGVKNLAIAINKMDAVEYKEDRFKT
                     VKEDVSKLLKQVGINPDNTPFLAVSGLQGDNIFKKTDKMPWYKGPSVVEQIDLFPAPE
                     LPTNLPMRMPVQDVYEITGIGTVPVGKVETGIMKIGQKVIVLPGRSGTGIPGEVKTIE
                     AHHETLPDAPAGMNVGVNIRGVGKKDIARGDVICEASAPIPIVEEFIAQIAVINHPTV
                     LAKGYAPVFHVHTAQVPCQFTELIEQIDPKTGQVIKQNPDFLKNGDVAKVRIRPQGNL
                     CLETAKENPFMGRFAVRDAGATVAAGMCLEIVKKK"
     gene            5184..5390
                     /locus_tag="PLU55_04480"
     CDS             5184..5390
                     /locus_tag="PLU55_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87346.1"
                     /translation="MKQKDDYLLMIEDYECLNNNSKFECRFCNGFGKYLNKYIVESTA
                     ECYKSRIMGEGELKGLSKKIFEII"
BASE COUNT         2109 a          617 c         1040 g         1675 t
ORIGIN      
        1 cttcgaacag aagtaatctg aagctcaagc tgattctgct cgttaagctt catattcata
       61 agtcctcact ttgttgcggt gttctcattc ttcgaacaga agtaatctga agctcaagct
      121 gattctgctc gttaagcttc atatgtctta ttattatagt aattattaaa aactataatt
      181 tatgtttgta taaatatgac ttataagata cctaatttac aagatgttga aagagaaata
      241 aaggaatttt tagttaagaa agatagattt tattttagaa atatttatcc tattttatct
      301 tattcttcaa aagaaggata ttacacagtt aaaattgttg ctcaagataa aaagattggg
      361 gttaatttga aagattattt tgataggaca tttaaatctg ggtttggaaa agaagaatta
      421 gaaactcttt tagaaaaaaa tcatgttaaa ttgattattt gattagaaaa tattgaaaag
      481 atttaaaaac tatttttatc aactttaata attagcaaca taaaatggca gaaaaagaaa
      541 caagtttaga taagattaga agattggttt ctaatcagaa taatataaga aatgtagcta
      601 caagtgcaca tattcaccat ggtaagacag cttttacaga taatttgctg gctgctgcag
      661 gtatgatggc tgcaaagaat gctggagatt tggaagaggg tatggcaaca tggcaacatt
      721 cagatgaaca agaaagattg atgactgttg atgctgcaaa tgtttctatg gttcacaatt
      781 atcaaggaga agaatatttg gttaatttga ttgatactcc tgggcacgtt gattttggtg
      841 gaaatgttac aagagctatg agagctattg atggtacatt tgttttagtg tgtgcagttg
      901 aaggaataat gccacaaaca gagacagtta taagacaggc tcttaaagaa agagttaaac
      961 ctgttctttt tattaataaa gttgacagat taatcaatga attacaagtg actcctgaac
     1021 aaatgcagga acgtttcaaa aaaataattg aaaaattcaa tcatttaatc gaccagattg
     1081 ctgaaaagga atttgcaagc aagtttaaag ttaatgttac tgacggtagt gttgcatttg
     1141 gttctgcaag agaaaactgg gctttgtcaa ttccatttat gatgaagaaa aaaataagtt
     1201 tcaaagatat ttacaaatta tatgatggaa ctttatctca tgaagagaga gacaaatggg
     1261 tatgggaaca tgcgccacta tatgaagtta ttttagatat ggcaattaaa catttaccta
     1321 atccaataga agctcaatct tacagaattc ctaaaatatg gagaggagat ttggaaagtg
     1381 attttgggaa agatttgatt aaatgtgata aagatggaga acttggattt gttataacaa
     1441 gaattgttat tgatcctcgt tcaggaaaag aaatttctgc tggtagatta tattctggta
     1501 cacttaagag tggaatggat gtatatgcaa ataatgcaaa aaagccaggt aaaattcaac
     1561 aggttttggt ttataatggt ataaaaccag aacaattaga gagcgttcct gcaggaaatg
     1621 ttcttgcagt aagtggatta aatcttgatg ttggtgaaac aatcacaacc aaagagcaaa
     1681 ctgcatttga agaaataaaa cacatattcc aacctgtaat tacaaagtca attgaagtta
     1741 taaaaacagc tgatttacct aaactaattg aaatcttaag aaaagtttca aaggaagacc
     1801 cttcaattaa agttactatt aatgaagaaa caggggaaag tttaatttct ggaatgggag
     1861 aattgcatct tgaaataatt gagaacagaa ttaaaacaga aaaaggactt gatgttaaaa
     1921 cttctaatcc aattgttgta tatagagaat ctgttggaaa gagttcagga caagcatttg
     1981 aaggaaaaag tccaaataag cataatagtt tctttatcag agttgaacct cttgatgatg
     2041 aattatctga tttaattgat aaaggagaac ttccagaagg aagaatgaag aaaaagaacg
     2101 atgttgttgt aagtactctt tcaaagattg gatggggaag tgatgaaata agaaatgtta
     2161 aagatatcta taaaggaaat atgtttttcg acgagacaag aggagaagtt catattggtg
     2221 aagttattga aatgatactg gatgcatttg aaatggttat ggatcaaggg cctttggcaa
     2281 gagaaccttg tatgagaatg aaagtaacac ttgaagatat taaacttcat gaagatgcta
     2341 ttcacagagg accagctcag gtttatcctg ctgtaagaga ggccataact gaagcaatga
     2401 aaagtgcaaa tgctactctt cttgaacctt tacaaattca tttaattgaa gttccagaaa
     2461 tgtttatggg tgctgctaca aaacttgttg gtggaaagag aggacaactt ttagatatga
     2521 ctcaggaagc tggacaagct caattaaagg caaagattcc agtagctgaa atgattggat
     2581 ggagttctga tttaaggagt gcaactgagg gaagaggaac atcttcgtta gttgaccagc
     2641 attttgaaag aatacctgtt gcattacaag cagatgttat aaagaagatt agagatagaa
     2701 aaggattagc tgagaatcag taaacagtaa attttttaaa tcatttattt cttgaaaaaa
     2761 tcatatggaa aaaattacat ttaaactacc ccaaattatc tttactgaaa gtggattgaa
     2821 cagatacaat tatcttgaaa gaattgcatc aaattcaaat gaacccaaag ttaatttaac
     2881 atttcaggaa gatagtggag aagatagcag aatcattgca ataaaatatc aagtagaagg
     2941 agaagaaggg aatgatgttt tatattattc tgaaggagca cacagatttt ctataataac
     3001 tgctaatttg agatttcctt atattgggag agttcttgtt cgcgatagaa ataatcacaa
     3061 gataggtgtt gattttgaaa acttttttaa aaaagattta agacaaaggc atattaataa
     3121 aataaataag agaggaaata ttataaaaaa tttgagtcct gaatatgttg caaaaaggta
     3181 taaggatatt ttttatagat tgaatattca agattaaatt tataaataat ttttttaata
     3241 tatattttat atgaattgga aaaaaattcc atattatatt cctggagttg gtttagctac
     3301 tcatactgtg agagaatata aattaagaaa agggaaaaat ccttggtata atcttaaaga
     3361 acctcgagat agaaaagctt tggctaatta tttagtacaa gtaggttatc tgagttttgc
     3421 aatactttgg aaagtttacg tcggttctta tttaggaaaa ggaattaata ctggagattg
     3481 gcatccattt agatttgatt ctaaaaataa aattgaatca aatatagaag ataaaattaa
     3541 gaaaagtaac agtttggaaa gtattttccg ttgataaatt agaatttcat aaattctata
     3601 aaataatata taagaaataa aatcaaaaag tttataaact aactttttta ttaactaatt
     3661 tatagttaaa gtgaaattaa tatttcactg aatctattta aggtaacaaa atggcaaaag
     3721 aaaaaccaac aatgaatgta gtgtttgtag ggcatgttga tgctggtaag tctacttgtg
     3781 taggtagatt aatgtttgat ggtggtgcag tatcagaaca agaaatggtg agattaaaaa
     3841 ctgaagctca aaaacatgga aaagctggat ttgaatttgc ttatataatg gatagtatta
     3901 aagaagaaag agaaagagga gttacaattg atttatctta caaaaaatta atgactcaaa
     3961 aatatgaaat cacaatcatt gatgcacctg gacacagaga ttttgttaaa aacatgatta
     4021 ctggtgcttc acaagcagat gctgcatttt tagttattgc tgctcctgct ggagttcaac
     4081 cacaaacaac agaacactta tggcttttaa gaacaatggg tgtaaagaat cttgctattg
     4141 ctataaataa aatggatgct gttgaatata aagaagatag attcaagaca gttaaagaag
     4201 atgtttctaa attattaaaa caagttggaa taaatccaga taacacacct tttttggctg
     4261 ttagtggatt acaaggagat aatattttca aaaaaactga taagatgcct tggtataaag
     4321 gaccaagtgt tgtggaacaa atagatttat tccctgctcc agaattacca acaaacttac
     4381 caatgagaat gccagttcaa gatgtatatg aaattacagg aattggaact gttccagttg
     4441 gaaaagttga aactggtata atgaaaattg gacagaaagt catagttctt ccaggaagat
     4501 ctggaactgg aattccaggt gaagtaaaga ctattgaagc tcatcatgaa actttgcctg
     4561 atgctcctgc tggtatgaat gtaggtgtta atattagagg agtaggaaag aaagatattg
     4621 caagaggaga tgttatttgt gaagcaagtg ctccaatacc aatagttgaa gaattcattg
     4681 ctcaaattgc tgtaataaat catccaactg tgttggctaa aggatatgct cctgtattcc
     4741 acgtacacac agctcaagta ccttgccaat ttacagaatt aatagaacaa attgatccta
     4801 agactggaca agttataaaa cagaatcctg atttcttgaa gaacggagat gttgctaaag
     4861 taagaataag accacaagga aatttatgtt tagaaactgc taaagaaaat ccatttatgg
     4921 gaagatttgc agttagagat gctggagcta cagtagctgc tggtatgtgt ttggaaattg
     4981 taaaaaagaa ataaataggt gatttttcta taaaataaac ttaaaaaatt caataaaatt
     5041 taatcaaata aattctaaaa aatttaaaat gatattcttt caaattttat tccagcagtt
     5101 ttacaatctt ttgagaagtt tatgaagtat tgaatcaatt gaaaaggata agatttataa
     5161 aacaagaata aagataattt atcatgaaac agaaagacga ttatttactg atgattgagg
     5221 attatgaatg tttaaacaat aattcaaaat ttgaatgcag attttgtaat ggttttggta
     5281 aatatttgaa taaatatatt gttgaaagca cagcagaatg ttataaatct agaataatgg
     5341 gggaaggtga attaaaaggg ttatctaaaa agatttttga gataatttaa gatgacagaa
     5401 gaaactaaag aatatgagtg ttctaaatac ccagaaaata t
//
LOCUS       DAOPCH010000059         4696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_15187_length_4696_cov_31.375135, whole genome shotgun
            sequence.
ACCESSION   DAOPCH010000059 DAOPCH010000000
VERSION     DAOPCH010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15187_length_4696_cov_31.375135"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PLU55_04485"
     CDS             complement(<1..749)
                     /locus_tag="PLU55_04485"
                     /inference="COORDINATES: protein motif:HMM:NF025655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4373 domain-containing protein"
                     /protein_id="HPJ87347.1"
                     /translation="MATKERLRKNDLDYFPLEININQDSKILLLEGKFGLEGFAVLIK
                     LYIKIYGDKGYYCEWNEVEKLLFARMLGIDADRLDEIIANCIKFKIFDKKMYEKYSIL
                     TSKGIQERFFFITSRRKKINVENSYLLINCEEYRAVEKTKKTVENSKMQIEDVNKQSE
                     LVKKQTKDAFLQQSKVKESKVNDIKIKDKGFLQNVENYHPLLELLAKNEYIDANTTTR
                     QLEQFNDLFFNLERDYELELIKAATSYITRFV"
     gene            complement(786..1469)
                     /locus_tag="PLU55_04490"
     CDS             complement(786..1469)
                     /locus_tag="PLU55_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPJ87348.1"
                     /translation="MEIKVIASSSSGNCYFVSDGITNLLIEAGVNLNRIRKYVNLSSL
                     SGCLISHEHLDHSKYAKEIAKYCNIYSSKGTLEALGSFGNFEFRKQAINGLIKIGTFY
                     IKTFETVHDAIDPVGFVILSSHLKETLFFATDTKVIKNKFENLDYIMIEANYSLNIAD
                     NNLTALERRRYNTHLSLEGVVAFLKTLDLTKTKKIYLMHLSDSASDEKYFKETVQKLT
                     GKEVIICEK"
     gene            complement(1482..2441)
                     /locus_tag="PLU55_04495"
     CDS             complement(1482..2441)
                     /locus_tag="PLU55_04495"
                     /inference="COORDINATES: protein motif:HMM:NF015776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HPJ87349.1"
                     /translation="MQEKQQDLQVKQDSKAVSKTKDLGKSMLDGMLKQVEAFAKLDQT
                     PLNQQETAFALDILESVYKKVMEQGLSWGQIDIMGCGLYSQVKSYARLGLTIRDNELY
                     IDIRNNGKTGLKDINIKKQYQGLEKELVKWCSKKIVRFYKDIVCAGDNLEKEFNFETG
                     LTEIKKHEKDPKVDRNKLDNIVGAYAIAYVRELPTDTKTTPYMIYIDRNRIMRAYNAS
                     PTKEKTIWNLDTRKMVLKTASWELYNYLKPFIEIPIELKKDWETTKDEMNWETGEVID
                     VVETETASDELISFDEEVEAEPTQAIAEEDIPVANPNYKKRPF"
     gene            complement(2457..4433)
                     /locus_tag="PLU55_04500"
     CDS             complement(2457..4433)
                     /locus_tag="PLU55_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87350.1"
                     /translation="MKEIKIIKLHIENFKGFEYKTFEFNGKSADIFGTNETGKTTIAD
                     AFYWCLFEKDSLGATDFAIKPKDKITGEDKHNLLTKVILTLLVNNQTLKLERTYEEVY
                     TRKRGSLEETFSGHTSNYFVNDIPKKKSEYNEVVRELLDEELFKTLTNVNYFNNLKWK
                     EQRDILVTLVKDFDFYSLLQDAKFIDLKEILLNDKTIKVDELIKSYKSKNTLLNKELT
                     AIPIQIQTLSNSLEDLNIMYSAVELEKIIKEKENELLSLNSKKQELDKGLIDYELENK
                     ISSLKAEVKANLIKIQEILKQVEQRLKSNLQDKEIKLLELNNAIARENMALKNFASNK
                     TLLENQREELYQRYDKVAESVFTGGTCSYCGQALPEEQTNELREKFNLNKSQELESIT
                     QKGLSINAELENIDTSYKAIFKAKEENQKEFNKLELEKKVIADLLNEFANRNYDKALT
                     EKETKDITALLDLNDNYNLEIDKLEHNKAATAVDTSERDKLNADIFALEKTIDSLKEQ
                     KTLIGVKNNTIEKINTLNDKIKTIQVEYEYNLSIITQAEEYSRLKAGVLEDSINSHFK
                     LIEFKLFKEQINGGIEETCIATVNGVPYTSINNAARINAGLDIINTLQEIYQVKAPIF
                     IDNAESVVEVLGMNSQLIKLYVSENDKTLRVELQ"
     gene            complement(4487..>4696)
                     /locus_tag="PLU55_04505"
     CDS             complement(4487..>4696)
                     /locus_tag="PLU55_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPJ87351.1"
                     /translation="DFRLIVITGKLVGYRKAANLTQEDMAKKLGITTATYNKKENNPN
                     LFTYAEQVKIEEVLRSYLKDMPAIF"
BASE COUNT         1361 a          823 c          558 g         1954 t
ORIGIN      
        1 acaaatcttg ttatgtaact tgttgctgct ttgataagtt ctaactcgta gtctctttca
       61 agattaaaga aaaggtcatt aaattgttcc aattgccgag tggtagtatt agcatcaatg
      121 tactcatttt ttgctaacaa ttctaacaaa gggtgatagt tttccacatt ttgcaaaaac
      181 cctttatctt ttatctttat atcatttact ttactttcct ttactttact ttgttgtaaa
      241 aaagcatctt tagtttgctt ttttacaagt tcactttgct tatttacatc ttcaatttgc
      301 atttttgagt tttccacagt tttttttgtc ttttccacag ctctatattc ttcgcaattt
      361 atgagtaaat atgagttttc cacattgatt tttttgcgcc ttgaagtgat aaaaaagaac
      421 ctttcttgta ttcctttgct cgttagaatt gagtattttt cgtacatttt cttatcaaag
      481 attttaaact ttatacagtt tgcaattatc tcatctaacc tatcagcatc tattccaagc
      541 attctcgcaa agagtaattt ctcaacctca ttccactcgc aataataacc tttgtcgcca
      601 tagattttta tgtaaagttt tatcaaaaca gcaaagccct caagtccaaa cttgccttct
      661 aaaagcaaga tcttactatc ttggttaatg tttatttcta aaggaaaata atctaaatca
      721 ttttttctta atctttcttt agttgccata attattcccc cttattttta agtgattagt
      781 tcctactatt tctcgcatat aataacctct tttccagtta gcttttgcac tgtttcttta
      841 aaatacttct catcacttgc actgtctgat agatgcatta agtatatttt ctttgttttc
      901 gttagatcta aagtttttaa gaaagctact accccctcta aagaaaggtg tgtattgtat
      961 cttcttcttt ctaaagctgt taaattattg tcagctatat taagagaata attagcttca
     1021 atcattatgt aatcaagatt ttcaaactta tttttaataa ctttagtatc agttgcaaag
     1081 aataatgttt cttttaaatg acttgacaag attacaaaac ctactggatc tattgcatca
     1141 tggactgtct caaaggtctt aatgtaaaat gttcctattt taataagtcc atttatagct
     1201 tgttttctaa actcaaaatt accaaaacta cctaatgcct ctaatgttcc ttttgaagaa
     1261 taaatgttgc agtatttagc aatttctttt gcatatttag agtgatctaa atgctcgtgt
     1321 gaaatcaagc atccacttaa agaagagagg ttaacatatt ttctaattct atttaagtta
     1381 accccagctt caattaatag gttagtaatt ccatcactta caaaataaca attaccacta
     1441 cttgatgatg caattacttt aatttccata actaacccct tttagaaagg tcttttttta
     1501 taatttggat tagcaactgg tatatcttct tcagctatag cttgtgtcgg ttcagcttca
     1561 acttcttcat caaaactaat tagttcatca cttgcagtct cagtttcaac tacatcaata
     1621 acctcgccag tttcccagtt catttcatct ttagtagttt cccaatcttt cttaagttca
     1681 attgggattt caataaatgg ttttaagtag ttataaagtt cccaacttgc agtctttaat
     1741 accattttgc gtgtgtctaa gttccagata gttttttctt ttgttggaga tgcgttataa
     1801 gccctcataa ttctatttct atcaatatag atcatataag gtgttgtttt agtgtcagtt
     1861 ggtagttctc ttacataagc aatagcataa gctcccacaa tgttatcaag tttatttcta
     1921 tcaactttag gatctttttc gtgtttctta atttcagtta acccagtttc aaaattaaac
     1981 tctttttcta aattatcgcc agcacaaaca atatctttat aaaatcttac tattttttta
     2041 ctgcaccatt taacaagctc tttctctaac ccttgatatt gttttttaat gttaatgtct
     2101 tttagtccag ttttgccatt atttctaata tcgatataaa gctcattatc tctaatagtt
     2161 aagcctaatc ttgcataact tttaacttgt gaataaagtc cacaacccat aatgtcaatt
     2221 tgcccccagc ttaaaccttg ctccattact ttcttataaa cactttctaa aatgtctaaa
     2281 gcaaatgctg tctcttgttg gtttagtggt gtttggtcta atttagcaaa tgcctctact
     2341 tgctttaaca taccatctag cattgacttt cctaaatctt tggttttact aaccgccttt
     2401 gagtcttgtt ttacttgtaa atcttgttgt ttttcttgca ttttaatttc ctccctttat
     2461 tgcaattcaa ctcttaaagt cttatcgttc tctgaaacat acagctttat tagctgacta
     2521 ttcattccta atacttctac aaccgactca gcattatcaa tgaaaatcgg tgcttttact
     2581 tgataaatct cttgtaaagt attgataata tcaagtcctg catttattct tgctgcattg
     2641 ttaatagatg tataaggcac accattaaca gtagcaatac atgtttcttc aataccacca
     2701 tttatttgtt ctttaaatag cttaaactca attaacttga aatgactatt gatgctatct
     2761 tctaacacac cagctttaag tcttgagtat tcttcagcct gagtaataat tgataggtta
     2821 tattcatatt ccacttgaat agttttaatc ttatcattta gagtgttaat cttttcaata
     2881 gtattattct taacaccaat taaagtcttt tgttctttta aactatcgat agttttttcc
     2941 aaagcaaaga tatcagcatt taatttatct ctttcacttg tatcaactgc agttgcagct
     3001 ttattatgtt ctaacttatc aatctctaaa ttgtaattat cgtttagatc taataaagct
     3061 gtaatatctt tagtttcttt ttcagttaaa gctttatcat aattcctatt agcaaattca
     3121 tttaatagat cagcaataac tttcttttca agttcaagct tattaaattc tttttggttt
     3181 tcttccttag ctttaaaaat tgctttatag cttgtatcaa tgttttcaag ttcagcgttt
     3241 attgataaac ctttttgagt aatggattct aactcttgag atttgtttaa attaaacttt
     3301 tctcttagtt catttgtttg ctcttctggt aaagcttggc cgcaatagct acaagttcca
     3361 ccagtgaata ctgactcagc aaccttatcg tatctttgat ataattcttc cctttggttt
     3421 tctaacaaag ttttattgct tgcaaagttc ttaagtgcca tattttctct tgcaatagca
     3481 ttattaagtt ctaataactt aatctcttta tcttgtaagt ttgattttaa tctttgttct
     3541 acttgcttta aaatctcttg tattttgatt aagttagctt taacttcagc tttaaggctt
     3601 gatattttgt tttcaagctc ataatcaatt aaacccttat ctaattcttg ttttttagaa
     3661 ttaaggctta aaagttcatt ttctttctct ttaataatct tttcaagttc tacagctgaa
     3721 tacatgatat ttagatcttc caaagaatta gataaagttt gtatttgaat tggtatagct
     3781 gttagttctt tgtttaataa agtgttctta ctcttatagc ttttaattag ttcatcaact
     3841 ttaattgttt tatcattaag taaaatctct tttaaatcaa taaactttgc atcttgaagc
     3901 agtgaataaa aatcaaaatc tttaactaaa gtaactaaaa tgtctctttg ttctttccac
     3961 tttaaattat taaaatagtt aacattagtt agtgttttaa ataattcttc atctaatagt
     4021 tctcttacta cttcgttata ctctgatttc tttttaggaa tatcattaac aaaatagttt
     4081 gaggtatgcc ccgagaatgt ttcttctaat gagcctcttt ttcttgtgta aacttcctca
     4141 taagttctct caagctttaa tgtttgatta tttacaagta aagttaaaat aactttagtt
     4201 agtaaattgt gcttgtcctc gccagtaatc ttatctttag gtttaattgc aaagtcagtt
     4261 gctcctaaag agtctttttc aaataaacac caataaaaag catctgctat tgttgttttc
     4321 ccagtttcat tagtgccgaa tatatcagca cttttgccat taaactcaaa agttttatat
     4381 tcaaaacctt taaagttctc aatgtgtaat ttgataattt taatttcttt catttctttt
     4441 cctttcttgt tttgtttctt tattataaac tataacacac aagccattaa aaaatagctg
     4501 gcatgtcttt caaataagac ctcaatactt cttctatttt tacttgctct gcgtaagtaa
     4561 atagatttgg attgttttct ttcttgttgt aggtagctgt tgtaatacct aacttttttg
     4621 ccatgtcctc ttgtgttaaa ttagcagctt tacgatagcc aactaatttc ccagtaatta
     4681 caattaatct aaaatc
//
LOCUS       DAOPCH010000060         4491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_16000_length_4491_cov_2.419748, whole genome shotgun sequence.
ACCESSION   DAOPCH010000060 DAOPCH010000000
VERSION     DAOPCH010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16000_length_4491_cov_2.419748"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..786)
                     /locus_tag="PLU55_04510"
     CDS             complement(16..786)
                     /locus_tag="PLU55_04510"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPJ87352.1"
                     /translation="MKTITIANQKGGVGKTTTAQNLAIGLAKKKFKVLAIDCDSQENL
                     SFSCGIVKPQKSVFNVLLQEPIEECIIHTEQGIDLIPSDERLLLADSTFIQENDIFRL
                     KVQLQRVSDKYDFVIIDTPPTLNTITKNCFIASDSIIIPMHTEIFSMQGLSKLINIIN
                     EAQNKQREKNLNEVKIEGILLTQFNGRTILNRALTGQIEKISKQTGLKVFKTTIRNGI
                     AIREAQNNQSNIFDYDSRSKVAKDYQDFVNEFLKVMKK"
     gene            complement(783..980)
                     /locus_tag="PLU55_04515"
     CDS             complement(783..980)
                     /locus_tag="PLU55_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87353.1"
                     /translation="MIKEGEIKKEYLQLLRKMKKQFKATDDCKEKSQFILAIDNYIKT
                     IKSDEISDNDLIEYLKREMMK"
     gene            complement(977..1213)
                     /locus_tag="PLU55_04520"
     CDS             complement(977..1213)
                     /locus_tag="PLU55_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87354.1"
                     /translation="MIATGNPSSRYEVATYYEDYSESTYFVRMNGAKKNFDEKKEDFK
                     HNKSIKKILFNEVILKNNEIITEPIEKIEREDSE"
     gene            complement(1210..1431)
                     /locus_tag="PLU55_04525"
     CDS             complement(1210..1431)
                     /locus_tag="PLU55_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPJ87355.1"
                     /translation="MNESKEKRGVGKPLIDLKERVESIRLEDDFYTIEELAKILKVHP
                     NTIRNNIKKGKINAKKYGEQWRIRKSDIL"
     gene            complement(1436..2842)
                     /locus_tag="PLU55_04530"
     CDS             complement(1436..2842)
                     /locus_tag="PLU55_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPJ87356.1"
                     /translation="MNELSIVNFHSINAIPQDRVIFKEDYFQDFLDFNKGRSTRTKEN
                     YLTCLKPFREWILNEQIINPTRKDCQRFRDYLDSLPLCEGTKQQYFRAMKHFFKWISS
                     EGIYPDIATNIQGFKVNLDISKKDSFKDSEVEQIIDSIDTTTEKGKRDKAIILLMITC
                     GLRINEARLIDIQDIEEREGKMKVYIQGKGHSEKDDYKFITPEVYQAIEEYLETRPNA
                     KKEEPLFTGTSNNQKGTRITETSFSRLVKDIFKKGGFNSKRLTAHSLRHTSSTILYES
                     GANLYQVQMHSRHKNPKTTEIYIHRVEMDKETSEQDIFNQIFNKNAIKNKREVIEEVN
                     SLSEEQLQKVALYIANLKEGGNTNEQFKENQIPQNAPMEDFISCGMGGYIHKSNKSKL
                     DDFFNQTEKELKKDFVSRITIESMIEYELINHECFYTGEPLSIVDLIQSYYSDKPREE
                     IVNLIKSIYHSKNKEMEA"
     gene            complement(2919..3326)
                     /locus_tag="PLU55_04535"
     CDS             complement(2919..3326)
                     /locus_tag="PLU55_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87357.1"
                     /translation="MTINNKYYKIKIDKDKKGDETMKKFLSVKWENVFIILTIPLQVI
                     QVLNAHQDFKLIAILTCLTLYEGLALAIHTGRKETLQEIKEGTFEPLIDFEEMFEELQ
                     GFIKVISQTKKKLFSKTKSINHNRQMNVFSNNF"
     gene            complement(4189..>4491)
                     /locus_tag="PLU55_04540"
     CDS             complement(4189..>4491)
                     /locus_tag="PLU55_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004836118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HPJ87358.1"
                     /translation="GITFDSRLEGKRYKELKLLERANLIEELTLQPSFELQPSFKKNG
                     KTIRAITYKADFSYIDKEKNCLIVEDTKGMKTRDYLIKKKLFEYKFPDLTIREITK"
BASE COUNT         1135 a          714 c          690 g         1952 t
ORIGIN      
        1 attgttttca tcattttatt tcttcattac ttttaaaaac tcattgacaa aatcttgata
       61 gtcttttgca actttgcttc ttgaatcata gtcaaagata tttgattgat tgttttgtgc
      121 ttctcgaata gcaattccat tcctgattgt tgttttgaaa actttcaatc ctgtttgttt
      181 tgatattttt tcgatttgtc ctgtcaatgc tcgattcaag attgttcttc cgttgaattg
      241 tgtcagaagt attccttcaa tcttcacttc attgaggttc ttttctcttt gtttgttttg
      301 tgcttcattt ataatattta tcaattttga caatccttgc attgaaaaaa tctctgtgtg
      361 cattggaatt ataattgaat cagaagcaat gaaacaattc tttgtgattg tgttcaaggt
      421 tggtggtgtg tctataataa caaaatcata tttgtcagac actctttgaa gttggacttt
      481 caatctgaaa atgtcatttt cctgaatgaa tgtagaatcc gcaagaagta gtctttcatc
      541 acttggaatg aggtcaatcc cttgttctgt gtgaatgata cattcttcaa ttggttcttg
      601 taataataca ttgaaaacag atttttgagg ttttacaatc ccacaactga atgaaagatt
      661 ttcttgtgaa tcacaatcaa ttgcaaggac tttgaatttc ttttttgcaa gtccgattgc
      721 aagattttgt gcggttgttg ttttaccgac tccgcctttt tgatttgcaa ttgtgattgt
      781 tttcatttca tcatctccct ttttaaatat tcaatcaggt cattgtctga tatttcatca
      841 ctcttgattg ttttgatata gttatcaatt gcaagaatga attgtgattt ttctttgcaa
      901 tcatctgttg ccttgaattg tttcttcatc tttcttaata gttgaagata ttcttttttg
      961 atttctcctt cttttatcat tcagaatcct ctctttctat cttttcaatt ggttctgtta
     1021 tgatttcatt attcttcaga atcacttcat tgaataagat tttcttgatt gatttattat
     1081 gtttgaaatc ttccttcttt tcatcaaagt ttttctttgc tccgttcatt ctcacaaaat
     1141 aggtgctttc tgaataatct tcgtaatatg tagcaacttc atatcttgaa gaaggatttc
     1201 ctgttgcaat cataaaatat cactctttct gattctccat tgttctccat attttttagc
     1261 attgattttg cccttcttga tattgtttcg gattgtgtta gggtggactt tcaaaatctt
     1321 tgctagttct tcgattgtgt agaaatcatc ttcaagtctt atgctttcaa ctctttcttt
     1381 taagtcaatc aaaggttttc cgactcctct tttctctttg ctttcattca ttcttttacg
     1441 cctccatttc tttatttttc gaatgataga ttgatttgat taagttcaca atttcttctc
     1501 ttggtttgtc tgaataataa ctttgaatca agtccacaat tgaaagtggt tctcctgtat
     1561 agaaacactc gtgattgatg agttcgtatt caatcattga ctcgattgtt attcttgaaa
     1621 caaaatcttt cttcagttcc ttttcagttt gattgaaaaa gtcatcaagt tttgatttgt
     1681 ttgatttgtg aatataacct cccattccgc aagaaataaa atcttccatt ggtgcatttt
     1741 gtgggatttg attttccttg aattgttcat ttgtgtttcc tccttctttt aaatttgcta
     1801 tatataacgc aactttttga agttgttctt ctgacaagga attgacttcc tcaatcactt
     1861 cccttttgtt ttttatagcg tttttattaa atatttgatt gaaaatgtct tgttctgaag
     1921 tttctttgtc catctctact ctgtgaatat agatttctgt tgttttaggg tttttatgtc
     1981 ttgagtgcat ttgcacttga taaagatttg ctcctgattc ataaagaatt gttgaagaag
     2041 tatgtctcaa ggaatgggcg gtcaatctct ttgagttgaa tcctcccttc ttgaatatat
     2101 ctttcacaag tcttgaaaaa gaagtttctg tgattcttgt tcccttttga ttgtttgaag
     2161 ttcctgtgaa tagtggttct tctttctttg cgtttggtct tgtttcaaga tattcctcaa
     2221 ttgcttgata gacttcaggt gtgatgaatt tgtagtcatc tttttcagaa tgtcctttgc
     2281 cttgaatata gactttcatt tttccttctc tttcttcaat atcttgaata tcaatcaatc
     2341 ttgcttcgtt gattctcaat ccgcaagtta tcatcagaag aatgattgct ttgtcccttt
     2401 ttcctttttc tgttgtagta tctattgaat caattatttg ttcaacttct gaatccttga
     2461 aggaatcctt ttttgaaata tcaagattga ctttgaatcc ctgaatgttt gttgcaatgt
     2521 cagggtatat tccttcagat gaaatccact tgaagaagtg tttcattgcc ctgaagtatt
     2581 gttgttttgt tccttcacaa agtggaagtg aatcaagata gtctctgaat ctctgacaat
     2641 cttttcttgt aggattgatg atttgttcat tcaaaatcca ttctctaaaa ggtttcaagc
     2701 aagtcagata gttttctttt gttcttgtgc ttctcccttt gttaaagtca aggaagtctt
     2761 gaaaatagtc ctccttgaaa ataactctgt cttgtgggat tgcattgatt gaatgaaagt
     2821 tcacaattga tagttcattc atttgaattg cctccttctt ttagggaaaa ctttgttccc
     2881 ttctcttgtc attcttgttg ttttatagta taattatttt agaaattatt tgaaaaaaca
     2941 ttcatttgtc tgttgtggtt gatagacttt gtttttgaga atagtttctt ttttgtttgt
     3001 gaaataactt tgatgaatcc ttgtagttct tcaaacattt cttcaaaatc aataagtggt
     3061 tcgaatgtgc cttccttgat ttcctgaagt gtttcttttc ttcctgtatg aattgcaagt
     3121 gctagtcctt catataatgt caaacaagtc agaattgcaa tcaatttgaa atcttggtgt
     3181 gcatttaaaa cttgaatcac ttgaagcggt atagtcaaga tgatgaatac attttcccat
     3241 ttgactgaca aaaacttttt cattgtttca tctcctttct tgtctttatc tatcttgatt
     3301 ttataatatt tgttattgat tgtcaagtgg ttatctcaaa aatctgataa aattgtgata
     3361 aaaggtgtaa acaaaaagaa agtcccttga atttcctgtt tttaggtcat ttgaaggact
     3421 ttttcttttg gaagtcttat tcatcaaggg aagtgctttt cgttcaatgt aggtgtgttt
     3481 ctgaaggtca tttttttatg aggtagaaaa ttgcttcaat ctgatgaata ttttgtcata
     3541 aaagtatcac aattcattgc actttttttg ctttattttg tccgaaaata gcactctcct
     3601 attaagagaa ttgactctcc tgaaagtaga atcccttgtt ttatgcctta tttatcaagg
     3661 aattgcaatt ctccttgtag gttgtattgt atgtcatttt attgcgtttt tatttccttt
     3721 tttgtatgca attatttgca tttttttgtc attattctgt catttttata tcagatattt
     3781 tgcacttttt ttttcactta tttatcagat ttatctcatt tatattttaa atatctgaaa
     3841 cactttgaaa catattgaaa caattttgct ttttttatcg tggggaaata ttctcttttt
     3901 aaggactcca aaatctccaa aatagaaaaa tattcatctt ctttttaggg gttcggaaaa
     3961 tccgcaatat tcttttttta tagtattgga tttcttggat tttagttttc tttttatagt
     4021 gtcggaataa tcaatttcac aatatgcctt cagaagcgtt ttaatgccct ttttagacac
     4081 tttttgattt tatagtatat ttattcattt tgattgataa agtgttggaa attgcctcct
     4141 attgtgtgaa ttttaagcaa taaaaaagga atcgtgattg attctctttt attttgttat
     4201 ttctcggatt gttaagtcag ggaatttgta ttcaaacaac ttcttcttga ttaagtagtc
     4261 ccttgtcttc attccctttg tatcttccac aatgagacaa ttcttttctt tgtctatgta
     4321 ggaaaaatcc gctttgtagg ttattgctcg aatagttttt ccattctttt tgaatgaggg
     4381 ttgcaattca aaggaaggtt gcaaggtcaa ttcttcaatc agatttgctc tttcaagaag
     4441 tttcagttcc ttgtatcttt tcccttcaag tcttgaatca aaagtgattc c
//
LOCUS       DAOPCH010000061         4258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_17045_length_4258_cov_38.516536, whole genome shotgun
            sequence.
ACCESSION   DAOPCH010000061 DAOPCH010000000
VERSION     DAOPCH010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17045_length_4258_cov_38.516536"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="PLU55_04545"
     CDS             <1..896
                     /locus_tag="PLU55_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPJ87359.1"
                     /translation="KKMSLTNKAGMPAILYEALCSLEANNEHLHNQGWSVTEILEPTR
                     IAVLKKKYAPKLEIDAIDQMASALGTAVHILLEQGHKGKEDLGIFVEKKVNLEINGVI
                     LSGKIDYYNQNDELIVDYKYQNVQSWVNIQDKLDYPTWQLNLYKYLLHKNNYPVKHLQ
                     VLFIFKDFREYEAGKFKSYPETLWKLINIESKPEEEIEYFVTTRLTELEYYRQLPEKD
                     LPPCSYKDKWQKPDSYDVCKNGKSIHSFLNEEYAYTFMQRNNLDSSYEIVKTVNKPKR
                     CERYCPVKDICSQYLKECQNG"
     gene            889..1221
                     /locus_tag="PLU55_04550"
     CDS             889..1221
                     /locus_tag="PLU55_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87360.1"
                     /translation="MVNQDIVKEVKVHNSQIYNGQWDFKIVFPNSSIAYYFITYKELT
                     KEEITNFLVKRMKTYIAYEIYFADDKREFIDLQELTTIKNNLDLRRAQAIVNKYLVYN
                     PQSSIIGV"
     gene            1224..1499
                     /locus_tag="PLU55_04555"
     CDS             1224..1499
                     /locus_tag="PLU55_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87361.1"
                     /translation="MKITKVQAVKSNITYEPKSKYGLGQMIKITAEVYESDGSIKDYN
                     AEFLGYITSIQTTRDNKHNISYVYTVQCPKLHNYLSFPEEQILPVEE"
     gene            1500..1970
                     /locus_tag="PLU55_04560"
     CDS             1500..1970
                     /locus_tag="PLU55_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87362.1"
                     /translation="MPNRKDYIKIGNYRLDIDYYYINKDNRLCAVGKDEEPKDITRNK
                     PDLDLPDDIPETIVDTVNKFITVRKQQYPNIPSVKNANFAASMKEVYKLIKQGYQEDL
                     ICSAIEVALYNDFWKKNLLVLTQLNSKCKSGLTKFEHLLILLKRPEKIDDYEEL"
     gene            1970..3280
                     /gene="gvpD"
                     /locus_tag="PLU55_04565"
     CDS             1970..3280
                     /gene="gvpD"
                     /locus_tag="PLU55_04565"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpD"
                     /protein_id="HPJ87363.1"
                     /translation="MANTTSNIGSEKKLLSILLNNPEKALASISLLNHKCFTNIDNRN
                     IYNAIWTVADAGESVDTVSIIHQLRSQGILDTTLKQYLNDIVEMQTNPEHFISLVKEV
                     IDLWKIRETNQMIEKMGQAIQEGTSYNNLKSIMNRYSHLDTSTNGDKPETMLSIINKA
                     IRETEDAIKTGTQRELGLRFGYPKLDKVMSLRPGNTYCIAARPAMGKTSFVLNIIDNL
                     LQEGKRIYLVSTEMQKEEIMEKFITMKQAISNEDFRSLPEEHKLARYKELYSYNAAKN
                     SELLIDSSSFQLYDTIARIRALHKEKPLDLVVIDYLQQLELDGNINRVQVVSEITRKY
                     KLLSSELKIPIIEVSQLSRKVEERAVKIPILSDLRESGSIEQDMSGVIFIYRPAYYGI
                     EEDDEGNDISNLAEIMIAKNKFGRRGKIKYIFDNETTLFREIKK"
     gene            3277..3546
                     /locus_tag="PLU55_04570"
     CDS             3277..3546
                     /locus_tag="PLU55_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87364.1"
                     /translation="MITTDNIYYRSYGDKKEKRLAKQELKAIAKKIIDSATSWTEALI
                     INDNRGDYKMLLLFIVKSSLERDELLRESKWHYSDIIYSLVKAIK"
     gene            3543..4049
                     /locus_tag="PLU55_04575"
     CDS             3543..4049
                     /locus_tag="PLU55_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87365.1"
                     /translation="MIKLIAAIDPGRNGGICVIDKKNTIRYLNVFSSKKEDIDKSLKE
                     VSYLTNVLFLESAISMPTNRTTCNNLFFLNGYITGYAEGLGVQVISCSPRHWKNCMRL
                     SRDKDESIIKAMELFNIEKELLSYPKYSRDGLAEALLLGYYAFHYFPFYPEKDQIDLW
                     KEIVSQYE"
     gene            4042..4257
                     /locus_tag="PLU55_04580"
     CDS             4042..4257
                     /locus_tag="PLU55_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87366.1"
                     /translation="MNNKLTAGETILEAMVYHNLCLGDLVKAIPEVTEDYLLGILQDR
                     YTITPEVAVKLARLLDLSSDYILRLQH"
BASE COUNT         1644 a          610 c          777 g         1227 t
ORIGIN      
        1 ataaaaagat gagtttaacc aataaagcag gtatgcctgc tatcttatat gaggcattat
       61 gttcattaga agccaataat gaacatcttc acaatcaagg ttggagtgtc accgaaatct
      121 tggaacctac cagaattgca gttctgaaaa agaaatatgc tcctaagtta gaaatagacg
      181 ctatagacca aatggcatct gcgttaggaa ctgcagtgca tatacttttg gaacaaggtc
      241 acaaaggcaa agaagatttg ggtatctttg tagaaaagaa agtcaattta gaaatcaatg
      301 gtgttatcct gtctggaaag atagactatt ataatcaaaa tgatgaattg atagttgatt
      361 acaaatatca gaatgtccaa agctgggtaa atattcaaga taagttagat tatccaacct
      421 ggcaacttaa cttatacaaa tacttactcc ataaaaataa ctatcctgtt aaacatttac
      481 aagtgttatt tatcttcaaa gattttagag agtatgaagc aggaaaattt aagagttatc
      541 ctgaaacact ctggaagcta atcaatattg aaagtaaacc agaagaagag atagaatatt
      601 ttgttactac cagattaaca gaattggaat attatagaca gctaccggaa aaagatttac
      661 caccttgttc ttataaggat aagtggcaga aaccagatag ttatgatgtc tgcaagaacg
      721 gcaaaagcat tcattctttt ctcaatgaag agtatgccta tacctttatg cagaggaaca
      781 atctagatag cagttatgag attgttaaga cagtaaataa acctaaaaga tgtgaaagat
      841 attgtcctgt taaagatatc tgcagtcaat atctgaagga atgtcagaat ggttaatcaa
      901 gatattgtta aagaagttaa agtccataat agccaaatat ataatggaca atgggatttt
      961 aagattgtgt ttcccaatag ttccattgct tattacttca ttacctataa agagttaacc
     1021 aaagaagaaa taaccaattt cttagttaaa agaatgaaaa cttacatagc ttatgaaatc
     1081 tattttgcag atgataaaag agagtttata gatctgcaag aattaacaac cattaaaaat
     1141 aacttagact tgagaagagc tcaggctatt gttaataagt atctggtcta taacccacaa
     1201 agttcaataa taggagtata aaaatgaaga taaccaaagt tcaagctgta aaaagcaata
     1261 ttacttatga acctaagtcc aaatatggtc tgggtcaaat gataaaaatt actgccgaag
     1321 tttacgaatc tgatggcagt attaaagact ataatgcaga gtttttgggg tatatcacca
     1381 gtattcaaac aacaagagac aataaacata atataagtta tgtctatact gtccaatgtc
     1441 ctaaactgca taattatctt tcttttccag aagaacaaat cttaccagta gaggaataga
     1501 tgccaaacag aaaagactat attaagatag gaaattacag actggatatt gattattact
     1561 acatcaataa agataacagg ctgtgtgcag taggcaaaga cgaggaacct aaagatataa
     1621 cccgaaacaa acctgatctt gatttaccag acgacattcc tgaaactatc gtagatactg
     1681 ttaataagtt tattacggta agaaaacaac aatatcccaa tattccctca gttaagaatg
     1741 ccaactttgc tgcctctatg aaagaggtat ataaactaat taaacaagga taccaagaag
     1801 atcttatttg tagtgcaata gaagtagcct tatacaatga tttctggaaa aagaatttat
     1861 tggttctgac ccaacttaat tccaaatgta aaagcggttt aaccaaattt gaacatcttt
     1921 taatactctt aaagagacca gaaaaaattg atgattatga ggagttataa tggcaaacac
     1981 aacctccaat ataggatctg agaaaaaact attatccata ttacttaata atccagagaa
     2041 agctttggct tctatttctc tgttaaatca taaatgtttt accaatatag ataaccgcaa
     2101 tatttacaat gctatctgga ctgtagcaga tgcaggtgaa tctgtggata ctgttagcat
     2161 tattcatcaa cttagaagtc aaggtatatt agatactact cttaagcaat atttgaatga
     2221 tatagtagaa atgcaaacta accctgaaca ctttatctcg ttagtaaaag aagtaataga
     2281 tctatggaaa atcagagaaa ccaatcagat gatagaaaaa atgggtcagg ctattcagga
     2341 aggaacaagt tacaataacc tgaaatccat aatgaacagg tattctcacc tagatacttc
     2401 tactaatgga gataagcctg agactatgtt atccattatc aataaagcca taagagaaac
     2461 cgaagatgcc attaaaacag gaacacaaag agaattggga ttaaggttcg gttatccaaa
     2521 actagataaa gtgatgtctt taagacctgg aaatacttat tgtattgcag caagacctgc
     2581 tatggggaag accagttttg tcttaaatat tattgacaac ctcttacaag aaggaaagag
     2641 aatttatttg gtctctaccg aaatgcagaa ggaagagatt atggaaaagt tcattacaat
     2701 gaaacaagca atatccaatg aggatttcag atctcttcct gaagagcata agctggcaag
     2761 atataaagaa ttatacagtt acaatgcagc taagaactca gagttattga tagatagcag
     2821 ttcttttcaa ttatatgata ctatagcccg aataagagcc ttgcataaag aaaaaccttt
     2881 agatctggta gtgatagatt atttacaaca gttagaacta gatggcaata tcaatagagt
     2941 tcaggttgtt tctgagataa ccagaaagta taagttatta tcatccgagt taaagatacc
     3001 gattatagaa gtatcccaat taagcaggaa agtagaagag agggctgtta aaattcctat
     3061 cctttccgat ttaagggaaa gtggttctat tgaacaagat atgagtggag taatctttat
     3121 ctacagacct gcttattatg ggatagaaga ggatgatgaa ggcaatgata taagcaatct
     3181 agcagagatt atgattgcta aaaacaaatt tggaagaaga ggtaaaatca agtatatctt
     3241 tgacaatgaa acaactttat tcagggagat aaaaaaatga taacaaccga taatatttat
     3301 tacagaagct atggcgataa aaaggaaaaa cgactcgcca aacaagagtt aaaagcaatt
     3361 gccaaaaaga ttattgatag tgctacaagt tggacagaag ccttaataat caatgataac
     3421 agaggagatt ataagatgct tctattgttc atagtaaaga gttctctgga aagggatgaa
     3481 ttattaagag aaagcaaatg gcattatagc gatattatat atagtttggt aaaggcaata
     3541 aaatgattaa actaatagca gctatagatc caggtagaaa tggtggtatc tgtgtaattg
     3601 ataagaaaaa taccattcgt tatcttaatg tgtttagttc caagaaagaa gatattgata
     3661 aaagcctgaa agaagtcagt tatctcacta atgtcctctt tttggagtct gccattagta
     3721 tgcctaccaa tagaactact tgtaacaatc tctttttctt aaatggctat attacaggtt
     3781 atgcagaagg attaggtgtg caagtaataa gttgttcacc caggcattgg aaaaattgta
     3841 tgaggttaag tagagataaa gatgaaagca ttatcaaggc tatggaactc tttaatatag
     3901 aaaaagagtt attgtcttat cccaaatata gtagagatgg attggcagaa gctttattat
     3961 taggatatta tgctttccat tatttccctt tttatccaga aaaagaccag atagatttat
     4021 ggaaggagat agttagccaa tatgaataac aagcttaccg caggtgaaac aatattggag
     4081 gctatggttt atcataacct ctgtttggga gatttggtaa aggcaattcc agaagtaaca
     4141 gaagattatt tattgggaat cttgcaagac agatatacta ttactccaga agtagcagtt
     4201 aagttagcaa gattattgga cttatcatca gattatattt taagattaca gcattaag
//
LOCUS       DAOPCH010000062         4252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_17076_length_4252_cov_5.763402, whole genome shotgun sequence.
ACCESSION   DAOPCH010000062 DAOPCH010000000
VERSION     DAOPCH010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17076_length_4252_cov_5.763402"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2020)
                     /locus_tag="PLU55_04585"
     CDS             complement(<1..2020)
                     /locus_tag="PLU55_04585"
                     /inference="COORDINATES: protein motif:HMM:NF017053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030908 - protein splicing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing homing endonuclease"
                     /protein_id="HPJ87367.1"
                     /translation="MSIKTEQDKAIIEKINEDYKSLSLEERQQLYTYFFDKNTRKEFK
                     EKLENLIFFKKPPTPEEFLDPANGWLPENIISSIYPYIKEQFIDVLSQEKVYNKVCQY
                     GATRLGKCEGKDTPILMYDLSVKKIQDIKVGDLLMGDDSTPRRVLHLHRGYGKLYKVK
                     QNKADDYIVNENHILTLQYTNKGLRKNRNNSPDKHNGKTIDIAIKDYLVLSKDHKHRL
                     KGIKAPLHFEENPVEIEPYWLGLYLGDGSENCTEITNVDKEIIDYIYDYAERLNLKVS
                     KHFQNNNAHGYRIIAKERSDSKNKEVNTLYFHLKDTYNILYNKHIPIEYLRNSEEVRL
                     QLLAGLLDSDGHLNKSGNVFEIAQKRKDLAEQICFLCRSLGFRATIKEKITNFNTLVY
                     RITIFGDLYKIPTRIPRKQSKQKARINPLRTGIEVVEHGEGEFFGFELDGNRRYLHSD
                     LTITHNTFCARLLILYTFVFIHHLREPALYYNLSPLTDLCIYIISFKLDKTRQLYLEP
                     IYKIMRKSKRFKQVKFVDKVKEEQEQVGIDTVVWSKASESGEITLASGLQLHMGNDDA
                     LGIIGGDILQCYLGEINWFIEYAGASEESIFRLYTDIGDRIEGTVGKSYLAFTYLDSS
                     ANDADSLIEKHIINELQYRDDVYFKWQSRWEARPYLFPKWQQTGETFKV"
     gene            complement(2080..2370)
                     /locus_tag="PLU55_04590"
     CDS             complement(2080..2370)
                     /locus_tag="PLU55_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87368.1"
                     /translation="MTIKAQKCIFEQKKSLKESNAKSKILSDLDYALKELKTEVLNKI
                     KRLKENEFEFIVQEYGDDFPMILISRYMENPSPNIKKKLSKFRRSSLWQKIY"
     gene            complement(2406..2579)
                     /locus_tag="PLU55_04595"
     CDS             complement(2406..2579)
                     /locus_tag="PLU55_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87369.1"
                     /translation="MKKLREFELSRNDLLYDKVEASSYKEAIEYFKKAHKGKGNFEIT
                     VIGDSEKIKRIRL"
     gene            complement(3064..3201)
                     /locus_tag="PLU55_04600"
     CDS             complement(3064..3201)
                     /locus_tag="PLU55_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87370.1"
                     /translation="MKKLKRYIRMDEHLYEMANLTKEDTGLKNCLDFSQNRKRKTLGK
                     N"
     gene            3415..3582
                     /locus_tag="PLU55_04605"
     CDS             3415..3582
                     /locus_tag="PLU55_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87371.1"
                     /translation="MKCSFCSNDSIKVHAIENIISSEELKRFEKVKKNSKVCVECRND
                     LLFSVVAMSEY"
     gene            complement(3591..>4252)
                     /locus_tag="PLU55_04610"
     CDS             complement(3591..>4252)
                     /locus_tag="PLU55_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87372.1"
                     /translation="SNSFSDNLENKQKNSYNKKDIVKSILLNKIGKTPYKIFARKCEI
                     RKVNHSEATQFLDNNHLQGKCISSQHYGLYFNNELVCLLSISKSRFSKKYEWEITRFC
                     NKNYYSIVGGFSKLLKHFIKNHNPNNIVTYADKRLSLGGLYLKNDFKYLQDTPPNYYY
                     FYNTKDYVLFNRIAFQKHKLEKKLKTFSPERTEWENMMDNGYDRIWDCGNMVFEWKKE
                     G"
BASE COUNT         1351 a          672 c          490 g         1739 t
ORIGIN      
        1 caactttaaa agtttctcct gtttgttgcc atttaggaaa aagataaggt cttgcttccc
       61 atcttgactg ccatttaaaa tatacatcat ctctgtattg aagttcattt ataatatgtt
      121 tttcaataag tgaatcagca tcgttagcgg aagagtccaa ataggtgaaa gctaaatagg
      181 atttaccaac tgttccttct attctatccc ctatgtccgt ataaagtcta aatatgcttt
      241 cttccgaagc ccctgcatat tcaatgaacc agtttatttc ccctaaataa cattgaagaa
      301 tatccccacc tatgatacct aaagcatcat cattacccat gtgtaactgt aaaccacttg
      361 caagtgtaat ttctcctgat tcacttgctt tactccaaac aactgtgtct atacctactt
      421 gctcttgttc ttctttaact ttatctacaa atttgacctg cttaaatctt ttagattttc
      481 tcattatttt gtaaataggt tcaaggtata actgccttgt tttatcaagt ttaaaagata
      541 ttatataaat acataaatct gtcaaaggtg aaagattgta gtataatgcc ggttctctta
      601 aatgatgaat aaaaacaaaa gtataaagta taagtaatcg ggcacaaaaa gtattgtggg
      661 taattgttaa atctgaatga agatatcttc tattcccatc aagttcaaat ccaaaaaatt
      721 caccttcacc atgttcaaca acttcaatac ctgttcttaa aggatttatt cttgctttct
      781 gtttactttg ttttcttggt attcttgtag gtattttata taagtcacca aatatagtta
      841 tcctgtaaac aagagtatta aagtttgtaa ttttttcttt aatggttgct ctaaaaccta
      901 aacttctaca caagaaacaa atttgttctg ctaaatcctt cctcttctgt gctatttcaa
      961 aaacattgcc tgacttgttt aaatgaccat ctgaatctaa taatcctgca agtaattgta
     1021 atctcacttc ttctgaattt cttaaatatt ctattggtat atgtttatta tataaaatat
     1081 tatatgtatc ttttaaatga aagtaaagtg tattgacttc tttattttta ctatcacttc
     1141 tttcttttgc tataatccta taaccatgtg cattattgtt ttgaaaatgc ttgcttactt
     1201 ttaaatttaa tctttcagca taatcataaa tataatcaat aatttcttta tctacatttg
     1261 ttatttcagt acaattttct gaaccatctc cgagataaag accaagccaa taaggttcta
     1321 tttctacagg attttcttca aaatgtaatg gtgcttttat tcctttaagt ctatgtttat
     1381 gatctttact taaaactaaa taatctttaa ttgctatatc aatagttttc ccattatgtt
     1441 tatccggtga attattccta ttttttctta aacctttatt tgtgtattgt aatgtcaata
     1501 tatgattttc atttacaata taatcatcag ctttattttg tttaacttta tataattttc
     1561 cataccctct atgaaggtgt aatactcttc ttggtgtgct gtcatccccc ataagtaaat
     1621 caccaacttt aatgtcttgt atctttttaa cagataagtc atacattaaa ataggggtat
     1681 ccttaccttc acattttcct aatcttgtcg ctccgtattg acaaacttta ttataaactt
     1741 tttcttgtga aagaacatct ataaactgtt ccttaatata aggatatatt gaagaaataa
     1801 tattttcggg caaccaacca ttagcagggt ctaaaaattc ttcaggtgtt ggtggttttt
     1861 taaaaaaaat taaattttca agtttctctt taaattcttt tcgggtgttc ttatcaaaga
     1921 agtatgtgta taactgttgt ctttcttcta gggaaagact tttataatct tcatttattt
     1981 tttctataat tgctttatct tgttctgttt taatagacac aaaaaaacct ctacaaatat
     2041 tagtagaggt tttagtgttt gtttattttg aaaaataaat tagtatattt tttgccaaag
     2101 actacttctt ctaaatttag acaatttctt ttttatattt ggacttggat tttccatata
     2161 ccttgatata agtatcattg gaaaatcatc cccatattct tgtacaataa attcaaattc
     2221 attttctttc aaccttttaa ttttatttaa aacttcagtt tttaattctt ttaaagcata
     2281 atctaaatct gaaagaattt tagactttgc attggattct tttaatgatt ttttttgttc
     2341 aaaaatacat ttttgtgctt taattgtcat tttattttaa acctctcttc ttttattagt
     2401 ataactcaaa gtctaattct ttttatcttt tcggaatccc ctattacagt tatttcaaaa
     2461 ttaccctttc ctttatgagc ttttttgaaa tactcaatag cttccttgta tgaagaagct
     2521 tctactttat catacaacaa atcatttctt gacaattcaa attctctaag ctttttcatt
     2581 ggttgttctc ctttatagta taagatttca tagtaaacta ttactttctt tcaacgcttc
     2641 aacgaaaacg ccttttgaaa tgtattcaat tggtcttatt ttttctccac gtttgtactc
     2701 gccgtactcc ggcgagaaat acttcaaaat atttaatatt ttattttaca ttatctttaa
     2761 tgtgagcccc cattttatat aaaaaaatat ttatcgtatt agtttattta attagtaaat
     2821 taccatatta tttaatattt tactgtattt tcattaaatt aactaaatac ttttttaaga
     2881 tttttaaaaa ctaatttaag gctcatttcc ccgttactat tccaatattg caaaagcaaa
     2941 tctttattca aaacaatcca cttctgcaat tttttaaagt ttggaatttc aacatttttc
     3001 ttaaccacta ttcttggaac gtcctcaata gtaacaggaa taaattccat attgacttga
     3061 actttaattc ttgcccaatg tttttctttt cctgttctgg gagaaatcca aacaattttt
     3121 tagtcctgta tcttctttag ttaggtttgc catttcatat aaatgttcat ccattcttat
     3181 atatctttta agttttttca taatatcttc ctcatttata atattagttt ataatatgcg
     3241 attattttta tcaacaacaa aatttaaaaa aaaatagcaa atgtttgttt taactaactt
     3301 tactgttttt tctaaaaaaa atgtttaagt gttttcacat ctttccgata ataataatgc
     3361 aaggaagcaa aaaaaataaa agtcaaggaa gacacaacaa caaagaggtt gtgtatgaaa
     3421 tgttcattct gttctaatga ttctatcaaa gtccatgcta ttgaaaacat tatttcaagt
     3481 gaggaattaa aaagatttga aaaggtaaag aaaaacagta aagtttgtgt agaatgtaga
     3541 aatgatttac tattttctgt tgttgctatg agtgagtatt gaaagagggg ttaaccctct
     3601 tttttccatt caaaaaccat attaccacaa tcccaaattc tatcataacc attatccatc
     3661 atgttctccc attctgttct ttcaggagaa aatgttttta atttcttttc gagtttatgt
     3721 ttctgaaaag ctattcgatt gaaaagaaca taatcttttg tattgtaaaa ataataataa
     3781 tttggtgggg tatcctgtag atatttaaaa tcatttttta aatatagtcc ccccaaacta
     3841 agtcttttat cggcataagt gacaatgtta ttaggattat gattttttat aaagtgtttt
     3901 aataatttag aaaatccccc aacaatagaa taatagtttt tattacaaaa tcgagtaatt
     3961 tcccattcat attttttact gaatcttgat ttacttatag acaataaaca aaccaactca
     4021 ttattaaaat ataatccata gtgctgtgaa cttatacatt tcccttgtag atgattattg
     4081 tctaaaaatt gtgtagcttc agagtgattc acttttctaa tttcacattt acgtgcaaaa
     4141 atcttataag gagttttacc tattttgttt aaaagtattg attttactat atctttttta
     4201 ttatagctgt ttttctgttt gttttcaaga ttgtccgaaa acgaatttga tt
//
LOCUS       DAOPCH010000063         4155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_17577_length_4155_cov_4.177317, whole genome shotgun sequence.
ACCESSION   DAOPCH010000063 DAOPCH010000000
VERSION     DAOPCH010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17577_length_4155_cov_4.177317"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PLU55_04615"
     CDS             complement(<1..592)
                     /locus_tag="PLU55_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87373.1"
                     /translation="MRLIKLNNAVSMYPEPVFGTVKAEQRVMAISIDKETKNPYLGLV
                     REITKSQGTPDKPGFIDESKLIIVESQDALKWKKKADLKIKGINKIIKEISGKDKYFI
                     GLEDPDIINEGKTKHVYFTIAFKYKKKIGYSVYLGHAQGNSIENLTATKPVLSPIQKF
                     RGFKEVTISPVNKKGYRINLSEAQLVINNDELSVTIS"
     gene            complement(661..1848)
                     /locus_tag="PLU55_04620"
     CDS             complement(661..1848)
                     /locus_tag="PLU55_04620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPJ87374.1"
                     /translation="MRCLLLYPYPVESDGVSLQGHYLARGLRNLGVEVIECHYEESIQ
                     KEFYMKYSKPDFVIGIGFWGNVPEIVVEPQKHGLTVIPWFNADGWVANYREEFERLRL
                     MFTTSAWVRSIYERDGVDVSKIIPMHIGIDTNLFKPNNNPEANLRLRRLLGVKDHEIM
                     IFTMGGDVTSKGAQEMMKALAEINKEFKDWVYVCKSWPSDCAEKWHEFEETLAEELDI
                     RDKIIFLDDIFSPEFCADILNACDIYAAPSRIEGFGMIQVEAMACGKPVISINVGGPA
                     ETIIHGKTGLLARVAEEIKLTEEWVYPHMGFNEQSKMKFTTPKTFGYRANVEDLTRYT
                     LMLLKDKNLREEMGKAGRAHAVANFDYNVIAKRMLDVIKEEFDMNNEEQDEEEIPKLK
                     EYF"
     gene            complement(1857..2537)
                     /locus_tag="PLU55_04625"
     CDS             complement(1857..2537)
                     /locus_tag="PLU55_04625"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPJ87375.1"
                     /translation="MASKKKKVVKKRKKTKEKRIVKIPGEKKEVLVIIAHPDDETIWM
                     GGTLLKIKEKQDSNVTLICLCRKNDKDRYPKFQKACKIFGVKGYISDLDDSEDGGQYK
                     EILPEDIIKRISPITNNKLYTTLYTHNKNGEYGHIRHIQVNQAIEEMLSKGLILAEEV
                     YFFSYKKVENKYQGYAIYNSNADKLIKLKKPYLKMKKRIIEEVYGYENGGFEEKSCRE
                     IEAFDLRQ"
     gene            2604..3533
                     /locus_tag="PLU55_04630"
     CDS             2604..3533
                     /locus_tag="PLU55_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPJ87376.1"
                     /translation="MKFDVVSFGSAVVDVFIQTDVSEKKGFMYYPIGEKILIKELRFD
                     VGGAGTNTSVAFSRLGLKAGCICEFGDDENGRRIKSLLKKEKVKFLGKTVRGELTGYS
                     VILDSKGGDRTILTYKGANDEVSLRDISKFNTKWLYFGSLLGKSFETQKQLAVKLKKK
                     KGIKLAYNPSSYQIKAMNITSLLKITDILVVNKEEAEMLLKRYKPKEKDLLLGLHKIT
                     KGIIVITDRDRPTLCYDGNRKYSIKPHKEIKVVERTGAGDAFASGFVAGQIAGKSITR
                     SLELGIKNSEAVIQNFGAKTNLIRMKLKHREQL"
     gene            3550..>4155
                     /locus_tag="PLU55_04635"
     CDS             3550..>4155
                     /locus_tag="PLU55_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HPJ87377.1"
                     /translation="MKPISLSKITTRGKALFLAYDQGLEHGPSDFDDKNVDPKYIIEI
                     AKKGKYNGIVFQKGIAEKYNQEIKASKVPLIVKLNGKTNLFKGEPIARQLCTVDEAIK
                     LGAKAVGYTIYIGSDSEDESFQEFQNIQREAHEKGLPVIAWIYPRGKSVEKKKPEELM
                     AYAARTALEIGADIVKIQYTGKFKDLKWAVKSAGKTKVVIAG"
BASE COUNT         1336 a          610 c          647 g         1562 t
ORIGIN      
        1 ctgagattgt aactgataat tcatcattgt ttattactaa ctgagcttca cttaagttta
       61 ttctgtatcc ttttttattt actggagaga ttgttacttc tttaaaaccc ctaaattttt
      121 gaataggaga gagtacaggt tttgttgcag ttaaattctc aattgaattt ccttgagcat
      181 gacctaagta aacagaatat cctatctttt ttttgtattt gaatgcaatt gtgaaataaa
      241 catgttttgt ttttccttcg tttattatgt cggggtcttc tagtcctata aagtatttgt
      301 cttttccaga aatttctttt attattttat taattccttt tatttttaaa tctgctttct
      361 ttttccattt aagtgcatct tggctttcaa caattattaa tttactttca tcaataaaac
      421 ctggtttgtc aggtgttcct tgacttttgg ttatttctct tactaaacct aaatatggat
      481 ttttggtttc tttgtctata gaaatagcca tgactctttg ttctgctttg actgttccaa
      541 aaacaggttc aggatacata gaaacagcat tatttaattt gattaacctc atataagaac
      601 atagaataga ttattattaa actttattta tattttcctg tgtataatag agtttctttt
      661 ttagaaatat tcttttaatt taggtatttc ttcttcatct tgctcttcgt tattcatatc
      721 aaattcttct tttattacat ctaacattct ctttgctata acattgtaat caaaattagc
      781 aacagcatgc gctctacctg cctttcccat ttcttctctt aggtttttgt cctttaggag
      841 catgagagta tatcttgtta aatcttcaac attagctcta tatccgaaag tttttggtgt
      901 tgtgaatttc attttactct gttcattaaa acccatgtga ggatataccc attcttctgt
      961 taattttatt tcttctgcaa ctcttgctaa taatcctgtt tttccatgga ttattgtttc
     1021 tgcaggtcct ccaacattta ttgaaataac tggttttcca caagccattg cttcaacttg
     1081 tatcattcca aatccttcaa ttcttgaagg tgcagcgtaa atatcgcagg cattaagtat
     1141 atctgcacaa aattcaggtg agaaaatatc atctaaaaaa attattttat ctcgtatatc
     1201 taattcttct gcaagtgttt cttcaaattc atgccatttt tctgcacaat cacttggcca
     1261 agatttacaa acataaaccc aatctttgaa ttctttatta atttcagcta atgctttcat
     1321 catttcttga gctcctttag atgtaacatc tcctcccata gtgaatatca ttatttcgtg
     1381 gtcttttact cctaaaagtc ttctgagtct aagattagct tctggattat tattaggttt
     1441 gaataggttt gtatcaattc ctatatgcat aggaataatt tttgatacat caactccatc
     1501 tctttcatag atgcttctta cccaggcact tgttgtgaac attaatctta atctttcaaa
     1561 ttcttctctg taatttgcaa cccaaccatc tgcattaaac caagggataa cagttaaacc
     1621 gtgtttttga ggttcaacta ctatttcagg aacatttccc cagaatccta ttccaataac
     1681 aaagtccggt tttgaatatt tcatataaaa ttctttttga atactttctt cataatgaca
     1741 ttctataact tctactccaa gatttcttaa tccacgagca agataatgtc cttgaagact
     1801 tacaccatca gattcaacag gataaggata taatagtaaa catctcatct tagtttttat
     1861 tgtcttaaat caaatgcttc aatttctctg caagattttt cttcaaaccc tccattctca
     1921 tatccataaa cctcttctat tatccttttc ttcattttaa gatagggttt ttttaatttt
     1981 ataagtttat ctgcatttga gttatatatt gcatatccct gatatttgtt ttccactttc
     2041 ttgtaagaga aaaaataaac ttcttctgct agaattaaac ctttacttag catctcttca
     2101 attgcttgat ttacttgaat atgccttata tgtccatatt ctccattttt attgtgagtg
     2161 taaagggttg tgtagagttt gttattggtt attggagaga ttctcttgat tatatcttca
     2221 ggtaagattt ctttgtattg tcctccatct tcactatcat ctaaatcaga gatatatcct
     2281 ttaactccaa agattttaca agctttttga aatttaggat atctgtcttt atcgtttttt
     2341 ctacataaac atattaaagt tacatttgaa tcttgttttt cttttatttt aagaagagtt
     2401 cctcccatcc agattgtttc atcatcagga tgagctataa taaccaaaac ttcttttttc
     2461 tctccaggta tctttactat tcttttttct ttggtttttt ttcttttttt gaccaccttc
     2521 ttttttttac ttgccatact catctaacag gaaagttttt aattaaataa atctttctaa
     2581 agttgtaaag aggatgaata aagatgaaat ttgatgttgt gagttttggt tctgctgttg
     2641 tagatgtatt tatccagaca gacgtttctg agaaaaaagg atttatgtat tatccaatag
     2701 gtgaaaaaat tcttattaaa gaattaaggt ttgatgttgg aggagcaggt actaatactt
     2761 ctgttgcttt ttctagatta ggattaaagg caggttgtat atgcgaattt ggagatgatg
     2821 aaaatgggag aagaataaaa agtttgctta aaaaagagaa ggttaagttt ttaggtaaga
     2881 cagtgagagg agaattaaca ggatattcag ttatattaga tagtaaagga ggagatagaa
     2941 ctattcttac atataaagga gctaatgatg aggtgagttt aagagacata agtaagttta
     3001 atacaaaatg gttgtatttt ggttctcttt taggaaagag ttttgagacc caaaaacaac
     3061 ttgcagttaa attaaaaaag aaaaaaggaa ttaaacttgc atataatcca agtagttatc
     3121 aaataaaggc tatgaatatt acttctttgt taaagataac cgatatttta gttgtgaata
     3181 aagaagaagc tgaaatgttg cttaagagat ataaacctaa ggaaaaagat ttgcttttag
     3241 gtttacataa aattacaaaa ggaataattg ttataactga tagagatagg cctactcttt
     3301 gttatgatgg aaatagaaaa tattctatta aacctcataa agagattaaa gtcgttgaga
     3361 gaacaggtgc tggagatgct tttgcttctg gatttgttgc aggacagata gcaggaaaat
     3421 caattacaag gtctttggaa ttgggaataa aaaattctga agctgttata caaaactttg
     3481 gagctaaaac taatttgata agaatgaaat taaaacatag agagcaatta taataaaaga
     3541 agtattaaaa tgaaaccaat aagcttaagt aaaataacga caagaggaaa agcacttttt
     3601 cttgcatatg atcagggatt agaacatggt ccaagtgatt ttgatgataa gaatgttgac
     3661 cctaaatata taattgaaat tgctaaaaaa ggaaaataca atggaattgt atttcagaaa
     3721 ggaattgcag agaaatacaa tcaagaaatt aaagcaagca aagttccttt aattgtgaaa
     3781 ctgaatggaa aaacaaattt gtttaaagga gaaccaatag caagacaatt atgtactgtt
     3841 gatgaagcta ttaagcttgg agctaaagca gtgggttata ctatttatat tggaagtgat
     3901 tctgaagatg aatcttttca agaatttcaa aatattcaaa gagaagcaca tgagaaagga
     3961 cttccagtta ttgcttggat ttatccgaga ggaaaatcag ttgaaaagaa gaaacctgaa
     4021 gagttaatgg catatgctgc aagaacagca ttggagattg gggcagatat tgttaagatt
     4081 caatatactg gaaaatttaa agatttaaaa tgggctgtta agtctgcagg taaaactaaa
     4141 gttgttattg ctgga
//
LOCUS       DAOPCH010000064         3505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_21555_length_3505_cov_4.136812, whole genome shotgun sequence.
ACCESSION   DAOPCH010000064 DAOPCH010000000
VERSION     DAOPCH010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21555_length_3505_cov_4.136812"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..697
                     /locus_tag="PLU55_04640"
     CDS             89..697
                     /locus_tag="PLU55_04640"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPJ87378.1"
                     /translation="MVNKVITFKPRERLLKALIEDKIPKSILSLSGSVGLDYKNTYNY
                     IKEFATSGIINQNSVGNTTLVEINLSLNSEIYSVEKKRTDEFLSKNQRLKVIKKYTEE
                     INYPFLIVLIFGSYAKNEKTENSDVDICIISDNKDKSKELQEKLHLLSLRLEIHEFTI
                     KEFISMIEKTQNNLGHEIIKSNIILYGIENYYNLISKWMKKE"
     gene            679..1200
                     /locus_tag="PLU55_04645"
     CDS             679..1200
                     /locus_tag="PLU55_04645"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HPJ87379.1"
                     /translation="MDEKRIKQAENNFKAYLTEGKIKKVSSQIPLIYSTYLRNARESL
                     KVANQLFENKTSSLWVVVTSYYSMFYMACAYLYKIGYKPGDEIVHQVVNESLISLGRN
                     KIKNYLLENYSEEKEKALIIVDSYLDNYEREKAKRASFQYQTTESIKESKAQTSLNRA
                     KEFLTLMEGILDK"
     gene            1266..1553
                     /locus_tag="PLU55_04650"
     CDS             1266..1553
                     /locus_tag="PLU55_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87380.1"
                     /translation="MEGKNSWIAVTSFVLVICGFVWLIFSLIIFGGLFYRIFSVVQLV
                     AYILFISGILFGIVGLIHAIGTGLKGRGLAWVAFFLGILGFFVTILNTPLY"
     gene            1637..2089
                     /locus_tag="PLU55_04655"
     CDS             1637..2089
                     /locus_tag="PLU55_04655"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HPJ87381.1"
                     /translation="MKRVYLIGHGNLEIKEFVDLLIKNNIKTLVDVRSIPYSKYNSQF
                     NRENLEYELSEVGIEYKHLGNKLGGKIPEDFEKYIKSEEFNEGLNVLKEIIISSNSAI
                     MCAEIDYNKCHRKFIALRLNEAGFEVLAIGKKGIVQKVTQKTLTDIFQ"
     gene            2183..2851
                     /locus_tag="PLU55_04660"
     CDS             2183..2851
                     /locus_tag="PLU55_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87382.1"
                     /translation="MAISNKTYDALIIPTGETTFGDYSFPVTNKAIELFNSGKYGCLF
                     ITGGYSGFATAERKGKDSEGKETYDYILNKGIKGLDEKKVFCDDLSLESVGNFTFPIV
                     EQAPGNPNLRDFDNMKIVAKAGHIWRLIDYTKLVMPDKFKNGKIDFSAVPGEHNNGLT
                     ARVYHKGVLNAIGERRGAEEIHDFLINKHPFYSDGWYKKSVNRRKLEMSAIGGLWNLG
                     LKKE"
     gene            2959..3183
                     /locus_tag="PLU55_04665"
     CDS             2959..3183
                     /locus_tag="PLU55_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87383.1"
                     /translation="MKSRWLLLTWGFICSVIAFIPLYFFVYLKFKSAESTSSILSFSV
                     GIDMIILISLVLGFIVSGIIVILSRKKEIK"
     gene            3346..>3505
                     /locus_tag="PLU55_04670"
     CDS             3346..>3505
                     /locus_tag="PLU55_04670"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HPJ87384.1"
                     /translation="MVSEDKIDKFKEAARIIKSAKYLVVFTGAGISAESGVPTFRGDK
                     GIWKKYDSN"
BASE COUNT         1312 a          350 c          602 g         1241 t
ORIGIN      
        1 gtaatctcct agggaaaaaa gaaagataaa gaatctaata aaccctaaaa atcaaaacat
       61 ttaaatatgg taaatatacc atattattat ggtaaataaa gtaattactt ttaaacctag
      121 ggaaagattg ttaaaagcct taattgaaga taagattccc aagtcaatct taagtttatc
      181 tggttcagtt ggtcttgatt ataaaaatac atataattac attaaagaat ttgcaaccag
      241 cgggataata aatcaaaatt ctgttggaaa tactaccctt gttgagataa atctttctct
      301 gaactcggaa atctattctg ttgaaaaaaa gagaacagat gaatttttgt ccaaaaatca
      361 aagattaaaa gttataaaaa agtatacaga agagataaat tacccttttt taattgtctt
      421 gatatttggg tcttatgcaa aaaatgaaaa aacagaaaat tcagatgttg atatttgcat
      481 aatttcagat aataaagata aatcaaaaga attacaggag aaactccacc tattgtcttt
      541 gagactggaa attcacgaat ttacaataaa agagtttata tcaatgatag agaaaacaca
      601 aaataacttg ggtcatgaaa taattaaaag taatattatt ttgtatggga tagaaaatta
      661 ttataatctt atatcaaaat ggatgaaaaa agaataaaac aagctgagaa taattttaaa
      721 gcatatttga cagaaggaaa aataaagaaa gtatcatctc aaattccttt aatttattct
      781 acttatctaa gaaatgctag agaaagtcta aaagtagcta atcaactctt tgagaataaa
      841 acttcatctt tatgggttgt tgtaactagt tattattcta tgttctacat ggcttgtgca
      901 tatctttaca aaataggata taaacctgga gatgagatag ttcatcaagt agttaatgaa
      961 tctttgattt ccttggggag aaataagata aaaaactatc ttcttgagaa ttattctgaa
     1021 gagaaagaaa aagcattaat aattgtagat agttatctgg ataattatga aagagagaag
     1081 gctaaacgag caagttttca atatcagaca actgaaagta taaaagaatc aaaagcccag
     1141 acttctttaa acagagcaaa agaatttttg acattaatgg aaggaatatt agataaataa
     1201 agtagggtct ataaattata tcttgttttg ataaattttt taaatagtat tttactattt
     1261 aatttatgga aggaaagaat tcttggatag ctgttacatc ttttgtattg gtaatttgtg
     1321 gttttgtttg gctgattttt tcactaatta tctttggggg tttattttat aggatttttt
     1381 ctgtcgttca attggttgca tatattttgt ttatatctgg aattttattt ggtattgttg
     1441 gattaattca tgctatagga actggattaa aaggaagggg attagcttgg gttgcatttt
     1501 ttcttgggat tttaggattc tttgtaacta ttctaaatac tccactctat taataagata
     1561 aatcaatctt agttattttt agggattatc taattttagt aaattatata taataaaata
     1621 acaagttttt tacgacatga aaagagtata cttaattgga catggaaatc tggaaattaa
     1681 agagtttgtt gatttactaa ttaaaaataa tattaaaaca ttagttgatg taagaagtat
     1741 accttacagt aagtataatt ctcaatttaa tcgtgaaaac ttagagtatg agttatcgga
     1801 agtgggtata gaatataagc atttggggaa caaattgggt ggaaaaattc ctgaagattt
     1861 tgaaaaatat attaaatctg aagaatttaa tgagggatta aatgttctaa aagagattat
     1921 aatttcatca aattctgcta ttatgtgtgc tgaaatagat tataataaat gtcatagaaa
     1981 atttatagca ctaagactta atgaagctgg ttttgaagtt cttgcaatag gtaagaaggg
     2041 tattgttcaa aaagtaactc aaaaaacttt gactgatatt tttcaataat ttttgaactg
     2101 cttctaaatt ttttagtcac tataaaatta taacaaaata gtaaggttta aatatgctat
     2161 atttcttaga ttcttaggta aaatggcaat ttcaaataaa acttacgatg cacttattat
     2221 tccaacagga gaaacaactt ttggagatta cagttttcct gtgacaaata aggcaattga
     2281 attatttaat agtggcaaat atggatgctt atttattact ggaggttatt caggttttgc
     2341 aactgctgaa agaaagggga aagatagtga aggaaaagag acttatgatt atattctaaa
     2401 taaaggaatt aaaggactag atgagaagaa ggttttttgt gatgacttgt ctctggaaag
     2461 tgtagggaat ttcacatttc ctattgttga acaagcacct ggtaatccta atctcagaga
     2521 ttttgataat atgaagatag ttgcaaaagc agggcacata tggagattga ttgattatac
     2581 taaacttgtt atgcctgata aattcaagaa tggaaagatt gatttttctg ctgttccagg
     2641 agagcataac aacggattga cagcacgagt ttatcacaag ggagtactta atgctattgg
     2701 agagagaaga ggtgcagagg aaattcatga ttttttaatt aacaagcacc cattttattc
     2761 tgatggttgg tataagaaat cagttaatag aaggaaatta gaaatgtcag ctataggtgg
     2821 gctgtggaat ttgggattaa agaaggaata attagagttt tcaataaaat ttggttgtcg
     2881 attcttctga attttgaata aatattgtat actccttttt taataagatt tataaaattt
     2941 attttttgta tatatcatat gaaatctagg tggttattgt taacttgggg atttatttgt
     3001 tctgttattg catttatacc attgtatttt tttgtatatc ttaaattcaa atctgctgaa
     3061 tcaacatcaa gcattcttag ctttagtgtt ggaattgata tgataatttt aatttcattg
     3121 gttttaggat ttattgttag tggaataatt gttattttat ctaggaaaaa agaaataaag
     3181 tagagattat aacatctata tttaatttct aaatgttttg aataatttat ttttcattta
     3241 tttcttttta aaaaccttct ttttataaat aattataaat attatttatt catattattt
     3301 atcgacgata ataatcttta aataaacaat taactattta ggtatatggt aagtgaggac
     3361 aaaatagaca aatttaagga agcagcacga attattaaat ctgcaaagta tttggtagta
     3421 tttaccggtg caggaatatc tgctgaaagc ggtgtgccta catttagggg agataagggt
     3481 atatggaaaa aatatgattc taatg
//
LOCUS       DAOPCH010000065         3456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_21919_length_3456_cov_3.023817, whole genome shotgun sequence.
ACCESSION   DAOPCH010000065 DAOPCH010000000
VERSION     DAOPCH010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21919_length_3456_cov_3.023817"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PLU55_04675"
     CDS             <1..655
                     /locus_tag="PLU55_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPJ87385.1"
                     /translation="ICYNITKRKEDMWIAIAGCISDNLVPDFYSEFMKDFPELAKEDD
                     DAFNIFYGSEIGKIARIMGFGLKDKISNVILMIKFIISCKGPYDFLEENTKNKFIFKR
                     FYEIDKKYNKLLDRAKKVSNSSKLLFFKYAGDTSISSDIANALKYAYPDKFIVVVYNK
                     GAKANVSARGKDVKRVILKAIEGLESATGGGHDNAVGAQIRKEDVLEFEKRIKKLVN"
     gene            822..965
                     /locus_tag="PLU55_04680"
     CDS             822..965
                     /locus_tag="PLU55_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87386.1"
                     /translation="MVKKTLSVTVDEKVLEKWKKYTDEECINSSQLIEKLLKEHLKKR
                     GEK"
     gene            962..1513
                     /locus_tag="PLU55_04685"
     CDS             962..1513
                     /locus_tag="PLU55_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87387.1"
                     /translation="MKRRFLIGLISLVFILFLIGFVSTWVDNGNSSQSVDGCNQLNTT
                     GATYILTQNVSSNGTCFTITRPNITLDCNGYWINYSINGLENTYGVYTNQLNTTIKNC
                     NIVDGNWTGEAAGDRRGIYLKNVNGSTIFNNYIRVNNSAAIMLEGESSFNNVTNNIAY
                     GGLYGIDIQGHNNIISNNFANSS"
     gene            1538..>3456
                     /locus_tag="PLU55_04690"
     CDS             1538..>3456
                     /locus_tag="PLU55_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPJ87388.1"
                     /translation="MIIYGGQNNTLLDNALYGRYYGLSISNFANNTFGKNLSIIAEEH
                     SGITVEFGDNNTLTDFSVISRGNYGINLALSANNTFKDCTITNTGWYDVNVGWDPGCI
                     NNTLVNCSYSTERVPTSDGELIRKWYYQAYVNHTNGNPLSSVNISAYNTTGQLQFTTL
                     TNSSGWIPRQEVVEYIGSNQTRRYYSNYTINATSLGYEADSNTIDFTTTQNKLDDVFT
                     LQGQGLQCGELNSANTVYTLAQNVSSQGTCFTITQPNIILDCNGYWINYSINGSNNTY
                     GIYSNQFNTTIKNCNIVEGNFSNPVNNKHGIYLYGADNSTIFNNYINIQSSHPIDVRG
                     YSKYTNITKNTVISNSGPVVYLLDGAVNSVVENNTVISNGSAGIFLYGLCNNCAVINN
                     NVSNIGEGSEGIYVVGSNDVFLVNNTVTGRYTYSVNLYHSLNTLVLNQVAYGGKSGLN
                     MWDSNNSIIKDCINLSGYEYDVHIPSTYLGSSINNTFINCSYDSNKEYVDGEENELIR
                     KWYYQAHVNNTEGSPLSNANIAAYNSSGQLQFTTQTNSSGLIDRQEVTEYVNRGGTRS
                     YYNNYIINATSLGYETDSNIFNFTTTQNKLNDVFTLQGEGLHCGELNSANTVYTLAQN
                     VSSQGTCFTITQPNIT"
BASE COUNT         1313 a          387 c          590 g         1166 t
ORIGIN      
        1 tatttgttat aatattacta agagaaaaga agatatgtgg attgctattg ctgggtgtat
       61 ttcagataat ttagttcctg atttctattc agaatttatg aaagattttc ctgaattggc
      121 taaagaagat gatgatgcct tcaatatatt ttatggttct gagataggca aaattgcaag
      181 aattatgggg tttggattaa aagataaaat aagcaatgtt attttaatga ttaaatttat
      241 tatttcgtgt aaaggtccat atgatttctt agaagaaaac actaaaaata aatttatttt
      301 taaaagattt tatgagattg ataaaaaata caataagcta ttggataggg ctaaaaaagt
      361 ttctaactca agtaaacttt tatttttcaa gtatgctggt gatacaagta ttagttcaga
      421 tattgctaat gctttgaaat atgcttatcc agataaattt attgttgttg tatataataa
      481 aggggctaaa gcaaatgttt ctgcaagagg taaggatgtt aagagagtta ttctgaaagc
      541 aatagaaggt ctagagagtg caacaggtgg gggacacgat aatgctgttg gagctcagat
      601 tagaaaagaa gatgttctgg aatttgaaaa gagaattaaa aaattggtta attaatttat
      661 cttttatgtg tttttcttgt tttttaatta atttaaaatc cgatagattt aaataatata
      721 aatgttataa atactataaa taataaaggc aatatagata atataaatag tataaataat
      781 atttatattg tgatgcttgt cttgggtaaa aagagggatt tatggttaaa aagactttaa
      841 gtgtaactgt agatgaaaag gttcttgaaa aatggaaaaa atatactgat gaggaatgta
      901 ttaactcttc tcagcttatt gaaaaattac ttaaagaaca cttgaagaaa aggggggaga
      961 aatgaaaaga aggtttttaa ttggattaat ttctttggta tttatccttt ttttaattgg
     1021 atttgtttct acttgggtag ataatggaaa ttcaagtcag agtgtagatg gatgtaatca
     1081 attaaacaca actggagcta cttacattct aacacaaaat gtttctagta acggaacttg
     1141 tttcacaata acccgaccaa atattacatt agattgtaat gggtattgga taaattattc
     1201 tataaatggt ttagaaaaca catacggagt ttatacaaat caacttaaca caactataaa
     1261 gaattgtaat attgttgatg ggaattggac aggtgaagct gctggagata ggagaggtat
     1321 ttatcttaag aatgtaaatg gttcaactat ttttaataat tacataaggg ttaataattc
     1381 tgctgcaata atgttggaag gagaatcaag tttcaataat gtaacaaata atattgctta
     1441 tggtggttta tatggaatag acatacaagg acataataat attatttcca acaactttgc
     1501 aaattcttca tgagtttatt attcatattc ttctgctatg attatttatg gaggacaaaa
     1561 caacacttta ctagacaatg ctctttatgg aagatattat ggattatcta tttcaaactt
     1621 tgcaaataat acttttggaa aaaatctttc aattattgca gaggaacatt caggaataac
     1681 tgtggagttt ggggataata atactttaac tgatttttca gtaattagta gaggaaatta
     1741 tggaattaat cttgcattaa gtgcaaataa tacatttaag gattgtacaa taactaatac
     1801 aggatggtat gatgttaatg taggatggga ccctggatgt attaataata ctcttgtaaa
     1861 ctgttcttat tctacagaac gggttccaac ttctgatgga gaattaatac gtaagtggta
     1921 ttatcaagcg tatgtaaacc atactaatgg aaatccttta tcaagtgtaa atatctcagc
     1981 ttacaataca acaggacaat tgcaattcac aacattaaca aattcttctg gttggatacc
     2041 tagacaggaa gttgtagaat acattggctc aaatcaaaca agaagatatt acagcaatta
     2101 cacaataaat gcaacttcat tagggtatga agcagatagt aatacaatag attttacaac
     2161 aactcaaaat aaacttgatg atgtgtttac attacaagga cagggattgc aatgtggaga
     2221 attaaattca gcaaacacgg tttatacttt agcacaaaat gtttcatctc aaggaacttg
     2281 tttcacaata actcagccaa atattatatt ggactgtaat gggtattgga taaattattc
     2341 tataaatggt tcaaataata catatggaat ttattcaaat cagtttaata caactataaa
     2401 gaattgtaat attgttgaag gaaatttttc aaatccagta aataataaac acggtattta
     2461 tctttatgga gcagataatt caactatttt taataattat ataaatatcc aaagttctca
     2521 tccaattgat gttcgtggtt attctaaata cacaaatata acaaaaaata ctgtaataag
     2581 taattctgga ccagttgttt atttattaga tggagcagta aatagtgtag tagaaaataa
     2641 tacagttatc tcaaatggtt ctgcaggtat atttttatat ggtttatgta ataactgcgc
     2701 tgtaattaat aacaatgtat ctaatatagg agaaggaagt gaaggtatct atgtagtagg
     2761 gagtaatgat gtttttttag taaataatac agtaactggg agatatactt attcagttaa
     2821 tctatatcat tctctaaata cccttgtatt gaatcaagtt gcctatggtg gaaagagtgg
     2881 tttaaatatg tgggattcaa ataattctat aataaaagat tgtattaatt taagtggata
     2941 tgagtatgat gtacatatac cttctacata tttgggttca agtataaaca atactttcat
     3001 aaattgttct tatgattcaa ataaagagta tgttgatgga gaagaaaatg aactaataag
     3061 aaaatggtat tatcaagcac atgttaacaa cactgaaggt tctcctctct caaatgcaaa
     3121 tattgctgca tataattcat ctggacaatt acaattcaca actcaaacaa attcaagtgg
     3181 attaattgat agacaagaag ttacagaata tgttaataga ggtggaacaa gaagttacta
     3241 taataattac ataataaatg caacttcatt agggtatgaa acagatagca atatatttaa
     3301 cttcactaca acccaaaata aacttaatga tgtatttaca ttacaaggag aaggattaca
     3361 ttgtggagaa ctaaattctg caaatacagt ttacacatta gcacaaaatg tttcatctca
     3421 aggaacttgt ttcacaataa ctcaaccaaa tataac
//
LOCUS       DAOPCH010000066         3372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_22542_length_3372_cov_6.021706, whole genome shotgun sequence.
ACCESSION   DAOPCH010000066 DAOPCH010000000
VERSION     DAOPCH010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22542_length_3372_cov_6.021706"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLU55_04695"
     CDS             complement(<1..298)
                     /locus_tag="PLU55_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87389.1"
                     /translation="MLNKRVTIAYADLNASYAGDTIEVDPSMEFTVSPIAGEVTKTVY
                     PIGIQIINDTGAEIEYNAVTSVEEYNMYSDTNPPVLQRLQKSGTLTTDYTNFPKC"
     gene            complement(288..617)
                     /locus_tag="PLU55_04700"
     CDS             complement(288..617)
                     /locus_tag="PLU55_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87390.1"
                     /translation="MEELKKSLEELSENCTVKFIGLSLKLVELSKKVDSNYIENNNTI
                     LDIIKDFKDKIPDTTLFFQQMEKMQENFYYMTKEIIKINSRLYKLEKNMKKIDTNKQE
                     EEKRNVE"
     gene            complement(631..1077)
                     /locus_tag="PLU55_04705"
     CDS             complement(631..1077)
                     /locus_tag="PLU55_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87391.1"
                     /translation="MNKILFILFFILIIIKGCDILHAKEDNNIYIYQNKKVVGYFTKE
                     EFASLVNNSDLYVSEIEAEKEGRVEIKVIDSPYKIEKENKYKARFTITWKDSKDKVLK
                     EITLDSIITINEDASNPYPAWRVLYRNITEYSTPVLIILCIILAII"
     gene            complement(1191..1643)
                     /locus_tag="PLU55_04710"
     CDS             complement(1191..1643)
                     /locus_tag="PLU55_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87392.1"
                     /translation="MIKYNPNLFLNDHCFTLKEEREYKQFCQYVKLYESYGNRAFLLN
                     EFSFPKELMEKLSFVKDLAKATKFKIKDLFNLIKDKKVFKLFSYVKWNITNLAELIQK
                     GFQVYKDLQLVIADFIKKTKVMKFTTDKIQDLICAWNDYKNIFSIPRE"
     gene            complement(1640..1843)
                     /locus_tag="PLU55_04715"
     CDS             complement(1640..1843)
                     /locus_tag="PLU55_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87393.1"
                     /translation="MYIGVGAITLVVIIPILVYWFYLRTRGKTISFTPEFKVIDIKNS
                     EEIDMDVSKEAVKKGKDILKKIK"
     gene            complement(1878..2264)
                     /locus_tag="PLU55_04720"
     CDS             complement(1878..2264)
                     /locus_tag="PLU55_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87394.1"
                     /translation="MATALPVKLQLQITGNNIVSNDTYTKQYEVIAKSEKQFYNIKST
                     DGALPVSIEKLGEVVVITADASNAVLQIITSTPETIEIPIAGKLFWEIPSTFAGIITN
                     VNVRTDSTTNVNAEVSLWGVAPVVVS"
     gene            complement(2302..>3372)
                     /locus_tag="PLU55_04725"
     CDS             complement(2302..>3372)
                     /locus_tag="PLU55_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87395.1"
                     /translation="VGNIPSQIIEADTDIKDIPTDLIVDVPIDAQQRFKDNLIKSIKD
                     IIGKPTSSENKFIQNTDYVNNIFNNDTLTNVDGMLIADASDMPENLLWNQIKHKFFNK
                     DTTTDQDKWFIKRAVSERRFLGIDNAFSSRGDIMGLCVLHKEWSIKKNGIVYVIDFCF
                     GVGGFDSGINLEAPTYLVLDLINNGGVYIKDVASDTFHSEAQKQMLGRHNIELIKQSV
                     DTDVNPYQYMLTCLVNENLKAGRNIFLKNNLFSLMRTKGKNGKEKIDHNQGETEKVYN
                     GDWEKSKCGVFAKDVSDSVCQALWLAFQNEERPVTIYEEENKKFSTDETDVKYFKDKA
                     IQSVMLAQNTMIDIDKVKSSLT"
BASE COUNT         1032 a          551 c          419 g         1370 t
ORIGIN      
        1 aacactttgg aaaatttgta taatctgtag taagtgttcc acttttttgt agtctttgaa
       61 gaacaggggg gtttgtatca ctatacatat tgtattcttc tacagaagta acagcattat
      121 attctatttc agcaccggta tcatttataa tctgaatgcc tataggataa acagtttttg
      181 taacttctcc tgcaatggga ctaacagtaa attccattga agggtcaact tcaatagtat
      241 cccctgcata tgaggcattc aaatcggcat aagctattgt tacacgttta ttcaacattt
      301 cttttttcct cttcttgttt attagtatca atttttttca tatttttttc gagtttatat
      361 agtcgggaat ttattttgat tatttctttt gtcatataat aaaagttctc ttgcattttt
      421 tccatctgtt gaaagaataa tgtagtatca ggtattttgt ctttgaagtc tttaattata
      481 tctaaaatag tattgttgtt ttcaatgtag ttgctatcaa ctttttttga aagttccacc
      541 aattttagac ttaaaccaat aaacttaact gtacagtttt cagataattc ttccaaagac
      601 ttttttaatt cttccatttt tttccttcct ttaaattatt gcaagaatga tacacaatat
      661 aatcaatact ggtgttgaat actctgttat atttcgatat aatacacgcc atgccggata
      721 aggatttgaa gcatcttcat tgattgttat tatactgtct aaagttattt cttttaaaac
      781 cttatccttt gagtccttcc atgttattgt aaatcttgct ttatacttat tttctttttc
      841 tattttgtaa ggggagtcta taactttaat ttcaactcta ccttcttttt cagcctctat
      901 ttcagagaca tataaatctg aattgtttac aagggaagca aattcttctt ttgtgaaata
      961 tccaacaact tttttatttt ggtaaatata tatattgtta tcttctttag catgaagaat
     1021 atcacaacct tttattatta ttaaaataaa aaataaaata aataatattt tgttcatttt
     1081 gtccttccaa aaatttttaa gaaaattctt cactgtcatc tcctttagtt aatttaactt
     1141 taacattttt tgcaagtgtg gttataactg ctattgtaaa tttaattaaa ttactccctg
     1201 ggtatggaaa agatattttt gtaatcattc caagcgcaaa taaggtcttg tatcttatca
     1261 gtagtaaatt tcatcacttt tgttttcttt atgaaatctg ctattactaa ttgaaggtct
     1321 ttatatactt gaaagccttt ttgaattaac tctgcaaggt tagtaatatt ccattttaca
     1381 taagaaaaaa gtttaaatac ttttttatct tttataagat taaataggtc tttaatctta
     1441 aattttgtgg cttttgcaag gtctttaaca aaagaaagtt tttccattaa ttctttcgga
     1501 aaagaaaact catttaaaag aaatgcacga ttgccataac tttcataaag tttaacatat
     1561 tgacaaaatt gtttatactc tctttcttct ttaagagtaa agcaatggtc atttaaaaat
     1621 aaatttggat tgtatttaat cattttattt tttttaaaat atccttccct ttttttacag
     1681 cttctttgga aacatccata tcaatctctt cagagttttt aatatctatt accttaaatt
     1741 caggagtgaa agaaatagtt ttccctcttg ttctcaaata gaaccaataa acaagtatag
     1801 ggattattac cactaaggta atagccccca cacctatata cattaaaatt tttttaattt
     1861 tttctttatt tattttttta agaaacaaca actggtgcaa ccccccataa tgatacttct
     1921 gcattaacat ttgtagtact gtctgttctt acatttacat tagtaattat tcctgcaaat
     1981 gtagaaggaa tttcccaaaa caactttcct gcaatcggaa tttcaatagt ttctggtgta
     2041 cttgtgatta tttgaagtac tgcatttgag gcatcagcag taataacaac cacttcacct
     2101 aatttttcaa tacttacggg aagtgcccca tctgtacttt ttatgttgta aaactgtttt
     2161 tctgattttg caattacttc atactgtttt gtataagtat cattagaaac tatattgttt
     2221 cctgtaattt gcaattgtaa tttaactgga agtgctgttg ccatattttt ttgacctctt
     2281 ttctattatt agtacccaaa atcaggtcaa actgctttta accttgtcaa tatctatcat
     2341 tgtattctgt gcaagcatta ctgactgtat agctttatcc ttaaagtatt taacatctgt
     2401 ttcatctgtt gaaaacttct tattttcctc ttcataaatt gtaacaggtc tttcctcgtt
     2461 ttgaaatgct aaccaaagag cttgacaaac tgagtctgaa acgtctttag caaatacacc
     2521 acatttactt ttttcccaat caccattata tactttttct gtttcaccct gattatggtc
     2581 tattttttct ttaccgtttt ttccttttgt tcgcattaaa gaaaataaat tattttttaa
     2641 gaatatattt cttcctgctt ttaaattttc attaactaaa caagttagca tatattgata
     2701 aggattaaca tcggtgtcca ctgattgttt tatgagttca atattatgtc taccaagcat
     2761 ttgtttttgt gcttcactgt ggaaggtatc tgaagccaca tctttaatat aaacaccacc
     2821 attatttatt aaatccaaaa ctaaataagt aggtgcttca agattaatac ctgaatcaaa
     2881 accaccaaca ccaaaacaaa aatctatgac atagacaata ccattctttt taatgctcca
     2941 ttctttatga aggacacata agcccattat atcccctcgg ctactgaaag cattatctat
     3001 acctaagaaa cgtctttcac taacagctct ttttataaac catttgtctt ggtctgttgt
     3061 tgtgtcttta ttaaagaatt tatgtttaat ctgattccac aataaatttt caggcatatc
     3121 tgaagcatca gcaattaaca tgccatcaac atttgttaga gtatcattgt taaatatatt
     3181 atttacatag tctgtatttt gaataaattt gttttccgaa gatgttggtt ttccgattat
     3241 gtctttaatc gacttaatta aattgtcttt gaatctttgc tgtgcatcaa taggaacatc
     3301 aactattaaa tcagtaggga tgtcctttat gtctgtatct gcttctatta tttgagaagg
     3361 gatattacca ac
//
LOCUS       DAOPCH010000067         3343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_22776_length_3343_cov_5.943431, whole genome shotgun sequence.
ACCESSION   DAOPCH010000067 DAOPCH010000000
VERSION     DAOPCH010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22776_length_3343_cov_5.943431"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLU55_04730"
     CDS             complement(<1..223)
                     /locus_tag="PLU55_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87396.1"
                     /translation="MSVFRIDYTIDDNYHTKAEKNYILWEAEKVSDIDYVLVRQATKG
                     NSRDVYKFLHVVELNDVELLLEKLNNKFIH"
     gene            complement(258..782)
                     /locus_tag="PLU55_04735"
     CDS             complement(258..782)
                     /locus_tag="PLU55_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87397.1"
                     /translation="MKKTLTKYKRLSEADLTGRSIIDTKHAIDRFRDRYNNRYSIGRI
                     RKVVYDAMSTIINKYHDEATTYGVWSKSTGICVIIDWRRDTMNSYDNLNHAVIVTLPP
                     VKGEFKNFHTVNPKDVRFIVERCLYEEVVEKISLKESFKPNNLKEVKINNHSVFLHEG
                     KMFDSGISFCIEVE"
     gene            complement(788..1102)
                     /locus_tag="PLU55_04740"
     CDS             complement(788..1102)
                     /locus_tag="PLU55_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87398.1"
                     /translation="MKTLTKYKRLEEKTSKEFDEKDLKRLQKKYNDAFTFSFTNFKGG
                     DYDINIYWFNDKTDIISPSFSPYDGNRTEHPIYYNLNRQGARRYKTIKDIIDFRAKQF
                     LR"
     gene            complement(1153..1695)
                     /locus_tag="PLU55_04745"
     CDS             complement(1153..1695)
                     /locus_tag="PLU55_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87399.1"
                     /translation="MEKLKHYKSLDEYFKPILNYRGHTVADIDHSIERFKERMPNVNY
                     FVYERLLKKAINWLYNNKREGIEDRYIFCSKQHGFGIQLEWRRDRHNPKVLNGYTATT
                     LSNNEMKYFKFDDKKIFVENFVKAGYSLEESTNIANKGYGRFDFEGELKEELKSIHYN
                     LYVDEGSIRKDFELVTLIEE"
     gene            complement(1839..2003)
                     /locus_tag="PLU55_04750"
     CDS             complement(1839..2003)
                     /locus_tag="PLU55_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87400.1"
                     /translation="MLIHTVKDLIEELNKLDKDMPIRTIKNKKYSHVDVVIDAVYDPE
                     EEFINIVVVH"
     gene            complement(2021..2752)
                     /locus_tag="PLU55_04755"
     CDS             complement(2021..2752)
                     /locus_tag="PLU55_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87401.1"
                     /translation="MFENIEMVRKNSVYFKTDGNTVAHKVNSFYPISSILNDPEIKDL
                     YLQHEDGNGYRLLVEVNNVLYDMKYVCLYSKEDIEKYIKIGNVVVNAEYVYNYYKNRK
                     MGDFFNLVELEMVEAFNDEELLAFAKESRALCIKNRELRDIERKREQEEAQQKRIEEN
                     NNKVVALEEAIRNDKDIEIAWINGKVNLFYLFEKYNINVPIKVKGWISERLIKIYADG
                     RFSYRKSCSNSFWNYYSELKIAIKK"
     gene            complement(2812..3111)
                     /locus_tag="PLU55_04760"
     CDS             complement(2812..3111)
                     /locus_tag="PLU55_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87402.1"
                     /translation="MKKITRATFKSFVKKNSDKLLAKVISDFNGMTDCVEHLSDAEYS
                     PARKAEIDSEENNLGFNEIWLVGDSRDYFKLIDNEKYFGYHVSNCCGSFDIVIAK"
BASE COUNT         1052 a          581 c          338 g         1372 t
ORIGIN      
        1 tatgtatgaa cttgttattt aatttttcca acaataattc aacatcattc aattccacaa
       61 catgtaaaaa tttatataca tctctactat taccttttgt tgcttgcctc accagtacat
      121 agtcaatatc cgaaactttt tcagcttccc ataatatata attcttctct gctttagtgt
      181 ggtaattatc atctattgta taatcgattc tgaatacact catttttgtt ctccttttta
      241 aaaactttct ttcaagatta ttcaacctct atacagaaac taataccact gtcaaacatt
      301 tttccttcat gcaaaaatac agaatgatta ttgattttaa cctctttaag attgttaggt
      361 ttaaaggatt ctttcaaact gatcttttca acaacctctt catataaaca tctttcaaca
      421 ataaaccgaa cgtctttcgg attaacagta tgaaagtttt taaattcacc tttaacaggt
      481 ggtaatgtaa ctataacagc atgattaaga ttatcataac tattcatagt atctcttctc
      541 caatcaatta taacacatat accggtagat ttagaccata ccccataggt agtagcttca
      601 tcatgatatt tgtttatgat tgtagacata gcatcatata caacttttct tattcttcca
      661 atactatatc tattattata tctatctctg aatctatcaa tagcgtgttt ggtgtctata
      721 atacttctgc ctgttaagtc agcttcagat agacgtttat attttgttaa agtttttttc
      781 atagtgttta tcttaaaaat tgttttgctc tgaaatctat aatatcttta attgtcttat
      841 accttcttgc cccttgacgg ttaagattgt aatagatagg gtgttctgtt ctattaccat
      901 catatggact aaaactaggg gatataatat cggttttatc attaaaccaa tatatattaa
      961 tatcataatc tcctccttta aaatttgtaa aagagaaagt aaaagcatca ttatattttt
     1021 tttgcaatct ctttaaatct ttttcatcaa attcttttga agttttttct tcaagtcttt
     1081 tgtattttgt taaagttttc ataaatattt acctctaaac tattagtgtt cttttaaact
     1141 attagtgttc tttcactcct caattaaagt aactaattca aaatcttttc ttatgcttcc
     1201 ttcatcaaca tacaaattgt aatgtatact ctttaactct tctttcaatt ctccttcaaa
     1261 atcaaatcga ccatatcctt tgtttgcaat gtttgtactt tcttctaagc tatatcctgc
     1321 ttttacaaag ttttcaacaa atattttctt atcatcaaat ttgaaatatt tcatttcatt
     1381 gtttgataga gtggttgccg tgtagccatt taatacttta ggattatgtc tatctcttct
     1441 ccattcaagc tgtatcccga atccatgttg tttggaacag aatatatatc tatcttctat
     1501 accttctctt ttattattgt acaaccagtt aattgctttt ttaagaagcc gttcataaac
     1561 aaaataattc acattaggca ttctttcttt aaatctttct atactgtgat ctatatcagc
     1621 tacagtatgc cctctgtaat ttaatatagg tttgaaatat tcatctaagc ttttataatg
     1681 ttttaatttt tccattcttg caacctttta tttattagtg tttatttact tactatattg
     1741 ttattcatac ttgtttactc ctttttctta acttatgtaa acagtataaa ccttgattct
     1801 tatttgtcaa caactttttt taaaaaaaag aaaaaaaatt aatgaacaac tactatgttt
     1861 ataaactctt cttcagggtc ataaacagca tctataacaa catcaacatg gctatacttt
     1921 ttatttttta tggtgcgtat aggcatatct ttatccagtt tattaagttc ttctattagg
     1981 tctttaactg tgtgtattaa catagtattc actccttata ttacttttta attgctattt
     2041 taagttcaga ataatagttc cagaaagaat tactacaact ttttctataa gaaaatctac
     2101 catcagcata tatcttaata agtctttcac ttatccaacc tttaacttta atagggacat
     2161 tgatattata tttctcaaaa agataaaaca gattgacctt accattaatc caagcaattt
     2221 caatatcttt atcatttctt attgcttctt ctaatgctac aactttgtta ttattttctt
     2281 ctattctttt ttgttgtgct tcttcctgtt ctctttttct ttctatatct ctaagttctc
     2341 tatttttaat acacagggct cttgactctt tagcaaaagc aagtagttct tcatcattaa
     2401 aagcttctac catttcaagt tctacaagat tgaagaaatc ccccatcttt cgatttttat
     2461 aatagttata aacatattca gcattgacaa caacatttcc tattttgata tatttttcaa
     2521 tatcttcttt tgaataaaga cagacatact tcatatcata gagtacatta ttcacttcaa
     2581 ccaacaatct atatccatta ccatcttcat gctgaagata aaggtctttt atttcaggat
     2641 cattaagtat agaagatatt ggataaaatg aattaacttt gtgtgctact gtattaccat
     2701 cagttttaaa atatacactg ttttttctta ccatttcaat gttttcaaac ataattattt
     2761 actccttata attttttatc accaagagcg gaatagtctc cgctcttttt tttactttgc
     2821 aatcactata tcaaaagaac cacagcaatt agaaacatga tacccaaaat acttttcatt
     2881 atcaataagt ttgaaatagt ctctgctgtc ccctacaagc catatttcat taaaaccaag
     2941 attgttttcc tcagaatcaa tttctgcttt tctagcagga gaatactcag catcagaaag
     3001 gtgctccaca caatctgtca taccattaaa atctgatata accttagcga gaagcttatc
     3061 actattcttt ttaacaaatg atttgaatgt tgctcttgta atttttttca tttctttatt
     3121 tactccttca ttaacttata taatgaatat atatctggtc ttatattttg tcaacaaaaa
     3181 aacttctaaa aaataaaaaa aaatatgaat gttagggtta actaacattt atatataaat
     3241 atgacaaata gtgtgatttt gtttattttt tttcatattt cgggaaacat gaaaataaat
     3301 atagattgca tactgttatg tttactttaa tttaactatt cct
//
LOCUS       DAOPCH010000068         3316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_22991_length_3316_cov_21.744250, whole genome shotgun
            sequence.
ACCESSION   DAOPCH010000068 DAOPCH010000000
VERSION     DAOPCH010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22991_length_3316_cov_21.744250"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3303)
                     /locus_tag="PLU55_04765"
     CDS             complement(<1..3303)
                     /locus_tag="PLU55_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HPJ87403.1"
                     /translation="MTLNEQLQRMKSIMSDVERQSIPAIKRELLDLSKSFGLSIEELG
                     NSMYEILSSNFNPEFAQKLLAGATKMATAGLSTLEEATSLLISSINAYGYSVDSINTL
                     SDAFFEAVKVGRFTIGELGEDFATASTTAAMFGIDIREVLTALATMTNQGIKTNEAIT
                     ALNRLMMNFASGGSQEAKRVAKEFGIEMNSNAIKAKGLAGLVRDLNKATEEQIIALTG
                     NVRAFKAMASVMTDPERYEKFYKEISNAAGATERALKEVEESQAFRLKKYKQEFTVGM
                     MDIAELVMPLLIEGQKLLVSISKLFGGMGAKMALTTVAAGILAIAISKLIAKYNALSA
                     SIAGSNTQLAAASVAMKAASKIAAPVAIVAGIASIAAAIWQLASNAKKAKKEMDELNK
                     SIDETVKEYETMYAAEEVEAKQKFETNIMHIREYVRIAKDETKTQERRKLALESVKKE
                     MKALGELYPAEFEKLDLEQLKLENNLVYWDKYEIAIENTTKALQRHIDKLKAFAKVAG
                     YQATISKAQEAVNDLDVEIKMLFSDFDKEYKKLQEYYKIQGFYQGSKTISGTDLYNKL
                     QRYFELTTKLNISPFTYTPPPKPPTVGYSKEPKELYDLYQDVMPIIQYYQGGPYLDNK
                     LYKAIKQLYDLEKQKADLEKKLLQATSLMETEGKRILEEEANAQLDYLMKQRPSIPTG
                     GGEIAETGTTEKYDYRAALKIIEDAYLKSYETVSVGLDYDKIRELERAKEQELFDQAV
                     EIINKIEKPTERDEAMLDFVKWLYDNRKERLTLILSNLKKKMDEASTAEVQSAIQKQM
                     NDTINELLIAATQYQGFSLEEWKKKGATYKLPTTAGFQFGENKELQKILSEEDINIIK
                     NFSMSPSTYKFYEKLVNDLKEQNKNYNLKKLIEDWIKAKNDNDIATQERISLEIMQIA
                     NNEKKFKLDLLQLSELLNSEAGLTAKELEELNTRIDLLGSGLWAAQLEYIVEAKTNVE
                     TTMERLDREYKSYNKKNIEIIKEWINSRLLSIEEIRKEANVLNTKFENLTIKEIDNLL
                     LKPLPEDVKSVLDDRKNELGVSYKLLEQAHQEDLRILKTTQQKYYEDIDNLFEGKGTA
                     KIFSIGVMKLLYM"
BASE COUNT          921 a          677 c          434 g         1284 t
ORIGIN      
        1 catataaagt agcttcataa ctcctataga aaagattttt gcagttcctt taccttcaaa
       61 aagattgtct atatcttcat aatatttttg ttgagtagtt tttagtattc ttaaatcttc
      121 ctgatgagct tgttctaaaa gcttataaga gactcccaac tcattctttc tgtcatccaa
      181 tactgactta acatcttcag gtaagggttt aagcaataag ttgtctattt cctttatggt
      241 aagattttca aatttagtat taaggacatt ggcttctttt cttatctctt ctatagataa
      301 taaacgagag ttaatccatt cctttataat ctctatgttt ttcttgttat agctcttata
      361 ctctctatcc aatctctcca ttgtcgtttc tacattggtt tttgcttcta caatatattc
      421 caactgagct gcccataaac cagaacccaa taagtcaata cgagtattca attcctctaa
      481 ctcttttgca gttaagcctg cttctgaatt taacaattca gataactgca ataaatccaa
      541 cttaaacttc ttttcattat tggcaatttg cattatttcc aaacttatcc tttcctgagt
      601 tgctatatca ttatcattct tggcttttat ccaatcctct attaactttt ttaaattata
      661 atttttattt tgctctttta aatcattaac taacttttca tagaacttat aagtagaagg
      721 agacatacta aagttcttga tgatgtttat atcttcttca cttaatattt tctgcaattc
      781 tttgttttca ccaaactgaa aacctgctgt tgtaggcaat ttataagtag cacctttctt
      841 tttccattct tctaaactaa aaccttggta ttgagtagct gcaatcaata attcattgat
      901 agtatcattc atctgtttct gaatagctga ttgtacttct gctgtagaag cttcgtccat
      961 cttctttttt agattgctta agattaaagt caatctttct ttcctgttat catataacca
     1021 cttaacaaaa tccagcatag cttcatctcg ttcagttggt ttctctattt tattgataat
     1081 ctcaactgcc tgatcaaaga gttcctgctc tttagctctt tccaattccc gtatcttatc
     1141 ataatccaat cctacagaaa cagtttcata agatttaaga taggcatcct ctattatttt
     1201 caaagctgct ctataatcat acttttcagt agttccagtt tcagctatct ctccacctcc
     1261 agtaggaata cttggtcttt gtttcatcaa ataatctaat tgtgcattgg cttcttcttc
     1321 caatattctt tttccttcag tttccattaa agatgttgct tgcaataatt tcttttctaa
     1381 atctgctttc tgtttttcta aatcatataa ttgttttatg gctttataaa gcttgttatc
     1441 taaatacgga cctccttgat aatattgtat aatcggcata acatcttgat ataaatcata
     1501 tagctctttt ggttctttag aataaccaac tgtaggaggt ttaggtggtg gtgtataagt
     1561 aaaaggactt atgtttaact tggttgttaa ttcaaagtat ctctgtaatt tattatacaa
     1621 atctgttcca gaaatagttt tactaccttg ataaaaccct tggattttgt aatattcctg
     1681 aagtttctta tattccttgt caaaatccga gaagagcatt ttgatttcta catccaaatc
     1741 attaacagct tcttgagctt tggagatagt agcttgataa ccagctactt tggcaaatgc
     1801 ctttagttta tcaatgtgtc tctgcaatgc ttttgtagta ttctctattg ctatttcata
     1861 cttatcccaa taaactaaat tattttctaa ctttaactgt tctaaatcca acttttcaaa
     1921 ttctgcagga tacaactctc ctaatgcctt catttccttc tttacagatt ccaatgctaa
     1981 ctttcttcgt tcttgagttt tggtttcatc cttggcaatt ctgacatact ctcttatgtg
     2041 cataatattg gtttcaaatt tttgtttggc ttctacttct tctgcagcat acatcgtttc
     2101 atattctttt acagtttcat ctatactctt attcaattca tccatttctt ttttagcttt
     2161 cttggcatta cttgctaatt gccaaatagc agctgcaata ctggcaattc ctgctactat
     2221 ggctacagga gctgcaattt tagaagctgc cttcatagcc acagaagctg cagctaattg
     2281 agtattgctt cctgcaatag atgcacttaa ggcattatat ttggctatta acttactaat
     2341 tgctatggct aatataccag ctgcaactgt agttaatgcc atcttggctc ccataccacc
     2401 aaataactta cttatagaaa ctaataactt ctgtccttct atcaataatg gcattactaa
     2461 ttctgctata tccatcattc caacagtaaa ttcttgctta tacttcttca acctaaaagc
     2521 ttgactttct tctacctcct taagagctct ttctgttgca ccagctgcat tagaaatctc
     2581 tttatagaac ttctcatatc tttcaggatc agtcatcacc gaagccatag ctttgaaagc
     2641 tcggacattg ccagttaaag cgattatctg ttcctcagtt gctttattca aatctctaac
     2701 taatccagct aaaccttttg ctttaatggc attagagttc atctctatac caaactcttt
     2761 ggctactctc ttagcttcct gtgatcctcc actggcaaaa ttcatcatta acctattaag
     2821 agctgttatt gcttcattgg tctttattcc ctgattagtc attgtagcta aagctgttaa
     2881 gacctctctt atatcaatgc caaacatagc tgctgttgta gaagctgtag caaagtcctc
     2941 tcctaactca cctatggtaa atcttcctac tttaactgcc tcaaagaaag catctgataa
     3001 agtattgata ctgtcaaccg aatagccata agcattgata ctggaaataa gcaaagatgt
     3061 agcctcttct aaggtagaaa gacctgcagt tgccatcttg gttgcacctg ctaataactt
     3121 ttgcgcaaat tctggattga agttggaaga cagtatctca tacatagaat tgcctaactc
     3181 ttctatactt aagccaaaac ttttagataa atccaataac tctctcttaa tggcaggtat
     3241 ggattgtctt tctacatctg acataatgga tttcattctt tgtaactgtt cattaagggt
     3301 catagctgca ttctta
//
LOCUS       DAOPCH010000069         3241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_23660_length_3241_cov_25.726616, whole genome shotgun
            sequence.
ACCESSION   DAOPCH010000069 DAOPCH010000000
VERSION     DAOPCH010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23660_length_3241_cov_25.726616"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3228)
                     /locus_tag="PLU55_04770"
     CDS             complement(<1..3228)
                     /locus_tag="PLU55_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HPJ87404.1"
                     /translation="MTLNEQLQRMKSIMTDIEKQSIPAIKDSIIDLAKTYGLSMEELG
                     SSMYEILSSNFNPEFATKILEGSTKMATAGLSTLQEAVSLMITSINAYGYSMDDITTL
                     SDAFFEAVKVGRFTIGELGEDFATAATTAAIFGIDIREVLTALATMTNQGIKTNEAIT
                     ALNRLMINFASGGSAEAKKIAKELGIEMNSNAIKAKGLAGLVRDLNGATEEQIVALTG
                     SVRAFKAMASVMADVDRYEKFSKTITNSMGATDKALAEIQESQSFRMKKYKQEFIANM
                     MNIADEIMPVLLKAQKLLTDISKVFGGWGAKVALSGVSALALTYILYKMVTAFRSLAA
                     ANAQANVQLGTFSLLTKTLGKAGVVGAIAAIASTAIATIWQLVETTKKAKKEMDILKI
                     DIEKSVNESVLAATASQKEAIAKYQFTIKLAREYVRIAKDETESTYKRKNALDQLKKT
                     MSDLAEEYPANFEKLDTEKLKLEDNVTYWNEYEKAINLTTAALEKHLRRMQDFAKLSG
                     YTNLITKYQEQINNLSEQETTLKEEYLSKLRSKGVIKKGTSLAEEEKYWSYITKFYNI
                     GGASNFLAYQYTNPPQPVPAGVPNPSQELIDIYYRYNPFGAVDAGKYFRQYKNIKINM
                     DKALKKQEDVTKLMDIFSTEMAERWAEKDILGELGKTPKPLPTGRGDTGGGKKPAYDY
                     QKKLKLLQDRYQRSYELLPATVDYKAIAEKERTKEQDFFQQAVDIIQGIEKEDDRDKA
                     TIDLVKWMYDEKKKRLTTIMSLLKKQMEKETSPAEQEKIKDQMADIVQEMLVNATQYQ
                     AFDLEEFKKNPDKYLLPKEAAFGFENKELIKLITEEDEKIIEDMQMSIPTYTLYQKII
                     ENRKKLTAKQGGKNAADITEEWVKATKAQDYVTADKLLLELQDIAKDEKDFKLDLITL
                     TEMLQSEKGLTKDQLAELGKRVDLLSSGLWASELEYLITAKGEIETTLDKYMREYTKE
                     QNKNLESVKLFLEENELITNWEDLTVQMIDDLLLLPLPEEVKSALEIRKKELQKSFEE
                     LDKAHQEELKELSRRQQQYYEDIDSLLAGKGTAKIFSIGVMKLLYM"
BASE COUNT          907 a          627 c          443 g         1264 t
ORIGIN      
        1 catataaagt agcttcataa ctcctataga aaagattttt gcagttcctt tacctgccaa
       61 gagactatct atatcttcat agtattgctg ttgtcttctg gatagttctt ttaactcttc
      121 ctgatgagct ttatctaact cttcaaaact cttttgtaat tccttctttc ttatttccaa
      181 tgcagactta acttcttctg gtaaaggcaa taatagtaaa tcgtctatca tttgaactgt
      241 caggtcttcc cagttagtaa taagttcatt ttcttctaaa aagagcttaa cactttccag
      301 attcttgttt tgttcttttg tgtattctcg catatattta tctaaagttg tttctatctc
      361 tcctttagcg gtaataagat actctaactc tgaagcccat aatccagaac ttaataaatc
      421 tactctctta cccaactctg caagttgatc tttagttaat cctttttcag attgaagcat
      481 ttctgttaaa gtaattaaat ctaacttaaa atctttttca tctttggcta tatcttgtaa
      541 ctccaataac aacttatctg cagttacata gtcttgtgcc ttagttgctt ttacccattc
      601 ttcagttata tctgcagcat tcttaccacc ttgtttggca gttaacttct ttctattttc
      661 tataatcttt tgataaaggg tatatgtagg aatactcatc tgcatatctt caattatctt
      721 ttcatcttct tctgtaataa gcttaataag ttctttgttt tcaaatccaa aagcagcttc
      781 tttaggtaat aaatatttat caggattttt cttaaactct tccaaatcaa atgcttgata
      841 ttgagtagca ttaactaaca tttcttgcac tatgtctgcc atctgatctt ttatcttttc
      901 ctgttctgca ggtgaggttt ctttttccat ttgtttctta agcaaagaca taatggtagt
      961 taatcttttt ttcttttcat catacatcca tttgaccaaa tcaatggtag ctttatctct
     1021 atcgtcttct ttttctatgc cttgaataat atctacagct tgttgaaaga aatcttgttc
     1081 tttggttctt tccttttctg caatagcctt ataatctact gtagcaggta atagttcata
     1141 agatctttga tatctatctt gtaataactt tagttttttc tgatagtcat aagcaggttt
     1201 ttttccacct ccagtatcac ctctacctgt aggtaaaggt ttaggagttt taccaagttc
     1261 tcccagaata tctttttcag cccatctttc agccatttct gtagaaaaaa tatccattag
     1321 ttttgtaaca tcttcttgtt tttttaatgc cttatccata ttgatcttaa tgtttttata
     1381 ttgtctaaag tattttcctg catctacagc tccaaaagga ttgtatctat aataaatatc
     1441 aataagttcc tgtgatggat ttggaactcc agcaggaact ggctgaggcg gatttgtata
     1501 ttgataggca agaaaattag aagcaccacc aatattataa aatttggtaa tataactcca
     1561 atatttttct tcttctgcta aagaagttcc ttttttaatt actcctttgc ttctcagttt
     1621 acttaaatat tcttctttta atgtagtctc ttgttcagat aagttattta tttgctcttg
     1681 atatttggtt attaaattgg tataaccgct taacttcgca aaatcctgca ttcttctaag
     1741 atgcttttcc aatgcagcag tagttagatt tatagctttc tcatactcat tccaatatgt
     1801 tacattatct tccaatttta gtttttctgt atccaatttt tcaaagttag caggatattc
     1861 ttcagcaaga tctgacatag ttttctttaa ctgatctaaa gcattctttc ttttataggt
     1921 agactctgtc tcatctttgg ctattctgac atattctctg gctaacttaa tggtaaactg
     1981 atatttggca atggcttctt tctgtgaagc agtagctgcc aatacacttt cgttaacact
     2041 cttttcaata tctattttta gaatatccat ttctttctta gctttcttgg ttgtttctac
     2101 aagttgccaa attgtagcaa tcgctgtaga tgctatagca gctattgcac ctactacacc
     2161 tgctttgcct aatgtcttag ttaataatga gaatgtgcct aattgaacat tggcttgagc
     2221 attggcagct gccaaacttc taaaagcagt aaccatttta taaaggatat aagtaagtgc
     2281 caaagcagat acacctgata aagctacttt agcaccccaa cctccaaata ccttagatat
     2341 atctgttaat agtttttgtg ctttaagtaa gacaggcata atctcatctg ctatattcat
     2401 catattggca ataaattcct gtttatactt cttcattcta aaagattgac tttcctgtat
     2461 ctctgctaag gctttatctg tagcacccat agaattggtt attgttttgg aaaacttctc
     2521 atatctatct acatcggcca ttacagatgc catagcctta aatgctctta cacttccagt
     2581 taaagcaact atctgttctt ctgttgcacc attaaggtct cttaccaatc ctgccaaacc
     2641 tttagcctta atagcattgg aattcatttc tatacctaac tctttagcta ttttcttagc
     2701 ttctgcagat cctcctgaag caaaatttat catcaatcta ttaagtgcag ttatagcttc
     2761 attggtctta ataccctgat tggtcatagt agctaaagca gttagaactt ctctaatatc
     2821 aataccaaag atagcagctg tagtagctgc agtagcaaaa tcttctccca attcacctat
     2881 ggtaaatctt cctactttaa ctgcctcaaa gaaagcatct gataaggtag ttatatcatc
     2941 catagaataa ccataagcat tgatagatgt tatcatcaat gatactgctt cttgtaaagt
     3001 agatagacct gcagtcgcca tcttagtaga accttctaaa atcttagtag caaattcggg
     3061 attgaaattg gaagatagaa tctcatacat agaagaacct aactcttcca tagataaacc
     3121 ataagttttg gctaagtcta taatactatc cttaatggca ggtatggatt gtttttctat
     3181 atctgtcata atggatttca ttctttgtaa ctgttcatta agggtcatag ctgcattctt
     3241 a
//
LOCUS       DAOPCH010000070         3087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_25109_length_3087_cov_3.260884, whole genome shotgun sequence.
ACCESSION   DAOPCH010000070 DAOPCH010000000
VERSION     DAOPCH010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25109_length_3087_cov_3.260884"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="PLU55_04775"
     CDS             <1..1402
                     /locus_tag="PLU55_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87405.1"
                     /translation="SFQVSEKGSMIKNKYEPSEYLQATLNISFFNQSLNSTFNDSLGN
                     SVSLSTLLSQSYNIEYSSIPFDSTNTSINSAPQLFYIAGTSFQMPATEGNITYRLDLD
                     GQKLFEKVLEISSRGKDIEKILASKYVDLNISKHEISEYDISTQKILNEYFKIKQIEE
                     KLNLIKSEYEKGSSSEYESLLENLTEIRIPKKITKTANVNPMAFYPDREAINLYILAQ
                     IDGGEFLGKENEYIDAVYIWNNEKLKTTVKFNEITAYYEAGNQEVVNVYTFNFDKSKM
                     KEEAYMIMGNLENVIFEDETLPVKEYNGYLYFNLNDVYDTVSFSTTSNINFLDIPVFI
                     SPSIDKLTPPKIGDYERWQKPKKWALFVLIVIILVLISVISYILLQIWYRKKYETYLF
                     KTKNNLYNIMVYIQGAKRKGMSRDDIIKNLKKAGWTKEQVNYALQKYEGKKIAGLIHP
                     PLNIGSQEQEKKPKKK"
     gene            1444..2085
                     /locus_tag="PLU55_04780"
     CDS             1444..2085
                     /locus_tag="PLU55_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Era-like GTP-binding protein"
                     /protein_id="HPJ87406.1"
                     /translation="MAIKIFKNFFKKIFNSLFKNKKEIKLGFYGPPNAGKTSLANRIC
                     KDFTGQEIGSVSKVPHETRNVQFKEKVEIKYKGKTLMFKMIDTPGIATKIDYEEFLKY
                     NMKKKEAKERAKEATQGVIESIKWLDDMDVVVIVLDSTENPYNQVNITIVGNLVARKI
                     PVLIVGNKIDLKKSDLKKIEAAFPEYDVVGVSARSGENFEEFYESIFKLAKNI"
     gene            2112..2519
                     /locus_tag="PLU55_04785"
     CDS             2112..2519
                     /locus_tag="PLU55_04785"
                     /inference="COORDINATES: protein motif:HMM:NF021373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2073 domain-containing protein"
                     /protein_id="HPJ87407.1"
                     /translation="MAKGDKKLGKIKGFSIQFLPYSEIRDLNSHERIRRILNIVLGNN
                     ILLIQGRLRSEEEARLIGDTMAMIGHVKDFKGIELAVIEGGGDMSILDRMRSSLIKAL
                     SKGDLGAITIIGPATIVKEIKRNPKKIELLLSK"
     gene            complement(2537..>3087)
                     /locus_tag="PLU55_04790"
     CDS             complement(2537..>3087)
                     /locus_tag="PLU55_04790"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPJ87408.1"
                     /translation="PKDKSLREIYDLQNTERVFFDIYNSKKEINQEFIINLHKGLMAN
                     IDLRTGYRTTDIRVIKSNFDATPGSYVKTDMDLLLKWYNKNKKNLHPLTLATVFHHKF
                     EKIHPFMDGNGRTGRMLFTYILLRNKYPPIIIHKKTRKDYLEALRKADKSGLLEADVN
                     NYFELVQFCADELIKGYWNLFL"
BASE COUNT         1253 a          368 c          488 g          978 t
ORIGIN      
        1 ttcatttcaa gtatcagaga aagggagtat gataaaaaac aaatatgaac cttcagaata
       61 tcttcaagca acattgaata taagcttttt taaccaatct ttgaactcta cattcaatga
      121 ttctttagga aatagtgttt cattatccac attattaagt caatcttaca atattgagta
      181 ttcttctata ccatttgatt caactaatac ttcaattaat tctgctcctc aattatttta
      241 tattgcagga acaagctttc agatgcctgc aacagaagga aatataactt acagattaga
      301 ccttgatggt caaaaattgt ttgaaaaagt gttggagatt agttctagag gaaaagatat
      361 agaaaagatt cttgctagta agtatgttga tttgaatatt tcaaaacatg aaataagcga
      421 atatgatatt tctacacaaa aaatattgaa tgaatatttc aaaataaaac aaattgaaga
      481 aaaattaaac ttaataaaat cagaatatga aaagggttct tcaagtgaat atgaaagttt
      541 attggaaaat ctaactgaaa taagaatacc aaaaaaaata actaaaacag ctaatgtaaa
      601 ccctatggca ttttatcctg acagagaagc aataaatctt tatattctag cacaaataga
      661 cggaggagaa tttttaggta aggaaaatga atatattgat gctgtttata tatggaacaa
      721 tgaaaaatta aagacaactg ttaaattcaa tgaaataact gcttattatg aggcaggtaa
      781 tcaagaagta gtaaatgttt atactttcaa ctttgacaaa tcaaagatga aagaggaagc
      841 ttatatgatt atggggaatt tagaaaatgt tatatttgaa gatgaaactc ttcctgttaa
      901 agaatataat ggatatcttt attttaattt aaatgatgtt tatgatacag taagcttctc
      961 aacaacttca aatatcaatt tcctagatat ccctgtgttt atctctcctt caatagataa
     1021 attaactcct cctaaaatag gagactatga aagatggcaa aaacctaaga aatgggctct
     1081 ttttgttctt atagttataa ttttagtttt aattagtgta atctcttata ttcttttaca
     1141 gatatggtat agaaaaaaat atgaaactta tcttttcaaa acaaagaaca acctttacaa
     1201 tataatggta tatattcaag gtgcaaaaag aaaaggaatg tctagagatg atataataaa
     1261 aaatcttaaa aaagcaggat ggacaaaaga acaagtaaat tatgcattgc agaagtatga
     1321 aggcaaaaaa attgctggac taatacatcc tccactcaat ataggttctc aagagcaaga
     1381 gaaaaaacct aaaaagaaat agtaacaaaa atttaataaa cctatctgaa taaaacatat
     1441 aatatggcaa taaaaatatt caagaatttt tttaaaaaga tatttaatag tctttttaag
     1501 aataaaaaag aaataaaatt aggattctat gggcctccaa atgcaggaaa aacgagttta
     1561 gctaacagaa tatgcaaaga ctttacagga caagaaatag gaagtgtgag taaagttcca
     1621 catgaaacaa gaaatgttca atttaaagag aaagttgaaa taaaatacaa gggtaaaact
     1681 ctaatgttta agatgattga tactccgggt attgctacaa aaatagatta tgaagaattc
     1741 ttgaagtata atatgaagaa aaaagaagct aaagagagag caaaagaagc tacacaaggg
     1801 gttattgaga gtataaagtg gttagatgat atggatgttg ttgtaatagt cctagattca
     1861 acagagaacc cttataatca agtaaatata actatagtgg gtaatttagt tgctagaaaa
     1921 atacctgtat taattgtagg taataaaata gatttaaaga aatctgactt aaaaaaaata
     1981 gaagcagctt tcccagaata tgatgtagtt ggagttagtg caagaagtgg tgagaacttt
     2041 gaagaatttt acgaatcaat atttaaattg gcaaaaaaca tttaaaaaat aattttacat
     2101 aacataataa gatggcaaag ggtgataaaa aactaggaaa gataaaggga ttttctatac
     2161 agtttcttcc ttattctgaa ataagggact taaatagtca tgaaaggata agaaggattc
     2221 tcaatatagt tttaggaaat aatatattgt taatacaagg gagacttagg tctgaagaag
     2281 aagcaagact tattggagac actatggcaa tgattggaca tgtaaaagat ttcaaaggta
     2341 ttgagcttgc agtaatagaa ggtggtggag atatgagtat tcttgataga atgagaagca
     2401 gcttaattaa agcattaagt aaaggagatt taggggcaat aacaataata ggacctgcaa
     2461 caatagttaa agagattaaa agaaatccaa agaagattga attgctttta agtaagtgaa
     2521 attattaaag aagatgttat agaaataaat tccagtatcc tttaattaat tcatcagcac
     2581 aaaactgcac taattcaaaa taattattaa catctgcctc taataatcca ctcttatcag
     2641 cttttcttaa tgcttccaaa taatctttcc ttgttttttt atgtataatt attggagggt
     2701 atttattcct gagtagaata taagtaaaaa gcattcttcc tgttcttcca ttcccgtcca
     2761 taaatggatg tattttctca aacttatgat gaaatactgt tgcaagagtc agaggatgta
     2821 aatttttctt attcttgtta taccatttta ataataaatc catatctgtt ttaacatagg
     2881 aaccaggagt tgcatcaaaa ttagatttta taactcgaat atctgttgtc ctatatcctg
     2941 ttcttaaatc tatatttgcc attaatcctt tatgaagatt aataataaat tcctgattaa
     3001 tctctttttt tgaattataa atatcaaaga atactctttc tgtattttgt aaatcataaa
     3061 tctctcttaa acttttatcc ttaggag
//
LOCUS       DAOPCH010000071         3062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_25344_length_3062_cov_27.952777, whole genome shotgun
            sequence.
ACCESSION   DAOPCH010000071 DAOPCH010000000
VERSION     DAOPCH010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25344_length_3062_cov_27.952777"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(292..1434)
                     /locus_tag="PLU55_04795"
     CDS             complement(292..1434)
                     /locus_tag="PLU55_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87409.1"
                     /translation="MSNPNKNTANTITSQVNDLNIKSLKKSFNVEAAITGLFVLVAVI
                     FSAFVASGFKFTWEVFYDEKFYIQTAINFAIMMFTYSFVKEIVIRRAKATNEEYQKVK
                     GKELEFVKFVRDNFLEEMIADKVAMANEERRLQAANNILARITYGLYAQDLENFEDPN
                     NISVNDDAFNDFCLKHGLITYKKDKATGKVKATVNSKKAKQLRKVIVRVLKGDYKYQP
                     IYTKEILTTTDADISSIDLYSYNEHKANIKEATRKGVLWLVASALTSSIYWAGWDLSF
                     WLTLLTNTTLVLSSALTAIMAANQRIKILTMVSENKTGFLNQATKDYRLELKNGKEEV
                     KEIVKEETNTQEKVAYSKEEDKVIQVSTIPKELPNIEYETTSTSAL"
     gene            complement(1427..1978)
                     /locus_tag="PLU55_04800"
     CDS             complement(1427..1978)
                     /locus_tag="PLU55_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87410.1"
                     /translation="MTQSQIRKKITKRDWKTYFKKRWLYLLGALFIYVAPLVIIFEAL
                     MRIEPNQTNISISFLGFVTGITYLVFFSKRLNAKIKELKTGALKTFLTGLISLIPFIT
                     VGFLAHLINNGFANFQHAIWGIIASMFIGVVCQTIDYLINADYLYGLELKKIAADKIF
                     AEQEEERLRTEIEALRGGGNNNE"
     gene            complement(1994..2656)
                     /locus_tag="PLU55_04805"
     CDS             complement(1994..2656)
                     /locus_tag="PLU55_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87411.1"
                     /translation="MKKTNILKIIWVFILMLFLVGGTCSYYVVKANGGEEPPQTEPET
                     QEEGEVVDPLPPITEQGFFTRLFGGFNDLLTNPQFNQIMNWVMVAIGFIIGIWAKIRS
                     GQLKVKATKAKYDFELLLAEKTKLTTENEAVKAVLAKAVKQADAMKKAMILAFDRSNL
                     KEDVKDKIKGYFDEVEQLEEIPIEKIVEELEEEKVVEPFTNKTAEFVNEEQKQEVKPL
                     EW"
     gene            complement(2671..2937)
                     /locus_tag="PLU55_04810"
     CDS             complement(2671..2937)
                     /locus_tag="PLU55_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87412.1"
                     /translation="MSNKDNQKVNNGVYVVQYKKGIATQSFIPLEEVIYKDQPLGKYL
                     ESLEKEILSLKKKLVNLNNVLAKQQEATKKSFDIVLEKVNDGVL"
BASE COUNT          946 a          524 c          334 g         1258 t
ORIGIN      
        1 tgtcaacata aatatataaa attatttatt tttttttaat atagtttata attatgttat
       61 gattattatg aaaggagtga taaaatgaat aattttaata agttttctaa taatattact
      121 tattttttac taaataaagt tattaagtca atgataaatc ataaaggcat aaaaaaaaag
      181 gaagatataa taatcttcct tttttaatgt taatatagag aaacacatat ttaataagcg
      241 ttaatgtgag ccgattatct tttaaagttg actaattctc aattaatctt tttatagagc
      301 gcttgtagaa gttgtttcat attctatgtt aggtaattct tttggaattg tgctaacttg
      361 tattacttta tcttcttcct ttgaatatgc aactttttct tgcgtattag tttcttcttt
      421 tacaatctct ttaacttctt ctttaccatt tttaagctct aacctataat ctttagttgc
      481 ttgatttaag aatccagttt tgttctcact taccattgtt aaaatcttta ttctttgatt
      541 agctgccata atagctgtta atgcacttga taatacaagt gttgtatttg ttagtaaagt
      601 aagccaaaat gatagatccc agccagccca ataaatactt gaagttaatg ctgatgctac
      661 aagccataaa acacctttgc gtgttgcttc ttttatatta gctttatgtt cgttatatga
      721 atataaatca atactgctaa tatcagcatc agtagttgtt aaaatctctt ttgtataaat
      781 tggttgatat ttataatcgc cctttaagac tcttacaata acttttctta attgttttgc
      841 tttcttggaa ttaacagtcg ctttcacttt accagtagct ttatcttttt tataagtaat
      901 tagtccatgt ttaagacaaa aatcattaaa agcatcatca ttaaccgaga tattatttgg
      961 atcttcaaag ttctctaaat cttgtgcgta aagtccataa gtaatacgag ctaaaatgtt
     1021 attagcagct tgtaatcttc tttcttcatt agccatagca actttatcag ctatcatttc
     1081 ttctaaaaag ttatctctta caaacttaac aaattcaagt tctttccctt taactttttg
     1141 atattcttca tttgttgctt tagctctacg aataactatt tctttaacaa atgaataagt
     1201 aaacatcata attgcaaagt taatagctgt ttgaatataa aacttttcat cataaaagac
     1261 ttcccatgta aacttaaaac cacttgctac aaaagctgaa aatattacgg ctactaatac
     1321 aaataagcca gttatagctg cttctacatt aaagctcttt tttaagcttt taatgtttag
     1381 atcattaact tgacttgtaa tagtattagc tgtgttttta tttggattac tcattattat
     1441 ttccaccacc tcttaaagct tctatttcag ttcttaacct ttcttcttct tgttccgcaa
     1501 atatcttatc agctgctatt ttctttagtt ctaaaccata taaataatca gcattgatta
     1561 aataatcgat tgtctggcat actacaccaa taaacattga tgctataatt ccccaaattg
     1621 catgttggaa gttagcaaaa ccattattga ttaaatgagc taaaaacccc actgtaataa
     1681 atggaattaa acttattaaa ccagttagaa aagtctttaa tgctccagtc tttaattctt
     1741 ttattttagc atttagtctt ttgctaaaaa atactaaata agtaattcca gttacaaaac
     1801 ccaaaaaact aattgatata ttcgtttggt ttggttctat tcgcattaaa gcttcaaata
     1861 tgataactaa aggtgcaaca taaataaata aagctcctaa aagatatagc caccttttct
     1921 taaaatatgt tttccaatct cgttttgtta tctttttcct aatttgcgat tgtgtcataa
     1981 ttaacaccta cctttaccat tctaatggtt ttacttcttg tttttgttct tcattaacaa
     2041 attcagctgt cttattagta aatggttcta ctactttttc ttcttctaat tcttctacaa
     2101 tcttttcaat tggtatttct tctaattgct ctacctcatc aaaataacct ttaatcttat
     2161 cttttacatc ttctttcaag ttagacctat caaatgctaa aatcattgct ttcttcatgg
     2221 catcagcttg tttaactgct tttgctaaca ctgccttaac tgcttcattt tcagttgtta
     2281 atttagtttt ttcagcaagt aataattcaa aatcgtattt agctttagtt gcttttactt
     2341 ttaattgacc gctcctaatt ttagcccaaa taccaataat aaaaccaatt gcaaccataa
     2401 cccaattcat tatttgatta aactgtggat tagttaataa atcattaaat ccaccaaata
     2461 accttgtaaa gaaaccttgc tctgttatgg gaggtaatgg atctaccact tccccctctt
     2521 cttgtgtttc tggttcggtt tgtggtggtt cttccccacc attagcttta actacataat
     2581 aactacaagt tccaccgacc aagaaaagca ttaaaataaa tacccaaata atttttaaaa
     2641 tgtttgtttt tttcatattt tactccttta ttataaaacc ccatcattta ctttttctaa
     2701 tacaatatca aagctctttt tagtagcttc ttgctgttta gctaaaacat tatttaagtt
     2761 aacaagtttt ttctttaagc ttaaaatctc tttctctaaa ctttctaaat actttcctaa
     2821 tggttggtct ttgtagataa cttcttctaa aggtataaag ctttgagtag caataccttt
     2881 tttgtattgc actacataaa ccccattatt gactttttga ttatctttat tactcataaa
     2941 taaccccctt ttgtttttta gctaatcgtt cttgctcttt tttatatttt ttcacaattc
     3001 ttacaacttt cttttcttgt cttttgcgtt ctcgttcttg tttttttaat aacctttttt
     3061 ct
//
LOCUS       DAOPCH010000072         3048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_25494_length_3048_cov_40.637487, whole genome shotgun
            sequence.
ACCESSION   DAOPCH010000072 DAOPCH010000000
VERSION     DAOPCH010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25494_length_3048_cov_40.637487"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLU55_04815"
     CDS             complement(<1..432)
                     /locus_tag="PLU55_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87413.1"
                     /translation="MIILLSWSSLRTDNKYDISYDGILKKEIQRLELRNQDRISEVVK
                     IYILPEHYLDILVLIKLESNGDSLAYFQGNYGLMQINQVHLNKAKKDSIYHLDFNLRL
                     GYELYKKARDEDYANRFACYNRSYLKYHYQQKAYKLLEDQNE"
     gene            complement(446..1228)
                     /locus_tag="PLU55_04820"
     CDS             complement(446..1228)
                     /locus_tag="PLU55_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87414.1"
                     /translation="MSWFDEEDAKIESGQFTGVHKCVIREAEHNVTDTGAESITLRVE
                     LEGKKDFNGNPKIISLETIYLTNGKQKTSFANRVSALFCLLLANPNKLKKYRVDRYNF
                     STKAKEPKIVEMYEELLNKNVALFIQLRRSYRLKKIDPATGYVVRTNEPGIWLPDYNK
                     DKKLSYQFVRAFDYKTIQTYSEITSKKPAKIYLELASKMSDYEEEKMGKADFLKYAQK
                     QADKAVLKLDESIIYSFFDEYNEIEDSDLPADLDLNSDKVPF"
     gene            complement(1240..1905)
                     /locus_tag="PLU55_04825"
     CDS             complement(1240..1905)
                     /locus_tag="PLU55_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013379580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPJ87415.1"
                     /translation="MAEMVAIMGETGHGKSHSIQYLNPEETFIFNCDKKSLPFKGWKS
                     KYSIEKQNYAFTTDPSEIIRLLKAISILNERKNIKYIVIDTLNAVMIDDEMKRMKDVG
                     YDKWVDLAVSVYGILDTAACLRDDLTIFCMFHTQDLLDDVGNHFYRVQTSGQKLQKIK
                     LESKFPVVLHSKCDYGDEKNEYYFETQANHSTAKSPEGMFPAKRIPNNLKLVADYVTE
                     YNS"
     gene            complement(1898..2302)
                     /locus_tag="PLU55_04830"
     CDS             complement(1898..2302)
                     /locus_tag="PLU55_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87416.1"
                     /translation="MRDKEIFVQIIDTFITALENNKKVDWKDIPMPELLNEEEVDNAL
                     IVFDNSNTDLNENYPNHDNIADELIESLFTPEDCDLMFYNISLLQTIETFKSRKALYN
                     NIELQKTLLILIAMTYLTIEKYKSSQKEVNNG"
     gene            complement(2299..2697)
                     /locus_tag="PLU55_04835"
     CDS             complement(2299..2697)
                     /locus_tag="PLU55_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87417.1"
                     /translation="MLYHEIEDLINKAKKSTEGVLKAVYEIGRWWLANSELLGSEIGD
                     YELKQTNYGDKIIYTYNNRVLGMGYDGNLGNFRYGSQDNHIRFLEKFPIALKEKLISI
                     INDNQKCLDSFDDIVKFILNEQEKLVKVKK"
     gene            complement(2699..3019)
                     /locus_tag="PLU55_04840"
     CDS             complement(2699..3019)
                     /locus_tag="PLU55_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87418.1"
                     /translation="MADQVKQPVETCKVPDLVLYNLIEQYQELLEEEKRLSKFKDEKK
                     QKILELIGDAETVEVGKFVVNNKTIVTTSLDVKAVKQEAPDIYNSFLKVTESKRLTIK
                     EKEI"
BASE COUNT          868 a          562 c          431 g         1187 t
ORIGIN      
        1 ttcattttgg tcttccaata gtttataggc tttttgttga taatgatatt tgagataaga
       61 cctgttgtaa caggcaaacc tgttagcata gtcttcatcc cttgctttct tatacaattc
      121 ataaccaagt ctcaaattga aatccaggtg atagatacta tctttcttgg ctttatttaa
      181 gtgaacttgg ttaatttgca ttaaaccata attgccttga aagtaagcta aactatcacc
      241 attagattcc aattttatca agacaaggat atctaagtaa tgttctggta atatatagat
      301 cttaacaacc tcagatatcc tgtcttgatt tcttaattcc aatctttgta tttccttttt
      361 tagtattcca tcatagctta tatcatattt gttatcggtt cttaaagaac tccaagataa
      421 caatataatc aagctaaggg atattttaga aaggaacttt atcactattc aaatccagat
      481 ctgcaggtag atcactatct tcaatttcat tatattcatc aaagaaacta taaatgatac
      541 tttcatccag cttcagaact gctttatctg cttgtttttg agcatattta aggaaatctg
      601 cctttcccat tttttcttct tcataatcag acatcttgct tgccaattct aaatagatct
      661 tagcaggttt cttgctggtt atctccgaat aagtctgtat agttttataa tcaaatgctc
      721 taacaaactg ataagataat ttcttatctt tattataatc tggtaaccag ataccaggtt
      781 cattggttct gacaacataa cctgtagcgg gatctatctt ctttagcctg taacttcttc
      841 gtaactgaat aaagagagct acattcttat ttaacagttc ttcatacatt tctactatct
      901 taggttcttt agctttggtg ctaaagttat atctgtcaac tctgtatttc tttagcttat
      961 taggattggc taacaataga cagaataaag cagataccct atttgcaaac gaggtctttt
     1021 gtttaccatt ggttagatag atagtctcta aactgataat cttcggatta ccattgaaat
     1081 cttttttacc ttccaattct actcttaaag tgatgctttc tgcacctgta tctgtaacat
     1141 tgtgctcagc ctctctaata acacacttat ggacacctgt aaattgtcca ctttcaattt
     1201 ttgcatcttc ttcgtcaaac caactcatct ttttcctcct tatgagttat attcggtaac
     1261 atagtctgct accaatttga ggttgttagg tattctttta gcaggaaaca ttccttcagg
     1321 agatttagcc gtagaatgat tggcttgagt ttcaaaataa tattcatttt tctcatctcc
     1381 ataatcacat ttggaatgta aaactacagg aaacttagac tccagtttta ttttctgcag
     1441 tttttgtcca gaagtttgaa ctctgtagaa atgatttcct acatcatcca acaggtcttg
     1501 agtatgaaac atacaaaaga tggttagatc gtctcttaag caggcagctg tatccagaat
     1561 accataaaca gaaacagcta aatctaccca cttatcataa ccaacatctt tcattcgttt
     1621 catttcatcg tctatcatta cagcattaag agtatcaata actatgtatt tgatattctt
     1681 acgctcattt aggatagaga tggctttcaa taacctgata atttcacttg gatctgtagt
     1741 aaaggcatag ttttgttttt ctatgctata cttacttttc caacccttaa acggaagaga
     1801 cttcttatca caattaaaga taaaggtctc ttcgggattg agatactgga tggaatgaga
     1861 cttaccgtga cctgtctcac ccataatagc taccatttca gccattgtta acctcctttt
     1921 ggcttgattt atatttctct atggtaagat atgtcattgc tataagaata agcagagttt
     1981 tttgtaactc tatattatta tataatgctt tacggctttt gaatgtttct atggtttgca
     2041 gcagagatat attgtagaac ataaggtcac aatcttctgg agtgaataga gattctatca
     2101 attcatctgc tatgttatcg tgatttggat agttttcgtt tagatctgtg ttactattgt
     2161 caaatacgat cagagcatta tcaacttctt cttcatttaa cagttctggc attggtatat
     2221 ctttccaatc aactttttta ttattctcta aagcagtgat gaatgtatct attatttgaa
     2281 caaagatttc tttatctctc atttttttac cttaaccagt ttttcttgtt cattcaatat
     2341 aaacttaact atatcatcaa aactgtctaa gcatttttga ttatcattga ttatgcttat
     2401 cagtttttct ttcaatgcta tagggaactt ttccaagaat ctgatatggt tatcttggct
     2461 tccatatctg aaatttccta agttaccgtc gtatcccata cctaaaactc tattgttata
     2521 ggtataaata attttatccc cataattagt ttgctttagt tcgtagtcgc caatttctga
     2581 gcctaataat tcagaattgg ctaaccacca tctaccgatt tcatatacgg cttttagaac
     2641 tccttcggta gatttttttg ccttatttat aaggtcttca atttcgtgat ataacatatt
     2701 aaatctcctt ttctttaata gttagtcttt tactttccgt aacctttaag aagctgttat
     2761 aaatatctgg tgcttcttgt tttacggctt taacatctaa actggtagtt actatcgtct
     2821 tgttattgac aacgaactta ccaacttcta cagtttccgc atctccaata agttctaaga
     2881 ttttttgttt cttctcatct ttgaacttag agaggcgttt ctcttcttct aatagttctt
     2941 gatattgctc tattagatta tagagaacta gatcgggaac tttacaagtt tccacaggtt
     3001 gtttaacttg gtctgccatt atttacctcc tttcttttta ttttgttg
//
LOCUS       DAOPCH010000073         3005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_25949_length_3005_cov_34.435593, whole genome shotgun
            sequence.
ACCESSION   DAOPCH010000073 DAOPCH010000000
VERSION     DAOPCH010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25949_length_3005_cov_34.435593"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..763
                     /locus_tag="PLU55_04845"
     CDS             113..763
                     /locus_tag="PLU55_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87419.1"
                     /translation="MPYNQLLGMQGCSVFRVIHANDGSIVDGWIPVYIHHTSPAISFH
                     PIYPPAENWRGQSFDMGGGYTIKMDLDIYNIQEEYTNILALAECINLIRTVPFTYQLK
                     IRPWYSTALIRKVNGVDTDFNFYFLGVLNGDISIDQVKKLLNTAQKITLSFTSEDIRP
                     KFLEYDIDFLNPGNHSDKPSEILEDGESWVTGREHEDIGDSRMPNDEPDCPYNKQE"
     gene            783..1484
                     /locus_tag="PLU55_04850"
     CDS             783..1484
                     /locus_tag="PLU55_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87420.1"
                     /translation="MTRYNALNITKIWQDKLNLQDFMLNLIGVRFPESKKYRFTDLIL
                     VIINIAGKVYYYEFIASTKPYLDNIFKADKIGYQGMLLPGYYPDFWKVESRYNNKLIQ
                     AYEVPVAIASLDVTSSKEKLYKLGRYGIVKGIDLRLCQSRRNAHNIGEVMSQCLAKND
                     DMLQLMGLAGLQYKQTGKKFVSYYLAKETNGYPVIPENNWRDAKVANSSPYAMIIKEG
                     NYKEGDSYYNNEEDN"
     gene            1468..1935
                     /locus_tag="PLU55_04855"
     CDS             1468..1935
                     /locus_tag="PLU55_04855"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013468512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPJ87421.1"
                     /translation="MKKITNIIVHCSDSEFGSASMIRQWHLKNGWKDIGYHFVIDNGL
                     LVPKAGNWQTELRLNSLDGSIEVGRYLDGDSFISDNEVGAHTLGYNATSLGVCLIGVK
                     SFTVNQFNSLTYLLKELLYYFKLKKDCIKGHYQVSKGKTCPNFDVPKFIKERL"
     gene            1932..2192
                     /locus_tag="PLU55_04860"
     CDS             1932..2192
                     /locus_tag="PLU55_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87422.1"
                     /translation="MMFKKIGLNASSLRHYAGTNIIVNMFMLVNAGFVYGAIVAMAMQ
                     ILVEFNQATGSSLGLREYLKTKWKDTLWDTLFAIAGILVILL"
BASE COUNT         1072 a          377 c          556 g         1000 t
ORIGIN      
        1 gaacctgaca ctgatgattt atattttata gtaacatcta taaaatggaa tataatggaa
       61 ctggaacttg aaggttttag agtttccagt taggatttaa caggaggtta aaatgcctta
      121 taatcaatta ttaggaatgc aaggttgttc agtattcaga gtaatacacg ctaatgatgg
      181 ttctatagtt gatggttgga tacctgtata tattcatcac acctcccctg ctatcagctt
      241 tcatcctata tatccacctg cagaaaactg gagaggtcaa tcttttgata tgggaggtgg
      301 ttatactatc aagatggatt tggatatcta taatattcaa gaagaatata caaacatttt
      361 agcattagca gaatgtatta acttaatcag aacagttcct tttacctatc aactgaagat
      421 tagaccttgg tatagcactg ctcttattag aaaagtaaat ggtgtagata cagattttaa
      481 tttttacttc ttaggtgttc ttaatggaga tatttctata gaccaggtaa agaagttatt
      541 aaatactgct caaaagataa ctttatcctt taccagtgaa gatattagac ctaagttttt
      601 agaatatgat atagattttc taaatccagg taaccatagt gataaaccca gtgagatact
      661 tgaagatggc gaatcttggg ttacaggtag ggaacacgaa gatattggcg atagcagaat
      721 gccaaatgat gaaccagatt gtccctataa caaacaagaa taaaataaaa aggagaaaaa
      781 aaatgaccag atacaatgct cttaatataa ctaagatttg gcaagataaa ctaaatctgc
      841 aggattttat gttaaatctg ataggtgtca ggtttcctga aagcaaaaaa tacaggttta
      901 cagacctgat tttagtaatt atcaatattg caggcaaagt ttattattat gagtttattg
      961 cttctaccaa gccttatttg gataatatat tcaaagctga taagataggt tatcaaggaa
     1021 tgttacttcc aggttattat ccagatttct ggaaggtaga aagcagatat aataataaac
     1081 taattcaagc ttatgaagtc cctgtagcta tagcttcttt agatgttacc agtagcaaag
     1141 aaaagctata taaactgggg agatatggta tagttaaggg gatagattta aggttatgtc
     1201 aaagtagaag gaatgctcat aacattggag aggttatgtc tcaatgttta gctaaaaatg
     1261 atgatatgct gcaacttatg ggattagcag gattacaata taaacaaaca ggaaagaagt
     1321 ttgtaagtta ttacttggca aaagaaacga atggttatcc tgttatccca gagaataatt
     1381 ggagagatgc aaaggttgca aattcaagtc cttatgctat gataattaaa gaaggaaatt
     1441 ataaagaagg agatagttat tataacaatg aagaagataa ctaatatcat agtgcattgt
     1501 tcagactcag agtttgggag tgcttctatg attaggcaat ggcacttaaa gaatggttgg
     1561 aaagatatag gctatcattt cgtaattgac aatggtctgc ttgttcccaa agctggtaat
     1621 tggcaaacag agttaagatt aaactcttta gatggtagta tagaagtagg cagatatttg
     1681 gatggagata gttttataag tgataatgaa gtaggagctc atactttggg gtataatgca
     1741 acttcgttag gtgtatgttt aataggagtt aagagtttta ctgttaatca gtttaacagt
     1801 ttaacttatc ttttaaaaga gctgttatat tatttcaagt tgaaaaaaga ctgtattaag
     1861 ggacactatc aagttagtaa gggtaagact tgtcctaact ttgatgttcc caagtttatt
     1921 aaagagaggt tatgatgttc aagaaaatag gattaaatgc aagcagctta aggcattatg
     1981 caggaactaa cataattgta aatatgttta tgttggtaaa tgcaggcttt gtttatggtg
     2041 ctattgtagc tatggcaatg cagattttag tagagtttaa tcaggctaca ggaagttctt
     2101 taggattaag agaatatctt aaaactaaat ggaaagatac cctttgggat accctgtttg
     2161 ctattgcagg tattcttgtt atactgttat aagtatcggg ggaagagaaa acgggacagg
     2221 ttttttcctt gtctttttcc ctgtccctct ctttcccgta aataacacaa aatcgttact
     2281 tttaatttta aggcactttt taagcatttt gagagaggtt atcctcaaaa tggagtaatc
     2341 atactgctta atatgagaaa gttaataaga agcccctcaa atttcgttta atggcatttt
     2401 aatatgtcta catccattcc tttttcccaa ttccgtttca ggaacacctt gtaacataat
     2461 ataagttatt atattaacat aaattgagta tgattataag ggtctttcct attattctgt
     2521 tacatcctgt tacatcctgt tacatattta ttttacaaaa tgcataaatt ctatcaaaga
     2581 aatttaagcc aattttaagg gtgttaaatt aaaaaggcat aaattatact aaccaacata
     2641 tagaagttca ttagcgttga tatttgaccc cttaaaatca attttgagga tacagaggta
     2701 ttcttttttt cagaaatttt agattgacaa tgaaaaagtc aatcatttta ggttgtcaag
     2761 attcaaaaaa agagaaagaa aagaaaagta acataaaaga aaagaaagag aaagaggaat
     2821 atattagatc ttagatctta tatatcttaa agattatata tatattatat ataatatata
     2881 tcttagatat cttagatctt agagatcttt tatattataa aaagatcttt ctacttcata
     2941 gttttattta taagactaat ttattagggt tataaataag tttatgaaga atgagattta
     3001 gactt
//
LOCUS       DAOPCH010000074         2902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_27086_length_2902_cov_3.767475, whole genome shotgun sequence.
ACCESSION   DAOPCH010000074 DAOPCH010000000
VERSION     DAOPCH010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27086_length_2902_cov_3.767475"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..411)
                     /locus_tag="PLU55_04865"
     CDS             complement(10..411)
                     /locus_tag="PLU55_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87423.1"
                     /translation="MAGLTTHLIIVFVFGASIWIFSKRWYYAAAFGLGHLIPDLISFG
                     ITGIRQKSVNPGIIMTNDWFSPLATFSHNALNWAAILLVLWLGFVLLYSFKKIDKKQF
                     AGYILVLIYFIFGVILHLIVDKLIIEHNYWI"
     gene            complement(534..1319)
                     /locus_tag="PLU55_04870"
     CDS             complement(534..1319)
                     /locus_tag="PLU55_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87424.1"
                     /translation="MKKGGANFNFKRLSLFVIFIFGFTVAVSFVSANSGYVCINCPQN
                     QILKTTEKCKDFNENSNKFEIGDNICLNGKNFQAGRYNWEIKKDGVVVANGNKRVDNT
                     GKFCLETKTKECGEYEINFGGVFKAYSIECGEELPECTSQTVVGGTIYQGNIINGIEG
                     AEVWVTCNGETKHTTSIENGVYSIYFDCEVCGYGDLVSVSATYDGLTGENSGKIDMNF
                     ETSLGTELNVGIVNVPLVPEFGAIVATLTALGALGVFFVVRKK"
     gene            complement(1412..2119)
                     /locus_tag="PLU55_04875"
     CDS             complement(1412..2119)
                     /locus_tag="PLU55_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87425.1"
                     /translation="MKSVERNFIIALVGLAIVEYLLSSFPTIGFFVYAVFVGLILILM
                     ENEVKHTKEEKLLIILMILPICRIAEIFLNLEFFWSTLVFYLFIIGVTLYYVIKFWLR
                     FEKTPFIGNPTYFFGALGISGIAWAVSKYLFNWEFSGIVFLIPLIAYAEEIYFRGTFQ
                     NLANEWFGNFSILLTAFVYNIFSISYGFTYVLIAFSAALILSSLYHITKNIYISYVLN
                     IIFHVLLFMFYPVVYGI"
     gene            complement(2116..2310)
                     /locus_tag="PLU55_04880"
     CDS             complement(2116..2310)
                     /locus_tag="PLU55_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87426.1"
                     /translation="MITLSKIGEVSPIFLTVLLIIYLMIVELGSRRTRNALMPLVIIL
                     AIAFMILAVISVYTTYVNLK"
     gene            complement(2374..>2902)
                     /locus_tag="PLU55_04885"
     CDS             complement(2374..>2902)
                     /locus_tag="PLU55_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87427.1"
                     /translation="IIEGVGNSDNSYSYGIVVESNWADNTPTPADNIYIYNNVVTAAK
                     NTYAGVLISAGGDISNLNIRNNIIKGGFSYPIRINDRKLTTRINGLNINNNMFHGTSS
                     SSIYYSSGLTYSNRNDNGNLANVDPLFVGGSPYNFRLSSSSPARGKGIYVGLDRDYAG
                     QSWNNPPSIGAYEYK"
BASE COUNT         1105 a          564 c          333 g          900 t
ORIGIN      
        1 ctcttaaact catatccaat aattatgttc aattattagt ttatcaacaa ttaaatgcaa
       61 aataacccca aaaataaaat aaatcagaac caaaatataa cctgcaaact gctttttatc
      121 tattttctta aaactataca gaagaacaaa tcctaaccaa agcacaagta agatagcagc
      181 ccaattcaaa gcattatggc tgaatgttgc aagaggtgaa aaccaatcat tagtcattat
      241 aatacctgga tttacagatt tttgcctaat tcctgttatt ccaaaactta tcaagtcagg
      301 aattaaatgc ccaagtccaa aagctgcagc ataataccat cttttagaaa atatccaaat
      361 agatgctcca aacacaaaga caataattaa atgagttgtt aaccctgcca taacaatact
      421 tattttagaa ggtttttaaa tcttaattat acaaaaatct cccaaaagtt aatcaatcaa
      481 acagggaaaa ataaaaataa attcacaaaa aataaaaaaa caaattatct ggcttatttc
      541 tttctaacaa caaagaacac tcctaaagca cctaatgctg ttagtgtagc aacaatagct
      601 ccaaattcag gaactaatgg aacattaaca atacccacat taagttcagt tcccaaagat
      661 gtctcaaaat tcatatctat cttacctgaa ttctctccag ttaaaccatc atatgttgca
      721 cttacactaa ctaaatctcc ataaccacac acctcgcaat caaaataaat agaataaact
      781 ccattctcaa tgcttgtagt atgctttgtt tctccattac atgtcaccca aacctctgca
      841 ccttcaattc cattaattat attaccttga tagattgtcc caccaacaac tgtctgagat
      901 gtacattcag gaagttcttc tccacactca attgaatacg ccttaaatac tcccccaaaa
      961 ttaatttcat actctccgca ttcttttgtc tttgtttcaa ggcagaactt acctgtgttg
     1021 tctacacgct tatttccatt tgcaacaacc actccatctt tctttatttc ccaattatac
     1081 cttccagcct gaaaattttt accatttaaa caaatattat ctccaatttc aaatttgttt
     1141 gagttctcat tgaagtcttt acatttttct gttgttttta aaatttgatt ctgtggacaa
     1201 ttgatacaaa catatccaga attagctgaa acaaatgaaa cagcgacggt aaaaccaaaa
     1261 ataaatataa caaagagtga tagccttttg aaattaaaat tagctccccc ttttttcatg
     1321 ctatttacac acatgattta tttataaaca ttattatatt ttgcaatata tttccgtcgg
     1381 gaaaccctga tttttcaatc cttttcaaaa cttaaattcc atacaccaca ggatagaaca
     1441 taaacaacag aacatggaaa ataatattta ggacatagct tatgtagatg tttttggtta
     1501 tgtgatacaa gctacttaaa atcaaagcag cagaaaatgc aatcaaaaca taagtaaatc
     1561 catagcttat gctgaaaata ttatagacaa atgcagttaa aagaatagag aaatttccaa
     1621 accactcatt agccagattc tggaaagtcc ctctaaagta aatttcttct gcataagcaa
     1681 tcagaggtat taagaatact atcccagaaa attcccaatt aaataaatat ttagatactg
     1741 cccatgcaat tccagatatg cccaaagccc cgaaaaagta agtagggttt ccaataaaag
     1801 gagtcttctc aaatctcagc cagaatttaa ttacataata gagtgtaaca cctatgataa
     1861 acaggtaaaa cacaagtgtg ctccagaaaa attccagatt aaggaaaatt tcagctatcc
     1921 tacatatagg taaaatcatt aaaataatca aaagcttttc ctcttttgtg tgcttaactt
     1981 cattttccat taaaataagt attaatccaa caaaaacagc ataaacaaaa aaccctattg
     2041 taggaaaaga actaagtaaa tactccacaa tagccaaacc tactaaagca attataaaat
     2101 ttctttcaac tgatttcatt ttaaattaac atatgttgta taaacactta tcacagcaag
     2161 aatcatgaat gcaattgcaa gaataataac aagaggcatc aaagcatttc ttgttctcct
     2221 gcttccaagt tccactatca taagataaat tatgagcaat acagtaagga aaatagggga
     2281 aacctctcct attttagata aggttatcat agaaagtgtt attgtaataa ttatttaaat
     2341 gttactattc tatgataata ctataaaaat tttttactta tattcataag ctcctattga
     2401 aggaggatta ttccagctct gcccagcata atctctatcc aacccaacat aaattccttt
     2461 acctcttgca ggagaagaag aacttaaacg gaaattatat ggagaacctc ctacaaacaa
     2521 agggtcaaca ttagccagat ttccattatc attgcgatta ctataagtca aaccactaga
     2581 ataatagata cttgaacttg aagtcccatg aaacatatta ttattgatat tcaagccatt
     2641 tattctagta gtcaattttc tgtcatttat tcttatagga taactaaaac cccctttaat
     2701 gatattattt cttatattga gattagaaat atctcctcca gcagaaataa gaacaccagc
     2761 ataagtattt tttgctgctg taacaacatt attgtaaatg taaatattat ctgcaggagt
     2821 aggagtatta tctgcccaat tagattcaac aactatccca taactataag aattatctga
     2881 attacccact ccttcaatta ta
//
LOCUS       DAOPCH010000075         2885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_27265_length_2885_cov_4.552650, whole genome shotgun sequence.
ACCESSION   DAOPCH010000075 DAOPCH010000000
VERSION     DAOPCH010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27265_length_2885_cov_4.552650"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PLU55_04890"
     CDS             complement(<1..1076)
                     /locus_tag="PLU55_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPJ87428.1"
                     /translation="MEICTIGGYEEVGKNMTAVKVDDDVIIFDAGIYLPPLVELQEQE
                     VQASYTENMLRAAKAIPNDLVLDKLGWTDKVRAIVIGHAHLDHVGALPYLANRYPKAQ
                     ILASPFTMAVLEGILKDEKISMRNKRKIVNENSTYQIKGSSGNLKLEFIHTTHSTIQS
                     LFIAWHSKEGVFFYGLDFKFDNHPVIGAPPNYKRLKELGKSGVKCLVVDALYSDTDRR
                     TASERIARNLLEDALGNIHDKKSAIFVTTFSSHIARLKSIVEFGQKTGRKIFFLGRSL
                     NKYVEAAIKVNQCPFKNKIEIIKYRRQINSFLKRVESNREKYLIVCTGHQGEKGSILD
                     RVVHGQTPFKFRTGDNLIFSSSIIP"
     gene            1173..2618
                     /gene="amrB"
                     /locus_tag="PLU55_04895"
     CDS             1173..2618
                     /gene="amrB"
                     /locus_tag="PLU55_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPJ87429.1"
                     /translation="MKKKRFNLKLTVFFIVILIIGLIVLFIVTNKNKISQEEVREAKF
                     AGTWYPANKENLEKSIQEYFNNSKRIDFKGRVKAVIVPHAGYVYSGNIAASAFNQLQG
                     DYKTVFLMGPSHNYYLKNVSVSGFKYYSTPLGKVKVSKKAKEMDEKEEIINNIEEAHE
                     KEHSLELEIPFLQVKLKEFEIVPLLVGETNSTELKEVLLKYLDEEDLIVVSVDLSHYH
                     KYDEALILDSYSLNKILELDSKGIFNAEIDAPWAVAGLLEIAKEKSWKPYLVSYTNSG
                     EINGDKSSVVGYSAVVFIESFSEEEKEFLLSLARESAEKYLKDKTKLKINPEKVPENL
                     RVEKGCFVTFTKDEQLRGCIGYIIPQKPLYECVIENSINAAVNDPRFSPMKYNELKEA
                     RIDISVLSVPTLFKHSNYQELLSELENKVYGVVLKRGLHQSTFLPSVWEFIPEKENFL
                     GNLCLKGEMEAECWKDNRTEVYLYTAEDFSE"
     gene            2665..>2885
                     /locus_tag="PLU55_04900"
     CDS             2665..>2885
                     /locus_tag="PLU55_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87430.1"
                     /translation="MKALIASHKENKRYLVVKGEGKYLKENIEKAILEFSGKLGMSKC
                     GLSFIKSDKDKIIISVNREAVDLVRASIAV"
BASE COUNT         1017 a          381 c          479 g         1008 t
ORIGIN      
        1 ggtataatac tagaagaaaa aataagatta tctcctgttc taaatttaaa tggggtttgt
       61 ccatgaacaa ctctatccaa tatagaacct ttttctcctt gatgtcctgt acatacaatc
      121 aaatattttt ctctgttaga ttctactctt tttaaaaaag aatttatttg tctcctatat
      181 ttgattattt ctattttatt tttgaatggg cattgattta cttttattgc tgcttcaaca
      241 tatttgttaa gacttcttcc taagaagaat atttttctgc ctgttttttg tccaaactct
      301 acaatgcttt ttaatcttgc aatatgggag gagaaagttg taacaaatat agcagatttt
      361 ttatcatgga tgtttcctaa tgcatcttca agtaaatttc ttgctattct ctcactagca
      421 gttcttctgt ctgtatctga atataatgcg tctactacaa gacattttac tcctgatttt
      481 cctaattctt ttaatctttt gtaatttgga ggagctccaa tcacaggatg attatcaaac
      541 ttaaaatcca atccataaaa gaaaactcct tctttagaat gccatgcaat aaaaagagat
      601 tgtattgttg agtgagttgt atgtatgaat tcaagcttaa gatttcctga actcccttta
      661 atctgataag ttgagttttc atttacaatt tttcgtttat ttctcatgct tatcttttca
      721 tctttcaata ttccttctaa aacagccatt gtaaatgggc tcgcgagtat ttgtgcttta
      781 ggatatctat ttgcgagata aggtaatgct ccaacatggt caagatgagc gtgtcctatt
      841 actattgctc ttactttatc tgtccatcct aatttatcaa gaaccaaatc atttggaatt
      901 gcttttgctg ctctcaacat attctctgtg taactagctt gcacttcttg ttcctgtaat
      961 tcaactaatg gaggaagata aattcctgca tcaaatataa ttacatcatc atcaacctta
     1021 acagcagtca tgttttttcc aacttcttca tagccgccaa ttgtacaaat ttccattaca
     1081 gcaaatcctt ctctttttgt ataattaatt agcataattg ctttaaatat gtttttagga
     1141 acatttttag atgagagaat attaatttag agatgaaaaa aaagagattt aatttgaaat
     1201 taactgtatt ttttatagta atccttataa ttgggttaat tgtcttattt atagtgacta
     1261 ataaaaataa aataagccaa gaagaagtaa gggaagctaa atttgctgga acatggtatc
     1321 ctgcaaataa agagaatctt gaaaaatcaa ttcaagaata ttttaataat tctaaaagaa
     1381 tagattttaa gggaagggta aaagcagtta ttgtccctca tgcaggatat gtgtattctg
     1441 ggaatattgc tgcaagtgca tttaatcaat tgcaaggaga ttataaaaca gttttcttaa
     1501 tgggaccaag ccataattat tatctgaaaa atgtttctgt ttctggtttt aaatattatt
     1561 caaccccatt aggaaaagta aaggttagta aaaaggcaaa agagatggat gagaaagaag
     1621 aaataataaa taacattgaa gaagctcatg aaaaagaaca ctccttagaa cttgaaattc
     1681 cttttttgca agttaaactt aaagaatttg agattgttcc tttattggtt ggagagacaa
     1741 atagcacaga attaaaagaa gtgcttttga aatatttaga tgaagaagac ttaattgttg
     1801 tgagtgtaga cctttcacat tatcacaaat atgatgaagc gttgatactt gattcttatt
     1861 ctttaaataa aatactggaa ttagattcaa aagggatatt taatgcagaa atagatgctc
     1921 catgggcagt tgcaggttta ttagaaattg caaaagaaaa atcttggaaa ccatatcttg
     1981 tttcatacac taattcagga gagataaatg gagataaatc aagtgttgta ggatattctg
     2041 cagttgtttt tattgagagt tttagtgaag aagaaaaaga atttttgctt tctctggcta
     2101 gagagagtgc tgaaaaatat cttaaagata aaacaaaact caaaataaat ccagaaaagg
     2161 ttcctgaaaa tttaagagtt gaaaaagggt gttttgttac atttactaaa gatgaacagt
     2221 taagaggatg tatcggatat ataatccctc aaaaaccatt atatgaatgt gtaattgaaa
     2281 attcaataaa tgctgctgta aatgacccca gattttctcc tatgaaatat aatgaattaa
     2341 aagaagctag aattgatatt agtgttttaa gtgttccgac tctgtttaag catagtaatt
     2401 atcaggaatt actttcagaa ttagaaaaca aagtatatgg agttgtttta aaaagaggat
     2461 tacatcaaag cacttttctt ccaagtgtat gggaatttat acctgaaaag gaaaattttt
     2521 taggtaattt gtgtctgaaa ggagagatgg aagctgaatg ttggaaagat aatagaacag
     2581 aagtttatct ttacacagca gaggatttct ctgaataaaa ctttatacaa taacaaatat
     2641 aaactatttt atccttaata aattatgaaa gcactaatag catctcataa agaaaacaaa
     2701 aggtatcttg ttgttaaggg agagggtaaa tacttaaaag aaaatattga aaaggcaatt
     2761 ctggaatttt caggaaagtt aggaatgagt aagtgtggtt taagttttat taaatcagat
     2821 aaagataaaa taattatctc agttaatagg gaagcagttg atttagttag ggcgagtatt
     2881 gctgt
//
LOCUS       DAOPCH010000076         2858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_27625_length_2858_cov_2.846236, whole genome shotgun sequence.
ACCESSION   DAOPCH010000076 DAOPCH010000000
VERSION     DAOPCH010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27625_length_2858_cov_2.846236"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLU55_04905"
     CDS             <1..354
                     /locus_tag="PLU55_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPJ87431.1"
                     /translation="DEYIFPFLKNDALYAKLVTSEDFQKASPEQLANLFQSIESQIVR
                     YNALLKSIAFRAKIKKNLTSHIARHSFADIARKKVSVYDIQKMLGHSSVKVTEGYLKS
                     LDIEAMDEAMEQVFN"
     gene            444..1211
                     /locus_tag="PLU55_04910"
     CDS             444..1211
                     /locus_tag="PLU55_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87432.1"
                     /translation="MDFEKILPDGRRLRHTSPMYYVDWGRDKISNVWEAILQMNIPSP
                     TNETIQLQALLDEYSMASLMGDFLCMAYEFAGRKKVSQNYKAREKDYEDYELEHLKAI
                     KFLLDTRTEDILIDFRNKLYSKSLTLNNAKIITFIKEAMLDNFFRIKHELPSKRGKEY
                     WSNAIEQNIAELEKVKSKSGRKRKYHFLGSHIYTLRRYLQDNTDLKAREGILISRRQS
                     LFIFNFLSIYGFIGDDITSKEDNIYHILEDYVKGFDM"
     gene            1337..1612
                     /locus_tag="PLU55_04915"
     CDS             1337..1612
                     /locus_tag="PLU55_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPJ87433.1"
                     /translation="MEQFAIIPAERLDALQANLDEIKSLINQKNLQADFNRWHSKQEA
                     KIKLNVCMKTLDNYLAKGILPYSRFAGKIYIKNADIEAHLERNYITK"
     gene            1632..2087
                     /locus_tag="PLU55_04920"
     CDS             1632..2087
                     /locus_tag="PLU55_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87434.1"
                     /translation="MNERFIETSHIPLTYVKKDGTKGSFYLSNGKKGILPQRISLGIF
                     AQKKESQKDKLKKDEINGTYKKTEEGLYKSLNNGRVHSSIWGFEEYPEFYGYGIIDER
                     YALYDLVIMYSGNNCSSSFDVHLFRGMGKPDFIQKAFHYLRCYIKQKPQ"
     gene            2084..>2858
                     /locus_tag="PLU55_04925"
     CDS             2084..>2858
                     /locus_tag="PLU55_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87435.1"
                     /translation="MKGLQGYSMNLDYIDISKFLDKDRICLDYLQRNAIYDGAEMYYF
                     NGCRKIEVWMRQATNQVNIKGSIPYFINGHNFYASHQDWLEGLDYIQGCLNVNIYSGQ
                     VNSFEFGTIQEIPFPEPDFLNHHIKVRGMEARAYHKGQVLTGREFVSPSLKIKLYDVQ
                     RNIKTKLDKPIRADLSRDYGWDSRKHYIKLENHYRQPEAYFKGNVQLSELLSSSFQQD
                     LKKGLLTSYQSIMKTGTTAIPKSKADINAGTIPLMILKEL"
BASE COUNT         1001 a          508 c          608 g          741 t
ORIGIN      
        1 gatgaatata tcttcccctt ccttaaaaat gatgcccttt atgccaagct agtcacatca
       61 gaagacttcc agaaggcatc ccctgagcag ttagccaatc tgtttcagag catagaaagc
      121 cagattgtaa ggtacaatgc ccttctgaaa tccatagcat tcagggcgaa gattaagaag
      181 aacctcacat ctcacattgc aaggcattcc tttgctgaca ttgccagaaa gaaagtatct
      241 gtttatgaca ttcagaagat gttggggcat tcatcagtca aggtgacaga aggctatctg
      301 aaaagcttgg acattgaggc aatggatgag gcaatggaac aagtcttcaa ttaagggata
      361 ggggatgatg aaaatatttt gcaccctagc agaaaccaca gccctagctt atttaacaaa
      421 aatcttccca aaatactgca aagatggatt ttgaaaagat attgccagat ggaagaaggc
      481 ttagacatac aagccccatg tattatgtag actggggcag ggataagatt tccaatgtat
      541 gggaagctat tcttcaaatg aacatccctt cacctacaaa tgaaaccatt caacttcaag
      601 ccctattgga tgaatatagt atggcatctt taatggggga tttcctttgc atggcttatg
      661 aatttgcagg aagaaagaaa gtaagtcaga attataaggc aagggaaaag gattatgaag
      721 attatgaatt ggaacatctt aaagccatca agtttctact agatacaaga actgaagata
      781 tattgattga tttcaggaat aaactatata gcaagtcatt gacattgaat aatgccaaaa
      841 tcataacctt tatcaaagaa gcaatgctgg ataatttttt taggattaaa catgaactgc
      901 caagcaaaag ggggaaagaa tattggagca atgctattga gcagaatatt gctgaactag
      961 agaaagtcaa aagcaaatca ggaaggaaaa ggaaatatca ttttttagga agtcacattt
     1021 atacattaag aagatacctt caagataata ctgatttaaa ggctagggaa ggaattttga
     1081 tatccagaag gcaatcctta ttcatcttca attttttaag tatttatggc tttatagggg
     1141 atgacattac ctcaaaagaa gacaatattt accatatcct agaagactat gtgaaaggct
     1201 ttgatatgta aaacattcat ttgcagtaag aaaaaatatc ctagcaaaaa gccctattaa
     1261 tacttactta agtttctgat ttactggcta tttgattata tttttacaaa gaaatatcaa
     1321 tcaaaaaaag gtaaatatgg aacaatttgc aattatccca gcagaaaggc tggatgcctt
     1381 acaggcaaat cttgatgaaa tcaagtccct catcaatcag aagaaccttc aggctgattt
     1441 taatagatgg cattccaagc aggaagccaa aatcaagctg aatgtctgca tgaaaaccct
     1501 ggacaattat ctggcaaagg gtatccttcc ttattccaga tttgcaggga agatttacat
     1561 caagaatgca gacattgaag cccatctgga aagaaattac atcaccaagt aatcatcagg
     1621 ggggatatca gatgaatgaa aggtttattg aaacaagcca tattccttta acctatgtga
     1681 agaaggatgg cactaaagga agcttctatc tatccaatgg gaagaagggc atcttgccac
     1741 aaaggataag cttgggcatc tttgcccaga agaaggaaag ccagaaggac aagctgaaga
     1801 aggatgaaat caatgggaca tacaagaaga cagaggaagg gctttacaag tccctcaaca
     1861 atggcagggt acattctagt atatggggct ttgaagaata tccagaattt tatgggtatg
     1921 gcataattga tgaaaggtac gccctatatg acttggtaat aatgtactct gggaataatt
     1981 gttcatcttc ctttgatgtc catttattca ggggcatggg gaagcctgac ttcatccaga
     2041 aggcattcca ttatctcagg tgttatataa agcagaagcc ccaatgaagg ggcttcaggg
     2101 atattcaatg aacttggatt atattgacat atccaaattc ctagacaaag ataggatttg
     2161 ccttgactat ctgcaaagga atgccattta tgatggggct gaaatgtact atttcaatgg
     2221 atgcaggaag attgaagtct ggatgaggca ggcaaccaac caagtcaata tcaagggcag
     2281 tatcccctat ttcatcaatg ggcataattt ctatgccagc catcaggatt ggctagaagg
     2341 gctggactat attcagggat gtctgaatgt gaacatatat tcaggacagg tgaatagctt
     2401 tgaatttggc actattcagg aaatcccatt cccagaacct gacttcctca accatcatat
     2461 caaggtaagg ggcatggaag caagggcata tcacaagggg caagtcctga caggcaggga
     2521 atttgtgtcc ccttccctga agataaagct ttatgatgtc cagaggaaca taaagacaaa
     2581 gctggacaag cccatcaggg cagatttaag cagggattat ggctgggata gcaggaagca
     2641 ttacattaag ctggaaaacc attacaggca acctgaagcc tatttcaagg gcaatgtcca
     2701 gctatcagaa ctattatcat ccagcttcca gcaggacttg aagaaagggc tattaacatc
     2761 atatcagagc ataatgaaaa caggaacaac tgccatcccc aaaagcaagg ctgacatcaa
     2821 tgcagggaca attcccttga tgattttgaa ggaacttg
//
LOCUS       DAOPCH010000077         2803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_28335_length_2803_cov_1.984352, whole genome shotgun sequence.
ACCESSION   DAOPCH010000077 DAOPCH010000000
VERSION     DAOPCH010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28335_length_2803_cov_1.984352"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PLU55_04930"
     CDS             complement(<1..902)
                     /locus_tag="PLU55_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87436.1"
                     /translation="MTLDTVHLHLTDYSVQAENSLTIEPSPYKASTGELVSDFHLYTD
                     NEGRQVNGKKARWNTPHFQLTFAPFTKSLEGVSCFVQFSVPKVHYGNNYYSVGEQGSE
                     AVFKEIEKELWEGGVHTDISTCNITRLDTFKNIEPEENFIAYSPLFGLLKMRKGVDKK
                     YPESFLLRNTQQQFCVYDKIAEMKKRQVDTSPFPERSMRFEHRCMNKQKVQNVFGFSS
                     VESLFEGGYLTVKKRQVEEWKDSLFRYEVEEVMNLGSSVIEQEMRYFKEKYKRNWFDW
                     FLKSNGTRYLAEVAGVEVVRMALQ"
     gene            complement(1103..1564)
                     /locus_tag="PLU55_04935"
     CDS             complement(1103..1564)
                     /locus_tag="PLU55_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87437.1"
                     /translation="MTETAVAVNAIYSEQELKKDSQVSVRLESGLFEALETQTQVWGF
                     KSISQTVRAILTFYFLPVVYELELKNRSISEHKEFLKEKQEEGFSLEQAKANYFTFQV
                     VEYLEFLEQAMVMARHSLQFMESTSDKMNGILRETVNKIEQAMKELEQGAE"
     gene            complement(1666..1938)
                     /locus_tag="PLU55_04940"
     CDS             complement(1666..1938)
                     /locus_tag="PLU55_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87438.1"
                     /translation="MKGLVCRMETGEFPLLGNQFIAGGNKYVFRQRKKATVNKPVDYL
                     LQLQPQVEYVSSLFPTGEEGLYTFDYKRKVFVLKKNEYKVVIVEEG"
     gene            complement(2039..2449)
                     /locus_tag="PLU55_04945"
     CDS             complement(2039..2449)
                     /locus_tag="PLU55_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPJ87439.1"
                     /translation="MTKTLEQVGKFVELRAKGYSYDKIAEETGTSKPTLLKWSTEYSR
                     ELKEAEHFEMNSLLSQYGVLRQGRVQAFSSMLGSALQELKKRAEEKDYSEVPTDKLLR
                     IALELERRLEREAEEKKFDNGALGYFKEEEVQVD"
     gene            complement(2456..>2803)
                     /locus_tag="PLU55_04950"
     CDS             complement(2456..>2803)
                     /locus_tag="PLU55_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87440.1"
                     /translation="CEAGREYQQNTWNTVFGTEGEGTQLTVIQPLTAINGRVWETKYF
                     IPKQNNMNQKGTVLCLQEAGGVKCLQKWHWSVCGNVTYSKAKFRVGCLHGDRFGILQF
                     VAKFATGRNRKEG"
BASE COUNT          705 a          718 c          446 g          934 t
ORIGIN      
        1 ttttggagtg ccattcttac aacctcaacc cctgcaacct ctgccaaata ccttgttcca
       61 ttgctcttca gaaaccagtc aaaccagttc cttttgtact tctctttgaa gtacctcatt
      121 tcctgctcaa tgacacttga acccaagttc ataacctctt caacctcata cctgaacaga
      181 ctgtctttcc attcctcaac ctgcctcttc ttgactgtca agtacccacc ctcaaagaga
      241 ctttcaacag aagagaaacc aaacacattc tgaaccttct gtttgttcat gcacctgtgt
      301 tcaaacctca tacttctttc aggaaatgga gaggtgtcaa cctgcctctt cttcatttct
      361 gcaattttgt catacacaca aaactgctgt tgagtgttcc tcaacaggaa actttcaggg
      421 tattttttgt ctactccctt tctcattttg agtaaaccaa acaggggtga ataggcaatg
      481 aaattctctt caggttcaat gttcttgaaa gtgtcaagcc ttgttatatt gcaggtgctt
      541 atgtctgtat gaaccccacc ctcccacaat tccttttcaa tttctttgaa cactgcctct
      601 gaaccctgct ctcccactga atagtaattg ttgccataat gcactttagg aacagagaat
      661 tgcacaaaac aggagacacc ctcaagtgac tttgtgaagg gtgcaaatgt caactgaaag
      721 tgaggggtgt tccagcgtgc cttctttcca ttcacctgcc tgccttcatt gtcagtgtac
      781 aggtgaaagt cagaaactaa ctctccagtg cttgccttgt atggagaggg ttcaattgtc
      841 agactattct ctgcctgtac agagtagtca gtgaggtgca ggtgtacagt gtcaagtgtc
      901 ataatagttc ctatttgttg caaactttgc aatgaaacct gtattgttac tcaagcaggt
      961 ttcttttcag gaacagacag aatgactttt gttcaggggt attgcaaccc tcattatttg
     1021 ttgtataatg aagttaccca aacaaaacaa accaaaccct atgagaggaa accatttcca
     1081 gtggtttctt ttcatataca tttcattctg caccctgttc caactccttc attgcttgct
     1141 caattttgtt gactgtctct ctcaaaatac cattcatttt gtctgaagtg ctctccataa
     1201 actgcaggga gtgccttgcc ataaccattg cctgttcaag aaattccaaa tactcaacaa
     1261 cctgaaatgt gaagtagttt gcctttgcct gctccagtga gaaaccctct tcttgtttct
     1321 ccttcagaaa ttctttatgt tctgaaatgc tcctgttctt caactccagt tcatagacta
     1381 caggcagaaa gtaaaaagtc agtattgctc tcactgtttg ggaaatgctc ttgaaacccc
     1441 aaacttgagt ttgtgtttcc agtgcctcaa acaagccact ttccaaccta acagaaacct
     1501 gactgtcttt cttcagttcc tgttctgaat atattgcatt gactgcaact gctgtttctg
     1561 tcattgcctt gcctcctgac ttctctgcat gcaattcagt atactccttt gcatgcaata
     1621 atgcaagtta atacactgaa atacccctac cattttttta tttttttacc cttcttctac
     1681 aatgactact ttgtattcat tcttcttcag aacaaacact tttctcttgt agtcaaaggt
     1741 atacaaaccc tcttctcctg tagggaaaag agaagaaaca tactcaactt gaggctgtaa
     1801 ctgcagaagg tagtcaactg gtttgttgac tgttgccttc tttctctgcc tgaacacata
     1861 cttgttacca cctgcaataa actggtttcc caacaaaggg aattcacctg tctccattct
     1921 acaaaccaaa cctttcattt ttattaactt tctgctcttc ttgagtttcc agtctcaatt
     1981 acagaaggca gacaattccc tgttattgcc tgtataacag gaaagaaaca gtcaactttt
     2041 agtcaacttg tacctcttct tctttgaaat aaccaagtgc tccattgtca aacttcttct
     2101 cctctgcctc tctttccaac ctcctttcca actcaagtgc aattctcaaa agtttgtctg
     2161 ttggtacttc tgaatagtct ttctcctctg ctctcttctt cagttcctga agtgcactgc
     2221 ctaacattga ggagaaagcc tgaaccctac cctgcctcaa aactccatat tgtgaaagca
     2281 ggctgttcat ttcaaagtgt tctgcctcct tcaattctct tgagtattca gtgctccatt
     2341 tcagaagagt tggtttgctt gtgcctgtct cctctgctat tttgtcatat gaatacccct
     2401 ttgctctcaa ctcaacaaac tttccaacct gttccagtgt ctttgtcatt gttcctcaac
     2461 cctctttcct gttcctgcct gttgcaaact ttgcaacaaa ttgaagtatt ccaaacctgt
     2521 ctccatgcag acaccccacc ctgaattttg cctttgagta tgtcacattg ccacacacgc
     2581 tccagtgcca tttctgcagg catttcaccc cccctgcctc ctgaaggcac aaaacagtgc
     2641 ctttttggtt catattgttt tgtttgggaa tgaagtattt tgtctcccaa actctgccat
     2701 ttatggctgt taatggctgt ataacagtca attgagtacc ttctccttca gttccaaata
     2761 cagtgttcca agtgttttgt tggtactctc tgcctgcctc aca
//
LOCUS       DAOPCH010000078         2758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_28907_length_2758_cov_5.069922, whole genome shotgun sequence.
ACCESSION   DAOPCH010000078 DAOPCH010000000
VERSION     DAOPCH010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28907_length_2758_cov_5.069922"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1720
                     /gene="thrS"
                     /locus_tag="PLU55_04955"
     CDS             <1..1720
                     /gene="thrS"
                     /locus_tag="PLU55_04955"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPJ87441.1"
                     /translation="NLHVDYIKFKPLKKALKSIKELPDKDKKEKEIKEALVVLTAVEK
                     ADKSVPIVVKQLVENIKDIASQVKTKNIVLYPYAHLSKDLGSPEIAQEVLEKAEEELK
                     KHKFHVARAPFGYYKEFELKVKGHPLSELSREIRIDKEKEENKEDKIIKEEVYDSKQL
                     LREISKSKLDSSKLKDNDHRILGRKMDLFSFSEVAPGMVFWHNNGLITRNELINFWRE
                     EHKKAGYKEIQTPQILDKKLWQISGHWEKYKENIFLTEYEKRIFAVKPMNCPGGILVF
                     KNSPKSYKDLPLRVGELGVVHRQELSGVLGGLFRVIQFTQDDAHIYCTEKQIENELAK
                     VIDLVDLFYKKFGFEYTIELSTRPEKRIGDEKMWDLAENTLKKVLEKRKVKFKINEGD
                     GAFYGPKIDFHLKDSLERTWQCGTIQLDFAMPERFELEYIDSDNKKKRPIMIHRTIYG
                     SLERFIGMLLENTNGRLPTWLAPVQVRVLSFTDRNIDYAEKIITRMKELIPSIRIDFD
                     FEQKTVQSKVKDAEEMKIPYILVIGDKEEKDGVVSVRKGGSNLVGKMDTDEFIFKIRE
                     EIEKRI"
     gene            1879..>2758
                     /locus_tag="PLU55_04960"
     CDS             1879..>2758
                     /locus_tag="PLU55_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87442.1"
                     /translation="MSKKGVLIIFVVFLLALSFTSARNFTIYNSTNPSQAYFSIDGTT
                     GRVGIGTMNALQGLHVIGNILANGTINATRDICIQGGICLSGIAITASGEPLWTGNWT
                     NVAFTNTHETFNENVTFMKNISFSANANLQGIYFDSLYWVGERLAFGADSTGTIIQST
                     DNDVGDGIHFKAHGGGNDLMIINNTGYVGIGTSSPQERFHVAGSARIDGTLNTNSNKI
                     INIANGTNAQDAVTYSQLITQNTSQTNLINLNNQSVTNYINSVASIGEPNWNANYSIF
                     LTHATTNYVNAQNLSMR"
BASE COUNT         1101 a          341 c          479 g          837 t
ORIGIN      
        1 aaatctccat gtagattata taaaattcaa gccacttaaa aaagcactta aaagtattaa
       61 agaacttcca gataaagaca agaaagagaa ggaaattaag gaagctttgg ttgttttaac
      121 tgctgttgaa aaagcagata aatctgtccc tatagttgta aaacaattag ttgaaaatat
      181 taaagatatt gcttctcaag ttaaaaccaa aaatattgtt ctttaccctt atgctcacct
      241 ttccaaagat ttaggaagtc ctgaaattgc tcaagaagtt ttagaaaaag ctgaagaaga
      301 attaaaaaag cataaatttc atgttgcaag agctcctttt ggttattata aagaatttga
      361 attaaaagtt aaaggacatc ctttaagtga attgtcaaga gaaataagaa tagacaaaga
      421 gaaagaagaa aataaagaag ataagataat taaagaagaa gtttatgact ccaaacaatt
      481 gttaagagaa atttcaaagt ccaaattaga tagttctaag ctcaaagata atgaccatag
      541 aattttagga agaaaaatgg acttattcag tttttcagaa gttgctcctg gtatggtatt
      601 ttggcataat aatggattaa ttacaagaaa cgaattaatt aatttttgga gagaagaaca
      661 caagaaagca ggttataaag aaattcaaac tccccagatt ttagataaaa aactctggca
      721 aatttcagga cattgggaaa aatataagga aaatatcttc ctcacagaat atgaaaaaag
      781 aatttttgca gttaaaccaa tgaattgtcc aggaggaata cttgtattca aaaattctcc
      841 aaaatcttac aaagatttac ctttaagagt tggagaatta ggggttgttc acagacaaga
      901 attatctggt gttttaggag gattgtttag agttatacaa tttactcaag atgatgcaca
      961 tatttattgc actgaaaaac aaattgagaa tgaacttgca aaagttatag atttagttga
     1021 cttattttac aaaaaatttg gatttgaata cacaattgaa ctttcaacaa gaccagaaaa
     1081 gagaatagga gatgaaaaaa tgtgggattt agcagaaaat accttaaaaa aagttcttga
     1141 aaaaagaaaa gttaaattta agattaatga aggagatgga gcattttatg gtccaaaaat
     1201 tgattttcat ttgaaagact ctttggaaag aacttggcag tgtggaacaa tacaactcga
     1261 ttttgcaatg cctgaaagat ttgaattaga atatattgat agtgataata aaaagaaaag
     1321 gccaataatg attcacagaa caatttatgg aagtttagaa aggtttattg gaatgctttt
     1381 ggaaaataca aatggaagac ttccaacttg gttagctcct gttcaggtta gagttctaag
     1441 ttttacagat agaaatattg actatgcaga aaaaataatt acaagaatga aagaattaat
     1501 cccaagtata agaattgatt ttgattttga acaaaaaact gttcaatcaa aagttaaaga
     1561 tgctgaggaa atgaaaatac cttacatact tgtaataggc gataaagaag aaaaagatgg
     1621 agttgtttct gtaagaaagg gtggaagcaa tcttgttgga aaaatggata cagatgagtt
     1681 catatttaaa atacgagaag aaattgaaaa aagaatttaa ccatctttca ttctattttt
     1741 tgtttttctt aattatttca tgtaagtaaa aattttcact acaatttatc aaggagggaa
     1801 gaaaaacatt tataatatat tatatattac atagagcata ttataaatta taatttagta
     1861 tcaactttat tgattaaaat gagtaaaaaa ggggtgttga ttatatttgt tgtttttctt
     1921 ttagctttgt cttttacatc tgctagaaat tttacaatat acaatagcac caatccaagc
     1981 caagcatatt tttcaataga tggaacaaca ggcagagtag gtattggaac tatgaatgcc
     2041 cttcaaggcc ttcatgttat aggaaatatt ttagcaaacg gcacaattaa tgctaccaga
     2101 gatatttgta ttcaaggagg aatttgttta agtggaattg caataacagc aagtggagaa
     2161 cctttatgga caggtaattg gacaaatgtt gctttcacaa atactcatga aacatttaat
     2221 gaaaatgtaa ctttcatgaa aaacatttct tttagtgcta atgcaaatct tcaaggaatt
     2281 tattttgata gtttgtattg ggtgggagaa agattagctt ttggagcaga ctctacaggt
     2341 acaatcatac aatctactga taatgacgtg ggtgatggaa ttcacttcaa agcacatgga
     2401 ggaggaaatg atcttatgat aataaacaac actggctatg ttggaattgg gacaagttcc
     2461 cctcaagaaa gattccatgt tgctggtagt gctagaatag atggaacatt aaatacaaat
     2521 tcaaataaga taattaatat tgcaaatgga actaatgcgc aggatgctgt aacttactca
     2581 caattgataa ctcaaaacac ttcacaaact aacctaatca acttgaataa tcaatctgta
     2641 actaattaca taaattcagt tgcatcaata ggtgaaccta actggaatgc taattattca
     2701 atcttcttaa ctcatgcaac tacaaactac gttaatgctc aaaacctttc aatgagaa
//
LOCUS       DAOPCH010000079         2716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_29480_length_2716_cov_7.508831, whole genome shotgun sequence.
ACCESSION   DAOPCH010000079 DAOPCH010000000
VERSION     DAOPCH010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29480_length_2716_cov_7.508831"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..1382
                     /locus_tag="PLU55_04965"
     CDS             285..1382
                     /locus_tag="PLU55_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87443.1"
                     /translation="MYNKAVLLQTLVTTGSFGGKMIDTMKLMLTDYEITEDAEITVQP
                     SSFVLSTGAMVEHPLFNVKDKGTVYGSKAYANTENWNLTLKPVLGMGTGAFLQLSVPK
                     NHYGSNYYSVGEGGTKAVLNKVENELNDRGIKTNIQEAVLSRLDTFKNIEPEEHFSSY
                     YGLFQTLNAKRTAKRDYGTTFLYSNTQQEFCVYDKKEEMRNRKLNTEGLPETMRFEHR
                     LLNKTKINSVYGFTGVKDIFKGGYEVIREKQIESWRSNLFKYEADEVLVLGSRQIEEE
                     MRAYRERHGRNWLQYYIKSYGSYYLTQYAGKEAVGIALMNIEEDRVKAWRANKILEEA
                     ERELLNMKKIEGTNKTMAQLYEELKNKVCEN"
     gene            1409..1828
                     /locus_tag="PLU55_04970"
     CDS             1409..1828
                     /locus_tag="PLU55_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87444.1"
                     /translation="MAMTKEDKMKFIELRGKGWSFDKIAEEMKVSKPTLLKLNGDLFE
                     EVKEAQYFETEAIVEQYGLMRKERLKIHCNALKKAMGEFKKKVEEQDFEEMPIEKLYT
                     FIEQLEKRVEKDTDRAKVEIAVVPPDFSFYKDTVGID"
     gene            1868..2113
                     /locus_tag="PLU55_04975"
     CDS             1868..2113
                     /locus_tag="PLU55_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87445.1"
                     /translation="MITGKFKLQDKAFIAGGRRFLIRERKEAKNKPKQYLCEIKPFNY
                     ISSLFEAGGAGNYTFDFEGKVYTFKLNEGEVEVEELE"
     gene            2205..>2716
                     /locus_tag="PLU55_04980"
     CDS             2205..>2716
                     /locus_tag="PLU55_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87446.1"
                     /translation="MKEVEQAKNRKEPILTKEKGNKSRVLSLRVNAQIEQALQEQAEK
                     WELSLSDTVRSILNFYFLPVSYLKLWNEKAKELEQKDIEATGESRADEQAQTIAERIK
                     PLLVDADEAEEYATFLFELMNKNMQAIKVLTYEAGIMGAVTSEKLLELARTYESYYIL
                     PKQEQPVEEEV"
BASE COUNT          984 a          416 c          629 g          687 t
ORIGIN      
        1 cctatgagag ctattacata cttccaaaac aagagcaacc tgttgaggaa gaagttatgg
       61 ttgagatgct ggaggaaatg acagtatgaa aatgaagttc agttgcggaa catcttcaac
      121 ctcttcttcc tttgtggttc ttttgtcttt tgagtgtttg gagcttaaat gaaaaagaag
      181 tttcagagcg gaactcctca acctcttctt cctttgtggt gtgtcaatta tacattgctt
      241 tcacaaaatg gaacaagtga aaaggctcaa catcaaaaca gctcatgtat aacaaagctg
      301 ttttgttaca aactttggta acaactggct cttttggagg caaaatgatt gacacaatga
      361 agttaatgct gacagactat gagattacag aagatgcgga aataactgtt caaccttcat
      421 cttttgttct ttcaactggt gcaatggtgg aacatcctct tttcaatgtt aaggacaaag
      481 gaactgttta tggctcaaaa gcctatgcca atactgaaaa ctggaacttg actttgaagc
      541 ctgtactggg aatgggaaca ggagcttttc tccaactttc agttccaaaa aatcattatg
      601 gctcaaacta ttattcagtt ggagaaggtg gaacaaaggc tgtactgaac aaagtagaaa
      661 atgaacttaa tgacagaggc ataaagacaa acattcaaga agctgtattg agtaggctgg
      721 acactttcaa gaacattgaa ccagaagaac atttttcatc atattatggt ttgtttcaaa
      781 cactcaatgc caaaagaaca gccaaaagag attatggaac aacttttctt tattcaaaca
      841 ctcaacagga gttttgtgtt tatgacaaaa aagaagagat gcggaacagg aagctgaaca
      901 ctgaaggctt accagaaaca atgaggtttg aacacaggtt gctgaacaaa accaaaataa
      961 acagtgttta tggctttact ggagtgaaag acatcttcaa aggaggctat gaagttataa
     1021 gagaaaagca gattgagagc tggagaagca acctgttcaa gtatgaagca gatgaagttt
     1081 tggtgttagg ctctagacag attgaagaag aaatgagagc ttacagggag aggcatggaa
     1141 ggaactggtt acagtattac atcaaaagct atggttcata ttatttgaca caatacgctg
     1201 gcaaagaagc tgttggtatt gcattgatga acattgaaga agacagggtt aaggcatgga
     1261 gagctaacaa aattcttgaa gaagcggaaa gggagttgct caacatgaag aagattgaag
     1321 gaacaaacaa aacaatggca cagctttatg aggaactcaa aaataaagtt tgtgagaatt
     1381 aattattcag tgagaggagg aactgattat ggcaatgaca aaagaagaca aaatgaagtt
     1441 cattgagttg agaggaaaag gttggagttt tgacaaaatt gcagaagaaa tgaaagtgtc
     1501 aaagccaaca ttgcttaagc tcaatggtga tttgtttgaa gaagtgaaag aagcgcagta
     1561 ttttgagact gaagcaatag tggagcaata tggattaatg cgcaaggaga ggctcaaaat
     1621 tcattgcaat gcgctcaaaa aggcaatggg agagttcaag aagaaggttg aagaacagga
     1681 ttttgaggaa atgcctattg aaaagctgta caccttcatt gaacaactag agaagcgggt
     1741 tgagaaagac acagacagag ccaaagtaga gattgctgtt gttcctccag acttttcttt
     1801 ttacaaggac actgtgggaa ttgactaaaa ctttaccaaa atttaaccaa cattaggaga
     1861 aaggacaatg attactggaa aattcaaatt gcaagacaaa gcctttattg ctggagggag
     1921 aaggttctta atcagagagc gcaaggaagc caaaaacaag ccaaaacaat acctttgtga
     1981 gattaagcct ttcaattaca tctcttcatt gtttgaggct ggaggagctg gcaattacac
     2041 ctttgatttt gaaggcaagg tgtacacctt caagctcaat gagggagaag ttgaagttga
     2101 agagctggaa tagttcttct ttttttaact gaatactgaa aacataatgt ggtaaaattg
     2161 tatacatcaa gaacaaggag caagtgaaca aagaaggaga aggcatgaag gaggttgaac
     2221 aagcaaagaa caggaaagag ccaattctta ccaaagagaa aggcaataaa agcagagttc
     2281 tttctttgcg tgtcaatgca cagattgaac aggcattgca agagcaagct gaaaaatggg
     2341 aattaagttt gtctgacact gtgagatcta ttttgaactt ttacttcctc cctgtgtctt
     2401 acctcaaact ttggaatgag aaagccaaag aacttgaaca aaaagacatt gaggcaactg
     2461 gagaaagcag agcagatgaa caggctcaaa ctattgcaga gcggattaag cctttgctgg
     2521 ttgatgcaga tgaagctgaa gaatatgcaa ccttcctgtt tgagttaatg aataaaaaca
     2581 tgcaagcaat taaggtattg acatatgagg ctggaataat gggagctgta acaagtgaaa
     2641 agctgttgga gctggcaagg acctatgaga gctattacat acttccaaaa caagagcaac
     2701 ctgttgagga agaagt
//
LOCUS       DAOPCH010000080         2604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_31134_length_2604_cov_2.856807, whole genome shotgun sequence.
ACCESSION   DAOPCH010000080 DAOPCH010000000
VERSION     DAOPCH010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31134_length_2604_cov_2.856807"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="PLU55_04985"
     CDS             <1..1055
                     /locus_tag="PLU55_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87447.1"
                     /translation="NEYCDDGNNVNGDGCSKTCRFECPNGVCTPNITIEDFVPIVWQC
                     DSRVVYDDATRPGRITYDGEEMIERMNNYAFEGEQIKWKVLVMDKNGIDKVKDVYVTV
                     DGSIEANCRRMYGNQMDPTCNARILEEKLTSFNPSTMAYYECVLTIETSDSMYGPSEI
                     TVEAEDLNGERGQMDETEVWFLNPVVSLNVQGGVMFSQLRPGTMEYSIPVTLTNDADR
                     DSGVLLDVFVSGTNFYDSSSSGAKCPTTNQLSLKNIAYYAVNGAYSTVDDMEIGRACD
                     DEGYCGVNYGFEFHNPYKFYNRNEILQVQKVGPYYTGNLLSPGADMTLTFRVNVPEPC
                     IGNFNSGHIYFWAEAV"
     gene            1073..>2604
                     /locus_tag="PLU55_04990"
     CDS             1073..>2604
                     /locus_tag="PLU55_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87448.1"
                     /translation="MINKTTKTLKVLAFLIMLVGMFALTSASLIYSNNAQYDDTSHDY
                     DIPDSNVGYATVMVRGAGYYQYATLTFNYESGTKYTAPLQNAVYNQWLKMSFINPNTN
                     LKVTSITLTGSPAIYFKDVYVYGDISDKEEDGGDGGVVSCPTDKKRCADGSYVSRNVA
                     LNCAFDLCPEEKICGNGKIDLGEQCDEGENNGIKCSADYGSSCTYCSSNCEIITVLGE
                     YCGDGILNTMYERCDDGNRINNDRCTNECKKPFEVIVEDFEPLVWQCDSRVVYDDATE
                     PGRISMHGTPLVERINNYAFEGEQIKWRVLVMDKNGIDKVKDVYITVDGNIEANCRRM
                     NNYQYTPSNNDIDFSGISLFNFGGGSIGLKDKENETQTPEEGTQEENPTPDMIDPSCN
                     ARILEEKLTKFNPNTMAYYECVLTVETFESMQGDSEVLIEAIDLDDVVGTMDEVEYWW
                     FNPVIQLSIDGEVEFDEVRPGTSSYSRRVIIGNDAEYGSGVLLDMFISGTDFYDSSSS
                     GA"
BASE COUNT          948 a          315 c          552 g          789 t
ORIGIN      
        1 caaatgaata ttgtgatgac ggaaataatg ttaatggaga tggatgctca aaaacatgta
       61 gatttgaatg tccaaatgga gtgtgcactc caaatataac aattgaggac tttgttccaa
      121 tagtatggca gtgtgacagt agggtagtgt atgatgatgc tacaagacct ggaagaataa
      181 cttatgatgg tgaagagatg attgagagaa tgaacaatta tgcttttgaa ggagaacaaa
      241 tcaaatggaa agttcttgta atggacaaga atggtattga caaggttaaa gatgtttatg
      301 ttactgtaga tggaagtatt gaagctaatt gtagaagaat gtatggaaac caaatggacc
      361 ctacatgtaa tgcaagaata ttggaagaga aattaacaag tttcaatcct agcacaatgg
      421 cttattatga atgtgtttta actattgaaa cctctgattc aatgtatgga ccatctgaaa
      481 taacagttga agctgaagat ttgaatggag agaggggaca gatggatgaa acagaagtgt
      541 ggttccttaa tcctgttgtt tcattgaatg ttcaaggagg agtaatgttc agtcaattaa
      601 gaccaggaac aatggaatat tctatacctg taacattaac taatgatgca gacagagact
      661 caggagtttt attagatgta tttgtatctg gaacaaattt ctatgattca tcaagttcag
      721 gagctaaatg tccaactaca aatcaattaa gtttgaaaaa tattgcatac tatgctgtca
      781 acggagcata ttctactgtc gacgatatgg aaattggaag agcttgtgac gacgagggat
      841 attgtggtgt aaactatggt ttcgagttcc ataatcctta taaattctat aataggaatg
      901 aaatactaca ggtgcaaaaa gtgggtcctt attacactgg taacttactt tctcctggag
      961 cagacatgac acttacattc agagtaaatg ttcctgaacc ttgcatagga aacttcaact
     1021 caggacatat ttatttctgg gctgaagctg tttaaacaaa aaggaaaaca aaatgataaa
     1081 caaaacaaca aaaacattga aggtgcttgc attcttaatt atgcttgtag gtatgtttgc
     1141 attaacaagt gcatctctaa tttatagtaa taatgcacaa tatgatgata cttcacatga
     1201 ttatgatatc ccagattcta atgtaggata tgcaacagta atggttagag gagcaggata
     1261 ttatcaatat gcaactttaa cttttaatta tgagagtgga acaaaatata ctgctccatt
     1321 acaaaatgca gtttataacc aatggttgaa aatgagtttc attaatccaa acacaaatct
     1381 aaaagttaca agtataacat taactggtag ccctgcaata tacttcaaag atgtttatgt
     1441 ttatggtgat atttcagaca aagaggaaga tggtggagat ggaggagttg tttcatgtcc
     1501 aactgacaag aaaagatgtg cagatggttc atatgtttca agaaatgttg cattgaattg
     1561 tgcatttgat ttatgtcctg aagaaaaaat ctgtggaaat ggaaagattg atttaggaga
     1621 acaatgtgat gaaggtgaaa acaatggaat aaaatgttct gctgattatg gttcttcatg
     1681 cacttattgt tcaagtaatt gtgaaataat aactgttctt ggggaatact gtggagatgg
     1741 aatattaaat acaatgtatg aaagatgtga tgatgggaac agaataaaca atgatagatg
     1801 tacaaatgaa tgtaagaaac catttgaagt tattgttgaa gattttgaac cattagtatg
     1861 gcagtgtgac agtagagtag tttatgatga tgcaactgaa ccaggaagaa tatctatgca
     1921 tggaactcct ttagttgaaa gaataaacaa ttatgctttt gaaggagaac aaatcaaatg
     1981 gagagttctt gtaatggaca agaatggtat tgacaaggtt aaagatgttt atattactgt
     2041 agacggaaat attgaagcta attgcagaag aatgaataat tatcaataca caccaagcaa
     2101 caatgatatt gattttagtg gaatttcttt gtttaatttt ggtggaggtt caattggact
     2161 taaagataaa gaaaatgaaa cacaaactcc agaagaagga actcaagaag aaaatccaac
     2221 acctgatatg attgaccctt catgtaatgc aagaatatta gaagagaaac taacaaaatt
     2281 caatcctaat acaatggctt attatgagtg tgttttaact gttgaaacat ttgaatcaat
     2341 gcaaggtgac tctgaagtat tgattgaagc tattgattta gatgatgttg ttggaacaat
     2401 ggatgaagtt gaatattggt ggtttaatcc tgttatacag ctttcaattg atggagaagt
     2461 tgaatttgat gaagtaagac ctggaacaag ttcatattca agaagagtta taatagggaa
     2521 tgatgctgaa tatggttcag gagttttact tgatatgttt atatctggaa cagactttta
     2581 tgattcatca agctcaggag ctaa
//
LOCUS       DAOPCH010000081         2508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_32721_length_2508_cov_17.730942, whole genome shotgun
            sequence.
ACCESSION   DAOPCH010000081 DAOPCH010000000
VERSION     DAOPCH010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32721_length_2508_cov_17.730942"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1921)
                     /locus_tag="PLU55_04995"
     CDS             complement(<1..1921)
                     /locus_tag="PLU55_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87449.1"
                     /translation="MAEPMQVKLVIFDTTHTRLLGKTDIIASFDATRAVKTIDIDPIK
                     FIDEDTLMVSERKCTITFGNIYNSYKYVKDRLDTIEELIATVHKNFNSYYWDNYGIDY
                     DDINNTRIINYIKPVIKLKDDTLYSDLALHILAYPVYYLTSNSIGYIPIINEDEDKYN
                     RYDEVYKKERGKYHNILVEVYFYDKLQYVGVALVNTLKFNIKSQEWSLEFTDAIGVMI
                     TCLDKLGSLPSLYQDVDYSGLFLKHLYDIPKITIEMMYPTLRDIFNYKGFYTYYGSSI
                     TLTVKCTYGVNHSSLGCLYDFKLEDSNGAIAINTLPSNNGYRLAPRVDNTDGGGFPFY
                     QYEKYIHYRVRKDNQNNNIAEIDVLFTEIYRERNDSSGQWLNSYHILRTSLQIVFGLS
                     SCIYYCGNRNTDTIWGYDDYVDVLDVVLFEYMNKIGGTWINSGISANWDTGVYPGAIA
                     SYNYGFTANNPITFGTSPTPSQEMQNLFTAYNDEGFNSQISILDAYKLHLLSKVETIY
                     SRGYDIIRKPFKKSTTTNFTVNGRYIKELTIEPYYYEKPDLSVIENITNWEGKTKDEL
                     HTRVNLKDWTASLMETIPPGQTEKITVAIILKDTYYNDIEIGNKIRIYPLYYCLPDEP
                     DTDDLYFIVTSIKWNI"
     gene            complement(1923..>2508)
                     /locus_tag="PLU55_05000"
     CDS             complement(1923..>2508)
                     /locus_tag="PLU55_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87450.1"
                     /translation="VKKIDDRAQQEHRSALWSKKQKEKLDKEREKKERQLMKFKRAVS
                     IAEIAYNTAVAVMRIFKDFSIPISYVLAGIVSSLGLAQIALVNKQKFAKGGKVYGASH
                     YNGGVPVELEGGEFIMSKKATTGNEAVLYAIQAMLSSKQVPNMSDNIVVDKLTKLTDA
                     VNNLELKAEFKGQVLNEVQLYKKTVKGQRLARVM"
BASE COUNT          827 a          451 c          306 g          924 t
ORIGIN      
        1 ttatattcca ttttatagat gttactataa aatataaatc atcagtgtca ggttcatctg
       61 gcaaacaata atataaagga taaatcctta tcttattgcc tatctctata tcattataat
      121 aagtgtcttt gaggataatg gcaacagtta ttttttctgt ttgacctgga ggtatagttt
      181 ccattaagga agcagtccaa tccttaaggt ttactctggt atgcaattca tctttcgttt
      241 taccttccca attggtaatg ttttcaataa ccgataaatc tggcttttca taataataag
      301 gttctatagt caattcttta atgtatcttc cattaacagt aaaattggta gttgtgcttt
      361 tcttaaatgg ttttctgata atatcataac ctctgctata aatagtttcc acttttgaaa
      421 gcaaatgtag cttataagca tccaagatac tgatttgact attaaaccct tcatcattat
      481 aagcagtgaa cagattttgc atttcttgag aaggagtagg agaagttcca aaagttatag
      541 gattatttgc agtaaatcca taattatagc ttgcaatagc acctggataa actcctgtat
      601 cccaattagc actaatacca ctatttatcc aagttccacc aattttattc atatactcaa
      661 acaatacaac atctaaaaca tctacataat catcatatcc ccatatagta tccgtatttc
      721 tattgccaca ataatatata caagaactca aaccaaaaac tatttgcaga gatgttcgca
      781 aaatatgata actattaagc cattgtccag aactatcatt tctttcacga taaatctcgg
      841 tgaataaaac atctatttct gctatattat tattttgatt atccttgcga actctataat
      901 gaatatattt ttcatattga taaaacggga aacctccacc atcagtattg tcaactcttg
      961 gtgctaatct gtatccatta ttggatggta aagtatttat agcaatagca ccattgctat
     1021 cttctaactt aaaatcatat agacaaccta aactactatg gttaacacca taagtgcact
     1081 taacagttaa tgttatagaa cttccataat aagtataaaa acctttataa ttaaatatat
     1141 ctcttaatgt aggatacatc atttctatgg taattttagg aatatcataa agatgtttca
     1201 aaaatagacc agaataatct acatcttgat aaagagatgg caaacttccc aatttatcaa
     1261 gacaagtaat cataacccca atagcatctg taaattctaa tgaccattct tgacttttaa
     1321 tattaaactt aagagtattg actaacgcta ctcctacata ctgaagctta tcataaaaat
     1381 aaacttctac aagtatatta tggtatttac ccctttcttt tttataaact tcatcatatc
     1441 tgttatattt atcttcatct tcattgataa ttggaatata gcctatacta ttagatgtta
     1501 aataatatac gggataagct aatatgtgta aagctaaatc agaatataga gtatcatcct
     1561 ttagtttaat gacaggcttt atgtagttga taattcttgt gttgttaatg tcgtcataat
     1621 ctatgccata attatcccaa taatagctat taaagttttt atgaacagtt gctataagtt
     1681 cttctattgt atctaatcta tcttttacat atttgtagct attataaata ttaccgaaag
     1741 ttatagtgca ctttctttct gataccatta aagtatcttc atcaatgaac tttatagggt
     1801 ctatatctat tgtttttaca gctcttgtag catcaaaaga agcaattata tcagttttgc
     1861 ctaataaacg agtatgggta gtatcaaata ttaccagttt tacttgcata ggttctgcca
     1921 ttttacatta cccttgctaa cctttgacct ttaacggtct ttttgtataa ctgcacttca
     1981 tttaatacct gtcctttgaa ttctgccttt aattctaagt tgttaacagc atctgttaac
     2041 ttagtcaatt tgtcaactac aatattatct gacatattag gaacttgctt actggataac
     2101 attgcttgaa tagcatataa tactgcttca ttaccagtag tagccttctt ggacattata
     2161 aactctccac cttccaactc tacaggaaca ccaccattat aatgagaagc accatagacc
     2221 ttaccacctt tagcaaactt ctgtttatta actaaagcta tttgtgctaa acctaaagaa
     2281 gatacaattc cagctaacac ataactaata ggtatactga aatccttaaa tattctcata
     2341 acagcaactg ctgtattata agcaatttct gcaatagaaa ctgctctttt gaacttcatt
     2401 agttgtcttt ctttcttttc cctttcctta tctaatttct ctttttgttt tttagaccat
     2461 aaagcagacc tatgctcttg ttgagctcta tcatctattt ttttgact
//
LOCUS       DAOPCH010000082         2455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_33664_length_2455_cov_4.207917, whole genome shotgun sequence.
ACCESSION   DAOPCH010000082 DAOPCH010000000
VERSION     DAOPCH010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33664_length_2455_cov_4.207917"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2455
                     /locus_tag="PLU55_05005"
     CDS             <1..>2455
                     /locus_tag="PLU55_05005"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPJ87451.1"
                     /translation="FNTTVRNCNIYNYTAGIFFNGADNGTIENNNIIIYNNNSLDSII
                     HDNSVTARASGILIYDSANYNTIVNNTAYSYYGRGIYINDASNNIIKNSNGSSYFNPG
                     IQVFGGLGHGGSHNSIINSFGLSEESNGIRLGYYGYYDSIINSTAISNLDYAILADYG
                     EFIDVINSFANSSSNTGIHFLGVQNGNIINSTGFSNLTNGIEITYSSHNNIISNSTGI
                     GYHTGIVLGSVPSYPSNNNLLIDSQGTGEIAGIRLGGINNTIIRNRATGENEAYHLSS
                     LNNSRIIDCVYTSGNHQDIYVSSVYLSVNNTFLNCSYDDEYFTGSSGEIIRKWYYRAY
                     TNYSNGTAVAGANITAYNTTGNIQFTELTDSSGWIPRQEITEYVNTGGTRRYYNNYTI
                     DATLSDYETDSNIFNFTTTQNKVNDVFTLNELVPGENACGTLNSANTVYTLTSNVSSQ
                     GTCFTITQPNITLDCAGYWINYSINGLTDTYGIYTNQFNTTIKNCNIVDGNYSSINNN
                     RQGIYFYQSSNGTIENNKVISNNSQAVHLRLNSEFNTVKNNTIHGFSNTGLDMHTSNN
                     NNITNNLMITNSSANSGLLVYQSSNNVFLNNTVMGEGYGIWIMGTSSISSNDNLFING
                     TAISNVGDAIKLDAGKDNTFINQIAISYSYGDYAISINSANHTRFVDCVNLSSSPHQI
                     YVHYRTALNNSFINCSYDSEYVREDGTENELIRKWYYQAYTNYSNGTAVAGANITAYN
                     SSGQLQFTTLTNPSGLINRQEVTEYVNTGGTRSYYNNYTIDATLSGYEIDSNVFNFTT
                     TKNKLDDVFTLNELDKFISV"
BASE COUNT          942 a          342 c          366 g          805 t
ORIGIN      
        1 aattcaatac tacagttaga aattgtaata tatataatta tacagcaggt atcttcttta
       61 atggagcaga taatggtaca atagaaaata ataatataat catttacaat aacaactctc
      121 tcgattctat tattcatgat aatagtgtaa cagcaagagc ttctggtatc ttaatttatg
      181 attcagctaa ttacaataca attgtaaata atacagcata ttcttattat ggaagaggta
      241 tttacataaa tgatgcaagc aacaatataa tcaaaaactc taatggaagt tcttatttta
      301 atccaggtat tcaagtattt gggggtttag gacatggtgg ctcacataac tcaataatta
      361 attcttttgg tttatcagaa gaaagcaatg gtattaggtt aggatattat gggtattatg
      421 acagcattat caattcaaca gctatttcaa atttagacta tgcaatttta gctgattatg
      481 gagaatttat agatgtaatt aattcttttg caaactcttc gtctaacaca ggaattcact
      541 ttttaggagt tcaaaatgga aatatcatta attcaacagg attctctaat ctaaccaatg
      601 gtatagaaat tacttatagt tcacataaca atataatttc taattcaaca ggaatcggat
      661 atcatacagg tattgtctta ggaagtgtac cttcttaccc atcaaacaat aatcttttaa
      721 ttgattctca aggtactggg gaaatagcag gtattcgctt aggaggcatc aataatacta
      781 ttataagaaa tagggctaca ggagaaaatg aagcatatca tctttctagt ttgaataatt
      841 ccagaattat agattgtgtt tatactagtg gaaatcatca ggacatatat gtatcttctg
      901 tgtatttaag tgtaaacaac actttcctta attgttctta tgatgatgaa tattttacag
      961 gaagttcagg agaaataata agaaaatggt attatcgggc atacaccaat tattctaatg
     1021 gaactgcagt tgcaggggca aatataactg cttacaatac aactggtaat attcaattta
     1081 cagaacttac agattcatct ggttggatac caagacaaga aattacagaa tatgttaata
     1141 caggtggaac aagaagatat tataataatt atacaataga cgcaacactt tcagattatg
     1201 aaacagatag taatattttc aactttacaa caacccaaaa taaggttaat gacgtattta
     1261 cattaaatga attagttcca ggagaaaatg catgtggaac attaaattct gcaaacacag
     1321 tttatacatt aacttcaaat gtttcatctc aaggaacttg tttcacaata actcaaccaa
     1381 acataactct tgattgtgca ggatattgga taaattattc tataaatggt ttgacagata
     1441 cttatggaat ctatacaaat caattcaata caacaataaa gaattgtaat attgttgatg
     1501 gaaattattc aagtataaat aacaataggc aaggaatata cttttatcaa tcaagtaatg
     1561 gaacaattga gaacaacaaa gttatttcaa ataactcaca agctgttcat ttgagactca
     1621 attcagaatt taatactgtg aaaaataata ctatacacgg attttctaat actggtttag
     1681 atatgcatac ctcaaataat aacaatatca caaacaattt aatgataact aattctagtg
     1741 caaattctgg attacttgtt taccaatctt caaataatgt atttttaaat aacacagtaa
     1801 tgggagaagg atatggaatc tggattatgg gaacttcctc aatatcttca aatgataatc
     1861 tcttcattaa tggtactgct ataagtaatg ttggagatgc tattaaatta gatgctggaa
     1921 aagataatac ttttataaat caaatagcaa tttcttattc ttatggagac tatgcaatat
     1981 caataaatag tgctaatcac acgaggtttg ttgattgtgt gaatttatct tcatcacctc
     2041 atcaaatata tgtccattac agaactgctc tcaataactc atttataaat tgttcttatg
     2101 attcagaata tgttagagaa gacggaacag aaaatgaact aataagaaag tggtattatc
     2161 aggcatacac caattattct aatggaactg cagttgctgg agcaaacatt acagcatata
     2221 attcatctgg acaattacaa ttcactacat taaccaatcc aagtggatta attaatagac
     2281 aagaagttac agaatatgtt aatacaggtg gaacaagaag ttactataat aattacacaa
     2341 ttgatgcaac actttcaggt tatgaaatag atagtaatgt tttcaacttc actacaacta
     2401 aaaataaact tgatgatgtg tttacattaa atgaattaga taaattcatt tcagt
//
LOCUS       DAOPCH010000083         2365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_35417_length_2365_cov_25.432035, whole genome shotgun
            sequence.
ACCESSION   DAOPCH010000083 DAOPCH010000000
VERSION     DAOPCH010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35417_length_2365_cov_25.432035"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PLU55_05010"
     CDS             <1..428
                     /locus_tag="PLU55_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87452.1"
                     /translation="VGIMKLWADFGFPAAVPLTAFLSALSVAQIAMVASQKYGKGGLV
                     TGASHYNGGVPVELEGGEFIMSKKATTGNEAVLYAIQAMLSSKQVPNMSDNIVVDKLT
                     KLTDAVNNLELKAEFKGQVLNEVQLYKKTVKGQRLARVM"
     gene            430..>2365
                     /locus_tag="PLU55_05015"
     CDS             430..>2365
                     /locus_tag="PLU55_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87453.1"
                     /translation="MAEPLKVILKTFAEPGGMGFGSPTITMTADVTDAVKNIDIDPTC
                     FIDEDTLLVSERKCSITLINRYNIYKTIKDMLTDIEEDITTTEYNYNNLQELDGESGS
                     RYVKFIYPYIRCHHSKYTTSLIATSEFYYPVIYKEPTDGRRISYIPILEFDENNNLED
                     RYINYDNAYKKERPKYHNLLIEVYFYDILQYVGIALINTLKFNIKTNEISIDFTDPIG
                     VMIDCLNKINSFPNFYQGIVNTGLAMDFLYDLPRRTIEMFYHRLKNLFNFRKFLGRIT
                     ANYRLFATCKYGSNFDHTFYDFNVYDGNTEIFNSEDPIGGHGANRDPRNSNRHPNEGF
                     SNYWQYKKYIYYKVCKDGSENEPEIHIIFFEIYRKRSGSNWTNDYAYIITRIRIILGN
                     TNAPYFMKDSHGKGNGFFATWDIFFNAVITNTLATAGGTWIKATMDENWDTGSYPTNI
                     TSTLYYAPIPDTGYYIHTNTLTYNQNLYNAYNDEGFNEQLSLLDAFKLHLLSRVDTVY
                     SKGYDIIRKPFKNSTAGSGYYDFGINGRYVKELTIEPTYYEKPDLSAIEMIANWEGKK
                     RAPGLTTLVNLKDWTYELMETFPSGNAQKVKVTFIIGDNTYSDLELGNKVKISPLYNC
                     FPDEPDTDDLYFIVTSIKWNI"
BASE COUNT          845 a          315 c          429 g          776 t
ORIGIN      
        1 ctgtaggtat aatgaaactt tgggcagatt ttggttttcc tgcagctgtt cctcttactg
       61 cttttttgag tgctttaagt gtagctcaaa ttgcaatggt agcatctcag aagtatggta
      121 aagggggatt ggtaacaggt gcttctcatt ataatggtgg tgttcctgta gagttggaag
      181 gtggagagtt tataatgtcc aagaaggcta ctactggtaa tgaagcagta ttatatgcta
      241 ttcaagcaat gttatccagt aagcaagttc ctaatatgtc agataatatt gtagttgaca
      301 aattgactaa gttaacagat gctgttaaca acttagaatt aaaggcagaa ttcaaaggac
      361 aggtattaaa tgaagtgcag ttatacaaaa agaccgttaa aggtcaaagg ttagcaaggg
      421 taatgtaaaa tggcagaacc tttgaaagta atacttaaga cctttgcaga acctggaggt
      481 atgggttttg gttctccaac tattacaatg actgcagatg tgacagatgc agttaagaat
      541 atagacatag atcctacttg ttttatagat gaagatactt tattggtatc ggaaaggaag
      601 tgttccataa ccctaatcaa cagatataat atatacaaaa ctatcaaaga tatgttaaca
      661 gatattgagg aagatataac tactacagaa tataattata ataatttaca agaacttgat
      721 ggagaatctg gttcaagata tgttaagttt atatatccat atataagatg tcatcacagt
      781 aaatatacta cttctcttat agcaacaagt gagttctatt atcctgtaat atataaagaa
      841 ccaactgacg gaagacgtat atcttatatt ccaatcttag aatttgatga aaacaacaat
      901 cttgaagata gatatataaa ttatgataat gcatataaaa aggaaagacc aaaatatcat
      961 aacttattga tagaagttta tttctatgat atcttgcaat atgtaggtat agccttaatc
     1021 aatacattga agtttaatat taagaccaat gagatctcta tagattttac agatccgata
     1081 ggagttatga tagattgtct caacaagata aatagttttc ctaactttta tcaaggtatt
     1141 gttaatactg gtttagcaat ggactttctt tatgatttac ccagaagaac catagagatg
     1201 ttctatcata gattgaaaaa tctatttaat tttagaaagt ttttaggcag aataactgca
     1261 aattataggc tatttgctac ctgtaaatat ggttcaaatt ttgaccatac attctacgat
     1321 tttaatgtat atgatggaaa tacagaaata tttaactctg aagatccaat aggaggtcac
     1381 ggagctaata gagatccaag aaatagtaac agacatccca atgagggttt tagcaattat
     1441 tggcagtata aaaaatatat ctattataag gtttgtaaag atggaagtga aaatgaacct
     1501 gagattcata ttatcttttt tgaaatctat cgtaaaagaa gcggatctaa ttggactaat
     1561 gattatgctt atataataac tcgtattagg ataatcttag gcaacacaaa tgctccttat
     1621 tttatgaaag atagtcatgg aaaaggtaac ggatttttcg ctacttggga tatttttttt
     1681 aatgctgtaa taaccaatac attagctact gctggtggaa cctggataaa ggctacaatg
     1741 gatgaaaatt gggatacagg aagttatcct acaaatataa cttctactct ttattatgca
     1801 cccataccag atacaggtta ttatatccat accaatacct taacttataa ccaaaacctg
     1861 tataatgctt ataatgatga aggtttcaat gaacagttat ctttattgga tgcttttaag
     1921 ttacatctat tatcaagagt agatactgtc tatagtaaag gttatgatat tatcagaaaa
     1981 ccttttaaga atagcacagc tgggtcaggc tattatgatt ttggcataaa tggcagatat
     2041 gttaaagagt taacaataga acctacttat tatgaaaagc ctgatttatc tgctatagag
     2101 atgattgcta actgggaagg caaaaagaga gctccaggtc ttacaacttt ggttaacctt
     2161 aaagactgga cttatgagtt aatggagaca tttccttctg gtaatgctca aaaggttaaa
     2221 gttactttta ttattggtga taatacttac agcgatttgg agttgggtaa taaggttaaa
     2281 attagtcctt tatataattg ttttcctgac gaacctgaca ctgatgattt atattttata
     2341 gtaacatcta taaaatggaa tataa
//
LOCUS       DAOPCH010000084         2301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_36741_length_2301_cov_2.112199, whole genome shotgun sequence.
ACCESSION   DAOPCH010000084 DAOPCH010000000
VERSION     DAOPCH010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36741_length_2301_cov_2.112199"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="PLU55_05020"
     CDS             complement(<1..1035)
                     /locus_tag="PLU55_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87454.1"
                     /translation="MFDKIALHTKDFTIKNKSPFHIKMGEMDCSTGEAIYSFPLFTYG
                     GKIIEGSKAYIPDTNKQLPYQLDINTNGLLISYNPSKIIFPNTCYLADNEKLKLCNDM
                     LFTELKEYIDTDFEKLELIRIDLAKNIKTDLCCFEYNELFRTLSASRMKEIEYPEGYY
                     FGNRSRQIVFYDKVRELKEVQKISNEYFKDLPANILRSEARFLKKSVIEKNIGFKEIG
                     KLYSLENYKNIEQVYKNFISSVVFKKQVNDQLELFAYADELEKLKHYKTLYQRNSIDR
                     YIGDYGLKEILNIYNGDLNLFKKALMEVGYERTYVYSYIKSLEERILQSSFIEKRYNK
                     SNKTLKGMYDE"
     gene            complement(1218..1631)
                     /locus_tag="PLU55_05025"
     CDS             complement(1218..1631)
                     /locus_tag="PLU55_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87455.1"
                     /translation="MKTKLINVRIEPAVLELLENKAKENSISISAYIRNMASIEIAAD
                     LISQRMVNRLKDLKRMEKKNDVEIVEFADKHLSFFKYLISSISNQIKTLTVLEKNLLK
                     DYENISDVLKNNFEKAVEAMEKERCINEGKNKKEN"
     gene            complement(1884..2165)
                     /locus_tag="PLU55_05030"
     CDS             complement(1884..2165)
                     /locus_tag="PLU55_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87456.1"
                     /translation="METTKIKYEGKYKKAGSKIFNTEIELLIKERKKLLNKTKYFLME
                     KIGNKYIYLSSLFEQDKENTYLFDDRKNNYIMRIIGTEIIIELEKVEKG"
BASE COUNT          745 a          357 c          177 g         1022 t
ORIGIN      
        1 ttcatcatac attcctttta atgttttgtt tgatttgtta tatctctttt ctataaaact
       61 actttgtaat attctctctt ctaaactttt aatgtaactg taaacataag ttctctcata
      121 tccgacttcc attaatgctt ttttaaataa gtttaaatca ccattataaa tatttaagat
      181 ttcttttaaa ccataatcac ctatatatct gtctatgcta tttctttgat acaatgtttt
      241 ataatgtttt aatttttcca attcatcagc ataggcaaat aattctaact ggtcatttac
      301 ttgtttttta aataccactg atgaaataaa gtttttataa acttgctcaa tatttttata
      361 attttctaaa gaatataatt ttcctatttc tttaaaacct atattctttt caattactga
      421 tttctttaaa aatcttgctt cacttcttaa aatatttgca ggaaggtctt taaaatactc
      481 attactaatt ttctggactt cttttaattc tctcacttta tcataaaaaa caatttgccg
      541 gcttctattt ccaaaataat acccctctgg atattctatc tctttcattc tacttgcact
      601 taaagttcta aaaagctcat tatattcaaa acaacataaa tcagttttaa tgtttttagc
      661 taaatcaatt ctgatcaatt ctaatttttc aaaatcagta tcaatgtatt cttttaattc
      721 agtaaaaagc atatcattac ataatttcaa tttttcatta tcagctaaat aacaagtatt
      781 aggaaaaata atctttgaag ggttataaga aattaataaa ccatttgtat taatatctaa
      841 ttgataaggt agttgtttat ttgtatctgg tatatatgcc ttactgcctt caataatttt
      901 tccaccataa gtaaataatg gaaaagaata tattgcttca ccggtagagc aatccatttc
      961 acccattttt atatgaaaag gacttttatt ttttatagta aaatctttag tatgtaatgc
     1021 tattttatca aacatacata ccccatttaa tagaatgttg tctataagac aacaaatacc
     1081 ctattgatat acataaggta tttgttaaaa agaataatct gattttttgt ttgatttcaa
     1141 tatagacttg aattataatt tcattaacaa acaaacaaaa caaaaaaaga agaggactac
     1201 agctaccttt ttttagctta gttctctttt ttatttttac cttcatttat acaccgttcc
     1261 ttttccatag cttctacagc tttttcaaaa ttatttttta atacatcact tatattttca
     1321 taatctttta aaagattttt ttctaaaact gttaaggttt ttatttgatt actaatactt
     1381 gaaattaaat atttaaaaaa acttaaatgc ttatcagcaa actctacaat ttcaacatca
     1441 tttttctttt ccattctttt taagtctttt aatctattta ccattctttg tgaaattaaa
     1501 tccgctgcta tttctatact tgccatattt ctaatatatg cactgataga aatagagttt
     1561 tcttttgctt tgttttccaa taactctaaa actgccggtt ctattcttac attaattaat
     1621 tttgttttca attcttatcc ttatatttaa ttataataca aatataatta attgtatgac
     1681 attacatttt taatttgtct tacaataaat actaatactt atttttttat atttcaatta
     1741 aattattatc tttactaagg tttaccccta tttattaagt tttccttaga tttattgaca
     1801 ttttacaatt tcattacagt atatattaag tgaagcaaat tgcacttttt aaagtttccc
     1861 atatatcttt tcacttactt ttcttaacct ttttctacct tttccaattc aataattatt
     1921 tcagttccaa taattctcat tatataattg ttttttctat catcaaataa ataagtgttt
     1981 tccttatctt gctcaaataa gctgcttaaa taaatatatt tgttacctat tttttccatt
     2041 aaaaaatact ttgttttatt taataatttt tttctctctt taataagtaa ttctatttca
     2101 gtattaaata tcttactgcc tgctttttta tatttacctt catactttat tttagttgtt
     2161 tccattaaaa taaaccttcc ttgcatacct gattaaactt tgcaaagtca aaccaccttt
     2221 ttttatatgc agattttact atataattca tcatacattc cttttaatgt tttgtttgat
     2281 ttgttatatc tcttttctat a
//
LOCUS       DAOPCH010000085         2233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_38228_length_2233_cov_3.078053, whole genome shotgun sequence.
ACCESSION   DAOPCH010000085 DAOPCH010000000
VERSION     DAOPCH010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38228_length_2233_cov_3.078053"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>878
                     /locus_tag="PLU55_05035"
     CDS             <1..>878
                     /locus_tag="PLU55_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87457.1"
                     /translation="GTVSDGCGGTLTCGNYNGGCQTGYTCQTDRGFCVQNPCVPATCA
                     SLGRNCGTVSDGCGGTLTCGNYNGGCQTGYTCQTDRGLCVASTPSYYCGDGACNNDET
                     CSSCPEDCGQCQPTNYLRTFYVSSSSGNDANSGTSPSSPWKTLSKVNSQTFRAGDAIL
                     FKRGDSWYGSLTVSNSGSSSAPITYGAYGTGNKPVISGFKTISGWTSSGGGTYYQGVS
                     TESSPLIVTVNGKNTPKGRWPNTGWILFDSYSGYTSITDKELNGKNFVNGEVVIRVAK
                     WITYRAKITSHSGSTI"
     assembly_gap    879..888
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <889..>2233
                     /locus_tag="PLU55_05040"
     CDS             <889..>2233
                     /locus_tag="PLU55_05040"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPJ87458.1"
                     /translation="TINFPWELGGDYSLKSGQGFFIQNDVDTFDIVGDWAYVNGNLYM
                     YFGSNSPSNYNVRVSTLDSFAKLNRREYITFDNLYLEGYNTHGIEVRNSRHITMTNCE
                     FQNMGGSGIYGPWDGDSSYMKVENCKFIENNNCGIKVMGNHENAIIRYNTFKNIGMID
                     GLAGIRDGSAIGITSGSKKTLISNNNIEYAGYNGITFRGEDSKVINNFINHFCQKKDD
                     CGGIYTYEDSGTGKQILDNVVMNSLNHNEGIDESSRDFHAHGIYIDGSDNVQIHRNTV
                     ANNEGAGIFLNTQALGIDLQNNLAFGNVRGIQIISNWRGMGEITGLIMRNNIFVAKES
                     THHTLYYTTGSGNEGVKKLGSVNYNYYARPIDDTKTFRNQIYLWNGPTTYRTLAEWQS
                     YSGLDSNSKKSPKSISSTSDLRFEYNPTSSSKTINLDRNYIDIKGERYSGSLVLKP"
BASE COUNT          796 a          242 c          480 g          705 t
ORIGIN      
        1 gtggaacagt aagtgatgga tgtggaggga ctttaacttg tggaaattat aatgggggtt
       61 gtcaaactgg atatacttgt caaacagata gagggttttg tgtacaaaat ccatgtgtac
      121 ctgcaacatg tgcttcatta ggaagaaatt gtgggacagt aagtgatgga tgtggaggga
      181 ctttaacttg tggaaattat aatgggggtt gtcaaactgg atatacttgt cagacagata
      241 gaggattgtg tgttgcatct actccttctt attattgtgg agatggggct tgtaataatg
      301 atgaaacttg tagttcatgt ccagaagatt gtgggcaatg tcaaccaaca aattatttga
      361 gaacatttta tgtttcaagt tcaagtggaa atgatgcgaa tagtggaact tcaccttctt
      421 ctccttggaa aaccttaagt aaagttaatt ctcaaacttt tagagctgga gatgcaatat
      481 tatttaagag aggggatagc tggtatggtt ctttaactgt gagcaattca ggcagttctt
      541 ctgcaccaat aacttatggt gcatatggta ctggaaataa acctgtaata agtggattta
      601 aaactataag tggatggact tcttctggtg gaggaacata ttatcaagga gtttcaacag
      661 aatcaagccc attaattgta actgttaatg gaaagaatac tccaaaagga agatggccaa
      721 atacaggatg gattttattt gattcttatt caggttatac ttcaataaca gataaggagt
      781 taaatggtaa aaattttgta aatggggaag ttgtgataag agttgcaaaa tggataacct
      841 acagagcaaa gattacaagt cattctggtt ctacaatann nnnnnnnnac aataaatttc
      901 ccatgggaat taggaggaga ttattcactt aaatctggac aaggattttt catacaaaat
      961 gatgtagata catttgatat agttggggac tgggcatatg ttaatggaaa tctgtatatg
     1021 tattttggtt caaatagtcc ttcaaattac aatgtaagag tttcaacatt agattctttt
     1081 gcaaaattga atagaagaga gtatataaca tttgataatt tatatttaga aggatataat
     1141 actcacggaa tcgaggttag aaattcaaga catataacta tgactaactg tgaatttcag
     1201 aatatgggtg gaagtggtat atatgggcct tgggatggag attcttcata tatgaaagtt
     1261 gagaattgta aatttattga gaataataat tgtggtataa aggttatggg aaatcatgaa
     1321 aatgctataa ttagatataa tactttcaag aatattggaa tgatagatgg tttagctgga
     1381 attagagatg gttctgctat tggaataacg agtggttcta aaaaaacatt aatttcaaat
     1441 aataatatag aatatgctgg atataatgga ataacattta gaggtgagga ttctaaagtt
     1501 attaataatt tcattaatca tttctgtcag aaaaaagatg attgtggagg aatttatact
     1561 tatgaagata gtggaacagg gaaacagatt ctagataatg ttgtaatgaa tagtcttaat
     1621 cataatgagg gaattgatga atcttcaagg gactttcatg cacatggaat atatatagat
     1681 ggttcagata atgttcagat acatagaaat acagttgcaa ataatgaggg agcagggata
     1741 tttttgaata cacaagcatt aggaattgat ttgcagaata atctggcatt tggtaatgta
     1801 agaggtattc aaataatttc aaactggaga ggaatgggag aaataacagg attaattatg
     1861 agaaataata tttttgttgc aaaagaatct acacaccaca ctctttatta tactacaggt
     1921 agtgggaatg aaggagtaaa gaagttgggt tctgttaatt ataattatta tgctaggcca
     1981 atagatgata caaagacttt caggaatcag atatatcttt ggaatgggcc aacaacttac
     2041 agaacacttg cagaatggca atcatattct ggattagata gtaattctaa aaaatctcca
     2101 aagagtattt ccagtactag tgatttaaga tttgaatata atcctacaag ttcaagtaaa
     2161 accataaatt tagacaggaa ttatattgat attaagggag agagatattc tggaagttta
     2221 gttttgaaac ctt
//
LOCUS       DAOPCH010000086         1924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_46902_length_1924_cov_36.141787, whole genome shotgun
            sequence.
ACCESSION   DAOPCH010000086 DAOPCH010000000
VERSION     DAOPCH010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46902_length_1924_cov_36.141787"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..552)
                     /locus_tag="PLU55_05045"
     CDS             complement(10..552)
                     /locus_tag="PLU55_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87459.1"
                     /translation="MLKKIGIILSIISILLICIYLIKWHKQHYINVKNITSTVDTVYV
                     EAVKVVPQIKYVKIPITHTVNDTIIIKDSLSYEVAVLDTTFQLEDKGWHKLHLEYDEY
                     YNLFDINSQYNLNSTQIITENPQKEQLKKLIKLGYGYDWQSNQPVLDLGIGLRYKALG
                     LGIEADTNNKLGLFLQWEFK"
     gene            complement(763..1407)
                     /locus_tag="PLU55_05050"
     CDS             complement(763..1407)
                     /locus_tag="PLU55_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87460.1"
                     /translation="MVETQNPAVIIGTLAFVTAIANALVSYFVNYKPNKRIEKISREL
                     NEFDYNNQEAHKSIMSKLEEHTNAIDPYIKQQSINQRLTDIAKHAIDWTDKTEITLLI
                     DGLIGLLIDFVQDIIEIGLDNLSEEQLKIKLSSLKIDLTKQVREYLNKLDVEKVKELE
                     CLYNNYALAVMDIREDDINDKTSRLFIKTEDFAHTVLKKSLKYFVEVLDEQSVK"
     gene            complement(1455..>1924)
                     /locus_tag="PLU55_05055"
     CDS             complement(1455..>1924)
                     /locus_tag="PLU55_05055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPJ87461.1"
                     /translation="EEIFFFVKQEHYYFQTQYEPFATPNLGDLKVSVGDKYKDENVKV
                     ENKSIQKFYNKVKDGEVEGRIMRSVWKVAVSNNGCFHKAPYPEQLIVPILNAVCPPNG
                     IVLDPFMGTGTTAIVALKQDKHFIGIDLDNRAIEYAQNRINSYLEQTTIFSHI"
BASE COUNT          597 a          330 c          260 g          737 t
ORIGIN      
        1 ttttatctgt catttgaact cccattgcag aaataaacct aatttattat tagtatctgc
       61 ttctattcct aatcctaaag ccttatatct gagacctata cctaagtcta agacaggttg
      121 attagattgc caatcataac cataacctaa cttaattaac ttctttaact gctctttttg
      181 tggattttct gtaataattt gagttgagtt aaggttgtat tgagagttaa tgtcaaataa
      241 gttataatat tcatcatatt ccaaatgtaa cttatgccag cctttatctt ctaactgaaa
      301 agtagtatct aagactgcta cctcataact tagactgtct ttgattataa tggtatcatt
      361 tacagtatga gttatcggta tcttaacata tttgatttga gggactactt taacagcctc
      421 tacataaact gtatccacag ttgaagttat attctttaca ttgatataat gttgcttatg
      481 ccatttgatt aaatatatac atatcaataa gatagatatg atacttaaaa taattcctat
      541 tttttttagc ataacttact gtttatcaga tctttaatct gttctatcag ttcctttaat
      601 tctttgattg tcagttcttg tgctttattt tctatgatat tccataaacc atagataata
      661 cttactgtta accatacaat aatagcttga gatcctgtta ttttaccaaa ccagcaaaat
      721 aaagaggcta ctataaaagt taacagaaaa agcaagaact ttctacttaa cagattgttc
      781 atctaacacc tccacaaaat atttgagact tttcttgaga acggtatgag caaagtcttc
      841 tgttttaata aataacctgg atgttttatc gttaatatca tcttctctaa tatccataac
      901 agctaaagca taattgttat aaagacattc cagttccttg accttttcca catctagttt
      961 attcagatat tctctaactt gcttagttaa gtctatttta agcgaactta acttgatttt
     1021 taattgttct tcagaaagat tatccaaacc tatctctatt atatcttgca caaaatcaat
     1081 caataatcct atcaatccat ctataagtaa ggtgatttct gttttatctg tccagtctat
     1141 agcgtgttta gctatatctg ttaatctttg gttaatgctt tgttgtttaa tataaggatc
     1201 tatagcattg gtatgttctt ctaacttact cataatactt ttatgagctt cttgattatt
     1261 gtaatcaaac tcattcagtt ctctacttat cttttcaatt cttttgttag gtttatagtt
     1321 gacaaaatag gacactaaag cattggctat tgcagtgaca aaagctaaag ttcctatgat
     1381 aacagcagga ttttgtgttt ctaccatttt taactcccaa agacagagaa aagaccataa
     1441 tctaacttgt caagttaaat atgggaaaat atggtagttt gttccaagta actgttaatc
     1501 ctgttttgag catattctat ggctctgttg tctaagtcta ttccgataaa atgtttatct
     1561 tgttttaagg ctactatggc agtagttcct gttcccataa atggatctaa tacaattcca
     1621 ttgggaggac atacagcatt taagatagga actataagtt gttctgggta aggtgcttta
     1681 tggaaacaac cattattgga aactgctacc ttccagacag atctcattat tctaccttca
     1741 acttctccat ctttgacttt attatagaac ttctgaatag atttgttctc tactttaaca
     1801 ttctcatctt tgtatttatc tccaacactt actttcagat ctcccaaatt gggagttgca
     1861 aagggttcat actgagtttg aaaataatag tgctcttgtt taacaaagaa gaagatctct
     1921 tcat
//
LOCUS       DAOPCH010000087         1745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_54093_length_1745_cov_2.875148, whole genome shotgun sequence.
ACCESSION   DAOPCH010000087 DAOPCH010000000
VERSION     DAOPCH010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54093_length_1745_cov_2.875148"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLU55_05060"
     CDS             complement(<1..290)
                     /locus_tag="PLU55_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87462.1"
                     /translation="MIEKQIKSFSKDTQKKAEAIGFNYLEQKYSGQTITEETIENEYQ
                     EGIESGAIDYYSENIEKQETPEEVETEEEYSEEQIQEAMKQIDEDTILQLCE"
     gene            complement(305..619)
                     /locus_tag="PLU55_05065"
     CDS             complement(305..619)
                     /locus_tag="PLU55_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87463.1"
                     /translation="MDCQKGQESNSDCERDCGKGDSKNMNRPTRKQMKEIKKMGFNSV
                     EELQEALKKEIANQRKQLRNQENDKFLSDNEEEDFNFDFLEDFELDLPDLDFDFDFLD
                     EE"
     gene            complement(695..991)
                     /locus_tag="PLU55_05070"
     CDS             complement(695..991)
                     /locus_tag="PLU55_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87464.1"
                     /translation="MTEEAIRQRRAYQRRYRALNRKKISEYNKKYFEENKERLKEVRQ
                     KWREENRDKVNEYHREWRKENKDKVKKYNQNFWNKKAIEFSEWEKSKQNDERNS"
     gene            complement(1010..1219)
                     /locus_tag="PLU55_05075"
     CDS             complement(1010..1219)
                     /locus_tag="PLU55_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87465.1"
                     /translation="MTRTKKIEDIKGNQISQNEEGILVNEETGNSLAWDILQLMGKTT
                     RRMIVANIIEGIVILILVAILFFKI"
     gene            complement(1239..1433)
                     /locus_tag="PLU55_05080"
     CDS             complement(1239..1433)
                     /locus_tag="PLU55_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87466.1"
                     /translation="MKTRKNDSKYSISLYNDKLKQLIIAISKKKNTTTTKYIEEALQI
                     QVEKDKNLLEKEELFRIVMK"
     gene            complement(1430..>1745)
                     /locus_tag="PLU55_05085"
     CDS             complement(1430..>1745)
                     /locus_tag="PLU55_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87467.1"
                     /translation="YQMNKWESISLDEKESIINFDYFEGIVNVYTTNQSTGNRIKKKI
                     GEPFKTGLNEGKIFSMEWKIPFNDREKIRKGLSIVNFITTHQSNRVEERLTNKEGGIE
                     TK"
BASE COUNT          378 a          336 c          234 g          797 t
ORIGIN      
        1 ttctcacaaa gttgaaggat tgtgtcttca tcaatttgtt tcattgcttc ttggatttgt
       61 tcttcagaat attcttcttc tgtctcgact tcttcaggtg tttcttgctt ttctatattc
      121 tcggaatagt agtcaattgc tcctgattca atgccttctt gatattcatt ttcaatcgtt
      181 tcttctgtga ttgtttgtcc tgaatacttt tgttcaagat agttgaatcc gattgcttcc
      241 gctttctttt gtgtatcttt cgaaaaagat ttgatttgtt tttctatcat ttggaatcct
      301 cccttcattc ttcatctaaa aagtcaaaat caaagtcaag gtcaggaagg tcaagttcaa
      361 agtcctctaa aaagtcaaag ttgaagtctt cttcttcatt atcagacaaa aacttgtcat
      421 tctcctgatt cctcaattgt ttcctttgat ttgctatttc ttttttgagt gcttcttgta
      481 gttcctcaac tgaattgaat cccattttct tgatttcttt catctgtttt cttgtaggtc
      541 tattcatatt tttggaatct ccttttccgc aatctctttc acaatctgaa ttgctttctt
      601 gtcccttttg acaatccatt gttcaataaa atcttcataa gtaggaaaga ctttgcaaag
      661 acttgttttg tagtagtgtt ctttcagtat cttttcaaga gtttctttca tcattttgtt
      721 tcgacttctc ccactctgaa aactcgattg cctttttgtt ccaaaagttt tgattgtatt
      781 ttttgacttt gtctttgttc tcttttctcc attctctgtg atattcattg actttgtccc
      841 tgttttcttc tctccacttt tggcggactt ctttcaatct ttccttgttt tcttcaaaat
      901 atttcttgtt atattctgaa atcttttttc tattcaatgc cctgtatctt ctttgatatg
      961 cccttctttg tctgattgct tcttcagtca ttgtctttcc ctccttcttt tatattttaa
     1021 agaataaaat tgcaactaaa atcaaaatca caattccttc aataatattt gcaacaatca
     1081 ttcttcttgt agtttttccc ataagttgaa gaatgtccca cgcaagagaa tttcctgttt
     1141 cttcattcac aaggattcct tcttcatttt gagaaatttg attgcctttt atgtcttcaa
     1201 tctttttagt tcttgtcata ttggttgcct ccttcctttt atttcatcac aattctgaaa
     1261 agttcttctt tttcaagtag gttcttgtcc ttttcaactt gaatctgaag tgcttcttca
     1321 atgtattttg ttgtagtagt gtttttcttt tttgatattg caatgattag ttgtttcaac
     1381 ttgtcattgt atagtgaaat agaatacttt gaatcatttt ttcttgtttt catttggttt
     1441 caatgcctcc ttctttattt gtcaatctct cctccactct gtttgattgg tgcgttgtga
     1501 taaagttcac aatcgaaagt ccttttctga ttttctccct gtcattgaat gggattttcc
     1561 attccattga aaagattttt ccttcgttca atcctgtttt gaatggttct cctattttct
     1621 ttttgattct gtttcctgtt gattggtttg ttgtatatac attcacaatc ccttcaaagt
     1681 agtcaaaatt gatgattgac tctttttcat caagtgaaat cgattcccat ttgttcattt
     1741 gatag
//
LOCUS       DAOPCH010000088         1360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_78438_length_1360_cov_4.770115, whole genome shotgun sequence.
ACCESSION   DAOPCH010000088 DAOPCH010000000
VERSION     DAOPCH010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78438_length_1360_cov_4.770115"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1360
                     /locus_tag="PLU55_05090"
     CDS             <1..>1360
                     /locus_tag="PLU55_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87468.1"
                     /translation="RQEVIEYVNTGGTKNYYNNYTINASKSGYEDDTNVFNFTTTKNK
                     VNDVFTLGGEFVDTVSPNYLLINHTSVAEFGDIPNYWIEQAKKLTLHYAHTSHGGQLI
                     QGANYIESNIDPKYNIAVREATTEGLPDDLTALRVYDGNPPETYIGPEDYWDNVSDPD
                     ALSRTMGVANTGKYNISMWSWCGQQADNSVSQVERYLNNMYWLQQQYPNMTFILMTGH
                     LGSQNNEYRYSNSTRDILKRNNDMVRNFAIEHNMTLFDFADIEGHNGTNYCYTNDYHV
                     AYNNTIVECKWPSSPSFYCSHSAGPNCERKGSAFWVMMAKIAGWDGQSNTAPVTSLNS
                     PSSGSSLSPGQITFQCNLADDYDLKNTTLYVWNSAGNLISSDLTVDVTGTSNSTNIIY
                     TINNAGSYSWNCLTYDGDSASSFSSTNNTFTIAGLISEENTCRELNSANTVYTLTQNV
                     SS"
BASE COUNT          497 a          202 c          228 g          433 t
ORIGIN      
        1 atagacaaga agttatagaa tatgttaata ctggaggaac aaaaaattat tacaacaatt
       61 acacaataaa tgcaagcaag tcagggtatg aagatgatac taatgttttc aacttcacta
      121 caactaaaaa taaggttaat gatgtgttta cattgggagg agaatttgta gatactgttt
      181 ctcctaatta tcttttaata aatcatactt ctgttgcaga atttggagat attcctaatt
      241 attggattga acaagcaaag aaattaacat tacattatgc acacacttca cacggaggac
      301 aacttataca aggagctaat tatatagaat caaatataga ccctaaatac aatattgcag
      361 taagagaggc cactacagaa ggtttacctg atgaccttac tgcattaaga gtatatgatg
      421 gaaatcctcc tgaaacatat attggaccag aagattactg ggataatgtt tcagatccag
      481 atgctctatc aagaactatg ggagtagcaa atacaggaaa atacaatatt tctatgtgga
      541 gttggtgtgg acaacaggca gataattcag tatcacaagt agaacgttat cttaacaaca
      601 tgtattggtt gcaacaacaa tatccaaata tgacttttat tttaatgact ggacatcttg
      661 gaagtcaaaa caatgaatac agatattcaa attctactag agatattttg aaaagaaata
      721 atgatatggt gagaaatttt gctattgaac ataatatgac tctttttgac tttgcagata
      781 tagaaggaca taatggaact aattattgtt atacaaatga ctaccatgta gcatataata
      841 acacaattgt agaatgtaaa tggccttctt caccttcttt ttattgttct cattcagcag
      901 gtccaaactg tgaaagaaaa ggaagtgctt tctgggttat gatggcaaag attgcaggat
      961 gggatggtca gagtaatact gctccagtta catctcttaa ttctccttca agtggttcta
     1021 gtttatctcc tggacaaatt acctttcaat gtaatttagc agatgattat gatttaaaga
     1081 atacaactct ttatgtttgg aattctgcag gaaatttaat ctcttctgac ttaactgttg
     1141 atgttactgg aacctctaat tcaactaata taatttatac aataaataat gcaggcagtt
     1201 attcatggaa ttgtttgact tatgatggag attctgcatc tagcttctct tcaacaaata
     1261 atacatttac aatagcaggt ttaatatctg aagaaaatac atgtagagaa ttaaattcag
     1321 caaacacagt ttatacatta acacaaaatg tttcatctca
//
LOCUS       DAOPCH010000089         1327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_81516_length_1327_cov_2.715409, whole genome shotgun sequence.
ACCESSION   DAOPCH010000089 DAOPCH010000000
VERSION     DAOPCH010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81516_length_1327_cov_2.715409"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            680..910
                     /locus_tag="PLU55_05095"
     CDS             680..910
                     /locus_tag="PLU55_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87469.1"
                     /translation="MIFNRIKYLYQKKNRYLQAYLQDLISTETGFPPYEIKLNKVAKI
                     DDSEAFNFFDFNYQGLHYELKNKVLRIVNDYE"
     gene            1080..>1327
                     /locus_tag="PLU55_05100"
     CDS             1080..>1327
                     /locus_tag="PLU55_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87470.1"
                     /translation="MNINFENIPRQMKEEGKFCLHENKIPKKRRYSKLYNASPNNKND
                     FSSYDSIMKDYNSIKSDKIGVGIGIFDSLCGIDLDHCF"
BASE COUNT          557 a          209 c          207 g          354 t
ORIGIN      
        1 aaagaaaaaa tcatcaaatc tttttcaatt caagatgata ttacaatcac actttttcaa
       61 tatcaagaaa aataattcta ctaaacttca cacctaaaag gaggcgtgaa aagttgtaga
      121 agtaggcgtg aaaagttgta tcttgaggcg tgaacagttg tcgattcagg cgtgaaaagt
      181 tgtagacaaa acaccctttt tccccttata gaataaggga ttgcgtttcc gatataatat
      241 atataataaa tataacttat atgagaaaag gaagacttat tgaaaaagtc ttcttttcct
      301 tttacaatga gagtgtgaat ctgacaagga atatctgaaa gcattttcac accgagacac
      361 cgccgagaac tccgagacat aaaaaaagag ggacaaccta gacaccgagt tttttcccac
      421 tataaaaaaa aagaaagttt ttgttttcgg cactattaaa aaaggagatt gattcatctt
      481 gcttgtgaac ttgaaatccg acttttccga ctccctaaaa aaagagaaac atttttccga
      541 ttttacagac caaaaaaaag gaagaaaatc aatgacaact tatttctata aattatcatt
      601 gaagcaaaaa aatgataaaa aatgatattt cctgaaatag aaaaaatgaa acaataattt
      661 tcaagaggag gcacaagaaa tgattttcaa tagaatcaaa tatctatatc agaaaaaaaa
      721 cagatacctt caggcatact tgcaagactt aatttcaacc gagacaggat tcccacctta
      781 tgaaatcaaa ttgaacaaag ttgcaaagat tgatgattca gaagcattca atttctttga
      841 ttttaattat cagggactac actatgaatt gaaaaacaaa gtcttgagga ttgtcaatga
      901 ctatgaataa aacaaaaaag caacctcaat gaatgaagtt gcaatctata aaaaccgata
      961 aatgaacgga aatcggacaa ccaagtgtca agaaggaggc acaccaattc ccaagacaag
     1021 aattgattcc ttcaagacaa actaattata tcataaaaaa agaaagagag gcacacttta
     1081 tgaatataaa ttttgaaaat ataccaagac aaatgaaaga agaaggcaaa ttctgtcttc
     1141 acgaaaacaa aatccctaaa aaaaggagat attcgaaact atacaatgca agtccaaaca
     1201 ataagaatga cttttcttct tatgattcaa ttatgaagga ctataattca ataaaatcag
     1261 ataaaatagg cgtaggaata ggaatctttg attcactttg tggaattgac cttgaccatt
     1321 gtttcaa
//
LOCUS       DAOPCH010000090         1300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_84146_length_1300_cov_2.518876, whole genome shotgun sequence.
ACCESSION   DAOPCH010000090 DAOPCH010000000
VERSION     DAOPCH010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84146_length_1300_cov_2.518876"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PLU55_05105"
     CDS             <1..592
                     /locus_tag="PLU55_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87471.1"
                     /translation="LRLLALPYASESKGYFSGYIISNTQRQVCFYDKYLQLLEVKKIE
                     ERALKGIVRAEYRLLNSESVKGAGLDFKQVLGYDSLKDAYRGFFDKVFRGVSSTSNDL
                     ILSSLAEKYQACKANKKPDVFIMALLMESGGFIDIEQVLERLDREGAFDKSNNHHSRE
                     KKRLRDAFNLAIRLASKEKITVLERYEQLKKALLKT"
     gene            820..1167
                     /locus_tag="PLU55_05110"
     CDS             820..1167
                     /locus_tag="PLU55_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ87472.1"
                     /translation="MPAIKEKFKENSKNNPVKKTMQPDSLSNIEKALLSLKMIEIEQE
                     KYIVKQRKYQTGKPNLYLWNLTGSKYGSSVFQTESSNILWVDFRAHPEQEPRKFYLIY
                     DLQGLGAWTVQKA"
BASE COUNT          459 a          175 c          285 g          381 t
ORIGIN      
        1 tttaaggttg cttgccctgc cttatgcctc agagtcaaaa ggatactttt caggctatat
       61 catttcaaat actcagaggc aagtttgttt ttatgataag tatttacagc ttttagaagt
      121 aaaaaaaatt gaagaaagag ctttgaaagg gattgttaga gcagaataca ggttgcttaa
      181 ttcagaatca gtcaaggggg caggtttgga ttttaagcag gttttaggtt atgattccct
      241 aaaagatgct tacaggggct tttttgataa ggtttttagg ggtgtgtcat caacttctaa
      301 tgatttaata ttatcttcac ttgctgaaaa atatcaggca tgcaaagcta ataaaaaacc
      361 tgatgttttt attatggctt tactgatgga gtcagggggg tttattgaca tagaacaggt
      421 tttagaaaga cttgacaggg agggggcttt tgataagagc aataaccatc atagcaggga
      481 aaagaaaagg ctgagagatg cctttaatct tgcaattaga cttgcaagta aagaaaaaat
      541 aacagttctt gaaagatatg aacaactaaa aaaagcttta ctaaagactt gaaagctttg
      601 cttgcattgg ttatattggt tatatttata ggtagggtga gaaaaggggg ttgattatga
      661 taagaatatt agaacaatag caaagaacag gattaaaagt ttttaagggg ttcttttaat
      721 cctgttttgc ctttgccttt tatgtgtgcc ttaaaaaggt aatcatagaa caggcaaaag
      781 gcaaaaaacc cctaccaact aaattaaaag gggggcaaaa tgcctgcaat caaagaaaaa
      841 ttcaaagaaa attcaaaaaa caatccagta aaaaaaacca tgcagccaga cagcttaagc
      901 aatatagaaa aagccttatt aagcttgaaa atgattgaga tagaacaaga aaaatacatt
      961 gttaagcaaa ggaaatacca gacagggaaa cccaatcttt atttgtggaa tctgacaggc
     1021 tcaaaatatg gttcaagtgt gtttcagact gaaagttcaa atattctttg ggttgatttt
     1081 agggcgcacc cagaacaaga gccaaggaag ttttatctga tttatgattt acagggttta
     1141 ggggcttgga ctgttcagaa ggcttaatcc ggtttaaggg ctaacttccc ttatgtatat
     1201 caatagggaa gttagccaga atttggctaa ggggtgtgta aatgattgac actataagcc
     1261 ttacagtaaa agactttgag gttgaaaatg gcaggctttt
//
LOCUS       DAOPCH010000091         1262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_87995_length_1262_cov_2.859155, whole genome shotgun sequence.
ACCESSION   DAOPCH010000091 DAOPCH010000000
VERSION     DAOPCH010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87995_length_1262_cov_2.859155"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..>1262
                     /locus_tag="PLU55_05115"
     CDS             358..>1262
                     /locus_tag="PLU55_05115"
                     /inference="COORDINATES: protein motif:HMM:NF036423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPJ87473.1"
                     /translation="MKPAKFKAFLFPKPYSDGKFPIYIRIYQNRKSSYVSIGHSIPAG
                     AWNEDNDEAWEAMPSLTPKLKATLTKEEQKAFREMQKSIILLPNAQKINSDIRAKIGE
                     LESLQNRLSANGAEVSSGILKDKADRKDIAELARMDFLSYIDEVIRKKFAGKQIRTSE
                     KYSVLLKKIKAFRKDKPLPIEELTTSFLKGFQAFLKGEGCHQNYIHVNLKALRTIIQK
                     DAIKEDKILPPEKNPFIWFTMPKVLPTIKEKLDIDEIKRIEDLKLKEDDSLFHVRNAF
                     IFSLYNAGIRIGDLMQLRWSNIKEDG"
BASE COUNT          416 a          259 c          265 g          322 t
ORIGIN      
        1 gattcttccc aaatccatgc agaaacatct gccatggcta tccatctgag ggcttcaaaa
       61 atgccccaaa tcacatattt gatttagtta tttgttagtc aggaaaagaa aaagccccgt
      121 attcatctag attacaggac tttaacctct tattcttgct ccccagccag agggggaatg
      181 ccatccccca gcctctttcc ttcttcctgc aaagttaagc ataaatacag atattcaata
      241 tcttgaaaag aatagtgcag aaataggaag aaaaagaatg taagattttg ttttagttat
      301 ttgttagctg gctaaatcat ttgtatctta gcaatggata tttaatgaac atgaattatg
      361 aagccagcca aattcaaggc tttccttttc cctaagcctt attcagatgg gaaattcccc
      421 atttacatca ggatttacca gaacagaaaa tcaagctatg tttccattgg gcattccatt
      481 cctgctgggg catggaatga agacaatgat gaagcctggg aagctatgcc cagcctaact
      541 cccaagctga aggcaacctt gacaaaggaa gaacagaaag cattcaggga aatgcagaag
      601 tcaatcatcc ttcttcccaa tgcccagaag attaattcag acatcagggc aaagataggg
      661 gaactagaaa gccttcagaa caggctttca gccaatgggg cagaagtatc ttcaggcatc
      721 cttaaagaca aggcagacag aaaggatatt gctgaacttg ccagaatgga cttcctcagt
      781 tatattgatg aggtaatcag gaagaaattt gctgggaagc aaatccgcac atctgagaag
      841 tattctgtcc tattaaagaa gataaaggct ttcaggaagg acaagcccct gccaatagaa
      901 gaacttacaa cttccttcct gaagggcttc caagcttttc tgaaagggga aggatgccat
      961 cagaactaca tccatgtaaa cctgaaggct ttgaggacta tcattcagaa ggatgccatt
     1021 aaggaagaca agatactgcc ccctgagaag aatcccttca tctggtttac tatgcctaaa
     1081 gtccttccca caatcaagga aaagctggac attgatgaaa tcaaaaggat agaggacttg
     1141 aagctgaagg aagatgattc cctgttccat gtcaggaatg cctttatttt cagcctgtat
     1201 aatgcaggca tcagaattgg ggacttgatg cagttaagat ggagcaatat caaggaagat
     1261 gg
//
LOCUS       DAOPCH010000092         1105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_3464
            NODE_108411_length_1105_cov_2.183810, whole genome shotgun
            sequence.
ACCESSION   DAOPCH010000092 DAOPCH010000000
VERSION     DAOPCH010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865199
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 18:49:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,024
            CDSs (total)                      :: 999
            Genes (coding)                    :: 997
            CDSs (with protein)               :: 997
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108411_length_1105_cov_2.183810"
                     /isolate="AMR_MDS_3464"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PLU55_05120"
     CDS             complement(<1..1072)
                     /locus_tag="PLU55_05120"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HPJ87474.1"
                     /translation="MKIDEIREISREELQEIIETYMKRPENTFQPTNQTSITYDEGMK
                     SDEQFFKIGLEKDDKLIQLWNETPSGSGGNESETDQALMNKIAFWTNKNETLMKLYFE
                     QSPYFQRKDDYHQKKWNTREDYQQSTISKAIASTNETARSKNIDFVNNMKERELQNNQ
                     PEVLIAKQPEEPKIDLDSLSTGKRIDRFFEIIEKQTFKSIPTGFKTFDEKTNGGFLNQ
                     SLITLGGGTSTGKTTFAINLALNFAKSRPVLYYSLEMGEEMIQAKIFSNLSYTGGGMT
                     IPSDNFLKCYDKNIMTEYQQSKVREELAKHEELNQLFIRYPQECNIDYILKEVKLFND
                     ELEKQGKEKAILFVDYLQYLQGN"
BASE COUNT          273 a          184 c          176 g          472 t
ORIGIN      
        1 gatttccttg taaatattga agataatcaa caaacaagat tgccttttct tttccttgtt
       61 tttcaagttc atcattgaaa agtttgactt ctttcaaaat atagtcaatg ttgcattctt
      121 gagggtatct gatgaaaagt tgattcaatt cttcgtgttt cgctagttct tccctgactt
      181 ttgattgttg gtattcagtc ataatgtttt tatcataaca tttcaagaag ttatctgaag
      241 gaatagtcat tcctcctcct gtataggaaa gatttgagaa gatttttgct tgaatcattt
      301 cttctcccat ttccaaagaa taatacaaga caggtctcga ctttgcaaag ttaagtgcaa
      361 gattgattgc aaaggttgtc tttcctgttg aagttcctcc tcctaaagtt atcaatgatt
      421 gattcaagaa tcctccgttt gttttttcat caaaagtctt gaatcctgtc ggaatactct
      481 tgaaggtttg tttttctatt atttcaaaga atcggtcaat cctttttcct gtcgaaagtg
      541 aatcaaggtc aatcttcggt tcttcaggtt gttttgcaat aaggacttca ggttgattgt
      601 tttgaagttc tctttctttc atattgttca caaaatcaat gtttttactt cttgcggttt
      661 catttgtgct tgcaattgcc tttgaaatag tgctttgttg atagtcttct cttgtattcc
      721 atttcttttg gtggtagtca tcttttctct gaaaataagg actttgttca aaatataatt
      781 tcataagtgt ttcattttta tttgtccaaa atgcaatttt gttcatcaat gcttggtcgg
      841 tttctgattc attccctcca cttcctgaag gtgtttcatt ccatagttga atcaatttgt
      901 catctttttc aagtcctatc ttgaagaatt gttcatctga tttcattcct tcatcataag
      961 tgattgaagt ttgatttgta ggttggaaag tgttttcagg tcttttcata tatgtttcaa
     1021 tgatttcttg tagttcttct cttgatattt ctcttatttc atcaattttc aagtatttat
     1081 tccctgtcaa agtcaagtat ctgtt
//
